puf cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following puf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the puf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa05977 NM_170158.3
Latest version!
Drosophila melanogaster puffyeye (puf), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OFa05978 NM_143018.4
Latest version!
Drosophila melanogaster puffyeye (puf), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OFa05979 NM_206564.2
Latest version!
Drosophila melanogaster puffyeye (puf), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OFa07738 NM_001275994.2
Latest version!
Drosophila melanogaster puffyeye (puf), transcript variant H, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OFa07739 NM_001275993.2
Latest version!
Drosophila melanogaster puffyeye (puf), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OFa22151 NM_001300579.1
Latest version!
Drosophila melanogaster puffyeye (puf), transcript variant I, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa05977
    Clone ID Related Accession (Same CDS sequence) NM_170158.3
    Accession Version NM_170158.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11739bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product puffyeye, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_170158.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    ATGTGCGAGG TGTGCGCCGA CTTCCAGAAT CTCCTGGAGC TCTATGAGGT GCGCGTGGCC 
    AGCTCGGATC TGAAGTTCCA GCTGCTGCTG AAGAGCGAGA TCGAGACCAC CTTCAACTAC
    ATCCAGTCGT GGCCGCAGCG TCAGTGCATG TGCCTGTACC GGGACACCAA GAACTATGAC
    CGTTTCAACC TGGTAGTGCA GTCGCTCATC TGTCTGACGG TGCAGCATCT GAAGCACATC
    GACCACCTCA TCGACAACTA CAAGCGGCTA ACGGCCAGTG CTGCCCAGGT GGTGGCCCAG
    GCCCAGCAGC AGCGTGAACA GCAGCGGGAC GAAGCGTCAG CCCAGGCAGA GGCAAAGGAA
    TCATCCGCGC CAGCGGAAGA ACCTAAGAAG GAGGAGCCAT CTGGGTCCGC CGGTGAGGAG
    GCTCAGGGCT CCGGCGACGG GCCAGCCAAA AAGCCGCCAG TTGGCCCCTG CACTCCCCCT
    CCCCCGCAAA CGGCCAATCC TCAGCACAAA TCCCACCTGC AGTACACAGA GGAGCCCTGG
    ATCCTGCCCG AGGTGGAGAA ACTGTTGGTG CTCGTTTCAA AGGTGTTCCT GCTCAACTTC
    CCGCTGTACA TCGCCCACAA ACATGGCATG CACTCTCGGC TGGACGACCT GCAGGCTGAG
    GAGGCCCACC ACCTGGCCCT CATCTGCGAT CTTCACGACA ACGACCTGCC CATCTATCTG
    CTGCGGAATG TGAGCCTGTT CTGCAACTCG GGCGGCTTTG GCGCCATGTC GCTGTGCTTC
    GAACATCCCG ATCTGCCAGT GTCCACCGCT CACTCGATGA CCGCTGCCGT CTCAAATGTG
    AAACTTTGGC TGAACTATCA CTGTAATACT CAGCTCTTCG TGCCGCTGCG AAGTCGGATA
    CTGCAGTACA TGTGCAAGCT GTCGGATCAG AGCCTCCGAT CTGCCGCAAC CCGCGCGATG
    GCCGATTTTG TGTGGTCCTC TATGCGAGAC CCACTGGATG TTGCTGTCAA CTTCGACACC
    GAAGGATTGG CCCTGGCCTT TAAGTACTTC ACATCGACCA CGCTAACCAT GCGACTGGCA
    GGCATGGCCC AGATCAACGC ACACATTAAT CTATTTAACG AGATCTGCAC CACCGAAACG
    GTCAACGAAG TGGAGCTGTT CGGGCAGCGC ATCGCGAACT GGCTGACTGA AAATCATATT
    GTACAGCACC TGTTTGGCCC GAATCTGCAC GTAGAAATCG TTAAACAGGC ACACGTGCTG
    CTCAACTTTT TGGCGGTAGA GAACCAGATC TCTGAAGAGG ACATCAAGCT GATCTGGCAA
    GCCACCCAGC TGAAGCACTG CTCAAAGACG ATCTTCGACA TCCTGCCTTC GCTGGTGAAG
    AATCTGACCC CGCGCCCGGC AATGCACCTT TACTCCCTCC TTTGCCGCAT GGATCCCAAG
    GAGCACACTG AGCAGAGCAT ATACATAGCC TCAGCGCTAA CAAAACAGCT GTGGACTCGT
    GATACCTCAC GCAGTCAGAT GAACCTGATG CAAGACCACC TGCTGGGGTC CAACGTCACC
    GCTTCCAGCT CTGACAGCGG CAGCATTGAG GGCAGCAACA CAGAGGACGA CCACGTGGGC
    GCCGACGACA GTAGCATTGC CAGCGGTGGC GGCGGCGGAG GTGTTGGTAA CAAGAGTCCA
    ATCGATGGAG TTACTCCATG CAAGCAGGCC CGGCACAGGC GGCACATCTG TGACCCCACC
    ACCGAGAAGG GAAAGCAAAT AAGTCCCGAG GATATGGCCA AAGTAGACTT GGTGAACAAA
    CGGATTGTGA ACATTATCGA CAACACAAGC AGTGAAGAGG AGCTGCAATC GCGGGCCGCA
    CTGGAGCTGC AACTGCATCG CAGCCGCAAA AAGACGAGCA ACAAGCGACG GCGACAAAAA
    ACCAACAAAC AGATCATTCT GCCCCATGAG CTGGTCGAGA TTTGGGACGG AGTTGAAGAT
    GTGCCCAGCA GTGACGAGGC TGATGGCGAA GCCGATGGTG ATGGCGAGGG CGAACTTTTA
    GCGGACAGCG ATGAGTGCAG CGATGGTGCT ACGAGTCAGC TGGTACCCGA CGCCGTGCTA
    AAGCATCTGC AGGGAGAGGG ACCTTTTATC AGGGCCATCG AGACCAATAT CAATGAGCTG
    CTCAGTGGTG CCGAGAACGA TGGCAGTTAC TCATCCCCGA TGAGTAACAA ATCTGAAAAG
    AACCTGGCTG ACTTCGATGA CGAGGACGTG TCGCCTTGCG AGGAGGAGCT GGCCCAGCTG
    GTCAGCTCCC GCGCCAATTG TTCGGACGTT CCACCAGCCT TTGCAGCCGC TGCCGCCGCC
    ATGATGGTGG CCCAGTCGGC GATGGCCCTT CAGAAGTCTG GAGAGTCGAA TGTGGCTGCT
    GCTGCCGCTG CTGTGGCCGC TGCTGCAGCG GCTACCGGAA CTGCACAGCA AACGCTGGTG
    GCAATGAACC GGCAGGCCAG CGTTGCTGCA GCTGCCGCCG TGGTTGCTGC GGCCAAGGCA
    AAATCGGACT CAGATGTCGA TCTGATGGAT GTGGTTTCCG GCGGAAAGCA GCACCATTCA
    CCAAAGGCGA GCCAGGGCAG CTCTACGTCC GGGAGTACAC CGGTGCAGCC CTCGTTCAAG
    CTAAATGATG TCTGCCAGCC CGGAAACACA CTGCTTTGGG ATCTGCTTCA AGACGATAAA
    ATTGGCCAAC TAGGCGAGTC TCTGGCTCTA GAGGCGGAGA AAGCTCTGGC CACTCTCCTG
    TGCTTCAGCA TGGACCGCCA ACTGCGAACG AAATTCATCG AGGGTTGTCT GTATAACGTC
    GCCAACAACC GGAGTGTTAT TGTCAGTCTG CGACTGCTGC CCAAACTGTT CGCCTCGTTC
    CAACAGTTTC GACCCTCGGA CACGCACTCT ATGACGATGT GGGCAGAGCG AAATCACCGG
    ATGATGCAGT GCTTTTTCAA TAATATACGT CACTATGCGC GTCGCCATGC CGAGGTACTG
    ATCACCCAAA ACGGAGAGCA GCAGCAGCAG CAGCTGGGCG GACAACTCTA CTCGCACAAG
    ACACAAGTTT CTGTAAGACT GCAGTTCCTA TCATCGATAT TCTCGACGGT GGGATCCCCG
    AAAAGCTTCC GCCTAACGCT GGAGCAGCTT GACGCGCTAT GGGAGTGGCT GGCCCACGAT
    CCGGAGTGTG CCGACTGCTA CTTTTCGTGG CTGCAGGCCC AGGCCAAAGG CGGTGATCAG
    CATGCTCTGG GCATTGAAGC ACTTCAGCAT TTGTATTTGA AGAAACTCCC AGAGCTGCGA
    CCCGAGGAGT TCTCTATGGT TGCGTTGGGA CTATTTCAAC AGCTCTGTAG CTTCGCCCGC
    ATCGCCATGG CCGAGTACGA CAATCATAGC GACCAGATCT CGGCGAGCGC CAGTGCCGTT
    GGCATGTACC ATCTTTGGAA GATAGCGTTG CGTGCCCAGA GCAACGATGT CTCCTTGGCG
    GCCATACAGT ACATCAACAT GTACTACATG GGTCAACAAT TGCGCCTGGA GAAGGAGTTC
    GTTTCACAGT GTATGGAAAA CCTAGTTCAA GCGGCCACGG CTTTGGAAAG CATCGATGAT
    GAGAATGCTT TGATGCGTGT CCAGCGTGGC CTGCTCTTGC TGAACACCCA TTTGGATACG
    TTCCGGCGTC GATATGCGTT TCATCTGCGC CGCTGGGCCA TCGAAGGCAA AGGGATTGGA
    TCGCACAGTA ACCTTAAAAA TGAGGGAGCG GGTCCACCAC TACGAATAGT GCTGCAACAG
    GCAGGACTTT CGGAAAAAAG CCTTTTGCAG ATGCACGCGT GTGACTTAAT CGCCGACCTC
    AAGGCTGAGG TGTCCAAGTG GTGGGAGAGC CTTCAAACTG GACTAGCTGC TCCTGTGCTC
    GGTCTGCTGC TCAGCGATGG ACCGCTACGC ATTATCACAC AGGGACAGGA ATTGACCTCG
    GATTACGATG AACGGAGTCT CGGAGATGCT GGTTTCAAGG ATAACCAGAT CGTCTACGTA
    TCGTTAGGTG GACGTGGAGC CAGGCGAAAG GAATCCAATT TGGAGCATCC GTCAATGCTG
    CCGCCCCCAC CAAAGGAGTG TTTGCCCACG GTGCTCCTGC TGCAGCCAAA GTACTTCGAG
    AAGCTATTTT GCCTGATGCA GACCCTGGGC GATATGCAAC CGCAAGCGTC GACTGTAAAT
    CCCCAGCACC ACACAAAGGC GCAATTGCTC TCGCGACGCG TATGGGATAT CCTCGCCATG
    CTGCCAACTA ATCCGCACAT TCTAGACGCC TTTAAGAGTC TGGTTACGGA CCTAAGCGAA
    CTCGAGCAGC TTGATGCTGG CGGAGAGGAG GAGCAACTGG CCACCAAGCG CAAGCAAATC
    AAGCAGAAGT TCCGAGATCT TCTCGACCCG AACAACTTGC AAAAGTTCAT GTACTCCCTC
    CATATTGTGG AGAGTCTGGC CCTAACAAGT TCCAGACGTG GTGAATCCAA CGGCAATGTG
    GCCATGGGAA ATACACCGGA ACAGGTACGG GTGAAGAAAT CCTCGATGGG AAGACGGAGA
    AATAGCAACG AGCAGCCGCC ACCGCCGCCG CCAGAAGTCA AGATGAGCAA GGAGCAACTG
    TGCGAGCTGG AGGCCCCACT GACGCCCACA CCCTCGACTG GCTTGCAGGA TGTGGAAACG
    GAGGCCAGCA GTAGCAGCGG TGGCGACAAA GAAAACCAAC CGAAACAACA CAGCAAGCGT
    CAGAAGAAAG GCGAAACTTT TGAACAGGAA AAGGAGCGCC CAGTGGGTTG CTCCACGCCG
    CCGTCTCCAA CTCCTCCGCC ACCAGCACTT TCGGTAGTGG AAAGAGGTGA CAATAAGTGG
    AGTGAGGCGT TCGTCAAATG TGGTGGCCTT CGTCATCTGT ACGAAATCTT TAGTGAGGGC
    CAACTACAAC AAAGCGCACA TCCCAAGGAG TTGGCTTTAA ATGAATGGCG ACACGATTGC
    CTGGCCAGCC TGCTGCGCAT TCTCTGGCTT CTTGGATTCG AGGAACTGCA GTCGGCGGAT
    GCCCATGTTC TAATGTCACG TCCGCACCCG TTTATGCTAC AGTTGATGGA GGTACCACAG
    TGCCTGACCC GACTCTCATC GATCCTTAAC GACGAAGTTC ACCAGCAGCA TCAGGCTTCT
    TCGGCCAATC CACTGGTATT CCCCTATCAG TTCCAGCACT TGCGTACTGG CTTTTGGGGC
    AGGGCCCAGC TCATCCAGTT CGCTATGAAC ATTCTAGTGA GTTTTGTGCA CGCCTCAGCG
    GAAGCGAGAA GGCTTCTCTG GGCCCCAACT GGTACCGATC ACTGTCGATG GCTGCAGAAG
    TTCATCCTGG AGGATCCCGA ACCGGCGGTA CGGCGTGAGA TATGCGCTGG TCTTTATCGC
    ATTTGCTTAG GAAACGCACA TAGCTATCGC CTCTTACTAG CACCACTGCT GCACAAGCTC
    ATCGCCCTGT TGCCATTGGC CGAGCAGATG AGCTCTGGCA ACCAGCACAC GCAGTTTTTG
    CTCTCGGAAG AGGGCAAGGA TCCCTACGGA CCCGCCTGCC GTGACTACTT TTGGCTGCTG
    GCCAGATTGG TGGATACTCT CTCCCCGGAA ATGGTGGCCG AGGAGCACAT CGACATTGAA
    ATGCTGTGCG AAAGCATCTC GCAGAGCATT CTCACCCGAG AGTATTATGA ACTGCGCCAT
    GGTTACCAGG ACGATGGCTT GGTGGGTCTC CTAAATCTCA TGTCCAATTT GATCAAGTAC
    GACACCACAT TCAAGTACAC ACCCAAGGCG TTATCCTTTA TTGAGCAACT GATTGGCTTC
    CTGTTTGACA TGCCCTCGCC GGCAGATCGC CAGAAGCCCA AGTGCAAGTC GGCGTCGTCT
    CGAGCATCTG CCTACGATCT GCTGGTTGAA CTTTGCCGTG GATGCGCCAC AAACTATGCA
    TATCTGCATG GACGTCTGTT GGCACAGCAC AAATCCGGAC CCAAGCAGCC GTATCCCTGG
    GACTATTGGC CGCGCGATGA AGGCAGAGCT GAGTGCGGAT ATGTGGGGCT AACTAATTTG
    GGAGCCACTT GCTATATGGC CTCTTGCGTC CAGCACTTGT ACATGATGCC ACAGGCGCGA
    GCAGCTGTTT TGCGAGTTCC ACCCAACGCC GCCCGTAAGC ATGGGCCCAC ACTTCTGGAA
    CTACAACGGA TGTTTGCCTA CCTACTGGAG TCGGAACGGA AGTCTTACAA CCCGCGAAGC
    TTCTGCCGGG TGTACCAGAT GGATCACCAG CCGCTAAATA CGGGAGAGCA AAAGGATATG
    GCAGAGTTCT TTATCGATTT GGTGTCCAAA CTGGAAGATA TGACGCCTGA CTTGAAGCAC
    CTGGTGAAAA GGCTCTTCTG TGGATCCTTG AGCAATAATG TCGTCTCCCT GGATTGCGGT
    CACGTTTCCC GCACGGCCGA GGACTTTTAC ACGGTTCGCT GCCAGGTTGC AGATATGCGC
    AATCTGCAGG AATCCCTGGA CGAAGTCACC GTTAAGGATA CCCTCGAGGG CGACAACATG
    TACACCTGCT CGCAGTGCGG CAAAAAAGTA CGAGCCGAGA AGCGGGCTTG CTTCAAGAAG
    CTTCCCCAGA TCTTGTGCTT CAACACCATG CGCTACACCT TCAATATGGT CACCATGCTC
    AAGGAGAAGG TCAACACGCA CTTTTCCTTT CCGCTACGCC TGAACATGTG TCACTATGTG
    GAGAAGACCT TGATGCCGCA GCAGTACAAA GAGGAGCGAG AAAGGCGCCA AAAGGAGAAG
    GAAGGAGCAG ATGGTAGTGG CGATGGGAAT GACAACGAAA AGGCAGAGGC CACGCTAGAT
    GACGATATTG AAGAATGCTA CGAGTACGAA CTGGTGGGCG TCACTGTCCA CACGGGCACA
    GCGGATGGCG GCCACTACTA CAGCTTTATA AAGGAGCGGA CGAAGACTAG TTACCATACG
    CACGAGCGGT GGTTTCTTTT CAACGATGCC GAAGTGAAGC CTTTCGATCC CTCACAGATA
    GCGGCGGAGT GCTTTGGCGG CGAGATGACG AGCAAAACCT ACGACTCAGT GACGGAGAAA
    TATCTGGACT TTAGTTTCGA GAAGACCAAC TCTGCCTACA TGCTGTTCTA CGAGCGCCGA
    CTGCCAGAGC ATCTGCAGCG CCGGCATTCC GAGCTGCTGG TTACACCCAC GCCCAGTCCC
    ACAGTGGAAG AGAAGTCCGA GGCGGAGGAG CCCACTAAAA TGGAGACAAG CTCTAGCGAA
    ATCAAGGCAG ATGTGGATGT GGAAGTGGAA GTTGAGGAGA AGGACAAGGA GAAGCCTGCG
    CAGACTGACA CAGAATCTAA GGAAACGCCT GCAAAAGAGG AGATCGCGGA TGACAAATCG
    AAGCAGGATG AGCCTGAGGA AAAGAAAATC GAAAAGCAAT CCAGAGAGGG CGAAGAGAAA
    TCGGAAACGG ACGAAAAGCC CACGGAGATG ACGACCGTGA GCACTGAGGA GGAAAAGCAG
    CCAACAGCCA ATTGTGATAA TCACCAGCAA AACAACAATA GCAACAGCAA AGCCAGCAAC
    GATCAGCAAC CATCCACGTC GAAGGCTGCC CAGAAGCTAC AACTATTTCG TCCATTGTTG
    AACAAGGAGC TCGAGGATTG GATTTGGCAG GACAATCGAC AGTTCCTGCA GGATCGCAAC
    ATTTTCGAGC ACACATATTT CAACTTTATG TGGCAGATAT GTGGGCACAT ACCCCAATCA
    TTAATATCAG AGACGGACGT CACCTGCATG GCAGCCAAGT TGTCTGTGTC CTTTTTCATT
    GAGACTTTTA TTCATGCTAA GGAAAAGCCC ACTATGGTGC CCTGGGTGGA GCTGCTGACC
    AAGCAGTTCA ATGCCAGCCA AGAGGCCTGC GAGTGGTTTC TGTCGCACAT GTCGCAGGAG
    CCCTACTGGC CGGTCCAGGT GCTAATCCAG TGTCCCAACC AAATGGTGCG TCAAATGTTT
    CAGCGTTTGG TAATCCATGT CATCCAGCAA CTACGTGCCT CACATGCTCA TCTGTATCTG
    GAAGTGGAGA CAGACGAGGA TGACAAGGAG TTAATAGGCC AAGCCTCTTG CGTCACCCGT
    TTCATTGGTT CGCTCATCTC GCTCCTGGAG CACGGTGCCC GAGCGAACCT GCGGCACTTG
    TCCGAGTACT TTGGACTGTT GTGCGAGTTC TCACGGATGG GAGATGAGGA AGCCATGTAC
    TTGCTGCGCA TCGGTGTACT CAAGAGCCTG GTCGACTTCT ATCTGGGTCA CAAGCAAACA
    GACAGCATAG ACATAAGCTC TGACAACGAA GACAACTCCT CTGAAGAGGC GCTGTCCGTG
    GAGAAGATGC GTCCGGCCTC GCTGGACAAG ATGATTGCTC TGTGCGCCAG CCTAGTGGAA
    AGATCAAGAG GAGCCGACTT CCGGCTGCGA CTCTCGCCCA AAGACTTCTC TGCAATCGCC
    GGCGGCAAGG GTTTTCCATT TTTGTACCAA CAAATAAAGG ACGGCATTAA TCCGCACCAG
    ACAAAGCATT TAATACACGC GCTTTGTCGC TGGGATGAGC GCCTGGCCAC GCAGATCATT
    GGCATGCTCT TCGCGTCGGT AACCAAGCAC ACGGAGCTGT GCGCGCCATT CTTCAAGCTG
    CTAACTCTGC TCACCGAGAC GCAGGGCGGT CCAGTGGGCC TGCCTTGTTT CACCCAGTTG
    ATCCTGCCAC GAATGTGGGA CGCCGCCGAG TACTGTCCAC AGTCGGTGCT CGACTGGCTC
    TCGCTGCAGG CCACCAAGAA CAAGATCGCC CACGCCTGGA TACTTCAATC GGCCGAGAAG
    TGGCTCGAGC AATTCCTGCT GGCCCACGAT AACACTCGTG TTCGGAACGC TGCCGCCTTT
    CTGCTGGTGG CACTGGTACC CTCCCAGCCA TTCCGGGCCA ACTTCCGCGC CCACTCGCAA
    CACAAGCTGC TGGCCCTCAA TCCGCACAGT TATCGCGACA TAAATAGCGA TGCCCAGGCG
    GTGCTGCACC AGGTGATCAC GCTGCTCCTG CGATTGCTGC GTCCAGCGCG CGTCTATGCG
    GATATCGGAG CCCATGGCAC GACCAAGCTG ACCGCGTACT TTAATCTGCT CAGCTATTGT
    ATGGTTTCAA AGACTGAAAA ACTAATGATA GGTTCGTACA TGCGCTCCCT GTGGGAGCTG
    TTCCATCCAC GCCTCTCGGA GCCCAGTGTT CCGGCCCATC ACAATAAGCA CGCACTATTA
    ACCTTCTGGC ACCACTCACT GGTGGACTGT CCGGAGAATG CTGCCCAGGT GGCCAACTGC
    CCGGAGATTA CGCGCAACAT TGCATTTAAC TATATACTGG CCGATCATGA TGACGCGGAG
    ATAGTCACCT ACAACCGTTC CATGCTGCCC GCCTATTATG GACTACTTCG TCTGTGCTGC
    GAGCAGAGCC GTGCACTCAC ACGCCAATTG TCCCAGCATC AGAATCTTCA GTGGGCATTT
    AAAAACATCA CACCGCATCC CACGCAGTAC GCGGCCGCCG TGGACGAGCT TTTCAAGTTG
    ATGGCTCTGT TCGCCACCCG TCATCCGGAT GCTAGTGAAC AGGAAAAGCT TGATGTGACC
    CAGTTTAGGA GGGCTGTGAT TGTGTCCTAC ACCAGTAGTC TGGATGCACG TGTCTCTTGG
    TCCACGCTGA TCAGTGCTCT TAAGATACTC GTGGACAATG AGGAGGACCG CACGATGGTG
    ATCTTCAACG GCGGCATTGA GATGTGTTTC GAGGCGCTGC ACACGCTACA TTCCATGCAC
    CACGAGGCCA CCGCCTGCCA CGTGGCCGGA GACCTATTCG ATCTCTTGGG CGAGATGCTA
    CTCCTCCTAG CCACTCTTCG AACCCGCACA GACAGTCCTG CTCAAAAGAA GCAGCAGCAA
    CAACAGCAGC AGCTGGAGCA ACAGTTGCAG TTGCAGCAGC AACAGATGCT GCAGAAACAA
    CAGCAGCAGT CGCAAAGCCA AACGCAAACA CCGCAGTCGC CGCAGCAAAA GGAAAAGCAG
    CTGCAGCAAC AAATGCAGCA GCATCTGCAG CTGCAACAGT TGCAGCAAAT GCAGTTCCAG
    CAGCAACACT TCCTGCGCCA GCAACATCAA CACTCGGCGC TGGCAAAGGC ACTGCCGGAT
    GCAGTGAAGC GACTGGCTAC GCTACTCAAC ACCTTCAATT CGCCAGAGAT CAGTCGCATG
    GCCTTGGAGG TGCTCAAGGA GCTGGTGCGC AATCCCAGCC TGGAAACCAT TAGTATTCTG
    GCGCCCATTC TTATCAACTG TCATCTGTCC GTGGCGAATG CTCCCAATGC GATCGGTCCT
    CTGGGACCGT ACTTTCCGCG TCGCGGTGCG AAGCATACGC CTTGGCCATT GGGAGCCAAG
    AACTCACCCC GTCCACCGCG GCCCATGGTA CAGATGTGCA TCGCTCTGGC TGAGCTGACA
    CCACGCGGTC TCGATGCCGA TTACGACGTT CAGGTGGAGT CCTTTTACCG ACCCTACCAT
    GACTTTATCG ATGTCATGAT GCGCATGTGC GTCAACACGG GCACTCTGAA CGACACGCTA
    GTCAAGCTGC AATGCTTGGT GGCCATCGAG TCCACGCCGC TGCACTTCAC CTATTTCCCC
    AAATTCTGGG TGGGCATACA CAACAATGCT CTGACGCACA AATACGTTGA GCTGCTGGTG
    AAAAATCAAC TGTTGGTAGA GTACCTTCAC AATGTCCTTA GGGACGAGCG AAGCATGTTG
    AAGGATGCCT GCGTTCGTGA ATTTCTGGAG CTCTATTATC ACAAGGTTGC CGCCCAGCTG
    CCAGTGGCCC GTATGATCTA CACAATCAAC TATGGTATGC ACTCCAAGGA CGACATTGAT
    GAGCTGTGTG GCGATCTGTT CGCCATCCGA ATCATTGCCC AGGCCACTGG AGTGCCGGCC
    TCAGTGCGCA AGGAGTTGCG AGGATCGCTG AGGGCGCTTC AGAACAAGAG CGAGCGGTTT
    AGGAAGGAAT CGGAGAGGGA TCCATTTCCG AACAAGAAGC AGAAACGTGA CTCGCAAAAG
    ATCAAGGAAA AGGAGCACCC TCAGCCGGAG AGCGAGAAAG AGACCTCAAC GGAGAACGAC
    AAACCATCAG ATGTTTCCAT GGAATCCAGC GGTAACGCGG AGCAAGCAAC GGACAGCACA
    AAACCACCAA CTCCTGCAGG CAGCGACGAT GAGCAGATGG ACAAAACTTC AGTTCCCTCG
    TCCGATGACG AGACAGAGTT GGAGGACGAG CTGCAGCCGA CGCCCAAGCG CAACAAGAAG
    GCTTCGAATA AGAAGACCGC CCAGGATCGT GTCAACGAGG AGCGCGAGAA GCGACTTATC
    ACGCTGATCA CCATGGAATC ATATATCCAG AGCATTTTTG CCATCCTTAA GAGAGATGCC
    AGTGTCCCCA GTTCGGGAGC GACTAAGGAG CCCAGTGAGG AACCGTGTGG CGAGGCATCC
    ACTTCGGCGG CTGCCGCTGT AAAGTTATCT CGTCCCAAAG GAGCCTGTAC TCCGCCGGAG
    CCAATTACCG ACGTAAACGA TACGCGTTGC AACATAGATA CTGAGACAGA GGCGGAGGCG
    GATGAGCAAT CTCCAAAGAC GAGCCAGACA AATGGTAGCC AACAGAACGA ATCCCCGCCA
    GCGGCGACCA GTGCAGACAC TGCACCCGCG AATCCTTCAC CAGCTCCCGC GGCCGCCGTT
    GCGTCCACAT CCCAGGCGGC AAGTCCAACA CAGATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_733022.2
    CDS358..12096
    Translation

    Target ORF information:

    RefSeq Version NM_170158.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster puffyeye (puf), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_170158.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    ATGTGCGAGG TGTGCGCCGA CTTCCAGAAT CTCCTGGAGC TCTATGAGGT GCGCGTGGCC 
    AGCTCGGATC TGAAGTTCCA GCTGCTGCTG AAGAGCGAGA TCGAGACCAC CTTCAACTAC
    ATCCAGTCGT GGCCGCAGCG TCAGTGCATG TGCCTGTACC GGGACACCAA GAACTATGAC
    CGTTTCAACC TGGTAGTGCA GTCGCTCATC TGTCTGACGG TGCAGCATCT GAAGCACATC
    GACCACCTCA TCGACAACTA CAAGCGGCTA ACGGCCAGTG CTGCCCAGGT GGTGGCCCAG
    GCCCAGCAGC AGCGTGAACA GCAGCGGGAC GAAGCGTCAG CCCAGGCAGA GGCAAAGGAA
    TCATCCGCGC CAGCGGAAGA ACCTAAGAAG GAGGAGCCAT CTGGGTCCGC CGGTGAGGAG
    GCTCAGGGCT CCGGCGACGG GCCAGCCAAA AAGCCGCCAG TTGGCCCCTG CACTCCCCCT
    CCCCCGCAAA CGGCCAATCC TCAGCACAAA TCCCACCTGC AGTACACAGA GGAGCCCTGG
    ATCCTGCCCG AGGTGGAGAA ACTGTTGGTG CTCGTTTCAA AGGTGTTCCT GCTCAACTTC
    CCGCTGTACA TCGCCCACAA ACATGGCATG CACTCTCGGC TGGACGACCT GCAGGCTGAG
    GAGGCCCACC ACCTGGCCCT CATCTGCGAT CTTCACGACA ACGACCTGCC CATCTATCTG
    CTGCGGAATG TGAGCCTGTT CTGCAACTCG GGCGGCTTTG GCGCCATGTC GCTGTGCTTC
    GAACATCCCG ATCTGCCAGT GTCCACCGCT CACTCGATGA CCGCTGCCGT CTCAAATGTG
    AAACTTTGGC TGAACTATCA CTGTAATACT CAGCTCTTCG TGCCGCTGCG AAGTCGGATA
    CTGCAGTACA TGTGCAAGCT GTCGGATCAG AGCCTCCGAT CTGCCGCAAC CCGCGCGATG
    GCCGATTTTG TGTGGTCCTC TATGCGAGAC CCACTGGATG TTGCTGTCAA CTTCGACACC
    GAAGGATTGG CCCTGGCCTT TAAGTACTTC ACATCGACCA CGCTAACCAT GCGACTGGCA
    GGCATGGCCC AGATCAACGC ACACATTAAT CTATTTAACG AGATCTGCAC CACCGAAACG
    GTCAACGAAG TGGAGCTGTT CGGGCAGCGC ATCGCGAACT GGCTGACTGA AAATCATATT
    GTACAGCACC TGTTTGGCCC GAATCTGCAC GTAGAAATCG TTAAACAGGC ACACGTGCTG
    CTCAACTTTT TGGCGGTAGA GAACCAGATC TCTGAAGAGG ACATCAAGCT GATCTGGCAA
    GCCACCCAGC TGAAGCACTG CTCAAAGACG ATCTTCGACA TCCTGCCTTC GCTGGTGAAG
    AATCTGACCC CGCGCCCGGC AATGCACCTT TACTCCCTCC TTTGCCGCAT GGATCCCAAG
    GAGCACACTG AGCAGAGCAT ATACATAGCC TCAGCGCTAA CAAAACAGCT GTGGACTCGT
    GATACCTCAC GCAGTCAGAT GAACCTGATG CAAGACCACC TGCTGGGGTC CAACGTCACC
    GCTTCCAGCT CTGACAGCGG CAGCATTGAG GGCAGCAACA CAGAGGACGA CCACGTGGGC
    GCCGACGACA GTAGCATTGC CAGCGGTGGC GGCGGCGGAG GTGTTGGTAA CAAGAGTCCA
    ATCGATGGAG TTACTCCATG CAAGCAGGCC CGGCACAGGC GGCACATCTG TGACCCCACC
    ACCGAGAAGG GAAAGCAAAT AAGTCCCGAG GATATGGCCA AAGTAGACTT GGTGAACAAA
    CGGATTGTGA ACATTATCGA CAACACAAGC AGTGAAGAGG AGCTGCAATC GCGGGCCGCA
    CTGGAGCTGC AACTGCATCG CAGCCGCAAA AAGACGAGCA ACAAGCGACG GCGACAAAAA
    ACCAACAAAC AGATCATTCT GCCCCATGAG CTGGTCGAGA TTTGGGACGG AGTTGAAGAT
    GTGCCCAGCA GTGACGAGGC TGATGGCGAA GCCGATGGTG ATGGCGAGGG CGAACTTTTA
    GCGGACAGCG ATGAGTGCAG CGATGGTGCT ACGAGTCAGC TGGTACCCGA CGCCGTGCTA
    AAGCATCTGC AGGGAGAGGG ACCTTTTATC AGGGCCATCG AGACCAATAT CAATGAGCTG
    CTCAGTGGTG CCGAGAACGA TGGCAGTTAC TCATCCCCGA TGAGTAACAA ATCTGAAAAG
    AACCTGGCTG ACTTCGATGA CGAGGACGTG TCGCCTTGCG AGGAGGAGCT GGCCCAGCTG
    GTCAGCTCCC GCGCCAATTG TTCGGACGTT CCACCAGCCT TTGCAGCCGC TGCCGCCGCC
    ATGATGGTGG CCCAGTCGGC GATGGCCCTT CAGAAGTCTG GAGAGTCGAA TGTGGCTGCT
    GCTGCCGCTG CTGTGGCCGC TGCTGCAGCG GCTACCGGAA CTGCACAGCA AACGCTGGTG
    GCAATGAACC GGCAGGCCAG CGTTGCTGCA GCTGCCGCCG TGGTTGCTGC GGCCAAGGCA
    AAATCGGACT CAGATGTCGA TCTGATGGAT GTGGTTTCCG GCGGAAAGCA GCACCATTCA
    CCAAAGGCGA GCCAGGGCAG CTCTACGTCC GGGAGTACAC CGGTGCAGCC CTCGTTCAAG
    CTAAATGATG TCTGCCAGCC CGGAAACACA CTGCTTTGGG ATCTGCTTCA AGACGATAAA
    ATTGGCCAAC TAGGCGAGTC TCTGGCTCTA GAGGCGGAGA AAGCTCTGGC CACTCTCCTG
    TGCTTCAGCA TGGACCGCCA ACTGCGAACG AAATTCATCG AGGGTTGTCT GTATAACGTC
    GCCAACAACC GGAGTGTTAT TGTCAGTCTG CGACTGCTGC CCAAACTGTT CGCCTCGTTC
    CAACAGTTTC GACCCTCGGA CACGCACTCT ATGACGATGT GGGCAGAGCG AAATCACCGG
    ATGATGCAGT GCTTTTTCAA TAATATACGT CACTATGCGC GTCGCCATGC CGAGGTACTG
    ATCACCCAAA ACGGAGAGCA GCAGCAGCAG CAGCTGGGCG GACAACTCTA CTCGCACAAG
    ACACAAGTTT CTGTAAGACT GCAGTTCCTA TCATCGATAT TCTCGACGGT GGGATCCCCG
    AAAAGCTTCC GCCTAACGCT GGAGCAGCTT GACGCGCTAT GGGAGTGGCT GGCCCACGAT
    CCGGAGTGTG CCGACTGCTA CTTTTCGTGG CTGCAGGCCC AGGCCAAAGG CGGTGATCAG
    CATGCTCTGG GCATTGAAGC ACTTCAGCAT TTGTATTTGA AGAAACTCCC AGAGCTGCGA
    CCCGAGGAGT TCTCTATGGT TGCGTTGGGA CTATTTCAAC AGCTCTGTAG CTTCGCCCGC
    ATCGCCATGG CCGAGTACGA CAATCATAGC GACCAGATCT CGGCGAGCGC CAGTGCCGTT
    GGCATGTACC ATCTTTGGAA GATAGCGTTG CGTGCCCAGA GCAACGATGT CTCCTTGGCG
    GCCATACAGT ACATCAACAT GTACTACATG GGTCAACAAT TGCGCCTGGA GAAGGAGTTC
    GTTTCACAGT GTATGGAAAA CCTAGTTCAA GCGGCCACGG CTTTGGAAAG CATCGATGAT
    GAGAATGCTT TGATGCGTGT CCAGCGTGGC CTGCTCTTGC TGAACACCCA TTTGGATACG
    TTCCGGCGTC GATATGCGTT TCATCTGCGC CGCTGGGCCA TCGAAGGCAA AGGGATTGGA
    TCGCACAGTA ACCTTAAAAA TGAGGGAGCG GGTCCACCAC TACGAATAGT GCTGCAACAG
    GCAGGACTTT CGGAAAAAAG CCTTTTGCAG ATGCACGCGT GTGACTTAAT CGCCGACCTC
    AAGGCTGAGG TGTCCAAGTG GTGGGAGAGC CTTCAAACTG GACTAGCTGC TCCTGTGCTC
    GGTCTGCTGC TCAGCGATGG ACCGCTACGC ATTATCACAC AGGGACAGGA ATTGACCTCG
    GATTACGATG AACGGAGTCT CGGAGATGCT GGTTTCAAGG ATAACCAGAT CGTCTACGTA
    TCGTTAGGTG GACGTGGAGC CAGGCGAAAG GAATCCAATT TGGAGCATCC GTCAATGCTG
    CCGCCCCCAC CAAAGGAGTG TTTGCCCACG GTGCTCCTGC TGCAGCCAAA GTACTTCGAG
    AAGCTATTTT GCCTGATGCA GACCCTGGGC GATATGCAAC CGCAAGCGTC GACTGTAAAT
    CCCCAGCACC ACACAAAGGC GCAATTGCTC TCGCGACGCG TATGGGATAT CCTCGCCATG
    CTGCCAACTA ATCCGCACAT TCTAGACGCC TTTAAGAGTC TGGTTACGGA CCTAAGCGAA
    CTCGAGCAGC TTGATGCTGG CGGAGAGGAG GAGCAACTGG CCACCAAGCG CAAGCAAATC
    AAGCAGAAGT TCCGAGATCT TCTCGACCCG AACAACTTGC AAAAGTTCAT GTACTCCCTC
    CATATTGTGG AGAGTCTGGC CCTAACAAGT TCCAGACGTG GTGAATCCAA CGGCAATGTG
    GCCATGGGAA ATACACCGGA ACAGGTACGG GTGAAGAAAT CCTCGATGGG AAGACGGAGA
    AATAGCAACG AGCAGCCGCC ACCGCCGCCG CCAGAAGTCA AGATGAGCAA GGAGCAACTG
    TGCGAGCTGG AGGCCCCACT GACGCCCACA CCCTCGACTG GCTTGCAGGA TGTGGAAACG
    GAGGCCAGCA GTAGCAGCGG TGGCGACAAA GAAAACCAAC CGAAACAACA CAGCAAGCGT
    CAGAAGAAAG GCGAAACTTT TGAACAGGAA AAGGAGCGCC CAGTGGGTTG CTCCACGCCG
    CCGTCTCCAA CTCCTCCGCC ACCAGCACTT TCGGTAGTGG AAAGAGGTGA CAATAAGTGG
    AGTGAGGCGT TCGTCAAATG TGGTGGCCTT CGTCATCTGT ACGAAATCTT TAGTGAGGGC
    CAACTACAAC AAAGCGCACA TCCCAAGGAG TTGGCTTTAA ATGAATGGCG ACACGATTGC
    CTGGCCAGCC TGCTGCGCAT TCTCTGGCTT CTTGGATTCG AGGAACTGCA GTCGGCGGAT
    GCCCATGTTC TAATGTCACG TCCGCACCCG TTTATGCTAC AGTTGATGGA GGTACCACAG
    TGCCTGACCC GACTCTCATC GATCCTTAAC GACGAAGTTC ACCAGCAGCA TCAGGCTTCT
    TCGGCCAATC CACTGGTATT CCCCTATCAG TTCCAGCACT TGCGTACTGG CTTTTGGGGC
    AGGGCCCAGC TCATCCAGTT CGCTATGAAC ATTCTAGTGA GTTTTGTGCA CGCCTCAGCG
    GAAGCGAGAA GGCTTCTCTG GGCCCCAACT GGTACCGATC ACTGTCGATG GCTGCAGAAG
    TTCATCCTGG AGGATCCCGA ACCGGCGGTA CGGCGTGAGA TATGCGCTGG TCTTTATCGC
    ATTTGCTTAG GAAACGCACA TAGCTATCGC CTCTTACTAG CACCACTGCT GCACAAGCTC
    ATCGCCCTGT TGCCATTGGC CGAGCAGATG AGCTCTGGCA ACCAGCACAC GCAGTTTTTG
    CTCTCGGAAG AGGGCAAGGA TCCCTACGGA CCCGCCTGCC GTGACTACTT TTGGCTGCTG
    GCCAGATTGG TGGATACTCT CTCCCCGGAA ATGGTGGCCG AGGAGCACAT CGACATTGAA
    ATGCTGTGCG AAAGCATCTC GCAGAGCATT CTCACCCGAG AGTATTATGA ACTGCGCCAT
    GGTTACCAGG ACGATGGCTT GGTGGGTCTC CTAAATCTCA TGTCCAATTT GATCAAGTAC
    GACACCACAT TCAAGTACAC ACCCAAGGCG TTATCCTTTA TTGAGCAACT GATTGGCTTC
    CTGTTTGACA TGCCCTCGCC GGCAGATCGC CAGAAGCCCA AGTGCAAGTC GGCGTCGTCT
    CGAGCATCTG CCTACGATCT GCTGGTTGAA CTTTGCCGTG GATGCGCCAC AAACTATGCA
    TATCTGCATG GACGTCTGTT GGCACAGCAC AAATCCGGAC CCAAGCAGCC GTATCCCTGG
    GACTATTGGC CGCGCGATGA AGGCAGAGCT GAGTGCGGAT ATGTGGGGCT AACTAATTTG
    GGAGCCACTT GCTATATGGC CTCTTGCGTC CAGCACTTGT ACATGATGCC ACAGGCGCGA
    GCAGCTGTTT TGCGAGTTCC ACCCAACGCC GCCCGTAAGC ATGGGCCCAC ACTTCTGGAA
    CTACAACGGA TGTTTGCCTA CCTACTGGAG TCGGAACGGA AGTCTTACAA CCCGCGAAGC
    TTCTGCCGGG TGTACCAGAT GGATCACCAG CCGCTAAATA CGGGAGAGCA AAAGGATATG
    GCAGAGTTCT TTATCGATTT GGTGTCCAAA CTGGAAGATA TGACGCCTGA CTTGAAGCAC
    CTGGTGAAAA GGCTCTTCTG TGGATCCTTG AGCAATAATG TCGTCTCCCT GGATTGCGGT
    CACGTTTCCC GCACGGCCGA GGACTTTTAC ACGGTTCGCT GCCAGGTTGC AGATATGCGC
    AATCTGCAGG AATCCCTGGA CGAAGTCACC GTTAAGGATA CCCTCGAGGG CGACAACATG
    TACACCTGCT CGCAGTGCGG CAAAAAAGTA CGAGCCGAGA AGCGGGCTTG CTTCAAGAAG
    CTTCCCCAGA TCTTGTGCTT CAACACCATG CGCTACACCT TCAATATGGT CACCATGCTC
    AAGGAGAAGG TCAACACGCA CTTTTCCTTT CCGCTACGCC TGAACATGTG TCACTATGTG
    GAGAAGACCT TGATGCCGCA GCAGTACAAA GAGGAGCGAG AAAGGCGCCA AAAGGAGAAG
    GAAGGAGCAG ATGGTAGTGG CGATGGGAAT GACAACGAAA AGGCAGAGGC CACGCTAGAT
    GACGATATTG AAGAATGCTA CGAGTACGAA CTGGTGGGCG TCACTGTCCA CACGGGCACA
    GCGGATGGCG GCCACTACTA CAGCTTTATA AAGGAGCGGA CGAAGACTAG TTACCATACG
    CACGAGCGGT GGTTTCTTTT CAACGATGCC GAAGTGAAGC CTTTCGATCC CTCACAGATA
    GCGGCGGAGT GCTTTGGCGG CGAGATGACG AGCAAAACCT ACGACTCAGT GACGGAGAAA
    TATCTGGACT TTAGTTTCGA GAAGACCAAC TCTGCCTACA TGCTGTTCTA CGAGCGCCGA
    CTGCCAGAGC ATCTGCAGCG CCGGCATTCC GAGCTGCTGG TTACACCCAC GCCCAGTCCC
    ACAGTGGAAG AGAAGTCCGA GGCGGAGGAG CCCACTAAAA TGGAGACAAG CTCTAGCGAA
    ATCAAGGCAG ATGTGGATGT GGAAGTGGAA GTTGAGGAGA AGGACAAGGA GAAGCCTGCG
    CAGACTGACA CAGAATCTAA GGAAACGCCT GCAAAAGAGG AGATCGCGGA TGACAAATCG
    AAGCAGGATG AGCCTGAGGA AAAGAAAATC GAAAAGCAAT CCAGAGAGGG CGAAGAGAAA
    TCGGAAACGG ACGAAAAGCC CACGGAGATG ACGACCGTGA GCACTGAGGA GGAAAAGCAG
    CCAACAGCCA ATTGTGATAA TCACCAGCAA AACAACAATA GCAACAGCAA AGCCAGCAAC
    GATCAGCAAC CATCCACGTC GAAGGCTGCC CAGAAGCTAC AACTATTTCG TCCATTGTTG
    AACAAGGAGC TCGAGGATTG GATTTGGCAG GACAATCGAC AGTTCCTGCA GGATCGCAAC
    ATTTTCGAGC ACACATATTT CAACTTTATG TGGCAGATAT GTGGGCACAT ACCCCAATCA
    TTAATATCAG AGACGGACGT CACCTGCATG GCAGCCAAGT TGTCTGTGTC CTTTTTCATT
    GAGACTTTTA TTCATGCTAA GGAAAAGCCC ACTATGGTGC CCTGGGTGGA GCTGCTGACC
    AAGCAGTTCA ATGCCAGCCA AGAGGCCTGC GAGTGGTTTC TGTCGCACAT GTCGCAGGAG
    CCCTACTGGC CGGTCCAGGT GCTAATCCAG TGTCCCAACC AAATGGTGCG TCAAATGTTT
    CAGCGTTTGG TAATCCATGT CATCCAGCAA CTACGTGCCT CACATGCTCA TCTGTATCTG
    GAAGTGGAGA CAGACGAGGA TGACAAGGAG TTAATAGGCC AAGCCTCTTG CGTCACCCGT
    TTCATTGGTT CGCTCATCTC GCTCCTGGAG CACGGTGCCC GAGCGAACCT GCGGCACTTG
    TCCGAGTACT TTGGACTGTT GTGCGAGTTC TCACGGATGG GAGATGAGGA AGCCATGTAC
    TTGCTGCGCA TCGGTGTACT CAAGAGCCTG GTCGACTTCT ATCTGGGTCA CAAGCAAACA
    GACAGCATAG ACATAAGCTC TGACAACGAA GACAACTCCT CTGAAGAGGC GCTGTCCGTG
    GAGAAGATGC GTCCGGCCTC GCTGGACAAG ATGATTGCTC TGTGCGCCAG CCTAGTGGAA
    AGATCAAGAG GAGCCGACTT CCGGCTGCGA CTCTCGCCCA AAGACTTCTC TGCAATCGCC
    GGCGGCAAGG GTTTTCCATT TTTGTACCAA CAAATAAAGG ACGGCATTAA TCCGCACCAG
    ACAAAGCATT TAATACACGC GCTTTGTCGC TGGGATGAGC GCCTGGCCAC GCAGATCATT
    GGCATGCTCT TCGCGTCGGT AACCAAGCAC ACGGAGCTGT GCGCGCCATT CTTCAAGCTG
    CTAACTCTGC TCACCGAGAC GCAGGGCGGT CCAGTGGGCC TGCCTTGTTT CACCCAGTTG
    ATCCTGCCAC GAATGTGGGA CGCCGCCGAG TACTGTCCAC AGTCGGTGCT CGACTGGCTC
    TCGCTGCAGG CCACCAAGAA CAAGATCGCC CACGCCTGGA TACTTCAATC GGCCGAGAAG
    TGGCTCGAGC AATTCCTGCT GGCCCACGAT AACACTCGTG TTCGGAACGC TGCCGCCTTT
    CTGCTGGTGG CACTGGTACC CTCCCAGCCA TTCCGGGCCA ACTTCCGCGC CCACTCGCAA
    CACAAGCTGC TGGCCCTCAA TCCGCACAGT TATCGCGACA TAAATAGCGA TGCCCAGGCG
    GTGCTGCACC AGGTGATCAC GCTGCTCCTG CGATTGCTGC GTCCAGCGCG CGTCTATGCG
    GATATCGGAG CCCATGGCAC GACCAAGCTG ACCGCGTACT TTAATCTGCT CAGCTATTGT
    ATGGTTTCAA AGACTGAAAA ACTAATGATA GGTTCGTACA TGCGCTCCCT GTGGGAGCTG
    TTCCATCCAC GCCTCTCGGA GCCCAGTGTT CCGGCCCATC ACAATAAGCA CGCACTATTA
    ACCTTCTGGC ACCACTCACT GGTGGACTGT CCGGAGAATG CTGCCCAGGT GGCCAACTGC
    CCGGAGATTA CGCGCAACAT TGCATTTAAC TATATACTGG CCGATCATGA TGACGCGGAG
    ATAGTCACCT ACAACCGTTC CATGCTGCCC GCCTATTATG GACTACTTCG TCTGTGCTGC
    GAGCAGAGCC GTGCACTCAC ACGCCAATTG TCCCAGCATC AGAATCTTCA GTGGGCATTT
    AAAAACATCA CACCGCATCC CACGCAGTAC GCGGCCGCCG TGGACGAGCT TTTCAAGTTG
    ATGGCTCTGT TCGCCACCCG TCATCCGGAT GCTAGTGAAC AGGAAAAGCT TGATGTGACC
    CAGTTTAGGA GGGCTGTGAT TGTGTCCTAC ACCAGTAGTC TGGATGCACG TGTCTCTTGG
    TCCACGCTGA TCAGTGCTCT TAAGATACTC GTGGACAATG AGGAGGACCG CACGATGGTG
    ATCTTCAACG GCGGCATTGA GATGTGTTTC GAGGCGCTGC ACACGCTACA TTCCATGCAC
    CACGAGGCCA CCGCCTGCCA CGTGGCCGGA GACCTATTCG ATCTCTTGGG CGAGATGCTA
    CTCCTCCTAG CCACTCTTCG AACCCGCACA GACAGTCCTG CTCAAAAGAA GCAGCAGCAA
    CAACAGCAGC AGCTGGAGCA ACAGTTGCAG TTGCAGCAGC AACAGATGCT GCAGAAACAA
    CAGCAGCAGT CGCAAAGCCA AACGCAAACA CCGCAGTCGC CGCAGCAAAA GGAAAAGCAG
    CTGCAGCAAC AAATGCAGCA GCATCTGCAG CTGCAACAGT TGCAGCAAAT GCAGTTCCAG
    CAGCAACACT TCCTGCGCCA GCAACATCAA CACTCGGCGC TGGCAAAGGC ACTGCCGGAT
    GCAGTGAAGC GACTGGCTAC GCTACTCAAC ACCTTCAATT CGCCAGAGAT CAGTCGCATG
    GCCTTGGAGG TGCTCAAGGA GCTGGTGCGC AATCCCAGCC TGGAAACCAT TAGTATTCTG
    GCGCCCATTC TTATCAACTG TCATCTGTCC GTGGCGAATG CTCCCAATGC GATCGGTCCT
    CTGGGACCGT ACTTTCCGCG TCGCGGTGCG AAGCATACGC CTTGGCCATT GGGAGCCAAG
    AACTCACCCC GTCCACCGCG GCCCATGGTA CAGATGTGCA TCGCTCTGGC TGAGCTGACA
    CCACGCGGTC TCGATGCCGA TTACGACGTT CAGGTGGAGT CCTTTTACCG ACCCTACCAT
    GACTTTATCG ATGTCATGAT GCGCATGTGC GTCAACACGG GCACTCTGAA CGACACGCTA
    GTCAAGCTGC AATGCTTGGT GGCCATCGAG TCCACGCCGC TGCACTTCAC CTATTTCCCC
    AAATTCTGGG TGGGCATACA CAACAATGCT CTGACGCACA AATACGTTGA GCTGCTGGTG
    AAAAATCAAC TGTTGGTAGA GTACCTTCAC AATGTCCTTA GGGACGAGCG AAGCATGTTG
    AAGGATGCCT GCGTTCGTGA ATTTCTGGAG CTCTATTATC ACAAGGTTGC CGCCCAGCTG
    CCAGTGGCCC GTATGATCTA CACAATCAAC TATGGTATGC ACTCCAAGGA CGACATTGAT
    GAGCTGTGTG GCGATCTGTT CGCCATCCGA ATCATTGCCC AGGCCACTGG AGTGCCGGCC
    TCAGTGCGCA AGGAGTTGCG AGGATCGCTG AGGGCGCTTC AGAACAAGAG CGAGCGGTTT
    AGGAAGGAAT CGGAGAGGGA TCCATTTCCG AACAAGAAGC AGAAACGTGA CTCGCAAAAG
    ATCAAGGAAA AGGAGCACCC TCAGCCGGAG AGCGAGAAAG AGACCTCAAC GGAGAACGAC
    AAACCATCAG ATGTTTCCAT GGAATCCAGC GGTAACGCGG AGCAAGCAAC GGACAGCACA
    AAACCACCAA CTCCTGCAGG CAGCGACGAT GAGCAGATGG ACAAAACTTC AGTTCCCTCG
    TCCGATGACG AGACAGAGTT GGAGGACGAG CTGCAGCCGA CGCCCAAGCG CAACAAGAAG
    GCTTCGAATA AGAAGACCGC CCAGGATCGT GTCAACGAGG AGCGCGAGAA GCGACTTATC
    ACGCTGATCA CCATGGAATC ATATATCCAG AGCATTTTTG CCATCCTTAA GAGAGATGCC
    AGTGTCCCCA GTTCGGGAGC GACTAAGGAG CCCAGTGAGG AACCGTGTGG CGAGGCATCC
    ACTTCGGCGG CTGCCGCTGT AAAGTTATCT CGTCCCAAAG GAGCCTGTAC TCCGCCGGAG
    CCAATTACCG ACGTAAACGA TACGCGTTGC AACATAGATA CTGAGACAGA GGCGGAGGCG
    GATGAGCAAT CTCCAAAGAC GAGCCAGACA AATGGTAGCC AACAGAACGA ATCCCCGCCA
    GCGGCGACCA GTGCAGACAC TGCACCCGCG AATCCTTCAC CAGCTCCCGC GGCCGCCGTT
    GCGTCCACAT CCCAGGCGGC AAGTCCAACA CAGATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa05978
    Clone ID Related Accession (Same CDS sequence) NM_143018.4
    Accession Version NM_143018.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11739bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product puffyeye, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_143018.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    ATGTGCGAGG TGTGCGCCGA CTTCCAGAAT CTCCTGGAGC TCTATGAGGT GCGCGTGGCC 
    AGCTCGGATC TGAAGTTCCA GCTGCTGCTG AAGAGCGAGA TCGAGACCAC CTTCAACTAC
    ATCCAGTCGT GGCCGCAGCG TCAGTGCATG TGCCTGTACC GGGACACCAA GAACTATGAC
    CGTTTCAACC TGGTAGTGCA GTCGCTCATC TGTCTGACGG TGCAGCATCT GAAGCACATC
    GACCACCTCA TCGACAACTA CAAGCGGCTA ACGGCCAGTG CTGCCCAGGT GGTGGCCCAG
    GCCCAGCAGC AGCGTGAACA GCAGCGGGAC GAAGCGTCAG CCCAGGCAGA GGCAAAGGAA
    TCATCCGCGC CAGCGGAAGA ACCTAAGAAG GAGGAGCCAT CTGGGTCCGC CGGTGAGGAG
    GCTCAGGGCT CCGGCGACGG GCCAGCCAAA AAGCCGCCAG TTGGCCCCTG CACTCCCCCT
    CCCCCGCAAA CGGCCAATCC TCAGCACAAA TCCCACCTGC AGTACACAGA GGAGCCCTGG
    ATCCTGCCCG AGGTGGAGAA ACTGTTGGTG CTCGTTTCAA AGGTGTTCCT GCTCAACTTC
    CCGCTGTACA TCGCCCACAA ACATGGCATG CACTCTCGGC TGGACGACCT GCAGGCTGAG
    GAGGCCCACC ACCTGGCCCT CATCTGCGAT CTTCACGACA ACGACCTGCC CATCTATCTG
    CTGCGGAATG TGAGCCTGTT CTGCAACTCG GGCGGCTTTG GCGCCATGTC GCTGTGCTTC
    GAACATCCCG ATCTGCCAGT GTCCACCGCT CACTCGATGA CCGCTGCCGT CTCAAATGTG
    AAACTTTGGC TGAACTATCA CTGTAATACT CAGCTCTTCG TGCCGCTGCG AAGTCGGATA
    CTGCAGTACA TGTGCAAGCT GTCGGATCAG AGCCTCCGAT CTGCCGCAAC CCGCGCGATG
    GCCGATTTTG TGTGGTCCTC TATGCGAGAC CCACTGGATG TTGCTGTCAA CTTCGACACC
    GAAGGATTGG CCCTGGCCTT TAAGTACTTC ACATCGACCA CGCTAACCAT GCGACTGGCA
    GGCATGGCCC AGATCAACGC ACACATTAAT CTATTTAACG AGATCTGCAC CACCGAAACG
    GTCAACGAAG TGGAGCTGTT CGGGCAGCGC ATCGCGAACT GGCTGACTGA AAATCATATT
    GTACAGCACC TGTTTGGCCC GAATCTGCAC GTAGAAATCG TTAAACAGGC ACACGTGCTG
    CTCAACTTTT TGGCGGTAGA GAACCAGATC TCTGAAGAGG ACATCAAGCT GATCTGGCAA
    GCCACCCAGC TGAAGCACTG CTCAAAGACG ATCTTCGACA TCCTGCCTTC GCTGGTGAAG
    AATCTGACCC CGCGCCCGGC AATGCACCTT TACTCCCTCC TTTGCCGCAT GGATCCCAAG
    GAGCACACTG AGCAGAGCAT ATACATAGCC TCAGCGCTAA CAAAACAGCT GTGGACTCGT
    GATACCTCAC GCAGTCAGAT GAACCTGATG CAAGACCACC TGCTGGGGTC CAACGTCACC
    GCTTCCAGCT CTGACAGCGG CAGCATTGAG GGCAGCAACA CAGAGGACGA CCACGTGGGC
    GCCGACGACA GTAGCATTGC CAGCGGTGGC GGCGGCGGAG GTGTTGGTAA CAAGAGTCCA
    ATCGATGGAG TTACTCCATG CAAGCAGGCC CGGCACAGGC GGCACATCTG TGACCCCACC
    ACCGAGAAGG GAAAGCAAAT AAGTCCCGAG GATATGGCCA AAGTAGACTT GGTGAACAAA
    CGGATTGTGA ACATTATCGA CAACACAAGC AGTGAAGAGG AGCTGCAATC GCGGGCCGCA
    CTGGAGCTGC AACTGCATCG CAGCCGCAAA AAGACGAGCA ACAAGCGACG GCGACAAAAA
    ACCAACAAAC AGATCATTCT GCCCCATGAG CTGGTCGAGA TTTGGGACGG AGTTGAAGAT
    GTGCCCAGCA GTGACGAGGC TGATGGCGAA GCCGATGGTG ATGGCGAGGG CGAACTTTTA
    GCGGACAGCG ATGAGTGCAG CGATGGTGCT ACGAGTCAGC TGGTACCCGA CGCCGTGCTA
    AAGCATCTGC AGGGAGAGGG ACCTTTTATC AGGGCCATCG AGACCAATAT CAATGAGCTG
    CTCAGTGGTG CCGAGAACGA TGGCAGTTAC TCATCCCCGA TGAGTAACAA ATCTGAAAAG
    AACCTGGCTG ACTTCGATGA CGAGGACGTG TCGCCTTGCG AGGAGGAGCT GGCCCAGCTG
    GTCAGCTCCC GCGCCAATTG TTCGGACGTT CCACCAGCCT TTGCAGCCGC TGCCGCCGCC
    ATGATGGTGG CCCAGTCGGC GATGGCCCTT CAGAAGTCTG GAGAGTCGAA TGTGGCTGCT
    GCTGCCGCTG CTGTGGCCGC TGCTGCAGCG GCTACCGGAA CTGCACAGCA AACGCTGGTG
    GCAATGAACC GGCAGGCCAG CGTTGCTGCA GCTGCCGCCG TGGTTGCTGC GGCCAAGGCA
    AAATCGGACT CAGATGTCGA TCTGATGGAT GTGGTTTCCG GCGGAAAGCA GCACCATTCA
    CCAAAGGCGA GCCAGGGCAG CTCTACGTCC GGGAGTACAC CGGTGCAGCC CTCGTTCAAG
    CTAAATGATG TCTGCCAGCC CGGAAACACA CTGCTTTGGG ATCTGCTTCA AGACGATAAA
    ATTGGCCAAC TAGGCGAGTC TCTGGCTCTA GAGGCGGAGA AAGCTCTGGC CACTCTCCTG
    TGCTTCAGCA TGGACCGCCA ACTGCGAACG AAATTCATCG AGGGTTGTCT GTATAACGTC
    GCCAACAACC GGAGTGTTAT TGTCAGTCTG CGACTGCTGC CCAAACTGTT CGCCTCGTTC
    CAACAGTTTC GACCCTCGGA CACGCACTCT ATGACGATGT GGGCAGAGCG AAATCACCGG
    ATGATGCAGT GCTTTTTCAA TAATATACGT CACTATGCGC GTCGCCATGC CGAGGTACTG
    ATCACCCAAA ACGGAGAGCA GCAGCAGCAG CAGCTGGGCG GACAACTCTA CTCGCACAAG
    ACACAAGTTT CTGTAAGACT GCAGTTCCTA TCATCGATAT TCTCGACGGT GGGATCCCCG
    AAAAGCTTCC GCCTAACGCT GGAGCAGCTT GACGCGCTAT GGGAGTGGCT GGCCCACGAT
    CCGGAGTGTG CCGACTGCTA CTTTTCGTGG CTGCAGGCCC AGGCCAAAGG CGGTGATCAG
    CATGCTCTGG GCATTGAAGC ACTTCAGCAT TTGTATTTGA AGAAACTCCC AGAGCTGCGA
    CCCGAGGAGT TCTCTATGGT TGCGTTGGGA CTATTTCAAC AGCTCTGTAG CTTCGCCCGC
    ATCGCCATGG CCGAGTACGA CAATCATAGC GACCAGATCT CGGCGAGCGC CAGTGCCGTT
    GGCATGTACC ATCTTTGGAA GATAGCGTTG CGTGCCCAGA GCAACGATGT CTCCTTGGCG
    GCCATACAGT ACATCAACAT GTACTACATG GGTCAACAAT TGCGCCTGGA GAAGGAGTTC
    GTTTCACAGT GTATGGAAAA CCTAGTTCAA GCGGCCACGG CTTTGGAAAG CATCGATGAT
    GAGAATGCTT TGATGCGTGT CCAGCGTGGC CTGCTCTTGC TGAACACCCA TTTGGATACG
    TTCCGGCGTC GATATGCGTT TCATCTGCGC CGCTGGGCCA TCGAAGGCAA AGGGATTGGA
    TCGCACAGTA ACCTTAAAAA TGAGGGAGCG GGTCCACCAC TACGAATAGT GCTGCAACAG
    GCAGGACTTT CGGAAAAAAG CCTTTTGCAG ATGCACGCGT GTGACTTAAT CGCCGACCTC
    AAGGCTGAGG TGTCCAAGTG GTGGGAGAGC CTTCAAACTG GACTAGCTGC TCCTGTGCTC
    GGTCTGCTGC TCAGCGATGG ACCGCTACGC ATTATCACAC AGGGACAGGA ATTGACCTCG
    GATTACGATG AACGGAGTCT CGGAGATGCT GGTTTCAAGG ATAACCAGAT CGTCTACGTA
    TCGTTAGGTG GACGTGGAGC CAGGCGAAAG GAATCCAATT TGGAGCATCC GTCAATGCTG
    CCGCCCCCAC CAAAGGAGTG TTTGCCCACG GTGCTCCTGC TGCAGCCAAA GTACTTCGAG
    AAGCTATTTT GCCTGATGCA GACCCTGGGC GATATGCAAC CGCAAGCGTC GACTGTAAAT
    CCCCAGCACC ACACAAAGGC GCAATTGCTC TCGCGACGCG TATGGGATAT CCTCGCCATG
    CTGCCAACTA ATCCGCACAT TCTAGACGCC TTTAAGAGTC TGGTTACGGA CCTAAGCGAA
    CTCGAGCAGC TTGATGCTGG CGGAGAGGAG GAGCAACTGG CCACCAAGCG CAAGCAAATC
    AAGCAGAAGT TCCGAGATCT TCTCGACCCG AACAACTTGC AAAAGTTCAT GTACTCCCTC
    CATATTGTGG AGAGTCTGGC CCTAACAAGT TCCAGACGTG GTGAATCCAA CGGCAATGTG
    GCCATGGGAA ATACACCGGA ACAGGTACGG GTGAAGAAAT CCTCGATGGG AAGACGGAGA
    AATAGCAACG AGCAGCCGCC ACCGCCGCCG CCAGAAGTCA AGATGAGCAA GGAGCAACTG
    TGCGAGCTGG AGGCCCCACT GACGCCCACA CCCTCGACTG GCTTGCAGGA TGTGGAAACG
    GAGGCCAGCA GTAGCAGCGG TGGCGACAAA GAAAACCAAC CGAAACAACA CAGCAAGCGT
    CAGAAGAAAG GCGAAACTTT TGAACAGGAA AAGGAGCGCC CAGTGGGTTG CTCCACGCCG
    CCGTCTCCAA CTCCTCCGCC ACCAGCACTT TCGGTAGTGG AAAGAGGTGA CAATAAGTGG
    AGTGAGGCGT TCGTCAAATG TGGTGGCCTT CGTCATCTGT ACGAAATCTT TAGTGAGGGC
    CAACTACAAC AAAGCGCACA TCCCAAGGAG TTGGCTTTAA ATGAATGGCG ACACGATTGC
    CTGGCCAGCC TGCTGCGCAT TCTCTGGCTT CTTGGATTCG AGGAACTGCA GTCGGCGGAT
    GCCCATGTTC TAATGTCACG TCCGCACCCG TTTATGCTAC AGTTGATGGA GGTACCACAG
    TGCCTGACCC GACTCTCATC GATCCTTAAC GACGAAGTTC ACCAGCAGCA TCAGGCTTCT
    TCGGCCAATC CACTGGTATT CCCCTATCAG TTCCAGCACT TGCGTACTGG CTTTTGGGGC
    AGGGCCCAGC TCATCCAGTT CGCTATGAAC ATTCTAGTGA GTTTTGTGCA CGCCTCAGCG
    GAAGCGAGAA GGCTTCTCTG GGCCCCAACT GGTACCGATC ACTGTCGATG GCTGCAGAAG
    TTCATCCTGG AGGATCCCGA ACCGGCGGTA CGGCGTGAGA TATGCGCTGG TCTTTATCGC
    ATTTGCTTAG GAAACGCACA TAGCTATCGC CTCTTACTAG CACCACTGCT GCACAAGCTC
    ATCGCCCTGT TGCCATTGGC CGAGCAGATG AGCTCTGGCA ACCAGCACAC GCAGTTTTTG
    CTCTCGGAAG AGGGCAAGGA TCCCTACGGA CCCGCCTGCC GTGACTACTT TTGGCTGCTG
    GCCAGATTGG TGGATACTCT CTCCCCGGAA ATGGTGGCCG AGGAGCACAT CGACATTGAA
    ATGCTGTGCG AAAGCATCTC GCAGAGCATT CTCACCCGAG AGTATTATGA ACTGCGCCAT
    GGTTACCAGG ACGATGGCTT GGTGGGTCTC CTAAATCTCA TGTCCAATTT GATCAAGTAC
    GACACCACAT TCAAGTACAC ACCCAAGGCG TTATCCTTTA TTGAGCAACT GATTGGCTTC
    CTGTTTGACA TGCCCTCGCC GGCAGATCGC CAGAAGCCCA AGTGCAAGTC GGCGTCGTCT
    CGAGCATCTG CCTACGATCT GCTGGTTGAA CTTTGCCGTG GATGCGCCAC AAACTATGCA
    TATCTGCATG GACGTCTGTT GGCACAGCAC AAATCCGGAC CCAAGCAGCC GTATCCCTGG
    GACTATTGGC CGCGCGATGA AGGCAGAGCT GAGTGCGGAT ATGTGGGGCT AACTAATTTG
    GGAGCCACTT GCTATATGGC CTCTTGCGTC CAGCACTTGT ACATGATGCC ACAGGCGCGA
    GCAGCTGTTT TGCGAGTTCC ACCCAACGCC GCCCGTAAGC ATGGGCCCAC ACTTCTGGAA
    CTACAACGGA TGTTTGCCTA CCTACTGGAG TCGGAACGGA AGTCTTACAA CCCGCGAAGC
    TTCTGCCGGG TGTACCAGAT GGATCACCAG CCGCTAAATA CGGGAGAGCA AAAGGATATG
    GCAGAGTTCT TTATCGATTT GGTGTCCAAA CTGGAAGATA TGACGCCTGA CTTGAAGCAC
    CTGGTGAAAA GGCTCTTCTG TGGATCCTTG AGCAATAATG TCGTCTCCCT GGATTGCGGT
    CACGTTTCCC GCACGGCCGA GGACTTTTAC ACGGTTCGCT GCCAGGTTGC AGATATGCGC
    AATCTGCAGG AATCCCTGGA CGAAGTCACC GTTAAGGATA CCCTCGAGGG CGACAACATG
    TACACCTGCT CGCAGTGCGG CAAAAAAGTA CGAGCCGAGA AGCGGGCTTG CTTCAAGAAG
    CTTCCCCAGA TCTTGTGCTT CAACACCATG CGCTACACCT TCAATATGGT CACCATGCTC
    AAGGAGAAGG TCAACACGCA CTTTTCCTTT CCGCTACGCC TGAACATGTG TCACTATGTG
    GAGAAGACCT TGATGCCGCA GCAGTACAAA GAGGAGCGAG AAAGGCGCCA AAAGGAGAAG
    GAAGGAGCAG ATGGTAGTGG CGATGGGAAT GACAACGAAA AGGCAGAGGC CACGCTAGAT
    GACGATATTG AAGAATGCTA CGAGTACGAA CTGGTGGGCG TCACTGTCCA CACGGGCACA
    GCGGATGGCG GCCACTACTA CAGCTTTATA AAGGAGCGGA CGAAGACTAG TTACCATACG
    CACGAGCGGT GGTTTCTTTT CAACGATGCC GAAGTGAAGC CTTTCGATCC CTCACAGATA
    GCGGCGGAGT GCTTTGGCGG CGAGATGACG AGCAAAACCT ACGACTCAGT GACGGAGAAA
    TATCTGGACT TTAGTTTCGA GAAGACCAAC TCTGCCTACA TGCTGTTCTA CGAGCGCCGA
    CTGCCAGAGC ATCTGCAGCG CCGGCATTCC GAGCTGCTGG TTACACCCAC GCCCAGTCCC
    ACAGTGGAAG AGAAGTCCGA GGCGGAGGAG CCCACTAAAA TGGAGACAAG CTCTAGCGAA
    ATCAAGGCAG ATGTGGATGT GGAAGTGGAA GTTGAGGAGA AGGACAAGGA GAAGCCTGCG
    CAGACTGACA CAGAATCTAA GGAAACGCCT GCAAAAGAGG AGATCGCGGA TGACAAATCG
    AAGCAGGATG AGCCTGAGGA AAAGAAAATC GAAAAGCAAT CCAGAGAGGG CGAAGAGAAA
    TCGGAAACGG ACGAAAAGCC CACGGAGATG ACGACCGTGA GCACTGAGGA GGAAAAGCAG
    CCAACAGCCA ATTGTGATAA TCACCAGCAA AACAACAATA GCAACAGCAA AGCCAGCAAC
    GATCAGCAAC CATCCACGTC GAAGGCTGCC CAGAAGCTAC AACTATTTCG TCCATTGTTG
    AACAAGGAGC TCGAGGATTG GATTTGGCAG GACAATCGAC AGTTCCTGCA GGATCGCAAC
    ATTTTCGAGC ACACATATTT CAACTTTATG TGGCAGATAT GTGGGCACAT ACCCCAATCA
    TTAATATCAG AGACGGACGT CACCTGCATG GCAGCCAAGT TGTCTGTGTC CTTTTTCATT
    GAGACTTTTA TTCATGCTAA GGAAAAGCCC ACTATGGTGC CCTGGGTGGA GCTGCTGACC
    AAGCAGTTCA ATGCCAGCCA AGAGGCCTGC GAGTGGTTTC TGTCGCACAT GTCGCAGGAG
    CCCTACTGGC CGGTCCAGGT GCTAATCCAG TGTCCCAACC AAATGGTGCG TCAAATGTTT
    CAGCGTTTGG TAATCCATGT CATCCAGCAA CTACGTGCCT CACATGCTCA TCTGTATCTG
    GAAGTGGAGA CAGACGAGGA TGACAAGGAG TTAATAGGCC AAGCCTCTTG CGTCACCCGT
    TTCATTGGTT CGCTCATCTC GCTCCTGGAG CACGGTGCCC GAGCGAACCT GCGGCACTTG
    TCCGAGTACT TTGGACTGTT GTGCGAGTTC TCACGGATGG GAGATGAGGA AGCCATGTAC
    TTGCTGCGCA TCGGTGTACT CAAGAGCCTG GTCGACTTCT ATCTGGGTCA CAAGCAAACA
    GACAGCATAG ACATAAGCTC TGACAACGAA GACAACTCCT CTGAAGAGGC GCTGTCCGTG
    GAGAAGATGC GTCCGGCCTC GCTGGACAAG ATGATTGCTC TGTGCGCCAG CCTAGTGGAA
    AGATCAAGAG GAGCCGACTT CCGGCTGCGA CTCTCGCCCA AAGACTTCTC TGCAATCGCC
    GGCGGCAAGG GTTTTCCATT TTTGTACCAA CAAATAAAGG ACGGCATTAA TCCGCACCAG
    ACAAAGCATT TAATACACGC GCTTTGTCGC TGGGATGAGC GCCTGGCCAC GCAGATCATT
    GGCATGCTCT TCGCGTCGGT AACCAAGCAC ACGGAGCTGT GCGCGCCATT CTTCAAGCTG
    CTAACTCTGC TCACCGAGAC GCAGGGCGGT CCAGTGGGCC TGCCTTGTTT CACCCAGTTG
    ATCCTGCCAC GAATGTGGGA CGCCGCCGAG TACTGTCCAC AGTCGGTGCT CGACTGGCTC
    TCGCTGCAGG CCACCAAGAA CAAGATCGCC CACGCCTGGA TACTTCAATC GGCCGAGAAG
    TGGCTCGAGC AATTCCTGCT GGCCCACGAT AACACTCGTG TTCGGAACGC TGCCGCCTTT
    CTGCTGGTGG CACTGGTACC CTCCCAGCCA TTCCGGGCCA ACTTCCGCGC CCACTCGCAA
    CACAAGCTGC TGGCCCTCAA TCCGCACAGT TATCGCGACA TAAATAGCGA TGCCCAGGCG
    GTGCTGCACC AGGTGATCAC GCTGCTCCTG CGATTGCTGC GTCCAGCGCG CGTCTATGCG
    GATATCGGAG CCCATGGCAC GACCAAGCTG ACCGCGTACT TTAATCTGCT CAGCTATTGT
    ATGGTTTCAA AGACTGAAAA ACTAATGGCA AGTTCGTACA TGCGCTCCCT GTGGGAGCTG
    TTCCATCCAC GCCTCTCGGA GCCCAGTGTT CCGGCCCATC ACAATAAGCA CGCACTATTA
    ACCTTCTGGC ACCACTCACT GGTGGACTGT CCGGAGAATG CTGCCCAGGT GGCCAACTGC
    CCGGAGATTA CGCGCAACAT TGCATTTAAC TATATACTGG CCGATCATGA TGACGCGGAG
    ATAGTCACCT ACAACCGTTC CATGCTGCCC GCCTATTATG GACTACTTCG TCTGTGCTGC
    GAGCAGAGCC GTGCACTCAC ACGCCAATTG TCCCAGCATC AGAATCTTCA GTGGGCATTT
    AAAAACATCA CACCGCATCC CACGCAGTAC GCGGCCGCCG TGGACGAGCT TTTCAAGTTG
    ATGGCTCTGT TCGCCACCCG TCATCCGGAT GCTAGTGAAC AGGAAAAGCT TGATGTGACC
    CAGTTTAGGA GGGCTGTGAT TGTGTCCTAC ACCAGTAGTC TGGATGCACG TGTCTCTTGG
    TCCACGCTGA TCAGTGCTCT TAAGATACTC GTGGACAATG AGGAGGACCG CACGATGGTG
    ATCTTCAACG GCGGCATTGA GATGTGTTTC GAGGCGCTGC ACACGCTACA TTCCATGCAC
    CACGAGGCCA CCGCCTGCCA CGTGGCCGGA GACCTATTCG ATCTCTTGGG CGAGATGCTA
    CTCCTCCTAG CCACTCTTCG AACCCGCACA GACAGTCCTG CTCAAAAGAA GCAGCAGCAA
    CAACAGCAGC AGCTGGAGCA ACAGTTGCAG TTGCAGCAGC AACAGATGCT GCAGAAACAA
    CAGCAGCAGT CGCAAAGCCA AACGCAAACA CCGCAGTCGC CGCAGCAAAA GGAAAAGCAG
    CTGCAGCAAC AAATGCAGCA GCATCTGCAG CTGCAACAGT TGCAGCAAAT GCAGTTCCAG
    CAGCAACACT TCCTGCGCCA GCAACATCAA CACTCGGCGC TGGCAAAGGC ACTGCCGGAT
    GCAGTGAAGC GACTGGCTAC GCTACTCAAC ACCTTCAATT CGCCAGAGAT CAGTCGCATG
    GCCTTGGAGG TGCTCAAGGA GCTGGTGCGC AATCCCAGCC TGGAAACCAT TAGTATTCTG
    GCGCCCATTC TTATCAACTG TCATCTGTCC GTGGCGAATG CTCCCAATGC GATCGGTCCT
    CTGGGACCGT ACTTTCCGCG TCGCGGTGCG AAGCATACGC CTTGGCCATT GGGAGCCAAG
    AACTCACCCC GTCCACCGCG GCCCATGGTA CAGATGTGCA TCGCTCTGGC TGAGCTGACA
    CCACGCGGTC TCGATGCCGA TTACGACGTT CAGGTGGAGT CCTTTTACCG ACCCTACCAT
    GACTTTATCG ATGTCATGAT GCGCATGTGC GTCAACACGG GCACTCTGAA CGACACGCTA
    GTCAAGCTGC AATGCTTGGT GGCCATCGAG TCCACGCCGC TGCACTTCAC CTATTTCCCC
    AAATTCTGGG TGGGCATACA CAACAATGCT CTGACGCACA AATACGTTGA GCTGCTGGTG
    AAAAATCAAC TGTTGGTAGA GTACCTTCAC AATGTCCTTA GGGACGAGCG AAGCATGTTG
    AAGGATGCCT GCGTTCGTGA ATTTCTGGAG CTCTATTATC ACAAGGTTGC CGCCCAGCTG
    CCAGTGGCCC GTATGATCTA CACAATCAAC TATGGTATGC ACTCCAAGGA CGACATTGAT
    GAGCTGTGTG GCGATCTGTT CGCCATCCGA ATCATTGCCC AGGCCACTGG AGTGCCGGCC
    TCAGTGCGCA AGGAGTTGCG AGGATCGCTG AGGGCGCTTC AGAACAAGAG CGAGCGGTTT
    AGGAAGGAAT CGGAGAGGGA TCCATTTCCG AACAAGAAGC AGAAACGTGA CTCGCAAAAG
    ATCAAGGAAA AGGAGCACCC TCAGCCGGAG AGCGAGAAAG AGACCTCAAC GGAGAACGAC
    AAACCATCAG ATGTTTCCAT GGAATCCAGC GGTAACGCGG AGCAAGCAAC GGACAGCACA
    AAACCACCAA CTCCTGCAGG CAGCGACGAT GAGCAGATGG ACAAAACTTC AGTTCCCTCG
    TCCGATGACG AGACAGAGTT GGAGGACGAG CTGCAGCCGA CGCCCAAGCG CAACAAGAAG
    GCTTCGAATA AGAAGACCGC CCAGGATCGT GTCAACGAGG AGCGCGAGAA GCGACTTATC
    ACGCTGATCA CCATGGAATC ATATATCCAG AGCATTTTTG CCATCCTTAA GAGAGATGCC
    AGTGTCCCCA GTTCGGGAGC GACTAAGGAG CCCAGTGAGG AACCGTGTGG CGAGGCATCC
    ACTTCGGCGG CTGCCGCTGT AAAGTTATCT CGTCCCAAAG GAGCCTGTAC TCCGCCGGAG
    CCAATTACCG ACGTAAACGA TACGCGTTGC AACATAGATA CTGAGACAGA GGCGGAGGCG
    GATGAGCAAT CTCCAAAGAC GAGCCAGACA AATGGTAGCC AACAGAACGA ATCCCCGCCA
    GCGGCGACCA GTGCAGACAC TGCACCCGCG AATCCTTCAC CAGCTCCCGC GGCCGCCGTT
    GCGTCCACAT CCCAGGCGGC AAGTCCAACA CAGATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_651275.1
    CDS358..12096
    Translation

    Target ORF information:

    RefSeq Version NM_143018.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster puffyeye (puf), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_143018.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    ATGTGCGAGG TGTGCGCCGA CTTCCAGAAT CTCCTGGAGC TCTATGAGGT GCGCGTGGCC 
    AGCTCGGATC TGAAGTTCCA GCTGCTGCTG AAGAGCGAGA TCGAGACCAC CTTCAACTAC
    ATCCAGTCGT GGCCGCAGCG TCAGTGCATG TGCCTGTACC GGGACACCAA GAACTATGAC
    CGTTTCAACC TGGTAGTGCA GTCGCTCATC TGTCTGACGG TGCAGCATCT GAAGCACATC
    GACCACCTCA TCGACAACTA CAAGCGGCTA ACGGCCAGTG CTGCCCAGGT GGTGGCCCAG
    GCCCAGCAGC AGCGTGAACA GCAGCGGGAC GAAGCGTCAG CCCAGGCAGA GGCAAAGGAA
    TCATCCGCGC CAGCGGAAGA ACCTAAGAAG GAGGAGCCAT CTGGGTCCGC CGGTGAGGAG
    GCTCAGGGCT CCGGCGACGG GCCAGCCAAA AAGCCGCCAG TTGGCCCCTG CACTCCCCCT
    CCCCCGCAAA CGGCCAATCC TCAGCACAAA TCCCACCTGC AGTACACAGA GGAGCCCTGG
    ATCCTGCCCG AGGTGGAGAA ACTGTTGGTG CTCGTTTCAA AGGTGTTCCT GCTCAACTTC
    CCGCTGTACA TCGCCCACAA ACATGGCATG CACTCTCGGC TGGACGACCT GCAGGCTGAG
    GAGGCCCACC ACCTGGCCCT CATCTGCGAT CTTCACGACA ACGACCTGCC CATCTATCTG
    CTGCGGAATG TGAGCCTGTT CTGCAACTCG GGCGGCTTTG GCGCCATGTC GCTGTGCTTC
    GAACATCCCG ATCTGCCAGT GTCCACCGCT CACTCGATGA CCGCTGCCGT CTCAAATGTG
    AAACTTTGGC TGAACTATCA CTGTAATACT CAGCTCTTCG TGCCGCTGCG AAGTCGGATA
    CTGCAGTACA TGTGCAAGCT GTCGGATCAG AGCCTCCGAT CTGCCGCAAC CCGCGCGATG
    GCCGATTTTG TGTGGTCCTC TATGCGAGAC CCACTGGATG TTGCTGTCAA CTTCGACACC
    GAAGGATTGG CCCTGGCCTT TAAGTACTTC ACATCGACCA CGCTAACCAT GCGACTGGCA
    GGCATGGCCC AGATCAACGC ACACATTAAT CTATTTAACG AGATCTGCAC CACCGAAACG
    GTCAACGAAG TGGAGCTGTT CGGGCAGCGC ATCGCGAACT GGCTGACTGA AAATCATATT
    GTACAGCACC TGTTTGGCCC GAATCTGCAC GTAGAAATCG TTAAACAGGC ACACGTGCTG
    CTCAACTTTT TGGCGGTAGA GAACCAGATC TCTGAAGAGG ACATCAAGCT GATCTGGCAA
    GCCACCCAGC TGAAGCACTG CTCAAAGACG ATCTTCGACA TCCTGCCTTC GCTGGTGAAG
    AATCTGACCC CGCGCCCGGC AATGCACCTT TACTCCCTCC TTTGCCGCAT GGATCCCAAG
    GAGCACACTG AGCAGAGCAT ATACATAGCC TCAGCGCTAA CAAAACAGCT GTGGACTCGT
    GATACCTCAC GCAGTCAGAT GAACCTGATG CAAGACCACC TGCTGGGGTC CAACGTCACC
    GCTTCCAGCT CTGACAGCGG CAGCATTGAG GGCAGCAACA CAGAGGACGA CCACGTGGGC
    GCCGACGACA GTAGCATTGC CAGCGGTGGC GGCGGCGGAG GTGTTGGTAA CAAGAGTCCA
    ATCGATGGAG TTACTCCATG CAAGCAGGCC CGGCACAGGC GGCACATCTG TGACCCCACC
    ACCGAGAAGG GAAAGCAAAT AAGTCCCGAG GATATGGCCA AAGTAGACTT GGTGAACAAA
    CGGATTGTGA ACATTATCGA CAACACAAGC AGTGAAGAGG AGCTGCAATC GCGGGCCGCA
    CTGGAGCTGC AACTGCATCG CAGCCGCAAA AAGACGAGCA ACAAGCGACG GCGACAAAAA
    ACCAACAAAC AGATCATTCT GCCCCATGAG CTGGTCGAGA TTTGGGACGG AGTTGAAGAT
    GTGCCCAGCA GTGACGAGGC TGATGGCGAA GCCGATGGTG ATGGCGAGGG CGAACTTTTA
    GCGGACAGCG ATGAGTGCAG CGATGGTGCT ACGAGTCAGC TGGTACCCGA CGCCGTGCTA
    AAGCATCTGC AGGGAGAGGG ACCTTTTATC AGGGCCATCG AGACCAATAT CAATGAGCTG
    CTCAGTGGTG CCGAGAACGA TGGCAGTTAC TCATCCCCGA TGAGTAACAA ATCTGAAAAG
    AACCTGGCTG ACTTCGATGA CGAGGACGTG TCGCCTTGCG AGGAGGAGCT GGCCCAGCTG
    GTCAGCTCCC GCGCCAATTG TTCGGACGTT CCACCAGCCT TTGCAGCCGC TGCCGCCGCC
    ATGATGGTGG CCCAGTCGGC GATGGCCCTT CAGAAGTCTG GAGAGTCGAA TGTGGCTGCT
    GCTGCCGCTG CTGTGGCCGC TGCTGCAGCG GCTACCGGAA CTGCACAGCA AACGCTGGTG
    GCAATGAACC GGCAGGCCAG CGTTGCTGCA GCTGCCGCCG TGGTTGCTGC GGCCAAGGCA
    AAATCGGACT CAGATGTCGA TCTGATGGAT GTGGTTTCCG GCGGAAAGCA GCACCATTCA
    CCAAAGGCGA GCCAGGGCAG CTCTACGTCC GGGAGTACAC CGGTGCAGCC CTCGTTCAAG
    CTAAATGATG TCTGCCAGCC CGGAAACACA CTGCTTTGGG ATCTGCTTCA AGACGATAAA
    ATTGGCCAAC TAGGCGAGTC TCTGGCTCTA GAGGCGGAGA AAGCTCTGGC CACTCTCCTG
    TGCTTCAGCA TGGACCGCCA ACTGCGAACG AAATTCATCG AGGGTTGTCT GTATAACGTC
    GCCAACAACC GGAGTGTTAT TGTCAGTCTG CGACTGCTGC CCAAACTGTT CGCCTCGTTC
    CAACAGTTTC GACCCTCGGA CACGCACTCT ATGACGATGT GGGCAGAGCG AAATCACCGG
    ATGATGCAGT GCTTTTTCAA TAATATACGT CACTATGCGC GTCGCCATGC CGAGGTACTG
    ATCACCCAAA ACGGAGAGCA GCAGCAGCAG CAGCTGGGCG GACAACTCTA CTCGCACAAG
    ACACAAGTTT CTGTAAGACT GCAGTTCCTA TCATCGATAT TCTCGACGGT GGGATCCCCG
    AAAAGCTTCC GCCTAACGCT GGAGCAGCTT GACGCGCTAT GGGAGTGGCT GGCCCACGAT
    CCGGAGTGTG CCGACTGCTA CTTTTCGTGG CTGCAGGCCC AGGCCAAAGG CGGTGATCAG
    CATGCTCTGG GCATTGAAGC ACTTCAGCAT TTGTATTTGA AGAAACTCCC AGAGCTGCGA
    CCCGAGGAGT TCTCTATGGT TGCGTTGGGA CTATTTCAAC AGCTCTGTAG CTTCGCCCGC
    ATCGCCATGG CCGAGTACGA CAATCATAGC GACCAGATCT CGGCGAGCGC CAGTGCCGTT
    GGCATGTACC ATCTTTGGAA GATAGCGTTG CGTGCCCAGA GCAACGATGT CTCCTTGGCG
    GCCATACAGT ACATCAACAT GTACTACATG GGTCAACAAT TGCGCCTGGA GAAGGAGTTC
    GTTTCACAGT GTATGGAAAA CCTAGTTCAA GCGGCCACGG CTTTGGAAAG CATCGATGAT
    GAGAATGCTT TGATGCGTGT CCAGCGTGGC CTGCTCTTGC TGAACACCCA TTTGGATACG
    TTCCGGCGTC GATATGCGTT TCATCTGCGC CGCTGGGCCA TCGAAGGCAA AGGGATTGGA
    TCGCACAGTA ACCTTAAAAA TGAGGGAGCG GGTCCACCAC TACGAATAGT GCTGCAACAG
    GCAGGACTTT CGGAAAAAAG CCTTTTGCAG ATGCACGCGT GTGACTTAAT CGCCGACCTC
    AAGGCTGAGG TGTCCAAGTG GTGGGAGAGC CTTCAAACTG GACTAGCTGC TCCTGTGCTC
    GGTCTGCTGC TCAGCGATGG ACCGCTACGC ATTATCACAC AGGGACAGGA ATTGACCTCG
    GATTACGATG AACGGAGTCT CGGAGATGCT GGTTTCAAGG ATAACCAGAT CGTCTACGTA
    TCGTTAGGTG GACGTGGAGC CAGGCGAAAG GAATCCAATT TGGAGCATCC GTCAATGCTG
    CCGCCCCCAC CAAAGGAGTG TTTGCCCACG GTGCTCCTGC TGCAGCCAAA GTACTTCGAG
    AAGCTATTTT GCCTGATGCA GACCCTGGGC GATATGCAAC CGCAAGCGTC GACTGTAAAT
    CCCCAGCACC ACACAAAGGC GCAATTGCTC TCGCGACGCG TATGGGATAT CCTCGCCATG
    CTGCCAACTA ATCCGCACAT TCTAGACGCC TTTAAGAGTC TGGTTACGGA CCTAAGCGAA
    CTCGAGCAGC TTGATGCTGG CGGAGAGGAG GAGCAACTGG CCACCAAGCG CAAGCAAATC
    AAGCAGAAGT TCCGAGATCT TCTCGACCCG AACAACTTGC AAAAGTTCAT GTACTCCCTC
    CATATTGTGG AGAGTCTGGC CCTAACAAGT TCCAGACGTG GTGAATCCAA CGGCAATGTG
    GCCATGGGAA ATACACCGGA ACAGGTACGG GTGAAGAAAT CCTCGATGGG AAGACGGAGA
    AATAGCAACG AGCAGCCGCC ACCGCCGCCG CCAGAAGTCA AGATGAGCAA GGAGCAACTG
    TGCGAGCTGG AGGCCCCACT GACGCCCACA CCCTCGACTG GCTTGCAGGA TGTGGAAACG
    GAGGCCAGCA GTAGCAGCGG TGGCGACAAA GAAAACCAAC CGAAACAACA CAGCAAGCGT
    CAGAAGAAAG GCGAAACTTT TGAACAGGAA AAGGAGCGCC CAGTGGGTTG CTCCACGCCG
    CCGTCTCCAA CTCCTCCGCC ACCAGCACTT TCGGTAGTGG AAAGAGGTGA CAATAAGTGG
    AGTGAGGCGT TCGTCAAATG TGGTGGCCTT CGTCATCTGT ACGAAATCTT TAGTGAGGGC
    CAACTACAAC AAAGCGCACA TCCCAAGGAG TTGGCTTTAA ATGAATGGCG ACACGATTGC
    CTGGCCAGCC TGCTGCGCAT TCTCTGGCTT CTTGGATTCG AGGAACTGCA GTCGGCGGAT
    GCCCATGTTC TAATGTCACG TCCGCACCCG TTTATGCTAC AGTTGATGGA GGTACCACAG
    TGCCTGACCC GACTCTCATC GATCCTTAAC GACGAAGTTC ACCAGCAGCA TCAGGCTTCT
    TCGGCCAATC CACTGGTATT CCCCTATCAG TTCCAGCACT TGCGTACTGG CTTTTGGGGC
    AGGGCCCAGC TCATCCAGTT CGCTATGAAC ATTCTAGTGA GTTTTGTGCA CGCCTCAGCG
    GAAGCGAGAA GGCTTCTCTG GGCCCCAACT GGTACCGATC ACTGTCGATG GCTGCAGAAG
    TTCATCCTGG AGGATCCCGA ACCGGCGGTA CGGCGTGAGA TATGCGCTGG TCTTTATCGC
    ATTTGCTTAG GAAACGCACA TAGCTATCGC CTCTTACTAG CACCACTGCT GCACAAGCTC
    ATCGCCCTGT TGCCATTGGC CGAGCAGATG AGCTCTGGCA ACCAGCACAC GCAGTTTTTG
    CTCTCGGAAG AGGGCAAGGA TCCCTACGGA CCCGCCTGCC GTGACTACTT TTGGCTGCTG
    GCCAGATTGG TGGATACTCT CTCCCCGGAA ATGGTGGCCG AGGAGCACAT CGACATTGAA
    ATGCTGTGCG AAAGCATCTC GCAGAGCATT CTCACCCGAG AGTATTATGA ACTGCGCCAT
    GGTTACCAGG ACGATGGCTT GGTGGGTCTC CTAAATCTCA TGTCCAATTT GATCAAGTAC
    GACACCACAT TCAAGTACAC ACCCAAGGCG TTATCCTTTA TTGAGCAACT GATTGGCTTC
    CTGTTTGACA TGCCCTCGCC GGCAGATCGC CAGAAGCCCA AGTGCAAGTC GGCGTCGTCT
    CGAGCATCTG CCTACGATCT GCTGGTTGAA CTTTGCCGTG GATGCGCCAC AAACTATGCA
    TATCTGCATG GACGTCTGTT GGCACAGCAC AAATCCGGAC CCAAGCAGCC GTATCCCTGG
    GACTATTGGC CGCGCGATGA AGGCAGAGCT GAGTGCGGAT ATGTGGGGCT AACTAATTTG
    GGAGCCACTT GCTATATGGC CTCTTGCGTC CAGCACTTGT ACATGATGCC ACAGGCGCGA
    GCAGCTGTTT TGCGAGTTCC ACCCAACGCC GCCCGTAAGC ATGGGCCCAC ACTTCTGGAA
    CTACAACGGA TGTTTGCCTA CCTACTGGAG TCGGAACGGA AGTCTTACAA CCCGCGAAGC
    TTCTGCCGGG TGTACCAGAT GGATCACCAG CCGCTAAATA CGGGAGAGCA AAAGGATATG
    GCAGAGTTCT TTATCGATTT GGTGTCCAAA CTGGAAGATA TGACGCCTGA CTTGAAGCAC
    CTGGTGAAAA GGCTCTTCTG TGGATCCTTG AGCAATAATG TCGTCTCCCT GGATTGCGGT
    CACGTTTCCC GCACGGCCGA GGACTTTTAC ACGGTTCGCT GCCAGGTTGC AGATATGCGC
    AATCTGCAGG AATCCCTGGA CGAAGTCACC GTTAAGGATA CCCTCGAGGG CGACAACATG
    TACACCTGCT CGCAGTGCGG CAAAAAAGTA CGAGCCGAGA AGCGGGCTTG CTTCAAGAAG
    CTTCCCCAGA TCTTGTGCTT CAACACCATG CGCTACACCT TCAATATGGT CACCATGCTC
    AAGGAGAAGG TCAACACGCA CTTTTCCTTT CCGCTACGCC TGAACATGTG TCACTATGTG
    GAGAAGACCT TGATGCCGCA GCAGTACAAA GAGGAGCGAG AAAGGCGCCA AAAGGAGAAG
    GAAGGAGCAG ATGGTAGTGG CGATGGGAAT GACAACGAAA AGGCAGAGGC CACGCTAGAT
    GACGATATTG AAGAATGCTA CGAGTACGAA CTGGTGGGCG TCACTGTCCA CACGGGCACA
    GCGGATGGCG GCCACTACTA CAGCTTTATA AAGGAGCGGA CGAAGACTAG TTACCATACG
    CACGAGCGGT GGTTTCTTTT CAACGATGCC GAAGTGAAGC CTTTCGATCC CTCACAGATA
    GCGGCGGAGT GCTTTGGCGG CGAGATGACG AGCAAAACCT ACGACTCAGT GACGGAGAAA
    TATCTGGACT TTAGTTTCGA GAAGACCAAC TCTGCCTACA TGCTGTTCTA CGAGCGCCGA
    CTGCCAGAGC ATCTGCAGCG CCGGCATTCC GAGCTGCTGG TTACACCCAC GCCCAGTCCC
    ACAGTGGAAG AGAAGTCCGA GGCGGAGGAG CCCACTAAAA TGGAGACAAG CTCTAGCGAA
    ATCAAGGCAG ATGTGGATGT GGAAGTGGAA GTTGAGGAGA AGGACAAGGA GAAGCCTGCG
    CAGACTGACA CAGAATCTAA GGAAACGCCT GCAAAAGAGG AGATCGCGGA TGACAAATCG
    AAGCAGGATG AGCCTGAGGA AAAGAAAATC GAAAAGCAAT CCAGAGAGGG CGAAGAGAAA
    TCGGAAACGG ACGAAAAGCC CACGGAGATG ACGACCGTGA GCACTGAGGA GGAAAAGCAG
    CCAACAGCCA ATTGTGATAA TCACCAGCAA AACAACAATA GCAACAGCAA AGCCAGCAAC
    GATCAGCAAC CATCCACGTC GAAGGCTGCC CAGAAGCTAC AACTATTTCG TCCATTGTTG
    AACAAGGAGC TCGAGGATTG GATTTGGCAG GACAATCGAC AGTTCCTGCA GGATCGCAAC
    ATTTTCGAGC ACACATATTT CAACTTTATG TGGCAGATAT GTGGGCACAT ACCCCAATCA
    TTAATATCAG AGACGGACGT CACCTGCATG GCAGCCAAGT TGTCTGTGTC CTTTTTCATT
    GAGACTTTTA TTCATGCTAA GGAAAAGCCC ACTATGGTGC CCTGGGTGGA GCTGCTGACC
    AAGCAGTTCA ATGCCAGCCA AGAGGCCTGC GAGTGGTTTC TGTCGCACAT GTCGCAGGAG
    CCCTACTGGC CGGTCCAGGT GCTAATCCAG TGTCCCAACC AAATGGTGCG TCAAATGTTT
    CAGCGTTTGG TAATCCATGT CATCCAGCAA CTACGTGCCT CACATGCTCA TCTGTATCTG
    GAAGTGGAGA CAGACGAGGA TGACAAGGAG TTAATAGGCC AAGCCTCTTG CGTCACCCGT
    TTCATTGGTT CGCTCATCTC GCTCCTGGAG CACGGTGCCC GAGCGAACCT GCGGCACTTG
    TCCGAGTACT TTGGACTGTT GTGCGAGTTC TCACGGATGG GAGATGAGGA AGCCATGTAC
    TTGCTGCGCA TCGGTGTACT CAAGAGCCTG GTCGACTTCT ATCTGGGTCA CAAGCAAACA
    GACAGCATAG ACATAAGCTC TGACAACGAA GACAACTCCT CTGAAGAGGC GCTGTCCGTG
    GAGAAGATGC GTCCGGCCTC GCTGGACAAG ATGATTGCTC TGTGCGCCAG CCTAGTGGAA
    AGATCAAGAG GAGCCGACTT CCGGCTGCGA CTCTCGCCCA AAGACTTCTC TGCAATCGCC
    GGCGGCAAGG GTTTTCCATT TTTGTACCAA CAAATAAAGG ACGGCATTAA TCCGCACCAG
    ACAAAGCATT TAATACACGC GCTTTGTCGC TGGGATGAGC GCCTGGCCAC GCAGATCATT
    GGCATGCTCT TCGCGTCGGT AACCAAGCAC ACGGAGCTGT GCGCGCCATT CTTCAAGCTG
    CTAACTCTGC TCACCGAGAC GCAGGGCGGT CCAGTGGGCC TGCCTTGTTT CACCCAGTTG
    ATCCTGCCAC GAATGTGGGA CGCCGCCGAG TACTGTCCAC AGTCGGTGCT CGACTGGCTC
    TCGCTGCAGG CCACCAAGAA CAAGATCGCC CACGCCTGGA TACTTCAATC GGCCGAGAAG
    TGGCTCGAGC AATTCCTGCT GGCCCACGAT AACACTCGTG TTCGGAACGC TGCCGCCTTT
    CTGCTGGTGG CACTGGTACC CTCCCAGCCA TTCCGGGCCA ACTTCCGCGC CCACTCGCAA
    CACAAGCTGC TGGCCCTCAA TCCGCACAGT TATCGCGACA TAAATAGCGA TGCCCAGGCG
    GTGCTGCACC AGGTGATCAC GCTGCTCCTG CGATTGCTGC GTCCAGCGCG CGTCTATGCG
    GATATCGGAG CCCATGGCAC GACCAAGCTG ACCGCGTACT TTAATCTGCT CAGCTATTGT
    ATGGTTTCAA AGACTGAAAA ACTAATGGCA AGTTCGTACA TGCGCTCCCT GTGGGAGCTG
    TTCCATCCAC GCCTCTCGGA GCCCAGTGTT CCGGCCCATC ACAATAAGCA CGCACTATTA
    ACCTTCTGGC ACCACTCACT GGTGGACTGT CCGGAGAATG CTGCCCAGGT GGCCAACTGC
    CCGGAGATTA CGCGCAACAT TGCATTTAAC TATATACTGG CCGATCATGA TGACGCGGAG
    ATAGTCACCT ACAACCGTTC CATGCTGCCC GCCTATTATG GACTACTTCG TCTGTGCTGC
    GAGCAGAGCC GTGCACTCAC ACGCCAATTG TCCCAGCATC AGAATCTTCA GTGGGCATTT
    AAAAACATCA CACCGCATCC CACGCAGTAC GCGGCCGCCG TGGACGAGCT TTTCAAGTTG
    ATGGCTCTGT TCGCCACCCG TCATCCGGAT GCTAGTGAAC AGGAAAAGCT TGATGTGACC
    CAGTTTAGGA GGGCTGTGAT TGTGTCCTAC ACCAGTAGTC TGGATGCACG TGTCTCTTGG
    TCCACGCTGA TCAGTGCTCT TAAGATACTC GTGGACAATG AGGAGGACCG CACGATGGTG
    ATCTTCAACG GCGGCATTGA GATGTGTTTC GAGGCGCTGC ACACGCTACA TTCCATGCAC
    CACGAGGCCA CCGCCTGCCA CGTGGCCGGA GACCTATTCG ATCTCTTGGG CGAGATGCTA
    CTCCTCCTAG CCACTCTTCG AACCCGCACA GACAGTCCTG CTCAAAAGAA GCAGCAGCAA
    CAACAGCAGC AGCTGGAGCA ACAGTTGCAG TTGCAGCAGC AACAGATGCT GCAGAAACAA
    CAGCAGCAGT CGCAAAGCCA AACGCAAACA CCGCAGTCGC CGCAGCAAAA GGAAAAGCAG
    CTGCAGCAAC AAATGCAGCA GCATCTGCAG CTGCAACAGT TGCAGCAAAT GCAGTTCCAG
    CAGCAACACT TCCTGCGCCA GCAACATCAA CACTCGGCGC TGGCAAAGGC ACTGCCGGAT
    GCAGTGAAGC GACTGGCTAC GCTACTCAAC ACCTTCAATT CGCCAGAGAT CAGTCGCATG
    GCCTTGGAGG TGCTCAAGGA GCTGGTGCGC AATCCCAGCC TGGAAACCAT TAGTATTCTG
    GCGCCCATTC TTATCAACTG TCATCTGTCC GTGGCGAATG CTCCCAATGC GATCGGTCCT
    CTGGGACCGT ACTTTCCGCG TCGCGGTGCG AAGCATACGC CTTGGCCATT GGGAGCCAAG
    AACTCACCCC GTCCACCGCG GCCCATGGTA CAGATGTGCA TCGCTCTGGC TGAGCTGACA
    CCACGCGGTC TCGATGCCGA TTACGACGTT CAGGTGGAGT CCTTTTACCG ACCCTACCAT
    GACTTTATCG ATGTCATGAT GCGCATGTGC GTCAACACGG GCACTCTGAA CGACACGCTA
    GTCAAGCTGC AATGCTTGGT GGCCATCGAG TCCACGCCGC TGCACTTCAC CTATTTCCCC
    AAATTCTGGG TGGGCATACA CAACAATGCT CTGACGCACA AATACGTTGA GCTGCTGGTG
    AAAAATCAAC TGTTGGTAGA GTACCTTCAC AATGTCCTTA GGGACGAGCG AAGCATGTTG
    AAGGATGCCT GCGTTCGTGA ATTTCTGGAG CTCTATTATC ACAAGGTTGC CGCCCAGCTG
    CCAGTGGCCC GTATGATCTA CACAATCAAC TATGGTATGC ACTCCAAGGA CGACATTGAT
    GAGCTGTGTG GCGATCTGTT CGCCATCCGA ATCATTGCCC AGGCCACTGG AGTGCCGGCC
    TCAGTGCGCA AGGAGTTGCG AGGATCGCTG AGGGCGCTTC AGAACAAGAG CGAGCGGTTT
    AGGAAGGAAT CGGAGAGGGA TCCATTTCCG AACAAGAAGC AGAAACGTGA CTCGCAAAAG
    ATCAAGGAAA AGGAGCACCC TCAGCCGGAG AGCGAGAAAG AGACCTCAAC GGAGAACGAC
    AAACCATCAG ATGTTTCCAT GGAATCCAGC GGTAACGCGG AGCAAGCAAC GGACAGCACA
    AAACCACCAA CTCCTGCAGG CAGCGACGAT GAGCAGATGG ACAAAACTTC AGTTCCCTCG
    TCCGATGACG AGACAGAGTT GGAGGACGAG CTGCAGCCGA CGCCCAAGCG CAACAAGAAG
    GCTTCGAATA AGAAGACCGC CCAGGATCGT GTCAACGAGG AGCGCGAGAA GCGACTTATC
    ACGCTGATCA CCATGGAATC ATATATCCAG AGCATTTTTG CCATCCTTAA GAGAGATGCC
    AGTGTCCCCA GTTCGGGAGC GACTAAGGAG CCCAGTGAGG AACCGTGTGG CGAGGCATCC
    ACTTCGGCGG CTGCCGCTGT AAAGTTATCT CGTCCCAAAG GAGCCTGTAC TCCGCCGGAG
    CCAATTACCG ACGTAAACGA TACGCGTTGC AACATAGATA CTGAGACAGA GGCGGAGGCG
    GATGAGCAAT CTCCAAAGAC GAGCCAGACA AATGGTAGCC AACAGAACGA ATCCCCGCCA
    GCGGCGACCA GTGCAGACAC TGCACCCGCG AATCCTTCAC CAGCTCCCGC GGCCGCCGTT
    GCGTCCACAT CCCAGGCGGC AAGTCCAACA CAGATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa05979
    Clone ID Related Accession (Same CDS sequence) NM_206564.2
    Accession Version NM_206564.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8499bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product puffyeye, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_206564.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGTGCGAGG TGTGCGCCGA CTTCCAGAAT CTCCTGGAGC TCTATGAGGT GCGCGTGGCC 
    AGCTCGGATC TGAAGTTCCA GCTGCTGCTG AAGAGCGAGA TCGAGACCAC CTTCAACTAC
    ATCCAGTCGT GGCCGCAGCG TCAGTGCATG TGCCTGTACC GGGACACCAA GAACTATGAC
    CGTTTCAACC TGGTAGTGCA GTCGCTCATC TGTCTGACGG TGCAGCATCT GAAGCACATC
    GACCACCTCA TCGACAACTA CAAGCGGCTA ACGGCCAGTG CTGCCCAGGT GGTGGCCCAG
    GCCCAGCAGC AGCGTGAACA GCAGCGGGAC GAAGCGTCAG CCCAGGCAGA GGCAAAGGAA
    TCATCCGCGC CAGCGGAAGA ACCTAAGAAG GAGGAGCCAT CTGGGTCCGC CGGTGAGGAG
    GCTCAGGGCT CCGGCGACGG GCCAGCCAAA AAGCCGCCAG TTGGCCCCTG CACTCCCCCT
    CCCCCGCAAA CGGCCAATCC TCAGCACAAA TCCCACCTGC AGTACACAGA GGAGCCCTGG
    ATCCTGCCCG AGGTGGAGAA ACTGTTGGTG CTCGTTTCAA AGGTGTTCCT GCTCAACTTC
    CCGCTGTACA TCGCCCACAA ACATGGCATG CACTCTCGGC TGGACGACCT GCAGGCTGAG
    GAGGCCCACC ACCTGGCCCT CATCTGCGAT CTTCACGACA ACGACCTGCC CATCTATCTG
    CTGCGGAATG TGAGCCTGTT CTGCAACTCG GGCGGCTTTG GCGCCATGTC GCTGTGCTTC
    GAACATCCCG ATCTGCCAGT GTCCACCGCT CACTCGATGA CCGCTGCCGT CTCAAATGTG
    AAACTTTGGC TGAACTATCA CTGTAATACT CAGCTCTTCG TGCCGCTGCG AAGTCGGATA
    CTGCAGTACA TGTGCAAGCT GTCGGATCAG AGCCTCCGAT CTGCCGCAAC CCGCGCGATG
    GCCGATTTTG TGTGGTCCTC TATGCGAGAC CCACTGGATG TTGCTGTCAA CTTCGACACC
    GAAGGATTGG CCCTGGCCTT TAAGTACTTC ACATCGACCA CGCTAACCAT GCGACTGGCA
    GGCATGGCCC AGATCAACGC ACACATTAAT CTATTTAACG AGATCTGCAC CACCGAAACG
    GTCAACGAAG TGGAGCTGTT CGGGCAGCGC ATCGCGAACT GGCTGACTGA AAATCATATT
    GTACAGCACC TGTTTGGCCC GAATCTGCAC GTAGAAATCG TTAAACAGGC ACACGTGCTG
    CTCAACTTTT TGGCGGTAGA GAACCAGATC TCTGAAGAGG ACATCAAGCT GATCTGGCAA
    GCCACCCAGC TGAAGCACTG CTCAAAGACG ATCTTCGACA TCCTGCCTTC GCTGGTGAAG
    AATCTGACCC CGCGCCCGGC AATGCACCTT TACTCCCTCC TTTGCCGCAT GGATCCCAAG
    GAGCACACTG AGCAGAGCAT ATACATAGCC TCAGCGCTAA CAAAACAGCT GTGGACTCGT
    GATACCTCAC GCAGTCAGAT GAACCTGATG CAAGACCACC TGCTGGGGTC CAACGTCACC
    GCTTCCAGCT CTGACAGCGG CAGCATTGAG GGCAGCAACA CAGAGGACGA CCACGTGGGC
    GCCGACGACA GTAGCATTGC CAGCGGTGGC GGCGGCGGAG GTGTTGGTAA CAAGAGTCCA
    ATCGATGGAG TTACTCCATG CAAGCAGGCC CGGCACAGGC GGCACATCTG TGACCCCACC
    ACCGAGAAGG GAAAGCAAAT AAGTCCCGAG GATATGGCCA AAGTAGACTT GGTGAACAAA
    CGGATTGTGA ACATTATCGA CAACACAAGC AGTGAAGAGG AGCTGCAATC GCGGGCCGCA
    CTGGAGCTGC AACTGCATCG CAGCCGCAAA AAGACGAGCA ACAAGCGACG GCGACAAAAA
    ACCAACAAAC AGATCATTCT GCCCCATGAG CTGGTCGAGA TTTGGGACGG AGTTGAAGAT
    GTGCCCAGCA GTGACGAGGC TGATGGCGAA GCCGATGGTG ATGGCGAGGG CGAACTTTTA
    GCGGACAGCG ATGAGTGCAG CGATGGTGCT ACGAGTCAGC TGGTACCCGA CGCCGTGCTA
    AAGCATCTGC AGGGAGAGGG ACCTTTTATC AGGGCCATCG AGACCAATAT CAATGAGCTG
    CTCAGTGGTG CCGAGAACGA TGGCAGTTAC TCATCCCCGA TGAGTAACAA ATCTGAAAAG
    AACCTGGCTG ACTTCGATGA CGAGGACGTG TCGCCTTGCG AGGAGGAGCT GGCCCAGCTG
    GTCAGCTCCC GCGCCAATTG TTCGGACGTT CCACCAGCCT TTGCAGCCGC TGCCGCCGCC
    ATGATGGTGG CCCAGTCGGC GATGGCCCTT CAGAAGTCTG GAGAGTCGAA TGTGGCTGCT
    GCTGCCGCTG CTGTGGCCGC TGCTGCAGCG GCTACCGGAA CTGCACAGCA AACGCTGGTG
    GCAATGAACC GGCAGGCCAG CGTTGCTGCA GCTGCCGCCG TGGTTGCTGC GGCCAAGGCA
    AAATCGGACT CAGATGTCGA TCTGATGGAT GTGGTTTCCG GCGGAAAGCA GCACCATTCA
    CCAAAGGCGA GCCAGGGCAG CTCTACGTCC GGGAGTACAC CGGTGCAGCC CTCGTTCAAG
    CTAAATGATG TCTGCCAGCC CGGAAACACA CTGCTTTGGG ATCTGCTTCA AGACGATAAA
    ATTGGCCAAC TAGGCGAGTC TCTGGCTCTA GAGGCGGAGA AAGCTCTGGC CACTCTCCTG
    TGCTTCAGCA TGGACCGCCA ACTGCGAACG AAATTCATCG AGGGTTGTCT GTATAACGTC
    GCCAACAACC GGAGTGTTAT TGTCAGTCTG CGACTGCTGC CCAAACTGTT CGCCTCGTTC
    CAACAGTTTC GACCCTCGGA CACGCACTCT ATGACGATGT GGGCAGAGCG AAATCACCGG
    ATGATGCAGT GCTTTTTCAA TAATATACGT CACTATGCGC GTCGCCATGC CGAGGTACTG
    ATCACCCAAA ACGGAGAGCA GCAGCAGCAG CAGCTGGGCG GACAACTCTA CTCGCACAAG
    ACACAAGTTT CTGTAAGACT GCAGTTCCTA TCATCGATAT TCTCGACGGT GGGATCCCCG
    AAAAGCTTCC GCCTAACGCT GGAGCAGCTT GACGCGCTAT GGGAGTGGCT GGCCCACGAT
    CCGGAGTGTG CCGACTGCTA CTTTTCGTGG CTGCAGGCCC AGGCCAAAGG CGGTGATCAG
    CATGCTCTGG GCATTGAAGC ACTTCAGCAT TTGTATTTGA AGAAACTCCC AGAGCTGCGA
    CCCGAGGAGT TCTCTATGGT TGCGTTGGGA CTATTTCAAC AGCTCTGTAG CTTCGCCCGC
    ATCGCCATGG CCGAGTACGA CAATCATAGC GACCAGATCT CGGCGAGCGC CAGTGCCGTT
    GGCATGTACC ATCTTTGGAA GATAGCGTTG CGTGCCCAGA GCAACGATGT CTCCTTGGCG
    GCCATACAGT ACATCAACAT GTACTACATG GGTCAACAAT TGCGCCTGGA GAAGGAGTTC
    GTTTCACAGT GTATGGAAAA CCTAGTTCAA GCGGCCACGG CTTTGGAAAG CATCGATGAT
    GAGAATGCTT TGATGCGTGT CCAGCGTGGC CTGCTCTTGC TGAACACCCA TTTGGATACG
    TTCCGGCGTC GATATGCGTT TCATCTGCGC CGCTGGGCCA TCGAAGGCAA AGGGATTGGA
    TCGCACAGTA ACCTTAAAAA TGAGGGAGCG GGTCCACCAC TACGAATAGT GCTGCAACAG
    GCAGGACTTT CGGAAAAAAG CCTTTTGCAG ATGCACGCGT GTGACTTAAT CGCCGACCTC
    AAGGCTGAGG TGTCCAAGTG GTGGGAGAGC CTTCAAACTG GACTAGCTGC TCCTGTGCTC
    GGTCTGCTGC TCAGCGATGG ACCGCTACGC ATTATCACAC AGGGACAGGA ATTGACCTCG
    GATTACGATG AACGGAGTCT CGGAGATGCT GGTTTCAAGG ATAACCAGAT CGTCTACGTA
    TCGTTAGGTG GACGTGGAGC CAGGCGAAAG GAATCCAATT TGGAGCATCC GTCAATGCTG
    CCGCCCCCAC CAAAGGAGTG TTTGCCCACG GTGCTCCTGC TGCAGCCAAA GTACTTCGAG
    AAGCTATTTT GCCTGATGCA GACCCTGGGC GATATGCAAC CGCAAGCGTC GACTGTAAAT
    CCCCAGCACC ACACAAAGGC GCAATTGCTC TCGCGACGCG TATGGGATAT CCTCGCCATG
    CTGCCAACTA ATCCGCACAT TCTAGACGCC TTTAAGAGTC TGGTTACGGA CCTAAGCGAA
    CTCGAGCAGC TTGATGCTGG CGGAGAGGAG GAGCAACTGG CCACCAAGCG CAAGCAAATC
    AAGCAGAAGT TCCGAGATCT TCTCGACCCG AACAACTTGC AAAAGTTCAT GTACTCCCTC
    CATATTGTGG AGAGTCTGGC CCTAACAAGT TCCAGACGTG GTGAATCCAA CGGCAATGTG
    GCCATGGGAA ATACACCGGA ACAGGTACGG GTGAAGAAAT CCTCGATGGG AAGACGGAGA
    AATAGCAACG AGCAGCCGCC ACCGCCGCCG CCAGAAGTCA AGATGAGCAA GGAGCAACTG
    TGCGAGCTGG AGGCCCCACT GACGCCCACA CCCTCGACTG GCTTGCAGGA TGTGGAAACG
    GAGGCCAGCA GTAGCAGCGG TGGCGACAAA GAAAACCAAC CGAAACAACA CAGCAAGCGT
    CAGAAGAAAG GCGAAACTTT TGAACAGGAA AAGGAGCGCC CAGTGGGTTG CTCCACGCCG
    CCGTCTCCAA CTCCTCCGCC ACCAGCACTT TCGGTAGTGG AAAGAGGTGA CAATAAGTGG
    AGTGAGGCGT TCGTCAAATG TGGTGGCCTT CGTCATCTGT ACGAAATCTT TAGTGAGGGC
    CAACTACAAC AAAGCGCACA TCCCAAGGAG TTGGCTTTAA ATGAATGGCG ACACGATTGC
    CTGGCCAGCC TGCTGCGCAT TCTCTGGCTT CTTGGATTCG AGGAACTGCA GTCGGCGGAT
    GCCCATGTTC TAATGTCACG TCCGCACCCG TTTATGCTAC AGTTGATGGA GGTACCACAG
    TGCCTGACCC GACTCTCATC GATCCTTAAC GACGAAGTTC ACCAGCAGCA TCAGGCTTCT
    TCGGCCAATC CACTGGTATT CCCCTATCAG TTCCAGCACT TGCGTACTGG CTTTTGGGGC
    AGGGCCCAGC TCATCCAGTT CGCTATGAAC ATTCTAGTGA GTTTTGTGCA CGCCTCAGCG
    GAAGCGAGAA GGCTTCTCTG GGCCCCAACT GGTACCGATC ACTGTCGATG GCTGCAGAAG
    TTCATCCTGG AGGATCCCGA ACCGGCGGTA CGGCGTGAGA TATGCGCTGG TCTTTATCGC
    ATTTGCTTAG GAAACGCACA TAGCTATCGC CTCTTACTAG CACCACTGCT GCACAAGCTC
    ATCGCCCTGT TGCCATTGGC CGAGCAGATG AGCTCTGGCA ACCAGCACAC GCAGTTTTTG
    CTCTCGGAAG AGGGCAAGGA TCCCTACGGA CCCGCCTGCC GTGACTACTT TTGGCTGCTG
    GCCAGATTGG TGGATACTCT CTCCCCGGAA ATGGTGGCCG AGGAGCACAT CGACATTGAA
    ATGCTGTGCG AAAGCATCTC GCAGAGCATT CTCACCCGAG AGTATTATGA ACTGCGCCAT
    GGTTACCAGG ACGATGGCTT GGTGGGTCTC CTAAATCTCA TGTCCAATTT GATCAAGTAC
    GACACCACAT TCAAGTACAC ACCCAAGGCG TTATCCTTTA TTGAGCAACT GATTGGCTTC
    CTGTTTGACA TGCCCTCGCC GGCAGATCGC CAGAAGCCCA AGTGCAAGTC GGCGTCGTCT
    CGAGCATCTG CCTACGATCT GCTGGTTGAA CTTTGCCGTG GATGCGCCAC AAACTATGCA
    TATCTGCATG GACGTCTGTT GGCACAGCAC AAATCCGGAC CCAAGCAGCC GTATCCCTGG
    GACTATTGGC CGCGCGATGA AGGCAGAGCT GAGTGCGGAT ATGTGGGGCT AACTAATTTG
    GGAGCCACTT GCTATATGGC CTCTTGCGTC CAGCACTTGT ACATGATGCC ACAGGCGCGA
    GCAGCTGTTT TGCGAGTTCC ACCCAACGCC GCCCGTAAGC ATGGGCCCAC ACTTCTGGAA
    CTACAACGGA TGTTTGCCTA CCTACTGGAG TCGGAACGGA AGTCTTACAA CCCGCGAAGC
    TTCTGCCGGG TGTACCAGAT GGATCACCAG CCGCTAAATA CGGGAGAGCA AAAGGATATG
    GCAGAGTTCT TTATCGATTT GGTGTCCAAA CTGGAAGATA TGACGCCTGA CTTGAAGCAC
    CTGGTGAAAA GGCTCTTCTG TGGATCCTTG AGCAATAATG TCGTCTCCCT GGATTGCGGT
    CACGTTTCCC GCACGGCCGA GGACTTTTAC ACGGTTCGCT GCCAGGTTGC AGATATGCGC
    AATCTGCAGG AATCCCTGGA CGAAGTCACC GTTAAGGATA CCCTCGAGGG CGACAACATG
    TACACCTGCT CGCAGTGCGG CAAAAAAGTA CGAGCCGAGA AGCGGGCTTG CTTCAAGAAG
    CTTCCCCAGA TCTTGTGCTT CAACACCATG CGCTACACCT TCAATATGGT CACCATGCTC
    AAGGAGAAGG TCAACACGCA CTTTTCCTTT CCGCTACGCC TGAACATGTG TCACTATGTG
    GAGAAGACCT TGATGCCGCA GCAGTACAAA GAGGAGCGAG AAAGGCGCCA AAAGGAGAAG
    GAAGGAGCAG ATGGTAGTGG CGATGGGAAT GACAACGAAA AGGCAGAGGC CACGCTAGAT
    GACGATATTG AAGAATGCTA CGAGTACGAA CTGGTGGGCG TCACTGTCCA CACGGGCACA
    GCGGATGGCG GCCACTACTA CAGCTTTATA AAGGAGCGGA CGAAGACTAG TTACCATACG
    CACGAGCGGT GGTTTCTTTT CAACGATGCC GAAGTGAAGC CTTTCGATCC CTCACAGATA
    GCGGCGGAGT GCTTTGGCGG CGAGATGACG AGCAAAACCT ACGACTCAGT GACGGAGAAA
    TATCTGGACT TTAGTTTCGA GAAGACCAAC TCTGCCTACA TGCTGTTCTA CGAGCGCCGA
    CTGCCAGAGC ATCTGCAGCG CCGGCATTCC GAGCTGCTGG TTACACCCAC GCCCAGTCCC
    ACAGTGGAAG AGAAGTCCGA GGCGGAGGAG CCCACTAAAA TGGAGACAAG CTCTAGCGAA
    ATCAAGGCAG ATGTGGATGT GGAAGTGGAA GTTGAGGAGA AGGACAAGGA GAAGCCTGCG
    CAGACTGACA CAGAATCTAA GGAAACGCCT GCAAAAGAGG AGATCGCGGA TGACAAATCG
    AAGCAGGATG AGCCTGAGGA AAAGAAAATC GAAAAGCAAT CCAGAGAGGG CGAAGAGAAA
    TCGGAAACGG ACGAAAAGCC CACGGAGATG ACGACCGTGA GCACTGAGGA GGAAAAGCAG
    CCAACAGCCA ATTGTGATAA TCACCAGCAA AACAACAATA GCAACAGCAA AGCCAGCAAC
    GATCAGCAAC CATCCACGTC GAAGGCTGCC CAGAAGCTAC AACTATTTCG TCCATTGTTG
    AACAAGGAGC TCGAGGATTG GATTTGGCAG GACAATCGAC AGTTCCTGCA GGATCGCAAC
    ATTTTCGAGC ACACATATTT CAACTTTATG TGGCAGATAT GTGGGCACAT ACCCCAATCA
    TTAATATCAG AGACGGACGT CACCTGCATG GCAGCCAAGT TGTCTGTGTC CTTTTTCATT
    GAGACTTTTA TTCATGCTAA GGAAAAGCCC ACTATGGTGC CCTGGGTGGA GCTGCTGACC
    AAGCAGTTCA ATGCCAGCCA AGAGGCCTGC GAGTGGTTTC TGTCGCACAT GTCGCAGGAG
    CCCTACTGGC CGGTCCAGGT GCTAATCCAG TGTCCCAACC AAATGGTGCG TCAAATGTTT
    CAGCGTTTGG TAATCCATGT CATCCAGCAA CTACGTGCCT CACATGCTCA TCTGTATCTG
    GAAGTGGAGA CAGACGAGGA TGACAAGGAG TTAATAGGCC AAGCCTCTTG CGTCACCCGT
    TTCATTGGTT CGCTCATCTC GCTCCTGGAG CACGGTGCCC GAGCGAACCT GCGGCACTTG
    TCCGAGTACT TTGGACTGTT GTGCGAGTTC TCACGGATGG GAGATGAGGA AGCCATGTAC
    TTGCTGCGCA TCGGTGTACT CAAGAGCCTG GTCGACTTCT ATCTGGGTCA CAAGCAAACA
    GACAGCATAG ACATAAGCTC TGACAACGAA GACAACTCCT CTGAAGAGGC GCTGTCCGTG
    GAGAAGATGC GTCCGGCCTC GCTGGACAAG ATGATTGCTC TGTGCGCCAG CCTAGTGGAA
    AGATCAAGAG GAGCCGACTT CCGGCTGCGA CTCTCGCCCA AAGACTTCTC TGCAATCGCC
    GGCGGCAAGG TTATACTCAA AAAGCTGGAA AGCTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_996287.1
    CDS285..8783
    Translation

    Target ORF information:

    RefSeq Version NM_206564.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster puffyeye (puf), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_206564.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGTGCGAGG TGTGCGCCGA CTTCCAGAAT CTCCTGGAGC TCTATGAGGT GCGCGTGGCC 
    AGCTCGGATC TGAAGTTCCA GCTGCTGCTG AAGAGCGAGA TCGAGACCAC CTTCAACTAC
    ATCCAGTCGT GGCCGCAGCG TCAGTGCATG TGCCTGTACC GGGACACCAA GAACTATGAC
    CGTTTCAACC TGGTAGTGCA GTCGCTCATC TGTCTGACGG TGCAGCATCT GAAGCACATC
    GACCACCTCA TCGACAACTA CAAGCGGCTA ACGGCCAGTG CTGCCCAGGT GGTGGCCCAG
    GCCCAGCAGC AGCGTGAACA GCAGCGGGAC GAAGCGTCAG CCCAGGCAGA GGCAAAGGAA
    TCATCCGCGC CAGCGGAAGA ACCTAAGAAG GAGGAGCCAT CTGGGTCCGC CGGTGAGGAG
    GCTCAGGGCT CCGGCGACGG GCCAGCCAAA AAGCCGCCAG TTGGCCCCTG CACTCCCCCT
    CCCCCGCAAA CGGCCAATCC TCAGCACAAA TCCCACCTGC AGTACACAGA GGAGCCCTGG
    ATCCTGCCCG AGGTGGAGAA ACTGTTGGTG CTCGTTTCAA AGGTGTTCCT GCTCAACTTC
    CCGCTGTACA TCGCCCACAA ACATGGCATG CACTCTCGGC TGGACGACCT GCAGGCTGAG
    GAGGCCCACC ACCTGGCCCT CATCTGCGAT CTTCACGACA ACGACCTGCC CATCTATCTG
    CTGCGGAATG TGAGCCTGTT CTGCAACTCG GGCGGCTTTG GCGCCATGTC GCTGTGCTTC
    GAACATCCCG ATCTGCCAGT GTCCACCGCT CACTCGATGA CCGCTGCCGT CTCAAATGTG
    AAACTTTGGC TGAACTATCA CTGTAATACT CAGCTCTTCG TGCCGCTGCG AAGTCGGATA
    CTGCAGTACA TGTGCAAGCT GTCGGATCAG AGCCTCCGAT CTGCCGCAAC CCGCGCGATG
    GCCGATTTTG TGTGGTCCTC TATGCGAGAC CCACTGGATG TTGCTGTCAA CTTCGACACC
    GAAGGATTGG CCCTGGCCTT TAAGTACTTC ACATCGACCA CGCTAACCAT GCGACTGGCA
    GGCATGGCCC AGATCAACGC ACACATTAAT CTATTTAACG AGATCTGCAC CACCGAAACG
    GTCAACGAAG TGGAGCTGTT CGGGCAGCGC ATCGCGAACT GGCTGACTGA AAATCATATT
    GTACAGCACC TGTTTGGCCC GAATCTGCAC GTAGAAATCG TTAAACAGGC ACACGTGCTG
    CTCAACTTTT TGGCGGTAGA GAACCAGATC TCTGAAGAGG ACATCAAGCT GATCTGGCAA
    GCCACCCAGC TGAAGCACTG CTCAAAGACG ATCTTCGACA TCCTGCCTTC GCTGGTGAAG
    AATCTGACCC CGCGCCCGGC AATGCACCTT TACTCCCTCC TTTGCCGCAT GGATCCCAAG
    GAGCACACTG AGCAGAGCAT ATACATAGCC TCAGCGCTAA CAAAACAGCT GTGGACTCGT
    GATACCTCAC GCAGTCAGAT GAACCTGATG CAAGACCACC TGCTGGGGTC CAACGTCACC
    GCTTCCAGCT CTGACAGCGG CAGCATTGAG GGCAGCAACA CAGAGGACGA CCACGTGGGC
    GCCGACGACA GTAGCATTGC CAGCGGTGGC GGCGGCGGAG GTGTTGGTAA CAAGAGTCCA
    ATCGATGGAG TTACTCCATG CAAGCAGGCC CGGCACAGGC GGCACATCTG TGACCCCACC
    ACCGAGAAGG GAAAGCAAAT AAGTCCCGAG GATATGGCCA AAGTAGACTT GGTGAACAAA
    CGGATTGTGA ACATTATCGA CAACACAAGC AGTGAAGAGG AGCTGCAATC GCGGGCCGCA
    CTGGAGCTGC AACTGCATCG CAGCCGCAAA AAGACGAGCA ACAAGCGACG GCGACAAAAA
    ACCAACAAAC AGATCATTCT GCCCCATGAG CTGGTCGAGA TTTGGGACGG AGTTGAAGAT
    GTGCCCAGCA GTGACGAGGC TGATGGCGAA GCCGATGGTG ATGGCGAGGG CGAACTTTTA
    GCGGACAGCG ATGAGTGCAG CGATGGTGCT ACGAGTCAGC TGGTACCCGA CGCCGTGCTA
    AAGCATCTGC AGGGAGAGGG ACCTTTTATC AGGGCCATCG AGACCAATAT CAATGAGCTG
    CTCAGTGGTG CCGAGAACGA TGGCAGTTAC TCATCCCCGA TGAGTAACAA ATCTGAAAAG
    AACCTGGCTG ACTTCGATGA CGAGGACGTG TCGCCTTGCG AGGAGGAGCT GGCCCAGCTG
    GTCAGCTCCC GCGCCAATTG TTCGGACGTT CCACCAGCCT TTGCAGCCGC TGCCGCCGCC
    ATGATGGTGG CCCAGTCGGC GATGGCCCTT CAGAAGTCTG GAGAGTCGAA TGTGGCTGCT
    GCTGCCGCTG CTGTGGCCGC TGCTGCAGCG GCTACCGGAA CTGCACAGCA AACGCTGGTG
    GCAATGAACC GGCAGGCCAG CGTTGCTGCA GCTGCCGCCG TGGTTGCTGC GGCCAAGGCA
    AAATCGGACT CAGATGTCGA TCTGATGGAT GTGGTTTCCG GCGGAAAGCA GCACCATTCA
    CCAAAGGCGA GCCAGGGCAG CTCTACGTCC GGGAGTACAC CGGTGCAGCC CTCGTTCAAG
    CTAAATGATG TCTGCCAGCC CGGAAACACA CTGCTTTGGG ATCTGCTTCA AGACGATAAA
    ATTGGCCAAC TAGGCGAGTC TCTGGCTCTA GAGGCGGAGA AAGCTCTGGC CACTCTCCTG
    TGCTTCAGCA TGGACCGCCA ACTGCGAACG AAATTCATCG AGGGTTGTCT GTATAACGTC
    GCCAACAACC GGAGTGTTAT TGTCAGTCTG CGACTGCTGC CCAAACTGTT CGCCTCGTTC
    CAACAGTTTC GACCCTCGGA CACGCACTCT ATGACGATGT GGGCAGAGCG AAATCACCGG
    ATGATGCAGT GCTTTTTCAA TAATATACGT CACTATGCGC GTCGCCATGC CGAGGTACTG
    ATCACCCAAA ACGGAGAGCA GCAGCAGCAG CAGCTGGGCG GACAACTCTA CTCGCACAAG
    ACACAAGTTT CTGTAAGACT GCAGTTCCTA TCATCGATAT TCTCGACGGT GGGATCCCCG
    AAAAGCTTCC GCCTAACGCT GGAGCAGCTT GACGCGCTAT GGGAGTGGCT GGCCCACGAT
    CCGGAGTGTG CCGACTGCTA CTTTTCGTGG CTGCAGGCCC AGGCCAAAGG CGGTGATCAG
    CATGCTCTGG GCATTGAAGC ACTTCAGCAT TTGTATTTGA AGAAACTCCC AGAGCTGCGA
    CCCGAGGAGT TCTCTATGGT TGCGTTGGGA CTATTTCAAC AGCTCTGTAG CTTCGCCCGC
    ATCGCCATGG CCGAGTACGA CAATCATAGC GACCAGATCT CGGCGAGCGC CAGTGCCGTT
    GGCATGTACC ATCTTTGGAA GATAGCGTTG CGTGCCCAGA GCAACGATGT CTCCTTGGCG
    GCCATACAGT ACATCAACAT GTACTACATG GGTCAACAAT TGCGCCTGGA GAAGGAGTTC
    GTTTCACAGT GTATGGAAAA CCTAGTTCAA GCGGCCACGG CTTTGGAAAG CATCGATGAT
    GAGAATGCTT TGATGCGTGT CCAGCGTGGC CTGCTCTTGC TGAACACCCA TTTGGATACG
    TTCCGGCGTC GATATGCGTT TCATCTGCGC CGCTGGGCCA TCGAAGGCAA AGGGATTGGA
    TCGCACAGTA ACCTTAAAAA TGAGGGAGCG GGTCCACCAC TACGAATAGT GCTGCAACAG
    GCAGGACTTT CGGAAAAAAG CCTTTTGCAG ATGCACGCGT GTGACTTAAT CGCCGACCTC
    AAGGCTGAGG TGTCCAAGTG GTGGGAGAGC CTTCAAACTG GACTAGCTGC TCCTGTGCTC
    GGTCTGCTGC TCAGCGATGG ACCGCTACGC ATTATCACAC AGGGACAGGA ATTGACCTCG
    GATTACGATG AACGGAGTCT CGGAGATGCT GGTTTCAAGG ATAACCAGAT CGTCTACGTA
    TCGTTAGGTG GACGTGGAGC CAGGCGAAAG GAATCCAATT TGGAGCATCC GTCAATGCTG
    CCGCCCCCAC CAAAGGAGTG TTTGCCCACG GTGCTCCTGC TGCAGCCAAA GTACTTCGAG
    AAGCTATTTT GCCTGATGCA GACCCTGGGC GATATGCAAC CGCAAGCGTC GACTGTAAAT
    CCCCAGCACC ACACAAAGGC GCAATTGCTC TCGCGACGCG TATGGGATAT CCTCGCCATG
    CTGCCAACTA ATCCGCACAT TCTAGACGCC TTTAAGAGTC TGGTTACGGA CCTAAGCGAA
    CTCGAGCAGC TTGATGCTGG CGGAGAGGAG GAGCAACTGG CCACCAAGCG CAAGCAAATC
    AAGCAGAAGT TCCGAGATCT TCTCGACCCG AACAACTTGC AAAAGTTCAT GTACTCCCTC
    CATATTGTGG AGAGTCTGGC CCTAACAAGT TCCAGACGTG GTGAATCCAA CGGCAATGTG
    GCCATGGGAA ATACACCGGA ACAGGTACGG GTGAAGAAAT CCTCGATGGG AAGACGGAGA
    AATAGCAACG AGCAGCCGCC ACCGCCGCCG CCAGAAGTCA AGATGAGCAA GGAGCAACTG
    TGCGAGCTGG AGGCCCCACT GACGCCCACA CCCTCGACTG GCTTGCAGGA TGTGGAAACG
    GAGGCCAGCA GTAGCAGCGG TGGCGACAAA GAAAACCAAC CGAAACAACA CAGCAAGCGT
    CAGAAGAAAG GCGAAACTTT TGAACAGGAA AAGGAGCGCC CAGTGGGTTG CTCCACGCCG
    CCGTCTCCAA CTCCTCCGCC ACCAGCACTT TCGGTAGTGG AAAGAGGTGA CAATAAGTGG
    AGTGAGGCGT TCGTCAAATG TGGTGGCCTT CGTCATCTGT ACGAAATCTT TAGTGAGGGC
    CAACTACAAC AAAGCGCACA TCCCAAGGAG TTGGCTTTAA ATGAATGGCG ACACGATTGC
    CTGGCCAGCC TGCTGCGCAT TCTCTGGCTT CTTGGATTCG AGGAACTGCA GTCGGCGGAT
    GCCCATGTTC TAATGTCACG TCCGCACCCG TTTATGCTAC AGTTGATGGA GGTACCACAG
    TGCCTGACCC GACTCTCATC GATCCTTAAC GACGAAGTTC ACCAGCAGCA TCAGGCTTCT
    TCGGCCAATC CACTGGTATT CCCCTATCAG TTCCAGCACT TGCGTACTGG CTTTTGGGGC
    AGGGCCCAGC TCATCCAGTT CGCTATGAAC ATTCTAGTGA GTTTTGTGCA CGCCTCAGCG
    GAAGCGAGAA GGCTTCTCTG GGCCCCAACT GGTACCGATC ACTGTCGATG GCTGCAGAAG
    TTCATCCTGG AGGATCCCGA ACCGGCGGTA CGGCGTGAGA TATGCGCTGG TCTTTATCGC
    ATTTGCTTAG GAAACGCACA TAGCTATCGC CTCTTACTAG CACCACTGCT GCACAAGCTC
    ATCGCCCTGT TGCCATTGGC CGAGCAGATG AGCTCTGGCA ACCAGCACAC GCAGTTTTTG
    CTCTCGGAAG AGGGCAAGGA TCCCTACGGA CCCGCCTGCC GTGACTACTT TTGGCTGCTG
    GCCAGATTGG TGGATACTCT CTCCCCGGAA ATGGTGGCCG AGGAGCACAT CGACATTGAA
    ATGCTGTGCG AAAGCATCTC GCAGAGCATT CTCACCCGAG AGTATTATGA ACTGCGCCAT
    GGTTACCAGG ACGATGGCTT GGTGGGTCTC CTAAATCTCA TGTCCAATTT GATCAAGTAC
    GACACCACAT TCAAGTACAC ACCCAAGGCG TTATCCTTTA TTGAGCAACT GATTGGCTTC
    CTGTTTGACA TGCCCTCGCC GGCAGATCGC CAGAAGCCCA AGTGCAAGTC GGCGTCGTCT
    CGAGCATCTG CCTACGATCT GCTGGTTGAA CTTTGCCGTG GATGCGCCAC AAACTATGCA
    TATCTGCATG GACGTCTGTT GGCACAGCAC AAATCCGGAC CCAAGCAGCC GTATCCCTGG
    GACTATTGGC CGCGCGATGA AGGCAGAGCT GAGTGCGGAT ATGTGGGGCT AACTAATTTG
    GGAGCCACTT GCTATATGGC CTCTTGCGTC CAGCACTTGT ACATGATGCC ACAGGCGCGA
    GCAGCTGTTT TGCGAGTTCC ACCCAACGCC GCCCGTAAGC ATGGGCCCAC ACTTCTGGAA
    CTACAACGGA TGTTTGCCTA CCTACTGGAG TCGGAACGGA AGTCTTACAA CCCGCGAAGC
    TTCTGCCGGG TGTACCAGAT GGATCACCAG CCGCTAAATA CGGGAGAGCA AAAGGATATG
    GCAGAGTTCT TTATCGATTT GGTGTCCAAA CTGGAAGATA TGACGCCTGA CTTGAAGCAC
    CTGGTGAAAA GGCTCTTCTG TGGATCCTTG AGCAATAATG TCGTCTCCCT GGATTGCGGT
    CACGTTTCCC GCACGGCCGA GGACTTTTAC ACGGTTCGCT GCCAGGTTGC AGATATGCGC
    AATCTGCAGG AATCCCTGGA CGAAGTCACC GTTAAGGATA CCCTCGAGGG CGACAACATG
    TACACCTGCT CGCAGTGCGG CAAAAAAGTA CGAGCCGAGA AGCGGGCTTG CTTCAAGAAG
    CTTCCCCAGA TCTTGTGCTT CAACACCATG CGCTACACCT TCAATATGGT CACCATGCTC
    AAGGAGAAGG TCAACACGCA CTTTTCCTTT CCGCTACGCC TGAACATGTG TCACTATGTG
    GAGAAGACCT TGATGCCGCA GCAGTACAAA GAGGAGCGAG AAAGGCGCCA AAAGGAGAAG
    GAAGGAGCAG ATGGTAGTGG CGATGGGAAT GACAACGAAA AGGCAGAGGC CACGCTAGAT
    GACGATATTG AAGAATGCTA CGAGTACGAA CTGGTGGGCG TCACTGTCCA CACGGGCACA
    GCGGATGGCG GCCACTACTA CAGCTTTATA AAGGAGCGGA CGAAGACTAG TTACCATACG
    CACGAGCGGT GGTTTCTTTT CAACGATGCC GAAGTGAAGC CTTTCGATCC CTCACAGATA
    GCGGCGGAGT GCTTTGGCGG CGAGATGACG AGCAAAACCT ACGACTCAGT GACGGAGAAA
    TATCTGGACT TTAGTTTCGA GAAGACCAAC TCTGCCTACA TGCTGTTCTA CGAGCGCCGA
    CTGCCAGAGC ATCTGCAGCG CCGGCATTCC GAGCTGCTGG TTACACCCAC GCCCAGTCCC
    ACAGTGGAAG AGAAGTCCGA GGCGGAGGAG CCCACTAAAA TGGAGACAAG CTCTAGCGAA
    ATCAAGGCAG ATGTGGATGT GGAAGTGGAA GTTGAGGAGA AGGACAAGGA GAAGCCTGCG
    CAGACTGACA CAGAATCTAA GGAAACGCCT GCAAAAGAGG AGATCGCGGA TGACAAATCG
    AAGCAGGATG AGCCTGAGGA AAAGAAAATC GAAAAGCAAT CCAGAGAGGG CGAAGAGAAA
    TCGGAAACGG ACGAAAAGCC CACGGAGATG ACGACCGTGA GCACTGAGGA GGAAAAGCAG
    CCAACAGCCA ATTGTGATAA TCACCAGCAA AACAACAATA GCAACAGCAA AGCCAGCAAC
    GATCAGCAAC CATCCACGTC GAAGGCTGCC CAGAAGCTAC AACTATTTCG TCCATTGTTG
    AACAAGGAGC TCGAGGATTG GATTTGGCAG GACAATCGAC AGTTCCTGCA GGATCGCAAC
    ATTTTCGAGC ACACATATTT CAACTTTATG TGGCAGATAT GTGGGCACAT ACCCCAATCA
    TTAATATCAG AGACGGACGT CACCTGCATG GCAGCCAAGT TGTCTGTGTC CTTTTTCATT
    GAGACTTTTA TTCATGCTAA GGAAAAGCCC ACTATGGTGC CCTGGGTGGA GCTGCTGACC
    AAGCAGTTCA ATGCCAGCCA AGAGGCCTGC GAGTGGTTTC TGTCGCACAT GTCGCAGGAG
    CCCTACTGGC CGGTCCAGGT GCTAATCCAG TGTCCCAACC AAATGGTGCG TCAAATGTTT
    CAGCGTTTGG TAATCCATGT CATCCAGCAA CTACGTGCCT CACATGCTCA TCTGTATCTG
    GAAGTGGAGA CAGACGAGGA TGACAAGGAG TTAATAGGCC AAGCCTCTTG CGTCACCCGT
    TTCATTGGTT CGCTCATCTC GCTCCTGGAG CACGGTGCCC GAGCGAACCT GCGGCACTTG
    TCCGAGTACT TTGGACTGTT GTGCGAGTTC TCACGGATGG GAGATGAGGA AGCCATGTAC
    TTGCTGCGCA TCGGTGTACT CAAGAGCCTG GTCGACTTCT ATCTGGGTCA CAAGCAAACA
    GACAGCATAG ACATAAGCTC TGACAACGAA GACAACTCCT CTGAAGAGGC GCTGTCCGTG
    GAGAAGATGC GTCCGGCCTC GCTGGACAAG ATGATTGCTC TGTGCGCCAG CCTAGTGGAA
    AGATCAAGAG GAGCCGACTT CCGGCTGCGA CTCTCGCCCA AAGACTTCTC TGCAATCGCC
    GGCGGCAAGG TTATACTCAA AAAGCTGGAA AGCTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa07738
    Clone ID Related Accession (Same CDS sequence) NM_001275994.2
    Accession Version NM_001275994.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11793bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product puffyeye, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001275994.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    ATGTGCGAGG TGTGCGCCGA CTTCCAGAAT CTCCTGGAGC TCTATGAGGT GCGCGTGGCC 
    AGCTCGGATC TGAAGTTCCA GCTGCTGCTG AAGAGCGAGA TCGAGACCAC CTTCAACTAC
    ATCCAGTCGT GGCCGCAGCG TCAGTGCATG TGCCTGTACC GGGACACCAA GAACTATGAC
    CGTTTCAACC TGGTAGTGCA GTCGCTCATC TGTCTGACGG TGCAGCATCT GAAGCACATC
    GACCACCTCA TCGACAACTA CAAGCGGCTA ACGGCCAGTG CTGCCCAGGT GGTGGCCCAG
    GCCCAGCAGC AGCGTGAACA GCAGCGGGAC GAAGCGTCAG CCCAGGCAGA GGCAAAGGAA
    TCATCCGCGC CAGCGGAAGA ACCTAAGAAG GAGGAGCCAT CTGGGTCCGC CGGTGAGGAG
    GCTCAGGGCT CCGGCGACGG GCCAGCCAAA AAGCCGCCAG TTGGCCCCTG CACTCCCCCT
    CCCCCGCAAA CGGCCAATCC TCAGCACAAA TCCCACCTGC AGTACACAGA GGAGCCCTGG
    ATCCTGCCCG AGGTGGAGAA ACTGTTGGTG CTCGTTTCAA AGGTGTTCCT GCTCAACTTC
    CCGCTGTACA TCGCCCACAA ACATGGCATG CACTCTCGGC TGGACGACCT GCAGGCTGAG
    GAGGCCCACC ACCTGGCCCT CATCTGCGAT CTTCACGACA ACGACCTGCC CATCTATCTG
    CTGCGGAATG TGAGCCTGTT CTGCAACTCG GGCGGCTTTG GCGCCATGTC GCTGTGCTTC
    GAACATCCCG ATCTGCCAGT GTCCACCGCT CACTCGATGA CCGCTGCCGT CTCAAATGTG
    AAACTTTGGC TGAACTATCA CTGTAATACT CAGCTCTTCG TGCCGCTGCG AAGTCGGATA
    CTGCAGTACA TGTGCAAGCT GTCGGATCAG AGCCTCCGAT CTGCCGCAAC CCGCGCGATG
    GCCGATTTTG TGTGGTCCTC TATGCGAGAC CCACTGGATG TTGCTGTCAA CTTCGACACC
    GAAGGATTGG CCCTGGCCTT TAAGTACTTC ACATCGACCA CGCTAACCAT GCGACTGGCA
    GGCATGGCCC AGATCAACGC ACACATTAAT CTATTTAACG AGATCTGCAC CACCGAAACG
    GTCAACGAAG TGGAGCTGTT CGGGCAGCGC ATCGCGAACT GGCTGACTGA AAATCATATT
    GTACAGCACC TGTTTGGCCC GAATCTGCAC GTAGAAATCG TTAAACAGGC ACACGTGCTG
    CTCAACTTTT TGGCGGTAGA GAACCAGATC TCTGAAGAGG ACATCAAGCT GATCTGGCAA
    GCCACCCAGC TGAAGCACTG CTCAAAGACG ATCTTCGACA TCCTGCCTTC GCTGGTGAAG
    AATCTGACCC CGCGCCCGGC AATGCACCTT TACTCCCTCC TTTGCCGCAT GGATCCCAAG
    GAGCACACTG AGCAGAGCAT ATACATAGCC TCAGCGCTAA CAAAACAGCT GTGGACTCGT
    GATACCTCAC GCAGTCAGAT GAACCTGATG CAAGACCACC TGCTGGGGTC CAACGTCACC
    GCTTCCAGCT CTGACAGCGG CAGCATTGAG GGCAGCAACA CAGAGGACGA CCACGTGGGC
    GCCGACGACA GTAGCATTGC CAGCGGTGGC GGCGGCGGAG GTGTTGGTAA CAAGAGTCCA
    ATCGATGGAG TTACTCCATG CAAGCAGGCC CGGCACAGGC GGCACATCTG TGACCCCACC
    ACCGAGAAGG GAAAGCAAAT AAGTCCCGAG GATATGGCCA AAGTAGACTT GGTGAACAAA
    CGGATTGTGA ACATTATCGA CAACACAAGC AGTGAAGAGG AGCTGCAATC GCGGGCCGCA
    CTGGAGCTGC AACTGCATCG CAGCCGCAAA AAGACGAGCA ACAAGCGACG GCGACAAAAA
    ACCAACAAAC AGATCATTCT GCCCCATGAG CTGGTCGAGA TTTGGGACGG AGTTGAAGAT
    GTGCCCAGCA GTGACGAGGC TGATGGCGAA GCCGATGGTG ATGGCGAGGG CGAACTTTTA
    GCGGACAGCG ATGAGTGCAG CGATGGTGCT ACGAGTCAGC TGGTACCCGA CGCCGTGCTA
    AAGCATCTGC AGGGAGAGGG ACCTTTTATC AGGGCCATCG AGACCAATAT CAATGAGCTG
    CTCAGTGGTG CCGAGAACGA TGGCAGTTAC TCATCCCCGA TGAGTAACAA ATCTGAAAAG
    AACCTGGCTG ACTTCGATGA CGAGGACGTG TCGCCTTGCG AGGAGGAGCT GGCCCAGCTG
    GTCAGCTCCC GCGCCAATTG TTCGGACGTT CCACCAGCCT TTGCAGCCGC TGCCGCCGCC
    ATGATGGTGG CCCAGTCGGC GATGGCCCTT CAGAAGTCTG GAGAGTCGAA TGTGGCTGCT
    GCTGCCGCTG CTGTGGCCGC TGCTGCAGCG GCTACCGGAA CTGCACAGCA AACGCTGGTG
    GCAATGAACC GGCAGGCCAG CGTTGCTGCA GCTGCCGCCG TGGTTGCTGC GGCCAAGGCA
    AAATCGGACT CAGATGTCGA TCTGATGGAT GTGGTTTCCG GCGGAAAGCA GCACCATTCA
    CCAAAGGCGA GCCAGGGCAG CTCTACGTCC GGGAGTACAC CGGTGCAGCC CTCGTTCAAG
    CTAAATGATG TCTGCCAGCC CGGAAACACA CTGCTTTGGG ATCTGCTTCA AGACGATAAA
    ATTGGCCAAC TAGGCGAGTC TCTGGCTCTA GAGGCGGAGA AAGCTCTGGC CACTCTCCTG
    TGCTTCAGCA TGGACCGCCA ACTGCGAACG AAATTCATCG AGGGTTGTCT GTATAACGTC
    GCCAACAACC GGAGTGTTAT TGTCAGTCTG CGACTGCTGC CCAAACTGTT CGCCTCGTTC
    CAACAGTTTC GACCCTCGGA CACGCACTCT ATGACGATGT GGGCAGAGCG AAATCACCGG
    ATGATGCAGT GCTTTTTCAA TAATATACGT CACTATGCGC GTCGCCATGC CGAGGTACTG
    ATCACCCAAA ACGGAGAGCA GCAGCAGCAG CAGCTGGGCG GACAACTCTA CTCGCACAAG
    ACACAAGTTT CTGTAAGACT GCAGTTCCTA TCATCGATAT TCTCGACGGT GGGATCCCCG
    AAAAGCTTCC GCCTAACGCT GGAGCAGCTT GACGCGCTAT GGGAGTGGCT GGCCCACGAT
    CCGGAGTGTG CCGACTGCTA CTTTTCGTGG CTGCAGGCCC AGGCCAAAGG CGGTGATCAG
    CATGCTCTGG GCATTGAAGC ACTTCAGCAT TTGTATTTGA AGAAACTCCC AGAGCTGCGA
    CCCGAGGAGT TCTCTATGGT TGCGTTGGGA CTATTTCAAC AGCTCTGTAG CTTCGCCCGC
    ATCGCCATGG CCGAGTACGA CAATCATAGC GACCAGATCT CGGCGAGCGC CAGTGCCGTT
    GGCATGTACC ATCTTTGGAA GATAGCGTTG CGTGCCCAGA GCAACGATGT CTCCTTGGCG
    GCCATACAGT ACATCAACAT GTACTACATG GGTCAACAAT TGCGCCTGGA GAAGGAGTTC
    GTTTCACAGT GTATGGAAAA CCTAGTTCAA GCGGCCACGG CTTTGGAAAG CATCGATGAT
    GAGAATGCTT TGATGCGTGT CCAGCGTGGC CTGCTCTTGC TGAACACCCA TTTGGATACG
    TTCCGGCGTC GATATGCGTT TCATCTGCGC CGCTGGGCCA TCGAAGGCAA AGGGATTGGA
    TCGCACAGTA ACCTTAAAAA TGAGGGAGCG GGTCCACCAC TACGAATAGT GCTGCAACAG
    GCAGGACTTT CGGAAAAAAG CCTTTTGCAG ATGCACGCGT GTGACTTAAT CGCCGACCTC
    AAGGCTGAGG TGTCCAAGTG GTGGGAGAGC CTTCAAACTG GACTAGCTGC TCCTGTGCTC
    GGTCTGCTGC TCAGCGATGG ACCGCTACGC ATTATCACAC AGGGACAGGA ATTGACCTCG
    GATTACGATG AACGGAGTCT CGGAGATGCT GGTTTCAAGG ATAACCAGAT CGTCTACGTA
    TCGTTAGGTG GACGTGGAGC CAGGCGAAAG GAATCCAATT TGGAGCATCC GTCAATGCTG
    CCGCCCCCAC CAAAGGAGTG TTTGCCCACG GTGCTCCTGC TGCAGCCAAA GTACTTCGAG
    AAGCTATTTT GCCTGATGCA GACCCTGGGC GATATGCAAC CGCAAGCGTC GACTGTAAAT
    CCCCAGCACC ACACAAAGGC GCAATTGCTC TCGCGACGCG TATGGGATAT CCTCGCCATG
    CTGCCAACTA ATCCGCACAT TCTAGACGCC TTTAAGAGTC TGGTTACGGA CCTAAGCGAA
    CTCGAGCAGC TTGATGCTGG CGGAGAGGAG GAGCAACTGG CCACCAAGCG CAAGCAAATC
    AAGCAGAAGT TCCGAGATCT TCTCGACCCG AACAACTTGC AAAAGTTCAT GTACTCCCTC
    CATATTGTGG AGAGTCTGGC CCTAACAAGT TCCAGACGTG GTGAATCCAA CGGCAATGTG
    GCCATGGGAA ATACACCGGA ACAGGTACGG GTGAAGAAAT CCTCGATGGG AAGACGGAGA
    AATAGCAACG AGCAGCCGCC ACCGCCGCCG CCAGAAGTCA AGATGAGCAA GGAGCAACTG
    TGCGAGCTGG AGGCCCCACT GACGCCCACA CCCTCGACTG GCTTGCAGGA TGTGGAAACG
    GAGGCCAGCA GTAGCAGCGG TGGCGACAAA GAAAACCAAC CGAAACAACA CAGCAAGCGT
    CAGAAGAAAG GCGAAACTTT TGAACAGGAA AAGGAGCGCC CAGTGGGTTG CTCCACGCCG
    CCGTCTCCAA CTCCTCCGCC ACCAGCACTT TCGGTAGTGG AAAGAGGTGA CAATAAGTGG
    AGTGAGGCGT TCGTCAAATG TGGTGGCCTT CGTCATCTGT ACGAAATCTT TAGTGAGGGC
    CAACTACAAC AAAGCGCACA TCCCAAGGAG TTGGCTTTAA ATGAATGGCG ACACGATTGC
    CTGGCCAGCC TGCTGCGCAT TCTCTGGCTT CTTGGATTCG AGGAACTGCA GTCGGCGGAT
    GCCCATGTTC TAATGTCACG TCCGCACCCG TTTATGCTAC AGTTGATGGA GGTACCACAG
    TGCCTGACCC GACTCTCATC GATCCTTAAC GACGAAGTTC ACCAGCAGCA TCAGGCTTCT
    TCGGCCAATC CACTGGTATT CCCCTATCAG TTCCAGCACT TGCGTACTGG CTTTTGGGGC
    AGGGCCCAGC TCATCCAGTT CGCTATGAAC ATTCTAGTGA GTTTTGTGCA CGCCTCAGCG
    GAAGCGAGAA GGCTTCTCTG GGCCCCAACT GGTACCGATC ACTGTCGATG GCTGCAGAAG
    TTCATCCTGG AGGATCCCGA ACCGGCGGTA CGGCGTGAGA TATGCGCTGG TCTTTATCGC
    ATTTGCTTAG GAAACGCACA TAGCTATCGC CTCTTACTAG CACCACTGCT GCACAAGCTC
    ATCGCCCTGT TGCCATTGGC CGAGCAGATG AGCTCTGGCA ACCAGCACAC GCAGTTTTTG
    CTCTCGGAAG AGGGCAAGGA TCCCTACGGA CCCGCCTGCC GTGACTACTT TTGGCTGCTG
    GCCAGATTGG TGGATACTCT CTCCCCGGAA ATGGTGGCCG AGGAGCACAT CGACATTGAA
    ATGCTGTGCG AAAGCATCTC GCAGAGCATT CTCACCCGAG AGTATTATGA ACTGCGCCAT
    GGTTACCAGG ACGATGGCTT GGTGGGTCTC CTAAATCTCA TGTCCAATTT GATCAAGTAC
    GACACCACAT TCAAGTACAC ACCCAAGGCG TTATCCTTTA TTGAGCAACT GATTGGCTTC
    CTGTTTGACA TGCCCTCGCC GGCAGATCGC CAGAAGCCCA AGTGCAAGTC GGCGTCGTCT
    CGAGCATCTG CCTACGATCT GCTGGTTGAA CTTTGCCGTG GATGCGCCAC AAACTATGCA
    TATCTGCATG GACGTCTGTT GGCACAGCAC AAATCCGGAC CCAAGCAGCC GTATCCCTGG
    GACTATTGGC CGCGCGATGA AGGCAGAGCT GAGTGCGGAT ATGTGGGGCT AACTAATTTG
    GGAGCCACTT GCTATATGGC CTCTTGCGTC CAGCACTTGT ACATGATGCC ACAGGCGCGA
    GCAGCTGTTT TGCGAGTTCC ACCCAACGCC GCCCGTAAGC ATGGGCCCAC ACTTCTGGAA
    CTACAACGGA TGTTTGCCTA CCTACTGGAG TCGGAACGGA AGTCTTACAA CCCGCGAAGC
    TTCTGCCGGG TGTACCAGAT GGATCACCAG CCGCTAAATA CGGGAGAGCA AAAGGATATG
    GCAGAGTTCT TTATCGATTT GGTGTCCAAA CTGGAAGATA TGACGCCTGA CTTGAAGCAC
    CTGGTGAAAA GGCTCTTCTG TGGATCCTTG AGCAATAATG TCGTCTCCCT GGATTGCGGT
    CACGTTTCCC GCACGGCCGA GGACTTTTAC ACGGTTCGCT GCCAGGTTGC AGATATGCGC
    AATCTGCAGG AATCCCTGGA CGAAGTCACC GTTAAGGATA CCCTCGAGGG CGACAACATG
    TACACCTGCT CGCAGTGCGG CAAAAAAGTA CGAGCCGAGA AGCGGGCTTG CTTCAAGAAG
    CTTCCCCAGA TCTTGTGCTT CAACACCATG CGCTACACCT TCAATATGGT CACCATGCTC
    AAGGAGAAGG TCAACACGCA CTTTTCCTTT CCGCTACGCC TGAACATGTG TCACTATGTG
    GAGAAGACCT TGATGCCGCA GCAGTACAAA GAGGAGCGAG AAAGGCGCCA AAAGGAGAAG
    GAAGGAGCAG ATGGTAGTGG CGATGGGAAT GACAACGAAA AGGCAGAGGC CACGCTAGAT
    GACGATATTG AAGAATGCTA CGAGTACGAA CTGGTGGGCG TCACTGTCCA CACGGGCACA
    GCGGATGGCG GCCACTACTA CAGCTTTATA AAGGAGCGGA CGAAGACTAG TTACCATACG
    CACGAGCGGT GGTTTCTTTT CAACGATGCC GAAGTGAAGC CTTTCGATCC CTCACAGATA
    GCGGCGGAGT GCTTTGGCGG CGAGATGACG AGCAAAACCT ACGACTCAGT GACGGAGAAA
    TATCTGGACT TTAGTTTCGA GAAGACCAAC TCTGCCTACA TGCTGTTCTA CGAGCGCCGA
    CTGCCAGAGC ATCTGCAGCG CCGGCATTCC GAGCTGCTGG TTACACCCAC GCCCAGTCCC
    ACAGTGGAAG AGAAGTCCGA GGCGGAGGAG CCCACTAAAA TGGAGACAAG CTCTAGCGAA
    ATCAAGGCAG ATGTGGATGT GGAAGTGGAA GTTGAGGAGA AGGACAAGGA GAAGCCTGCG
    CAGACTGACA CAGAATCTAA GGAAACGCCT GCAAAAGAGG AGATCGCGGA TGACAAATCG
    AAGCAGGATG AGCCTGAGGA AAAGAAAATC GAAAAGCAAT CCAGAGAGGG CGAAGAGAAA
    TCGGAAACGG ACGAAAAGCC CACGGAGATG ACGACCGTGA GCACTGAGGA GGAAAAGCAG
    CCAACAGCCA ATTGTGATAA TCACCAGCAA AACAACAATA GCAACAGCAA AGCCAGCAAC
    GATCAGCAAC CATCCACGTC GAAGGCTGCC CAGAAGCTAC AACTATTTCG TCCATTGTTG
    AACAAGGAGC TCGAGGATTG GATTTGGCAG GACAATCGAC AGTTCCTGCA GGATCGCAAC
    ATTTTCGAGC ACACATATTT CAACTTTATG TGGCAGATAT GTGGGCACAT ACCCCAATCA
    TTAATATCAG AGACGGACGT CACCTGCATG GCAGCCAAGT TGTCTGTGTC CTTTTTCATT
    GAGACTTTTA TTCATGCTAA GGAAAAGCCC ACTATGGTGC CCTGGGTGGA GCTGCTGACC
    AAGCAGTTCA ATGCCAGCCA AGAGGCCTGC GAGTGGTTTC TGTCGCACAT GTCGCAGGAG
    CCCTACTGGC CGGTCCAGGT GCTAATCCAG TGTCCCAACC AAATGGTGCG TCAAATGTTT
    CAGCGTTTGG TAATCCATGT CATCCAGCAA CTACGTGCCT CACATGCTCA TCTGTATCTG
    GAAGTGGAGA CAGACGAGGA TGACAAGGAG TTAATAGGCC AAGCCTCTTG CGTCACCCGT
    TTCATTGGTT CGCTCATCTC GCTCCTGGAG CACGGTGCCC GAGCGAACCT GCGGCACTTG
    TCCGAGTACT TTGGACTGTT GTGCGAGTTC TCACGGATGG GAGATGAGGA AGCCATGTAC
    TTGCTGCGCA TCGGTGTACT CAAGAGCCTG GTCGACTTCT ATCTGGGTCA CAAGCAAACA
    GACAGCATAG ACATAAGCTC TGACAACGAA GACAACTCCT CTGAAGAGGC GCTGTCCGTG
    GAGAAGATGC GTCCGGCCTC GCTGGACAAG ATGATTGCTC TGTGCGCCAG CCTAGTGGAA
    AGATCAAGAG GAGCCGACTT CCGGCTGCGA CTCTCGCCCA AAGACTTCTC TGCAATCGCC
    GGCGGCAAGG GTTTTCCATT TTTGTACCAA CAAATAAAGG ACGGCATTAA TCCGCACCAG
    ACAAAGCATT TAATACACGC GCTTTGTCGC TGGGATGAGC GCCTGGCCAC GCAGATCATT
    GGCATGCTCT TCGCGTCGGT AACCAAGCAC ACGGAGCTGT GCGCGCCATT CTTCAAGCTG
    CTAACTCTGC TCACCGAGAC GCAGGGCGGT CCAGTGGGCC TGCCTTGTTT CACCCAGTTG
    ATCCTGCCAC GAATGTGGGA CGCCGCCGAG TACTGTCCAC AGTCGGTGCT CGACTGGCTC
    TCGCTGCAGG CCACCAAGAA CAAGATCGCC CACGCCTGGA TACTTCAATC GGCCGAGAAG
    TGGCTCGAGC AATTCCTGCT GGCCCACGAT AACACTCGTG TTCGGAACGC TGCCGCCTTT
    CTGCTGGTGG CACTGGTACC CTCCCAGCCA TTCCGGGCCA ACTTCCGCGC CCACTCGCAA
    CACAAGCTGC TGGCCCTCAA TCCGCACAGT TATCGCGACA TAAATAGCGA TGCCCAGGCG
    GTGCTGCACC AGGTGATCAC GCTGCTCCTG CGATTGCTGC GTCCAGCGCG CGTCTATGCG
    GATATCGGAG CCCATGGCAC GACCAAGCTG ACCGCGTACT TTAATCTGCT CAGCTATTGT
    ATGGTTTCAA AGACTGAAAA ACTAATGGCA AGTTCGTACA TGCGCTCCCT GTGGGAGCTG
    TTCCATCCAC GCCTCTCGGA GCCCAGTGTT CCGGCCCATC ACAATAAGCA CGCACTATTA
    ACCTTCTGGC ACCACTCACT GGTGGACTGT CCGGAGAATG CTGCCCAGGT GGCCAACTGC
    CCGGAGATTA CGCGCAACAT TGCATTTAAC TATATACTGG CCGATCATGA TGACGCGGAG
    ATAGTCACCT ACAACCGTTC CATGCTGCCC GCCTATTATG GACTACTTCG TCTGTGCTGC
    GAGCAGAGCC GTGCACTCAC ACGCCAATTG TCCCAGCATC AGAATCTTCA GTGGGCATTT
    AAAAACATCA CACCGCATCC CACGCAGTAC GCGGCCGCCG TGGACGAGCT TTTCAAGTTG
    ATGGCTCTGT TCGCCACCCG TCATCCGGAT GCTAGTGAAC AGGAAAAGCT TGATGTGACC
    CAGTTTAGGA GGGCTGTGAT TGTGTCCTAC ACCAGTAGTC TGGATGCACG TGTCTCTTGG
    TCCACGCTGA TCAGTGCTCT TAAGATACTC GTGGACAATG AGGAGGACCG CACGATGGTG
    ATCTTCAACG GCGGCATTGA GATGTGTTTC GAGGCGCTGC ACACGCTACA TTCCATGCAC
    CACGAGGCCA CCGCCTGCCA CGTGGCCGGA GACCTATTCG ATCTCTTGGG CGAGATGCTA
    CTCCTCCTAG CCACTCTTCG AACCCGCACA GACAGTCCTG CTCAAAAGAA GCAGCAGCAA
    CAACAGCAGC AGCTGGAGCA ACAGTTGCAG TTGCAGCAGC AACAGATGCT GCAGAAACAA
    CAGCAGCAGT CGCAAAGCCA AACGCAAACA CCGCAGTCGC CGCAGCAAAA GGAAAAGCAG
    CTGCAGCAAC AAATGCAGCA GCATCTGCAG CTGCAACAGT TGCAGCAAAT GCAGTTCCAG
    CAGCAACACT TCCTGCGCCA GCAACATCAA CACTCGGCGC TGGCAAAGGC ACTGCCGGAT
    GCAGTGAAGC GACTGGCTAC GCTACTCAAC ACCTTCAATT CGCCAGAGAT CAGTCGCATG
    GCCTTGGAGG TGCTCAAGGA GCTGGTGCGC AATCCCAGCC TGGAAACCAT TAGTATTCTG
    GCGCCCATTC TTATCAACTG TCATCTGTCC GTGGCGAATG CTCCCAATGC GATCGGTCCT
    CTGGGACCGT ACTTTCCGCG TCGCGGTGCG AAGCATACGC CTTGGCCATT GGGAGCCAAG
    AACTCACCCC GTCCACCGCG GCCCATGGTA CAGATGTGCA TCGCTCTGGC TGAGCTGACA
    CCACGCGGTC TCGATGCCGA TTACGACGTT CAGGTGGAGT CCTTTTACCG ACCCTACCAT
    GACTTTATCG ATGTCATGAT GCGCATGTGC GTCAACACGG GCACTCTGAA CGACACGCTA
    GTCAAGCTGC AATGCTTGGT GGCCATCGAG TCCACGCCGC TGCACTTCAC CTATTTCCCC
    AAATTCTGGG TGGGCATACA CAACAATGCT CTGACGCACA AATACGTTGA GCTGCTGGTG
    AAAAATCAAC TGTTGGTAGA GTACCTTCAC AATGTCCTTA GGGACGAGCG AAGCATGTTG
    AAGGATGCCT GCGTTCGTGA ATTTCTGGAG CTCTATTATC ACAAGGTTGC CGCCCAGCTG
    CCAGTGGCCC GTATGATCTA CACAATCAAC TATGGTATGC ACTCCAAGGA CGACATTGAT
    GAGCTGTGTG GCGATCTGTT CGCCATCCGA ATCATTGCCC AGGCCACTGG AGTGCCGGCC
    TCAGTGCGCA AGGAGTTGCG AGGATCGCTG AGGGCGCTTC AGAACAAGAG CGAGCGGTTT
    AGGAAGGAAT CGGAGAGGGA TCCATTTCCG AACAAGAAGC AGAAACGTGA CTCGCAAAAG
    ATCAAGGAAA AGGAGCACCC TCAGCCGGAG AGCGAGAAAG AGACCTCAAC GGAGAACGAC
    AAACCATCAG ATGTTTCCAT GGAATCCAGC GGTAACGCGG AGCAAGCAAC GGACAGCACA
    AAACCACCAA CTCCTGCAGG CAGCGACGAT GAGCAGATGG ACAAAACTTC AGTTCCCTCG
    TCCGATGACG AGACAGAGTT GGAGGACGAG CTGCAGCCGA CGCCCAAGCG CAACAAGAAG
    GCTTCGAATA AGAAGACCGC CCAGGATCGT GTCAACGAGG AGCGCGAGAA GCGACTTATC
    ACGCTGATCA CCATGGAATC ATATATCCAG AGCATTTTTG CCATCCTTAA GAGAGATGCC
    AGTGTCCCCA GTTCGGGAGC GACTAAGGAG CCCAGTGAGG AACCGTGTGG CGAGGCATCC
    ACTTCGGCGG CTGCCGCTGT AAAGTTATCT CGTCCCAAAG GAGCCTGTAC TCCGCCGGAG
    CCAATTACCG ACGTAAACGA TACGCGTTGC AACATAGATA CTGAGACAGA GGCGGAGGCG
    GATGAGCAAT CTCCAAAGAC GAGCCAGACA AATGGTAGCC AACAGAACGA ATCCCCGCCA
    GCGGCGACCA GTGCAGACAC TGCACCCGCG AATCCTTCAC CAGCTCCCGC GGCCGCCGTT
    GCGTCCACAT CCCAGGCGGC AAGTCCAACA CAGATTTAGT TGTCATTCAA CGATTTGCTC
    AGCATTTTGT TTTTCGGCAA GCCATTGGAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001262923.1
    CDS358..12150
    Translation

    Target ORF information:

    RefSeq Version NM_001275994.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster puffyeye (puf), transcript variant H, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001275994.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    ATGTGCGAGG TGTGCGCCGA CTTCCAGAAT CTCCTGGAGC TCTATGAGGT GCGCGTGGCC 
    AGCTCGGATC TGAAGTTCCA GCTGCTGCTG AAGAGCGAGA TCGAGACCAC CTTCAACTAC
    ATCCAGTCGT GGCCGCAGCG TCAGTGCATG TGCCTGTACC GGGACACCAA GAACTATGAC
    CGTTTCAACC TGGTAGTGCA GTCGCTCATC TGTCTGACGG TGCAGCATCT GAAGCACATC
    GACCACCTCA TCGACAACTA CAAGCGGCTA ACGGCCAGTG CTGCCCAGGT GGTGGCCCAG
    GCCCAGCAGC AGCGTGAACA GCAGCGGGAC GAAGCGTCAG CCCAGGCAGA GGCAAAGGAA
    TCATCCGCGC CAGCGGAAGA ACCTAAGAAG GAGGAGCCAT CTGGGTCCGC CGGTGAGGAG
    GCTCAGGGCT CCGGCGACGG GCCAGCCAAA AAGCCGCCAG TTGGCCCCTG CACTCCCCCT
    CCCCCGCAAA CGGCCAATCC TCAGCACAAA TCCCACCTGC AGTACACAGA GGAGCCCTGG
    ATCCTGCCCG AGGTGGAGAA ACTGTTGGTG CTCGTTTCAA AGGTGTTCCT GCTCAACTTC
    CCGCTGTACA TCGCCCACAA ACATGGCATG CACTCTCGGC TGGACGACCT GCAGGCTGAG
    GAGGCCCACC ACCTGGCCCT CATCTGCGAT CTTCACGACA ACGACCTGCC CATCTATCTG
    CTGCGGAATG TGAGCCTGTT CTGCAACTCG GGCGGCTTTG GCGCCATGTC GCTGTGCTTC
    GAACATCCCG ATCTGCCAGT GTCCACCGCT CACTCGATGA CCGCTGCCGT CTCAAATGTG
    AAACTTTGGC TGAACTATCA CTGTAATACT CAGCTCTTCG TGCCGCTGCG AAGTCGGATA
    CTGCAGTACA TGTGCAAGCT GTCGGATCAG AGCCTCCGAT CTGCCGCAAC CCGCGCGATG
    GCCGATTTTG TGTGGTCCTC TATGCGAGAC CCACTGGATG TTGCTGTCAA CTTCGACACC
    GAAGGATTGG CCCTGGCCTT TAAGTACTTC ACATCGACCA CGCTAACCAT GCGACTGGCA
    GGCATGGCCC AGATCAACGC ACACATTAAT CTATTTAACG AGATCTGCAC CACCGAAACG
    GTCAACGAAG TGGAGCTGTT CGGGCAGCGC ATCGCGAACT GGCTGACTGA AAATCATATT
    GTACAGCACC TGTTTGGCCC GAATCTGCAC GTAGAAATCG TTAAACAGGC ACACGTGCTG
    CTCAACTTTT TGGCGGTAGA GAACCAGATC TCTGAAGAGG ACATCAAGCT GATCTGGCAA
    GCCACCCAGC TGAAGCACTG CTCAAAGACG ATCTTCGACA TCCTGCCTTC GCTGGTGAAG
    AATCTGACCC CGCGCCCGGC AATGCACCTT TACTCCCTCC TTTGCCGCAT GGATCCCAAG
    GAGCACACTG AGCAGAGCAT ATACATAGCC TCAGCGCTAA CAAAACAGCT GTGGACTCGT
    GATACCTCAC GCAGTCAGAT GAACCTGATG CAAGACCACC TGCTGGGGTC CAACGTCACC
    GCTTCCAGCT CTGACAGCGG CAGCATTGAG GGCAGCAACA CAGAGGACGA CCACGTGGGC
    GCCGACGACA GTAGCATTGC CAGCGGTGGC GGCGGCGGAG GTGTTGGTAA CAAGAGTCCA
    ATCGATGGAG TTACTCCATG CAAGCAGGCC CGGCACAGGC GGCACATCTG TGACCCCACC
    ACCGAGAAGG GAAAGCAAAT AAGTCCCGAG GATATGGCCA AAGTAGACTT GGTGAACAAA
    CGGATTGTGA ACATTATCGA CAACACAAGC AGTGAAGAGG AGCTGCAATC GCGGGCCGCA
    CTGGAGCTGC AACTGCATCG CAGCCGCAAA AAGACGAGCA ACAAGCGACG GCGACAAAAA
    ACCAACAAAC AGATCATTCT GCCCCATGAG CTGGTCGAGA TTTGGGACGG AGTTGAAGAT
    GTGCCCAGCA GTGACGAGGC TGATGGCGAA GCCGATGGTG ATGGCGAGGG CGAACTTTTA
    GCGGACAGCG ATGAGTGCAG CGATGGTGCT ACGAGTCAGC TGGTACCCGA CGCCGTGCTA
    AAGCATCTGC AGGGAGAGGG ACCTTTTATC AGGGCCATCG AGACCAATAT CAATGAGCTG
    CTCAGTGGTG CCGAGAACGA TGGCAGTTAC TCATCCCCGA TGAGTAACAA ATCTGAAAAG
    AACCTGGCTG ACTTCGATGA CGAGGACGTG TCGCCTTGCG AGGAGGAGCT GGCCCAGCTG
    GTCAGCTCCC GCGCCAATTG TTCGGACGTT CCACCAGCCT TTGCAGCCGC TGCCGCCGCC
    ATGATGGTGG CCCAGTCGGC GATGGCCCTT CAGAAGTCTG GAGAGTCGAA TGTGGCTGCT
    GCTGCCGCTG CTGTGGCCGC TGCTGCAGCG GCTACCGGAA CTGCACAGCA AACGCTGGTG
    GCAATGAACC GGCAGGCCAG CGTTGCTGCA GCTGCCGCCG TGGTTGCTGC GGCCAAGGCA
    AAATCGGACT CAGATGTCGA TCTGATGGAT GTGGTTTCCG GCGGAAAGCA GCACCATTCA
    CCAAAGGCGA GCCAGGGCAG CTCTACGTCC GGGAGTACAC CGGTGCAGCC CTCGTTCAAG
    CTAAATGATG TCTGCCAGCC CGGAAACACA CTGCTTTGGG ATCTGCTTCA AGACGATAAA
    ATTGGCCAAC TAGGCGAGTC TCTGGCTCTA GAGGCGGAGA AAGCTCTGGC CACTCTCCTG
    TGCTTCAGCA TGGACCGCCA ACTGCGAACG AAATTCATCG AGGGTTGTCT GTATAACGTC
    GCCAACAACC GGAGTGTTAT TGTCAGTCTG CGACTGCTGC CCAAACTGTT CGCCTCGTTC
    CAACAGTTTC GACCCTCGGA CACGCACTCT ATGACGATGT GGGCAGAGCG AAATCACCGG
    ATGATGCAGT GCTTTTTCAA TAATATACGT CACTATGCGC GTCGCCATGC CGAGGTACTG
    ATCACCCAAA ACGGAGAGCA GCAGCAGCAG CAGCTGGGCG GACAACTCTA CTCGCACAAG
    ACACAAGTTT CTGTAAGACT GCAGTTCCTA TCATCGATAT TCTCGACGGT GGGATCCCCG
    AAAAGCTTCC GCCTAACGCT GGAGCAGCTT GACGCGCTAT GGGAGTGGCT GGCCCACGAT
    CCGGAGTGTG CCGACTGCTA CTTTTCGTGG CTGCAGGCCC AGGCCAAAGG CGGTGATCAG
    CATGCTCTGG GCATTGAAGC ACTTCAGCAT TTGTATTTGA AGAAACTCCC AGAGCTGCGA
    CCCGAGGAGT TCTCTATGGT TGCGTTGGGA CTATTTCAAC AGCTCTGTAG CTTCGCCCGC
    ATCGCCATGG CCGAGTACGA CAATCATAGC GACCAGATCT CGGCGAGCGC CAGTGCCGTT
    GGCATGTACC ATCTTTGGAA GATAGCGTTG CGTGCCCAGA GCAACGATGT CTCCTTGGCG
    GCCATACAGT ACATCAACAT GTACTACATG GGTCAACAAT TGCGCCTGGA GAAGGAGTTC
    GTTTCACAGT GTATGGAAAA CCTAGTTCAA GCGGCCACGG CTTTGGAAAG CATCGATGAT
    GAGAATGCTT TGATGCGTGT CCAGCGTGGC CTGCTCTTGC TGAACACCCA TTTGGATACG
    TTCCGGCGTC GATATGCGTT TCATCTGCGC CGCTGGGCCA TCGAAGGCAA AGGGATTGGA
    TCGCACAGTA ACCTTAAAAA TGAGGGAGCG GGTCCACCAC TACGAATAGT GCTGCAACAG
    GCAGGACTTT CGGAAAAAAG CCTTTTGCAG ATGCACGCGT GTGACTTAAT CGCCGACCTC
    AAGGCTGAGG TGTCCAAGTG GTGGGAGAGC CTTCAAACTG GACTAGCTGC TCCTGTGCTC
    GGTCTGCTGC TCAGCGATGG ACCGCTACGC ATTATCACAC AGGGACAGGA ATTGACCTCG
    GATTACGATG AACGGAGTCT CGGAGATGCT GGTTTCAAGG ATAACCAGAT CGTCTACGTA
    TCGTTAGGTG GACGTGGAGC CAGGCGAAAG GAATCCAATT TGGAGCATCC GTCAATGCTG
    CCGCCCCCAC CAAAGGAGTG TTTGCCCACG GTGCTCCTGC TGCAGCCAAA GTACTTCGAG
    AAGCTATTTT GCCTGATGCA GACCCTGGGC GATATGCAAC CGCAAGCGTC GACTGTAAAT
    CCCCAGCACC ACACAAAGGC GCAATTGCTC TCGCGACGCG TATGGGATAT CCTCGCCATG
    CTGCCAACTA ATCCGCACAT TCTAGACGCC TTTAAGAGTC TGGTTACGGA CCTAAGCGAA
    CTCGAGCAGC TTGATGCTGG CGGAGAGGAG GAGCAACTGG CCACCAAGCG CAAGCAAATC
    AAGCAGAAGT TCCGAGATCT TCTCGACCCG AACAACTTGC AAAAGTTCAT GTACTCCCTC
    CATATTGTGG AGAGTCTGGC CCTAACAAGT TCCAGACGTG GTGAATCCAA CGGCAATGTG
    GCCATGGGAA ATACACCGGA ACAGGTACGG GTGAAGAAAT CCTCGATGGG AAGACGGAGA
    AATAGCAACG AGCAGCCGCC ACCGCCGCCG CCAGAAGTCA AGATGAGCAA GGAGCAACTG
    TGCGAGCTGG AGGCCCCACT GACGCCCACA CCCTCGACTG GCTTGCAGGA TGTGGAAACG
    GAGGCCAGCA GTAGCAGCGG TGGCGACAAA GAAAACCAAC CGAAACAACA CAGCAAGCGT
    CAGAAGAAAG GCGAAACTTT TGAACAGGAA AAGGAGCGCC CAGTGGGTTG CTCCACGCCG
    CCGTCTCCAA CTCCTCCGCC ACCAGCACTT TCGGTAGTGG AAAGAGGTGA CAATAAGTGG
    AGTGAGGCGT TCGTCAAATG TGGTGGCCTT CGTCATCTGT ACGAAATCTT TAGTGAGGGC
    CAACTACAAC AAAGCGCACA TCCCAAGGAG TTGGCTTTAA ATGAATGGCG ACACGATTGC
    CTGGCCAGCC TGCTGCGCAT TCTCTGGCTT CTTGGATTCG AGGAACTGCA GTCGGCGGAT
    GCCCATGTTC TAATGTCACG TCCGCACCCG TTTATGCTAC AGTTGATGGA GGTACCACAG
    TGCCTGACCC GACTCTCATC GATCCTTAAC GACGAAGTTC ACCAGCAGCA TCAGGCTTCT
    TCGGCCAATC CACTGGTATT CCCCTATCAG TTCCAGCACT TGCGTACTGG CTTTTGGGGC
    AGGGCCCAGC TCATCCAGTT CGCTATGAAC ATTCTAGTGA GTTTTGTGCA CGCCTCAGCG
    GAAGCGAGAA GGCTTCTCTG GGCCCCAACT GGTACCGATC ACTGTCGATG GCTGCAGAAG
    TTCATCCTGG AGGATCCCGA ACCGGCGGTA CGGCGTGAGA TATGCGCTGG TCTTTATCGC
    ATTTGCTTAG GAAACGCACA TAGCTATCGC CTCTTACTAG CACCACTGCT GCACAAGCTC
    ATCGCCCTGT TGCCATTGGC CGAGCAGATG AGCTCTGGCA ACCAGCACAC GCAGTTTTTG
    CTCTCGGAAG AGGGCAAGGA TCCCTACGGA CCCGCCTGCC GTGACTACTT TTGGCTGCTG
    GCCAGATTGG TGGATACTCT CTCCCCGGAA ATGGTGGCCG AGGAGCACAT CGACATTGAA
    ATGCTGTGCG AAAGCATCTC GCAGAGCATT CTCACCCGAG AGTATTATGA ACTGCGCCAT
    GGTTACCAGG ACGATGGCTT GGTGGGTCTC CTAAATCTCA TGTCCAATTT GATCAAGTAC
    GACACCACAT TCAAGTACAC ACCCAAGGCG TTATCCTTTA TTGAGCAACT GATTGGCTTC
    CTGTTTGACA TGCCCTCGCC GGCAGATCGC CAGAAGCCCA AGTGCAAGTC GGCGTCGTCT
    CGAGCATCTG CCTACGATCT GCTGGTTGAA CTTTGCCGTG GATGCGCCAC AAACTATGCA
    TATCTGCATG GACGTCTGTT GGCACAGCAC AAATCCGGAC CCAAGCAGCC GTATCCCTGG
    GACTATTGGC CGCGCGATGA AGGCAGAGCT GAGTGCGGAT ATGTGGGGCT AACTAATTTG
    GGAGCCACTT GCTATATGGC CTCTTGCGTC CAGCACTTGT ACATGATGCC ACAGGCGCGA
    GCAGCTGTTT TGCGAGTTCC ACCCAACGCC GCCCGTAAGC ATGGGCCCAC ACTTCTGGAA
    CTACAACGGA TGTTTGCCTA CCTACTGGAG TCGGAACGGA AGTCTTACAA CCCGCGAAGC
    TTCTGCCGGG TGTACCAGAT GGATCACCAG CCGCTAAATA CGGGAGAGCA AAAGGATATG
    GCAGAGTTCT TTATCGATTT GGTGTCCAAA CTGGAAGATA TGACGCCTGA CTTGAAGCAC
    CTGGTGAAAA GGCTCTTCTG TGGATCCTTG AGCAATAATG TCGTCTCCCT GGATTGCGGT
    CACGTTTCCC GCACGGCCGA GGACTTTTAC ACGGTTCGCT GCCAGGTTGC AGATATGCGC
    AATCTGCAGG AATCCCTGGA CGAAGTCACC GTTAAGGATA CCCTCGAGGG CGACAACATG
    TACACCTGCT CGCAGTGCGG CAAAAAAGTA CGAGCCGAGA AGCGGGCTTG CTTCAAGAAG
    CTTCCCCAGA TCTTGTGCTT CAACACCATG CGCTACACCT TCAATATGGT CACCATGCTC
    AAGGAGAAGG TCAACACGCA CTTTTCCTTT CCGCTACGCC TGAACATGTG TCACTATGTG
    GAGAAGACCT TGATGCCGCA GCAGTACAAA GAGGAGCGAG AAAGGCGCCA AAAGGAGAAG
    GAAGGAGCAG ATGGTAGTGG CGATGGGAAT GACAACGAAA AGGCAGAGGC CACGCTAGAT
    GACGATATTG AAGAATGCTA CGAGTACGAA CTGGTGGGCG TCACTGTCCA CACGGGCACA
    GCGGATGGCG GCCACTACTA CAGCTTTATA AAGGAGCGGA CGAAGACTAG TTACCATACG
    CACGAGCGGT GGTTTCTTTT CAACGATGCC GAAGTGAAGC CTTTCGATCC CTCACAGATA
    GCGGCGGAGT GCTTTGGCGG CGAGATGACG AGCAAAACCT ACGACTCAGT GACGGAGAAA
    TATCTGGACT TTAGTTTCGA GAAGACCAAC TCTGCCTACA TGCTGTTCTA CGAGCGCCGA
    CTGCCAGAGC ATCTGCAGCG CCGGCATTCC GAGCTGCTGG TTACACCCAC GCCCAGTCCC
    ACAGTGGAAG AGAAGTCCGA GGCGGAGGAG CCCACTAAAA TGGAGACAAG CTCTAGCGAA
    ATCAAGGCAG ATGTGGATGT GGAAGTGGAA GTTGAGGAGA AGGACAAGGA GAAGCCTGCG
    CAGACTGACA CAGAATCTAA GGAAACGCCT GCAAAAGAGG AGATCGCGGA TGACAAATCG
    AAGCAGGATG AGCCTGAGGA AAAGAAAATC GAAAAGCAAT CCAGAGAGGG CGAAGAGAAA
    TCGGAAACGG ACGAAAAGCC CACGGAGATG ACGACCGTGA GCACTGAGGA GGAAAAGCAG
    CCAACAGCCA ATTGTGATAA TCACCAGCAA AACAACAATA GCAACAGCAA AGCCAGCAAC
    GATCAGCAAC CATCCACGTC GAAGGCTGCC CAGAAGCTAC AACTATTTCG TCCATTGTTG
    AACAAGGAGC TCGAGGATTG GATTTGGCAG GACAATCGAC AGTTCCTGCA GGATCGCAAC
    ATTTTCGAGC ACACATATTT CAACTTTATG TGGCAGATAT GTGGGCACAT ACCCCAATCA
    TTAATATCAG AGACGGACGT CACCTGCATG GCAGCCAAGT TGTCTGTGTC CTTTTTCATT
    GAGACTTTTA TTCATGCTAA GGAAAAGCCC ACTATGGTGC CCTGGGTGGA GCTGCTGACC
    AAGCAGTTCA ATGCCAGCCA AGAGGCCTGC GAGTGGTTTC TGTCGCACAT GTCGCAGGAG
    CCCTACTGGC CGGTCCAGGT GCTAATCCAG TGTCCCAACC AAATGGTGCG TCAAATGTTT
    CAGCGTTTGG TAATCCATGT CATCCAGCAA CTACGTGCCT CACATGCTCA TCTGTATCTG
    GAAGTGGAGA CAGACGAGGA TGACAAGGAG TTAATAGGCC AAGCCTCTTG CGTCACCCGT
    TTCATTGGTT CGCTCATCTC GCTCCTGGAG CACGGTGCCC GAGCGAACCT GCGGCACTTG
    TCCGAGTACT TTGGACTGTT GTGCGAGTTC TCACGGATGG GAGATGAGGA AGCCATGTAC
    TTGCTGCGCA TCGGTGTACT CAAGAGCCTG GTCGACTTCT ATCTGGGTCA CAAGCAAACA
    GACAGCATAG ACATAAGCTC TGACAACGAA GACAACTCCT CTGAAGAGGC GCTGTCCGTG
    GAGAAGATGC GTCCGGCCTC GCTGGACAAG ATGATTGCTC TGTGCGCCAG CCTAGTGGAA
    AGATCAAGAG GAGCCGACTT CCGGCTGCGA CTCTCGCCCA AAGACTTCTC TGCAATCGCC
    GGCGGCAAGG GTTTTCCATT TTTGTACCAA CAAATAAAGG ACGGCATTAA TCCGCACCAG
    ACAAAGCATT TAATACACGC GCTTTGTCGC TGGGATGAGC GCCTGGCCAC GCAGATCATT
    GGCATGCTCT TCGCGTCGGT AACCAAGCAC ACGGAGCTGT GCGCGCCATT CTTCAAGCTG
    CTAACTCTGC TCACCGAGAC GCAGGGCGGT CCAGTGGGCC TGCCTTGTTT CACCCAGTTG
    ATCCTGCCAC GAATGTGGGA CGCCGCCGAG TACTGTCCAC AGTCGGTGCT CGACTGGCTC
    TCGCTGCAGG CCACCAAGAA CAAGATCGCC CACGCCTGGA TACTTCAATC GGCCGAGAAG
    TGGCTCGAGC AATTCCTGCT GGCCCACGAT AACACTCGTG TTCGGAACGC TGCCGCCTTT
    CTGCTGGTGG CACTGGTACC CTCCCAGCCA TTCCGGGCCA ACTTCCGCGC CCACTCGCAA
    CACAAGCTGC TGGCCCTCAA TCCGCACAGT TATCGCGACA TAAATAGCGA TGCCCAGGCG
    GTGCTGCACC AGGTGATCAC GCTGCTCCTG CGATTGCTGC GTCCAGCGCG CGTCTATGCG
    GATATCGGAG CCCATGGCAC GACCAAGCTG ACCGCGTACT TTAATCTGCT CAGCTATTGT
    ATGGTTTCAA AGACTGAAAA ACTAATGGCA AGTTCGTACA TGCGCTCCCT GTGGGAGCTG
    TTCCATCCAC GCCTCTCGGA GCCCAGTGTT CCGGCCCATC ACAATAAGCA CGCACTATTA
    ACCTTCTGGC ACCACTCACT GGTGGACTGT CCGGAGAATG CTGCCCAGGT GGCCAACTGC
    CCGGAGATTA CGCGCAACAT TGCATTTAAC TATATACTGG CCGATCATGA TGACGCGGAG
    ATAGTCACCT ACAACCGTTC CATGCTGCCC GCCTATTATG GACTACTTCG TCTGTGCTGC
    GAGCAGAGCC GTGCACTCAC ACGCCAATTG TCCCAGCATC AGAATCTTCA GTGGGCATTT
    AAAAACATCA CACCGCATCC CACGCAGTAC GCGGCCGCCG TGGACGAGCT TTTCAAGTTG
    ATGGCTCTGT TCGCCACCCG TCATCCGGAT GCTAGTGAAC AGGAAAAGCT TGATGTGACC
    CAGTTTAGGA GGGCTGTGAT TGTGTCCTAC ACCAGTAGTC TGGATGCACG TGTCTCTTGG
    TCCACGCTGA TCAGTGCTCT TAAGATACTC GTGGACAATG AGGAGGACCG CACGATGGTG
    ATCTTCAACG GCGGCATTGA GATGTGTTTC GAGGCGCTGC ACACGCTACA TTCCATGCAC
    CACGAGGCCA CCGCCTGCCA CGTGGCCGGA GACCTATTCG ATCTCTTGGG CGAGATGCTA
    CTCCTCCTAG CCACTCTTCG AACCCGCACA GACAGTCCTG CTCAAAAGAA GCAGCAGCAA
    CAACAGCAGC AGCTGGAGCA ACAGTTGCAG TTGCAGCAGC AACAGATGCT GCAGAAACAA
    CAGCAGCAGT CGCAAAGCCA AACGCAAACA CCGCAGTCGC CGCAGCAAAA GGAAAAGCAG
    CTGCAGCAAC AAATGCAGCA GCATCTGCAG CTGCAACAGT TGCAGCAAAT GCAGTTCCAG
    CAGCAACACT TCCTGCGCCA GCAACATCAA CACTCGGCGC TGGCAAAGGC ACTGCCGGAT
    GCAGTGAAGC GACTGGCTAC GCTACTCAAC ACCTTCAATT CGCCAGAGAT CAGTCGCATG
    GCCTTGGAGG TGCTCAAGGA GCTGGTGCGC AATCCCAGCC TGGAAACCAT TAGTATTCTG
    GCGCCCATTC TTATCAACTG TCATCTGTCC GTGGCGAATG CTCCCAATGC GATCGGTCCT
    CTGGGACCGT ACTTTCCGCG TCGCGGTGCG AAGCATACGC CTTGGCCATT GGGAGCCAAG
    AACTCACCCC GTCCACCGCG GCCCATGGTA CAGATGTGCA TCGCTCTGGC TGAGCTGACA
    CCACGCGGTC TCGATGCCGA TTACGACGTT CAGGTGGAGT CCTTTTACCG ACCCTACCAT
    GACTTTATCG ATGTCATGAT GCGCATGTGC GTCAACACGG GCACTCTGAA CGACACGCTA
    GTCAAGCTGC AATGCTTGGT GGCCATCGAG TCCACGCCGC TGCACTTCAC CTATTTCCCC
    AAATTCTGGG TGGGCATACA CAACAATGCT CTGACGCACA AATACGTTGA GCTGCTGGTG
    AAAAATCAAC TGTTGGTAGA GTACCTTCAC AATGTCCTTA GGGACGAGCG AAGCATGTTG
    AAGGATGCCT GCGTTCGTGA ATTTCTGGAG CTCTATTATC ACAAGGTTGC CGCCCAGCTG
    CCAGTGGCCC GTATGATCTA CACAATCAAC TATGGTATGC ACTCCAAGGA CGACATTGAT
    GAGCTGTGTG GCGATCTGTT CGCCATCCGA ATCATTGCCC AGGCCACTGG AGTGCCGGCC
    TCAGTGCGCA AGGAGTTGCG AGGATCGCTG AGGGCGCTTC AGAACAAGAG CGAGCGGTTT
    AGGAAGGAAT CGGAGAGGGA TCCATTTCCG AACAAGAAGC AGAAACGTGA CTCGCAAAAG
    ATCAAGGAAA AGGAGCACCC TCAGCCGGAG AGCGAGAAAG AGACCTCAAC GGAGAACGAC
    AAACCATCAG ATGTTTCCAT GGAATCCAGC GGTAACGCGG AGCAAGCAAC GGACAGCACA
    AAACCACCAA CTCCTGCAGG CAGCGACGAT GAGCAGATGG ACAAAACTTC AGTTCCCTCG
    TCCGATGACG AGACAGAGTT GGAGGACGAG CTGCAGCCGA CGCCCAAGCG CAACAAGAAG
    GCTTCGAATA AGAAGACCGC CCAGGATCGT GTCAACGAGG AGCGCGAGAA GCGACTTATC
    ACGCTGATCA CCATGGAATC ATATATCCAG AGCATTTTTG CCATCCTTAA GAGAGATGCC
    AGTGTCCCCA GTTCGGGAGC GACTAAGGAG CCCAGTGAGG AACCGTGTGG CGAGGCATCC
    ACTTCGGCGG CTGCCGCTGT AAAGTTATCT CGTCCCAAAG GAGCCTGTAC TCCGCCGGAG
    CCAATTACCG ACGTAAACGA TACGCGTTGC AACATAGATA CTGAGACAGA GGCGGAGGCG
    GATGAGCAAT CTCCAAAGAC GAGCCAGACA AATGGTAGCC AACAGAACGA ATCCCCGCCA
    GCGGCGACCA GTGCAGACAC TGCACCCGCG AATCCTTCAC CAGCTCCCGC GGCCGCCGTT
    GCGTCCACAT CCCAGGCGGC AAGTCCAACA CAGATTTAGT TGTCATTCAA CGATTTGCTC
    AGCATTTTGT TTTTCGGCAA GCCATTGGAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa07739
    Clone ID Related Accession (Same CDS sequence) NM_001275993.2
    Accession Version NM_001275993.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product puffyeye, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001275993.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    ATGTGCGAGG TGTGCGCCGA CTTCCAGAAT CTCCTGGAGC TCTATGAGGT GCGCGTGGCC 
    AGCTCGGATC TGAAGTTCCA GCTGCTGCTG AAGAGCGAGA TCGAGACCAC CTTCAACTAC
    ATCCAGTCGT GGCCGCAGCG TCAGTGCATG TGCCTGTACC GGGACACCAA GAACTATGAC
    CGTTTCAACC TGGTAGTGCA GTCGCTCATC TGTCTGACGG TGCAGCATCT GAAGCACATC
    GACCACCTCA TCGACAACTA CAAGCGGCTA ACGGCCAGTG CTGCCCAGGT GGTGGCCCAG
    GCCCAGCAGC AGCGTGAACA GCAGCGGGAC GAAGCGTCAG CCCAGGCAGA GGCAAAGGAA
    TCATCCGCGC CAGCGGAAGA ACCTAAGAAG GAGGAGCCAT CTGGGTCCGC CGGTGAGGAG
    GCTCAGGGCT CCGGCGACGG GCCAGCCAAA AAGCCGCCAG TTGGCCCCTG CACTCCCCCT
    CCCCCGCAAA CGGCCAATCC TCAGCACAAA TCCCACCTGC AGTACACAGA GGAGCCCTGG
    ATCCTGCCCG AGGTGGAGAA ACTGTTGGTG CTCGTTTCAA AGGTGTTCCT GCTCAACTTC
    CCGCTGTACA TCGCCCACAA ACATGGCATG CACTCTCGGC TGGACGACCT GCAGGCTGAG
    GAGGCCCACC ACCTGGCCCT CATCTGCGAT CTTCACGACA ACGACCTGCC CATCTATCTG
    CTGCGGAATG TGAGCCTGTT CTGCAACTCG GGCGGCTTTG GCGCCATGTC GCTGTGCTTC
    GAACATCCCG ATCTGCCAGT GTCCACCGCT CACTCGATGA CCGCTGCCGT CTCAAATGTG
    AAACTTTGGC TGAACTATCA CTGTAATACT CAGCTCTTCG TGCCGCTGCG AAGTCGGATA
    CTGCAGTACA TGTGCAAGCT GTCGGATCAG AGCCTCCGAT CTGCCGCAAC CCGCGCGATG
    GCCGATTTTG TGTGGTCCTC TATGCGAGAC CCACTGGATG TTGCTGTCAA CTTCGACACC
    GAAGGATTGG CCCTGGCCTT TAAGTACTTC ACATCGACCA CGCTAACCAT GCGACTGGCA
    GGCATGGCCC AGATCAACGC ACACATTAAT CTATTTAACG AGATCTGCAC CACCGAAACG
    GTCAACGAAG TGGAGCTGTT CGGGCAGCGC ATCGCGAACT GGCTGACTGA AAATCATATT
    GTACAGCACC TGTTTGGCCC GAATCTGCAC GTAGAAATCG TTAAACAGGC ACACGTGCTG
    CTCAACTTTT TGGCGGTAGA GAACCAGATC TCTGAAGAGG ACATCAAGCT GATCTGGCAA
    GCCACCCAGC TGAAGCACTG CTCAAAGACG ATCTTCGACA TCCTGCCTTC GCTGGTGAAG
    AATCTGACCC CGCGCCCGGC AATGCACCTT TACTCCCTCC TTTGCCGCAT GGATCCCAAG
    GAGCACACTG AGCAGAGCAT ATACATAGCC TCAGCGCTAA CAAAACAGCT GTGGACTCGT
    GATACCTCAC GCAGTCAGAT GAACCTGATG CAAGACCACC TGCTGGGGTC CAACGTCACC
    GCTTCCAGCT CTGACAGCGG CAGCATTGAG GGCAGCAACA CAGAGGACGA CCACGTGGGC
    GCCGACGACA GTAGCATTGC CAGCGGTGGC GGCGGCGGAG GTGTTGGTAA CAAGAGTCCA
    ATCGATGGAG TTACTCCATG CAAGCAGGCC CGGCACAGGC GGCACATCTG TGACCCCACC
    ACCGAGAAGG GAAAGCAAAT AAGTCCCGAG GATATGGCCA AAGTAGACTT GGTGAACAAA
    CGGATTGTGA ACATTATCGA CAACACAAGC AGTGAAGAGG AGCTGCAATC GCGGGCCGCA
    CTGGAGCTGC AACTGCATCG CAGCCGCAAA AAGACGAGCA ACAAGCGACG GCGACAAAAA
    ACCAACAAAC AGATCATTCT GCCCCATGAG CTGGTCGAGA TTTGGGACGG AGTTGAAGAT
    GTGCCCAGCA GTGACGAGGC TGATGGCGAA GCCGATGGTG ATGGCGAGGG CGAACTTTTA
    GCGGACAGCG ATGAGTGCAG CGATGGTGCT ACGAGTCAGC TGGTACCCGA CGCCGTGCTA
    AAGCATCTGC AGGGAGAGGG ACCTTTTATC AGGGCCATCG AGACCAATAT CAATGAGCTG
    CTCAGTGGTG CCGAGAACGA TGGCAGTTAC TCATCCCCGA TGAGTAACAA ATCTGAAAAG
    AACCTGGCTG ACTTCGATGA CGAGGACGTG TCGCCTTGCG AGGAGGAGCT GGCCCAGCTG
    GTCAGCTCCC GCGCCAATTG TTCGGACGTT CCACCAGCCT TTGCAGCCGC TGCCGCCGCC
    ATGATGGTGG CCCAGTCGGC GATGGCCCTT CAGAAGTCTG GAGAGTCGAA TGTGGCTGCT
    GCTGCCGCTG CTGTGGCCGC TGCTGCAGCG GCTACCGGAA CTGCACAGCA AACGCTGGTG
    GCAATGAACC GGCAGGCCAG CGTTGCTGCA GCTGCCGCCG TGGTTGCTGC GGCCAAGGCA
    AAATCGGACT CAGATGTCGA TCTGATGGAT GTGGTTTCCG GCGGAAAGCA GCACCATTCA
    CCAAAGGCGA GCCAGGGCAG CTCTACGTCC GGGAGTACAC CGGTGCAGCC CTCGTTCAAG
    CTAAATGATG TCTGCCAGCC CGGAAACACA CTGCTTTGGG ATCTGCTTCA AGACGATAAA
    ATTGGCCAAC TAGGCGAGTC TCTGGCTCTA GAGGCGGAGA AAGCTCTGGC CACTCTCCTG
    TGCTTCAGCA TGGACCGCCA ACTGCGAACG AAATTCATCG AGGGTTGTCT GTATAACGTC
    GCCAACAACC GGAGTGTTAT TGTCAGTCTG CGACTGCTGC CCAAACTGTT CGCCTCGTTC
    CAACAGTTTC GACCCTCGGA CACGCACTCT ATGACGATGT GGGCAGAGCG AAATCACCGG
    ATGATGCAGT GCTTTTTCAA TAATATACGT CACTATGCGC GTCGCCATGC CGAGGTACTG
    ATCACCCAAA ACGGAGAGCA GCAGCAGCAG CAGCTGGGCG GACAACTCTA CTCGCACAAG
    ACACAAGTTT CTGTAAGACT GCAGTTCCTA TCATCGATAT TCTCGACGGT GGGATCCCCG
    AAAAGCTTCC GCCTAACGCT GGAGCAGCTT GACGCGCTAT GGGAGTGGCT GGCCCACGAT
    CCGGAGTGTG CCGACTGCTA CTTTTCGTGG CTGCAGGCCC AGGCCAAAGG CGGTGATCAG
    CATGCTCTGG GCATTGAAGC ACTTCAGCAT TTGTATTTGA AGAAACTCCC AGAGCTGCGA
    CCCGAGGAGT TCTCTATGGT TGCGTTGGGA CTATTTCAAC AGCTCTGTAG CTTCGCCCGC
    ATCGCCATGG CCGAGTACGA CAATCATAGC GACCAGATCT CGGCGAGCGC CAGTGCCGTT
    GGCATGTACC ATCTTTGGAA GATAGCGTTG CGTGCCCAGA GCAACGATGT CTCCTTGGCG
    GCCATACAGT ACATCAACAT GTACTACATG GGTCAACAAT TGCGCCTGGA GAAGGAGTTC
    GTTTCACAGT GTATGGAAAA CCTAGTTCAA GCGGCCACGG CTTTGGAAAG CATCGATGAT
    GAGAATGCTT TGATGCGTGT CCAGCGTGGC CTGCTCTTGC TGAACACCCA TTTGGATACG
    TTCCGGCGTC GATATGCGTT TCATCTGCGC CGCTGGGCCA TCGAAGGCAA AGGGATTGGA
    TCGCACAGTA ACCTTAAAAA TGAGGGAGCG GGTCCACCAC TACGAATAGT GCTGCAACAG
    GCAGGACTTT CGGAAAAAAG CCTTTTGCAG ATGCACGCGT GTGACTTAAT CGCCGACCTC
    AAGGCTGAGG TGTCCAAGTG GTGGGAGAGC CTTCAAACTG GACTAGCTGC TCCTGTGCTC
    GGTCTGCTGC TCAGCGATGG ACCGCTACGC ATTATCACAC AGGGACAGGA ATTGACCTCG
    GATTACGATG AACGGAGTCT CGGAGATGCT GGTTTCAAGG ATAACCAGAT CGTCTACGTA
    TCGTTAGGTG GACGTGGAGC CAGGCGAAAG GAATCCAATT TGGAGCATCC GTCAATGCTG
    CCGCCCCCAC CAAAGGAGTG TTTGCCCACG GTGCTCCTGC TGCAGCCAAA GTACTTCGAG
    AAGCTATTTT GCCTGATGCA GACCCTGGGC GATATGCAAC CGCAAGCGTC GACTGTAAAT
    CCCCAGCACC ACACAAAGGC GCAATTGCTC TCGCGACGCG TATGGGATAT CCTCGCCATG
    CTGCCAACTA ATCCGCACAT TCTAGACGCC TTTAAGAGTC TGGTTACGGA CCTAAGCGAA
    CTCGAGCAGC TTGATGCTGG CGGAGAGGAG GAGCAACTGG CCACCAAGCG CAAGCAAATC
    AAGCAGAAGT TCCGAGATCT TCTCGACCCG AACAACTTGC AAAAGTTCAT GTACTCCCTC
    CATATTGTGG AGAGTCTGGC CCTAACAAGT TCCAGACGTG GTGAATCCAA CGGCAATGTG
    GCCATGGGAA ATACACCGGA ACAGGTACGG GTGAAGAAAT CCTCGATGGG AAGACGGAGA
    AATAGCAACG AGCAGCCGCC ACCGCCGCCG CCAGAAGTCA AGATGAGCAA GGAGCAACTG
    TGCGAGCTGG AGGCCCCACT GACGCCCACA CCCTCGACTG GCTTGCAGGA TGTGGAAACG
    GAGGCCAGCA GTAGCAGCGG TGGCGACAAA GAAAACCAAC CGAAACAACA CAGCAAGCGT
    CAGAAGAAAG GCGAAACTTT TGAACAGGAA AAGGAGCGCC CAGTGGGTTG CTCCACGCCG
    CCGTCTCCAA CTCCTCCGCC ACCAGCACTT TCGGTAGTGG AAAGAGGTGA CAATAAGTGG
    AGTGAGGCGT TCGTCAAATG TGGTGGCCTT CGTCATCTGT ACGAAATCTT TAGTGAGGGC
    CAACTACAAC AAAGCGCACA TCCCAAGGAG TTGGCTTTAA ATGAATGGCG ACACGATTGC
    CTGGCCAGCC TGCTGCGCAT TCTCTGGCTT CTTGGATTCG AGGAACTGCA GTCGGCGGAT
    GCCCATGTTC TAATGTCACG TCCGCACCCG TTTATGCTAC AGTTGATGGA GGTACCACAG
    TGCCTGACCC GACTCTCATC GATCCTTAAC GACGAAGTTC ACCAGCAGCA TCAGGCTTCT
    TCGGCCAATC CACTGGTATT CCCCTATCAG TTCCAGCACT TGCGTACTGG CTTTTGGGGC
    AGGGCCCAGC TCATCCAGTT CGCTATGAAC ATTCTAGTGA GTTTTGTGCA CGCCTCAGCG
    GAAGCGAGAA GGCTTCTCTG GGCCCCAACT GGTACCGATC ACTGTCGATG GCTGCAGAAG
    TTCATCCTGG AGGATCCCGA ACCGGCGGTA CGGCGTGAGA TATGCGCTGG TCTTTATCGC
    ATTTGCTTAG GAAACGCACA TAGCTATCGC CTCTTACTAG CACCACTGCT GCACAAGCTC
    ATCGCCCTGT TGCCATTGGC CGAGCAGATG AGCTCTGGCA ACCAGCACAC GCAGTTTTTG
    CTCTCGGAAG AGGGCAAGGA TCCCTACGGA CCCGCCTGCC GTGACTACTT TTGGCTGCTG
    GCCAGATTGG TGGATACTCT CTCCCCGGAA ATGGTGGCCG AGGAGCACAT CGACATTGAA
    ATGCTGTGCG AAAGCATCTC GCAGAGCATT CTCACCCGAG AGTATTATGA ACTGCGCCAT
    GGTTACCAGG ACGATGGCTT GGTGGGTCTC CTAAATCTCA TGTCCAATTT GATCAAGTAC
    GACACCACAT TCAAGTACAC ACCCAAGGCG TTATCCTTTA TTGAGCAACT GATTGGCTTC
    CTGTTTGACA TGCCCTCGCC GGCAGATCGC CAGAAGCCCA AGTGCAAGTC GGCGTCGTCT
    CGAGCATCTG CCTACGATCT GCTGGTTGAA CTTTGCCGTG GATGCGCCAC AAACTATGCA
    TATCTGCATG GACGTCTGTT GGCACAGCAC AAATCCGGAC CCAAGCAGCC GTATCCCTGG
    GACTATTGGC CGCGCGATGA AGGCAGAGCT GAGTGCGGAT ATGTGGGGCT AACTAATTTG
    GGAGCCACTT GCTATATGGC CTCTTGCGTC CAGCACTTGT ACATGATGCC ACAGGCGCGA
    GCAGCTGTTT TGCGAGTTCC ACCCAACGCC GCCCGTAAGC ATGGGCCCAC ACTTCTGGAA
    CTACAACGGA TGTTTGCCTA CCTACTGGAG TCGGAACGGA AGTCTTACAA CCCGCGAAGC
    TTCTGCCGGG TGTACCAGAT GGATCACCAG CCGCTAAATA CGGGAGAGCA AAAGGATATG
    GCAGAGTTCT TTATCGATTT GGTGTCCAAA CTGGAAGATA TGACGCCTGA CTTGAAGCAC
    CTGGTGAAAA GGCTCTTCTG TGGATCCTTG AGCAATAATG TCGTCTCCCT GGATTGCGGT
    CACGTTTCCC GCACGGCCGA GGACTTTTAC ACGGTTCGCT GCCAGGTTGC AGATATGCGC
    AATCTGCAGG AATCCCTGGA CGAAGTCACC GTTAAGGATA CCCTCGAGGG CGACAACATG
    TACACCTGCT CGCAGTGCGG CAAAAAAGTA CGAGCCGAGA AGCGGGCTTG CTTCAAGAAG
    CTTCCCCAGA TCTTGTGCTT CAACACCATG CGCTACACCT TCAATATGGT CACCATGCTC
    AAGGAGAAGG TCAACACGCA CTTTTCCTTT CCGCTACGCC TGAACATGTG TCACTATGTG
    GAGAAGACCT TGATGCCGCA GCAGTACAAA GAGGAGCGAG AAAGGCGCCA AAAGGAGAAG
    GAAGGAGCAG ATGGTAGTGG CGATGGGAAT GACAACGAAA AGGCAGAGGC CACGCTAGAT
    GACGATATTG AAGAATGCTA CGAGTACGAA CTGGTGGGCG TCACTGTCCA CACGGGCACA
    GCGGATGGCG GCCACTACTA CAGCTTTATA AAGGAGCGGA CGAAGACTAG TTACCATACG
    CACGAGCGGT GGTTTCTTTT CAACGATGCC GAAGTGAAGC CTTTCGATCC CTCACAGATA
    GCGGCGGAGT GCTTTGGCGG CGAGATGACG AGCAAAACCT ACGACTCAGT GACGGAGAAA
    TATCTGGACT TTAGTTTCGA GAAGACCAAC TCTGCCTACA TGCTGTTCTA CGAGCGCCGA
    CTGCCAGAGC ATCTGCAGCG CCGGCATTCC GAGCTGCTGG TTACACCCAC GCCCAGTCCC
    ACAGTGGAAG AGAAGTCCGA GGCGGAGGAG CCCACTAAAA TGGAGACAAG CTCTAGCGAA
    ATCAAGGCAG ATGTGGATGT GGAAGTGGAA GTTGAGGAGA AGGACAAGGA GAAGCCTGCG
    CAGACTGACA CAGAATCTAA GGAAACGCCT GCAAAAGAGG AGATCGCGGA TGACAAATCG
    AAGCAGGATG AGCCTGAGGA AAAGAAAATC GAAAAGCAAT CCAGAGAGGG CGAAGAGAAA
    TCGGAAACGG ACGAAAAGCC CACGGAGATG ACGACCGTGA GCACTGAGGA GGAAAAGCAG
    CCAACAGCCA ATTGTGATAA TCACCAGCAA AACAACAATA GCAACAGCAA AGCCAGCAAC
    GATCAGCAAC CATCCACGTC GAAGGCTGCC CAGAAGCTAC AACTATTTCG TCCATTGTTG
    AACAAGGAGC TCGAGGATTG GATTTGGCAG GACAATCGAC AGTTCCTGCA GGATCGCAAC
    ATTTTCGAGC ACACATATTT CAACTTTATG TGGCAGATAT GTGGGCACAT ACCCCAATCA
    TTAATATCAG AGACGGACGT CACCTGCATG GCAGCCAAGT TGTCTGTGTC CTTTTTCATT
    GAGACTTTTA TTCATGCTAA GGAAAAGCCC ACTATGGTGC CCTGGGTGGA GCTGCTGACC
    AAGCAGTTCA ATGCCAGCCA AGAGGCCTGC GAGTGGTTTC TGTCGCACAT GTCGCAGGAG
    CCCTACTGGC CGGTCCAGGT GCTAATCCAG TGTCCCAACC AAATGGTGCG TCAAATGTTT
    CAGCGTTTGG TAATCCATGT CATCCAGCAA CTACGTGCCT CACATGCTCA TCTGTATCTG
    GAAGTGGAGA CAGACGAGGA TGACAAGGAG TTAATAGGCC AAGCCTCTTG CGTCACCCGT
    TTCATTGGTT CGCTCATCTC GCTCCTGGAG CACGGTGCCC GAGCGAACCT GCGGCACTTG
    TCCGAGTACT TTGGACTGTT GTGCGAGTTC TCACGGATGG GAGATGAGGA AGCCATGTAC
    TTGCTGCGCA TCGGTGTACT CAAGAGCCTG GTCGACTTCT ATCTGGGTCA CAAGCAAACA
    GACAGCATAG ACATAAGCTC TGACAACGAA GACAACTCCT CTGAAGAGGC GCTGTCCGTG
    GAGAAGATGC GTCCGGCCTC GCTGGACAAG ATGATTGCTC TGTGCGCCAG CCTAGTGGAA
    AGATCAAGAG GAGCCGACTT CCGGCTGCGA CTCTCGCCCA AAGACTTCTC TGCAATCGCC
    GGCGGCAAGG GTTTTCCATT TTTGTACCAA CAAATAAAGG ACGGCATTAA TCCGCACCAG
    ACAAAGCATT TAATACACGC GCTTTGTCGC TGGGATGAGC GCCTGGCCAC GCAGATCATT
    GGCATGCTCT TCGCGTCGGT AACCAAGCAC ACGGAGCTGT GCGCGCCATT CTTCAAGCTG
    CTAACTCTGC TCACCGAGAC GCAGGGCGGT CCAGTGGGCC TGCCTTGTTT CACCCAGTTG
    ATCCTGCCAC GAATGTGGGA CGCCGCCGAG TACTGTCCAC AGTCGGTGCT CGACTGGCTC
    TCGCTGCAGG CCACCAAGAA CAAGATCGCC CACGCCTGGA TACTTCAATC GGCCGAGAAG
    TGGCTCGAGC AATTCCTGCT GGCCCACGAT AACACTCGTG TTCGGAACGC TGCCGCCTTT
    CTGCTGGTGG CACTGGTACC CTCCCAGCCA TTCCGGGCCA ACTTCCGCGC CCACTCGCAA
    CACAAGCTGC TGGCCCTCAA TCCGCACAGT TATCGCGACA TAAATAGCGA TGCCCAGGCG
    GTGCTGCACC AGGTGATCAC GCTGCTCCTG CGATTGCTGC GTCCAGCGCG CGTCTATGCG
    GATATCGGAG CCCATGGCAC GACCAAGCTG ACCGCGTACT TTAATCTGCT CAGCTATTGT
    ATGGTTTCAA AGACTGAAAA ACTAATGGCA AGTTCGTACA TGCGCTCCCT GTGGGAGCTG
    TTCCATCCAC GCCTCTCGGA GCCCAGTGTT CCGGCCCATC ACAATAAGCA CGCACTATTA
    ACCTTCTGGC ACCACTCACT GGTGGACTGT CCGGAGAATG CTGCCCAGGT GGCCAACTGC
    CCGGAGATTA CGCGCAACAT TGCATTTAAC TATATACTGG CCGATCATGA TGACGCGGAG
    ATAGTCACCT ACAACCGTTC CATGCTGCCC GCCTATTATG GACTACTTCG TCTGTGCTGC
    GAGCAGAGCC GTGCACTCAC ACGCCAATTG TCCCAGCATC AGAATCTTCA GTGGGCATTT
    AAAAACATCA CACCGCATCC CACGCAGTAC GCGGCCGCCG TGGACGAGCT TTTCAAGTTG
    ATGGCTCTGT TCGCCACCCG TCATCCGGAT GCTAGTGAAC AGGAAAAGCT TGATGTGACC
    CAGTTTAGGA GGGCTGTGAT TGTGTCCTAC ACCAGTAGTC TGGATGCACG TGTCTCTTGG
    TCCACGCTGA TCAGTGCTCT TAAGATACTC GTGGACAATG AGGAGGACCG CACGATGGTG
    ATCTTCAACG GCGGCATTGA GATGTGTTTC GAGGCGCTGC ACACGCTACA TTCCATGCAC
    CACGAGGCCA CCGCCTGCCA CGTGGCCGGA GACCTATTCG ATCTCTTGGG CGAGATGCTA
    CTCCTCCTAG CCACTCTTCG AACCCGCACA GACAGTCCTG CTCAAAAGAA GCAGCAGCAA
    CAACAGCAGC AGCTGGAGCA ACAGTTGCAG TTGCAGCAGC AACAGATGCT GCAGAAACAA
    CAGCAGCAGT CGCAAAGCCA AACGCAAACA CCGCAGTCGC CGCAGCAAAA GGAAAAGCAG
    CTGCAGCAAC AAATGCAGCA GCATCTGCAG CTGCAACAGT TGCAGCAAAT GCAGTTCCAG
    CAGCAACACT TCCTGCGCCA GCAACATCAA CACTCGGCGC TGGCAAAGGC ACTGCCGGAT
    GCAGTGAAGC GACTGGCTAC GCTACTCAAC ACCTTCAATT CGCCAGAGAT CAGTCGCATG
    GCCTTGGAGG TGCTCAAGGA GCTGGTGCGC AATCCCAGCC TGGAAACCAT TAGTATTCTG
    GCGCCCATTC TTATCAACTG TCATCTGTCC GTGGCGAATG CTCCCAATGC GATCGGTCCT
    CTGGGACCGT ACTTTCCGCG TCGCGGTGCG AAGCATACGC CTTGGCCATT GGGAGCCAAG
    AACTCACCCC GTCCACCGCG GCCCATGGTA CAGATGTGCA TCGCTCTGGC TGAGCTGACA
    CCACGCGGTC TCGATGCCGA TTACGACGTT CAGGTGGAGT CCTTTTACCG ACCCTACCAT
    GACTTTATCG ATGTCATGAT GCGCATGTGC GTCAACACGG GCACTCTGAA CGACACGCTA
    GTCAAGCTGC AATGCTTGGT GGCCATCGAG TCCACGCCGC TGCACTTCAC CTATTTCCCC
    AAATTCTGGG TGGGCATACA CAACAATGCT CTGACGCACA AATACGTTGA GCTGCTGGTG
    AAAAATCAAC TGTTGGTAGA GTACCTTCAC AATGTCCTTA GGGACGAGCG AAGCATGTTG
    AAGGATGCCT GCGTTCGTGA ATTTCTGGAG CTCTATTATC ACAAGGTTGC CGCCCAGCTG
    CCAGTGGCCC GTATGATCTA CACAATCAAC TATGGTATGC ACTCCAAGGA CGACATTGAT
    GAGCTGTGTG GCGATCTGTT CGCCATCCGA ATCATTGCCC AGGCCACTGG AGTGCCGGCC
    TCAGTGCGCA AGGAGTTGCG AGGATCGCTG AGGGCGCTTC AGAACAAGAG CGAGCGGTTT
    AGGAAGGAAT CGGAGAGGGA GCTATCATCA TCTCCCTATT TGCCAGATCC ATTTCCGAAC
    AAGAAGCAGA AACGTGACTC GCAAAAGATC AAGGAAAAGG AGCACCCTCA GCCGGAGAGC
    GAGAAAGAGA CCTCAACGGA GAACGACAAA CCATCAGATG TTTCCATGGA ATCCAGCGGT
    AACGCGGAGC AAGCAACGGA CAGCACAAAA CCACCAACTC CTGCAGGCAG CGACGATGAG
    CAGATGGACA AAACTTCAGT TCCCTCGTCC GATGACGAGA CAGAGTTGGA GGACGAGCTG
    CAGCCGACGC CCAAGCGCAA CAAGAAGGCT TCGAATAAGA AGACCGCCCA GGATCGTGTC
    AACGAGGAGC GCGAGAAGCG ACTTATCACG CTGATCACCA TGGAATCATA TATCCAGAGC
    ATTTTTGCCA TCCTTAAGAG AGATGCCAGT GTCCCCAGTT CGGGAGCGAC TAAGGAGCCC
    AGTGAGGAAC CGTGTGGCGA GGCATCCACT TCGGCGGCTG CCGCTGTAAA GTTATCTCGT
    CCCAAAGGAG CCTGTACTCC GCCGGAGCCA ATTACCGACG TAAACGATAC GCGTTGCAAC
    ATAGATACTG AGACAGAGGC GGAGGCGGAT GAGCAATCTC CAAAGACGAG CCAGACAAAT
    GGTAGCCAAC AGAACGAATC CCCGCCAGCG GCGACCAGTG CAGACACTGC ACCCGCGAAT
    CCTTCACCAG CTCCCGCGGC CGCCGTTGCG TCCACATCCC AGGCGGCAAG TCCAACACAG
    ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001262922.1
    CDS279..12044
    Translation

    Target ORF information:

    RefSeq Version NM_001275993.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster puffyeye (puf), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001275993.2

    ORF Insert Sequence:

    1
    61
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    721
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1981
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    2161
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    5161
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    5281
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    5401
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    5881
    5941
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    11221
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    11641
    11701
    11761
    ATGTGCGAGG TGTGCGCCGA CTTCCAGAAT CTCCTGGAGC TCTATGAGGT GCGCGTGGCC 
    AGCTCGGATC TGAAGTTCCA GCTGCTGCTG AAGAGCGAGA TCGAGACCAC CTTCAACTAC
    ATCCAGTCGT GGCCGCAGCG TCAGTGCATG TGCCTGTACC GGGACACCAA GAACTATGAC
    CGTTTCAACC TGGTAGTGCA GTCGCTCATC TGTCTGACGG TGCAGCATCT GAAGCACATC
    GACCACCTCA TCGACAACTA CAAGCGGCTA ACGGCCAGTG CTGCCCAGGT GGTGGCCCAG
    GCCCAGCAGC AGCGTGAACA GCAGCGGGAC GAAGCGTCAG CCCAGGCAGA GGCAAAGGAA
    TCATCCGCGC CAGCGGAAGA ACCTAAGAAG GAGGAGCCAT CTGGGTCCGC CGGTGAGGAG
    GCTCAGGGCT CCGGCGACGG GCCAGCCAAA AAGCCGCCAG TTGGCCCCTG CACTCCCCCT
    CCCCCGCAAA CGGCCAATCC TCAGCACAAA TCCCACCTGC AGTACACAGA GGAGCCCTGG
    ATCCTGCCCG AGGTGGAGAA ACTGTTGGTG CTCGTTTCAA AGGTGTTCCT GCTCAACTTC
    CCGCTGTACA TCGCCCACAA ACATGGCATG CACTCTCGGC TGGACGACCT GCAGGCTGAG
    GAGGCCCACC ACCTGGCCCT CATCTGCGAT CTTCACGACA ACGACCTGCC CATCTATCTG
    CTGCGGAATG TGAGCCTGTT CTGCAACTCG GGCGGCTTTG GCGCCATGTC GCTGTGCTTC
    GAACATCCCG ATCTGCCAGT GTCCACCGCT CACTCGATGA CCGCTGCCGT CTCAAATGTG
    AAACTTTGGC TGAACTATCA CTGTAATACT CAGCTCTTCG TGCCGCTGCG AAGTCGGATA
    CTGCAGTACA TGTGCAAGCT GTCGGATCAG AGCCTCCGAT CTGCCGCAAC CCGCGCGATG
    GCCGATTTTG TGTGGTCCTC TATGCGAGAC CCACTGGATG TTGCTGTCAA CTTCGACACC
    GAAGGATTGG CCCTGGCCTT TAAGTACTTC ACATCGACCA CGCTAACCAT GCGACTGGCA
    GGCATGGCCC AGATCAACGC ACACATTAAT CTATTTAACG AGATCTGCAC CACCGAAACG
    GTCAACGAAG TGGAGCTGTT CGGGCAGCGC ATCGCGAACT GGCTGACTGA AAATCATATT
    GTACAGCACC TGTTTGGCCC GAATCTGCAC GTAGAAATCG TTAAACAGGC ACACGTGCTG
    CTCAACTTTT TGGCGGTAGA GAACCAGATC TCTGAAGAGG ACATCAAGCT GATCTGGCAA
    GCCACCCAGC TGAAGCACTG CTCAAAGACG ATCTTCGACA TCCTGCCTTC GCTGGTGAAG
    AATCTGACCC CGCGCCCGGC AATGCACCTT TACTCCCTCC TTTGCCGCAT GGATCCCAAG
    GAGCACACTG AGCAGAGCAT ATACATAGCC TCAGCGCTAA CAAAACAGCT GTGGACTCGT
    GATACCTCAC GCAGTCAGAT GAACCTGATG CAAGACCACC TGCTGGGGTC CAACGTCACC
    GCTTCCAGCT CTGACAGCGG CAGCATTGAG GGCAGCAACA CAGAGGACGA CCACGTGGGC
    GCCGACGACA GTAGCATTGC CAGCGGTGGC GGCGGCGGAG GTGTTGGTAA CAAGAGTCCA
    ATCGATGGAG TTACTCCATG CAAGCAGGCC CGGCACAGGC GGCACATCTG TGACCCCACC
    ACCGAGAAGG GAAAGCAAAT AAGTCCCGAG GATATGGCCA AAGTAGACTT GGTGAACAAA
    CGGATTGTGA ACATTATCGA CAACACAAGC AGTGAAGAGG AGCTGCAATC GCGGGCCGCA
    CTGGAGCTGC AACTGCATCG CAGCCGCAAA AAGACGAGCA ACAAGCGACG GCGACAAAAA
    ACCAACAAAC AGATCATTCT GCCCCATGAG CTGGTCGAGA TTTGGGACGG AGTTGAAGAT
    GTGCCCAGCA GTGACGAGGC TGATGGCGAA GCCGATGGTG ATGGCGAGGG CGAACTTTTA
    GCGGACAGCG ATGAGTGCAG CGATGGTGCT ACGAGTCAGC TGGTACCCGA CGCCGTGCTA
    AAGCATCTGC AGGGAGAGGG ACCTTTTATC AGGGCCATCG AGACCAATAT CAATGAGCTG
    CTCAGTGGTG CCGAGAACGA TGGCAGTTAC TCATCCCCGA TGAGTAACAA ATCTGAAAAG
    AACCTGGCTG ACTTCGATGA CGAGGACGTG TCGCCTTGCG AGGAGGAGCT GGCCCAGCTG
    GTCAGCTCCC GCGCCAATTG TTCGGACGTT CCACCAGCCT TTGCAGCCGC TGCCGCCGCC
    ATGATGGTGG CCCAGTCGGC GATGGCCCTT CAGAAGTCTG GAGAGTCGAA TGTGGCTGCT
    GCTGCCGCTG CTGTGGCCGC TGCTGCAGCG GCTACCGGAA CTGCACAGCA AACGCTGGTG
    GCAATGAACC GGCAGGCCAG CGTTGCTGCA GCTGCCGCCG TGGTTGCTGC GGCCAAGGCA
    AAATCGGACT CAGATGTCGA TCTGATGGAT GTGGTTTCCG GCGGAAAGCA GCACCATTCA
    CCAAAGGCGA GCCAGGGCAG CTCTACGTCC GGGAGTACAC CGGTGCAGCC CTCGTTCAAG
    CTAAATGATG TCTGCCAGCC CGGAAACACA CTGCTTTGGG ATCTGCTTCA AGACGATAAA
    ATTGGCCAAC TAGGCGAGTC TCTGGCTCTA GAGGCGGAGA AAGCTCTGGC CACTCTCCTG
    TGCTTCAGCA TGGACCGCCA ACTGCGAACG AAATTCATCG AGGGTTGTCT GTATAACGTC
    GCCAACAACC GGAGTGTTAT TGTCAGTCTG CGACTGCTGC CCAAACTGTT CGCCTCGTTC
    CAACAGTTTC GACCCTCGGA CACGCACTCT ATGACGATGT GGGCAGAGCG AAATCACCGG
    ATGATGCAGT GCTTTTTCAA TAATATACGT CACTATGCGC GTCGCCATGC CGAGGTACTG
    ATCACCCAAA ACGGAGAGCA GCAGCAGCAG CAGCTGGGCG GACAACTCTA CTCGCACAAG
    ACACAAGTTT CTGTAAGACT GCAGTTCCTA TCATCGATAT TCTCGACGGT GGGATCCCCG
    AAAAGCTTCC GCCTAACGCT GGAGCAGCTT GACGCGCTAT GGGAGTGGCT GGCCCACGAT
    CCGGAGTGTG CCGACTGCTA CTTTTCGTGG CTGCAGGCCC AGGCCAAAGG CGGTGATCAG
    CATGCTCTGG GCATTGAAGC ACTTCAGCAT TTGTATTTGA AGAAACTCCC AGAGCTGCGA
    CCCGAGGAGT TCTCTATGGT TGCGTTGGGA CTATTTCAAC AGCTCTGTAG CTTCGCCCGC
    ATCGCCATGG CCGAGTACGA CAATCATAGC GACCAGATCT CGGCGAGCGC CAGTGCCGTT
    GGCATGTACC ATCTTTGGAA GATAGCGTTG CGTGCCCAGA GCAACGATGT CTCCTTGGCG
    GCCATACAGT ACATCAACAT GTACTACATG GGTCAACAAT TGCGCCTGGA GAAGGAGTTC
    GTTTCACAGT GTATGGAAAA CCTAGTTCAA GCGGCCACGG CTTTGGAAAG CATCGATGAT
    GAGAATGCTT TGATGCGTGT CCAGCGTGGC CTGCTCTTGC TGAACACCCA TTTGGATACG
    TTCCGGCGTC GATATGCGTT TCATCTGCGC CGCTGGGCCA TCGAAGGCAA AGGGATTGGA
    TCGCACAGTA ACCTTAAAAA TGAGGGAGCG GGTCCACCAC TACGAATAGT GCTGCAACAG
    GCAGGACTTT CGGAAAAAAG CCTTTTGCAG ATGCACGCGT GTGACTTAAT CGCCGACCTC
    AAGGCTGAGG TGTCCAAGTG GTGGGAGAGC CTTCAAACTG GACTAGCTGC TCCTGTGCTC
    GGTCTGCTGC TCAGCGATGG ACCGCTACGC ATTATCACAC AGGGACAGGA ATTGACCTCG
    GATTACGATG AACGGAGTCT CGGAGATGCT GGTTTCAAGG ATAACCAGAT CGTCTACGTA
    TCGTTAGGTG GACGTGGAGC CAGGCGAAAG GAATCCAATT TGGAGCATCC GTCAATGCTG
    CCGCCCCCAC CAAAGGAGTG TTTGCCCACG GTGCTCCTGC TGCAGCCAAA GTACTTCGAG
    AAGCTATTTT GCCTGATGCA GACCCTGGGC GATATGCAAC CGCAAGCGTC GACTGTAAAT
    CCCCAGCACC ACACAAAGGC GCAATTGCTC TCGCGACGCG TATGGGATAT CCTCGCCATG
    CTGCCAACTA ATCCGCACAT TCTAGACGCC TTTAAGAGTC TGGTTACGGA CCTAAGCGAA
    CTCGAGCAGC TTGATGCTGG CGGAGAGGAG GAGCAACTGG CCACCAAGCG CAAGCAAATC
    AAGCAGAAGT TCCGAGATCT TCTCGACCCG AACAACTTGC AAAAGTTCAT GTACTCCCTC
    CATATTGTGG AGAGTCTGGC CCTAACAAGT TCCAGACGTG GTGAATCCAA CGGCAATGTG
    GCCATGGGAA ATACACCGGA ACAGGTACGG GTGAAGAAAT CCTCGATGGG AAGACGGAGA
    AATAGCAACG AGCAGCCGCC ACCGCCGCCG CCAGAAGTCA AGATGAGCAA GGAGCAACTG
    TGCGAGCTGG AGGCCCCACT GACGCCCACA CCCTCGACTG GCTTGCAGGA TGTGGAAACG
    GAGGCCAGCA GTAGCAGCGG TGGCGACAAA GAAAACCAAC CGAAACAACA CAGCAAGCGT
    CAGAAGAAAG GCGAAACTTT TGAACAGGAA AAGGAGCGCC CAGTGGGTTG CTCCACGCCG
    CCGTCTCCAA CTCCTCCGCC ACCAGCACTT TCGGTAGTGG AAAGAGGTGA CAATAAGTGG
    AGTGAGGCGT TCGTCAAATG TGGTGGCCTT CGTCATCTGT ACGAAATCTT TAGTGAGGGC
    CAACTACAAC AAAGCGCACA TCCCAAGGAG TTGGCTTTAA ATGAATGGCG ACACGATTGC
    CTGGCCAGCC TGCTGCGCAT TCTCTGGCTT CTTGGATTCG AGGAACTGCA GTCGGCGGAT
    GCCCATGTTC TAATGTCACG TCCGCACCCG TTTATGCTAC AGTTGATGGA GGTACCACAG
    TGCCTGACCC GACTCTCATC GATCCTTAAC GACGAAGTTC ACCAGCAGCA TCAGGCTTCT
    TCGGCCAATC CACTGGTATT CCCCTATCAG TTCCAGCACT TGCGTACTGG CTTTTGGGGC
    AGGGCCCAGC TCATCCAGTT CGCTATGAAC ATTCTAGTGA GTTTTGTGCA CGCCTCAGCG
    GAAGCGAGAA GGCTTCTCTG GGCCCCAACT GGTACCGATC ACTGTCGATG GCTGCAGAAG
    TTCATCCTGG AGGATCCCGA ACCGGCGGTA CGGCGTGAGA TATGCGCTGG TCTTTATCGC
    ATTTGCTTAG GAAACGCACA TAGCTATCGC CTCTTACTAG CACCACTGCT GCACAAGCTC
    ATCGCCCTGT TGCCATTGGC CGAGCAGATG AGCTCTGGCA ACCAGCACAC GCAGTTTTTG
    CTCTCGGAAG AGGGCAAGGA TCCCTACGGA CCCGCCTGCC GTGACTACTT TTGGCTGCTG
    GCCAGATTGG TGGATACTCT CTCCCCGGAA ATGGTGGCCG AGGAGCACAT CGACATTGAA
    ATGCTGTGCG AAAGCATCTC GCAGAGCATT CTCACCCGAG AGTATTATGA ACTGCGCCAT
    GGTTACCAGG ACGATGGCTT GGTGGGTCTC CTAAATCTCA TGTCCAATTT GATCAAGTAC
    GACACCACAT TCAAGTACAC ACCCAAGGCG TTATCCTTTA TTGAGCAACT GATTGGCTTC
    CTGTTTGACA TGCCCTCGCC GGCAGATCGC CAGAAGCCCA AGTGCAAGTC GGCGTCGTCT
    CGAGCATCTG CCTACGATCT GCTGGTTGAA CTTTGCCGTG GATGCGCCAC AAACTATGCA
    TATCTGCATG GACGTCTGTT GGCACAGCAC AAATCCGGAC CCAAGCAGCC GTATCCCTGG
    GACTATTGGC CGCGCGATGA AGGCAGAGCT GAGTGCGGAT ATGTGGGGCT AACTAATTTG
    GGAGCCACTT GCTATATGGC CTCTTGCGTC CAGCACTTGT ACATGATGCC ACAGGCGCGA
    GCAGCTGTTT TGCGAGTTCC ACCCAACGCC GCCCGTAAGC ATGGGCCCAC ACTTCTGGAA
    CTACAACGGA TGTTTGCCTA CCTACTGGAG TCGGAACGGA AGTCTTACAA CCCGCGAAGC
    TTCTGCCGGG TGTACCAGAT GGATCACCAG CCGCTAAATA CGGGAGAGCA AAAGGATATG
    GCAGAGTTCT TTATCGATTT GGTGTCCAAA CTGGAAGATA TGACGCCTGA CTTGAAGCAC
    CTGGTGAAAA GGCTCTTCTG TGGATCCTTG AGCAATAATG TCGTCTCCCT GGATTGCGGT
    CACGTTTCCC GCACGGCCGA GGACTTTTAC ACGGTTCGCT GCCAGGTTGC AGATATGCGC
    AATCTGCAGG AATCCCTGGA CGAAGTCACC GTTAAGGATA CCCTCGAGGG CGACAACATG
    TACACCTGCT CGCAGTGCGG CAAAAAAGTA CGAGCCGAGA AGCGGGCTTG CTTCAAGAAG
    CTTCCCCAGA TCTTGTGCTT CAACACCATG CGCTACACCT TCAATATGGT CACCATGCTC
    AAGGAGAAGG TCAACACGCA CTTTTCCTTT CCGCTACGCC TGAACATGTG TCACTATGTG
    GAGAAGACCT TGATGCCGCA GCAGTACAAA GAGGAGCGAG AAAGGCGCCA AAAGGAGAAG
    GAAGGAGCAG ATGGTAGTGG CGATGGGAAT GACAACGAAA AGGCAGAGGC CACGCTAGAT
    GACGATATTG AAGAATGCTA CGAGTACGAA CTGGTGGGCG TCACTGTCCA CACGGGCACA
    GCGGATGGCG GCCACTACTA CAGCTTTATA AAGGAGCGGA CGAAGACTAG TTACCATACG
    CACGAGCGGT GGTTTCTTTT CAACGATGCC GAAGTGAAGC CTTTCGATCC CTCACAGATA
    GCGGCGGAGT GCTTTGGCGG CGAGATGACG AGCAAAACCT ACGACTCAGT GACGGAGAAA
    TATCTGGACT TTAGTTTCGA GAAGACCAAC TCTGCCTACA TGCTGTTCTA CGAGCGCCGA
    CTGCCAGAGC ATCTGCAGCG CCGGCATTCC GAGCTGCTGG TTACACCCAC GCCCAGTCCC
    ACAGTGGAAG AGAAGTCCGA GGCGGAGGAG CCCACTAAAA TGGAGACAAG CTCTAGCGAA
    ATCAAGGCAG ATGTGGATGT GGAAGTGGAA GTTGAGGAGA AGGACAAGGA GAAGCCTGCG
    CAGACTGACA CAGAATCTAA GGAAACGCCT GCAAAAGAGG AGATCGCGGA TGACAAATCG
    AAGCAGGATG AGCCTGAGGA AAAGAAAATC GAAAAGCAAT CCAGAGAGGG CGAAGAGAAA
    TCGGAAACGG ACGAAAAGCC CACGGAGATG ACGACCGTGA GCACTGAGGA GGAAAAGCAG
    CCAACAGCCA ATTGTGATAA TCACCAGCAA AACAACAATA GCAACAGCAA AGCCAGCAAC
    GATCAGCAAC CATCCACGTC GAAGGCTGCC CAGAAGCTAC AACTATTTCG TCCATTGTTG
    AACAAGGAGC TCGAGGATTG GATTTGGCAG GACAATCGAC AGTTCCTGCA GGATCGCAAC
    ATTTTCGAGC ACACATATTT CAACTTTATG TGGCAGATAT GTGGGCACAT ACCCCAATCA
    TTAATATCAG AGACGGACGT CACCTGCATG GCAGCCAAGT TGTCTGTGTC CTTTTTCATT
    GAGACTTTTA TTCATGCTAA GGAAAAGCCC ACTATGGTGC CCTGGGTGGA GCTGCTGACC
    AAGCAGTTCA ATGCCAGCCA AGAGGCCTGC GAGTGGTTTC TGTCGCACAT GTCGCAGGAG
    CCCTACTGGC CGGTCCAGGT GCTAATCCAG TGTCCCAACC AAATGGTGCG TCAAATGTTT
    CAGCGTTTGG TAATCCATGT CATCCAGCAA CTACGTGCCT CACATGCTCA TCTGTATCTG
    GAAGTGGAGA CAGACGAGGA TGACAAGGAG TTAATAGGCC AAGCCTCTTG CGTCACCCGT
    TTCATTGGTT CGCTCATCTC GCTCCTGGAG CACGGTGCCC GAGCGAACCT GCGGCACTTG
    TCCGAGTACT TTGGACTGTT GTGCGAGTTC TCACGGATGG GAGATGAGGA AGCCATGTAC
    TTGCTGCGCA TCGGTGTACT CAAGAGCCTG GTCGACTTCT ATCTGGGTCA CAAGCAAACA
    GACAGCATAG ACATAAGCTC TGACAACGAA GACAACTCCT CTGAAGAGGC GCTGTCCGTG
    GAGAAGATGC GTCCGGCCTC GCTGGACAAG ATGATTGCTC TGTGCGCCAG CCTAGTGGAA
    AGATCAAGAG GAGCCGACTT CCGGCTGCGA CTCTCGCCCA AAGACTTCTC TGCAATCGCC
    GGCGGCAAGG GTTTTCCATT TTTGTACCAA CAAATAAAGG ACGGCATTAA TCCGCACCAG
    ACAAAGCATT TAATACACGC GCTTTGTCGC TGGGATGAGC GCCTGGCCAC GCAGATCATT
    GGCATGCTCT TCGCGTCGGT AACCAAGCAC ACGGAGCTGT GCGCGCCATT CTTCAAGCTG
    CTAACTCTGC TCACCGAGAC GCAGGGCGGT CCAGTGGGCC TGCCTTGTTT CACCCAGTTG
    ATCCTGCCAC GAATGTGGGA CGCCGCCGAG TACTGTCCAC AGTCGGTGCT CGACTGGCTC
    TCGCTGCAGG CCACCAAGAA CAAGATCGCC CACGCCTGGA TACTTCAATC GGCCGAGAAG
    TGGCTCGAGC AATTCCTGCT GGCCCACGAT AACACTCGTG TTCGGAACGC TGCCGCCTTT
    CTGCTGGTGG CACTGGTACC CTCCCAGCCA TTCCGGGCCA ACTTCCGCGC CCACTCGCAA
    CACAAGCTGC TGGCCCTCAA TCCGCACAGT TATCGCGACA TAAATAGCGA TGCCCAGGCG
    GTGCTGCACC AGGTGATCAC GCTGCTCCTG CGATTGCTGC GTCCAGCGCG CGTCTATGCG
    GATATCGGAG CCCATGGCAC GACCAAGCTG ACCGCGTACT TTAATCTGCT CAGCTATTGT
    ATGGTTTCAA AGACTGAAAA ACTAATGGCA AGTTCGTACA TGCGCTCCCT GTGGGAGCTG
    TTCCATCCAC GCCTCTCGGA GCCCAGTGTT CCGGCCCATC ACAATAAGCA CGCACTATTA
    ACCTTCTGGC ACCACTCACT GGTGGACTGT CCGGAGAATG CTGCCCAGGT GGCCAACTGC
    CCGGAGATTA CGCGCAACAT TGCATTTAAC TATATACTGG CCGATCATGA TGACGCGGAG
    ATAGTCACCT ACAACCGTTC CATGCTGCCC GCCTATTATG GACTACTTCG TCTGTGCTGC
    GAGCAGAGCC GTGCACTCAC ACGCCAATTG TCCCAGCATC AGAATCTTCA GTGGGCATTT
    AAAAACATCA CACCGCATCC CACGCAGTAC GCGGCCGCCG TGGACGAGCT TTTCAAGTTG
    ATGGCTCTGT TCGCCACCCG TCATCCGGAT GCTAGTGAAC AGGAAAAGCT TGATGTGACC
    CAGTTTAGGA GGGCTGTGAT TGTGTCCTAC ACCAGTAGTC TGGATGCACG TGTCTCTTGG
    TCCACGCTGA TCAGTGCTCT TAAGATACTC GTGGACAATG AGGAGGACCG CACGATGGTG
    ATCTTCAACG GCGGCATTGA GATGTGTTTC GAGGCGCTGC ACACGCTACA TTCCATGCAC
    CACGAGGCCA CCGCCTGCCA CGTGGCCGGA GACCTATTCG ATCTCTTGGG CGAGATGCTA
    CTCCTCCTAG CCACTCTTCG AACCCGCACA GACAGTCCTG CTCAAAAGAA GCAGCAGCAA
    CAACAGCAGC AGCTGGAGCA ACAGTTGCAG TTGCAGCAGC AACAGATGCT GCAGAAACAA
    CAGCAGCAGT CGCAAAGCCA AACGCAAACA CCGCAGTCGC CGCAGCAAAA GGAAAAGCAG
    CTGCAGCAAC AAATGCAGCA GCATCTGCAG CTGCAACAGT TGCAGCAAAT GCAGTTCCAG
    CAGCAACACT TCCTGCGCCA GCAACATCAA CACTCGGCGC TGGCAAAGGC ACTGCCGGAT
    GCAGTGAAGC GACTGGCTAC GCTACTCAAC ACCTTCAATT CGCCAGAGAT CAGTCGCATG
    GCCTTGGAGG TGCTCAAGGA GCTGGTGCGC AATCCCAGCC TGGAAACCAT TAGTATTCTG
    GCGCCCATTC TTATCAACTG TCATCTGTCC GTGGCGAATG CTCCCAATGC GATCGGTCCT
    CTGGGACCGT ACTTTCCGCG TCGCGGTGCG AAGCATACGC CTTGGCCATT GGGAGCCAAG
    AACTCACCCC GTCCACCGCG GCCCATGGTA CAGATGTGCA TCGCTCTGGC TGAGCTGACA
    CCACGCGGTC TCGATGCCGA TTACGACGTT CAGGTGGAGT CCTTTTACCG ACCCTACCAT
    GACTTTATCG ATGTCATGAT GCGCATGTGC GTCAACACGG GCACTCTGAA CGACACGCTA
    GTCAAGCTGC AATGCTTGGT GGCCATCGAG TCCACGCCGC TGCACTTCAC CTATTTCCCC
    AAATTCTGGG TGGGCATACA CAACAATGCT CTGACGCACA AATACGTTGA GCTGCTGGTG
    AAAAATCAAC TGTTGGTAGA GTACCTTCAC AATGTCCTTA GGGACGAGCG AAGCATGTTG
    AAGGATGCCT GCGTTCGTGA ATTTCTGGAG CTCTATTATC ACAAGGTTGC CGCCCAGCTG
    CCAGTGGCCC GTATGATCTA CACAATCAAC TATGGTATGC ACTCCAAGGA CGACATTGAT
    GAGCTGTGTG GCGATCTGTT CGCCATCCGA ATCATTGCCC AGGCCACTGG AGTGCCGGCC
    TCAGTGCGCA AGGAGTTGCG AGGATCGCTG AGGGCGCTTC AGAACAAGAG CGAGCGGTTT
    AGGAAGGAAT CGGAGAGGGA GCTATCATCA TCTCCCTATT TGCCAGATCC ATTTCCGAAC
    AAGAAGCAGA AACGTGACTC GCAAAAGATC AAGGAAAAGG AGCACCCTCA GCCGGAGAGC
    GAGAAAGAGA CCTCAACGGA GAACGACAAA CCATCAGATG TTTCCATGGA ATCCAGCGGT
    AACGCGGAGC AAGCAACGGA CAGCACAAAA CCACCAACTC CTGCAGGCAG CGACGATGAG
    CAGATGGACA AAACTTCAGT TCCCTCGTCC GATGACGAGA CAGAGTTGGA GGACGAGCTG
    CAGCCGACGC CCAAGCGCAA CAAGAAGGCT TCGAATAAGA AGACCGCCCA GGATCGTGTC
    AACGAGGAGC GCGAGAAGCG ACTTATCACG CTGATCACCA TGGAATCATA TATCCAGAGC
    ATTTTTGCCA TCCTTAAGAG AGATGCCAGT GTCCCCAGTT CGGGAGCGAC TAAGGAGCCC
    AGTGAGGAAC CGTGTGGCGA GGCATCCACT TCGGCGGCTG CCGCTGTAAA GTTATCTCGT
    CCCAAAGGAG CCTGTACTCC GCCGGAGCCA ATTACCGACG TAAACGATAC GCGTTGCAAC
    ATAGATACTG AGACAGAGGC GGAGGCGGAT GAGCAATCTC CAAAGACGAG CCAGACAAAT
    GGTAGCCAAC AGAACGAATC CCCGCCAGCG GCGACCAGTG CAGACACTGC ACCCGCGAAT
    CCTTCACCAG CTCCCGCGGC CGCCGTTGCG TCCACATCCC AGGCGGCAAG TCCAACACAG
    ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa22151
    Clone ID Related Accession (Same CDS sequence) NM_001300579.1
    Accession Version NM_001300579.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product puffyeye, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    ATGTGCGAGG TGTGCGCCGA CTTCCAGAAT CTCCTGGAGC TCTATGAGGT GCGCGTGGCC 
    AGCTCGGATC TGAAGTTCCA GCTGCTGCTG AAGAGCGAGA TCGAGACCAC CTTCAACTAC
    ATCCAGTCGT GGCCGCAGCG TCAGTGCATG TGCCTGTACC GGGACACCAA GAACTATGAC
    CGTTTCAACC TGGTAGTGCA GTCGCTCATC TGTCTGACGG TGCAGCATCT GAAGCACATC
    GACCACCTCA TCGACAACTA CAAGCGGCTA ACGGCCAGTG CTGCCCAGGT GGTGGCCCAG
    GCCCAGCAGC AGCGTGAACA GCAGCGGGAC GAAGCGTCAG CCCAGGCAGA GGCAAAGGAA
    TCATCCGCGC CAGCGGAAGA ACCTAAGAAG GAGGAGCCAT CTGGGTCCGC CGGTGAGGAG
    GCTCAGGGCT CCGGCGACGG GCCAGCCAAA AAGCCGCCAG TTGGCCCCTG CACTCCCCCT
    CCCCCGCAAA CGGCCAATCC TCAGCACAAA TCCCACCTGC AGTACACAGA GGAGCCCTGG
    ATCCTGCCCG AGGTGGAGAA ACTGTTGGTG CTCGTTTCAA AGGTGTTCCT GCTCAACTTC
    CCGCTGTACA TCGCCCACAA ACATGGCATG CACTCTCGGC TGGACGACCT GCAGGCTGAG
    GAGGCCCACC ACCTGGCCCT CATCTGCGAT CTTCACGACA ACGACCTGCC CATCTATCTG
    CTGCGGAATG TGAGCCTGTT CTGCAACTCG GGCGGCTTTG GCGCCATGTC GCTGTGCTTC
    GAACATCCCG ATCTGCCAGT GTCCACCGCT CACTCGATGA CCGCTGCCGT CTCAAATGTG
    AAACTTTGGC TGAACTATCA CTGTAATACT CAGCTCTTCG TGCCGCTGCG AAGTCGGATA
    CTGCAGTACA TGTGCAAGCT GTCGGATCAG AGCCTCCGAT CTGCCGCAAC CCGCGCGATG
    GCCGATTTTG TGTGGTCCTC TATGCGAGAC CCACTGGATG TTGCTGTCAA CTTCGACACC
    GAAGGATTGG CCCTGGCCTT TAAGTACTTC ACATCGACCA CGCTAACCAT GCGACTGGCA
    GGCATGGCCC AGATCAACGC ACACATTAAT CTATTTAACG AGATCTGCAC CACCGAAACG
    GTCAACGAAG TGGAGCTGTT CGGGCAGCGC ATCGCGAACT GGCTGACTGA AAATCATATT
    GTACAGCACC TGTTTGGCCC GAATCTGCAC GTAGAAATCG TTAAACAGGC ACACGTGCTG
    CTCAACTTTT TGGCGGTAGA GAACCAGATC TCTGAAGAGG ACATCAAGCT GATCTGGCAA
    GCCACCCAGC TGAAGCACTG CTCAAAGACG ATCTTCGACA TCCTGCCTTC GCTGGTGAAG
    AATCTGACCC CGCGCCCGGC AATGCACCTT TACTCCCTCC TTTGCCGCAT GGATCCCAAG
    GAGCACACTG AGCAGAGCAT ATACATAGCC TCAGCGCTAA CAAAACAGCT GTGGACTCGT
    GATACCTCAC GCAGTCAGAT GAACCTGATG CAAGACCACC TGCTGGGGTC CAACGTCACC
    GCTTCCAGCT CTGACAGCGG CAGCATTGAG GGCAGCAACA CAGAGGACGA CCACGTGGGC
    GCCGACGACA GTAGCATTGC CAGCGGTGGC GGCGGCGGAG GTGTTGGTAA CAAGAGTCCA
    ATCGATGGAG TTACTCCATG CAAGCAGGCC CGGCACAGGC GGCACATCTG TGACCCCACC
    ACCGAGAAGG GAAAGCAAAT AAGTCCCGAG GATATGGCCA AAGTAGACTT GGTGAACAAA
    CGGATTGTGA ACATTATCGA CAACACAAGC AGTGAAGAGG AGCTGCAATC GCGGGCCGCA
    CTGGAGCTGC AACTGCATCG CAGCCGCAAA AAGACGAGCA ACAAGCGACG GCGACAAAAA
    ACCAACAAAC AGATCATTCT GCCCCATGAG CTGGTCGAGA TTTGGGACGG AGTTGAAGAT
    GTGCCCAGCA GTGACGAGGC TGATGGCGAA GCCGATGGTG ATGGCGAGGG CGAACTTTTA
    GCGGACAGCG ATGAGTGCAG CGATGGTGCT ACGAGTCAGC TGGTACCCGA CGCCGTGCTA
    AAGCATCTGC AGGGAGAGGG ACCTTTTATC AGGGCCATCG AGACCAATAT CAATGAGCTG
    CTCAGTGGTG CCGAGAACGA TGGCAGTTAC TCATCCCCGA TGAGTAACAA ATCTGAAAAG
    AACCTGGCTG ACTTCGATGA CGAGGACGTG TCGCCTTGCG AGGAGGAGCT GGCCCAGCTG
    GTCAGCTCCC GCGCCAATTG TTCGGACGTT CCACCAGCCT TTGCAGCCGC TGCCGCCGCC
    ATGATGGTGG CCCAGTCGGC GATGGCCCTT CAGAAGTCTG GAGAGTCGAA TGTGGCTGCT
    GCTGCCGCTG CTGTGGCCGC TGCTGCAGCG GCTACCGGAA CTGCACAGCA AACGCTGGTG
    GCAATGAACC GGCAGGCCAG CGTTGCTGCA GCTGCCGCCG TGGTTGCTGC GGCCAAGGCA
    AAATCGGACT CAGATGTCGA TCTGATGGAT GTGGTTTCCG GCGGAAAGCA GCACCATTCA
    CCAAAGGCGA GCCAGGGCAG CTCTACGTCC GGGAGTACAC CGGTGCAGCC CTCGTTCAAG
    CTAAATGATG TCTGCCAGCC CGGAAACACA CTGCTTTGGG ATCTGCTTCA AGACGATAAA
    ATTGGCCAAC TAGGCGAGTC TCTGGCTCTA GAGGCGGAGA AAGCTCTGGC CACTCTCCTG
    TGCTTCAGCA TGGACCGCCA ACTGCGAACG AAATTCATCG AGGGTTGTCT GTATAACGTC
    GCCAACAACC GGAGTGTTAT TGTCAGTCTG CGACTGCTGC CCAAACTGTT CGCCTCGTTC
    CAACAGTTTC GACCCTCGGA CACGCACTCT ATGACGATGT GGGCAGAGCG AAATCACCGG
    ATGATGCAGT GCTTTTTCAA TAATATACGT CACTATGCGC GTCGCCATGC CGAGGTACTG
    ATCACCCAAA ACGGAGAGCA GCAGCAGCAG CAGCTGGGCG GACAACTCTA CTCGCACAAG
    ACACAAGTTT CTGTAAGACT GCAGTTCCTA TCATCGATAT TCTCGACGGT GGGATCCCCG
    AAAAGCTTCC GCCTAACGCT GGAGCAGCTT GACGCGCTAT GGGAGTGGCT GGCCCACGAT
    CCGGAGTGTG CCGACTGCTA CTTTTCGTGG CTGCAGGCCC AGGCCAAAGG CGGTGATCAG
    CATGCTCTGG GCATTGAAGC ACTTCAGCAT TTGTATTTGA AGAAACTCCC AGAGCTGCGA
    CCCGAGGAGT TCTCTATGGT TGCGTTGGGA CTATTTCAAC AGCTCTGTAG CTTCGCCCGC
    ATCGCCATGG CCGAGTACGA CAATCATAGC GACCAGATCT CGGCGAGCGC CAGTGCCGTT
    GGCATGTACC ATCTTTGGAA GATAGCGTTG CGTGCCCAGA GCAACGATGT CTCCTTGGCG
    GCCATACAGT ACATCAACAT GTACTACATG GGTCAACAAT TGCGCCTGGA GAAGGAGTTC
    GTTTCACAGT GTATGGAAAA CCTAGTTCAA GCGGCCACGG CTTTGGAAAG CATCGATGAT
    GAGAATGCTT TGATGCGTGT CCAGCGTGGC CTGCTCTTGC TGAACACCCA TTTGGATACG
    TTCCGGCGTC GATATGCGTT TCATCTGCGC CGCTGGGCCA TCGAAGGCAA AGGGATTGGA
    TCGCACAGTA ACCTTAAAAA TGAGGGAGCG GGTCCACCAC TACGAATAGT GCTGCAACAG
    GCAGGACTTT CGGAAAAAAG CCTTTTGCAG ATGCACGCGT GTGACTTAAT CGCCGACCTC
    AAGGCTGAGG TGTCCAAGTG GTGGGAGAGC CTTCAAACTG GACTAGCTGC TCCTGTGCTC
    GGTCTGCTGC TCAGCGATGG ACCGCTACGC ATTATCACAC AGGGACAGGA ATTGACCTCG
    GATTACGATG AACGGAGTCT CGGAGATGCT GGTTTCAAGG ATAACCAGAT CGTCTACGTA
    TCGTTAGGTG GACGTGGAGC CAGGCGAAAG GAATCCAATT TGGAGCATCC GTCAATGCTG
    CCGCCCCCAC CAAAGGAGTG TTTGCCCACG GTGCTCCTGC TGCAGCCAAA GTACTTCGAG
    AAGCTATTTT GCCTGATGCA GACCCTGGGC GATATGCAAC CGCAAGCGTC GACTGTAAAT
    CCCCAGCACC ACACAAAGGC GCAATTGCTC TCGCGACGCG TATGGGATAT CCTCGCCATG
    CTGCCAACTA ATCCGCACAT TCTAGACGCC TTTAAGAGTC TGGTTACGGA CCTAAGCGAA
    CTCGAGCAGC TTGATGCTGG CGGAGAGGAG GAGCAACTGG CCACCAAGCG CAAGCAAATC
    AAGCAGAAGT TCCGAGATCT TCTCGACCCG AACAACTTGC AAAAGTTCAT GTACTCCCTC
    CATATTGTGG AGAGTCTGGC CCTAACAAGT TCCAGACGTG GTGAATCCAA CGGCAATGTG
    GCCATGGGAA ATACACCGGA ACAGGTACGG GTGAAGAAAT CCTCGATGGG AAGACGGAGA
    AATAGCAACG AGCAGCCGCC ACCGCCGCCG CCAGAAGTCA AGATGAGCAA GGAGCAACTG
    TGCGAGCTGG AGGCCCCACT GACGCCCACA CCCTCGACTG GCTTGCAGGA TGTGGAAACG
    GAGGCCAGCA GTAGCAGCGG TGGCGACAAA GAAAACCAAC CGAAACAACA CAGCAAGCGT
    CAGAAGAAAG GCGAAACTTT TGAACAGGAA AAGGAGCGCC CAGTGGGTTG CTCCACGCCG
    CCGTCTCCAA CTCCTCCGCC ACCAGCACTT TCGGTAGTGG AAAGAGGTGA CAATAAGTGG
    AGTGAGGCGT TCGTCAAATG TGGTGGCCTT CGTCATCTGT ACGAAATCTT TAGTGAGGGC
    CAACTACAAC AAAGCGCACA TCCCAAGGAG TTGGCTTTAA ATGAATGGCG ACACGATTGC
    CTGGCCAGCC TGCTGCGCAT TCTCTGGCTT CTTGGATTCG AGGAACTGCA GTCGGCGGAT
    GCCCATGTTC TAATGTCACG TCCGCACCCG TTTATGCTAC AGTTGATGGA GGTACCACAG
    TGCCTGACCC GACTCTCATC GATCCTTAAC GACGAAGTTC ACCAGCAGCA TCAGGCTTCT
    TCGGCCAATC CACTGGTATT CCCCTATCAG TTCCAGCACT TGCGTACTGG CTTTTGGGGC
    AGGGCCCAGC TCATCCAGTT CGCTATGAAC ATTCTAGTGA GTTTTGTGCA CGCCTCAGCG
    GAAGCGAGAA GGCTTCTCTG GGCCCCAACT GGTACCGATC ACTGTCGATG GCTGCAGAAG
    TTCATCCTGG AGGATCCCGA ACCGGCGGTA CGGCGTGAGA TATGCGCTGG TCTTTATCGC
    ATTTGCTTAG GAAACGCACA TAGCTATCGC CTCTTACTAG CACCACTGCT GCACAAGCTC
    ATCGCCCTGT TGCCATTGGC CGAGCAGATG AGCTCTGGCA ACCAGCACAC GCAGTTTTTG
    CTCTCGGAAG AGGGCAAGGA TCCCTACGGA CCCGCCTGCC GTGACTACTT TTGGCTGCTG
    GCCAGATTGG TGGATACTCT CTCCCCGGAA ATGGTGGCCG AGGAGCACAT CGACATTGAA
    ATGCTGTGCG AAAGCATCTC GCAGAGCATT CTCACCCGAG AGTATTATGA ACTGCGCCAT
    GGTTACCAGG ACGATGGCTT GGTGGGTCTC CTAAATCTCA TGTCCAATTT GATCAAGTAC
    GACACCACAT TCAAGTACAC ACCCAAGGCG TTATCCTTTA TTGAGCAACT GATTGGCTTC
    CTGTTTGACA TGCCCTCGCC GGCAGATCGC CAGAAGCCCA AGTGCAAGTC GGCGTCGTCT
    CGAGCATCTG CCTACGATCT GCTGGTTGAA CTTTGCCGTG GATGCGCCAC AAACTATGCA
    TATCTGCATG GACGTCTGTT GGCACAGCAC AAATCCGGAC CCAAGCAGCC GTATCCCTGG
    GACTATTGGC CGCGCGATGA AGGCAGAGCT GAGTGCGGAT ATGTGGGGCT AACTAATTTG
    GGAGCCACTT GCTATATGGC CTCTTGCGTC CAGCACTTGT ACATGATGCC ACAGGCGCGA
    GCAGCTGTTT TGCGAGTTCC ACCCAACGCC GCCCGTAAGC ATGGGCCCAC ACTTCTGGAA
    CTACAACGGA TGTTTGCCTA CCTACTGGAG TCGGAACGGA AGTCTTACAA CCCGCGAAGC
    TTCTGCCGGG TGTACCAGAT GGATCACCAG CCGCTAAATA CGGGAGAGCA AAAGGATATG
    GCAGAGTTCT TTATCGATTT GGTGTCCAAA CTGGAAGATA TGACGCCTGA CTTGAAGCAC
    CTGGTGAAAA GGCTCTTCTG TGGATCCTTG AGCAATAATG TCGTCTCCCT GGATTGCGGT
    CACGTTTCCC GCACGGCCGA GGACTTTTAC ACGGTTCGCT GCCAGGTTGC AGATATGCGC
    AATCTGCAGG AATCCCTGGA CGAAGTCACC GTTAAGGATA CCCTCGAGGG CGACAACATG
    TACACCTGCT CGCAGTGCGG CAAAAAAGTA CGAGCCGAGA AGCGGGCTTG CTTCAAGAAG
    CTTCCCCAGA TCTTGTGCTT CAACACCATG CGCTACACCT TCAATATGGT CACCATGCTC
    AAGGAGAAGG TCAACACGCA CTTTTCCTTT CCGCTACGCC TGAACATGTG TCACTATGTG
    GAGAAGACCT TGATGCCGCA GCAGTACAAA GAGGAGCGAG AAAGGCGCCA AAAGGAGAAG
    GAAGGAGCAG ATGGTAGTGG CGATGGGAAT GACAACGAAA AGGCAGAGGC CACGCTAGAT
    GACGATATTG AAGAATGCTA CGAGTACGAA CTGGTGGGCG TCACTGTCCA CACGGGCACA
    GCGGATGGCG GCCACTACTA CAGCTTTATA AAGGAGCGGA CGAAGACTAG TTACCATACG
    CACGAGCGGT GGTTTCTTTT CAACGATGCC GAAGTGAAGC CTTTCGATCC CTCACAGATA
    GCGGCGGAGT GCTTTGGCGG CGAGATGACG AGCAAAACCT ACGACTCAGT GACGGAGAAA
    TATCTGGACT TTAGTTTCGA GAAGACCAAC TCTGCCTACA TGCTGTTCTA CGAGCGCCGA
    CTGCCAGAGC ATCTGCAGCG CCGGCATTCC GAGCTGCTGG TTACACCCAC GCCCAGTCCC
    ACAGTGGAAG AGAAGTCCGA GGCGGAGGAG CCCACTAAAA TGGAGACAAG CTCTAGCGAA
    ATCAAGGCAG ATGTGGATGT GGAAGTGGAA GTTGAGGAGA AGGACAAGGA GAAGCCTGCG
    CAGACTGACA CAGAATCTAA GGAAACGCCT GCAAAAGAGG AGATCGCGGA TGACAAATCG
    AAGCAGGATG AGCCTGAGGA AAAGAAAATC GAAAAGCAAT CCAGAGAGGG CGAAGAGAAA
    TCGGAAACGG ACGAAAAGCC CACGGAGATG ACGACCGTGA GCACTGAGGA GGAAAAGCAG
    CCAACAGCCA ATTGTGATAA TCACCAGCAA AACAACAATA GCAACAGCAA AGCCAGCAAC
    GATCAGCAAC CATCCACGTC GAAGGCTGCC CAGAAGCTAC AACTATTTCG TCCATTGTTG
    AACAAGGAGC TCGAGGATTG GATTTGGCAG GACAATCGAC AGTTCCTGCA GGATCGCAAC
    ATTTTCGAGC ACACATATTT CAACTTTATG TGGCAGATAT GTGGGCACAT ACCCCAATCA
    TTAATATCAG AGACGGACGT CACCTGCATG GCAGCCAAGT TGTCTGTGTC CTTTTTCATT
    GAGACTTTTA TTCATGCTAA GGAAAAGCCC ACTATGGTGC CCTGGGTGGA GCTGCTGACC
    AAGCAGTTCA ATGCCAGCCA AGAGGCCTGC GAGTGGTTTC TGTCGCACAT GTCGCAGGAG
    CCCTACTGGC CGGTCCAGGT GCTAATCCAG TGTCCCAACC AAATGGTGCG TCAAATGTTT
    CAGCGTTTGG TAATCCATGT CATCCAGCAA CTACGTGCCT CACATGCTCA TCTGTATCTG
    GAAGTGGAGA CAGACGAGGA TGACAAGGAG TTAATAGGCC AAGCCTCTTG CGTCACCCGT
    TTCATTGGTT CGCTCATCTC GCTCCTGGAG CACGGTGCCC GAGCGAACCT GCGGCACTTG
    TCCGAGTACT TTGGACTGTT GTGCGAGTTC TCACGGATGG GAGATGAGGA AGCCATGTAC
    TTGCTGCGCA TCGGTGTACT CAAGAGCCTG GTCGACTTCT ATCTGGGTCA CAAGCAAACA
    GACAGCATAG ACATAAGCTC TGACAACGAA GACAACTCCT CTGAAGAGGC GCTGTCCGTG
    GAGAAGATGC GTCCGGCCTC GCTGGACAAG ATGATTGCTC TGTGCGCCAG CCTAGTGGAA
    AGATCAAGAG GAGCCGACTT CCGGCTGCGA CTCTCGCCCA AAGACTTCTC TGCAATCGCC
    GGCGGCAAGG GTTTTCCATT TTTGTACCAA CAAATAAAGG ACGGCATTAA TCCGCACCAG
    ACAAAGCATT TAATACACGC GCTTTGTCGC TGGGATGAGC GCCTGGCCAC GCAGATCATT
    GGCATGCTCT TCGCGTCGGT AACCAAGCAC ACGGAGCTGT GCGCGCCATT CTTCAAGCTG
    CTAACTCTGC TCACCGAGAC GCAGGGCGGT CCAGTGGGCC TGCCTTGTTT CACCCAGTTG
    ATCCTGCCAC GAATGTGGGA CGCCGCCGAG TACTGTCCAC AGTCGGTGCT CGACTGGCTC
    TCGCTGCAGG CCACCAAGAA CAAGATCGCC CACGCCTGGA TACTTCAATC GGCCGAGAAG
    TGGCTCGAGC AATTCCTGCT GGCCCACGAT AACACTCGTG TTCGGAACGC TGCCGCCTTT
    CTGCTGGTGG CACTGGTACC CTCCCAGCCA TTCCGGGCCA ACTTCCGCGC CCACTCGCAA
    CACAAGCTGC TGGCCCTCAA TCCGCACAGT TATCGCGACA TAAATAGCGA TGCCCAGGCG
    GTGCTGCACC AGGTGATCAC GCTGCTCCTG CGATTGCTGC GTCCAGCGCG CGTCTATGCG
    GATATCGGAG CCCATGGCAC GACCAAGCTG ACCGCGTACT TTAATCTGCT CAGCTATTGT
    ATGGTTTCAA AGACTGAAAA ACTAATGATA GGTTCGTACA TGCGCTCCCT GTGGGAGCTG
    TTCCATCCAC GCCTCTCGGA GCCCAGTGTT CCGGCCCATC ACAATAAGCA CGCACTATTA
    ACCTTCTGGC ACCACTCACT GGTGGACTGT CCGGAGAATG CTGCCCAGGT GGCCAACTGC
    CCGGAGATTA CGCGCAACAT TGCATTTAAC TATATACTGG CCGATCATGA TGACGCGGAG
    ATAGTCACCT ACAACCGTTC CATGCTGCCC GCCTATTATG GACTACTTCG TCTGTGCTGC
    GAGCAGAGCC GTGCACTCAC ACGCCAATTG TCCCAGCATC AGAATCTTCA GTGGGCATTT
    AAAAACATCA CACCGCATCC CACGCAGTAC GCGGCCGCCG TGGACGAGCT TTTCAAGTTG
    ATGGCTCTGT TCGCCACCCG TCATCCGGAT GCTAGTGAAC AGGAAAAGCT TGATGTGACC
    CAGTTTAGGA GGGCTGTGAT TGTGTCCTAC ACCAGTAGTC TGGATGCACG TGTCTCTTGG
    TCCACGCTGA TCAGTGCTCT TAAGATACTC GTGGACAATG AGGAGGACCG CACGATGGTG
    ATCTTCAACG GCGGCATTGA GATGTGTTTC GAGGCGCTGC ACACGCTACA TTCCATGCAC
    CACGAGGCCA CCGCCTGCCA CGTGGCCGGA GACCTATTCG ATCTCTTGGG CGAGATGCTA
    CTCCTCCTAG CCACTCTTCG AACCCGCACA GACAGTCCTG CTCAAAAGAA GCAGCAGCAA
    CAACAGCAGC AGCTGGAGCA ACAGTTGCAG TTGCAGCAGC AACAGATGCT GCAGAAACAA
    CAGCAGCAGT CGCAAAGCCA AACGCAAACA CCGCAGTCGC CGCAGCAAAA GGAAAAGCAG
    CTGCAGCAAC AAATGCAGCA GCATCTGCAG CTGCAACAGT TGCAGCAAAT GCAGTTCCAG
    CAGCAACACT TCCTGCGCCA GCAACATCAA CACTCGGCGC TGGCAAAGGC ACTGCCGGAT
    GCAGTGAAGC GACTGGCTAC GCTACTCAAC ACCTTCAATT CGCCAGAGAT CAGTCGCATG
    GCCTTGGAGG TGCTCAAGGA GCTGGTGCGC AATCCCAGCC TGGAAACCAT TAGTATTCTG
    GCGCCCATTC TTATCAACTG TCATCTGTCC GTGGCGAATG CTCCCAATGC GATCGGTCCT
    CTGGGACCGT ACTTTCCGCG TCGCGGTGCG AAGCATACGC CTTGGCCATT GGGAGCCAAG
    AACTCACCCC GTCCACCGCG GCCCATGGTA CAGATGTGCA TCGCTCTGGC TGAGCTGACA
    CCACGCGGTC TCGATGCCGA TTACGACGTT CAGGTGGAGT CCTTTTACCG ACCCTACCAT
    GACTTTATCG ATGTCATGAT GCGCATGTGC GTCAACACGG GCACTCTGAA CGACACGCTA
    GTCAAGCTGC AATGCTTGGT GGCCATCGAG TCCACGCCGC TGCACTTCAC CTATTTCCCC
    AAATTCTGGG TGGGCATACA CAACAATGCT CTGACGCACA AATACGTTGA GCTGCTGGTG
    AAAAATCAAC TGTTGGTAGA GTACCTTCAC AATGTCCTTA GGGACGAGCG AAGCATGTTG
    AAGGATGCCT GCGTTCGTGA ATTTCTGGAG CTCTATTATC ACAAGGTTGC CGCCCAGCTG
    CCAGTGGCCC GTATGATCTA CACAATCAAC TATGGTATGC ACTCCAAGGA CGACATTGAT
    GAGCTGTGTG GCGATCTGTT CGCCATCCGA ATCATTGCCC AGGCCACTGG AGTGCCGGCC
    TCAGTGCGCA AGGAGTTGCG AGGATCGCTG AGGGCGCTTC AGAACAAGAG CGAGCGGTTT
    AGGAAGGAAT CGGAGAGGGA GCTATCATCA TCTCCCTATT TGCCAGATCC ATTTCCGAAC
    AAGAAGCAGA AACGTGACTC GCAAAAGATC AAGGAAAAGG AGCACCCTCA GCCGGAGAGC
    GAGAAAGAGA CCTCAACGGA GAACGACAAA CCATCAGATG TTTCCATGGA ATCCAGCGGT
    AACGCGGAGC AAGCAACGGA CAGCACAAAA CCACCAACTC CTGCAGGCAG CGACGATGAG
    CAGATGGACA AAACTTCAGT TCCCTCGTCC GATGACGAGA CAGAGTTGGA GGACGAGCTG
    CAGCCGACGC CCAAGCGCAA CAAGAAGGCT TCGAATAAGA AGACCGCCCA GGATCGTGTC
    AACGAGGAGC GCGAGAAGCG ACTTATCACG CTGATCACCA TGGAATCATA TATCCAGAGC
    ATTTTTGCCA TCCTTAAGAG AGATGCCAGT GTCCCCAGTT CGGGAGCGAC TAAGGAGCCC
    AGTGAGGAAC CGTGTGGCGA GGCATCCACT TCGGCGGCTG CCGCTGTAAA GTTATCTCGT
    CCCAAAGGAG CCTGTACTCC GCCGGAGCCA ATTACCGACG TAAACGATAC GCGTTGCAAC
    ATAGATACTG AGACAGAGGC GGAGGCGGAT GAGCAATCTC CAAAGACGAG CCAGACAAAT
    GGTAGCCAAC AGAACGAATC CCCGCCAGCG GCGACCAGTG CAGACACTGC ACCCGCGAAT
    CCTTCACCAG CTCCCGCGGC CGCCGTTGCG TCCACATCCC AGGCGGCAAG TCCAACACAG
    ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001287508.1
    CDS358..12123
    Translation

    Target ORF information:

    RefSeq Version NM_001300579.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster puffyeye (puf), transcript variant I, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001300579.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    ATGTGCGAGG TGTGCGCCGA CTTCCAGAAT CTCCTGGAGC TCTATGAGGT GCGCGTGGCC 
    AGCTCGGATC TGAAGTTCCA GCTGCTGCTG AAGAGCGAGA TCGAGACCAC CTTCAACTAC
    ATCCAGTCGT GGCCGCAGCG TCAGTGCATG TGCCTGTACC GGGACACCAA GAACTATGAC
    CGTTTCAACC TGGTAGTGCA GTCGCTCATC TGTCTGACGG TGCAGCATCT GAAGCACATC
    GACCACCTCA TCGACAACTA CAAGCGGCTA ACGGCCAGTG CTGCCCAGGT GGTGGCCCAG
    GCCCAGCAGC AGCGTGAACA GCAGCGGGAC GAAGCGTCAG CCCAGGCAGA GGCAAAGGAA
    TCATCCGCGC CAGCGGAAGA ACCTAAGAAG GAGGAGCCAT CTGGGTCCGC CGGTGAGGAG
    GCTCAGGGCT CCGGCGACGG GCCAGCCAAA AAGCCGCCAG TTGGCCCCTG CACTCCCCCT
    CCCCCGCAAA CGGCCAATCC TCAGCACAAA TCCCACCTGC AGTACACAGA GGAGCCCTGG
    ATCCTGCCCG AGGTGGAGAA ACTGTTGGTG CTCGTTTCAA AGGTGTTCCT GCTCAACTTC
    CCGCTGTACA TCGCCCACAA ACATGGCATG CACTCTCGGC TGGACGACCT GCAGGCTGAG
    GAGGCCCACC ACCTGGCCCT CATCTGCGAT CTTCACGACA ACGACCTGCC CATCTATCTG
    CTGCGGAATG TGAGCCTGTT CTGCAACTCG GGCGGCTTTG GCGCCATGTC GCTGTGCTTC
    GAACATCCCG ATCTGCCAGT GTCCACCGCT CACTCGATGA CCGCTGCCGT CTCAAATGTG
    AAACTTTGGC TGAACTATCA CTGTAATACT CAGCTCTTCG TGCCGCTGCG AAGTCGGATA
    CTGCAGTACA TGTGCAAGCT GTCGGATCAG AGCCTCCGAT CTGCCGCAAC CCGCGCGATG
    GCCGATTTTG TGTGGTCCTC TATGCGAGAC CCACTGGATG TTGCTGTCAA CTTCGACACC
    GAAGGATTGG CCCTGGCCTT TAAGTACTTC ACATCGACCA CGCTAACCAT GCGACTGGCA
    GGCATGGCCC AGATCAACGC ACACATTAAT CTATTTAACG AGATCTGCAC CACCGAAACG
    GTCAACGAAG TGGAGCTGTT CGGGCAGCGC ATCGCGAACT GGCTGACTGA AAATCATATT
    GTACAGCACC TGTTTGGCCC GAATCTGCAC GTAGAAATCG TTAAACAGGC ACACGTGCTG
    CTCAACTTTT TGGCGGTAGA GAACCAGATC TCTGAAGAGG ACATCAAGCT GATCTGGCAA
    GCCACCCAGC TGAAGCACTG CTCAAAGACG ATCTTCGACA TCCTGCCTTC GCTGGTGAAG
    AATCTGACCC CGCGCCCGGC AATGCACCTT TACTCCCTCC TTTGCCGCAT GGATCCCAAG
    GAGCACACTG AGCAGAGCAT ATACATAGCC TCAGCGCTAA CAAAACAGCT GTGGACTCGT
    GATACCTCAC GCAGTCAGAT GAACCTGATG CAAGACCACC TGCTGGGGTC CAACGTCACC
    GCTTCCAGCT CTGACAGCGG CAGCATTGAG GGCAGCAACA CAGAGGACGA CCACGTGGGC
    GCCGACGACA GTAGCATTGC CAGCGGTGGC GGCGGCGGAG GTGTTGGTAA CAAGAGTCCA
    ATCGATGGAG TTACTCCATG CAAGCAGGCC CGGCACAGGC GGCACATCTG TGACCCCACC
    ACCGAGAAGG GAAAGCAAAT AAGTCCCGAG GATATGGCCA AAGTAGACTT GGTGAACAAA
    CGGATTGTGA ACATTATCGA CAACACAAGC AGTGAAGAGG AGCTGCAATC GCGGGCCGCA
    CTGGAGCTGC AACTGCATCG CAGCCGCAAA AAGACGAGCA ACAAGCGACG GCGACAAAAA
    ACCAACAAAC AGATCATTCT GCCCCATGAG CTGGTCGAGA TTTGGGACGG AGTTGAAGAT
    GTGCCCAGCA GTGACGAGGC TGATGGCGAA GCCGATGGTG ATGGCGAGGG CGAACTTTTA
    GCGGACAGCG ATGAGTGCAG CGATGGTGCT ACGAGTCAGC TGGTACCCGA CGCCGTGCTA
    AAGCATCTGC AGGGAGAGGG ACCTTTTATC AGGGCCATCG AGACCAATAT CAATGAGCTG
    CTCAGTGGTG CCGAGAACGA TGGCAGTTAC TCATCCCCGA TGAGTAACAA ATCTGAAAAG
    AACCTGGCTG ACTTCGATGA CGAGGACGTG TCGCCTTGCG AGGAGGAGCT GGCCCAGCTG
    GTCAGCTCCC GCGCCAATTG TTCGGACGTT CCACCAGCCT TTGCAGCCGC TGCCGCCGCC
    ATGATGGTGG CCCAGTCGGC GATGGCCCTT CAGAAGTCTG GAGAGTCGAA TGTGGCTGCT
    GCTGCCGCTG CTGTGGCCGC TGCTGCAGCG GCTACCGGAA CTGCACAGCA AACGCTGGTG
    GCAATGAACC GGCAGGCCAG CGTTGCTGCA GCTGCCGCCG TGGTTGCTGC GGCCAAGGCA
    AAATCGGACT CAGATGTCGA TCTGATGGAT GTGGTTTCCG GCGGAAAGCA GCACCATTCA
    CCAAAGGCGA GCCAGGGCAG CTCTACGTCC GGGAGTACAC CGGTGCAGCC CTCGTTCAAG
    CTAAATGATG TCTGCCAGCC CGGAAACACA CTGCTTTGGG ATCTGCTTCA AGACGATAAA
    ATTGGCCAAC TAGGCGAGTC TCTGGCTCTA GAGGCGGAGA AAGCTCTGGC CACTCTCCTG
    TGCTTCAGCA TGGACCGCCA ACTGCGAACG AAATTCATCG AGGGTTGTCT GTATAACGTC
    GCCAACAACC GGAGTGTTAT TGTCAGTCTG CGACTGCTGC CCAAACTGTT CGCCTCGTTC
    CAACAGTTTC GACCCTCGGA CACGCACTCT ATGACGATGT GGGCAGAGCG AAATCACCGG
    ATGATGCAGT GCTTTTTCAA TAATATACGT CACTATGCGC GTCGCCATGC CGAGGTACTG
    ATCACCCAAA ACGGAGAGCA GCAGCAGCAG CAGCTGGGCG GACAACTCTA CTCGCACAAG
    ACACAAGTTT CTGTAAGACT GCAGTTCCTA TCATCGATAT TCTCGACGGT GGGATCCCCG
    AAAAGCTTCC GCCTAACGCT GGAGCAGCTT GACGCGCTAT GGGAGTGGCT GGCCCACGAT
    CCGGAGTGTG CCGACTGCTA CTTTTCGTGG CTGCAGGCCC AGGCCAAAGG CGGTGATCAG
    CATGCTCTGG GCATTGAAGC ACTTCAGCAT TTGTATTTGA AGAAACTCCC AGAGCTGCGA
    CCCGAGGAGT TCTCTATGGT TGCGTTGGGA CTATTTCAAC AGCTCTGTAG CTTCGCCCGC
    ATCGCCATGG CCGAGTACGA CAATCATAGC GACCAGATCT CGGCGAGCGC CAGTGCCGTT
    GGCATGTACC ATCTTTGGAA GATAGCGTTG CGTGCCCAGA GCAACGATGT CTCCTTGGCG
    GCCATACAGT ACATCAACAT GTACTACATG GGTCAACAAT TGCGCCTGGA GAAGGAGTTC
    GTTTCACAGT GTATGGAAAA CCTAGTTCAA GCGGCCACGG CTTTGGAAAG CATCGATGAT
    GAGAATGCTT TGATGCGTGT CCAGCGTGGC CTGCTCTTGC TGAACACCCA TTTGGATACG
    TTCCGGCGTC GATATGCGTT TCATCTGCGC CGCTGGGCCA TCGAAGGCAA AGGGATTGGA
    TCGCACAGTA ACCTTAAAAA TGAGGGAGCG GGTCCACCAC TACGAATAGT GCTGCAACAG
    GCAGGACTTT CGGAAAAAAG CCTTTTGCAG ATGCACGCGT GTGACTTAAT CGCCGACCTC
    AAGGCTGAGG TGTCCAAGTG GTGGGAGAGC CTTCAAACTG GACTAGCTGC TCCTGTGCTC
    GGTCTGCTGC TCAGCGATGG ACCGCTACGC ATTATCACAC AGGGACAGGA ATTGACCTCG
    GATTACGATG AACGGAGTCT CGGAGATGCT GGTTTCAAGG ATAACCAGAT CGTCTACGTA
    TCGTTAGGTG GACGTGGAGC CAGGCGAAAG GAATCCAATT TGGAGCATCC GTCAATGCTG
    CCGCCCCCAC CAAAGGAGTG TTTGCCCACG GTGCTCCTGC TGCAGCCAAA GTACTTCGAG
    AAGCTATTTT GCCTGATGCA GACCCTGGGC GATATGCAAC CGCAAGCGTC GACTGTAAAT
    CCCCAGCACC ACACAAAGGC GCAATTGCTC TCGCGACGCG TATGGGATAT CCTCGCCATG
    CTGCCAACTA ATCCGCACAT TCTAGACGCC TTTAAGAGTC TGGTTACGGA CCTAAGCGAA
    CTCGAGCAGC TTGATGCTGG CGGAGAGGAG GAGCAACTGG CCACCAAGCG CAAGCAAATC
    AAGCAGAAGT TCCGAGATCT TCTCGACCCG AACAACTTGC AAAAGTTCAT GTACTCCCTC
    CATATTGTGG AGAGTCTGGC CCTAACAAGT TCCAGACGTG GTGAATCCAA CGGCAATGTG
    GCCATGGGAA ATACACCGGA ACAGGTACGG GTGAAGAAAT CCTCGATGGG AAGACGGAGA
    AATAGCAACG AGCAGCCGCC ACCGCCGCCG CCAGAAGTCA AGATGAGCAA GGAGCAACTG
    TGCGAGCTGG AGGCCCCACT GACGCCCACA CCCTCGACTG GCTTGCAGGA TGTGGAAACG
    GAGGCCAGCA GTAGCAGCGG TGGCGACAAA GAAAACCAAC CGAAACAACA CAGCAAGCGT
    CAGAAGAAAG GCGAAACTTT TGAACAGGAA AAGGAGCGCC CAGTGGGTTG CTCCACGCCG
    CCGTCTCCAA CTCCTCCGCC ACCAGCACTT TCGGTAGTGG AAAGAGGTGA CAATAAGTGG
    AGTGAGGCGT TCGTCAAATG TGGTGGCCTT CGTCATCTGT ACGAAATCTT TAGTGAGGGC
    CAACTACAAC AAAGCGCACA TCCCAAGGAG TTGGCTTTAA ATGAATGGCG ACACGATTGC
    CTGGCCAGCC TGCTGCGCAT TCTCTGGCTT CTTGGATTCG AGGAACTGCA GTCGGCGGAT
    GCCCATGTTC TAATGTCACG TCCGCACCCG TTTATGCTAC AGTTGATGGA GGTACCACAG
    TGCCTGACCC GACTCTCATC GATCCTTAAC GACGAAGTTC ACCAGCAGCA TCAGGCTTCT
    TCGGCCAATC CACTGGTATT CCCCTATCAG TTCCAGCACT TGCGTACTGG CTTTTGGGGC
    AGGGCCCAGC TCATCCAGTT CGCTATGAAC ATTCTAGTGA GTTTTGTGCA CGCCTCAGCG
    GAAGCGAGAA GGCTTCTCTG GGCCCCAACT GGTACCGATC ACTGTCGATG GCTGCAGAAG
    TTCATCCTGG AGGATCCCGA ACCGGCGGTA CGGCGTGAGA TATGCGCTGG TCTTTATCGC
    ATTTGCTTAG GAAACGCACA TAGCTATCGC CTCTTACTAG CACCACTGCT GCACAAGCTC
    ATCGCCCTGT TGCCATTGGC CGAGCAGATG AGCTCTGGCA ACCAGCACAC GCAGTTTTTG
    CTCTCGGAAG AGGGCAAGGA TCCCTACGGA CCCGCCTGCC GTGACTACTT TTGGCTGCTG
    GCCAGATTGG TGGATACTCT CTCCCCGGAA ATGGTGGCCG AGGAGCACAT CGACATTGAA
    ATGCTGTGCG AAAGCATCTC GCAGAGCATT CTCACCCGAG AGTATTATGA ACTGCGCCAT
    GGTTACCAGG ACGATGGCTT GGTGGGTCTC CTAAATCTCA TGTCCAATTT GATCAAGTAC
    GACACCACAT TCAAGTACAC ACCCAAGGCG TTATCCTTTA TTGAGCAACT GATTGGCTTC
    CTGTTTGACA TGCCCTCGCC GGCAGATCGC CAGAAGCCCA AGTGCAAGTC GGCGTCGTCT
    CGAGCATCTG CCTACGATCT GCTGGTTGAA CTTTGCCGTG GATGCGCCAC AAACTATGCA
    TATCTGCATG GACGTCTGTT GGCACAGCAC AAATCCGGAC CCAAGCAGCC GTATCCCTGG
    GACTATTGGC CGCGCGATGA AGGCAGAGCT GAGTGCGGAT ATGTGGGGCT AACTAATTTG
    GGAGCCACTT GCTATATGGC CTCTTGCGTC CAGCACTTGT ACATGATGCC ACAGGCGCGA
    GCAGCTGTTT TGCGAGTTCC ACCCAACGCC GCCCGTAAGC ATGGGCCCAC ACTTCTGGAA
    CTACAACGGA TGTTTGCCTA CCTACTGGAG TCGGAACGGA AGTCTTACAA CCCGCGAAGC
    TTCTGCCGGG TGTACCAGAT GGATCACCAG CCGCTAAATA CGGGAGAGCA AAAGGATATG
    GCAGAGTTCT TTATCGATTT GGTGTCCAAA CTGGAAGATA TGACGCCTGA CTTGAAGCAC
    CTGGTGAAAA GGCTCTTCTG TGGATCCTTG AGCAATAATG TCGTCTCCCT GGATTGCGGT
    CACGTTTCCC GCACGGCCGA GGACTTTTAC ACGGTTCGCT GCCAGGTTGC AGATATGCGC
    AATCTGCAGG AATCCCTGGA CGAAGTCACC GTTAAGGATA CCCTCGAGGG CGACAACATG
    TACACCTGCT CGCAGTGCGG CAAAAAAGTA CGAGCCGAGA AGCGGGCTTG CTTCAAGAAG
    CTTCCCCAGA TCTTGTGCTT CAACACCATG CGCTACACCT TCAATATGGT CACCATGCTC
    AAGGAGAAGG TCAACACGCA CTTTTCCTTT CCGCTACGCC TGAACATGTG TCACTATGTG
    GAGAAGACCT TGATGCCGCA GCAGTACAAA GAGGAGCGAG AAAGGCGCCA AAAGGAGAAG
    GAAGGAGCAG ATGGTAGTGG CGATGGGAAT GACAACGAAA AGGCAGAGGC CACGCTAGAT
    GACGATATTG AAGAATGCTA CGAGTACGAA CTGGTGGGCG TCACTGTCCA CACGGGCACA
    GCGGATGGCG GCCACTACTA CAGCTTTATA AAGGAGCGGA CGAAGACTAG TTACCATACG
    CACGAGCGGT GGTTTCTTTT CAACGATGCC GAAGTGAAGC CTTTCGATCC CTCACAGATA
    GCGGCGGAGT GCTTTGGCGG CGAGATGACG AGCAAAACCT ACGACTCAGT GACGGAGAAA
    TATCTGGACT TTAGTTTCGA GAAGACCAAC TCTGCCTACA TGCTGTTCTA CGAGCGCCGA
    CTGCCAGAGC ATCTGCAGCG CCGGCATTCC GAGCTGCTGG TTACACCCAC GCCCAGTCCC
    ACAGTGGAAG AGAAGTCCGA GGCGGAGGAG CCCACTAAAA TGGAGACAAG CTCTAGCGAA
    ATCAAGGCAG ATGTGGATGT GGAAGTGGAA GTTGAGGAGA AGGACAAGGA GAAGCCTGCG
    CAGACTGACA CAGAATCTAA GGAAACGCCT GCAAAAGAGG AGATCGCGGA TGACAAATCG
    AAGCAGGATG AGCCTGAGGA AAAGAAAATC GAAAAGCAAT CCAGAGAGGG CGAAGAGAAA
    TCGGAAACGG ACGAAAAGCC CACGGAGATG ACGACCGTGA GCACTGAGGA GGAAAAGCAG
    CCAACAGCCA ATTGTGATAA TCACCAGCAA AACAACAATA GCAACAGCAA AGCCAGCAAC
    GATCAGCAAC CATCCACGTC GAAGGCTGCC CAGAAGCTAC AACTATTTCG TCCATTGTTG
    AACAAGGAGC TCGAGGATTG GATTTGGCAG GACAATCGAC AGTTCCTGCA GGATCGCAAC
    ATTTTCGAGC ACACATATTT CAACTTTATG TGGCAGATAT GTGGGCACAT ACCCCAATCA
    TTAATATCAG AGACGGACGT CACCTGCATG GCAGCCAAGT TGTCTGTGTC CTTTTTCATT
    GAGACTTTTA TTCATGCTAA GGAAAAGCCC ACTATGGTGC CCTGGGTGGA GCTGCTGACC
    AAGCAGTTCA ATGCCAGCCA AGAGGCCTGC GAGTGGTTTC TGTCGCACAT GTCGCAGGAG
    CCCTACTGGC CGGTCCAGGT GCTAATCCAG TGTCCCAACC AAATGGTGCG TCAAATGTTT
    CAGCGTTTGG TAATCCATGT CATCCAGCAA CTACGTGCCT CACATGCTCA TCTGTATCTG
    GAAGTGGAGA CAGACGAGGA TGACAAGGAG TTAATAGGCC AAGCCTCTTG CGTCACCCGT
    TTCATTGGTT CGCTCATCTC GCTCCTGGAG CACGGTGCCC GAGCGAACCT GCGGCACTTG
    TCCGAGTACT TTGGACTGTT GTGCGAGTTC TCACGGATGG GAGATGAGGA AGCCATGTAC
    TTGCTGCGCA TCGGTGTACT CAAGAGCCTG GTCGACTTCT ATCTGGGTCA CAAGCAAACA
    GACAGCATAG ACATAAGCTC TGACAACGAA GACAACTCCT CTGAAGAGGC GCTGTCCGTG
    GAGAAGATGC GTCCGGCCTC GCTGGACAAG ATGATTGCTC TGTGCGCCAG CCTAGTGGAA
    AGATCAAGAG GAGCCGACTT CCGGCTGCGA CTCTCGCCCA AAGACTTCTC TGCAATCGCC
    GGCGGCAAGG GTTTTCCATT TTTGTACCAA CAAATAAAGG ACGGCATTAA TCCGCACCAG
    ACAAAGCATT TAATACACGC GCTTTGTCGC TGGGATGAGC GCCTGGCCAC GCAGATCATT
    GGCATGCTCT TCGCGTCGGT AACCAAGCAC ACGGAGCTGT GCGCGCCATT CTTCAAGCTG
    CTAACTCTGC TCACCGAGAC GCAGGGCGGT CCAGTGGGCC TGCCTTGTTT CACCCAGTTG
    ATCCTGCCAC GAATGTGGGA CGCCGCCGAG TACTGTCCAC AGTCGGTGCT CGACTGGCTC
    TCGCTGCAGG CCACCAAGAA CAAGATCGCC CACGCCTGGA TACTTCAATC GGCCGAGAAG
    TGGCTCGAGC AATTCCTGCT GGCCCACGAT AACACTCGTG TTCGGAACGC TGCCGCCTTT
    CTGCTGGTGG CACTGGTACC CTCCCAGCCA TTCCGGGCCA ACTTCCGCGC CCACTCGCAA
    CACAAGCTGC TGGCCCTCAA TCCGCACAGT TATCGCGACA TAAATAGCGA TGCCCAGGCG
    GTGCTGCACC AGGTGATCAC GCTGCTCCTG CGATTGCTGC GTCCAGCGCG CGTCTATGCG
    GATATCGGAG CCCATGGCAC GACCAAGCTG ACCGCGTACT TTAATCTGCT CAGCTATTGT
    ATGGTTTCAA AGACTGAAAA ACTAATGATA GGTTCGTACA TGCGCTCCCT GTGGGAGCTG
    TTCCATCCAC GCCTCTCGGA GCCCAGTGTT CCGGCCCATC ACAATAAGCA CGCACTATTA
    ACCTTCTGGC ACCACTCACT GGTGGACTGT CCGGAGAATG CTGCCCAGGT GGCCAACTGC
    CCGGAGATTA CGCGCAACAT TGCATTTAAC TATATACTGG CCGATCATGA TGACGCGGAG
    ATAGTCACCT ACAACCGTTC CATGCTGCCC GCCTATTATG GACTACTTCG TCTGTGCTGC
    GAGCAGAGCC GTGCACTCAC ACGCCAATTG TCCCAGCATC AGAATCTTCA GTGGGCATTT
    AAAAACATCA CACCGCATCC CACGCAGTAC GCGGCCGCCG TGGACGAGCT TTTCAAGTTG
    ATGGCTCTGT TCGCCACCCG TCATCCGGAT GCTAGTGAAC AGGAAAAGCT TGATGTGACC
    CAGTTTAGGA GGGCTGTGAT TGTGTCCTAC ACCAGTAGTC TGGATGCACG TGTCTCTTGG
    TCCACGCTGA TCAGTGCTCT TAAGATACTC GTGGACAATG AGGAGGACCG CACGATGGTG
    ATCTTCAACG GCGGCATTGA GATGTGTTTC GAGGCGCTGC ACACGCTACA TTCCATGCAC
    CACGAGGCCA CCGCCTGCCA CGTGGCCGGA GACCTATTCG ATCTCTTGGG CGAGATGCTA
    CTCCTCCTAG CCACTCTTCG AACCCGCACA GACAGTCCTG CTCAAAAGAA GCAGCAGCAA
    CAACAGCAGC AGCTGGAGCA ACAGTTGCAG TTGCAGCAGC AACAGATGCT GCAGAAACAA
    CAGCAGCAGT CGCAAAGCCA AACGCAAACA CCGCAGTCGC CGCAGCAAAA GGAAAAGCAG
    CTGCAGCAAC AAATGCAGCA GCATCTGCAG CTGCAACAGT TGCAGCAAAT GCAGTTCCAG
    CAGCAACACT TCCTGCGCCA GCAACATCAA CACTCGGCGC TGGCAAAGGC ACTGCCGGAT
    GCAGTGAAGC GACTGGCTAC GCTACTCAAC ACCTTCAATT CGCCAGAGAT CAGTCGCATG
    GCCTTGGAGG TGCTCAAGGA GCTGGTGCGC AATCCCAGCC TGGAAACCAT TAGTATTCTG
    GCGCCCATTC TTATCAACTG TCATCTGTCC GTGGCGAATG CTCCCAATGC GATCGGTCCT
    CTGGGACCGT ACTTTCCGCG TCGCGGTGCG AAGCATACGC CTTGGCCATT GGGAGCCAAG
    AACTCACCCC GTCCACCGCG GCCCATGGTA CAGATGTGCA TCGCTCTGGC TGAGCTGACA
    CCACGCGGTC TCGATGCCGA TTACGACGTT CAGGTGGAGT CCTTTTACCG ACCCTACCAT
    GACTTTATCG ATGTCATGAT GCGCATGTGC GTCAACACGG GCACTCTGAA CGACACGCTA
    GTCAAGCTGC AATGCTTGGT GGCCATCGAG TCCACGCCGC TGCACTTCAC CTATTTCCCC
    AAATTCTGGG TGGGCATACA CAACAATGCT CTGACGCACA AATACGTTGA GCTGCTGGTG
    AAAAATCAAC TGTTGGTAGA GTACCTTCAC AATGTCCTTA GGGACGAGCG AAGCATGTTG
    AAGGATGCCT GCGTTCGTGA ATTTCTGGAG CTCTATTATC ACAAGGTTGC CGCCCAGCTG
    CCAGTGGCCC GTATGATCTA CACAATCAAC TATGGTATGC ACTCCAAGGA CGACATTGAT
    GAGCTGTGTG GCGATCTGTT CGCCATCCGA ATCATTGCCC AGGCCACTGG AGTGCCGGCC
    TCAGTGCGCA AGGAGTTGCG AGGATCGCTG AGGGCGCTTC AGAACAAGAG CGAGCGGTTT
    AGGAAGGAAT CGGAGAGGGA GCTATCATCA TCTCCCTATT TGCCAGATCC ATTTCCGAAC
    AAGAAGCAGA AACGTGACTC GCAAAAGATC AAGGAAAAGG AGCACCCTCA GCCGGAGAGC
    GAGAAAGAGA CCTCAACGGA GAACGACAAA CCATCAGATG TTTCCATGGA ATCCAGCGGT
    AACGCGGAGC AAGCAACGGA CAGCACAAAA CCACCAACTC CTGCAGGCAG CGACGATGAG
    CAGATGGACA AAACTTCAGT TCCCTCGTCC GATGACGAGA CAGAGTTGGA GGACGAGCTG
    CAGCCGACGC CCAAGCGCAA CAAGAAGGCT TCGAATAAGA AGACCGCCCA GGATCGTGTC
    AACGAGGAGC GCGAGAAGCG ACTTATCACG CTGATCACCA TGGAATCATA TATCCAGAGC
    ATTTTTGCCA TCCTTAAGAG AGATGCCAGT GTCCCCAGTT CGGGAGCGAC TAAGGAGCCC
    AGTGAGGAAC CGTGTGGCGA GGCATCCACT TCGGCGGCTG CCGCTGTAAA GTTATCTCGT
    CCCAAAGGAG CCTGTACTCC GCCGGAGCCA ATTACCGACG TAAACGATAC GCGTTGCAAC
    ATAGATACTG AGACAGAGGC GGAGGCGGAT GAGCAATCTC CAAAGACGAG CCAGACAAAT
    GGTAGCCAAC AGAACGAATC CCCGCCAGCG GCGACCAGTG CAGACACTGC ACCCGCGAAT
    CCTTCACCAG CTCCCGCGGC CGCCGTTGCG TCCACATCCC AGGCGGCAAG TCCAACACAG
    ATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.