Tl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tl
Entrez Gene ID	43222
Full Name	Toll
Synonyms	CG5490,CT17414,Dmel\CG5490,EP(3)1051,EP1051,Fs(1)Tl,Fs(3)Tl,T1,TL,TOL,Toll-1,Toll1,dToll,dToll1,mat(3)9,mel(3)10,mel(3)9,tl,toll,toll-1
General protein information	Preferred Names Toll Names CG5490 gene product from transcript CG5490-RB CG5490-PB CG5490-PC CG5490-PD EP1051 Tl-PB Tl-PC Tl-PD Toll-1 protein toll precursor toll toll receptor
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 97D2-97D2\|3-91 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_079794.3	NP_524518.1	toll, isoform B
NM_001276066.2	NP_001262995.1	toll, isoform C
NM_170287.3	NP_733166.1	toll, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results, not only revealed a novel function and modulation pattern of miR-958, but also provided a new insight into the underlying molecular mechanisms of Toll signaling in regulation of innate immunity.
Title: miR-958 inhibits Toll signaling and Drosomycin expression via direct targeting of Toll and Dif in Drosophila melanogaster.

WntD accumulates until the Toll gradient narrows to its desired spread
Title: A WntD-Dependent Integral Feedback Loop Attenuates Variability in Drosophila Toll Signaling.

These findings highlight a role for Sherpa in Drosophila host defense and suggest the SUMOylation-mediated regulation of dMyd88 functions in Toll innate immune signaling.
Title: Genome-wide RNAi screening implicates the E3 ubiquitin ligase Sherpa in mediating innate immune signaling by Toll in Drosophila adults.

Senju-mediated galactosylation suppresses undesirable Toll signaling activation during the steady state; however, Toll activation in response to infection leads to degalactosylation
Title: Dynamic regulation of innate immune responses in Drosophila by Senju-mediated glycosylation.

The Toll-dorsal pathway is required for resistance to viral oral infection in Drosophila.
Title: The Toll-dorsal pathway is required for resistance to viral oral infection in Drosophila.

The following Tl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06237	NM_170287.3 Latest version!	Drosophila melanogaster toll, transcript variant D (Tl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa06237	NM_079794.3 Latest version!	Drosophila melanogaster toll, transcript variant B (Tl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa08858	NM_001276066.2 Latest version!	Drosophila melanogaster toll, transcript variant C (Tl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tl ( NM_079794.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524518.1 Drosophila melanogaster toll, transcript variant B (Tl), mRNA.

Price & Availability↑

CloneID	OFa06237
Clone ID Related Accession (Same CDS sequence)	NM_079794.3 , NM_170287.3
Accession Version	NM_079794.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	toll, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_079794.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTCGAC TAAAGGCCGC TTCCGAGCTG GCATTGCTTG TGATCATCCT GCAACTGCTA 
CAGTGGCCAG GATCTGAAGC ATCCTTCGGT CGGGATGCGT GCAGTGAGAT GAGCATAGAC 
GGACTGTGTC AGTGTGCACC CATAATGTCG GAATACGAAA TCATCTGCCC GGCGAATGCG 
GAGAATCCCA CGTTTAGGCT AACCATCCAG CCCAAAGACT ACGTGCAGAT CATGTGCAAT 
CTGACGGATA CCACGGACTA CCAGCAGCTG CCGAAAAAGT TGCGGATCGG TGAGGTCGAC 
AGGGTTCAGA TGCGACGGTG TATGCTGCCC GGTCACACGC CGATAGCCAG CATCCTGGAC 
TATTTGGGCA TAGTGTCGCC CACGACGCTG ATCTTTGAGA GCGACAACTT GGGCATGAAC 
ATAACACGGC AGCACTTGGA CCGTTTGCAC GGTCTAAAGC GATTCCGGTT TACCACTCGC 
CGTCTCACAC ACATTCCCGC CAACCTACTG ACGGATATGC GGAATCTGAG TCATCTCGAA 
CTAAGGGCAA ATATCGAGGA AATGCCATCG CATTTGTTCG ACGATCTGGA GAACCTGGAG 
TCAATTGAAT TCGGTAGCAA TAAGCTGAGG CAAATGCCGC GTGGCATTTT CGGCAAGATG 
CCCAAGCTGA AGCAGCTCAA TTTGTGGAGC AATCAACTGC ACAACCTCAC CAAACACGAC 
TTCGAAGGTG CCACCTCGGT GCTGGGCATA GATATTCACG ATAATGGCAT CGAACAGTTG 
CCGCACGACG TCTTTGCCCA TCTCACGAAT GTCACGGATA TCAATCTGAG CGCCAATCTC 
TTCCGTTCCC TGCCGCAGGG CCTATTCGAC CACAACAAAC ACCTGAACGA GGTGCGTCTG 
ATGAACAATC GAGTGCCCCT GGCCACGCTG CCTTCGCGTC TGTTTGCTAA TCAGCCGGAA 
CTGCAGATCT TACGGCTGAG AGCTGAACTG CAATCGTTGC CGGGTGACTT ATTCGAGCAT 
TCCACACAGA TCACAAACAT CTCGTTGGGA GACAATCTGC TGAAAACTCT GCCCGCCACC 
TTGCTGGAAC ATCAGGTGAA CCTGCTGAGC TTGGACTTGA GCAACAACCG GCTGACACAT 
CTGCCGGATT CACTCTTTGC ACATACCACC AATCTGACGG ATCTGCGTCT GGAGGATAAT 
TTGCTAACCG GCATTAGCGG CGATATATTC AGCAACTTGG GCAACCTTGT GACCCTCGTC 
ATGAGCAGGA ATCGACTGCG TACCATCGAT TCCAGAGCCT TTGTAAGCAC AAATGGACTG 
CGACATTTGC ATTTGGATCA CAACGACATT GATCTGCAAC AGCCATTGCT GGACATCATG 
CTCCAGACCC AGATCAACTC CCCGTTTGGT TACATGCACG GCCTTTTGAC CCTTAATCTA 
CGCAACAACT CAATCATATT CGTCTACAAC GACTGGAAGA ACACGATGCT CCAGTTGAGG 
GAGCTGGACT TGAGTTACAA CAACATTAGC TCGCTGGGCT ACGAGGATCT GGCGTTCCTG 
TCTCAGAATA GATTGCACGT AAACATGACG CACAATAAGA TACGGAGGAT AGCGCTGCCC 
GAGGATGTGC ACTTGGGCGA GGGCTACAAC AATAATCTGG TGCATGTGGA TCTCAACGAT 
AATCCCCTGG TCTGCGACTG TACGATCCTC TGGTTTATTC AGCTGGTGAG GGGCGTCCAT 
AAGCCGCAGT ATTCCAGGCA GTTCAAATTA CGCACAGACC GATTGGTTTG CAGCCAGCCA 
AATGTCTTGG AGGGTACTCC AGTTCGTCAG ATAGAACCAC AGACGCTCAT CTGTCCGCTG 
GACTTCTCCG ATGATCCACG GGAAAGGAAG TGTCCGCGCG GCTGCAACTG CCATGTGCGA 
ACCTACGACA AAGCACTGGT AATCAACTGC CATAGTGGCA ATCTGACACA TGTTCCTCGC 
TTGCCCAATC TGCATAAGAA TATGCAATTA ATGGAACTTC ACTTGGAGAA CAACACACTG 
CTCAGGCTTC CTTCGGCCAA TACACCCGGA TATGAGAGTG TAACTAGTCT GCATCTGGCT 
GGCAACAATC TAACCAGCAT CGATGTGGAT CAACTGCCTA CCAATCTCAC CCATTTGGAT 
ATAAGTTGGA ACCACCTGCA GATGCTGAAC GCAACCGTGC TGGGTTTCCT TAATCGTACC 
ATGAAGTGGC GCTCGGTGAA GCTGTCCGGG AATCCGTGGA TGTGTGATTG TACTGCCAAG 
CCGCTACTCC TCTTCACCCA GGACAATTTC GAAAGGATTG GAGATCGCAA TGAAATGATG 
TGCGTGAACG CCGAGATGCC AACACGAATG GTGGAACTGT CGACCAACGA CATTTGTCCG 
GCGGAAAAGG GCGTGTTCAT AGCCCTTGCT GTGGTGATTG CCTTGACTGG TCTTTTGGCC 
GGTTTCACAG CGGCACTGTA CTACAAATTC CAGACGGAGA TCAAGATTTG GCTGTATGCT 
CATAATCTCC TCCTGTGGTT TGTCACGGAG GAGGATCTGG ACAAGGACAA GAAGTTCGAT 
GCCTTCATCT CGTACTCGCA CAAGGACCAG AGCTTCATTG AGGACTACTT GGTGCCGCAA 
CTGGAGCACG GTCCTCAGAA GTTCCAGCTC TGTGTGCACG AACGAGACTG GCTGGTAGGC 
GGACACATTC CAGAGAACAT TATGCGCTCT GTGGCGGATT CGAGACGAAC GATTATTGTG 
CTCAGCCAGA ACTTTATCAA ATCGGAGTGG GCACGCCTTG AATTCAGGGC GGCGCACAGA 
TCGGCACTGA ACGAAGGACG CTCGCGCATT ATTGTGATCA TATACTCGGA CATCGGAGAT 
GTGGAGAAGC TGGACGAAGA GCTGAAGGCC TACTTGAAGA TGAACACCTA CCTGAAGTGG 
GGCGACCCGT GGTTCTGGGA TAAGCTGCGA TTTGCCCTGC CACATCGCAG ACCCGTCGGC 
AATATCGGTA ATGGAGCGCT AATCAAGACG GCGCTGAAAG GTTCCACGGA CGACAAGTTG 
GAGTTGATCA AACCGTCGCC GGTGACGCCA CCGCTAACCA CGCCGCCGGC GGAGGCAACC 
AAAAATCCGC TGGTAGCGCA GCTAAATGGC GTCACCCCGC ATCAGGCCAT AATGATTGCC 
AACGGCAAGA ATGGTCTGAC GAATCTGTAC ACACCCAATG GCAAGTCGCA CGGTAATGGC 
CACATCAACG GCGCCTTCAT CATTAACACG AATGCCAAAC AGAGCGACGT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524518.1
CDS	580..3873
Translation	MSRLKAASELALLVIILQLLQWPGSEASFGRDACSEMSIDGLCQCAPIMSEYEIICPANAENPTFRLTIQPKDYVQIMCNLTDTTDYQQLPKKLRIGEVDRVQMRRCMLPGHTPIASILDYLGIVSPTTLIFESDNLGMNITRQHLDRLHGLKRFRFTTRRLTHIPANLLTDMRNLSHLELRANIEEMPSHLFDDLENLESIEFGSNKLRQMPRGIFGKMPKLKQLNLWSNQLHNLTKHDFEGATSVLGIDIHDNGIEQLPHDVFAHLTNVTDINLSANLFRSLPQGLFDHNKHLNEVRLMNNRVPLATLPSRLFANQPELQILRLRAELQSLPGDLFEHSTQITNISLGDNLLKTLPATLLEHQVNLLSLDLSNNRLTHLPDSLFAHTTNLTDLRLEDNLLTGISGDIFSNLGNLVTLVMSRNRLRTIDSRAFVSTNGLRHLHLDHNDIDLQQPLLDIMLQTQINSPFGYMHGLLTLNLRNNSIIFVYNDWKNTMLQLRELDLSYNNISSLGYEDLAFLSQNRLHVNMTHNKIRRIALPEDVHLGEGYNNNLVHVDLNDNPLVCDCTILWFIQLVRGVHKPQYSRQFKLRTDRLVCSQPNVLEGTPVRQIEPQTLICPLDFSDDPRERKCPRGCNCHVRTYDKALVINCHSGNLTHVPRLPNLHKNMQLMELHLENNTLLRLPSANTPGYESVTSLHLAGNNLTSIDVDQLPTNLTHLDISWNHLQMLNATVLGFLNRTMKWRSVKLSGNPWMCDCTAKPLLLFTQDNFERIGDRNEMMCVNAEMPTRMVELSTNDICPAEKGVFIALAVVIALTGLLAGFTAALYYKFQTEIKIWLYAHNLLLWFVTEEDLDKDKKFDAFISYSHKDQSFIEDYLVPQLEHGPQKFQLCVHERDWLVGGHIPENIMRSVADSRRTIIVLSQNFIKSEWARLEFRAAHRSALNEGRSRIIVIIYSDIGDVEKLDEELKAYLKMNTYLKWGDPWFWDKLRFALPHRRPVGNIGNGALIKTALKGSTDDKLELIKPSPVTPPLTTPPAEATKNPLVAQLNGVTPHQAIMIANGKNGLTNLYTPNGKSHGNGHINGAFIINTNAKQSDV

Target ORF information:

RefSeq Version	NM_079794.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster toll, transcript variant B (Tl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079794.3

ORF Insert Sequence:

ATGAGTCGAC TAAAGGCCGC TTCCGAGCTG GCATTGCTTG TGATCATCCT GCAACTGCTA 
CAGTGGCCAG GATCTGAAGC ATCCTTCGGT CGGGATGCGT GCAGTGAGAT GAGCATAGAC 
GGACTGTGTC AGTGTGCACC CATAATGTCG GAATACGAAA TCATCTGCCC GGCGAATGCG 
GAGAATCCCA CGTTTAGGCT AACCATCCAG CCCAAAGACT ACGTGCAGAT CATGTGCAAT 
CTGACGGATA CCACGGACTA CCAGCAGCTG CCGAAAAAGT TGCGGATCGG TGAGGTCGAC 
AGGGTTCAGA TGCGACGGTG TATGCTGCCC GGTCACACGC CGATAGCCAG CATCCTGGAC 
TATTTGGGCA TAGTGTCGCC CACGACGCTG ATCTTTGAGA GCGACAACTT GGGCATGAAC 
ATAACACGGC AGCACTTGGA CCGTTTGCAC GGTCTAAAGC GATTCCGGTT TACCACTCGC 
CGTCTCACAC ACATTCCCGC CAACCTACTG ACGGATATGC GGAATCTGAG TCATCTCGAA 
CTAAGGGCAA ATATCGAGGA AATGCCATCG CATTTGTTCG ACGATCTGGA GAACCTGGAG 
TCAATTGAAT TCGGTAGCAA TAAGCTGAGG CAAATGCCGC GTGGCATTTT CGGCAAGATG 
CCCAAGCTGA AGCAGCTCAA TTTGTGGAGC AATCAACTGC ACAACCTCAC CAAACACGAC 
TTCGAAGGTG CCACCTCGGT GCTGGGCATA GATATTCACG ATAATGGCAT CGAACAGTTG 
CCGCACGACG TCTTTGCCCA TCTCACGAAT GTCACGGATA TCAATCTGAG CGCCAATCTC 
TTCCGTTCCC TGCCGCAGGG CCTATTCGAC CACAACAAAC ACCTGAACGA GGTGCGTCTG 
ATGAACAATC GAGTGCCCCT GGCCACGCTG CCTTCGCGTC TGTTTGCTAA TCAGCCGGAA 
CTGCAGATCT TACGGCTGAG AGCTGAACTG CAATCGTTGC CGGGTGACTT ATTCGAGCAT 
TCCACACAGA TCACAAACAT CTCGTTGGGA GACAATCTGC TGAAAACTCT GCCCGCCACC 
TTGCTGGAAC ATCAGGTGAA CCTGCTGAGC TTGGACTTGA GCAACAACCG GCTGACACAT 
CTGCCGGATT CACTCTTTGC ACATACCACC AATCTGACGG ATCTGCGTCT GGAGGATAAT 
TTGCTAACCG GCATTAGCGG CGATATATTC AGCAACTTGG GCAACCTTGT GACCCTCGTC 
ATGAGCAGGA ATCGACTGCG TACCATCGAT TCCAGAGCCT TTGTAAGCAC AAATGGACTG 
CGACATTTGC ATTTGGATCA CAACGACATT GATCTGCAAC AGCCATTGCT GGACATCATG 
CTCCAGACCC AGATCAACTC CCCGTTTGGT TACATGCACG GCCTTTTGAC CCTTAATCTA 
CGCAACAACT CAATCATATT CGTCTACAAC GACTGGAAGA ACACGATGCT CCAGTTGAGG 
GAGCTGGACT TGAGTTACAA CAACATTAGC TCGCTGGGCT ACGAGGATCT GGCGTTCCTG 
TCTCAGAATA GATTGCACGT AAACATGACG CACAATAAGA TACGGAGGAT AGCGCTGCCC 
GAGGATGTGC ACTTGGGCGA GGGCTACAAC AATAATCTGG TGCATGTGGA TCTCAACGAT 
AATCCCCTGG TCTGCGACTG TACGATCCTC TGGTTTATTC AGCTGGTGAG GGGCGTCCAT 
AAGCCGCAGT ATTCCAGGCA GTTCAAATTA CGCACAGACC GATTGGTTTG CAGCCAGCCA 
AATGTCTTGG AGGGTACTCC AGTTCGTCAG ATAGAACCAC AGACGCTCAT CTGTCCGCTG 
GACTTCTCCG ATGATCCACG GGAAAGGAAG TGTCCGCGCG GCTGCAACTG CCATGTGCGA 
ACCTACGACA AAGCACTGGT AATCAACTGC CATAGTGGCA ATCTGACACA TGTTCCTCGC 
TTGCCCAATC TGCATAAGAA TATGCAATTA ATGGAACTTC ACTTGGAGAA CAACACACTG 
CTCAGGCTTC CTTCGGCCAA TACACCCGGA TATGAGAGTG TAACTAGTCT GCATCTGGCT 
GGCAACAATC TAACCAGCAT CGATGTGGAT CAACTGCCTA CCAATCTCAC CCATTTGGAT 
ATAAGTTGGA ACCACCTGCA GATGCTGAAC GCAACCGTGC TGGGTTTCCT TAATCGTACC 
ATGAAGTGGC GCTCGGTGAA GCTGTCCGGG AATCCGTGGA TGTGTGATTG TACTGCCAAG 
CCGCTACTCC TCTTCACCCA GGACAATTTC GAAAGGATTG GAGATCGCAA TGAAATGATG 
TGCGTGAACG CCGAGATGCC AACACGAATG GTGGAACTGT CGACCAACGA CATTTGTCCG 
GCGGAAAAGG GCGTGTTCAT AGCCCTTGCT GTGGTGATTG CCTTGACTGG TCTTTTGGCC 
GGTTTCACAG CGGCACTGTA CTACAAATTC CAGACGGAGA TCAAGATTTG GCTGTATGCT 
CATAATCTCC TCCTGTGGTT TGTCACGGAG GAGGATCTGG ACAAGGACAA GAAGTTCGAT 
GCCTTCATCT CGTACTCGCA CAAGGACCAG AGCTTCATTG AGGACTACTT GGTGCCGCAA 
CTGGAGCACG GTCCTCAGAA GTTCCAGCTC TGTGTGCACG AACGAGACTG GCTGGTAGGC 
GGACACATTC CAGAGAACAT TATGCGCTCT GTGGCGGATT CGAGACGAAC GATTATTGTG 
CTCAGCCAGA ACTTTATCAA ATCGGAGTGG GCACGCCTTG AATTCAGGGC GGCGCACAGA 
TCGGCACTGA ACGAAGGACG CTCGCGCATT ATTGTGATCA TATACTCGGA CATCGGAGAT 
GTGGAGAAGC TGGACGAAGA GCTGAAGGCC TACTTGAAGA TGAACACCTA CCTGAAGTGG 
GGCGACCCGT GGTTCTGGGA TAAGCTGCGA TTTGCCCTGC CACATCGCAG ACCCGTCGGC 
AATATCGGTA ATGGAGCGCT AATCAAGACG GCGCTGAAAG GTTCCACGGA CGACAAGTTG 
GAGTTGATCA AACCGTCGCC GGTGACGCCA CCGCTAACCA CGCCGCCGGC GGAGGCAACC 
AAAAATCCGC TGGTAGCGCA GCTAAATGGC GTCACCCCGC ATCAGGCCAT AATGATTGCC 
AACGGCAAGA ATGGTCTGAC GAATCTGTAC ACACCCAATG GCAAGTCGCA CGGTAATGGC 
CACATCAACG GCGCCTTCAT CATTAACACG AATGCCAAAC AGAGCGACGT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tl ( NM_170287.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733166.1 Drosophila melanogaster toll, transcript variant D (Tl), mRNA.

Price & Availability↑

CloneID	OFa06237
Clone ID Related Accession (Same CDS sequence)	NM_079794.3 , NM_170287.3
Accession Version	NM_170287.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	toll, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_170287.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTCGAC TAAAGGCCGC TTCCGAGCTG GCATTGCTTG TGATCATCCT GCAACTGCTA 
CAGTGGCCAG GATCTGAAGC ATCCTTCGGT CGGGATGCGT GCAGTGAGAT GAGCATAGAC 
GGACTGTGTC AGTGTGCACC CATAATGTCG GAATACGAAA TCATCTGCCC GGCGAATGCG 
GAGAATCCCA CGTTTAGGCT AACCATCCAG CCCAAAGACT ACGTGCAGAT CATGTGCAAT 
CTGACGGATA CCACGGACTA CCAGCAGCTG CCGAAAAAGT TGCGGATCGG TGAGGTCGAC 
AGGGTTCAGA TGCGACGGTG TATGCTGCCC GGTCACACGC CGATAGCCAG CATCCTGGAC 
TATTTGGGCA TAGTGTCGCC CACGACGCTG ATCTTTGAGA GCGACAACTT GGGCATGAAC 
ATAACACGGC AGCACTTGGA CCGTTTGCAC GGTCTAAAGC GATTCCGGTT TACCACTCGC 
CGTCTCACAC ACATTCCCGC CAACCTACTG ACGGATATGC GGAATCTGAG TCATCTCGAA 
CTAAGGGCAA ATATCGAGGA AATGCCATCG CATTTGTTCG ACGATCTGGA GAACCTGGAG 
TCAATTGAAT TCGGTAGCAA TAAGCTGAGG CAAATGCCGC GTGGCATTTT CGGCAAGATG 
CCCAAGCTGA AGCAGCTCAA TTTGTGGAGC AATCAACTGC ACAACCTCAC CAAACACGAC 
TTCGAAGGTG CCACCTCGGT GCTGGGCATA GATATTCACG ATAATGGCAT CGAACAGTTG 
CCGCACGACG TCTTTGCCCA TCTCACGAAT GTCACGGATA TCAATCTGAG CGCCAATCTC 
TTCCGTTCCC TGCCGCAGGG CCTATTCGAC CACAACAAAC ACCTGAACGA GGTGCGTCTG 
ATGAACAATC GAGTGCCCCT GGCCACGCTG CCTTCGCGTC TGTTTGCTAA TCAGCCGGAA 
CTGCAGATCT TACGGCTGAG AGCTGAACTG CAATCGTTGC CGGGTGACTT ATTCGAGCAT 
TCCACACAGA TCACAAACAT CTCGTTGGGA GACAATCTGC TGAAAACTCT GCCCGCCACC 
TTGCTGGAAC ATCAGGTGAA CCTGCTGAGC TTGGACTTGA GCAACAACCG GCTGACACAT 
CTGCCGGATT CACTCTTTGC ACATACCACC AATCTGACGG ATCTGCGTCT GGAGGATAAT 
TTGCTAACCG GCATTAGCGG CGATATATTC AGCAACTTGG GCAACCTTGT GACCCTCGTC 
ATGAGCAGGA ATCGACTGCG TACCATCGAT TCCAGAGCCT TTGTAAGCAC AAATGGACTG 
CGACATTTGC ATTTGGATCA CAACGACATT GATCTGCAAC AGCCATTGCT GGACATCATG 
CTCCAGACCC AGATCAACTC CCCGTTTGGT TACATGCACG GCCTTTTGAC CCTTAATCTA 
CGCAACAACT CAATCATATT CGTCTACAAC GACTGGAAGA ACACGATGCT CCAGTTGAGG 
GAGCTGGACT TGAGTTACAA CAACATTAGC TCGCTGGGCT ACGAGGATCT GGCGTTCCTG 
TCTCAGAATA GATTGCACGT AAACATGACG CACAATAAGA TACGGAGGAT AGCGCTGCCC 
GAGGATGTGC ACTTGGGCGA GGGCTACAAC AATAATCTGG TGCATGTGGA TCTCAACGAT 
AATCCCCTGG TCTGCGACTG TACGATCCTC TGGTTTATTC AGCTGGTGAG GGGCGTCCAT 
AAGCCGCAGT ATTCCAGGCA GTTCAAATTA CGCACAGACC GATTGGTTTG CAGCCAGCCA 
AATGTCTTGG AGGGTACTCC AGTTCGTCAG ATAGAACCAC AGACGCTCAT CTGTCCGCTG 
GACTTCTCCG ATGATCCACG GGAAAGGAAG TGTCCGCGCG GCTGCAACTG CCATGTGCGA 
ACCTACGACA AAGCACTGGT AATCAACTGC CATAGTGGCA ATCTGACACA TGTTCCTCGC 
TTGCCCAATC TGCATAAGAA TATGCAATTA ATGGAACTTC ACTTGGAGAA CAACACACTG 
CTCAGGCTTC CTTCGGCCAA TACACCCGGA TATGAGAGTG TAACTAGTCT GCATCTGGCT 
GGCAACAATC TAACCAGCAT CGATGTGGAT CAACTGCCTA CCAATCTCAC CCATTTGGAT 
ATAAGTTGGA ACCACCTGCA GATGCTGAAC GCAACCGTGC TGGGTTTCCT TAATCGTACC 
ATGAAGTGGC GCTCGGTGAA GCTGTCCGGG AATCCGTGGA TGTGTGATTG TACTGCCAAG 
CCGCTACTCC TCTTCACCCA GGACAATTTC GAAAGGATTG GAGATCGCAA TGAAATGATG 
TGCGTGAACG CCGAGATGCC AACACGAATG GTGGAACTGT CGACCAACGA CATTTGTCCG 
GCGGAAAAGG GCGTGTTCAT AGCCCTTGCT GTGGTGATTG CCTTGACTGG TCTTTTGGCC 
GGTTTCACAG CGGCACTGTA CTACAAATTC CAGACGGAGA TCAAGATTTG GCTGTATGCT 
CATAATCTCC TCCTGTGGTT TGTCACGGAG GAGGATCTGG ACAAGGACAA GAAGTTCGAT 
GCCTTCATCT CGTACTCGCA CAAGGACCAG AGCTTCATTG AGGACTACTT GGTGCCGCAA 
CTGGAGCACG GTCCTCAGAA GTTCCAGCTC TGTGTGCACG AACGAGACTG GCTGGTAGGC 
GGACACATTC CAGAGAACAT TATGCGCTCT GTGGCGGATT CGAGACGAAC GATTATTGTG 
CTCAGCCAGA ACTTTATCAA ATCGGAGTGG GCACGCCTTG AATTCAGGGC GGCGCACAGA 
TCGGCACTGA ACGAAGGACG CTCGCGCATT ATTGTGATCA TATACTCGGA CATCGGAGAT 
GTGGAGAAGC TGGACGAAGA GCTGAAGGCC TACTTGAAGA TGAACACCTA CCTGAAGTGG 
GGCGACCCGT GGTTCTGGGA TAAGCTGCGA TTTGCCCTGC CACATCGCAG ACCCGTCGGC 
AATATCGGTA ATGGAGCGCT AATCAAGACG GCGCTGAAAG GTTCCACGGA CGACAAGTTG 
GAGTTGATCA AACCGTCGCC GGTGACGCCA CCGCTAACCA CGCCGCCGGC GGAGGCAACC 
AAAAATCCGC TGGTAGCGCA GCTAAATGGC GTCACCCCGC ATCAGGCCAT AATGATTGCC 
AACGGCAAGA ATGGTCTGAC GAATCTGTAC ACACCCAATG GCAAGTCGCA CGGTAATGGC 
CACATCAACG GCGCCTTCAT CATTAACACG AATGCCAAAC AGAGCGACGT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733166.1
CDS	649..3942
Translation	MSRLKAASELALLVIILQLLQWPGSEASFGRDACSEMSIDGLCQCAPIMSEYEIICPANAENPTFRLTIQPKDYVQIMCNLTDTTDYQQLPKKLRIGEVDRVQMRRCMLPGHTPIASILDYLGIVSPTTLIFESDNLGMNITRQHLDRLHGLKRFRFTTRRLTHIPANLLTDMRNLSHLELRANIEEMPSHLFDDLENLESIEFGSNKLRQMPRGIFGKMPKLKQLNLWSNQLHNLTKHDFEGATSVLGIDIHDNGIEQLPHDVFAHLTNVTDINLSANLFRSLPQGLFDHNKHLNEVRLMNNRVPLATLPSRLFANQPELQILRLRAELQSLPGDLFEHSTQITNISLGDNLLKTLPATLLEHQVNLLSLDLSNNRLTHLPDSLFAHTTNLTDLRLEDNLLTGISGDIFSNLGNLVTLVMSRNRLRTIDSRAFVSTNGLRHLHLDHNDIDLQQPLLDIMLQTQINSPFGYMHGLLTLNLRNNSIIFVYNDWKNTMLQLRELDLSYNNISSLGYEDLAFLSQNRLHVNMTHNKIRRIALPEDVHLGEGYNNNLVHVDLNDNPLVCDCTILWFIQLVRGVHKPQYSRQFKLRTDRLVCSQPNVLEGTPVRQIEPQTLICPLDFSDDPRERKCPRGCNCHVRTYDKALVINCHSGNLTHVPRLPNLHKNMQLMELHLENNTLLRLPSANTPGYESVTSLHLAGNNLTSIDVDQLPTNLTHLDISWNHLQMLNATVLGFLNRTMKWRSVKLSGNPWMCDCTAKPLLLFTQDNFERIGDRNEMMCVNAEMPTRMVELSTNDICPAEKGVFIALAVVIALTGLLAGFTAALYYKFQTEIKIWLYAHNLLLWFVTEEDLDKDKKFDAFISYSHKDQSFIEDYLVPQLEHGPQKFQLCVHERDWLVGGHIPENIMRSVADSRRTIIVLSQNFIKSEWARLEFRAAHRSALNEGRSRIIVIIYSDIGDVEKLDEELKAYLKMNTYLKWGDPWFWDKLRFALPHRRPVGNIGNGALIKTALKGSTDDKLELIKPSPVTPPLTTPPAEATKNPLVAQLNGVTPHQAIMIANGKNGLTNLYTPNGKSHGNGHINGAFIINTNAKQSDV

Target ORF information:

RefSeq Version	NM_170287.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster toll, transcript variant D (Tl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170287.3

ORF Insert Sequence:

ATGAGTCGAC TAAAGGCCGC TTCCGAGCTG GCATTGCTTG TGATCATCCT GCAACTGCTA 
CAGTGGCCAG GATCTGAAGC ATCCTTCGGT CGGGATGCGT GCAGTGAGAT GAGCATAGAC 
GGACTGTGTC AGTGTGCACC CATAATGTCG GAATACGAAA TCATCTGCCC GGCGAATGCG 
GAGAATCCCA CGTTTAGGCT AACCATCCAG CCCAAAGACT ACGTGCAGAT CATGTGCAAT 
CTGACGGATA CCACGGACTA CCAGCAGCTG CCGAAAAAGT TGCGGATCGG TGAGGTCGAC 
AGGGTTCAGA TGCGACGGTG TATGCTGCCC GGTCACACGC CGATAGCCAG CATCCTGGAC 
TATTTGGGCA TAGTGTCGCC CACGACGCTG ATCTTTGAGA GCGACAACTT GGGCATGAAC 
ATAACACGGC AGCACTTGGA CCGTTTGCAC GGTCTAAAGC GATTCCGGTT TACCACTCGC 
CGTCTCACAC ACATTCCCGC CAACCTACTG ACGGATATGC GGAATCTGAG TCATCTCGAA 
CTAAGGGCAA ATATCGAGGA AATGCCATCG CATTTGTTCG ACGATCTGGA GAACCTGGAG 
TCAATTGAAT TCGGTAGCAA TAAGCTGAGG CAAATGCCGC GTGGCATTTT CGGCAAGATG 
CCCAAGCTGA AGCAGCTCAA TTTGTGGAGC AATCAACTGC ACAACCTCAC CAAACACGAC 
TTCGAAGGTG CCACCTCGGT GCTGGGCATA GATATTCACG ATAATGGCAT CGAACAGTTG 
CCGCACGACG TCTTTGCCCA TCTCACGAAT GTCACGGATA TCAATCTGAG CGCCAATCTC 
TTCCGTTCCC TGCCGCAGGG CCTATTCGAC CACAACAAAC ACCTGAACGA GGTGCGTCTG 
ATGAACAATC GAGTGCCCCT GGCCACGCTG CCTTCGCGTC TGTTTGCTAA TCAGCCGGAA 
CTGCAGATCT TACGGCTGAG AGCTGAACTG CAATCGTTGC CGGGTGACTT ATTCGAGCAT 
TCCACACAGA TCACAAACAT CTCGTTGGGA GACAATCTGC TGAAAACTCT GCCCGCCACC 
TTGCTGGAAC ATCAGGTGAA CCTGCTGAGC TTGGACTTGA GCAACAACCG GCTGACACAT 
CTGCCGGATT CACTCTTTGC ACATACCACC AATCTGACGG ATCTGCGTCT GGAGGATAAT 
TTGCTAACCG GCATTAGCGG CGATATATTC AGCAACTTGG GCAACCTTGT GACCCTCGTC 
ATGAGCAGGA ATCGACTGCG TACCATCGAT TCCAGAGCCT TTGTAAGCAC AAATGGACTG 
CGACATTTGC ATTTGGATCA CAACGACATT GATCTGCAAC AGCCATTGCT GGACATCATG 
CTCCAGACCC AGATCAACTC CCCGTTTGGT TACATGCACG GCCTTTTGAC CCTTAATCTA 
CGCAACAACT CAATCATATT CGTCTACAAC GACTGGAAGA ACACGATGCT CCAGTTGAGG 
GAGCTGGACT TGAGTTACAA CAACATTAGC TCGCTGGGCT ACGAGGATCT GGCGTTCCTG 
TCTCAGAATA GATTGCACGT AAACATGACG CACAATAAGA TACGGAGGAT AGCGCTGCCC 
GAGGATGTGC ACTTGGGCGA GGGCTACAAC AATAATCTGG TGCATGTGGA TCTCAACGAT 
AATCCCCTGG TCTGCGACTG TACGATCCTC TGGTTTATTC AGCTGGTGAG GGGCGTCCAT 
AAGCCGCAGT ATTCCAGGCA GTTCAAATTA CGCACAGACC GATTGGTTTG CAGCCAGCCA 
AATGTCTTGG AGGGTACTCC AGTTCGTCAG ATAGAACCAC AGACGCTCAT CTGTCCGCTG 
GACTTCTCCG ATGATCCACG GGAAAGGAAG TGTCCGCGCG GCTGCAACTG CCATGTGCGA 
ACCTACGACA AAGCACTGGT AATCAACTGC CATAGTGGCA ATCTGACACA TGTTCCTCGC 
TTGCCCAATC TGCATAAGAA TATGCAATTA ATGGAACTTC ACTTGGAGAA CAACACACTG 
CTCAGGCTTC CTTCGGCCAA TACACCCGGA TATGAGAGTG TAACTAGTCT GCATCTGGCT 
GGCAACAATC TAACCAGCAT CGATGTGGAT CAACTGCCTA CCAATCTCAC CCATTTGGAT 
ATAAGTTGGA ACCACCTGCA GATGCTGAAC GCAACCGTGC TGGGTTTCCT TAATCGTACC 
ATGAAGTGGC GCTCGGTGAA GCTGTCCGGG AATCCGTGGA TGTGTGATTG TACTGCCAAG 
CCGCTACTCC TCTTCACCCA GGACAATTTC GAAAGGATTG GAGATCGCAA TGAAATGATG 
TGCGTGAACG CCGAGATGCC AACACGAATG GTGGAACTGT CGACCAACGA CATTTGTCCG 
GCGGAAAAGG GCGTGTTCAT AGCCCTTGCT GTGGTGATTG CCTTGACTGG TCTTTTGGCC 
GGTTTCACAG CGGCACTGTA CTACAAATTC CAGACGGAGA TCAAGATTTG GCTGTATGCT 
CATAATCTCC TCCTGTGGTT TGTCACGGAG GAGGATCTGG ACAAGGACAA GAAGTTCGAT 
GCCTTCATCT CGTACTCGCA CAAGGACCAG AGCTTCATTG AGGACTACTT GGTGCCGCAA 
CTGGAGCACG GTCCTCAGAA GTTCCAGCTC TGTGTGCACG AACGAGACTG GCTGGTAGGC 
GGACACATTC CAGAGAACAT TATGCGCTCT GTGGCGGATT CGAGACGAAC GATTATTGTG 
CTCAGCCAGA ACTTTATCAA ATCGGAGTGG GCACGCCTTG AATTCAGGGC GGCGCACAGA 
TCGGCACTGA ACGAAGGACG CTCGCGCATT ATTGTGATCA TATACTCGGA CATCGGAGAT 
GTGGAGAAGC TGGACGAAGA GCTGAAGGCC TACTTGAAGA TGAACACCTA CCTGAAGTGG 
GGCGACCCGT GGTTCTGGGA TAAGCTGCGA TTTGCCCTGC CACATCGCAG ACCCGTCGGC 
AATATCGGTA ATGGAGCGCT AATCAAGACG GCGCTGAAAG GTTCCACGGA CGACAAGTTG 
GAGTTGATCA AACCGTCGCC GGTGACGCCA CCGCTAACCA CGCCGCCGGC GGAGGCAACC 
AAAAATCCGC TGGTAGCGCA GCTAAATGGC GTCACCCCGC ATCAGGCCAT AATGATTGCC 
AACGGCAAGA ATGGTCTGAC GAATCTGTAC ACACCCAATG GCAAGTCGCA CGGTAATGGC 
CACATCAACG GCGCCTTCAT CATTAACACG AATGCCAAAC AGAGCGACGT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tl ( NM_001276066.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262995.1 Drosophila melanogaster toll, transcript variant C (Tl), mRNA.

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CloneID	OFa08858
Clone ID Related Accession (Same CDS sequence)	NM_001276066.2
Accession Version	NM_001276066.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	toll, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001276066.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTCGAC TAAAGGCCGC TTCCGAGCTG GCATTGCTTG TGATCATCCT GCAACTGCTA 
CAGTGGCCAG GATCTGAAGC ATCCTTCGGT CGGGATGCGT GCAGTGAGAT GAGCATAGAC 
GGACTGTGTC AGTGTGCACC CATAATGTCG GAATACGAAA TCATCTGCCC GGCGAATGCG 
GAGAATCCCA CGTTTAGGCT AACCATCCAG CCCAAAGACT ACGTGCAGAT CATGTGCAAT 
CTGACGGATA CCACGGACTA CCAGCAGCTG CCGAAAAAGT TGCGGATCGG TGAGGTCGAC 
AGGGTTCAGA TGCGACGGTG TATGCTGCCC GGTCACACGC CGATAGCCAG CATCCTGGAC 
TATTTGGGCA TAGTGTCGCC CACGACGCTG ATCTTTGAGA GCGACAACTT GGGCATGAAC 
ATAACACGGC AGCACTTGGA CCGTTTGCAC GGTCTAAAGC GATTCCGGTT TACCACTCGC 
CGTCTCACAC ACATTCCCGC CAACCTACTG ACGGATATGC GGAATCTGAG TCATCTCGAA 
CTAAGGGCAA ATATCGAGGA AATGCCATCG CATTTGTTCG ACGATCTGGA GAACCTGGAG 
TCAATTGAAT TCGGTAGCAA TAAGCTGAGG CAAATGCCGC GTGGCATTTT CGGCAAGATG 
CCCAAGCTGA AGCAGCTCAA TTTGTGGAGC AATCAACTGC ACAACCTCAC CAAACACGAC 
TTCGAAGGTG CCACCTCGGT GCTGGGCATA GATATTCACG ATAATGGCAT CGAACAGTTG 
CCGCACGACG TCTTTGCCCA TCTCACGAAT GTCACGGATA TCAATCTGAG CGCCAATCTC 
TTCCGTTCCC TGCCGCAGGG CCTATTCGAC CACAACAAAC ACCTGAACGA GGTGCGTCTG 
ATGAACAATC GAGTGCCCCT GGCCACGCTG CCTTCGCGTC TGTTTGCTAA TCAGCCGGAA 
CTGCAGATCT TACGGCTGAG AGCTGAACTG CAATCGTTGC CGGGTGACTT ATTCGAGCAT 
TCCACACAGA TCACAAACAT CTCGTTGGGA GACAATCTGC TGAAAACTCT GCCCGCCACC 
TTGCTGGAAC ATCAGGTGAA CCTGCTGAGC TTGGACTTGA GCAACAACCG GCTGACACAT 
CTGCCGGATT CACTCTTTGC ACATACCACC AATCTGACGG ATCTGCGTCT GGAGGATAAT 
TTGCTAACCG GCATTAGCGG CGATATATTC AGCAACTTGG GCAACCTTGT GACCCTCGTC 
ATGAGCAGGA ATCGACTGCG TACCATCGAT TCCAGAGCCT TTGTAAGCAC AAATGGACTG 
CGACATTTGC ATTTGGATCA CAACGACATT GATCTGCAAC AGCCATTGCT GGACATCATG 
CTCCAGACCC AGATCAACTC CCCGTTTGGT TACATGCACG GCCTTTTGAC CCTTAATCTA 
CGCAACAACT CAATCATATT CGTCTACAAC GACTGGAAGA ACACGATGCT CCAGTTGAGG 
GAGCTGGACT TGAGTTACAA CAACATTAGC TCGCTGGGCT ACGAGGATCT GGCGTTCCTG 
TCTCAGAATA GATTGCACGT AAACATGACG CACAATAAGA TACGGAGGAT AGCGCTGCCC 
GAGGATGTGC ACTTGGGCGA GGGCTACAAC AATAATCTGG TGCATGTGGA TCTCAACGAT 
AATCCCCTGG TCTGCGACTG TACGATCCTC TGGTTTATTC AGCTGGTGAG GGGCGTCCAT 
AAGCCGCAGT ATTCCAGGCA GTTCAAATTA CGCACAGACC GATTGGTTTG CAGCCAGCCA 
AATGTCTTGG AGGGTACTCC AGTTCGTCAG ATAGAACCAC AGACGCTCAT CTGTCCGCTG 
GACTTCTCCG ATGATCCACG GGAAAGGAAG TGTCCGCGCG GCTGCAACTG CCATGTGCGA 
ACCTACGACA AAGCACTGGT AATCAACTGC CATAGTGGCA ATCTGACACA TGTTCCTCGC 
TTGCCCAATC TGCATAAGAA TATGCAATTA ATGGAACTTC ACTTGGAGAA CAACACACTG 
CTCAGGCTTC CTTCGGCCAA TACACCCGGA TATGAGAGTG TAACTAGTCT GCATCTGGCT 
GGCAACAATC TAACCAGCAT CGATGTGGAT CAACTGCCTA CCAATCTCAC CCATTTGGAT 
ATAAGTTGGA ACCACCTGCA GATGCTGAAC GCAACCGTGC TGGGTTTCCT TAATCGTACC 
ATGAAGTGGC GCTCGGTGAA GCTGTCCGGG AATCCGTGGA TGTGTGATTG TACTGCCAAG 
CCGCTACTCC TCTTCACCCA GGACAATTTC GAAAGGATTG GAGATCGCAA TGAAATGATG 
TGCGTGAACG CCGAGATGCC AACACGAATG GTGGAACTGT CGACCAACGA CATTTGTCCG 
GCGGAAAAGG GCGTGTTCAT AGCCCTTGCT GTGGTGATTG CCTTGACTGG TCTTTTGGCC 
GGTTTCACAG CGGCACTGTA CTACAAATTC CAGACGGAGA TCAAGATTTG GCTGTATGCT 
CATAATCTCC TCCTGTGGTT TGTCACGGAG GAGGATCTGG ACAAGGACAA GAAGTTCGAT 
GCCTTCATCT CGTACTCGCA CAAGGACCAG AGCTTCATTG AGGACTACTT GGTGCCGCAA 
CTGGAGCACG GTCCTCAGAA GTTCCAGCTC TGTGTGCACG AACGAGACTG GCTGGTAGGC 
GGACACATTC CAGAGAACAT TATGCGCTCT GTGGCGGATT CGAGACGAAC GATTATTGTG 
CTCAGCCAGA ACTTTATCAA ATCGGAGTGG GCACGCCTTG AATTCAGGGC GGCGCACAGA 
TCGGCACTGA ACGAAGGACG CTCGCGCATT ATTGTGATCA TATACTCGGA CATCGGAGAT 
GTGGAGAAGC TGGACGAAGA GCTGAAGGCC TACTTGAAGA TGAACACCTA CCTGAAGTGG 
GGCGACCCGT GGTTCTGGGA TAAGCTGCGA TTTGCCCTGC CACATCGCAG ACCCGTCGGC 
AATATCGGTA ATGGAGCGCT AATCAAGACG GCGCTGAAAG GTTCCACGGA CGACAAGTTG 
GAGTTGATCA AACCGTCGCC GGTGACGCCA CCGCTAACCA CGCCGCCGGC GGAGGCAACC 
AAAAATCCGC TGGTAGCGCA GCTAAATGGC GTCACCCCGC ATCAGGCCAT AATGATTGCC 
AACGGCAAGA ATGGTCTGAC GAATCTGTAC ACACCCAATG GCAAGTCGCA CGGTAATGGC 
CACATCAACG GCGCCTTCAT CATTAACACG AATGCCAAAC AGAGCGACGT ATAGGACTGG 
GAGAAGGCGG AGCTGTTTCA GTTCGATCCC GACGATGAGC TATTGTTCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262995.1
CDS	580..3933
Translation	MSRLKAASELALLVIILQLLQWPGSEASFGRDACSEMSIDGLCQCAPIMSEYEIICPANAENPTFRLTIQPKDYVQIMCNLTDTTDYQQLPKKLRIGEVDRVQMRRCMLPGHTPIASILDYLGIVSPTTLIFESDNLGMNITRQHLDRLHGLKRFRFTTRRLTHIPANLLTDMRNLSHLELRANIEEMPSHLFDDLENLESIEFGSNKLRQMPRGIFGKMPKLKQLNLWSNQLHNLTKHDFEGATSVLGIDIHDNGIEQLPHDVFAHLTNVTDINLSANLFRSLPQGLFDHNKHLNEVRLMNNRVPLATLPSRLFANQPELQILRLRAELQSLPGDLFEHSTQITNISLGDNLLKTLPATLLEHQVNLLSLDLSNNRLTHLPDSLFAHTTNLTDLRLEDNLLTGISGDIFSNLGNLVTLVMSRNRLRTIDSRAFVSTNGLRHLHLDHNDIDLQQPLLDIMLQTQINSPFGYMHGLLTLNLRNNSIIFVYNDWKNTMLQLRELDLSYNNISSLGYEDLAFLSQNRLHVNMTHNKIRRIALPEDVHLGEGYNNNLVHVDLNDNPLVCDCTILWFIQLVRGVHKPQYSRQFKLRTDRLVCSQPNVLEGTPVRQIEPQTLICPLDFSDDPRERKCPRGCNCHVRTYDKALVINCHSGNLTHVPRLPNLHKNMQLMELHLENNTLLRLPSANTPGYESVTSLHLAGNNLTSIDVDQLPTNLTHLDISWNHLQMLNATVLGFLNRTMKWRSVKLSGNPWMCDCTAKPLLLFTQDNFERIGDRNEMMCVNAEMPTRMVELSTNDICPAEKGVFIALAVVIALTGLLAGFTAALYYKFQTEIKIWLYAHNLLLWFVTEEDLDKDKKFDAFISYSHKDQSFIEDYLVPQLEHGPQKFQLCVHERDWLVGGHIPENIMRSVADSRRTIIVLSQNFIKSEWARLEFRAAHRSALNEGRSRIIVIIYSDIGDVEKLDEELKAYLKMNTYLKWGDPWFWDKLRFALPHRRPVGNIGNGALIKTALKGSTDDKLELIKPSPVTPPLTTPPAEATKNPLVAQLNGVTPHQAIMIANGKNGLTNLYTPNGKSHGNGHINGAFIINTNAKQSDV*DWEKAELFQFDPDDELLFR

Target ORF information:

RefSeq Version	NM_001276066.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster toll, transcript variant C (Tl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276066.2

ORF Insert Sequence:

ATGAGTCGAC TAAAGGCCGC TTCCGAGCTG GCATTGCTTG TGATCATCCT GCAACTGCTA 
CAGTGGCCAG GATCTGAAGC ATCCTTCGGT CGGGATGCGT GCAGTGAGAT GAGCATAGAC 
GGACTGTGTC AGTGTGCACC CATAATGTCG GAATACGAAA TCATCTGCCC GGCGAATGCG 
GAGAATCCCA CGTTTAGGCT AACCATCCAG CCCAAAGACT ACGTGCAGAT CATGTGCAAT 
CTGACGGATA CCACGGACTA CCAGCAGCTG CCGAAAAAGT TGCGGATCGG TGAGGTCGAC 
AGGGTTCAGA TGCGACGGTG TATGCTGCCC GGTCACACGC CGATAGCCAG CATCCTGGAC 
TATTTGGGCA TAGTGTCGCC CACGACGCTG ATCTTTGAGA GCGACAACTT GGGCATGAAC 
ATAACACGGC AGCACTTGGA CCGTTTGCAC GGTCTAAAGC GATTCCGGTT TACCACTCGC 
CGTCTCACAC ACATTCCCGC CAACCTACTG ACGGATATGC GGAATCTGAG TCATCTCGAA 
CTAAGGGCAA ATATCGAGGA AATGCCATCG CATTTGTTCG ACGATCTGGA GAACCTGGAG 
TCAATTGAAT TCGGTAGCAA TAAGCTGAGG CAAATGCCGC GTGGCATTTT CGGCAAGATG 
CCCAAGCTGA AGCAGCTCAA TTTGTGGAGC AATCAACTGC ACAACCTCAC CAAACACGAC 
TTCGAAGGTG CCACCTCGGT GCTGGGCATA GATATTCACG ATAATGGCAT CGAACAGTTG 
CCGCACGACG TCTTTGCCCA TCTCACGAAT GTCACGGATA TCAATCTGAG CGCCAATCTC 
TTCCGTTCCC TGCCGCAGGG CCTATTCGAC CACAACAAAC ACCTGAACGA GGTGCGTCTG 
ATGAACAATC GAGTGCCCCT GGCCACGCTG CCTTCGCGTC TGTTTGCTAA TCAGCCGGAA 
CTGCAGATCT TACGGCTGAG AGCTGAACTG CAATCGTTGC CGGGTGACTT ATTCGAGCAT 
TCCACACAGA TCACAAACAT CTCGTTGGGA GACAATCTGC TGAAAACTCT GCCCGCCACC 
TTGCTGGAAC ATCAGGTGAA CCTGCTGAGC TTGGACTTGA GCAACAACCG GCTGACACAT 
CTGCCGGATT CACTCTTTGC ACATACCACC AATCTGACGG ATCTGCGTCT GGAGGATAAT 
TTGCTAACCG GCATTAGCGG CGATATATTC AGCAACTTGG GCAACCTTGT GACCCTCGTC 
ATGAGCAGGA ATCGACTGCG TACCATCGAT TCCAGAGCCT TTGTAAGCAC AAATGGACTG 
CGACATTTGC ATTTGGATCA CAACGACATT GATCTGCAAC AGCCATTGCT GGACATCATG 
CTCCAGACCC AGATCAACTC CCCGTTTGGT TACATGCACG GCCTTTTGAC CCTTAATCTA 
CGCAACAACT CAATCATATT CGTCTACAAC GACTGGAAGA ACACGATGCT CCAGTTGAGG 
GAGCTGGACT TGAGTTACAA CAACATTAGC TCGCTGGGCT ACGAGGATCT GGCGTTCCTG 
TCTCAGAATA GATTGCACGT AAACATGACG CACAATAAGA TACGGAGGAT AGCGCTGCCC 
GAGGATGTGC ACTTGGGCGA GGGCTACAAC AATAATCTGG TGCATGTGGA TCTCAACGAT 
AATCCCCTGG TCTGCGACTG TACGATCCTC TGGTTTATTC AGCTGGTGAG GGGCGTCCAT 
AAGCCGCAGT ATTCCAGGCA GTTCAAATTA CGCACAGACC GATTGGTTTG CAGCCAGCCA 
AATGTCTTGG AGGGTACTCC AGTTCGTCAG ATAGAACCAC AGACGCTCAT CTGTCCGCTG 
GACTTCTCCG ATGATCCACG GGAAAGGAAG TGTCCGCGCG GCTGCAACTG CCATGTGCGA 
ACCTACGACA AAGCACTGGT AATCAACTGC CATAGTGGCA ATCTGACACA TGTTCCTCGC 
TTGCCCAATC TGCATAAGAA TATGCAATTA ATGGAACTTC ACTTGGAGAA CAACACACTG 
CTCAGGCTTC CTTCGGCCAA TACACCCGGA TATGAGAGTG TAACTAGTCT GCATCTGGCT 
GGCAACAATC TAACCAGCAT CGATGTGGAT CAACTGCCTA CCAATCTCAC CCATTTGGAT 
ATAAGTTGGA ACCACCTGCA GATGCTGAAC GCAACCGTGC TGGGTTTCCT TAATCGTACC 
ATGAAGTGGC GCTCGGTGAA GCTGTCCGGG AATCCGTGGA TGTGTGATTG TACTGCCAAG 
CCGCTACTCC TCTTCACCCA GGACAATTTC GAAAGGATTG GAGATCGCAA TGAAATGATG 
TGCGTGAACG CCGAGATGCC AACACGAATG GTGGAACTGT CGACCAACGA CATTTGTCCG 
GCGGAAAAGG GCGTGTTCAT AGCCCTTGCT GTGGTGATTG CCTTGACTGG TCTTTTGGCC 
GGTTTCACAG CGGCACTGTA CTACAAATTC CAGACGGAGA TCAAGATTTG GCTGTATGCT 
CATAATCTCC TCCTGTGGTT TGTCACGGAG GAGGATCTGG ACAAGGACAA GAAGTTCGAT 
GCCTTCATCT CGTACTCGCA CAAGGACCAG AGCTTCATTG AGGACTACTT GGTGCCGCAA 
CTGGAGCACG GTCCTCAGAA GTTCCAGCTC TGTGTGCACG AACGAGACTG GCTGGTAGGC 
GGACACATTC CAGAGAACAT TATGCGCTCT GTGGCGGATT CGAGACGAAC GATTATTGTG 
CTCAGCCAGA ACTTTATCAA ATCGGAGTGG GCACGCCTTG AATTCAGGGC GGCGCACAGA 
TCGGCACTGA ACGAAGGACG CTCGCGCATT ATTGTGATCA TATACTCGGA CATCGGAGAT 
GTGGAGAAGC TGGACGAAGA GCTGAAGGCC TACTTGAAGA TGAACACCTA CCTGAAGTGG 
GGCGACCCGT GGTTCTGGGA TAAGCTGCGA TTTGCCCTGC CACATCGCAG ACCCGTCGGC 
AATATCGGTA ATGGAGCGCT AATCAAGACG GCGCTGAAAG GTTCCACGGA CGACAAGTTG 
GAGTTGATCA AACCGTCGCC GGTGACGCCA CCGCTAACCA CGCCGCCGGC GGAGGCAACC 
AAAAATCCGC TGGTAGCGCA GCTAAATGGC GTCACCCCGC ATCAGGCCAT AATGATTGCC 
AACGGCAAGA ATGGTCTGAC GAATCTGTAC ACACCCAATG GCAAGTCGCA CGGTAATGGC 
CACATCAACG GCGCCTTCAT CATTAACACG AATGCCAAAC AGAGCGACGT ATAGGACTGG 
GAGAAGGCGG AGCTGTTTCA GTTCGATCCC GACGATGAGC TATTGTTCCG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Tl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

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