Tusp cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tusp
Entrez Gene ID	43317
Full Name	CG5586 gene product from transcript CG5586-RE
Synonyms	CG5586,DMG2-MB6.chr3R.24.043,Dmel\CG5586,TUSP
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 98A10-98A12

mRNA and Protein(s)

mRNA	Protein	Name
NM_001276086.2	NP_001263015.1	tusp, isoform G
NM_143316.4	NP_651573.4	tusp, isoform D
NM_001202374.2	NP_001189303.1	tusp, isoform E
NM_001276085.2	NP_001263014.1	tusp, isoform F

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001716	XP_321370.3
Drosophila melanogaster (fruit fly)	Tusp	NP_001263014.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data suggest TUSP is a novel regulator of SNARE assembly and, therefore, of neurotransmitter release.
Title: Tubby domain superfamily protein is required for the formation of the 7S SNARE complex in Drosophila.

The following Tusp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tusp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06341	NM_001202374.2 Latest version!	Drosophila melanogaster tusp, transcript variant E (Tusp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa06341	NM_143316.4 Latest version!	Drosophila melanogaster tusp, transcript variant D (Tusp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa08916	NM_001276085.2 Latest version!	Drosophila melanogaster tusp, transcript variant F (Tusp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa08921	NM_001276086.2 Latest version!	Drosophila melanogaster tusp, transcript variant G (Tusp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tusp ( NM_143316.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651573.4 Drosophila melanogaster tusp, transcript variant D (Tusp), mRNA.

Price & Availability↑

CloneID	OFa06341
Clone ID Related Accession (Same CDS sequence)	NM_143316.4 , NM_001202374.2
Accession Version	NM_143316.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tusp, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 31, 2011 this sequence version replaced NM_143316.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATTTAC ACTTTGAGCG AAACATCAAC ACAAAATGCG ACTGCACGAT ACTGTCATTA 
TCATGGATGG GAAAGGTGCC AGATGATATA CCCGAGGACG AGGGATGGAA GTTGAACCGC 
ACCAACTACT ACCAGGAGGG ATGGCTGGCC ACGGGCAATG TGCGCGGCAT TGTGGGCGTA 
ACCTTTACCA CCTCACATTG CCGCAAGAAC ATGGACTATC CGTTGAGGAC CAACTACAAC 
CTGCGCGGGC ATAGATCGGA TGTTATCCTG GTCAAGTGGA ATGAGCCGTA TCAAAAGCTA 
GCCTCCTGCG ATAGTTCGGG CATCATCTTT GTGTGGATCA AGTACGAGGG TCGCTGGTCC 
ATCGAGCTGA TCAACGATCG GAACACACCG GTGACCCACT TCTCCTGGTC ACACGATGGC 
CGCATGGCGC TGATCTGCTA CCAGGATGGC TTCGTTCTGG TAGGATCCGT GGCTGGGCAG 
CGATATTGGT CATCCATGCT CAATCTAGAG TCCACCATTA CCTGCGGCAT TTGGACGCCC 
GACGACCAGC AGGTGTACTT CGGCACAACC CAGGGTCAGG TTATTGTGAT GGATGTTCAC 
GGAGCGATGG TGTCGCAGGT TCAGCTGTCC AACGATGTGC CCATCACCTC GATGGCCTGG 
TCCTGCGAGA AGTTCAAGAT GGAGGAGGGC GAGGAGGCGG AGCCCGGTGT AACCAATGCG 
GCCAAGCGTT CCTTTGTCCT GGCAGTAAGC TTCCAGAATG GATATATCTA TTTGCTAAAG 
TCGTTTGACG ACGTCTCACC CGCACATATC AACACATGCC TTAATGGCGC CCTTGGCATG 
GTCATGGAGT GGAGCAACTC TCGCGAGCTG CTCGCCGTCG CGGGAACCCT GCGAACGGGA 
GTCGACGGAA CGAAGACAGA AGACATGGGC ACGCCCAGTT GCTACACGAA TCTGGTCAAG 
TTCTACACGG AAACGGGCAC CTGTCTGTAT CAGGCTCACA TTCCATGCAG CTCCGCCACC 
GTTTCGGCCA TCACCTGGGG CCACAACGAC AAGCGACTGT TCATCGCCAC GGGGACACAG 
GTTCACATCG CCTGGGTCTC CAGACGGGTG GCCTCCCTGC AGCTGCTGTG CCGCCTCGAG 
ATCCAGGCGA GCGTCGGATC CGAGTCGCTG CTGCCCCTGC TGCCGTTGCC TTCTAGGATT 
AAGTCTCTCA TTGGCAATCT GTTTGCTCAA ACGATAAGAT GCTGTGTACC TGACCTCAAG 
TCGCTGCGGG ATTTTGTTTC GCGACCACCG CTCTGCTCCA CTCGGCTGCA CTGCACCATG 
ATCCGGCACG ACGACGACTC CAATCTGAGC TCCGGCACAT GCTATACGCT GTATCTGGAG 
TATCTGGGCG GCTTGGTGCC GCTGCTCAAG GGCAAGCGCA CCTCCAAGAT TCGTCCTGAG 
TTTGTAATCT TCGATCCCCA AGTTAATGAT TCCCCCTTGT ACTTTCAATA CTCCGCCGAG 
GCCAAGAGCT CCTCGGGCTC CAGCCAGTCC ACCACCACGG GGAACAGTGG GCGCACCGAC 
TCATCAGACA GTGACTTCGA GGAGAGATCA CGGTTTGGCT CCCCACGCAC TCCTCGCAAG 
AAGCGAGTGC GTCCAAAGAG ACGCCATCAG GCGGGCGATC GGCTGAGCGC TTCTGGTGGC 
GGGGTAAGCA ACGATCCCGA TAGCCTGGAT GAACTGGCCT ATGTGGACAC ACTGCCGGAG 
GAAGTCAAGC TGGTCGAGGT GACCTCCAAC ATTTGGGGAA CCAAGTTCAA GATCCATGGA 
CTTGCCAAAA CCGTCCCAGC CAATTTAGGC CAAGTAACTT ATAAGACGTC CCTACTGCAT 
TTGCAGCCGC GTCAAATGAC GCTGGTCATC ACCGAGCTGC GCGACGATTT TCCACCTGGT 
CCCGATCCCA GCTTTAATCC GAACATATTC TCTGAGGACG AGGATGAGCA TCACCAGCAC 
CAGGGAATAC ATCACGATGC AGTGCCGCAG GTTAATGTGA TCACCGCTCA GGATGGGGCT 
TCCTTAAAGC CGCCTATAAT ACCGCAACGT CGCCTCACCG ATGGAGCATC TGCACCATTG 
ATAGCGCCCA TGTCACCACG TCCAAATCGA ATCCTGGCGC GACACAAGAA CTCATTGACT 
GTGAACGGAG AACGAGGATC TGGCTCGTCG GCGGGCTTGA GTCCCCTAGC GCGAGCGGAG 
AGCTACGATG ATGATTCATC CAATGAATCC CAGGAGGCAG GAGCTGCAGG CAGCCAGTCA 
ACCACTGTGC TACTGCACCA GGCGCCCTCG AATGGCTCGG GTCCTGGACC CAGCTGCAGT 
AAGAGCATAA CGCGTCCCAA AACTATCAGC AGCTTCAAAA ACAGCTACAG CCGCTCCAGC 
TCCAATTCCA GCTGCCAGTC GCGTCACGCC ATCTCGCCAC TGTACTGCGA TGGAGCAGTG 
CCCACGCTGC AGAGTCCCAA GAATGCAGTG GCTCCTTCGG ACATCATCTT CGAGCGTCCA 
GCAGTGCCCG CCGCTGGCCA GACCACCCTG ATGTCCTACT CGAGCAATGC GGACTATGCC 
AATAATGTGG TTCAGGTGAA GAATGCTCTG ATGTCGGAGC CCGTGCGCTC GGCCAACAGC 
CATGTGAATC CAGTGCCGCT GAACCTCAAT CTAAATCTGG AGCGAATGGA TGCACGGGTT 
AAGTGCACGG CACCAACCAC ATCTTCGACG GCCAAGCGAC GGGAAATGTT GTATATCGAT 
GAGGAGACGC AGTCGCCAAC GCCTACGCCA AGTAGCAGCA GCATGAAACG AACGCCAACG 
GTGGTTTCAA TAGCTCCTGC TTTGCCCGAT TCCATCACAC GCAGCTGCAG CGTCGGCTAT 
CTGGATTCGG TTGCCATCAC GCCATCCGAC GAAGCGCTGT CCGCTCTGCG TAAGGATGCG 
CCAAACAAGC GGCTGATATT GGTGGACAAG AAGAGGAATC GCCGGAAAAG GCAACAGCAG 
GAGGATGCCC GGCGCCACAA GCTGCAGCAA ACTGGGAAGT CCAAGAGCCT AGACTCCTGC 
GACCTGTTGT CCCTACAGAC TAAGCTGTCC AGCAAGGAGC ACGAGCAGGT GGTGCGCAAG 
CTGCAGGAGA TATCCGATAG CAGCGCCTGC AGCAGTGCGG CCAACACATT GTGCTTCAAG 
TGCCGCAACA ACATGAGCCC CAGCAGTGCC TGCAAACGGT GTCAGCCCTC GGCGAGCTCC 
GTCCTGGATG AGATTACAGC AGTCGTGCCC GCGGTGGAGC CGGCCAAGGA GTCTCCTGCG 
GTGCAGGCCA AGCCAGCGCC CAAGAAACGC TTCGATGTTA TCACTAGCTT CACAGACTCG 
CCACTTTTTA CGAGGAAACA TCGATTTGGA ATCGGCCGCA GTAAGGAAAC GGCTGGCACT 
GAAAACTCCA CTCCACTGCT GGGCAGAAAG CAGGACAATG GCTTCAGCTT TGTGAAGCAG 
CTGTCCGAGG TGCGTTGGAG GCGCAAGGAA CAGCCAGCAC AGGCGCAAGT TAATGGTTCT 
AGTAATGCCA GTACTTTAGA GCGTCAGCCA CAGCCGGAGA TCACAGGTGC CGCTTGTGGC 
ACAGTGGAAG CTACACCAGT GGAGGCGAAG GCTTCGGTTT CTCTGCATAC TCAGGCTCTC 
ACCACTTTAG AGAACATAAT AAGCCGCCTT CGAGATCTAG ACGAGGGTCG TTTGACGCCT 
CCTTCAACCC CACAACGACT GCCTCGAAGT TCTCCTGCAT CTCCGGCAGC CAGCAAAAAG 
AACAAAAGGC AGCAGAGCAA CTCTCCCATT CGCCACATAT TGAATTCACC GCTTTTGAAT 
CGGCGTCAGC GCAAAAAACA GAGCATTATC GAGAGCTCCG ATGATGAGGG TAACCAGACC 
AATGGATCCG GTGAGGAGAG CAACAATGCG GGCAATGGCA AGCAGTATCG CGACCTGGAG 
ACCTTCCAAA AAGCTCAACT GCGTCAGAAG CTAAAGCGCG GCAAGATTGA ACCCAATGGC 
AGTGCTAGTT GTGCCAATCC GGCGCCAGTG CGTCGTGAAT TTGTGATGCA CAACAAGGCG 
CCCATGTGGA ATGAGATGAG TCAGGTGTAC CAGCTGGACT TTGGCGGACG CGTTACCCAG 
GAGTCAGCCA AGAACTTCCA AATCGAGTTT CGTGGCAAGC AGGTCATGCA ATTCGGTCGC 
ATTGATGGAA ATGCCTATAC CCTGGATTTC CAGTATCCTT TCTCCGCCCT CCAGGCCTTC 
GCCGTGGCTC TGGCCAATGT GACACAGCGA CTTAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651573.4
CDS	194..4492
Translation	MHLHFERNINTKCDCTILSLSWMGKVPDDIPEDEGWKLNRTNYYQEGWLATGNVRGIVGVTFTTSHCRKNMDYPLRTNYNLRGHRSDVILVKWNEPYQKLASCDSSGIIFVWIKYEGRWSIELINDRNTPVTHFSWSHDGRMALICYQDGFVLVGSVAGQRYWSSMLNLESTITCGIWTPDDQQVYFGTTQGQVIVMDVHGAMVSQVQLSNDVPITSMAWSCEKFKMEEGEEAEPGVTNAAKRSFVLAVSFQNGYIYLLKSFDDVSPAHINTCLNGALGMVMEWSNSRELLAVAGTLRTGVDGTKTEDMGTPSCYTNLVKFYTETGTCLYQAHIPCSSATVSAITWGHNDKRLFIATGTQVHIAWVSRRVASLQLLCRLEIQASVGSESLLPLLPLPSRIKSLIGNLFAQTIRCCVPDLKSLRDFVSRPPLCSTRLHCTMIRHDDDSNLSSGTCYTLYLEYLGGLVPLLKGKRTSKIRPEFVIFDPQVNDSPLYFQYSAEAKSSSGSSQSTTTGNSGRTDSSDSDFEERSRFGSPRTPRKKRVRPKRRHQAGDRLSASGGGVSNDPDSLDELAYVDTLPEEVKLVEVTSNIWGTKFKIHGLAKTVPANLGQVTYKTSLLHLQPRQMTLVITELRDDFPPGPDPSFNPNIFSEDEDEHHQHQGIHHDAVPQVNVITAQDGASLKPPIIPQRRLTDGASAPLIAPMSPRPNRILARHKNSLTVNGERGSGSSAGLSPLARAESYDDDSSNESQEAGAAGSQSTTVLLHQAPSNGSGPGPSCSKSITRPKTISSFKNSYSRSSSNSSCQSRHAISPLYCDGAVPTLQSPKNAVAPSDIIFERPAVPAAGQTTLMSYSSNADYANNVVQVKNALMSEPVRSANSHVNPVPLNLNLNLERMDARVKCTAPTTSSTAKRREMLYIDEETQSPTPTPSSSSMKRTPTVVSIAPALPDSITRSCSVGYLDSVAITPSDEALSALRKDAPNKRLILVDKKRNRRKRQQQEDARRHKLQQTGKSKSLDSCDLLSLQTKLSSKEHEQVVRKLQEISDSSACSSAANTLCFKCRNNMSPSSACKRCQPSASSVLDEITAVVPAVEPAKESPAVQAKPAPKKRFDVITSFTDSPLFTRKHRFGIGRSKETAGTENSTPLLGRKQDNGFSFVKQLSEVRWRRKEQPAQAQVNGSSNASTLERQPQPEITGAACGTVEATPVEAKASVSLHTQALTTLENIISRLRDLDEGRLTPPSTPQRLPRSSPASPAASKKNKRQQSNSPIRHILNSPLLNRRQRKKQSIIESSDDEGNQTNGSGEESNNAGNGKQYRDLETFQKAQLRQKLKRGKIEPNGSASCANPAPVRREFVMHNKAPMWNEMSQVYQLDFGGRVTQESAKNFQIEFRGKQVMQFGRIDGNAYTLDFQYPFSALQAFAVALANVTQRLK

Target ORF information:

RefSeq Version	NM_143316.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tusp, transcript variant D (Tusp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143316.4

ORF Insert Sequence:

ATGCATTTAC ACTTTGAGCG AAACATCAAC ACAAAATGCG ACTGCACGAT ACTGTCATTA 
TCATGGATGG GAAAGGTGCC AGATGATATA CCCGAGGACG AGGGATGGAA GTTGAACCGC 
ACCAACTACT ACCAGGAGGG ATGGCTGGCC ACGGGCAATG TGCGCGGCAT TGTGGGCGTA 
ACCTTTACCA CCTCACATTG CCGCAAGAAC ATGGACTATC CGTTGAGGAC CAACTACAAC 
CTGCGCGGGC ATAGATCGGA TGTTATCCTG GTCAAGTGGA ATGAGCCGTA TCAAAAGCTA 
GCCTCCTGCG ATAGTTCGGG CATCATCTTT GTGTGGATCA AGTACGAGGG TCGCTGGTCC 
ATCGAGCTGA TCAACGATCG GAACACACCG GTGACCCACT TCTCCTGGTC ACACGATGGC 
CGCATGGCGC TGATCTGCTA CCAGGATGGC TTCGTTCTGG TAGGATCCGT GGCTGGGCAG 
CGATATTGGT CATCCATGCT CAATCTAGAG TCCACCATTA CCTGCGGCAT TTGGACGCCC 
GACGACCAGC AGGTGTACTT CGGCACAACC CAGGGTCAGG TTATTGTGAT GGATGTTCAC 
GGAGCGATGG TGTCGCAGGT TCAGCTGTCC AACGATGTGC CCATCACCTC GATGGCCTGG 
TCCTGCGAGA AGTTCAAGAT GGAGGAGGGC GAGGAGGCGG AGCCCGGTGT AACCAATGCG 
GCCAAGCGTT CCTTTGTCCT GGCAGTAAGC TTCCAGAATG GATATATCTA TTTGCTAAAG 
TCGTTTGACG ACGTCTCACC CGCACATATC AACACATGCC TTAATGGCGC CCTTGGCATG 
GTCATGGAGT GGAGCAACTC TCGCGAGCTG CTCGCCGTCG CGGGAACCCT GCGAACGGGA 
GTCGACGGAA CGAAGACAGA AGACATGGGC ACGCCCAGTT GCTACACGAA TCTGGTCAAG 
TTCTACACGG AAACGGGCAC CTGTCTGTAT CAGGCTCACA TTCCATGCAG CTCCGCCACC 
GTTTCGGCCA TCACCTGGGG CCACAACGAC AAGCGACTGT TCATCGCCAC GGGGACACAG 
GTTCACATCG CCTGGGTCTC CAGACGGGTG GCCTCCCTGC AGCTGCTGTG CCGCCTCGAG 
ATCCAGGCGA GCGTCGGATC CGAGTCGCTG CTGCCCCTGC TGCCGTTGCC TTCTAGGATT 
AAGTCTCTCA TTGGCAATCT GTTTGCTCAA ACGATAAGAT GCTGTGTACC TGACCTCAAG 
TCGCTGCGGG ATTTTGTTTC GCGACCACCG CTCTGCTCCA CTCGGCTGCA CTGCACCATG 
ATCCGGCACG ACGACGACTC CAATCTGAGC TCCGGCACAT GCTATACGCT GTATCTGGAG 
TATCTGGGCG GCTTGGTGCC GCTGCTCAAG GGCAAGCGCA CCTCCAAGAT TCGTCCTGAG 
TTTGTAATCT TCGATCCCCA AGTTAATGAT TCCCCCTTGT ACTTTCAATA CTCCGCCGAG 
GCCAAGAGCT CCTCGGGCTC CAGCCAGTCC ACCACCACGG GGAACAGTGG GCGCACCGAC 
TCATCAGACA GTGACTTCGA GGAGAGATCA CGGTTTGGCT CCCCACGCAC TCCTCGCAAG 
AAGCGAGTGC GTCCAAAGAG ACGCCATCAG GCGGGCGATC GGCTGAGCGC TTCTGGTGGC 
GGGGTAAGCA ACGATCCCGA TAGCCTGGAT GAACTGGCCT ATGTGGACAC ACTGCCGGAG 
GAAGTCAAGC TGGTCGAGGT GACCTCCAAC ATTTGGGGAA CCAAGTTCAA GATCCATGGA 
CTTGCCAAAA CCGTCCCAGC CAATTTAGGC CAAGTAACTT ATAAGACGTC CCTACTGCAT 
TTGCAGCCGC GTCAAATGAC GCTGGTCATC ACCGAGCTGC GCGACGATTT TCCACCTGGT 
CCCGATCCCA GCTTTAATCC GAACATATTC TCTGAGGACG AGGATGAGCA TCACCAGCAC 
CAGGGAATAC ATCACGATGC AGTGCCGCAG GTTAATGTGA TCACCGCTCA GGATGGGGCT 
TCCTTAAAGC CGCCTATAAT ACCGCAACGT CGCCTCACCG ATGGAGCATC TGCACCATTG 
ATAGCGCCCA TGTCACCACG TCCAAATCGA ATCCTGGCGC GACACAAGAA CTCATTGACT 
GTGAACGGAG AACGAGGATC TGGCTCGTCG GCGGGCTTGA GTCCCCTAGC GCGAGCGGAG 
AGCTACGATG ATGATTCATC CAATGAATCC CAGGAGGCAG GAGCTGCAGG CAGCCAGTCA 
ACCACTGTGC TACTGCACCA GGCGCCCTCG AATGGCTCGG GTCCTGGACC CAGCTGCAGT 
AAGAGCATAA CGCGTCCCAA AACTATCAGC AGCTTCAAAA ACAGCTACAG CCGCTCCAGC 
TCCAATTCCA GCTGCCAGTC GCGTCACGCC ATCTCGCCAC TGTACTGCGA TGGAGCAGTG 
CCCACGCTGC AGAGTCCCAA GAATGCAGTG GCTCCTTCGG ACATCATCTT CGAGCGTCCA 
GCAGTGCCCG CCGCTGGCCA GACCACCCTG ATGTCCTACT CGAGCAATGC GGACTATGCC 
AATAATGTGG TTCAGGTGAA GAATGCTCTG ATGTCGGAGC CCGTGCGCTC GGCCAACAGC 
CATGTGAATC CAGTGCCGCT GAACCTCAAT CTAAATCTGG AGCGAATGGA TGCACGGGTT 
AAGTGCACGG CACCAACCAC ATCTTCGACG GCCAAGCGAC GGGAAATGTT GTATATCGAT 
GAGGAGACGC AGTCGCCAAC GCCTACGCCA AGTAGCAGCA GCATGAAACG AACGCCAACG 
GTGGTTTCAA TAGCTCCTGC TTTGCCCGAT TCCATCACAC GCAGCTGCAG CGTCGGCTAT 
CTGGATTCGG TTGCCATCAC GCCATCCGAC GAAGCGCTGT CCGCTCTGCG TAAGGATGCG 
CCAAACAAGC GGCTGATATT GGTGGACAAG AAGAGGAATC GCCGGAAAAG GCAACAGCAG 
GAGGATGCCC GGCGCCACAA GCTGCAGCAA ACTGGGAAGT CCAAGAGCCT AGACTCCTGC 
GACCTGTTGT CCCTACAGAC TAAGCTGTCC AGCAAGGAGC ACGAGCAGGT GGTGCGCAAG 
CTGCAGGAGA TATCCGATAG CAGCGCCTGC AGCAGTGCGG CCAACACATT GTGCTTCAAG 
TGCCGCAACA ACATGAGCCC CAGCAGTGCC TGCAAACGGT GTCAGCCCTC GGCGAGCTCC 
GTCCTGGATG AGATTACAGC AGTCGTGCCC GCGGTGGAGC CGGCCAAGGA GTCTCCTGCG 
GTGCAGGCCA AGCCAGCGCC CAAGAAACGC TTCGATGTTA TCACTAGCTT CACAGACTCG 
CCACTTTTTA CGAGGAAACA TCGATTTGGA ATCGGCCGCA GTAAGGAAAC GGCTGGCACT 
GAAAACTCCA CTCCACTGCT GGGCAGAAAG CAGGACAATG GCTTCAGCTT TGTGAAGCAG 
CTGTCCGAGG TGCGTTGGAG GCGCAAGGAA CAGCCAGCAC AGGCGCAAGT TAATGGTTCT 
AGTAATGCCA GTACTTTAGA GCGTCAGCCA CAGCCGGAGA TCACAGGTGC CGCTTGTGGC 
ACAGTGGAAG CTACACCAGT GGAGGCGAAG GCTTCGGTTT CTCTGCATAC TCAGGCTCTC 
ACCACTTTAG AGAACATAAT AAGCCGCCTT CGAGATCTAG ACGAGGGTCG TTTGACGCCT 
CCTTCAACCC CACAACGACT GCCTCGAAGT TCTCCTGCAT CTCCGGCAGC CAGCAAAAAG 
AACAAAAGGC AGCAGAGCAA CTCTCCCATT CGCCACATAT TGAATTCACC GCTTTTGAAT 
CGGCGTCAGC GCAAAAAACA GAGCATTATC GAGAGCTCCG ATGATGAGGG TAACCAGACC 
AATGGATCCG GTGAGGAGAG CAACAATGCG GGCAATGGCA AGCAGTATCG CGACCTGGAG 
ACCTTCCAAA AAGCTCAACT GCGTCAGAAG CTAAAGCGCG GCAAGATTGA ACCCAATGGC 
AGTGCTAGTT GTGCCAATCC GGCGCCAGTG CGTCGTGAAT TTGTGATGCA CAACAAGGCG 
CCCATGTGGA ATGAGATGAG TCAGGTGTAC CAGCTGGACT TTGGCGGACG CGTTACCCAG 
GAGTCAGCCA AGAACTTCCA AATCGAGTTT CGTGGCAAGC AGGTCATGCA ATTCGGTCGC 
ATTGATGGAA ATGCCTATAC CCTGGATTTC CAGTATCCTT TCTCCGCCCT CCAGGCCTTC 
GCCGTGGCTC TGGCCAATGT GACACAGCGA CTTAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tusp ( NM_001202374.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189303.1 Drosophila melanogaster tusp, transcript variant E (Tusp), mRNA.

Price & Availability↑

CloneID	OFa06341
Clone ID Related Accession (Same CDS sequence)	NM_143316.4 , NM_001202374.2
Accession Version	NM_001202374.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tusp, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001202374.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATTTAC ACTTTGAGCG AAACATCAAC ACAAAATGCG ACTGCACGAT ACTGTCATTA 
TCATGGATGG GAAAGGTGCC AGATGATATA CCCGAGGACG AGGGATGGAA GTTGAACCGC 
ACCAACTACT ACCAGGAGGG ATGGCTGGCC ACGGGCAATG TGCGCGGCAT TGTGGGCGTA 
ACCTTTACCA CCTCACATTG CCGCAAGAAC ATGGACTATC CGTTGAGGAC CAACTACAAC 
CTGCGCGGGC ATAGATCGGA TGTTATCCTG GTCAAGTGGA ATGAGCCGTA TCAAAAGCTA 
GCCTCCTGCG ATAGTTCGGG CATCATCTTT GTGTGGATCA AGTACGAGGG TCGCTGGTCC 
ATCGAGCTGA TCAACGATCG GAACACACCG GTGACCCACT TCTCCTGGTC ACACGATGGC 
CGCATGGCGC TGATCTGCTA CCAGGATGGC TTCGTTCTGG TAGGATCCGT GGCTGGGCAG 
CGATATTGGT CATCCATGCT CAATCTAGAG TCCACCATTA CCTGCGGCAT TTGGACGCCC 
GACGACCAGC AGGTGTACTT CGGCACAACC CAGGGTCAGG TTATTGTGAT GGATGTTCAC 
GGAGCGATGG TGTCGCAGGT TCAGCTGTCC AACGATGTGC CCATCACCTC GATGGCCTGG 
TCCTGCGAGA AGTTCAAGAT GGAGGAGGGC GAGGAGGCGG AGCCCGGTGT AACCAATGCG 
GCCAAGCGTT CCTTTGTCCT GGCAGTAAGC TTCCAGAATG GATATATCTA TTTGCTAAAG 
TCGTTTGACG ACGTCTCACC CGCACATATC AACACATGCC TTAATGGCGC CCTTGGCATG 
GTCATGGAGT GGAGCAACTC TCGCGAGCTG CTCGCCGTCG CGGGAACCCT GCGAACGGGA 
GTCGACGGAA CGAAGACAGA AGACATGGGC ACGCCCAGTT GCTACACGAA TCTGGTCAAG 
TTCTACACGG AAACGGGCAC CTGTCTGTAT CAGGCTCACA TTCCATGCAG CTCCGCCACC 
GTTTCGGCCA TCACCTGGGG CCACAACGAC AAGCGACTGT TCATCGCCAC GGGGACACAG 
GTTCACATCG CCTGGGTCTC CAGACGGGTG GCCTCCCTGC AGCTGCTGTG CCGCCTCGAG 
ATCCAGGCGA GCGTCGGATC CGAGTCGCTG CTGCCCCTGC TGCCGTTGCC TTCTAGGATT 
AAGTCTCTCA TTGGCAATCT GTTTGCTCAA ACGATAAGAT GCTGTGTACC TGACCTCAAG 
TCGCTGCGGG ATTTTGTTTC GCGACCACCG CTCTGCTCCA CTCGGCTGCA CTGCACCATG 
ATCCGGCACG ACGACGACTC CAATCTGAGC TCCGGCACAT GCTATACGCT GTATCTGGAG 
TATCTGGGCG GCTTGGTGCC GCTGCTCAAG GGCAAGCGCA CCTCCAAGAT TCGTCCTGAG 
TTTGTAATCT TCGATCCCCA AGTTAATGAT TCCCCCTTGT ACTTTCAATA CTCCGCCGAG 
GCCAAGAGCT CCTCGGGCTC CAGCCAGTCC ACCACCACGG GGAACAGTGG GCGCACCGAC 
TCATCAGACA GTGACTTCGA GGAGAGATCA CGGTTTGGCT CCCCACGCAC TCCTCGCAAG 
AAGCGAGTGC GTCCAAAGAG ACGCCATCAG GCGGGCGATC GGCTGAGCGC TTCTGGTGGC 
GGGGTAAGCA ACGATCCCGA TAGCCTGGAT GAACTGGCCT ATGTGGACAC ACTGCCGGAG 
GAAGTCAAGC TGGTCGAGGT GACCTCCAAC ATTTGGGGAA CCAAGTTCAA GATCCATGGA 
CTTGCCAAAA CCGTCCCAGC CAATTTAGGC CAAGTAACTT ATAAGACGTC CCTACTGCAT 
TTGCAGCCGC GTCAAATGAC GCTGGTCATC ACCGAGCTGC GCGACGATTT TCCACCTGGT 
CCCGATCCCA GCTTTAATCC GAACATATTC TCTGAGGACG AGGATGAGCA TCACCAGCAC 
CAGGGAATAC ATCACGATGC AGTGCCGCAG GTTAATGTGA TCACCGCTCA GGATGGGGCT 
TCCTTAAAGC CGCCTATAAT ACCGCAACGT CGCCTCACCG ATGGAGCATC TGCACCATTG 
ATAGCGCCCA TGTCACCACG TCCAAATCGA ATCCTGGCGC GACACAAGAA CTCATTGACT 
GTGAACGGAG AACGAGGATC TGGCTCGTCG GCGGGCTTGA GTCCCCTAGC GCGAGCGGAG 
AGCTACGATG ATGATTCATC CAATGAATCC CAGGAGGCAG GAGCTGCAGG CAGCCAGTCA 
ACCACTGTGC TACTGCACCA GGCGCCCTCG AATGGCTCGG GTCCTGGACC CAGCTGCAGT 
AAGAGCATAA CGCGTCCCAA AACTATCAGC AGCTTCAAAA ACAGCTACAG CCGCTCCAGC 
TCCAATTCCA GCTGCCAGTC GCGTCACGCC ATCTCGCCAC TGTACTGCGA TGGAGCAGTG 
CCCACGCTGC AGAGTCCCAA GAATGCAGTG GCTCCTTCGG ACATCATCTT CGAGCGTCCA 
GCAGTGCCCG CCGCTGGCCA GACCACCCTG ATGTCCTACT CGAGCAATGC GGACTATGCC 
AATAATGTGG TTCAGGTGAA GAATGCTCTG ATGTCGGAGC CCGTGCGCTC GGCCAACAGC 
CATGTGAATC CAGTGCCGCT GAACCTCAAT CTAAATCTGG AGCGAATGGA TGCACGGGTT 
AAGTGCACGG CACCAACCAC ATCTTCGACG GCCAAGCGAC GGGAAATGTT GTATATCGAT 
GAGGAGACGC AGTCGCCAAC GCCTACGCCA AGTAGCAGCA GCATGAAACG AACGCCAACG 
GTGGTTTCAA TAGCTCCTGC TTTGCCCGAT TCCATCACAC GCAGCTGCAG CGTCGGCTAT 
CTGGATTCGG TTGCCATCAC GCCATCCGAC GAAGCGCTGT CCGCTCTGCG TAAGGATGCG 
CCAAACAAGC GGCTGATATT GGTGGACAAG AAGAGGAATC GCCGGAAAAG GCAACAGCAG 
GAGGATGCCC GGCGCCACAA GCTGCAGCAA ACTGGGAAGT CCAAGAGCCT AGACTCCTGC 
GACCTGTTGT CCCTACAGAC TAAGCTGTCC AGCAAGGAGC ACGAGCAGGT GGTGCGCAAG 
CTGCAGGAGA TATCCGATAG CAGCGCCTGC AGCAGTGCGG CCAACACATT GTGCTTCAAG 
TGCCGCAACA ACATGAGCCC CAGCAGTGCC TGCAAACGGT GTCAGCCCTC GGCGAGCTCC 
GTCCTGGATG AGATTACAGC AGTCGTGCCC GCGGTGGAGC CGGCCAAGGA GTCTCCTGCG 
GTGCAGGCCA AGCCAGCGCC CAAGAAACGC TTCGATGTTA TCACTAGCTT CACAGACTCG 
CCACTTTTTA CGAGGAAACA TCGATTTGGA ATCGGCCGCA GTAAGGAAAC GGCTGGCACT 
GAAAACTCCA CTCCACTGCT GGGCAGAAAG CAGGACAATG GCTTCAGCTT TGTGAAGCAG 
CTGTCCGAGG TGCGTTGGAG GCGCAAGGAA CAGCCAGCAC AGGCGCAAGT TAATGGTTCT 
AGTAATGCCA GTACTTTAGA GCGTCAGCCA CAGCCGGAGA TCACAGGTGC CGCTTGTGGC 
ACAGTGGAAG CTACACCAGT GGAGGCGAAG GCTTCGGTTT CTCTGCATAC TCAGGCTCTC 
ACCACTTTAG AGAACATAAT AAGCCGCCTT CGAGATCTAG ACGAGGGTCG TTTGACGCCT 
CCTTCAACCC CACAACGACT GCCTCGAAGT TCTCCTGCAT CTCCGGCAGC CAGCAAAAAG 
AACAAAAGGC AGCAGAGCAA CTCTCCCATT CGCCACATAT TGAATTCACC GCTTTTGAAT 
CGGCGTCAGC GCAAAAAACA GAGCATTATC GAGAGCTCCG ATGATGAGGG TAACCAGACC 
AATGGATCCG GTGAGGAGAG CAACAATGCG GGCAATGGCA AGCAGTATCG CGACCTGGAG 
ACCTTCCAAA AAGCTCAACT GCGTCAGAAG CTAAAGCGCG GCAAGATTGA ACCCAATGGC 
AGTGCTAGTT GTGCCAATCC GGCGCCAGTG CGTCGTGAAT TTGTGATGCA CAACAAGGCG 
CCCATGTGGA ATGAGATGAG TCAGGTGTAC CAGCTGGACT TTGGCGGACG CGTTACCCAG 
GAGTCAGCCA AGAACTTCCA AATCGAGTTT CGTGGCAAGC AGGTCATGCA ATTCGGTCGC 
ATTGATGGAA ATGCCTATAC CCTGGATTTC CAGTATCCTT TCTCCGCCCT CCAGGCCTTC 
GCCGTGGCTC TGGCCAATGT GACACAGCGA CTTAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189303.1
CDS	794..5092
Translation	MHLHFERNINTKCDCTILSLSWMGKVPDDIPEDEGWKLNRTNYYQEGWLATGNVRGIVGVTFTTSHCRKNMDYPLRTNYNLRGHRSDVILVKWNEPYQKLASCDSSGIIFVWIKYEGRWSIELINDRNTPVTHFSWSHDGRMALICYQDGFVLVGSVAGQRYWSSMLNLESTITCGIWTPDDQQVYFGTTQGQVIVMDVHGAMVSQVQLSNDVPITSMAWSCEKFKMEEGEEAEPGVTNAAKRSFVLAVSFQNGYIYLLKSFDDVSPAHINTCLNGALGMVMEWSNSRELLAVAGTLRTGVDGTKTEDMGTPSCYTNLVKFYTETGTCLYQAHIPCSSATVSAITWGHNDKRLFIATGTQVHIAWVSRRVASLQLLCRLEIQASVGSESLLPLLPLPSRIKSLIGNLFAQTIRCCVPDLKSLRDFVSRPPLCSTRLHCTMIRHDDDSNLSSGTCYTLYLEYLGGLVPLLKGKRTSKIRPEFVIFDPQVNDSPLYFQYSAEAKSSSGSSQSTTTGNSGRTDSSDSDFEERSRFGSPRTPRKKRVRPKRRHQAGDRLSASGGGVSNDPDSLDELAYVDTLPEEVKLVEVTSNIWGTKFKIHGLAKTVPANLGQVTYKTSLLHLQPRQMTLVITELRDDFPPGPDPSFNPNIFSEDEDEHHQHQGIHHDAVPQVNVITAQDGASLKPPIIPQRRLTDGASAPLIAPMSPRPNRILARHKNSLTVNGERGSGSSAGLSPLARAESYDDDSSNESQEAGAAGSQSTTVLLHQAPSNGSGPGPSCSKSITRPKTISSFKNSYSRSSSNSSCQSRHAISPLYCDGAVPTLQSPKNAVAPSDIIFERPAVPAAGQTTLMSYSSNADYANNVVQVKNALMSEPVRSANSHVNPVPLNLNLNLERMDARVKCTAPTTSSTAKRREMLYIDEETQSPTPTPSSSSMKRTPTVVSIAPALPDSITRSCSVGYLDSVAITPSDEALSALRKDAPNKRLILVDKKRNRRKRQQQEDARRHKLQQTGKSKSLDSCDLLSLQTKLSSKEHEQVVRKLQEISDSSACSSAANTLCFKCRNNMSPSSACKRCQPSASSVLDEITAVVPAVEPAKESPAVQAKPAPKKRFDVITSFTDSPLFTRKHRFGIGRSKETAGTENSTPLLGRKQDNGFSFVKQLSEVRWRRKEQPAQAQVNGSSNASTLERQPQPEITGAACGTVEATPVEAKASVSLHTQALTTLENIISRLRDLDEGRLTPPSTPQRLPRSSPASPAASKKNKRQQSNSPIRHILNSPLLNRRQRKKQSIIESSDDEGNQTNGSGEESNNAGNGKQYRDLETFQKAQLRQKLKRGKIEPNGSASCANPAPVRREFVMHNKAPMWNEMSQVYQLDFGGRVTQESAKNFQIEFRGKQVMQFGRIDGNAYTLDFQYPFSALQAFAVALANVTQRLK

Target ORF information:

RefSeq Version	NM_001202374.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tusp, transcript variant E (Tusp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202374.2

ORF Insert Sequence:

ATGCATTTAC ACTTTGAGCG AAACATCAAC ACAAAATGCG ACTGCACGAT ACTGTCATTA 
TCATGGATGG GAAAGGTGCC AGATGATATA CCCGAGGACG AGGGATGGAA GTTGAACCGC 
ACCAACTACT ACCAGGAGGG ATGGCTGGCC ACGGGCAATG TGCGCGGCAT TGTGGGCGTA 
ACCTTTACCA CCTCACATTG CCGCAAGAAC ATGGACTATC CGTTGAGGAC CAACTACAAC 
CTGCGCGGGC ATAGATCGGA TGTTATCCTG GTCAAGTGGA ATGAGCCGTA TCAAAAGCTA 
GCCTCCTGCG ATAGTTCGGG CATCATCTTT GTGTGGATCA AGTACGAGGG TCGCTGGTCC 
ATCGAGCTGA TCAACGATCG GAACACACCG GTGACCCACT TCTCCTGGTC ACACGATGGC 
CGCATGGCGC TGATCTGCTA CCAGGATGGC TTCGTTCTGG TAGGATCCGT GGCTGGGCAG 
CGATATTGGT CATCCATGCT CAATCTAGAG TCCACCATTA CCTGCGGCAT TTGGACGCCC 
GACGACCAGC AGGTGTACTT CGGCACAACC CAGGGTCAGG TTATTGTGAT GGATGTTCAC 
GGAGCGATGG TGTCGCAGGT TCAGCTGTCC AACGATGTGC CCATCACCTC GATGGCCTGG 
TCCTGCGAGA AGTTCAAGAT GGAGGAGGGC GAGGAGGCGG AGCCCGGTGT AACCAATGCG 
GCCAAGCGTT CCTTTGTCCT GGCAGTAAGC TTCCAGAATG GATATATCTA TTTGCTAAAG 
TCGTTTGACG ACGTCTCACC CGCACATATC AACACATGCC TTAATGGCGC CCTTGGCATG 
GTCATGGAGT GGAGCAACTC TCGCGAGCTG CTCGCCGTCG CGGGAACCCT GCGAACGGGA 
GTCGACGGAA CGAAGACAGA AGACATGGGC ACGCCCAGTT GCTACACGAA TCTGGTCAAG 
TTCTACACGG AAACGGGCAC CTGTCTGTAT CAGGCTCACA TTCCATGCAG CTCCGCCACC 
GTTTCGGCCA TCACCTGGGG CCACAACGAC AAGCGACTGT TCATCGCCAC GGGGACACAG 
GTTCACATCG CCTGGGTCTC CAGACGGGTG GCCTCCCTGC AGCTGCTGTG CCGCCTCGAG 
ATCCAGGCGA GCGTCGGATC CGAGTCGCTG CTGCCCCTGC TGCCGTTGCC TTCTAGGATT 
AAGTCTCTCA TTGGCAATCT GTTTGCTCAA ACGATAAGAT GCTGTGTACC TGACCTCAAG 
TCGCTGCGGG ATTTTGTTTC GCGACCACCG CTCTGCTCCA CTCGGCTGCA CTGCACCATG 
ATCCGGCACG ACGACGACTC CAATCTGAGC TCCGGCACAT GCTATACGCT GTATCTGGAG 
TATCTGGGCG GCTTGGTGCC GCTGCTCAAG GGCAAGCGCA CCTCCAAGAT TCGTCCTGAG 
TTTGTAATCT TCGATCCCCA AGTTAATGAT TCCCCCTTGT ACTTTCAATA CTCCGCCGAG 
GCCAAGAGCT CCTCGGGCTC CAGCCAGTCC ACCACCACGG GGAACAGTGG GCGCACCGAC 
TCATCAGACA GTGACTTCGA GGAGAGATCA CGGTTTGGCT CCCCACGCAC TCCTCGCAAG 
AAGCGAGTGC GTCCAAAGAG ACGCCATCAG GCGGGCGATC GGCTGAGCGC TTCTGGTGGC 
GGGGTAAGCA ACGATCCCGA TAGCCTGGAT GAACTGGCCT ATGTGGACAC ACTGCCGGAG 
GAAGTCAAGC TGGTCGAGGT GACCTCCAAC ATTTGGGGAA CCAAGTTCAA GATCCATGGA 
CTTGCCAAAA CCGTCCCAGC CAATTTAGGC CAAGTAACTT ATAAGACGTC CCTACTGCAT 
TTGCAGCCGC GTCAAATGAC GCTGGTCATC ACCGAGCTGC GCGACGATTT TCCACCTGGT 
CCCGATCCCA GCTTTAATCC GAACATATTC TCTGAGGACG AGGATGAGCA TCACCAGCAC 
CAGGGAATAC ATCACGATGC AGTGCCGCAG GTTAATGTGA TCACCGCTCA GGATGGGGCT 
TCCTTAAAGC CGCCTATAAT ACCGCAACGT CGCCTCACCG ATGGAGCATC TGCACCATTG 
ATAGCGCCCA TGTCACCACG TCCAAATCGA ATCCTGGCGC GACACAAGAA CTCATTGACT 
GTGAACGGAG AACGAGGATC TGGCTCGTCG GCGGGCTTGA GTCCCCTAGC GCGAGCGGAG 
AGCTACGATG ATGATTCATC CAATGAATCC CAGGAGGCAG GAGCTGCAGG CAGCCAGTCA 
ACCACTGTGC TACTGCACCA GGCGCCCTCG AATGGCTCGG GTCCTGGACC CAGCTGCAGT 
AAGAGCATAA CGCGTCCCAA AACTATCAGC AGCTTCAAAA ACAGCTACAG CCGCTCCAGC 
TCCAATTCCA GCTGCCAGTC GCGTCACGCC ATCTCGCCAC TGTACTGCGA TGGAGCAGTG 
CCCACGCTGC AGAGTCCCAA GAATGCAGTG GCTCCTTCGG ACATCATCTT CGAGCGTCCA 
GCAGTGCCCG CCGCTGGCCA GACCACCCTG ATGTCCTACT CGAGCAATGC GGACTATGCC 
AATAATGTGG TTCAGGTGAA GAATGCTCTG ATGTCGGAGC CCGTGCGCTC GGCCAACAGC 
CATGTGAATC CAGTGCCGCT GAACCTCAAT CTAAATCTGG AGCGAATGGA TGCACGGGTT 
AAGTGCACGG CACCAACCAC ATCTTCGACG GCCAAGCGAC GGGAAATGTT GTATATCGAT 
GAGGAGACGC AGTCGCCAAC GCCTACGCCA AGTAGCAGCA GCATGAAACG AACGCCAACG 
GTGGTTTCAA TAGCTCCTGC TTTGCCCGAT TCCATCACAC GCAGCTGCAG CGTCGGCTAT 
CTGGATTCGG TTGCCATCAC GCCATCCGAC GAAGCGCTGT CCGCTCTGCG TAAGGATGCG 
CCAAACAAGC GGCTGATATT GGTGGACAAG AAGAGGAATC GCCGGAAAAG GCAACAGCAG 
GAGGATGCCC GGCGCCACAA GCTGCAGCAA ACTGGGAAGT CCAAGAGCCT AGACTCCTGC 
GACCTGTTGT CCCTACAGAC TAAGCTGTCC AGCAAGGAGC ACGAGCAGGT GGTGCGCAAG 
CTGCAGGAGA TATCCGATAG CAGCGCCTGC AGCAGTGCGG CCAACACATT GTGCTTCAAG 
TGCCGCAACA ACATGAGCCC CAGCAGTGCC TGCAAACGGT GTCAGCCCTC GGCGAGCTCC 
GTCCTGGATG AGATTACAGC AGTCGTGCCC GCGGTGGAGC CGGCCAAGGA GTCTCCTGCG 
GTGCAGGCCA AGCCAGCGCC CAAGAAACGC TTCGATGTTA TCACTAGCTT CACAGACTCG 
CCACTTTTTA CGAGGAAACA TCGATTTGGA ATCGGCCGCA GTAAGGAAAC GGCTGGCACT 
GAAAACTCCA CTCCACTGCT GGGCAGAAAG CAGGACAATG GCTTCAGCTT TGTGAAGCAG 
CTGTCCGAGG TGCGTTGGAG GCGCAAGGAA CAGCCAGCAC AGGCGCAAGT TAATGGTTCT 
AGTAATGCCA GTACTTTAGA GCGTCAGCCA CAGCCGGAGA TCACAGGTGC CGCTTGTGGC 
ACAGTGGAAG CTACACCAGT GGAGGCGAAG GCTTCGGTTT CTCTGCATAC TCAGGCTCTC 
ACCACTTTAG AGAACATAAT AAGCCGCCTT CGAGATCTAG ACGAGGGTCG TTTGACGCCT 
CCTTCAACCC CACAACGACT GCCTCGAAGT TCTCCTGCAT CTCCGGCAGC CAGCAAAAAG 
AACAAAAGGC AGCAGAGCAA CTCTCCCATT CGCCACATAT TGAATTCACC GCTTTTGAAT 
CGGCGTCAGC GCAAAAAACA GAGCATTATC GAGAGCTCCG ATGATGAGGG TAACCAGACC 
AATGGATCCG GTGAGGAGAG CAACAATGCG GGCAATGGCA AGCAGTATCG CGACCTGGAG 
ACCTTCCAAA AAGCTCAACT GCGTCAGAAG CTAAAGCGCG GCAAGATTGA ACCCAATGGC 
AGTGCTAGTT GTGCCAATCC GGCGCCAGTG CGTCGTGAAT TTGTGATGCA CAACAAGGCG 
CCCATGTGGA ATGAGATGAG TCAGGTGTAC CAGCTGGACT TTGGCGGACG CGTTACCCAG 
GAGTCAGCCA AGAACTTCCA AATCGAGTTT CGTGGCAAGC AGGTCATGCA ATTCGGTCGC 
ATTGATGGAA ATGCCTATAC CCTGGATTTC CAGTATCCTT TCTCCGCCCT CCAGGCCTTC 
GCCGTGGCTC TGGCCAATGT GACACAGCGA CTTAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tusp ( NM_001276085.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001263014.1 Drosophila melanogaster tusp, transcript variant F (Tusp), mRNA.

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CloneID	OFa08916
Clone ID Related Accession (Same CDS sequence)	NM_001276085.2
Accession Version	NM_001276085.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tusp, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001276085.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATTTAC ACTTTGAGCG AAACATCAAC ACAAAATGCG ACTGCACGAT ACTGTCATTA 
TCATGGATGG GAAAGGTGCC AGATGATATA CCCGAGGACG AGGGATGGAA GTTGAACCGC 
ACCAACTACT ACCAGGAGGG ATGGCTGGCC ACGGGCAATG TGCGCGGCAT TGTGGGCGTA 
ACCTTTACCA CCTCACATTG CCGCAAGAAC ATGGACTATC CGTTGAGGAC CAACTACAAC 
CTGCGCGGGC ATAGATCGGA TGTTATCCTG GTCAAGTGGA ATGAGCCGTA TCAAAAGCTA 
GCCTCCTGCG ATAGTTCGGG CATCATCTTT GTGTGGATCA AGTACGAGGG TCGCTGGTCC 
ATCGAGCTGA TCAACGATCG GAACACACCG GTGACCCACT TCTCCTGGTC ACACGATGGC 
CGCATGGCGC TGATCTGCTA CCAGGATGGC TTCGTTCTGG TAGGATCCGT GGCTGGGCAG 
CGATATTGGT CATCCATGCT CAATCTAGAG TCCACCATTA CCTGCGGCAT TTGGACGCCC 
GACGACCAGC AGGTGTACTT CGGCACAACC CAGGGTCAGG TTATTGTGAT GGATGTTCAC 
GGAGCGATGG TGTCGCAGGT TCAGCTGTCC AACGATGTGC CCATCACCTC GATGGCCTGG 
TCCTGCGAGA AGTTCAAGAT GGAGGAGGGC GAGGAGGCGG AGCCCGGTGT AACCAATGCG 
GCCAAGCGTT CCTTTGTCCT GGCAGTAAGC TTCCAGAATG GATATATCTA TTTGCTAAAG 
TCGTTTGACG ACGTCTCACC CGCACATATC AACACATGCC TTAATGGCGC CCTTGGCATG 
GTCATGGAGT GGAGCAACTC TCGCGAGCTG CTCGCCGTCG CGGGAACCCT GCGAACGGGA 
GTCGACGGAA CGAAGACAGA AGACATGGGC ACGCCCAGTT GCTACACGAA TCTGGTCAAG 
TTCTACACGG AAACGGGCAC CTGTCTGTAT CAGGCTCACA TTCCATGCAG CTCCGCCACC 
GTTTCGGCCA TCACCTGGGG CCACAACGAC AAGCGACTGT TCATCGCCAC GGGGACACAG 
GTTCACATCG CCTGGGTCTC CAGACGGGTG GCCTCCCTGC AGCTGCTGTG CCGCCTCGAG 
ATCCAGGCGA GCGTCGGATC CGAGTCGCTG CTGCCCCTGC TGCCGTTGCC TTCTAGGATT 
AAGTCTCTCA TTGGCAATCT GTTTGCTCAA ACGATAAGAT GCTGTGTACC TGACCTCAAG 
TCGCTGCGGG ATTTTGTTTC GCGACCACCG CTCTGCTCCA CTCGGCTGCA CTGCACCATG 
ATCCGGCACG ACGACGACTC CAATCTGAGC TCCGGCACAT GCTATACGCT GTATCTGGAG 
TATCTGGGCG GCTTGGTGCC GCTGCTCAAG GGCAAGCGCA CCTCCAAGAT TCGTCCTGAG 
TTTGTAATCT TCGATCCCCA AGTTAATGAT TCCCCCTTGT ACTTTCAATA CTCCGCCGAG 
GCCAAGAGCT CCTCGGGCTC CAGCCAGTCC ACCACCACGG GGAACAGTGG GCGCACCGAC 
TCATCAGACA GTGACTTCGA GGAGAGATCA CGGTTTGGCT CCCCACGCAC TCCTCGCAAG 
AAGCGAGTGC GTCCAAAGAG ACGCCATCAG GCGGGCGATC GGCTGAGCGC TTCTGGTGGC 
GGGGTAAGCA ACGATCCCGA TAGCCTGGAT GAACTGGCCT ATGTGGACAC ACTGCCGGAG 
CAGGAAGTCA AGCTGGTCGA GGTGACCTCC AACATTTGGG GAACCAAGTT CAAGATCCAT 
GGACTTGCCA AAACCGTCCC AGCCAATTTA GGCCAAGTAA CTTATAAGAC GTCCCTACTG 
CATTTGCAGC CGCGTCAAAT GACGCTGGTC ATCACCGAGC TGCGCGACGA TTTTCCACCT 
GGTCCCGATC CCAGCTTTAA TCCGAACATA TTCTCTGAGG ACGAGGATGA GCATCACCAG 
CACCAGGGAA TACATCACGA TGCAGTGCCG CAGGTTAATG TGATCACCGC TCAGGATGGG 
GCTTCCTTAA AGCCGCCTAT AATACCGCAA CGTCGCCTCA CCGATGGAGC ATCTGCACCA 
TTGATAGCGC CCATGTCACC ACGTCCAAAT CGAATCCTGG CGCGACACAA GAACTCATTG 
ACTGTGAACG GAGAACGAGG ATCTGGCTCG TCGGCGGGCT TGAGTCCCCT AGCGCGAGCG 
GAGAGCTACG ATGATGATTC ATCCAATGAA TCCCAGGAGG CAGGAGCTGC AGGCAGCCAG 
TCAACCACTG TGCTACTGCA CCAGGCGCCC TCGAATGGCT CGGGTCCTGG ACCCAGCTGC 
AGTAAGAGCA TAACGCGTCC CAAAACTATC AGCAGCTTCA AAAACAGCTA CAGCCGCTCC 
AGCTCCAATT CCAGCTGCCA GTCGCGTCAC GCCATCTCGC CACTGTACTG CGATGGAGCA 
GTGCCCACGC TGCAGAGTCC CAAGAATGCA GTGGCTCCTT CGGACATCAT CTTCGAGCGT 
CCAGCAGTGC CCGCCGCTGG CCAGACCACC CTGATGTCCT ACTCGAGCAA TGCGGACTAT 
GCCAATAATG TGGTTCAGGT GAAGAATGCT CTGATGTCGG AGCCCGTGCG CTCGGCCAAC 
AGCCATGTGA ATCCAGTGCC GCTGAACCTC AATCTAAATC TGGAGCGAAT GGATGCACGG 
GTTAAGTGCA CGGCACCAAC CACATCTTCG ACGGCCAAGC GACGGGAAAT GTTGTATATC 
GATGAGGAGA CGCAGTCGCC AACGCCTACG CCAAGTAGCA GCAGCATGAA ACGAACGCCA 
ACGGTGGTTT CAATAGCTCC TGCTTTGCCC GATTCCATCA CACGCAGCTG CAGCGTCGGC 
TATCTGGATT CGGTTGCCAT CACGCCATCC GACGAAGCGC TGTCCGCTCT GCGTAAGGAT 
GCGCCAAACA AGCGGCTGAT ATTGGTGGAC AAGAAGAGGA ATCGCCGGAA AAGGCAACAG 
CAGGAGGATG CCCGGCGCCA CAAGCTGCAG CAAACTGGGA AGTCCAAGAG CCTAGACTCC 
TGCGACCTGT TGTCCCTACA GACTAAGCTG TCCAGCAAGG AGCACGAGCA GGTGGTGCGC 
AAGCTGCAGG AGATATCCGA TAGCAGCGCC TGCAGCAGTG CGGCCAACAC ATTGTGCTTC 
AAGTGCCGCA ACAACATGAG CCCCAGCAGT GCCTGCAAAC GGTGTCAGCC CTCGGCGAGC 
TCCGTCCTGG ATGAGATTAC AGCAGTCGTG CCCGCGGTGG AGCCGGCCAA GGAGTCTCCT 
GCGGTGCAGG CCAAGCCAGC GCCCAAGAAA CGCTTCGATG TTATCACTAG CTTCACAGAC 
TCGCCACTTT TTACGAGGAA ACATCGATTT GGAATCGGCC GCAGTAAGGA AACGGCTGGC 
ACTGAAAACT CCACTCCACT GCTGGGCAGA AAGCAGGACA ATGGCTTCAG CTTTGTGAAG 
CAGCTGTCCG AGGTGCGTTG GAGGCGCAAG GAACAGCCAG CACAGGCGCA AGTTAATGGT 
TCTAGTAATG CCAGTACTTT AGAGCGTCAG CCACAGCCGG AGATCACAGG TGCCGCTTGT 
GGCACAGTGG AAGCTACACC AGTGGAGGCG AAGGCTTCGG TTTCTCTGCA TACTCAGGCT 
CTCACCACTT TAGAGAACAT AATAAGCCGC CTTCGAGATC TAGACGAGGG TCGTTTGACG 
CCTCCTTCAA CCCCACAACG ACTGCCTCGA AGTTCTCCTG CATCTCCGGC AGCCAGCAAA 
AAGAACAAAA GGCAGCAGAG CAACTCTCCC ATTCGCCACA TATTGAATTC ACCGCTTTTG 
AATCGGCGTC AGCGCAAAAA ACAGAGCATT ATCGAGAGCT CCGATGATGA GGGTAACCAG 
ACCAATGGAT CCGGTGAGGA GAGCAACAAT GCGGGCAATG GCAAGCAGTA TCGCGACCTG 
GAGACCTTCC AAAAAGCTCA ACTGCGTCAG AAGCTAAAGC GCGGCAAGAT TGAACCCAAT 
GGCAGTGCTA GTTGTGCCAA TCCGGCGCCA GTGCGTCGTG AATTTGTGAT GCACAACAAG 
GCGCCCATGT GGAATGAGAT GAGTCAGGTG TACCAGCTGG ACTTTGGCGG ACGCGTTACC 
CAGGAGTCAG CCAAGAACTT CCAAATCGAG TTTCGTGGCA AGCAGGTCAT GCAATTCGGT 
CGCATTGATG GAAATGCCTA TACCCTGGAT TTCCAGTATC CTTTCTCCGC CCTCCAGGCC 
TTCGCCGTGG CTCTGGCCAA TGTGACACAG CGACTTAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263014.1
CDS	428..4729
Translation	MHLHFERNINTKCDCTILSLSWMGKVPDDIPEDEGWKLNRTNYYQEGWLATGNVRGIVGVTFTTSHCRKNMDYPLRTNYNLRGHRSDVILVKWNEPYQKLASCDSSGIIFVWIKYEGRWSIELINDRNTPVTHFSWSHDGRMALICYQDGFVLVGSVAGQRYWSSMLNLESTITCGIWTPDDQQVYFGTTQGQVIVMDVHGAMVSQVQLSNDVPITSMAWSCEKFKMEEGEEAEPGVTNAAKRSFVLAVSFQNGYIYLLKSFDDVSPAHINTCLNGALGMVMEWSNSRELLAVAGTLRTGVDGTKTEDMGTPSCYTNLVKFYTETGTCLYQAHIPCSSATVSAITWGHNDKRLFIATGTQVHIAWVSRRVASLQLLCRLEIQASVGSESLLPLLPLPSRIKSLIGNLFAQTIRCCVPDLKSLRDFVSRPPLCSTRLHCTMIRHDDDSNLSSGTCYTLYLEYLGGLVPLLKGKRTSKIRPEFVIFDPQVNDSPLYFQYSAEAKSSSGSSQSTTTGNSGRTDSSDSDFEERSRFGSPRTPRKKRVRPKRRHQAGDRLSASGGGVSNDPDSLDELAYVDTLPEQEVKLVEVTSNIWGTKFKIHGLAKTVPANLGQVTYKTSLLHLQPRQMTLVITELRDDFPPGPDPSFNPNIFSEDEDEHHQHQGIHHDAVPQVNVITAQDGASLKPPIIPQRRLTDGASAPLIAPMSPRPNRILARHKNSLTVNGERGSGSSAGLSPLARAESYDDDSSNESQEAGAAGSQSTTVLLHQAPSNGSGPGPSCSKSITRPKTISSFKNSYSRSSSNSSCQSRHAISPLYCDGAVPTLQSPKNAVAPSDIIFERPAVPAAGQTTLMSYSSNADYANNVVQVKNALMSEPVRSANSHVNPVPLNLNLNLERMDARVKCTAPTTSSTAKRREMLYIDEETQSPTPTPSSSSMKRTPTVVSIAPALPDSITRSCSVGYLDSVAITPSDEALSALRKDAPNKRLILVDKKRNRRKRQQQEDARRHKLQQTGKSKSLDSCDLLSLQTKLSSKEHEQVVRKLQEISDSSACSSAANTLCFKCRNNMSPSSACKRCQPSASSVLDEITAVVPAVEPAKESPAVQAKPAPKKRFDVITSFTDSPLFTRKHRFGIGRSKETAGTENSTPLLGRKQDNGFSFVKQLSEVRWRRKEQPAQAQVNGSSNASTLERQPQPEITGAACGTVEATPVEAKASVSLHTQALTTLENIISRLRDLDEGRLTPPSTPQRLPRSSPASPAASKKNKRQQSNSPIRHILNSPLLNRRQRKKQSIIESSDDEGNQTNGSGEESNNAGNGKQYRDLETFQKAQLRQKLKRGKIEPNGSASCANPAPVRREFVMHNKAPMWNEMSQVYQLDFGGRVTQESAKNFQIEFRGKQVMQFGRIDGNAYTLDFQYPFSALQAFAVALANVTQRLK

Target ORF information:

RefSeq Version	NM_001276085.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tusp, transcript variant F (Tusp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276085.2

ORF Insert Sequence:

ATGCATTTAC ACTTTGAGCG AAACATCAAC ACAAAATGCG ACTGCACGAT ACTGTCATTA 
TCATGGATGG GAAAGGTGCC AGATGATATA CCCGAGGACG AGGGATGGAA GTTGAACCGC 
ACCAACTACT ACCAGGAGGG ATGGCTGGCC ACGGGCAATG TGCGCGGCAT TGTGGGCGTA 
ACCTTTACCA CCTCACATTG CCGCAAGAAC ATGGACTATC CGTTGAGGAC CAACTACAAC 
CTGCGCGGGC ATAGATCGGA TGTTATCCTG GTCAAGTGGA ATGAGCCGTA TCAAAAGCTA 
GCCTCCTGCG ATAGTTCGGG CATCATCTTT GTGTGGATCA AGTACGAGGG TCGCTGGTCC 
ATCGAGCTGA TCAACGATCG GAACACACCG GTGACCCACT TCTCCTGGTC ACACGATGGC 
CGCATGGCGC TGATCTGCTA CCAGGATGGC TTCGTTCTGG TAGGATCCGT GGCTGGGCAG 
CGATATTGGT CATCCATGCT CAATCTAGAG TCCACCATTA CCTGCGGCAT TTGGACGCCC 
GACGACCAGC AGGTGTACTT CGGCACAACC CAGGGTCAGG TTATTGTGAT GGATGTTCAC 
GGAGCGATGG TGTCGCAGGT TCAGCTGTCC AACGATGTGC CCATCACCTC GATGGCCTGG 
TCCTGCGAGA AGTTCAAGAT GGAGGAGGGC GAGGAGGCGG AGCCCGGTGT AACCAATGCG 
GCCAAGCGTT CCTTTGTCCT GGCAGTAAGC TTCCAGAATG GATATATCTA TTTGCTAAAG 
TCGTTTGACG ACGTCTCACC CGCACATATC AACACATGCC TTAATGGCGC CCTTGGCATG 
GTCATGGAGT GGAGCAACTC TCGCGAGCTG CTCGCCGTCG CGGGAACCCT GCGAACGGGA 
GTCGACGGAA CGAAGACAGA AGACATGGGC ACGCCCAGTT GCTACACGAA TCTGGTCAAG 
TTCTACACGG AAACGGGCAC CTGTCTGTAT CAGGCTCACA TTCCATGCAG CTCCGCCACC 
GTTTCGGCCA TCACCTGGGG CCACAACGAC AAGCGACTGT TCATCGCCAC GGGGACACAG 
GTTCACATCG CCTGGGTCTC CAGACGGGTG GCCTCCCTGC AGCTGCTGTG CCGCCTCGAG 
ATCCAGGCGA GCGTCGGATC CGAGTCGCTG CTGCCCCTGC TGCCGTTGCC TTCTAGGATT 
AAGTCTCTCA TTGGCAATCT GTTTGCTCAA ACGATAAGAT GCTGTGTACC TGACCTCAAG 
TCGCTGCGGG ATTTTGTTTC GCGACCACCG CTCTGCTCCA CTCGGCTGCA CTGCACCATG 
ATCCGGCACG ACGACGACTC CAATCTGAGC TCCGGCACAT GCTATACGCT GTATCTGGAG 
TATCTGGGCG GCTTGGTGCC GCTGCTCAAG GGCAAGCGCA CCTCCAAGAT TCGTCCTGAG 
TTTGTAATCT TCGATCCCCA AGTTAATGAT TCCCCCTTGT ACTTTCAATA CTCCGCCGAG 
GCCAAGAGCT CCTCGGGCTC CAGCCAGTCC ACCACCACGG GGAACAGTGG GCGCACCGAC 
TCATCAGACA GTGACTTCGA GGAGAGATCA CGGTTTGGCT CCCCACGCAC TCCTCGCAAG 
AAGCGAGTGC GTCCAAAGAG ACGCCATCAG GCGGGCGATC GGCTGAGCGC TTCTGGTGGC 
GGGGTAAGCA ACGATCCCGA TAGCCTGGAT GAACTGGCCT ATGTGGACAC ACTGCCGGAG 
CAGGAAGTCA AGCTGGTCGA GGTGACCTCC AACATTTGGG GAACCAAGTT CAAGATCCAT 
GGACTTGCCA AAACCGTCCC AGCCAATTTA GGCCAAGTAA CTTATAAGAC GTCCCTACTG 
CATTTGCAGC CGCGTCAAAT GACGCTGGTC ATCACCGAGC TGCGCGACGA TTTTCCACCT 
GGTCCCGATC CCAGCTTTAA TCCGAACATA TTCTCTGAGG ACGAGGATGA GCATCACCAG 
CACCAGGGAA TACATCACGA TGCAGTGCCG CAGGTTAATG TGATCACCGC TCAGGATGGG 
GCTTCCTTAA AGCCGCCTAT AATACCGCAA CGTCGCCTCA CCGATGGAGC ATCTGCACCA 
TTGATAGCGC CCATGTCACC ACGTCCAAAT CGAATCCTGG CGCGACACAA GAACTCATTG 
ACTGTGAACG GAGAACGAGG ATCTGGCTCG TCGGCGGGCT TGAGTCCCCT AGCGCGAGCG 
GAGAGCTACG ATGATGATTC ATCCAATGAA TCCCAGGAGG CAGGAGCTGC AGGCAGCCAG 
TCAACCACTG TGCTACTGCA CCAGGCGCCC TCGAATGGCT CGGGTCCTGG ACCCAGCTGC 
AGTAAGAGCA TAACGCGTCC CAAAACTATC AGCAGCTTCA AAAACAGCTA CAGCCGCTCC 
AGCTCCAATT CCAGCTGCCA GTCGCGTCAC GCCATCTCGC CACTGTACTG CGATGGAGCA 
GTGCCCACGC TGCAGAGTCC CAAGAATGCA GTGGCTCCTT CGGACATCAT CTTCGAGCGT 
CCAGCAGTGC CCGCCGCTGG CCAGACCACC CTGATGTCCT ACTCGAGCAA TGCGGACTAT 
GCCAATAATG TGGTTCAGGT GAAGAATGCT CTGATGTCGG AGCCCGTGCG CTCGGCCAAC 
AGCCATGTGA ATCCAGTGCC GCTGAACCTC AATCTAAATC TGGAGCGAAT GGATGCACGG 
GTTAAGTGCA CGGCACCAAC CACATCTTCG ACGGCCAAGC GACGGGAAAT GTTGTATATC 
GATGAGGAGA CGCAGTCGCC AACGCCTACG CCAAGTAGCA GCAGCATGAA ACGAACGCCA 
ACGGTGGTTT CAATAGCTCC TGCTTTGCCC GATTCCATCA CACGCAGCTG CAGCGTCGGC 
TATCTGGATT CGGTTGCCAT CACGCCATCC GACGAAGCGC TGTCCGCTCT GCGTAAGGAT 
GCGCCAAACA AGCGGCTGAT ATTGGTGGAC AAGAAGAGGA ATCGCCGGAA AAGGCAACAG 
CAGGAGGATG CCCGGCGCCA CAAGCTGCAG CAAACTGGGA AGTCCAAGAG CCTAGACTCC 
TGCGACCTGT TGTCCCTACA GACTAAGCTG TCCAGCAAGG AGCACGAGCA GGTGGTGCGC 
AAGCTGCAGG AGATATCCGA TAGCAGCGCC TGCAGCAGTG CGGCCAACAC ATTGTGCTTC 
AAGTGCCGCA ACAACATGAG CCCCAGCAGT GCCTGCAAAC GGTGTCAGCC CTCGGCGAGC 
TCCGTCCTGG ATGAGATTAC AGCAGTCGTG CCCGCGGTGG AGCCGGCCAA GGAGTCTCCT 
GCGGTGCAGG CCAAGCCAGC GCCCAAGAAA CGCTTCGATG TTATCACTAG CTTCACAGAC 
TCGCCACTTT TTACGAGGAA ACATCGATTT GGAATCGGCC GCAGTAAGGA AACGGCTGGC 
ACTGAAAACT CCACTCCACT GCTGGGCAGA AAGCAGGACA ATGGCTTCAG CTTTGTGAAG 
CAGCTGTCCG AGGTGCGTTG GAGGCGCAAG GAACAGCCAG CACAGGCGCA AGTTAATGGT 
TCTAGTAATG CCAGTACTTT AGAGCGTCAG CCACAGCCGG AGATCACAGG TGCCGCTTGT 
GGCACAGTGG AAGCTACACC AGTGGAGGCG AAGGCTTCGG TTTCTCTGCA TACTCAGGCT 
CTCACCACTT TAGAGAACAT AATAAGCCGC CTTCGAGATC TAGACGAGGG TCGTTTGACG 
CCTCCTTCAA CCCCACAACG ACTGCCTCGA AGTTCTCCTG CATCTCCGGC AGCCAGCAAA 
AAGAACAAAA GGCAGCAGAG CAACTCTCCC ATTCGCCACA TATTGAATTC ACCGCTTTTG 
AATCGGCGTC AGCGCAAAAA ACAGAGCATT ATCGAGAGCT CCGATGATGA GGGTAACCAG 
ACCAATGGAT CCGGTGAGGA GAGCAACAAT GCGGGCAATG GCAAGCAGTA TCGCGACCTG 
GAGACCTTCC AAAAAGCTCA ACTGCGTCAG AAGCTAAAGC GCGGCAAGAT TGAACCCAAT 
GGCAGTGCTA GTTGTGCCAA TCCGGCGCCA GTGCGTCGTG AATTTGTGAT GCACAACAAG 
GCGCCCATGT GGAATGAGAT GAGTCAGGTG TACCAGCTGG ACTTTGGCGG ACGCGTTACC 
CAGGAGTCAG CCAAGAACTT CCAAATCGAG TTTCGTGGCA AGCAGGTCAT GCAATTCGGT 
CGCATTGATG GAAATGCCTA TACCCTGGAT TTCCAGTATC CTTTCTCCGC CCTCCAGGCC 
TTCGCCGTGG CTCTGGCCAA TGTGACACAG CGACTTAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tusp ( NM_001276086.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001263015.1 Drosophila melanogaster tusp, transcript variant G (Tusp), mRNA.

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CloneID	OFa08921
Clone ID Related Accession (Same CDS sequence)	NM_001276086.2
Accession Version	NM_001276086.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tusp, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001276086.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATTTAC ACTTTGAGCG AAACATCAAC ACAAAATGCG ACTGCACGAT ACTGTCATTA 
TCATGGATGG GAAAGGTGCC AGATGATATA CCCGAGGACG AGGGATGGAA GTTGAACCGC 
ACCAACTACT ACCAGGAGGG ATGGCTGGCC ACGGGCAATG TGCGCGGCAT TGTGGGCGTA 
ACCTTTACCA CCTCACATTG CCGCAAGAAC ATGGACTATC CGTTGAGGAC CAACTACAAC 
CTGCGCGGGC ATAGATCGGA TGTTATCCTG GTCAAGTGGA ATGAGCCGTA TCAAAAGCTA 
GCCTCCTGCG ATAGTTCGGG CATCATCTTT GTGTGGATCA AGTACGAGGG TCGCTGGTCC 
ATCGAGCTGA TCAACGATCG GAACACACCG GTGACCCACT TCTCCTGGTC ACACGATGGC 
CGCATGGCGC TGATCTGCTA CCAGGATGGC TTCGTTCTGG TAGGATCCGT GGCTGGGCAG 
CGATATTGGT CATCCATGCT CAATCTAGAG TCCACCATTA CCTGCGGCAT TTGGACGCCC 
GACGACCAGC AGGTGTACTT CGGCACAACC CAGGGTCAGG TTATTGTGAT GGATGTTCAC 
GGAGCGATGG TGTCGCAGGT TCAGCTGTCC AACGATGTGC CCATCACCTC GATGGCCTGG 
TCCTGCGAGA AGTTCAAGAT GGAGGAGGGC GAGGAGGCGG AGCCCGGTGT AACCAATGCG 
GCCAAGCGTT CCTTTGTCCT GGCAGTAAGC TTCCAGAATG GATATATCTA TTTGCTAAAG 
TCGTTTGACG ACGTCTCACC CGCACATATC AACACATGCC TTAATGGCGC CCTTGGCATG 
GTCATGGAGT GGAGCAACTC TCGCGAGCTG CTCGCCGTCG CGGGAACCCT GCGAACGGGA 
GTCGACGGAA CGAAGACAGA AGACATGGGC ACGCCCAGTT GCTACACGAA TCTGGTCAAG 
TTCTACACGG AAACGGGCAC CTGTCTGTAT CAGGCTCACA TTCCATGCAG CTCCGCCACC 
GTTTCGGCCA TCACCTGGGG CCACAACGAC AAGCGACTGT TCATCGCCAC GGGGACACAG 
GTTCACATCG CCTGGGTCTC CAGACGGGTG GCCTCCCTGC AGCTGCTGTG CCGCCTCGAG 
ATCCAGGCGA GCGTCGGATC CGAGTCGCTG CTGCCCCTGC TGCCGTTGCC TTCTAGGATT 
AAGTCTCTCA TTGGCAATCT GTTTGCTCAA ACGATAAGAT GCTGTGTACC TGACCTCAAG 
TCGCTGCGGG ATTTTGTTTC GCGACCACCG CTCTGCTCCA CTCGGCTGCA CTGCACCATG 
ATCCGGCACG ACGACGACTC CAATCTGAGC TCCGGCACAT GCTATACGCT GTATCTGGAG 
TATCTGGGCG GCTTGGTGCC GCTGCTCAAG GGCAAGCGCA CCTCCAAGAT TCGTCCTGAG 
TTTGTAATCT TCGATCCCCA AGTTAATGAT TCCCCCTTGT ACTTTCAATA CTCCGCCGAG 
GCCAAGAGCT CCTCGGGCTC CAGCCAGTCC ACCACCACGG GGAACAGTGG GCGCACCGAC 
TCATCAGACA GTGACTTCGA GGAGAGATCA CGGTTTGGCT CCCCACGCAC TCCTCGCAAG 
AAGCGAGTGC GTCCAAAGAG ACGCCATCAG GCGGGCGATC GGCTGAGCGC TTCTGGTGGC 
GGGGTAAGCA ACGATCCCGA TAGCCTGGAT GAACTGGCCT ATGTGGACAC ACTGCCGGAG 
GAAGTCAAGC TGGTCGAGGT GACCTCCAAC ATTTGGGGAA CCAAGTTCAA GATCCATGGA 
CTTGCCAAAA CCGTCCCAGC CAATTTAGGC CAAGTAACTT ATAAGACGTC CCTACTGCAT 
TTGCAGCCGC GTCAAATGAC GCTGGTCATC ACCGAGCTGC GCGACGATTT TCCACCTGGT 
CCCGATCCCA GCTTTAATCC GAACATATTC TCTGAGGACG AGGATGAGCA TCACCAGCAC 
CAGGGAATAC ATCACGATGC AGTGCCGCAG GTTAATGTGA TCACCGCTCA GGATGGGGCT 
TCCTTAAAGC CGCCTATAAT ACCGCAACGT CGCCTCACCG ATGGAGCATC TGCACCATTG 
ATAGCGCCCA TGTCACCACG TCCAAATCGA ATCCTGGCGC GACACAAGAA CTCATTGACT 
GTGAACGGAG AACGAGGATC TGGCTCGTCG GCGGGCTTGA GTCCCCTAGC GCGAGCGGAG 
AGCTACGATG ATGATTCATC CAATGAATCC CAGGAGGCAG GAGCTGCAGG CAGCCAGTCA 
ACCACTGTGC TACTGCACCA GGCGCCCTCG AATGGCTCGG GTCCTGGACC CAGCTGCAGT 
AAGAGCATAA CGCGTCCCAA AACTATCAGC AGCTTCAAAA ACAGCTACAG CCGCTCCAGC 
TCCAATTCCA GCTGCCAGTC GCGTCACGCC ATCTCGCCAC TGTACTGCGA TGGAGCAGTG 
CCCACGCTGC AGAGTCCCAA GAATGCAGTG GCTCCTTCGG ACATCATCTT CGAGCGTCCA 
GCAGTGCCCG CCGCTGGCCA GACCACCCTG ATGTCCTACT CGAGCAATGC GGACTATGCC 
AATAATGTGG TTCAGGTGAA GAATGCTCTG ATGTCGGAGC CCGTGCGCTC GGCCAACAGC 
CATGTGAATC CAGTGCCGCT GAACCTCAAT CTAAATCTGG AGCGAATGGA TGCACGGGTT 
AAGTGCACGG CACCAACCAC ATCTTCGACG GCCAAGCGAC GGGAAATGTT GTATATCGAT 
GAGGAGACGC AGTCGCCAAC GCCTACGCCA AGTAGCAGCA GCATGAAACG AACGCCAACG 
GTGGTTTCAA TAGCTCCTGC TTTGCCCGAT TCCATCACAC GCAGCTGCAG CGTCGGCTAT 
CTGGATTCGG TTGCCATCAC GCCATCCGAC GAAGCGCTGT CCGCTCTGCG TAAGGATGCG 
CCAAACAAGC GGCTGATATT GGTGGACAAG AAGAGGAATC GCCGGAAAAG GCAACAGCAG 
GAGGATGCCC GGCGCCACAA GCTGCAGCAA ACTGGGAAGT CCAAGAGCCT AGACTCCTGC 
GACCTGTTGT CCCTACAGAC TAAGCTGTCC AGCAAGGAGC ACGAGCAGGT GGTGCGCAAG 
CTGCAGGAGA TATCCGATAG CAGCGCCTGC AGCAGTGCGG CCAACACATT GTGCTTCAAG 
TGCCGCAACA ACATGAGCCC CAGCAGTGCC TGCAAACGGT GTCAGCCCTC GGCGAGCTCC 
GTCCTGGATG AGATTACAGC AGTCGTGCCC GCGGTGGAGC CGGCCAAGGA GTCTCCTGCG 
GTGCAGGCCA AGCCAGCGCC CAAGAAACGC TTCGATGTTA TCACTAGCTT CACAGACTCG 
CCACTTTTTA CGAGGAAACA TCGATTTGGA ATCGGCCGCA GTAAGGAAAC GGCTGGCACT 
GAAAACTCCA CTCCACTGCT GGGCAGAAAG CAGGACAATG GCTTCAGCTT TGTGAAGCAG 
CTGTCCGAGG TGCGTTGGAG GCGCAAGGAA CAGCCAGCAC AGGCGCAAGT TAATGGTTCT 
AGTAATGCCA GTACTTTAGA GCGTCAGCCA CAGCCGGAGA TCACAGGTGC CGCTTGTGGC 
ACAGTGGAAG CTACACCAGT GGAGGCGAAG GCTTCGGTTT CTCTGCATAC TCAGGCTCTC 
ACCACTTTAG AGAACATAAT AAGCCGCCTT CGAGATCTAG ACGAGGGTCG TTTGACGCCT 
CCTTCAACCC CACAACGACT GCCTCGAAGT TCTCCTGCAT CTCCGGCAGC CAGCAAAAAG 
AACAAAAGGC AGCAGAGCAA CTCTCCCATT CGCCACATAT TGAATTCACC GCTTTTGAAT 
CGGCGTCAGC GCAAAAAACA GAGCATTATC GAGAGCTCCG ATGATGAGGG TAACCAGACC 
AATGGATCCG GTGAGGAGAG CAACAATGCG GGCAATGGCA AGCAGTATCG CGACCTGGAG 
ACCTTCCAAA AAGCTCAACT GCGTCAGAAG CGCGGCAAGA TTGAACCCAA TGGCAGTGCT 
AGTTGTGCCA ATCCGGCGCC AGTGCGTCGT GAATTTGTGA TGCACAACAA GGCGCCCATG 
TGGAATGAGA TGAGTCAGGT GTACCAGCTG GACTTTGGCG GACGCGTTAC CCAGGAGTCA 
GCCAAGAACT TCCAAATCGA GTTTCGTGGC AAGCAGGTCA TGCAATTCGG TCGCATTGAT 
GGAAATGCCT ATACCCTGGA TTTCCAGTAT CCTTTCTCCG CCCTCCAGGC CTTCGCCGTG 
GCTCTGGCCA ATGTGACACA GCGACTTAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263015.1
CDS	428..4720
Translation	MHLHFERNINTKCDCTILSLSWMGKVPDDIPEDEGWKLNRTNYYQEGWLATGNVRGIVGVTFTTSHCRKNMDYPLRTNYNLRGHRSDVILVKWNEPYQKLASCDSSGIIFVWIKYEGRWSIELINDRNTPVTHFSWSHDGRMALICYQDGFVLVGSVAGQRYWSSMLNLESTITCGIWTPDDQQVYFGTTQGQVIVMDVHGAMVSQVQLSNDVPITSMAWSCEKFKMEEGEEAEPGVTNAAKRSFVLAVSFQNGYIYLLKSFDDVSPAHINTCLNGALGMVMEWSNSRELLAVAGTLRTGVDGTKTEDMGTPSCYTNLVKFYTETGTCLYQAHIPCSSATVSAITWGHNDKRLFIATGTQVHIAWVSRRVASLQLLCRLEIQASVGSESLLPLLPLPSRIKSLIGNLFAQTIRCCVPDLKSLRDFVSRPPLCSTRLHCTMIRHDDDSNLSSGTCYTLYLEYLGGLVPLLKGKRTSKIRPEFVIFDPQVNDSPLYFQYSAEAKSSSGSSQSTTTGNSGRTDSSDSDFEERSRFGSPRTPRKKRVRPKRRHQAGDRLSASGGGVSNDPDSLDELAYVDTLPEEVKLVEVTSNIWGTKFKIHGLAKTVPANLGQVTYKTSLLHLQPRQMTLVITELRDDFPPGPDPSFNPNIFSEDEDEHHQHQGIHHDAVPQVNVITAQDGASLKPPIIPQRRLTDGASAPLIAPMSPRPNRILARHKNSLTVNGERGSGSSAGLSPLARAESYDDDSSNESQEAGAAGSQSTTVLLHQAPSNGSGPGPSCSKSITRPKTISSFKNSYSRSSSNSSCQSRHAISPLYCDGAVPTLQSPKNAVAPSDIIFERPAVPAAGQTTLMSYSSNADYANNVVQVKNALMSEPVRSANSHVNPVPLNLNLNLERMDARVKCTAPTTSSTAKRREMLYIDEETQSPTPTPSSSSMKRTPTVVSIAPALPDSITRSCSVGYLDSVAITPSDEALSALRKDAPNKRLILVDKKRNRRKRQQQEDARRHKLQQTGKSKSLDSCDLLSLQTKLSSKEHEQVVRKLQEISDSSACSSAANTLCFKCRNNMSPSSACKRCQPSASSVLDEITAVVPAVEPAKESPAVQAKPAPKKRFDVITSFTDSPLFTRKHRFGIGRSKETAGTENSTPLLGRKQDNGFSFVKQLSEVRWRRKEQPAQAQVNGSSNASTLERQPQPEITGAACGTVEATPVEAKASVSLHTQALTTLENIISRLRDLDEGRLTPPSTPQRLPRSSPASPAASKKNKRQQSNSPIRHILNSPLLNRRQRKKQSIIESSDDEGNQTNGSGEESNNAGNGKQYRDLETFQKAQLRQKRGKIEPNGSASCANPAPVRREFVMHNKAPMWNEMSQVYQLDFGGRVTQESAKNFQIEFRGKQVMQFGRIDGNAYTLDFQYPFSALQAFAVALANVTQRLK

Target ORF information:

RefSeq Version	NM_001276086.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tusp, transcript variant G (Tusp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276086.2

ORF Insert Sequence:

ATGCATTTAC ACTTTGAGCG AAACATCAAC ACAAAATGCG ACTGCACGAT ACTGTCATTA 
TCATGGATGG GAAAGGTGCC AGATGATATA CCCGAGGACG AGGGATGGAA GTTGAACCGC 
ACCAACTACT ACCAGGAGGG ATGGCTGGCC ACGGGCAATG TGCGCGGCAT TGTGGGCGTA 
ACCTTTACCA CCTCACATTG CCGCAAGAAC ATGGACTATC CGTTGAGGAC CAACTACAAC 
CTGCGCGGGC ATAGATCGGA TGTTATCCTG GTCAAGTGGA ATGAGCCGTA TCAAAAGCTA 
GCCTCCTGCG ATAGTTCGGG CATCATCTTT GTGTGGATCA AGTACGAGGG TCGCTGGTCC 
ATCGAGCTGA TCAACGATCG GAACACACCG GTGACCCACT TCTCCTGGTC ACACGATGGC 
CGCATGGCGC TGATCTGCTA CCAGGATGGC TTCGTTCTGG TAGGATCCGT GGCTGGGCAG 
CGATATTGGT CATCCATGCT CAATCTAGAG TCCACCATTA CCTGCGGCAT TTGGACGCCC 
GACGACCAGC AGGTGTACTT CGGCACAACC CAGGGTCAGG TTATTGTGAT GGATGTTCAC 
GGAGCGATGG TGTCGCAGGT TCAGCTGTCC AACGATGTGC CCATCACCTC GATGGCCTGG 
TCCTGCGAGA AGTTCAAGAT GGAGGAGGGC GAGGAGGCGG AGCCCGGTGT AACCAATGCG 
GCCAAGCGTT CCTTTGTCCT GGCAGTAAGC TTCCAGAATG GATATATCTA TTTGCTAAAG 
TCGTTTGACG ACGTCTCACC CGCACATATC AACACATGCC TTAATGGCGC CCTTGGCATG 
GTCATGGAGT GGAGCAACTC TCGCGAGCTG CTCGCCGTCG CGGGAACCCT GCGAACGGGA 
GTCGACGGAA CGAAGACAGA AGACATGGGC ACGCCCAGTT GCTACACGAA TCTGGTCAAG 
TTCTACACGG AAACGGGCAC CTGTCTGTAT CAGGCTCACA TTCCATGCAG CTCCGCCACC 
GTTTCGGCCA TCACCTGGGG CCACAACGAC AAGCGACTGT TCATCGCCAC GGGGACACAG 
GTTCACATCG CCTGGGTCTC CAGACGGGTG GCCTCCCTGC AGCTGCTGTG CCGCCTCGAG 
ATCCAGGCGA GCGTCGGATC CGAGTCGCTG CTGCCCCTGC TGCCGTTGCC TTCTAGGATT 
AAGTCTCTCA TTGGCAATCT GTTTGCTCAA ACGATAAGAT GCTGTGTACC TGACCTCAAG 
TCGCTGCGGG ATTTTGTTTC GCGACCACCG CTCTGCTCCA CTCGGCTGCA CTGCACCATG 
ATCCGGCACG ACGACGACTC CAATCTGAGC TCCGGCACAT GCTATACGCT GTATCTGGAG 
TATCTGGGCG GCTTGGTGCC GCTGCTCAAG GGCAAGCGCA CCTCCAAGAT TCGTCCTGAG 
TTTGTAATCT TCGATCCCCA AGTTAATGAT TCCCCCTTGT ACTTTCAATA CTCCGCCGAG 
GCCAAGAGCT CCTCGGGCTC CAGCCAGTCC ACCACCACGG GGAACAGTGG GCGCACCGAC 
TCATCAGACA GTGACTTCGA GGAGAGATCA CGGTTTGGCT CCCCACGCAC TCCTCGCAAG 
AAGCGAGTGC GTCCAAAGAG ACGCCATCAG GCGGGCGATC GGCTGAGCGC TTCTGGTGGC 
GGGGTAAGCA ACGATCCCGA TAGCCTGGAT GAACTGGCCT ATGTGGACAC ACTGCCGGAG 
GAAGTCAAGC TGGTCGAGGT GACCTCCAAC ATTTGGGGAA CCAAGTTCAA GATCCATGGA 
CTTGCCAAAA CCGTCCCAGC CAATTTAGGC CAAGTAACTT ATAAGACGTC CCTACTGCAT 
TTGCAGCCGC GTCAAATGAC GCTGGTCATC ACCGAGCTGC GCGACGATTT TCCACCTGGT 
CCCGATCCCA GCTTTAATCC GAACATATTC TCTGAGGACG AGGATGAGCA TCACCAGCAC 
CAGGGAATAC ATCACGATGC AGTGCCGCAG GTTAATGTGA TCACCGCTCA GGATGGGGCT 
TCCTTAAAGC CGCCTATAAT ACCGCAACGT CGCCTCACCG ATGGAGCATC TGCACCATTG 
ATAGCGCCCA TGTCACCACG TCCAAATCGA ATCCTGGCGC GACACAAGAA CTCATTGACT 
GTGAACGGAG AACGAGGATC TGGCTCGTCG GCGGGCTTGA GTCCCCTAGC GCGAGCGGAG 
AGCTACGATG ATGATTCATC CAATGAATCC CAGGAGGCAG GAGCTGCAGG CAGCCAGTCA 
ACCACTGTGC TACTGCACCA GGCGCCCTCG AATGGCTCGG GTCCTGGACC CAGCTGCAGT 
AAGAGCATAA CGCGTCCCAA AACTATCAGC AGCTTCAAAA ACAGCTACAG CCGCTCCAGC 
TCCAATTCCA GCTGCCAGTC GCGTCACGCC ATCTCGCCAC TGTACTGCGA TGGAGCAGTG 
CCCACGCTGC AGAGTCCCAA GAATGCAGTG GCTCCTTCGG ACATCATCTT CGAGCGTCCA 
GCAGTGCCCG CCGCTGGCCA GACCACCCTG ATGTCCTACT CGAGCAATGC GGACTATGCC 
AATAATGTGG TTCAGGTGAA GAATGCTCTG ATGTCGGAGC CCGTGCGCTC GGCCAACAGC 
CATGTGAATC CAGTGCCGCT GAACCTCAAT CTAAATCTGG AGCGAATGGA TGCACGGGTT 
AAGTGCACGG CACCAACCAC ATCTTCGACG GCCAAGCGAC GGGAAATGTT GTATATCGAT 
GAGGAGACGC AGTCGCCAAC GCCTACGCCA AGTAGCAGCA GCATGAAACG AACGCCAACG 
GTGGTTTCAA TAGCTCCTGC TTTGCCCGAT TCCATCACAC GCAGCTGCAG CGTCGGCTAT 
CTGGATTCGG TTGCCATCAC GCCATCCGAC GAAGCGCTGT CCGCTCTGCG TAAGGATGCG 
CCAAACAAGC GGCTGATATT GGTGGACAAG AAGAGGAATC GCCGGAAAAG GCAACAGCAG 
GAGGATGCCC GGCGCCACAA GCTGCAGCAA ACTGGGAAGT CCAAGAGCCT AGACTCCTGC 
GACCTGTTGT CCCTACAGAC TAAGCTGTCC AGCAAGGAGC ACGAGCAGGT GGTGCGCAAG 
CTGCAGGAGA TATCCGATAG CAGCGCCTGC AGCAGTGCGG CCAACACATT GTGCTTCAAG 
TGCCGCAACA ACATGAGCCC CAGCAGTGCC TGCAAACGGT GTCAGCCCTC GGCGAGCTCC 
GTCCTGGATG AGATTACAGC AGTCGTGCCC GCGGTGGAGC CGGCCAAGGA GTCTCCTGCG 
GTGCAGGCCA AGCCAGCGCC CAAGAAACGC TTCGATGTTA TCACTAGCTT CACAGACTCG 
CCACTTTTTA CGAGGAAACA TCGATTTGGA ATCGGCCGCA GTAAGGAAAC GGCTGGCACT 
GAAAACTCCA CTCCACTGCT GGGCAGAAAG CAGGACAATG GCTTCAGCTT TGTGAAGCAG 
CTGTCCGAGG TGCGTTGGAG GCGCAAGGAA CAGCCAGCAC AGGCGCAAGT TAATGGTTCT 
AGTAATGCCA GTACTTTAGA GCGTCAGCCA CAGCCGGAGA TCACAGGTGC CGCTTGTGGC 
ACAGTGGAAG CTACACCAGT GGAGGCGAAG GCTTCGGTTT CTCTGCATAC TCAGGCTCTC 
ACCACTTTAG AGAACATAAT AAGCCGCCTT CGAGATCTAG ACGAGGGTCG TTTGACGCCT 
CCTTCAACCC CACAACGACT GCCTCGAAGT TCTCCTGCAT CTCCGGCAGC CAGCAAAAAG 
AACAAAAGGC AGCAGAGCAA CTCTCCCATT CGCCACATAT TGAATTCACC GCTTTTGAAT 
CGGCGTCAGC GCAAAAAACA GAGCATTATC GAGAGCTCCG ATGATGAGGG TAACCAGACC 
AATGGATCCG GTGAGGAGAG CAACAATGCG GGCAATGGCA AGCAGTATCG CGACCTGGAG 
ACCTTCCAAA AAGCTCAACT GCGTCAGAAG CGCGGCAAGA TTGAACCCAA TGGCAGTGCT 
AGTTGTGCCA ATCCGGCGCC AGTGCGTCGT GAATTTGTGA TGCACAACAA GGCGCCCATG 
TGGAATGAGA TGAGTCAGGT GTACCAGCTG GACTTTGGCG GACGCGTTAC CCAGGAGTCA 
GCCAAGAACT TCCAAATCGA GTTTCGTGGC AAGCAGGTCA TGCAATTCGG TCGCATTGAT 
GGAAATGCCT ATACCCTGGA TTTCCAGTAT CCTTTCTCCG CCCTCCAGGC CTTCGCCGTG 
GCTCTGGCCA ATGTGACACA GCGACTTAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Tusp cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Clones

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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