Nf1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nf1
Entrez Gene ID	43149
Full Name	Neurofibromin 1
Synonyms	CG8318,Dmel\CG8318,FBpp0084326,NF-1,NF1,Nf-1,dNF1,dNf1,nf1
General protein information	Preferred Names Neurofibromin 1 Names CG8318 gene product from transcript CG8318-RD CG8318-PB CG8318-PC CG8318-PD CG8318-PE CG8318-PF Neurofibromatosis-1 Nf1-PB Nf1-PC Nf1-PD Nf1-PE Nf1-PF neurofibramatosis-1 neurofibromatosis 1 neurofibromatosis factor 1 neurofibromatosis type 1 neurofibromin neurofibromin 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96F9-96F9

mRNA and Protein(s)

mRNA	Protein	Name
NM_170252.3	NP_733131.2	neurofibromin 1, isoform C
NM_001276049.1	NP_001262978.1	neurofibromin 1, isoform E
NM_170253.2	NP_733132.2	neurofibromin 1, isoform B
NM_001276050.1	NP_001262979.1	neurofibromin 1, isoform F
NM_001014668.2	NP_001014668.1	neurofibromin 1, isoform D

Pathways from BioSystems

Homologies

Homo sapiens (human)	NF1	NP_001035957.1
Rattus norvegicus (Norway rat)	Nf1	NP_036741.1
Danio rerio (zebrafish)	nf1b	XP_692937.4
Drosophila melanogaster (fruit fly)	Nf1	NP_733132.2
	NF1	XP_002800429.1
Canis lupus familiaris (dog)	NF1	XP_537738.3
Mus musculus (house mouse)	Nf1	NP_035027.1
Gallus gallus (chicken)	NF1	XP_004946843.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003140	XP_312829.4
Xenopus tropicalis (tropical clawed frog)	nf1	XP_002936865.2
Pan troglodytes (chimpanzee)	NF1	XP_511395.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Polymorphism in the neurofibromin gene, Nf1, is associated with antagonistic selection on wing size and development time in Drosophila melanogaster.
Title: Polymorphism in the neurofibromin gene, Nf1, is associated with antagonistic selection on wing size and development time in Drosophila melanogaster.

The N-terminal region of NF1 mediates the interaction with Fak56 and is required for the signaling activity and presynaptic localization of NF1
Title: Neurofibromin mediates FAK signaling in confining synapse growth at Drosophila neuromuscular junctions.

These results identify dAlk as an upstream activator of dNf1-regulated Ras signaling responsible for several dNf1 defects, and they implicate human Alk as a potential therapeutic target in NF1
Title: The receptor tyrosine kinase Alk controls neurofibromin functions in Drosophila growth and learning.

memory-related functions of Rut-AC are both Nf1-dependent and -independent, that Nf1 mediates the formation of two distinct memory components within a single neuron population.
Title: A distinct set of Drosophila brain neurons required for neurofibromatosis type 1-dependent learning and memory.

Neurofibromin regulates longevity and stress resistance through cAMP regulation of mitochondrial respiration and ROS production, and NF1 may be treatable using catalytic antioxidants.
Title: Life extension through neurofibromin mitochondrial regulation and antioxidant therapy for neurofibromatosis-1 in Drosophila melanogaster.

The following Nf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06160	NM_001014668.2 Latest version!	Drosophila melanogaster neurofibromin 1, transcript variant D (Nf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa06161	NM_170252.3 Latest version!	Drosophila melanogaster neurofibromin 1, transcript variant C (Nf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa06162	NM_170253.2 Latest version!	Drosophila melanogaster neurofibromin 1, transcript variant B (Nf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa08849	NM_001276049.1 Latest version!	Drosophila melanogaster neurofibromin 1, transcript variant E (Nf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa08850	NM_001276050.1 Latest version!	Drosophila melanogaster neurofibromin 1, transcript variant F (Nf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nf1 ( NM_001014668.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014668.1 Drosophila melanogaster neurofibromin 1, transcript variant D (Nf1), mRNA.

Price & Availability↑

CloneID	OFa06160
Clone ID Related Accession (Same CDS sequence)	NM_001014668.2
Accession Version	NM_001014668.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neurofibromin 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_001014668.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCCAGA AGCCAGGCGA GTGGGCTAGC GCCCTTTTGG CACGTTTCGA GGATCAGCTG 
CCAAACAGAA TCGGTGCCTA TGGCACGCAG GCCAGGATGA GCCAGGACCA ATTGGTGGCC 
TGTCTGATCC ACATATCGCG CTACCGCTTC TCCCTTGTCA TATCCGGTCT CACCAAGATG 
CTGCAGAGGG TCAATGAGGC GGCCCTTCAA AATCGCCATG AGCCAGAGCG TTGCTACTTT 
GAGTCGCTGG TCATCATACT GACCACTCTG GAACGCTGTC TGACCAACCA GACCAAGGAC 
ACAGCACGTT TTGAAGAGGC CATGAATGTG AAGCTTCTGC TGCGCGAGAT CTCCCAGTTT 
GTCGACGTCC AGAGCGACAG CAATCCGAAT GCCGCCCAGC TCAAGGCCCT GGCATCCAAG 
GTGCTCTTTG CCTTGTCCCA GAATCACTTC TCGGCGGTGT TCAATCGCAT ATCGGCCAGG 
ATTCAAGAGC TGACATCCTG TTCCGAGGAA AACCCGGACT ACAACGACAT AGAATTAATT 
CAACACATCG ATATGGATAT GATAAAGCTA ACCAAGCTGC TACAAGAAAC CATCACAAAG 
TTTCGATCGA AGCGTGCTCC ACCTTTGATC TTGCTTTACT CGCTGGAGAA GGCTATTTGG 
AACTGGATCG AGTACCATCC ACAAGAGTTC CAGGACTTGC AACGAGGAAC CAATCGAGAT 
ATATCAACCT GCTGGGAACC CCTGATGGAC TTTGTAGAGT ACTTTAAAAC CGAAAACAAG 
AAAAGCAAGA CTCTTGTTTG GCCGCTTCAA ATGCTCTTGC TGATATTGAA TCCCTCTTGC 
CTTGAGGCTG TTGTTAACGA ACTTCAGCAG TCGGAGAAAG AGAAGGAAAA GGACAAGGAA 
AAGGTTGCCT CGAAGTCAGC GCAATCCACG TCCCGGGACA AGGACTTCTC GGCCAAGCAG 
TTCATCGAGA GTATCAAGAG AGGCTTGGGC CAGCACTCAC CATCCAAACA GGTGACCGAA 
TCTGCGGCAA TTGCCTGTGT AAAGCTGTGC AAAGCATCCA CCTACATCAA CAACACCGAC 
TCCAATAATG TGGTCTTCAA GTTGGTGCAA TTCTTTATCA ACGATCTCAA GGCACTGCTC 
TTTAATCCAG CAAAGCCCTT TTCGCGCGGT CAGGGGTACA ACTTCGCTGA TATCGAGCTG 
ATGATTGACT GCTGGGTGTC TTGTTTCCGG ATTAATCCTC ACAACATCGA GGCTCTAAAG 
GTTTGCCTGA ATTTGTCCTC GCCGCAGGCT TACCATTTTG TAATTGTGTG CTCTCTGCTA 
AGGTTGGCTC ACATATACGT TGACTTCCGT TTGCAGAACA AGAACCCCTT TCGAATAGTA 
AACCAACCGA GATTGTCTTG GTGGCCGCAA ACGGATGTGG TTCACTACCG TTCTGCTGAG 
CTACGAGCTT TGTTTACGGA TACGCTCAAC AAGGCCACCC AGGGTTATAT AGCCCATACG 
CCACTGCGCT ACATAACCTC CCTGACGCTC AAATCTAAAG ATACCCAGAA AGGTTTAACC 
CGCGCTGAAG AGGGTCCCGC CCACAAAATG CTATTACTGC TACTCGTACG GCTAATTCAT 
GCAGATCCCA CGCTTTTGTT GAATACCCAA GGAAAAGTGG CACATGAAGT GCAGAGCTCC 
ACATTGGAGC TCATCAACGG CTTAGTTAGT TTGGTGCATC AAACTACCAT GCCCGATGTG 
GCACAAGAGG CTATGGAGGC GCTGCTTGCT CTGCACGCCC CGGAGAAAAT AGAGGTTTGG 
AATCCAGAGG CTCCGATCAA TACGTTCTGG GACGTTAGCT CCCAAGTACT GTTTTCAATC 
TCGCAAAAGC TCATTCAACA CCAAATTGCC AATTACACTG ATGTCTTAAA GTGGCTACGC 
GAGATCCTCA TCTGTCGGAA TACTTTTCTT CAGCGACACA AGGATTATGC ACATGTGGGA 
AGTCAGATTG CCATTTGCAA GCAGGCTCAC ATTAAGATGG AAGTTGTTTT CTTCATGTAC 
CTGTGGAGTG TTGATTTAGA TGCGGTGCTG ACATCGCTAT CGTGTTTTGG ACTACTATGC 
GAGGAGGCTG AGATTTGCTG CAGTTCTGAT GAATTGACAG TCGGATTTAT TATGCCGAAT 
TACCACATTT ACCAGGAGCT GGCTCAGTTG TCCACATCTG CAACGGACTC TCGCATTTGC 
TTTTTTGACA ACACGCACGG CAATGTGCTG AGCCGTCTTA CACTTCAAAA GCGCATAATG 
ACCCTATTGC GCAAGATCGA GCACTGTGTT CATGGTGTTC AGCCCGCCTG GGAGGAAACC 
TTCCGCAACT GGGAAGTATC CAGCAAGGTT TTGCAAACGT ACCCCAAATG TAAAGGAGAA 
GATGGCCAAG CGGAGGTTTT CCATCGTGGC ATGGGCAAGC GGCGAGCGAG TCACCAAAGC 
TCAGAGCACG ATTTGGAGGA ACAAATTAAT GAATGGGCCA ATATGACATG GTTTCTATTA 
GCCTTGGGTG GCGTCTGCCT TCATAAACGT AGCAGCAGTC GTCAAATGCT GCTGCAGCAA 
TCACAGAACA ATGCTTCTTT GGGATCACTT GCTCAAAACT CTCTTTACTC AAGCTCCACA 
AGTTCTGGAC ATGGCTCTCT GCATCCTAGC ACGGTTTCAC TATCCACTCT TCCCCCAGCA 
CCGCCACAAG ATGTCAGCTA TTGTCCTGTA ACACAATTCG TGGGCCAACT ATTGCGCCTG 
TTAGTTTGCA GCAACGAAAA AATTGGACTT AATATTCAGA AAAATGTGAA GGAACTGGTG 
GGCGAGGAGA TGTCCACCCA ACTGTATCCT ATACTCTTCG ACCAGGTTAG AGCCATCGTG 
GAGAAGTTCT TCGATCAGCA AGGTCAGGTG AACGTCAATG TGACCGACAT CAACACGCAG 
TTTATCGAGC ACACCATCTA CATAATGAAA TCAATTCTGG ATCCCAAAGC CAACAAGGAT 
CCCAACAACG ATCAACCCTC GCCATCGGAA CATTTAGGTG TTACAAGCAT CGAAGGCATG 
ATGCTAGGAA TAGTGCGCTA CGTTCGCCAC CTGGATATGA CTGTTTATGC CATTCGAATC 
AAGACAAAAT TGTGCCAGCT TGTGGAAGTA ATGATGAAGC GACGCGACGA CCTCGCCTTT 
CGCCAGGAGA TGAAGTTCCG GAATAAACTG GTTGAATACT TGACCGACTG GGTGATGGGC 
ACCTCCCATC AGATTGCACC GCCCAGCTCG GCGGATGCCG CTATTCTTAC CAACACATCC 
CTCATATTTC GCGATCTAGA CCAAGCCTGC ATGGAGGCAG TGGCTGCCTT GCTTCGGGGC 
CTTCCCCTTC AGCCTGAGGA ATCGGATCGA GGCGATTTGA TGGATGCAAA GAGTGCGCTC 
TTTTTAAAGT ACTTTACCCT ATTTATGAAC CTGCTGAATG ATTGCATCGA TAGCTCCGAG 
GCGGAGAAGG AAATGAATAA TACCCCACTA TTGCCTCCGC GCCCTCGAAT GGCAGCTGGA 
AAACTGACCG CTCTGCGAAA TGCCACTATT CTAGCCATGT CTAATTTGTT GGGTGCCAAC 
ATTGACTCTG GTTTGATGCA CTCCATCGAT CTGGGTTATA ATCCTGATTT GCAAACGCGT 
GCCGCTTTCA TGGAGGTGTT GACTCAAATC CTGCAACAAG GCACCGAATT CGATACCTTG 
GCCGAAACTG TTTTAGCTGA TCGATTTGAG CAACTGGTCC AACTTGTTAC AATGATCAGC 
GACAAAGGAG AACTTCCCAT AGCAATGGCT TTGGCTAATG TGGTGACTAC ATCCCAAATG 
GACGAGCTGG CTAGGGTTCT GGTCACCCTA TTCGACGCCA AACACCTGTT ATCGCCACTC 
CTATGGAATA TGTTTTATCG CGAGGTGGAG GTCTCAGACT GCATGCAGAC ACTTTTCCGT 
GGAAATTCTT TGGGCAGCAA AATCATGGCC TTTTGCTTTA AAATATACGG CGCGAGCTAT 
CTCCAAATGC TACTAGAGCC TTTAATTCGT CCGCTTCTGG ATGAGGAGGA GGAGACCTGT 
TTTGAGGTGG ATCCAGCTCG TCTGGATCCA ACAGAAGATA TTGAACAGCA CAGGAACAAC 
TTGATTGCCC TAACGCAGAA GGTGTTTGAC GCTATTATCA ACTCGTCGGA TCGCTTTCCC 
CCGCAGCTGC GATCCATGTG CCATTGCCTG TACCAAGTGC TAAGCAAACG CTTCCCGAAT 
CTTCTTCAAA ACAATATCGG TGCTGTAGGC ACAGTCATCT TCTTGCGGTT CATCAATCCC 
GCCATAGTTT CCCCACAGGA ATTGGGAATC GTTGACAAAC AAGTGCACAG CTCGGCCAAA 
CGAGGTCTAA TGCTAATGTC CAAGATCCTT CAAAACATTG CTAACCACGT GGAGTTCTCC 
AAGGAGCAGC ACATGTTGTG TTTCAACGAT TTTCTGCGCG ATCACTTTGA AGCTGGGCGG 
CGATTCTTCA TACAGATTGC ATCAGACTGT GAGACCGTGG ATCAGACCTC GCACAGCATG 
AGTTTTATTT CAGATGCGAA CGTACTGGCA CTGCATCGGT TGCTGTGGAC GCATCAGGAG 
AAGATCGGCG ACTATCTGTC TAGCAGTCGA GACCACAAGG CGGTTGGAAG GCGGCCCTTC 
GACAAGATGG CTACTTTGCT AGCATACCTG GGACCACCGG AGCACAAGCC CGTGGATTCG 
CACATGATGT TCAGCTCGTA TGCACGCTGG AGCTCCATTG ACATGTCGTC GACAAACTTC 
GAGGAGATCA TGGTCAAGCA CCAAATGCAC GAGAAGGAGG AATTCAAGAC CCTCAAGTCG 
ATGAACATAT TCTACCAGGC CGGAACAAGC AAATCTGGCT ATCCTGTCTT TTACTATATA 
GCAAGACGAT ACAAGATTGG AGAAACGAAT GGAGACTTAC TTATATATCA CGTCATACTC 
ACTCTGAAAC CATTCTGCCA CTCGCCCTTC GAGGTGGTCA TCGATTTTAC GCACACCTGT 
TCAGATAATC GGTTCCGCAC CGAGTTTCTG CAGAAGTGGT TTTATGTCCT GCCCACGGTA 
GCCTACGAAA ATGTCCATGC TGTGTACATC TATAACTGTA ACTCGTGGGT GCGCGAATAT 
ACCAAGTTCC ACGATCGCAT TCTGGCACCA TTAAAGGGAA ATCGCAAACT TCTGTTCCTG 
GAGTCACCCA ACAAACTTAC TGATTTCATC GACGCGGAGC AACAGAAATT GCCTGGAGCA 
ACTCTTTCCT TGGACGAAGA TTTAAAGGTA TTCAGCAACG CGCTGAAGCT CAGCCATAAA 
GACACAAAGG TGGCTATCAA AGTGGGTCCC ACCGCATTGC AAATTACATC TGCCGAAAAG 
ACAAAGGTCT TGGCTCACTC CGTGCTCCTG AACGATGTAT ACTACGCCTC TGAAATTGAG 
GAGGTGTGCT TGGTGGACGA TAACCAGTTC ACCCTGTCTA TAACTAACGA AAGTGGCCAG 
CTTAGCTTTA TTCACAACGA TTGCGACAAC ATTGTTCAAG CCATCATACA CATCCGAAAT 
CGATGGGAGC TTAGTCAGCC GGACTCCGTG ACGGTTCACC AAAAGATCCG ACCAAAAGAT 
GTGCCTGGAA CGCTGCTGAA CATGGCGCTA CTCAATCTGG GATCATGTGA CCCCAATCTA 
AGGACTGCTG CCTACAATTT GCTGTGTGCT TTGACCGCTA CTTTTGATCT AAAGATTGAG 
GGTCAACTGT TGGAGACCCA AGGACTTTGC ATACCCTCAA ATAATACCAT ATTTATCAAA 
TCCGTTAGCG AAAAGCTGGC CACCAATGAA CCGCATCTGA CTCTGGAATT CCTTGAGGAG 
TCCATTCAGG GCTTCCAGCG CAGCACCATA GAGCTGAAAC ATTTGTGTTT GGAGTACATG 
ACGCCATGGT TAAAGAACCT AGTCAAATTT TGCAAGTCCA ACGATGACTC CAAAAAGCTT 
AAGGTCTCCC AAATTCTGGA CAAGCTCATC AATTTAACTA TTGACCAAAA GGAAATGTAT 
CCCTCAGTGC AGGCCAAGAT CTGGGGATCA ATTGGACAGA TCCCCGAGCT GATCGACATG 
GTTTTGGATA ACTTTTTGCA CAAATCGATC ACATATGGCT TGGGATCACC ACAGGTGGAG 
ATTATGGCTG ACACGGCAGT GGCTCTCGCT TCAGCGAATG TTCAGCTGGT GTCCAAAAAG 
GTCATAACGC GAATATGCCG AGTCATGGAC AAATCCTGCA CAAATCCCAC ACAATATCTG 
GAGCAGCATA TGATGTGGGA CGATATTGCA ATTCTTGGTC GATACCTGCT CATGTTGTCG 
TTCAACAATT GTTTGGATGT GGCCACATCG GTGCCATATC TATTCCACAC CATTACGTTT 
TTGGTATGTT CAGGATCCCT GTCGATGCGA GCCTCTACTC ATGGCCTAGT GATCAACATC 
ATCCACTCGC TGTGCACATG CACAAATCCC TCATTTTCAG AGGAGGCGCA GCGAGTACTC 
CGACTGTCCC TGGATGAATT CTCACTGCCC AAGTTCTATC TACTCTTCGG CATCAGCAAG 
GTCAAGTCCG CAGCAGTTAC CGCTTTCCGC TCCAGCTGCC GTCACCCTAC AGATAAATGG 
CTAGGAAATG AAAGGGTTAC CCAGCCTCTG CCCGCCGATC GAGAGCGTTT ATCCCTACCA 
TCGCTTGAGG TTATCACGGA TGCCCTGCTG GAAATCATGG AGGCTTGCAT GCGAGATGTT 
CCGGACTGCG AATGGCTCAA CACCTGGACC TCTTTGGCTC GCAGTTTTGC TTTCTGTTAC 
AATCCAGCGC TACAGCCTAG AGCCCTTATA GTTTACGGGT GCATCAGCAA GAGTGTTACC 
GATCACGAGG TCAAGCAGTT ACTACGCATC CTGGTTAAGG CCCTCGAATC TTTCAACGAT 
CTCATTCTGA TCGAGGCTCT AGTAATGTGC TTAACTCGCA TTCAACCACT ACTCCGACCA 
GAGTCGCCCA TTCATCGGGC CCTCTTCTGG GTGGCCATTT CGGTGCTGCA GTTGGACGAG 
ATTACCCTGT ACGGCGCTGG ACTAGCTCTG CTTGAACAAA ATCTACACAC GCTTAAGTCA 
CAGGGCTGTT TTGACAAGAA GGAGACCATA GCCGAGGTCA TGATGAAGAC AAGGGAAAAG 
CTGGAGTGGC ATTTCAAGCA ACTGGATCAC GCCGTCGGCC TCTCCTTCCG CAGCAATTTC 
CACTTTGCCC TAGTGGGACA CTTGATTAAG GGCTTCCGCC ATCCTACACC TACAACCGTC 
TCACGCACCT CTCGTGTGCT GACGATGCTC TTGGGCATAA TAGCCAAACC CCTGCATCGG 
GACAAGTTCG AAGTAACACC TGACAGTGTG GCCTATTTGA CTGCGCTGGT GGCTGTCTCC 
GAGGAAGTCC GTTCCAGATG TCACGTAAAA CATGCCCTTC CCCGCTGGCC AGCCGATCTG 
AGTAGTAGTG TGGAAAATGG TGAAGCATCC GGCGGAGTGC AAGCTATCGG CCTGCCCCTG 
TCACGCCGTC AAAAAAGTTG GGACATCCTG GATCAGTCCG CCTTGCAGTT TGCCCGCCAA 
CACAAGGTTC CCACTCTTCA GAACGCGCGA GTTTTGTTCA AAACTCAACG CTCATTCTCG 
GTGCCGACGA CCAAAGATCC GAACAATGCC ACCGGCATCG AAGAACGTCA GGAACGCGGT 
TCGCGTTCGT CGGTGTCCAA CGAGTCCAAC GTACTGCTCG ATCCCGAGGT CTTACCTGAC 
CTGTCCATTC AAGCCCTGGT ACTGACTGTT TTGGCCACCT TGGTCAAGTA CTCTTCGGAT 
GAAGGCGAAA CGCGGGTTTT GTATCAGTAT TTGGCCGAAG GATCGGTGGT CTTTCCAAAG 
GTGTTTCCAG TTATCCATTC ACTGCTGGAC CAAAAGATAA ACAACATTTT GTCAGTGTCA 
CACGACCAAG TGGTGCTCAA CTCTGTGCAG AACATTATAC AAAATATGCT GGCCAGCGAA 
GATCCTTCCC AGCAGCAGCT GCACTTCCTG CAGAGTTGTG GATTTGGCGG ACTCTGGCGA 
TTTGCTGGTC CCTTCACCAA GTACAATATG ATGGGTGAGT CCTCGGAGCT GTTTGTCAAC 
TGTTTGGAGG CCATGGTTGA GACCTGTTTG CCAGGCGACG AATCCGCGCC GGTGCCACCA 
TCTCCGCGTC CATACAACCT GAGCTCCAGT CTGAGTAGCC TGACCCTGGG ATCACCCACG 
GATAAAGCTG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014668.1
CDS	280..8574
Translation	MTQKPGEWASALLARFEDQLPNRIGAYGTQARMSQDQLVACLIHISRYRFSLVISGLTKMLQRVNEAALQNRHEPERCYFESLVIILTTLERCLTNQTKDTARFEEAMNVKLLLREISQFVDVQSDSNPNAAQLKALASKVLFALSQNHFSAVFNRISARIQELTSCSEENPDYNDIELIQHIDMDMIKLTKLLQETITKFRSKRAPPLILLYSLEKAIWNWIEYHPQEFQDLQRGTNRDISTCWEPLMDFVEYFKTENKKSKTLVWPLQMLLLILNPSCLEAVVNELQQSEKEKEKDKEKVASKSAQSTSRDKDFSAKQFIESIKRGLGQHSPSKQVTESAAIACVKLCKASTYINNTDSNNVVFKLVQFFINDLKALLFNPAKPFSRGQGYNFADIELMIDCWVSCFRINPHNIEALKVCLNLSSPQAYHFVIVCSLLRLAHIYVDFRLQNKNPFRIVNQPRLSWWPQTDVVHYRSAELRALFTDTLNKATQGYIAHTPLRYITSLTLKSKDTQKGLTRAEEGPAHKMLLLLLVRLIHADPTLLLNTQGKVAHEVQSSTLELINGLVSLVHQTTMPDVAQEAMEALLALHAPEKIEVWNPEAPINTFWDVSSQVLFSISQKLIQHQIANYTDVLKWLREILICRNTFLQRHKDYAHVGSQIAICKQAHIKMEVVFFMYLWSVDLDAVLTSLSCFGLLCEEAEICCSSDELTVGFIMPNYHIYQELAQLSTSATDSRICFFDNTHGNVLSRLTLQKRIMTLLRKIEHCVHGVQPAWEETFRNWEVSSKVLQTYPKCKGEDGQAEVFHRGMGKRRASHQSSEHDLEEQINEWANMTWFLLALGGVCLHKRSSSRQMLLQQSQNNASLGSLAQNSLYSSSTSSGHGSLHPSTVSLSTLPPAPPQDVSYCPVTQFVGQLLRLLVCSNEKIGLNIQKNVKELVGEEMSTQLYPILFDQVRAIVEKFFDQQGQVNVNVTDINTQFIEHTIYIMKSILDPKANKDPNNDQPSPSEHLGVTSIEGMMLGIVRYVRHLDMTVYAIRIKTKLCQLVEVMMKRRDDLAFRQEMKFRNKLVEYLTDWVMGTSHQIAPPSSADAAILTNTSLIFRDLDQACMEAVAALLRGLPLQPEESDRGDLMDAKSALFLKYFTLFMNLLNDCIDSSEAEKEMNNTPLLPPRPRMAAGKLTALRNATILAMSNLLGANIDSGLMHSIDLGYNPDLQTRAAFMEVLTQILQQGTEFDTLAETVLADRFEQLVQLVTMISDKGELPIAMALANVVTTSQMDELARVLVTLFDAKHLLSPLLWNMFYREVEVSDCMQTLFRGNSLGSKIMAFCFKIYGASYLQMLLEPLIRPLLDEEEETCFEVDPARLDPTEDIEQHRNNLIALTQKVFDAIINSSDRFPPQLRSMCHCLYQVLSKRFPNLLQNNIGAVGTVIFLRFINPAIVSPQELGIVDKQVHSSAKRGLMLMSKILQNIANHVEFSKEQHMLCFNDFLRDHFEAGRRFFIQIASDCETVDQTSHSMSFISDANVLALHRLLWTHQEKIGDYLSSSRDHKAVGRRPFDKMATLLAYLGPPEHKPVDSHMMFSSYARWSSIDMSSTNFEEIMVKHQMHEKEEFKTLKSMNIFYQAGTSKSGYPVFYYIARRYKIGETNGDLLIYHVILTLKPFCHSPFEVVIDFTHTCSDNRFRTEFLQKWFYVLPTVAYENVHAVYIYNCNSWVREYTKFHDRILAPLKGNRKLLFLESPNKLTDFIDAEQQKLPGATLSLDEDLKVFSNALKLSHKDTKVAIKVGPTALQITSAEKTKVLAHSVLLNDVYYASEIEEVCLVDDNQFTLSITNESGQLSFIHNDCDNIVQAIIHIRNRWELSQPDSVTVHQKIRPKDVPGTLLNMALLNLGSCDPNLRTAAYNLLCALTATFDLKIEGQLLETQGLCIPSNNTIFIKSVSEKLATNEPHLTLEFLEESIQGFQRSTIELKHLCLEYMTPWLKNLVKFCKSNDDSKKLKVSQILDKLINLTIDQKEMYPSVQAKIWGSIGQIPELIDMVLDNFLHKSITYGLGSPQVEIMADTAVALASANVQLVSKKVITRICRVMDKSCTNPTQYLEQHMMWDDIAILGRYLLMLSFNNCLDVATSVPYLFHTITFLVCSGSLSMRASTHGLVINIIHSLCTCTNPSFSEEAQRVLRLSLDEFSLPKFYLLFGISKVKSAAVTAFRSSCRHPTDKWLGNERVTQPLPADRERLSLPSLEVITDALLEIMEACMRDVPDCEWLNTWTSLARSFAFCYNPALQPRALIVYGCISKSVTDHEVKQLLRILVKALESFNDLILIEALVMCLTRIQPLLRPESPIHRALFWVAISVLQLDEITLYGAGLALLEQNLHTLKSQGCFDKKETIAEVMMKTREKLEWHFKQLDHAVGLSFRSNFHFALVGHLIKGFRHPTPTTVSRTSRVLTMLLGIIAKPLHRDKFEVTPDSVAYLTALVAVSEEVRSRCHVKHALPRWPADLSSSVENGEASGGVQAIGLPLSRRQKSWDILDQSALQFARQHKVPTLQNARVLFKTQRSFSVPTTKDPNNATGIEERQERGSRSSVSNESNVLLDPEVLPDLSIQALVLTVLATLVKYSSDEGETRVLYQYLAEGSVVFPKVFPVIHSLLDQKINNILSVSHDQVVLNSVQNIIQNMLASEDPSQQQLHFLQSCGFGGLWRFAGPFTKYNMMGESSELFVNCLEAMVETCLPGDESAPVPPSPRPYNLSSSLSSLTLGSPTDKAA

Target ORF information:

RefSeq Version	NM_001014668.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neurofibromin 1, transcript variant D (Nf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014668.2

ORF Insert Sequence:

ATGACCCAGA AGCCAGGCGA GTGGGCTAGC GCCCTTTTGG CACGTTTCGA GGATCAGCTG 
CCAAACAGAA TCGGTGCCTA TGGCACGCAG GCCAGGATGA GCCAGGACCA ATTGGTGGCC 
TGTCTGATCC ACATATCGCG CTACCGCTTC TCCCTTGTCA TATCCGGTCT CACCAAGATG 
CTGCAGAGGG TCAATGAGGC GGCCCTTCAA AATCGCCATG AGCCAGAGCG TTGCTACTTT 
GAGTCGCTGG TCATCATACT GACCACTCTG GAACGCTGTC TGACCAACCA GACCAAGGAC 
ACAGCACGTT TTGAAGAGGC CATGAATGTG AAGCTTCTGC TGCGCGAGAT CTCCCAGTTT 
GTCGACGTCC AGAGCGACAG CAATCCGAAT GCCGCCCAGC TCAAGGCCCT GGCATCCAAG 
GTGCTCTTTG CCTTGTCCCA GAATCACTTC TCGGCGGTGT TCAATCGCAT ATCGGCCAGG 
ATTCAAGAGC TGACATCCTG TTCCGAGGAA AACCCGGACT ACAACGACAT AGAATTAATT 
CAACACATCG ATATGGATAT GATAAAGCTA ACCAAGCTGC TACAAGAAAC CATCACAAAG 
TTTCGATCGA AGCGTGCTCC ACCTTTGATC TTGCTTTACT CGCTGGAGAA GGCTATTTGG 
AACTGGATCG AGTACCATCC ACAAGAGTTC CAGGACTTGC AACGAGGAAC CAATCGAGAT 
ATATCAACCT GCTGGGAACC CCTGATGGAC TTTGTAGAGT ACTTTAAAAC CGAAAACAAG 
AAAAGCAAGA CTCTTGTTTG GCCGCTTCAA ATGCTCTTGC TGATATTGAA TCCCTCTTGC 
CTTGAGGCTG TTGTTAACGA ACTTCAGCAG TCGGAGAAAG AGAAGGAAAA GGACAAGGAA 
AAGGTTGCCT CGAAGTCAGC GCAATCCACG TCCCGGGACA AGGACTTCTC GGCCAAGCAG 
TTCATCGAGA GTATCAAGAG AGGCTTGGGC CAGCACTCAC CATCCAAACA GGTGACCGAA 
TCTGCGGCAA TTGCCTGTGT AAAGCTGTGC AAAGCATCCA CCTACATCAA CAACACCGAC 
TCCAATAATG TGGTCTTCAA GTTGGTGCAA TTCTTTATCA ACGATCTCAA GGCACTGCTC 
TTTAATCCAG CAAAGCCCTT TTCGCGCGGT CAGGGGTACA ACTTCGCTGA TATCGAGCTG 
ATGATTGACT GCTGGGTGTC TTGTTTCCGG ATTAATCCTC ACAACATCGA GGCTCTAAAG 
GTTTGCCTGA ATTTGTCCTC GCCGCAGGCT TACCATTTTG TAATTGTGTG CTCTCTGCTA 
AGGTTGGCTC ACATATACGT TGACTTCCGT TTGCAGAACA AGAACCCCTT TCGAATAGTA 
AACCAACCGA GATTGTCTTG GTGGCCGCAA ACGGATGTGG TTCACTACCG TTCTGCTGAG 
CTACGAGCTT TGTTTACGGA TACGCTCAAC AAGGCCACCC AGGGTTATAT AGCCCATACG 
CCACTGCGCT ACATAACCTC CCTGACGCTC AAATCTAAAG ATACCCAGAA AGGTTTAACC 
CGCGCTGAAG AGGGTCCCGC CCACAAAATG CTATTACTGC TACTCGTACG GCTAATTCAT 
GCAGATCCCA CGCTTTTGTT GAATACCCAA GGAAAAGTGG CACATGAAGT GCAGAGCTCC 
ACATTGGAGC TCATCAACGG CTTAGTTAGT TTGGTGCATC AAACTACCAT GCCCGATGTG 
GCACAAGAGG CTATGGAGGC GCTGCTTGCT CTGCACGCCC CGGAGAAAAT AGAGGTTTGG 
AATCCAGAGG CTCCGATCAA TACGTTCTGG GACGTTAGCT CCCAAGTACT GTTTTCAATC 
TCGCAAAAGC TCATTCAACA CCAAATTGCC AATTACACTG ATGTCTTAAA GTGGCTACGC 
GAGATCCTCA TCTGTCGGAA TACTTTTCTT CAGCGACACA AGGATTATGC ACATGTGGGA 
AGTCAGATTG CCATTTGCAA GCAGGCTCAC ATTAAGATGG AAGTTGTTTT CTTCATGTAC 
CTGTGGAGTG TTGATTTAGA TGCGGTGCTG ACATCGCTAT CGTGTTTTGG ACTACTATGC 
GAGGAGGCTG AGATTTGCTG CAGTTCTGAT GAATTGACAG TCGGATTTAT TATGCCGAAT 
TACCACATTT ACCAGGAGCT GGCTCAGTTG TCCACATCTG CAACGGACTC TCGCATTTGC 
TTTTTTGACA ACACGCACGG CAATGTGCTG AGCCGTCTTA CACTTCAAAA GCGCATAATG 
ACCCTATTGC GCAAGATCGA GCACTGTGTT CATGGTGTTC AGCCCGCCTG GGAGGAAACC 
TTCCGCAACT GGGAAGTATC CAGCAAGGTT TTGCAAACGT ACCCCAAATG TAAAGGAGAA 
GATGGCCAAG CGGAGGTTTT CCATCGTGGC ATGGGCAAGC GGCGAGCGAG TCACCAAAGC 
TCAGAGCACG ATTTGGAGGA ACAAATTAAT GAATGGGCCA ATATGACATG GTTTCTATTA 
GCCTTGGGTG GCGTCTGCCT TCATAAACGT AGCAGCAGTC GTCAAATGCT GCTGCAGCAA 
TCACAGAACA ATGCTTCTTT GGGATCACTT GCTCAAAACT CTCTTTACTC AAGCTCCACA 
AGTTCTGGAC ATGGCTCTCT GCATCCTAGC ACGGTTTCAC TATCCACTCT TCCCCCAGCA 
CCGCCACAAG ATGTCAGCTA TTGTCCTGTA ACACAATTCG TGGGCCAACT ATTGCGCCTG 
TTAGTTTGCA GCAACGAAAA AATTGGACTT AATATTCAGA AAAATGTGAA GGAACTGGTG 
GGCGAGGAGA TGTCCACCCA ACTGTATCCT ATACTCTTCG ACCAGGTTAG AGCCATCGTG 
GAGAAGTTCT TCGATCAGCA AGGTCAGGTG AACGTCAATG TGACCGACAT CAACACGCAG 
TTTATCGAGC ACACCATCTA CATAATGAAA TCAATTCTGG ATCCCAAAGC CAACAAGGAT 
CCCAACAACG ATCAACCCTC GCCATCGGAA CATTTAGGTG TTACAAGCAT CGAAGGCATG 
ATGCTAGGAA TAGTGCGCTA CGTTCGCCAC CTGGATATGA CTGTTTATGC CATTCGAATC 
AAGACAAAAT TGTGCCAGCT TGTGGAAGTA ATGATGAAGC GACGCGACGA CCTCGCCTTT 
CGCCAGGAGA TGAAGTTCCG GAATAAACTG GTTGAATACT TGACCGACTG GGTGATGGGC 
ACCTCCCATC AGATTGCACC GCCCAGCTCG GCGGATGCCG CTATTCTTAC CAACACATCC 
CTCATATTTC GCGATCTAGA CCAAGCCTGC ATGGAGGCAG TGGCTGCCTT GCTTCGGGGC 
CTTCCCCTTC AGCCTGAGGA ATCGGATCGA GGCGATTTGA TGGATGCAAA GAGTGCGCTC 
TTTTTAAAGT ACTTTACCCT ATTTATGAAC CTGCTGAATG ATTGCATCGA TAGCTCCGAG 
GCGGAGAAGG AAATGAATAA TACCCCACTA TTGCCTCCGC GCCCTCGAAT GGCAGCTGGA 
AAACTGACCG CTCTGCGAAA TGCCACTATT CTAGCCATGT CTAATTTGTT GGGTGCCAAC 
ATTGACTCTG GTTTGATGCA CTCCATCGAT CTGGGTTATA ATCCTGATTT GCAAACGCGT 
GCCGCTTTCA TGGAGGTGTT GACTCAAATC CTGCAACAAG GCACCGAATT CGATACCTTG 
GCCGAAACTG TTTTAGCTGA TCGATTTGAG CAACTGGTCC AACTTGTTAC AATGATCAGC 
GACAAAGGAG AACTTCCCAT AGCAATGGCT TTGGCTAATG TGGTGACTAC ATCCCAAATG 
GACGAGCTGG CTAGGGTTCT GGTCACCCTA TTCGACGCCA AACACCTGTT ATCGCCACTC 
CTATGGAATA TGTTTTATCG CGAGGTGGAG GTCTCAGACT GCATGCAGAC ACTTTTCCGT 
GGAAATTCTT TGGGCAGCAA AATCATGGCC TTTTGCTTTA AAATATACGG CGCGAGCTAT 
CTCCAAATGC TACTAGAGCC TTTAATTCGT CCGCTTCTGG ATGAGGAGGA GGAGACCTGT 
TTTGAGGTGG ATCCAGCTCG TCTGGATCCA ACAGAAGATA TTGAACAGCA CAGGAACAAC 
TTGATTGCCC TAACGCAGAA GGTGTTTGAC GCTATTATCA ACTCGTCGGA TCGCTTTCCC 
CCGCAGCTGC GATCCATGTG CCATTGCCTG TACCAAGTGC TAAGCAAACG CTTCCCGAAT 
CTTCTTCAAA ACAATATCGG TGCTGTAGGC ACAGTCATCT TCTTGCGGTT CATCAATCCC 
GCCATAGTTT CCCCACAGGA ATTGGGAATC GTTGACAAAC AAGTGCACAG CTCGGCCAAA 
CGAGGTCTAA TGCTAATGTC CAAGATCCTT CAAAACATTG CTAACCACGT GGAGTTCTCC 
AAGGAGCAGC ACATGTTGTG TTTCAACGAT TTTCTGCGCG ATCACTTTGA AGCTGGGCGG 
CGATTCTTCA TACAGATTGC ATCAGACTGT GAGACCGTGG ATCAGACCTC GCACAGCATG 
AGTTTTATTT CAGATGCGAA CGTACTGGCA CTGCATCGGT TGCTGTGGAC GCATCAGGAG 
AAGATCGGCG ACTATCTGTC TAGCAGTCGA GACCACAAGG CGGTTGGAAG GCGGCCCTTC 
GACAAGATGG CTACTTTGCT AGCATACCTG GGACCACCGG AGCACAAGCC CGTGGATTCG 
CACATGATGT TCAGCTCGTA TGCACGCTGG AGCTCCATTG ACATGTCGTC GACAAACTTC 
GAGGAGATCA TGGTCAAGCA CCAAATGCAC GAGAAGGAGG AATTCAAGAC CCTCAAGTCG 
ATGAACATAT TCTACCAGGC CGGAACAAGC AAATCTGGCT ATCCTGTCTT TTACTATATA 
GCAAGACGAT ACAAGATTGG AGAAACGAAT GGAGACTTAC TTATATATCA CGTCATACTC 
ACTCTGAAAC CATTCTGCCA CTCGCCCTTC GAGGTGGTCA TCGATTTTAC GCACACCTGT 
TCAGATAATC GGTTCCGCAC CGAGTTTCTG CAGAAGTGGT TTTATGTCCT GCCCACGGTA 
GCCTACGAAA ATGTCCATGC TGTGTACATC TATAACTGTA ACTCGTGGGT GCGCGAATAT 
ACCAAGTTCC ACGATCGCAT TCTGGCACCA TTAAAGGGAA ATCGCAAACT TCTGTTCCTG 
GAGTCACCCA ACAAACTTAC TGATTTCATC GACGCGGAGC AACAGAAATT GCCTGGAGCA 
ACTCTTTCCT TGGACGAAGA TTTAAAGGTA TTCAGCAACG CGCTGAAGCT CAGCCATAAA 
GACACAAAGG TGGCTATCAA AGTGGGTCCC ACCGCATTGC AAATTACATC TGCCGAAAAG 
ACAAAGGTCT TGGCTCACTC CGTGCTCCTG AACGATGTAT ACTACGCCTC TGAAATTGAG 
GAGGTGTGCT TGGTGGACGA TAACCAGTTC ACCCTGTCTA TAACTAACGA AAGTGGCCAG 
CTTAGCTTTA TTCACAACGA TTGCGACAAC ATTGTTCAAG CCATCATACA CATCCGAAAT 
CGATGGGAGC TTAGTCAGCC GGACTCCGTG ACGGTTCACC AAAAGATCCG ACCAAAAGAT 
GTGCCTGGAA CGCTGCTGAA CATGGCGCTA CTCAATCTGG GATCATGTGA CCCCAATCTA 
AGGACTGCTG CCTACAATTT GCTGTGTGCT TTGACCGCTA CTTTTGATCT AAAGATTGAG 
GGTCAACTGT TGGAGACCCA AGGACTTTGC ATACCCTCAA ATAATACCAT ATTTATCAAA 
TCCGTTAGCG AAAAGCTGGC CACCAATGAA CCGCATCTGA CTCTGGAATT CCTTGAGGAG 
TCCATTCAGG GCTTCCAGCG CAGCACCATA GAGCTGAAAC ATTTGTGTTT GGAGTACATG 
ACGCCATGGT TAAAGAACCT AGTCAAATTT TGCAAGTCCA ACGATGACTC CAAAAAGCTT 
AAGGTCTCCC AAATTCTGGA CAAGCTCATC AATTTAACTA TTGACCAAAA GGAAATGTAT 
CCCTCAGTGC AGGCCAAGAT CTGGGGATCA ATTGGACAGA TCCCCGAGCT GATCGACATG 
GTTTTGGATA ACTTTTTGCA CAAATCGATC ACATATGGCT TGGGATCACC ACAGGTGGAG 
ATTATGGCTG ACACGGCAGT GGCTCTCGCT TCAGCGAATG TTCAGCTGGT GTCCAAAAAG 
GTCATAACGC GAATATGCCG AGTCATGGAC AAATCCTGCA CAAATCCCAC ACAATATCTG 
GAGCAGCATA TGATGTGGGA CGATATTGCA ATTCTTGGTC GATACCTGCT CATGTTGTCG 
TTCAACAATT GTTTGGATGT GGCCACATCG GTGCCATATC TATTCCACAC CATTACGTTT 
TTGGTATGTT CAGGATCCCT GTCGATGCGA GCCTCTACTC ATGGCCTAGT GATCAACATC 
ATCCACTCGC TGTGCACATG CACAAATCCC TCATTTTCAG AGGAGGCGCA GCGAGTACTC 
CGACTGTCCC TGGATGAATT CTCACTGCCC AAGTTCTATC TACTCTTCGG CATCAGCAAG 
GTCAAGTCCG CAGCAGTTAC CGCTTTCCGC TCCAGCTGCC GTCACCCTAC AGATAAATGG 
CTAGGAAATG AAAGGGTTAC CCAGCCTCTG CCCGCCGATC GAGAGCGTTT ATCCCTACCA 
TCGCTTGAGG TTATCACGGA TGCCCTGCTG GAAATCATGG AGGCTTGCAT GCGAGATGTT 
CCGGACTGCG AATGGCTCAA CACCTGGACC TCTTTGGCTC GCAGTTTTGC TTTCTGTTAC 
AATCCAGCGC TACAGCCTAG AGCCCTTATA GTTTACGGGT GCATCAGCAA GAGTGTTACC 
GATCACGAGG TCAAGCAGTT ACTACGCATC CTGGTTAAGG CCCTCGAATC TTTCAACGAT 
CTCATTCTGA TCGAGGCTCT AGTAATGTGC TTAACTCGCA TTCAACCACT ACTCCGACCA 
GAGTCGCCCA TTCATCGGGC CCTCTTCTGG GTGGCCATTT CGGTGCTGCA GTTGGACGAG 
ATTACCCTGT ACGGCGCTGG ACTAGCTCTG CTTGAACAAA ATCTACACAC GCTTAAGTCA 
CAGGGCTGTT TTGACAAGAA GGAGACCATA GCCGAGGTCA TGATGAAGAC AAGGGAAAAG 
CTGGAGTGGC ATTTCAAGCA ACTGGATCAC GCCGTCGGCC TCTCCTTCCG CAGCAATTTC 
CACTTTGCCC TAGTGGGACA CTTGATTAAG GGCTTCCGCC ATCCTACACC TACAACCGTC 
TCACGCACCT CTCGTGTGCT GACGATGCTC TTGGGCATAA TAGCCAAACC CCTGCATCGG 
GACAAGTTCG AAGTAACACC TGACAGTGTG GCCTATTTGA CTGCGCTGGT GGCTGTCTCC 
GAGGAAGTCC GTTCCAGATG TCACGTAAAA CATGCCCTTC CCCGCTGGCC AGCCGATCTG 
AGTAGTAGTG TGGAAAATGG TGAAGCATCC GGCGGAGTGC AAGCTATCGG CCTGCCCCTG 
TCACGCCGTC AAAAAAGTTG GGACATCCTG GATCAGTCCG CCTTGCAGTT TGCCCGCCAA 
CACAAGGTTC CCACTCTTCA GAACGCGCGA GTTTTGTTCA AAACTCAACG CTCATTCTCG 
GTGCCGACGA CCAAAGATCC GAACAATGCC ACCGGCATCG AAGAACGTCA GGAACGCGGT 
TCGCGTTCGT CGGTGTCCAA CGAGTCCAAC GTACTGCTCG ATCCCGAGGT CTTACCTGAC 
CTGTCCATTC AAGCCCTGGT ACTGACTGTT TTGGCCACCT TGGTCAAGTA CTCTTCGGAT 
GAAGGCGAAA CGCGGGTTTT GTATCAGTAT TTGGCCGAAG GATCGGTGGT CTTTCCAAAG 
GTGTTTCCAG TTATCCATTC ACTGCTGGAC CAAAAGATAA ACAACATTTT GTCAGTGTCA 
CACGACCAAG TGGTGCTCAA CTCTGTGCAG AACATTATAC AAAATATGCT GGCCAGCGAA 
GATCCTTCCC AGCAGCAGCT GCACTTCCTG CAGAGTTGTG GATTTGGCGG ACTCTGGCGA 
TTTGCTGGTC CCTTCACCAA GTACAATATG ATGGGTGAGT CCTCGGAGCT GTTTGTCAAC 
TGTTTGGAGG CCATGGTTGA GACCTGTTTG CCAGGCGACG AATCCGCGCC GGTGCCACCA 
TCTCCGCGTC CATACAACCT GAGCTCCAGT CTGAGTAGCC TGACCCTGGG ATCACCCACG 
GATAAAGCTG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nf1 ( NM_170252.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733131.2 Drosophila melanogaster neurofibromin 1, transcript variant C (Nf1), mRNA.

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CloneID	OFa06161
Clone ID Related Accession (Same CDS sequence)	NM_170252.3
Accession Version	NM_170252.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neurofibromin 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_170252.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCCAGA AGCCAGGCGA GTGGGCTAGC GCCCTTTTGG CACGTTTCGA GGATCAGCTG 
CCAAACAGAA TCGGTGCCTA TGGCACGCAG GCCAGGATGA GCCAGGACCA ATTGGTGGCC 
TGTCTGATCC ACATATCGCG CTACCGCTTC TCCCTTGTCA TATCCGGTCT CACCAAGATG 
CTGCAGAGGG TCAATGAGGC GGCCCTTCAA AATCGCCATG AGCCAGAGCG TTGCTACTTT 
GAGTCGCTGG TCATCATACT GACCACTCTG GAACGCTGTC TGACCAACCA GACCAAGGAC 
ACAGCACGTT TTGAAGAGGC CATGAATGTG AAGCTTCTGC TGCGCGAGAT CTCCCAGTTT 
GTCGACGTCC AGAGCGACAG CAATCCGAAT GCCGCCCAGC TCAAGGCCCT GGCATCCAAG 
GTGCTCTTTG CCTTGTCCCA GAATCACTTC TCGGCGGTGT TCAATCGCAT ATCGGCCAGG 
ATTCAAGAGC TGACATCCTG TTCCGAGGAA AACCCGGACT ACAACGACAT AGAATTAATT 
CAACACATCG ATATGGATAT GATAAAGCTA ACCAAGCTGC TACAAGAAAC CATCACAAAG 
TTTCGATCGA AGCGTGCTCC ACCTTTGATC TTGCTTTACT CGCTGGAGAA GGCTATTTGG 
AACTGGATCG AGTACCATCC ACAAGAGTTC CAGGACTTGC AACGAGGAAC CAATCGAGAT 
ATATCAACCT GCTGGGAACC CCTGATGGAC TTTGTAGAGT ACTTTAAAAC CGAAAACAAG 
AAAAGCAAGA CTCTTGTTTG GCCGCTTCAA ATGCTCTTGC TGATATTGAA TCCCTCTTGC 
CTTGAGGCTG TTGTTAACGA ACTTCAGCAG TCGGAGAAAG AGAAGGAAAA GGACAAGGAA 
AAGGTTGCCT CGAAGTCAGC GCAATCCACG TCCCGGGACA AGGACTTCTC GGCCAAGCAG 
TTCATCGAGA GTATCAAGAG AGGCTTGGGC CAGCACTCAC CATCCAAACA GGTGACCGAA 
TCTGCGGCAA TTGCCTGTGT AAAGCTGTGC AAAGCATCCA CCTACATCAA CAACACCGAC 
TCCAATAATG TGGTCTTCAA GTTGGTGCAA TTCTTTATCA ACGATCTCAA GGCACTGCTC 
TTTAATCCAG CAAAGCCCTT TTCGCGCGGT CAGGGGTACA ACTTCGCTGA TATCGAGCTG 
ATGATTGACT GCTGGGTGTC TTGTTTCCGG ATTAATCCTC ACAACATCGA GGCTCTAAAG 
GTTTGCCTGA ATTTGTCCTC GCCGCAGGCT TACCATTTTG TAATTGTGTG CTCTCTGCTA 
AGGTTGGCTC ACATATACGT TGACTTCCGT TTGCAGAACA AGAACCCCTT TCGAATAGTA 
AACCAACCGA GATTGTCTTG GTGGCCGCAA ACGGATGTGG TTCACTACCG TTCTGCTGAG 
CTACGAGCTT TGTTTACGGA TACGCTCAAC AAGGCCACCC AGGGTTATAT AGCCCATACG 
CCACTGCGCT ACATAACCTC CCTGACGCTC AAATCTAAAG ATACCCAGAA AGGTTTAACC 
CGCGCTGAAG AGGGTCCCGC CCACAAAATG CTATTACTGC TACTCGTACG GCTAATTCAT 
GCAGATCCCA CGCTTTTGTT GAATACCCAA GGAAAAGTGG CACATGAAGT GCAGAGCTCC 
ACATTGGAGC TCATCAACGG CTTAGTTAGT TTGGTGCATC AAACTACCAT GCCCGATGTG 
GCACAAGAGG CTATGGAGGC GCTGCTTGCT CTGCACGCCC CGGAGAAAAT AGAGGTTTGG 
AATCCAGAGG CTCCGATCAA TACGTTCTGG GACGTTAGCT CCCAAGTACT GTTTTCAATC 
TCGCAAAAGC TCATTCAACA CCAAATTGCC AATTACACTG ATGTCTTAAA GTGGCTACGC 
GAGATCCTCA TCTGTCGGAA TACTTTTCTT CAGCGACACA AGGATTATGC ACATGTGGGA 
AGTCAGATTG CCATTTGCAA GCAGGCTCAC ATTAAGATGG AAGTTGTTTT CTTCATGTAC 
CTGTGGAGTG TTGATTTAGA TGCGGTGCTG ACATCGCTAT CGTGTTTTGG ACTACTATGC 
GAGGAGGCTG AGATTTGCTG CAGTTCTGAT GAATTGACAG TCGGATTTAT TATGCCGAAT 
TACCACATTT ACCAGGAGCT GGCTCAGTTG TCCACATCTG CAACGGACTC TCGCATTTGC 
TTTTTTGACA ACACGCACGG CAATGTGCTG AGCCGTCTTA CACTTCAAAA GCGCATAATG 
ACCCTATTGC GCAAGATCGA GCACTGTGTT CATGGTGTTC AGCCCGCCTG GGAGGAAACC 
TTCCGCAACT GGGAAGTATC CAGCAAGGTT TTGCAAACGT ACCCCAAATG TAAAGGAGAA 
GATGGCCAAG CGGAGGTTTT CCATCGTGGC ATGGGCAAGC GGCGAGCGAG TCACCAAAGC 
TCAGAGCACG ATTTGGAGGA ACAAATTAAT GAATGGGCCA ATATGACATG GTTTCTATTA 
GCCTTGGGTG GCGTCTGCCT TCATAAACGT AGCAGCAGTC GTCAAATGCT GCTGCAGCAA 
TCACAGAACA ATGCTTCTTT GGGATCACTT GCTCAAAACT CTCTTTACTC AAGCTCCACA 
AGTTCTGGAC ATGGCTCTCT GCATCCTAGC ACGGTTTCAC TATCCACTCT TCCCCCAGCA 
CCGCCACAAG ATGTCAGCTA TTGTCCTGTA ACACAATTCG TGGGCCAACT ATTGCGCCTG 
TTAGTTTGCA GCAACGAAAA AATTGGACTT AATATTCAGA AAAATGTGAA GGAACTGGTG 
GGCGAGGAGA TGTCCACCCA ACTGTATCCT ATACTCTTCG ACCAGGTTAG AGCCATCGTG 
GAGAAGTTCT TCGATCAGCA AGGTCAGGTG AACGTCAATG TGACCGACAT CAACACGCAG 
TTTATCGAGC ACACCATCTA CATAATGAAA TCAATTCTGG ATCCCAAAGC CAACAAGGAT 
CCCAACAACG ATCAACCCTC GCCATCGGAA CATTTAGGTG TTACAAGCAT CGAAGGCATG 
ATGCTAGGAA TAGTGCGCTA CGTTCGCCAC CTGGATATGA CTGTTTATGC CATTCGAATC 
AAGACAAAAT TGTGCCAGCT TGTGGAAGTA ATGATGAAGC GACGCGACGA CCTCGCCTTT 
CGCCAGGAGA TGAAGTTCCG GAATAAACTG GTTGAATACT TGACCGACTG GGTGATGGGC 
ACCTCCCATC AGATTGCACC GCCCAGCTCG GCGGATGCCG CTATTCTTAC CAACACATCC 
CTCATATTTC GCGATCTAGA CCAAGCCTGC ATGGAGGCAG TGGCTGCCTT GCTTCGGGGC 
CTTCCCCTTC AGCCTGAGGA ATCGGATCGA GGCGATTTGA TGGATGCAAA GAGTGCGCTC 
TTTTTAAAGT ACTTTACCCT ATTTATGAAC CTGCTGAATG ATTGCATCGA TAGCTCCGAG 
GCGGAGAAGG AAATGAATAA TACCCCACTA TTGCCTCCGC GCCCTCGAAT GGCAGCTGGA 
AAACTGACCG CTCTGCGAAA TGCCACTATT CTAGCCATGT CTAATTTGTT GGGTGCCAAC 
ATTGACTCTG GTTTGATGCA CTCCATCGAT CTGGGTTATA ATCCTGATTT GCAAACGCGT 
GCCGCTTTCA TGGAGGTGTT GACTCAAATC CTGCAACAAG GCACCGAATT CGATACCTTG 
GCCGAAACTG TTTTAGCTGA TCGATTTGAG CAACTGGTCC AACTTGTTAC AATGATCAGC 
GACAAAGGAG AACTTCCCAT AGCAATGGCT TTGGCTAATG TGGTGACTAC ATCCCAAATG 
GACGAGCTGG CTAGGGTTCT GGTCACCCTA TTCGACGCCA AACACCTGTT ATCGCCACTC 
CTATGGAATA TGTTTTATCG CGAGGTGGAG GTCTCAGACT GCATGCAGAC ACTTTTCCGT 
GGAAATTCTT TGGGCAGCAA AATCATGGCC TTTTGCTTTA AAATATACGG CGCGAGCTAT 
CTCCAAATGC TACTAGAGCC TTTAATTCGT CCGCTTCTGG ATGAGGAGGA GGAGACCTGT 
TTTGAGGTGG ATCCAGCTCG TCTGGATCCA ACAGAAGATA TTGAACAGCA CAGGAACAAC 
TTGATTGCCC TAACGCAGAA GGTGTTTGAC GCTATTATCA ACTCGTCGGA TCGCTTTCCC 
CCGCAGCTGC GATCCATGTG CCATTGCCTG TACCAAGTGC TAAGCAAACG CTTCCCGAAT 
CTTCTTCAAA ACAATATCGG TGCTGTAGGC ACAGTCATCT TCTTGCGGTT CATCAATCCC 
GCCATAGTTT CCCCACAGGA ATTGGGAATC GTTGACAAAC AAGTGCACAG CTCGGCCAAA 
CGAGGTCTAA TGCTAATGTC CAAGATCCTT CAAAACATTG CTAACCACGT GGAGTTCTCC 
AAGGAGCAGC ACATGTTGTG TTTCAACGAT TTTCTGCGCG ATCACTTTGA AGCTGGGCGG 
CGATTCTTCA TACAGATTGC ATCAGACTGT GAGACCGTGG ATCAGACCTC GCACAGCATG 
AGTTTTATTT CAGATGCGAA CGTACTGGCA CTGCATCGGT TGCTGTGGAC GCATCAGGAG 
AAGATCGGCG ACTATCTGTC TAGCAGTCGA GACCACAAGG CGGTTGGAAG GCGGCCCTTC 
GACAAGATGG CTACTTTGCT AGCATACCTG GGACCACCGG AGCACAAGCC CGTGGATTCG 
CACATGATGT TCAGCTCGTA TGCACGCTGG AGCTCCATTG ACATGTCGTC GACAAACTTC 
GAGGAGATCA TGGTCAAGCA CCAAATGCAC GAGAAGGAGG AATTCAAGAC CCTCAAGTCG 
ATGAACATAT TCTACCAGGC CGGAACAAGC AAATCTGGCT ATCCTGTCTT TTACTATATA 
GCAAGACGAT ACAAGATTGG AGAAACGAAT GGAGACTTAC TTATATATCA CGTCATACTC 
ACTCTGAAAC CATTCTGCCA CTCGCCCTTC GAGGTGGTCA TCGATTTTAC GCACACCTGT 
TCAGATAATC GGTTCCGCAC CGAGTTTCTG CAGAAGTGGT TTTATGTCCT GCCCACGGTA 
GCCTACGAAA ATGTCCATGC TGTGTACATC TATAACTGTA ACTCGTGGGT GCGCGAATAT 
ACCAAGTTCC ACGATCGCAT TCTGGCACCA TTAAAGGGAA ATCGCAAACT TCTGTTCCTG 
GAGTCACCCA ACAAACTTAC TGATTTCATC GACGCGGAGC AACAGAAATT GCCTGGAGCA 
ACTCTTTCCT TGGACGAAGA TTTAAAGGTA TTCAGCAACG CGCTGAAGCT CAGCCATAAA 
GACACAAAGG TGGCTATCAA AGTGGGTCCC ACCGCATTGC AAATTACATC TGCCGAAAAG 
ACAAAGGTCT TGGCTCACTC CGTGCTCCTG AACGATGTAT ACTACGCCTC TGAAATTGAG 
GAGGTGTGCT TGGTGGACGA TAACCAGTTC ACCCTGTCTA TAACTAACGA AAGTGGCCAG 
CTTAGCTTTA TTCACAACGA TTGCGACAAC ATTGTTCAAG CCATCATACA CATCCGAAAT 
CGATGGGAGC TTAGTCAGCC GGACTCCGTG ACGGTTCACC AAAAGATCCG ACCAAAAGAT 
GTGCCTGGAA CGCTGCTGAA CATGGCGCTA CTCAATCTGG GATCATGTGA CCCCAATCTA 
AGGACTGCTG CCTACAATTT GCTGTGTGCT TTGACCGCTA CTTTTGATCT AAAGATTGAG 
GGTCAACTGT TGGAGACCCA AGGACTTTGC ATACCCTCAA ATAATACCAT ATTTATCAAA 
TCCGTTAGCG AAAAGCTGGC CACCAATGAA CCGCATCTGA CTCTGGAATT CCTTGAGGAG 
TCCATTCAGG GCTTCCAGCG CAGCACCATA GAGCTGAAAC ATTTGTGTTT GGAGTACATG 
ACGCCATGGT TAAAGAACCT AGTCAAATTT TGCAAGTCCA ACGATGACTC CAAAAAGCTT 
AAGGTCTCCC AAATTCTGGA CAAGCTCATC AATTTAACTA TTGACCAAAA GGAAATGTAT 
CCCTCAGTGC AGGCCAAGAT CTGGGGATCA ATTGGACAGA TCCCCGAGCT GATCGACATG 
GTTTTGGATA ACTTTTTGCA CAAATCGATC ACATATGGCT TGGGATCACC ACAGGTGGAG 
ATTATGGCTG ACACGGCAGT GGCTCTCGCT TCAGCGAATG TTCAGCTGGT GTCCAAAAAG 
GTCATAACGC GAATATGCCG AGTCATGGAC AAATCCTGCA CAAATCCCAC ACAATATCTG 
GAGCAGCATA TGATGTGGGA CGATATTGCA ATTCTTGGTC GATACCTGCT CATGTTGTCG 
TTCAACAATT GTTTGGATGT GGCCACATCG GTGCCATATC TATTCCACAC CATTACGTTT 
TTGGTATGTT CAGGATCCCT GTCGATGCGA GCCTCTACTC ATGGCCTAGT GATCAACATC 
ATCCACTCGC TGTGCACATG CACAAATCCC TCATTTTCAG AGGAGGCGCA GCGAGTACTC 
CGACTGTCCC TGGATGAATT CTCACTGCCC AAGTTCTATC TACTCTTCGG CATCAGCAAG 
GTCAAGTCCG CAGCAGTTAC CGCTTTCCGC TCCAGCTGCC GTCACCCTAC AGATAAATGG 
CTAGGAAATG AAAGGGTTAC CCAGCCTCTG CCCGCCGATC GAGAGCGTTT ATCCCTACCA 
TCGCTTGAGG TTATCACGGA TGCCCTGCTG GAAATCATGG AGGCTTGCAT GCGAGATGTT 
CCGGACTGCG AATGGCTCAA CACCTGGACC TCTTTGGCTC GCAGTTTTGC TTTCTGTTAC 
AATCCAGCGC TACAGCCTAG AGCCCTTATA GTTTACGGGT GCATCAGCAA GAGTGTTACC 
GATCACGAGG TCAAGCAGTT ACTACGCATC CTGGTTAAGG CCCTCGAATC TTTCAACGAT 
CTCATTCTGA TCGAGGCTCT AGTAATGTGC TTAACTCGCA TTCAACCACT ACTCCGACCA 
GAGTCGCCCA TTCATCGGGC CCTCTTCTGG GTGGCCATTT CGGTGCTGCA GTTGGACGAG 
ATTACCCTGT ACGGCGCTGG ACTAGCTCTG CTTGAACAAA ATCTACACAC GCTTAAGTCA 
CAGGGCTGTT TTGACAAGAA GGAGACCATA GCCGAGGTCA TGATGAAGAC AAGGGAAAAG 
CTGGAGTGGC ATTTCAAGCA ACTGGATCAC GCCGTCGGCC TCTCCTTCCG CAGCAATTTC 
CACTTTGCCC TAGTGGGACA CTTGATTAAG GGCTTCCGCC ATCCTACACC TACAACCGTC 
TCACGCACCT CTCGTGTGCT GACGATGCTC TTGGGCATAA TAGCCAAACC CCTGCATCGG 
GACAAGTTCG AAGTAACACC TGACAGTGTG GCCTATTTGA CTGCGCTGGT GGCTGTCTCC 
GAGGAAGTCC GTTCCAGATG TCACGTAAAA CATGCCCTTC CCCGCTGGCC AGCCGATCTG 
AGTAGTAGTG TGGAAAATGG TGAAGCATCC GGCGGAGTGC AAGCTATCGG CCTGCCCCTG 
TCACGCCGTC AAAAAAGTTG GGACATCCTG GATCAGTCCG CCTTGCAGTT TGCCCGCCAA 
CACAAGGTTC CCACTCTTCA GGAACGCGGT TCGCGTTCGT CGGTGTCCAA CGAGTCCAAC 
GTACTGCTCG ATCCCGAGGT CTTACCTGAC CTGTCCATTC AAGCCCTGGT ACTGACTGTT 
TTGGCCACCT TGGTCAAGTA CTCTTCGGAT GAAGGCGAAA CGCGGGTTTT GTATCAGTAT 
TTGGCCGAAG GATCGGTGGT CTTTCCAAAG GTGTTTCCAG TTATCCATTC ACTGCTGGAC 
CAAAAGATAA ACAACATTTT GTCAGTGTCA CACGACCAAG TGGTGCTCAA CTCTGTGCAG 
AACATTATAC AAAATATGCT GGCCAGCGAA GATCCTTCCC AGCAGCAGCT GCACTTCCTG 
CAGAGTTGTG GATTTGGCGG ACTCTGGCGA TTTGCTGGTC CCTTCACCAA GTACAATATG 
ATGGGTGAGT CCTCGGAGCT GTTTGTCAAC TGTTTGGAGG CCATGGTTGA GACCTGTTTG 
CCAGGCGACG AATCCGCGCC GGTGCCACCA TCTCCGCGTC CATACAACCT GAGCTCCAGT 
CTGAGTAGCC TGACCCTGGG ATCACCCACG GATAAAGAAT GTCCAGAAGC GATGACCACT 
GAATGCGAAA GAAGGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733131.2
CDS	280..8520
Translation	MTQKPGEWASALLARFEDQLPNRIGAYGTQARMSQDQLVACLIHISRYRFSLVISGLTKMLQRVNEAALQNRHEPERCYFESLVIILTTLERCLTNQTKDTARFEEAMNVKLLLREISQFVDVQSDSNPNAAQLKALASKVLFALSQNHFSAVFNRISARIQELTSCSEENPDYNDIELIQHIDMDMIKLTKLLQETITKFRSKRAPPLILLYSLEKAIWNWIEYHPQEFQDLQRGTNRDISTCWEPLMDFVEYFKTENKKSKTLVWPLQMLLLILNPSCLEAVVNELQQSEKEKEKDKEKVASKSAQSTSRDKDFSAKQFIESIKRGLGQHSPSKQVTESAAIACVKLCKASTYINNTDSNNVVFKLVQFFINDLKALLFNPAKPFSRGQGYNFADIELMIDCWVSCFRINPHNIEALKVCLNLSSPQAYHFVIVCSLLRLAHIYVDFRLQNKNPFRIVNQPRLSWWPQTDVVHYRSAELRALFTDTLNKATQGYIAHTPLRYITSLTLKSKDTQKGLTRAEEGPAHKMLLLLLVRLIHADPTLLLNTQGKVAHEVQSSTLELINGLVSLVHQTTMPDVAQEAMEALLALHAPEKIEVWNPEAPINTFWDVSSQVLFSISQKLIQHQIANYTDVLKWLREILICRNTFLQRHKDYAHVGSQIAICKQAHIKMEVVFFMYLWSVDLDAVLTSLSCFGLLCEEAEICCSSDELTVGFIMPNYHIYQELAQLSTSATDSRICFFDNTHGNVLSRLTLQKRIMTLLRKIEHCVHGVQPAWEETFRNWEVSSKVLQTYPKCKGEDGQAEVFHRGMGKRRASHQSSEHDLEEQINEWANMTWFLLALGGVCLHKRSSSRQMLLQQSQNNASLGSLAQNSLYSSSTSSGHGSLHPSTVSLSTLPPAPPQDVSYCPVTQFVGQLLRLLVCSNEKIGLNIQKNVKELVGEEMSTQLYPILFDQVRAIVEKFFDQQGQVNVNVTDINTQFIEHTIYIMKSILDPKANKDPNNDQPSPSEHLGVTSIEGMMLGIVRYVRHLDMTVYAIRIKTKLCQLVEVMMKRRDDLAFRQEMKFRNKLVEYLTDWVMGTSHQIAPPSSADAAILTNTSLIFRDLDQACMEAVAALLRGLPLQPEESDRGDLMDAKSALFLKYFTLFMNLLNDCIDSSEAEKEMNNTPLLPPRPRMAAGKLTALRNATILAMSNLLGANIDSGLMHSIDLGYNPDLQTRAAFMEVLTQILQQGTEFDTLAETVLADRFEQLVQLVTMISDKGELPIAMALANVVTTSQMDELARVLVTLFDAKHLLSPLLWNMFYREVEVSDCMQTLFRGNSLGSKIMAFCFKIYGASYLQMLLEPLIRPLLDEEEETCFEVDPARLDPTEDIEQHRNNLIALTQKVFDAIINSSDRFPPQLRSMCHCLYQVLSKRFPNLLQNNIGAVGTVIFLRFINPAIVSPQELGIVDKQVHSSAKRGLMLMSKILQNIANHVEFSKEQHMLCFNDFLRDHFEAGRRFFIQIASDCETVDQTSHSMSFISDANVLALHRLLWTHQEKIGDYLSSSRDHKAVGRRPFDKMATLLAYLGPPEHKPVDSHMMFSSYARWSSIDMSSTNFEEIMVKHQMHEKEEFKTLKSMNIFYQAGTSKSGYPVFYYIARRYKIGETNGDLLIYHVILTLKPFCHSPFEVVIDFTHTCSDNRFRTEFLQKWFYVLPTVAYENVHAVYIYNCNSWVREYTKFHDRILAPLKGNRKLLFLESPNKLTDFIDAEQQKLPGATLSLDEDLKVFSNALKLSHKDTKVAIKVGPTALQITSAEKTKVLAHSVLLNDVYYASEIEEVCLVDDNQFTLSITNESGQLSFIHNDCDNIVQAIIHIRNRWELSQPDSVTVHQKIRPKDVPGTLLNMALLNLGSCDPNLRTAAYNLLCALTATFDLKIEGQLLETQGLCIPSNNTIFIKSVSEKLATNEPHLTLEFLEESIQGFQRSTIELKHLCLEYMTPWLKNLVKFCKSNDDSKKLKVSQILDKLINLTIDQKEMYPSVQAKIWGSIGQIPELIDMVLDNFLHKSITYGLGSPQVEIMADTAVALASANVQLVSKKVITRICRVMDKSCTNPTQYLEQHMMWDDIAILGRYLLMLSFNNCLDVATSVPYLFHTITFLVCSGSLSMRASTHGLVINIIHSLCTCTNPSFSEEAQRVLRLSLDEFSLPKFYLLFGISKVKSAAVTAFRSSCRHPTDKWLGNERVTQPLPADRERLSLPSLEVITDALLEIMEACMRDVPDCEWLNTWTSLARSFAFCYNPALQPRALIVYGCISKSVTDHEVKQLLRILVKALESFNDLILIEALVMCLTRIQPLLRPESPIHRALFWVAISVLQLDEITLYGAGLALLEQNLHTLKSQGCFDKKETIAEVMMKTREKLEWHFKQLDHAVGLSFRSNFHFALVGHLIKGFRHPTPTTVSRTSRVLTMLLGIIAKPLHRDKFEVTPDSVAYLTALVAVSEEVRSRCHVKHALPRWPADLSSSVENGEASGGVQAIGLPLSRRQKSWDILDQSALQFARQHKVPTLQERGSRSSVSNESNVLLDPEVLPDLSIQALVLTVLATLVKYSSDEGETRVLYQYLAEGSVVFPKVFPVIHSLLDQKINNILSVSHDQVVLNSVQNIIQNMLASEDPSQQQLHFLQSCGFGGLWRFAGPFTKYNMMGESSELFVNCLEAMVETCLPGDESAPVPPSPRPYNLSSSLSSLTLGSPTDKECPEAMTTECERRK

Target ORF information:

RefSeq Version	NM_170252.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neurofibromin 1, transcript variant C (Nf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170252.3

ORF Insert Sequence:

ATGACCCAGA AGCCAGGCGA GTGGGCTAGC GCCCTTTTGG CACGTTTCGA GGATCAGCTG 
CCAAACAGAA TCGGTGCCTA TGGCACGCAG GCCAGGATGA GCCAGGACCA ATTGGTGGCC 
TGTCTGATCC ACATATCGCG CTACCGCTTC TCCCTTGTCA TATCCGGTCT CACCAAGATG 
CTGCAGAGGG TCAATGAGGC GGCCCTTCAA AATCGCCATG AGCCAGAGCG TTGCTACTTT 
GAGTCGCTGG TCATCATACT GACCACTCTG GAACGCTGTC TGACCAACCA GACCAAGGAC 
ACAGCACGTT TTGAAGAGGC CATGAATGTG AAGCTTCTGC TGCGCGAGAT CTCCCAGTTT 
GTCGACGTCC AGAGCGACAG CAATCCGAAT GCCGCCCAGC TCAAGGCCCT GGCATCCAAG 
GTGCTCTTTG CCTTGTCCCA GAATCACTTC TCGGCGGTGT TCAATCGCAT ATCGGCCAGG 
ATTCAAGAGC TGACATCCTG TTCCGAGGAA AACCCGGACT ACAACGACAT AGAATTAATT 
CAACACATCG ATATGGATAT GATAAAGCTA ACCAAGCTGC TACAAGAAAC CATCACAAAG 
TTTCGATCGA AGCGTGCTCC ACCTTTGATC TTGCTTTACT CGCTGGAGAA GGCTATTTGG 
AACTGGATCG AGTACCATCC ACAAGAGTTC CAGGACTTGC AACGAGGAAC CAATCGAGAT 
ATATCAACCT GCTGGGAACC CCTGATGGAC TTTGTAGAGT ACTTTAAAAC CGAAAACAAG 
AAAAGCAAGA CTCTTGTTTG GCCGCTTCAA ATGCTCTTGC TGATATTGAA TCCCTCTTGC 
CTTGAGGCTG TTGTTAACGA ACTTCAGCAG TCGGAGAAAG AGAAGGAAAA GGACAAGGAA 
AAGGTTGCCT CGAAGTCAGC GCAATCCACG TCCCGGGACA AGGACTTCTC GGCCAAGCAG 
TTCATCGAGA GTATCAAGAG AGGCTTGGGC CAGCACTCAC CATCCAAACA GGTGACCGAA 
TCTGCGGCAA TTGCCTGTGT AAAGCTGTGC AAAGCATCCA CCTACATCAA CAACACCGAC 
TCCAATAATG TGGTCTTCAA GTTGGTGCAA TTCTTTATCA ACGATCTCAA GGCACTGCTC 
TTTAATCCAG CAAAGCCCTT TTCGCGCGGT CAGGGGTACA ACTTCGCTGA TATCGAGCTG 
ATGATTGACT GCTGGGTGTC TTGTTTCCGG ATTAATCCTC ACAACATCGA GGCTCTAAAG 
GTTTGCCTGA ATTTGTCCTC GCCGCAGGCT TACCATTTTG TAATTGTGTG CTCTCTGCTA 
AGGTTGGCTC ACATATACGT TGACTTCCGT TTGCAGAACA AGAACCCCTT TCGAATAGTA 
AACCAACCGA GATTGTCTTG GTGGCCGCAA ACGGATGTGG TTCACTACCG TTCTGCTGAG 
CTACGAGCTT TGTTTACGGA TACGCTCAAC AAGGCCACCC AGGGTTATAT AGCCCATACG 
CCACTGCGCT ACATAACCTC CCTGACGCTC AAATCTAAAG ATACCCAGAA AGGTTTAACC 
CGCGCTGAAG AGGGTCCCGC CCACAAAATG CTATTACTGC TACTCGTACG GCTAATTCAT 
GCAGATCCCA CGCTTTTGTT GAATACCCAA GGAAAAGTGG CACATGAAGT GCAGAGCTCC 
ACATTGGAGC TCATCAACGG CTTAGTTAGT TTGGTGCATC AAACTACCAT GCCCGATGTG 
GCACAAGAGG CTATGGAGGC GCTGCTTGCT CTGCACGCCC CGGAGAAAAT AGAGGTTTGG 
AATCCAGAGG CTCCGATCAA TACGTTCTGG GACGTTAGCT CCCAAGTACT GTTTTCAATC 
TCGCAAAAGC TCATTCAACA CCAAATTGCC AATTACACTG ATGTCTTAAA GTGGCTACGC 
GAGATCCTCA TCTGTCGGAA TACTTTTCTT CAGCGACACA AGGATTATGC ACATGTGGGA 
AGTCAGATTG CCATTTGCAA GCAGGCTCAC ATTAAGATGG AAGTTGTTTT CTTCATGTAC 
CTGTGGAGTG TTGATTTAGA TGCGGTGCTG ACATCGCTAT CGTGTTTTGG ACTACTATGC 
GAGGAGGCTG AGATTTGCTG CAGTTCTGAT GAATTGACAG TCGGATTTAT TATGCCGAAT 
TACCACATTT ACCAGGAGCT GGCTCAGTTG TCCACATCTG CAACGGACTC TCGCATTTGC 
TTTTTTGACA ACACGCACGG CAATGTGCTG AGCCGTCTTA CACTTCAAAA GCGCATAATG 
ACCCTATTGC GCAAGATCGA GCACTGTGTT CATGGTGTTC AGCCCGCCTG GGAGGAAACC 
TTCCGCAACT GGGAAGTATC CAGCAAGGTT TTGCAAACGT ACCCCAAATG TAAAGGAGAA 
GATGGCCAAG CGGAGGTTTT CCATCGTGGC ATGGGCAAGC GGCGAGCGAG TCACCAAAGC 
TCAGAGCACG ATTTGGAGGA ACAAATTAAT GAATGGGCCA ATATGACATG GTTTCTATTA 
GCCTTGGGTG GCGTCTGCCT TCATAAACGT AGCAGCAGTC GTCAAATGCT GCTGCAGCAA 
TCACAGAACA ATGCTTCTTT GGGATCACTT GCTCAAAACT CTCTTTACTC AAGCTCCACA 
AGTTCTGGAC ATGGCTCTCT GCATCCTAGC ACGGTTTCAC TATCCACTCT TCCCCCAGCA 
CCGCCACAAG ATGTCAGCTA TTGTCCTGTA ACACAATTCG TGGGCCAACT ATTGCGCCTG 
TTAGTTTGCA GCAACGAAAA AATTGGACTT AATATTCAGA AAAATGTGAA GGAACTGGTG 
GGCGAGGAGA TGTCCACCCA ACTGTATCCT ATACTCTTCG ACCAGGTTAG AGCCATCGTG 
GAGAAGTTCT TCGATCAGCA AGGTCAGGTG AACGTCAATG TGACCGACAT CAACACGCAG 
TTTATCGAGC ACACCATCTA CATAATGAAA TCAATTCTGG ATCCCAAAGC CAACAAGGAT 
CCCAACAACG ATCAACCCTC GCCATCGGAA CATTTAGGTG TTACAAGCAT CGAAGGCATG 
ATGCTAGGAA TAGTGCGCTA CGTTCGCCAC CTGGATATGA CTGTTTATGC CATTCGAATC 
AAGACAAAAT TGTGCCAGCT TGTGGAAGTA ATGATGAAGC GACGCGACGA CCTCGCCTTT 
CGCCAGGAGA TGAAGTTCCG GAATAAACTG GTTGAATACT TGACCGACTG GGTGATGGGC 
ACCTCCCATC AGATTGCACC GCCCAGCTCG GCGGATGCCG CTATTCTTAC CAACACATCC 
CTCATATTTC GCGATCTAGA CCAAGCCTGC ATGGAGGCAG TGGCTGCCTT GCTTCGGGGC 
CTTCCCCTTC AGCCTGAGGA ATCGGATCGA GGCGATTTGA TGGATGCAAA GAGTGCGCTC 
TTTTTAAAGT ACTTTACCCT ATTTATGAAC CTGCTGAATG ATTGCATCGA TAGCTCCGAG 
GCGGAGAAGG AAATGAATAA TACCCCACTA TTGCCTCCGC GCCCTCGAAT GGCAGCTGGA 
AAACTGACCG CTCTGCGAAA TGCCACTATT CTAGCCATGT CTAATTTGTT GGGTGCCAAC 
ATTGACTCTG GTTTGATGCA CTCCATCGAT CTGGGTTATA ATCCTGATTT GCAAACGCGT 
GCCGCTTTCA TGGAGGTGTT GACTCAAATC CTGCAACAAG GCACCGAATT CGATACCTTG 
GCCGAAACTG TTTTAGCTGA TCGATTTGAG CAACTGGTCC AACTTGTTAC AATGATCAGC 
GACAAAGGAG AACTTCCCAT AGCAATGGCT TTGGCTAATG TGGTGACTAC ATCCCAAATG 
GACGAGCTGG CTAGGGTTCT GGTCACCCTA TTCGACGCCA AACACCTGTT ATCGCCACTC 
CTATGGAATA TGTTTTATCG CGAGGTGGAG GTCTCAGACT GCATGCAGAC ACTTTTCCGT 
GGAAATTCTT TGGGCAGCAA AATCATGGCC TTTTGCTTTA AAATATACGG CGCGAGCTAT 
CTCCAAATGC TACTAGAGCC TTTAATTCGT CCGCTTCTGG ATGAGGAGGA GGAGACCTGT 
TTTGAGGTGG ATCCAGCTCG TCTGGATCCA ACAGAAGATA TTGAACAGCA CAGGAACAAC 
TTGATTGCCC TAACGCAGAA GGTGTTTGAC GCTATTATCA ACTCGTCGGA TCGCTTTCCC 
CCGCAGCTGC GATCCATGTG CCATTGCCTG TACCAAGTGC TAAGCAAACG CTTCCCGAAT 
CTTCTTCAAA ACAATATCGG TGCTGTAGGC ACAGTCATCT TCTTGCGGTT CATCAATCCC 
GCCATAGTTT CCCCACAGGA ATTGGGAATC GTTGACAAAC AAGTGCACAG CTCGGCCAAA 
CGAGGTCTAA TGCTAATGTC CAAGATCCTT CAAAACATTG CTAACCACGT GGAGTTCTCC 
AAGGAGCAGC ACATGTTGTG TTTCAACGAT TTTCTGCGCG ATCACTTTGA AGCTGGGCGG 
CGATTCTTCA TACAGATTGC ATCAGACTGT GAGACCGTGG ATCAGACCTC GCACAGCATG 
AGTTTTATTT CAGATGCGAA CGTACTGGCA CTGCATCGGT TGCTGTGGAC GCATCAGGAG 
AAGATCGGCG ACTATCTGTC TAGCAGTCGA GACCACAAGG CGGTTGGAAG GCGGCCCTTC 
GACAAGATGG CTACTTTGCT AGCATACCTG GGACCACCGG AGCACAAGCC CGTGGATTCG 
CACATGATGT TCAGCTCGTA TGCACGCTGG AGCTCCATTG ACATGTCGTC GACAAACTTC 
GAGGAGATCA TGGTCAAGCA CCAAATGCAC GAGAAGGAGG AATTCAAGAC CCTCAAGTCG 
ATGAACATAT TCTACCAGGC CGGAACAAGC AAATCTGGCT ATCCTGTCTT TTACTATATA 
GCAAGACGAT ACAAGATTGG AGAAACGAAT GGAGACTTAC TTATATATCA CGTCATACTC 
ACTCTGAAAC CATTCTGCCA CTCGCCCTTC GAGGTGGTCA TCGATTTTAC GCACACCTGT 
TCAGATAATC GGTTCCGCAC CGAGTTTCTG CAGAAGTGGT TTTATGTCCT GCCCACGGTA 
GCCTACGAAA ATGTCCATGC TGTGTACATC TATAACTGTA ACTCGTGGGT GCGCGAATAT 
ACCAAGTTCC ACGATCGCAT TCTGGCACCA TTAAAGGGAA ATCGCAAACT TCTGTTCCTG 
GAGTCACCCA ACAAACTTAC TGATTTCATC GACGCGGAGC AACAGAAATT GCCTGGAGCA 
ACTCTTTCCT TGGACGAAGA TTTAAAGGTA TTCAGCAACG CGCTGAAGCT CAGCCATAAA 
GACACAAAGG TGGCTATCAA AGTGGGTCCC ACCGCATTGC AAATTACATC TGCCGAAAAG 
ACAAAGGTCT TGGCTCACTC CGTGCTCCTG AACGATGTAT ACTACGCCTC TGAAATTGAG 
GAGGTGTGCT TGGTGGACGA TAACCAGTTC ACCCTGTCTA TAACTAACGA AAGTGGCCAG 
CTTAGCTTTA TTCACAACGA TTGCGACAAC ATTGTTCAAG CCATCATACA CATCCGAAAT 
CGATGGGAGC TTAGTCAGCC GGACTCCGTG ACGGTTCACC AAAAGATCCG ACCAAAAGAT 
GTGCCTGGAA CGCTGCTGAA CATGGCGCTA CTCAATCTGG GATCATGTGA CCCCAATCTA 
AGGACTGCTG CCTACAATTT GCTGTGTGCT TTGACCGCTA CTTTTGATCT AAAGATTGAG 
GGTCAACTGT TGGAGACCCA AGGACTTTGC ATACCCTCAA ATAATACCAT ATTTATCAAA 
TCCGTTAGCG AAAAGCTGGC CACCAATGAA CCGCATCTGA CTCTGGAATT CCTTGAGGAG 
TCCATTCAGG GCTTCCAGCG CAGCACCATA GAGCTGAAAC ATTTGTGTTT GGAGTACATG 
ACGCCATGGT TAAAGAACCT AGTCAAATTT TGCAAGTCCA ACGATGACTC CAAAAAGCTT 
AAGGTCTCCC AAATTCTGGA CAAGCTCATC AATTTAACTA TTGACCAAAA GGAAATGTAT 
CCCTCAGTGC AGGCCAAGAT CTGGGGATCA ATTGGACAGA TCCCCGAGCT GATCGACATG 
GTTTTGGATA ACTTTTTGCA CAAATCGATC ACATATGGCT TGGGATCACC ACAGGTGGAG 
ATTATGGCTG ACACGGCAGT GGCTCTCGCT TCAGCGAATG TTCAGCTGGT GTCCAAAAAG 
GTCATAACGC GAATATGCCG AGTCATGGAC AAATCCTGCA CAAATCCCAC ACAATATCTG 
GAGCAGCATA TGATGTGGGA CGATATTGCA ATTCTTGGTC GATACCTGCT CATGTTGTCG 
TTCAACAATT GTTTGGATGT GGCCACATCG GTGCCATATC TATTCCACAC CATTACGTTT 
TTGGTATGTT CAGGATCCCT GTCGATGCGA GCCTCTACTC ATGGCCTAGT GATCAACATC 
ATCCACTCGC TGTGCACATG CACAAATCCC TCATTTTCAG AGGAGGCGCA GCGAGTACTC 
CGACTGTCCC TGGATGAATT CTCACTGCCC AAGTTCTATC TACTCTTCGG CATCAGCAAG 
GTCAAGTCCG CAGCAGTTAC CGCTTTCCGC TCCAGCTGCC GTCACCCTAC AGATAAATGG 
CTAGGAAATG AAAGGGTTAC CCAGCCTCTG CCCGCCGATC GAGAGCGTTT ATCCCTACCA 
TCGCTTGAGG TTATCACGGA TGCCCTGCTG GAAATCATGG AGGCTTGCAT GCGAGATGTT 
CCGGACTGCG AATGGCTCAA CACCTGGACC TCTTTGGCTC GCAGTTTTGC TTTCTGTTAC 
AATCCAGCGC TACAGCCTAG AGCCCTTATA GTTTACGGGT GCATCAGCAA GAGTGTTACC 
GATCACGAGG TCAAGCAGTT ACTACGCATC CTGGTTAAGG CCCTCGAATC TTTCAACGAT 
CTCATTCTGA TCGAGGCTCT AGTAATGTGC TTAACTCGCA TTCAACCACT ACTCCGACCA 
GAGTCGCCCA TTCATCGGGC CCTCTTCTGG GTGGCCATTT CGGTGCTGCA GTTGGACGAG 
ATTACCCTGT ACGGCGCTGG ACTAGCTCTG CTTGAACAAA ATCTACACAC GCTTAAGTCA 
CAGGGCTGTT TTGACAAGAA GGAGACCATA GCCGAGGTCA TGATGAAGAC AAGGGAAAAG 
CTGGAGTGGC ATTTCAAGCA ACTGGATCAC GCCGTCGGCC TCTCCTTCCG CAGCAATTTC 
CACTTTGCCC TAGTGGGACA CTTGATTAAG GGCTTCCGCC ATCCTACACC TACAACCGTC 
TCACGCACCT CTCGTGTGCT GACGATGCTC TTGGGCATAA TAGCCAAACC CCTGCATCGG 
GACAAGTTCG AAGTAACACC TGACAGTGTG GCCTATTTGA CTGCGCTGGT GGCTGTCTCC 
GAGGAAGTCC GTTCCAGATG TCACGTAAAA CATGCCCTTC CCCGCTGGCC AGCCGATCTG 
AGTAGTAGTG TGGAAAATGG TGAAGCATCC GGCGGAGTGC AAGCTATCGG CCTGCCCCTG 
TCACGCCGTC AAAAAAGTTG GGACATCCTG GATCAGTCCG CCTTGCAGTT TGCCCGCCAA 
CACAAGGTTC CCACTCTTCA GGAACGCGGT TCGCGTTCGT CGGTGTCCAA CGAGTCCAAC 
GTACTGCTCG ATCCCGAGGT CTTACCTGAC CTGTCCATTC AAGCCCTGGT ACTGACTGTT 
TTGGCCACCT TGGTCAAGTA CTCTTCGGAT GAAGGCGAAA CGCGGGTTTT GTATCAGTAT 
TTGGCCGAAG GATCGGTGGT CTTTCCAAAG GTGTTTCCAG TTATCCATTC ACTGCTGGAC 
CAAAAGATAA ACAACATTTT GTCAGTGTCA CACGACCAAG TGGTGCTCAA CTCTGTGCAG 
AACATTATAC AAAATATGCT GGCCAGCGAA GATCCTTCCC AGCAGCAGCT GCACTTCCTG 
CAGAGTTGTG GATTTGGCGG ACTCTGGCGA TTTGCTGGTC CCTTCACCAA GTACAATATG 
ATGGGTGAGT CCTCGGAGCT GTTTGTCAAC TGTTTGGAGG CCATGGTTGA GACCTGTTTG 
CCAGGCGACG AATCCGCGCC GGTGCCACCA TCTCCGCGTC CATACAACCT GAGCTCCAGT 
CTGAGTAGCC TGACCCTGGG ATCACCCACG GATAAAGAAT GTCCAGAAGC GATGACCACT 
GAATGCGAAA GAAGGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nf1 ( NM_170253.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733132.2 Drosophila melanogaster neurofibromin 1, transcript variant B (Nf1), mRNA.

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CloneID	OFa06162
Clone ID Related Accession (Same CDS sequence)	NM_170253.2
Accession Version	NM_170253.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neurofibromin 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Mar 18, 2004 this sequence version replaced NM_170253.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCCAGA AGCCAGGCGA GTGGGCTAGC GCCCTTTTGG CACGTTTCGA GGATCAGCTG 
CCAAACAGAA TCGGTGCCTA TGGCACGCAG GCCAGGATGA GCCAGGACCA ATTGGTGGCC 
TGTCTGATCC ACATATCGCG CTACCGCTTC TCCCTTGTCA TATCCGGTCT CACCAAGATG 
CTGCAGAGGG TCAATGAGGC GGCCCTTCAA AATCGCCATG AGCCAGAGCG TTGCTACTTT 
GAGTCGCTGG TCATCATACT GACCACTCTG GAACGCTGTC TGACCAACCA GACCAAGGAC 
ACAGCACGTT TTGAAGAGGC CATGAATGTG AAGCTTCTGC TGCGCGAGAT CTCCCAGTTT 
GTCGACGTCC AGAGCGACAG CAATCCGAAT GCCGCCCAGC TCAAGGCCCT GGCATCCAAG 
GTGCTCTTTG CCTTGTCCCA GAATCACTTC TCGGCGGTGT TCAATCGCAT ATCGGCCAGG 
ATTCAAGAGC TGACATCCTG TTCCGAGGAA AACCCGGACT ACAACGACAT AGAATTAATT 
CAACACATCG ATATGGATAT GATAAAGCTA ACCAAGCTGC TACAAGAAAC CATCACAAAG 
TTTCGATCGA AGCGTGCTCC ACCTTTGATC TTGCTTTACT CGCTGGAGAA GGCTATTTGG 
AACTGGATCG AGTACCATCC ACAAGAGTTC CAGGACTTGC AACGAGGAAC CAATCGAGAT 
ATATCAACCT GCTGGGAACC CCTGATGGAC TTTGTAGAGT ACTTTAAAAC CGAAAACAAG 
AAAAGCAAGA CTCTTGTTTG GCCGCTTCAA ATGCTCTTGC TGATATTGAA TCCCTCTTGC 
CTTGAGGCTG TTGTTAACGA ACTTCAGCAG TCGGAGAAAG AGAAGGAAAA GGACAAGGAA 
AAGGTTGCCT CGAAGTCAGC GCAATCCACG TCCCGGGACA AGGACTTCTC GGCCAAGCAG 
TTCATCGAGA GTATCAAGAG AGGCTTGGGC CAGCACTCAC CATCCAAACA GGTGACCGAA 
TCTGCGGCAA TTGCCTGTGT AAAGCTGTGC AAAGCATCCA CCTACATCAA CAACACCGAC 
TCCAATAATG TGGTCTTCAA GTTGGTGCAA TTCTTTATCA ACGATCTCAA GGCACTGCTC 
TTTAATCCAG CAAAGCCCTT TTCGCGCGGT CAGGGGTACA ACTTCGCTGA TATCGAGCTG 
ATGATTGACT GCTGGGTGTC TTGTTTCCGG ATTAATCCTC ACAACATCGA GGCTCTAAAG 
GTTTGCCTGA ATTTGTCCTC GCCGCAGGCT TACCATTTTG TAATTGTGTG CTCTCTGCTA 
AGGTTGGCTC ACATATACGT TGACTTCCGT TTGCAGAACA AGAACCCCTT TCGAATAGTA 
AACCAACCGA GATTGTCTTG GTGGCCGCAA ACGGATGTGG TTCACTACCG TTCTGCTGAG 
CTACGAGCTT TGTTTACGGA TACGCTCAAC AAGGCCACCC AGGGTTATAT AGCCCATACG 
CCACTGCGCT ACATAACCTC CCTGACGCTC AAATCTAAAG ATACCCAGAA AGGTTTAACC 
CGCGCTGAAG AGGGTCCCGC CCACAAAATG CTATTACTGC TACTCGTACG GCTAATTCAT 
GCAGATCCCA CGCTTTTGTT GAATACCCAA GGAAAAGTGG CACATGAAGT GCAGAGCTCC 
ACATTGGAGC TCATCAACGG CTTAGTTAGT TTGGTGCATC AAACTACCAT GCCCGATGTG 
GCACAAGAGG CTATGGAGGC GCTGCTTGCT CTGCACGCCC CGGAGAAAAT AGAGGTTTGG 
AATCCAGAGG CTCCGATCAA TACGTTCTGG GACGTTAGCT CCCAAGTACT GTTTTCAATC 
TCGCAAAAGC TCATTCAACA CCAAATTGCC AATTACACTG ATGTCTTAAA GTGGCTACGC 
GAGATCCTCA TCTGTCGGAA TACTTTTCTT CAGCGACACA AGGATTATGC ACATGTGGGA 
AGTCAGATTG CCATTTGCAA GCAGGCTCAC ATTAAGATGG AAGTTGTTTT CTTCATGTAC 
CTGTGGAGTG TTGATTTAGA TGCGGTGCTG ACATCGCTAT CGTGTTTTGG ACTACTATGC 
GAGGAGGCTG AGATTTGCTG CAGTTCTGAT GAATTGACAG TCGGATTTAT TATGCCGAAT 
TACCACATTT ACCAGGAGCT GGCTCAGTTG TCCACATCTG CAACGGACTC TCGCATTTGC 
TTTTTTGACA ACACGCACGG CAATGTGCTG AGCCGTCTTA CACTTCAAAA GCGCATAATG 
ACCCTATTGC GCAAGATCGA GCACTGTGTT CATGGTGTTC AGCCCGCCTG GGAGGAAACC 
TTCCGCAACT GGGAAGTATC CAGCAAGGTT TTGCAAACGT ACCCCAAATG TAAAGGAGAA 
GATGGCCAAG CGGAGGTTTT CCATCGTGGC ATGGGCAAGC GGCGAGCGAG TCACCAAAGC 
TCAGAGCACG ATTTGGAGGA ACAAATTAAT GAATGGGCCA ATATGACATG GTTTCTATTA 
GCCTTGGGTG GCGTCTGCCT TCATAAACGT AGCAGCAGTC GTCAAATGCT GCTGCAGCAA 
TCACAGAACA ATGCTTCTTT GGGATCACTT GCTCAAAACT CTCTTTACTC AAGCTCCACA 
AGTTCTGGAC ATGGCTCTCT GCATCCTAGC ACGGTTTCAC TATCCACTCT TCCCCCAGCA 
CCGCCACAAG ATGTCAGCTA TTGTCCTGTA ACACAATTCG TGGGCCAACT ATTGCGCCTG 
TTAGTTTGCA GCAACGAAAA AATTGGACTT AATATTCAGA AAAATGTGAA GGAACTGGTG 
GGCGAGGAGA TGTCCACCCA ACTGTATCCT ATACTCTTCG ACCAGGTTAG AGCCATCGTG 
GAGAAGTTCT TCGATCAGCA AGGTCAGGTG AACGTCAATG TGACCGACAT CAACACGCAG 
TTTATCGAGC ACACCATCTA CATAATGAAA TCAATTCTGG ATCCCAAAGC CAACAAGGAT 
CCCAACAACG ATCAACCCTC GCCATCGGAA CATTTAGGTG TTACAAGCAT CGAAGGCATG 
ATGCTAGGAA TAGTGCGCTA CGTTCGCCAC CTGGATATGA CTGTTTATGC CATTCGAATC 
AAGACAAAAT TGTGCCAGCT TGTGGAAGTA ATGATGAAGC GACGCGACGA CCTCGCCTTT 
CGCCAGGAGA TGAAGTTCCG GAATAAACTG GTTGAATACT TGACCGACTG GGTGATGGGC 
ACCTCCCATC AGATTGCACC GCCCAGCTCG GCGGATGCCG CTATTCTTAC CAACACATCC 
CTCATATTTC GCGATCTAGA CCAAGCCTGC ATGGAGGCAG TGGCTGCCTT GCTTCGGGGC 
CTTCCCCTTC AGCCTGAGGA ATCGGATCGA GGCGATTTGA TGGATGCAAA GAGTGCGCTC 
TTTTTAAAGT ACTTTACCCT ATTTATGAAC CTGCTGAATG ATTGCATCGA TAGCTCCGAG 
GCGGAGAAGG AAATGAATAA TACCCCACTA TTGCCTCCGC GCCCTCGAAT GGCAGCTGGA 
AAACTGACCG CTCTGCGAAA TGCCACTATT CTAGCCATGT CTAATTTGTT GGGTGCCAAC 
ATTGACTCTG GTTTGATGCA CTCCATCGAT CTGGGTTATA ATCCTGATTT GCAAACGCGT 
GCCGCTTTCA TGGAGGTGTT GACTCAAATC CTGCAACAAG GCACCGAATT CGATACCTTG 
GCCGAAACTG TTTTAGCTGA TCGATTTGAG CAACTGGTCC AACTTGTTAC AATGATCAGC 
GACAAAGGAG AACTTCCCAT AGCAATGGCT TTGGCTAATG TGGTGACTAC ATCCCAAATG 
GACGAGCTGG CTAGGGTTCT GGTCACCCTA TTCGACGCCA AACACCTGTT ATCGCCACTC 
CTATGGAATA TGTTTTATCG CGAGGTGGAG GTCTCAGACT GCATGCAGAC ACTTTTCCGT 
GGAAATTCTT TGGGCAGCAA AATCATGGCC TTTTGCTTTA AAATATACGG CGCGAGCTAT 
CTCCAAATGC TACTAGAGCC TTTAATTCGT CCGCTTCTGG ATGAGGAGGA GGAGACCTGT 
TTTGAGGTGG ATCCAGCTCG TCTGGATCCA ACAGAAGATA TTGAACAGCA CAGGAACAAC 
TTGATTGCCC TAACGCAGAA GGTGTTTGAC GCTATTATCA ACTCGTCGGA TCGCTTTCCC 
CCGCAGCTGC GATCCATGTG CCATTGCCTG TACCAAGTGC TAAGCAAACG CTTCCCGAAT 
CTTCTTCAAA ACAATATCGG TGCTGTAGGC ACAGTCATCT TCTTGCGGTT CATCAATCCC 
GCCATAGTTT CCCCACAGGA ATTGGGAATC GTTGACAAAC AAGTGCACAG CTCGGCCAAA 
CGAGGTCTAA TGCTAATGTC CAAGATCCTT CAAAACATTG CTAACCACGT GGAGTTCTCC 
AAGGAGCAGC ACATGTTGTG TTTCAACGAT TTTCTGCGCG ATCACTTTGA AGCTGGGCGG 
CGATTCTTCA TACAGATTGC ATCAGACTGT GAGACCGTGG ATCAGACCTC GCACAGCATG 
AGTTTTATTT CAGATGCGAA CGTACTGGCA CTGCATCGGT TGCTGTGGAC GCATCAGGAG 
AAGATCGGCG ACTATCTGTC TAGCAGTCGA GACCACAAGG CGGTTGGAAG GCGGCCCTTC 
GACAAGATGG CTACTTTGCT AGCATACCTG GGACCACCGG AGCACAAGCC CGTGGATTCG 
CACATGATGT TCAGCTCGTA TGCACGCTGG AGCTCCATTG ACATGTCGTC GACAAACTTC 
GAGGAGATCA TGGTCAAGCA CCAAATGCAC GAGAAGGAGG AATTCAAGAC CCTCAAGTCG 
ATGAACATAT TCTACCAGGC CGGAACAAGC AAATCTGGCT ATCCTGTCTT TTACTATATA 
GCAAGACGAT ACAAGATTGG AGAAACGAAT GGAGACTTAC TTATATATCA CGTCATACTC 
ACTCTGAAAC CATTCTGCCA CTCGCCCTTC GAGGTGGTCA TCGATTTTAC GCACACCTGT 
TCAGATAATC GGTTCCGCAC CGAGTTTCTG CAGAAGTGGT TTTATGTCCT GCCCACGGTA 
GCCTACGAAA ATGTCCATGC TGTGTACATC TATAACTGTA ACTCGTGGGT GCGCGAATAT 
ACCAAGTTCC ACGATCGCAT TCTGGCACCA TTAAAGGGAA ATCGCAAACT TCTGTTCCTG 
GAGTCACCCA ACAAACTTAC TGATTTCATC GACGCGGAGC AACAGAAATT GCCTGGAGCA 
ACTCTTTCCT TGGACGAAGA TTTAAAGGTA TTCAGCAACG CGCTGAAGCT CAGCCATAAA 
GACACAAAGG TGGCTATCAA AGTGGGTCCC ACCGCATTGC AAATTACATC TGCCGAAAAG 
ACAAAGGTCT TGGCTCACTC CGTGCTCCTG AACGATGTAT ACTACGCCTC TGAAATTGAG 
GAGGTGTGCT TGGTGGACGA TAACCAGTTC ACCCTGTCTA TAACTAACGA AAGTGGCCAG 
CTTAGCTTTA TTCACAACGA TTGCGACAAC ATTGTTCAAG CCATCATACA CATCCGAAAT 
CGATGGGAGC TTAGTCAGCC GGACTCCGTG ACGGTTCACC AAAAGATCCG ACCAAAAGAT 
GTGCCTGGAA CGCTGCTGAA CATGGCGCTA CTCAATCTGG GATCATGTGA CCCCAATCTA 
AGGACTGCTG CCTACAATTT GCTGTGTGCT TTGACCGCTA CTTTTGATCT AAAGATTGAG 
GGTCAACTGT TGGAGACCCA AGGACTTTGC ATACCCTCAA ATAATACCAT ATTTATCAAA 
TCCGTTAGCG AAAAGCTGGC CACCAATGAA CCGCATCTGA CTCTGGAATT CCTTGAGGAG 
TCCATTCAGG GCTTCCAGCG CAGCACCATA GAGCTGAAAC ATTTGTGTTT GGAGTACATG 
ACGCCATGGT TAAAGAACCT AGTCAAATTT TGCAAGTCCA ACGATGACTC CAAAAAGCTT 
AAGGTCTCCC AAATTCTGGA CAAGCTCATC AATTTAACTA TTGACCAAAA GGAAATGTAT 
CCCTCAGTGC AGGCCAAGAT CTGGGGATCA ATTGGACAGA TCCCCGAGCT GATCGACATG 
GTTTTGGATA ACTTTTTGCA CAAATCGATC ACATATGGCT TGGGATCACC ACAGGTGGAG 
ATTATGGCTG ACACGGCAGT GGCTCTCGCT TCAGCGAATG TTCAGCTGGT GTCCAAAAAG 
GTCATAACGC GAATATGCCG AGTCATGGAC AAATCCTGCA CAAATCCCAC ACAATATCTG 
GAGCAGCATA TGATGTGGGA CGATATTGCA ATTCTTGGTC GATACCTGCT CATGTTGTCG 
TTCAACAATT GTTTGGATGT GGCCACATCG GTGCCATATC TATTCCACAC CATTACGTTT 
TTGGTATGTT CAGGATCCCT GTCGATGCGA GCCTCTACTC ATGGCCTAGT GATCAACATC 
ATCCACTCGC TGTGCACATG CACAAATCCC TCATTTTCAG AGGAGGCGCA GCGAGTACTC 
CGACTGTCCC TGGATGAATT CTCACTGCCC AAGTTCTATC TACTCTTCGG CATCAGCAAG 
GTCAAGTCCG CAGCAGTTAC CGCTTTCCGC TCCAGCTGCC GTCACCCTAC AGATAAATGG 
CTAGGAAATG AAAGGGTTAC CCAGCCTCTG CCCGCCGATC GAGAGCGTTT ATCCCTACCA 
TCGCTTGAGG TTATCACGGA TGCCCTGCTG GAAATCATGG AGGCTTGCAT GCGAGATGTT 
CCGGACTGCG AATGGCTCAA CACCTGGACC TCTTTGGCTC GCAGTTTTGC TTTCTGTTAC 
AATCCAGCGC TACAGCCTAG AGCCCTTATA GTTTACGGGT GCATCAGCAA GAGTGTTACC 
GATCACGAGG TCAAGCAGTT ACTACGCATC CTGGTTAAGG CCCTCGAATC TTTCAACGAT 
CTCATTCTGA TCGAGGCTCT AGTAATGTGC TTAACTCGCA TTCAACCACT ACTCCGACCA 
GAGTCGCCCA TTCATCGGGC CCTCTTCTGG GTGGCCATTT CGGTGCTGCA GTTGGACGAG 
ATTACCCTGT ACGGCGCTGG ACTAGCTCTG CTTGAACAAA ATCTACACAC GCTTAAGTCA 
CAGGGCTGTT TTGACAAGAA GGAGACCATA GCCGAGGTCA TGATGAAGAC AAGGGAAAAG 
CTGGAGTGGC ATTTCAAGCA ACTGGATCAC GCCGTCGGCC TCTCCTTCCG CAGCAATTTC 
CACTTTGCCC TAGTGGGACA CTTGATTAAG GGCTTCCGCC ATCCTACACC TACAACCGTC 
TCACGCACCT CTCGTGTGCT GACGATGCTC TTGGGCATAA TAGCCAAACC CCTGCATCGG 
GACAAGTTCG AAGTAACACC TGACAGTGTG GCCTATTTGA CTGCGCTGGT GGCTGTCTCC 
GAGGAAGTCC GTTCCAGATG TCACGTAAAA CATGCCCTTC CCCGCTGGCC AGCCGATCTG 
AGTAGTAGTG TGGAAAATGG TGAAGCATCC GGCGGAGTGC AAGCTATCGG CCTGCCCCTG 
TCACGCCGTC AAAAAAGTTG GGACATCCTG GATCAGTCCG CCTTGCAGTT TGCCCGCCAA 
CACAAGGTTC CCACTCTTCA GAACGCGCGA GTTTTGTTCA AAACTCAACG CTCATTCTCG 
GTGCCGACGA CCAAAGATCC GAACAATGCC ACCGGCATCG AAGAACGTCA GGAACGCGGT 
TCGCGTTCGT CGGTGTCCAA CGAGTCCAAC GTACTGCTCG ATCCCGAGGT CTTACCTGAC 
CTGTCCATTC AAGCCCTGGT ACTGACTGTT TTGGCCACCT TGGTCAAGTA CTCTTCGGAT 
GAAGGCGAAA CGCGGGTTTT GTATCAGTAT TTGGCCGAAG GATCGGTGGT CTTTCCAAAG 
GTGTTTCCAG TTATCCATTC ACTGCTGGAC CAAAAGATAA ACAACATTTT GTCAGTGTCA 
CACGACCAAG TGGTGCTCAA CTCTGTGCAG AACATTATAC AAAATATGCT GGCCAGCGAA 
GATCCTTCCC AGCAGCAGCT GCACTTCCTG CAGAGTTGTG GATTTGGCGG ACTCTGGCGA 
TTTGCTGGTC CCTTCACCAA GTACAATATG ATGGGTGAGT CCTCGGAGCT GTTTGTCAAC 
TGTTTGGAGG CCATGGTTGA GACCTGTTTG CCAGGCGACG AATCCGCGCC GGTGCCACCA 
TCTCCGCGTC CATACAACCT GAGCTCCAGT CTGAGTAGCC TGACCCTGGG ATCACCCACG 
GATAAAGCAT TCTCATCGGA ATCATTAGAT TTTTATGACA ATTGCCCAGG CAGCGTCAGC 
AGCCTGCGAC GCGCCTCACA CAGCAAATCA CGCGCCAAAC ATCGAATTAA CGACAGTCCA 
TCACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733132.2
CDS	280..8688
Translation	MTQKPGEWASALLARFEDQLPNRIGAYGTQARMSQDQLVACLIHISRYRFSLVISGLTKMLQRVNEAALQNRHEPERCYFESLVIILTTLERCLTNQTKDTARFEEAMNVKLLLREISQFVDVQSDSNPNAAQLKALASKVLFALSQNHFSAVFNRISARIQELTSCSEENPDYNDIELIQHIDMDMIKLTKLLQETITKFRSKRAPPLILLYSLEKAIWNWIEYHPQEFQDLQRGTNRDISTCWEPLMDFVEYFKTENKKSKTLVWPLQMLLLILNPSCLEAVVNELQQSEKEKEKDKEKVASKSAQSTSRDKDFSAKQFIESIKRGLGQHSPSKQVTESAAIACVKLCKASTYINNTDSNNVVFKLVQFFINDLKALLFNPAKPFSRGQGYNFADIELMIDCWVSCFRINPHNIEALKVCLNLSSPQAYHFVIVCSLLRLAHIYVDFRLQNKNPFRIVNQPRLSWWPQTDVVHYRSAELRALFTDTLNKATQGYIAHTPLRYITSLTLKSKDTQKGLTRAEEGPAHKMLLLLLVRLIHADPTLLLNTQGKVAHEVQSSTLELINGLVSLVHQTTMPDVAQEAMEALLALHAPEKIEVWNPEAPINTFWDVSSQVLFSISQKLIQHQIANYTDVLKWLREILICRNTFLQRHKDYAHVGSQIAICKQAHIKMEVVFFMYLWSVDLDAVLTSLSCFGLLCEEAEICCSSDELTVGFIMPNYHIYQELAQLSTSATDSRICFFDNTHGNVLSRLTLQKRIMTLLRKIEHCVHGVQPAWEETFRNWEVSSKVLQTYPKCKGEDGQAEVFHRGMGKRRASHQSSEHDLEEQINEWANMTWFLLALGGVCLHKRSSSRQMLLQQSQNNASLGSLAQNSLYSSSTSSGHGSLHPSTVSLSTLPPAPPQDVSYCPVTQFVGQLLRLLVCSNEKIGLNIQKNVKELVGEEMSTQLYPILFDQVRAIVEKFFDQQGQVNVNVTDINTQFIEHTIYIMKSILDPKANKDPNNDQPSPSEHLGVTSIEGMMLGIVRYVRHLDMTVYAIRIKTKLCQLVEVMMKRRDDLAFRQEMKFRNKLVEYLTDWVMGTSHQIAPPSSADAAILTNTSLIFRDLDQACMEAVAALLRGLPLQPEESDRGDLMDAKSALFLKYFTLFMNLLNDCIDSSEAEKEMNNTPLLPPRPRMAAGKLTALRNATILAMSNLLGANIDSGLMHSIDLGYNPDLQTRAAFMEVLTQILQQGTEFDTLAETVLADRFEQLVQLVTMISDKGELPIAMALANVVTTSQMDELARVLVTLFDAKHLLSPLLWNMFYREVEVSDCMQTLFRGNSLGSKIMAFCFKIYGASYLQMLLEPLIRPLLDEEEETCFEVDPARLDPTEDIEQHRNNLIALTQKVFDAIINSSDRFPPQLRSMCHCLYQVLSKRFPNLLQNNIGAVGTVIFLRFINPAIVSPQELGIVDKQVHSSAKRGLMLMSKILQNIANHVEFSKEQHMLCFNDFLRDHFEAGRRFFIQIASDCETVDQTSHSMSFISDANVLALHRLLWTHQEKIGDYLSSSRDHKAVGRRPFDKMATLLAYLGPPEHKPVDSHMMFSSYARWSSIDMSSTNFEEIMVKHQMHEKEEFKTLKSMNIFYQAGTSKSGYPVFYYIARRYKIGETNGDLLIYHVILTLKPFCHSPFEVVIDFTHTCSDNRFRTEFLQKWFYVLPTVAYENVHAVYIYNCNSWVREYTKFHDRILAPLKGNRKLLFLESPNKLTDFIDAEQQKLPGATLSLDEDLKVFSNALKLSHKDTKVAIKVGPTALQITSAEKTKVLAHSVLLNDVYYASEIEEVCLVDDNQFTLSITNESGQLSFIHNDCDNIVQAIIHIRNRWELSQPDSVTVHQKIRPKDVPGTLLNMALLNLGSCDPNLRTAAYNLLCALTATFDLKIEGQLLETQGLCIPSNNTIFIKSVSEKLATNEPHLTLEFLEESIQGFQRSTIELKHLCLEYMTPWLKNLVKFCKSNDDSKKLKVSQILDKLINLTIDQKEMYPSVQAKIWGSIGQIPELIDMVLDNFLHKSITYGLGSPQVEIMADTAVALASANVQLVSKKVITRICRVMDKSCTNPTQYLEQHMMWDDIAILGRYLLMLSFNNCLDVATSVPYLFHTITFLVCSGSLSMRASTHGLVINIIHSLCTCTNPSFSEEAQRVLRLSLDEFSLPKFYLLFGISKVKSAAVTAFRSSCRHPTDKWLGNERVTQPLPADRERLSLPSLEVITDALLEIMEACMRDVPDCEWLNTWTSLARSFAFCYNPALQPRALIVYGCISKSVTDHEVKQLLRILVKALESFNDLILIEALVMCLTRIQPLLRPESPIHRALFWVAISVLQLDEITLYGAGLALLEQNLHTLKSQGCFDKKETIAEVMMKTREKLEWHFKQLDHAVGLSFRSNFHFALVGHLIKGFRHPTPTTVSRTSRVLTMLLGIIAKPLHRDKFEVTPDSVAYLTALVAVSEEVRSRCHVKHALPRWPADLSSSVENGEASGGVQAIGLPLSRRQKSWDILDQSALQFARQHKVPTLQNARVLFKTQRSFSVPTTKDPNNATGIEERQERGSRSSVSNESNVLLDPEVLPDLSIQALVLTVLATLVKYSSDEGETRVLYQYLAEGSVVFPKVFPVIHSLLDQKINNILSVSHDQVVLNSVQNIIQNMLASEDPSQQQLHFLQSCGFGGLWRFAGPFTKYNMMGESSELFVNCLEAMVETCLPGDESAPVPPSPRPYNLSSSLSSLTLGSPTDKAFSSESLDFYDNCPGSVSSLRRASHSKSRAKHRINDSPSH

Target ORF information:

RefSeq Version	NM_170253.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neurofibromin 1, transcript variant B (Nf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170253.2

ORF Insert Sequence:

ATGACCCAGA AGCCAGGCGA GTGGGCTAGC GCCCTTTTGG CACGTTTCGA GGATCAGCTG 
CCAAACAGAA TCGGTGCCTA TGGCACGCAG GCCAGGATGA GCCAGGACCA ATTGGTGGCC 
TGTCTGATCC ACATATCGCG CTACCGCTTC TCCCTTGTCA TATCCGGTCT CACCAAGATG 
CTGCAGAGGG TCAATGAGGC GGCCCTTCAA AATCGCCATG AGCCAGAGCG TTGCTACTTT 
GAGTCGCTGG TCATCATACT GACCACTCTG GAACGCTGTC TGACCAACCA GACCAAGGAC 
ACAGCACGTT TTGAAGAGGC CATGAATGTG AAGCTTCTGC TGCGCGAGAT CTCCCAGTTT 
GTCGACGTCC AGAGCGACAG CAATCCGAAT GCCGCCCAGC TCAAGGCCCT GGCATCCAAG 
GTGCTCTTTG CCTTGTCCCA GAATCACTTC TCGGCGGTGT TCAATCGCAT ATCGGCCAGG 
ATTCAAGAGC TGACATCCTG TTCCGAGGAA AACCCGGACT ACAACGACAT AGAATTAATT 
CAACACATCG ATATGGATAT GATAAAGCTA ACCAAGCTGC TACAAGAAAC CATCACAAAG 
TTTCGATCGA AGCGTGCTCC ACCTTTGATC TTGCTTTACT CGCTGGAGAA GGCTATTTGG 
AACTGGATCG AGTACCATCC ACAAGAGTTC CAGGACTTGC AACGAGGAAC CAATCGAGAT 
ATATCAACCT GCTGGGAACC CCTGATGGAC TTTGTAGAGT ACTTTAAAAC CGAAAACAAG 
AAAAGCAAGA CTCTTGTTTG GCCGCTTCAA ATGCTCTTGC TGATATTGAA TCCCTCTTGC 
CTTGAGGCTG TTGTTAACGA ACTTCAGCAG TCGGAGAAAG AGAAGGAAAA GGACAAGGAA 
AAGGTTGCCT CGAAGTCAGC GCAATCCACG TCCCGGGACA AGGACTTCTC GGCCAAGCAG 
TTCATCGAGA GTATCAAGAG AGGCTTGGGC CAGCACTCAC CATCCAAACA GGTGACCGAA 
TCTGCGGCAA TTGCCTGTGT AAAGCTGTGC AAAGCATCCA CCTACATCAA CAACACCGAC 
TCCAATAATG TGGTCTTCAA GTTGGTGCAA TTCTTTATCA ACGATCTCAA GGCACTGCTC 
TTTAATCCAG CAAAGCCCTT TTCGCGCGGT CAGGGGTACA ACTTCGCTGA TATCGAGCTG 
ATGATTGACT GCTGGGTGTC TTGTTTCCGG ATTAATCCTC ACAACATCGA GGCTCTAAAG 
GTTTGCCTGA ATTTGTCCTC GCCGCAGGCT TACCATTTTG TAATTGTGTG CTCTCTGCTA 
AGGTTGGCTC ACATATACGT TGACTTCCGT TTGCAGAACA AGAACCCCTT TCGAATAGTA 
AACCAACCGA GATTGTCTTG GTGGCCGCAA ACGGATGTGG TTCACTACCG TTCTGCTGAG 
CTACGAGCTT TGTTTACGGA TACGCTCAAC AAGGCCACCC AGGGTTATAT AGCCCATACG 
CCACTGCGCT ACATAACCTC CCTGACGCTC AAATCTAAAG ATACCCAGAA AGGTTTAACC 
CGCGCTGAAG AGGGTCCCGC CCACAAAATG CTATTACTGC TACTCGTACG GCTAATTCAT 
GCAGATCCCA CGCTTTTGTT GAATACCCAA GGAAAAGTGG CACATGAAGT GCAGAGCTCC 
ACATTGGAGC TCATCAACGG CTTAGTTAGT TTGGTGCATC AAACTACCAT GCCCGATGTG 
GCACAAGAGG CTATGGAGGC GCTGCTTGCT CTGCACGCCC CGGAGAAAAT AGAGGTTTGG 
AATCCAGAGG CTCCGATCAA TACGTTCTGG GACGTTAGCT CCCAAGTACT GTTTTCAATC 
TCGCAAAAGC TCATTCAACA CCAAATTGCC AATTACACTG ATGTCTTAAA GTGGCTACGC 
GAGATCCTCA TCTGTCGGAA TACTTTTCTT CAGCGACACA AGGATTATGC ACATGTGGGA 
AGTCAGATTG CCATTTGCAA GCAGGCTCAC ATTAAGATGG AAGTTGTTTT CTTCATGTAC 
CTGTGGAGTG TTGATTTAGA TGCGGTGCTG ACATCGCTAT CGTGTTTTGG ACTACTATGC 
GAGGAGGCTG AGATTTGCTG CAGTTCTGAT GAATTGACAG TCGGATTTAT TATGCCGAAT 
TACCACATTT ACCAGGAGCT GGCTCAGTTG TCCACATCTG CAACGGACTC TCGCATTTGC 
TTTTTTGACA ACACGCACGG CAATGTGCTG AGCCGTCTTA CACTTCAAAA GCGCATAATG 
ACCCTATTGC GCAAGATCGA GCACTGTGTT CATGGTGTTC AGCCCGCCTG GGAGGAAACC 
TTCCGCAACT GGGAAGTATC CAGCAAGGTT TTGCAAACGT ACCCCAAATG TAAAGGAGAA 
GATGGCCAAG CGGAGGTTTT CCATCGTGGC ATGGGCAAGC GGCGAGCGAG TCACCAAAGC 
TCAGAGCACG ATTTGGAGGA ACAAATTAAT GAATGGGCCA ATATGACATG GTTTCTATTA 
GCCTTGGGTG GCGTCTGCCT TCATAAACGT AGCAGCAGTC GTCAAATGCT GCTGCAGCAA 
TCACAGAACA ATGCTTCTTT GGGATCACTT GCTCAAAACT CTCTTTACTC AAGCTCCACA 
AGTTCTGGAC ATGGCTCTCT GCATCCTAGC ACGGTTTCAC TATCCACTCT TCCCCCAGCA 
CCGCCACAAG ATGTCAGCTA TTGTCCTGTA ACACAATTCG TGGGCCAACT ATTGCGCCTG 
TTAGTTTGCA GCAACGAAAA AATTGGACTT AATATTCAGA AAAATGTGAA GGAACTGGTG 
GGCGAGGAGA TGTCCACCCA ACTGTATCCT ATACTCTTCG ACCAGGTTAG AGCCATCGTG 
GAGAAGTTCT TCGATCAGCA AGGTCAGGTG AACGTCAATG TGACCGACAT CAACACGCAG 
TTTATCGAGC ACACCATCTA CATAATGAAA TCAATTCTGG ATCCCAAAGC CAACAAGGAT 
CCCAACAACG ATCAACCCTC GCCATCGGAA CATTTAGGTG TTACAAGCAT CGAAGGCATG 
ATGCTAGGAA TAGTGCGCTA CGTTCGCCAC CTGGATATGA CTGTTTATGC CATTCGAATC 
AAGACAAAAT TGTGCCAGCT TGTGGAAGTA ATGATGAAGC GACGCGACGA CCTCGCCTTT 
CGCCAGGAGA TGAAGTTCCG GAATAAACTG GTTGAATACT TGACCGACTG GGTGATGGGC 
ACCTCCCATC AGATTGCACC GCCCAGCTCG GCGGATGCCG CTATTCTTAC CAACACATCC 
CTCATATTTC GCGATCTAGA CCAAGCCTGC ATGGAGGCAG TGGCTGCCTT GCTTCGGGGC 
CTTCCCCTTC AGCCTGAGGA ATCGGATCGA GGCGATTTGA TGGATGCAAA GAGTGCGCTC 
TTTTTAAAGT ACTTTACCCT ATTTATGAAC CTGCTGAATG ATTGCATCGA TAGCTCCGAG 
GCGGAGAAGG AAATGAATAA TACCCCACTA TTGCCTCCGC GCCCTCGAAT GGCAGCTGGA 
AAACTGACCG CTCTGCGAAA TGCCACTATT CTAGCCATGT CTAATTTGTT GGGTGCCAAC 
ATTGACTCTG GTTTGATGCA CTCCATCGAT CTGGGTTATA ATCCTGATTT GCAAACGCGT 
GCCGCTTTCA TGGAGGTGTT GACTCAAATC CTGCAACAAG GCACCGAATT CGATACCTTG 
GCCGAAACTG TTTTAGCTGA TCGATTTGAG CAACTGGTCC AACTTGTTAC AATGATCAGC 
GACAAAGGAG AACTTCCCAT AGCAATGGCT TTGGCTAATG TGGTGACTAC ATCCCAAATG 
GACGAGCTGG CTAGGGTTCT GGTCACCCTA TTCGACGCCA AACACCTGTT ATCGCCACTC 
CTATGGAATA TGTTTTATCG CGAGGTGGAG GTCTCAGACT GCATGCAGAC ACTTTTCCGT 
GGAAATTCTT TGGGCAGCAA AATCATGGCC TTTTGCTTTA AAATATACGG CGCGAGCTAT 
CTCCAAATGC TACTAGAGCC TTTAATTCGT CCGCTTCTGG ATGAGGAGGA GGAGACCTGT 
TTTGAGGTGG ATCCAGCTCG TCTGGATCCA ACAGAAGATA TTGAACAGCA CAGGAACAAC 
TTGATTGCCC TAACGCAGAA GGTGTTTGAC GCTATTATCA ACTCGTCGGA TCGCTTTCCC 
CCGCAGCTGC GATCCATGTG CCATTGCCTG TACCAAGTGC TAAGCAAACG CTTCCCGAAT 
CTTCTTCAAA ACAATATCGG TGCTGTAGGC ACAGTCATCT TCTTGCGGTT CATCAATCCC 
GCCATAGTTT CCCCACAGGA ATTGGGAATC GTTGACAAAC AAGTGCACAG CTCGGCCAAA 
CGAGGTCTAA TGCTAATGTC CAAGATCCTT CAAAACATTG CTAACCACGT GGAGTTCTCC 
AAGGAGCAGC ACATGTTGTG TTTCAACGAT TTTCTGCGCG ATCACTTTGA AGCTGGGCGG 
CGATTCTTCA TACAGATTGC ATCAGACTGT GAGACCGTGG ATCAGACCTC GCACAGCATG 
AGTTTTATTT CAGATGCGAA CGTACTGGCA CTGCATCGGT TGCTGTGGAC GCATCAGGAG 
AAGATCGGCG ACTATCTGTC TAGCAGTCGA GACCACAAGG CGGTTGGAAG GCGGCCCTTC 
GACAAGATGG CTACTTTGCT AGCATACCTG GGACCACCGG AGCACAAGCC CGTGGATTCG 
CACATGATGT TCAGCTCGTA TGCACGCTGG AGCTCCATTG ACATGTCGTC GACAAACTTC 
GAGGAGATCA TGGTCAAGCA CCAAATGCAC GAGAAGGAGG AATTCAAGAC CCTCAAGTCG 
ATGAACATAT TCTACCAGGC CGGAACAAGC AAATCTGGCT ATCCTGTCTT TTACTATATA 
GCAAGACGAT ACAAGATTGG AGAAACGAAT GGAGACTTAC TTATATATCA CGTCATACTC 
ACTCTGAAAC CATTCTGCCA CTCGCCCTTC GAGGTGGTCA TCGATTTTAC GCACACCTGT 
TCAGATAATC GGTTCCGCAC CGAGTTTCTG CAGAAGTGGT TTTATGTCCT GCCCACGGTA 
GCCTACGAAA ATGTCCATGC TGTGTACATC TATAACTGTA ACTCGTGGGT GCGCGAATAT 
ACCAAGTTCC ACGATCGCAT TCTGGCACCA TTAAAGGGAA ATCGCAAACT TCTGTTCCTG 
GAGTCACCCA ACAAACTTAC TGATTTCATC GACGCGGAGC AACAGAAATT GCCTGGAGCA 
ACTCTTTCCT TGGACGAAGA TTTAAAGGTA TTCAGCAACG CGCTGAAGCT CAGCCATAAA 
GACACAAAGG TGGCTATCAA AGTGGGTCCC ACCGCATTGC AAATTACATC TGCCGAAAAG 
ACAAAGGTCT TGGCTCACTC CGTGCTCCTG AACGATGTAT ACTACGCCTC TGAAATTGAG 
GAGGTGTGCT TGGTGGACGA TAACCAGTTC ACCCTGTCTA TAACTAACGA AAGTGGCCAG 
CTTAGCTTTA TTCACAACGA TTGCGACAAC ATTGTTCAAG CCATCATACA CATCCGAAAT 
CGATGGGAGC TTAGTCAGCC GGACTCCGTG ACGGTTCACC AAAAGATCCG ACCAAAAGAT 
GTGCCTGGAA CGCTGCTGAA CATGGCGCTA CTCAATCTGG GATCATGTGA CCCCAATCTA 
AGGACTGCTG CCTACAATTT GCTGTGTGCT TTGACCGCTA CTTTTGATCT AAAGATTGAG 
GGTCAACTGT TGGAGACCCA AGGACTTTGC ATACCCTCAA ATAATACCAT ATTTATCAAA 
TCCGTTAGCG AAAAGCTGGC CACCAATGAA CCGCATCTGA CTCTGGAATT CCTTGAGGAG 
TCCATTCAGG GCTTCCAGCG CAGCACCATA GAGCTGAAAC ATTTGTGTTT GGAGTACATG 
ACGCCATGGT TAAAGAACCT AGTCAAATTT TGCAAGTCCA ACGATGACTC CAAAAAGCTT 
AAGGTCTCCC AAATTCTGGA CAAGCTCATC AATTTAACTA TTGACCAAAA GGAAATGTAT 
CCCTCAGTGC AGGCCAAGAT CTGGGGATCA ATTGGACAGA TCCCCGAGCT GATCGACATG 
GTTTTGGATA ACTTTTTGCA CAAATCGATC ACATATGGCT TGGGATCACC ACAGGTGGAG 
ATTATGGCTG ACACGGCAGT GGCTCTCGCT TCAGCGAATG TTCAGCTGGT GTCCAAAAAG 
GTCATAACGC GAATATGCCG AGTCATGGAC AAATCCTGCA CAAATCCCAC ACAATATCTG 
GAGCAGCATA TGATGTGGGA CGATATTGCA ATTCTTGGTC GATACCTGCT CATGTTGTCG 
TTCAACAATT GTTTGGATGT GGCCACATCG GTGCCATATC TATTCCACAC CATTACGTTT 
TTGGTATGTT CAGGATCCCT GTCGATGCGA GCCTCTACTC ATGGCCTAGT GATCAACATC 
ATCCACTCGC TGTGCACATG CACAAATCCC TCATTTTCAG AGGAGGCGCA GCGAGTACTC 
CGACTGTCCC TGGATGAATT CTCACTGCCC AAGTTCTATC TACTCTTCGG CATCAGCAAG 
GTCAAGTCCG CAGCAGTTAC CGCTTTCCGC TCCAGCTGCC GTCACCCTAC AGATAAATGG 
CTAGGAAATG AAAGGGTTAC CCAGCCTCTG CCCGCCGATC GAGAGCGTTT ATCCCTACCA 
TCGCTTGAGG TTATCACGGA TGCCCTGCTG GAAATCATGG AGGCTTGCAT GCGAGATGTT 
CCGGACTGCG AATGGCTCAA CACCTGGACC TCTTTGGCTC GCAGTTTTGC TTTCTGTTAC 
AATCCAGCGC TACAGCCTAG AGCCCTTATA GTTTACGGGT GCATCAGCAA GAGTGTTACC 
GATCACGAGG TCAAGCAGTT ACTACGCATC CTGGTTAAGG CCCTCGAATC TTTCAACGAT 
CTCATTCTGA TCGAGGCTCT AGTAATGTGC TTAACTCGCA TTCAACCACT ACTCCGACCA 
GAGTCGCCCA TTCATCGGGC CCTCTTCTGG GTGGCCATTT CGGTGCTGCA GTTGGACGAG 
ATTACCCTGT ACGGCGCTGG ACTAGCTCTG CTTGAACAAA ATCTACACAC GCTTAAGTCA 
CAGGGCTGTT TTGACAAGAA GGAGACCATA GCCGAGGTCA TGATGAAGAC AAGGGAAAAG 
CTGGAGTGGC ATTTCAAGCA ACTGGATCAC GCCGTCGGCC TCTCCTTCCG CAGCAATTTC 
CACTTTGCCC TAGTGGGACA CTTGATTAAG GGCTTCCGCC ATCCTACACC TACAACCGTC 
TCACGCACCT CTCGTGTGCT GACGATGCTC TTGGGCATAA TAGCCAAACC CCTGCATCGG 
GACAAGTTCG AAGTAACACC TGACAGTGTG GCCTATTTGA CTGCGCTGGT GGCTGTCTCC 
GAGGAAGTCC GTTCCAGATG TCACGTAAAA CATGCCCTTC CCCGCTGGCC AGCCGATCTG 
AGTAGTAGTG TGGAAAATGG TGAAGCATCC GGCGGAGTGC AAGCTATCGG CCTGCCCCTG 
TCACGCCGTC AAAAAAGTTG GGACATCCTG GATCAGTCCG CCTTGCAGTT TGCCCGCCAA 
CACAAGGTTC CCACTCTTCA GAACGCGCGA GTTTTGTTCA AAACTCAACG CTCATTCTCG 
GTGCCGACGA CCAAAGATCC GAACAATGCC ACCGGCATCG AAGAACGTCA GGAACGCGGT 
TCGCGTTCGT CGGTGTCCAA CGAGTCCAAC GTACTGCTCG ATCCCGAGGT CTTACCTGAC 
CTGTCCATTC AAGCCCTGGT ACTGACTGTT TTGGCCACCT TGGTCAAGTA CTCTTCGGAT 
GAAGGCGAAA CGCGGGTTTT GTATCAGTAT TTGGCCGAAG GATCGGTGGT CTTTCCAAAG 
GTGTTTCCAG TTATCCATTC ACTGCTGGAC CAAAAGATAA ACAACATTTT GTCAGTGTCA 
CACGACCAAG TGGTGCTCAA CTCTGTGCAG AACATTATAC AAAATATGCT GGCCAGCGAA 
GATCCTTCCC AGCAGCAGCT GCACTTCCTG CAGAGTTGTG GATTTGGCGG ACTCTGGCGA 
TTTGCTGGTC CCTTCACCAA GTACAATATG ATGGGTGAGT CCTCGGAGCT GTTTGTCAAC 
TGTTTGGAGG CCATGGTTGA GACCTGTTTG CCAGGCGACG AATCCGCGCC GGTGCCACCA 
TCTCCGCGTC CATACAACCT GAGCTCCAGT CTGAGTAGCC TGACCCTGGG ATCACCCACG 
GATAAAGCAT TCTCATCGGA ATCATTAGAT TTTTATGACA ATTGCCCAGG CAGCGTCAGC 
AGCCTGCGAC GCGCCTCACA CAGCAAATCA CGCGCCAAAC ATCGAATTAA CGACAGTCCA 
TCACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nf1 ( NM_001276049.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262978.1 Drosophila melanogaster neurofibromin 1, transcript variant E (Nf1), mRNA.

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CloneID	OFa08849
Clone ID Related Accession (Same CDS sequence)	NM_001276049.1
Accession Version	NM_001276049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neurofibromin 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCCAGA AGCCAGGCGA GTGGGCTAGC GCCCTTTTGG CACGTTTCGA GGATCAGCTG 
CCAAACAGAA TCGGTGCCTA TGGCACGCAG GCCAGGATGA GCCAGGACCA ATTGGTGGCC 
TGTCTGATCC ACATATCGCG CTACCGCTTC TCCCTTGTCA TATCCGGTCT CACCAAGATG 
CTGCAGAGGG TCAATGAGGC GGCCCTTCAA AATCGCCATG AGCCAGAGCG TTGCTACTTT 
GAGTCGCTGG TCATCATACT GACCACTCTG GAACGCTGTC TGACCAACCA GACCAAGGAC 
ACAGCACGTT TTGAAGAGGC CATGAATGTG AAGCTTCTGC TGCGCGAGAT CTCCCAGTTT 
GTCGACGTCC AGAGCGACAG CAATCCGAAT GCCGCCCAGC TCAAGGCCCT GGCATCCAAG 
GTGCTCTTTG CCTTGTCCCA GAATCACTTC TCGGCGGTGT TCAATCGCAT ATCGGCCAGG 
ATTCAAGAGC TGACATCCTG TTCCGAGGAA AACCCGGACT ACAACGACAT AGAATTAATT 
CAACACATCG ATATGGATAT GATAAAGCTA ACCAAGCTGC TACAAGAAAC CATCACAAAG 
TTTCGATCGA AGCGTGCTCC ACCTTTGATC TTGCTTTACT CGCTGGAGAA GGCTATTTGG 
AACTGGATCG AGTACCATCC ACAAGAGTTC CAGGACTTGC AACGAGGAAC CAATCGAGAT 
ATATCAACCT GCTGGGAACC CCTGATGGAC TTTGTAGAGT ACTTTAAAAC CGAAAACAAG 
AAAAGCAAGA CTCTTGTTTG GCCGCTTCAA ATGCTCTTGC TGATATTGAA TCCCTCTTGC 
CTTGAGGCTG TTGTTAACGA ACTTCAGCAG TCGGAGAAAG AGAAGGAAAA GGACAAGGAA 
AAGGTTGCCT CGAAGTCAGC GCAATCCACG TCCCGGGACA AGGACTTCTC GGCCAAGCAG 
TTCATCGAGA GTATCAAGAG AGGCTTGGGC CAGCACTCAC CATCCAAACA GGTGACCGAA 
TCTGCGGCAA TTGCCTGTGT AAAGCTGTGC AAAGCATCCA CCTACATCAA CAACACCGAC 
TCCAATAATG TGGTCTTCAA GTTGGTGCAA TTCTTTATCA ACGATCTCAA GGCACTGCTC 
TTTAATCCAG CAAAGCCCTT TTCGCGCGGT CAGGGGTACA ACTTCGCTGA TATCGAGCTG 
ATGATTGACT GCTGGGTGTC TTGTTTCCGG ATTAATCCTC ACAACATCGA GGCTCTAAAG 
GTTTGCCTGA ATTTGTCCTC GCCGCAGGCT TACCATTTTG TAATTGTGTG CTCTCTGCTA 
AGGTTGGCTC ACATATACGT TGACTTCCGT TTGCAGAACA AGAACCCCTT TCGAATAGTA 
AACCAACCGA GATTGTCTTG GTGGCCGCAA ACGGATGTGG TTCACTACCG TTCTGCTGAG 
CTACGAGCTT TGTTTACGGA TACGCTCAAC AAGGCCACCC AGGGTTATAT AGCCCATACG 
CCACTGCGCT ACATAACCTC CCTGACGCTC AAATCTAAAG ATACCCAGAA AGGTTTAACC 
CGCGCTGAAG AGGGTCCCGC CCACAAAATG CTATTACTGC TACTCGTACG GCTAATTCAT 
GCAGATCCCA CGCTTTTGTT GAATACCCAA GGAAAAGTGG CACATGAAGT GCAGAGCTCC 
ACATTGGAGC TCATCAACGG CTTAGTTAGT TTGGTGCATC AAACTACCAT GCCCGATGTG 
GCACAAGAGG CTATGGAGGC GCTGCTTGCT CTGCACGCCC CGGAGAAAAT AGAGGTTTGG 
AATCCAGAGG CTCCGATCAA TACGTTCTGG GACGTTAGCT CCCAAGTACT GTTTTCAATC 
TCGCAAAAGC TCATTCAACA CCAAATTGCC AATTACACTG ATGTCTTAAA GTGGCTACGC 
GAGATCCTCA TCTGTCGGAA TACTTTTCTT CAGCGACACA AGGATTATGC ACATGTGGGA 
AGTCAGATTG CCATTTGCAA GCAGGCTCAC ATTAAGATGG AAGTTGTTTT CTTCATGTAC 
CTGTGGAGTG TTGATTTAGA TGCGGTGCTG ACATCGCTAT CGTGTTTTGG ACTACTATGC 
GAGGAGGCTG AGATTTGCTG CAGTTCTGAT GAATTGACAG TCGGATTTAT TATGCCGAAT 
TACCACATTT ACCAGGAGCT GGCTCAGTTG TCCACATCTG CAACGGACTC TCGCATTTGC 
TTTTTTGACA ACACGCACGG CAATGTGCTG AGCCGTCTTA CACTTCAAAA GCGCATAATG 
ACCCTATTGC GCAAGATCGA GCACTGTGTT CATGGTGTTC AGCCCGCCTG GGAGGAAACC 
TTCCGCAACT GGGAAGTATC CAGCAAGGTT TTGCAAACGT ACCCCAAATG TAAAGGAGAA 
GATGGCCAAG CGGAGGTTTT CCATCGTGGC ATGGGCAAGC GGCGAGCGAG TCACCAAAGC 
TCAGAGCACG ATTTGGAGGA ACAAATTAAT GAATGGGCCA ATATGACATG GTTTCTATTA 
GCCTTGGGTG GCGTCTGCCT TCATAAACGT AGCAGCAGTC GTCAAATGCT GCTGCAGCAA 
TCACAGAACA ATGCTTCTTT GGGATCACTT GCTCAAAACT CTCTTTACTC AAGCTCCACA 
AGTTCTGGAC ATGGCTCTCT GCATCCTAGC ACGGTTTCAC TATCCACTCT TCCCCCAGCA 
CCGCCACAAG ATGTCAGCTA TTGTCCTGTA ACACAATTCG TGGGCCAACT ATTGCGCCTG 
TTAGTTTGCA GCAACGAAAA AATTGGACTT AATATTCAGA AAAATGTGAA GGAACTGGTG 
GGCGAGGAGA TGTCCACCCA ACTGTATCCT ATACTCTTCG ACCAGGTTAG AGCCATCGTG 
GAGAAGTTCT TCGATCAGCA AGGTCAGGTG AACGTCAATG TGACCGACAT CAACACGCAG 
TTTATCGAGC ACACCATCTA CATAATGAAA TCAATTCTGG ATCCCAAAGC CAACAAGGAT 
CCCAACAACG ATCAACCCTC GCCATCGGAA CATTTAGGTG TTACAAGCAT CGAAGGCATG 
ATGCTAGGAA TAGTGCGCTA CGTTCGCCAC CTGGATATGA CTGTTTATGC CATTCGAATC 
AAGACAAAAT TGTGCCAGCT TGTGGAAGTA ATGATGAAGC GACGCGACGA CCTCGCCTTT 
CGCCAGGAGA TGAAGTTCCG GAATAAACTG GTTGAATACT TGACCGACTG GGTGATGGGC 
ACCTCCCATC AGATTGCACC GCCCAGCTCG GCGGATGCCG CTATTCTTAC CAACACATCC 
CTCATATTTC GCGATCTAGA CCAAGCCTGC ATGGAGGCAG TGGCTGCCTT GCTTCGGGGC 
CTTCCCCTTC AGCCTGAGGA ATCGGATCGA GGCGATTTGA TGGATGCAAA GAGTGCGCTC 
TTTTTAAAGT ACTTTACCCT ATTTATGAAC CTGCTGAATG ATTGCATCGA TAGCTCCGAG 
GCGGAGAAGG AAATGAATAA TACCCCACTA TTGCCTCCGC GCCCTCGAAT GGCAGCTGGA 
AAACTGACCG CTCTGCGAAA TGCCACTATT CTAGCCATGT CTAATTTGTT GGGTGCCAAC 
ATTGACTCTG GTTTGATGCA CTCCATCGAT CTGGGTTATA ATCCTGATTT GCAAACGCGT 
GCCGCTTTCA TGGAGGTGTT GACTCAAATC CTGCAACAAG GCACCGAATT CGATACCTTG 
GCCGAAACTG TTTTAGCTGA TCGATTTGAG CAACTGGTCC AACTTGTTAC AATGATCAGC 
GACAAAGGAG AACTTCCCAT AGCAATGGCT TTGGCTAATG TGGTGACTAC ATCCCAAATG 
GACGAGCTGG CTAGGGTTCT GGTCACCCTA TTCGACGCCA AACACCTGTT ATCGCCACTC 
CTATGGAATA TGTTTTATCG CGAGGTGGAG GTCTCAGACT GCATGCAGAC ACTTTTCCGT 
GGAAATTCTT TGGGCAGCAA AATCATGGCC TTTTGCTTTA AAATATACGG CGCGAGCTAT 
CTCCAAATGC TACTAGAGCC TTTAATTCGT CCGCTTCTGG ATGAGGAGGA GGAGACCTGT 
TTTGAGGTGG ATCCAGCTCG TCTGGATCCA ACAGAAGATA TTGAACAGCA CAGGAACAAC 
TTGATTGCCC TAACGCAGAA GGTGTTTGAC GCTATTATCA ACTCGTCGGA TCGCTTTCCC 
CCGCAGCTGC GATCCATGTG CCATTGCCTG TACCAAGTGC TAAGCAAACG CTTCCCGAAT 
CTTCTTCAAA ACAATATCGG TGCTGTAGGC ACAGTCATCT TCTTGCGGTT CATCAATCCC 
GCCATAGTTT CCCCACAGGA ATTGGGAATC GTTGACAAAC AAGTGCACAG CTCGGCCAAA 
CGAGGTCTAA TGCTAATGTC CAAGATCCTT CAAAACATTG CTAACCACGT GGAGTTCTCC 
AAGGAGCAGC ACATGTTGTG TTTCAACGAT TTTCTGCGCG ATCACTTTGA AGCTGGGCGG 
CGATTCTTCA TACAGATTGC ATCAGACTGT GAGACCGTGG ATCAGACCTC GCACAGCATG 
AGTTTTATTT CAGATGCGAA CGTACTGGCA CTGCATCGGT TGCTGTGGAC GCATCAGGAG 
AAGATCGGCG ACTATCTGTC TAGCAGTCGA GACCACAAGG CGGTTGGAAG GCGGCCCTTC 
GACAAGATGG CTACTTTGCT AGCATACCTG GGACCACCGG AGCACAAGCC CGTGGATTCG 
CACATGATGT TCAGCTCGTA TGCACGCTGG AGCTCCATTG ACATGTCGTC GACAAACTTC 
GAGGAGATCA TGGTCAAGCA CCAAATGCAC GAGAAGGAGG AATTCAAGAC CCTCAAGTCG 
ATGAACATAT TCTACCAGGC CGGAACAAGC AAATCTGGCT ATCCTGTCTT TTACTATATA 
GCAAGACGAT ACAAGATTGG AGAAACGAAT GGAGACTTAC TTATATATCA CGTCATACTC 
ACTCTGAAAC CATTCTGCCA CTCGCCCTTC GAGGTGGTCA TCGATTTTAC GCACACCTGT 
TCAGATAATC GGTTCCGCAC CGAGTTTCTG CAGAAGTGGT TTTATGTCCT GCCCACGGTA 
GCCTACGAAA ATGTCCATGC TGTGTACATC TATAACTGTA ACTCGTGGGT GCGCGAATAT 
ACCAAGTTCC ACGATCGCAT TCTGGCACCA TTAAAGGGAA ATCGCAAACT TCTGTTCCTG 
GAGTCACCCA ACAAACTTAC TGATTTCATC GACGCGGAGC AACAGAAATT GCCTGGAGCA 
ACTCTTTCCT TGGACGAAGA TTTAAAGGTA TTCAGCAACG CGCTGAAGCT CAGCCATAAA 
GACACAAAGG TGGCTATCAA AGTGGGTCCC ACCGCATTGC AAATTACATC TGCCGAAAAG 
ACAAAGGTCT TGGCTCACTC CGTGCTCCTG AACGATGTAT ACTACGCCTC TGAAATTGAG 
GAGGTGTGCT TGGTGGACGA TAACCAGTTC ACCCTGTCTA TAACTAACGA AAGTGGCCAG 
CTTAGCTTTA TTCACAACGA TTGCGACAAC ATTGTTCAAG CCATCATACA CATCCGAAAT 
CGATGGGAGC TTAGTCAGCC GGACTCCGTG ACGGTTCACC AAAAGATCCG ACCAAAAGAT 
GTGCCTGGAA CGCTGCTGAA CATGGCGCTA CTCAATCTGG GATCATGTGA CCCCAATCTA 
AGGACTGCTG CCTACAATTT GCTGTGTGCT TTGACCGCTA CTTTTGATCT AAAGATTGAG 
GGTCAACTGT TGGAGACCCA AGGACTTTGC ATACCCTCAA ATAATACCAT ATTTATCAAA 
TCCGTTAGCG AAAAGCTGGC CACCAATGAA CCGCATCTGA CTCTGGAATT CCTTGAGGAG 
TCCATTCAGG GCTTCCAGCG CAGCACCATA GAGCTGAAAC ATTTGTGTTT GGAGTACATG 
ACGCCATGGT TAAAGAACCT AGTCAAATTT TGCAAGTCCA ACGATGACTC CAAAAAGCTT 
AAGGTCTCCC AAATTCTGGA CAAGCTCATC AATTTAACTA TTGACCAAAA GGAAATGTAT 
CCCTCAGTGC AGGCCAAGAT CTGGGGATCA ATTGGACAGA TCCCCGAGCT GATCGACATG 
GTTTTGGATA ACTTTTTGCA CAAATCGATC ACATATGGCT TGGGATCACC ACAGGTGGAG 
ATTATGGCTG ACACGGCAGT GGCTCTCGCT TCAGCGAATG TTCAGCTGGT GTCCAAAAAG 
GTCATAACGC GAATATGCCG AGTCATGGAC AAATCCTGCA CAAATCCCAC ACAATATCTG 
GAGCAGCATA TGATGTGGGA CGATATTGCA ATTCTTGGTC GATACCTGCT CATGTTGTCG 
TTCAACAATT GTTTGGATGT GGCCACATCG GTGCCATATC TATTCCACAC CATTACGTTT 
TTGGTATGTT CAGGATCCCT GTCGATGCGA GCCTCTACTC ATGGCCTAGT GATCAACATC 
ATCCACTCGC TGTGCACATG CACAAATCCC TCATTTTCAG AGGAGGCGCA GCGAGTACTC 
CGACTGTCCC TGGATGAATT CTCACTGCCC AAGTTCTATC TACTCTTCGG CATCAGCAAG 
GTCAAGTCCG CAGCAGTTAC CGCTTTCCGC TCCAGCTGCC GTCACCCTAC AGATAAATGG 
CTAGGAAATG AAAGGGTTAC CCAGCCTCTG CCCGCCGATC GAGAGCGTTT ATCCCTACCA 
TCGCTTGAGG TTATCACGGA TGCCCTGCTG GAAATCATGG AGGCTTGCAT GCGAGATGTT 
CCGGACTGCG AATGGCTCAA CACCTGGACC TCTTTGGCTC GCAGTTTTGC TTTCTGTTAC 
AATCCAGCGC TACAGCCTAG AGCCCTTATA GTTTACGGGT GCATCAGCAA GAGTGTTACC 
GATCACGAGG TCAAGCAGTT ACTACGCATC CTGGTTAAGG CCCTCGAATC TTTCAACGAT 
CTCATTCTGA TCGAGGCTCT AGTAATGTGC TTAACTCGCA TTCAACCACT ACTCCGACCA 
GAGTCGCCCA TTCATCGGGC CCTCTTCTGG GTGGCCATTT CGGTGCTGCA GTTGGACGAG 
ATTACCCTGT ACGGCGCTGG ACTAGCTCTG CTTGAACAAA ATCTACACAC GCTTAAGTCA 
CAGGGCTGTT TTGACAAGAA GGAGACCATA GCCGAGGTCA TGATGAAGAC AAGGGAAAAG 
CTGGAGTGGC ATTTCAAGCA ACTGGATCAC GCCGTCGGCC TCTCCTTCCG CAGCAATTTC 
CACTTTGCCC TAGTGGGACA CTTGATTAAG GGCTTCCGCC ATCCTACACC TACAACCGTC 
TCACGCACCT CTCGTGTGCT GACGATGCTC TTGGGCATAA TAGCCAAACC CCTGCATCGG 
GACAAGTTCG AAGTAACACC TGACAGTGTG GCCTATTTGA CTGCGCTGGT GGCTGTCTCC 
GAGGAAGTCC GTTCCAGATG TCACGTAAAA CATGCCCTTC CCCGCTGGCC AGCCGATCTG 
AGTAGTAGTG TGGAAAATGG TGAAGCATCC GGCGGAGTGC AAGCTATCGG CCTGCCCCTG 
TCACGCCGTC AAAAAAGTTG GGACATCCTG GATCAGTCCG CCTTGCAGTT TGCCCGCCAA 
CACAAGGTTC CCACTCTTCA GAACGCGCGA GTTTTGTTCA AAACTCAACG CTCATTCTCG 
GTGCCGACGA CCAAAGATCC GAACAATGCC ACCGGCATCG AAGAACGTCA GGAACGCGGT 
TCGCGTTCGT CGGTGTCCAA CGAGTCCAAC GTACTGCTCG ATCCCGAGGT CTTACCTGAC 
CTGTCCATTC AAGCCCTGGT ACTGACTGTT TTGGCCACCT TGGTCAAGTA CTCTTCGGAT 
GAAGGCGAAA CGCGGGTTTT GTATCAGTAT TTGGCCGAAG GATCGGTGGT CTTTCCAAAG 
GTGTTTCCAG TTATCCATTC ACTGCTGGAC CAAAAGATAA ACAACATTTT GTCAGTGTCA 
CACGACCAAG TGGTGCTCAA CTCTGTGCAG AACATTATAC AAAATATGCT GGCCAGCGAA 
GATCCTTCCC AGCAGCAGCT GCACTTCCTG CAGAGTTGTG GATTTGGCGG ACTCTGGCGA 
TTTGCTGGTC CCTTCACCAA GTACAATATG ATGGGTGAGT CCTCGGAGCT GTTTGTCAAC 
TGTTTGGAGG CCATGGTTGA GACCTGTTTG CCAGGCGACG AATCCGCGCC GGTGCCACCA 
TCTCCGCGTC CATACAACCT GAGCTCCAGT CTGAGTAGCC TGACCCTGGG ATCACCCACG 
GATAAAGCTG CATGAATAAC AACTACAAAC CCGCGCACTA GACGTACCAC ACTTATCGCA 
AAAGTAATGC GTTTCCTAAA TCTGCTTAAA AAGTGCTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262978.1
CDS	280..8661
Translation	MTQKPGEWASALLARFEDQLPNRIGAYGTQARMSQDQLVACLIHISRYRFSLVISGLTKMLQRVNEAALQNRHEPERCYFESLVIILTTLERCLTNQTKDTARFEEAMNVKLLLREISQFVDVQSDSNPNAAQLKALASKVLFALSQNHFSAVFNRISARIQELTSCSEENPDYNDIELIQHIDMDMIKLTKLLQETITKFRSKRAPPLILLYSLEKAIWNWIEYHPQEFQDLQRGTNRDISTCWEPLMDFVEYFKTENKKSKTLVWPLQMLLLILNPSCLEAVVNELQQSEKEKEKDKEKVASKSAQSTSRDKDFSAKQFIESIKRGLGQHSPSKQVTESAAIACVKLCKASTYINNTDSNNVVFKLVQFFINDLKALLFNPAKPFSRGQGYNFADIELMIDCWVSCFRINPHNIEALKVCLNLSSPQAYHFVIVCSLLRLAHIYVDFRLQNKNPFRIVNQPRLSWWPQTDVVHYRSAELRALFTDTLNKATQGYIAHTPLRYITSLTLKSKDTQKGLTRAEEGPAHKMLLLLLVRLIHADPTLLLNTQGKVAHEVQSSTLELINGLVSLVHQTTMPDVAQEAMEALLALHAPEKIEVWNPEAPINTFWDVSSQVLFSISQKLIQHQIANYTDVLKWLREILICRNTFLQRHKDYAHVGSQIAICKQAHIKMEVVFFMYLWSVDLDAVLTSLSCFGLLCEEAEICCSSDELTVGFIMPNYHIYQELAQLSTSATDSRICFFDNTHGNVLSRLTLQKRIMTLLRKIEHCVHGVQPAWEETFRNWEVSSKVLQTYPKCKGEDGQAEVFHRGMGKRRASHQSSEHDLEEQINEWANMTWFLLALGGVCLHKRSSSRQMLLQQSQNNASLGSLAQNSLYSSSTSSGHGSLHPSTVSLSTLPPAPPQDVSYCPVTQFVGQLLRLLVCSNEKIGLNIQKNVKELVGEEMSTQLYPILFDQVRAIVEKFFDQQGQVNVNVTDINTQFIEHTIYIMKSILDPKANKDPNNDQPSPSEHLGVTSIEGMMLGIVRYVRHLDMTVYAIRIKTKLCQLVEVMMKRRDDLAFRQEMKFRNKLVEYLTDWVMGTSHQIAPPSSADAAILTNTSLIFRDLDQACMEAVAALLRGLPLQPEESDRGDLMDAKSALFLKYFTLFMNLLNDCIDSSEAEKEMNNTPLLPPRPRMAAGKLTALRNATILAMSNLLGANIDSGLMHSIDLGYNPDLQTRAAFMEVLTQILQQGTEFDTLAETVLADRFEQLVQLVTMISDKGELPIAMALANVVTTSQMDELARVLVTLFDAKHLLSPLLWNMFYREVEVSDCMQTLFRGNSLGSKIMAFCFKIYGASYLQMLLEPLIRPLLDEEEETCFEVDPARLDPTEDIEQHRNNLIALTQKVFDAIINSSDRFPPQLRSMCHCLYQVLSKRFPNLLQNNIGAVGTVIFLRFINPAIVSPQELGIVDKQVHSSAKRGLMLMSKILQNIANHVEFSKEQHMLCFNDFLRDHFEAGRRFFIQIASDCETVDQTSHSMSFISDANVLALHRLLWTHQEKIGDYLSSSRDHKAVGRRPFDKMATLLAYLGPPEHKPVDSHMMFSSYARWSSIDMSSTNFEEIMVKHQMHEKEEFKTLKSMNIFYQAGTSKSGYPVFYYIARRYKIGETNGDLLIYHVILTLKPFCHSPFEVVIDFTHTCSDNRFRTEFLQKWFYVLPTVAYENVHAVYIYNCNSWVREYTKFHDRILAPLKGNRKLLFLESPNKLTDFIDAEQQKLPGATLSLDEDLKVFSNALKLSHKDTKVAIKVGPTALQITSAEKTKVLAHSVLLNDVYYASEIEEVCLVDDNQFTLSITNESGQLSFIHNDCDNIVQAIIHIRNRWELSQPDSVTVHQKIRPKDVPGTLLNMALLNLGSCDPNLRTAAYNLLCALTATFDLKIEGQLLETQGLCIPSNNTIFIKSVSEKLATNEPHLTLEFLEESIQGFQRSTIELKHLCLEYMTPWLKNLVKFCKSNDDSKKLKVSQILDKLINLTIDQKEMYPSVQAKIWGSIGQIPELIDMVLDNFLHKSITYGLGSPQVEIMADTAVALASANVQLVSKKVITRICRVMDKSCTNPTQYLEQHMMWDDIAILGRYLLMLSFNNCLDVATSVPYLFHTITFLVCSGSLSMRASTHGLVINIIHSLCTCTNPSFSEEAQRVLRLSLDEFSLPKFYLLFGISKVKSAAVTAFRSSCRHPTDKWLGNERVTQPLPADRERLSLPSLEVITDALLEIMEACMRDVPDCEWLNTWTSLARSFAFCYNPALQPRALIVYGCISKSVTDHEVKQLLRILVKALESFNDLILIEALVMCLTRIQPLLRPESPIHRALFWVAISVLQLDEITLYGAGLALLEQNLHTLKSQGCFDKKETIAEVMMKTREKLEWHFKQLDHAVGLSFRSNFHFALVGHLIKGFRHPTPTTVSRTSRVLTMLLGIIAKPLHRDKFEVTPDSVAYLTALVAVSEEVRSRCHVKHALPRWPADLSSSVENGEASGGVQAIGLPLSRRQKSWDILDQSALQFARQHKVPTLQNARVLFKTQRSFSVPTTKDPNNATGIEERQERGSRSSVSNESNVLLDPEVLPDLSIQALVLTVLATLVKYSSDEGETRVLYQYLAEGSVVFPKVFPVIHSLLDQKINNILSVSHDQVVLNSVQNIIQNMLASEDPSQQQLHFLQSCGFGGLWRFAGPFTKYNMMGESSELFVNCLEAMVETCLPGDESAPVPPSPRPYNLSSSLSSLTLGSPTDKAA*ITTTNPRTRRTTLIAKVMRFLNLLKKCF

Target ORF information:

RefSeq Version	NM_001276049.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neurofibromin 1, transcript variant E (Nf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276049.1

ORF Insert Sequence:

ATGACCCAGA AGCCAGGCGA GTGGGCTAGC GCCCTTTTGG CACGTTTCGA GGATCAGCTG 
CCAAACAGAA TCGGTGCCTA TGGCACGCAG GCCAGGATGA GCCAGGACCA ATTGGTGGCC 
TGTCTGATCC ACATATCGCG CTACCGCTTC TCCCTTGTCA TATCCGGTCT CACCAAGATG 
CTGCAGAGGG TCAATGAGGC GGCCCTTCAA AATCGCCATG AGCCAGAGCG TTGCTACTTT 
GAGTCGCTGG TCATCATACT GACCACTCTG GAACGCTGTC TGACCAACCA GACCAAGGAC 
ACAGCACGTT TTGAAGAGGC CATGAATGTG AAGCTTCTGC TGCGCGAGAT CTCCCAGTTT 
GTCGACGTCC AGAGCGACAG CAATCCGAAT GCCGCCCAGC TCAAGGCCCT GGCATCCAAG 
GTGCTCTTTG CCTTGTCCCA GAATCACTTC TCGGCGGTGT TCAATCGCAT ATCGGCCAGG 
ATTCAAGAGC TGACATCCTG TTCCGAGGAA AACCCGGACT ACAACGACAT AGAATTAATT 
CAACACATCG ATATGGATAT GATAAAGCTA ACCAAGCTGC TACAAGAAAC CATCACAAAG 
TTTCGATCGA AGCGTGCTCC ACCTTTGATC TTGCTTTACT CGCTGGAGAA GGCTATTTGG 
AACTGGATCG AGTACCATCC ACAAGAGTTC CAGGACTTGC AACGAGGAAC CAATCGAGAT 
ATATCAACCT GCTGGGAACC CCTGATGGAC TTTGTAGAGT ACTTTAAAAC CGAAAACAAG 
AAAAGCAAGA CTCTTGTTTG GCCGCTTCAA ATGCTCTTGC TGATATTGAA TCCCTCTTGC 
CTTGAGGCTG TTGTTAACGA ACTTCAGCAG TCGGAGAAAG AGAAGGAAAA GGACAAGGAA 
AAGGTTGCCT CGAAGTCAGC GCAATCCACG TCCCGGGACA AGGACTTCTC GGCCAAGCAG 
TTCATCGAGA GTATCAAGAG AGGCTTGGGC CAGCACTCAC CATCCAAACA GGTGACCGAA 
TCTGCGGCAA TTGCCTGTGT AAAGCTGTGC AAAGCATCCA CCTACATCAA CAACACCGAC 
TCCAATAATG TGGTCTTCAA GTTGGTGCAA TTCTTTATCA ACGATCTCAA GGCACTGCTC 
TTTAATCCAG CAAAGCCCTT TTCGCGCGGT CAGGGGTACA ACTTCGCTGA TATCGAGCTG 
ATGATTGACT GCTGGGTGTC TTGTTTCCGG ATTAATCCTC ACAACATCGA GGCTCTAAAG 
GTTTGCCTGA ATTTGTCCTC GCCGCAGGCT TACCATTTTG TAATTGTGTG CTCTCTGCTA 
AGGTTGGCTC ACATATACGT TGACTTCCGT TTGCAGAACA AGAACCCCTT TCGAATAGTA 
AACCAACCGA GATTGTCTTG GTGGCCGCAA ACGGATGTGG TTCACTACCG TTCTGCTGAG 
CTACGAGCTT TGTTTACGGA TACGCTCAAC AAGGCCACCC AGGGTTATAT AGCCCATACG 
CCACTGCGCT ACATAACCTC CCTGACGCTC AAATCTAAAG ATACCCAGAA AGGTTTAACC 
CGCGCTGAAG AGGGTCCCGC CCACAAAATG CTATTACTGC TACTCGTACG GCTAATTCAT 
GCAGATCCCA CGCTTTTGTT GAATACCCAA GGAAAAGTGG CACATGAAGT GCAGAGCTCC 
ACATTGGAGC TCATCAACGG CTTAGTTAGT TTGGTGCATC AAACTACCAT GCCCGATGTG 
GCACAAGAGG CTATGGAGGC GCTGCTTGCT CTGCACGCCC CGGAGAAAAT AGAGGTTTGG 
AATCCAGAGG CTCCGATCAA TACGTTCTGG GACGTTAGCT CCCAAGTACT GTTTTCAATC 
TCGCAAAAGC TCATTCAACA CCAAATTGCC AATTACACTG ATGTCTTAAA GTGGCTACGC 
GAGATCCTCA TCTGTCGGAA TACTTTTCTT CAGCGACACA AGGATTATGC ACATGTGGGA 
AGTCAGATTG CCATTTGCAA GCAGGCTCAC ATTAAGATGG AAGTTGTTTT CTTCATGTAC 
CTGTGGAGTG TTGATTTAGA TGCGGTGCTG ACATCGCTAT CGTGTTTTGG ACTACTATGC 
GAGGAGGCTG AGATTTGCTG CAGTTCTGAT GAATTGACAG TCGGATTTAT TATGCCGAAT 
TACCACATTT ACCAGGAGCT GGCTCAGTTG TCCACATCTG CAACGGACTC TCGCATTTGC 
TTTTTTGACA ACACGCACGG CAATGTGCTG AGCCGTCTTA CACTTCAAAA GCGCATAATG 
ACCCTATTGC GCAAGATCGA GCACTGTGTT CATGGTGTTC AGCCCGCCTG GGAGGAAACC 
TTCCGCAACT GGGAAGTATC CAGCAAGGTT TTGCAAACGT ACCCCAAATG TAAAGGAGAA 
GATGGCCAAG CGGAGGTTTT CCATCGTGGC ATGGGCAAGC GGCGAGCGAG TCACCAAAGC 
TCAGAGCACG ATTTGGAGGA ACAAATTAAT GAATGGGCCA ATATGACATG GTTTCTATTA 
GCCTTGGGTG GCGTCTGCCT TCATAAACGT AGCAGCAGTC GTCAAATGCT GCTGCAGCAA 
TCACAGAACA ATGCTTCTTT GGGATCACTT GCTCAAAACT CTCTTTACTC AAGCTCCACA 
AGTTCTGGAC ATGGCTCTCT GCATCCTAGC ACGGTTTCAC TATCCACTCT TCCCCCAGCA 
CCGCCACAAG ATGTCAGCTA TTGTCCTGTA ACACAATTCG TGGGCCAACT ATTGCGCCTG 
TTAGTTTGCA GCAACGAAAA AATTGGACTT AATATTCAGA AAAATGTGAA GGAACTGGTG 
GGCGAGGAGA TGTCCACCCA ACTGTATCCT ATACTCTTCG ACCAGGTTAG AGCCATCGTG 
GAGAAGTTCT TCGATCAGCA AGGTCAGGTG AACGTCAATG TGACCGACAT CAACACGCAG 
TTTATCGAGC ACACCATCTA CATAATGAAA TCAATTCTGG ATCCCAAAGC CAACAAGGAT 
CCCAACAACG ATCAACCCTC GCCATCGGAA CATTTAGGTG TTACAAGCAT CGAAGGCATG 
ATGCTAGGAA TAGTGCGCTA CGTTCGCCAC CTGGATATGA CTGTTTATGC CATTCGAATC 
AAGACAAAAT TGTGCCAGCT TGTGGAAGTA ATGATGAAGC GACGCGACGA CCTCGCCTTT 
CGCCAGGAGA TGAAGTTCCG GAATAAACTG GTTGAATACT TGACCGACTG GGTGATGGGC 
ACCTCCCATC AGATTGCACC GCCCAGCTCG GCGGATGCCG CTATTCTTAC CAACACATCC 
CTCATATTTC GCGATCTAGA CCAAGCCTGC ATGGAGGCAG TGGCTGCCTT GCTTCGGGGC 
CTTCCCCTTC AGCCTGAGGA ATCGGATCGA GGCGATTTGA TGGATGCAAA GAGTGCGCTC 
TTTTTAAAGT ACTTTACCCT ATTTATGAAC CTGCTGAATG ATTGCATCGA TAGCTCCGAG 
GCGGAGAAGG AAATGAATAA TACCCCACTA TTGCCTCCGC GCCCTCGAAT GGCAGCTGGA 
AAACTGACCG CTCTGCGAAA TGCCACTATT CTAGCCATGT CTAATTTGTT GGGTGCCAAC 
ATTGACTCTG GTTTGATGCA CTCCATCGAT CTGGGTTATA ATCCTGATTT GCAAACGCGT 
GCCGCTTTCA TGGAGGTGTT GACTCAAATC CTGCAACAAG GCACCGAATT CGATACCTTG 
GCCGAAACTG TTTTAGCTGA TCGATTTGAG CAACTGGTCC AACTTGTTAC AATGATCAGC 
GACAAAGGAG AACTTCCCAT AGCAATGGCT TTGGCTAATG TGGTGACTAC ATCCCAAATG 
GACGAGCTGG CTAGGGTTCT GGTCACCCTA TTCGACGCCA AACACCTGTT ATCGCCACTC 
CTATGGAATA TGTTTTATCG CGAGGTGGAG GTCTCAGACT GCATGCAGAC ACTTTTCCGT 
GGAAATTCTT TGGGCAGCAA AATCATGGCC TTTTGCTTTA AAATATACGG CGCGAGCTAT 
CTCCAAATGC TACTAGAGCC TTTAATTCGT CCGCTTCTGG ATGAGGAGGA GGAGACCTGT 
TTTGAGGTGG ATCCAGCTCG TCTGGATCCA ACAGAAGATA TTGAACAGCA CAGGAACAAC 
TTGATTGCCC TAACGCAGAA GGTGTTTGAC GCTATTATCA ACTCGTCGGA TCGCTTTCCC 
CCGCAGCTGC GATCCATGTG CCATTGCCTG TACCAAGTGC TAAGCAAACG CTTCCCGAAT 
CTTCTTCAAA ACAATATCGG TGCTGTAGGC ACAGTCATCT TCTTGCGGTT CATCAATCCC 
GCCATAGTTT CCCCACAGGA ATTGGGAATC GTTGACAAAC AAGTGCACAG CTCGGCCAAA 
CGAGGTCTAA TGCTAATGTC CAAGATCCTT CAAAACATTG CTAACCACGT GGAGTTCTCC 
AAGGAGCAGC ACATGTTGTG TTTCAACGAT TTTCTGCGCG ATCACTTTGA AGCTGGGCGG 
CGATTCTTCA TACAGATTGC ATCAGACTGT GAGACCGTGG ATCAGACCTC GCACAGCATG 
AGTTTTATTT CAGATGCGAA CGTACTGGCA CTGCATCGGT TGCTGTGGAC GCATCAGGAG 
AAGATCGGCG ACTATCTGTC TAGCAGTCGA GACCACAAGG CGGTTGGAAG GCGGCCCTTC 
GACAAGATGG CTACTTTGCT AGCATACCTG GGACCACCGG AGCACAAGCC CGTGGATTCG 
CACATGATGT TCAGCTCGTA TGCACGCTGG AGCTCCATTG ACATGTCGTC GACAAACTTC 
GAGGAGATCA TGGTCAAGCA CCAAATGCAC GAGAAGGAGG AATTCAAGAC CCTCAAGTCG 
ATGAACATAT TCTACCAGGC CGGAACAAGC AAATCTGGCT ATCCTGTCTT TTACTATATA 
GCAAGACGAT ACAAGATTGG AGAAACGAAT GGAGACTTAC TTATATATCA CGTCATACTC 
ACTCTGAAAC CATTCTGCCA CTCGCCCTTC GAGGTGGTCA TCGATTTTAC GCACACCTGT 
TCAGATAATC GGTTCCGCAC CGAGTTTCTG CAGAAGTGGT TTTATGTCCT GCCCACGGTA 
GCCTACGAAA ATGTCCATGC TGTGTACATC TATAACTGTA ACTCGTGGGT GCGCGAATAT 
ACCAAGTTCC ACGATCGCAT TCTGGCACCA TTAAAGGGAA ATCGCAAACT TCTGTTCCTG 
GAGTCACCCA ACAAACTTAC TGATTTCATC GACGCGGAGC AACAGAAATT GCCTGGAGCA 
ACTCTTTCCT TGGACGAAGA TTTAAAGGTA TTCAGCAACG CGCTGAAGCT CAGCCATAAA 
GACACAAAGG TGGCTATCAA AGTGGGTCCC ACCGCATTGC AAATTACATC TGCCGAAAAG 
ACAAAGGTCT TGGCTCACTC CGTGCTCCTG AACGATGTAT ACTACGCCTC TGAAATTGAG 
GAGGTGTGCT TGGTGGACGA TAACCAGTTC ACCCTGTCTA TAACTAACGA AAGTGGCCAG 
CTTAGCTTTA TTCACAACGA TTGCGACAAC ATTGTTCAAG CCATCATACA CATCCGAAAT 
CGATGGGAGC TTAGTCAGCC GGACTCCGTG ACGGTTCACC AAAAGATCCG ACCAAAAGAT 
GTGCCTGGAA CGCTGCTGAA CATGGCGCTA CTCAATCTGG GATCATGTGA CCCCAATCTA 
AGGACTGCTG CCTACAATTT GCTGTGTGCT TTGACCGCTA CTTTTGATCT AAAGATTGAG 
GGTCAACTGT TGGAGACCCA AGGACTTTGC ATACCCTCAA ATAATACCAT ATTTATCAAA 
TCCGTTAGCG AAAAGCTGGC CACCAATGAA CCGCATCTGA CTCTGGAATT CCTTGAGGAG 
TCCATTCAGG GCTTCCAGCG CAGCACCATA GAGCTGAAAC ATTTGTGTTT GGAGTACATG 
ACGCCATGGT TAAAGAACCT AGTCAAATTT TGCAAGTCCA ACGATGACTC CAAAAAGCTT 
AAGGTCTCCC AAATTCTGGA CAAGCTCATC AATTTAACTA TTGACCAAAA GGAAATGTAT 
CCCTCAGTGC AGGCCAAGAT CTGGGGATCA ATTGGACAGA TCCCCGAGCT GATCGACATG 
GTTTTGGATA ACTTTTTGCA CAAATCGATC ACATATGGCT TGGGATCACC ACAGGTGGAG 
ATTATGGCTG ACACGGCAGT GGCTCTCGCT TCAGCGAATG TTCAGCTGGT GTCCAAAAAG 
GTCATAACGC GAATATGCCG AGTCATGGAC AAATCCTGCA CAAATCCCAC ACAATATCTG 
GAGCAGCATA TGATGTGGGA CGATATTGCA ATTCTTGGTC GATACCTGCT CATGTTGTCG 
TTCAACAATT GTTTGGATGT GGCCACATCG GTGCCATATC TATTCCACAC CATTACGTTT 
TTGGTATGTT CAGGATCCCT GTCGATGCGA GCCTCTACTC ATGGCCTAGT GATCAACATC 
ATCCACTCGC TGTGCACATG CACAAATCCC TCATTTTCAG AGGAGGCGCA GCGAGTACTC 
CGACTGTCCC TGGATGAATT CTCACTGCCC AAGTTCTATC TACTCTTCGG CATCAGCAAG 
GTCAAGTCCG CAGCAGTTAC CGCTTTCCGC TCCAGCTGCC GTCACCCTAC AGATAAATGG 
CTAGGAAATG AAAGGGTTAC CCAGCCTCTG CCCGCCGATC GAGAGCGTTT ATCCCTACCA 
TCGCTTGAGG TTATCACGGA TGCCCTGCTG GAAATCATGG AGGCTTGCAT GCGAGATGTT 
CCGGACTGCG AATGGCTCAA CACCTGGACC TCTTTGGCTC GCAGTTTTGC TTTCTGTTAC 
AATCCAGCGC TACAGCCTAG AGCCCTTATA GTTTACGGGT GCATCAGCAA GAGTGTTACC 
GATCACGAGG TCAAGCAGTT ACTACGCATC CTGGTTAAGG CCCTCGAATC TTTCAACGAT 
CTCATTCTGA TCGAGGCTCT AGTAATGTGC TTAACTCGCA TTCAACCACT ACTCCGACCA 
GAGTCGCCCA TTCATCGGGC CCTCTTCTGG GTGGCCATTT CGGTGCTGCA GTTGGACGAG 
ATTACCCTGT ACGGCGCTGG ACTAGCTCTG CTTGAACAAA ATCTACACAC GCTTAAGTCA 
CAGGGCTGTT TTGACAAGAA GGAGACCATA GCCGAGGTCA TGATGAAGAC AAGGGAAAAG 
CTGGAGTGGC ATTTCAAGCA ACTGGATCAC GCCGTCGGCC TCTCCTTCCG CAGCAATTTC 
CACTTTGCCC TAGTGGGACA CTTGATTAAG GGCTTCCGCC ATCCTACACC TACAACCGTC 
TCACGCACCT CTCGTGTGCT GACGATGCTC TTGGGCATAA TAGCCAAACC CCTGCATCGG 
GACAAGTTCG AAGTAACACC TGACAGTGTG GCCTATTTGA CTGCGCTGGT GGCTGTCTCC 
GAGGAAGTCC GTTCCAGATG TCACGTAAAA CATGCCCTTC CCCGCTGGCC AGCCGATCTG 
AGTAGTAGTG TGGAAAATGG TGAAGCATCC GGCGGAGTGC AAGCTATCGG CCTGCCCCTG 
TCACGCCGTC AAAAAAGTTG GGACATCCTG GATCAGTCCG CCTTGCAGTT TGCCCGCCAA 
CACAAGGTTC CCACTCTTCA GAACGCGCGA GTTTTGTTCA AAACTCAACG CTCATTCTCG 
GTGCCGACGA CCAAAGATCC GAACAATGCC ACCGGCATCG AAGAACGTCA GGAACGCGGT 
TCGCGTTCGT CGGTGTCCAA CGAGTCCAAC GTACTGCTCG ATCCCGAGGT CTTACCTGAC 
CTGTCCATTC AAGCCCTGGT ACTGACTGTT TTGGCCACCT TGGTCAAGTA CTCTTCGGAT 
GAAGGCGAAA CGCGGGTTTT GTATCAGTAT TTGGCCGAAG GATCGGTGGT CTTTCCAAAG 
GTGTTTCCAG TTATCCATTC ACTGCTGGAC CAAAAGATAA ACAACATTTT GTCAGTGTCA 
CACGACCAAG TGGTGCTCAA CTCTGTGCAG AACATTATAC AAAATATGCT GGCCAGCGAA 
GATCCTTCCC AGCAGCAGCT GCACTTCCTG CAGAGTTGTG GATTTGGCGG ACTCTGGCGA 
TTTGCTGGTC CCTTCACCAA GTACAATATG ATGGGTGAGT CCTCGGAGCT GTTTGTCAAC 
TGTTTGGAGG CCATGGTTGA GACCTGTTTG CCAGGCGACG AATCCGCGCC GGTGCCACCA 
TCTCCGCGTC CATACAACCT GAGCTCCAGT CTGAGTAGCC TGACCCTGGG ATCACCCACG 
GATAAAGCTG CATGAATAAC AACTACAAAC CCGCGCACTA GACGTACCAC ACTTATCGCA 
AAAGTAATGC GTTTCCTAAA TCTGCTTAAA AAGTGCTTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nf1 ( NM_001276050.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262979.1 Drosophila melanogaster neurofibromin 1, transcript variant F (Nf1), mRNA.

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CloneID	OFa08850
Clone ID Related Accession (Same CDS sequence)	NM_001276050.1
Accession Version	NM_001276050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	neurofibromin 1, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCCAGA AGCCAGGCGA GTGGGCTAGC GCCCTTTTGG CACGTTTCGA GGATCAGCTG 
CCAAACAGAA TCGGTGCCTA TGGCACGCAG GCCAGGATGA GCCAGGACCA ATTGGTGGCC 
TGTCTGATCC ACATATCGCG CTACCGCTTC TCCCTTGTCA TATCCGGTCT CACCAAGATG 
CTGCAGAGGG TCAATGAGGC GGCCCTTCAA AATCGCCATG AGCCAGAGCG TTGCTACTTT 
GAGTCGCTGG TCATCATACT GACCACTCTG GAACGCTGTC TGACCAACCA GACCAAGGAC 
ACAGCACGTT TTGAAGAGGC CATGAATGTG AAGCTTCTGC TGCGCGAGAT CTCCCAGTTT 
GTCGACGTCC AGAGCGACAG CAATCCGAAT GCCGCCCAGC TCAAGGCCCT GGCATCCAAG 
GTGCTCTTTG CCTTGTCCCA GAATCACTTC TCGGCGGTGT TCAATCGCAT ATCGGCCAGG 
ATTCAAGAGC TGACATCCTG TTCCGAGGAA AACCCGGACT ACAACGACAT AGAATTAATT 
CAACACATCG ATATGGATAT GATAAAGCTA ACCAAGCTGC TACAAGAAAC CATCACAAAG 
TTTCGATCGA AGCGTGCTCC ACCTTTGATC TTGCTTTACT CGCTGGAGAA GGCTATTTGG 
AACTGGATCG AGTACCATCC ACAAGAGTTC CAGGACTTGC AACGAGGAAC CAATCGAGAT 
ATATCAACCT GCTGGGAACC CCTGATGGAC TTTGTAGAGT ACTTTAAAAC CGAAAACAAG 
AAAAGCAAGA CTCTTGTTTG GCCGCTTCAA ATGCTCTTGC TGATATTGAA TCCCTCTTGC 
CTTGAGGCTG TTGTTAACGA ACTTCAGCAG TCGGAGAAAG AGAAGGAAAA GGACAAGGAA 
AAGGTTGCCT CGAAGTCAGC GCAATCCACG TCCCGGGACA AGGACTTCTC GGCCAAGCAG 
TTCATCGAGA GTATCAAGAG AGGCTTGGGC CAGCACTCAC CATCCAAACA GGTGACCGAA 
TCTGCGGCAA TTGCCTGTGT AAAGCTGTGC AAAGCATCCA CCTACATCAA CAACACCGAC 
TCCAATAATG TGGTCTTCAA GTTGGTGCAA TTCTTTATCA ACGATCTCAA GGCACTGCTC 
TTTAATCCAG CAAAGCCCTT TTCGCGCGGT CAGGGGTACA ACTTCGCTGA TATCGAGCTG 
ATGATTGACT GCTGGGTGTC TTGTTTCCGG ATTAATCCTC ACAACATCGA GGCTCTAAAG 
GTTTGCCTGA ATTTGTCCTC GCCGCAGGCT TACCATTTTG TAATTGTGTG CTCTCTGCTA 
AGGTTGGCTC ACATATACGT TGACTTCCGT TTGCAGAACA AGAACCCCTT TCGAATAGTA 
AACCAACCGA GATTGTCTTG GTGGCCGCAA ACGGATGTGG TTCACTACCG TTCTGCTGAG 
CTACGAGCTT TGTTTACGGA TACGCTCAAC AAGGCCACCC AGGGTTATAT AGCCCATACG 
CCACTGCGCT ACATAACCTC CCTGACGCTC AAATCTAAAG ATACCCAGAA AGGTTTAACC 
CGCGCTGAAG AGGGTCCCGC CCACAAAATG CTATTACTGC TACTCGTACG GCTAATTCAT 
GCAGATCCCA CGCTTTTGTT GAATACCCAA GGAAAAGTGG CACATGAAGT GCAGAGCTCC 
ACATTGGAGC TCATCAACGG CTTAGTTAGT TTGGTGCATC AAACTACCAT GCCCGATGTG 
GCACAAGAGG CTATGGAGGC GCTGCTTGCT CTGCACGCCC CGGAGAAAAT AGAGGTTTGG 
AATCCAGAGG CTCCGATCAA TACGTTCTGG GACGTTAGCT CCCAAGTACT GTTTTCAATC 
TCGCAAAAGC TCATTCAACA CCAAATTGCC AATTACACTG ATGTCTTAAA GTGGCTACGC 
GAGATCCTCA TCTGTCGGAA TACTTTTCTT CAGCGACACA AGGATTATGC ACATGTGGGA 
AGTCAGATTG CCATTTGCAA GCAGGCTCAC ATTAAGATGG AAGTTGTTTT CTTCATGTAC 
CTGTGGAGTG TTGATTTAGA TGCGGTGCTG ACATCGCTAT CGTGTTTTGG ACTACTATGC 
GAGGAGGCTG AGATTTGCTG CAGTTCTGAT GAATTGACAG TCGGATTTAT TATGCCGAAT 
TACCACATTT ACCAGGAGCT GGCTCAGTTG TCCACATCTG CAACGGACTC TCGCATTTGC 
TTTTTTGACA ACACGCACGG CAATGTGCTG AGCCGTCTTA CACTTCAAAA GCGCATAATG 
ACCCTATTGC GCAAGATCGA GCACTGTGTT CATGGTGTTC AGCCCGCCTG GGAGGAAACC 
TTCCGCAACT GGGAAGTATC CAGCAAGGTT TTGCAAACGT ACCCCAAATG TAAAGGAGAA 
GATGGCCAAG CGGAGGTTTT CCATCGTGGC ATGGGCAAGC GGCGAGCGAG TCACCAAAGC 
TCAGAGCACG ATTTGGAGGA ACAAATTAAT GAATGGGCCA ATATGACATG GTTTCTATTA 
GCCTTGGGTG GCGTCTGCCT TCATAAACGT AGCAGCAGTC GTCAAATGCT GCTGCAGCAA 
TCACAGAACA ATGCTTCTTT GGGATCACTT GCTCAAAACT CTCTTTACTC AAGCTCCACA 
AGTTCTGGAC ATGGCTCTCT GCATCCTAGC ACGGTTTCAC TATCCACTCT TCCCCCAGCA 
CCGCCACAAG ATGTCAGCTA TTGTCCTGTA ACACAATTCG TGGGCCAACT ATTGCGCCTG 
TTAGTTTGCA GCAACGAAAA AATTGGACTT AATATTCAGA AAAATGTGAA GGAACTGGTG 
GGCGAGGAGA TGTCCACCCA ACTGTATCCT ATACTCTTCG ACCAGGTTAG AGCCATCGTG 
GAGAAGTTCT TCGATCAGCA AGGTCAGGTG AACGTCAATG TGACCGACAT CAACACGCAG 
TTTATCGAGC ACACCATCTA CATAATGAAA TCAATTCTGG ATCCCAAAGC CAACAAGGAT 
CCCAACAACG ATCAACCCTC GCCATCGGAA CATTTAGGTG TTACAAGCAT CGAAGGCATG 
ATGCTAGGAA TAGTGCGCTA CGTTCGCCAC CTGGATATGA CTGTTTATGC CATTCGAATC 
AAGACAAAAT TGTGCCAGCT TGTGGAAGTA ATGATGAAGC GACGCGACGA CCTCGCCTTT 
CGCCAGGAGA TGAAGTTCCG GAATAAACTG GTTGAATACT TGACCGACTG GGTGATGGGC 
ACCTCCCATC AGATTGCACC GCCCAGCTCG GCGGATGCCG CTATTCTTAC CAACACATCC 
CTCATATTTC GCGATCTAGA CCAAGCCTGC ATGGAGGCAG TGGCTGCCTT GCTTCGGGGC 
CTTCCCCTTC AGCCTGAGGA ATCGGATCGA GGCGATTTGA TGGATGCAAA GAGTGCGCTC 
TTTTTAAAGT ACTTTACCCT ATTTATGAAC CTGCTGAATG ATTGCATCGA TAGCTCCGAG 
GCGGAGAAGG AAATGAATAA TACCCCACTA TTGCCTCCGC GCCCTCGAAT GGCAGCTGGA 
AAACTGACCG CTCTGCGAAA TGCCACTATT CTAGCCATGT CTAATTTGTT GGGTGCCAAC 
ATTGACTCTG GTTTGATGCA CTCCATCGAT CTGGGTTATA ATCCTGATTT GCAAACGCGT 
GCCGCTTTCA TGGAGGTGTT GACTCAAATC CTGCAACAAG GCACCGAATT CGATACCTTG 
GCCGAAACTG TTTTAGCTGA TCGATTTGAG CAACTGGTCC AACTTGTTAC AATGATCAGC 
GACAAAGGAG AACTTCCCAT AGCAATGGCT TTGGCTAATG TGGTGACTAC ATCCCAAATG 
GACGAGCTGG CTAGGGTTCT GGTCACCCTA TTCGACGCCA AACACCTGTT ATCGCCACTC 
CTATGGAATA TGTTTTATCG CGAGGTGGAG GTCTCAGACT GCATGCAGAC ACTTTTCCGT 
GGAAATTCTT TGGGCAGCAA AATCATGGCC TTTTGCTTTA AAATATACGG CGCGAGCTAT 
CTCCAAATGC TACTAGAGCC TTTAATTCGT CCGCTTCTGG ATGAGGAGGA GGAGACCTGT 
TTTGAGGTGG ATCCAGCTCG TCTGGATCCA ACAGAAGATA TTGAACAGCA CAGGAACAAC 
TTGATTGCCC TAACGCAGAA GGTGTTTGAC GCTATTATCA ACTCGTCGGA TCGCTTTCCC 
CCGCAGCTGC GATCCATGTG CCATTGCCTG TACCAAGTGC TAAGCAAACG CTTCCCGAAT 
CTTCTTCAAA ACAATATCGG TGCTGTAGGC ACAGTCATCT TCTTGCGGTT CATCAATCCC 
GCCATAGTTT CCCCACAGGA ATTGGGAATC GTTGACAAAC AAGTGCACAG CTCGGCCAAA 
CGAGGTCTAA TGCTAATGTC CAAGATCCTT CAAAACATTG CTAACCACGT GGAGTTCTCC 
AAGGAGCAGC ACATGTTGTG TTTCAACGAT TTTCTGCGCG ATCACTTTGA AGCTGGGCGG 
CGATTCTTCA TACAGATTGC ATCAGACTGT GAGACCGTGG ATCAGACCTC GCACAGCATG 
AGTTTTATTT CAGATGCGAA CGTACTGGCA CTGCATCGGT TGCTGTGGAC GCATCAGGAG 
AAGATCGGCG ACTATCTGTC TAGCAGTCGA GACCACAAGG CGGTTGGAAG GCGGCCCTTC 
GACAAGATGG CTACTTTGCT AGCATACCTG GGACCACCGG AGCACAAGCC CGTGGATTCG 
CACATGATGT TCAGCTCGTA TGCACGCTGG AGCTCCATTG ACATGTCGTC GACAAACTTC 
GAGGAGATCA TGGTCAAGCA CCAAATGCAC GAGAAGGAGG AATTCAAGAC CCTCAAGTCG 
ATGAACATAT TCTACCAGGC CGGAACAAGC AAATCTGGCT ATCCTGTCTT TTACTATATA 
GCAAGACGAT ACAAGATTGG AGAAACGAAT GGAGACTTAC TTATATATCA CGTCATACTC 
ACTCTGAAAC CATTCTGCCA CTCGCCCTTC GAGGTGGTCA TCGATTTTAC GCACACCTGT 
TCAGATAATC GGTTCCGCAC CGAGTTTCTG CAGAAGTGGT TTTATGTCCT GCCCACGGTA 
GCCTACGAAA ATGTCCATGC TGTGTACATC TATAACTGTA ACTCGTGGGT GCGCGAATAT 
ACCAAGTTCC ACGATCGCAT TCTGGCACCA TTAAAGGGAA ATCGCAAACT TCTGTTCCTG 
GAGTCACCCA ACAAACTTAC TGATTTCATC GACGCGGAGC AACAGAAATT GCCTGGAGCA 
ACTCTTTCCT TGGACGAAGA TTTAAAGGTA TTCAGCAACG CGCTGAAGCT CAGCCATAAA 
GACACAAAGG TGGCTATCAA AGTGGGTCCC ACCGCATTGC AAATTACATC TGCCGAAAAG 
ACAAAGGTCT TGGCTCACTC CGTGCTCCTG AACGATGTAT ACTACGCCTC TGAAATTGAG 
GAGGTGTGCT TGGTGGACGA TAACCAGTTC ACCCTGTCTA TAACTAACGA AAGTGGCCAG 
CTTAGCTTTA TTCACAACGA TTGCGACAAC ATTGTTCAAG CCATCATACA CATCCGAAAT 
CGATGGGAGC TTAGTCAGCC GGACTCCGTG ACGGTTCACC AAAAGATCCG ACCAAAAGAT 
GTGCCTGGAA CGCTGCTGAA CATGGCGCTA CTCAATCTGG GATCATGTGA CCCCAATCTA 
AGGACTGCTG CCTACAATTT GCTGTGTGCT TTGACCGCTA CTTTTGATCT AAAGATTGAG 
GGTCAACTGT TGGAGACCCA AGGACTTTGC ATACCCTCAA ATAATACCAT ATTTATCAAA 
TCCGTTAGCG AAAAGCTGGC CACCAATGAA CCGCATCTGA CTCTGGAATT CCTTGAGGAG 
TCCATTCAGG GCTTCCAGCG CAGCACCATA GAGCTGAAAC ATTTGTGTTT GGAGTACATG 
ACGCCATGGT TAAAGAACCT AGTCAAATTT TGCAAGTCCA ACGATGACTC CAAAAAGCTT 
AAGGTCTCCC AAATTCTGGA CAAGCTCATC AATTTAACTA TTGACCAAAA GGAAATGTAT 
CCCTCAGTGC AGGCCAAGAT CTGGGGATCA ATTGGACAGA TCCCCGAGCT GATCGACATG 
GTTTTGGATA ACTTTTTGCA CAAATCGATC ACATATGGCT TGGGATCACC ACAGGTGGAG 
ATTATGGCTG ACACGGCAGT GGCTCTCGCT TCAGCGAATG TTCAGCTGGT GTCCAAAAAG 
GTCATAACGC GAATATGCCG AGTCATGGAC AAATCCTGCA CAAATCCCAC ACAATATCTG 
GAGCAGCATA TGATGTGGGA CGATATTGCA ATTCTTGGTC GATACCTGCT CATGTTGTCG 
TTCAACAATT GTTTGGATGT GGCCACATCG GTGCCATATC TATTCCACAC CATTACGTTT 
TTGGTATGTT CAGGATCCCT GTCGATGCGA GCCTCTACTC ATGGCCTAGT GATCAACATC 
ATCCACTCGC TGTGCACATG CACAAATCCC TCATTTTCAG AGGAGGCGCA GCGAGTACTC 
CGACTGTCCC TGGATGAATT CTCACTGCCC AAGTTCTATC TACTCTTCGG CATCAGCAAG 
GTCAAGTCCG CAGCAGTTAC CGCTTTCCGC TCCAGCTGCC GTCACCCTAC AGATAAATGG 
CTAGGAAATG AAAGGGTTAC CCAGCCTCTG CCCGCCGATC GAGAGCGTTT ATCCCTACCA 
TCGCTTGAGG TTATCACGGA TGCCCTGCTG GAAATCATGG AGGCTTGCAT GCGAGATGTT 
CCGGACTGCG AATGGCTCAA CACCTGGACC TCTTTGGCTC GCAGTTTTGC TTTCTGTTAC 
AATCCAGCGC TACAGCCTAG AGCCCTTATA GTTTACGGGT GCATCAGCAA GAGTGTTACC 
GATCACGAGG TCAAGCAGTT ACTACGCATC CTGGTTAAGG CCCTCGAATC TTTCAACGAT 
CTCATTCTGA TCGAGGCTCT AGTAATGTGC TTAACTCGCA TTCAACCACT ACTCCGACCA 
GAGTCGCCCA TTCATCGGGC CCTCTTCTGG GTGGCCATTT CGGTGCTGCA GTTGGACGAG 
ATTACCCTGT ACGGCGCTGG ACTAGCTCTG CTTGAACAAA ATCTACACAC GCTTAAGTCA 
CAGGGCTGTT TTGACAAGAA GGAGACCATA GCCGAGGTCA TGATGAAGAC AAGGGAAAAG 
CTGGAGTGGC ATTTCAAGCA ACTGGATCAC GCCGTCGGCC TCTCCTTCCG CAGCAATTTC 
CACTTTGCCC TAGTGGGACA CTTGATTAAG GGCTTCCGCC ATCCTACACC TACAACCGTC 
TCACGCACCT CTCGTGTGCT GACGATGCTC TTGGGCATAA TAGCCAAACC CCTGCATCGG 
GACAAGTTCG AAGTAACACC TGACAGTGTG GCCTATTTGA CTGCGCTGGT GGCTGTCTCC 
GAGGAAGTCC GTTCCAGATG TCACGTAAAA CATGCCCTTC CCCGCTGGCC AGCCGATCTG 
AGTAGTAGTG TGGAAAATGG TGAAGCATCC GGCGGAGTGC AAGCTATCGG CCTGCCCCTG 
TCACGCCGTC AAAAAAGTTG GGACATCCTG GATCAGTCCG CCTTGCAGTT TGCCCGCCAA 
CACAAGGTTC CCACTCTTCA GGAACGCGGT TCGCGTTCGT CGGTGTCCAA CGAGTCCAAC 
GTACTGCTCG ATCCCGAGGT CTTACCTGAC CTGTCCATTC AAGCCCTGGT ACTGACTGTT 
TTGGCCACCT TGGTCAAGTA CTCTTCGGAT GAAGGCGAAA CGCGGGTTTT GTATCAGTAT 
TTGGCCGAAG GATCGGTGGT CTTTCCAAAG GTGTTTCCAG TTATCCATTC ACTGCTGGAC 
CAAAAGATAA ACAACATTTT GTCAGTGTCA CACGACCAAG TGGTGCTCAA CTCTGTGCAG 
AACATTATAC AAAATATGCT GGCCAGCGAA GATCCTTCCC AGCAGCAGCT GCACTTCCTG 
CAGAGTTGTG GATTTGGCGG ACTCTGGCGA TTTGCTGGTC CCTTCACCAA GTACAATATG 
ATGGGTGAGT CCTCGGAGCT GTTTGTCAAC TGTTTGGAGG CCATGGTTGA GACCTGTTTG 
CCAGGCGACG AATCCGCGCC GGTGCCACCA TCTCCGCGTC CATACAACCT GAGCTCCAGT 
CTGAGTAGCC TGACCCTGGG ATCACCCACG GATAAAGCTG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262979.1
CDS	280..8484
Translation	MTQKPGEWASALLARFEDQLPNRIGAYGTQARMSQDQLVACLIHISRYRFSLVISGLTKMLQRVNEAALQNRHEPERCYFESLVIILTTLERCLTNQTKDTARFEEAMNVKLLLREISQFVDVQSDSNPNAAQLKALASKVLFALSQNHFSAVFNRISARIQELTSCSEENPDYNDIELIQHIDMDMIKLTKLLQETITKFRSKRAPPLILLYSLEKAIWNWIEYHPQEFQDLQRGTNRDISTCWEPLMDFVEYFKTENKKSKTLVWPLQMLLLILNPSCLEAVVNELQQSEKEKEKDKEKVASKSAQSTSRDKDFSAKQFIESIKRGLGQHSPSKQVTESAAIACVKLCKASTYINNTDSNNVVFKLVQFFINDLKALLFNPAKPFSRGQGYNFADIELMIDCWVSCFRINPHNIEALKVCLNLSSPQAYHFVIVCSLLRLAHIYVDFRLQNKNPFRIVNQPRLSWWPQTDVVHYRSAELRALFTDTLNKATQGYIAHTPLRYITSLTLKSKDTQKGLTRAEEGPAHKMLLLLLVRLIHADPTLLLNTQGKVAHEVQSSTLELINGLVSLVHQTTMPDVAQEAMEALLALHAPEKIEVWNPEAPINTFWDVSSQVLFSISQKLIQHQIANYTDVLKWLREILICRNTFLQRHKDYAHVGSQIAICKQAHIKMEVVFFMYLWSVDLDAVLTSLSCFGLLCEEAEICCSSDELTVGFIMPNYHIYQELAQLSTSATDSRICFFDNTHGNVLSRLTLQKRIMTLLRKIEHCVHGVQPAWEETFRNWEVSSKVLQTYPKCKGEDGQAEVFHRGMGKRRASHQSSEHDLEEQINEWANMTWFLLALGGVCLHKRSSSRQMLLQQSQNNASLGSLAQNSLYSSSTSSGHGSLHPSTVSLSTLPPAPPQDVSYCPVTQFVGQLLRLLVCSNEKIGLNIQKNVKELVGEEMSTQLYPILFDQVRAIVEKFFDQQGQVNVNVTDINTQFIEHTIYIMKSILDPKANKDPNNDQPSPSEHLGVTSIEGMMLGIVRYVRHLDMTVYAIRIKTKLCQLVEVMMKRRDDLAFRQEMKFRNKLVEYLTDWVMGTSHQIAPPSSADAAILTNTSLIFRDLDQACMEAVAALLRGLPLQPEESDRGDLMDAKSALFLKYFTLFMNLLNDCIDSSEAEKEMNNTPLLPPRPRMAAGKLTALRNATILAMSNLLGANIDSGLMHSIDLGYNPDLQTRAAFMEVLTQILQQGTEFDTLAETVLADRFEQLVQLVTMISDKGELPIAMALANVVTTSQMDELARVLVTLFDAKHLLSPLLWNMFYREVEVSDCMQTLFRGNSLGSKIMAFCFKIYGASYLQMLLEPLIRPLLDEEEETCFEVDPARLDPTEDIEQHRNNLIALTQKVFDAIINSSDRFPPQLRSMCHCLYQVLSKRFPNLLQNNIGAVGTVIFLRFINPAIVSPQELGIVDKQVHSSAKRGLMLMSKILQNIANHVEFSKEQHMLCFNDFLRDHFEAGRRFFIQIASDCETVDQTSHSMSFISDANVLALHRLLWTHQEKIGDYLSSSRDHKAVGRRPFDKMATLLAYLGPPEHKPVDSHMMFSSYARWSSIDMSSTNFEEIMVKHQMHEKEEFKTLKSMNIFYQAGTSKSGYPVFYYIARRYKIGETNGDLLIYHVILTLKPFCHSPFEVVIDFTHTCSDNRFRTEFLQKWFYVLPTVAYENVHAVYIYNCNSWVREYTKFHDRILAPLKGNRKLLFLESPNKLTDFIDAEQQKLPGATLSLDEDLKVFSNALKLSHKDTKVAIKVGPTALQITSAEKTKVLAHSVLLNDVYYASEIEEVCLVDDNQFTLSITNESGQLSFIHNDCDNIVQAIIHIRNRWELSQPDSVTVHQKIRPKDVPGTLLNMALLNLGSCDPNLRTAAYNLLCALTATFDLKIEGQLLETQGLCIPSNNTIFIKSVSEKLATNEPHLTLEFLEESIQGFQRSTIELKHLCLEYMTPWLKNLVKFCKSNDDSKKLKVSQILDKLINLTIDQKEMYPSVQAKIWGSIGQIPELIDMVLDNFLHKSITYGLGSPQVEIMADTAVALASANVQLVSKKVITRICRVMDKSCTNPTQYLEQHMMWDDIAILGRYLLMLSFNNCLDVATSVPYLFHTITFLVCSGSLSMRASTHGLVINIIHSLCTCTNPSFSEEAQRVLRLSLDEFSLPKFYLLFGISKVKSAAVTAFRSSCRHPTDKWLGNERVTQPLPADRERLSLPSLEVITDALLEIMEACMRDVPDCEWLNTWTSLARSFAFCYNPALQPRALIVYGCISKSVTDHEVKQLLRILVKALESFNDLILIEALVMCLTRIQPLLRPESPIHRALFWVAISVLQLDEITLYGAGLALLEQNLHTLKSQGCFDKKETIAEVMMKTREKLEWHFKQLDHAVGLSFRSNFHFALVGHLIKGFRHPTPTTVSRTSRVLTMLLGIIAKPLHRDKFEVTPDSVAYLTALVAVSEEVRSRCHVKHALPRWPADLSSSVENGEASGGVQAIGLPLSRRQKSWDILDQSALQFARQHKVPTLQERGSRSSVSNESNVLLDPEVLPDLSIQALVLTVLATLVKYSSDEGETRVLYQYLAEGSVVFPKVFPVIHSLLDQKINNILSVSHDQVVLNSVQNIIQNMLASEDPSQQQLHFLQSCGFGGLWRFAGPFTKYNMMGESSELFVNCLEAMVETCLPGDESAPVPPSPRPYNLSSSLSSLTLGSPTDKAA

Target ORF information:

RefSeq Version	NM_001276050.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster neurofibromin 1, transcript variant F (Nf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276050.1

ORF Insert Sequence:

ATGACCCAGA AGCCAGGCGA GTGGGCTAGC GCCCTTTTGG CACGTTTCGA GGATCAGCTG 
CCAAACAGAA TCGGTGCCTA TGGCACGCAG GCCAGGATGA GCCAGGACCA ATTGGTGGCC 
TGTCTGATCC ACATATCGCG CTACCGCTTC TCCCTTGTCA TATCCGGTCT CACCAAGATG 
CTGCAGAGGG TCAATGAGGC GGCCCTTCAA AATCGCCATG AGCCAGAGCG TTGCTACTTT 
GAGTCGCTGG TCATCATACT GACCACTCTG GAACGCTGTC TGACCAACCA GACCAAGGAC 
ACAGCACGTT TTGAAGAGGC CATGAATGTG AAGCTTCTGC TGCGCGAGAT CTCCCAGTTT 
GTCGACGTCC AGAGCGACAG CAATCCGAAT GCCGCCCAGC TCAAGGCCCT GGCATCCAAG 
GTGCTCTTTG CCTTGTCCCA GAATCACTTC TCGGCGGTGT TCAATCGCAT ATCGGCCAGG 
ATTCAAGAGC TGACATCCTG TTCCGAGGAA AACCCGGACT ACAACGACAT AGAATTAATT 
CAACACATCG ATATGGATAT GATAAAGCTA ACCAAGCTGC TACAAGAAAC CATCACAAAG 
TTTCGATCGA AGCGTGCTCC ACCTTTGATC TTGCTTTACT CGCTGGAGAA GGCTATTTGG 
AACTGGATCG AGTACCATCC ACAAGAGTTC CAGGACTTGC AACGAGGAAC CAATCGAGAT 
ATATCAACCT GCTGGGAACC CCTGATGGAC TTTGTAGAGT ACTTTAAAAC CGAAAACAAG 
AAAAGCAAGA CTCTTGTTTG GCCGCTTCAA ATGCTCTTGC TGATATTGAA TCCCTCTTGC 
CTTGAGGCTG TTGTTAACGA ACTTCAGCAG TCGGAGAAAG AGAAGGAAAA GGACAAGGAA 
AAGGTTGCCT CGAAGTCAGC GCAATCCACG TCCCGGGACA AGGACTTCTC GGCCAAGCAG 
TTCATCGAGA GTATCAAGAG AGGCTTGGGC CAGCACTCAC CATCCAAACA GGTGACCGAA 
TCTGCGGCAA TTGCCTGTGT AAAGCTGTGC AAAGCATCCA CCTACATCAA CAACACCGAC 
TCCAATAATG TGGTCTTCAA GTTGGTGCAA TTCTTTATCA ACGATCTCAA GGCACTGCTC 
TTTAATCCAG CAAAGCCCTT TTCGCGCGGT CAGGGGTACA ACTTCGCTGA TATCGAGCTG 
ATGATTGACT GCTGGGTGTC TTGTTTCCGG ATTAATCCTC ACAACATCGA GGCTCTAAAG 
GTTTGCCTGA ATTTGTCCTC GCCGCAGGCT TACCATTTTG TAATTGTGTG CTCTCTGCTA 
AGGTTGGCTC ACATATACGT TGACTTCCGT TTGCAGAACA AGAACCCCTT TCGAATAGTA 
AACCAACCGA GATTGTCTTG GTGGCCGCAA ACGGATGTGG TTCACTACCG TTCTGCTGAG 
CTACGAGCTT TGTTTACGGA TACGCTCAAC AAGGCCACCC AGGGTTATAT AGCCCATACG 
CCACTGCGCT ACATAACCTC CCTGACGCTC AAATCTAAAG ATACCCAGAA AGGTTTAACC 
CGCGCTGAAG AGGGTCCCGC CCACAAAATG CTATTACTGC TACTCGTACG GCTAATTCAT 
GCAGATCCCA CGCTTTTGTT GAATACCCAA GGAAAAGTGG CACATGAAGT GCAGAGCTCC 
ACATTGGAGC TCATCAACGG CTTAGTTAGT TTGGTGCATC AAACTACCAT GCCCGATGTG 
GCACAAGAGG CTATGGAGGC GCTGCTTGCT CTGCACGCCC CGGAGAAAAT AGAGGTTTGG 
AATCCAGAGG CTCCGATCAA TACGTTCTGG GACGTTAGCT CCCAAGTACT GTTTTCAATC 
TCGCAAAAGC TCATTCAACA CCAAATTGCC AATTACACTG ATGTCTTAAA GTGGCTACGC 
GAGATCCTCA TCTGTCGGAA TACTTTTCTT CAGCGACACA AGGATTATGC ACATGTGGGA 
AGTCAGATTG CCATTTGCAA GCAGGCTCAC ATTAAGATGG AAGTTGTTTT CTTCATGTAC 
CTGTGGAGTG TTGATTTAGA TGCGGTGCTG ACATCGCTAT CGTGTTTTGG ACTACTATGC 
GAGGAGGCTG AGATTTGCTG CAGTTCTGAT GAATTGACAG TCGGATTTAT TATGCCGAAT 
TACCACATTT ACCAGGAGCT GGCTCAGTTG TCCACATCTG CAACGGACTC TCGCATTTGC 
TTTTTTGACA ACACGCACGG CAATGTGCTG AGCCGTCTTA CACTTCAAAA GCGCATAATG 
ACCCTATTGC GCAAGATCGA GCACTGTGTT CATGGTGTTC AGCCCGCCTG GGAGGAAACC 
TTCCGCAACT GGGAAGTATC CAGCAAGGTT TTGCAAACGT ACCCCAAATG TAAAGGAGAA 
GATGGCCAAG CGGAGGTTTT CCATCGTGGC ATGGGCAAGC GGCGAGCGAG TCACCAAAGC 
TCAGAGCACG ATTTGGAGGA ACAAATTAAT GAATGGGCCA ATATGACATG GTTTCTATTA 
GCCTTGGGTG GCGTCTGCCT TCATAAACGT AGCAGCAGTC GTCAAATGCT GCTGCAGCAA 
TCACAGAACA ATGCTTCTTT GGGATCACTT GCTCAAAACT CTCTTTACTC AAGCTCCACA 
AGTTCTGGAC ATGGCTCTCT GCATCCTAGC ACGGTTTCAC TATCCACTCT TCCCCCAGCA 
CCGCCACAAG ATGTCAGCTA TTGTCCTGTA ACACAATTCG TGGGCCAACT ATTGCGCCTG 
TTAGTTTGCA GCAACGAAAA AATTGGACTT AATATTCAGA AAAATGTGAA GGAACTGGTG 
GGCGAGGAGA TGTCCACCCA ACTGTATCCT ATACTCTTCG ACCAGGTTAG AGCCATCGTG 
GAGAAGTTCT TCGATCAGCA AGGTCAGGTG AACGTCAATG TGACCGACAT CAACACGCAG 
TTTATCGAGC ACACCATCTA CATAATGAAA TCAATTCTGG ATCCCAAAGC CAACAAGGAT 
CCCAACAACG ATCAACCCTC GCCATCGGAA CATTTAGGTG TTACAAGCAT CGAAGGCATG 
ATGCTAGGAA TAGTGCGCTA CGTTCGCCAC CTGGATATGA CTGTTTATGC CATTCGAATC 
AAGACAAAAT TGTGCCAGCT TGTGGAAGTA ATGATGAAGC GACGCGACGA CCTCGCCTTT 
CGCCAGGAGA TGAAGTTCCG GAATAAACTG GTTGAATACT TGACCGACTG GGTGATGGGC 
ACCTCCCATC AGATTGCACC GCCCAGCTCG GCGGATGCCG CTATTCTTAC CAACACATCC 
CTCATATTTC GCGATCTAGA CCAAGCCTGC ATGGAGGCAG TGGCTGCCTT GCTTCGGGGC 
CTTCCCCTTC AGCCTGAGGA ATCGGATCGA GGCGATTTGA TGGATGCAAA GAGTGCGCTC 
TTTTTAAAGT ACTTTACCCT ATTTATGAAC CTGCTGAATG ATTGCATCGA TAGCTCCGAG 
GCGGAGAAGG AAATGAATAA TACCCCACTA TTGCCTCCGC GCCCTCGAAT GGCAGCTGGA 
AAACTGACCG CTCTGCGAAA TGCCACTATT CTAGCCATGT CTAATTTGTT GGGTGCCAAC 
ATTGACTCTG GTTTGATGCA CTCCATCGAT CTGGGTTATA ATCCTGATTT GCAAACGCGT 
GCCGCTTTCA TGGAGGTGTT GACTCAAATC CTGCAACAAG GCACCGAATT CGATACCTTG 
GCCGAAACTG TTTTAGCTGA TCGATTTGAG CAACTGGTCC AACTTGTTAC AATGATCAGC 
GACAAAGGAG AACTTCCCAT AGCAATGGCT TTGGCTAATG TGGTGACTAC ATCCCAAATG 
GACGAGCTGG CTAGGGTTCT GGTCACCCTA TTCGACGCCA AACACCTGTT ATCGCCACTC 
CTATGGAATA TGTTTTATCG CGAGGTGGAG GTCTCAGACT GCATGCAGAC ACTTTTCCGT 
GGAAATTCTT TGGGCAGCAA AATCATGGCC TTTTGCTTTA AAATATACGG CGCGAGCTAT 
CTCCAAATGC TACTAGAGCC TTTAATTCGT CCGCTTCTGG ATGAGGAGGA GGAGACCTGT 
TTTGAGGTGG ATCCAGCTCG TCTGGATCCA ACAGAAGATA TTGAACAGCA CAGGAACAAC 
TTGATTGCCC TAACGCAGAA GGTGTTTGAC GCTATTATCA ACTCGTCGGA TCGCTTTCCC 
CCGCAGCTGC GATCCATGTG CCATTGCCTG TACCAAGTGC TAAGCAAACG CTTCCCGAAT 
CTTCTTCAAA ACAATATCGG TGCTGTAGGC ACAGTCATCT TCTTGCGGTT CATCAATCCC 
GCCATAGTTT CCCCACAGGA ATTGGGAATC GTTGACAAAC AAGTGCACAG CTCGGCCAAA 
CGAGGTCTAA TGCTAATGTC CAAGATCCTT CAAAACATTG CTAACCACGT GGAGTTCTCC 
AAGGAGCAGC ACATGTTGTG TTTCAACGAT TTTCTGCGCG ATCACTTTGA AGCTGGGCGG 
CGATTCTTCA TACAGATTGC ATCAGACTGT GAGACCGTGG ATCAGACCTC GCACAGCATG 
AGTTTTATTT CAGATGCGAA CGTACTGGCA CTGCATCGGT TGCTGTGGAC GCATCAGGAG 
AAGATCGGCG ACTATCTGTC TAGCAGTCGA GACCACAAGG CGGTTGGAAG GCGGCCCTTC 
GACAAGATGG CTACTTTGCT AGCATACCTG GGACCACCGG AGCACAAGCC CGTGGATTCG 
CACATGATGT TCAGCTCGTA TGCACGCTGG AGCTCCATTG ACATGTCGTC GACAAACTTC 
GAGGAGATCA TGGTCAAGCA CCAAATGCAC GAGAAGGAGG AATTCAAGAC CCTCAAGTCG 
ATGAACATAT TCTACCAGGC CGGAACAAGC AAATCTGGCT ATCCTGTCTT TTACTATATA 
GCAAGACGAT ACAAGATTGG AGAAACGAAT GGAGACTTAC TTATATATCA CGTCATACTC 
ACTCTGAAAC CATTCTGCCA CTCGCCCTTC GAGGTGGTCA TCGATTTTAC GCACACCTGT 
TCAGATAATC GGTTCCGCAC CGAGTTTCTG CAGAAGTGGT TTTATGTCCT GCCCACGGTA 
GCCTACGAAA ATGTCCATGC TGTGTACATC TATAACTGTA ACTCGTGGGT GCGCGAATAT 
ACCAAGTTCC ACGATCGCAT TCTGGCACCA TTAAAGGGAA ATCGCAAACT TCTGTTCCTG 
GAGTCACCCA ACAAACTTAC TGATTTCATC GACGCGGAGC AACAGAAATT GCCTGGAGCA 
ACTCTTTCCT TGGACGAAGA TTTAAAGGTA TTCAGCAACG CGCTGAAGCT CAGCCATAAA 
GACACAAAGG TGGCTATCAA AGTGGGTCCC ACCGCATTGC AAATTACATC TGCCGAAAAG 
ACAAAGGTCT TGGCTCACTC CGTGCTCCTG AACGATGTAT ACTACGCCTC TGAAATTGAG 
GAGGTGTGCT TGGTGGACGA TAACCAGTTC ACCCTGTCTA TAACTAACGA AAGTGGCCAG 
CTTAGCTTTA TTCACAACGA TTGCGACAAC ATTGTTCAAG CCATCATACA CATCCGAAAT 
CGATGGGAGC TTAGTCAGCC GGACTCCGTG ACGGTTCACC AAAAGATCCG ACCAAAAGAT 
GTGCCTGGAA CGCTGCTGAA CATGGCGCTA CTCAATCTGG GATCATGTGA CCCCAATCTA 
AGGACTGCTG CCTACAATTT GCTGTGTGCT TTGACCGCTA CTTTTGATCT AAAGATTGAG 
GGTCAACTGT TGGAGACCCA AGGACTTTGC ATACCCTCAA ATAATACCAT ATTTATCAAA 
TCCGTTAGCG AAAAGCTGGC CACCAATGAA CCGCATCTGA CTCTGGAATT CCTTGAGGAG 
TCCATTCAGG GCTTCCAGCG CAGCACCATA GAGCTGAAAC ATTTGTGTTT GGAGTACATG 
ACGCCATGGT TAAAGAACCT AGTCAAATTT TGCAAGTCCA ACGATGACTC CAAAAAGCTT 
AAGGTCTCCC AAATTCTGGA CAAGCTCATC AATTTAACTA TTGACCAAAA GGAAATGTAT 
CCCTCAGTGC AGGCCAAGAT CTGGGGATCA ATTGGACAGA TCCCCGAGCT GATCGACATG 
GTTTTGGATA ACTTTTTGCA CAAATCGATC ACATATGGCT TGGGATCACC ACAGGTGGAG 
ATTATGGCTG ACACGGCAGT GGCTCTCGCT TCAGCGAATG TTCAGCTGGT GTCCAAAAAG 
GTCATAACGC GAATATGCCG AGTCATGGAC AAATCCTGCA CAAATCCCAC ACAATATCTG 
GAGCAGCATA TGATGTGGGA CGATATTGCA ATTCTTGGTC GATACCTGCT CATGTTGTCG 
TTCAACAATT GTTTGGATGT GGCCACATCG GTGCCATATC TATTCCACAC CATTACGTTT 
TTGGTATGTT CAGGATCCCT GTCGATGCGA GCCTCTACTC ATGGCCTAGT GATCAACATC 
ATCCACTCGC TGTGCACATG CACAAATCCC TCATTTTCAG AGGAGGCGCA GCGAGTACTC 
CGACTGTCCC TGGATGAATT CTCACTGCCC AAGTTCTATC TACTCTTCGG CATCAGCAAG 
GTCAAGTCCG CAGCAGTTAC CGCTTTCCGC TCCAGCTGCC GTCACCCTAC AGATAAATGG 
CTAGGAAATG AAAGGGTTAC CCAGCCTCTG CCCGCCGATC GAGAGCGTTT ATCCCTACCA 
TCGCTTGAGG TTATCACGGA TGCCCTGCTG GAAATCATGG AGGCTTGCAT GCGAGATGTT 
CCGGACTGCG AATGGCTCAA CACCTGGACC TCTTTGGCTC GCAGTTTTGC TTTCTGTTAC 
AATCCAGCGC TACAGCCTAG AGCCCTTATA GTTTACGGGT GCATCAGCAA GAGTGTTACC 
GATCACGAGG TCAAGCAGTT ACTACGCATC CTGGTTAAGG CCCTCGAATC TTTCAACGAT 
CTCATTCTGA TCGAGGCTCT AGTAATGTGC TTAACTCGCA TTCAACCACT ACTCCGACCA 
GAGTCGCCCA TTCATCGGGC CCTCTTCTGG GTGGCCATTT CGGTGCTGCA GTTGGACGAG 
ATTACCCTGT ACGGCGCTGG ACTAGCTCTG CTTGAACAAA ATCTACACAC GCTTAAGTCA 
CAGGGCTGTT TTGACAAGAA GGAGACCATA GCCGAGGTCA TGATGAAGAC AAGGGAAAAG 
CTGGAGTGGC ATTTCAAGCA ACTGGATCAC GCCGTCGGCC TCTCCTTCCG CAGCAATTTC 
CACTTTGCCC TAGTGGGACA CTTGATTAAG GGCTTCCGCC ATCCTACACC TACAACCGTC 
TCACGCACCT CTCGTGTGCT GACGATGCTC TTGGGCATAA TAGCCAAACC CCTGCATCGG 
GACAAGTTCG AAGTAACACC TGACAGTGTG GCCTATTTGA CTGCGCTGGT GGCTGTCTCC 
GAGGAAGTCC GTTCCAGATG TCACGTAAAA CATGCCCTTC CCCGCTGGCC AGCCGATCTG 
AGTAGTAGTG TGGAAAATGG TGAAGCATCC GGCGGAGTGC AAGCTATCGG CCTGCCCCTG 
TCACGCCGTC AAAAAAGTTG GGACATCCTG GATCAGTCCG CCTTGCAGTT TGCCCGCCAA 
CACAAGGTTC CCACTCTTCA GGAACGCGGT TCGCGTTCGT CGGTGTCCAA CGAGTCCAAC 
GTACTGCTCG ATCCCGAGGT CTTACCTGAC CTGTCCATTC AAGCCCTGGT ACTGACTGTT 
TTGGCCACCT TGGTCAAGTA CTCTTCGGAT GAAGGCGAAA CGCGGGTTTT GTATCAGTAT 
TTGGCCGAAG GATCGGTGGT CTTTCCAAAG GTGTTTCCAG TTATCCATTC ACTGCTGGAC 
CAAAAGATAA ACAACATTTT GTCAGTGTCA CACGACCAAG TGGTGCTCAA CTCTGTGCAG 
AACATTATAC AAAATATGCT GGCCAGCGAA GATCCTTCCC AGCAGCAGCT GCACTTCCTG 
CAGAGTTGTG GATTTGGCGG ACTCTGGCGA TTTGCTGGTC CCTTCACCAA GTACAATATG 
ATGGGTGAGT CCTCGGAGCT GTTTGTCAAC TGTTTGGAGG CCATGGTTGA GACCTGTTTG 
CCAGGCGACG AATCCGCGCC GGTGCCACCA TCTCCGCGTC CATACAACCT GAGCTCCAGT 
CTGAGTAGCC TGACCCTGGG ATCACCCACG GATAAAGCTG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nf1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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