CG5447 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG5447
Entrez Gene ID	43192
Full Name	CG5447 gene product from transcript CG5447-RA
Synonyms	Dmel\CG5447
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 97B3-97B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001276057.1	NP_001262986.1	uncharacterized protein, isoform B
NM_143221.4	NP_651478.1	uncharacterized protein, isoform A

Pathways from BioSystems

Homologies

Bos taurus (cattle)	GOLGA7B	NP_001070422.1
Gallus gallus (chicken)	LOC423837	XP_421706.4
Pan troglodytes (chimpanzee)	GOLGA7B	XP_003312768.1
Canis lupus familiaris (dog)	GOLGA7B	XP_003433658.1
Mus musculus (house mouse)	Golga7b	NP_081970.1
Homo sapiens (human)	GOLGA7B	NP_001010917.1
Rattus norvegicus (Norway rat)	Golga7b	XP_219889.5
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004120	XP_312998.4
	GOLGA7B	XP_002805821.1
Drosophila melanogaster (fruit fly)	CG5447	NP_001262986.1
Danio rerio (zebrafish)	golga7bb	XP_693951.3
Xenopus tropicalis (tropical clawed frog)	golga7b	XP_002936481.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG5447 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG5447 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06208	NM_143221.4 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG5447), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa08857	NM_001276057.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG5447), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG5447 ( NM_143221.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651478.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG5447), mRNA.

Price & Availability↑

CloneID	OFa06208
Clone ID Related Accession (Same CDS sequence)	NM_143221.4
Accession Version	NM_143221.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_143221.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCCAAG GCGGTGGCAC TACTCCAGCG GGCGGTAATC CCACAGCGCT CCAGGCGACC 
GGAGGGGGCG TGGTCTTCAG CAAGGTCTTC ATCCAGCGCG ATTACAGCGA GGGCACCTCC 
GTCAAGTTCC ATACACGGCT GCCCGCTGAG CTGGAGGGCA TGATTGAAAG ACACGTCTTC 
GAGGCCACCA TCAATAGGCT AAACGAGTTC TACGCCGAGG CGGAGGAGGG ATCCTGCGGC 
ACCTATTGCG AGGGCTGCAT TGGCTGCATC ACGGCCTACT TGATCTATAT GTGCTCCGAA 
ACGCACTATG AAAAGACCCT TCGCAAGATA TCCAAATTTG TGGCTTCCCA AAATGAACGC 
ATTTACAACA CCAAGGGACT GCAGCTGATC GATCCCACTT ATCGGGGACT TCGCGTCATA 
GAGATTACAA TATTCGATCG TCCGGGGCGA ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651478.1
CDS	132..587
Translation	MSQGGGTTPAGGNPTALQATGGGVVFSKVFIQRDYSEGTSVKFHTRLPAELEGMIERHVFEATINRLNEFYAEAEEGSCGTYCEGCIGCITAYLIYMCSETHYEKTLRKISKFVASQNERIYNTKGLQLIDPTYRGLRVIEITIFDRPGRT

Target ORF information:

RefSeq Version	NM_143221.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG5447), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143221.4

ORF Insert Sequence:

ATGTCCCAAG GCGGTGGCAC TACTCCAGCG GGCGGTAATC CCACAGCGCT CCAGGCGACC 
GGAGGGGGCG TGGTCTTCAG CAAGGTCTTC ATCCAGCGCG ATTACAGCGA GGGCACCTCC 
GTCAAGTTCC ATACACGGCT GCCCGCTGAG CTGGAGGGCA TGATTGAAAG ACACGTCTTC 
GAGGCCACCA TCAATAGGCT AAACGAGTTC TACGCCGAGG CGGAGGAGGG ATCCTGCGGC 
ACCTATTGCG AGGGCTGCAT TGGCTGCATC ACGGCCTACT TGATCTATAT GTGCTCCGAA 
ACGCACTATG AAAAGACCCT TCGCAAGATA TCCAAATTTG TGGCTTCCCA AAATGAACGC 
ATTTACAACA CCAAGGGACT GCAGCTGATC GATCCCACTT ATCGGGGACT TCGCGTCATA 
GAGATTACAA TATTCGATCG TCCGGGGCGA ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG5447 ( NM_001276057.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262986.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG5447), mRNA.

Price & Availability↑

CloneID	OFa08857
Clone ID Related Accession (Same CDS sequence)	NM_001276057.1
Accession Version	NM_001276057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCCAAG GCGGTGGCAC TACTCCAGCG GGCGGTAATC CCACAGCGCT CCAGGCGACC 
GGAGGGGGCG TGGTCTTCAG CAAGGTCTTC ATCCAGCGCG ATTACAGCGA GGGCACCTCC 
GTCAAGTTCC ATACACGGCT GCCCGCTGAG CTGGAGGGCA TGATTGAAAG ACACGTCTTC 
GAGGCCACCA TCAATAGGCT AAACGAGTTC TACGCCGAGG CGGAGGAGGG ATCCTGCGGC 
ACCTATTGCG AGGGCTGCAT TGGCTGCATC ACGGCCTACT TGATCTATAT GTGCTCCGAA 
ACGCACTATG AAAAGACCCT TCGCAAGATA TCCAAATTTG TGGCTTCCCA AAATGAACGC 
ATTTACAACA CCAAGGGACT GCAGCTGATC GATCCCACTT ATCGGGGACT TCGCGTCATA 
GAGATTACAA TATTCGATCG TCCGGGGCGA ACGTGACTTC CGCTTTCCCA TTCAGCCAAC 
GAGTTTTTCA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262986.1
CDS	132..626
Translation	MSQGGGTTPAGGNPTALQATGGGVVFSKVFIQRDYSEGTSVKFHTRLPAELEGMIERHVFEATINRLNEFYAEAEEGSCGTYCEGCIGCITAYLIYMCSETHYEKTLRKISKFVASQNERIYNTKGLQLIDPTYRGLRVIEITIFDRPGRT*LPLSHSANEFFM

Target ORF information:

RefSeq Version	NM_001276057.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG5447), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276057.1

ORF Insert Sequence:

ATGTCCCAAG GCGGTGGCAC TACTCCAGCG GGCGGTAATC CCACAGCGCT CCAGGCGACC 
GGAGGGGGCG TGGTCTTCAG CAAGGTCTTC ATCCAGCGCG ATTACAGCGA GGGCACCTCC 
GTCAAGTTCC ATACACGGCT GCCCGCTGAG CTGGAGGGCA TGATTGAAAG ACACGTCTTC 
GAGGCCACCA TCAATAGGCT AAACGAGTTC TACGCCGAGG CGGAGGAGGG ATCCTGCGGC 
ACCTATTGCG AGGGCTGCAT TGGCTGCATC ACGGCCTACT TGATCTATAT GTGCTCCGAA 
ACGCACTATG AAAAGACCCT TCGCAAGATA TCCAAATTTG TGGCTTCCCA AAATGAACGC 
ATTTACAACA CCAAGGGACT GCAGCTGATC GATCCCACTT ATCGGGGACT TCGCGTCATA 
GAGATTACAA TATTCGATCG TCCGGGGCGA ACGTGACTTC CGCTTTCCCA TTCAGCCAAC 
GAGTTTTTCA TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG5447 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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