Nf1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following Nf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa06160 NM_001014668.2
Latest version!
Drosophila melanogaster neurofibromin 1, transcript variant D (Nf1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OFa06161 NM_170252.3
Latest version!
Drosophila melanogaster neurofibromin 1, transcript variant C (Nf1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OFa06162 NM_170253.2
Latest version!
Drosophila melanogaster neurofibromin 1, transcript variant B (Nf1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OFa08849 NM_001276049.1
Latest version!
Drosophila melanogaster neurofibromin 1, transcript variant E (Nf1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OFa08850 NM_001276050.1
Latest version!
Drosophila melanogaster neurofibromin 1, transcript variant F (Nf1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa06160
    Clone ID Related Accession (Same CDS sequence) NM_001014668.2
    Accession Version NM_001014668.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8295bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product neurofibromin 1, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_001014668.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGACCCAGA AGCCAGGCGA GTGGGCTAGC GCCCTTTTGG CACGTTTCGA GGATCAGCTG 
    CCAAACAGAA TCGGTGCCTA TGGCACGCAG GCCAGGATGA GCCAGGACCA ATTGGTGGCC
    TGTCTGATCC ACATATCGCG CTACCGCTTC TCCCTTGTCA TATCCGGTCT CACCAAGATG
    CTGCAGAGGG TCAATGAGGC GGCCCTTCAA AATCGCCATG AGCCAGAGCG TTGCTACTTT
    GAGTCGCTGG TCATCATACT GACCACTCTG GAACGCTGTC TGACCAACCA GACCAAGGAC
    ACAGCACGTT TTGAAGAGGC CATGAATGTG AAGCTTCTGC TGCGCGAGAT CTCCCAGTTT
    GTCGACGTCC AGAGCGACAG CAATCCGAAT GCCGCCCAGC TCAAGGCCCT GGCATCCAAG
    GTGCTCTTTG CCTTGTCCCA GAATCACTTC TCGGCGGTGT TCAATCGCAT ATCGGCCAGG
    ATTCAAGAGC TGACATCCTG TTCCGAGGAA AACCCGGACT ACAACGACAT AGAATTAATT
    CAACACATCG ATATGGATAT GATAAAGCTA ACCAAGCTGC TACAAGAAAC CATCACAAAG
    TTTCGATCGA AGCGTGCTCC ACCTTTGATC TTGCTTTACT CGCTGGAGAA GGCTATTTGG
    AACTGGATCG AGTACCATCC ACAAGAGTTC CAGGACTTGC AACGAGGAAC CAATCGAGAT
    ATATCAACCT GCTGGGAACC CCTGATGGAC TTTGTAGAGT ACTTTAAAAC CGAAAACAAG
    AAAAGCAAGA CTCTTGTTTG GCCGCTTCAA ATGCTCTTGC TGATATTGAA TCCCTCTTGC
    CTTGAGGCTG TTGTTAACGA ACTTCAGCAG TCGGAGAAAG AGAAGGAAAA GGACAAGGAA
    AAGGTTGCCT CGAAGTCAGC GCAATCCACG TCCCGGGACA AGGACTTCTC GGCCAAGCAG
    TTCATCGAGA GTATCAAGAG AGGCTTGGGC CAGCACTCAC CATCCAAACA GGTGACCGAA
    TCTGCGGCAA TTGCCTGTGT AAAGCTGTGC AAAGCATCCA CCTACATCAA CAACACCGAC
    TCCAATAATG TGGTCTTCAA GTTGGTGCAA TTCTTTATCA ACGATCTCAA GGCACTGCTC
    TTTAATCCAG CAAAGCCCTT TTCGCGCGGT CAGGGGTACA ACTTCGCTGA TATCGAGCTG
    ATGATTGACT GCTGGGTGTC TTGTTTCCGG ATTAATCCTC ACAACATCGA GGCTCTAAAG
    GTTTGCCTGA ATTTGTCCTC GCCGCAGGCT TACCATTTTG TAATTGTGTG CTCTCTGCTA
    AGGTTGGCTC ACATATACGT TGACTTCCGT TTGCAGAACA AGAACCCCTT TCGAATAGTA
    AACCAACCGA GATTGTCTTG GTGGCCGCAA ACGGATGTGG TTCACTACCG TTCTGCTGAG
    CTACGAGCTT TGTTTACGGA TACGCTCAAC AAGGCCACCC AGGGTTATAT AGCCCATACG
    CCACTGCGCT ACATAACCTC CCTGACGCTC AAATCTAAAG ATACCCAGAA AGGTTTAACC
    CGCGCTGAAG AGGGTCCCGC CCACAAAATG CTATTACTGC TACTCGTACG GCTAATTCAT
    GCAGATCCCA CGCTTTTGTT GAATACCCAA GGAAAAGTGG CACATGAAGT GCAGAGCTCC
    ACATTGGAGC TCATCAACGG CTTAGTTAGT TTGGTGCATC AAACTACCAT GCCCGATGTG
    GCACAAGAGG CTATGGAGGC GCTGCTTGCT CTGCACGCCC CGGAGAAAAT AGAGGTTTGG
    AATCCAGAGG CTCCGATCAA TACGTTCTGG GACGTTAGCT CCCAAGTACT GTTTTCAATC
    TCGCAAAAGC TCATTCAACA CCAAATTGCC AATTACACTG ATGTCTTAAA GTGGCTACGC
    GAGATCCTCA TCTGTCGGAA TACTTTTCTT CAGCGACACA AGGATTATGC ACATGTGGGA
    AGTCAGATTG CCATTTGCAA GCAGGCTCAC ATTAAGATGG AAGTTGTTTT CTTCATGTAC
    CTGTGGAGTG TTGATTTAGA TGCGGTGCTG ACATCGCTAT CGTGTTTTGG ACTACTATGC
    GAGGAGGCTG AGATTTGCTG CAGTTCTGAT GAATTGACAG TCGGATTTAT TATGCCGAAT
    TACCACATTT ACCAGGAGCT GGCTCAGTTG TCCACATCTG CAACGGACTC TCGCATTTGC
    TTTTTTGACA ACACGCACGG CAATGTGCTG AGCCGTCTTA CACTTCAAAA GCGCATAATG
    ACCCTATTGC GCAAGATCGA GCACTGTGTT CATGGTGTTC AGCCCGCCTG GGAGGAAACC
    TTCCGCAACT GGGAAGTATC CAGCAAGGTT TTGCAAACGT ACCCCAAATG TAAAGGAGAA
    GATGGCCAAG CGGAGGTTTT CCATCGTGGC ATGGGCAAGC GGCGAGCGAG TCACCAAAGC
    TCAGAGCACG ATTTGGAGGA ACAAATTAAT GAATGGGCCA ATATGACATG GTTTCTATTA
    GCCTTGGGTG GCGTCTGCCT TCATAAACGT AGCAGCAGTC GTCAAATGCT GCTGCAGCAA
    TCACAGAACA ATGCTTCTTT GGGATCACTT GCTCAAAACT CTCTTTACTC AAGCTCCACA
    AGTTCTGGAC ATGGCTCTCT GCATCCTAGC ACGGTTTCAC TATCCACTCT TCCCCCAGCA
    CCGCCACAAG ATGTCAGCTA TTGTCCTGTA ACACAATTCG TGGGCCAACT ATTGCGCCTG
    TTAGTTTGCA GCAACGAAAA AATTGGACTT AATATTCAGA AAAATGTGAA GGAACTGGTG
    GGCGAGGAGA TGTCCACCCA ACTGTATCCT ATACTCTTCG ACCAGGTTAG AGCCATCGTG
    GAGAAGTTCT TCGATCAGCA AGGTCAGGTG AACGTCAATG TGACCGACAT CAACACGCAG
    TTTATCGAGC ACACCATCTA CATAATGAAA TCAATTCTGG ATCCCAAAGC CAACAAGGAT
    CCCAACAACG ATCAACCCTC GCCATCGGAA CATTTAGGTG TTACAAGCAT CGAAGGCATG
    ATGCTAGGAA TAGTGCGCTA CGTTCGCCAC CTGGATATGA CTGTTTATGC CATTCGAATC
    AAGACAAAAT TGTGCCAGCT TGTGGAAGTA ATGATGAAGC GACGCGACGA CCTCGCCTTT
    CGCCAGGAGA TGAAGTTCCG GAATAAACTG GTTGAATACT TGACCGACTG GGTGATGGGC
    ACCTCCCATC AGATTGCACC GCCCAGCTCG GCGGATGCCG CTATTCTTAC CAACACATCC
    CTCATATTTC GCGATCTAGA CCAAGCCTGC ATGGAGGCAG TGGCTGCCTT GCTTCGGGGC
    CTTCCCCTTC AGCCTGAGGA ATCGGATCGA GGCGATTTGA TGGATGCAAA GAGTGCGCTC
    TTTTTAAAGT ACTTTACCCT ATTTATGAAC CTGCTGAATG ATTGCATCGA TAGCTCCGAG
    GCGGAGAAGG AAATGAATAA TACCCCACTA TTGCCTCCGC GCCCTCGAAT GGCAGCTGGA
    AAACTGACCG CTCTGCGAAA TGCCACTATT CTAGCCATGT CTAATTTGTT GGGTGCCAAC
    ATTGACTCTG GTTTGATGCA CTCCATCGAT CTGGGTTATA ATCCTGATTT GCAAACGCGT
    GCCGCTTTCA TGGAGGTGTT GACTCAAATC CTGCAACAAG GCACCGAATT CGATACCTTG
    GCCGAAACTG TTTTAGCTGA TCGATTTGAG CAACTGGTCC AACTTGTTAC AATGATCAGC
    GACAAAGGAG AACTTCCCAT AGCAATGGCT TTGGCTAATG TGGTGACTAC ATCCCAAATG
    GACGAGCTGG CTAGGGTTCT GGTCACCCTA TTCGACGCCA AACACCTGTT ATCGCCACTC
    CTATGGAATA TGTTTTATCG CGAGGTGGAG GTCTCAGACT GCATGCAGAC ACTTTTCCGT
    GGAAATTCTT TGGGCAGCAA AATCATGGCC TTTTGCTTTA AAATATACGG CGCGAGCTAT
    CTCCAAATGC TACTAGAGCC TTTAATTCGT CCGCTTCTGG ATGAGGAGGA GGAGACCTGT
    TTTGAGGTGG ATCCAGCTCG TCTGGATCCA ACAGAAGATA TTGAACAGCA CAGGAACAAC
    TTGATTGCCC TAACGCAGAA GGTGTTTGAC GCTATTATCA ACTCGTCGGA TCGCTTTCCC
    CCGCAGCTGC GATCCATGTG CCATTGCCTG TACCAAGTGC TAAGCAAACG CTTCCCGAAT
    CTTCTTCAAA ACAATATCGG TGCTGTAGGC ACAGTCATCT TCTTGCGGTT CATCAATCCC
    GCCATAGTTT CCCCACAGGA ATTGGGAATC GTTGACAAAC AAGTGCACAG CTCGGCCAAA
    CGAGGTCTAA TGCTAATGTC CAAGATCCTT CAAAACATTG CTAACCACGT GGAGTTCTCC
    AAGGAGCAGC ACATGTTGTG TTTCAACGAT TTTCTGCGCG ATCACTTTGA AGCTGGGCGG
    CGATTCTTCA TACAGATTGC ATCAGACTGT GAGACCGTGG ATCAGACCTC GCACAGCATG
    AGTTTTATTT CAGATGCGAA CGTACTGGCA CTGCATCGGT TGCTGTGGAC GCATCAGGAG
    AAGATCGGCG ACTATCTGTC TAGCAGTCGA GACCACAAGG CGGTTGGAAG GCGGCCCTTC
    GACAAGATGG CTACTTTGCT AGCATACCTG GGACCACCGG AGCACAAGCC CGTGGATTCG
    CACATGATGT TCAGCTCGTA TGCACGCTGG AGCTCCATTG ACATGTCGTC GACAAACTTC
    GAGGAGATCA TGGTCAAGCA CCAAATGCAC GAGAAGGAGG AATTCAAGAC CCTCAAGTCG
    ATGAACATAT TCTACCAGGC CGGAACAAGC AAATCTGGCT ATCCTGTCTT TTACTATATA
    GCAAGACGAT ACAAGATTGG AGAAACGAAT GGAGACTTAC TTATATATCA CGTCATACTC
    ACTCTGAAAC CATTCTGCCA CTCGCCCTTC GAGGTGGTCA TCGATTTTAC GCACACCTGT
    TCAGATAATC GGTTCCGCAC CGAGTTTCTG CAGAAGTGGT TTTATGTCCT GCCCACGGTA
    GCCTACGAAA ATGTCCATGC TGTGTACATC TATAACTGTA ACTCGTGGGT GCGCGAATAT
    ACCAAGTTCC ACGATCGCAT TCTGGCACCA TTAAAGGGAA ATCGCAAACT TCTGTTCCTG
    GAGTCACCCA ACAAACTTAC TGATTTCATC GACGCGGAGC AACAGAAATT GCCTGGAGCA
    ACTCTTTCCT TGGACGAAGA TTTAAAGGTA TTCAGCAACG CGCTGAAGCT CAGCCATAAA
    GACACAAAGG TGGCTATCAA AGTGGGTCCC ACCGCATTGC AAATTACATC TGCCGAAAAG
    ACAAAGGTCT TGGCTCACTC CGTGCTCCTG AACGATGTAT ACTACGCCTC TGAAATTGAG
    GAGGTGTGCT TGGTGGACGA TAACCAGTTC ACCCTGTCTA TAACTAACGA AAGTGGCCAG
    CTTAGCTTTA TTCACAACGA TTGCGACAAC ATTGTTCAAG CCATCATACA CATCCGAAAT
    CGATGGGAGC TTAGTCAGCC GGACTCCGTG ACGGTTCACC AAAAGATCCG ACCAAAAGAT
    GTGCCTGGAA CGCTGCTGAA CATGGCGCTA CTCAATCTGG GATCATGTGA CCCCAATCTA
    AGGACTGCTG CCTACAATTT GCTGTGTGCT TTGACCGCTA CTTTTGATCT AAAGATTGAG
    GGTCAACTGT TGGAGACCCA AGGACTTTGC ATACCCTCAA ATAATACCAT ATTTATCAAA
    TCCGTTAGCG AAAAGCTGGC CACCAATGAA CCGCATCTGA CTCTGGAATT CCTTGAGGAG
    TCCATTCAGG GCTTCCAGCG CAGCACCATA GAGCTGAAAC ATTTGTGTTT GGAGTACATG
    ACGCCATGGT TAAAGAACCT AGTCAAATTT TGCAAGTCCA ACGATGACTC CAAAAAGCTT
    AAGGTCTCCC AAATTCTGGA CAAGCTCATC AATTTAACTA TTGACCAAAA GGAAATGTAT
    CCCTCAGTGC AGGCCAAGAT CTGGGGATCA ATTGGACAGA TCCCCGAGCT GATCGACATG
    GTTTTGGATA ACTTTTTGCA CAAATCGATC ACATATGGCT TGGGATCACC ACAGGTGGAG
    ATTATGGCTG ACACGGCAGT GGCTCTCGCT TCAGCGAATG TTCAGCTGGT GTCCAAAAAG
    GTCATAACGC GAATATGCCG AGTCATGGAC AAATCCTGCA CAAATCCCAC ACAATATCTG
    GAGCAGCATA TGATGTGGGA CGATATTGCA ATTCTTGGTC GATACCTGCT CATGTTGTCG
    TTCAACAATT GTTTGGATGT GGCCACATCG GTGCCATATC TATTCCACAC CATTACGTTT
    TTGGTATGTT CAGGATCCCT GTCGATGCGA GCCTCTACTC ATGGCCTAGT GATCAACATC
    ATCCACTCGC TGTGCACATG CACAAATCCC TCATTTTCAG AGGAGGCGCA GCGAGTACTC
    CGACTGTCCC TGGATGAATT CTCACTGCCC AAGTTCTATC TACTCTTCGG CATCAGCAAG
    GTCAAGTCCG CAGCAGTTAC CGCTTTCCGC TCCAGCTGCC GTCACCCTAC AGATAAATGG
    CTAGGAAATG AAAGGGTTAC CCAGCCTCTG CCCGCCGATC GAGAGCGTTT ATCCCTACCA
    TCGCTTGAGG TTATCACGGA TGCCCTGCTG GAAATCATGG AGGCTTGCAT GCGAGATGTT
    CCGGACTGCG AATGGCTCAA CACCTGGACC TCTTTGGCTC GCAGTTTTGC TTTCTGTTAC
    AATCCAGCGC TACAGCCTAG AGCCCTTATA GTTTACGGGT GCATCAGCAA GAGTGTTACC
    GATCACGAGG TCAAGCAGTT ACTACGCATC CTGGTTAAGG CCCTCGAATC TTTCAACGAT
    CTCATTCTGA TCGAGGCTCT AGTAATGTGC TTAACTCGCA TTCAACCACT ACTCCGACCA
    GAGTCGCCCA TTCATCGGGC CCTCTTCTGG GTGGCCATTT CGGTGCTGCA GTTGGACGAG
    ATTACCCTGT ACGGCGCTGG ACTAGCTCTG CTTGAACAAA ATCTACACAC GCTTAAGTCA
    CAGGGCTGTT TTGACAAGAA GGAGACCATA GCCGAGGTCA TGATGAAGAC AAGGGAAAAG
    CTGGAGTGGC ATTTCAAGCA ACTGGATCAC GCCGTCGGCC TCTCCTTCCG CAGCAATTTC
    CACTTTGCCC TAGTGGGACA CTTGATTAAG GGCTTCCGCC ATCCTACACC TACAACCGTC
    TCACGCACCT CTCGTGTGCT GACGATGCTC TTGGGCATAA TAGCCAAACC CCTGCATCGG
    GACAAGTTCG AAGTAACACC TGACAGTGTG GCCTATTTGA CTGCGCTGGT GGCTGTCTCC
    GAGGAAGTCC GTTCCAGATG TCACGTAAAA CATGCCCTTC CCCGCTGGCC AGCCGATCTG
    AGTAGTAGTG TGGAAAATGG TGAAGCATCC GGCGGAGTGC AAGCTATCGG CCTGCCCCTG
    TCACGCCGTC AAAAAAGTTG GGACATCCTG GATCAGTCCG CCTTGCAGTT TGCCCGCCAA
    CACAAGGTTC CCACTCTTCA GAACGCGCGA GTTTTGTTCA AAACTCAACG CTCATTCTCG
    GTGCCGACGA CCAAAGATCC GAACAATGCC ACCGGCATCG AAGAACGTCA GGAACGCGGT
    TCGCGTTCGT CGGTGTCCAA CGAGTCCAAC GTACTGCTCG ATCCCGAGGT CTTACCTGAC
    CTGTCCATTC AAGCCCTGGT ACTGACTGTT TTGGCCACCT TGGTCAAGTA CTCTTCGGAT
    GAAGGCGAAA CGCGGGTTTT GTATCAGTAT TTGGCCGAAG GATCGGTGGT CTTTCCAAAG
    GTGTTTCCAG TTATCCATTC ACTGCTGGAC CAAAAGATAA ACAACATTTT GTCAGTGTCA
    CACGACCAAG TGGTGCTCAA CTCTGTGCAG AACATTATAC AAAATATGCT GGCCAGCGAA
    GATCCTTCCC AGCAGCAGCT GCACTTCCTG CAGAGTTGTG GATTTGGCGG ACTCTGGCGA
    TTTGCTGGTC CCTTCACCAA GTACAATATG ATGGGTGAGT CCTCGGAGCT GTTTGTCAAC
    TGTTTGGAGG CCATGGTTGA GACCTGTTTG CCAGGCGACG AATCCGCGCC GGTGCCACCA
    TCTCCGCGTC CATACAACCT GAGCTCCAGT CTGAGTAGCC TGACCCTGGG ATCACCCACG
    GATAAAGCTG CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001014668.1
    CDS280..8574
    Translation

    Target ORF information:

    RefSeq Version NM_001014668.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster neurofibromin 1, transcript variant D (Nf1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001014668.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGACCCAGA AGCCAGGCGA GTGGGCTAGC GCCCTTTTGG CACGTTTCGA GGATCAGCTG 
    CCAAACAGAA TCGGTGCCTA TGGCACGCAG GCCAGGATGA GCCAGGACCA ATTGGTGGCC
    TGTCTGATCC ACATATCGCG CTACCGCTTC TCCCTTGTCA TATCCGGTCT CACCAAGATG
    CTGCAGAGGG TCAATGAGGC GGCCCTTCAA AATCGCCATG AGCCAGAGCG TTGCTACTTT
    GAGTCGCTGG TCATCATACT GACCACTCTG GAACGCTGTC TGACCAACCA GACCAAGGAC
    ACAGCACGTT TTGAAGAGGC CATGAATGTG AAGCTTCTGC TGCGCGAGAT CTCCCAGTTT
    GTCGACGTCC AGAGCGACAG CAATCCGAAT GCCGCCCAGC TCAAGGCCCT GGCATCCAAG
    GTGCTCTTTG CCTTGTCCCA GAATCACTTC TCGGCGGTGT TCAATCGCAT ATCGGCCAGG
    ATTCAAGAGC TGACATCCTG TTCCGAGGAA AACCCGGACT ACAACGACAT AGAATTAATT
    CAACACATCG ATATGGATAT GATAAAGCTA ACCAAGCTGC TACAAGAAAC CATCACAAAG
    TTTCGATCGA AGCGTGCTCC ACCTTTGATC TTGCTTTACT CGCTGGAGAA GGCTATTTGG
    AACTGGATCG AGTACCATCC ACAAGAGTTC CAGGACTTGC AACGAGGAAC CAATCGAGAT
    ATATCAACCT GCTGGGAACC CCTGATGGAC TTTGTAGAGT ACTTTAAAAC CGAAAACAAG
    AAAAGCAAGA CTCTTGTTTG GCCGCTTCAA ATGCTCTTGC TGATATTGAA TCCCTCTTGC
    CTTGAGGCTG TTGTTAACGA ACTTCAGCAG TCGGAGAAAG AGAAGGAAAA GGACAAGGAA
    AAGGTTGCCT CGAAGTCAGC GCAATCCACG TCCCGGGACA AGGACTTCTC GGCCAAGCAG
    TTCATCGAGA GTATCAAGAG AGGCTTGGGC CAGCACTCAC CATCCAAACA GGTGACCGAA
    TCTGCGGCAA TTGCCTGTGT AAAGCTGTGC AAAGCATCCA CCTACATCAA CAACACCGAC
    TCCAATAATG TGGTCTTCAA GTTGGTGCAA TTCTTTATCA ACGATCTCAA GGCACTGCTC
    TTTAATCCAG CAAAGCCCTT TTCGCGCGGT CAGGGGTACA ACTTCGCTGA TATCGAGCTG
    ATGATTGACT GCTGGGTGTC TTGTTTCCGG ATTAATCCTC ACAACATCGA GGCTCTAAAG
    GTTTGCCTGA ATTTGTCCTC GCCGCAGGCT TACCATTTTG TAATTGTGTG CTCTCTGCTA
    AGGTTGGCTC ACATATACGT TGACTTCCGT TTGCAGAACA AGAACCCCTT TCGAATAGTA
    AACCAACCGA GATTGTCTTG GTGGCCGCAA ACGGATGTGG TTCACTACCG TTCTGCTGAG
    CTACGAGCTT TGTTTACGGA TACGCTCAAC AAGGCCACCC AGGGTTATAT AGCCCATACG
    CCACTGCGCT ACATAACCTC CCTGACGCTC AAATCTAAAG ATACCCAGAA AGGTTTAACC
    CGCGCTGAAG AGGGTCCCGC CCACAAAATG CTATTACTGC TACTCGTACG GCTAATTCAT
    GCAGATCCCA CGCTTTTGTT GAATACCCAA GGAAAAGTGG CACATGAAGT GCAGAGCTCC
    ACATTGGAGC TCATCAACGG CTTAGTTAGT TTGGTGCATC AAACTACCAT GCCCGATGTG
    GCACAAGAGG CTATGGAGGC GCTGCTTGCT CTGCACGCCC CGGAGAAAAT AGAGGTTTGG
    AATCCAGAGG CTCCGATCAA TACGTTCTGG GACGTTAGCT CCCAAGTACT GTTTTCAATC
    TCGCAAAAGC TCATTCAACA CCAAATTGCC AATTACACTG ATGTCTTAAA GTGGCTACGC
    GAGATCCTCA TCTGTCGGAA TACTTTTCTT CAGCGACACA AGGATTATGC ACATGTGGGA
    AGTCAGATTG CCATTTGCAA GCAGGCTCAC ATTAAGATGG AAGTTGTTTT CTTCATGTAC
    CTGTGGAGTG TTGATTTAGA TGCGGTGCTG ACATCGCTAT CGTGTTTTGG ACTACTATGC
    GAGGAGGCTG AGATTTGCTG CAGTTCTGAT GAATTGACAG TCGGATTTAT TATGCCGAAT
    TACCACATTT ACCAGGAGCT GGCTCAGTTG TCCACATCTG CAACGGACTC TCGCATTTGC
    TTTTTTGACA ACACGCACGG CAATGTGCTG AGCCGTCTTA CACTTCAAAA GCGCATAATG
    ACCCTATTGC GCAAGATCGA GCACTGTGTT CATGGTGTTC AGCCCGCCTG GGAGGAAACC
    TTCCGCAACT GGGAAGTATC CAGCAAGGTT TTGCAAACGT ACCCCAAATG TAAAGGAGAA
    GATGGCCAAG CGGAGGTTTT CCATCGTGGC ATGGGCAAGC GGCGAGCGAG TCACCAAAGC
    TCAGAGCACG ATTTGGAGGA ACAAATTAAT GAATGGGCCA ATATGACATG GTTTCTATTA
    GCCTTGGGTG GCGTCTGCCT TCATAAACGT AGCAGCAGTC GTCAAATGCT GCTGCAGCAA
    TCACAGAACA ATGCTTCTTT GGGATCACTT GCTCAAAACT CTCTTTACTC AAGCTCCACA
    AGTTCTGGAC ATGGCTCTCT GCATCCTAGC ACGGTTTCAC TATCCACTCT TCCCCCAGCA
    CCGCCACAAG ATGTCAGCTA TTGTCCTGTA ACACAATTCG TGGGCCAACT ATTGCGCCTG
    TTAGTTTGCA GCAACGAAAA AATTGGACTT AATATTCAGA AAAATGTGAA GGAACTGGTG
    GGCGAGGAGA TGTCCACCCA ACTGTATCCT ATACTCTTCG ACCAGGTTAG AGCCATCGTG
    GAGAAGTTCT TCGATCAGCA AGGTCAGGTG AACGTCAATG TGACCGACAT CAACACGCAG
    TTTATCGAGC ACACCATCTA CATAATGAAA TCAATTCTGG ATCCCAAAGC CAACAAGGAT
    CCCAACAACG ATCAACCCTC GCCATCGGAA CATTTAGGTG TTACAAGCAT CGAAGGCATG
    ATGCTAGGAA TAGTGCGCTA CGTTCGCCAC CTGGATATGA CTGTTTATGC CATTCGAATC
    AAGACAAAAT TGTGCCAGCT TGTGGAAGTA ATGATGAAGC GACGCGACGA CCTCGCCTTT
    CGCCAGGAGA TGAAGTTCCG GAATAAACTG GTTGAATACT TGACCGACTG GGTGATGGGC
    ACCTCCCATC AGATTGCACC GCCCAGCTCG GCGGATGCCG CTATTCTTAC CAACACATCC
    CTCATATTTC GCGATCTAGA CCAAGCCTGC ATGGAGGCAG TGGCTGCCTT GCTTCGGGGC
    CTTCCCCTTC AGCCTGAGGA ATCGGATCGA GGCGATTTGA TGGATGCAAA GAGTGCGCTC
    TTTTTAAAGT ACTTTACCCT ATTTATGAAC CTGCTGAATG ATTGCATCGA TAGCTCCGAG
    GCGGAGAAGG AAATGAATAA TACCCCACTA TTGCCTCCGC GCCCTCGAAT GGCAGCTGGA
    AAACTGACCG CTCTGCGAAA TGCCACTATT CTAGCCATGT CTAATTTGTT GGGTGCCAAC
    ATTGACTCTG GTTTGATGCA CTCCATCGAT CTGGGTTATA ATCCTGATTT GCAAACGCGT
    GCCGCTTTCA TGGAGGTGTT GACTCAAATC CTGCAACAAG GCACCGAATT CGATACCTTG
    GCCGAAACTG TTTTAGCTGA TCGATTTGAG CAACTGGTCC AACTTGTTAC AATGATCAGC
    GACAAAGGAG AACTTCCCAT AGCAATGGCT TTGGCTAATG TGGTGACTAC ATCCCAAATG
    GACGAGCTGG CTAGGGTTCT GGTCACCCTA TTCGACGCCA AACACCTGTT ATCGCCACTC
    CTATGGAATA TGTTTTATCG CGAGGTGGAG GTCTCAGACT GCATGCAGAC ACTTTTCCGT
    GGAAATTCTT TGGGCAGCAA AATCATGGCC TTTTGCTTTA AAATATACGG CGCGAGCTAT
    CTCCAAATGC TACTAGAGCC TTTAATTCGT CCGCTTCTGG ATGAGGAGGA GGAGACCTGT
    TTTGAGGTGG ATCCAGCTCG TCTGGATCCA ACAGAAGATA TTGAACAGCA CAGGAACAAC
    TTGATTGCCC TAACGCAGAA GGTGTTTGAC GCTATTATCA ACTCGTCGGA TCGCTTTCCC
    CCGCAGCTGC GATCCATGTG CCATTGCCTG TACCAAGTGC TAAGCAAACG CTTCCCGAAT
    CTTCTTCAAA ACAATATCGG TGCTGTAGGC ACAGTCATCT TCTTGCGGTT CATCAATCCC
    GCCATAGTTT CCCCACAGGA ATTGGGAATC GTTGACAAAC AAGTGCACAG CTCGGCCAAA
    CGAGGTCTAA TGCTAATGTC CAAGATCCTT CAAAACATTG CTAACCACGT GGAGTTCTCC
    AAGGAGCAGC ACATGTTGTG TTTCAACGAT TTTCTGCGCG ATCACTTTGA AGCTGGGCGG
    CGATTCTTCA TACAGATTGC ATCAGACTGT GAGACCGTGG ATCAGACCTC GCACAGCATG
    AGTTTTATTT CAGATGCGAA CGTACTGGCA CTGCATCGGT TGCTGTGGAC GCATCAGGAG
    AAGATCGGCG ACTATCTGTC TAGCAGTCGA GACCACAAGG CGGTTGGAAG GCGGCCCTTC
    GACAAGATGG CTACTTTGCT AGCATACCTG GGACCACCGG AGCACAAGCC CGTGGATTCG
    CACATGATGT TCAGCTCGTA TGCACGCTGG AGCTCCATTG ACATGTCGTC GACAAACTTC
    GAGGAGATCA TGGTCAAGCA CCAAATGCAC GAGAAGGAGG AATTCAAGAC CCTCAAGTCG
    ATGAACATAT TCTACCAGGC CGGAACAAGC AAATCTGGCT ATCCTGTCTT TTACTATATA
    GCAAGACGAT ACAAGATTGG AGAAACGAAT GGAGACTTAC TTATATATCA CGTCATACTC
    ACTCTGAAAC CATTCTGCCA CTCGCCCTTC GAGGTGGTCA TCGATTTTAC GCACACCTGT
    TCAGATAATC GGTTCCGCAC CGAGTTTCTG CAGAAGTGGT TTTATGTCCT GCCCACGGTA
    GCCTACGAAA ATGTCCATGC TGTGTACATC TATAACTGTA ACTCGTGGGT GCGCGAATAT
    ACCAAGTTCC ACGATCGCAT TCTGGCACCA TTAAAGGGAA ATCGCAAACT TCTGTTCCTG
    GAGTCACCCA ACAAACTTAC TGATTTCATC GACGCGGAGC AACAGAAATT GCCTGGAGCA
    ACTCTTTCCT TGGACGAAGA TTTAAAGGTA TTCAGCAACG CGCTGAAGCT CAGCCATAAA
    GACACAAAGG TGGCTATCAA AGTGGGTCCC ACCGCATTGC AAATTACATC TGCCGAAAAG
    ACAAAGGTCT TGGCTCACTC CGTGCTCCTG AACGATGTAT ACTACGCCTC TGAAATTGAG
    GAGGTGTGCT TGGTGGACGA TAACCAGTTC ACCCTGTCTA TAACTAACGA AAGTGGCCAG
    CTTAGCTTTA TTCACAACGA TTGCGACAAC ATTGTTCAAG CCATCATACA CATCCGAAAT
    CGATGGGAGC TTAGTCAGCC GGACTCCGTG ACGGTTCACC AAAAGATCCG ACCAAAAGAT
    GTGCCTGGAA CGCTGCTGAA CATGGCGCTA CTCAATCTGG GATCATGTGA CCCCAATCTA
    AGGACTGCTG CCTACAATTT GCTGTGTGCT TTGACCGCTA CTTTTGATCT AAAGATTGAG
    GGTCAACTGT TGGAGACCCA AGGACTTTGC ATACCCTCAA ATAATACCAT ATTTATCAAA
    TCCGTTAGCG AAAAGCTGGC CACCAATGAA CCGCATCTGA CTCTGGAATT CCTTGAGGAG
    TCCATTCAGG GCTTCCAGCG CAGCACCATA GAGCTGAAAC ATTTGTGTTT GGAGTACATG
    ACGCCATGGT TAAAGAACCT AGTCAAATTT TGCAAGTCCA ACGATGACTC CAAAAAGCTT
    AAGGTCTCCC AAATTCTGGA CAAGCTCATC AATTTAACTA TTGACCAAAA GGAAATGTAT
    CCCTCAGTGC AGGCCAAGAT CTGGGGATCA ATTGGACAGA TCCCCGAGCT GATCGACATG
    GTTTTGGATA ACTTTTTGCA CAAATCGATC ACATATGGCT TGGGATCACC ACAGGTGGAG
    ATTATGGCTG ACACGGCAGT GGCTCTCGCT TCAGCGAATG TTCAGCTGGT GTCCAAAAAG
    GTCATAACGC GAATATGCCG AGTCATGGAC AAATCCTGCA CAAATCCCAC ACAATATCTG
    GAGCAGCATA TGATGTGGGA CGATATTGCA ATTCTTGGTC GATACCTGCT CATGTTGTCG
    TTCAACAATT GTTTGGATGT GGCCACATCG GTGCCATATC TATTCCACAC CATTACGTTT
    TTGGTATGTT CAGGATCCCT GTCGATGCGA GCCTCTACTC ATGGCCTAGT GATCAACATC
    ATCCACTCGC TGTGCACATG CACAAATCCC TCATTTTCAG AGGAGGCGCA GCGAGTACTC
    CGACTGTCCC TGGATGAATT CTCACTGCCC AAGTTCTATC TACTCTTCGG CATCAGCAAG
    GTCAAGTCCG CAGCAGTTAC CGCTTTCCGC TCCAGCTGCC GTCACCCTAC AGATAAATGG
    CTAGGAAATG AAAGGGTTAC CCAGCCTCTG CCCGCCGATC GAGAGCGTTT ATCCCTACCA
    TCGCTTGAGG TTATCACGGA TGCCCTGCTG GAAATCATGG AGGCTTGCAT GCGAGATGTT
    CCGGACTGCG AATGGCTCAA CACCTGGACC TCTTTGGCTC GCAGTTTTGC TTTCTGTTAC
    AATCCAGCGC TACAGCCTAG AGCCCTTATA GTTTACGGGT GCATCAGCAA GAGTGTTACC
    GATCACGAGG TCAAGCAGTT ACTACGCATC CTGGTTAAGG CCCTCGAATC TTTCAACGAT
    CTCATTCTGA TCGAGGCTCT AGTAATGTGC TTAACTCGCA TTCAACCACT ACTCCGACCA
    GAGTCGCCCA TTCATCGGGC CCTCTTCTGG GTGGCCATTT CGGTGCTGCA GTTGGACGAG
    ATTACCCTGT ACGGCGCTGG ACTAGCTCTG CTTGAACAAA ATCTACACAC GCTTAAGTCA
    CAGGGCTGTT TTGACAAGAA GGAGACCATA GCCGAGGTCA TGATGAAGAC AAGGGAAAAG
    CTGGAGTGGC ATTTCAAGCA ACTGGATCAC GCCGTCGGCC TCTCCTTCCG CAGCAATTTC
    CACTTTGCCC TAGTGGGACA CTTGATTAAG GGCTTCCGCC ATCCTACACC TACAACCGTC
    TCACGCACCT CTCGTGTGCT GACGATGCTC TTGGGCATAA TAGCCAAACC CCTGCATCGG
    GACAAGTTCG AAGTAACACC TGACAGTGTG GCCTATTTGA CTGCGCTGGT GGCTGTCTCC
    GAGGAAGTCC GTTCCAGATG TCACGTAAAA CATGCCCTTC CCCGCTGGCC AGCCGATCTG
    AGTAGTAGTG TGGAAAATGG TGAAGCATCC GGCGGAGTGC AAGCTATCGG CCTGCCCCTG
    TCACGCCGTC AAAAAAGTTG GGACATCCTG GATCAGTCCG CCTTGCAGTT TGCCCGCCAA
    CACAAGGTTC CCACTCTTCA GAACGCGCGA GTTTTGTTCA AAACTCAACG CTCATTCTCG
    GTGCCGACGA CCAAAGATCC GAACAATGCC ACCGGCATCG AAGAACGTCA GGAACGCGGT
    TCGCGTTCGT CGGTGTCCAA CGAGTCCAAC GTACTGCTCG ATCCCGAGGT CTTACCTGAC
    CTGTCCATTC AAGCCCTGGT ACTGACTGTT TTGGCCACCT TGGTCAAGTA CTCTTCGGAT
    GAAGGCGAAA CGCGGGTTTT GTATCAGTAT TTGGCCGAAG GATCGGTGGT CTTTCCAAAG
    GTGTTTCCAG TTATCCATTC ACTGCTGGAC CAAAAGATAA ACAACATTTT GTCAGTGTCA
    CACGACCAAG TGGTGCTCAA CTCTGTGCAG AACATTATAC AAAATATGCT GGCCAGCGAA
    GATCCTTCCC AGCAGCAGCT GCACTTCCTG CAGAGTTGTG GATTTGGCGG ACTCTGGCGA
    TTTGCTGGTC CCTTCACCAA GTACAATATG ATGGGTGAGT CCTCGGAGCT GTTTGTCAAC
    TGTTTGGAGG CCATGGTTGA GACCTGTTTG CCAGGCGACG AATCCGCGCC GGTGCCACCA
    TCTCCGCGTC CATACAACCT GAGCTCCAGT CTGAGTAGCC TGACCCTGGG ATCACCCACG
    GATAAAGCTG CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa06161
    Clone ID Related Accession (Same CDS sequence) NM_170252.3
    Accession Version NM_170252.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product neurofibromin 1, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_170252.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGACCCAGA AGCCAGGCGA GTGGGCTAGC GCCCTTTTGG CACGTTTCGA GGATCAGCTG 
    CCAAACAGAA TCGGTGCCTA TGGCACGCAG GCCAGGATGA GCCAGGACCA ATTGGTGGCC
    TGTCTGATCC ACATATCGCG CTACCGCTTC TCCCTTGTCA TATCCGGTCT CACCAAGATG
    CTGCAGAGGG TCAATGAGGC GGCCCTTCAA AATCGCCATG AGCCAGAGCG TTGCTACTTT
    GAGTCGCTGG TCATCATACT GACCACTCTG GAACGCTGTC TGACCAACCA GACCAAGGAC
    ACAGCACGTT TTGAAGAGGC CATGAATGTG AAGCTTCTGC TGCGCGAGAT CTCCCAGTTT
    GTCGACGTCC AGAGCGACAG CAATCCGAAT GCCGCCCAGC TCAAGGCCCT GGCATCCAAG
    GTGCTCTTTG CCTTGTCCCA GAATCACTTC TCGGCGGTGT TCAATCGCAT ATCGGCCAGG
    ATTCAAGAGC TGACATCCTG TTCCGAGGAA AACCCGGACT ACAACGACAT AGAATTAATT
    CAACACATCG ATATGGATAT GATAAAGCTA ACCAAGCTGC TACAAGAAAC CATCACAAAG
    TTTCGATCGA AGCGTGCTCC ACCTTTGATC TTGCTTTACT CGCTGGAGAA GGCTATTTGG
    AACTGGATCG AGTACCATCC ACAAGAGTTC CAGGACTTGC AACGAGGAAC CAATCGAGAT
    ATATCAACCT GCTGGGAACC CCTGATGGAC TTTGTAGAGT ACTTTAAAAC CGAAAACAAG
    AAAAGCAAGA CTCTTGTTTG GCCGCTTCAA ATGCTCTTGC TGATATTGAA TCCCTCTTGC
    CTTGAGGCTG TTGTTAACGA ACTTCAGCAG TCGGAGAAAG AGAAGGAAAA GGACAAGGAA
    AAGGTTGCCT CGAAGTCAGC GCAATCCACG TCCCGGGACA AGGACTTCTC GGCCAAGCAG
    TTCATCGAGA GTATCAAGAG AGGCTTGGGC CAGCACTCAC CATCCAAACA GGTGACCGAA
    TCTGCGGCAA TTGCCTGTGT AAAGCTGTGC AAAGCATCCA CCTACATCAA CAACACCGAC
    TCCAATAATG TGGTCTTCAA GTTGGTGCAA TTCTTTATCA ACGATCTCAA GGCACTGCTC
    TTTAATCCAG CAAAGCCCTT TTCGCGCGGT CAGGGGTACA ACTTCGCTGA TATCGAGCTG
    ATGATTGACT GCTGGGTGTC TTGTTTCCGG ATTAATCCTC ACAACATCGA GGCTCTAAAG
    GTTTGCCTGA ATTTGTCCTC GCCGCAGGCT TACCATTTTG TAATTGTGTG CTCTCTGCTA
    AGGTTGGCTC ACATATACGT TGACTTCCGT TTGCAGAACA AGAACCCCTT TCGAATAGTA
    AACCAACCGA GATTGTCTTG GTGGCCGCAA ACGGATGTGG TTCACTACCG TTCTGCTGAG
    CTACGAGCTT TGTTTACGGA TACGCTCAAC AAGGCCACCC AGGGTTATAT AGCCCATACG
    CCACTGCGCT ACATAACCTC CCTGACGCTC AAATCTAAAG ATACCCAGAA AGGTTTAACC
    CGCGCTGAAG AGGGTCCCGC CCACAAAATG CTATTACTGC TACTCGTACG GCTAATTCAT
    GCAGATCCCA CGCTTTTGTT GAATACCCAA GGAAAAGTGG CACATGAAGT GCAGAGCTCC
    ACATTGGAGC TCATCAACGG CTTAGTTAGT TTGGTGCATC AAACTACCAT GCCCGATGTG
    GCACAAGAGG CTATGGAGGC GCTGCTTGCT CTGCACGCCC CGGAGAAAAT AGAGGTTTGG
    AATCCAGAGG CTCCGATCAA TACGTTCTGG GACGTTAGCT CCCAAGTACT GTTTTCAATC
    TCGCAAAAGC TCATTCAACA CCAAATTGCC AATTACACTG ATGTCTTAAA GTGGCTACGC
    GAGATCCTCA TCTGTCGGAA TACTTTTCTT CAGCGACACA AGGATTATGC ACATGTGGGA
    AGTCAGATTG CCATTTGCAA GCAGGCTCAC ATTAAGATGG AAGTTGTTTT CTTCATGTAC
    CTGTGGAGTG TTGATTTAGA TGCGGTGCTG ACATCGCTAT CGTGTTTTGG ACTACTATGC
    GAGGAGGCTG AGATTTGCTG CAGTTCTGAT GAATTGACAG TCGGATTTAT TATGCCGAAT
    TACCACATTT ACCAGGAGCT GGCTCAGTTG TCCACATCTG CAACGGACTC TCGCATTTGC
    TTTTTTGACA ACACGCACGG CAATGTGCTG AGCCGTCTTA CACTTCAAAA GCGCATAATG
    ACCCTATTGC GCAAGATCGA GCACTGTGTT CATGGTGTTC AGCCCGCCTG GGAGGAAACC
    TTCCGCAACT GGGAAGTATC CAGCAAGGTT TTGCAAACGT ACCCCAAATG TAAAGGAGAA
    GATGGCCAAG CGGAGGTTTT CCATCGTGGC ATGGGCAAGC GGCGAGCGAG TCACCAAAGC
    TCAGAGCACG ATTTGGAGGA ACAAATTAAT GAATGGGCCA ATATGACATG GTTTCTATTA
    GCCTTGGGTG GCGTCTGCCT TCATAAACGT AGCAGCAGTC GTCAAATGCT GCTGCAGCAA
    TCACAGAACA ATGCTTCTTT GGGATCACTT GCTCAAAACT CTCTTTACTC AAGCTCCACA
    AGTTCTGGAC ATGGCTCTCT GCATCCTAGC ACGGTTTCAC TATCCACTCT TCCCCCAGCA
    CCGCCACAAG ATGTCAGCTA TTGTCCTGTA ACACAATTCG TGGGCCAACT ATTGCGCCTG
    TTAGTTTGCA GCAACGAAAA AATTGGACTT AATATTCAGA AAAATGTGAA GGAACTGGTG
    GGCGAGGAGA TGTCCACCCA ACTGTATCCT ATACTCTTCG ACCAGGTTAG AGCCATCGTG
    GAGAAGTTCT TCGATCAGCA AGGTCAGGTG AACGTCAATG TGACCGACAT CAACACGCAG
    TTTATCGAGC ACACCATCTA CATAATGAAA TCAATTCTGG ATCCCAAAGC CAACAAGGAT
    CCCAACAACG ATCAACCCTC GCCATCGGAA CATTTAGGTG TTACAAGCAT CGAAGGCATG
    ATGCTAGGAA TAGTGCGCTA CGTTCGCCAC CTGGATATGA CTGTTTATGC CATTCGAATC
    AAGACAAAAT TGTGCCAGCT TGTGGAAGTA ATGATGAAGC GACGCGACGA CCTCGCCTTT
    CGCCAGGAGA TGAAGTTCCG GAATAAACTG GTTGAATACT TGACCGACTG GGTGATGGGC
    ACCTCCCATC AGATTGCACC GCCCAGCTCG GCGGATGCCG CTATTCTTAC CAACACATCC
    CTCATATTTC GCGATCTAGA CCAAGCCTGC ATGGAGGCAG TGGCTGCCTT GCTTCGGGGC
    CTTCCCCTTC AGCCTGAGGA ATCGGATCGA GGCGATTTGA TGGATGCAAA GAGTGCGCTC
    TTTTTAAAGT ACTTTACCCT ATTTATGAAC CTGCTGAATG ATTGCATCGA TAGCTCCGAG
    GCGGAGAAGG AAATGAATAA TACCCCACTA TTGCCTCCGC GCCCTCGAAT GGCAGCTGGA
    AAACTGACCG CTCTGCGAAA TGCCACTATT CTAGCCATGT CTAATTTGTT GGGTGCCAAC
    ATTGACTCTG GTTTGATGCA CTCCATCGAT CTGGGTTATA ATCCTGATTT GCAAACGCGT
    GCCGCTTTCA TGGAGGTGTT GACTCAAATC CTGCAACAAG GCACCGAATT CGATACCTTG
    GCCGAAACTG TTTTAGCTGA TCGATTTGAG CAACTGGTCC AACTTGTTAC AATGATCAGC
    GACAAAGGAG AACTTCCCAT AGCAATGGCT TTGGCTAATG TGGTGACTAC ATCCCAAATG
    GACGAGCTGG CTAGGGTTCT GGTCACCCTA TTCGACGCCA AACACCTGTT ATCGCCACTC
    CTATGGAATA TGTTTTATCG CGAGGTGGAG GTCTCAGACT GCATGCAGAC ACTTTTCCGT
    GGAAATTCTT TGGGCAGCAA AATCATGGCC TTTTGCTTTA AAATATACGG CGCGAGCTAT
    CTCCAAATGC TACTAGAGCC TTTAATTCGT CCGCTTCTGG ATGAGGAGGA GGAGACCTGT
    TTTGAGGTGG ATCCAGCTCG TCTGGATCCA ACAGAAGATA TTGAACAGCA CAGGAACAAC
    TTGATTGCCC TAACGCAGAA GGTGTTTGAC GCTATTATCA ACTCGTCGGA TCGCTTTCCC
    CCGCAGCTGC GATCCATGTG CCATTGCCTG TACCAAGTGC TAAGCAAACG CTTCCCGAAT
    CTTCTTCAAA ACAATATCGG TGCTGTAGGC ACAGTCATCT TCTTGCGGTT CATCAATCCC
    GCCATAGTTT CCCCACAGGA ATTGGGAATC GTTGACAAAC AAGTGCACAG CTCGGCCAAA
    CGAGGTCTAA TGCTAATGTC CAAGATCCTT CAAAACATTG CTAACCACGT GGAGTTCTCC
    AAGGAGCAGC ACATGTTGTG TTTCAACGAT TTTCTGCGCG ATCACTTTGA AGCTGGGCGG
    CGATTCTTCA TACAGATTGC ATCAGACTGT GAGACCGTGG ATCAGACCTC GCACAGCATG
    AGTTTTATTT CAGATGCGAA CGTACTGGCA CTGCATCGGT TGCTGTGGAC GCATCAGGAG
    AAGATCGGCG ACTATCTGTC TAGCAGTCGA GACCACAAGG CGGTTGGAAG GCGGCCCTTC
    GACAAGATGG CTACTTTGCT AGCATACCTG GGACCACCGG AGCACAAGCC CGTGGATTCG
    CACATGATGT TCAGCTCGTA TGCACGCTGG AGCTCCATTG ACATGTCGTC GACAAACTTC
    GAGGAGATCA TGGTCAAGCA CCAAATGCAC GAGAAGGAGG AATTCAAGAC CCTCAAGTCG
    ATGAACATAT TCTACCAGGC CGGAACAAGC AAATCTGGCT ATCCTGTCTT TTACTATATA
    GCAAGACGAT ACAAGATTGG AGAAACGAAT GGAGACTTAC TTATATATCA CGTCATACTC
    ACTCTGAAAC CATTCTGCCA CTCGCCCTTC GAGGTGGTCA TCGATTTTAC GCACACCTGT
    TCAGATAATC GGTTCCGCAC CGAGTTTCTG CAGAAGTGGT TTTATGTCCT GCCCACGGTA
    GCCTACGAAA ATGTCCATGC TGTGTACATC TATAACTGTA ACTCGTGGGT GCGCGAATAT
    ACCAAGTTCC ACGATCGCAT TCTGGCACCA TTAAAGGGAA ATCGCAAACT TCTGTTCCTG
    GAGTCACCCA ACAAACTTAC TGATTTCATC GACGCGGAGC AACAGAAATT GCCTGGAGCA
    ACTCTTTCCT TGGACGAAGA TTTAAAGGTA TTCAGCAACG CGCTGAAGCT CAGCCATAAA
    GACACAAAGG TGGCTATCAA AGTGGGTCCC ACCGCATTGC AAATTACATC TGCCGAAAAG
    ACAAAGGTCT TGGCTCACTC CGTGCTCCTG AACGATGTAT ACTACGCCTC TGAAATTGAG
    GAGGTGTGCT TGGTGGACGA TAACCAGTTC ACCCTGTCTA TAACTAACGA AAGTGGCCAG
    CTTAGCTTTA TTCACAACGA TTGCGACAAC ATTGTTCAAG CCATCATACA CATCCGAAAT
    CGATGGGAGC TTAGTCAGCC GGACTCCGTG ACGGTTCACC AAAAGATCCG ACCAAAAGAT
    GTGCCTGGAA CGCTGCTGAA CATGGCGCTA CTCAATCTGG GATCATGTGA CCCCAATCTA
    AGGACTGCTG CCTACAATTT GCTGTGTGCT TTGACCGCTA CTTTTGATCT AAAGATTGAG
    GGTCAACTGT TGGAGACCCA AGGACTTTGC ATACCCTCAA ATAATACCAT ATTTATCAAA
    TCCGTTAGCG AAAAGCTGGC CACCAATGAA CCGCATCTGA CTCTGGAATT CCTTGAGGAG
    TCCATTCAGG GCTTCCAGCG CAGCACCATA GAGCTGAAAC ATTTGTGTTT GGAGTACATG
    ACGCCATGGT TAAAGAACCT AGTCAAATTT TGCAAGTCCA ACGATGACTC CAAAAAGCTT
    AAGGTCTCCC AAATTCTGGA CAAGCTCATC AATTTAACTA TTGACCAAAA GGAAATGTAT
    CCCTCAGTGC AGGCCAAGAT CTGGGGATCA ATTGGACAGA TCCCCGAGCT GATCGACATG
    GTTTTGGATA ACTTTTTGCA CAAATCGATC ACATATGGCT TGGGATCACC ACAGGTGGAG
    ATTATGGCTG ACACGGCAGT GGCTCTCGCT TCAGCGAATG TTCAGCTGGT GTCCAAAAAG
    GTCATAACGC GAATATGCCG AGTCATGGAC AAATCCTGCA CAAATCCCAC ACAATATCTG
    GAGCAGCATA TGATGTGGGA CGATATTGCA ATTCTTGGTC GATACCTGCT CATGTTGTCG
    TTCAACAATT GTTTGGATGT GGCCACATCG GTGCCATATC TATTCCACAC CATTACGTTT
    TTGGTATGTT CAGGATCCCT GTCGATGCGA GCCTCTACTC ATGGCCTAGT GATCAACATC
    ATCCACTCGC TGTGCACATG CACAAATCCC TCATTTTCAG AGGAGGCGCA GCGAGTACTC
    CGACTGTCCC TGGATGAATT CTCACTGCCC AAGTTCTATC TACTCTTCGG CATCAGCAAG
    GTCAAGTCCG CAGCAGTTAC CGCTTTCCGC TCCAGCTGCC GTCACCCTAC AGATAAATGG
    CTAGGAAATG AAAGGGTTAC CCAGCCTCTG CCCGCCGATC GAGAGCGTTT ATCCCTACCA
    TCGCTTGAGG TTATCACGGA TGCCCTGCTG GAAATCATGG AGGCTTGCAT GCGAGATGTT
    CCGGACTGCG AATGGCTCAA CACCTGGACC TCTTTGGCTC GCAGTTTTGC TTTCTGTTAC
    AATCCAGCGC TACAGCCTAG AGCCCTTATA GTTTACGGGT GCATCAGCAA GAGTGTTACC
    GATCACGAGG TCAAGCAGTT ACTACGCATC CTGGTTAAGG CCCTCGAATC TTTCAACGAT
    CTCATTCTGA TCGAGGCTCT AGTAATGTGC TTAACTCGCA TTCAACCACT ACTCCGACCA
    GAGTCGCCCA TTCATCGGGC CCTCTTCTGG GTGGCCATTT CGGTGCTGCA GTTGGACGAG
    ATTACCCTGT ACGGCGCTGG ACTAGCTCTG CTTGAACAAA ATCTACACAC GCTTAAGTCA
    CAGGGCTGTT TTGACAAGAA GGAGACCATA GCCGAGGTCA TGATGAAGAC AAGGGAAAAG
    CTGGAGTGGC ATTTCAAGCA ACTGGATCAC GCCGTCGGCC TCTCCTTCCG CAGCAATTTC
    CACTTTGCCC TAGTGGGACA CTTGATTAAG GGCTTCCGCC ATCCTACACC TACAACCGTC
    TCACGCACCT CTCGTGTGCT GACGATGCTC TTGGGCATAA TAGCCAAACC CCTGCATCGG
    GACAAGTTCG AAGTAACACC TGACAGTGTG GCCTATTTGA CTGCGCTGGT GGCTGTCTCC
    GAGGAAGTCC GTTCCAGATG TCACGTAAAA CATGCCCTTC CCCGCTGGCC AGCCGATCTG
    AGTAGTAGTG TGGAAAATGG TGAAGCATCC GGCGGAGTGC AAGCTATCGG CCTGCCCCTG
    TCACGCCGTC AAAAAAGTTG GGACATCCTG GATCAGTCCG CCTTGCAGTT TGCCCGCCAA
    CACAAGGTTC CCACTCTTCA GGAACGCGGT TCGCGTTCGT CGGTGTCCAA CGAGTCCAAC
    GTACTGCTCG ATCCCGAGGT CTTACCTGAC CTGTCCATTC AAGCCCTGGT ACTGACTGTT
    TTGGCCACCT TGGTCAAGTA CTCTTCGGAT GAAGGCGAAA CGCGGGTTTT GTATCAGTAT
    TTGGCCGAAG GATCGGTGGT CTTTCCAAAG GTGTTTCCAG TTATCCATTC ACTGCTGGAC
    CAAAAGATAA ACAACATTTT GTCAGTGTCA CACGACCAAG TGGTGCTCAA CTCTGTGCAG
    AACATTATAC AAAATATGCT GGCCAGCGAA GATCCTTCCC AGCAGCAGCT GCACTTCCTG
    CAGAGTTGTG GATTTGGCGG ACTCTGGCGA TTTGCTGGTC CCTTCACCAA GTACAATATG
    ATGGGTGAGT CCTCGGAGCT GTTTGTCAAC TGTTTGGAGG CCATGGTTGA GACCTGTTTG
    CCAGGCGACG AATCCGCGCC GGTGCCACCA TCTCCGCGTC CATACAACCT GAGCTCCAGT
    CTGAGTAGCC TGACCCTGGG ATCACCCACG GATAAAGAAT GTCCAGAAGC GATGACCACT
    GAATGCGAAA GAAGGAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_733131.2
    CDS280..8520
    Translation

    Target ORF information:

    RefSeq Version NM_170252.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster neurofibromin 1, transcript variant C (Nf1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_170252.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGACCCAGA AGCCAGGCGA GTGGGCTAGC GCCCTTTTGG CACGTTTCGA GGATCAGCTG 
    CCAAACAGAA TCGGTGCCTA TGGCACGCAG GCCAGGATGA GCCAGGACCA ATTGGTGGCC
    TGTCTGATCC ACATATCGCG CTACCGCTTC TCCCTTGTCA TATCCGGTCT CACCAAGATG
    CTGCAGAGGG TCAATGAGGC GGCCCTTCAA AATCGCCATG AGCCAGAGCG TTGCTACTTT
    GAGTCGCTGG TCATCATACT GACCACTCTG GAACGCTGTC TGACCAACCA GACCAAGGAC
    ACAGCACGTT TTGAAGAGGC CATGAATGTG AAGCTTCTGC TGCGCGAGAT CTCCCAGTTT
    GTCGACGTCC AGAGCGACAG CAATCCGAAT GCCGCCCAGC TCAAGGCCCT GGCATCCAAG
    GTGCTCTTTG CCTTGTCCCA GAATCACTTC TCGGCGGTGT TCAATCGCAT ATCGGCCAGG
    ATTCAAGAGC TGACATCCTG TTCCGAGGAA AACCCGGACT ACAACGACAT AGAATTAATT
    CAACACATCG ATATGGATAT GATAAAGCTA ACCAAGCTGC TACAAGAAAC CATCACAAAG
    TTTCGATCGA AGCGTGCTCC ACCTTTGATC TTGCTTTACT CGCTGGAGAA GGCTATTTGG
    AACTGGATCG AGTACCATCC ACAAGAGTTC CAGGACTTGC AACGAGGAAC CAATCGAGAT
    ATATCAACCT GCTGGGAACC CCTGATGGAC TTTGTAGAGT ACTTTAAAAC CGAAAACAAG
    AAAAGCAAGA CTCTTGTTTG GCCGCTTCAA ATGCTCTTGC TGATATTGAA TCCCTCTTGC
    CTTGAGGCTG TTGTTAACGA ACTTCAGCAG TCGGAGAAAG AGAAGGAAAA GGACAAGGAA
    AAGGTTGCCT CGAAGTCAGC GCAATCCACG TCCCGGGACA AGGACTTCTC GGCCAAGCAG
    TTCATCGAGA GTATCAAGAG AGGCTTGGGC CAGCACTCAC CATCCAAACA GGTGACCGAA
    TCTGCGGCAA TTGCCTGTGT AAAGCTGTGC AAAGCATCCA CCTACATCAA CAACACCGAC
    TCCAATAATG TGGTCTTCAA GTTGGTGCAA TTCTTTATCA ACGATCTCAA GGCACTGCTC
    TTTAATCCAG CAAAGCCCTT TTCGCGCGGT CAGGGGTACA ACTTCGCTGA TATCGAGCTG
    ATGATTGACT GCTGGGTGTC TTGTTTCCGG ATTAATCCTC ACAACATCGA GGCTCTAAAG
    GTTTGCCTGA ATTTGTCCTC GCCGCAGGCT TACCATTTTG TAATTGTGTG CTCTCTGCTA
    AGGTTGGCTC ACATATACGT TGACTTCCGT TTGCAGAACA AGAACCCCTT TCGAATAGTA
    AACCAACCGA GATTGTCTTG GTGGCCGCAA ACGGATGTGG TTCACTACCG TTCTGCTGAG
    CTACGAGCTT TGTTTACGGA TACGCTCAAC AAGGCCACCC AGGGTTATAT AGCCCATACG
    CCACTGCGCT ACATAACCTC CCTGACGCTC AAATCTAAAG ATACCCAGAA AGGTTTAACC
    CGCGCTGAAG AGGGTCCCGC CCACAAAATG CTATTACTGC TACTCGTACG GCTAATTCAT
    GCAGATCCCA CGCTTTTGTT GAATACCCAA GGAAAAGTGG CACATGAAGT GCAGAGCTCC
    ACATTGGAGC TCATCAACGG CTTAGTTAGT TTGGTGCATC AAACTACCAT GCCCGATGTG
    GCACAAGAGG CTATGGAGGC GCTGCTTGCT CTGCACGCCC CGGAGAAAAT AGAGGTTTGG
    AATCCAGAGG CTCCGATCAA TACGTTCTGG GACGTTAGCT CCCAAGTACT GTTTTCAATC
    TCGCAAAAGC TCATTCAACA CCAAATTGCC AATTACACTG ATGTCTTAAA GTGGCTACGC
    GAGATCCTCA TCTGTCGGAA TACTTTTCTT CAGCGACACA AGGATTATGC ACATGTGGGA
    AGTCAGATTG CCATTTGCAA GCAGGCTCAC ATTAAGATGG AAGTTGTTTT CTTCATGTAC
    CTGTGGAGTG TTGATTTAGA TGCGGTGCTG ACATCGCTAT CGTGTTTTGG ACTACTATGC
    GAGGAGGCTG AGATTTGCTG CAGTTCTGAT GAATTGACAG TCGGATTTAT TATGCCGAAT
    TACCACATTT ACCAGGAGCT GGCTCAGTTG TCCACATCTG CAACGGACTC TCGCATTTGC
    TTTTTTGACA ACACGCACGG CAATGTGCTG AGCCGTCTTA CACTTCAAAA GCGCATAATG
    ACCCTATTGC GCAAGATCGA GCACTGTGTT CATGGTGTTC AGCCCGCCTG GGAGGAAACC
    TTCCGCAACT GGGAAGTATC CAGCAAGGTT TTGCAAACGT ACCCCAAATG TAAAGGAGAA
    GATGGCCAAG CGGAGGTTTT CCATCGTGGC ATGGGCAAGC GGCGAGCGAG TCACCAAAGC
    TCAGAGCACG ATTTGGAGGA ACAAATTAAT GAATGGGCCA ATATGACATG GTTTCTATTA
    GCCTTGGGTG GCGTCTGCCT TCATAAACGT AGCAGCAGTC GTCAAATGCT GCTGCAGCAA
    TCACAGAACA ATGCTTCTTT GGGATCACTT GCTCAAAACT CTCTTTACTC AAGCTCCACA
    AGTTCTGGAC ATGGCTCTCT GCATCCTAGC ACGGTTTCAC TATCCACTCT TCCCCCAGCA
    CCGCCACAAG ATGTCAGCTA TTGTCCTGTA ACACAATTCG TGGGCCAACT ATTGCGCCTG
    TTAGTTTGCA GCAACGAAAA AATTGGACTT AATATTCAGA AAAATGTGAA GGAACTGGTG
    GGCGAGGAGA TGTCCACCCA ACTGTATCCT ATACTCTTCG ACCAGGTTAG AGCCATCGTG
    GAGAAGTTCT TCGATCAGCA AGGTCAGGTG AACGTCAATG TGACCGACAT CAACACGCAG
    TTTATCGAGC ACACCATCTA CATAATGAAA TCAATTCTGG ATCCCAAAGC CAACAAGGAT
    CCCAACAACG ATCAACCCTC GCCATCGGAA CATTTAGGTG TTACAAGCAT CGAAGGCATG
    ATGCTAGGAA TAGTGCGCTA CGTTCGCCAC CTGGATATGA CTGTTTATGC CATTCGAATC
    AAGACAAAAT TGTGCCAGCT TGTGGAAGTA ATGATGAAGC GACGCGACGA CCTCGCCTTT
    CGCCAGGAGA TGAAGTTCCG GAATAAACTG GTTGAATACT TGACCGACTG GGTGATGGGC
    ACCTCCCATC AGATTGCACC GCCCAGCTCG GCGGATGCCG CTATTCTTAC CAACACATCC
    CTCATATTTC GCGATCTAGA CCAAGCCTGC ATGGAGGCAG TGGCTGCCTT GCTTCGGGGC
    CTTCCCCTTC AGCCTGAGGA ATCGGATCGA GGCGATTTGA TGGATGCAAA GAGTGCGCTC
    TTTTTAAAGT ACTTTACCCT ATTTATGAAC CTGCTGAATG ATTGCATCGA TAGCTCCGAG
    GCGGAGAAGG AAATGAATAA TACCCCACTA TTGCCTCCGC GCCCTCGAAT GGCAGCTGGA
    AAACTGACCG CTCTGCGAAA TGCCACTATT CTAGCCATGT CTAATTTGTT GGGTGCCAAC
    ATTGACTCTG GTTTGATGCA CTCCATCGAT CTGGGTTATA ATCCTGATTT GCAAACGCGT
    GCCGCTTTCA TGGAGGTGTT GACTCAAATC CTGCAACAAG GCACCGAATT CGATACCTTG
    GCCGAAACTG TTTTAGCTGA TCGATTTGAG CAACTGGTCC AACTTGTTAC AATGATCAGC
    GACAAAGGAG AACTTCCCAT AGCAATGGCT TTGGCTAATG TGGTGACTAC ATCCCAAATG
    GACGAGCTGG CTAGGGTTCT GGTCACCCTA TTCGACGCCA AACACCTGTT ATCGCCACTC
    CTATGGAATA TGTTTTATCG CGAGGTGGAG GTCTCAGACT GCATGCAGAC ACTTTTCCGT
    GGAAATTCTT TGGGCAGCAA AATCATGGCC TTTTGCTTTA AAATATACGG CGCGAGCTAT
    CTCCAAATGC TACTAGAGCC TTTAATTCGT CCGCTTCTGG ATGAGGAGGA GGAGACCTGT
    TTTGAGGTGG ATCCAGCTCG TCTGGATCCA ACAGAAGATA TTGAACAGCA CAGGAACAAC
    TTGATTGCCC TAACGCAGAA GGTGTTTGAC GCTATTATCA ACTCGTCGGA TCGCTTTCCC
    CCGCAGCTGC GATCCATGTG CCATTGCCTG TACCAAGTGC TAAGCAAACG CTTCCCGAAT
    CTTCTTCAAA ACAATATCGG TGCTGTAGGC ACAGTCATCT TCTTGCGGTT CATCAATCCC
    GCCATAGTTT CCCCACAGGA ATTGGGAATC GTTGACAAAC AAGTGCACAG CTCGGCCAAA
    CGAGGTCTAA TGCTAATGTC CAAGATCCTT CAAAACATTG CTAACCACGT GGAGTTCTCC
    AAGGAGCAGC ACATGTTGTG TTTCAACGAT TTTCTGCGCG ATCACTTTGA AGCTGGGCGG
    CGATTCTTCA TACAGATTGC ATCAGACTGT GAGACCGTGG ATCAGACCTC GCACAGCATG
    AGTTTTATTT CAGATGCGAA CGTACTGGCA CTGCATCGGT TGCTGTGGAC GCATCAGGAG
    AAGATCGGCG ACTATCTGTC TAGCAGTCGA GACCACAAGG CGGTTGGAAG GCGGCCCTTC
    GACAAGATGG CTACTTTGCT AGCATACCTG GGACCACCGG AGCACAAGCC CGTGGATTCG
    CACATGATGT TCAGCTCGTA TGCACGCTGG AGCTCCATTG ACATGTCGTC GACAAACTTC
    GAGGAGATCA TGGTCAAGCA CCAAATGCAC GAGAAGGAGG AATTCAAGAC CCTCAAGTCG
    ATGAACATAT TCTACCAGGC CGGAACAAGC AAATCTGGCT ATCCTGTCTT TTACTATATA
    GCAAGACGAT ACAAGATTGG AGAAACGAAT GGAGACTTAC TTATATATCA CGTCATACTC
    ACTCTGAAAC CATTCTGCCA CTCGCCCTTC GAGGTGGTCA TCGATTTTAC GCACACCTGT
    TCAGATAATC GGTTCCGCAC CGAGTTTCTG CAGAAGTGGT TTTATGTCCT GCCCACGGTA
    GCCTACGAAA ATGTCCATGC TGTGTACATC TATAACTGTA ACTCGTGGGT GCGCGAATAT
    ACCAAGTTCC ACGATCGCAT TCTGGCACCA TTAAAGGGAA ATCGCAAACT TCTGTTCCTG
    GAGTCACCCA ACAAACTTAC TGATTTCATC GACGCGGAGC AACAGAAATT GCCTGGAGCA
    ACTCTTTCCT TGGACGAAGA TTTAAAGGTA TTCAGCAACG CGCTGAAGCT CAGCCATAAA
    GACACAAAGG TGGCTATCAA AGTGGGTCCC ACCGCATTGC AAATTACATC TGCCGAAAAG
    ACAAAGGTCT TGGCTCACTC CGTGCTCCTG AACGATGTAT ACTACGCCTC TGAAATTGAG
    GAGGTGTGCT TGGTGGACGA TAACCAGTTC ACCCTGTCTA TAACTAACGA AAGTGGCCAG
    CTTAGCTTTA TTCACAACGA TTGCGACAAC ATTGTTCAAG CCATCATACA CATCCGAAAT
    CGATGGGAGC TTAGTCAGCC GGACTCCGTG ACGGTTCACC AAAAGATCCG ACCAAAAGAT
    GTGCCTGGAA CGCTGCTGAA CATGGCGCTA CTCAATCTGG GATCATGTGA CCCCAATCTA
    AGGACTGCTG CCTACAATTT GCTGTGTGCT TTGACCGCTA CTTTTGATCT AAAGATTGAG
    GGTCAACTGT TGGAGACCCA AGGACTTTGC ATACCCTCAA ATAATACCAT ATTTATCAAA
    TCCGTTAGCG AAAAGCTGGC CACCAATGAA CCGCATCTGA CTCTGGAATT CCTTGAGGAG
    TCCATTCAGG GCTTCCAGCG CAGCACCATA GAGCTGAAAC ATTTGTGTTT GGAGTACATG
    ACGCCATGGT TAAAGAACCT AGTCAAATTT TGCAAGTCCA ACGATGACTC CAAAAAGCTT
    AAGGTCTCCC AAATTCTGGA CAAGCTCATC AATTTAACTA TTGACCAAAA GGAAATGTAT
    CCCTCAGTGC AGGCCAAGAT CTGGGGATCA ATTGGACAGA TCCCCGAGCT GATCGACATG
    GTTTTGGATA ACTTTTTGCA CAAATCGATC ACATATGGCT TGGGATCACC ACAGGTGGAG
    ATTATGGCTG ACACGGCAGT GGCTCTCGCT TCAGCGAATG TTCAGCTGGT GTCCAAAAAG
    GTCATAACGC GAATATGCCG AGTCATGGAC AAATCCTGCA CAAATCCCAC ACAATATCTG
    GAGCAGCATA TGATGTGGGA CGATATTGCA ATTCTTGGTC GATACCTGCT CATGTTGTCG
    TTCAACAATT GTTTGGATGT GGCCACATCG GTGCCATATC TATTCCACAC CATTACGTTT
    TTGGTATGTT CAGGATCCCT GTCGATGCGA GCCTCTACTC ATGGCCTAGT GATCAACATC
    ATCCACTCGC TGTGCACATG CACAAATCCC TCATTTTCAG AGGAGGCGCA GCGAGTACTC
    CGACTGTCCC TGGATGAATT CTCACTGCCC AAGTTCTATC TACTCTTCGG CATCAGCAAG
    GTCAAGTCCG CAGCAGTTAC CGCTTTCCGC TCCAGCTGCC GTCACCCTAC AGATAAATGG
    CTAGGAAATG AAAGGGTTAC CCAGCCTCTG CCCGCCGATC GAGAGCGTTT ATCCCTACCA
    TCGCTTGAGG TTATCACGGA TGCCCTGCTG GAAATCATGG AGGCTTGCAT GCGAGATGTT
    CCGGACTGCG AATGGCTCAA CACCTGGACC TCTTTGGCTC GCAGTTTTGC TTTCTGTTAC
    AATCCAGCGC TACAGCCTAG AGCCCTTATA GTTTACGGGT GCATCAGCAA GAGTGTTACC
    GATCACGAGG TCAAGCAGTT ACTACGCATC CTGGTTAAGG CCCTCGAATC TTTCAACGAT
    CTCATTCTGA TCGAGGCTCT AGTAATGTGC TTAACTCGCA TTCAACCACT ACTCCGACCA
    GAGTCGCCCA TTCATCGGGC CCTCTTCTGG GTGGCCATTT CGGTGCTGCA GTTGGACGAG
    ATTACCCTGT ACGGCGCTGG ACTAGCTCTG CTTGAACAAA ATCTACACAC GCTTAAGTCA
    CAGGGCTGTT TTGACAAGAA GGAGACCATA GCCGAGGTCA TGATGAAGAC AAGGGAAAAG
    CTGGAGTGGC ATTTCAAGCA ACTGGATCAC GCCGTCGGCC TCTCCTTCCG CAGCAATTTC
    CACTTTGCCC TAGTGGGACA CTTGATTAAG GGCTTCCGCC ATCCTACACC TACAACCGTC
    TCACGCACCT CTCGTGTGCT GACGATGCTC TTGGGCATAA TAGCCAAACC CCTGCATCGG
    GACAAGTTCG AAGTAACACC TGACAGTGTG GCCTATTTGA CTGCGCTGGT GGCTGTCTCC
    GAGGAAGTCC GTTCCAGATG TCACGTAAAA CATGCCCTTC CCCGCTGGCC AGCCGATCTG
    AGTAGTAGTG TGGAAAATGG TGAAGCATCC GGCGGAGTGC AAGCTATCGG CCTGCCCCTG
    TCACGCCGTC AAAAAAGTTG GGACATCCTG GATCAGTCCG CCTTGCAGTT TGCCCGCCAA
    CACAAGGTTC CCACTCTTCA GGAACGCGGT TCGCGTTCGT CGGTGTCCAA CGAGTCCAAC
    GTACTGCTCG ATCCCGAGGT CTTACCTGAC CTGTCCATTC AAGCCCTGGT ACTGACTGTT
    TTGGCCACCT TGGTCAAGTA CTCTTCGGAT GAAGGCGAAA CGCGGGTTTT GTATCAGTAT
    TTGGCCGAAG GATCGGTGGT CTTTCCAAAG GTGTTTCCAG TTATCCATTC ACTGCTGGAC
    CAAAAGATAA ACAACATTTT GTCAGTGTCA CACGACCAAG TGGTGCTCAA CTCTGTGCAG
    AACATTATAC AAAATATGCT GGCCAGCGAA GATCCTTCCC AGCAGCAGCT GCACTTCCTG
    CAGAGTTGTG GATTTGGCGG ACTCTGGCGA TTTGCTGGTC CCTTCACCAA GTACAATATG
    ATGGGTGAGT CCTCGGAGCT GTTTGTCAAC TGTTTGGAGG CCATGGTTGA GACCTGTTTG
    CCAGGCGACG AATCCGCGCC GGTGCCACCA TCTCCGCGTC CATACAACCT GAGCTCCAGT
    CTGAGTAGCC TGACCCTGGG ATCACCCACG GATAAAGAAT GTCCAGAAGC GATGACCACT
    GAATGCGAAA GAAGGAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa06162
    Clone ID Related Accession (Same CDS sequence) NM_170253.2
    Accession Version NM_170253.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8409bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product neurofibromin 1, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Mar 18, 2004 this sequence version replaced NM_170253.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGACCCAGA AGCCAGGCGA GTGGGCTAGC GCCCTTTTGG CACGTTTCGA GGATCAGCTG 
    CCAAACAGAA TCGGTGCCTA TGGCACGCAG GCCAGGATGA GCCAGGACCA ATTGGTGGCC
    TGTCTGATCC ACATATCGCG CTACCGCTTC TCCCTTGTCA TATCCGGTCT CACCAAGATG
    CTGCAGAGGG TCAATGAGGC GGCCCTTCAA AATCGCCATG AGCCAGAGCG TTGCTACTTT
    GAGTCGCTGG TCATCATACT GACCACTCTG GAACGCTGTC TGACCAACCA GACCAAGGAC
    ACAGCACGTT TTGAAGAGGC CATGAATGTG AAGCTTCTGC TGCGCGAGAT CTCCCAGTTT
    GTCGACGTCC AGAGCGACAG CAATCCGAAT GCCGCCCAGC TCAAGGCCCT GGCATCCAAG
    GTGCTCTTTG CCTTGTCCCA GAATCACTTC TCGGCGGTGT TCAATCGCAT ATCGGCCAGG
    ATTCAAGAGC TGACATCCTG TTCCGAGGAA AACCCGGACT ACAACGACAT AGAATTAATT
    CAACACATCG ATATGGATAT GATAAAGCTA ACCAAGCTGC TACAAGAAAC CATCACAAAG
    TTTCGATCGA AGCGTGCTCC ACCTTTGATC TTGCTTTACT CGCTGGAGAA GGCTATTTGG
    AACTGGATCG AGTACCATCC ACAAGAGTTC CAGGACTTGC AACGAGGAAC CAATCGAGAT
    ATATCAACCT GCTGGGAACC CCTGATGGAC TTTGTAGAGT ACTTTAAAAC CGAAAACAAG
    AAAAGCAAGA CTCTTGTTTG GCCGCTTCAA ATGCTCTTGC TGATATTGAA TCCCTCTTGC
    CTTGAGGCTG TTGTTAACGA ACTTCAGCAG TCGGAGAAAG AGAAGGAAAA GGACAAGGAA
    AAGGTTGCCT CGAAGTCAGC GCAATCCACG TCCCGGGACA AGGACTTCTC GGCCAAGCAG
    TTCATCGAGA GTATCAAGAG AGGCTTGGGC CAGCACTCAC CATCCAAACA GGTGACCGAA
    TCTGCGGCAA TTGCCTGTGT AAAGCTGTGC AAAGCATCCA CCTACATCAA CAACACCGAC
    TCCAATAATG TGGTCTTCAA GTTGGTGCAA TTCTTTATCA ACGATCTCAA GGCACTGCTC
    TTTAATCCAG CAAAGCCCTT TTCGCGCGGT CAGGGGTACA ACTTCGCTGA TATCGAGCTG
    ATGATTGACT GCTGGGTGTC TTGTTTCCGG ATTAATCCTC ACAACATCGA GGCTCTAAAG
    GTTTGCCTGA ATTTGTCCTC GCCGCAGGCT TACCATTTTG TAATTGTGTG CTCTCTGCTA
    AGGTTGGCTC ACATATACGT TGACTTCCGT TTGCAGAACA AGAACCCCTT TCGAATAGTA
    AACCAACCGA GATTGTCTTG GTGGCCGCAA ACGGATGTGG TTCACTACCG TTCTGCTGAG
    CTACGAGCTT TGTTTACGGA TACGCTCAAC AAGGCCACCC AGGGTTATAT AGCCCATACG
    CCACTGCGCT ACATAACCTC CCTGACGCTC AAATCTAAAG ATACCCAGAA AGGTTTAACC
    CGCGCTGAAG AGGGTCCCGC CCACAAAATG CTATTACTGC TACTCGTACG GCTAATTCAT
    GCAGATCCCA CGCTTTTGTT GAATACCCAA GGAAAAGTGG CACATGAAGT GCAGAGCTCC
    ACATTGGAGC TCATCAACGG CTTAGTTAGT TTGGTGCATC AAACTACCAT GCCCGATGTG
    GCACAAGAGG CTATGGAGGC GCTGCTTGCT CTGCACGCCC CGGAGAAAAT AGAGGTTTGG
    AATCCAGAGG CTCCGATCAA TACGTTCTGG GACGTTAGCT CCCAAGTACT GTTTTCAATC
    TCGCAAAAGC TCATTCAACA CCAAATTGCC AATTACACTG ATGTCTTAAA GTGGCTACGC
    GAGATCCTCA TCTGTCGGAA TACTTTTCTT CAGCGACACA AGGATTATGC ACATGTGGGA
    AGTCAGATTG CCATTTGCAA GCAGGCTCAC ATTAAGATGG AAGTTGTTTT CTTCATGTAC
    CTGTGGAGTG TTGATTTAGA TGCGGTGCTG ACATCGCTAT CGTGTTTTGG ACTACTATGC
    GAGGAGGCTG AGATTTGCTG CAGTTCTGAT GAATTGACAG TCGGATTTAT TATGCCGAAT
    TACCACATTT ACCAGGAGCT GGCTCAGTTG TCCACATCTG CAACGGACTC TCGCATTTGC
    TTTTTTGACA ACACGCACGG CAATGTGCTG AGCCGTCTTA CACTTCAAAA GCGCATAATG
    ACCCTATTGC GCAAGATCGA GCACTGTGTT CATGGTGTTC AGCCCGCCTG GGAGGAAACC
    TTCCGCAACT GGGAAGTATC CAGCAAGGTT TTGCAAACGT ACCCCAAATG TAAAGGAGAA
    GATGGCCAAG CGGAGGTTTT CCATCGTGGC ATGGGCAAGC GGCGAGCGAG TCACCAAAGC
    TCAGAGCACG ATTTGGAGGA ACAAATTAAT GAATGGGCCA ATATGACATG GTTTCTATTA
    GCCTTGGGTG GCGTCTGCCT TCATAAACGT AGCAGCAGTC GTCAAATGCT GCTGCAGCAA
    TCACAGAACA ATGCTTCTTT GGGATCACTT GCTCAAAACT CTCTTTACTC AAGCTCCACA
    AGTTCTGGAC ATGGCTCTCT GCATCCTAGC ACGGTTTCAC TATCCACTCT TCCCCCAGCA
    CCGCCACAAG ATGTCAGCTA TTGTCCTGTA ACACAATTCG TGGGCCAACT ATTGCGCCTG
    TTAGTTTGCA GCAACGAAAA AATTGGACTT AATATTCAGA AAAATGTGAA GGAACTGGTG
    GGCGAGGAGA TGTCCACCCA ACTGTATCCT ATACTCTTCG ACCAGGTTAG AGCCATCGTG
    GAGAAGTTCT TCGATCAGCA AGGTCAGGTG AACGTCAATG TGACCGACAT CAACACGCAG
    TTTATCGAGC ACACCATCTA CATAATGAAA TCAATTCTGG ATCCCAAAGC CAACAAGGAT
    CCCAACAACG ATCAACCCTC GCCATCGGAA CATTTAGGTG TTACAAGCAT CGAAGGCATG
    ATGCTAGGAA TAGTGCGCTA CGTTCGCCAC CTGGATATGA CTGTTTATGC CATTCGAATC
    AAGACAAAAT TGTGCCAGCT TGTGGAAGTA ATGATGAAGC GACGCGACGA CCTCGCCTTT
    CGCCAGGAGA TGAAGTTCCG GAATAAACTG GTTGAATACT TGACCGACTG GGTGATGGGC
    ACCTCCCATC AGATTGCACC GCCCAGCTCG GCGGATGCCG CTATTCTTAC CAACACATCC
    CTCATATTTC GCGATCTAGA CCAAGCCTGC ATGGAGGCAG TGGCTGCCTT GCTTCGGGGC
    CTTCCCCTTC AGCCTGAGGA ATCGGATCGA GGCGATTTGA TGGATGCAAA GAGTGCGCTC
    TTTTTAAAGT ACTTTACCCT ATTTATGAAC CTGCTGAATG ATTGCATCGA TAGCTCCGAG
    GCGGAGAAGG AAATGAATAA TACCCCACTA TTGCCTCCGC GCCCTCGAAT GGCAGCTGGA
    AAACTGACCG CTCTGCGAAA TGCCACTATT CTAGCCATGT CTAATTTGTT GGGTGCCAAC
    ATTGACTCTG GTTTGATGCA CTCCATCGAT CTGGGTTATA ATCCTGATTT GCAAACGCGT
    GCCGCTTTCA TGGAGGTGTT GACTCAAATC CTGCAACAAG GCACCGAATT CGATACCTTG
    GCCGAAACTG TTTTAGCTGA TCGATTTGAG CAACTGGTCC AACTTGTTAC AATGATCAGC
    GACAAAGGAG AACTTCCCAT AGCAATGGCT TTGGCTAATG TGGTGACTAC ATCCCAAATG
    GACGAGCTGG CTAGGGTTCT GGTCACCCTA TTCGACGCCA AACACCTGTT ATCGCCACTC
    CTATGGAATA TGTTTTATCG CGAGGTGGAG GTCTCAGACT GCATGCAGAC ACTTTTCCGT
    GGAAATTCTT TGGGCAGCAA AATCATGGCC TTTTGCTTTA AAATATACGG CGCGAGCTAT
    CTCCAAATGC TACTAGAGCC TTTAATTCGT CCGCTTCTGG ATGAGGAGGA GGAGACCTGT
    TTTGAGGTGG ATCCAGCTCG TCTGGATCCA ACAGAAGATA TTGAACAGCA CAGGAACAAC
    TTGATTGCCC TAACGCAGAA GGTGTTTGAC GCTATTATCA ACTCGTCGGA TCGCTTTCCC
    CCGCAGCTGC GATCCATGTG CCATTGCCTG TACCAAGTGC TAAGCAAACG CTTCCCGAAT
    CTTCTTCAAA ACAATATCGG TGCTGTAGGC ACAGTCATCT TCTTGCGGTT CATCAATCCC
    GCCATAGTTT CCCCACAGGA ATTGGGAATC GTTGACAAAC AAGTGCACAG CTCGGCCAAA
    CGAGGTCTAA TGCTAATGTC CAAGATCCTT CAAAACATTG CTAACCACGT GGAGTTCTCC
    AAGGAGCAGC ACATGTTGTG TTTCAACGAT TTTCTGCGCG ATCACTTTGA AGCTGGGCGG
    CGATTCTTCA TACAGATTGC ATCAGACTGT GAGACCGTGG ATCAGACCTC GCACAGCATG
    AGTTTTATTT CAGATGCGAA CGTACTGGCA CTGCATCGGT TGCTGTGGAC GCATCAGGAG
    AAGATCGGCG ACTATCTGTC TAGCAGTCGA GACCACAAGG CGGTTGGAAG GCGGCCCTTC
    GACAAGATGG CTACTTTGCT AGCATACCTG GGACCACCGG AGCACAAGCC CGTGGATTCG
    CACATGATGT TCAGCTCGTA TGCACGCTGG AGCTCCATTG ACATGTCGTC GACAAACTTC
    GAGGAGATCA TGGTCAAGCA CCAAATGCAC GAGAAGGAGG AATTCAAGAC CCTCAAGTCG
    ATGAACATAT TCTACCAGGC CGGAACAAGC AAATCTGGCT ATCCTGTCTT TTACTATATA
    GCAAGACGAT ACAAGATTGG AGAAACGAAT GGAGACTTAC TTATATATCA CGTCATACTC
    ACTCTGAAAC CATTCTGCCA CTCGCCCTTC GAGGTGGTCA TCGATTTTAC GCACACCTGT
    TCAGATAATC GGTTCCGCAC CGAGTTTCTG CAGAAGTGGT TTTATGTCCT GCCCACGGTA
    GCCTACGAAA ATGTCCATGC TGTGTACATC TATAACTGTA ACTCGTGGGT GCGCGAATAT
    ACCAAGTTCC ACGATCGCAT TCTGGCACCA TTAAAGGGAA ATCGCAAACT TCTGTTCCTG
    GAGTCACCCA ACAAACTTAC TGATTTCATC GACGCGGAGC AACAGAAATT GCCTGGAGCA
    ACTCTTTCCT TGGACGAAGA TTTAAAGGTA TTCAGCAACG CGCTGAAGCT CAGCCATAAA
    GACACAAAGG TGGCTATCAA AGTGGGTCCC ACCGCATTGC AAATTACATC TGCCGAAAAG
    ACAAAGGTCT TGGCTCACTC CGTGCTCCTG AACGATGTAT ACTACGCCTC TGAAATTGAG
    GAGGTGTGCT TGGTGGACGA TAACCAGTTC ACCCTGTCTA TAACTAACGA AAGTGGCCAG
    CTTAGCTTTA TTCACAACGA TTGCGACAAC ATTGTTCAAG CCATCATACA CATCCGAAAT
    CGATGGGAGC TTAGTCAGCC GGACTCCGTG ACGGTTCACC AAAAGATCCG ACCAAAAGAT
    GTGCCTGGAA CGCTGCTGAA CATGGCGCTA CTCAATCTGG GATCATGTGA CCCCAATCTA
    AGGACTGCTG CCTACAATTT GCTGTGTGCT TTGACCGCTA CTTTTGATCT AAAGATTGAG
    GGTCAACTGT TGGAGACCCA AGGACTTTGC ATACCCTCAA ATAATACCAT ATTTATCAAA
    TCCGTTAGCG AAAAGCTGGC CACCAATGAA CCGCATCTGA CTCTGGAATT CCTTGAGGAG
    TCCATTCAGG GCTTCCAGCG CAGCACCATA GAGCTGAAAC ATTTGTGTTT GGAGTACATG
    ACGCCATGGT TAAAGAACCT AGTCAAATTT TGCAAGTCCA ACGATGACTC CAAAAAGCTT
    AAGGTCTCCC AAATTCTGGA CAAGCTCATC AATTTAACTA TTGACCAAAA GGAAATGTAT
    CCCTCAGTGC AGGCCAAGAT CTGGGGATCA ATTGGACAGA TCCCCGAGCT GATCGACATG
    GTTTTGGATA ACTTTTTGCA CAAATCGATC ACATATGGCT TGGGATCACC ACAGGTGGAG
    ATTATGGCTG ACACGGCAGT GGCTCTCGCT TCAGCGAATG TTCAGCTGGT GTCCAAAAAG
    GTCATAACGC GAATATGCCG AGTCATGGAC AAATCCTGCA CAAATCCCAC ACAATATCTG
    GAGCAGCATA TGATGTGGGA CGATATTGCA ATTCTTGGTC GATACCTGCT CATGTTGTCG
    TTCAACAATT GTTTGGATGT GGCCACATCG GTGCCATATC TATTCCACAC CATTACGTTT
    TTGGTATGTT CAGGATCCCT GTCGATGCGA GCCTCTACTC ATGGCCTAGT GATCAACATC
    ATCCACTCGC TGTGCACATG CACAAATCCC TCATTTTCAG AGGAGGCGCA GCGAGTACTC
    CGACTGTCCC TGGATGAATT CTCACTGCCC AAGTTCTATC TACTCTTCGG CATCAGCAAG
    GTCAAGTCCG CAGCAGTTAC CGCTTTCCGC TCCAGCTGCC GTCACCCTAC AGATAAATGG
    CTAGGAAATG AAAGGGTTAC CCAGCCTCTG CCCGCCGATC GAGAGCGTTT ATCCCTACCA
    TCGCTTGAGG TTATCACGGA TGCCCTGCTG GAAATCATGG AGGCTTGCAT GCGAGATGTT
    CCGGACTGCG AATGGCTCAA CACCTGGACC TCTTTGGCTC GCAGTTTTGC TTTCTGTTAC
    AATCCAGCGC TACAGCCTAG AGCCCTTATA GTTTACGGGT GCATCAGCAA GAGTGTTACC
    GATCACGAGG TCAAGCAGTT ACTACGCATC CTGGTTAAGG CCCTCGAATC TTTCAACGAT
    CTCATTCTGA TCGAGGCTCT AGTAATGTGC TTAACTCGCA TTCAACCACT ACTCCGACCA
    GAGTCGCCCA TTCATCGGGC CCTCTTCTGG GTGGCCATTT CGGTGCTGCA GTTGGACGAG
    ATTACCCTGT ACGGCGCTGG ACTAGCTCTG CTTGAACAAA ATCTACACAC GCTTAAGTCA
    CAGGGCTGTT TTGACAAGAA GGAGACCATA GCCGAGGTCA TGATGAAGAC AAGGGAAAAG
    CTGGAGTGGC ATTTCAAGCA ACTGGATCAC GCCGTCGGCC TCTCCTTCCG CAGCAATTTC
    CACTTTGCCC TAGTGGGACA CTTGATTAAG GGCTTCCGCC ATCCTACACC TACAACCGTC
    TCACGCACCT CTCGTGTGCT GACGATGCTC TTGGGCATAA TAGCCAAACC CCTGCATCGG
    GACAAGTTCG AAGTAACACC TGACAGTGTG GCCTATTTGA CTGCGCTGGT GGCTGTCTCC
    GAGGAAGTCC GTTCCAGATG TCACGTAAAA CATGCCCTTC CCCGCTGGCC AGCCGATCTG
    AGTAGTAGTG TGGAAAATGG TGAAGCATCC GGCGGAGTGC AAGCTATCGG CCTGCCCCTG
    TCACGCCGTC AAAAAAGTTG GGACATCCTG GATCAGTCCG CCTTGCAGTT TGCCCGCCAA
    CACAAGGTTC CCACTCTTCA GAACGCGCGA GTTTTGTTCA AAACTCAACG CTCATTCTCG
    GTGCCGACGA CCAAAGATCC GAACAATGCC ACCGGCATCG AAGAACGTCA GGAACGCGGT
    TCGCGTTCGT CGGTGTCCAA CGAGTCCAAC GTACTGCTCG ATCCCGAGGT CTTACCTGAC
    CTGTCCATTC AAGCCCTGGT ACTGACTGTT TTGGCCACCT TGGTCAAGTA CTCTTCGGAT
    GAAGGCGAAA CGCGGGTTTT GTATCAGTAT TTGGCCGAAG GATCGGTGGT CTTTCCAAAG
    GTGTTTCCAG TTATCCATTC ACTGCTGGAC CAAAAGATAA ACAACATTTT GTCAGTGTCA
    CACGACCAAG TGGTGCTCAA CTCTGTGCAG AACATTATAC AAAATATGCT GGCCAGCGAA
    GATCCTTCCC AGCAGCAGCT GCACTTCCTG CAGAGTTGTG GATTTGGCGG ACTCTGGCGA
    TTTGCTGGTC CCTTCACCAA GTACAATATG ATGGGTGAGT CCTCGGAGCT GTTTGTCAAC
    TGTTTGGAGG CCATGGTTGA GACCTGTTTG CCAGGCGACG AATCCGCGCC GGTGCCACCA
    TCTCCGCGTC CATACAACCT GAGCTCCAGT CTGAGTAGCC TGACCCTGGG ATCACCCACG
    GATAAAGCAT TCTCATCGGA ATCATTAGAT TTTTATGACA ATTGCCCAGG CAGCGTCAGC
    AGCCTGCGAC GCGCCTCACA CAGCAAATCA CGCGCCAAAC ATCGAATTAA CGACAGTCCA
    TCACATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_733132.2
    CDS280..8688
    Translation

    Target ORF information:

    RefSeq Version NM_170253.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster neurofibromin 1, transcript variant B (Nf1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_170253.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGACCCAGA AGCCAGGCGA GTGGGCTAGC GCCCTTTTGG CACGTTTCGA GGATCAGCTG 
    CCAAACAGAA TCGGTGCCTA TGGCACGCAG GCCAGGATGA GCCAGGACCA ATTGGTGGCC
    TGTCTGATCC ACATATCGCG CTACCGCTTC TCCCTTGTCA TATCCGGTCT CACCAAGATG
    CTGCAGAGGG TCAATGAGGC GGCCCTTCAA AATCGCCATG AGCCAGAGCG TTGCTACTTT
    GAGTCGCTGG TCATCATACT GACCACTCTG GAACGCTGTC TGACCAACCA GACCAAGGAC
    ACAGCACGTT TTGAAGAGGC CATGAATGTG AAGCTTCTGC TGCGCGAGAT CTCCCAGTTT
    GTCGACGTCC AGAGCGACAG CAATCCGAAT GCCGCCCAGC TCAAGGCCCT GGCATCCAAG
    GTGCTCTTTG CCTTGTCCCA GAATCACTTC TCGGCGGTGT TCAATCGCAT ATCGGCCAGG
    ATTCAAGAGC TGACATCCTG TTCCGAGGAA AACCCGGACT ACAACGACAT AGAATTAATT
    CAACACATCG ATATGGATAT GATAAAGCTA ACCAAGCTGC TACAAGAAAC CATCACAAAG
    TTTCGATCGA AGCGTGCTCC ACCTTTGATC TTGCTTTACT CGCTGGAGAA GGCTATTTGG
    AACTGGATCG AGTACCATCC ACAAGAGTTC CAGGACTTGC AACGAGGAAC CAATCGAGAT
    ATATCAACCT GCTGGGAACC CCTGATGGAC TTTGTAGAGT ACTTTAAAAC CGAAAACAAG
    AAAAGCAAGA CTCTTGTTTG GCCGCTTCAA ATGCTCTTGC TGATATTGAA TCCCTCTTGC
    CTTGAGGCTG TTGTTAACGA ACTTCAGCAG TCGGAGAAAG AGAAGGAAAA GGACAAGGAA
    AAGGTTGCCT CGAAGTCAGC GCAATCCACG TCCCGGGACA AGGACTTCTC GGCCAAGCAG
    TTCATCGAGA GTATCAAGAG AGGCTTGGGC CAGCACTCAC CATCCAAACA GGTGACCGAA
    TCTGCGGCAA TTGCCTGTGT AAAGCTGTGC AAAGCATCCA CCTACATCAA CAACACCGAC
    TCCAATAATG TGGTCTTCAA GTTGGTGCAA TTCTTTATCA ACGATCTCAA GGCACTGCTC
    TTTAATCCAG CAAAGCCCTT TTCGCGCGGT CAGGGGTACA ACTTCGCTGA TATCGAGCTG
    ATGATTGACT GCTGGGTGTC TTGTTTCCGG ATTAATCCTC ACAACATCGA GGCTCTAAAG
    GTTTGCCTGA ATTTGTCCTC GCCGCAGGCT TACCATTTTG TAATTGTGTG CTCTCTGCTA
    AGGTTGGCTC ACATATACGT TGACTTCCGT TTGCAGAACA AGAACCCCTT TCGAATAGTA
    AACCAACCGA GATTGTCTTG GTGGCCGCAA ACGGATGTGG TTCACTACCG TTCTGCTGAG
    CTACGAGCTT TGTTTACGGA TACGCTCAAC AAGGCCACCC AGGGTTATAT AGCCCATACG
    CCACTGCGCT ACATAACCTC CCTGACGCTC AAATCTAAAG ATACCCAGAA AGGTTTAACC
    CGCGCTGAAG AGGGTCCCGC CCACAAAATG CTATTACTGC TACTCGTACG GCTAATTCAT
    GCAGATCCCA CGCTTTTGTT GAATACCCAA GGAAAAGTGG CACATGAAGT GCAGAGCTCC
    ACATTGGAGC TCATCAACGG CTTAGTTAGT TTGGTGCATC AAACTACCAT GCCCGATGTG
    GCACAAGAGG CTATGGAGGC GCTGCTTGCT CTGCACGCCC CGGAGAAAAT AGAGGTTTGG
    AATCCAGAGG CTCCGATCAA TACGTTCTGG GACGTTAGCT CCCAAGTACT GTTTTCAATC
    TCGCAAAAGC TCATTCAACA CCAAATTGCC AATTACACTG ATGTCTTAAA GTGGCTACGC
    GAGATCCTCA TCTGTCGGAA TACTTTTCTT CAGCGACACA AGGATTATGC ACATGTGGGA
    AGTCAGATTG CCATTTGCAA GCAGGCTCAC ATTAAGATGG AAGTTGTTTT CTTCATGTAC
    CTGTGGAGTG TTGATTTAGA TGCGGTGCTG ACATCGCTAT CGTGTTTTGG ACTACTATGC
    GAGGAGGCTG AGATTTGCTG CAGTTCTGAT GAATTGACAG TCGGATTTAT TATGCCGAAT
    TACCACATTT ACCAGGAGCT GGCTCAGTTG TCCACATCTG CAACGGACTC TCGCATTTGC
    TTTTTTGACA ACACGCACGG CAATGTGCTG AGCCGTCTTA CACTTCAAAA GCGCATAATG
    ACCCTATTGC GCAAGATCGA GCACTGTGTT CATGGTGTTC AGCCCGCCTG GGAGGAAACC
    TTCCGCAACT GGGAAGTATC CAGCAAGGTT TTGCAAACGT ACCCCAAATG TAAAGGAGAA
    GATGGCCAAG CGGAGGTTTT CCATCGTGGC ATGGGCAAGC GGCGAGCGAG TCACCAAAGC
    TCAGAGCACG ATTTGGAGGA ACAAATTAAT GAATGGGCCA ATATGACATG GTTTCTATTA
    GCCTTGGGTG GCGTCTGCCT TCATAAACGT AGCAGCAGTC GTCAAATGCT GCTGCAGCAA
    TCACAGAACA ATGCTTCTTT GGGATCACTT GCTCAAAACT CTCTTTACTC AAGCTCCACA
    AGTTCTGGAC ATGGCTCTCT GCATCCTAGC ACGGTTTCAC TATCCACTCT TCCCCCAGCA
    CCGCCACAAG ATGTCAGCTA TTGTCCTGTA ACACAATTCG TGGGCCAACT ATTGCGCCTG
    TTAGTTTGCA GCAACGAAAA AATTGGACTT AATATTCAGA AAAATGTGAA GGAACTGGTG
    GGCGAGGAGA TGTCCACCCA ACTGTATCCT ATACTCTTCG ACCAGGTTAG AGCCATCGTG
    GAGAAGTTCT TCGATCAGCA AGGTCAGGTG AACGTCAATG TGACCGACAT CAACACGCAG
    TTTATCGAGC ACACCATCTA CATAATGAAA TCAATTCTGG ATCCCAAAGC CAACAAGGAT
    CCCAACAACG ATCAACCCTC GCCATCGGAA CATTTAGGTG TTACAAGCAT CGAAGGCATG
    ATGCTAGGAA TAGTGCGCTA CGTTCGCCAC CTGGATATGA CTGTTTATGC CATTCGAATC
    AAGACAAAAT TGTGCCAGCT TGTGGAAGTA ATGATGAAGC GACGCGACGA CCTCGCCTTT
    CGCCAGGAGA TGAAGTTCCG GAATAAACTG GTTGAATACT TGACCGACTG GGTGATGGGC
    ACCTCCCATC AGATTGCACC GCCCAGCTCG GCGGATGCCG CTATTCTTAC CAACACATCC
    CTCATATTTC GCGATCTAGA CCAAGCCTGC ATGGAGGCAG TGGCTGCCTT GCTTCGGGGC
    CTTCCCCTTC AGCCTGAGGA ATCGGATCGA GGCGATTTGA TGGATGCAAA GAGTGCGCTC
    TTTTTAAAGT ACTTTACCCT ATTTATGAAC CTGCTGAATG ATTGCATCGA TAGCTCCGAG
    GCGGAGAAGG AAATGAATAA TACCCCACTA TTGCCTCCGC GCCCTCGAAT GGCAGCTGGA
    AAACTGACCG CTCTGCGAAA TGCCACTATT CTAGCCATGT CTAATTTGTT GGGTGCCAAC
    ATTGACTCTG GTTTGATGCA CTCCATCGAT CTGGGTTATA ATCCTGATTT GCAAACGCGT
    GCCGCTTTCA TGGAGGTGTT GACTCAAATC CTGCAACAAG GCACCGAATT CGATACCTTG
    GCCGAAACTG TTTTAGCTGA TCGATTTGAG CAACTGGTCC AACTTGTTAC AATGATCAGC
    GACAAAGGAG AACTTCCCAT AGCAATGGCT TTGGCTAATG TGGTGACTAC ATCCCAAATG
    GACGAGCTGG CTAGGGTTCT GGTCACCCTA TTCGACGCCA AACACCTGTT ATCGCCACTC
    CTATGGAATA TGTTTTATCG CGAGGTGGAG GTCTCAGACT GCATGCAGAC ACTTTTCCGT
    GGAAATTCTT TGGGCAGCAA AATCATGGCC TTTTGCTTTA AAATATACGG CGCGAGCTAT
    CTCCAAATGC TACTAGAGCC TTTAATTCGT CCGCTTCTGG ATGAGGAGGA GGAGACCTGT
    TTTGAGGTGG ATCCAGCTCG TCTGGATCCA ACAGAAGATA TTGAACAGCA CAGGAACAAC
    TTGATTGCCC TAACGCAGAA GGTGTTTGAC GCTATTATCA ACTCGTCGGA TCGCTTTCCC
    CCGCAGCTGC GATCCATGTG CCATTGCCTG TACCAAGTGC TAAGCAAACG CTTCCCGAAT
    CTTCTTCAAA ACAATATCGG TGCTGTAGGC ACAGTCATCT TCTTGCGGTT CATCAATCCC
    GCCATAGTTT CCCCACAGGA ATTGGGAATC GTTGACAAAC AAGTGCACAG CTCGGCCAAA
    CGAGGTCTAA TGCTAATGTC CAAGATCCTT CAAAACATTG CTAACCACGT GGAGTTCTCC
    AAGGAGCAGC ACATGTTGTG TTTCAACGAT TTTCTGCGCG ATCACTTTGA AGCTGGGCGG
    CGATTCTTCA TACAGATTGC ATCAGACTGT GAGACCGTGG ATCAGACCTC GCACAGCATG
    AGTTTTATTT CAGATGCGAA CGTACTGGCA CTGCATCGGT TGCTGTGGAC GCATCAGGAG
    AAGATCGGCG ACTATCTGTC TAGCAGTCGA GACCACAAGG CGGTTGGAAG GCGGCCCTTC
    GACAAGATGG CTACTTTGCT AGCATACCTG GGACCACCGG AGCACAAGCC CGTGGATTCG
    CACATGATGT TCAGCTCGTA TGCACGCTGG AGCTCCATTG ACATGTCGTC GACAAACTTC
    GAGGAGATCA TGGTCAAGCA CCAAATGCAC GAGAAGGAGG AATTCAAGAC CCTCAAGTCG
    ATGAACATAT TCTACCAGGC CGGAACAAGC AAATCTGGCT ATCCTGTCTT TTACTATATA
    GCAAGACGAT ACAAGATTGG AGAAACGAAT GGAGACTTAC TTATATATCA CGTCATACTC
    ACTCTGAAAC CATTCTGCCA CTCGCCCTTC GAGGTGGTCA TCGATTTTAC GCACACCTGT
    TCAGATAATC GGTTCCGCAC CGAGTTTCTG CAGAAGTGGT TTTATGTCCT GCCCACGGTA
    GCCTACGAAA ATGTCCATGC TGTGTACATC TATAACTGTA ACTCGTGGGT GCGCGAATAT
    ACCAAGTTCC ACGATCGCAT TCTGGCACCA TTAAAGGGAA ATCGCAAACT TCTGTTCCTG
    GAGTCACCCA ACAAACTTAC TGATTTCATC GACGCGGAGC AACAGAAATT GCCTGGAGCA
    ACTCTTTCCT TGGACGAAGA TTTAAAGGTA TTCAGCAACG CGCTGAAGCT CAGCCATAAA
    GACACAAAGG TGGCTATCAA AGTGGGTCCC ACCGCATTGC AAATTACATC TGCCGAAAAG
    ACAAAGGTCT TGGCTCACTC CGTGCTCCTG AACGATGTAT ACTACGCCTC TGAAATTGAG
    GAGGTGTGCT TGGTGGACGA TAACCAGTTC ACCCTGTCTA TAACTAACGA AAGTGGCCAG
    CTTAGCTTTA TTCACAACGA TTGCGACAAC ATTGTTCAAG CCATCATACA CATCCGAAAT
    CGATGGGAGC TTAGTCAGCC GGACTCCGTG ACGGTTCACC AAAAGATCCG ACCAAAAGAT
    GTGCCTGGAA CGCTGCTGAA CATGGCGCTA CTCAATCTGG GATCATGTGA CCCCAATCTA
    AGGACTGCTG CCTACAATTT GCTGTGTGCT TTGACCGCTA CTTTTGATCT AAAGATTGAG
    GGTCAACTGT TGGAGACCCA AGGACTTTGC ATACCCTCAA ATAATACCAT ATTTATCAAA
    TCCGTTAGCG AAAAGCTGGC CACCAATGAA CCGCATCTGA CTCTGGAATT CCTTGAGGAG
    TCCATTCAGG GCTTCCAGCG CAGCACCATA GAGCTGAAAC ATTTGTGTTT GGAGTACATG
    ACGCCATGGT TAAAGAACCT AGTCAAATTT TGCAAGTCCA ACGATGACTC CAAAAAGCTT
    AAGGTCTCCC AAATTCTGGA CAAGCTCATC AATTTAACTA TTGACCAAAA GGAAATGTAT
    CCCTCAGTGC AGGCCAAGAT CTGGGGATCA ATTGGACAGA TCCCCGAGCT GATCGACATG
    GTTTTGGATA ACTTTTTGCA CAAATCGATC ACATATGGCT TGGGATCACC ACAGGTGGAG
    ATTATGGCTG ACACGGCAGT GGCTCTCGCT TCAGCGAATG TTCAGCTGGT GTCCAAAAAG
    GTCATAACGC GAATATGCCG AGTCATGGAC AAATCCTGCA CAAATCCCAC ACAATATCTG
    GAGCAGCATA TGATGTGGGA CGATATTGCA ATTCTTGGTC GATACCTGCT CATGTTGTCG
    TTCAACAATT GTTTGGATGT GGCCACATCG GTGCCATATC TATTCCACAC CATTACGTTT
    TTGGTATGTT CAGGATCCCT GTCGATGCGA GCCTCTACTC ATGGCCTAGT GATCAACATC
    ATCCACTCGC TGTGCACATG CACAAATCCC TCATTTTCAG AGGAGGCGCA GCGAGTACTC
    CGACTGTCCC TGGATGAATT CTCACTGCCC AAGTTCTATC TACTCTTCGG CATCAGCAAG
    GTCAAGTCCG CAGCAGTTAC CGCTTTCCGC TCCAGCTGCC GTCACCCTAC AGATAAATGG
    CTAGGAAATG AAAGGGTTAC CCAGCCTCTG CCCGCCGATC GAGAGCGTTT ATCCCTACCA
    TCGCTTGAGG TTATCACGGA TGCCCTGCTG GAAATCATGG AGGCTTGCAT GCGAGATGTT
    CCGGACTGCG AATGGCTCAA CACCTGGACC TCTTTGGCTC GCAGTTTTGC TTTCTGTTAC
    AATCCAGCGC TACAGCCTAG AGCCCTTATA GTTTACGGGT GCATCAGCAA GAGTGTTACC
    GATCACGAGG TCAAGCAGTT ACTACGCATC CTGGTTAAGG CCCTCGAATC TTTCAACGAT
    CTCATTCTGA TCGAGGCTCT AGTAATGTGC TTAACTCGCA TTCAACCACT ACTCCGACCA
    GAGTCGCCCA TTCATCGGGC CCTCTTCTGG GTGGCCATTT CGGTGCTGCA GTTGGACGAG
    ATTACCCTGT ACGGCGCTGG ACTAGCTCTG CTTGAACAAA ATCTACACAC GCTTAAGTCA
    CAGGGCTGTT TTGACAAGAA GGAGACCATA GCCGAGGTCA TGATGAAGAC AAGGGAAAAG
    CTGGAGTGGC ATTTCAAGCA ACTGGATCAC GCCGTCGGCC TCTCCTTCCG CAGCAATTTC
    CACTTTGCCC TAGTGGGACA CTTGATTAAG GGCTTCCGCC ATCCTACACC TACAACCGTC
    TCACGCACCT CTCGTGTGCT GACGATGCTC TTGGGCATAA TAGCCAAACC CCTGCATCGG
    GACAAGTTCG AAGTAACACC TGACAGTGTG GCCTATTTGA CTGCGCTGGT GGCTGTCTCC
    GAGGAAGTCC GTTCCAGATG TCACGTAAAA CATGCCCTTC CCCGCTGGCC AGCCGATCTG
    AGTAGTAGTG TGGAAAATGG TGAAGCATCC GGCGGAGTGC AAGCTATCGG CCTGCCCCTG
    TCACGCCGTC AAAAAAGTTG GGACATCCTG GATCAGTCCG CCTTGCAGTT TGCCCGCCAA
    CACAAGGTTC CCACTCTTCA GAACGCGCGA GTTTTGTTCA AAACTCAACG CTCATTCTCG
    GTGCCGACGA CCAAAGATCC GAACAATGCC ACCGGCATCG AAGAACGTCA GGAACGCGGT
    TCGCGTTCGT CGGTGTCCAA CGAGTCCAAC GTACTGCTCG ATCCCGAGGT CTTACCTGAC
    CTGTCCATTC AAGCCCTGGT ACTGACTGTT TTGGCCACCT TGGTCAAGTA CTCTTCGGAT
    GAAGGCGAAA CGCGGGTTTT GTATCAGTAT TTGGCCGAAG GATCGGTGGT CTTTCCAAAG
    GTGTTTCCAG TTATCCATTC ACTGCTGGAC CAAAAGATAA ACAACATTTT GTCAGTGTCA
    CACGACCAAG TGGTGCTCAA CTCTGTGCAG AACATTATAC AAAATATGCT GGCCAGCGAA
    GATCCTTCCC AGCAGCAGCT GCACTTCCTG CAGAGTTGTG GATTTGGCGG ACTCTGGCGA
    TTTGCTGGTC CCTTCACCAA GTACAATATG ATGGGTGAGT CCTCGGAGCT GTTTGTCAAC
    TGTTTGGAGG CCATGGTTGA GACCTGTTTG CCAGGCGACG AATCCGCGCC GGTGCCACCA
    TCTCCGCGTC CATACAACCT GAGCTCCAGT CTGAGTAGCC TGACCCTGGG ATCACCCACG
    GATAAAGCAT TCTCATCGGA ATCATTAGAT TTTTATGACA ATTGCCCAGG CAGCGTCAGC
    AGCCTGCGAC GCGCCTCACA CAGCAAATCA CGCGCCAAAC ATCGAATTAA CGACAGTCCA
    TCACATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08849
    Clone ID Related Accession (Same CDS sequence) NM_001276049.1
    Accession Version NM_001276049.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8382bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product neurofibromin 1, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGACCCAGA AGCCAGGCGA GTGGGCTAGC GCCCTTTTGG CACGTTTCGA GGATCAGCTG 
    CCAAACAGAA TCGGTGCCTA TGGCACGCAG GCCAGGATGA GCCAGGACCA ATTGGTGGCC
    TGTCTGATCC ACATATCGCG CTACCGCTTC TCCCTTGTCA TATCCGGTCT CACCAAGATG
    CTGCAGAGGG TCAATGAGGC GGCCCTTCAA AATCGCCATG AGCCAGAGCG TTGCTACTTT
    GAGTCGCTGG TCATCATACT GACCACTCTG GAACGCTGTC TGACCAACCA GACCAAGGAC
    ACAGCACGTT TTGAAGAGGC CATGAATGTG AAGCTTCTGC TGCGCGAGAT CTCCCAGTTT
    GTCGACGTCC AGAGCGACAG CAATCCGAAT GCCGCCCAGC TCAAGGCCCT GGCATCCAAG
    GTGCTCTTTG CCTTGTCCCA GAATCACTTC TCGGCGGTGT TCAATCGCAT ATCGGCCAGG
    ATTCAAGAGC TGACATCCTG TTCCGAGGAA AACCCGGACT ACAACGACAT AGAATTAATT
    CAACACATCG ATATGGATAT GATAAAGCTA ACCAAGCTGC TACAAGAAAC CATCACAAAG
    TTTCGATCGA AGCGTGCTCC ACCTTTGATC TTGCTTTACT CGCTGGAGAA GGCTATTTGG
    AACTGGATCG AGTACCATCC ACAAGAGTTC CAGGACTTGC AACGAGGAAC CAATCGAGAT
    ATATCAACCT GCTGGGAACC CCTGATGGAC TTTGTAGAGT ACTTTAAAAC CGAAAACAAG
    AAAAGCAAGA CTCTTGTTTG GCCGCTTCAA ATGCTCTTGC TGATATTGAA TCCCTCTTGC
    CTTGAGGCTG TTGTTAACGA ACTTCAGCAG TCGGAGAAAG AGAAGGAAAA GGACAAGGAA
    AAGGTTGCCT CGAAGTCAGC GCAATCCACG TCCCGGGACA AGGACTTCTC GGCCAAGCAG
    TTCATCGAGA GTATCAAGAG AGGCTTGGGC CAGCACTCAC CATCCAAACA GGTGACCGAA
    TCTGCGGCAA TTGCCTGTGT AAAGCTGTGC AAAGCATCCA CCTACATCAA CAACACCGAC
    TCCAATAATG TGGTCTTCAA GTTGGTGCAA TTCTTTATCA ACGATCTCAA GGCACTGCTC
    TTTAATCCAG CAAAGCCCTT TTCGCGCGGT CAGGGGTACA ACTTCGCTGA TATCGAGCTG
    ATGATTGACT GCTGGGTGTC TTGTTTCCGG ATTAATCCTC ACAACATCGA GGCTCTAAAG
    GTTTGCCTGA ATTTGTCCTC GCCGCAGGCT TACCATTTTG TAATTGTGTG CTCTCTGCTA
    AGGTTGGCTC ACATATACGT TGACTTCCGT TTGCAGAACA AGAACCCCTT TCGAATAGTA
    AACCAACCGA GATTGTCTTG GTGGCCGCAA ACGGATGTGG TTCACTACCG TTCTGCTGAG
    CTACGAGCTT TGTTTACGGA TACGCTCAAC AAGGCCACCC AGGGTTATAT AGCCCATACG
    CCACTGCGCT ACATAACCTC CCTGACGCTC AAATCTAAAG ATACCCAGAA AGGTTTAACC
    CGCGCTGAAG AGGGTCCCGC CCACAAAATG CTATTACTGC TACTCGTACG GCTAATTCAT
    GCAGATCCCA CGCTTTTGTT GAATACCCAA GGAAAAGTGG CACATGAAGT GCAGAGCTCC
    ACATTGGAGC TCATCAACGG CTTAGTTAGT TTGGTGCATC AAACTACCAT GCCCGATGTG
    GCACAAGAGG CTATGGAGGC GCTGCTTGCT CTGCACGCCC CGGAGAAAAT AGAGGTTTGG
    AATCCAGAGG CTCCGATCAA TACGTTCTGG GACGTTAGCT CCCAAGTACT GTTTTCAATC
    TCGCAAAAGC TCATTCAACA CCAAATTGCC AATTACACTG ATGTCTTAAA GTGGCTACGC
    GAGATCCTCA TCTGTCGGAA TACTTTTCTT CAGCGACACA AGGATTATGC ACATGTGGGA
    AGTCAGATTG CCATTTGCAA GCAGGCTCAC ATTAAGATGG AAGTTGTTTT CTTCATGTAC
    CTGTGGAGTG TTGATTTAGA TGCGGTGCTG ACATCGCTAT CGTGTTTTGG ACTACTATGC
    GAGGAGGCTG AGATTTGCTG CAGTTCTGAT GAATTGACAG TCGGATTTAT TATGCCGAAT
    TACCACATTT ACCAGGAGCT GGCTCAGTTG TCCACATCTG CAACGGACTC TCGCATTTGC
    TTTTTTGACA ACACGCACGG CAATGTGCTG AGCCGTCTTA CACTTCAAAA GCGCATAATG
    ACCCTATTGC GCAAGATCGA GCACTGTGTT CATGGTGTTC AGCCCGCCTG GGAGGAAACC
    TTCCGCAACT GGGAAGTATC CAGCAAGGTT TTGCAAACGT ACCCCAAATG TAAAGGAGAA
    GATGGCCAAG CGGAGGTTTT CCATCGTGGC ATGGGCAAGC GGCGAGCGAG TCACCAAAGC
    TCAGAGCACG ATTTGGAGGA ACAAATTAAT GAATGGGCCA ATATGACATG GTTTCTATTA
    GCCTTGGGTG GCGTCTGCCT TCATAAACGT AGCAGCAGTC GTCAAATGCT GCTGCAGCAA
    TCACAGAACA ATGCTTCTTT GGGATCACTT GCTCAAAACT CTCTTTACTC AAGCTCCACA
    AGTTCTGGAC ATGGCTCTCT GCATCCTAGC ACGGTTTCAC TATCCACTCT TCCCCCAGCA
    CCGCCACAAG ATGTCAGCTA TTGTCCTGTA ACACAATTCG TGGGCCAACT ATTGCGCCTG
    TTAGTTTGCA GCAACGAAAA AATTGGACTT AATATTCAGA AAAATGTGAA GGAACTGGTG
    GGCGAGGAGA TGTCCACCCA ACTGTATCCT ATACTCTTCG ACCAGGTTAG AGCCATCGTG
    GAGAAGTTCT TCGATCAGCA AGGTCAGGTG AACGTCAATG TGACCGACAT CAACACGCAG
    TTTATCGAGC ACACCATCTA CATAATGAAA TCAATTCTGG ATCCCAAAGC CAACAAGGAT
    CCCAACAACG ATCAACCCTC GCCATCGGAA CATTTAGGTG TTACAAGCAT CGAAGGCATG
    ATGCTAGGAA TAGTGCGCTA CGTTCGCCAC CTGGATATGA CTGTTTATGC CATTCGAATC
    AAGACAAAAT TGTGCCAGCT TGTGGAAGTA ATGATGAAGC GACGCGACGA CCTCGCCTTT
    CGCCAGGAGA TGAAGTTCCG GAATAAACTG GTTGAATACT TGACCGACTG GGTGATGGGC
    ACCTCCCATC AGATTGCACC GCCCAGCTCG GCGGATGCCG CTATTCTTAC CAACACATCC
    CTCATATTTC GCGATCTAGA CCAAGCCTGC ATGGAGGCAG TGGCTGCCTT GCTTCGGGGC
    CTTCCCCTTC AGCCTGAGGA ATCGGATCGA GGCGATTTGA TGGATGCAAA GAGTGCGCTC
    TTTTTAAAGT ACTTTACCCT ATTTATGAAC CTGCTGAATG ATTGCATCGA TAGCTCCGAG
    GCGGAGAAGG AAATGAATAA TACCCCACTA TTGCCTCCGC GCCCTCGAAT GGCAGCTGGA
    AAACTGACCG CTCTGCGAAA TGCCACTATT CTAGCCATGT CTAATTTGTT GGGTGCCAAC
    ATTGACTCTG GTTTGATGCA CTCCATCGAT CTGGGTTATA ATCCTGATTT GCAAACGCGT
    GCCGCTTTCA TGGAGGTGTT GACTCAAATC CTGCAACAAG GCACCGAATT CGATACCTTG
    GCCGAAACTG TTTTAGCTGA TCGATTTGAG CAACTGGTCC AACTTGTTAC AATGATCAGC
    GACAAAGGAG AACTTCCCAT AGCAATGGCT TTGGCTAATG TGGTGACTAC ATCCCAAATG
    GACGAGCTGG CTAGGGTTCT GGTCACCCTA TTCGACGCCA AACACCTGTT ATCGCCACTC
    CTATGGAATA TGTTTTATCG CGAGGTGGAG GTCTCAGACT GCATGCAGAC ACTTTTCCGT
    GGAAATTCTT TGGGCAGCAA AATCATGGCC TTTTGCTTTA AAATATACGG CGCGAGCTAT
    CTCCAAATGC TACTAGAGCC TTTAATTCGT CCGCTTCTGG ATGAGGAGGA GGAGACCTGT
    TTTGAGGTGG ATCCAGCTCG TCTGGATCCA ACAGAAGATA TTGAACAGCA CAGGAACAAC
    TTGATTGCCC TAACGCAGAA GGTGTTTGAC GCTATTATCA ACTCGTCGGA TCGCTTTCCC
    CCGCAGCTGC GATCCATGTG CCATTGCCTG TACCAAGTGC TAAGCAAACG CTTCCCGAAT
    CTTCTTCAAA ACAATATCGG TGCTGTAGGC ACAGTCATCT TCTTGCGGTT CATCAATCCC
    GCCATAGTTT CCCCACAGGA ATTGGGAATC GTTGACAAAC AAGTGCACAG CTCGGCCAAA
    CGAGGTCTAA TGCTAATGTC CAAGATCCTT CAAAACATTG CTAACCACGT GGAGTTCTCC
    AAGGAGCAGC ACATGTTGTG TTTCAACGAT TTTCTGCGCG ATCACTTTGA AGCTGGGCGG
    CGATTCTTCA TACAGATTGC ATCAGACTGT GAGACCGTGG ATCAGACCTC GCACAGCATG
    AGTTTTATTT CAGATGCGAA CGTACTGGCA CTGCATCGGT TGCTGTGGAC GCATCAGGAG
    AAGATCGGCG ACTATCTGTC TAGCAGTCGA GACCACAAGG CGGTTGGAAG GCGGCCCTTC
    GACAAGATGG CTACTTTGCT AGCATACCTG GGACCACCGG AGCACAAGCC CGTGGATTCG
    CACATGATGT TCAGCTCGTA TGCACGCTGG AGCTCCATTG ACATGTCGTC GACAAACTTC
    GAGGAGATCA TGGTCAAGCA CCAAATGCAC GAGAAGGAGG AATTCAAGAC CCTCAAGTCG
    ATGAACATAT TCTACCAGGC CGGAACAAGC AAATCTGGCT ATCCTGTCTT TTACTATATA
    GCAAGACGAT ACAAGATTGG AGAAACGAAT GGAGACTTAC TTATATATCA CGTCATACTC
    ACTCTGAAAC CATTCTGCCA CTCGCCCTTC GAGGTGGTCA TCGATTTTAC GCACACCTGT
    TCAGATAATC GGTTCCGCAC CGAGTTTCTG CAGAAGTGGT TTTATGTCCT GCCCACGGTA
    GCCTACGAAA ATGTCCATGC TGTGTACATC TATAACTGTA ACTCGTGGGT GCGCGAATAT
    ACCAAGTTCC ACGATCGCAT TCTGGCACCA TTAAAGGGAA ATCGCAAACT TCTGTTCCTG
    GAGTCACCCA ACAAACTTAC TGATTTCATC GACGCGGAGC AACAGAAATT GCCTGGAGCA
    ACTCTTTCCT TGGACGAAGA TTTAAAGGTA TTCAGCAACG CGCTGAAGCT CAGCCATAAA
    GACACAAAGG TGGCTATCAA AGTGGGTCCC ACCGCATTGC AAATTACATC TGCCGAAAAG
    ACAAAGGTCT TGGCTCACTC CGTGCTCCTG AACGATGTAT ACTACGCCTC TGAAATTGAG
    GAGGTGTGCT TGGTGGACGA TAACCAGTTC ACCCTGTCTA TAACTAACGA AAGTGGCCAG
    CTTAGCTTTA TTCACAACGA TTGCGACAAC ATTGTTCAAG CCATCATACA CATCCGAAAT
    CGATGGGAGC TTAGTCAGCC GGACTCCGTG ACGGTTCACC AAAAGATCCG ACCAAAAGAT
    GTGCCTGGAA CGCTGCTGAA CATGGCGCTA CTCAATCTGG GATCATGTGA CCCCAATCTA
    AGGACTGCTG CCTACAATTT GCTGTGTGCT TTGACCGCTA CTTTTGATCT AAAGATTGAG
    GGTCAACTGT TGGAGACCCA AGGACTTTGC ATACCCTCAA ATAATACCAT ATTTATCAAA
    TCCGTTAGCG AAAAGCTGGC CACCAATGAA CCGCATCTGA CTCTGGAATT CCTTGAGGAG
    TCCATTCAGG GCTTCCAGCG CAGCACCATA GAGCTGAAAC ATTTGTGTTT GGAGTACATG
    ACGCCATGGT TAAAGAACCT AGTCAAATTT TGCAAGTCCA ACGATGACTC CAAAAAGCTT
    AAGGTCTCCC AAATTCTGGA CAAGCTCATC AATTTAACTA TTGACCAAAA GGAAATGTAT
    CCCTCAGTGC AGGCCAAGAT CTGGGGATCA ATTGGACAGA TCCCCGAGCT GATCGACATG
    GTTTTGGATA ACTTTTTGCA CAAATCGATC ACATATGGCT TGGGATCACC ACAGGTGGAG
    ATTATGGCTG ACACGGCAGT GGCTCTCGCT TCAGCGAATG TTCAGCTGGT GTCCAAAAAG
    GTCATAACGC GAATATGCCG AGTCATGGAC AAATCCTGCA CAAATCCCAC ACAATATCTG
    GAGCAGCATA TGATGTGGGA CGATATTGCA ATTCTTGGTC GATACCTGCT CATGTTGTCG
    TTCAACAATT GTTTGGATGT GGCCACATCG GTGCCATATC TATTCCACAC CATTACGTTT
    TTGGTATGTT CAGGATCCCT GTCGATGCGA GCCTCTACTC ATGGCCTAGT GATCAACATC
    ATCCACTCGC TGTGCACATG CACAAATCCC TCATTTTCAG AGGAGGCGCA GCGAGTACTC
    CGACTGTCCC TGGATGAATT CTCACTGCCC AAGTTCTATC TACTCTTCGG CATCAGCAAG
    GTCAAGTCCG CAGCAGTTAC CGCTTTCCGC TCCAGCTGCC GTCACCCTAC AGATAAATGG
    CTAGGAAATG AAAGGGTTAC CCAGCCTCTG CCCGCCGATC GAGAGCGTTT ATCCCTACCA
    TCGCTTGAGG TTATCACGGA TGCCCTGCTG GAAATCATGG AGGCTTGCAT GCGAGATGTT
    CCGGACTGCG AATGGCTCAA CACCTGGACC TCTTTGGCTC GCAGTTTTGC TTTCTGTTAC
    AATCCAGCGC TACAGCCTAG AGCCCTTATA GTTTACGGGT GCATCAGCAA GAGTGTTACC
    GATCACGAGG TCAAGCAGTT ACTACGCATC CTGGTTAAGG CCCTCGAATC TTTCAACGAT
    CTCATTCTGA TCGAGGCTCT AGTAATGTGC TTAACTCGCA TTCAACCACT ACTCCGACCA
    GAGTCGCCCA TTCATCGGGC CCTCTTCTGG GTGGCCATTT CGGTGCTGCA GTTGGACGAG
    ATTACCCTGT ACGGCGCTGG ACTAGCTCTG CTTGAACAAA ATCTACACAC GCTTAAGTCA
    CAGGGCTGTT TTGACAAGAA GGAGACCATA GCCGAGGTCA TGATGAAGAC AAGGGAAAAG
    CTGGAGTGGC ATTTCAAGCA ACTGGATCAC GCCGTCGGCC TCTCCTTCCG CAGCAATTTC
    CACTTTGCCC TAGTGGGACA CTTGATTAAG GGCTTCCGCC ATCCTACACC TACAACCGTC
    TCACGCACCT CTCGTGTGCT GACGATGCTC TTGGGCATAA TAGCCAAACC CCTGCATCGG
    GACAAGTTCG AAGTAACACC TGACAGTGTG GCCTATTTGA CTGCGCTGGT GGCTGTCTCC
    GAGGAAGTCC GTTCCAGATG TCACGTAAAA CATGCCCTTC CCCGCTGGCC AGCCGATCTG
    AGTAGTAGTG TGGAAAATGG TGAAGCATCC GGCGGAGTGC AAGCTATCGG CCTGCCCCTG
    TCACGCCGTC AAAAAAGTTG GGACATCCTG GATCAGTCCG CCTTGCAGTT TGCCCGCCAA
    CACAAGGTTC CCACTCTTCA GAACGCGCGA GTTTTGTTCA AAACTCAACG CTCATTCTCG
    GTGCCGACGA CCAAAGATCC GAACAATGCC ACCGGCATCG AAGAACGTCA GGAACGCGGT
    TCGCGTTCGT CGGTGTCCAA CGAGTCCAAC GTACTGCTCG ATCCCGAGGT CTTACCTGAC
    CTGTCCATTC AAGCCCTGGT ACTGACTGTT TTGGCCACCT TGGTCAAGTA CTCTTCGGAT
    GAAGGCGAAA CGCGGGTTTT GTATCAGTAT TTGGCCGAAG GATCGGTGGT CTTTCCAAAG
    GTGTTTCCAG TTATCCATTC ACTGCTGGAC CAAAAGATAA ACAACATTTT GTCAGTGTCA
    CACGACCAAG TGGTGCTCAA CTCTGTGCAG AACATTATAC AAAATATGCT GGCCAGCGAA
    GATCCTTCCC AGCAGCAGCT GCACTTCCTG CAGAGTTGTG GATTTGGCGG ACTCTGGCGA
    TTTGCTGGTC CCTTCACCAA GTACAATATG ATGGGTGAGT CCTCGGAGCT GTTTGTCAAC
    TGTTTGGAGG CCATGGTTGA GACCTGTTTG CCAGGCGACG AATCCGCGCC GGTGCCACCA
    TCTCCGCGTC CATACAACCT GAGCTCCAGT CTGAGTAGCC TGACCCTGGG ATCACCCACG
    GATAAAGCTG CATGAATAAC AACTACAAAC CCGCGCACTA GACGTACCAC ACTTATCGCA
    AAAGTAATGC GTTTCCTAAA TCTGCTTAAA AAGTGCTTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001262978.1
    CDS280..8661
    Translation

    Target ORF information:

    RefSeq Version NM_001276049.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster neurofibromin 1, transcript variant E (Nf1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001276049.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGACCCAGA AGCCAGGCGA GTGGGCTAGC GCCCTTTTGG CACGTTTCGA GGATCAGCTG 
    CCAAACAGAA TCGGTGCCTA TGGCACGCAG GCCAGGATGA GCCAGGACCA ATTGGTGGCC
    TGTCTGATCC ACATATCGCG CTACCGCTTC TCCCTTGTCA TATCCGGTCT CACCAAGATG
    CTGCAGAGGG TCAATGAGGC GGCCCTTCAA AATCGCCATG AGCCAGAGCG TTGCTACTTT
    GAGTCGCTGG TCATCATACT GACCACTCTG GAACGCTGTC TGACCAACCA GACCAAGGAC
    ACAGCACGTT TTGAAGAGGC CATGAATGTG AAGCTTCTGC TGCGCGAGAT CTCCCAGTTT
    GTCGACGTCC AGAGCGACAG CAATCCGAAT GCCGCCCAGC TCAAGGCCCT GGCATCCAAG
    GTGCTCTTTG CCTTGTCCCA GAATCACTTC TCGGCGGTGT TCAATCGCAT ATCGGCCAGG
    ATTCAAGAGC TGACATCCTG TTCCGAGGAA AACCCGGACT ACAACGACAT AGAATTAATT
    CAACACATCG ATATGGATAT GATAAAGCTA ACCAAGCTGC TACAAGAAAC CATCACAAAG
    TTTCGATCGA AGCGTGCTCC ACCTTTGATC TTGCTTTACT CGCTGGAGAA GGCTATTTGG
    AACTGGATCG AGTACCATCC ACAAGAGTTC CAGGACTTGC AACGAGGAAC CAATCGAGAT
    ATATCAACCT GCTGGGAACC CCTGATGGAC TTTGTAGAGT ACTTTAAAAC CGAAAACAAG
    AAAAGCAAGA CTCTTGTTTG GCCGCTTCAA ATGCTCTTGC TGATATTGAA TCCCTCTTGC
    CTTGAGGCTG TTGTTAACGA ACTTCAGCAG TCGGAGAAAG AGAAGGAAAA GGACAAGGAA
    AAGGTTGCCT CGAAGTCAGC GCAATCCACG TCCCGGGACA AGGACTTCTC GGCCAAGCAG
    TTCATCGAGA GTATCAAGAG AGGCTTGGGC CAGCACTCAC CATCCAAACA GGTGACCGAA
    TCTGCGGCAA TTGCCTGTGT AAAGCTGTGC AAAGCATCCA CCTACATCAA CAACACCGAC
    TCCAATAATG TGGTCTTCAA GTTGGTGCAA TTCTTTATCA ACGATCTCAA GGCACTGCTC
    TTTAATCCAG CAAAGCCCTT TTCGCGCGGT CAGGGGTACA ACTTCGCTGA TATCGAGCTG
    ATGATTGACT GCTGGGTGTC TTGTTTCCGG ATTAATCCTC ACAACATCGA GGCTCTAAAG
    GTTTGCCTGA ATTTGTCCTC GCCGCAGGCT TACCATTTTG TAATTGTGTG CTCTCTGCTA
    AGGTTGGCTC ACATATACGT TGACTTCCGT TTGCAGAACA AGAACCCCTT TCGAATAGTA
    AACCAACCGA GATTGTCTTG GTGGCCGCAA ACGGATGTGG TTCACTACCG TTCTGCTGAG
    CTACGAGCTT TGTTTACGGA TACGCTCAAC AAGGCCACCC AGGGTTATAT AGCCCATACG
    CCACTGCGCT ACATAACCTC CCTGACGCTC AAATCTAAAG ATACCCAGAA AGGTTTAACC
    CGCGCTGAAG AGGGTCCCGC CCACAAAATG CTATTACTGC TACTCGTACG GCTAATTCAT
    GCAGATCCCA CGCTTTTGTT GAATACCCAA GGAAAAGTGG CACATGAAGT GCAGAGCTCC
    ACATTGGAGC TCATCAACGG CTTAGTTAGT TTGGTGCATC AAACTACCAT GCCCGATGTG
    GCACAAGAGG CTATGGAGGC GCTGCTTGCT CTGCACGCCC CGGAGAAAAT AGAGGTTTGG
    AATCCAGAGG CTCCGATCAA TACGTTCTGG GACGTTAGCT CCCAAGTACT GTTTTCAATC
    TCGCAAAAGC TCATTCAACA CCAAATTGCC AATTACACTG ATGTCTTAAA GTGGCTACGC
    GAGATCCTCA TCTGTCGGAA TACTTTTCTT CAGCGACACA AGGATTATGC ACATGTGGGA
    AGTCAGATTG CCATTTGCAA GCAGGCTCAC ATTAAGATGG AAGTTGTTTT CTTCATGTAC
    CTGTGGAGTG TTGATTTAGA TGCGGTGCTG ACATCGCTAT CGTGTTTTGG ACTACTATGC
    GAGGAGGCTG AGATTTGCTG CAGTTCTGAT GAATTGACAG TCGGATTTAT TATGCCGAAT
    TACCACATTT ACCAGGAGCT GGCTCAGTTG TCCACATCTG CAACGGACTC TCGCATTTGC
    TTTTTTGACA ACACGCACGG CAATGTGCTG AGCCGTCTTA CACTTCAAAA GCGCATAATG
    ACCCTATTGC GCAAGATCGA GCACTGTGTT CATGGTGTTC AGCCCGCCTG GGAGGAAACC
    TTCCGCAACT GGGAAGTATC CAGCAAGGTT TTGCAAACGT ACCCCAAATG TAAAGGAGAA
    GATGGCCAAG CGGAGGTTTT CCATCGTGGC ATGGGCAAGC GGCGAGCGAG TCACCAAAGC
    TCAGAGCACG ATTTGGAGGA ACAAATTAAT GAATGGGCCA ATATGACATG GTTTCTATTA
    GCCTTGGGTG GCGTCTGCCT TCATAAACGT AGCAGCAGTC GTCAAATGCT GCTGCAGCAA
    TCACAGAACA ATGCTTCTTT GGGATCACTT GCTCAAAACT CTCTTTACTC AAGCTCCACA
    AGTTCTGGAC ATGGCTCTCT GCATCCTAGC ACGGTTTCAC TATCCACTCT TCCCCCAGCA
    CCGCCACAAG ATGTCAGCTA TTGTCCTGTA ACACAATTCG TGGGCCAACT ATTGCGCCTG
    TTAGTTTGCA GCAACGAAAA AATTGGACTT AATATTCAGA AAAATGTGAA GGAACTGGTG
    GGCGAGGAGA TGTCCACCCA ACTGTATCCT ATACTCTTCG ACCAGGTTAG AGCCATCGTG
    GAGAAGTTCT TCGATCAGCA AGGTCAGGTG AACGTCAATG TGACCGACAT CAACACGCAG
    TTTATCGAGC ACACCATCTA CATAATGAAA TCAATTCTGG ATCCCAAAGC CAACAAGGAT
    CCCAACAACG ATCAACCCTC GCCATCGGAA CATTTAGGTG TTACAAGCAT CGAAGGCATG
    ATGCTAGGAA TAGTGCGCTA CGTTCGCCAC CTGGATATGA CTGTTTATGC CATTCGAATC
    AAGACAAAAT TGTGCCAGCT TGTGGAAGTA ATGATGAAGC GACGCGACGA CCTCGCCTTT
    CGCCAGGAGA TGAAGTTCCG GAATAAACTG GTTGAATACT TGACCGACTG GGTGATGGGC
    ACCTCCCATC AGATTGCACC GCCCAGCTCG GCGGATGCCG CTATTCTTAC CAACACATCC
    CTCATATTTC GCGATCTAGA CCAAGCCTGC ATGGAGGCAG TGGCTGCCTT GCTTCGGGGC
    CTTCCCCTTC AGCCTGAGGA ATCGGATCGA GGCGATTTGA TGGATGCAAA GAGTGCGCTC
    TTTTTAAAGT ACTTTACCCT ATTTATGAAC CTGCTGAATG ATTGCATCGA TAGCTCCGAG
    GCGGAGAAGG AAATGAATAA TACCCCACTA TTGCCTCCGC GCCCTCGAAT GGCAGCTGGA
    AAACTGACCG CTCTGCGAAA TGCCACTATT CTAGCCATGT CTAATTTGTT GGGTGCCAAC
    ATTGACTCTG GTTTGATGCA CTCCATCGAT CTGGGTTATA ATCCTGATTT GCAAACGCGT
    GCCGCTTTCA TGGAGGTGTT GACTCAAATC CTGCAACAAG GCACCGAATT CGATACCTTG
    GCCGAAACTG TTTTAGCTGA TCGATTTGAG CAACTGGTCC AACTTGTTAC AATGATCAGC
    GACAAAGGAG AACTTCCCAT AGCAATGGCT TTGGCTAATG TGGTGACTAC ATCCCAAATG
    GACGAGCTGG CTAGGGTTCT GGTCACCCTA TTCGACGCCA AACACCTGTT ATCGCCACTC
    CTATGGAATA TGTTTTATCG CGAGGTGGAG GTCTCAGACT GCATGCAGAC ACTTTTCCGT
    GGAAATTCTT TGGGCAGCAA AATCATGGCC TTTTGCTTTA AAATATACGG CGCGAGCTAT
    CTCCAAATGC TACTAGAGCC TTTAATTCGT CCGCTTCTGG ATGAGGAGGA GGAGACCTGT
    TTTGAGGTGG ATCCAGCTCG TCTGGATCCA ACAGAAGATA TTGAACAGCA CAGGAACAAC
    TTGATTGCCC TAACGCAGAA GGTGTTTGAC GCTATTATCA ACTCGTCGGA TCGCTTTCCC
    CCGCAGCTGC GATCCATGTG CCATTGCCTG TACCAAGTGC TAAGCAAACG CTTCCCGAAT
    CTTCTTCAAA ACAATATCGG TGCTGTAGGC ACAGTCATCT TCTTGCGGTT CATCAATCCC
    GCCATAGTTT CCCCACAGGA ATTGGGAATC GTTGACAAAC AAGTGCACAG CTCGGCCAAA
    CGAGGTCTAA TGCTAATGTC CAAGATCCTT CAAAACATTG CTAACCACGT GGAGTTCTCC
    AAGGAGCAGC ACATGTTGTG TTTCAACGAT TTTCTGCGCG ATCACTTTGA AGCTGGGCGG
    CGATTCTTCA TACAGATTGC ATCAGACTGT GAGACCGTGG ATCAGACCTC GCACAGCATG
    AGTTTTATTT CAGATGCGAA CGTACTGGCA CTGCATCGGT TGCTGTGGAC GCATCAGGAG
    AAGATCGGCG ACTATCTGTC TAGCAGTCGA GACCACAAGG CGGTTGGAAG GCGGCCCTTC
    GACAAGATGG CTACTTTGCT AGCATACCTG GGACCACCGG AGCACAAGCC CGTGGATTCG
    CACATGATGT TCAGCTCGTA TGCACGCTGG AGCTCCATTG ACATGTCGTC GACAAACTTC
    GAGGAGATCA TGGTCAAGCA CCAAATGCAC GAGAAGGAGG AATTCAAGAC CCTCAAGTCG
    ATGAACATAT TCTACCAGGC CGGAACAAGC AAATCTGGCT ATCCTGTCTT TTACTATATA
    GCAAGACGAT ACAAGATTGG AGAAACGAAT GGAGACTTAC TTATATATCA CGTCATACTC
    ACTCTGAAAC CATTCTGCCA CTCGCCCTTC GAGGTGGTCA TCGATTTTAC GCACACCTGT
    TCAGATAATC GGTTCCGCAC CGAGTTTCTG CAGAAGTGGT TTTATGTCCT GCCCACGGTA
    GCCTACGAAA ATGTCCATGC TGTGTACATC TATAACTGTA ACTCGTGGGT GCGCGAATAT
    ACCAAGTTCC ACGATCGCAT TCTGGCACCA TTAAAGGGAA ATCGCAAACT TCTGTTCCTG
    GAGTCACCCA ACAAACTTAC TGATTTCATC GACGCGGAGC AACAGAAATT GCCTGGAGCA
    ACTCTTTCCT TGGACGAAGA TTTAAAGGTA TTCAGCAACG CGCTGAAGCT CAGCCATAAA
    GACACAAAGG TGGCTATCAA AGTGGGTCCC ACCGCATTGC AAATTACATC TGCCGAAAAG
    ACAAAGGTCT TGGCTCACTC CGTGCTCCTG AACGATGTAT ACTACGCCTC TGAAATTGAG
    GAGGTGTGCT TGGTGGACGA TAACCAGTTC ACCCTGTCTA TAACTAACGA AAGTGGCCAG
    CTTAGCTTTA TTCACAACGA TTGCGACAAC ATTGTTCAAG CCATCATACA CATCCGAAAT
    CGATGGGAGC TTAGTCAGCC GGACTCCGTG ACGGTTCACC AAAAGATCCG ACCAAAAGAT
    GTGCCTGGAA CGCTGCTGAA CATGGCGCTA CTCAATCTGG GATCATGTGA CCCCAATCTA
    AGGACTGCTG CCTACAATTT GCTGTGTGCT TTGACCGCTA CTTTTGATCT AAAGATTGAG
    GGTCAACTGT TGGAGACCCA AGGACTTTGC ATACCCTCAA ATAATACCAT ATTTATCAAA
    TCCGTTAGCG AAAAGCTGGC CACCAATGAA CCGCATCTGA CTCTGGAATT CCTTGAGGAG
    TCCATTCAGG GCTTCCAGCG CAGCACCATA GAGCTGAAAC ATTTGTGTTT GGAGTACATG
    ACGCCATGGT TAAAGAACCT AGTCAAATTT TGCAAGTCCA ACGATGACTC CAAAAAGCTT
    AAGGTCTCCC AAATTCTGGA CAAGCTCATC AATTTAACTA TTGACCAAAA GGAAATGTAT
    CCCTCAGTGC AGGCCAAGAT CTGGGGATCA ATTGGACAGA TCCCCGAGCT GATCGACATG
    GTTTTGGATA ACTTTTTGCA CAAATCGATC ACATATGGCT TGGGATCACC ACAGGTGGAG
    ATTATGGCTG ACACGGCAGT GGCTCTCGCT TCAGCGAATG TTCAGCTGGT GTCCAAAAAG
    GTCATAACGC GAATATGCCG AGTCATGGAC AAATCCTGCA CAAATCCCAC ACAATATCTG
    GAGCAGCATA TGATGTGGGA CGATATTGCA ATTCTTGGTC GATACCTGCT CATGTTGTCG
    TTCAACAATT GTTTGGATGT GGCCACATCG GTGCCATATC TATTCCACAC CATTACGTTT
    TTGGTATGTT CAGGATCCCT GTCGATGCGA GCCTCTACTC ATGGCCTAGT GATCAACATC
    ATCCACTCGC TGTGCACATG CACAAATCCC TCATTTTCAG AGGAGGCGCA GCGAGTACTC
    CGACTGTCCC TGGATGAATT CTCACTGCCC AAGTTCTATC TACTCTTCGG CATCAGCAAG
    GTCAAGTCCG CAGCAGTTAC CGCTTTCCGC TCCAGCTGCC GTCACCCTAC AGATAAATGG
    CTAGGAAATG AAAGGGTTAC CCAGCCTCTG CCCGCCGATC GAGAGCGTTT ATCCCTACCA
    TCGCTTGAGG TTATCACGGA TGCCCTGCTG GAAATCATGG AGGCTTGCAT GCGAGATGTT
    CCGGACTGCG AATGGCTCAA CACCTGGACC TCTTTGGCTC GCAGTTTTGC TTTCTGTTAC
    AATCCAGCGC TACAGCCTAG AGCCCTTATA GTTTACGGGT GCATCAGCAA GAGTGTTACC
    GATCACGAGG TCAAGCAGTT ACTACGCATC CTGGTTAAGG CCCTCGAATC TTTCAACGAT
    CTCATTCTGA TCGAGGCTCT AGTAATGTGC TTAACTCGCA TTCAACCACT ACTCCGACCA
    GAGTCGCCCA TTCATCGGGC CCTCTTCTGG GTGGCCATTT CGGTGCTGCA GTTGGACGAG
    ATTACCCTGT ACGGCGCTGG ACTAGCTCTG CTTGAACAAA ATCTACACAC GCTTAAGTCA
    CAGGGCTGTT TTGACAAGAA GGAGACCATA GCCGAGGTCA TGATGAAGAC AAGGGAAAAG
    CTGGAGTGGC ATTTCAAGCA ACTGGATCAC GCCGTCGGCC TCTCCTTCCG CAGCAATTTC
    CACTTTGCCC TAGTGGGACA CTTGATTAAG GGCTTCCGCC ATCCTACACC TACAACCGTC
    TCACGCACCT CTCGTGTGCT GACGATGCTC TTGGGCATAA TAGCCAAACC CCTGCATCGG
    GACAAGTTCG AAGTAACACC TGACAGTGTG GCCTATTTGA CTGCGCTGGT GGCTGTCTCC
    GAGGAAGTCC GTTCCAGATG TCACGTAAAA CATGCCCTTC CCCGCTGGCC AGCCGATCTG
    AGTAGTAGTG TGGAAAATGG TGAAGCATCC GGCGGAGTGC AAGCTATCGG CCTGCCCCTG
    TCACGCCGTC AAAAAAGTTG GGACATCCTG GATCAGTCCG CCTTGCAGTT TGCCCGCCAA
    CACAAGGTTC CCACTCTTCA GAACGCGCGA GTTTTGTTCA AAACTCAACG CTCATTCTCG
    GTGCCGACGA CCAAAGATCC GAACAATGCC ACCGGCATCG AAGAACGTCA GGAACGCGGT
    TCGCGTTCGT CGGTGTCCAA CGAGTCCAAC GTACTGCTCG ATCCCGAGGT CTTACCTGAC
    CTGTCCATTC AAGCCCTGGT ACTGACTGTT TTGGCCACCT TGGTCAAGTA CTCTTCGGAT
    GAAGGCGAAA CGCGGGTTTT GTATCAGTAT TTGGCCGAAG GATCGGTGGT CTTTCCAAAG
    GTGTTTCCAG TTATCCATTC ACTGCTGGAC CAAAAGATAA ACAACATTTT GTCAGTGTCA
    CACGACCAAG TGGTGCTCAA CTCTGTGCAG AACATTATAC AAAATATGCT GGCCAGCGAA
    GATCCTTCCC AGCAGCAGCT GCACTTCCTG CAGAGTTGTG GATTTGGCGG ACTCTGGCGA
    TTTGCTGGTC CCTTCACCAA GTACAATATG ATGGGTGAGT CCTCGGAGCT GTTTGTCAAC
    TGTTTGGAGG CCATGGTTGA GACCTGTTTG CCAGGCGACG AATCCGCGCC GGTGCCACCA
    TCTCCGCGTC CATACAACCT GAGCTCCAGT CTGAGTAGCC TGACCCTGGG ATCACCCACG
    GATAAAGCTG CATGAATAAC AACTACAAAC CCGCGCACTA GACGTACCAC ACTTATCGCA
    AAAGTAATGC GTTTCCTAAA TCTGCTTAAA AAGTGCTTTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08850
    Clone ID Related Accession (Same CDS sequence) NM_001276050.1
    Accession Version NM_001276050.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product neurofibromin 1, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGACCCAGA AGCCAGGCGA GTGGGCTAGC GCCCTTTTGG CACGTTTCGA GGATCAGCTG 
    CCAAACAGAA TCGGTGCCTA TGGCACGCAG GCCAGGATGA GCCAGGACCA ATTGGTGGCC
    TGTCTGATCC ACATATCGCG CTACCGCTTC TCCCTTGTCA TATCCGGTCT CACCAAGATG
    CTGCAGAGGG TCAATGAGGC GGCCCTTCAA AATCGCCATG AGCCAGAGCG TTGCTACTTT
    GAGTCGCTGG TCATCATACT GACCACTCTG GAACGCTGTC TGACCAACCA GACCAAGGAC
    ACAGCACGTT TTGAAGAGGC CATGAATGTG AAGCTTCTGC TGCGCGAGAT CTCCCAGTTT
    GTCGACGTCC AGAGCGACAG CAATCCGAAT GCCGCCCAGC TCAAGGCCCT GGCATCCAAG
    GTGCTCTTTG CCTTGTCCCA GAATCACTTC TCGGCGGTGT TCAATCGCAT ATCGGCCAGG
    ATTCAAGAGC TGACATCCTG TTCCGAGGAA AACCCGGACT ACAACGACAT AGAATTAATT
    CAACACATCG ATATGGATAT GATAAAGCTA ACCAAGCTGC TACAAGAAAC CATCACAAAG
    TTTCGATCGA AGCGTGCTCC ACCTTTGATC TTGCTTTACT CGCTGGAGAA GGCTATTTGG
    AACTGGATCG AGTACCATCC ACAAGAGTTC CAGGACTTGC AACGAGGAAC CAATCGAGAT
    ATATCAACCT GCTGGGAACC CCTGATGGAC TTTGTAGAGT ACTTTAAAAC CGAAAACAAG
    AAAAGCAAGA CTCTTGTTTG GCCGCTTCAA ATGCTCTTGC TGATATTGAA TCCCTCTTGC
    CTTGAGGCTG TTGTTAACGA ACTTCAGCAG TCGGAGAAAG AGAAGGAAAA GGACAAGGAA
    AAGGTTGCCT CGAAGTCAGC GCAATCCACG TCCCGGGACA AGGACTTCTC GGCCAAGCAG
    TTCATCGAGA GTATCAAGAG AGGCTTGGGC CAGCACTCAC CATCCAAACA GGTGACCGAA
    TCTGCGGCAA TTGCCTGTGT AAAGCTGTGC AAAGCATCCA CCTACATCAA CAACACCGAC
    TCCAATAATG TGGTCTTCAA GTTGGTGCAA TTCTTTATCA ACGATCTCAA GGCACTGCTC
    TTTAATCCAG CAAAGCCCTT TTCGCGCGGT CAGGGGTACA ACTTCGCTGA TATCGAGCTG
    ATGATTGACT GCTGGGTGTC TTGTTTCCGG ATTAATCCTC ACAACATCGA GGCTCTAAAG
    GTTTGCCTGA ATTTGTCCTC GCCGCAGGCT TACCATTTTG TAATTGTGTG CTCTCTGCTA
    AGGTTGGCTC ACATATACGT TGACTTCCGT TTGCAGAACA AGAACCCCTT TCGAATAGTA
    AACCAACCGA GATTGTCTTG GTGGCCGCAA ACGGATGTGG TTCACTACCG TTCTGCTGAG
    CTACGAGCTT TGTTTACGGA TACGCTCAAC AAGGCCACCC AGGGTTATAT AGCCCATACG
    CCACTGCGCT ACATAACCTC CCTGACGCTC AAATCTAAAG ATACCCAGAA AGGTTTAACC
    CGCGCTGAAG AGGGTCCCGC CCACAAAATG CTATTACTGC TACTCGTACG GCTAATTCAT
    GCAGATCCCA CGCTTTTGTT GAATACCCAA GGAAAAGTGG CACATGAAGT GCAGAGCTCC
    ACATTGGAGC TCATCAACGG CTTAGTTAGT TTGGTGCATC AAACTACCAT GCCCGATGTG
    GCACAAGAGG CTATGGAGGC GCTGCTTGCT CTGCACGCCC CGGAGAAAAT AGAGGTTTGG
    AATCCAGAGG CTCCGATCAA TACGTTCTGG GACGTTAGCT CCCAAGTACT GTTTTCAATC
    TCGCAAAAGC TCATTCAACA CCAAATTGCC AATTACACTG ATGTCTTAAA GTGGCTACGC
    GAGATCCTCA TCTGTCGGAA TACTTTTCTT CAGCGACACA AGGATTATGC ACATGTGGGA
    AGTCAGATTG CCATTTGCAA GCAGGCTCAC ATTAAGATGG AAGTTGTTTT CTTCATGTAC
    CTGTGGAGTG TTGATTTAGA TGCGGTGCTG ACATCGCTAT CGTGTTTTGG ACTACTATGC
    GAGGAGGCTG AGATTTGCTG CAGTTCTGAT GAATTGACAG TCGGATTTAT TATGCCGAAT
    TACCACATTT ACCAGGAGCT GGCTCAGTTG TCCACATCTG CAACGGACTC TCGCATTTGC
    TTTTTTGACA ACACGCACGG CAATGTGCTG AGCCGTCTTA CACTTCAAAA GCGCATAATG
    ACCCTATTGC GCAAGATCGA GCACTGTGTT CATGGTGTTC AGCCCGCCTG GGAGGAAACC
    TTCCGCAACT GGGAAGTATC CAGCAAGGTT TTGCAAACGT ACCCCAAATG TAAAGGAGAA
    GATGGCCAAG CGGAGGTTTT CCATCGTGGC ATGGGCAAGC GGCGAGCGAG TCACCAAAGC
    TCAGAGCACG ATTTGGAGGA ACAAATTAAT GAATGGGCCA ATATGACATG GTTTCTATTA
    GCCTTGGGTG GCGTCTGCCT TCATAAACGT AGCAGCAGTC GTCAAATGCT GCTGCAGCAA
    TCACAGAACA ATGCTTCTTT GGGATCACTT GCTCAAAACT CTCTTTACTC AAGCTCCACA
    AGTTCTGGAC ATGGCTCTCT GCATCCTAGC ACGGTTTCAC TATCCACTCT TCCCCCAGCA
    CCGCCACAAG ATGTCAGCTA TTGTCCTGTA ACACAATTCG TGGGCCAACT ATTGCGCCTG
    TTAGTTTGCA GCAACGAAAA AATTGGACTT AATATTCAGA AAAATGTGAA GGAACTGGTG
    GGCGAGGAGA TGTCCACCCA ACTGTATCCT ATACTCTTCG ACCAGGTTAG AGCCATCGTG
    GAGAAGTTCT TCGATCAGCA AGGTCAGGTG AACGTCAATG TGACCGACAT CAACACGCAG
    TTTATCGAGC ACACCATCTA CATAATGAAA TCAATTCTGG ATCCCAAAGC CAACAAGGAT
    CCCAACAACG ATCAACCCTC GCCATCGGAA CATTTAGGTG TTACAAGCAT CGAAGGCATG
    ATGCTAGGAA TAGTGCGCTA CGTTCGCCAC CTGGATATGA CTGTTTATGC CATTCGAATC
    AAGACAAAAT TGTGCCAGCT TGTGGAAGTA ATGATGAAGC GACGCGACGA CCTCGCCTTT
    CGCCAGGAGA TGAAGTTCCG GAATAAACTG GTTGAATACT TGACCGACTG GGTGATGGGC
    ACCTCCCATC AGATTGCACC GCCCAGCTCG GCGGATGCCG CTATTCTTAC CAACACATCC
    CTCATATTTC GCGATCTAGA CCAAGCCTGC ATGGAGGCAG TGGCTGCCTT GCTTCGGGGC
    CTTCCCCTTC AGCCTGAGGA ATCGGATCGA GGCGATTTGA TGGATGCAAA GAGTGCGCTC
    TTTTTAAAGT ACTTTACCCT ATTTATGAAC CTGCTGAATG ATTGCATCGA TAGCTCCGAG
    GCGGAGAAGG AAATGAATAA TACCCCACTA TTGCCTCCGC GCCCTCGAAT GGCAGCTGGA
    AAACTGACCG CTCTGCGAAA TGCCACTATT CTAGCCATGT CTAATTTGTT GGGTGCCAAC
    ATTGACTCTG GTTTGATGCA CTCCATCGAT CTGGGTTATA ATCCTGATTT GCAAACGCGT
    GCCGCTTTCA TGGAGGTGTT GACTCAAATC CTGCAACAAG GCACCGAATT CGATACCTTG
    GCCGAAACTG TTTTAGCTGA TCGATTTGAG CAACTGGTCC AACTTGTTAC AATGATCAGC
    GACAAAGGAG AACTTCCCAT AGCAATGGCT TTGGCTAATG TGGTGACTAC ATCCCAAATG
    GACGAGCTGG CTAGGGTTCT GGTCACCCTA TTCGACGCCA AACACCTGTT ATCGCCACTC
    CTATGGAATA TGTTTTATCG CGAGGTGGAG GTCTCAGACT GCATGCAGAC ACTTTTCCGT
    GGAAATTCTT TGGGCAGCAA AATCATGGCC TTTTGCTTTA AAATATACGG CGCGAGCTAT
    CTCCAAATGC TACTAGAGCC TTTAATTCGT CCGCTTCTGG ATGAGGAGGA GGAGACCTGT
    TTTGAGGTGG ATCCAGCTCG TCTGGATCCA ACAGAAGATA TTGAACAGCA CAGGAACAAC
    TTGATTGCCC TAACGCAGAA GGTGTTTGAC GCTATTATCA ACTCGTCGGA TCGCTTTCCC
    CCGCAGCTGC GATCCATGTG CCATTGCCTG TACCAAGTGC TAAGCAAACG CTTCCCGAAT
    CTTCTTCAAA ACAATATCGG TGCTGTAGGC ACAGTCATCT TCTTGCGGTT CATCAATCCC
    GCCATAGTTT CCCCACAGGA ATTGGGAATC GTTGACAAAC AAGTGCACAG CTCGGCCAAA
    CGAGGTCTAA TGCTAATGTC CAAGATCCTT CAAAACATTG CTAACCACGT GGAGTTCTCC
    AAGGAGCAGC ACATGTTGTG TTTCAACGAT TTTCTGCGCG ATCACTTTGA AGCTGGGCGG
    CGATTCTTCA TACAGATTGC ATCAGACTGT GAGACCGTGG ATCAGACCTC GCACAGCATG
    AGTTTTATTT CAGATGCGAA CGTACTGGCA CTGCATCGGT TGCTGTGGAC GCATCAGGAG
    AAGATCGGCG ACTATCTGTC TAGCAGTCGA GACCACAAGG CGGTTGGAAG GCGGCCCTTC
    GACAAGATGG CTACTTTGCT AGCATACCTG GGACCACCGG AGCACAAGCC CGTGGATTCG
    CACATGATGT TCAGCTCGTA TGCACGCTGG AGCTCCATTG ACATGTCGTC GACAAACTTC
    GAGGAGATCA TGGTCAAGCA CCAAATGCAC GAGAAGGAGG AATTCAAGAC CCTCAAGTCG
    ATGAACATAT TCTACCAGGC CGGAACAAGC AAATCTGGCT ATCCTGTCTT TTACTATATA
    GCAAGACGAT ACAAGATTGG AGAAACGAAT GGAGACTTAC TTATATATCA CGTCATACTC
    ACTCTGAAAC CATTCTGCCA CTCGCCCTTC GAGGTGGTCA TCGATTTTAC GCACACCTGT
    TCAGATAATC GGTTCCGCAC CGAGTTTCTG CAGAAGTGGT TTTATGTCCT GCCCACGGTA
    GCCTACGAAA ATGTCCATGC TGTGTACATC TATAACTGTA ACTCGTGGGT GCGCGAATAT
    ACCAAGTTCC ACGATCGCAT TCTGGCACCA TTAAAGGGAA ATCGCAAACT TCTGTTCCTG
    GAGTCACCCA ACAAACTTAC TGATTTCATC GACGCGGAGC AACAGAAATT GCCTGGAGCA
    ACTCTTTCCT TGGACGAAGA TTTAAAGGTA TTCAGCAACG CGCTGAAGCT CAGCCATAAA
    GACACAAAGG TGGCTATCAA AGTGGGTCCC ACCGCATTGC AAATTACATC TGCCGAAAAG
    ACAAAGGTCT TGGCTCACTC CGTGCTCCTG AACGATGTAT ACTACGCCTC TGAAATTGAG
    GAGGTGTGCT TGGTGGACGA TAACCAGTTC ACCCTGTCTA TAACTAACGA AAGTGGCCAG
    CTTAGCTTTA TTCACAACGA TTGCGACAAC ATTGTTCAAG CCATCATACA CATCCGAAAT
    CGATGGGAGC TTAGTCAGCC GGACTCCGTG ACGGTTCACC AAAAGATCCG ACCAAAAGAT
    GTGCCTGGAA CGCTGCTGAA CATGGCGCTA CTCAATCTGG GATCATGTGA CCCCAATCTA
    AGGACTGCTG CCTACAATTT GCTGTGTGCT TTGACCGCTA CTTTTGATCT AAAGATTGAG
    GGTCAACTGT TGGAGACCCA AGGACTTTGC ATACCCTCAA ATAATACCAT ATTTATCAAA
    TCCGTTAGCG AAAAGCTGGC CACCAATGAA CCGCATCTGA CTCTGGAATT CCTTGAGGAG
    TCCATTCAGG GCTTCCAGCG CAGCACCATA GAGCTGAAAC ATTTGTGTTT GGAGTACATG
    ACGCCATGGT TAAAGAACCT AGTCAAATTT TGCAAGTCCA ACGATGACTC CAAAAAGCTT
    AAGGTCTCCC AAATTCTGGA CAAGCTCATC AATTTAACTA TTGACCAAAA GGAAATGTAT
    CCCTCAGTGC AGGCCAAGAT CTGGGGATCA ATTGGACAGA TCCCCGAGCT GATCGACATG
    GTTTTGGATA ACTTTTTGCA CAAATCGATC ACATATGGCT TGGGATCACC ACAGGTGGAG
    ATTATGGCTG ACACGGCAGT GGCTCTCGCT TCAGCGAATG TTCAGCTGGT GTCCAAAAAG
    GTCATAACGC GAATATGCCG AGTCATGGAC AAATCCTGCA CAAATCCCAC ACAATATCTG
    GAGCAGCATA TGATGTGGGA CGATATTGCA ATTCTTGGTC GATACCTGCT CATGTTGTCG
    TTCAACAATT GTTTGGATGT GGCCACATCG GTGCCATATC TATTCCACAC CATTACGTTT
    TTGGTATGTT CAGGATCCCT GTCGATGCGA GCCTCTACTC ATGGCCTAGT GATCAACATC
    ATCCACTCGC TGTGCACATG CACAAATCCC TCATTTTCAG AGGAGGCGCA GCGAGTACTC
    CGACTGTCCC TGGATGAATT CTCACTGCCC AAGTTCTATC TACTCTTCGG CATCAGCAAG
    GTCAAGTCCG CAGCAGTTAC CGCTTTCCGC TCCAGCTGCC GTCACCCTAC AGATAAATGG
    CTAGGAAATG AAAGGGTTAC CCAGCCTCTG CCCGCCGATC GAGAGCGTTT ATCCCTACCA
    TCGCTTGAGG TTATCACGGA TGCCCTGCTG GAAATCATGG AGGCTTGCAT GCGAGATGTT
    CCGGACTGCG AATGGCTCAA CACCTGGACC TCTTTGGCTC GCAGTTTTGC TTTCTGTTAC
    AATCCAGCGC TACAGCCTAG AGCCCTTATA GTTTACGGGT GCATCAGCAA GAGTGTTACC
    GATCACGAGG TCAAGCAGTT ACTACGCATC CTGGTTAAGG CCCTCGAATC TTTCAACGAT
    CTCATTCTGA TCGAGGCTCT AGTAATGTGC TTAACTCGCA TTCAACCACT ACTCCGACCA
    GAGTCGCCCA TTCATCGGGC CCTCTTCTGG GTGGCCATTT CGGTGCTGCA GTTGGACGAG
    ATTACCCTGT ACGGCGCTGG ACTAGCTCTG CTTGAACAAA ATCTACACAC GCTTAAGTCA
    CAGGGCTGTT TTGACAAGAA GGAGACCATA GCCGAGGTCA TGATGAAGAC AAGGGAAAAG
    CTGGAGTGGC ATTTCAAGCA ACTGGATCAC GCCGTCGGCC TCTCCTTCCG CAGCAATTTC
    CACTTTGCCC TAGTGGGACA CTTGATTAAG GGCTTCCGCC ATCCTACACC TACAACCGTC
    TCACGCACCT CTCGTGTGCT GACGATGCTC TTGGGCATAA TAGCCAAACC CCTGCATCGG
    GACAAGTTCG AAGTAACACC TGACAGTGTG GCCTATTTGA CTGCGCTGGT GGCTGTCTCC
    GAGGAAGTCC GTTCCAGATG TCACGTAAAA CATGCCCTTC CCCGCTGGCC AGCCGATCTG
    AGTAGTAGTG TGGAAAATGG TGAAGCATCC GGCGGAGTGC AAGCTATCGG CCTGCCCCTG
    TCACGCCGTC AAAAAAGTTG GGACATCCTG GATCAGTCCG CCTTGCAGTT TGCCCGCCAA
    CACAAGGTTC CCACTCTTCA GGAACGCGGT TCGCGTTCGT CGGTGTCCAA CGAGTCCAAC
    GTACTGCTCG ATCCCGAGGT CTTACCTGAC CTGTCCATTC AAGCCCTGGT ACTGACTGTT
    TTGGCCACCT TGGTCAAGTA CTCTTCGGAT GAAGGCGAAA CGCGGGTTTT GTATCAGTAT
    TTGGCCGAAG GATCGGTGGT CTTTCCAAAG GTGTTTCCAG TTATCCATTC ACTGCTGGAC
    CAAAAGATAA ACAACATTTT GTCAGTGTCA CACGACCAAG TGGTGCTCAA CTCTGTGCAG
    AACATTATAC AAAATATGCT GGCCAGCGAA GATCCTTCCC AGCAGCAGCT GCACTTCCTG
    CAGAGTTGTG GATTTGGCGG ACTCTGGCGA TTTGCTGGTC CCTTCACCAA GTACAATATG
    ATGGGTGAGT CCTCGGAGCT GTTTGTCAAC TGTTTGGAGG CCATGGTTGA GACCTGTTTG
    CCAGGCGACG AATCCGCGCC GGTGCCACCA TCTCCGCGTC CATACAACCT GAGCTCCAGT
    CTGAGTAGCC TGACCCTGGG ATCACCCACG GATAAAGCTG CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001262979.1
    CDS280..8484
    Translation

    Target ORF information:

    RefSeq Version NM_001276050.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster neurofibromin 1, transcript variant F (Nf1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001276050.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    ATGACCCAGA AGCCAGGCGA GTGGGCTAGC GCCCTTTTGG CACGTTTCGA GGATCAGCTG 
    CCAAACAGAA TCGGTGCCTA TGGCACGCAG GCCAGGATGA GCCAGGACCA ATTGGTGGCC
    TGTCTGATCC ACATATCGCG CTACCGCTTC TCCCTTGTCA TATCCGGTCT CACCAAGATG
    CTGCAGAGGG TCAATGAGGC GGCCCTTCAA AATCGCCATG AGCCAGAGCG TTGCTACTTT
    GAGTCGCTGG TCATCATACT GACCACTCTG GAACGCTGTC TGACCAACCA GACCAAGGAC
    ACAGCACGTT TTGAAGAGGC CATGAATGTG AAGCTTCTGC TGCGCGAGAT CTCCCAGTTT
    GTCGACGTCC AGAGCGACAG CAATCCGAAT GCCGCCCAGC TCAAGGCCCT GGCATCCAAG
    GTGCTCTTTG CCTTGTCCCA GAATCACTTC TCGGCGGTGT TCAATCGCAT ATCGGCCAGG
    ATTCAAGAGC TGACATCCTG TTCCGAGGAA AACCCGGACT ACAACGACAT AGAATTAATT
    CAACACATCG ATATGGATAT GATAAAGCTA ACCAAGCTGC TACAAGAAAC CATCACAAAG
    TTTCGATCGA AGCGTGCTCC ACCTTTGATC TTGCTTTACT CGCTGGAGAA GGCTATTTGG
    AACTGGATCG AGTACCATCC ACAAGAGTTC CAGGACTTGC AACGAGGAAC CAATCGAGAT
    ATATCAACCT GCTGGGAACC CCTGATGGAC TTTGTAGAGT ACTTTAAAAC CGAAAACAAG
    AAAAGCAAGA CTCTTGTTTG GCCGCTTCAA ATGCTCTTGC TGATATTGAA TCCCTCTTGC
    CTTGAGGCTG TTGTTAACGA ACTTCAGCAG TCGGAGAAAG AGAAGGAAAA GGACAAGGAA
    AAGGTTGCCT CGAAGTCAGC GCAATCCACG TCCCGGGACA AGGACTTCTC GGCCAAGCAG
    TTCATCGAGA GTATCAAGAG AGGCTTGGGC CAGCACTCAC CATCCAAACA GGTGACCGAA
    TCTGCGGCAA TTGCCTGTGT AAAGCTGTGC AAAGCATCCA CCTACATCAA CAACACCGAC
    TCCAATAATG TGGTCTTCAA GTTGGTGCAA TTCTTTATCA ACGATCTCAA GGCACTGCTC
    TTTAATCCAG CAAAGCCCTT TTCGCGCGGT CAGGGGTACA ACTTCGCTGA TATCGAGCTG
    ATGATTGACT GCTGGGTGTC TTGTTTCCGG ATTAATCCTC ACAACATCGA GGCTCTAAAG
    GTTTGCCTGA ATTTGTCCTC GCCGCAGGCT TACCATTTTG TAATTGTGTG CTCTCTGCTA
    AGGTTGGCTC ACATATACGT TGACTTCCGT TTGCAGAACA AGAACCCCTT TCGAATAGTA
    AACCAACCGA GATTGTCTTG GTGGCCGCAA ACGGATGTGG TTCACTACCG TTCTGCTGAG
    CTACGAGCTT TGTTTACGGA TACGCTCAAC AAGGCCACCC AGGGTTATAT AGCCCATACG
    CCACTGCGCT ACATAACCTC CCTGACGCTC AAATCTAAAG ATACCCAGAA AGGTTTAACC
    CGCGCTGAAG AGGGTCCCGC CCACAAAATG CTATTACTGC TACTCGTACG GCTAATTCAT
    GCAGATCCCA CGCTTTTGTT GAATACCCAA GGAAAAGTGG CACATGAAGT GCAGAGCTCC
    ACATTGGAGC TCATCAACGG CTTAGTTAGT TTGGTGCATC AAACTACCAT GCCCGATGTG
    GCACAAGAGG CTATGGAGGC GCTGCTTGCT CTGCACGCCC CGGAGAAAAT AGAGGTTTGG
    AATCCAGAGG CTCCGATCAA TACGTTCTGG GACGTTAGCT CCCAAGTACT GTTTTCAATC
    TCGCAAAAGC TCATTCAACA CCAAATTGCC AATTACACTG ATGTCTTAAA GTGGCTACGC
    GAGATCCTCA TCTGTCGGAA TACTTTTCTT CAGCGACACA AGGATTATGC ACATGTGGGA
    AGTCAGATTG CCATTTGCAA GCAGGCTCAC ATTAAGATGG AAGTTGTTTT CTTCATGTAC
    CTGTGGAGTG TTGATTTAGA TGCGGTGCTG ACATCGCTAT CGTGTTTTGG ACTACTATGC
    GAGGAGGCTG AGATTTGCTG CAGTTCTGAT GAATTGACAG TCGGATTTAT TATGCCGAAT
    TACCACATTT ACCAGGAGCT GGCTCAGTTG TCCACATCTG CAACGGACTC TCGCATTTGC
    TTTTTTGACA ACACGCACGG CAATGTGCTG AGCCGTCTTA CACTTCAAAA GCGCATAATG
    ACCCTATTGC GCAAGATCGA GCACTGTGTT CATGGTGTTC AGCCCGCCTG GGAGGAAACC
    TTCCGCAACT GGGAAGTATC CAGCAAGGTT TTGCAAACGT ACCCCAAATG TAAAGGAGAA
    GATGGCCAAG CGGAGGTTTT CCATCGTGGC ATGGGCAAGC GGCGAGCGAG TCACCAAAGC
    TCAGAGCACG ATTTGGAGGA ACAAATTAAT GAATGGGCCA ATATGACATG GTTTCTATTA
    GCCTTGGGTG GCGTCTGCCT TCATAAACGT AGCAGCAGTC GTCAAATGCT GCTGCAGCAA
    TCACAGAACA ATGCTTCTTT GGGATCACTT GCTCAAAACT CTCTTTACTC AAGCTCCACA
    AGTTCTGGAC ATGGCTCTCT GCATCCTAGC ACGGTTTCAC TATCCACTCT TCCCCCAGCA
    CCGCCACAAG ATGTCAGCTA TTGTCCTGTA ACACAATTCG TGGGCCAACT ATTGCGCCTG
    TTAGTTTGCA GCAACGAAAA AATTGGACTT AATATTCAGA AAAATGTGAA GGAACTGGTG
    GGCGAGGAGA TGTCCACCCA ACTGTATCCT ATACTCTTCG ACCAGGTTAG AGCCATCGTG
    GAGAAGTTCT TCGATCAGCA AGGTCAGGTG AACGTCAATG TGACCGACAT CAACACGCAG
    TTTATCGAGC ACACCATCTA CATAATGAAA TCAATTCTGG ATCCCAAAGC CAACAAGGAT
    CCCAACAACG ATCAACCCTC GCCATCGGAA CATTTAGGTG TTACAAGCAT CGAAGGCATG
    ATGCTAGGAA TAGTGCGCTA CGTTCGCCAC CTGGATATGA CTGTTTATGC CATTCGAATC
    AAGACAAAAT TGTGCCAGCT TGTGGAAGTA ATGATGAAGC GACGCGACGA CCTCGCCTTT
    CGCCAGGAGA TGAAGTTCCG GAATAAACTG GTTGAATACT TGACCGACTG GGTGATGGGC
    ACCTCCCATC AGATTGCACC GCCCAGCTCG GCGGATGCCG CTATTCTTAC CAACACATCC
    CTCATATTTC GCGATCTAGA CCAAGCCTGC ATGGAGGCAG TGGCTGCCTT GCTTCGGGGC
    CTTCCCCTTC AGCCTGAGGA ATCGGATCGA GGCGATTTGA TGGATGCAAA GAGTGCGCTC
    TTTTTAAAGT ACTTTACCCT ATTTATGAAC CTGCTGAATG ATTGCATCGA TAGCTCCGAG
    GCGGAGAAGG AAATGAATAA TACCCCACTA TTGCCTCCGC GCCCTCGAAT GGCAGCTGGA
    AAACTGACCG CTCTGCGAAA TGCCACTATT CTAGCCATGT CTAATTTGTT GGGTGCCAAC
    ATTGACTCTG GTTTGATGCA CTCCATCGAT CTGGGTTATA ATCCTGATTT GCAAACGCGT
    GCCGCTTTCA TGGAGGTGTT GACTCAAATC CTGCAACAAG GCACCGAATT CGATACCTTG
    GCCGAAACTG TTTTAGCTGA TCGATTTGAG CAACTGGTCC AACTTGTTAC AATGATCAGC
    GACAAAGGAG AACTTCCCAT AGCAATGGCT TTGGCTAATG TGGTGACTAC ATCCCAAATG
    GACGAGCTGG CTAGGGTTCT GGTCACCCTA TTCGACGCCA AACACCTGTT ATCGCCACTC
    CTATGGAATA TGTTTTATCG CGAGGTGGAG GTCTCAGACT GCATGCAGAC ACTTTTCCGT
    GGAAATTCTT TGGGCAGCAA AATCATGGCC TTTTGCTTTA AAATATACGG CGCGAGCTAT
    CTCCAAATGC TACTAGAGCC TTTAATTCGT CCGCTTCTGG ATGAGGAGGA GGAGACCTGT
    TTTGAGGTGG ATCCAGCTCG TCTGGATCCA ACAGAAGATA TTGAACAGCA CAGGAACAAC
    TTGATTGCCC TAACGCAGAA GGTGTTTGAC GCTATTATCA ACTCGTCGGA TCGCTTTCCC
    CCGCAGCTGC GATCCATGTG CCATTGCCTG TACCAAGTGC TAAGCAAACG CTTCCCGAAT
    CTTCTTCAAA ACAATATCGG TGCTGTAGGC ACAGTCATCT TCTTGCGGTT CATCAATCCC
    GCCATAGTTT CCCCACAGGA ATTGGGAATC GTTGACAAAC AAGTGCACAG CTCGGCCAAA
    CGAGGTCTAA TGCTAATGTC CAAGATCCTT CAAAACATTG CTAACCACGT GGAGTTCTCC
    AAGGAGCAGC ACATGTTGTG TTTCAACGAT TTTCTGCGCG ATCACTTTGA AGCTGGGCGG
    CGATTCTTCA TACAGATTGC ATCAGACTGT GAGACCGTGG ATCAGACCTC GCACAGCATG
    AGTTTTATTT CAGATGCGAA CGTACTGGCA CTGCATCGGT TGCTGTGGAC GCATCAGGAG
    AAGATCGGCG ACTATCTGTC TAGCAGTCGA GACCACAAGG CGGTTGGAAG GCGGCCCTTC
    GACAAGATGG CTACTTTGCT AGCATACCTG GGACCACCGG AGCACAAGCC CGTGGATTCG
    CACATGATGT TCAGCTCGTA TGCACGCTGG AGCTCCATTG ACATGTCGTC GACAAACTTC
    GAGGAGATCA TGGTCAAGCA CCAAATGCAC GAGAAGGAGG AATTCAAGAC CCTCAAGTCG
    ATGAACATAT TCTACCAGGC CGGAACAAGC AAATCTGGCT ATCCTGTCTT TTACTATATA
    GCAAGACGAT ACAAGATTGG AGAAACGAAT GGAGACTTAC TTATATATCA CGTCATACTC
    ACTCTGAAAC CATTCTGCCA CTCGCCCTTC GAGGTGGTCA TCGATTTTAC GCACACCTGT
    TCAGATAATC GGTTCCGCAC CGAGTTTCTG CAGAAGTGGT TTTATGTCCT GCCCACGGTA
    GCCTACGAAA ATGTCCATGC TGTGTACATC TATAACTGTA ACTCGTGGGT GCGCGAATAT
    ACCAAGTTCC ACGATCGCAT TCTGGCACCA TTAAAGGGAA ATCGCAAACT TCTGTTCCTG
    GAGTCACCCA ACAAACTTAC TGATTTCATC GACGCGGAGC AACAGAAATT GCCTGGAGCA
    ACTCTTTCCT TGGACGAAGA TTTAAAGGTA TTCAGCAACG CGCTGAAGCT CAGCCATAAA
    GACACAAAGG TGGCTATCAA AGTGGGTCCC ACCGCATTGC AAATTACATC TGCCGAAAAG
    ACAAAGGTCT TGGCTCACTC CGTGCTCCTG AACGATGTAT ACTACGCCTC TGAAATTGAG
    GAGGTGTGCT TGGTGGACGA TAACCAGTTC ACCCTGTCTA TAACTAACGA AAGTGGCCAG
    CTTAGCTTTA TTCACAACGA TTGCGACAAC ATTGTTCAAG CCATCATACA CATCCGAAAT
    CGATGGGAGC TTAGTCAGCC GGACTCCGTG ACGGTTCACC AAAAGATCCG ACCAAAAGAT
    GTGCCTGGAA CGCTGCTGAA CATGGCGCTA CTCAATCTGG GATCATGTGA CCCCAATCTA
    AGGACTGCTG CCTACAATTT GCTGTGTGCT TTGACCGCTA CTTTTGATCT AAAGATTGAG
    GGTCAACTGT TGGAGACCCA AGGACTTTGC ATACCCTCAA ATAATACCAT ATTTATCAAA
    TCCGTTAGCG AAAAGCTGGC CACCAATGAA CCGCATCTGA CTCTGGAATT CCTTGAGGAG
    TCCATTCAGG GCTTCCAGCG CAGCACCATA GAGCTGAAAC ATTTGTGTTT GGAGTACATG
    ACGCCATGGT TAAAGAACCT AGTCAAATTT TGCAAGTCCA ACGATGACTC CAAAAAGCTT
    AAGGTCTCCC AAATTCTGGA CAAGCTCATC AATTTAACTA TTGACCAAAA GGAAATGTAT
    CCCTCAGTGC AGGCCAAGAT CTGGGGATCA ATTGGACAGA TCCCCGAGCT GATCGACATG
    GTTTTGGATA ACTTTTTGCA CAAATCGATC ACATATGGCT TGGGATCACC ACAGGTGGAG
    ATTATGGCTG ACACGGCAGT GGCTCTCGCT TCAGCGAATG TTCAGCTGGT GTCCAAAAAG
    GTCATAACGC GAATATGCCG AGTCATGGAC AAATCCTGCA CAAATCCCAC ACAATATCTG
    GAGCAGCATA TGATGTGGGA CGATATTGCA ATTCTTGGTC GATACCTGCT CATGTTGTCG
    TTCAACAATT GTTTGGATGT GGCCACATCG GTGCCATATC TATTCCACAC CATTACGTTT
    TTGGTATGTT CAGGATCCCT GTCGATGCGA GCCTCTACTC ATGGCCTAGT GATCAACATC
    ATCCACTCGC TGTGCACATG CACAAATCCC TCATTTTCAG AGGAGGCGCA GCGAGTACTC
    CGACTGTCCC TGGATGAATT CTCACTGCCC AAGTTCTATC TACTCTTCGG CATCAGCAAG
    GTCAAGTCCG CAGCAGTTAC CGCTTTCCGC TCCAGCTGCC GTCACCCTAC AGATAAATGG
    CTAGGAAATG AAAGGGTTAC CCAGCCTCTG CCCGCCGATC GAGAGCGTTT ATCCCTACCA
    TCGCTTGAGG TTATCACGGA TGCCCTGCTG GAAATCATGG AGGCTTGCAT GCGAGATGTT
    CCGGACTGCG AATGGCTCAA CACCTGGACC TCTTTGGCTC GCAGTTTTGC TTTCTGTTAC
    AATCCAGCGC TACAGCCTAG AGCCCTTATA GTTTACGGGT GCATCAGCAA GAGTGTTACC
    GATCACGAGG TCAAGCAGTT ACTACGCATC CTGGTTAAGG CCCTCGAATC TTTCAACGAT
    CTCATTCTGA TCGAGGCTCT AGTAATGTGC TTAACTCGCA TTCAACCACT ACTCCGACCA
    GAGTCGCCCA TTCATCGGGC CCTCTTCTGG GTGGCCATTT CGGTGCTGCA GTTGGACGAG
    ATTACCCTGT ACGGCGCTGG ACTAGCTCTG CTTGAACAAA ATCTACACAC GCTTAAGTCA
    CAGGGCTGTT TTGACAAGAA GGAGACCATA GCCGAGGTCA TGATGAAGAC AAGGGAAAAG
    CTGGAGTGGC ATTTCAAGCA ACTGGATCAC GCCGTCGGCC TCTCCTTCCG CAGCAATTTC
    CACTTTGCCC TAGTGGGACA CTTGATTAAG GGCTTCCGCC ATCCTACACC TACAACCGTC
    TCACGCACCT CTCGTGTGCT GACGATGCTC TTGGGCATAA TAGCCAAACC CCTGCATCGG
    GACAAGTTCG AAGTAACACC TGACAGTGTG GCCTATTTGA CTGCGCTGGT GGCTGTCTCC
    GAGGAAGTCC GTTCCAGATG TCACGTAAAA CATGCCCTTC CCCGCTGGCC AGCCGATCTG
    AGTAGTAGTG TGGAAAATGG TGAAGCATCC GGCGGAGTGC AAGCTATCGG CCTGCCCCTG
    TCACGCCGTC AAAAAAGTTG GGACATCCTG GATCAGTCCG CCTTGCAGTT TGCCCGCCAA
    CACAAGGTTC CCACTCTTCA GGAACGCGGT TCGCGTTCGT CGGTGTCCAA CGAGTCCAAC
    GTACTGCTCG ATCCCGAGGT CTTACCTGAC CTGTCCATTC AAGCCCTGGT ACTGACTGTT
    TTGGCCACCT TGGTCAAGTA CTCTTCGGAT GAAGGCGAAA CGCGGGTTTT GTATCAGTAT
    TTGGCCGAAG GATCGGTGGT CTTTCCAAAG GTGTTTCCAG TTATCCATTC ACTGCTGGAC
    CAAAAGATAA ACAACATTTT GTCAGTGTCA CACGACCAAG TGGTGCTCAA CTCTGTGCAG
    AACATTATAC AAAATATGCT GGCCAGCGAA GATCCTTCCC AGCAGCAGCT GCACTTCCTG
    CAGAGTTGTG GATTTGGCGG ACTCTGGCGA TTTGCTGGTC CCTTCACCAA GTACAATATG
    ATGGGTGAGT CCTCGGAGCT GTTTGTCAAC TGTTTGGAGG CCATGGTTGA GACCTGTTTG
    CCAGGCGACG AATCCGCGCC GGTGCCACCA TCTCCGCGTC CATACAACCT GAGCTCCAGT
    CTGAGTAGCC TGACCCTGGG ATCACCCACG GATAAAGCTG CATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.