tobi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	tobi
Entrez Gene ID	43072
Full Name	target of brain insulin
Synonyms	CG11909,CT33098,Dmel\CG11909
General protein information	Preferred Names target of brain insulin Names CG11909 gene product from transcript CG11909-RA CG11909-PA alpha-Glucosidase tobi-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96D2-96D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_143134.3	NP_651391.1	target of brain insulin

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	si:ch211-117c19.1	XP_683887.3
Drosophila melanogaster (fruit fly)	tobi	NP_651391.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003995	XP_318449.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tobi gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tobi cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06090	NM_143134.3 Latest version!	Drosophila melanogaster target of brain insulin (tobi), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tobi ( NM_143134.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651391.1 Drosophila melanogaster target of brain insulin (tobi), mRNA.

Price & Availability↑

CloneID	OFa06090
Clone ID Related Accession (Same CDS sequence)	NM_143134.3
Accession Version	NM_143134.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	target of brain insulin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143134.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGGTTTA TTTTACTATC CCTGCTGTTG GCATCCTTCT GCCTCTGGGA GGCGGTTCAC 
ACTTGTGCCA GCATCGAGCA GCGCTACAAG TTTCCCAATA GCAATGTCTA CGTGCGACTT 
AGGAGAGGTG ATAAGTTGCA GTATGAGCTA ATGAAGGGCG AGAAGTCCCT GCAGACGGTG 
ACCTTCGACA ACTTTGACAG CAGCGCCAAT CTGGTGGAGA CGAGCAGTGG TAGCTACCAG 
CTTACCGATG GCCAATCCAC CGTGGAGTTC ACCCTGGTCA AAAGCGGAGT GGTTGCCGGC 
ACAGGCAGTG AGATAACCCA TGTGAAAGTA TCCCGAGATC AGGTGCTGCA AGGCACCCAA 
CCCAGGGATT GCGTCCCCCT GAAGACCGGA TCGACGCACT GGTTCAGTGG TCCGGAGCAG 
AAGCAACACT ACTGGCCAGT GGAGCGACAG AGGCATAGTA ACTATTCCTA CCTGCCAAAG 
GAGCTGGATA ACTTTGGTGT GGGTGAGCGA TATTGGTTGA ACGGCGACGG AGTCTTTTTG 
TACGTGGAGA GCAACACTCC TCTGTTTATT GATCAGAATA GTGCTGCCTA TCCTGACCAG 
CTGTGTCTGA GTGCTATTAG TGCTCTACCC TATGATCCTC GGGTGACCTC TGCCAAATTC 
GTTTACCACG TAGCTGTGGC CAAGGATGCC AAGGCGGCGC ACCGGTATGC CGTTAAAACG 
TTCCTCGGCC AACCCACAGG CTATCCCGAT GAGCGAATGG TCATGCATCC TGTGTGGTCC 
ACATGGGCTC TCTACAAGGC TGAGGTATCC GACGATGTGG TTCGTGACTT CGCCAAGCAG 
ATTCTGGATA ATGGGTTCAA CAACAGCCAG CTGGAAATCG ACGATGATTG GGAGGATTGC 
TATGGAGCCA TGACCTTCAG GAAGAACAAG TTCCCCGATA GCAAGACCCT GACTGATGAC 
CTCAAGGCCT TGGGTTTCCG TGTGACCCTG TGGATTCATC CATTCATTAA CAACAACTGC 
ACAGCCATTT ACGATGAAGC TAAGTCTAAG GGTTACCTGG TGCTCGATCA CGAGGGAAGC 
TCCGACACGC AATGGTGGAA CAGCAAGCCC AAGGATGCAG CCATTTTGGA CTTTACCAAA 
ACTGAGGTTC AGGAGTGGTT CAGCAAGCGT TTAAAGCGCC TTCAGGATGA GGATGGCATT 
GATAGCTTCA AGTTTGATGC CGGAGAGACC AGTTGGCTAC CCAGTGATCC TGTACTCCAG 
GCTAGCAGTT CCATGGTGAC ACCACTGCAG GCGGTGGGCG ACTACGTGCG TACTGTAGCG 
GCATTCGGAG ACATGGTGGA GGTTCGATCA GCCCAGAACA CTCAAGATCA GCCAATCTTC 
GTGCGCATCG TGGACAAGGA CTCCGAATGG GGATGGAACA ACGGCCTGCT CACACTGATC 
TCTTCGATGC TGCAAATGAA CCTCAACGGT TATCCTTTCG TCCTTCCCGA TATGATTGGT 
GGTAATGGCT ACTACGAAAA GCCACCAACC AAGGAGCTTT TCCTCCGTTG GCTGCAGGCC 
AACGTCTTTA TGCCCGCCCT GCAGTTCTCC TTCGTTCCCT GGAACTTTGA CGACGAGGCC 
ATTGCGATCA GCAAGAACTT CACCGCCCTC CATGCCACCT ATACACCTTA CATTATGAAG 
CTCTTTAAGC GCGCTGTGGA TTCTGGTGAA CCTGTGAATG TTCCCCTCTG GTGGATCGCC 
CCGACTGATG AGGTGGCGCA GTCTATCTAT GATGAGTTCC TTTTGGGCGA AGACATCATC 
GCTGCTCCCG TTGTGGTCGA GGGCGCCACC AAGCGAGACA TTTACCTGCC AGAGGGCGAG 
TGGCAGGATG GAAACAGCGA CCAGGTGTAC ACTGGACCCA CCTGGGTGAT GGACTACGCC 
GCTCCCTTGG ATACCCTACC CTACTTTTTG CGCGTGGGAT TCACACTGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651391.1
CDS	36..2009
Translation	MRFILLSLLLASFCLWEAVHTCASIEQRYKFPNSNVYVRLRRGDKLQYELMKGEKSLQTVTFDNFDSSANLVETSSGSYQLTDGQSTVEFTLVKSGVVAGTGSEITHVKVSRDQVLQGTQPRDCVPLKTGSTHWFSGPEQKQHYWPVERQRHSNYSYLPKELDNFGVGERYWLNGDGVFLYVESNTPLFIDQNSAAYPDQLCLSAISALPYDPRVTSAKFVYHVAVAKDAKAAHRYAVKTFLGQPTGYPDERMVMHPVWSTWALYKAEVSDDVVRDFAKQILDNGFNNSQLEIDDDWEDCYGAMTFRKNKFPDSKTLTDDLKALGFRVTLWIHPFINNNCTAIYDEAKSKGYLVLDHEGSSDTQWWNSKPKDAAILDFTKTEVQEWFSKRLKRLQDEDGIDSFKFDAGETSWLPSDPVLQASSSMVTPLQAVGDYVRTVAAFGDMVEVRSAQNTQDQPIFVRIVDKDSEWGWNNGLLTLISSMLQMNLNGYPFVLPDMIGGNGYYEKPPTKELFLRWLQANVFMPALQFSFVPWNFDDEAIAISKNFTALHATYTPYIMKLFKRAVDSGEPVNVPLWWIAPTDEVAQSIYDEFLLGEDIIAAPVVVEGATKRDIYLPEGEWQDGNSDQVYTGPTWVMDYAAPLDTLPYFLRVGFTLE

Target ORF information:

RefSeq Version	NM_143134.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster target of brain insulin (tobi), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143134.3

ORF Insert Sequence:

ATGAGGTTTA TTTTACTATC CCTGCTGTTG GCATCCTTCT GCCTCTGGGA GGCGGTTCAC 
ACTTGTGCCA GCATCGAGCA GCGCTACAAG TTTCCCAATA GCAATGTCTA CGTGCGACTT 
AGGAGAGGTG ATAAGTTGCA GTATGAGCTA ATGAAGGGCG AGAAGTCCCT GCAGACGGTG 
ACCTTCGACA ACTTTGACAG CAGCGCCAAT CTGGTGGAGA CGAGCAGTGG TAGCTACCAG 
CTTACCGATG GCCAATCCAC CGTGGAGTTC ACCCTGGTCA AAAGCGGAGT GGTTGCCGGC 
ACAGGCAGTG AGATAACCCA TGTGAAAGTA TCCCGAGATC AGGTGCTGCA AGGCACCCAA 
CCCAGGGATT GCGTCCCCCT GAAGACCGGA TCGACGCACT GGTTCAGTGG TCCGGAGCAG 
AAGCAACACT ACTGGCCAGT GGAGCGACAG AGGCATAGTA ACTATTCCTA CCTGCCAAAG 
GAGCTGGATA ACTTTGGTGT GGGTGAGCGA TATTGGTTGA ACGGCGACGG AGTCTTTTTG 
TACGTGGAGA GCAACACTCC TCTGTTTATT GATCAGAATA GTGCTGCCTA TCCTGACCAG 
CTGTGTCTGA GTGCTATTAG TGCTCTACCC TATGATCCTC GGGTGACCTC TGCCAAATTC 
GTTTACCACG TAGCTGTGGC CAAGGATGCC AAGGCGGCGC ACCGGTATGC CGTTAAAACG 
TTCCTCGGCC AACCCACAGG CTATCCCGAT GAGCGAATGG TCATGCATCC TGTGTGGTCC 
ACATGGGCTC TCTACAAGGC TGAGGTATCC GACGATGTGG TTCGTGACTT CGCCAAGCAG 
ATTCTGGATA ATGGGTTCAA CAACAGCCAG CTGGAAATCG ACGATGATTG GGAGGATTGC 
TATGGAGCCA TGACCTTCAG GAAGAACAAG TTCCCCGATA GCAAGACCCT GACTGATGAC 
CTCAAGGCCT TGGGTTTCCG TGTGACCCTG TGGATTCATC CATTCATTAA CAACAACTGC 
ACAGCCATTT ACGATGAAGC TAAGTCTAAG GGTTACCTGG TGCTCGATCA CGAGGGAAGC 
TCCGACACGC AATGGTGGAA CAGCAAGCCC AAGGATGCAG CCATTTTGGA CTTTACCAAA 
ACTGAGGTTC AGGAGTGGTT CAGCAAGCGT TTAAAGCGCC TTCAGGATGA GGATGGCATT 
GATAGCTTCA AGTTTGATGC CGGAGAGACC AGTTGGCTAC CCAGTGATCC TGTACTCCAG 
GCTAGCAGTT CCATGGTGAC ACCACTGCAG GCGGTGGGCG ACTACGTGCG TACTGTAGCG 
GCATTCGGAG ACATGGTGGA GGTTCGATCA GCCCAGAACA CTCAAGATCA GCCAATCTTC 
GTGCGCATCG TGGACAAGGA CTCCGAATGG GGATGGAACA ACGGCCTGCT CACACTGATC 
TCTTCGATGC TGCAAATGAA CCTCAACGGT TATCCTTTCG TCCTTCCCGA TATGATTGGT 
GGTAATGGCT ACTACGAAAA GCCACCAACC AAGGAGCTTT TCCTCCGTTG GCTGCAGGCC 
AACGTCTTTA TGCCCGCCCT GCAGTTCTCC TTCGTTCCCT GGAACTTTGA CGACGAGGCC 
ATTGCGATCA GCAAGAACTT CACCGCCCTC CATGCCACCT ATACACCTTA CATTATGAAG 
CTCTTTAAGC GCGCTGTGGA TTCTGGTGAA CCTGTGAATG TTCCCCTCTG GTGGATCGCC 
CCGACTGATG AGGTGGCGCA GTCTATCTAT GATGAGTTCC TTTTGGGCGA AGACATCATC 
GCTGCTCCCG TTGTGGTCGA GGGCGCCACC AAGCGAGACA TTTACCTGCC AGAGGGCGAG 
TGGCAGGATG GAAACAGCGA CCAGGTGTAC ACTGGACCCA CCTGGGTGAT GGACTACGCC 
GCTCCCTTGG ATACCCTACC CTACTTTTTG CGCGTGGGAT TCACACTGGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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tobi cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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