ssh cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ssh
Entrez Gene ID	42986
Full Name	slingshot
Synonyms	CG6238,Dmel\CG6238,MKP,Mkph,SSH,Ssh,l(3)01207
General protein information	Preferred Names slingshot Names CG6238 gene product from transcript CG6238-RA CG6238-PA CG6238-PB CG6238-PC CG6238-PD MAP-kinase-phosphatase MKP-like ssh-PA ssh-PB ssh-PC ssh-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96B10-96B11

mRNA and Protein(s)

mRNA	Protein	Name
NM_079768.4	NP_524492.2	slingshot, isoform A
NM_170184.2	NP_733063.1	slingshot, isoform B
NM_001170245.1	NP_001163716.1	slingshot, isoform C
NM_001170246.1	NP_001163717.1	slingshot, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In mature neurons SSH may function as a stabilization factor for filamentous actin instead of promoting actin depolymerization.
Title: Increased actin polymerization and stabilization interferes with neuronal function and survival in the AMPK? mutant Loechrig.

we found that Capt(Capulet) and Ssh(Slingshot), but not Tsr, share overlapping functions during eye morphogenesis
Title: Capulet and Slingshot share overlapping functions during Drosophila eye morphogenesis.

Ssh acts in the developing eye to limit actin polymerization in the assembling ommatidia, but not in the morphogenetic furrow.
Title: Slingshot cofilin phosphatase localization is regulated by receptor tyrosine kinases and regulates cytoskeletal structure in the developing Drosophila eye.

The following ssh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ssh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06049	NM_001170246.1 Latest version!	Drosophila melanogaster slingshot (ssh), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa06050	NM_001170245.1 Latest version!	Drosophila melanogaster slingshot (ssh), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa08780	NM_170184.2 Latest version!	Drosophila melanogaster slingshot (ssh), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa08819	NM_079768.4 Latest version!	Drosophila melanogaster slingshot (ssh), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ssh ( NM_001170246.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163717.1 Drosophila melanogaster slingshot (ssh), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa06049
Clone ID Related Accession (Same CDS sequence)	NM_001170246.1
Accession Version	NM_001170246.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3138bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	slingshot, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCTGG TGACGGTGCA GCGCTCTCCC AGTGTGGCGG GCTCCTGCTC GAATTCGGAT 
GGCGAGTCCG AGGACGACGA GGGCAATAGC AAAGGAAACG ATCGCAGCGA GTGCTTCTTT 
GCCGGCAAGG GAACCGCCCT GGTACTGGCT CTTAAGGATA TCCCGCCCCT GACGCAAAGC 
GAGCGTCGCC TGAGCACCGA CTCCACCCGC TCCAGTAACA GTACGCAAAG CAACAACTCG 
GACATCCAGC TCCACCTGCA ATCAATGTTT TACCTGTTGC AGCGCGAGGA CACGCTAAAG 
ATGGCTGTCA AGTTGGAGTC ACAGCGGTCC AACCGCACGC GCTATCTGGT GATTGCATCG 
CGTAGTTGTT GTCGGAGCGG CACCAGCGAC CGTCGGCGCC ACCGGATCAT GCGTCACCAC 
TCGGTAAAAG TTGGTGGATC CGCGGGAACA AAGTCGTCCA CCTCCCCGGC GGTCCCCACG 
CAGCGTCAGC TGTCCGTTGA GCAGACCGCC ACAGAGGCCA GCAGCAAATG TGATAAAACA 
GCAGACAAGG AAAACGCAAC GGCGGCCGGC GACAACAAGA ATACCTCGGG CATGGAGGAG 
TCCTGCCTGC TGGGCATAGA CTGCAACGAG CGGACCACCA TTGGCCTGGT GGTGCCCATC 
CTGGCGGACA CCACAATTCA CCTGGATGGC GATGGCGGAT TCAGCGTCAA AGTGTACGAA 
AAAACGCATA TATTTAAGCC GGTCTCAGTG CAGGCCATGT GGTCCGCGCT GCAGACGCTG 
CACAAAGTGT CGAAAAAGGC TCGTGAGAAC AACTTCTATG CCAGTGGACC CTCCCACGAC 
TGGCTATCCA GCTACGAGAG GCGCATCGAG TCGGACCAGT CTTGTCTGAA CGAGTGGAAT 
GCCATGGACG CGCTCGAGAG CCGCCGACCA CCTTCGCCTG ATGCCATACG AAACAAACCC 
CCCGAAAAGG AGGAAACCGA AAGTGTCATT AAGATGAAGC TGAAGGCCAT CATGATGTCA 
GTGGACCTGG ACGAGGTCAC CTCCAAGTAT ATACGCGGAC GCCTCGAGGA GATCCTGGAC 
ATGGATTTGG GCGAGTACAA GTCGTTTATC GACGCCGAAA TGCTCGTTAT TCTAGGCCAA 
ATGGATGCCC CCACCAAGAT TTTCGAGCAC GTCTATCTGG GATCAGAGTG GAATGCCAGC 
AACTTGGAGG AGCTGCAGAA AAACGGCGTT CGCCACATCC TGAACGTGAC GCGGGAGATC 
GATAACTTCT TTCCGGGCAC CTTCGAGTAT TTCAACGTTC GCGTGTACGA CGATGAGAAG 
ACGAACCTGC TCAAGTACTG GGACGATACG TTCCGCTACA TCACGCGGGC GAAAGCCGAG 
GGATCCAAGG TGCTGGTGCA CTGCAAGATG GGCGTCAGCC GATCCGCCTC GGTTGTCATC 
GCATATGCCA TGAAGGCTTA CCAGTGGGAG TTCCAGCAGG CGCTAGAGCA CGTGAAGAAG 
AGGCGCAGCT GTATAAAGCC CAACAAGAAC TTCCTCAATC AGCTGGAGAC GTATAGTGGG 
ATGTTGGACG CCATGAAGAA CAAGGAGAAG CTGCAGCGCA GCAAGAGCGA GACGAACTTG 
AAGAGCACAA AGGACGCGCG ACTGTTGCCT GGTAGCGAAC CAACGCCCCT CATTCAGGCT 
CTAAACCAGG CCAAGTCGAA GTCGACTGGT GAAGCGGGCG TAACACCCGA TGGCGAAGAG 
GAGGATGGTT CACGCATGCA CCGGCGATCC ATCGCCCAGA AGTCACAGCG TCGCATGGTT 
AGGCGCTCGA GCAGCACATC ACCCAAAACC CAGACGGCTG TGGTTACCAA GCAGCAAAGC 
CAGTCTATGG AGAACCTTAC GCCGGAACGT AGCGTGGCCG AGGAGCCAAA AAATATGCGC 
TTCCCAGGCA GCAACGGCGA GAACTACAGC GTCACCCAGA ACCAGGTGCT GCACATCCAG 
AAACACACGC CTCTGTCAGT GCGCACACGA ATTCACGATC TCGAGGCGCA CCGGGCGGAC 
CAGCTGCCAC AGCAACCTGT CTGGACCTCG CTGACAAAAC TGATTACACA GACCTCTCAC 
TTGGGAAAAA GTGTCAGTGG CAGCAGTAGT GGAAATATTG ACTCGCGGCG GGATTCTAGC 
TGTTCGGATG TATTCTCCTC GCAGGTGGAC AGTGTTTTTG CCAAGGATGA AGGGGAGAAG 
CGCCAGCGTC GAAAGACGCA CTCCTGGACA GAGTCCTTGG GTCCAAGTGG CGGCATCGTC 
CTGGACCCCA CGCCACAGCA ACAGAAGCAG CAGTCAAACG CCATTCTGCG TCCCCGAGGC 
ACACGTCAGC GGGAGCTGCC TTCGCGGCAC GCAAGTTGGG GATCAGGAGA CAACCGATGC 
TGTCTGCCGC AGAGGACCAG TTCCGGCTCC TACTATGACT CCAATCGCAA CACTACGGCC 
ATCTTCGAGG GCGTTATCCA AGACCTCAAG CGCAGTTCCA ATTGCAATGT AATCGAAGGA 
GTCGCCGTTG CTGTGGAACC GATAGTTGGA GTTGGCGAGG GCACCGTAAA GCGCACCAAG 
CAGAAGCTGG AAGAGAGCAC CTCGCTGAAA AAGCGCTGCC AGGAAGAAAG CCAGGAGCTG 
CTACTCGAGG CTGTGGATGC TGGGCAGAGG CGGTGCCCAA GCCTCTATAG GAGCGCCTCC 
TCGGCGCACA GTCCGCGGCA GCGTCAGCCA CTACGCTGTA ATAGTGAGGA GCTGATGCAC 
ACCAGCGACA TCGAGAACAA GAACACCACG CCAGGCGACC ATGAGGCGAC GGTAGTGCTG 
CGCGTCCAGG GACAAACGCT GGCCGACGAT CAGCGCATCT CGGGCAATGT GCAAATACTC 
AAGCAAAATT TCGAAGCCAA AGCGGGAGTG GTGGGCACAG GAGGAGGAGG AGGCGGTGGC 
ACTGCAGCGG GATCGGGCAG TAGTTCAACA ATCGCAACGT CGGTATCGGC GGTGGCCGGA 
GCCTGCTCAG CGAATCCCGG CAAGAAGACG ACTAGCCACC AATCACTGCC ATCGTCGCCG 
GTGGCGCAGC ATGCCAACCA CGTGTCCGCC GCCTCCAACT CCAACTCCTC CGCCTCCAAC 
TCGTCCGATT CCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163717.1
CDS	483..3620
Translation	MALVTVQRSPSVAGSCSNSDGESEDDEGNSKGNDRSECFFAGKGTALVLALKDIPPLTQSERRLSTDSTRSSNSTQSNNSDIQLHLQSMFYLLQREDTLKMAVKLESQRSNRTRYLVIASRSCCRSGTSDRRRHRIMRHHSVKVGGSAGTKSSTSPAVPTQRQLSVEQTATEASSKCDKTADKENATAAGDNKNTSGMEESCLLGIDCNERTTIGLVVPILADTTIHLDGDGGFSVKVYEKTHIFKPVSVQAMWSALQTLHKVSKKARENNFYASGPSHDWLSSYERRIESDQSCLNEWNAMDALESRRPPSPDAIRNKPPEKEETESVIKMKLKAIMMSVDLDEVTSKYIRGRLEEILDMDLGEYKSFIDAEMLVILGQMDAPTKIFEHVYLGSEWNASNLEELQKNGVRHILNVTREIDNFFPGTFEYFNVRVYDDEKTNLLKYWDDTFRYITRAKAEGSKVLVHCKMGVSRSASVVIAYAMKAYQWEFQQALEHVKKRRSCIKPNKNFLNQLETYSGMLDAMKNKEKLQRSKSETNLKSTKDARLLPGSEPTPLIQALNQAKSKSTGEAGVTPDGEEEDGSRMHRRSIAQKSQRRMVRRSSSTSPKTQTAVVTKQQSQSMENLTPERSVAEEPKNMRFPGSNGENYSVTQNQVLHIQKHTPLSVRTRIHDLEAHRADQLPQQPVWTSLTKLITQTSHLGKSVSGSSSGNIDSRRDSSCSDVFSSQVDSVFAKDEGEKRQRRKTHSWTESLGPSGGIVLDPTPQQQKQQSNAILRPRGTRQRELPSRHASWGSGDNRCCLPQRTSSGSYYDSNRNTTAIFEGVIQDLKRSSNCNVIEGVAVAVEPIVGVGEGTVKRTKQKLEESTSLKKRCQEESQELLLEAVDAGQRRCPSLYRSASSAHSPRQRQPLRCNSEELMHTSDIENKNTTPGDHEATVVLRVQGQTLADDQRISGNVQILKQNFEAKAGVVGTGGGGGGGTAAGSGSSSTIATSVSAVAGACSANPGKKTTSHQSLPSSPVAQHANHVSAASNSNSSASNSSDSS

Target ORF information:

RefSeq Version	NM_001170246.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster slingshot (ssh), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170246.1

ORF Insert Sequence:

ATGGCGCTGG TGACGGTGCA GCGCTCTCCC AGTGTGGCGG GCTCCTGCTC GAATTCGGAT 
GGCGAGTCCG AGGACGACGA GGGCAATAGC AAAGGAAACG ATCGCAGCGA GTGCTTCTTT 
GCCGGCAAGG GAACCGCCCT GGTACTGGCT CTTAAGGATA TCCCGCCCCT GACGCAAAGC 
GAGCGTCGCC TGAGCACCGA CTCCACCCGC TCCAGTAACA GTACGCAAAG CAACAACTCG 
GACATCCAGC TCCACCTGCA ATCAATGTTT TACCTGTTGC AGCGCGAGGA CACGCTAAAG 
ATGGCTGTCA AGTTGGAGTC ACAGCGGTCC AACCGCACGC GCTATCTGGT GATTGCATCG 
CGTAGTTGTT GTCGGAGCGG CACCAGCGAC CGTCGGCGCC ACCGGATCAT GCGTCACCAC 
TCGGTAAAAG TTGGTGGATC CGCGGGAACA AAGTCGTCCA CCTCCCCGGC GGTCCCCACG 
CAGCGTCAGC TGTCCGTTGA GCAGACCGCC ACAGAGGCCA GCAGCAAATG TGATAAAACA 
GCAGACAAGG AAAACGCAAC GGCGGCCGGC GACAACAAGA ATACCTCGGG CATGGAGGAG 
TCCTGCCTGC TGGGCATAGA CTGCAACGAG CGGACCACCA TTGGCCTGGT GGTGCCCATC 
CTGGCGGACA CCACAATTCA CCTGGATGGC GATGGCGGAT TCAGCGTCAA AGTGTACGAA 
AAAACGCATA TATTTAAGCC GGTCTCAGTG CAGGCCATGT GGTCCGCGCT GCAGACGCTG 
CACAAAGTGT CGAAAAAGGC TCGTGAGAAC AACTTCTATG CCAGTGGACC CTCCCACGAC 
TGGCTATCCA GCTACGAGAG GCGCATCGAG TCGGACCAGT CTTGTCTGAA CGAGTGGAAT 
GCCATGGACG CGCTCGAGAG CCGCCGACCA CCTTCGCCTG ATGCCATACG AAACAAACCC 
CCCGAAAAGG AGGAAACCGA AAGTGTCATT AAGATGAAGC TGAAGGCCAT CATGATGTCA 
GTGGACCTGG ACGAGGTCAC CTCCAAGTAT ATACGCGGAC GCCTCGAGGA GATCCTGGAC 
ATGGATTTGG GCGAGTACAA GTCGTTTATC GACGCCGAAA TGCTCGTTAT TCTAGGCCAA 
ATGGATGCCC CCACCAAGAT TTTCGAGCAC GTCTATCTGG GATCAGAGTG GAATGCCAGC 
AACTTGGAGG AGCTGCAGAA AAACGGCGTT CGCCACATCC TGAACGTGAC GCGGGAGATC 
GATAACTTCT TTCCGGGCAC CTTCGAGTAT TTCAACGTTC GCGTGTACGA CGATGAGAAG 
ACGAACCTGC TCAAGTACTG GGACGATACG TTCCGCTACA TCACGCGGGC GAAAGCCGAG 
GGATCCAAGG TGCTGGTGCA CTGCAAGATG GGCGTCAGCC GATCCGCCTC GGTTGTCATC 
GCATATGCCA TGAAGGCTTA CCAGTGGGAG TTCCAGCAGG CGCTAGAGCA CGTGAAGAAG 
AGGCGCAGCT GTATAAAGCC CAACAAGAAC TTCCTCAATC AGCTGGAGAC GTATAGTGGG 
ATGTTGGACG CCATGAAGAA CAAGGAGAAG CTGCAGCGCA GCAAGAGCGA GACGAACTTG 
AAGAGCACAA AGGACGCGCG ACTGTTGCCT GGTAGCGAAC CAACGCCCCT CATTCAGGCT 
CTAAACCAGG CCAAGTCGAA GTCGACTGGT GAAGCGGGCG TAACACCCGA TGGCGAAGAG 
GAGGATGGTT CACGCATGCA CCGGCGATCC ATCGCCCAGA AGTCACAGCG TCGCATGGTT 
AGGCGCTCGA GCAGCACATC ACCCAAAACC CAGACGGCTG TGGTTACCAA GCAGCAAAGC 
CAGTCTATGG AGAACCTTAC GCCGGAACGT AGCGTGGCCG AGGAGCCAAA AAATATGCGC 
TTCCCAGGCA GCAACGGCGA GAACTACAGC GTCACCCAGA ACCAGGTGCT GCACATCCAG 
AAACACACGC CTCTGTCAGT GCGCACACGA ATTCACGATC TCGAGGCGCA CCGGGCGGAC 
CAGCTGCCAC AGCAACCTGT CTGGACCTCG CTGACAAAAC TGATTACACA GACCTCTCAC 
TTGGGAAAAA GTGTCAGTGG CAGCAGTAGT GGAAATATTG ACTCGCGGCG GGATTCTAGC 
TGTTCGGATG TATTCTCCTC GCAGGTGGAC AGTGTTTTTG CCAAGGATGA AGGGGAGAAG 
CGCCAGCGTC GAAAGACGCA CTCCTGGACA GAGTCCTTGG GTCCAAGTGG CGGCATCGTC 
CTGGACCCCA CGCCACAGCA ACAGAAGCAG CAGTCAAACG CCATTCTGCG TCCCCGAGGC 
ACACGTCAGC GGGAGCTGCC TTCGCGGCAC GCAAGTTGGG GATCAGGAGA CAACCGATGC 
TGTCTGCCGC AGAGGACCAG TTCCGGCTCC TACTATGACT CCAATCGCAA CACTACGGCC 
ATCTTCGAGG GCGTTATCCA AGACCTCAAG CGCAGTTCCA ATTGCAATGT AATCGAAGGA 
GTCGCCGTTG CTGTGGAACC GATAGTTGGA GTTGGCGAGG GCACCGTAAA GCGCACCAAG 
CAGAAGCTGG AAGAGAGCAC CTCGCTGAAA AAGCGCTGCC AGGAAGAAAG CCAGGAGCTG 
CTACTCGAGG CTGTGGATGC TGGGCAGAGG CGGTGCCCAA GCCTCTATAG GAGCGCCTCC 
TCGGCGCACA GTCCGCGGCA GCGTCAGCCA CTACGCTGTA ATAGTGAGGA GCTGATGCAC 
ACCAGCGACA TCGAGAACAA GAACACCACG CCAGGCGACC ATGAGGCGAC GGTAGTGCTG 
CGCGTCCAGG GACAAACGCT GGCCGACGAT CAGCGCATCT CGGGCAATGT GCAAATACTC 
AAGCAAAATT TCGAAGCCAA AGCGGGAGTG GTGGGCACAG GAGGAGGAGG AGGCGGTGGC 
ACTGCAGCGG GATCGGGCAG TAGTTCAACA ATCGCAACGT CGGTATCGGC GGTGGCCGGA 
GCCTGCTCAG CGAATCCCGG CAAGAAGACG ACTAGCCACC AATCACTGCC ATCGTCGCCG 
GTGGCGCAGC ATGCCAACCA CGTGTCCGCC GCCTCCAACT CCAACTCCTC CGCCTCCAAC 
TCGTCCGATT CCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ssh ( NM_001170245.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163716.1 Drosophila melanogaster slingshot (ssh), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa06050
Clone ID Related Accession (Same CDS sequence)	NM_001170245.1
Accession Version	NM_001170245.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	slingshot, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCATGC AAGTTTGTTC TTTGCCAGAC GACATCCTTA TATTTAGCCT AACCCCACGG 
GATGGCGAGT CCGAGGACGA CGAGGGCAAT AGCAAAGGAA ACGATCGCAG CGAGTGCTTC 
TTTGCCGGCA AGGGAACCGC CCTGGTACTG GCTCTTAAGG ATATCCCGCC CCTGACGCAA 
AGCGAGCGTC GCCTGAGCAC CGACTCCACC CGCTCCAGTA ACAGTACGCA AAGCAACAAC 
TCGGACATCC AGCTCCACCT GCAATCAATG TTTTACCTGT TGCAGCGCGA GGACACGCTA 
AAGATGGCTG TCAAGTTGGA GTCACAGCGG TCCAACCGCA CGCGCTATCT GGTGATTGCA 
TCGCGTAGTT GTTGTCGGAG CGGCACCAGC GACCGTCGGC GCCACCGGAT CATGCGTCAC 
CACTCGGTAA AAGTTGGTGG ATCCGCGGGA ACAAAGTCGT CCACCTCCCC GGCGGTCCCC 
ACGCAGCGTC AGCTGTCCGT TGAGCAGACC GCCACAGAGG CCAGCAGCAA ATGTGATAAA 
ACAGCAGACA AGGAAAACGC AACGGCGGCC GGCGACAACA AGAATACCTC GGGCATGGAG 
GAGTCCTGCC TGCTGGGCAT AGACTGCAAC GAGCGGACCA CCATTGGCCT GGTGGTGCCC 
ATCCTGGCGG ACACCACAAT TCACCTGGAT GGCGATGGCG GATTCAGCGT CAAAGTGTAC 
GAAAAAACGC ATATATTTAA GCCGGTCTCA GTGCAGGCCA TGTGGTCCGC GCTGCAGACG 
CTGCACAAAG TGTCGAAAAA GGCTCGTGAG AACAACTTCT ATGCCAGTGG ACCCTCCCAC 
GACTGGCTAT CCAGCTACGA GAGGCGCATC GAGTCGGACC AGTCTTGTCT GAACGAGTGG 
AATGCCATGG ACGCGCTCGA GAGCCGCCGA CCACCTTCGC CTGATGCCAT ACGAAACAAA 
CCCCCCGAAA AGGAGGAAAC CGAAAGTGTC ATTAAGATGA AGCTGAAGGC CATCATGATG 
TCAGTGGACC TGGACGAGGT CACCTCCAAG TATATACGCG GACGCCTCGA GGAGATCCTG 
GACATGGATT TGGGCGAGTA CAAGTCGTTT ATCGACGCCG AAATGCTCGT TATTCTAGGC 
CAAATGGATG CCCCCACCAA GATTTTCGAG CACGTCTATC TGGGATCAGA GTGGAATGCC 
AGCAACTTGG AGGAGCTGCA GAAAAACGGC GTTCGCCACA TCCTGAACGT GACGCGGGAG 
ATCGATAACT TCTTTCCGGG CACCTTCGAG TATTTCAACG TTCGCGTGTA CGACGATGAG 
AAGACGAACC TGCTCAAGTA CTGGGACGAT ACGTTCCGCT ACATCACGCG GGCGAAAGCC 
GAGGGATCCA AGGTGCTGGT GCACTGCAAG ATGGGCGTCA GCCGATCCGC CTCGGTTGTC 
ATCGCATATG CCATGAAGGC TTACCAGTGG GAGTTCCAGC AGGCGCTAGA GCACGTGAAG 
AAGAGGCGCA GCTGTATAAA GCCCAACAAG AACTTCCTCA ATCAGCTGGA GACGTATAGT 
GGGATGTTGG ACGCCATGAA GAACAAGGAG AAGCTGCAGC GCAGCAAGAG CGAGACGAAC 
TTGAAGAGCA CAAAGGACGC GCGACTGTTG CCTGGTAGCG AACCAACGCC CCTCATTCAG 
GCTCTAAACC AGGCCAAGTC GAAGTCGACT GGTGAAGCGG GCGTAACACC CGATGGCGAA 
GAGGAGGATG GTTCACGCAT GCACCGGCGA TCCATCGCCC AGAAGTCACA GCGTCGCATG 
GTTAGGCGCT CGAGCAGCAC ATCACCCAAA ACCCAGACGG CTGTGGTTAC CAAGCAGCAA 
AGCCAGTCTA TGGAGAACCT TACGCCGGAA CGTAGCGTGG CCGAGGAGCC AAAAAATATG 
CGCTTCCCAG GCAGCAACGG CGAGAACTAC AGCGTCACCC AGAACCAGGT GCTGCACATC 
CAGAAACACA CGCCTCTGTC AGTGCGCACA CGAATTCACG ATCTCGAGGC GCACCGGGCG 
GACCAGCTGC CACAGCAACC TGTCTGGACC TCGCTGACAA AACTGATTAC ACAGACCTCT 
CACTTGGGAA AAAGTGTCAG TGGCAGCAGT AGTGGAAATA TTGACTCGCG GCGGGATTCT 
AGCTGTTCGG ATGTATTCTC CTCGCAGGTG GACAGTGTTT TTGCCAAGGA TGAAGGGGAG 
AAGCGCCAGC GTCGAAAGAC GCACTCCTGG ACAGAGTCCT TGGGTCCAAG TGGCGGCATC 
GTCCTGGACC CCACGCCACA GCAACAGAAG CAGCAGTCAA ACGCCATTCT GCGTCCCCGA 
GGCACACGTC AGCGGGAGCT GCCTTCGCGG CACGCAAGTT GGGGATCAGG AGACAACCGA 
TGCTGTCTGC CGCAGAGGAC CAGTTCCGGC TCCTACTATG ACTCCAATCG CAACACTACG 
GCCATCTTCG AGGGCGTTAT CCAAGACCTC AAGCGCAGTT CCAATTGCAA TGTAATCGAA 
GGAGTCGCCG TTGCTGTGGA ACCGATAGTT GGAGTTGGCG AGGGCACCGT AAAGCGCACC 
AAGCAGAAGC TGGAAGAGAG CACCTCGCTG AAAAAGCGCT GCCAGGAAGA AAGCCAGGAG 
CTGCTACTCG AGGCTGTGGA TGCTGGGCAG AGGCGGTGCC CAAGCCTCTA TAGGAGCGCC 
TCCTCGGCGC ACAGTCCGCG GCAGCGTCAG CCACTACGCT GTAATAGTGA GGAGCTGATG 
CACACCAGCG ACATCGAGAA CAAGAACACC ACGCCAGGCG ACCATGAGGC GACGGTAGTG 
CTGCGCGTCC AGGGACAAAC GCTGGCCGAC GATCAGCGCA TCTCGGGCAA TGTGCAAATA 
CTCAAGCAAA ATTTCGAAGC CAAAGCGGGA GTGGTGGGCA CAGGAGGAGG AGGAGGCGGT 
GGCACTGCAG CGGGATCGGG CAGTAGTTCA ACAATCGCAA CGTCGGTATC GGCGGTGGCC 
GGAGCCTGCT CAGCGAATCC CGGCAAGAAG ACGACTAGCC ACCAATCACT GCCATCGTCG 
CCGGTGGCGC AGCATGCCAA CCACGTGTCC GCCGCCTCCA ACTCCAACTC CTCCGCCTCC 
AACTCGTCCG ATTCCAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163716.1
CDS	247..3387
Translation	MSMQVCSLPDDILIFSLTPRDGESEDDEGNSKGNDRSECFFAGKGTALVLALKDIPPLTQSERRLSTDSTRSSNSTQSNNSDIQLHLQSMFYLLQREDTLKMAVKLESQRSNRTRYLVIASRSCCRSGTSDRRRHRIMRHHSVKVGGSAGTKSSTSPAVPTQRQLSVEQTATEASSKCDKTADKENATAAGDNKNTSGMEESCLLGIDCNERTTIGLVVPILADTTIHLDGDGGFSVKVYEKTHIFKPVSVQAMWSALQTLHKVSKKARENNFYASGPSHDWLSSYERRIESDQSCLNEWNAMDALESRRPPSPDAIRNKPPEKEETESVIKMKLKAIMMSVDLDEVTSKYIRGRLEEILDMDLGEYKSFIDAEMLVILGQMDAPTKIFEHVYLGSEWNASNLEELQKNGVRHILNVTREIDNFFPGTFEYFNVRVYDDEKTNLLKYWDDTFRYITRAKAEGSKVLVHCKMGVSRSASVVIAYAMKAYQWEFQQALEHVKKRRSCIKPNKNFLNQLETYSGMLDAMKNKEKLQRSKSETNLKSTKDARLLPGSEPTPLIQALNQAKSKSTGEAGVTPDGEEEDGSRMHRRSIAQKSQRRMVRRSSSTSPKTQTAVVTKQQSQSMENLTPERSVAEEPKNMRFPGSNGENYSVTQNQVLHIQKHTPLSVRTRIHDLEAHRADQLPQQPVWTSLTKLITQTSHLGKSVSGSSSGNIDSRRDSSCSDVFSSQVDSVFAKDEGEKRQRRKTHSWTESLGPSGGIVLDPTPQQQKQQSNAILRPRGTRQRELPSRHASWGSGDNRCCLPQRTSSGSYYDSNRNTTAIFEGVIQDLKRSSNCNVIEGVAVAVEPIVGVGEGTVKRTKQKLEESTSLKKRCQEESQELLLEAVDAGQRRCPSLYRSASSAHSPRQRQPLRCNSEELMHTSDIENKNTTPGDHEATVVLRVQGQTLADDQRISGNVQILKQNFEAKAGVVGTGGGGGGGTAAGSGSSSTIATSVSAVAGACSANPGKKTTSHQSLPSSPVAQHANHVSAASNSNSSASNSSDSS

Target ORF information:

RefSeq Version	NM_001170245.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster slingshot (ssh), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170245.1

ORF Insert Sequence:

ATGAGCATGC AAGTTTGTTC TTTGCCAGAC GACATCCTTA TATTTAGCCT AACCCCACGG 
GATGGCGAGT CCGAGGACGA CGAGGGCAAT AGCAAAGGAA ACGATCGCAG CGAGTGCTTC 
TTTGCCGGCA AGGGAACCGC CCTGGTACTG GCTCTTAAGG ATATCCCGCC CCTGACGCAA 
AGCGAGCGTC GCCTGAGCAC CGACTCCACC CGCTCCAGTA ACAGTACGCA AAGCAACAAC 
TCGGACATCC AGCTCCACCT GCAATCAATG TTTTACCTGT TGCAGCGCGA GGACACGCTA 
AAGATGGCTG TCAAGTTGGA GTCACAGCGG TCCAACCGCA CGCGCTATCT GGTGATTGCA 
TCGCGTAGTT GTTGTCGGAG CGGCACCAGC GACCGTCGGC GCCACCGGAT CATGCGTCAC 
CACTCGGTAA AAGTTGGTGG ATCCGCGGGA ACAAAGTCGT CCACCTCCCC GGCGGTCCCC 
ACGCAGCGTC AGCTGTCCGT TGAGCAGACC GCCACAGAGG CCAGCAGCAA ATGTGATAAA 
ACAGCAGACA AGGAAAACGC AACGGCGGCC GGCGACAACA AGAATACCTC GGGCATGGAG 
GAGTCCTGCC TGCTGGGCAT AGACTGCAAC GAGCGGACCA CCATTGGCCT GGTGGTGCCC 
ATCCTGGCGG ACACCACAAT TCACCTGGAT GGCGATGGCG GATTCAGCGT CAAAGTGTAC 
GAAAAAACGC ATATATTTAA GCCGGTCTCA GTGCAGGCCA TGTGGTCCGC GCTGCAGACG 
CTGCACAAAG TGTCGAAAAA GGCTCGTGAG AACAACTTCT ATGCCAGTGG ACCCTCCCAC 
GACTGGCTAT CCAGCTACGA GAGGCGCATC GAGTCGGACC AGTCTTGTCT GAACGAGTGG 
AATGCCATGG ACGCGCTCGA GAGCCGCCGA CCACCTTCGC CTGATGCCAT ACGAAACAAA 
CCCCCCGAAA AGGAGGAAAC CGAAAGTGTC ATTAAGATGA AGCTGAAGGC CATCATGATG 
TCAGTGGACC TGGACGAGGT CACCTCCAAG TATATACGCG GACGCCTCGA GGAGATCCTG 
GACATGGATT TGGGCGAGTA CAAGTCGTTT ATCGACGCCG AAATGCTCGT TATTCTAGGC 
CAAATGGATG CCCCCACCAA GATTTTCGAG CACGTCTATC TGGGATCAGA GTGGAATGCC 
AGCAACTTGG AGGAGCTGCA GAAAAACGGC GTTCGCCACA TCCTGAACGT GACGCGGGAG 
ATCGATAACT TCTTTCCGGG CACCTTCGAG TATTTCAACG TTCGCGTGTA CGACGATGAG 
AAGACGAACC TGCTCAAGTA CTGGGACGAT ACGTTCCGCT ACATCACGCG GGCGAAAGCC 
GAGGGATCCA AGGTGCTGGT GCACTGCAAG ATGGGCGTCA GCCGATCCGC CTCGGTTGTC 
ATCGCATATG CCATGAAGGC TTACCAGTGG GAGTTCCAGC AGGCGCTAGA GCACGTGAAG 
AAGAGGCGCA GCTGTATAAA GCCCAACAAG AACTTCCTCA ATCAGCTGGA GACGTATAGT 
GGGATGTTGG ACGCCATGAA GAACAAGGAG AAGCTGCAGC GCAGCAAGAG CGAGACGAAC 
TTGAAGAGCA CAAAGGACGC GCGACTGTTG CCTGGTAGCG AACCAACGCC CCTCATTCAG 
GCTCTAAACC AGGCCAAGTC GAAGTCGACT GGTGAAGCGG GCGTAACACC CGATGGCGAA 
GAGGAGGATG GTTCACGCAT GCACCGGCGA TCCATCGCCC AGAAGTCACA GCGTCGCATG 
GTTAGGCGCT CGAGCAGCAC ATCACCCAAA ACCCAGACGG CTGTGGTTAC CAAGCAGCAA 
AGCCAGTCTA TGGAGAACCT TACGCCGGAA CGTAGCGTGG CCGAGGAGCC AAAAAATATG 
CGCTTCCCAG GCAGCAACGG CGAGAACTAC AGCGTCACCC AGAACCAGGT GCTGCACATC 
CAGAAACACA CGCCTCTGTC AGTGCGCACA CGAATTCACG ATCTCGAGGC GCACCGGGCG 
GACCAGCTGC CACAGCAACC TGTCTGGACC TCGCTGACAA AACTGATTAC ACAGACCTCT 
CACTTGGGAA AAAGTGTCAG TGGCAGCAGT AGTGGAAATA TTGACTCGCG GCGGGATTCT 
AGCTGTTCGG ATGTATTCTC CTCGCAGGTG GACAGTGTTT TTGCCAAGGA TGAAGGGGAG 
AAGCGCCAGC GTCGAAAGAC GCACTCCTGG ACAGAGTCCT TGGGTCCAAG TGGCGGCATC 
GTCCTGGACC CCACGCCACA GCAACAGAAG CAGCAGTCAA ACGCCATTCT GCGTCCCCGA 
GGCACACGTC AGCGGGAGCT GCCTTCGCGG CACGCAAGTT GGGGATCAGG AGACAACCGA 
TGCTGTCTGC CGCAGAGGAC CAGTTCCGGC TCCTACTATG ACTCCAATCG CAACACTACG 
GCCATCTTCG AGGGCGTTAT CCAAGACCTC AAGCGCAGTT CCAATTGCAA TGTAATCGAA 
GGAGTCGCCG TTGCTGTGGA ACCGATAGTT GGAGTTGGCG AGGGCACCGT AAAGCGCACC 
AAGCAGAAGC TGGAAGAGAG CACCTCGCTG AAAAAGCGCT GCCAGGAAGA AAGCCAGGAG 
CTGCTACTCG AGGCTGTGGA TGCTGGGCAG AGGCGGTGCC CAAGCCTCTA TAGGAGCGCC 
TCCTCGGCGC ACAGTCCGCG GCAGCGTCAG CCACTACGCT GTAATAGTGA GGAGCTGATG 
CACACCAGCG ACATCGAGAA CAAGAACACC ACGCCAGGCG ACCATGAGGC GACGGTAGTG 
CTGCGCGTCC AGGGACAAAC GCTGGCCGAC GATCAGCGCA TCTCGGGCAA TGTGCAAATA 
CTCAAGCAAA ATTTCGAAGC CAAAGCGGGA GTGGTGGGCA CAGGAGGAGG AGGAGGCGGT 
GGCACTGCAG CGGGATCGGG CAGTAGTTCA ACAATCGCAA CGTCGGTATC GGCGGTGGCC 
GGAGCCTGCT CAGCGAATCC CGGCAAGAAG ACGACTAGCC ACCAATCACT GCCATCGTCG 
CCGGTGGCGC AGCATGCCAA CCACGTGTCC GCCGCCTCCA ACTCCAACTC CTCCGCCTCC 
AACTCGTCCG ATTCCAGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ssh ( NM_170184.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733063.1 Drosophila melanogaster slingshot (ssh), transcript variant B, mRNA.

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CloneID	OFa08780
Clone ID Related Accession (Same CDS sequence)	NM_170184.2
Accession Version	NM_170184.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	slingshot, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_170184.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCATGC AAGTTTGTTC TTTGCCAGAC GACATCCTTA TATTTAGCCT AACCCCACGG 
GATGGCGAGT CCGAGGACGA CGAGGGCAAT AGCAAAGGAA ACGATCGCAG CGAGTGCTTC 
TTTGCCGGCA AGGGAACCGC CCTGGTACTG GCTCTTAAGG ATATCCCGCC CCTGACGCAA 
AGCGAGCGTC GCCTGAGCAC CGACTCCACC CGCTCCAGTA ACAGTACGCA AAGCAACAAC 
TCGGACATCC AGCTCCACCT GCAATCAATG TTTTACCTGT TGCAGCGCGA GGACACGCTA 
AAGATGGCTG TCAAGTTGGA GTCACAGCGG TCCAACCGCA CGCGCTATCT GGTGATTGCA 
TCGCGTAGTT GTTGTCGGAG CGGCACCAGC GACCGTCGGC GCCACCGGAT CATGCGTCAC 
CACTCGGTAA AAGTTGGTGG ATCCGCGGGA ACAAAGTCGT CCACCTCCCC GGCGGTCCCC 
ACGCAGCGTC AGCTGTCCGT TGAGCAGACC GCCACAGAGG CCAGCAGCAA ATGTGATAAA 
ACAGCAGACA AGGAAAACGC AACGGCGGCC GGCGACAACA AGAATACCTC GGGCATGGAG 
GAGTCCTGCC TGCTGGGCAT AGACTGCAAC GAGCGGACCA CCATTGGCCT GGTGGTGCCC 
ATCCTGGCGG ACACCACAAT TCACCTGGAT GGCGATGGCG GATTCAGCGT CAAAGTGTAC 
GAAAAAACGC ATATATTTAA GCCGGTCTCA GTGCAGGCCA TGTGGTCCGC GCTGCAGACG 
CTGCACAAAG TGTCGAAAAA GGCTCGTGAG AACAACTTCT ATGCCAGTGG ACCCTCCCAC 
GACTGGCTAT CCAGCTACGA GAGGCGCATC GAGTCGGACC AGTCTTGTCT GAACGAGTGG 
AATGCCATGG ACGCGCTCGA GAGCCGCCGA CCACCTTCGC CTGATGCCAT ACGAAACAAA 
CCCCCCGAAA AGGAGGAAAC CGAAAGTGTC ATTAAGATGA AGCTGAAGGC CATCATGATG 
TCAGTGGACC TGGACGAGGT CACCTCCAAG TATATACGCG GACGCCTCGA GGAGATCCTG 
GACATGGATT TGGGCGAGTA CAAGTCGTTT ATCGACGCCG AAATGCTCGT TATTCTAGGC 
CAAATGGATG CCCCCACCAA GATTTTCGAG CACGTCTATC TGGGATCAGA GTGGAATGCC 
AGCAACTTGG AGGAGCTGCA GAAAAACGGC GTTCGCCACA TCCTGAACGT GACGCGGGAG 
ATCGATAACT TCTTTCCGGG CACCTTCGAG TATTTCAACG TTCGCGTGTA CGACGATGAG 
AAGACGAACC TGCTCAAGTA CTGGGACGAT ACGTTCCGCT ACATCACGCG GGCGAAAGCC 
GAGGGATCCA AGGTGCTGGT GCACTGCAAG ATGGGCGTCA GCCGATCCGC CTCGGTTGTC 
ATCGCATATG CCATGAAGGC TTACCAGTGG GAGTTCCAGC AGGCGCTAGA GCACGTGAAG 
AAGAGGCGCA GCTGTATAAA GCCCAACAAG AACTTCCTCA ATCAGCTGGA GACGTATAGT 
GGGATGTTGG ACGCCATGAA GAACAAGGAG AAGCTGCAGC GCAGCAAGAG CGAGACGAAC 
TTGAAGAGCA CAAAGGACGC GCGACTGTTG CCTGGTAGCG AACCAACGCC CCTCATTCAG 
GCTCTAAACC AGGCCAAGTC GAAGTCGACT GGTGAAGCGG GCGTAACACC CGATGGCGAA 
GAGGAGGATG GTTCACGCAT GCACCGGCGA TCCATCGCCC AGAAGTCACA GCGTCGCATG 
GTTAGGCGCT CGAGCAGCAC ATCACCCAAA ACCCAGACGG CTGTGGTTAC CAAGCAGCAA 
AGCCAGTCTA TGGAGAACCT TACGCCGGAA CGTAGCGTGG CCGAGGAGCC AAAAAATATG 
CGCTTCCCAG GCAGCAACGG CGAGAACTAC AGCGTCACCC AGAACCAGGT GCTGCACATC 
CAGAAACACA CGCCTCTGTC AGTGCGCACA CGAATTCACG ATCTCGAGGC GCACCGGGCG 
GACCAGCTGC CACAGCAACC TGTCTGGACC TCGCTGACAA AACTGATTAC ACAGACCTCT 
CACTTGGGAA AAAGTGTCAG TGGCAGCAGT AGTGGAAATA TTGACTCGCG GCGGGATTCT 
AGCTGTTCGG ATGTATTCTC CTCGCAGGTG GACAGTGTTT TTGCCAAGGA TGAAGGGGAG 
AAGCGCCAGC GTCGAAAGAC GCACTCCTGG ACAGAGTCCT TGGGTCCAAG TGGCGGCATC 
GTCCTGGACC CCACGCCACA GCAACAGAAG CAGCAGTCAA ACGCCATTCT GCGTCCCCGA 
GGCACACGTC AGCGGGAGCT GCCTTCGCGG CACGCAAGTT GGGGATCAGG AGACAACCGA 
TGCTGTCTGC CGCAGAGGAC CAGTTCCGGC TCCTACTATG ACTCCAATCG CAACACTACG 
GCCATCTTCG AGGGCGTTAT CCAAGACCTC AAGCGCAGTT CCAATTGCAA TGTAATCGAA 
GGAGTCGCCG TTGCTGTGGA ACCGATAGTT GGAGTTGGCG AGGGCACCGT AAAGCGCACC 
AAGCAGAAGC TGGAAGAGAG CACCTCGCTG AAAAAGCGCT GCCAGGAAGA AAGCCAGGAG 
CTGCTACTCG AGGCTGTGGA TGCTGGGCAG AGGCGGTGCC CAAGCCTCTA TAGGAGCGCC 
TCCTCGGCGC ACAGTCCGCG GCAGCGTCAG CCACTACGCT GTAATAGTGA GGAGCTGATG 
CACACCAGCG ACATCGAGAA CAAGAACACC ACGCCAGGCG ACCATGAGGC GACGGTAGTG 
CTGCGCGTCC AGGGACAAAC GCTGGCCGAC GATCAGCGCA TCTCGGGCAA TGTGCAAATA 
CTCAAGCAAA ATTTCGAAGC CAAAGCGGGA GTGGTGGGCA CAGGAGGAGG AGGAGGCGGT 
GGCACTGCAG CGGGATCGGG CAGTAGTTCA ACAATCGCAA CGTCGGTATC GGCGGTGGCC 
GGAGCCTGCT CAGCGAATCC CGGCAAGAAG ACGACTAGCC ACCAATCACT GCCATCGTCG 
CCGGTGGCGC AGCATGCCAA CCACGTGTCC GCCGCCTCCA ACTCCAACTC CTCCGCCTCC 
AACTCGTCCG ATTCCAGCGT AAGCGAAAAT TGTGATCGTA ATGTAAAAGG TCTAGTGCAT 
AAATATCAAA CCACTACTTC CTGCAGTAGC CAGGCGGCCG CCACCCCCAC AGTCGCCTGT 
GCCCCACTGC CACCCGCCGG AACCGCCAGC AACATCAGCC ACTCCTGCAG CACCACTACC 
ACTACTAGCT TCAAGCAACG CCCGCTCTCC TACTACGACC GCTCGCTGTC CAACAGATCC 
ACGCATGACT ATAGCGACAG TCGACCGCCA CCCGCGCCCG TCCGCGTCAG TCACAGCAGC 
AACGTTCAGC ACCAGCAACA GCAGCAGCAA CAACTGCAAC CGGAGCAGCA TCAGCAGCAG 
GTGCAGATCC GGCGGTTGGC GCAGCACGGG AAGACGCATC CGCTGAGCAG GCTAAGTGCT 
CCGAAAACAG GCTTTAACAC CGCCGCCTAC AACACCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733063.1
CDS	247..3828
Translation	MSMQVCSLPDDILIFSLTPRDGESEDDEGNSKGNDRSECFFAGKGTALVLALKDIPPLTQSERRLSTDSTRSSNSTQSNNSDIQLHLQSMFYLLQREDTLKMAVKLESQRSNRTRYLVIASRSCCRSGTSDRRRHRIMRHHSVKVGGSAGTKSSTSPAVPTQRQLSVEQTATEASSKCDKTADKENATAAGDNKNTSGMEESCLLGIDCNERTTIGLVVPILADTTIHLDGDGGFSVKVYEKTHIFKPVSVQAMWSALQTLHKVSKKARENNFYASGPSHDWLSSYERRIESDQSCLNEWNAMDALESRRPPSPDAIRNKPPEKEETESVIKMKLKAIMMSVDLDEVTSKYIRGRLEEILDMDLGEYKSFIDAEMLVILGQMDAPTKIFEHVYLGSEWNASNLEELQKNGVRHILNVTREIDNFFPGTFEYFNVRVYDDEKTNLLKYWDDTFRYITRAKAEGSKVLVHCKMGVSRSASVVIAYAMKAYQWEFQQALEHVKKRRSCIKPNKNFLNQLETYSGMLDAMKNKEKLQRSKSETNLKSTKDARLLPGSEPTPLIQALNQAKSKSTGEAGVTPDGEEEDGSRMHRRSIAQKSQRRMVRRSSSTSPKTQTAVVTKQQSQSMENLTPERSVAEEPKNMRFPGSNGENYSVTQNQVLHIQKHTPLSVRTRIHDLEAHRADQLPQQPVWTSLTKLITQTSHLGKSVSGSSSGNIDSRRDSSCSDVFSSQVDSVFAKDEGEKRQRRKTHSWTESLGPSGGIVLDPTPQQQKQQSNAILRPRGTRQRELPSRHASWGSGDNRCCLPQRTSSGSYYDSNRNTTAIFEGVIQDLKRSSNCNVIEGVAVAVEPIVGVGEGTVKRTKQKLEESTSLKKRCQEESQELLLEAVDAGQRRCPSLYRSASSAHSPRQRQPLRCNSEELMHTSDIENKNTTPGDHEATVVLRVQGQTLADDQRISGNVQILKQNFEAKAGVVGTGGGGGGGTAAGSGSSSTIATSVSAVAGACSANPGKKTTSHQSLPSSPVAQHANHVSAASNSNSSASNSSDSSVSENCDRNVKGLVHKYQTTTSCSSQAAATPTVACAPLPPAGTASNISHSCSTTTTTSFKQRPLSYYDRSLSNRSTHDYSDSRPPPAPVRVSHSSNVQHQQQQQQQLQPEQHQQQVQIRRLAQHGKTHPLSRLSAPKTGFNTAAYNTM

Target ORF information:

RefSeq Version	NM_170184.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster slingshot (ssh), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170184.2

ORF Insert Sequence:

ATGAGCATGC AAGTTTGTTC TTTGCCAGAC GACATCCTTA TATTTAGCCT AACCCCACGG 
GATGGCGAGT CCGAGGACGA CGAGGGCAAT AGCAAAGGAA ACGATCGCAG CGAGTGCTTC 
TTTGCCGGCA AGGGAACCGC CCTGGTACTG GCTCTTAAGG ATATCCCGCC CCTGACGCAA 
AGCGAGCGTC GCCTGAGCAC CGACTCCACC CGCTCCAGTA ACAGTACGCA AAGCAACAAC 
TCGGACATCC AGCTCCACCT GCAATCAATG TTTTACCTGT TGCAGCGCGA GGACACGCTA 
AAGATGGCTG TCAAGTTGGA GTCACAGCGG TCCAACCGCA CGCGCTATCT GGTGATTGCA 
TCGCGTAGTT GTTGTCGGAG CGGCACCAGC GACCGTCGGC GCCACCGGAT CATGCGTCAC 
CACTCGGTAA AAGTTGGTGG ATCCGCGGGA ACAAAGTCGT CCACCTCCCC GGCGGTCCCC 
ACGCAGCGTC AGCTGTCCGT TGAGCAGACC GCCACAGAGG CCAGCAGCAA ATGTGATAAA 
ACAGCAGACA AGGAAAACGC AACGGCGGCC GGCGACAACA AGAATACCTC GGGCATGGAG 
GAGTCCTGCC TGCTGGGCAT AGACTGCAAC GAGCGGACCA CCATTGGCCT GGTGGTGCCC 
ATCCTGGCGG ACACCACAAT TCACCTGGAT GGCGATGGCG GATTCAGCGT CAAAGTGTAC 
GAAAAAACGC ATATATTTAA GCCGGTCTCA GTGCAGGCCA TGTGGTCCGC GCTGCAGACG 
CTGCACAAAG TGTCGAAAAA GGCTCGTGAG AACAACTTCT ATGCCAGTGG ACCCTCCCAC 
GACTGGCTAT CCAGCTACGA GAGGCGCATC GAGTCGGACC AGTCTTGTCT GAACGAGTGG 
AATGCCATGG ACGCGCTCGA GAGCCGCCGA CCACCTTCGC CTGATGCCAT ACGAAACAAA 
CCCCCCGAAA AGGAGGAAAC CGAAAGTGTC ATTAAGATGA AGCTGAAGGC CATCATGATG 
TCAGTGGACC TGGACGAGGT CACCTCCAAG TATATACGCG GACGCCTCGA GGAGATCCTG 
GACATGGATT TGGGCGAGTA CAAGTCGTTT ATCGACGCCG AAATGCTCGT TATTCTAGGC 
CAAATGGATG CCCCCACCAA GATTTTCGAG CACGTCTATC TGGGATCAGA GTGGAATGCC 
AGCAACTTGG AGGAGCTGCA GAAAAACGGC GTTCGCCACA TCCTGAACGT GACGCGGGAG 
ATCGATAACT TCTTTCCGGG CACCTTCGAG TATTTCAACG TTCGCGTGTA CGACGATGAG 
AAGACGAACC TGCTCAAGTA CTGGGACGAT ACGTTCCGCT ACATCACGCG GGCGAAAGCC 
GAGGGATCCA AGGTGCTGGT GCACTGCAAG ATGGGCGTCA GCCGATCCGC CTCGGTTGTC 
ATCGCATATG CCATGAAGGC TTACCAGTGG GAGTTCCAGC AGGCGCTAGA GCACGTGAAG 
AAGAGGCGCA GCTGTATAAA GCCCAACAAG AACTTCCTCA ATCAGCTGGA GACGTATAGT 
GGGATGTTGG ACGCCATGAA GAACAAGGAG AAGCTGCAGC GCAGCAAGAG CGAGACGAAC 
TTGAAGAGCA CAAAGGACGC GCGACTGTTG CCTGGTAGCG AACCAACGCC CCTCATTCAG 
GCTCTAAACC AGGCCAAGTC GAAGTCGACT GGTGAAGCGG GCGTAACACC CGATGGCGAA 
GAGGAGGATG GTTCACGCAT GCACCGGCGA TCCATCGCCC AGAAGTCACA GCGTCGCATG 
GTTAGGCGCT CGAGCAGCAC ATCACCCAAA ACCCAGACGG CTGTGGTTAC CAAGCAGCAA 
AGCCAGTCTA TGGAGAACCT TACGCCGGAA CGTAGCGTGG CCGAGGAGCC AAAAAATATG 
CGCTTCCCAG GCAGCAACGG CGAGAACTAC AGCGTCACCC AGAACCAGGT GCTGCACATC 
CAGAAACACA CGCCTCTGTC AGTGCGCACA CGAATTCACG ATCTCGAGGC GCACCGGGCG 
GACCAGCTGC CACAGCAACC TGTCTGGACC TCGCTGACAA AACTGATTAC ACAGACCTCT 
CACTTGGGAA AAAGTGTCAG TGGCAGCAGT AGTGGAAATA TTGACTCGCG GCGGGATTCT 
AGCTGTTCGG ATGTATTCTC CTCGCAGGTG GACAGTGTTT TTGCCAAGGA TGAAGGGGAG 
AAGCGCCAGC GTCGAAAGAC GCACTCCTGG ACAGAGTCCT TGGGTCCAAG TGGCGGCATC 
GTCCTGGACC CCACGCCACA GCAACAGAAG CAGCAGTCAA ACGCCATTCT GCGTCCCCGA 
GGCACACGTC AGCGGGAGCT GCCTTCGCGG CACGCAAGTT GGGGATCAGG AGACAACCGA 
TGCTGTCTGC CGCAGAGGAC CAGTTCCGGC TCCTACTATG ACTCCAATCG CAACACTACG 
GCCATCTTCG AGGGCGTTAT CCAAGACCTC AAGCGCAGTT CCAATTGCAA TGTAATCGAA 
GGAGTCGCCG TTGCTGTGGA ACCGATAGTT GGAGTTGGCG AGGGCACCGT AAAGCGCACC 
AAGCAGAAGC TGGAAGAGAG CACCTCGCTG AAAAAGCGCT GCCAGGAAGA AAGCCAGGAG 
CTGCTACTCG AGGCTGTGGA TGCTGGGCAG AGGCGGTGCC CAAGCCTCTA TAGGAGCGCC 
TCCTCGGCGC ACAGTCCGCG GCAGCGTCAG CCACTACGCT GTAATAGTGA GGAGCTGATG 
CACACCAGCG ACATCGAGAA CAAGAACACC ACGCCAGGCG ACCATGAGGC GACGGTAGTG 
CTGCGCGTCC AGGGACAAAC GCTGGCCGAC GATCAGCGCA TCTCGGGCAA TGTGCAAATA 
CTCAAGCAAA ATTTCGAAGC CAAAGCGGGA GTGGTGGGCA CAGGAGGAGG AGGAGGCGGT 
GGCACTGCAG CGGGATCGGG CAGTAGTTCA ACAATCGCAA CGTCGGTATC GGCGGTGGCC 
GGAGCCTGCT CAGCGAATCC CGGCAAGAAG ACGACTAGCC ACCAATCACT GCCATCGTCG 
CCGGTGGCGC AGCATGCCAA CCACGTGTCC GCCGCCTCCA ACTCCAACTC CTCCGCCTCC 
AACTCGTCCG ATTCCAGCGT AAGCGAAAAT TGTGATCGTA ATGTAAAAGG TCTAGTGCAT 
AAATATCAAA CCACTACTTC CTGCAGTAGC CAGGCGGCCG CCACCCCCAC AGTCGCCTGT 
GCCCCACTGC CACCCGCCGG AACCGCCAGC AACATCAGCC ACTCCTGCAG CACCACTACC 
ACTACTAGCT TCAAGCAACG CCCGCTCTCC TACTACGACC GCTCGCTGTC CAACAGATCC 
ACGCATGACT ATAGCGACAG TCGACCGCCA CCCGCGCCCG TCCGCGTCAG TCACAGCAGC 
AACGTTCAGC ACCAGCAACA GCAGCAGCAA CAACTGCAAC CGGAGCAGCA TCAGCAGCAG 
GTGCAGATCC GGCGGTTGGC GCAGCACGGG AAGACGCATC CGCTGAGCAG GCTAAGTGCT 
CCGAAAACAG GCTTTAACAC CGCCGCCTAC AACACCATGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ssh ( NM_079768.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524492.2 Drosophila melanogaster slingshot (ssh), transcript variant A, mRNA.

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CloneID	OFa08819
Clone ID Related Accession (Same CDS sequence)	NM_079768.4
Accession Version	NM_079768.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	slingshot, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079768.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGCTGG TGACGGTGCA GCGCTCTCCC AGTGTGGCGG GCTCCTGCTC GAATTCGGAT 
GGCGAGTCCG AGGACGACGA GGGCAATAGC AAAGGAAACG ATCGCAGCGA GTGCTTCTTT 
GCCGGCAAGG GAACCGCCCT GGTACTGGCT CTTAAGGATA TCCCGCCCCT GACGCAAAGC 
GAGCGTCGCC TGAGCACCGA CTCCACCCGC TCCAGTAACA GTACGCAAAG CAACAACTCG 
GACATCCAGC TCCACCTGCA ATCAATGTTT TACCTGTTGC AGCGCGAGGA CACGCTAAAG 
ATGGCTGTCA AGTTGGAGTC ACAGCGGTCC AACCGCACGC GCTATCTGGT GATTGCATCG 
CGTAGTTGTT GTCGGAGCGG CACCAGCGAC CGTCGGCGCC ACCGGATCAT GCGTCACCAC 
TCGGTAAAAG TTGGTGGATC CGCGGGAACA AAGTCGTCCA CCTCCCCGGC GGTCCCCACG 
CAGCGTCAGC TGTCCGTTGA GCAGACCGCC ACAGAGGCCA GCAGCAAATG TGATAAAACA 
GCAGACAAGG AAAACGCAAC GGCGGCCGGC GACAACAAGA ATACCTCGGG CATGGAGGAG 
TCCTGCCTGC TGGGCATAGA CTGCAACGAG CGGACCACCA TTGGCCTGGT GGTGCCCATC 
CTGGCGGACA CCACAATTCA CCTGGATGGC GATGGCGGAT TCAGCGTCAA AGTGTACGAA 
AAAACGCATA TATTTAAGCC GGTCTCAGTG CAGGCCATGT GGTCCGCGCT GCAGACGCTG 
CACAAAGTGT CGAAAAAGGC TCGTGAGAAC AACTTCTATG CCAGTGGACC CTCCCACGAC 
TGGCTATCCA GCTACGAGAG GCGCATCGAG TCGGACCAGT CTTGTCTGAA CGAGTGGAAT 
GCCATGGACG CGCTCGAGAG CCGCCGACCA CCTTCGCCTG ATGCCATACG AAACAAACCC 
CCCGAAAAGG AGGAAACCGA AAGTGTCATT AAGATGAAGC TGAAGGCCAT CATGATGTCA 
GTGGACCTGG ACGAGGTCAC CTCCAAGTAT ATACGCGGAC GCCTCGAGGA GATCCTGGAC 
ATGGATTTGG GCGAGTACAA GTCGTTTATC GACGCCGAAA TGCTCGTTAT TCTAGGCCAA 
ATGGATGCCC CCACCAAGAT TTTCGAGCAC GTCTATCTGG GATCAGAGTG GAATGCCAGC 
AACTTGGAGG AGCTGCAGAA AAACGGCGTT CGCCACATCC TGAACGTGAC GCGGGAGATC 
GATAACTTCT TTCCGGGCAC CTTCGAGTAT TTCAACGTTC GCGTGTACGA CGATGAGAAG 
ACGAACCTGC TCAAGTACTG GGACGATACG TTCCGCTACA TCACGCGGGC GAAAGCCGAG 
GGATCCAAGG TGCTGGTGCA CTGCAAGATG GGCGTCAGCC GATCCGCCTC GGTTGTCATC 
GCATATGCCA TGAAGGCTTA CCAGTGGGAG TTCCAGCAGG CGCTAGAGCA CGTGAAGAAG 
AGGCGCAGCT GTATAAAGCC CAACAAGAAC TTCCTCAATC AGCTGGAGAC GTATAGTGGG 
ATGTTGGACG CCATGAAGAA CAAGGAGAAG CTGCAGCGCA GCAAGAGCGA GACGAACTTG 
AAGAGCACAA AGGACGCGCG ACTGTTGCCT GGTAGCGAAC CAACGCCCCT CATTCAGGCT 
CTAAACCAGG CCAAGTCGAA GTCGACTGGT GAAGCGGGCG TAACACCCGA TGGCGAAGAG 
GAGGATGGTT CACGCATGCA CCGGCGATCC ATCGCCCAGA AGTCACAGCG TCGCATGGTT 
AGGCGCTCGA GCAGCACATC ACCCAAAACC CAGACGGCTG TGGTTACCAA GCAGCAAAGC 
CAGTCTATGG AGAACCTTAC GCCGGAACGT AGCGTGGCCG AGGAGCCAAA AAATATGCGC 
TTCCCAGGCA GCAACGGCGA GAACTACAGC GTCACCCAGA ACCAGGTGCT GCACATCCAG 
AAACACACGC CTCTGTCAGT GCGCACACGA ATTCACGATC TCGAGGCGCA CCGGGCGGAC 
CAGCTGCCAC AGCAACCTGT CTGGACCTCG CTGACAAAAC TGATTACACA GACCTCTCAC 
TTGGGAAAAA GTGTCAGTGG CAGCAGTAGT GGAAATATTG ACTCGCGGCG GGATTCTAGC 
TGTTCGGATG TATTCTCCTC GCAGGTGGAC AGTGTTTTTG CCAAGGATGA AGGGGAGAAG 
CGCCAGCGTC GAAAGACGCA CTCCTGGACA GAGTCCTTGG GTCCAAGTGG CGGCATCGTC 
CTGGACCCCA CGCCACAGCA ACAGAAGCAG CAGTCAAACG CCATTCTGCG TCCCCGAGGC 
ACACGTCAGC GGGAGCTGCC TTCGCGGCAC GCAAGTTGGG GATCAGGAGA CAACCGATGC 
TGTCTGCCGC AGAGGACCAG TTCCGGCTCC TACTATGACT CCAATCGCAA CACTACGGCC 
ATCTTCGAGG GCGTTATCCA AGACCTCAAG CGCAGTTCCA ATTGCAATGT AATCGAAGGA 
GTCGCCGTTG CTGTGGAACC GATAGTTGGA GTTGGCGAGG GCACCGTAAA GCGCACCAAG 
CAGAAGCTGG AAGAGAGCAC CTCGCTGAAA AAGCGCTGCC AGGAAGAAAG CCAGGAGCTG 
CTACTCGAGG CTGTGGATGC TGGGCAGAGG CGGTGCCCAA GCCTCTATAG GAGCGCCTCC 
TCGGCGCACA GTCCGCGGCA GCGTCAGCCA CTACGCTGTA ATAGTGAGGA GCTGATGCAC 
ACCAGCGACA TCGAGAACAA GAACACCACG CCAGGCGACC ATGAGGCGAC GGTAGTGCTG 
CGCGTCCAGG GACAAACGCT GGCCGACGAT CAGCGCATCT CGGGCAATGT GCAAATACTC 
AAGCAAAATT TCGAAGCCAA AGCGGGAGTG GTGGGCACAG GAGGAGGAGG AGGCGGTGGC 
ACTGCAGCGG GATCGGGCAG TAGTTCAACA ATCGCAACGT CGGTATCGGC GGTGGCCGGA 
GCCTGCTCAG CGAATCCCGG CAAGAAGACG ACTAGCCACC AATCACTGCC ATCGTCGCCG 
GTGGCGCAGC ATGCCAACCA CGTGTCCGCC GCCTCCAACT CCAACTCCTC CGCCTCCAAC 
TCGTCCGATT CCAGCGTAAG CGAAAATTGT GATCGTAATG TAAAAGGTCT AGTGCATAAA 
TATCAAACCA CTACTTCCTG CAGTAGCCAG GCGGCCGCCA CCCCCACAGT CGCCTGTGCC 
CCACTGCCAC CCGCCGGAAC CGCCAGCAAC ATCAGCCACT CCTGCAGCAC CACTACCACT 
ACTAGCTTCA AGCAACGCCC GCTCTCCTAC TACGACCGCT CGCTGTCCAA CAGATCCACG 
CATGACTATA GCGACAGTCG ACCGCCACCC GCGCCCGTCC GCGTCAGTCA CAGCAGCAAC 
GTTCAGCACC AGCAACAGCA GCAGCAACAA CTGCAACCGG AGCAGCATCA GCAGCAGGTG 
CAGATCCGGC GGTTGGCGCA GCACGGGAAG ACGCATCCGC TGAGCAGGCT AAGTGCTCCG 
AAAACAGGCT TTAACACCGC CGCCTACAAC ACCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524492.2
CDS	483..4061
Translation	MALVTVQRSPSVAGSCSNSDGESEDDEGNSKGNDRSECFFAGKGTALVLALKDIPPLTQSERRLSTDSTRSSNSTQSNNSDIQLHLQSMFYLLQREDTLKMAVKLESQRSNRTRYLVIASRSCCRSGTSDRRRHRIMRHHSVKVGGSAGTKSSTSPAVPTQRQLSVEQTATEASSKCDKTADKENATAAGDNKNTSGMEESCLLGIDCNERTTIGLVVPILADTTIHLDGDGGFSVKVYEKTHIFKPVSVQAMWSALQTLHKVSKKARENNFYASGPSHDWLSSYERRIESDQSCLNEWNAMDALESRRPPSPDAIRNKPPEKEETESVIKMKLKAIMMSVDLDEVTSKYIRGRLEEILDMDLGEYKSFIDAEMLVILGQMDAPTKIFEHVYLGSEWNASNLEELQKNGVRHILNVTREIDNFFPGTFEYFNVRVYDDEKTNLLKYWDDTFRYITRAKAEGSKVLVHCKMGVSRSASVVIAYAMKAYQWEFQQALEHVKKRRSCIKPNKNFLNQLETYSGMLDAMKNKEKLQRSKSETNLKSTKDARLLPGSEPTPLIQALNQAKSKSTGEAGVTPDGEEEDGSRMHRRSIAQKSQRRMVRRSSSTSPKTQTAVVTKQQSQSMENLTPERSVAEEPKNMRFPGSNGENYSVTQNQVLHIQKHTPLSVRTRIHDLEAHRADQLPQQPVWTSLTKLITQTSHLGKSVSGSSSGNIDSRRDSSCSDVFSSQVDSVFAKDEGEKRQRRKTHSWTESLGPSGGIVLDPTPQQQKQQSNAILRPRGTRQRELPSRHASWGSGDNRCCLPQRTSSGSYYDSNRNTTAIFEGVIQDLKRSSNCNVIEGVAVAVEPIVGVGEGTVKRTKQKLEESTSLKKRCQEESQELLLEAVDAGQRRCPSLYRSASSAHSPRQRQPLRCNSEELMHTSDIENKNTTPGDHEATVVLRVQGQTLADDQRISGNVQILKQNFEAKAGVVGTGGGGGGGTAAGSGSSSTIATSVSAVAGACSANPGKKTTSHQSLPSSPVAQHANHVSAASNSNSSASNSSDSSVSENCDRNVKGLVHKYQTTTSCSSQAAATPTVACAPLPPAGTASNISHSCSTTTTTSFKQRPLSYYDRSLSNRSTHDYSDSRPPPAPVRVSHSSNVQHQQQQQQQLQPEQHQQQVQIRRLAQHGKTHPLSRLSAPKTGFNTAAYNTM

Target ORF information:

RefSeq Version	NM_079768.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster slingshot (ssh), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079768.4

ORF Insert Sequence:

ATGGCGCTGG TGACGGTGCA GCGCTCTCCC AGTGTGGCGG GCTCCTGCTC GAATTCGGAT 
GGCGAGTCCG AGGACGACGA GGGCAATAGC AAAGGAAACG ATCGCAGCGA GTGCTTCTTT 
GCCGGCAAGG GAACCGCCCT GGTACTGGCT CTTAAGGATA TCCCGCCCCT GACGCAAAGC 
GAGCGTCGCC TGAGCACCGA CTCCACCCGC TCCAGTAACA GTACGCAAAG CAACAACTCG 
GACATCCAGC TCCACCTGCA ATCAATGTTT TACCTGTTGC AGCGCGAGGA CACGCTAAAG 
ATGGCTGTCA AGTTGGAGTC ACAGCGGTCC AACCGCACGC GCTATCTGGT GATTGCATCG 
CGTAGTTGTT GTCGGAGCGG CACCAGCGAC CGTCGGCGCC ACCGGATCAT GCGTCACCAC 
TCGGTAAAAG TTGGTGGATC CGCGGGAACA AAGTCGTCCA CCTCCCCGGC GGTCCCCACG 
CAGCGTCAGC TGTCCGTTGA GCAGACCGCC ACAGAGGCCA GCAGCAAATG TGATAAAACA 
GCAGACAAGG AAAACGCAAC GGCGGCCGGC GACAACAAGA ATACCTCGGG CATGGAGGAG 
TCCTGCCTGC TGGGCATAGA CTGCAACGAG CGGACCACCA TTGGCCTGGT GGTGCCCATC 
CTGGCGGACA CCACAATTCA CCTGGATGGC GATGGCGGAT TCAGCGTCAA AGTGTACGAA 
AAAACGCATA TATTTAAGCC GGTCTCAGTG CAGGCCATGT GGTCCGCGCT GCAGACGCTG 
CACAAAGTGT CGAAAAAGGC TCGTGAGAAC AACTTCTATG CCAGTGGACC CTCCCACGAC 
TGGCTATCCA GCTACGAGAG GCGCATCGAG TCGGACCAGT CTTGTCTGAA CGAGTGGAAT 
GCCATGGACG CGCTCGAGAG CCGCCGACCA CCTTCGCCTG ATGCCATACG AAACAAACCC 
CCCGAAAAGG AGGAAACCGA AAGTGTCATT AAGATGAAGC TGAAGGCCAT CATGATGTCA 
GTGGACCTGG ACGAGGTCAC CTCCAAGTAT ATACGCGGAC GCCTCGAGGA GATCCTGGAC 
ATGGATTTGG GCGAGTACAA GTCGTTTATC GACGCCGAAA TGCTCGTTAT TCTAGGCCAA 
ATGGATGCCC CCACCAAGAT TTTCGAGCAC GTCTATCTGG GATCAGAGTG GAATGCCAGC 
AACTTGGAGG AGCTGCAGAA AAACGGCGTT CGCCACATCC TGAACGTGAC GCGGGAGATC 
GATAACTTCT TTCCGGGCAC CTTCGAGTAT TTCAACGTTC GCGTGTACGA CGATGAGAAG 
ACGAACCTGC TCAAGTACTG GGACGATACG TTCCGCTACA TCACGCGGGC GAAAGCCGAG 
GGATCCAAGG TGCTGGTGCA CTGCAAGATG GGCGTCAGCC GATCCGCCTC GGTTGTCATC 
GCATATGCCA TGAAGGCTTA CCAGTGGGAG TTCCAGCAGG CGCTAGAGCA CGTGAAGAAG 
AGGCGCAGCT GTATAAAGCC CAACAAGAAC TTCCTCAATC AGCTGGAGAC GTATAGTGGG 
ATGTTGGACG CCATGAAGAA CAAGGAGAAG CTGCAGCGCA GCAAGAGCGA GACGAACTTG 
AAGAGCACAA AGGACGCGCG ACTGTTGCCT GGTAGCGAAC CAACGCCCCT CATTCAGGCT 
CTAAACCAGG CCAAGTCGAA GTCGACTGGT GAAGCGGGCG TAACACCCGA TGGCGAAGAG 
GAGGATGGTT CACGCATGCA CCGGCGATCC ATCGCCCAGA AGTCACAGCG TCGCATGGTT 
AGGCGCTCGA GCAGCACATC ACCCAAAACC CAGACGGCTG TGGTTACCAA GCAGCAAAGC 
CAGTCTATGG AGAACCTTAC GCCGGAACGT AGCGTGGCCG AGGAGCCAAA AAATATGCGC 
TTCCCAGGCA GCAACGGCGA GAACTACAGC GTCACCCAGA ACCAGGTGCT GCACATCCAG 
AAACACACGC CTCTGTCAGT GCGCACACGA ATTCACGATC TCGAGGCGCA CCGGGCGGAC 
CAGCTGCCAC AGCAACCTGT CTGGACCTCG CTGACAAAAC TGATTACACA GACCTCTCAC 
TTGGGAAAAA GTGTCAGTGG CAGCAGTAGT GGAAATATTG ACTCGCGGCG GGATTCTAGC 
TGTTCGGATG TATTCTCCTC GCAGGTGGAC AGTGTTTTTG CCAAGGATGA AGGGGAGAAG 
CGCCAGCGTC GAAAGACGCA CTCCTGGACA GAGTCCTTGG GTCCAAGTGG CGGCATCGTC 
CTGGACCCCA CGCCACAGCA ACAGAAGCAG CAGTCAAACG CCATTCTGCG TCCCCGAGGC 
ACACGTCAGC GGGAGCTGCC TTCGCGGCAC GCAAGTTGGG GATCAGGAGA CAACCGATGC 
TGTCTGCCGC AGAGGACCAG TTCCGGCTCC TACTATGACT CCAATCGCAA CACTACGGCC 
ATCTTCGAGG GCGTTATCCA AGACCTCAAG CGCAGTTCCA ATTGCAATGT AATCGAAGGA 
GTCGCCGTTG CTGTGGAACC GATAGTTGGA GTTGGCGAGG GCACCGTAAA GCGCACCAAG 
CAGAAGCTGG AAGAGAGCAC CTCGCTGAAA AAGCGCTGCC AGGAAGAAAG CCAGGAGCTG 
CTACTCGAGG CTGTGGATGC TGGGCAGAGG CGGTGCCCAA GCCTCTATAG GAGCGCCTCC 
TCGGCGCACA GTCCGCGGCA GCGTCAGCCA CTACGCTGTA ATAGTGAGGA GCTGATGCAC 
ACCAGCGACA TCGAGAACAA GAACACCACG CCAGGCGACC ATGAGGCGAC GGTAGTGCTG 
CGCGTCCAGG GACAAACGCT GGCCGACGAT CAGCGCATCT CGGGCAATGT GCAAATACTC 
AAGCAAAATT TCGAAGCCAA AGCGGGAGTG GTGGGCACAG GAGGAGGAGG AGGCGGTGGC 
ACTGCAGCGG GATCGGGCAG TAGTTCAACA ATCGCAACGT CGGTATCGGC GGTGGCCGGA 
GCCTGCTCAG CGAATCCCGG CAAGAAGACG ACTAGCCACC AATCACTGCC ATCGTCGCCG 
GTGGCGCAGC ATGCCAACCA CGTGTCCGCC GCCTCCAACT CCAACTCCTC CGCCTCCAAC 
TCGTCCGATT CCAGCGTAAG CGAAAATTGT GATCGTAATG TAAAAGGTCT AGTGCATAAA 
TATCAAACCA CTACTTCCTG CAGTAGCCAG GCGGCCGCCA CCCCCACAGT CGCCTGTGCC 
CCACTGCCAC CCGCCGGAAC CGCCAGCAAC ATCAGCCACT CCTGCAGCAC CACTACCACT 
ACTAGCTTCA AGCAACGCCC GCTCTCCTAC TACGACCGCT CGCTGTCCAA CAGATCCACG 
CATGACTATA GCGACAGTCG ACCGCCACCC GCGCCCGTCC GCGTCAGTCA CAGCAGCAAC 
GTTCAGCACC AGCAACAGCA GCAGCAACAA CTGCAACCGG AGCAGCATCA GCAGCAGGTG 
CAGATCCGGC GGTTGGCGCA GCACGGGAAG ACGCATCCGC TGAGCAGGCT AAGTGCTCCG 
AAAACAGGCT TTAACACCGC CGCCTACAAC ACCATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ssh cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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