puf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	puf
Entrez Gene ID	42935
Full Name	puffyeye
Synonyms	BcDNA:AT28817,CG5794,Dmel\CG5794,NEST:bs19d10,Q9VC56,USP34,Usp34
General protein information	Preferred Names puffyeye Names CG5794 gene product from transcript CG5794-RG CG5794-PD CG5794-PE CG5794-PF CG5794-PG CG5794-PH CG5794-PI puf-PD puf-PE puf-PF puf-PG puf-PH puf-PI
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96A13-96A13

mRNA and Protein(s)

mRNA	Protein	Name
NM_206564.2	NP_996287.1	puffyeye, isoform E
NM_170158.3	NP_733022.2	puffyeye, isoform F
NM_001300579.1	NP_001287508.1	puffyeye, isoform I
NM_001275993.2	NP_001262922.1	puffyeye, isoform G
NM_143018.4	NP_651275.1	puffyeye, isoform D
NM_001275994.2	NP_001262923.1	puffyeye, isoform H

Pathways from BioSystems

Homologies

Homo sapiens (human)	USP34	NP_055524.3
Canis lupus familiaris (dog)	USP34	XP_005626192.1
Rattus norvegicus (Norway rat)	Usp34	NP_001258125.1
Danio rerio (zebrafish)	usp34	XP_002660609.3
Bos taurus (cattle)	USP34	XP_002691468.3
Mus musculus (house mouse)	Usp34	NP_001177330.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003643	XP_313408.5
Pan troglodytes (chimpanzee)	USP34	XP_003309103.1
	USP34	XP_002799306.1
Gallus gallus (chicken)	USP34	NP_001186345.1
Drosophila melanogaster (fruit fly)	CG5794	NP_001262922.1
Xenopus tropicalis (tropical clawed frog)	usp34	XP_002939554.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following puf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the puf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05977	NM_170158.3 Latest version!	Drosophila melanogaster puffyeye (puf), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa05978	NM_143018.4 Latest version!	Drosophila melanogaster puffyeye (puf), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa05979	NM_206564.2 Latest version!	Drosophila melanogaster puffyeye (puf), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa07738	NM_001275994.2 Latest version!	Drosophila melanogaster puffyeye (puf), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa07739	NM_001275993.2 Latest version!	Drosophila melanogaster puffyeye (puf), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa22151	NM_001300579.1 Latest version!	Drosophila melanogaster puffyeye (puf), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

puf ( NM_170158.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733022.2 Drosophila melanogaster puffyeye (puf), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa05977
Clone ID Related Accession (Same CDS sequence)	NM_170158.3
Accession Version	NM_170158.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	puffyeye, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_170158.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCGAGG TGTGCGCCGA CTTCCAGAAT CTCCTGGAGC TCTATGAGGT GCGCGTGGCC 
AGCTCGGATC TGAAGTTCCA GCTGCTGCTG AAGAGCGAGA TCGAGACCAC CTTCAACTAC 
ATCCAGTCGT GGCCGCAGCG TCAGTGCATG TGCCTGTACC GGGACACCAA GAACTATGAC 
CGTTTCAACC TGGTAGTGCA GTCGCTCATC TGTCTGACGG TGCAGCATCT GAAGCACATC 
GACCACCTCA TCGACAACTA CAAGCGGCTA ACGGCCAGTG CTGCCCAGGT GGTGGCCCAG 
GCCCAGCAGC AGCGTGAACA GCAGCGGGAC GAAGCGTCAG CCCAGGCAGA GGCAAAGGAA 
TCATCCGCGC CAGCGGAAGA ACCTAAGAAG GAGGAGCCAT CTGGGTCCGC CGGTGAGGAG 
GCTCAGGGCT CCGGCGACGG GCCAGCCAAA AAGCCGCCAG TTGGCCCCTG CACTCCCCCT 
CCCCCGCAAA CGGCCAATCC TCAGCACAAA TCCCACCTGC AGTACACAGA GGAGCCCTGG 
ATCCTGCCCG AGGTGGAGAA ACTGTTGGTG CTCGTTTCAA AGGTGTTCCT GCTCAACTTC 
CCGCTGTACA TCGCCCACAA ACATGGCATG CACTCTCGGC TGGACGACCT GCAGGCTGAG 
GAGGCCCACC ACCTGGCCCT CATCTGCGAT CTTCACGACA ACGACCTGCC CATCTATCTG 
CTGCGGAATG TGAGCCTGTT CTGCAACTCG GGCGGCTTTG GCGCCATGTC GCTGTGCTTC 
GAACATCCCG ATCTGCCAGT GTCCACCGCT CACTCGATGA CCGCTGCCGT CTCAAATGTG 
AAACTTTGGC TGAACTATCA CTGTAATACT CAGCTCTTCG TGCCGCTGCG AAGTCGGATA 
CTGCAGTACA TGTGCAAGCT GTCGGATCAG AGCCTCCGAT CTGCCGCAAC CCGCGCGATG 
GCCGATTTTG TGTGGTCCTC TATGCGAGAC CCACTGGATG TTGCTGTCAA CTTCGACACC 
GAAGGATTGG CCCTGGCCTT TAAGTACTTC ACATCGACCA CGCTAACCAT GCGACTGGCA 
GGCATGGCCC AGATCAACGC ACACATTAAT CTATTTAACG AGATCTGCAC CACCGAAACG 
GTCAACGAAG TGGAGCTGTT CGGGCAGCGC ATCGCGAACT GGCTGACTGA AAATCATATT 
GTACAGCACC TGTTTGGCCC GAATCTGCAC GTAGAAATCG TTAAACAGGC ACACGTGCTG 
CTCAACTTTT TGGCGGTAGA GAACCAGATC TCTGAAGAGG ACATCAAGCT GATCTGGCAA 
GCCACCCAGC TGAAGCACTG CTCAAAGACG ATCTTCGACA TCCTGCCTTC GCTGGTGAAG 
AATCTGACCC CGCGCCCGGC AATGCACCTT TACTCCCTCC TTTGCCGCAT GGATCCCAAG 
GAGCACACTG AGCAGAGCAT ATACATAGCC TCAGCGCTAA CAAAACAGCT GTGGACTCGT 
GATACCTCAC GCAGTCAGAT GAACCTGATG CAAGACCACC TGCTGGGGTC CAACGTCACC 
GCTTCCAGCT CTGACAGCGG CAGCATTGAG GGCAGCAACA CAGAGGACGA CCACGTGGGC 
GCCGACGACA GTAGCATTGC CAGCGGTGGC GGCGGCGGAG GTGTTGGTAA CAAGAGTCCA 
ATCGATGGAG TTACTCCATG CAAGCAGGCC CGGCACAGGC GGCACATCTG TGACCCCACC 
ACCGAGAAGG GAAAGCAAAT AAGTCCCGAG GATATGGCCA AAGTAGACTT GGTGAACAAA 
CGGATTGTGA ACATTATCGA CAACACAAGC AGTGAAGAGG AGCTGCAATC GCGGGCCGCA 
CTGGAGCTGC AACTGCATCG CAGCCGCAAA AAGACGAGCA ACAAGCGACG GCGACAAAAA 
ACCAACAAAC AGATCATTCT GCCCCATGAG CTGGTCGAGA TTTGGGACGG AGTTGAAGAT 
GTGCCCAGCA GTGACGAGGC TGATGGCGAA GCCGATGGTG ATGGCGAGGG CGAACTTTTA 
GCGGACAGCG ATGAGTGCAG CGATGGTGCT ACGAGTCAGC TGGTACCCGA CGCCGTGCTA 
AAGCATCTGC AGGGAGAGGG ACCTTTTATC AGGGCCATCG AGACCAATAT CAATGAGCTG 
CTCAGTGGTG CCGAGAACGA TGGCAGTTAC TCATCCCCGA TGAGTAACAA ATCTGAAAAG 
AACCTGGCTG ACTTCGATGA CGAGGACGTG TCGCCTTGCG AGGAGGAGCT GGCCCAGCTG 
GTCAGCTCCC GCGCCAATTG TTCGGACGTT CCACCAGCCT TTGCAGCCGC TGCCGCCGCC 
ATGATGGTGG CCCAGTCGGC GATGGCCCTT CAGAAGTCTG GAGAGTCGAA TGTGGCTGCT 
GCTGCCGCTG CTGTGGCCGC TGCTGCAGCG GCTACCGGAA CTGCACAGCA AACGCTGGTG 
GCAATGAACC GGCAGGCCAG CGTTGCTGCA GCTGCCGCCG TGGTTGCTGC GGCCAAGGCA 
AAATCGGACT CAGATGTCGA TCTGATGGAT GTGGTTTCCG GCGGAAAGCA GCACCATTCA 
CCAAAGGCGA GCCAGGGCAG CTCTACGTCC GGGAGTACAC CGGTGCAGCC CTCGTTCAAG 
CTAAATGATG TCTGCCAGCC CGGAAACACA CTGCTTTGGG ATCTGCTTCA AGACGATAAA 
ATTGGCCAAC TAGGCGAGTC TCTGGCTCTA GAGGCGGAGA AAGCTCTGGC CACTCTCCTG 
TGCTTCAGCA TGGACCGCCA ACTGCGAACG AAATTCATCG AGGGTTGTCT GTATAACGTC 
GCCAACAACC GGAGTGTTAT TGTCAGTCTG CGACTGCTGC CCAAACTGTT CGCCTCGTTC 
CAACAGTTTC GACCCTCGGA CACGCACTCT ATGACGATGT GGGCAGAGCG AAATCACCGG 
ATGATGCAGT GCTTTTTCAA TAATATACGT CACTATGCGC GTCGCCATGC CGAGGTACTG 
ATCACCCAAA ACGGAGAGCA GCAGCAGCAG CAGCTGGGCG GACAACTCTA CTCGCACAAG 
ACACAAGTTT CTGTAAGACT GCAGTTCCTA TCATCGATAT TCTCGACGGT GGGATCCCCG 
AAAAGCTTCC GCCTAACGCT GGAGCAGCTT GACGCGCTAT GGGAGTGGCT GGCCCACGAT 
CCGGAGTGTG CCGACTGCTA CTTTTCGTGG CTGCAGGCCC AGGCCAAAGG CGGTGATCAG 
CATGCTCTGG GCATTGAAGC ACTTCAGCAT TTGTATTTGA AGAAACTCCC AGAGCTGCGA 
CCCGAGGAGT TCTCTATGGT TGCGTTGGGA CTATTTCAAC AGCTCTGTAG CTTCGCCCGC 
ATCGCCATGG CCGAGTACGA CAATCATAGC GACCAGATCT CGGCGAGCGC CAGTGCCGTT 
GGCATGTACC ATCTTTGGAA GATAGCGTTG CGTGCCCAGA GCAACGATGT CTCCTTGGCG 
GCCATACAGT ACATCAACAT GTACTACATG GGTCAACAAT TGCGCCTGGA GAAGGAGTTC 
GTTTCACAGT GTATGGAAAA CCTAGTTCAA GCGGCCACGG CTTTGGAAAG CATCGATGAT 
GAGAATGCTT TGATGCGTGT CCAGCGTGGC CTGCTCTTGC TGAACACCCA TTTGGATACG 
TTCCGGCGTC GATATGCGTT TCATCTGCGC CGCTGGGCCA TCGAAGGCAA AGGGATTGGA 
TCGCACAGTA ACCTTAAAAA TGAGGGAGCG GGTCCACCAC TACGAATAGT GCTGCAACAG 
GCAGGACTTT CGGAAAAAAG CCTTTTGCAG ATGCACGCGT GTGACTTAAT CGCCGACCTC 
AAGGCTGAGG TGTCCAAGTG GTGGGAGAGC CTTCAAACTG GACTAGCTGC TCCTGTGCTC 
GGTCTGCTGC TCAGCGATGG ACCGCTACGC ATTATCACAC AGGGACAGGA ATTGACCTCG 
GATTACGATG AACGGAGTCT CGGAGATGCT GGTTTCAAGG ATAACCAGAT CGTCTACGTA 
TCGTTAGGTG GACGTGGAGC CAGGCGAAAG GAATCCAATT TGGAGCATCC GTCAATGCTG 
CCGCCCCCAC CAAAGGAGTG TTTGCCCACG GTGCTCCTGC TGCAGCCAAA GTACTTCGAG 
AAGCTATTTT GCCTGATGCA GACCCTGGGC GATATGCAAC CGCAAGCGTC GACTGTAAAT 
CCCCAGCACC ACACAAAGGC GCAATTGCTC TCGCGACGCG TATGGGATAT CCTCGCCATG 
CTGCCAACTA ATCCGCACAT TCTAGACGCC TTTAAGAGTC TGGTTACGGA CCTAAGCGAA 
CTCGAGCAGC TTGATGCTGG CGGAGAGGAG GAGCAACTGG CCACCAAGCG CAAGCAAATC 
AAGCAGAAGT TCCGAGATCT TCTCGACCCG AACAACTTGC AAAAGTTCAT GTACTCCCTC 
CATATTGTGG AGAGTCTGGC CCTAACAAGT TCCAGACGTG GTGAATCCAA CGGCAATGTG 
GCCATGGGAA ATACACCGGA ACAGGTACGG GTGAAGAAAT CCTCGATGGG AAGACGGAGA 
AATAGCAACG AGCAGCCGCC ACCGCCGCCG CCAGAAGTCA AGATGAGCAA GGAGCAACTG 
TGCGAGCTGG AGGCCCCACT GACGCCCACA CCCTCGACTG GCTTGCAGGA TGTGGAAACG 
GAGGCCAGCA GTAGCAGCGG TGGCGACAAA GAAAACCAAC CGAAACAACA CAGCAAGCGT 
CAGAAGAAAG GCGAAACTTT TGAACAGGAA AAGGAGCGCC CAGTGGGTTG CTCCACGCCG 
CCGTCTCCAA CTCCTCCGCC ACCAGCACTT TCGGTAGTGG AAAGAGGTGA CAATAAGTGG 
AGTGAGGCGT TCGTCAAATG TGGTGGCCTT CGTCATCTGT ACGAAATCTT TAGTGAGGGC 
CAACTACAAC AAAGCGCACA TCCCAAGGAG TTGGCTTTAA ATGAATGGCG ACACGATTGC 
CTGGCCAGCC TGCTGCGCAT TCTCTGGCTT CTTGGATTCG AGGAACTGCA GTCGGCGGAT 
GCCCATGTTC TAATGTCACG TCCGCACCCG TTTATGCTAC AGTTGATGGA GGTACCACAG 
TGCCTGACCC GACTCTCATC GATCCTTAAC GACGAAGTTC ACCAGCAGCA TCAGGCTTCT 
TCGGCCAATC CACTGGTATT CCCCTATCAG TTCCAGCACT TGCGTACTGG CTTTTGGGGC 
AGGGCCCAGC TCATCCAGTT CGCTATGAAC ATTCTAGTGA GTTTTGTGCA CGCCTCAGCG 
GAAGCGAGAA GGCTTCTCTG GGCCCCAACT GGTACCGATC ACTGTCGATG GCTGCAGAAG 
TTCATCCTGG AGGATCCCGA ACCGGCGGTA CGGCGTGAGA TATGCGCTGG TCTTTATCGC 
ATTTGCTTAG GAAACGCACA TAGCTATCGC CTCTTACTAG CACCACTGCT GCACAAGCTC 
ATCGCCCTGT TGCCATTGGC CGAGCAGATG AGCTCTGGCA ACCAGCACAC GCAGTTTTTG 
CTCTCGGAAG AGGGCAAGGA TCCCTACGGA CCCGCCTGCC GTGACTACTT TTGGCTGCTG 
GCCAGATTGG TGGATACTCT CTCCCCGGAA ATGGTGGCCG AGGAGCACAT CGACATTGAA 
ATGCTGTGCG AAAGCATCTC GCAGAGCATT CTCACCCGAG AGTATTATGA ACTGCGCCAT 
GGTTACCAGG ACGATGGCTT GGTGGGTCTC CTAAATCTCA TGTCCAATTT GATCAAGTAC 
GACACCACAT TCAAGTACAC ACCCAAGGCG TTATCCTTTA TTGAGCAACT GATTGGCTTC 
CTGTTTGACA TGCCCTCGCC GGCAGATCGC CAGAAGCCCA AGTGCAAGTC GGCGTCGTCT 
CGAGCATCTG CCTACGATCT GCTGGTTGAA CTTTGCCGTG GATGCGCCAC AAACTATGCA 
TATCTGCATG GACGTCTGTT GGCACAGCAC AAATCCGGAC CCAAGCAGCC GTATCCCTGG 
GACTATTGGC CGCGCGATGA AGGCAGAGCT GAGTGCGGAT ATGTGGGGCT AACTAATTTG 
GGAGCCACTT GCTATATGGC CTCTTGCGTC CAGCACTTGT ACATGATGCC ACAGGCGCGA 
GCAGCTGTTT TGCGAGTTCC ACCCAACGCC GCCCGTAAGC ATGGGCCCAC ACTTCTGGAA 
CTACAACGGA TGTTTGCCTA CCTACTGGAG TCGGAACGGA AGTCTTACAA CCCGCGAAGC 
TTCTGCCGGG TGTACCAGAT GGATCACCAG CCGCTAAATA CGGGAGAGCA AAAGGATATG 
GCAGAGTTCT TTATCGATTT GGTGTCCAAA CTGGAAGATA TGACGCCTGA CTTGAAGCAC 
CTGGTGAAAA GGCTCTTCTG TGGATCCTTG AGCAATAATG TCGTCTCCCT GGATTGCGGT 
CACGTTTCCC GCACGGCCGA GGACTTTTAC ACGGTTCGCT GCCAGGTTGC AGATATGCGC 
AATCTGCAGG AATCCCTGGA CGAAGTCACC GTTAAGGATA CCCTCGAGGG CGACAACATG 
TACACCTGCT CGCAGTGCGG CAAAAAAGTA CGAGCCGAGA AGCGGGCTTG CTTCAAGAAG 
CTTCCCCAGA TCTTGTGCTT CAACACCATG CGCTACACCT TCAATATGGT CACCATGCTC 
AAGGAGAAGG TCAACACGCA CTTTTCCTTT CCGCTACGCC TGAACATGTG TCACTATGTG 
GAGAAGACCT TGATGCCGCA GCAGTACAAA GAGGAGCGAG AAAGGCGCCA AAAGGAGAAG 
GAAGGAGCAG ATGGTAGTGG CGATGGGAAT GACAACGAAA AGGCAGAGGC CACGCTAGAT 
GACGATATTG AAGAATGCTA CGAGTACGAA CTGGTGGGCG TCACTGTCCA CACGGGCACA 
GCGGATGGCG GCCACTACTA CAGCTTTATA AAGGAGCGGA CGAAGACTAG TTACCATACG 
CACGAGCGGT GGTTTCTTTT CAACGATGCC GAAGTGAAGC CTTTCGATCC CTCACAGATA 
GCGGCGGAGT GCTTTGGCGG CGAGATGACG AGCAAAACCT ACGACTCAGT GACGGAGAAA 
TATCTGGACT TTAGTTTCGA GAAGACCAAC TCTGCCTACA TGCTGTTCTA CGAGCGCCGA 
CTGCCAGAGC ATCTGCAGCG CCGGCATTCC GAGCTGCTGG TTACACCCAC GCCCAGTCCC 
ACAGTGGAAG AGAAGTCCGA GGCGGAGGAG CCCACTAAAA TGGAGACAAG CTCTAGCGAA 
ATCAAGGCAG ATGTGGATGT GGAAGTGGAA GTTGAGGAGA AGGACAAGGA GAAGCCTGCG 
CAGACTGACA CAGAATCTAA GGAAACGCCT GCAAAAGAGG AGATCGCGGA TGACAAATCG 
AAGCAGGATG AGCCTGAGGA AAAGAAAATC GAAAAGCAAT CCAGAGAGGG CGAAGAGAAA 
TCGGAAACGG ACGAAAAGCC CACGGAGATG ACGACCGTGA GCACTGAGGA GGAAAAGCAG 
CCAACAGCCA ATTGTGATAA TCACCAGCAA AACAACAATA GCAACAGCAA AGCCAGCAAC 
GATCAGCAAC CATCCACGTC GAAGGCTGCC CAGAAGCTAC AACTATTTCG TCCATTGTTG 
AACAAGGAGC TCGAGGATTG GATTTGGCAG GACAATCGAC AGTTCCTGCA GGATCGCAAC 
ATTTTCGAGC ACACATATTT CAACTTTATG TGGCAGATAT GTGGGCACAT ACCCCAATCA 
TTAATATCAG AGACGGACGT CACCTGCATG GCAGCCAAGT TGTCTGTGTC CTTTTTCATT 
GAGACTTTTA TTCATGCTAA GGAAAAGCCC ACTATGGTGC CCTGGGTGGA GCTGCTGACC 
AAGCAGTTCA ATGCCAGCCA AGAGGCCTGC GAGTGGTTTC TGTCGCACAT GTCGCAGGAG 
CCCTACTGGC CGGTCCAGGT GCTAATCCAG TGTCCCAACC AAATGGTGCG TCAAATGTTT 
CAGCGTTTGG TAATCCATGT CATCCAGCAA CTACGTGCCT CACATGCTCA TCTGTATCTG 
GAAGTGGAGA CAGACGAGGA TGACAAGGAG TTAATAGGCC AAGCCTCTTG CGTCACCCGT 
TTCATTGGTT CGCTCATCTC GCTCCTGGAG CACGGTGCCC GAGCGAACCT GCGGCACTTG 
TCCGAGTACT TTGGACTGTT GTGCGAGTTC TCACGGATGG GAGATGAGGA AGCCATGTAC 
TTGCTGCGCA TCGGTGTACT CAAGAGCCTG GTCGACTTCT ATCTGGGTCA CAAGCAAACA 
GACAGCATAG ACATAAGCTC TGACAACGAA GACAACTCCT CTGAAGAGGC GCTGTCCGTG 
GAGAAGATGC GTCCGGCCTC GCTGGACAAG ATGATTGCTC TGTGCGCCAG CCTAGTGGAA 
AGATCAAGAG GAGCCGACTT CCGGCTGCGA CTCTCGCCCA AAGACTTCTC TGCAATCGCC 
GGCGGCAAGG GTTTTCCATT TTTGTACCAA CAAATAAAGG ACGGCATTAA TCCGCACCAG 
ACAAAGCATT TAATACACGC GCTTTGTCGC TGGGATGAGC GCCTGGCCAC GCAGATCATT 
GGCATGCTCT TCGCGTCGGT AACCAAGCAC ACGGAGCTGT GCGCGCCATT CTTCAAGCTG 
CTAACTCTGC TCACCGAGAC GCAGGGCGGT CCAGTGGGCC TGCCTTGTTT CACCCAGTTG 
ATCCTGCCAC GAATGTGGGA CGCCGCCGAG TACTGTCCAC AGTCGGTGCT CGACTGGCTC 
TCGCTGCAGG CCACCAAGAA CAAGATCGCC CACGCCTGGA TACTTCAATC GGCCGAGAAG 
TGGCTCGAGC AATTCCTGCT GGCCCACGAT AACACTCGTG TTCGGAACGC TGCCGCCTTT 
CTGCTGGTGG CACTGGTACC CTCCCAGCCA TTCCGGGCCA ACTTCCGCGC CCACTCGCAA 
CACAAGCTGC TGGCCCTCAA TCCGCACAGT TATCGCGACA TAAATAGCGA TGCCCAGGCG 
GTGCTGCACC AGGTGATCAC GCTGCTCCTG CGATTGCTGC GTCCAGCGCG CGTCTATGCG 
GATATCGGAG CCCATGGCAC GACCAAGCTG ACCGCGTACT TTAATCTGCT CAGCTATTGT 
ATGGTTTCAA AGACTGAAAA ACTAATGATA GGTTCGTACA TGCGCTCCCT GTGGGAGCTG 
TTCCATCCAC GCCTCTCGGA GCCCAGTGTT CCGGCCCATC ACAATAAGCA CGCACTATTA 
ACCTTCTGGC ACCACTCACT GGTGGACTGT CCGGAGAATG CTGCCCAGGT GGCCAACTGC 
CCGGAGATTA CGCGCAACAT TGCATTTAAC TATATACTGG CCGATCATGA TGACGCGGAG 
ATAGTCACCT ACAACCGTTC CATGCTGCCC GCCTATTATG GACTACTTCG TCTGTGCTGC 
GAGCAGAGCC GTGCACTCAC ACGCCAATTG TCCCAGCATC AGAATCTTCA GTGGGCATTT 
AAAAACATCA CACCGCATCC CACGCAGTAC GCGGCCGCCG TGGACGAGCT TTTCAAGTTG 
ATGGCTCTGT TCGCCACCCG TCATCCGGAT GCTAGTGAAC AGGAAAAGCT TGATGTGACC 
CAGTTTAGGA GGGCTGTGAT TGTGTCCTAC ACCAGTAGTC TGGATGCACG TGTCTCTTGG 
TCCACGCTGA TCAGTGCTCT TAAGATACTC GTGGACAATG AGGAGGACCG CACGATGGTG 
ATCTTCAACG GCGGCATTGA GATGTGTTTC GAGGCGCTGC ACACGCTACA TTCCATGCAC 
CACGAGGCCA CCGCCTGCCA CGTGGCCGGA GACCTATTCG ATCTCTTGGG CGAGATGCTA 
CTCCTCCTAG CCACTCTTCG AACCCGCACA GACAGTCCTG CTCAAAAGAA GCAGCAGCAA 
CAACAGCAGC AGCTGGAGCA ACAGTTGCAG TTGCAGCAGC AACAGATGCT GCAGAAACAA 
CAGCAGCAGT CGCAAAGCCA AACGCAAACA CCGCAGTCGC CGCAGCAAAA GGAAAAGCAG 
CTGCAGCAAC AAATGCAGCA GCATCTGCAG CTGCAACAGT TGCAGCAAAT GCAGTTCCAG 
CAGCAACACT TCCTGCGCCA GCAACATCAA CACTCGGCGC TGGCAAAGGC ACTGCCGGAT 
GCAGTGAAGC GACTGGCTAC GCTACTCAAC ACCTTCAATT CGCCAGAGAT CAGTCGCATG 
GCCTTGGAGG TGCTCAAGGA GCTGGTGCGC AATCCCAGCC TGGAAACCAT TAGTATTCTG 
GCGCCCATTC TTATCAACTG TCATCTGTCC GTGGCGAATG CTCCCAATGC GATCGGTCCT 
CTGGGACCGT ACTTTCCGCG TCGCGGTGCG AAGCATACGC CTTGGCCATT GGGAGCCAAG 
AACTCACCCC GTCCACCGCG GCCCATGGTA CAGATGTGCA TCGCTCTGGC TGAGCTGACA 
CCACGCGGTC TCGATGCCGA TTACGACGTT CAGGTGGAGT CCTTTTACCG ACCCTACCAT 
GACTTTATCG ATGTCATGAT GCGCATGTGC GTCAACACGG GCACTCTGAA CGACACGCTA 
GTCAAGCTGC AATGCTTGGT GGCCATCGAG TCCACGCCGC TGCACTTCAC CTATTTCCCC 
AAATTCTGGG TGGGCATACA CAACAATGCT CTGACGCACA AATACGTTGA GCTGCTGGTG 
AAAAATCAAC TGTTGGTAGA GTACCTTCAC AATGTCCTTA GGGACGAGCG AAGCATGTTG 
AAGGATGCCT GCGTTCGTGA ATTTCTGGAG CTCTATTATC ACAAGGTTGC CGCCCAGCTG 
CCAGTGGCCC GTATGATCTA CACAATCAAC TATGGTATGC ACTCCAAGGA CGACATTGAT 
GAGCTGTGTG GCGATCTGTT CGCCATCCGA ATCATTGCCC AGGCCACTGG AGTGCCGGCC 
TCAGTGCGCA AGGAGTTGCG AGGATCGCTG AGGGCGCTTC AGAACAAGAG CGAGCGGTTT 
AGGAAGGAAT CGGAGAGGGA TCCATTTCCG AACAAGAAGC AGAAACGTGA CTCGCAAAAG 
ATCAAGGAAA AGGAGCACCC TCAGCCGGAG AGCGAGAAAG AGACCTCAAC GGAGAACGAC 
AAACCATCAG ATGTTTCCAT GGAATCCAGC GGTAACGCGG AGCAAGCAAC GGACAGCACA 
AAACCACCAA CTCCTGCAGG CAGCGACGAT GAGCAGATGG ACAAAACTTC AGTTCCCTCG 
TCCGATGACG AGACAGAGTT GGAGGACGAG CTGCAGCCGA CGCCCAAGCG CAACAAGAAG 
GCTTCGAATA AGAAGACCGC CCAGGATCGT GTCAACGAGG AGCGCGAGAA GCGACTTATC 
ACGCTGATCA CCATGGAATC ATATATCCAG AGCATTTTTG CCATCCTTAA GAGAGATGCC 
AGTGTCCCCA GTTCGGGAGC GACTAAGGAG CCCAGTGAGG AACCGTGTGG CGAGGCATCC 
ACTTCGGCGG CTGCCGCTGT AAAGTTATCT CGTCCCAAAG GAGCCTGTAC TCCGCCGGAG 
CCAATTACCG ACGTAAACGA TACGCGTTGC AACATAGATA CTGAGACAGA GGCGGAGGCG 
GATGAGCAAT CTCCAAAGAC GAGCCAGACA AATGGTAGCC AACAGAACGA ATCCCCGCCA 
GCGGCGACCA GTGCAGACAC TGCACCCGCG AATCCTTCAC CAGCTCCCGC GGCCGCCGTT 
GCGTCCACAT CCCAGGCGGC AAGTCCAACA CAGATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733022.2
CDS	358..12096
Translation	MCEVCADFQNLLELYEVRVASSDLKFQLLLKSEIETTFNYIQSWPQRQCMCLYRDTKNYDRFNLVVQSLICLTVQHLKHIDHLIDNYKRLTASAAQVVAQAQQQREQQRDEASAQAEAKESSAPAEEPKKEEPSGSAGEEAQGSGDGPAKKPPVGPCTPPPPQTANPQHKSHLQYTEEPWILPEVEKLLVLVSKVFLLNFPLYIAHKHGMHSRLDDLQAEEAHHLALICDLHDNDLPIYLLRNVSLFCNSGGFGAMSLCFEHPDLPVSTAHSMTAAVSNVKLWLNYHCNTQLFVPLRSRILQYMCKLSDQSLRSAATRAMADFVWSSMRDPLDVAVNFDTEGLALAFKYFTSTTLTMRLAGMAQINAHINLFNEICTTETVNEVELFGQRIANWLTENHIVQHLFGPNLHVEIVKQAHVLLNFLAVENQISEEDIKLIWQATQLKHCSKTIFDILPSLVKNLTPRPAMHLYSLLCRMDPKEHTEQSIYIASALTKQLWTRDTSRSQMNLMQDHLLGSNVTASSSDSGSIEGSNTEDDHVGADDSSIASGGGGGGVGNKSPIDGVTPCKQARHRRHICDPTTEKGKQISPEDMAKVDLVNKRIVNIIDNTSSEEELQSRAALELQLHRSRKKTSNKRRRQKTNKQIILPHELVEIWDGVEDVPSSDEADGEADGDGEGELLADSDECSDGATSQLVPDAVLKHLQGEGPFIRAIETNINELLSGAENDGSYSSPMSNKSEKNLADFDDEDVSPCEEELAQLVSSRANCSDVPPAFAAAAAAMMVAQSAMALQKSGESNVAAAAAAVAAAAAATGTAQQTLVAMNRQASVAAAAAVVAAAKAKSDSDVDLMDVVSGGKQHHSPKASQGSSTSGSTPVQPSFKLNDVCQPGNTLLWDLLQDDKIGQLGESLALEAEKALATLLCFSMDRQLRTKFIEGCLYNVANNRSVIVSLRLLPKLFASFQQFRPSDTHSMTMWAERNHRMMQCFFNNIRHYARRHAEVLITQNGEQQQQQLGGQLYSHKTQVSVRLQFLSSIFSTVGSPKSFRLTLEQLDALWEWLAHDPECADCYFSWLQAQAKGGDQHALGIEALQHLYLKKLPELRPEEFSMVALGLFQQLCSFARIAMAEYDNHSDQISASASAVGMYHLWKIALRAQSNDVSLAAIQYINMYYMGQQLRLEKEFVSQCMENLVQAATALESIDDENALMRVQRGLLLLNTHLDTFRRRYAFHLRRWAIEGKGIGSHSNLKNEGAGPPLRIVLQQAGLSEKSLLQMHACDLIADLKAEVSKWWESLQTGLAAPVLGLLLSDGPLRIITQGQELTSDYDERSLGDAGFKDNQIVYVSLGGRGARRKESNLEHPSMLPPPPKECLPTVLLLQPKYFEKLFCLMQTLGDMQPQASTVNPQHHTKAQLLSRRVWDILAMLPTNPHILDAFKSLVTDLSELEQLDAGGEEEQLATKRKQIKQKFRDLLDPNNLQKFMYSLHIVESLALTSSRRGESNGNVAMGNTPEQVRVKKSSMGRRRNSNEQPPPPPPEVKMSKEQLCELEAPLTPTPSTGLQDVETEASSSSGGDKENQPKQHSKRQKKGETFEQEKERPVGCSTPPSPTPPPPALSVVERGDNKWSEAFVKCGGLRHLYEIFSEGQLQQSAHPKELALNEWRHDCLASLLRILWLLGFEELQSADAHVLMSRPHPFMLQLMEVPQCLTRLSSILNDEVHQQHQASSANPLVFPYQFQHLRTGFWGRAQLIQFAMNILVSFVHASAEARRLLWAPTGTDHCRWLQKFILEDPEPAVRREICAGLYRICLGNAHSYRLLLAPLLHKLIALLPLAEQMSSGNQHTQFLLSEEGKDPYGPACRDYFWLLARLVDTLSPEMVAEEHIDIEMLCESISQSILTREYYELRHGYQDDGLVGLLNLMSNLIKYDTTFKYTPKALSFIEQLIGFLFDMPSPADRQKPKCKSASSRASAYDLLVELCRGCATNYAYLHGRLLAQHKSGPKQPYPWDYWPRDEGRAECGYVGLTNLGATCYMASCVQHLYMMPQARAAVLRVPPNAARKHGPTLLELQRMFAYLLESERKSYNPRSFCRVYQMDHQPLNTGEQKDMAEFFIDLVSKLEDMTPDLKHLVKRLFCGSLSNNVVSLDCGHVSRTAEDFYTVRCQVADMRNLQESLDEVTVKDTLEGDNMYTCSQCGKKVRAEKRACFKKLPQILCFNTMRYTFNMVTMLKEKVNTHFSFPLRLNMCHYVEKTLMPQQYKEERERRQKEKEGADGSGDGNDNEKAEATLDDDIEECYEYELVGVTVHTGTADGGHYYSFIKERTKTSYHTHERWFLFNDAEVKPFDPSQIAAECFGGEMTSKTYDSVTEKYLDFSFEKTNSAYMLFYERRLPEHLQRRHSELLVTPTPSPTVEEKSEAEEPTKMETSSSEIKADVDVEVEVEEKDKEKPAQTDTESKETPAKEEIADDKSKQDEPEEKKIEKQSREGEEKSETDEKPTEMTTVSTEEEKQPTANCDNHQQNNNSNSKASNDQQPSTSKAAQKLQLFRPLLNKELEDWIWQDNRQFLQDRNIFEHTYFNFMWQICGHIPQSLISETDVTCMAAKLSVSFFIETFIHAKEKPTMVPWVELLTKQFNASQEACEWFLSHMSQEPYWPVQVLIQCPNQMVRQMFQRLVIHVIQQLRASHAHLYLEVETDEDDKELIGQASCVTRFIGSLISLLEHGARANLRHLSEYFGLLCEFSRMGDEEAMYLLRIGVLKSLVDFYLGHKQTDSIDISSDNEDNSSEEALSVEKMRPASLDKMIALCASLVERSRGADFRLRLSPKDFSAIAGGKGFPFLYQQIKDGINPHQTKHLIHALCRWDERLATQIIGMLFASVTKHTELCAPFFKLLTLLTETQGGPVGLPCFTQLILPRMWDAAEYCPQSVLDWLSLQATKNKIAHAWILQSAEKWLEQFLLAHDNTRVRNAAAFLLVALVPSQPFRANFRAHSQHKLLALNPHSYRDINSDAQAVLHQVITLLLRLLRPARVYADIGAHGTTKLTAYFNLLSYCMVSKTEKLMIGSYMRSLWELFHPRLSEPSVPAHHNKHALLTFWHHSLVDCPENAAQVANCPEITRNIAFNYILADHDDAEIVTYNRSMLPAYYGLLRLCCEQSRALTRQLSQHQNLQWAFKNITPHPTQYAAAVDELFKLMALFATRHPDASEQEKLDVTQFRRAVIVSYTSSLDARVSWSTLISALKILVDNEEDRTMVIFNGGIEMCFEALHTLHSMHHEATACHVAGDLFDLLGEMLLLLATLRTRTDSPAQKKQQQQQQQLEQQLQLQQQQMLQKQQQQSQSQTQTPQSPQQKEKQLQQQMQQHLQLQQLQQMQFQQQHFLRQQHQHSALAKALPDAVKRLATLLNTFNSPEISRMALEVLKELVRNPSLETISILAPILINCHLSVANAPNAIGPLGPYFPRRGAKHTPWPLGAKNSPRPPRPMVQMCIALAELTPRGLDADYDVQVESFYRPYHDFIDVMMRMCVNTGTLNDTLVKLQCLVAIESTPLHFTYFPKFWVGIHNNALTHKYVELLVKNQLLVEYLHNVLRDERSMLKDACVREFLELYYHKVAAQLPVARMIYTINYGMHSKDDIDELCGDLFAIRIIAQATGVPASVRKELRGSLRALQNKSERFRKESERDPFPNKKQKRDSQKIKEKEHPQPESEKETSTENDKPSDVSMESSGNAEQATDSTKPPTPAGSDDEQMDKTSVPSSDDETELEDELQPTPKRNKKASNKKTAQDRVNEEREKRLITLITMESYIQSIFAILKRDASVPSSGATKEPSEEPCGEASTSAAAAVKLSRPKGACTPPEPITDVNDTRCNIDTETEAEADEQSPKTSQTNGSQQNESPPAATSADTAPANPSPAPAAAVASTSQAASPTQI

Target ORF information:

RefSeq Version	NM_170158.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster puffyeye (puf), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170158.3

ORF Insert Sequence:

ATGTGCGAGG TGTGCGCCGA CTTCCAGAAT CTCCTGGAGC TCTATGAGGT GCGCGTGGCC 
AGCTCGGATC TGAAGTTCCA GCTGCTGCTG AAGAGCGAGA TCGAGACCAC CTTCAACTAC 
ATCCAGTCGT GGCCGCAGCG TCAGTGCATG TGCCTGTACC GGGACACCAA GAACTATGAC 
CGTTTCAACC TGGTAGTGCA GTCGCTCATC TGTCTGACGG TGCAGCATCT GAAGCACATC 
GACCACCTCA TCGACAACTA CAAGCGGCTA ACGGCCAGTG CTGCCCAGGT GGTGGCCCAG 
GCCCAGCAGC AGCGTGAACA GCAGCGGGAC GAAGCGTCAG CCCAGGCAGA GGCAAAGGAA 
TCATCCGCGC CAGCGGAAGA ACCTAAGAAG GAGGAGCCAT CTGGGTCCGC CGGTGAGGAG 
GCTCAGGGCT CCGGCGACGG GCCAGCCAAA AAGCCGCCAG TTGGCCCCTG CACTCCCCCT 
CCCCCGCAAA CGGCCAATCC TCAGCACAAA TCCCACCTGC AGTACACAGA GGAGCCCTGG 
ATCCTGCCCG AGGTGGAGAA ACTGTTGGTG CTCGTTTCAA AGGTGTTCCT GCTCAACTTC 
CCGCTGTACA TCGCCCACAA ACATGGCATG CACTCTCGGC TGGACGACCT GCAGGCTGAG 
GAGGCCCACC ACCTGGCCCT CATCTGCGAT CTTCACGACA ACGACCTGCC CATCTATCTG 
CTGCGGAATG TGAGCCTGTT CTGCAACTCG GGCGGCTTTG GCGCCATGTC GCTGTGCTTC 
GAACATCCCG ATCTGCCAGT GTCCACCGCT CACTCGATGA CCGCTGCCGT CTCAAATGTG 
AAACTTTGGC TGAACTATCA CTGTAATACT CAGCTCTTCG TGCCGCTGCG AAGTCGGATA 
CTGCAGTACA TGTGCAAGCT GTCGGATCAG AGCCTCCGAT CTGCCGCAAC CCGCGCGATG 
GCCGATTTTG TGTGGTCCTC TATGCGAGAC CCACTGGATG TTGCTGTCAA CTTCGACACC 
GAAGGATTGG CCCTGGCCTT TAAGTACTTC ACATCGACCA CGCTAACCAT GCGACTGGCA 
GGCATGGCCC AGATCAACGC ACACATTAAT CTATTTAACG AGATCTGCAC CACCGAAACG 
GTCAACGAAG TGGAGCTGTT CGGGCAGCGC ATCGCGAACT GGCTGACTGA AAATCATATT 
GTACAGCACC TGTTTGGCCC GAATCTGCAC GTAGAAATCG TTAAACAGGC ACACGTGCTG 
CTCAACTTTT TGGCGGTAGA GAACCAGATC TCTGAAGAGG ACATCAAGCT GATCTGGCAA 
GCCACCCAGC TGAAGCACTG CTCAAAGACG ATCTTCGACA TCCTGCCTTC GCTGGTGAAG 
AATCTGACCC CGCGCCCGGC AATGCACCTT TACTCCCTCC TTTGCCGCAT GGATCCCAAG 
GAGCACACTG AGCAGAGCAT ATACATAGCC TCAGCGCTAA CAAAACAGCT GTGGACTCGT 
GATACCTCAC GCAGTCAGAT GAACCTGATG CAAGACCACC TGCTGGGGTC CAACGTCACC 
GCTTCCAGCT CTGACAGCGG CAGCATTGAG GGCAGCAACA CAGAGGACGA CCACGTGGGC 
GCCGACGACA GTAGCATTGC CAGCGGTGGC GGCGGCGGAG GTGTTGGTAA CAAGAGTCCA 
ATCGATGGAG TTACTCCATG CAAGCAGGCC CGGCACAGGC GGCACATCTG TGACCCCACC 
ACCGAGAAGG GAAAGCAAAT AAGTCCCGAG GATATGGCCA AAGTAGACTT GGTGAACAAA 
CGGATTGTGA ACATTATCGA CAACACAAGC AGTGAAGAGG AGCTGCAATC GCGGGCCGCA 
CTGGAGCTGC AACTGCATCG CAGCCGCAAA AAGACGAGCA ACAAGCGACG GCGACAAAAA 
ACCAACAAAC AGATCATTCT GCCCCATGAG CTGGTCGAGA TTTGGGACGG AGTTGAAGAT 
GTGCCCAGCA GTGACGAGGC TGATGGCGAA GCCGATGGTG ATGGCGAGGG CGAACTTTTA 
GCGGACAGCG ATGAGTGCAG CGATGGTGCT ACGAGTCAGC TGGTACCCGA CGCCGTGCTA 
AAGCATCTGC AGGGAGAGGG ACCTTTTATC AGGGCCATCG AGACCAATAT CAATGAGCTG 
CTCAGTGGTG CCGAGAACGA TGGCAGTTAC TCATCCCCGA TGAGTAACAA ATCTGAAAAG 
AACCTGGCTG ACTTCGATGA CGAGGACGTG TCGCCTTGCG AGGAGGAGCT GGCCCAGCTG 
GTCAGCTCCC GCGCCAATTG TTCGGACGTT CCACCAGCCT TTGCAGCCGC TGCCGCCGCC 
ATGATGGTGG CCCAGTCGGC GATGGCCCTT CAGAAGTCTG GAGAGTCGAA TGTGGCTGCT 
GCTGCCGCTG CTGTGGCCGC TGCTGCAGCG GCTACCGGAA CTGCACAGCA AACGCTGGTG 
GCAATGAACC GGCAGGCCAG CGTTGCTGCA GCTGCCGCCG TGGTTGCTGC GGCCAAGGCA 
AAATCGGACT CAGATGTCGA TCTGATGGAT GTGGTTTCCG GCGGAAAGCA GCACCATTCA 
CCAAAGGCGA GCCAGGGCAG CTCTACGTCC GGGAGTACAC CGGTGCAGCC CTCGTTCAAG 
CTAAATGATG TCTGCCAGCC CGGAAACACA CTGCTTTGGG ATCTGCTTCA AGACGATAAA 
ATTGGCCAAC TAGGCGAGTC TCTGGCTCTA GAGGCGGAGA AAGCTCTGGC CACTCTCCTG 
TGCTTCAGCA TGGACCGCCA ACTGCGAACG AAATTCATCG AGGGTTGTCT GTATAACGTC 
GCCAACAACC GGAGTGTTAT TGTCAGTCTG CGACTGCTGC CCAAACTGTT CGCCTCGTTC 
CAACAGTTTC GACCCTCGGA CACGCACTCT ATGACGATGT GGGCAGAGCG AAATCACCGG 
ATGATGCAGT GCTTTTTCAA TAATATACGT CACTATGCGC GTCGCCATGC CGAGGTACTG 
ATCACCCAAA ACGGAGAGCA GCAGCAGCAG CAGCTGGGCG GACAACTCTA CTCGCACAAG 
ACACAAGTTT CTGTAAGACT GCAGTTCCTA TCATCGATAT TCTCGACGGT GGGATCCCCG 
AAAAGCTTCC GCCTAACGCT GGAGCAGCTT GACGCGCTAT GGGAGTGGCT GGCCCACGAT 
CCGGAGTGTG CCGACTGCTA CTTTTCGTGG CTGCAGGCCC AGGCCAAAGG CGGTGATCAG 
CATGCTCTGG GCATTGAAGC ACTTCAGCAT TTGTATTTGA AGAAACTCCC AGAGCTGCGA 
CCCGAGGAGT TCTCTATGGT TGCGTTGGGA CTATTTCAAC AGCTCTGTAG CTTCGCCCGC 
ATCGCCATGG CCGAGTACGA CAATCATAGC GACCAGATCT CGGCGAGCGC CAGTGCCGTT 
GGCATGTACC ATCTTTGGAA GATAGCGTTG CGTGCCCAGA GCAACGATGT CTCCTTGGCG 
GCCATACAGT ACATCAACAT GTACTACATG GGTCAACAAT TGCGCCTGGA GAAGGAGTTC 
GTTTCACAGT GTATGGAAAA CCTAGTTCAA GCGGCCACGG CTTTGGAAAG CATCGATGAT 
GAGAATGCTT TGATGCGTGT CCAGCGTGGC CTGCTCTTGC TGAACACCCA TTTGGATACG 
TTCCGGCGTC GATATGCGTT TCATCTGCGC CGCTGGGCCA TCGAAGGCAA AGGGATTGGA 
TCGCACAGTA ACCTTAAAAA TGAGGGAGCG GGTCCACCAC TACGAATAGT GCTGCAACAG 
GCAGGACTTT CGGAAAAAAG CCTTTTGCAG ATGCACGCGT GTGACTTAAT CGCCGACCTC 
AAGGCTGAGG TGTCCAAGTG GTGGGAGAGC CTTCAAACTG GACTAGCTGC TCCTGTGCTC 
GGTCTGCTGC TCAGCGATGG ACCGCTACGC ATTATCACAC AGGGACAGGA ATTGACCTCG 
GATTACGATG AACGGAGTCT CGGAGATGCT GGTTTCAAGG ATAACCAGAT CGTCTACGTA 
TCGTTAGGTG GACGTGGAGC CAGGCGAAAG GAATCCAATT TGGAGCATCC GTCAATGCTG 
CCGCCCCCAC CAAAGGAGTG TTTGCCCACG GTGCTCCTGC TGCAGCCAAA GTACTTCGAG 
AAGCTATTTT GCCTGATGCA GACCCTGGGC GATATGCAAC CGCAAGCGTC GACTGTAAAT 
CCCCAGCACC ACACAAAGGC GCAATTGCTC TCGCGACGCG TATGGGATAT CCTCGCCATG 
CTGCCAACTA ATCCGCACAT TCTAGACGCC TTTAAGAGTC TGGTTACGGA CCTAAGCGAA 
CTCGAGCAGC TTGATGCTGG CGGAGAGGAG GAGCAACTGG CCACCAAGCG CAAGCAAATC 
AAGCAGAAGT TCCGAGATCT TCTCGACCCG AACAACTTGC AAAAGTTCAT GTACTCCCTC 
CATATTGTGG AGAGTCTGGC CCTAACAAGT TCCAGACGTG GTGAATCCAA CGGCAATGTG 
GCCATGGGAA ATACACCGGA ACAGGTACGG GTGAAGAAAT CCTCGATGGG AAGACGGAGA 
AATAGCAACG AGCAGCCGCC ACCGCCGCCG CCAGAAGTCA AGATGAGCAA GGAGCAACTG 
TGCGAGCTGG AGGCCCCACT GACGCCCACA CCCTCGACTG GCTTGCAGGA TGTGGAAACG 
GAGGCCAGCA GTAGCAGCGG TGGCGACAAA GAAAACCAAC CGAAACAACA CAGCAAGCGT 
CAGAAGAAAG GCGAAACTTT TGAACAGGAA AAGGAGCGCC CAGTGGGTTG CTCCACGCCG 
CCGTCTCCAA CTCCTCCGCC ACCAGCACTT TCGGTAGTGG AAAGAGGTGA CAATAAGTGG 
AGTGAGGCGT TCGTCAAATG TGGTGGCCTT CGTCATCTGT ACGAAATCTT TAGTGAGGGC 
CAACTACAAC AAAGCGCACA TCCCAAGGAG TTGGCTTTAA ATGAATGGCG ACACGATTGC 
CTGGCCAGCC TGCTGCGCAT TCTCTGGCTT CTTGGATTCG AGGAACTGCA GTCGGCGGAT 
GCCCATGTTC TAATGTCACG TCCGCACCCG TTTATGCTAC AGTTGATGGA GGTACCACAG 
TGCCTGACCC GACTCTCATC GATCCTTAAC GACGAAGTTC ACCAGCAGCA TCAGGCTTCT 
TCGGCCAATC CACTGGTATT CCCCTATCAG TTCCAGCACT TGCGTACTGG CTTTTGGGGC 
AGGGCCCAGC TCATCCAGTT CGCTATGAAC ATTCTAGTGA GTTTTGTGCA CGCCTCAGCG 
GAAGCGAGAA GGCTTCTCTG GGCCCCAACT GGTACCGATC ACTGTCGATG GCTGCAGAAG 
TTCATCCTGG AGGATCCCGA ACCGGCGGTA CGGCGTGAGA TATGCGCTGG TCTTTATCGC 
ATTTGCTTAG GAAACGCACA TAGCTATCGC CTCTTACTAG CACCACTGCT GCACAAGCTC 
ATCGCCCTGT TGCCATTGGC CGAGCAGATG AGCTCTGGCA ACCAGCACAC GCAGTTTTTG 
CTCTCGGAAG AGGGCAAGGA TCCCTACGGA CCCGCCTGCC GTGACTACTT TTGGCTGCTG 
GCCAGATTGG TGGATACTCT CTCCCCGGAA ATGGTGGCCG AGGAGCACAT CGACATTGAA 
ATGCTGTGCG AAAGCATCTC GCAGAGCATT CTCACCCGAG AGTATTATGA ACTGCGCCAT 
GGTTACCAGG ACGATGGCTT GGTGGGTCTC CTAAATCTCA TGTCCAATTT GATCAAGTAC 
GACACCACAT TCAAGTACAC ACCCAAGGCG TTATCCTTTA TTGAGCAACT GATTGGCTTC 
CTGTTTGACA TGCCCTCGCC GGCAGATCGC CAGAAGCCCA AGTGCAAGTC GGCGTCGTCT 
CGAGCATCTG CCTACGATCT GCTGGTTGAA CTTTGCCGTG GATGCGCCAC AAACTATGCA 
TATCTGCATG GACGTCTGTT GGCACAGCAC AAATCCGGAC CCAAGCAGCC GTATCCCTGG 
GACTATTGGC CGCGCGATGA AGGCAGAGCT GAGTGCGGAT ATGTGGGGCT AACTAATTTG 
GGAGCCACTT GCTATATGGC CTCTTGCGTC CAGCACTTGT ACATGATGCC ACAGGCGCGA 
GCAGCTGTTT TGCGAGTTCC ACCCAACGCC GCCCGTAAGC ATGGGCCCAC ACTTCTGGAA 
CTACAACGGA TGTTTGCCTA CCTACTGGAG TCGGAACGGA AGTCTTACAA CCCGCGAAGC 
TTCTGCCGGG TGTACCAGAT GGATCACCAG CCGCTAAATA CGGGAGAGCA AAAGGATATG 
GCAGAGTTCT TTATCGATTT GGTGTCCAAA CTGGAAGATA TGACGCCTGA CTTGAAGCAC 
CTGGTGAAAA GGCTCTTCTG TGGATCCTTG AGCAATAATG TCGTCTCCCT GGATTGCGGT 
CACGTTTCCC GCACGGCCGA GGACTTTTAC ACGGTTCGCT GCCAGGTTGC AGATATGCGC 
AATCTGCAGG AATCCCTGGA CGAAGTCACC GTTAAGGATA CCCTCGAGGG CGACAACATG 
TACACCTGCT CGCAGTGCGG CAAAAAAGTA CGAGCCGAGA AGCGGGCTTG CTTCAAGAAG 
CTTCCCCAGA TCTTGTGCTT CAACACCATG CGCTACACCT TCAATATGGT CACCATGCTC 
AAGGAGAAGG TCAACACGCA CTTTTCCTTT CCGCTACGCC TGAACATGTG TCACTATGTG 
GAGAAGACCT TGATGCCGCA GCAGTACAAA GAGGAGCGAG AAAGGCGCCA AAAGGAGAAG 
GAAGGAGCAG ATGGTAGTGG CGATGGGAAT GACAACGAAA AGGCAGAGGC CACGCTAGAT 
GACGATATTG AAGAATGCTA CGAGTACGAA CTGGTGGGCG TCACTGTCCA CACGGGCACA 
GCGGATGGCG GCCACTACTA CAGCTTTATA AAGGAGCGGA CGAAGACTAG TTACCATACG 
CACGAGCGGT GGTTTCTTTT CAACGATGCC GAAGTGAAGC CTTTCGATCC CTCACAGATA 
GCGGCGGAGT GCTTTGGCGG CGAGATGACG AGCAAAACCT ACGACTCAGT GACGGAGAAA 
TATCTGGACT TTAGTTTCGA GAAGACCAAC TCTGCCTACA TGCTGTTCTA CGAGCGCCGA 
CTGCCAGAGC ATCTGCAGCG CCGGCATTCC GAGCTGCTGG TTACACCCAC GCCCAGTCCC 
ACAGTGGAAG AGAAGTCCGA GGCGGAGGAG CCCACTAAAA TGGAGACAAG CTCTAGCGAA 
ATCAAGGCAG ATGTGGATGT GGAAGTGGAA GTTGAGGAGA AGGACAAGGA GAAGCCTGCG 
CAGACTGACA CAGAATCTAA GGAAACGCCT GCAAAAGAGG AGATCGCGGA TGACAAATCG 
AAGCAGGATG AGCCTGAGGA AAAGAAAATC GAAAAGCAAT CCAGAGAGGG CGAAGAGAAA 
TCGGAAACGG ACGAAAAGCC CACGGAGATG ACGACCGTGA GCACTGAGGA GGAAAAGCAG 
CCAACAGCCA ATTGTGATAA TCACCAGCAA AACAACAATA GCAACAGCAA AGCCAGCAAC 
GATCAGCAAC CATCCACGTC GAAGGCTGCC CAGAAGCTAC AACTATTTCG TCCATTGTTG 
AACAAGGAGC TCGAGGATTG GATTTGGCAG GACAATCGAC AGTTCCTGCA GGATCGCAAC 
ATTTTCGAGC ACACATATTT CAACTTTATG TGGCAGATAT GTGGGCACAT ACCCCAATCA 
TTAATATCAG AGACGGACGT CACCTGCATG GCAGCCAAGT TGTCTGTGTC CTTTTTCATT 
GAGACTTTTA TTCATGCTAA GGAAAAGCCC ACTATGGTGC CCTGGGTGGA GCTGCTGACC 
AAGCAGTTCA ATGCCAGCCA AGAGGCCTGC GAGTGGTTTC TGTCGCACAT GTCGCAGGAG 
CCCTACTGGC CGGTCCAGGT GCTAATCCAG TGTCCCAACC AAATGGTGCG TCAAATGTTT 
CAGCGTTTGG TAATCCATGT CATCCAGCAA CTACGTGCCT CACATGCTCA TCTGTATCTG 
GAAGTGGAGA CAGACGAGGA TGACAAGGAG TTAATAGGCC AAGCCTCTTG CGTCACCCGT 
TTCATTGGTT CGCTCATCTC GCTCCTGGAG CACGGTGCCC GAGCGAACCT GCGGCACTTG 
TCCGAGTACT TTGGACTGTT GTGCGAGTTC TCACGGATGG GAGATGAGGA AGCCATGTAC 
TTGCTGCGCA TCGGTGTACT CAAGAGCCTG GTCGACTTCT ATCTGGGTCA CAAGCAAACA 
GACAGCATAG ACATAAGCTC TGACAACGAA GACAACTCCT CTGAAGAGGC GCTGTCCGTG 
GAGAAGATGC GTCCGGCCTC GCTGGACAAG ATGATTGCTC TGTGCGCCAG CCTAGTGGAA 
AGATCAAGAG GAGCCGACTT CCGGCTGCGA CTCTCGCCCA AAGACTTCTC TGCAATCGCC 
GGCGGCAAGG GTTTTCCATT TTTGTACCAA CAAATAAAGG ACGGCATTAA TCCGCACCAG 
ACAAAGCATT TAATACACGC GCTTTGTCGC TGGGATGAGC GCCTGGCCAC GCAGATCATT 
GGCATGCTCT TCGCGTCGGT AACCAAGCAC ACGGAGCTGT GCGCGCCATT CTTCAAGCTG 
CTAACTCTGC TCACCGAGAC GCAGGGCGGT CCAGTGGGCC TGCCTTGTTT CACCCAGTTG 
ATCCTGCCAC GAATGTGGGA CGCCGCCGAG TACTGTCCAC AGTCGGTGCT CGACTGGCTC 
TCGCTGCAGG CCACCAAGAA CAAGATCGCC CACGCCTGGA TACTTCAATC GGCCGAGAAG 
TGGCTCGAGC AATTCCTGCT GGCCCACGAT AACACTCGTG TTCGGAACGC TGCCGCCTTT 
CTGCTGGTGG CACTGGTACC CTCCCAGCCA TTCCGGGCCA ACTTCCGCGC CCACTCGCAA 
CACAAGCTGC TGGCCCTCAA TCCGCACAGT TATCGCGACA TAAATAGCGA TGCCCAGGCG 
GTGCTGCACC AGGTGATCAC GCTGCTCCTG CGATTGCTGC GTCCAGCGCG CGTCTATGCG 
GATATCGGAG CCCATGGCAC GACCAAGCTG ACCGCGTACT TTAATCTGCT CAGCTATTGT 
ATGGTTTCAA AGACTGAAAA ACTAATGATA GGTTCGTACA TGCGCTCCCT GTGGGAGCTG 
TTCCATCCAC GCCTCTCGGA GCCCAGTGTT CCGGCCCATC ACAATAAGCA CGCACTATTA 
ACCTTCTGGC ACCACTCACT GGTGGACTGT CCGGAGAATG CTGCCCAGGT GGCCAACTGC 
CCGGAGATTA CGCGCAACAT TGCATTTAAC TATATACTGG CCGATCATGA TGACGCGGAG 
ATAGTCACCT ACAACCGTTC CATGCTGCCC GCCTATTATG GACTACTTCG TCTGTGCTGC 
GAGCAGAGCC GTGCACTCAC ACGCCAATTG TCCCAGCATC AGAATCTTCA GTGGGCATTT 
AAAAACATCA CACCGCATCC CACGCAGTAC GCGGCCGCCG TGGACGAGCT TTTCAAGTTG 
ATGGCTCTGT TCGCCACCCG TCATCCGGAT GCTAGTGAAC AGGAAAAGCT TGATGTGACC 
CAGTTTAGGA GGGCTGTGAT TGTGTCCTAC ACCAGTAGTC TGGATGCACG TGTCTCTTGG 
TCCACGCTGA TCAGTGCTCT TAAGATACTC GTGGACAATG AGGAGGACCG CACGATGGTG 
ATCTTCAACG GCGGCATTGA GATGTGTTTC GAGGCGCTGC ACACGCTACA TTCCATGCAC 
CACGAGGCCA CCGCCTGCCA CGTGGCCGGA GACCTATTCG ATCTCTTGGG CGAGATGCTA 
CTCCTCCTAG CCACTCTTCG AACCCGCACA GACAGTCCTG CTCAAAAGAA GCAGCAGCAA 
CAACAGCAGC AGCTGGAGCA ACAGTTGCAG TTGCAGCAGC AACAGATGCT GCAGAAACAA 
CAGCAGCAGT CGCAAAGCCA AACGCAAACA CCGCAGTCGC CGCAGCAAAA GGAAAAGCAG 
CTGCAGCAAC AAATGCAGCA GCATCTGCAG CTGCAACAGT TGCAGCAAAT GCAGTTCCAG 
CAGCAACACT TCCTGCGCCA GCAACATCAA CACTCGGCGC TGGCAAAGGC ACTGCCGGAT 
GCAGTGAAGC GACTGGCTAC GCTACTCAAC ACCTTCAATT CGCCAGAGAT CAGTCGCATG 
GCCTTGGAGG TGCTCAAGGA GCTGGTGCGC AATCCCAGCC TGGAAACCAT TAGTATTCTG 
GCGCCCATTC TTATCAACTG TCATCTGTCC GTGGCGAATG CTCCCAATGC GATCGGTCCT 
CTGGGACCGT ACTTTCCGCG TCGCGGTGCG AAGCATACGC CTTGGCCATT GGGAGCCAAG 
AACTCACCCC GTCCACCGCG GCCCATGGTA CAGATGTGCA TCGCTCTGGC TGAGCTGACA 
CCACGCGGTC TCGATGCCGA TTACGACGTT CAGGTGGAGT CCTTTTACCG ACCCTACCAT 
GACTTTATCG ATGTCATGAT GCGCATGTGC GTCAACACGG GCACTCTGAA CGACACGCTA 
GTCAAGCTGC AATGCTTGGT GGCCATCGAG TCCACGCCGC TGCACTTCAC CTATTTCCCC 
AAATTCTGGG TGGGCATACA CAACAATGCT CTGACGCACA AATACGTTGA GCTGCTGGTG 
AAAAATCAAC TGTTGGTAGA GTACCTTCAC AATGTCCTTA GGGACGAGCG AAGCATGTTG 
AAGGATGCCT GCGTTCGTGA ATTTCTGGAG CTCTATTATC ACAAGGTTGC CGCCCAGCTG 
CCAGTGGCCC GTATGATCTA CACAATCAAC TATGGTATGC ACTCCAAGGA CGACATTGAT 
GAGCTGTGTG GCGATCTGTT CGCCATCCGA ATCATTGCCC AGGCCACTGG AGTGCCGGCC 
TCAGTGCGCA AGGAGTTGCG AGGATCGCTG AGGGCGCTTC AGAACAAGAG CGAGCGGTTT 
AGGAAGGAAT CGGAGAGGGA TCCATTTCCG AACAAGAAGC AGAAACGTGA CTCGCAAAAG 
ATCAAGGAAA AGGAGCACCC TCAGCCGGAG AGCGAGAAAG AGACCTCAAC GGAGAACGAC 
AAACCATCAG ATGTTTCCAT GGAATCCAGC GGTAACGCGG AGCAAGCAAC GGACAGCACA 
AAACCACCAA CTCCTGCAGG CAGCGACGAT GAGCAGATGG ACAAAACTTC AGTTCCCTCG 
TCCGATGACG AGACAGAGTT GGAGGACGAG CTGCAGCCGA CGCCCAAGCG CAACAAGAAG 
GCTTCGAATA AGAAGACCGC CCAGGATCGT GTCAACGAGG AGCGCGAGAA GCGACTTATC 
ACGCTGATCA CCATGGAATC ATATATCCAG AGCATTTTTG CCATCCTTAA GAGAGATGCC 
AGTGTCCCCA GTTCGGGAGC GACTAAGGAG CCCAGTGAGG AACCGTGTGG CGAGGCATCC 
ACTTCGGCGG CTGCCGCTGT AAAGTTATCT CGTCCCAAAG GAGCCTGTAC TCCGCCGGAG 
CCAATTACCG ACGTAAACGA TACGCGTTGC AACATAGATA CTGAGACAGA GGCGGAGGCG 
GATGAGCAAT CTCCAAAGAC GAGCCAGACA AATGGTAGCC AACAGAACGA ATCCCCGCCA 
GCGGCGACCA GTGCAGACAC TGCACCCGCG AATCCTTCAC CAGCTCCCGC GGCCGCCGTT 
GCGTCCACAT CCCAGGCGGC AAGTCCAACA CAGATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

puf ( NM_143018.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651275.1 Drosophila melanogaster puffyeye (puf), transcript variant D, mRNA.

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CloneID	OFa05978
Clone ID Related Accession (Same CDS sequence)	NM_143018.4
Accession Version	NM_143018.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	puffyeye, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_143018.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCGAGG TGTGCGCCGA CTTCCAGAAT CTCCTGGAGC TCTATGAGGT GCGCGTGGCC 
AGCTCGGATC TGAAGTTCCA GCTGCTGCTG AAGAGCGAGA TCGAGACCAC CTTCAACTAC 
ATCCAGTCGT GGCCGCAGCG TCAGTGCATG TGCCTGTACC GGGACACCAA GAACTATGAC 
CGTTTCAACC TGGTAGTGCA GTCGCTCATC TGTCTGACGG TGCAGCATCT GAAGCACATC 
GACCACCTCA TCGACAACTA CAAGCGGCTA ACGGCCAGTG CTGCCCAGGT GGTGGCCCAG 
GCCCAGCAGC AGCGTGAACA GCAGCGGGAC GAAGCGTCAG CCCAGGCAGA GGCAAAGGAA 
TCATCCGCGC CAGCGGAAGA ACCTAAGAAG GAGGAGCCAT CTGGGTCCGC CGGTGAGGAG 
GCTCAGGGCT CCGGCGACGG GCCAGCCAAA AAGCCGCCAG TTGGCCCCTG CACTCCCCCT 
CCCCCGCAAA CGGCCAATCC TCAGCACAAA TCCCACCTGC AGTACACAGA GGAGCCCTGG 
ATCCTGCCCG AGGTGGAGAA ACTGTTGGTG CTCGTTTCAA AGGTGTTCCT GCTCAACTTC 
CCGCTGTACA TCGCCCACAA ACATGGCATG CACTCTCGGC TGGACGACCT GCAGGCTGAG 
GAGGCCCACC ACCTGGCCCT CATCTGCGAT CTTCACGACA ACGACCTGCC CATCTATCTG 
CTGCGGAATG TGAGCCTGTT CTGCAACTCG GGCGGCTTTG GCGCCATGTC GCTGTGCTTC 
GAACATCCCG ATCTGCCAGT GTCCACCGCT CACTCGATGA CCGCTGCCGT CTCAAATGTG 
AAACTTTGGC TGAACTATCA CTGTAATACT CAGCTCTTCG TGCCGCTGCG AAGTCGGATA 
CTGCAGTACA TGTGCAAGCT GTCGGATCAG AGCCTCCGAT CTGCCGCAAC CCGCGCGATG 
GCCGATTTTG TGTGGTCCTC TATGCGAGAC CCACTGGATG TTGCTGTCAA CTTCGACACC 
GAAGGATTGG CCCTGGCCTT TAAGTACTTC ACATCGACCA CGCTAACCAT GCGACTGGCA 
GGCATGGCCC AGATCAACGC ACACATTAAT CTATTTAACG AGATCTGCAC CACCGAAACG 
GTCAACGAAG TGGAGCTGTT CGGGCAGCGC ATCGCGAACT GGCTGACTGA AAATCATATT 
GTACAGCACC TGTTTGGCCC GAATCTGCAC GTAGAAATCG TTAAACAGGC ACACGTGCTG 
CTCAACTTTT TGGCGGTAGA GAACCAGATC TCTGAAGAGG ACATCAAGCT GATCTGGCAA 
GCCACCCAGC TGAAGCACTG CTCAAAGACG ATCTTCGACA TCCTGCCTTC GCTGGTGAAG 
AATCTGACCC CGCGCCCGGC AATGCACCTT TACTCCCTCC TTTGCCGCAT GGATCCCAAG 
GAGCACACTG AGCAGAGCAT ATACATAGCC TCAGCGCTAA CAAAACAGCT GTGGACTCGT 
GATACCTCAC GCAGTCAGAT GAACCTGATG CAAGACCACC TGCTGGGGTC CAACGTCACC 
GCTTCCAGCT CTGACAGCGG CAGCATTGAG GGCAGCAACA CAGAGGACGA CCACGTGGGC 
GCCGACGACA GTAGCATTGC CAGCGGTGGC GGCGGCGGAG GTGTTGGTAA CAAGAGTCCA 
ATCGATGGAG TTACTCCATG CAAGCAGGCC CGGCACAGGC GGCACATCTG TGACCCCACC 
ACCGAGAAGG GAAAGCAAAT AAGTCCCGAG GATATGGCCA AAGTAGACTT GGTGAACAAA 
CGGATTGTGA ACATTATCGA CAACACAAGC AGTGAAGAGG AGCTGCAATC GCGGGCCGCA 
CTGGAGCTGC AACTGCATCG CAGCCGCAAA AAGACGAGCA ACAAGCGACG GCGACAAAAA 
ACCAACAAAC AGATCATTCT GCCCCATGAG CTGGTCGAGA TTTGGGACGG AGTTGAAGAT 
GTGCCCAGCA GTGACGAGGC TGATGGCGAA GCCGATGGTG ATGGCGAGGG CGAACTTTTA 
GCGGACAGCG ATGAGTGCAG CGATGGTGCT ACGAGTCAGC TGGTACCCGA CGCCGTGCTA 
AAGCATCTGC AGGGAGAGGG ACCTTTTATC AGGGCCATCG AGACCAATAT CAATGAGCTG 
CTCAGTGGTG CCGAGAACGA TGGCAGTTAC TCATCCCCGA TGAGTAACAA ATCTGAAAAG 
AACCTGGCTG ACTTCGATGA CGAGGACGTG TCGCCTTGCG AGGAGGAGCT GGCCCAGCTG 
GTCAGCTCCC GCGCCAATTG TTCGGACGTT CCACCAGCCT TTGCAGCCGC TGCCGCCGCC 
ATGATGGTGG CCCAGTCGGC GATGGCCCTT CAGAAGTCTG GAGAGTCGAA TGTGGCTGCT 
GCTGCCGCTG CTGTGGCCGC TGCTGCAGCG GCTACCGGAA CTGCACAGCA AACGCTGGTG 
GCAATGAACC GGCAGGCCAG CGTTGCTGCA GCTGCCGCCG TGGTTGCTGC GGCCAAGGCA 
AAATCGGACT CAGATGTCGA TCTGATGGAT GTGGTTTCCG GCGGAAAGCA GCACCATTCA 
CCAAAGGCGA GCCAGGGCAG CTCTACGTCC GGGAGTACAC CGGTGCAGCC CTCGTTCAAG 
CTAAATGATG TCTGCCAGCC CGGAAACACA CTGCTTTGGG ATCTGCTTCA AGACGATAAA 
ATTGGCCAAC TAGGCGAGTC TCTGGCTCTA GAGGCGGAGA AAGCTCTGGC CACTCTCCTG 
TGCTTCAGCA TGGACCGCCA ACTGCGAACG AAATTCATCG AGGGTTGTCT GTATAACGTC 
GCCAACAACC GGAGTGTTAT TGTCAGTCTG CGACTGCTGC CCAAACTGTT CGCCTCGTTC 
CAACAGTTTC GACCCTCGGA CACGCACTCT ATGACGATGT GGGCAGAGCG AAATCACCGG 
ATGATGCAGT GCTTTTTCAA TAATATACGT CACTATGCGC GTCGCCATGC CGAGGTACTG 
ATCACCCAAA ACGGAGAGCA GCAGCAGCAG CAGCTGGGCG GACAACTCTA CTCGCACAAG 
ACACAAGTTT CTGTAAGACT GCAGTTCCTA TCATCGATAT TCTCGACGGT GGGATCCCCG 
AAAAGCTTCC GCCTAACGCT GGAGCAGCTT GACGCGCTAT GGGAGTGGCT GGCCCACGAT 
CCGGAGTGTG CCGACTGCTA CTTTTCGTGG CTGCAGGCCC AGGCCAAAGG CGGTGATCAG 
CATGCTCTGG GCATTGAAGC ACTTCAGCAT TTGTATTTGA AGAAACTCCC AGAGCTGCGA 
CCCGAGGAGT TCTCTATGGT TGCGTTGGGA CTATTTCAAC AGCTCTGTAG CTTCGCCCGC 
ATCGCCATGG CCGAGTACGA CAATCATAGC GACCAGATCT CGGCGAGCGC CAGTGCCGTT 
GGCATGTACC ATCTTTGGAA GATAGCGTTG CGTGCCCAGA GCAACGATGT CTCCTTGGCG 
GCCATACAGT ACATCAACAT GTACTACATG GGTCAACAAT TGCGCCTGGA GAAGGAGTTC 
GTTTCACAGT GTATGGAAAA CCTAGTTCAA GCGGCCACGG CTTTGGAAAG CATCGATGAT 
GAGAATGCTT TGATGCGTGT CCAGCGTGGC CTGCTCTTGC TGAACACCCA TTTGGATACG 
TTCCGGCGTC GATATGCGTT TCATCTGCGC CGCTGGGCCA TCGAAGGCAA AGGGATTGGA 
TCGCACAGTA ACCTTAAAAA TGAGGGAGCG GGTCCACCAC TACGAATAGT GCTGCAACAG 
GCAGGACTTT CGGAAAAAAG CCTTTTGCAG ATGCACGCGT GTGACTTAAT CGCCGACCTC 
AAGGCTGAGG TGTCCAAGTG GTGGGAGAGC CTTCAAACTG GACTAGCTGC TCCTGTGCTC 
GGTCTGCTGC TCAGCGATGG ACCGCTACGC ATTATCACAC AGGGACAGGA ATTGACCTCG 
GATTACGATG AACGGAGTCT CGGAGATGCT GGTTTCAAGG ATAACCAGAT CGTCTACGTA 
TCGTTAGGTG GACGTGGAGC CAGGCGAAAG GAATCCAATT TGGAGCATCC GTCAATGCTG 
CCGCCCCCAC CAAAGGAGTG TTTGCCCACG GTGCTCCTGC TGCAGCCAAA GTACTTCGAG 
AAGCTATTTT GCCTGATGCA GACCCTGGGC GATATGCAAC CGCAAGCGTC GACTGTAAAT 
CCCCAGCACC ACACAAAGGC GCAATTGCTC TCGCGACGCG TATGGGATAT CCTCGCCATG 
CTGCCAACTA ATCCGCACAT TCTAGACGCC TTTAAGAGTC TGGTTACGGA CCTAAGCGAA 
CTCGAGCAGC TTGATGCTGG CGGAGAGGAG GAGCAACTGG CCACCAAGCG CAAGCAAATC 
AAGCAGAAGT TCCGAGATCT TCTCGACCCG AACAACTTGC AAAAGTTCAT GTACTCCCTC 
CATATTGTGG AGAGTCTGGC CCTAACAAGT TCCAGACGTG GTGAATCCAA CGGCAATGTG 
GCCATGGGAA ATACACCGGA ACAGGTACGG GTGAAGAAAT CCTCGATGGG AAGACGGAGA 
AATAGCAACG AGCAGCCGCC ACCGCCGCCG CCAGAAGTCA AGATGAGCAA GGAGCAACTG 
TGCGAGCTGG AGGCCCCACT GACGCCCACA CCCTCGACTG GCTTGCAGGA TGTGGAAACG 
GAGGCCAGCA GTAGCAGCGG TGGCGACAAA GAAAACCAAC CGAAACAACA CAGCAAGCGT 
CAGAAGAAAG GCGAAACTTT TGAACAGGAA AAGGAGCGCC CAGTGGGTTG CTCCACGCCG 
CCGTCTCCAA CTCCTCCGCC ACCAGCACTT TCGGTAGTGG AAAGAGGTGA CAATAAGTGG 
AGTGAGGCGT TCGTCAAATG TGGTGGCCTT CGTCATCTGT ACGAAATCTT TAGTGAGGGC 
CAACTACAAC AAAGCGCACA TCCCAAGGAG TTGGCTTTAA ATGAATGGCG ACACGATTGC 
CTGGCCAGCC TGCTGCGCAT TCTCTGGCTT CTTGGATTCG AGGAACTGCA GTCGGCGGAT 
GCCCATGTTC TAATGTCACG TCCGCACCCG TTTATGCTAC AGTTGATGGA GGTACCACAG 
TGCCTGACCC GACTCTCATC GATCCTTAAC GACGAAGTTC ACCAGCAGCA TCAGGCTTCT 
TCGGCCAATC CACTGGTATT CCCCTATCAG TTCCAGCACT TGCGTACTGG CTTTTGGGGC 
AGGGCCCAGC TCATCCAGTT CGCTATGAAC ATTCTAGTGA GTTTTGTGCA CGCCTCAGCG 
GAAGCGAGAA GGCTTCTCTG GGCCCCAACT GGTACCGATC ACTGTCGATG GCTGCAGAAG 
TTCATCCTGG AGGATCCCGA ACCGGCGGTA CGGCGTGAGA TATGCGCTGG TCTTTATCGC 
ATTTGCTTAG GAAACGCACA TAGCTATCGC CTCTTACTAG CACCACTGCT GCACAAGCTC 
ATCGCCCTGT TGCCATTGGC CGAGCAGATG AGCTCTGGCA ACCAGCACAC GCAGTTTTTG 
CTCTCGGAAG AGGGCAAGGA TCCCTACGGA CCCGCCTGCC GTGACTACTT TTGGCTGCTG 
GCCAGATTGG TGGATACTCT CTCCCCGGAA ATGGTGGCCG AGGAGCACAT CGACATTGAA 
ATGCTGTGCG AAAGCATCTC GCAGAGCATT CTCACCCGAG AGTATTATGA ACTGCGCCAT 
GGTTACCAGG ACGATGGCTT GGTGGGTCTC CTAAATCTCA TGTCCAATTT GATCAAGTAC 
GACACCACAT TCAAGTACAC ACCCAAGGCG TTATCCTTTA TTGAGCAACT GATTGGCTTC 
CTGTTTGACA TGCCCTCGCC GGCAGATCGC CAGAAGCCCA AGTGCAAGTC GGCGTCGTCT 
CGAGCATCTG CCTACGATCT GCTGGTTGAA CTTTGCCGTG GATGCGCCAC AAACTATGCA 
TATCTGCATG GACGTCTGTT GGCACAGCAC AAATCCGGAC CCAAGCAGCC GTATCCCTGG 
GACTATTGGC CGCGCGATGA AGGCAGAGCT GAGTGCGGAT ATGTGGGGCT AACTAATTTG 
GGAGCCACTT GCTATATGGC CTCTTGCGTC CAGCACTTGT ACATGATGCC ACAGGCGCGA 
GCAGCTGTTT TGCGAGTTCC ACCCAACGCC GCCCGTAAGC ATGGGCCCAC ACTTCTGGAA 
CTACAACGGA TGTTTGCCTA CCTACTGGAG TCGGAACGGA AGTCTTACAA CCCGCGAAGC 
TTCTGCCGGG TGTACCAGAT GGATCACCAG CCGCTAAATA CGGGAGAGCA AAAGGATATG 
GCAGAGTTCT TTATCGATTT GGTGTCCAAA CTGGAAGATA TGACGCCTGA CTTGAAGCAC 
CTGGTGAAAA GGCTCTTCTG TGGATCCTTG AGCAATAATG TCGTCTCCCT GGATTGCGGT 
CACGTTTCCC GCACGGCCGA GGACTTTTAC ACGGTTCGCT GCCAGGTTGC AGATATGCGC 
AATCTGCAGG AATCCCTGGA CGAAGTCACC GTTAAGGATA CCCTCGAGGG CGACAACATG 
TACACCTGCT CGCAGTGCGG CAAAAAAGTA CGAGCCGAGA AGCGGGCTTG CTTCAAGAAG 
CTTCCCCAGA TCTTGTGCTT CAACACCATG CGCTACACCT TCAATATGGT CACCATGCTC 
AAGGAGAAGG TCAACACGCA CTTTTCCTTT CCGCTACGCC TGAACATGTG TCACTATGTG 
GAGAAGACCT TGATGCCGCA GCAGTACAAA GAGGAGCGAG AAAGGCGCCA AAAGGAGAAG 
GAAGGAGCAG ATGGTAGTGG CGATGGGAAT GACAACGAAA AGGCAGAGGC CACGCTAGAT 
GACGATATTG AAGAATGCTA CGAGTACGAA CTGGTGGGCG TCACTGTCCA CACGGGCACA 
GCGGATGGCG GCCACTACTA CAGCTTTATA AAGGAGCGGA CGAAGACTAG TTACCATACG 
CACGAGCGGT GGTTTCTTTT CAACGATGCC GAAGTGAAGC CTTTCGATCC CTCACAGATA 
GCGGCGGAGT GCTTTGGCGG CGAGATGACG AGCAAAACCT ACGACTCAGT GACGGAGAAA 
TATCTGGACT TTAGTTTCGA GAAGACCAAC TCTGCCTACA TGCTGTTCTA CGAGCGCCGA 
CTGCCAGAGC ATCTGCAGCG CCGGCATTCC GAGCTGCTGG TTACACCCAC GCCCAGTCCC 
ACAGTGGAAG AGAAGTCCGA GGCGGAGGAG CCCACTAAAA TGGAGACAAG CTCTAGCGAA 
ATCAAGGCAG ATGTGGATGT GGAAGTGGAA GTTGAGGAGA AGGACAAGGA GAAGCCTGCG 
CAGACTGACA CAGAATCTAA GGAAACGCCT GCAAAAGAGG AGATCGCGGA TGACAAATCG 
AAGCAGGATG AGCCTGAGGA AAAGAAAATC GAAAAGCAAT CCAGAGAGGG CGAAGAGAAA 
TCGGAAACGG ACGAAAAGCC CACGGAGATG ACGACCGTGA GCACTGAGGA GGAAAAGCAG 
CCAACAGCCA ATTGTGATAA TCACCAGCAA AACAACAATA GCAACAGCAA AGCCAGCAAC 
GATCAGCAAC CATCCACGTC GAAGGCTGCC CAGAAGCTAC AACTATTTCG TCCATTGTTG 
AACAAGGAGC TCGAGGATTG GATTTGGCAG GACAATCGAC AGTTCCTGCA GGATCGCAAC 
ATTTTCGAGC ACACATATTT CAACTTTATG TGGCAGATAT GTGGGCACAT ACCCCAATCA 
TTAATATCAG AGACGGACGT CACCTGCATG GCAGCCAAGT TGTCTGTGTC CTTTTTCATT 
GAGACTTTTA TTCATGCTAA GGAAAAGCCC ACTATGGTGC CCTGGGTGGA GCTGCTGACC 
AAGCAGTTCA ATGCCAGCCA AGAGGCCTGC GAGTGGTTTC TGTCGCACAT GTCGCAGGAG 
CCCTACTGGC CGGTCCAGGT GCTAATCCAG TGTCCCAACC AAATGGTGCG TCAAATGTTT 
CAGCGTTTGG TAATCCATGT CATCCAGCAA CTACGTGCCT CACATGCTCA TCTGTATCTG 
GAAGTGGAGA CAGACGAGGA TGACAAGGAG TTAATAGGCC AAGCCTCTTG CGTCACCCGT 
TTCATTGGTT CGCTCATCTC GCTCCTGGAG CACGGTGCCC GAGCGAACCT GCGGCACTTG 
TCCGAGTACT TTGGACTGTT GTGCGAGTTC TCACGGATGG GAGATGAGGA AGCCATGTAC 
TTGCTGCGCA TCGGTGTACT CAAGAGCCTG GTCGACTTCT ATCTGGGTCA CAAGCAAACA 
GACAGCATAG ACATAAGCTC TGACAACGAA GACAACTCCT CTGAAGAGGC GCTGTCCGTG 
GAGAAGATGC GTCCGGCCTC GCTGGACAAG ATGATTGCTC TGTGCGCCAG CCTAGTGGAA 
AGATCAAGAG GAGCCGACTT CCGGCTGCGA CTCTCGCCCA AAGACTTCTC TGCAATCGCC 
GGCGGCAAGG GTTTTCCATT TTTGTACCAA CAAATAAAGG ACGGCATTAA TCCGCACCAG 
ACAAAGCATT TAATACACGC GCTTTGTCGC TGGGATGAGC GCCTGGCCAC GCAGATCATT 
GGCATGCTCT TCGCGTCGGT AACCAAGCAC ACGGAGCTGT GCGCGCCATT CTTCAAGCTG 
CTAACTCTGC TCACCGAGAC GCAGGGCGGT CCAGTGGGCC TGCCTTGTTT CACCCAGTTG 
ATCCTGCCAC GAATGTGGGA CGCCGCCGAG TACTGTCCAC AGTCGGTGCT CGACTGGCTC 
TCGCTGCAGG CCACCAAGAA CAAGATCGCC CACGCCTGGA TACTTCAATC GGCCGAGAAG 
TGGCTCGAGC AATTCCTGCT GGCCCACGAT AACACTCGTG TTCGGAACGC TGCCGCCTTT 
CTGCTGGTGG CACTGGTACC CTCCCAGCCA TTCCGGGCCA ACTTCCGCGC CCACTCGCAA 
CACAAGCTGC TGGCCCTCAA TCCGCACAGT TATCGCGACA TAAATAGCGA TGCCCAGGCG 
GTGCTGCACC AGGTGATCAC GCTGCTCCTG CGATTGCTGC GTCCAGCGCG CGTCTATGCG 
GATATCGGAG CCCATGGCAC GACCAAGCTG ACCGCGTACT TTAATCTGCT CAGCTATTGT 
ATGGTTTCAA AGACTGAAAA ACTAATGGCA AGTTCGTACA TGCGCTCCCT GTGGGAGCTG 
TTCCATCCAC GCCTCTCGGA GCCCAGTGTT CCGGCCCATC ACAATAAGCA CGCACTATTA 
ACCTTCTGGC ACCACTCACT GGTGGACTGT CCGGAGAATG CTGCCCAGGT GGCCAACTGC 
CCGGAGATTA CGCGCAACAT TGCATTTAAC TATATACTGG CCGATCATGA TGACGCGGAG 
ATAGTCACCT ACAACCGTTC CATGCTGCCC GCCTATTATG GACTACTTCG TCTGTGCTGC 
GAGCAGAGCC GTGCACTCAC ACGCCAATTG TCCCAGCATC AGAATCTTCA GTGGGCATTT 
AAAAACATCA CACCGCATCC CACGCAGTAC GCGGCCGCCG TGGACGAGCT TTTCAAGTTG 
ATGGCTCTGT TCGCCACCCG TCATCCGGAT GCTAGTGAAC AGGAAAAGCT TGATGTGACC 
CAGTTTAGGA GGGCTGTGAT TGTGTCCTAC ACCAGTAGTC TGGATGCACG TGTCTCTTGG 
TCCACGCTGA TCAGTGCTCT TAAGATACTC GTGGACAATG AGGAGGACCG CACGATGGTG 
ATCTTCAACG GCGGCATTGA GATGTGTTTC GAGGCGCTGC ACACGCTACA TTCCATGCAC 
CACGAGGCCA CCGCCTGCCA CGTGGCCGGA GACCTATTCG ATCTCTTGGG CGAGATGCTA 
CTCCTCCTAG CCACTCTTCG AACCCGCACA GACAGTCCTG CTCAAAAGAA GCAGCAGCAA 
CAACAGCAGC AGCTGGAGCA ACAGTTGCAG TTGCAGCAGC AACAGATGCT GCAGAAACAA 
CAGCAGCAGT CGCAAAGCCA AACGCAAACA CCGCAGTCGC CGCAGCAAAA GGAAAAGCAG 
CTGCAGCAAC AAATGCAGCA GCATCTGCAG CTGCAACAGT TGCAGCAAAT GCAGTTCCAG 
CAGCAACACT TCCTGCGCCA GCAACATCAA CACTCGGCGC TGGCAAAGGC ACTGCCGGAT 
GCAGTGAAGC GACTGGCTAC GCTACTCAAC ACCTTCAATT CGCCAGAGAT CAGTCGCATG 
GCCTTGGAGG TGCTCAAGGA GCTGGTGCGC AATCCCAGCC TGGAAACCAT TAGTATTCTG 
GCGCCCATTC TTATCAACTG TCATCTGTCC GTGGCGAATG CTCCCAATGC GATCGGTCCT 
CTGGGACCGT ACTTTCCGCG TCGCGGTGCG AAGCATACGC CTTGGCCATT GGGAGCCAAG 
AACTCACCCC GTCCACCGCG GCCCATGGTA CAGATGTGCA TCGCTCTGGC TGAGCTGACA 
CCACGCGGTC TCGATGCCGA TTACGACGTT CAGGTGGAGT CCTTTTACCG ACCCTACCAT 
GACTTTATCG ATGTCATGAT GCGCATGTGC GTCAACACGG GCACTCTGAA CGACACGCTA 
GTCAAGCTGC AATGCTTGGT GGCCATCGAG TCCACGCCGC TGCACTTCAC CTATTTCCCC 
AAATTCTGGG TGGGCATACA CAACAATGCT CTGACGCACA AATACGTTGA GCTGCTGGTG 
AAAAATCAAC TGTTGGTAGA GTACCTTCAC AATGTCCTTA GGGACGAGCG AAGCATGTTG 
AAGGATGCCT GCGTTCGTGA ATTTCTGGAG CTCTATTATC ACAAGGTTGC CGCCCAGCTG 
CCAGTGGCCC GTATGATCTA CACAATCAAC TATGGTATGC ACTCCAAGGA CGACATTGAT 
GAGCTGTGTG GCGATCTGTT CGCCATCCGA ATCATTGCCC AGGCCACTGG AGTGCCGGCC 
TCAGTGCGCA AGGAGTTGCG AGGATCGCTG AGGGCGCTTC AGAACAAGAG CGAGCGGTTT 
AGGAAGGAAT CGGAGAGGGA TCCATTTCCG AACAAGAAGC AGAAACGTGA CTCGCAAAAG 
ATCAAGGAAA AGGAGCACCC TCAGCCGGAG AGCGAGAAAG AGACCTCAAC GGAGAACGAC 
AAACCATCAG ATGTTTCCAT GGAATCCAGC GGTAACGCGG AGCAAGCAAC GGACAGCACA 
AAACCACCAA CTCCTGCAGG CAGCGACGAT GAGCAGATGG ACAAAACTTC AGTTCCCTCG 
TCCGATGACG AGACAGAGTT GGAGGACGAG CTGCAGCCGA CGCCCAAGCG CAACAAGAAG 
GCTTCGAATA AGAAGACCGC CCAGGATCGT GTCAACGAGG AGCGCGAGAA GCGACTTATC 
ACGCTGATCA CCATGGAATC ATATATCCAG AGCATTTTTG CCATCCTTAA GAGAGATGCC 
AGTGTCCCCA GTTCGGGAGC GACTAAGGAG CCCAGTGAGG AACCGTGTGG CGAGGCATCC 
ACTTCGGCGG CTGCCGCTGT AAAGTTATCT CGTCCCAAAG GAGCCTGTAC TCCGCCGGAG 
CCAATTACCG ACGTAAACGA TACGCGTTGC AACATAGATA CTGAGACAGA GGCGGAGGCG 
GATGAGCAAT CTCCAAAGAC GAGCCAGACA AATGGTAGCC AACAGAACGA ATCCCCGCCA 
GCGGCGACCA GTGCAGACAC TGCACCCGCG AATCCTTCAC CAGCTCCCGC GGCCGCCGTT 
GCGTCCACAT CCCAGGCGGC AAGTCCAACA CAGATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651275.1
CDS	358..12096
Translation	MCEVCADFQNLLELYEVRVASSDLKFQLLLKSEIETTFNYIQSWPQRQCMCLYRDTKNYDRFNLVVQSLICLTVQHLKHIDHLIDNYKRLTASAAQVVAQAQQQREQQRDEASAQAEAKESSAPAEEPKKEEPSGSAGEEAQGSGDGPAKKPPVGPCTPPPPQTANPQHKSHLQYTEEPWILPEVEKLLVLVSKVFLLNFPLYIAHKHGMHSRLDDLQAEEAHHLALICDLHDNDLPIYLLRNVSLFCNSGGFGAMSLCFEHPDLPVSTAHSMTAAVSNVKLWLNYHCNTQLFVPLRSRILQYMCKLSDQSLRSAATRAMADFVWSSMRDPLDVAVNFDTEGLALAFKYFTSTTLTMRLAGMAQINAHINLFNEICTTETVNEVELFGQRIANWLTENHIVQHLFGPNLHVEIVKQAHVLLNFLAVENQISEEDIKLIWQATQLKHCSKTIFDILPSLVKNLTPRPAMHLYSLLCRMDPKEHTEQSIYIASALTKQLWTRDTSRSQMNLMQDHLLGSNVTASSSDSGSIEGSNTEDDHVGADDSSIASGGGGGGVGNKSPIDGVTPCKQARHRRHICDPTTEKGKQISPEDMAKVDLVNKRIVNIIDNTSSEEELQSRAALELQLHRSRKKTSNKRRRQKTNKQIILPHELVEIWDGVEDVPSSDEADGEADGDGEGELLADSDECSDGATSQLVPDAVLKHLQGEGPFIRAIETNINELLSGAENDGSYSSPMSNKSEKNLADFDDEDVSPCEEELAQLVSSRANCSDVPPAFAAAAAAMMVAQSAMALQKSGESNVAAAAAAVAAAAAATGTAQQTLVAMNRQASVAAAAAVVAAAKAKSDSDVDLMDVVSGGKQHHSPKASQGSSTSGSTPVQPSFKLNDVCQPGNTLLWDLLQDDKIGQLGESLALEAEKALATLLCFSMDRQLRTKFIEGCLYNVANNRSVIVSLRLLPKLFASFQQFRPSDTHSMTMWAERNHRMMQCFFNNIRHYARRHAEVLITQNGEQQQQQLGGQLYSHKTQVSVRLQFLSSIFSTVGSPKSFRLTLEQLDALWEWLAHDPECADCYFSWLQAQAKGGDQHALGIEALQHLYLKKLPELRPEEFSMVALGLFQQLCSFARIAMAEYDNHSDQISASASAVGMYHLWKIALRAQSNDVSLAAIQYINMYYMGQQLRLEKEFVSQCMENLVQAATALESIDDENALMRVQRGLLLLNTHLDTFRRRYAFHLRRWAIEGKGIGSHSNLKNEGAGPPLRIVLQQAGLSEKSLLQMHACDLIADLKAEVSKWWESLQTGLAAPVLGLLLSDGPLRIITQGQELTSDYDERSLGDAGFKDNQIVYVSLGGRGARRKESNLEHPSMLPPPPKECLPTVLLLQPKYFEKLFCLMQTLGDMQPQASTVNPQHHTKAQLLSRRVWDILAMLPTNPHILDAFKSLVTDLSELEQLDAGGEEEQLATKRKQIKQKFRDLLDPNNLQKFMYSLHIVESLALTSSRRGESNGNVAMGNTPEQVRVKKSSMGRRRNSNEQPPPPPPEVKMSKEQLCELEAPLTPTPSTGLQDVETEASSSSGGDKENQPKQHSKRQKKGETFEQEKERPVGCSTPPSPTPPPPALSVVERGDNKWSEAFVKCGGLRHLYEIFSEGQLQQSAHPKELALNEWRHDCLASLLRILWLLGFEELQSADAHVLMSRPHPFMLQLMEVPQCLTRLSSILNDEVHQQHQASSANPLVFPYQFQHLRTGFWGRAQLIQFAMNILVSFVHASAEARRLLWAPTGTDHCRWLQKFILEDPEPAVRREICAGLYRICLGNAHSYRLLLAPLLHKLIALLPLAEQMSSGNQHTQFLLSEEGKDPYGPACRDYFWLLARLVDTLSPEMVAEEHIDIEMLCESISQSILTREYYELRHGYQDDGLVGLLNLMSNLIKYDTTFKYTPKALSFIEQLIGFLFDMPSPADRQKPKCKSASSRASAYDLLVELCRGCATNYAYLHGRLLAQHKSGPKQPYPWDYWPRDEGRAECGYVGLTNLGATCYMASCVQHLYMMPQARAAVLRVPPNAARKHGPTLLELQRMFAYLLESERKSYNPRSFCRVYQMDHQPLNTGEQKDMAEFFIDLVSKLEDMTPDLKHLVKRLFCGSLSNNVVSLDCGHVSRTAEDFYTVRCQVADMRNLQESLDEVTVKDTLEGDNMYTCSQCGKKVRAEKRACFKKLPQILCFNTMRYTFNMVTMLKEKVNTHFSFPLRLNMCHYVEKTLMPQQYKEERERRQKEKEGADGSGDGNDNEKAEATLDDDIEECYEYELVGVTVHTGTADGGHYYSFIKERTKTSYHTHERWFLFNDAEVKPFDPSQIAAECFGGEMTSKTYDSVTEKYLDFSFEKTNSAYMLFYERRLPEHLQRRHSELLVTPTPSPTVEEKSEAEEPTKMETSSSEIKADVDVEVEVEEKDKEKPAQTDTESKETPAKEEIADDKSKQDEPEEKKIEKQSREGEEKSETDEKPTEMTTVSTEEEKQPTANCDNHQQNNNSNSKASNDQQPSTSKAAQKLQLFRPLLNKELEDWIWQDNRQFLQDRNIFEHTYFNFMWQICGHIPQSLISETDVTCMAAKLSVSFFIETFIHAKEKPTMVPWVELLTKQFNASQEACEWFLSHMSQEPYWPVQVLIQCPNQMVRQMFQRLVIHVIQQLRASHAHLYLEVETDEDDKELIGQASCVTRFIGSLISLLEHGARANLRHLSEYFGLLCEFSRMGDEEAMYLLRIGVLKSLVDFYLGHKQTDSIDISSDNEDNSSEEALSVEKMRPASLDKMIALCASLVERSRGADFRLRLSPKDFSAIAGGKGFPFLYQQIKDGINPHQTKHLIHALCRWDERLATQIIGMLFASVTKHTELCAPFFKLLTLLTETQGGPVGLPCFTQLILPRMWDAAEYCPQSVLDWLSLQATKNKIAHAWILQSAEKWLEQFLLAHDNTRVRNAAAFLLVALVPSQPFRANFRAHSQHKLLALNPHSYRDINSDAQAVLHQVITLLLRLLRPARVYADIGAHGTTKLTAYFNLLSYCMVSKTEKLMASSYMRSLWELFHPRLSEPSVPAHHNKHALLTFWHHSLVDCPENAAQVANCPEITRNIAFNYILADHDDAEIVTYNRSMLPAYYGLLRLCCEQSRALTRQLSQHQNLQWAFKNITPHPTQYAAAVDELFKLMALFATRHPDASEQEKLDVTQFRRAVIVSYTSSLDARVSWSTLISALKILVDNEEDRTMVIFNGGIEMCFEALHTLHSMHHEATACHVAGDLFDLLGEMLLLLATLRTRTDSPAQKKQQQQQQQLEQQLQLQQQQMLQKQQQQSQSQTQTPQSPQQKEKQLQQQMQQHLQLQQLQQMQFQQQHFLRQQHQHSALAKALPDAVKRLATLLNTFNSPEISRMALEVLKELVRNPSLETISILAPILINCHLSVANAPNAIGPLGPYFPRRGAKHTPWPLGAKNSPRPPRPMVQMCIALAELTPRGLDADYDVQVESFYRPYHDFIDVMMRMCVNTGTLNDTLVKLQCLVAIESTPLHFTYFPKFWVGIHNNALTHKYVELLVKNQLLVEYLHNVLRDERSMLKDACVREFLELYYHKVAAQLPVARMIYTINYGMHSKDDIDELCGDLFAIRIIAQATGVPASVRKELRGSLRALQNKSERFRKESERDPFPNKKQKRDSQKIKEKEHPQPESEKETSTENDKPSDVSMESSGNAEQATDSTKPPTPAGSDDEQMDKTSVPSSDDETELEDELQPTPKRNKKASNKKTAQDRVNEEREKRLITLITMESYIQSIFAILKRDASVPSSGATKEPSEEPCGEASTSAAAAVKLSRPKGACTPPEPITDVNDTRCNIDTETEAEADEQSPKTSQTNGSQQNESPPAATSADTAPANPSPAPAAAVASTSQAASPTQI

Target ORF information:

RefSeq Version	NM_143018.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster puffyeye (puf), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143018.4

ORF Insert Sequence:

ATGTGCGAGG TGTGCGCCGA CTTCCAGAAT CTCCTGGAGC TCTATGAGGT GCGCGTGGCC 
AGCTCGGATC TGAAGTTCCA GCTGCTGCTG AAGAGCGAGA TCGAGACCAC CTTCAACTAC 
ATCCAGTCGT GGCCGCAGCG TCAGTGCATG TGCCTGTACC GGGACACCAA GAACTATGAC 
CGTTTCAACC TGGTAGTGCA GTCGCTCATC TGTCTGACGG TGCAGCATCT GAAGCACATC 
GACCACCTCA TCGACAACTA CAAGCGGCTA ACGGCCAGTG CTGCCCAGGT GGTGGCCCAG 
GCCCAGCAGC AGCGTGAACA GCAGCGGGAC GAAGCGTCAG CCCAGGCAGA GGCAAAGGAA 
TCATCCGCGC CAGCGGAAGA ACCTAAGAAG GAGGAGCCAT CTGGGTCCGC CGGTGAGGAG 
GCTCAGGGCT CCGGCGACGG GCCAGCCAAA AAGCCGCCAG TTGGCCCCTG CACTCCCCCT 
CCCCCGCAAA CGGCCAATCC TCAGCACAAA TCCCACCTGC AGTACACAGA GGAGCCCTGG 
ATCCTGCCCG AGGTGGAGAA ACTGTTGGTG CTCGTTTCAA AGGTGTTCCT GCTCAACTTC 
CCGCTGTACA TCGCCCACAA ACATGGCATG CACTCTCGGC TGGACGACCT GCAGGCTGAG 
GAGGCCCACC ACCTGGCCCT CATCTGCGAT CTTCACGACA ACGACCTGCC CATCTATCTG 
CTGCGGAATG TGAGCCTGTT CTGCAACTCG GGCGGCTTTG GCGCCATGTC GCTGTGCTTC 
GAACATCCCG ATCTGCCAGT GTCCACCGCT CACTCGATGA CCGCTGCCGT CTCAAATGTG 
AAACTTTGGC TGAACTATCA CTGTAATACT CAGCTCTTCG TGCCGCTGCG AAGTCGGATA 
CTGCAGTACA TGTGCAAGCT GTCGGATCAG AGCCTCCGAT CTGCCGCAAC CCGCGCGATG 
GCCGATTTTG TGTGGTCCTC TATGCGAGAC CCACTGGATG TTGCTGTCAA CTTCGACACC 
GAAGGATTGG CCCTGGCCTT TAAGTACTTC ACATCGACCA CGCTAACCAT GCGACTGGCA 
GGCATGGCCC AGATCAACGC ACACATTAAT CTATTTAACG AGATCTGCAC CACCGAAACG 
GTCAACGAAG TGGAGCTGTT CGGGCAGCGC ATCGCGAACT GGCTGACTGA AAATCATATT 
GTACAGCACC TGTTTGGCCC GAATCTGCAC GTAGAAATCG TTAAACAGGC ACACGTGCTG 
CTCAACTTTT TGGCGGTAGA GAACCAGATC TCTGAAGAGG ACATCAAGCT GATCTGGCAA 
GCCACCCAGC TGAAGCACTG CTCAAAGACG ATCTTCGACA TCCTGCCTTC GCTGGTGAAG 
AATCTGACCC CGCGCCCGGC AATGCACCTT TACTCCCTCC TTTGCCGCAT GGATCCCAAG 
GAGCACACTG AGCAGAGCAT ATACATAGCC TCAGCGCTAA CAAAACAGCT GTGGACTCGT 
GATACCTCAC GCAGTCAGAT GAACCTGATG CAAGACCACC TGCTGGGGTC CAACGTCACC 
GCTTCCAGCT CTGACAGCGG CAGCATTGAG GGCAGCAACA CAGAGGACGA CCACGTGGGC 
GCCGACGACA GTAGCATTGC CAGCGGTGGC GGCGGCGGAG GTGTTGGTAA CAAGAGTCCA 
ATCGATGGAG TTACTCCATG CAAGCAGGCC CGGCACAGGC GGCACATCTG TGACCCCACC 
ACCGAGAAGG GAAAGCAAAT AAGTCCCGAG GATATGGCCA AAGTAGACTT GGTGAACAAA 
CGGATTGTGA ACATTATCGA CAACACAAGC AGTGAAGAGG AGCTGCAATC GCGGGCCGCA 
CTGGAGCTGC AACTGCATCG CAGCCGCAAA AAGACGAGCA ACAAGCGACG GCGACAAAAA 
ACCAACAAAC AGATCATTCT GCCCCATGAG CTGGTCGAGA TTTGGGACGG AGTTGAAGAT 
GTGCCCAGCA GTGACGAGGC TGATGGCGAA GCCGATGGTG ATGGCGAGGG CGAACTTTTA 
GCGGACAGCG ATGAGTGCAG CGATGGTGCT ACGAGTCAGC TGGTACCCGA CGCCGTGCTA 
AAGCATCTGC AGGGAGAGGG ACCTTTTATC AGGGCCATCG AGACCAATAT CAATGAGCTG 
CTCAGTGGTG CCGAGAACGA TGGCAGTTAC TCATCCCCGA TGAGTAACAA ATCTGAAAAG 
AACCTGGCTG ACTTCGATGA CGAGGACGTG TCGCCTTGCG AGGAGGAGCT GGCCCAGCTG 
GTCAGCTCCC GCGCCAATTG TTCGGACGTT CCACCAGCCT TTGCAGCCGC TGCCGCCGCC 
ATGATGGTGG CCCAGTCGGC GATGGCCCTT CAGAAGTCTG GAGAGTCGAA TGTGGCTGCT 
GCTGCCGCTG CTGTGGCCGC TGCTGCAGCG GCTACCGGAA CTGCACAGCA AACGCTGGTG 
GCAATGAACC GGCAGGCCAG CGTTGCTGCA GCTGCCGCCG TGGTTGCTGC GGCCAAGGCA 
AAATCGGACT CAGATGTCGA TCTGATGGAT GTGGTTTCCG GCGGAAAGCA GCACCATTCA 
CCAAAGGCGA GCCAGGGCAG CTCTACGTCC GGGAGTACAC CGGTGCAGCC CTCGTTCAAG 
CTAAATGATG TCTGCCAGCC CGGAAACACA CTGCTTTGGG ATCTGCTTCA AGACGATAAA 
ATTGGCCAAC TAGGCGAGTC TCTGGCTCTA GAGGCGGAGA AAGCTCTGGC CACTCTCCTG 
TGCTTCAGCA TGGACCGCCA ACTGCGAACG AAATTCATCG AGGGTTGTCT GTATAACGTC 
GCCAACAACC GGAGTGTTAT TGTCAGTCTG CGACTGCTGC CCAAACTGTT CGCCTCGTTC 
CAACAGTTTC GACCCTCGGA CACGCACTCT ATGACGATGT GGGCAGAGCG AAATCACCGG 
ATGATGCAGT GCTTTTTCAA TAATATACGT CACTATGCGC GTCGCCATGC CGAGGTACTG 
ATCACCCAAA ACGGAGAGCA GCAGCAGCAG CAGCTGGGCG GACAACTCTA CTCGCACAAG 
ACACAAGTTT CTGTAAGACT GCAGTTCCTA TCATCGATAT TCTCGACGGT GGGATCCCCG 
AAAAGCTTCC GCCTAACGCT GGAGCAGCTT GACGCGCTAT GGGAGTGGCT GGCCCACGAT 
CCGGAGTGTG CCGACTGCTA CTTTTCGTGG CTGCAGGCCC AGGCCAAAGG CGGTGATCAG 
CATGCTCTGG GCATTGAAGC ACTTCAGCAT TTGTATTTGA AGAAACTCCC AGAGCTGCGA 
CCCGAGGAGT TCTCTATGGT TGCGTTGGGA CTATTTCAAC AGCTCTGTAG CTTCGCCCGC 
ATCGCCATGG CCGAGTACGA CAATCATAGC GACCAGATCT CGGCGAGCGC CAGTGCCGTT 
GGCATGTACC ATCTTTGGAA GATAGCGTTG CGTGCCCAGA GCAACGATGT CTCCTTGGCG 
GCCATACAGT ACATCAACAT GTACTACATG GGTCAACAAT TGCGCCTGGA GAAGGAGTTC 
GTTTCACAGT GTATGGAAAA CCTAGTTCAA GCGGCCACGG CTTTGGAAAG CATCGATGAT 
GAGAATGCTT TGATGCGTGT CCAGCGTGGC CTGCTCTTGC TGAACACCCA TTTGGATACG 
TTCCGGCGTC GATATGCGTT TCATCTGCGC CGCTGGGCCA TCGAAGGCAA AGGGATTGGA 
TCGCACAGTA ACCTTAAAAA TGAGGGAGCG GGTCCACCAC TACGAATAGT GCTGCAACAG 
GCAGGACTTT CGGAAAAAAG CCTTTTGCAG ATGCACGCGT GTGACTTAAT CGCCGACCTC 
AAGGCTGAGG TGTCCAAGTG GTGGGAGAGC CTTCAAACTG GACTAGCTGC TCCTGTGCTC 
GGTCTGCTGC TCAGCGATGG ACCGCTACGC ATTATCACAC AGGGACAGGA ATTGACCTCG 
GATTACGATG AACGGAGTCT CGGAGATGCT GGTTTCAAGG ATAACCAGAT CGTCTACGTA 
TCGTTAGGTG GACGTGGAGC CAGGCGAAAG GAATCCAATT TGGAGCATCC GTCAATGCTG 
CCGCCCCCAC CAAAGGAGTG TTTGCCCACG GTGCTCCTGC TGCAGCCAAA GTACTTCGAG 
AAGCTATTTT GCCTGATGCA GACCCTGGGC GATATGCAAC CGCAAGCGTC GACTGTAAAT 
CCCCAGCACC ACACAAAGGC GCAATTGCTC TCGCGACGCG TATGGGATAT CCTCGCCATG 
CTGCCAACTA ATCCGCACAT TCTAGACGCC TTTAAGAGTC TGGTTACGGA CCTAAGCGAA 
CTCGAGCAGC TTGATGCTGG CGGAGAGGAG GAGCAACTGG CCACCAAGCG CAAGCAAATC 
AAGCAGAAGT TCCGAGATCT TCTCGACCCG AACAACTTGC AAAAGTTCAT GTACTCCCTC 
CATATTGTGG AGAGTCTGGC CCTAACAAGT TCCAGACGTG GTGAATCCAA CGGCAATGTG 
GCCATGGGAA ATACACCGGA ACAGGTACGG GTGAAGAAAT CCTCGATGGG AAGACGGAGA 
AATAGCAACG AGCAGCCGCC ACCGCCGCCG CCAGAAGTCA AGATGAGCAA GGAGCAACTG 
TGCGAGCTGG AGGCCCCACT GACGCCCACA CCCTCGACTG GCTTGCAGGA TGTGGAAACG 
GAGGCCAGCA GTAGCAGCGG TGGCGACAAA GAAAACCAAC CGAAACAACA CAGCAAGCGT 
CAGAAGAAAG GCGAAACTTT TGAACAGGAA AAGGAGCGCC CAGTGGGTTG CTCCACGCCG 
CCGTCTCCAA CTCCTCCGCC ACCAGCACTT TCGGTAGTGG AAAGAGGTGA CAATAAGTGG 
AGTGAGGCGT TCGTCAAATG TGGTGGCCTT CGTCATCTGT ACGAAATCTT TAGTGAGGGC 
CAACTACAAC AAAGCGCACA TCCCAAGGAG TTGGCTTTAA ATGAATGGCG ACACGATTGC 
CTGGCCAGCC TGCTGCGCAT TCTCTGGCTT CTTGGATTCG AGGAACTGCA GTCGGCGGAT 
GCCCATGTTC TAATGTCACG TCCGCACCCG TTTATGCTAC AGTTGATGGA GGTACCACAG 
TGCCTGACCC GACTCTCATC GATCCTTAAC GACGAAGTTC ACCAGCAGCA TCAGGCTTCT 
TCGGCCAATC CACTGGTATT CCCCTATCAG TTCCAGCACT TGCGTACTGG CTTTTGGGGC 
AGGGCCCAGC TCATCCAGTT CGCTATGAAC ATTCTAGTGA GTTTTGTGCA CGCCTCAGCG 
GAAGCGAGAA GGCTTCTCTG GGCCCCAACT GGTACCGATC ACTGTCGATG GCTGCAGAAG 
TTCATCCTGG AGGATCCCGA ACCGGCGGTA CGGCGTGAGA TATGCGCTGG TCTTTATCGC 
ATTTGCTTAG GAAACGCACA TAGCTATCGC CTCTTACTAG CACCACTGCT GCACAAGCTC 
ATCGCCCTGT TGCCATTGGC CGAGCAGATG AGCTCTGGCA ACCAGCACAC GCAGTTTTTG 
CTCTCGGAAG AGGGCAAGGA TCCCTACGGA CCCGCCTGCC GTGACTACTT TTGGCTGCTG 
GCCAGATTGG TGGATACTCT CTCCCCGGAA ATGGTGGCCG AGGAGCACAT CGACATTGAA 
ATGCTGTGCG AAAGCATCTC GCAGAGCATT CTCACCCGAG AGTATTATGA ACTGCGCCAT 
GGTTACCAGG ACGATGGCTT GGTGGGTCTC CTAAATCTCA TGTCCAATTT GATCAAGTAC 
GACACCACAT TCAAGTACAC ACCCAAGGCG TTATCCTTTA TTGAGCAACT GATTGGCTTC 
CTGTTTGACA TGCCCTCGCC GGCAGATCGC CAGAAGCCCA AGTGCAAGTC GGCGTCGTCT 
CGAGCATCTG CCTACGATCT GCTGGTTGAA CTTTGCCGTG GATGCGCCAC AAACTATGCA 
TATCTGCATG GACGTCTGTT GGCACAGCAC AAATCCGGAC CCAAGCAGCC GTATCCCTGG 
GACTATTGGC CGCGCGATGA AGGCAGAGCT GAGTGCGGAT ATGTGGGGCT AACTAATTTG 
GGAGCCACTT GCTATATGGC CTCTTGCGTC CAGCACTTGT ACATGATGCC ACAGGCGCGA 
GCAGCTGTTT TGCGAGTTCC ACCCAACGCC GCCCGTAAGC ATGGGCCCAC ACTTCTGGAA 
CTACAACGGA TGTTTGCCTA CCTACTGGAG TCGGAACGGA AGTCTTACAA CCCGCGAAGC 
TTCTGCCGGG TGTACCAGAT GGATCACCAG CCGCTAAATA CGGGAGAGCA AAAGGATATG 
GCAGAGTTCT TTATCGATTT GGTGTCCAAA CTGGAAGATA TGACGCCTGA CTTGAAGCAC 
CTGGTGAAAA GGCTCTTCTG TGGATCCTTG AGCAATAATG TCGTCTCCCT GGATTGCGGT 
CACGTTTCCC GCACGGCCGA GGACTTTTAC ACGGTTCGCT GCCAGGTTGC AGATATGCGC 
AATCTGCAGG AATCCCTGGA CGAAGTCACC GTTAAGGATA CCCTCGAGGG CGACAACATG 
TACACCTGCT CGCAGTGCGG CAAAAAAGTA CGAGCCGAGA AGCGGGCTTG CTTCAAGAAG 
CTTCCCCAGA TCTTGTGCTT CAACACCATG CGCTACACCT TCAATATGGT CACCATGCTC 
AAGGAGAAGG TCAACACGCA CTTTTCCTTT CCGCTACGCC TGAACATGTG TCACTATGTG 
GAGAAGACCT TGATGCCGCA GCAGTACAAA GAGGAGCGAG AAAGGCGCCA AAAGGAGAAG 
GAAGGAGCAG ATGGTAGTGG CGATGGGAAT GACAACGAAA AGGCAGAGGC CACGCTAGAT 
GACGATATTG AAGAATGCTA CGAGTACGAA CTGGTGGGCG TCACTGTCCA CACGGGCACA 
GCGGATGGCG GCCACTACTA CAGCTTTATA AAGGAGCGGA CGAAGACTAG TTACCATACG 
CACGAGCGGT GGTTTCTTTT CAACGATGCC GAAGTGAAGC CTTTCGATCC CTCACAGATA 
GCGGCGGAGT GCTTTGGCGG CGAGATGACG AGCAAAACCT ACGACTCAGT GACGGAGAAA 
TATCTGGACT TTAGTTTCGA GAAGACCAAC TCTGCCTACA TGCTGTTCTA CGAGCGCCGA 
CTGCCAGAGC ATCTGCAGCG CCGGCATTCC GAGCTGCTGG TTACACCCAC GCCCAGTCCC 
ACAGTGGAAG AGAAGTCCGA GGCGGAGGAG CCCACTAAAA TGGAGACAAG CTCTAGCGAA 
ATCAAGGCAG ATGTGGATGT GGAAGTGGAA GTTGAGGAGA AGGACAAGGA GAAGCCTGCG 
CAGACTGACA CAGAATCTAA GGAAACGCCT GCAAAAGAGG AGATCGCGGA TGACAAATCG 
AAGCAGGATG AGCCTGAGGA AAAGAAAATC GAAAAGCAAT CCAGAGAGGG CGAAGAGAAA 
TCGGAAACGG ACGAAAAGCC CACGGAGATG ACGACCGTGA GCACTGAGGA GGAAAAGCAG 
CCAACAGCCA ATTGTGATAA TCACCAGCAA AACAACAATA GCAACAGCAA AGCCAGCAAC 
GATCAGCAAC CATCCACGTC GAAGGCTGCC CAGAAGCTAC AACTATTTCG TCCATTGTTG 
AACAAGGAGC TCGAGGATTG GATTTGGCAG GACAATCGAC AGTTCCTGCA GGATCGCAAC 
ATTTTCGAGC ACACATATTT CAACTTTATG TGGCAGATAT GTGGGCACAT ACCCCAATCA 
TTAATATCAG AGACGGACGT CACCTGCATG GCAGCCAAGT TGTCTGTGTC CTTTTTCATT 
GAGACTTTTA TTCATGCTAA GGAAAAGCCC ACTATGGTGC CCTGGGTGGA GCTGCTGACC 
AAGCAGTTCA ATGCCAGCCA AGAGGCCTGC GAGTGGTTTC TGTCGCACAT GTCGCAGGAG 
CCCTACTGGC CGGTCCAGGT GCTAATCCAG TGTCCCAACC AAATGGTGCG TCAAATGTTT 
CAGCGTTTGG TAATCCATGT CATCCAGCAA CTACGTGCCT CACATGCTCA TCTGTATCTG 
GAAGTGGAGA CAGACGAGGA TGACAAGGAG TTAATAGGCC AAGCCTCTTG CGTCACCCGT 
TTCATTGGTT CGCTCATCTC GCTCCTGGAG CACGGTGCCC GAGCGAACCT GCGGCACTTG 
TCCGAGTACT TTGGACTGTT GTGCGAGTTC TCACGGATGG GAGATGAGGA AGCCATGTAC 
TTGCTGCGCA TCGGTGTACT CAAGAGCCTG GTCGACTTCT ATCTGGGTCA CAAGCAAACA 
GACAGCATAG ACATAAGCTC TGACAACGAA GACAACTCCT CTGAAGAGGC GCTGTCCGTG 
GAGAAGATGC GTCCGGCCTC GCTGGACAAG ATGATTGCTC TGTGCGCCAG CCTAGTGGAA 
AGATCAAGAG GAGCCGACTT CCGGCTGCGA CTCTCGCCCA AAGACTTCTC TGCAATCGCC 
GGCGGCAAGG GTTTTCCATT TTTGTACCAA CAAATAAAGG ACGGCATTAA TCCGCACCAG 
ACAAAGCATT TAATACACGC GCTTTGTCGC TGGGATGAGC GCCTGGCCAC GCAGATCATT 
GGCATGCTCT TCGCGTCGGT AACCAAGCAC ACGGAGCTGT GCGCGCCATT CTTCAAGCTG 
CTAACTCTGC TCACCGAGAC GCAGGGCGGT CCAGTGGGCC TGCCTTGTTT CACCCAGTTG 
ATCCTGCCAC GAATGTGGGA CGCCGCCGAG TACTGTCCAC AGTCGGTGCT CGACTGGCTC 
TCGCTGCAGG CCACCAAGAA CAAGATCGCC CACGCCTGGA TACTTCAATC GGCCGAGAAG 
TGGCTCGAGC AATTCCTGCT GGCCCACGAT AACACTCGTG TTCGGAACGC TGCCGCCTTT 
CTGCTGGTGG CACTGGTACC CTCCCAGCCA TTCCGGGCCA ACTTCCGCGC CCACTCGCAA 
CACAAGCTGC TGGCCCTCAA TCCGCACAGT TATCGCGACA TAAATAGCGA TGCCCAGGCG 
GTGCTGCACC AGGTGATCAC GCTGCTCCTG CGATTGCTGC GTCCAGCGCG CGTCTATGCG 
GATATCGGAG CCCATGGCAC GACCAAGCTG ACCGCGTACT TTAATCTGCT CAGCTATTGT 
ATGGTTTCAA AGACTGAAAA ACTAATGGCA AGTTCGTACA TGCGCTCCCT GTGGGAGCTG 
TTCCATCCAC GCCTCTCGGA GCCCAGTGTT CCGGCCCATC ACAATAAGCA CGCACTATTA 
ACCTTCTGGC ACCACTCACT GGTGGACTGT CCGGAGAATG CTGCCCAGGT GGCCAACTGC 
CCGGAGATTA CGCGCAACAT TGCATTTAAC TATATACTGG CCGATCATGA TGACGCGGAG 
ATAGTCACCT ACAACCGTTC CATGCTGCCC GCCTATTATG GACTACTTCG TCTGTGCTGC 
GAGCAGAGCC GTGCACTCAC ACGCCAATTG TCCCAGCATC AGAATCTTCA GTGGGCATTT 
AAAAACATCA CACCGCATCC CACGCAGTAC GCGGCCGCCG TGGACGAGCT TTTCAAGTTG 
ATGGCTCTGT TCGCCACCCG TCATCCGGAT GCTAGTGAAC AGGAAAAGCT TGATGTGACC 
CAGTTTAGGA GGGCTGTGAT TGTGTCCTAC ACCAGTAGTC TGGATGCACG TGTCTCTTGG 
TCCACGCTGA TCAGTGCTCT TAAGATACTC GTGGACAATG AGGAGGACCG CACGATGGTG 
ATCTTCAACG GCGGCATTGA GATGTGTTTC GAGGCGCTGC ACACGCTACA TTCCATGCAC 
CACGAGGCCA CCGCCTGCCA CGTGGCCGGA GACCTATTCG ATCTCTTGGG CGAGATGCTA 
CTCCTCCTAG CCACTCTTCG AACCCGCACA GACAGTCCTG CTCAAAAGAA GCAGCAGCAA 
CAACAGCAGC AGCTGGAGCA ACAGTTGCAG TTGCAGCAGC AACAGATGCT GCAGAAACAA 
CAGCAGCAGT CGCAAAGCCA AACGCAAACA CCGCAGTCGC CGCAGCAAAA GGAAAAGCAG 
CTGCAGCAAC AAATGCAGCA GCATCTGCAG CTGCAACAGT TGCAGCAAAT GCAGTTCCAG 
CAGCAACACT TCCTGCGCCA GCAACATCAA CACTCGGCGC TGGCAAAGGC ACTGCCGGAT 
GCAGTGAAGC GACTGGCTAC GCTACTCAAC ACCTTCAATT CGCCAGAGAT CAGTCGCATG 
GCCTTGGAGG TGCTCAAGGA GCTGGTGCGC AATCCCAGCC TGGAAACCAT TAGTATTCTG 
GCGCCCATTC TTATCAACTG TCATCTGTCC GTGGCGAATG CTCCCAATGC GATCGGTCCT 
CTGGGACCGT ACTTTCCGCG TCGCGGTGCG AAGCATACGC CTTGGCCATT GGGAGCCAAG 
AACTCACCCC GTCCACCGCG GCCCATGGTA CAGATGTGCA TCGCTCTGGC TGAGCTGACA 
CCACGCGGTC TCGATGCCGA TTACGACGTT CAGGTGGAGT CCTTTTACCG ACCCTACCAT 
GACTTTATCG ATGTCATGAT GCGCATGTGC GTCAACACGG GCACTCTGAA CGACACGCTA 
GTCAAGCTGC AATGCTTGGT GGCCATCGAG TCCACGCCGC TGCACTTCAC CTATTTCCCC 
AAATTCTGGG TGGGCATACA CAACAATGCT CTGACGCACA AATACGTTGA GCTGCTGGTG 
AAAAATCAAC TGTTGGTAGA GTACCTTCAC AATGTCCTTA GGGACGAGCG AAGCATGTTG 
AAGGATGCCT GCGTTCGTGA ATTTCTGGAG CTCTATTATC ACAAGGTTGC CGCCCAGCTG 
CCAGTGGCCC GTATGATCTA CACAATCAAC TATGGTATGC ACTCCAAGGA CGACATTGAT 
GAGCTGTGTG GCGATCTGTT CGCCATCCGA ATCATTGCCC AGGCCACTGG AGTGCCGGCC 
TCAGTGCGCA AGGAGTTGCG AGGATCGCTG AGGGCGCTTC AGAACAAGAG CGAGCGGTTT 
AGGAAGGAAT CGGAGAGGGA TCCATTTCCG AACAAGAAGC AGAAACGTGA CTCGCAAAAG 
ATCAAGGAAA AGGAGCACCC TCAGCCGGAG AGCGAGAAAG AGACCTCAAC GGAGAACGAC 
AAACCATCAG ATGTTTCCAT GGAATCCAGC GGTAACGCGG AGCAAGCAAC GGACAGCACA 
AAACCACCAA CTCCTGCAGG CAGCGACGAT GAGCAGATGG ACAAAACTTC AGTTCCCTCG 
TCCGATGACG AGACAGAGTT GGAGGACGAG CTGCAGCCGA CGCCCAAGCG CAACAAGAAG 
GCTTCGAATA AGAAGACCGC CCAGGATCGT GTCAACGAGG AGCGCGAGAA GCGACTTATC 
ACGCTGATCA CCATGGAATC ATATATCCAG AGCATTTTTG CCATCCTTAA GAGAGATGCC 
AGTGTCCCCA GTTCGGGAGC GACTAAGGAG CCCAGTGAGG AACCGTGTGG CGAGGCATCC 
ACTTCGGCGG CTGCCGCTGT AAAGTTATCT CGTCCCAAAG GAGCCTGTAC TCCGCCGGAG 
CCAATTACCG ACGTAAACGA TACGCGTTGC AACATAGATA CTGAGACAGA GGCGGAGGCG 
GATGAGCAAT CTCCAAAGAC GAGCCAGACA AATGGTAGCC AACAGAACGA ATCCCCGCCA 
GCGGCGACCA GTGCAGACAC TGCACCCGCG AATCCTTCAC CAGCTCCCGC GGCCGCCGTT 
GCGTCCACAT CCCAGGCGGC AAGTCCAACA CAGATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

puf ( NM_206564.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996287.1 Drosophila melanogaster puffyeye (puf), transcript variant E, mRNA.

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CloneID	OFa05979
Clone ID Related Accession (Same CDS sequence)	NM_206564.2
Accession Version	NM_206564.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	puffyeye, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_206564.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCGAGG TGTGCGCCGA CTTCCAGAAT CTCCTGGAGC TCTATGAGGT GCGCGTGGCC 
AGCTCGGATC TGAAGTTCCA GCTGCTGCTG AAGAGCGAGA TCGAGACCAC CTTCAACTAC 
ATCCAGTCGT GGCCGCAGCG TCAGTGCATG TGCCTGTACC GGGACACCAA GAACTATGAC 
CGTTTCAACC TGGTAGTGCA GTCGCTCATC TGTCTGACGG TGCAGCATCT GAAGCACATC 
GACCACCTCA TCGACAACTA CAAGCGGCTA ACGGCCAGTG CTGCCCAGGT GGTGGCCCAG 
GCCCAGCAGC AGCGTGAACA GCAGCGGGAC GAAGCGTCAG CCCAGGCAGA GGCAAAGGAA 
TCATCCGCGC CAGCGGAAGA ACCTAAGAAG GAGGAGCCAT CTGGGTCCGC CGGTGAGGAG 
GCTCAGGGCT CCGGCGACGG GCCAGCCAAA AAGCCGCCAG TTGGCCCCTG CACTCCCCCT 
CCCCCGCAAA CGGCCAATCC TCAGCACAAA TCCCACCTGC AGTACACAGA GGAGCCCTGG 
ATCCTGCCCG AGGTGGAGAA ACTGTTGGTG CTCGTTTCAA AGGTGTTCCT GCTCAACTTC 
CCGCTGTACA TCGCCCACAA ACATGGCATG CACTCTCGGC TGGACGACCT GCAGGCTGAG 
GAGGCCCACC ACCTGGCCCT CATCTGCGAT CTTCACGACA ACGACCTGCC CATCTATCTG 
CTGCGGAATG TGAGCCTGTT CTGCAACTCG GGCGGCTTTG GCGCCATGTC GCTGTGCTTC 
GAACATCCCG ATCTGCCAGT GTCCACCGCT CACTCGATGA CCGCTGCCGT CTCAAATGTG 
AAACTTTGGC TGAACTATCA CTGTAATACT CAGCTCTTCG TGCCGCTGCG AAGTCGGATA 
CTGCAGTACA TGTGCAAGCT GTCGGATCAG AGCCTCCGAT CTGCCGCAAC CCGCGCGATG 
GCCGATTTTG TGTGGTCCTC TATGCGAGAC CCACTGGATG TTGCTGTCAA CTTCGACACC 
GAAGGATTGG CCCTGGCCTT TAAGTACTTC ACATCGACCA CGCTAACCAT GCGACTGGCA 
GGCATGGCCC AGATCAACGC ACACATTAAT CTATTTAACG AGATCTGCAC CACCGAAACG 
GTCAACGAAG TGGAGCTGTT CGGGCAGCGC ATCGCGAACT GGCTGACTGA AAATCATATT 
GTACAGCACC TGTTTGGCCC GAATCTGCAC GTAGAAATCG TTAAACAGGC ACACGTGCTG 
CTCAACTTTT TGGCGGTAGA GAACCAGATC TCTGAAGAGG ACATCAAGCT GATCTGGCAA 
GCCACCCAGC TGAAGCACTG CTCAAAGACG ATCTTCGACA TCCTGCCTTC GCTGGTGAAG 
AATCTGACCC CGCGCCCGGC AATGCACCTT TACTCCCTCC TTTGCCGCAT GGATCCCAAG 
GAGCACACTG AGCAGAGCAT ATACATAGCC TCAGCGCTAA CAAAACAGCT GTGGACTCGT 
GATACCTCAC GCAGTCAGAT GAACCTGATG CAAGACCACC TGCTGGGGTC CAACGTCACC 
GCTTCCAGCT CTGACAGCGG CAGCATTGAG GGCAGCAACA CAGAGGACGA CCACGTGGGC 
GCCGACGACA GTAGCATTGC CAGCGGTGGC GGCGGCGGAG GTGTTGGTAA CAAGAGTCCA 
ATCGATGGAG TTACTCCATG CAAGCAGGCC CGGCACAGGC GGCACATCTG TGACCCCACC 
ACCGAGAAGG GAAAGCAAAT AAGTCCCGAG GATATGGCCA AAGTAGACTT GGTGAACAAA 
CGGATTGTGA ACATTATCGA CAACACAAGC AGTGAAGAGG AGCTGCAATC GCGGGCCGCA 
CTGGAGCTGC AACTGCATCG CAGCCGCAAA AAGACGAGCA ACAAGCGACG GCGACAAAAA 
ACCAACAAAC AGATCATTCT GCCCCATGAG CTGGTCGAGA TTTGGGACGG AGTTGAAGAT 
GTGCCCAGCA GTGACGAGGC TGATGGCGAA GCCGATGGTG ATGGCGAGGG CGAACTTTTA 
GCGGACAGCG ATGAGTGCAG CGATGGTGCT ACGAGTCAGC TGGTACCCGA CGCCGTGCTA 
AAGCATCTGC AGGGAGAGGG ACCTTTTATC AGGGCCATCG AGACCAATAT CAATGAGCTG 
CTCAGTGGTG CCGAGAACGA TGGCAGTTAC TCATCCCCGA TGAGTAACAA ATCTGAAAAG 
AACCTGGCTG ACTTCGATGA CGAGGACGTG TCGCCTTGCG AGGAGGAGCT GGCCCAGCTG 
GTCAGCTCCC GCGCCAATTG TTCGGACGTT CCACCAGCCT TTGCAGCCGC TGCCGCCGCC 
ATGATGGTGG CCCAGTCGGC GATGGCCCTT CAGAAGTCTG GAGAGTCGAA TGTGGCTGCT 
GCTGCCGCTG CTGTGGCCGC TGCTGCAGCG GCTACCGGAA CTGCACAGCA AACGCTGGTG 
GCAATGAACC GGCAGGCCAG CGTTGCTGCA GCTGCCGCCG TGGTTGCTGC GGCCAAGGCA 
AAATCGGACT CAGATGTCGA TCTGATGGAT GTGGTTTCCG GCGGAAAGCA GCACCATTCA 
CCAAAGGCGA GCCAGGGCAG CTCTACGTCC GGGAGTACAC CGGTGCAGCC CTCGTTCAAG 
CTAAATGATG TCTGCCAGCC CGGAAACACA CTGCTTTGGG ATCTGCTTCA AGACGATAAA 
ATTGGCCAAC TAGGCGAGTC TCTGGCTCTA GAGGCGGAGA AAGCTCTGGC CACTCTCCTG 
TGCTTCAGCA TGGACCGCCA ACTGCGAACG AAATTCATCG AGGGTTGTCT GTATAACGTC 
GCCAACAACC GGAGTGTTAT TGTCAGTCTG CGACTGCTGC CCAAACTGTT CGCCTCGTTC 
CAACAGTTTC GACCCTCGGA CACGCACTCT ATGACGATGT GGGCAGAGCG AAATCACCGG 
ATGATGCAGT GCTTTTTCAA TAATATACGT CACTATGCGC GTCGCCATGC CGAGGTACTG 
ATCACCCAAA ACGGAGAGCA GCAGCAGCAG CAGCTGGGCG GACAACTCTA CTCGCACAAG 
ACACAAGTTT CTGTAAGACT GCAGTTCCTA TCATCGATAT TCTCGACGGT GGGATCCCCG 
AAAAGCTTCC GCCTAACGCT GGAGCAGCTT GACGCGCTAT GGGAGTGGCT GGCCCACGAT 
CCGGAGTGTG CCGACTGCTA CTTTTCGTGG CTGCAGGCCC AGGCCAAAGG CGGTGATCAG 
CATGCTCTGG GCATTGAAGC ACTTCAGCAT TTGTATTTGA AGAAACTCCC AGAGCTGCGA 
CCCGAGGAGT TCTCTATGGT TGCGTTGGGA CTATTTCAAC AGCTCTGTAG CTTCGCCCGC 
ATCGCCATGG CCGAGTACGA CAATCATAGC GACCAGATCT CGGCGAGCGC CAGTGCCGTT 
GGCATGTACC ATCTTTGGAA GATAGCGTTG CGTGCCCAGA GCAACGATGT CTCCTTGGCG 
GCCATACAGT ACATCAACAT GTACTACATG GGTCAACAAT TGCGCCTGGA GAAGGAGTTC 
GTTTCACAGT GTATGGAAAA CCTAGTTCAA GCGGCCACGG CTTTGGAAAG CATCGATGAT 
GAGAATGCTT TGATGCGTGT CCAGCGTGGC CTGCTCTTGC TGAACACCCA TTTGGATACG 
TTCCGGCGTC GATATGCGTT TCATCTGCGC CGCTGGGCCA TCGAAGGCAA AGGGATTGGA 
TCGCACAGTA ACCTTAAAAA TGAGGGAGCG GGTCCACCAC TACGAATAGT GCTGCAACAG 
GCAGGACTTT CGGAAAAAAG CCTTTTGCAG ATGCACGCGT GTGACTTAAT CGCCGACCTC 
AAGGCTGAGG TGTCCAAGTG GTGGGAGAGC CTTCAAACTG GACTAGCTGC TCCTGTGCTC 
GGTCTGCTGC TCAGCGATGG ACCGCTACGC ATTATCACAC AGGGACAGGA ATTGACCTCG 
GATTACGATG AACGGAGTCT CGGAGATGCT GGTTTCAAGG ATAACCAGAT CGTCTACGTA 
TCGTTAGGTG GACGTGGAGC CAGGCGAAAG GAATCCAATT TGGAGCATCC GTCAATGCTG 
CCGCCCCCAC CAAAGGAGTG TTTGCCCACG GTGCTCCTGC TGCAGCCAAA GTACTTCGAG 
AAGCTATTTT GCCTGATGCA GACCCTGGGC GATATGCAAC CGCAAGCGTC GACTGTAAAT 
CCCCAGCACC ACACAAAGGC GCAATTGCTC TCGCGACGCG TATGGGATAT CCTCGCCATG 
CTGCCAACTA ATCCGCACAT TCTAGACGCC TTTAAGAGTC TGGTTACGGA CCTAAGCGAA 
CTCGAGCAGC TTGATGCTGG CGGAGAGGAG GAGCAACTGG CCACCAAGCG CAAGCAAATC 
AAGCAGAAGT TCCGAGATCT TCTCGACCCG AACAACTTGC AAAAGTTCAT GTACTCCCTC 
CATATTGTGG AGAGTCTGGC CCTAACAAGT TCCAGACGTG GTGAATCCAA CGGCAATGTG 
GCCATGGGAA ATACACCGGA ACAGGTACGG GTGAAGAAAT CCTCGATGGG AAGACGGAGA 
AATAGCAACG AGCAGCCGCC ACCGCCGCCG CCAGAAGTCA AGATGAGCAA GGAGCAACTG 
TGCGAGCTGG AGGCCCCACT GACGCCCACA CCCTCGACTG GCTTGCAGGA TGTGGAAACG 
GAGGCCAGCA GTAGCAGCGG TGGCGACAAA GAAAACCAAC CGAAACAACA CAGCAAGCGT 
CAGAAGAAAG GCGAAACTTT TGAACAGGAA AAGGAGCGCC CAGTGGGTTG CTCCACGCCG 
CCGTCTCCAA CTCCTCCGCC ACCAGCACTT TCGGTAGTGG AAAGAGGTGA CAATAAGTGG 
AGTGAGGCGT TCGTCAAATG TGGTGGCCTT CGTCATCTGT ACGAAATCTT TAGTGAGGGC 
CAACTACAAC AAAGCGCACA TCCCAAGGAG TTGGCTTTAA ATGAATGGCG ACACGATTGC 
CTGGCCAGCC TGCTGCGCAT TCTCTGGCTT CTTGGATTCG AGGAACTGCA GTCGGCGGAT 
GCCCATGTTC TAATGTCACG TCCGCACCCG TTTATGCTAC AGTTGATGGA GGTACCACAG 
TGCCTGACCC GACTCTCATC GATCCTTAAC GACGAAGTTC ACCAGCAGCA TCAGGCTTCT 
TCGGCCAATC CACTGGTATT CCCCTATCAG TTCCAGCACT TGCGTACTGG CTTTTGGGGC 
AGGGCCCAGC TCATCCAGTT CGCTATGAAC ATTCTAGTGA GTTTTGTGCA CGCCTCAGCG 
GAAGCGAGAA GGCTTCTCTG GGCCCCAACT GGTACCGATC ACTGTCGATG GCTGCAGAAG 
TTCATCCTGG AGGATCCCGA ACCGGCGGTA CGGCGTGAGA TATGCGCTGG TCTTTATCGC 
ATTTGCTTAG GAAACGCACA TAGCTATCGC CTCTTACTAG CACCACTGCT GCACAAGCTC 
ATCGCCCTGT TGCCATTGGC CGAGCAGATG AGCTCTGGCA ACCAGCACAC GCAGTTTTTG 
CTCTCGGAAG AGGGCAAGGA TCCCTACGGA CCCGCCTGCC GTGACTACTT TTGGCTGCTG 
GCCAGATTGG TGGATACTCT CTCCCCGGAA ATGGTGGCCG AGGAGCACAT CGACATTGAA 
ATGCTGTGCG AAAGCATCTC GCAGAGCATT CTCACCCGAG AGTATTATGA ACTGCGCCAT 
GGTTACCAGG ACGATGGCTT GGTGGGTCTC CTAAATCTCA TGTCCAATTT GATCAAGTAC 
GACACCACAT TCAAGTACAC ACCCAAGGCG TTATCCTTTA TTGAGCAACT GATTGGCTTC 
CTGTTTGACA TGCCCTCGCC GGCAGATCGC CAGAAGCCCA AGTGCAAGTC GGCGTCGTCT 
CGAGCATCTG CCTACGATCT GCTGGTTGAA CTTTGCCGTG GATGCGCCAC AAACTATGCA 
TATCTGCATG GACGTCTGTT GGCACAGCAC AAATCCGGAC CCAAGCAGCC GTATCCCTGG 
GACTATTGGC CGCGCGATGA AGGCAGAGCT GAGTGCGGAT ATGTGGGGCT AACTAATTTG 
GGAGCCACTT GCTATATGGC CTCTTGCGTC CAGCACTTGT ACATGATGCC ACAGGCGCGA 
GCAGCTGTTT TGCGAGTTCC ACCCAACGCC GCCCGTAAGC ATGGGCCCAC ACTTCTGGAA 
CTACAACGGA TGTTTGCCTA CCTACTGGAG TCGGAACGGA AGTCTTACAA CCCGCGAAGC 
TTCTGCCGGG TGTACCAGAT GGATCACCAG CCGCTAAATA CGGGAGAGCA AAAGGATATG 
GCAGAGTTCT TTATCGATTT GGTGTCCAAA CTGGAAGATA TGACGCCTGA CTTGAAGCAC 
CTGGTGAAAA GGCTCTTCTG TGGATCCTTG AGCAATAATG TCGTCTCCCT GGATTGCGGT 
CACGTTTCCC GCACGGCCGA GGACTTTTAC ACGGTTCGCT GCCAGGTTGC AGATATGCGC 
AATCTGCAGG AATCCCTGGA CGAAGTCACC GTTAAGGATA CCCTCGAGGG CGACAACATG 
TACACCTGCT CGCAGTGCGG CAAAAAAGTA CGAGCCGAGA AGCGGGCTTG CTTCAAGAAG 
CTTCCCCAGA TCTTGTGCTT CAACACCATG CGCTACACCT TCAATATGGT CACCATGCTC 
AAGGAGAAGG TCAACACGCA CTTTTCCTTT CCGCTACGCC TGAACATGTG TCACTATGTG 
GAGAAGACCT TGATGCCGCA GCAGTACAAA GAGGAGCGAG AAAGGCGCCA AAAGGAGAAG 
GAAGGAGCAG ATGGTAGTGG CGATGGGAAT GACAACGAAA AGGCAGAGGC CACGCTAGAT 
GACGATATTG AAGAATGCTA CGAGTACGAA CTGGTGGGCG TCACTGTCCA CACGGGCACA 
GCGGATGGCG GCCACTACTA CAGCTTTATA AAGGAGCGGA CGAAGACTAG TTACCATACG 
CACGAGCGGT GGTTTCTTTT CAACGATGCC GAAGTGAAGC CTTTCGATCC CTCACAGATA 
GCGGCGGAGT GCTTTGGCGG CGAGATGACG AGCAAAACCT ACGACTCAGT GACGGAGAAA 
TATCTGGACT TTAGTTTCGA GAAGACCAAC TCTGCCTACA TGCTGTTCTA CGAGCGCCGA 
CTGCCAGAGC ATCTGCAGCG CCGGCATTCC GAGCTGCTGG TTACACCCAC GCCCAGTCCC 
ACAGTGGAAG AGAAGTCCGA GGCGGAGGAG CCCACTAAAA TGGAGACAAG CTCTAGCGAA 
ATCAAGGCAG ATGTGGATGT GGAAGTGGAA GTTGAGGAGA AGGACAAGGA GAAGCCTGCG 
CAGACTGACA CAGAATCTAA GGAAACGCCT GCAAAAGAGG AGATCGCGGA TGACAAATCG 
AAGCAGGATG AGCCTGAGGA AAAGAAAATC GAAAAGCAAT CCAGAGAGGG CGAAGAGAAA 
TCGGAAACGG ACGAAAAGCC CACGGAGATG ACGACCGTGA GCACTGAGGA GGAAAAGCAG 
CCAACAGCCA ATTGTGATAA TCACCAGCAA AACAACAATA GCAACAGCAA AGCCAGCAAC 
GATCAGCAAC CATCCACGTC GAAGGCTGCC CAGAAGCTAC AACTATTTCG TCCATTGTTG 
AACAAGGAGC TCGAGGATTG GATTTGGCAG GACAATCGAC AGTTCCTGCA GGATCGCAAC 
ATTTTCGAGC ACACATATTT CAACTTTATG TGGCAGATAT GTGGGCACAT ACCCCAATCA 
TTAATATCAG AGACGGACGT CACCTGCATG GCAGCCAAGT TGTCTGTGTC CTTTTTCATT 
GAGACTTTTA TTCATGCTAA GGAAAAGCCC ACTATGGTGC CCTGGGTGGA GCTGCTGACC 
AAGCAGTTCA ATGCCAGCCA AGAGGCCTGC GAGTGGTTTC TGTCGCACAT GTCGCAGGAG 
CCCTACTGGC CGGTCCAGGT GCTAATCCAG TGTCCCAACC AAATGGTGCG TCAAATGTTT 
CAGCGTTTGG TAATCCATGT CATCCAGCAA CTACGTGCCT CACATGCTCA TCTGTATCTG 
GAAGTGGAGA CAGACGAGGA TGACAAGGAG TTAATAGGCC AAGCCTCTTG CGTCACCCGT 
TTCATTGGTT CGCTCATCTC GCTCCTGGAG CACGGTGCCC GAGCGAACCT GCGGCACTTG 
TCCGAGTACT TTGGACTGTT GTGCGAGTTC TCACGGATGG GAGATGAGGA AGCCATGTAC 
TTGCTGCGCA TCGGTGTACT CAAGAGCCTG GTCGACTTCT ATCTGGGTCA CAAGCAAACA 
GACAGCATAG ACATAAGCTC TGACAACGAA GACAACTCCT CTGAAGAGGC GCTGTCCGTG 
GAGAAGATGC GTCCGGCCTC GCTGGACAAG ATGATTGCTC TGTGCGCCAG CCTAGTGGAA 
AGATCAAGAG GAGCCGACTT CCGGCTGCGA CTCTCGCCCA AAGACTTCTC TGCAATCGCC 
GGCGGCAAGG TTATACTCAA AAAGCTGGAA AGCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996287.1
CDS	285..8783
Translation	MCEVCADFQNLLELYEVRVASSDLKFQLLLKSEIETTFNYIQSWPQRQCMCLYRDTKNYDRFNLVVQSLICLTVQHLKHIDHLIDNYKRLTASAAQVVAQAQQQREQQRDEASAQAEAKESSAPAEEPKKEEPSGSAGEEAQGSGDGPAKKPPVGPCTPPPPQTANPQHKSHLQYTEEPWILPEVEKLLVLVSKVFLLNFPLYIAHKHGMHSRLDDLQAEEAHHLALICDLHDNDLPIYLLRNVSLFCNSGGFGAMSLCFEHPDLPVSTAHSMTAAVSNVKLWLNYHCNTQLFVPLRSRILQYMCKLSDQSLRSAATRAMADFVWSSMRDPLDVAVNFDTEGLALAFKYFTSTTLTMRLAGMAQINAHINLFNEICTTETVNEVELFGQRIANWLTENHIVQHLFGPNLHVEIVKQAHVLLNFLAVENQISEEDIKLIWQATQLKHCSKTIFDILPSLVKNLTPRPAMHLYSLLCRMDPKEHTEQSIYIASALTKQLWTRDTSRSQMNLMQDHLLGSNVTASSSDSGSIEGSNTEDDHVGADDSSIASGGGGGGVGNKSPIDGVTPCKQARHRRHICDPTTEKGKQISPEDMAKVDLVNKRIVNIIDNTSSEEELQSRAALELQLHRSRKKTSNKRRRQKTNKQIILPHELVEIWDGVEDVPSSDEADGEADGDGEGELLADSDECSDGATSQLVPDAVLKHLQGEGPFIRAIETNINELLSGAENDGSYSSPMSNKSEKNLADFDDEDVSPCEEELAQLVSSRANCSDVPPAFAAAAAAMMVAQSAMALQKSGESNVAAAAAAVAAAAAATGTAQQTLVAMNRQASVAAAAAVVAAAKAKSDSDVDLMDVVSGGKQHHSPKASQGSSTSGSTPVQPSFKLNDVCQPGNTLLWDLLQDDKIGQLGESLALEAEKALATLLCFSMDRQLRTKFIEGCLYNVANNRSVIVSLRLLPKLFASFQQFRPSDTHSMTMWAERNHRMMQCFFNNIRHYARRHAEVLITQNGEQQQQQLGGQLYSHKTQVSVRLQFLSSIFSTVGSPKSFRLTLEQLDALWEWLAHDPECADCYFSWLQAQAKGGDQHALGIEALQHLYLKKLPELRPEEFSMVALGLFQQLCSFARIAMAEYDNHSDQISASASAVGMYHLWKIALRAQSNDVSLAAIQYINMYYMGQQLRLEKEFVSQCMENLVQAATALESIDDENALMRVQRGLLLLNTHLDTFRRRYAFHLRRWAIEGKGIGSHSNLKNEGAGPPLRIVLQQAGLSEKSLLQMHACDLIADLKAEVSKWWESLQTGLAAPVLGLLLSDGPLRIITQGQELTSDYDERSLGDAGFKDNQIVYVSLGGRGARRKESNLEHPSMLPPPPKECLPTVLLLQPKYFEKLFCLMQTLGDMQPQASTVNPQHHTKAQLLSRRVWDILAMLPTNPHILDAFKSLVTDLSELEQLDAGGEEEQLATKRKQIKQKFRDLLDPNNLQKFMYSLHIVESLALTSSRRGESNGNVAMGNTPEQVRVKKSSMGRRRNSNEQPPPPPPEVKMSKEQLCELEAPLTPTPSTGLQDVETEASSSSGGDKENQPKQHSKRQKKGETFEQEKERPVGCSTPPSPTPPPPALSVVERGDNKWSEAFVKCGGLRHLYEIFSEGQLQQSAHPKELALNEWRHDCLASLLRILWLLGFEELQSADAHVLMSRPHPFMLQLMEVPQCLTRLSSILNDEVHQQHQASSANPLVFPYQFQHLRTGFWGRAQLIQFAMNILVSFVHASAEARRLLWAPTGTDHCRWLQKFILEDPEPAVRREICAGLYRICLGNAHSYRLLLAPLLHKLIALLPLAEQMSSGNQHTQFLLSEEGKDPYGPACRDYFWLLARLVDTLSPEMVAEEHIDIEMLCESISQSILTREYYELRHGYQDDGLVGLLNLMSNLIKYDTTFKYTPKALSFIEQLIGFLFDMPSPADRQKPKCKSASSRASAYDLLVELCRGCATNYAYLHGRLLAQHKSGPKQPYPWDYWPRDEGRAECGYVGLTNLGATCYMASCVQHLYMMPQARAAVLRVPPNAARKHGPTLLELQRMFAYLLESERKSYNPRSFCRVYQMDHQPLNTGEQKDMAEFFIDLVSKLEDMTPDLKHLVKRLFCGSLSNNVVSLDCGHVSRTAEDFYTVRCQVADMRNLQESLDEVTVKDTLEGDNMYTCSQCGKKVRAEKRACFKKLPQILCFNTMRYTFNMVTMLKEKVNTHFSFPLRLNMCHYVEKTLMPQQYKEERERRQKEKEGADGSGDGNDNEKAEATLDDDIEECYEYELVGVTVHTGTADGGHYYSFIKERTKTSYHTHERWFLFNDAEVKPFDPSQIAAECFGGEMTSKTYDSVTEKYLDFSFEKTNSAYMLFYERRLPEHLQRRHSELLVTPTPSPTVEEKSEAEEPTKMETSSSEIKADVDVEVEVEEKDKEKPAQTDTESKETPAKEEIADDKSKQDEPEEKKIEKQSREGEEKSETDEKPTEMTTVSTEEEKQPTANCDNHQQNNNSNSKASNDQQPSTSKAAQKLQLFRPLLNKELEDWIWQDNRQFLQDRNIFEHTYFNFMWQICGHIPQSLISETDVTCMAAKLSVSFFIETFIHAKEKPTMVPWVELLTKQFNASQEACEWFLSHMSQEPYWPVQVLIQCPNQMVRQMFQRLVIHVIQQLRASHAHLYLEVETDEDDKELIGQASCVTRFIGSLISLLEHGARANLRHLSEYFGLLCEFSRMGDEEAMYLLRIGVLKSLVDFYLGHKQTDSIDISSDNEDNSSEEALSVEKMRPASLDKMIALCASLVERSRGADFRLRLSPKDFSAIAGGKVILKKLESF

Target ORF information:

RefSeq Version	NM_206564.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster puffyeye (puf), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206564.2

ORF Insert Sequence:

ATGTGCGAGG TGTGCGCCGA CTTCCAGAAT CTCCTGGAGC TCTATGAGGT GCGCGTGGCC 
AGCTCGGATC TGAAGTTCCA GCTGCTGCTG AAGAGCGAGA TCGAGACCAC CTTCAACTAC 
ATCCAGTCGT GGCCGCAGCG TCAGTGCATG TGCCTGTACC GGGACACCAA GAACTATGAC 
CGTTTCAACC TGGTAGTGCA GTCGCTCATC TGTCTGACGG TGCAGCATCT GAAGCACATC 
GACCACCTCA TCGACAACTA CAAGCGGCTA ACGGCCAGTG CTGCCCAGGT GGTGGCCCAG 
GCCCAGCAGC AGCGTGAACA GCAGCGGGAC GAAGCGTCAG CCCAGGCAGA GGCAAAGGAA 
TCATCCGCGC CAGCGGAAGA ACCTAAGAAG GAGGAGCCAT CTGGGTCCGC CGGTGAGGAG 
GCTCAGGGCT CCGGCGACGG GCCAGCCAAA AAGCCGCCAG TTGGCCCCTG CACTCCCCCT 
CCCCCGCAAA CGGCCAATCC TCAGCACAAA TCCCACCTGC AGTACACAGA GGAGCCCTGG 
ATCCTGCCCG AGGTGGAGAA ACTGTTGGTG CTCGTTTCAA AGGTGTTCCT GCTCAACTTC 
CCGCTGTACA TCGCCCACAA ACATGGCATG CACTCTCGGC TGGACGACCT GCAGGCTGAG 
GAGGCCCACC ACCTGGCCCT CATCTGCGAT CTTCACGACA ACGACCTGCC CATCTATCTG 
CTGCGGAATG TGAGCCTGTT CTGCAACTCG GGCGGCTTTG GCGCCATGTC GCTGTGCTTC 
GAACATCCCG ATCTGCCAGT GTCCACCGCT CACTCGATGA CCGCTGCCGT CTCAAATGTG 
AAACTTTGGC TGAACTATCA CTGTAATACT CAGCTCTTCG TGCCGCTGCG AAGTCGGATA 
CTGCAGTACA TGTGCAAGCT GTCGGATCAG AGCCTCCGAT CTGCCGCAAC CCGCGCGATG 
GCCGATTTTG TGTGGTCCTC TATGCGAGAC CCACTGGATG TTGCTGTCAA CTTCGACACC 
GAAGGATTGG CCCTGGCCTT TAAGTACTTC ACATCGACCA CGCTAACCAT GCGACTGGCA 
GGCATGGCCC AGATCAACGC ACACATTAAT CTATTTAACG AGATCTGCAC CACCGAAACG 
GTCAACGAAG TGGAGCTGTT CGGGCAGCGC ATCGCGAACT GGCTGACTGA AAATCATATT 
GTACAGCACC TGTTTGGCCC GAATCTGCAC GTAGAAATCG TTAAACAGGC ACACGTGCTG 
CTCAACTTTT TGGCGGTAGA GAACCAGATC TCTGAAGAGG ACATCAAGCT GATCTGGCAA 
GCCACCCAGC TGAAGCACTG CTCAAAGACG ATCTTCGACA TCCTGCCTTC GCTGGTGAAG 
AATCTGACCC CGCGCCCGGC AATGCACCTT TACTCCCTCC TTTGCCGCAT GGATCCCAAG 
GAGCACACTG AGCAGAGCAT ATACATAGCC TCAGCGCTAA CAAAACAGCT GTGGACTCGT 
GATACCTCAC GCAGTCAGAT GAACCTGATG CAAGACCACC TGCTGGGGTC CAACGTCACC 
GCTTCCAGCT CTGACAGCGG CAGCATTGAG GGCAGCAACA CAGAGGACGA CCACGTGGGC 
GCCGACGACA GTAGCATTGC CAGCGGTGGC GGCGGCGGAG GTGTTGGTAA CAAGAGTCCA 
ATCGATGGAG TTACTCCATG CAAGCAGGCC CGGCACAGGC GGCACATCTG TGACCCCACC 
ACCGAGAAGG GAAAGCAAAT AAGTCCCGAG GATATGGCCA AAGTAGACTT GGTGAACAAA 
CGGATTGTGA ACATTATCGA CAACACAAGC AGTGAAGAGG AGCTGCAATC GCGGGCCGCA 
CTGGAGCTGC AACTGCATCG CAGCCGCAAA AAGACGAGCA ACAAGCGACG GCGACAAAAA 
ACCAACAAAC AGATCATTCT GCCCCATGAG CTGGTCGAGA TTTGGGACGG AGTTGAAGAT 
GTGCCCAGCA GTGACGAGGC TGATGGCGAA GCCGATGGTG ATGGCGAGGG CGAACTTTTA 
GCGGACAGCG ATGAGTGCAG CGATGGTGCT ACGAGTCAGC TGGTACCCGA CGCCGTGCTA 
AAGCATCTGC AGGGAGAGGG ACCTTTTATC AGGGCCATCG AGACCAATAT CAATGAGCTG 
CTCAGTGGTG CCGAGAACGA TGGCAGTTAC TCATCCCCGA TGAGTAACAA ATCTGAAAAG 
AACCTGGCTG ACTTCGATGA CGAGGACGTG TCGCCTTGCG AGGAGGAGCT GGCCCAGCTG 
GTCAGCTCCC GCGCCAATTG TTCGGACGTT CCACCAGCCT TTGCAGCCGC TGCCGCCGCC 
ATGATGGTGG CCCAGTCGGC GATGGCCCTT CAGAAGTCTG GAGAGTCGAA TGTGGCTGCT 
GCTGCCGCTG CTGTGGCCGC TGCTGCAGCG GCTACCGGAA CTGCACAGCA AACGCTGGTG 
GCAATGAACC GGCAGGCCAG CGTTGCTGCA GCTGCCGCCG TGGTTGCTGC GGCCAAGGCA 
AAATCGGACT CAGATGTCGA TCTGATGGAT GTGGTTTCCG GCGGAAAGCA GCACCATTCA 
CCAAAGGCGA GCCAGGGCAG CTCTACGTCC GGGAGTACAC CGGTGCAGCC CTCGTTCAAG 
CTAAATGATG TCTGCCAGCC CGGAAACACA CTGCTTTGGG ATCTGCTTCA AGACGATAAA 
ATTGGCCAAC TAGGCGAGTC TCTGGCTCTA GAGGCGGAGA AAGCTCTGGC CACTCTCCTG 
TGCTTCAGCA TGGACCGCCA ACTGCGAACG AAATTCATCG AGGGTTGTCT GTATAACGTC 
GCCAACAACC GGAGTGTTAT TGTCAGTCTG CGACTGCTGC CCAAACTGTT CGCCTCGTTC 
CAACAGTTTC GACCCTCGGA CACGCACTCT ATGACGATGT GGGCAGAGCG AAATCACCGG 
ATGATGCAGT GCTTTTTCAA TAATATACGT CACTATGCGC GTCGCCATGC CGAGGTACTG 
ATCACCCAAA ACGGAGAGCA GCAGCAGCAG CAGCTGGGCG GACAACTCTA CTCGCACAAG 
ACACAAGTTT CTGTAAGACT GCAGTTCCTA TCATCGATAT TCTCGACGGT GGGATCCCCG 
AAAAGCTTCC GCCTAACGCT GGAGCAGCTT GACGCGCTAT GGGAGTGGCT GGCCCACGAT 
CCGGAGTGTG CCGACTGCTA CTTTTCGTGG CTGCAGGCCC AGGCCAAAGG CGGTGATCAG 
CATGCTCTGG GCATTGAAGC ACTTCAGCAT TTGTATTTGA AGAAACTCCC AGAGCTGCGA 
CCCGAGGAGT TCTCTATGGT TGCGTTGGGA CTATTTCAAC AGCTCTGTAG CTTCGCCCGC 
ATCGCCATGG CCGAGTACGA CAATCATAGC GACCAGATCT CGGCGAGCGC CAGTGCCGTT 
GGCATGTACC ATCTTTGGAA GATAGCGTTG CGTGCCCAGA GCAACGATGT CTCCTTGGCG 
GCCATACAGT ACATCAACAT GTACTACATG GGTCAACAAT TGCGCCTGGA GAAGGAGTTC 
GTTTCACAGT GTATGGAAAA CCTAGTTCAA GCGGCCACGG CTTTGGAAAG CATCGATGAT 
GAGAATGCTT TGATGCGTGT CCAGCGTGGC CTGCTCTTGC TGAACACCCA TTTGGATACG 
TTCCGGCGTC GATATGCGTT TCATCTGCGC CGCTGGGCCA TCGAAGGCAA AGGGATTGGA 
TCGCACAGTA ACCTTAAAAA TGAGGGAGCG GGTCCACCAC TACGAATAGT GCTGCAACAG 
GCAGGACTTT CGGAAAAAAG CCTTTTGCAG ATGCACGCGT GTGACTTAAT CGCCGACCTC 
AAGGCTGAGG TGTCCAAGTG GTGGGAGAGC CTTCAAACTG GACTAGCTGC TCCTGTGCTC 
GGTCTGCTGC TCAGCGATGG ACCGCTACGC ATTATCACAC AGGGACAGGA ATTGACCTCG 
GATTACGATG AACGGAGTCT CGGAGATGCT GGTTTCAAGG ATAACCAGAT CGTCTACGTA 
TCGTTAGGTG GACGTGGAGC CAGGCGAAAG GAATCCAATT TGGAGCATCC GTCAATGCTG 
CCGCCCCCAC CAAAGGAGTG TTTGCCCACG GTGCTCCTGC TGCAGCCAAA GTACTTCGAG 
AAGCTATTTT GCCTGATGCA GACCCTGGGC GATATGCAAC CGCAAGCGTC GACTGTAAAT 
CCCCAGCACC ACACAAAGGC GCAATTGCTC TCGCGACGCG TATGGGATAT CCTCGCCATG 
CTGCCAACTA ATCCGCACAT TCTAGACGCC TTTAAGAGTC TGGTTACGGA CCTAAGCGAA 
CTCGAGCAGC TTGATGCTGG CGGAGAGGAG GAGCAACTGG CCACCAAGCG CAAGCAAATC 
AAGCAGAAGT TCCGAGATCT TCTCGACCCG AACAACTTGC AAAAGTTCAT GTACTCCCTC 
CATATTGTGG AGAGTCTGGC CCTAACAAGT TCCAGACGTG GTGAATCCAA CGGCAATGTG 
GCCATGGGAA ATACACCGGA ACAGGTACGG GTGAAGAAAT CCTCGATGGG AAGACGGAGA 
AATAGCAACG AGCAGCCGCC ACCGCCGCCG CCAGAAGTCA AGATGAGCAA GGAGCAACTG 
TGCGAGCTGG AGGCCCCACT GACGCCCACA CCCTCGACTG GCTTGCAGGA TGTGGAAACG 
GAGGCCAGCA GTAGCAGCGG TGGCGACAAA GAAAACCAAC CGAAACAACA CAGCAAGCGT 
CAGAAGAAAG GCGAAACTTT TGAACAGGAA AAGGAGCGCC CAGTGGGTTG CTCCACGCCG 
CCGTCTCCAA CTCCTCCGCC ACCAGCACTT TCGGTAGTGG AAAGAGGTGA CAATAAGTGG 
AGTGAGGCGT TCGTCAAATG TGGTGGCCTT CGTCATCTGT ACGAAATCTT TAGTGAGGGC 
CAACTACAAC AAAGCGCACA TCCCAAGGAG TTGGCTTTAA ATGAATGGCG ACACGATTGC 
CTGGCCAGCC TGCTGCGCAT TCTCTGGCTT CTTGGATTCG AGGAACTGCA GTCGGCGGAT 
GCCCATGTTC TAATGTCACG TCCGCACCCG TTTATGCTAC AGTTGATGGA GGTACCACAG 
TGCCTGACCC GACTCTCATC GATCCTTAAC GACGAAGTTC ACCAGCAGCA TCAGGCTTCT 
TCGGCCAATC CACTGGTATT CCCCTATCAG TTCCAGCACT TGCGTACTGG CTTTTGGGGC 
AGGGCCCAGC TCATCCAGTT CGCTATGAAC ATTCTAGTGA GTTTTGTGCA CGCCTCAGCG 
GAAGCGAGAA GGCTTCTCTG GGCCCCAACT GGTACCGATC ACTGTCGATG GCTGCAGAAG 
TTCATCCTGG AGGATCCCGA ACCGGCGGTA CGGCGTGAGA TATGCGCTGG TCTTTATCGC 
ATTTGCTTAG GAAACGCACA TAGCTATCGC CTCTTACTAG CACCACTGCT GCACAAGCTC 
ATCGCCCTGT TGCCATTGGC CGAGCAGATG AGCTCTGGCA ACCAGCACAC GCAGTTTTTG 
CTCTCGGAAG AGGGCAAGGA TCCCTACGGA CCCGCCTGCC GTGACTACTT TTGGCTGCTG 
GCCAGATTGG TGGATACTCT CTCCCCGGAA ATGGTGGCCG AGGAGCACAT CGACATTGAA 
ATGCTGTGCG AAAGCATCTC GCAGAGCATT CTCACCCGAG AGTATTATGA ACTGCGCCAT 
GGTTACCAGG ACGATGGCTT GGTGGGTCTC CTAAATCTCA TGTCCAATTT GATCAAGTAC 
GACACCACAT TCAAGTACAC ACCCAAGGCG TTATCCTTTA TTGAGCAACT GATTGGCTTC 
CTGTTTGACA TGCCCTCGCC GGCAGATCGC CAGAAGCCCA AGTGCAAGTC GGCGTCGTCT 
CGAGCATCTG CCTACGATCT GCTGGTTGAA CTTTGCCGTG GATGCGCCAC AAACTATGCA 
TATCTGCATG GACGTCTGTT GGCACAGCAC AAATCCGGAC CCAAGCAGCC GTATCCCTGG 
GACTATTGGC CGCGCGATGA AGGCAGAGCT GAGTGCGGAT ATGTGGGGCT AACTAATTTG 
GGAGCCACTT GCTATATGGC CTCTTGCGTC CAGCACTTGT ACATGATGCC ACAGGCGCGA 
GCAGCTGTTT TGCGAGTTCC ACCCAACGCC GCCCGTAAGC ATGGGCCCAC ACTTCTGGAA 
CTACAACGGA TGTTTGCCTA CCTACTGGAG TCGGAACGGA AGTCTTACAA CCCGCGAAGC 
TTCTGCCGGG TGTACCAGAT GGATCACCAG CCGCTAAATA CGGGAGAGCA AAAGGATATG 
GCAGAGTTCT TTATCGATTT GGTGTCCAAA CTGGAAGATA TGACGCCTGA CTTGAAGCAC 
CTGGTGAAAA GGCTCTTCTG TGGATCCTTG AGCAATAATG TCGTCTCCCT GGATTGCGGT 
CACGTTTCCC GCACGGCCGA GGACTTTTAC ACGGTTCGCT GCCAGGTTGC AGATATGCGC 
AATCTGCAGG AATCCCTGGA CGAAGTCACC GTTAAGGATA CCCTCGAGGG CGACAACATG 
TACACCTGCT CGCAGTGCGG CAAAAAAGTA CGAGCCGAGA AGCGGGCTTG CTTCAAGAAG 
CTTCCCCAGA TCTTGTGCTT CAACACCATG CGCTACACCT TCAATATGGT CACCATGCTC 
AAGGAGAAGG TCAACACGCA CTTTTCCTTT CCGCTACGCC TGAACATGTG TCACTATGTG 
GAGAAGACCT TGATGCCGCA GCAGTACAAA GAGGAGCGAG AAAGGCGCCA AAAGGAGAAG 
GAAGGAGCAG ATGGTAGTGG CGATGGGAAT GACAACGAAA AGGCAGAGGC CACGCTAGAT 
GACGATATTG AAGAATGCTA CGAGTACGAA CTGGTGGGCG TCACTGTCCA CACGGGCACA 
GCGGATGGCG GCCACTACTA CAGCTTTATA AAGGAGCGGA CGAAGACTAG TTACCATACG 
CACGAGCGGT GGTTTCTTTT CAACGATGCC GAAGTGAAGC CTTTCGATCC CTCACAGATA 
GCGGCGGAGT GCTTTGGCGG CGAGATGACG AGCAAAACCT ACGACTCAGT GACGGAGAAA 
TATCTGGACT TTAGTTTCGA GAAGACCAAC TCTGCCTACA TGCTGTTCTA CGAGCGCCGA 
CTGCCAGAGC ATCTGCAGCG CCGGCATTCC GAGCTGCTGG TTACACCCAC GCCCAGTCCC 
ACAGTGGAAG AGAAGTCCGA GGCGGAGGAG CCCACTAAAA TGGAGACAAG CTCTAGCGAA 
ATCAAGGCAG ATGTGGATGT GGAAGTGGAA GTTGAGGAGA AGGACAAGGA GAAGCCTGCG 
CAGACTGACA CAGAATCTAA GGAAACGCCT GCAAAAGAGG AGATCGCGGA TGACAAATCG 
AAGCAGGATG AGCCTGAGGA AAAGAAAATC GAAAAGCAAT CCAGAGAGGG CGAAGAGAAA 
TCGGAAACGG ACGAAAAGCC CACGGAGATG ACGACCGTGA GCACTGAGGA GGAAAAGCAG 
CCAACAGCCA ATTGTGATAA TCACCAGCAA AACAACAATA GCAACAGCAA AGCCAGCAAC 
GATCAGCAAC CATCCACGTC GAAGGCTGCC CAGAAGCTAC AACTATTTCG TCCATTGTTG 
AACAAGGAGC TCGAGGATTG GATTTGGCAG GACAATCGAC AGTTCCTGCA GGATCGCAAC 
ATTTTCGAGC ACACATATTT CAACTTTATG TGGCAGATAT GTGGGCACAT ACCCCAATCA 
TTAATATCAG AGACGGACGT CACCTGCATG GCAGCCAAGT TGTCTGTGTC CTTTTTCATT 
GAGACTTTTA TTCATGCTAA GGAAAAGCCC ACTATGGTGC CCTGGGTGGA GCTGCTGACC 
AAGCAGTTCA ATGCCAGCCA AGAGGCCTGC GAGTGGTTTC TGTCGCACAT GTCGCAGGAG 
CCCTACTGGC CGGTCCAGGT GCTAATCCAG TGTCCCAACC AAATGGTGCG TCAAATGTTT 
CAGCGTTTGG TAATCCATGT CATCCAGCAA CTACGTGCCT CACATGCTCA TCTGTATCTG 
GAAGTGGAGA CAGACGAGGA TGACAAGGAG TTAATAGGCC AAGCCTCTTG CGTCACCCGT 
TTCATTGGTT CGCTCATCTC GCTCCTGGAG CACGGTGCCC GAGCGAACCT GCGGCACTTG 
TCCGAGTACT TTGGACTGTT GTGCGAGTTC TCACGGATGG GAGATGAGGA AGCCATGTAC 
TTGCTGCGCA TCGGTGTACT CAAGAGCCTG GTCGACTTCT ATCTGGGTCA CAAGCAAACA 
GACAGCATAG ACATAAGCTC TGACAACGAA GACAACTCCT CTGAAGAGGC GCTGTCCGTG 
GAGAAGATGC GTCCGGCCTC GCTGGACAAG ATGATTGCTC TGTGCGCCAG CCTAGTGGAA 
AGATCAAGAG GAGCCGACTT CCGGCTGCGA CTCTCGCCCA AAGACTTCTC TGCAATCGCC 
GGCGGCAAGG TTATACTCAA AAAGCTGGAA AGCTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

puf ( NM_001275994.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262923.1 Drosophila melanogaster puffyeye (puf), transcript variant H, mRNA.

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CloneID	OFa07738
Clone ID Related Accession (Same CDS sequence)	NM_001275994.2
Accession Version	NM_001275994.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	puffyeye, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001275994.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCGAGG TGTGCGCCGA CTTCCAGAAT CTCCTGGAGC TCTATGAGGT GCGCGTGGCC 
AGCTCGGATC TGAAGTTCCA GCTGCTGCTG AAGAGCGAGA TCGAGACCAC CTTCAACTAC 
ATCCAGTCGT GGCCGCAGCG TCAGTGCATG TGCCTGTACC GGGACACCAA GAACTATGAC 
CGTTTCAACC TGGTAGTGCA GTCGCTCATC TGTCTGACGG TGCAGCATCT GAAGCACATC 
GACCACCTCA TCGACAACTA CAAGCGGCTA ACGGCCAGTG CTGCCCAGGT GGTGGCCCAG 
GCCCAGCAGC AGCGTGAACA GCAGCGGGAC GAAGCGTCAG CCCAGGCAGA GGCAAAGGAA 
TCATCCGCGC CAGCGGAAGA ACCTAAGAAG GAGGAGCCAT CTGGGTCCGC CGGTGAGGAG 
GCTCAGGGCT CCGGCGACGG GCCAGCCAAA AAGCCGCCAG TTGGCCCCTG CACTCCCCCT 
CCCCCGCAAA CGGCCAATCC TCAGCACAAA TCCCACCTGC AGTACACAGA GGAGCCCTGG 
ATCCTGCCCG AGGTGGAGAA ACTGTTGGTG CTCGTTTCAA AGGTGTTCCT GCTCAACTTC 
CCGCTGTACA TCGCCCACAA ACATGGCATG CACTCTCGGC TGGACGACCT GCAGGCTGAG 
GAGGCCCACC ACCTGGCCCT CATCTGCGAT CTTCACGACA ACGACCTGCC CATCTATCTG 
CTGCGGAATG TGAGCCTGTT CTGCAACTCG GGCGGCTTTG GCGCCATGTC GCTGTGCTTC 
GAACATCCCG ATCTGCCAGT GTCCACCGCT CACTCGATGA CCGCTGCCGT CTCAAATGTG 
AAACTTTGGC TGAACTATCA CTGTAATACT CAGCTCTTCG TGCCGCTGCG AAGTCGGATA 
CTGCAGTACA TGTGCAAGCT GTCGGATCAG AGCCTCCGAT CTGCCGCAAC CCGCGCGATG 
GCCGATTTTG TGTGGTCCTC TATGCGAGAC CCACTGGATG TTGCTGTCAA CTTCGACACC 
GAAGGATTGG CCCTGGCCTT TAAGTACTTC ACATCGACCA CGCTAACCAT GCGACTGGCA 
GGCATGGCCC AGATCAACGC ACACATTAAT CTATTTAACG AGATCTGCAC CACCGAAACG 
GTCAACGAAG TGGAGCTGTT CGGGCAGCGC ATCGCGAACT GGCTGACTGA AAATCATATT 
GTACAGCACC TGTTTGGCCC GAATCTGCAC GTAGAAATCG TTAAACAGGC ACACGTGCTG 
CTCAACTTTT TGGCGGTAGA GAACCAGATC TCTGAAGAGG ACATCAAGCT GATCTGGCAA 
GCCACCCAGC TGAAGCACTG CTCAAAGACG ATCTTCGACA TCCTGCCTTC GCTGGTGAAG 
AATCTGACCC CGCGCCCGGC AATGCACCTT TACTCCCTCC TTTGCCGCAT GGATCCCAAG 
GAGCACACTG AGCAGAGCAT ATACATAGCC TCAGCGCTAA CAAAACAGCT GTGGACTCGT 
GATACCTCAC GCAGTCAGAT GAACCTGATG CAAGACCACC TGCTGGGGTC CAACGTCACC 
GCTTCCAGCT CTGACAGCGG CAGCATTGAG GGCAGCAACA CAGAGGACGA CCACGTGGGC 
GCCGACGACA GTAGCATTGC CAGCGGTGGC GGCGGCGGAG GTGTTGGTAA CAAGAGTCCA 
ATCGATGGAG TTACTCCATG CAAGCAGGCC CGGCACAGGC GGCACATCTG TGACCCCACC 
ACCGAGAAGG GAAAGCAAAT AAGTCCCGAG GATATGGCCA AAGTAGACTT GGTGAACAAA 
CGGATTGTGA ACATTATCGA CAACACAAGC AGTGAAGAGG AGCTGCAATC GCGGGCCGCA 
CTGGAGCTGC AACTGCATCG CAGCCGCAAA AAGACGAGCA ACAAGCGACG GCGACAAAAA 
ACCAACAAAC AGATCATTCT GCCCCATGAG CTGGTCGAGA TTTGGGACGG AGTTGAAGAT 
GTGCCCAGCA GTGACGAGGC TGATGGCGAA GCCGATGGTG ATGGCGAGGG CGAACTTTTA 
GCGGACAGCG ATGAGTGCAG CGATGGTGCT ACGAGTCAGC TGGTACCCGA CGCCGTGCTA 
AAGCATCTGC AGGGAGAGGG ACCTTTTATC AGGGCCATCG AGACCAATAT CAATGAGCTG 
CTCAGTGGTG CCGAGAACGA TGGCAGTTAC TCATCCCCGA TGAGTAACAA ATCTGAAAAG 
AACCTGGCTG ACTTCGATGA CGAGGACGTG TCGCCTTGCG AGGAGGAGCT GGCCCAGCTG 
GTCAGCTCCC GCGCCAATTG TTCGGACGTT CCACCAGCCT TTGCAGCCGC TGCCGCCGCC 
ATGATGGTGG CCCAGTCGGC GATGGCCCTT CAGAAGTCTG GAGAGTCGAA TGTGGCTGCT 
GCTGCCGCTG CTGTGGCCGC TGCTGCAGCG GCTACCGGAA CTGCACAGCA AACGCTGGTG 
GCAATGAACC GGCAGGCCAG CGTTGCTGCA GCTGCCGCCG TGGTTGCTGC GGCCAAGGCA 
AAATCGGACT CAGATGTCGA TCTGATGGAT GTGGTTTCCG GCGGAAAGCA GCACCATTCA 
CCAAAGGCGA GCCAGGGCAG CTCTACGTCC GGGAGTACAC CGGTGCAGCC CTCGTTCAAG 
CTAAATGATG TCTGCCAGCC CGGAAACACA CTGCTTTGGG ATCTGCTTCA AGACGATAAA 
ATTGGCCAAC TAGGCGAGTC TCTGGCTCTA GAGGCGGAGA AAGCTCTGGC CACTCTCCTG 
TGCTTCAGCA TGGACCGCCA ACTGCGAACG AAATTCATCG AGGGTTGTCT GTATAACGTC 
GCCAACAACC GGAGTGTTAT TGTCAGTCTG CGACTGCTGC CCAAACTGTT CGCCTCGTTC 
CAACAGTTTC GACCCTCGGA CACGCACTCT ATGACGATGT GGGCAGAGCG AAATCACCGG 
ATGATGCAGT GCTTTTTCAA TAATATACGT CACTATGCGC GTCGCCATGC CGAGGTACTG 
ATCACCCAAA ACGGAGAGCA GCAGCAGCAG CAGCTGGGCG GACAACTCTA CTCGCACAAG 
ACACAAGTTT CTGTAAGACT GCAGTTCCTA TCATCGATAT TCTCGACGGT GGGATCCCCG 
AAAAGCTTCC GCCTAACGCT GGAGCAGCTT GACGCGCTAT GGGAGTGGCT GGCCCACGAT 
CCGGAGTGTG CCGACTGCTA CTTTTCGTGG CTGCAGGCCC AGGCCAAAGG CGGTGATCAG 
CATGCTCTGG GCATTGAAGC ACTTCAGCAT TTGTATTTGA AGAAACTCCC AGAGCTGCGA 
CCCGAGGAGT TCTCTATGGT TGCGTTGGGA CTATTTCAAC AGCTCTGTAG CTTCGCCCGC 
ATCGCCATGG CCGAGTACGA CAATCATAGC GACCAGATCT CGGCGAGCGC CAGTGCCGTT 
GGCATGTACC ATCTTTGGAA GATAGCGTTG CGTGCCCAGA GCAACGATGT CTCCTTGGCG 
GCCATACAGT ACATCAACAT GTACTACATG GGTCAACAAT TGCGCCTGGA GAAGGAGTTC 
GTTTCACAGT GTATGGAAAA CCTAGTTCAA GCGGCCACGG CTTTGGAAAG CATCGATGAT 
GAGAATGCTT TGATGCGTGT CCAGCGTGGC CTGCTCTTGC TGAACACCCA TTTGGATACG 
TTCCGGCGTC GATATGCGTT TCATCTGCGC CGCTGGGCCA TCGAAGGCAA AGGGATTGGA 
TCGCACAGTA ACCTTAAAAA TGAGGGAGCG GGTCCACCAC TACGAATAGT GCTGCAACAG 
GCAGGACTTT CGGAAAAAAG CCTTTTGCAG ATGCACGCGT GTGACTTAAT CGCCGACCTC 
AAGGCTGAGG TGTCCAAGTG GTGGGAGAGC CTTCAAACTG GACTAGCTGC TCCTGTGCTC 
GGTCTGCTGC TCAGCGATGG ACCGCTACGC ATTATCACAC AGGGACAGGA ATTGACCTCG 
GATTACGATG AACGGAGTCT CGGAGATGCT GGTTTCAAGG ATAACCAGAT CGTCTACGTA 
TCGTTAGGTG GACGTGGAGC CAGGCGAAAG GAATCCAATT TGGAGCATCC GTCAATGCTG 
CCGCCCCCAC CAAAGGAGTG TTTGCCCACG GTGCTCCTGC TGCAGCCAAA GTACTTCGAG 
AAGCTATTTT GCCTGATGCA GACCCTGGGC GATATGCAAC CGCAAGCGTC GACTGTAAAT 
CCCCAGCACC ACACAAAGGC GCAATTGCTC TCGCGACGCG TATGGGATAT CCTCGCCATG 
CTGCCAACTA ATCCGCACAT TCTAGACGCC TTTAAGAGTC TGGTTACGGA CCTAAGCGAA 
CTCGAGCAGC TTGATGCTGG CGGAGAGGAG GAGCAACTGG CCACCAAGCG CAAGCAAATC 
AAGCAGAAGT TCCGAGATCT TCTCGACCCG AACAACTTGC AAAAGTTCAT GTACTCCCTC 
CATATTGTGG AGAGTCTGGC CCTAACAAGT TCCAGACGTG GTGAATCCAA CGGCAATGTG 
GCCATGGGAA ATACACCGGA ACAGGTACGG GTGAAGAAAT CCTCGATGGG AAGACGGAGA 
AATAGCAACG AGCAGCCGCC ACCGCCGCCG CCAGAAGTCA AGATGAGCAA GGAGCAACTG 
TGCGAGCTGG AGGCCCCACT GACGCCCACA CCCTCGACTG GCTTGCAGGA TGTGGAAACG 
GAGGCCAGCA GTAGCAGCGG TGGCGACAAA GAAAACCAAC CGAAACAACA CAGCAAGCGT 
CAGAAGAAAG GCGAAACTTT TGAACAGGAA AAGGAGCGCC CAGTGGGTTG CTCCACGCCG 
CCGTCTCCAA CTCCTCCGCC ACCAGCACTT TCGGTAGTGG AAAGAGGTGA CAATAAGTGG 
AGTGAGGCGT TCGTCAAATG TGGTGGCCTT CGTCATCTGT ACGAAATCTT TAGTGAGGGC 
CAACTACAAC AAAGCGCACA TCCCAAGGAG TTGGCTTTAA ATGAATGGCG ACACGATTGC 
CTGGCCAGCC TGCTGCGCAT TCTCTGGCTT CTTGGATTCG AGGAACTGCA GTCGGCGGAT 
GCCCATGTTC TAATGTCACG TCCGCACCCG TTTATGCTAC AGTTGATGGA GGTACCACAG 
TGCCTGACCC GACTCTCATC GATCCTTAAC GACGAAGTTC ACCAGCAGCA TCAGGCTTCT 
TCGGCCAATC CACTGGTATT CCCCTATCAG TTCCAGCACT TGCGTACTGG CTTTTGGGGC 
AGGGCCCAGC TCATCCAGTT CGCTATGAAC ATTCTAGTGA GTTTTGTGCA CGCCTCAGCG 
GAAGCGAGAA GGCTTCTCTG GGCCCCAACT GGTACCGATC ACTGTCGATG GCTGCAGAAG 
TTCATCCTGG AGGATCCCGA ACCGGCGGTA CGGCGTGAGA TATGCGCTGG TCTTTATCGC 
ATTTGCTTAG GAAACGCACA TAGCTATCGC CTCTTACTAG CACCACTGCT GCACAAGCTC 
ATCGCCCTGT TGCCATTGGC CGAGCAGATG AGCTCTGGCA ACCAGCACAC GCAGTTTTTG 
CTCTCGGAAG AGGGCAAGGA TCCCTACGGA CCCGCCTGCC GTGACTACTT TTGGCTGCTG 
GCCAGATTGG TGGATACTCT CTCCCCGGAA ATGGTGGCCG AGGAGCACAT CGACATTGAA 
ATGCTGTGCG AAAGCATCTC GCAGAGCATT CTCACCCGAG AGTATTATGA ACTGCGCCAT 
GGTTACCAGG ACGATGGCTT GGTGGGTCTC CTAAATCTCA TGTCCAATTT GATCAAGTAC 
GACACCACAT TCAAGTACAC ACCCAAGGCG TTATCCTTTA TTGAGCAACT GATTGGCTTC 
CTGTTTGACA TGCCCTCGCC GGCAGATCGC CAGAAGCCCA AGTGCAAGTC GGCGTCGTCT 
CGAGCATCTG CCTACGATCT GCTGGTTGAA CTTTGCCGTG GATGCGCCAC AAACTATGCA 
TATCTGCATG GACGTCTGTT GGCACAGCAC AAATCCGGAC CCAAGCAGCC GTATCCCTGG 
GACTATTGGC CGCGCGATGA AGGCAGAGCT GAGTGCGGAT ATGTGGGGCT AACTAATTTG 
GGAGCCACTT GCTATATGGC CTCTTGCGTC CAGCACTTGT ACATGATGCC ACAGGCGCGA 
GCAGCTGTTT TGCGAGTTCC ACCCAACGCC GCCCGTAAGC ATGGGCCCAC ACTTCTGGAA 
CTACAACGGA TGTTTGCCTA CCTACTGGAG TCGGAACGGA AGTCTTACAA CCCGCGAAGC 
TTCTGCCGGG TGTACCAGAT GGATCACCAG CCGCTAAATA CGGGAGAGCA AAAGGATATG 
GCAGAGTTCT TTATCGATTT GGTGTCCAAA CTGGAAGATA TGACGCCTGA CTTGAAGCAC 
CTGGTGAAAA GGCTCTTCTG TGGATCCTTG AGCAATAATG TCGTCTCCCT GGATTGCGGT 
CACGTTTCCC GCACGGCCGA GGACTTTTAC ACGGTTCGCT GCCAGGTTGC AGATATGCGC 
AATCTGCAGG AATCCCTGGA CGAAGTCACC GTTAAGGATA CCCTCGAGGG CGACAACATG 
TACACCTGCT CGCAGTGCGG CAAAAAAGTA CGAGCCGAGA AGCGGGCTTG CTTCAAGAAG 
CTTCCCCAGA TCTTGTGCTT CAACACCATG CGCTACACCT TCAATATGGT CACCATGCTC 
AAGGAGAAGG TCAACACGCA CTTTTCCTTT CCGCTACGCC TGAACATGTG TCACTATGTG 
GAGAAGACCT TGATGCCGCA GCAGTACAAA GAGGAGCGAG AAAGGCGCCA AAAGGAGAAG 
GAAGGAGCAG ATGGTAGTGG CGATGGGAAT GACAACGAAA AGGCAGAGGC CACGCTAGAT 
GACGATATTG AAGAATGCTA CGAGTACGAA CTGGTGGGCG TCACTGTCCA CACGGGCACA 
GCGGATGGCG GCCACTACTA CAGCTTTATA AAGGAGCGGA CGAAGACTAG TTACCATACG 
CACGAGCGGT GGTTTCTTTT CAACGATGCC GAAGTGAAGC CTTTCGATCC CTCACAGATA 
GCGGCGGAGT GCTTTGGCGG CGAGATGACG AGCAAAACCT ACGACTCAGT GACGGAGAAA 
TATCTGGACT TTAGTTTCGA GAAGACCAAC TCTGCCTACA TGCTGTTCTA CGAGCGCCGA 
CTGCCAGAGC ATCTGCAGCG CCGGCATTCC GAGCTGCTGG TTACACCCAC GCCCAGTCCC 
ACAGTGGAAG AGAAGTCCGA GGCGGAGGAG CCCACTAAAA TGGAGACAAG CTCTAGCGAA 
ATCAAGGCAG ATGTGGATGT GGAAGTGGAA GTTGAGGAGA AGGACAAGGA GAAGCCTGCG 
CAGACTGACA CAGAATCTAA GGAAACGCCT GCAAAAGAGG AGATCGCGGA TGACAAATCG 
AAGCAGGATG AGCCTGAGGA AAAGAAAATC GAAAAGCAAT CCAGAGAGGG CGAAGAGAAA 
TCGGAAACGG ACGAAAAGCC CACGGAGATG ACGACCGTGA GCACTGAGGA GGAAAAGCAG 
CCAACAGCCA ATTGTGATAA TCACCAGCAA AACAACAATA GCAACAGCAA AGCCAGCAAC 
GATCAGCAAC CATCCACGTC GAAGGCTGCC CAGAAGCTAC AACTATTTCG TCCATTGTTG 
AACAAGGAGC TCGAGGATTG GATTTGGCAG GACAATCGAC AGTTCCTGCA GGATCGCAAC 
ATTTTCGAGC ACACATATTT CAACTTTATG TGGCAGATAT GTGGGCACAT ACCCCAATCA 
TTAATATCAG AGACGGACGT CACCTGCATG GCAGCCAAGT TGTCTGTGTC CTTTTTCATT 
GAGACTTTTA TTCATGCTAA GGAAAAGCCC ACTATGGTGC CCTGGGTGGA GCTGCTGACC 
AAGCAGTTCA ATGCCAGCCA AGAGGCCTGC GAGTGGTTTC TGTCGCACAT GTCGCAGGAG 
CCCTACTGGC CGGTCCAGGT GCTAATCCAG TGTCCCAACC AAATGGTGCG TCAAATGTTT 
CAGCGTTTGG TAATCCATGT CATCCAGCAA CTACGTGCCT CACATGCTCA TCTGTATCTG 
GAAGTGGAGA CAGACGAGGA TGACAAGGAG TTAATAGGCC AAGCCTCTTG CGTCACCCGT 
TTCATTGGTT CGCTCATCTC GCTCCTGGAG CACGGTGCCC GAGCGAACCT GCGGCACTTG 
TCCGAGTACT TTGGACTGTT GTGCGAGTTC TCACGGATGG GAGATGAGGA AGCCATGTAC 
TTGCTGCGCA TCGGTGTACT CAAGAGCCTG GTCGACTTCT ATCTGGGTCA CAAGCAAACA 
GACAGCATAG ACATAAGCTC TGACAACGAA GACAACTCCT CTGAAGAGGC GCTGTCCGTG 
GAGAAGATGC GTCCGGCCTC GCTGGACAAG ATGATTGCTC TGTGCGCCAG CCTAGTGGAA 
AGATCAAGAG GAGCCGACTT CCGGCTGCGA CTCTCGCCCA AAGACTTCTC TGCAATCGCC 
GGCGGCAAGG GTTTTCCATT TTTGTACCAA CAAATAAAGG ACGGCATTAA TCCGCACCAG 
ACAAAGCATT TAATACACGC GCTTTGTCGC TGGGATGAGC GCCTGGCCAC GCAGATCATT 
GGCATGCTCT TCGCGTCGGT AACCAAGCAC ACGGAGCTGT GCGCGCCATT CTTCAAGCTG 
CTAACTCTGC TCACCGAGAC GCAGGGCGGT CCAGTGGGCC TGCCTTGTTT CACCCAGTTG 
ATCCTGCCAC GAATGTGGGA CGCCGCCGAG TACTGTCCAC AGTCGGTGCT CGACTGGCTC 
TCGCTGCAGG CCACCAAGAA CAAGATCGCC CACGCCTGGA TACTTCAATC GGCCGAGAAG 
TGGCTCGAGC AATTCCTGCT GGCCCACGAT AACACTCGTG TTCGGAACGC TGCCGCCTTT 
CTGCTGGTGG CACTGGTACC CTCCCAGCCA TTCCGGGCCA ACTTCCGCGC CCACTCGCAA 
CACAAGCTGC TGGCCCTCAA TCCGCACAGT TATCGCGACA TAAATAGCGA TGCCCAGGCG 
GTGCTGCACC AGGTGATCAC GCTGCTCCTG CGATTGCTGC GTCCAGCGCG CGTCTATGCG 
GATATCGGAG CCCATGGCAC GACCAAGCTG ACCGCGTACT TTAATCTGCT CAGCTATTGT 
ATGGTTTCAA AGACTGAAAA ACTAATGGCA AGTTCGTACA TGCGCTCCCT GTGGGAGCTG 
TTCCATCCAC GCCTCTCGGA GCCCAGTGTT CCGGCCCATC ACAATAAGCA CGCACTATTA 
ACCTTCTGGC ACCACTCACT GGTGGACTGT CCGGAGAATG CTGCCCAGGT GGCCAACTGC 
CCGGAGATTA CGCGCAACAT TGCATTTAAC TATATACTGG CCGATCATGA TGACGCGGAG 
ATAGTCACCT ACAACCGTTC CATGCTGCCC GCCTATTATG GACTACTTCG TCTGTGCTGC 
GAGCAGAGCC GTGCACTCAC ACGCCAATTG TCCCAGCATC AGAATCTTCA GTGGGCATTT 
AAAAACATCA CACCGCATCC CACGCAGTAC GCGGCCGCCG TGGACGAGCT TTTCAAGTTG 
ATGGCTCTGT TCGCCACCCG TCATCCGGAT GCTAGTGAAC AGGAAAAGCT TGATGTGACC 
CAGTTTAGGA GGGCTGTGAT TGTGTCCTAC ACCAGTAGTC TGGATGCACG TGTCTCTTGG 
TCCACGCTGA TCAGTGCTCT TAAGATACTC GTGGACAATG AGGAGGACCG CACGATGGTG 
ATCTTCAACG GCGGCATTGA GATGTGTTTC GAGGCGCTGC ACACGCTACA TTCCATGCAC 
CACGAGGCCA CCGCCTGCCA CGTGGCCGGA GACCTATTCG ATCTCTTGGG CGAGATGCTA 
CTCCTCCTAG CCACTCTTCG AACCCGCACA GACAGTCCTG CTCAAAAGAA GCAGCAGCAA 
CAACAGCAGC AGCTGGAGCA ACAGTTGCAG TTGCAGCAGC AACAGATGCT GCAGAAACAA 
CAGCAGCAGT CGCAAAGCCA AACGCAAACA CCGCAGTCGC CGCAGCAAAA GGAAAAGCAG 
CTGCAGCAAC AAATGCAGCA GCATCTGCAG CTGCAACAGT TGCAGCAAAT GCAGTTCCAG 
CAGCAACACT TCCTGCGCCA GCAACATCAA CACTCGGCGC TGGCAAAGGC ACTGCCGGAT 
GCAGTGAAGC GACTGGCTAC GCTACTCAAC ACCTTCAATT CGCCAGAGAT CAGTCGCATG 
GCCTTGGAGG TGCTCAAGGA GCTGGTGCGC AATCCCAGCC TGGAAACCAT TAGTATTCTG 
GCGCCCATTC TTATCAACTG TCATCTGTCC GTGGCGAATG CTCCCAATGC GATCGGTCCT 
CTGGGACCGT ACTTTCCGCG TCGCGGTGCG AAGCATACGC CTTGGCCATT GGGAGCCAAG 
AACTCACCCC GTCCACCGCG GCCCATGGTA CAGATGTGCA TCGCTCTGGC TGAGCTGACA 
CCACGCGGTC TCGATGCCGA TTACGACGTT CAGGTGGAGT CCTTTTACCG ACCCTACCAT 
GACTTTATCG ATGTCATGAT GCGCATGTGC GTCAACACGG GCACTCTGAA CGACACGCTA 
GTCAAGCTGC AATGCTTGGT GGCCATCGAG TCCACGCCGC TGCACTTCAC CTATTTCCCC 
AAATTCTGGG TGGGCATACA CAACAATGCT CTGACGCACA AATACGTTGA GCTGCTGGTG 
AAAAATCAAC TGTTGGTAGA GTACCTTCAC AATGTCCTTA GGGACGAGCG AAGCATGTTG 
AAGGATGCCT GCGTTCGTGA ATTTCTGGAG CTCTATTATC ACAAGGTTGC CGCCCAGCTG 
CCAGTGGCCC GTATGATCTA CACAATCAAC TATGGTATGC ACTCCAAGGA CGACATTGAT 
GAGCTGTGTG GCGATCTGTT CGCCATCCGA ATCATTGCCC AGGCCACTGG AGTGCCGGCC 
TCAGTGCGCA AGGAGTTGCG AGGATCGCTG AGGGCGCTTC AGAACAAGAG CGAGCGGTTT 
AGGAAGGAAT CGGAGAGGGA TCCATTTCCG AACAAGAAGC AGAAACGTGA CTCGCAAAAG 
ATCAAGGAAA AGGAGCACCC TCAGCCGGAG AGCGAGAAAG AGACCTCAAC GGAGAACGAC 
AAACCATCAG ATGTTTCCAT GGAATCCAGC GGTAACGCGG AGCAAGCAAC GGACAGCACA 
AAACCACCAA CTCCTGCAGG CAGCGACGAT GAGCAGATGG ACAAAACTTC AGTTCCCTCG 
TCCGATGACG AGACAGAGTT GGAGGACGAG CTGCAGCCGA CGCCCAAGCG CAACAAGAAG 
GCTTCGAATA AGAAGACCGC CCAGGATCGT GTCAACGAGG AGCGCGAGAA GCGACTTATC 
ACGCTGATCA CCATGGAATC ATATATCCAG AGCATTTTTG CCATCCTTAA GAGAGATGCC 
AGTGTCCCCA GTTCGGGAGC GACTAAGGAG CCCAGTGAGG AACCGTGTGG CGAGGCATCC 
ACTTCGGCGG CTGCCGCTGT AAAGTTATCT CGTCCCAAAG GAGCCTGTAC TCCGCCGGAG 
CCAATTACCG ACGTAAACGA TACGCGTTGC AACATAGATA CTGAGACAGA GGCGGAGGCG 
GATGAGCAAT CTCCAAAGAC GAGCCAGACA AATGGTAGCC AACAGAACGA ATCCCCGCCA 
GCGGCGACCA GTGCAGACAC TGCACCCGCG AATCCTTCAC CAGCTCCCGC GGCCGCCGTT 
GCGTCCACAT CCCAGGCGGC AAGTCCAACA CAGATTTAGT TGTCATTCAA CGATTTGCTC 
AGCATTTTGT TTTTCGGCAA GCCATTGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262923.1
CDS	358..12150
Translation	MCEVCADFQNLLELYEVRVASSDLKFQLLLKSEIETTFNYIQSWPQRQCMCLYRDTKNYDRFNLVVQSLICLTVQHLKHIDHLIDNYKRLTASAAQVVAQAQQQREQQRDEASAQAEAKESSAPAEEPKKEEPSGSAGEEAQGSGDGPAKKPPVGPCTPPPPQTANPQHKSHLQYTEEPWILPEVEKLLVLVSKVFLLNFPLYIAHKHGMHSRLDDLQAEEAHHLALICDLHDNDLPIYLLRNVSLFCNSGGFGAMSLCFEHPDLPVSTAHSMTAAVSNVKLWLNYHCNTQLFVPLRSRILQYMCKLSDQSLRSAATRAMADFVWSSMRDPLDVAVNFDTEGLALAFKYFTSTTLTMRLAGMAQINAHINLFNEICTTETVNEVELFGQRIANWLTENHIVQHLFGPNLHVEIVKQAHVLLNFLAVENQISEEDIKLIWQATQLKHCSKTIFDILPSLVKNLTPRPAMHLYSLLCRMDPKEHTEQSIYIASALTKQLWTRDTSRSQMNLMQDHLLGSNVTASSSDSGSIEGSNTEDDHVGADDSSIASGGGGGGVGNKSPIDGVTPCKQARHRRHICDPTTEKGKQISPEDMAKVDLVNKRIVNIIDNTSSEEELQSRAALELQLHRSRKKTSNKRRRQKTNKQIILPHELVEIWDGVEDVPSSDEADGEADGDGEGELLADSDECSDGATSQLVPDAVLKHLQGEGPFIRAIETNINELLSGAENDGSYSSPMSNKSEKNLADFDDEDVSPCEEELAQLVSSRANCSDVPPAFAAAAAAMMVAQSAMALQKSGESNVAAAAAAVAAAAAATGTAQQTLVAMNRQASVAAAAAVVAAAKAKSDSDVDLMDVVSGGKQHHSPKASQGSSTSGSTPVQPSFKLNDVCQPGNTLLWDLLQDDKIGQLGESLALEAEKALATLLCFSMDRQLRTKFIEGCLYNVANNRSVIVSLRLLPKLFASFQQFRPSDTHSMTMWAERNHRMMQCFFNNIRHYARRHAEVLITQNGEQQQQQLGGQLYSHKTQVSVRLQFLSSIFSTVGSPKSFRLTLEQLDALWEWLAHDPECADCYFSWLQAQAKGGDQHALGIEALQHLYLKKLPELRPEEFSMVALGLFQQLCSFARIAMAEYDNHSDQISASASAVGMYHLWKIALRAQSNDVSLAAIQYINMYYMGQQLRLEKEFVSQCMENLVQAATALESIDDENALMRVQRGLLLLNTHLDTFRRRYAFHLRRWAIEGKGIGSHSNLKNEGAGPPLRIVLQQAGLSEKSLLQMHACDLIADLKAEVSKWWESLQTGLAAPVLGLLLSDGPLRIITQGQELTSDYDERSLGDAGFKDNQIVYVSLGGRGARRKESNLEHPSMLPPPPKECLPTVLLLQPKYFEKLFCLMQTLGDMQPQASTVNPQHHTKAQLLSRRVWDILAMLPTNPHILDAFKSLVTDLSELEQLDAGGEEEQLATKRKQIKQKFRDLLDPNNLQKFMYSLHIVESLALTSSRRGESNGNVAMGNTPEQVRVKKSSMGRRRNSNEQPPPPPPEVKMSKEQLCELEAPLTPTPSTGLQDVETEASSSSGGDKENQPKQHSKRQKKGETFEQEKERPVGCSTPPSPTPPPPALSVVERGDNKWSEAFVKCGGLRHLYEIFSEGQLQQSAHPKELALNEWRHDCLASLLRILWLLGFEELQSADAHVLMSRPHPFMLQLMEVPQCLTRLSSILNDEVHQQHQASSANPLVFPYQFQHLRTGFWGRAQLIQFAMNILVSFVHASAEARRLLWAPTGTDHCRWLQKFILEDPEPAVRREICAGLYRICLGNAHSYRLLLAPLLHKLIALLPLAEQMSSGNQHTQFLLSEEGKDPYGPACRDYFWLLARLVDTLSPEMVAEEHIDIEMLCESISQSILTREYYELRHGYQDDGLVGLLNLMSNLIKYDTTFKYTPKALSFIEQLIGFLFDMPSPADRQKPKCKSASSRASAYDLLVELCRGCATNYAYLHGRLLAQHKSGPKQPYPWDYWPRDEGRAECGYVGLTNLGATCYMASCVQHLYMMPQARAAVLRVPPNAARKHGPTLLELQRMFAYLLESERKSYNPRSFCRVYQMDHQPLNTGEQKDMAEFFIDLVSKLEDMTPDLKHLVKRLFCGSLSNNVVSLDCGHVSRTAEDFYTVRCQVADMRNLQESLDEVTVKDTLEGDNMYTCSQCGKKVRAEKRACFKKLPQILCFNTMRYTFNMVTMLKEKVNTHFSFPLRLNMCHYVEKTLMPQQYKEERERRQKEKEGADGSGDGNDNEKAEATLDDDIEECYEYELVGVTVHTGTADGGHYYSFIKERTKTSYHTHERWFLFNDAEVKPFDPSQIAAECFGGEMTSKTYDSVTEKYLDFSFEKTNSAYMLFYERRLPEHLQRRHSELLVTPTPSPTVEEKSEAEEPTKMETSSSEIKADVDVEVEVEEKDKEKPAQTDTESKETPAKEEIADDKSKQDEPEEKKIEKQSREGEEKSETDEKPTEMTTVSTEEEKQPTANCDNHQQNNNSNSKASNDQQPSTSKAAQKLQLFRPLLNKELEDWIWQDNRQFLQDRNIFEHTYFNFMWQICGHIPQSLISETDVTCMAAKLSVSFFIETFIHAKEKPTMVPWVELLTKQFNASQEACEWFLSHMSQEPYWPVQVLIQCPNQMVRQMFQRLVIHVIQQLRASHAHLYLEVETDEDDKELIGQASCVTRFIGSLISLLEHGARANLRHLSEYFGLLCEFSRMGDEEAMYLLRIGVLKSLVDFYLGHKQTDSIDISSDNEDNSSEEALSVEKMRPASLDKMIALCASLVERSRGADFRLRLSPKDFSAIAGGKGFPFLYQQIKDGINPHQTKHLIHALCRWDERLATQIIGMLFASVTKHTELCAPFFKLLTLLTETQGGPVGLPCFTQLILPRMWDAAEYCPQSVLDWLSLQATKNKIAHAWILQSAEKWLEQFLLAHDNTRVRNAAAFLLVALVPSQPFRANFRAHSQHKLLALNPHSYRDINSDAQAVLHQVITLLLRLLRPARVYADIGAHGTTKLTAYFNLLSYCMVSKTEKLMASSYMRSLWELFHPRLSEPSVPAHHNKHALLTFWHHSLVDCPENAAQVANCPEITRNIAFNYILADHDDAEIVTYNRSMLPAYYGLLRLCCEQSRALTRQLSQHQNLQWAFKNITPHPTQYAAAVDELFKLMALFATRHPDASEQEKLDVTQFRRAVIVSYTSSLDARVSWSTLISALKILVDNEEDRTMVIFNGGIEMCFEALHTLHSMHHEATACHVAGDLFDLLGEMLLLLATLRTRTDSPAQKKQQQQQQQLEQQLQLQQQQMLQKQQQQSQSQTQTPQSPQQKEKQLQQQMQQHLQLQQLQQMQFQQQHFLRQQHQHSALAKALPDAVKRLATLLNTFNSPEISRMALEVLKELVRNPSLETISILAPILINCHLSVANAPNAIGPLGPYFPRRGAKHTPWPLGAKNSPRPPRPMVQMCIALAELTPRGLDADYDVQVESFYRPYHDFIDVMMRMCVNTGTLNDTLVKLQCLVAIESTPLHFTYFPKFWVGIHNNALTHKYVELLVKNQLLVEYLHNVLRDERSMLKDACVREFLELYYHKVAAQLPVARMIYTINYGMHSKDDIDELCGDLFAIRIIAQATGVPASVRKELRGSLRALQNKSERFRKESERDPFPNKKQKRDSQKIKEKEHPQPESEKETSTENDKPSDVSMESSGNAEQATDSTKPPTPAGSDDEQMDKTSVPSSDDETELEDELQPTPKRNKKASNKKTAQDRVNEEREKRLITLITMESYIQSIFAILKRDASVPSSGATKEPSEEPCGEASTSAAAAVKLSRPKGACTPPEPITDVNDTRCNIDTETEAEADEQSPKTSQTNGSQQNESPPAATSADTAPANPSPAPAAAVASTSQAASPTQI*LSFNDLLSILFFGKPLD

Target ORF information:

RefSeq Version	NM_001275994.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster puffyeye (puf), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275994.2

ORF Insert Sequence:

ATGTGCGAGG TGTGCGCCGA CTTCCAGAAT CTCCTGGAGC TCTATGAGGT GCGCGTGGCC 
AGCTCGGATC TGAAGTTCCA GCTGCTGCTG AAGAGCGAGA TCGAGACCAC CTTCAACTAC 
ATCCAGTCGT GGCCGCAGCG TCAGTGCATG TGCCTGTACC GGGACACCAA GAACTATGAC 
CGTTTCAACC TGGTAGTGCA GTCGCTCATC TGTCTGACGG TGCAGCATCT GAAGCACATC 
GACCACCTCA TCGACAACTA CAAGCGGCTA ACGGCCAGTG CTGCCCAGGT GGTGGCCCAG 
GCCCAGCAGC AGCGTGAACA GCAGCGGGAC GAAGCGTCAG CCCAGGCAGA GGCAAAGGAA 
TCATCCGCGC CAGCGGAAGA ACCTAAGAAG GAGGAGCCAT CTGGGTCCGC CGGTGAGGAG 
GCTCAGGGCT CCGGCGACGG GCCAGCCAAA AAGCCGCCAG TTGGCCCCTG CACTCCCCCT 
CCCCCGCAAA CGGCCAATCC TCAGCACAAA TCCCACCTGC AGTACACAGA GGAGCCCTGG 
ATCCTGCCCG AGGTGGAGAA ACTGTTGGTG CTCGTTTCAA AGGTGTTCCT GCTCAACTTC 
CCGCTGTACA TCGCCCACAA ACATGGCATG CACTCTCGGC TGGACGACCT GCAGGCTGAG 
GAGGCCCACC ACCTGGCCCT CATCTGCGAT CTTCACGACA ACGACCTGCC CATCTATCTG 
CTGCGGAATG TGAGCCTGTT CTGCAACTCG GGCGGCTTTG GCGCCATGTC GCTGTGCTTC 
GAACATCCCG ATCTGCCAGT GTCCACCGCT CACTCGATGA CCGCTGCCGT CTCAAATGTG 
AAACTTTGGC TGAACTATCA CTGTAATACT CAGCTCTTCG TGCCGCTGCG AAGTCGGATA 
CTGCAGTACA TGTGCAAGCT GTCGGATCAG AGCCTCCGAT CTGCCGCAAC CCGCGCGATG 
GCCGATTTTG TGTGGTCCTC TATGCGAGAC CCACTGGATG TTGCTGTCAA CTTCGACACC 
GAAGGATTGG CCCTGGCCTT TAAGTACTTC ACATCGACCA CGCTAACCAT GCGACTGGCA 
GGCATGGCCC AGATCAACGC ACACATTAAT CTATTTAACG AGATCTGCAC CACCGAAACG 
GTCAACGAAG TGGAGCTGTT CGGGCAGCGC ATCGCGAACT GGCTGACTGA AAATCATATT 
GTACAGCACC TGTTTGGCCC GAATCTGCAC GTAGAAATCG TTAAACAGGC ACACGTGCTG 
CTCAACTTTT TGGCGGTAGA GAACCAGATC TCTGAAGAGG ACATCAAGCT GATCTGGCAA 
GCCACCCAGC TGAAGCACTG CTCAAAGACG ATCTTCGACA TCCTGCCTTC GCTGGTGAAG 
AATCTGACCC CGCGCCCGGC AATGCACCTT TACTCCCTCC TTTGCCGCAT GGATCCCAAG 
GAGCACACTG AGCAGAGCAT ATACATAGCC TCAGCGCTAA CAAAACAGCT GTGGACTCGT 
GATACCTCAC GCAGTCAGAT GAACCTGATG CAAGACCACC TGCTGGGGTC CAACGTCACC 
GCTTCCAGCT CTGACAGCGG CAGCATTGAG GGCAGCAACA CAGAGGACGA CCACGTGGGC 
GCCGACGACA GTAGCATTGC CAGCGGTGGC GGCGGCGGAG GTGTTGGTAA CAAGAGTCCA 
ATCGATGGAG TTACTCCATG CAAGCAGGCC CGGCACAGGC GGCACATCTG TGACCCCACC 
ACCGAGAAGG GAAAGCAAAT AAGTCCCGAG GATATGGCCA AAGTAGACTT GGTGAACAAA 
CGGATTGTGA ACATTATCGA CAACACAAGC AGTGAAGAGG AGCTGCAATC GCGGGCCGCA 
CTGGAGCTGC AACTGCATCG CAGCCGCAAA AAGACGAGCA ACAAGCGACG GCGACAAAAA 
ACCAACAAAC AGATCATTCT GCCCCATGAG CTGGTCGAGA TTTGGGACGG AGTTGAAGAT 
GTGCCCAGCA GTGACGAGGC TGATGGCGAA GCCGATGGTG ATGGCGAGGG CGAACTTTTA 
GCGGACAGCG ATGAGTGCAG CGATGGTGCT ACGAGTCAGC TGGTACCCGA CGCCGTGCTA 
AAGCATCTGC AGGGAGAGGG ACCTTTTATC AGGGCCATCG AGACCAATAT CAATGAGCTG 
CTCAGTGGTG CCGAGAACGA TGGCAGTTAC TCATCCCCGA TGAGTAACAA ATCTGAAAAG 
AACCTGGCTG ACTTCGATGA CGAGGACGTG TCGCCTTGCG AGGAGGAGCT GGCCCAGCTG 
GTCAGCTCCC GCGCCAATTG TTCGGACGTT CCACCAGCCT TTGCAGCCGC TGCCGCCGCC 
ATGATGGTGG CCCAGTCGGC GATGGCCCTT CAGAAGTCTG GAGAGTCGAA TGTGGCTGCT 
GCTGCCGCTG CTGTGGCCGC TGCTGCAGCG GCTACCGGAA CTGCACAGCA AACGCTGGTG 
GCAATGAACC GGCAGGCCAG CGTTGCTGCA GCTGCCGCCG TGGTTGCTGC GGCCAAGGCA 
AAATCGGACT CAGATGTCGA TCTGATGGAT GTGGTTTCCG GCGGAAAGCA GCACCATTCA 
CCAAAGGCGA GCCAGGGCAG CTCTACGTCC GGGAGTACAC CGGTGCAGCC CTCGTTCAAG 
CTAAATGATG TCTGCCAGCC CGGAAACACA CTGCTTTGGG ATCTGCTTCA AGACGATAAA 
ATTGGCCAAC TAGGCGAGTC TCTGGCTCTA GAGGCGGAGA AAGCTCTGGC CACTCTCCTG 
TGCTTCAGCA TGGACCGCCA ACTGCGAACG AAATTCATCG AGGGTTGTCT GTATAACGTC 
GCCAACAACC GGAGTGTTAT TGTCAGTCTG CGACTGCTGC CCAAACTGTT CGCCTCGTTC 
CAACAGTTTC GACCCTCGGA CACGCACTCT ATGACGATGT GGGCAGAGCG AAATCACCGG 
ATGATGCAGT GCTTTTTCAA TAATATACGT CACTATGCGC GTCGCCATGC CGAGGTACTG 
ATCACCCAAA ACGGAGAGCA GCAGCAGCAG CAGCTGGGCG GACAACTCTA CTCGCACAAG 
ACACAAGTTT CTGTAAGACT GCAGTTCCTA TCATCGATAT TCTCGACGGT GGGATCCCCG 
AAAAGCTTCC GCCTAACGCT GGAGCAGCTT GACGCGCTAT GGGAGTGGCT GGCCCACGAT 
CCGGAGTGTG CCGACTGCTA CTTTTCGTGG CTGCAGGCCC AGGCCAAAGG CGGTGATCAG 
CATGCTCTGG GCATTGAAGC ACTTCAGCAT TTGTATTTGA AGAAACTCCC AGAGCTGCGA 
CCCGAGGAGT TCTCTATGGT TGCGTTGGGA CTATTTCAAC AGCTCTGTAG CTTCGCCCGC 
ATCGCCATGG CCGAGTACGA CAATCATAGC GACCAGATCT CGGCGAGCGC CAGTGCCGTT 
GGCATGTACC ATCTTTGGAA GATAGCGTTG CGTGCCCAGA GCAACGATGT CTCCTTGGCG 
GCCATACAGT ACATCAACAT GTACTACATG GGTCAACAAT TGCGCCTGGA GAAGGAGTTC 
GTTTCACAGT GTATGGAAAA CCTAGTTCAA GCGGCCACGG CTTTGGAAAG CATCGATGAT 
GAGAATGCTT TGATGCGTGT CCAGCGTGGC CTGCTCTTGC TGAACACCCA TTTGGATACG 
TTCCGGCGTC GATATGCGTT TCATCTGCGC CGCTGGGCCA TCGAAGGCAA AGGGATTGGA 
TCGCACAGTA ACCTTAAAAA TGAGGGAGCG GGTCCACCAC TACGAATAGT GCTGCAACAG 
GCAGGACTTT CGGAAAAAAG CCTTTTGCAG ATGCACGCGT GTGACTTAAT CGCCGACCTC 
AAGGCTGAGG TGTCCAAGTG GTGGGAGAGC CTTCAAACTG GACTAGCTGC TCCTGTGCTC 
GGTCTGCTGC TCAGCGATGG ACCGCTACGC ATTATCACAC AGGGACAGGA ATTGACCTCG 
GATTACGATG AACGGAGTCT CGGAGATGCT GGTTTCAAGG ATAACCAGAT CGTCTACGTA 
TCGTTAGGTG GACGTGGAGC CAGGCGAAAG GAATCCAATT TGGAGCATCC GTCAATGCTG 
CCGCCCCCAC CAAAGGAGTG TTTGCCCACG GTGCTCCTGC TGCAGCCAAA GTACTTCGAG 
AAGCTATTTT GCCTGATGCA GACCCTGGGC GATATGCAAC CGCAAGCGTC GACTGTAAAT 
CCCCAGCACC ACACAAAGGC GCAATTGCTC TCGCGACGCG TATGGGATAT CCTCGCCATG 
CTGCCAACTA ATCCGCACAT TCTAGACGCC TTTAAGAGTC TGGTTACGGA CCTAAGCGAA 
CTCGAGCAGC TTGATGCTGG CGGAGAGGAG GAGCAACTGG CCACCAAGCG CAAGCAAATC 
AAGCAGAAGT TCCGAGATCT TCTCGACCCG AACAACTTGC AAAAGTTCAT GTACTCCCTC 
CATATTGTGG AGAGTCTGGC CCTAACAAGT TCCAGACGTG GTGAATCCAA CGGCAATGTG 
GCCATGGGAA ATACACCGGA ACAGGTACGG GTGAAGAAAT CCTCGATGGG AAGACGGAGA 
AATAGCAACG AGCAGCCGCC ACCGCCGCCG CCAGAAGTCA AGATGAGCAA GGAGCAACTG 
TGCGAGCTGG AGGCCCCACT GACGCCCACA CCCTCGACTG GCTTGCAGGA TGTGGAAACG 
GAGGCCAGCA GTAGCAGCGG TGGCGACAAA GAAAACCAAC CGAAACAACA CAGCAAGCGT 
CAGAAGAAAG GCGAAACTTT TGAACAGGAA AAGGAGCGCC CAGTGGGTTG CTCCACGCCG 
CCGTCTCCAA CTCCTCCGCC ACCAGCACTT TCGGTAGTGG AAAGAGGTGA CAATAAGTGG 
AGTGAGGCGT TCGTCAAATG TGGTGGCCTT CGTCATCTGT ACGAAATCTT TAGTGAGGGC 
CAACTACAAC AAAGCGCACA TCCCAAGGAG TTGGCTTTAA ATGAATGGCG ACACGATTGC 
CTGGCCAGCC TGCTGCGCAT TCTCTGGCTT CTTGGATTCG AGGAACTGCA GTCGGCGGAT 
GCCCATGTTC TAATGTCACG TCCGCACCCG TTTATGCTAC AGTTGATGGA GGTACCACAG 
TGCCTGACCC GACTCTCATC GATCCTTAAC GACGAAGTTC ACCAGCAGCA TCAGGCTTCT 
TCGGCCAATC CACTGGTATT CCCCTATCAG TTCCAGCACT TGCGTACTGG CTTTTGGGGC 
AGGGCCCAGC TCATCCAGTT CGCTATGAAC ATTCTAGTGA GTTTTGTGCA CGCCTCAGCG 
GAAGCGAGAA GGCTTCTCTG GGCCCCAACT GGTACCGATC ACTGTCGATG GCTGCAGAAG 
TTCATCCTGG AGGATCCCGA ACCGGCGGTA CGGCGTGAGA TATGCGCTGG TCTTTATCGC 
ATTTGCTTAG GAAACGCACA TAGCTATCGC CTCTTACTAG CACCACTGCT GCACAAGCTC 
ATCGCCCTGT TGCCATTGGC CGAGCAGATG AGCTCTGGCA ACCAGCACAC GCAGTTTTTG 
CTCTCGGAAG AGGGCAAGGA TCCCTACGGA CCCGCCTGCC GTGACTACTT TTGGCTGCTG 
GCCAGATTGG TGGATACTCT CTCCCCGGAA ATGGTGGCCG AGGAGCACAT CGACATTGAA 
ATGCTGTGCG AAAGCATCTC GCAGAGCATT CTCACCCGAG AGTATTATGA ACTGCGCCAT 
GGTTACCAGG ACGATGGCTT GGTGGGTCTC CTAAATCTCA TGTCCAATTT GATCAAGTAC 
GACACCACAT TCAAGTACAC ACCCAAGGCG TTATCCTTTA TTGAGCAACT GATTGGCTTC 
CTGTTTGACA TGCCCTCGCC GGCAGATCGC CAGAAGCCCA AGTGCAAGTC GGCGTCGTCT 
CGAGCATCTG CCTACGATCT GCTGGTTGAA CTTTGCCGTG GATGCGCCAC AAACTATGCA 
TATCTGCATG GACGTCTGTT GGCACAGCAC AAATCCGGAC CCAAGCAGCC GTATCCCTGG 
GACTATTGGC CGCGCGATGA AGGCAGAGCT GAGTGCGGAT ATGTGGGGCT AACTAATTTG 
GGAGCCACTT GCTATATGGC CTCTTGCGTC CAGCACTTGT ACATGATGCC ACAGGCGCGA 
GCAGCTGTTT TGCGAGTTCC ACCCAACGCC GCCCGTAAGC ATGGGCCCAC ACTTCTGGAA 
CTACAACGGA TGTTTGCCTA CCTACTGGAG TCGGAACGGA AGTCTTACAA CCCGCGAAGC 
TTCTGCCGGG TGTACCAGAT GGATCACCAG CCGCTAAATA CGGGAGAGCA AAAGGATATG 
GCAGAGTTCT TTATCGATTT GGTGTCCAAA CTGGAAGATA TGACGCCTGA CTTGAAGCAC 
CTGGTGAAAA GGCTCTTCTG TGGATCCTTG AGCAATAATG TCGTCTCCCT GGATTGCGGT 
CACGTTTCCC GCACGGCCGA GGACTTTTAC ACGGTTCGCT GCCAGGTTGC AGATATGCGC 
AATCTGCAGG AATCCCTGGA CGAAGTCACC GTTAAGGATA CCCTCGAGGG CGACAACATG 
TACACCTGCT CGCAGTGCGG CAAAAAAGTA CGAGCCGAGA AGCGGGCTTG CTTCAAGAAG 
CTTCCCCAGA TCTTGTGCTT CAACACCATG CGCTACACCT TCAATATGGT CACCATGCTC 
AAGGAGAAGG TCAACACGCA CTTTTCCTTT CCGCTACGCC TGAACATGTG TCACTATGTG 
GAGAAGACCT TGATGCCGCA GCAGTACAAA GAGGAGCGAG AAAGGCGCCA AAAGGAGAAG 
GAAGGAGCAG ATGGTAGTGG CGATGGGAAT GACAACGAAA AGGCAGAGGC CACGCTAGAT 
GACGATATTG AAGAATGCTA CGAGTACGAA CTGGTGGGCG TCACTGTCCA CACGGGCACA 
GCGGATGGCG GCCACTACTA CAGCTTTATA AAGGAGCGGA CGAAGACTAG TTACCATACG 
CACGAGCGGT GGTTTCTTTT CAACGATGCC GAAGTGAAGC CTTTCGATCC CTCACAGATA 
GCGGCGGAGT GCTTTGGCGG CGAGATGACG AGCAAAACCT ACGACTCAGT GACGGAGAAA 
TATCTGGACT TTAGTTTCGA GAAGACCAAC TCTGCCTACA TGCTGTTCTA CGAGCGCCGA 
CTGCCAGAGC ATCTGCAGCG CCGGCATTCC GAGCTGCTGG TTACACCCAC GCCCAGTCCC 
ACAGTGGAAG AGAAGTCCGA GGCGGAGGAG CCCACTAAAA TGGAGACAAG CTCTAGCGAA 
ATCAAGGCAG ATGTGGATGT GGAAGTGGAA GTTGAGGAGA AGGACAAGGA GAAGCCTGCG 
CAGACTGACA CAGAATCTAA GGAAACGCCT GCAAAAGAGG AGATCGCGGA TGACAAATCG 
AAGCAGGATG AGCCTGAGGA AAAGAAAATC GAAAAGCAAT CCAGAGAGGG CGAAGAGAAA 
TCGGAAACGG ACGAAAAGCC CACGGAGATG ACGACCGTGA GCACTGAGGA GGAAAAGCAG 
CCAACAGCCA ATTGTGATAA TCACCAGCAA AACAACAATA GCAACAGCAA AGCCAGCAAC 
GATCAGCAAC CATCCACGTC GAAGGCTGCC CAGAAGCTAC AACTATTTCG TCCATTGTTG 
AACAAGGAGC TCGAGGATTG GATTTGGCAG GACAATCGAC AGTTCCTGCA GGATCGCAAC 
ATTTTCGAGC ACACATATTT CAACTTTATG TGGCAGATAT GTGGGCACAT ACCCCAATCA 
TTAATATCAG AGACGGACGT CACCTGCATG GCAGCCAAGT TGTCTGTGTC CTTTTTCATT 
GAGACTTTTA TTCATGCTAA GGAAAAGCCC ACTATGGTGC CCTGGGTGGA GCTGCTGACC 
AAGCAGTTCA ATGCCAGCCA AGAGGCCTGC GAGTGGTTTC TGTCGCACAT GTCGCAGGAG 
CCCTACTGGC CGGTCCAGGT GCTAATCCAG TGTCCCAACC AAATGGTGCG TCAAATGTTT 
CAGCGTTTGG TAATCCATGT CATCCAGCAA CTACGTGCCT CACATGCTCA TCTGTATCTG 
GAAGTGGAGA CAGACGAGGA TGACAAGGAG TTAATAGGCC AAGCCTCTTG CGTCACCCGT 
TTCATTGGTT CGCTCATCTC GCTCCTGGAG CACGGTGCCC GAGCGAACCT GCGGCACTTG 
TCCGAGTACT TTGGACTGTT GTGCGAGTTC TCACGGATGG GAGATGAGGA AGCCATGTAC 
TTGCTGCGCA TCGGTGTACT CAAGAGCCTG GTCGACTTCT ATCTGGGTCA CAAGCAAACA 
GACAGCATAG ACATAAGCTC TGACAACGAA GACAACTCCT CTGAAGAGGC GCTGTCCGTG 
GAGAAGATGC GTCCGGCCTC GCTGGACAAG ATGATTGCTC TGTGCGCCAG CCTAGTGGAA 
AGATCAAGAG GAGCCGACTT CCGGCTGCGA CTCTCGCCCA AAGACTTCTC TGCAATCGCC 
GGCGGCAAGG GTTTTCCATT TTTGTACCAA CAAATAAAGG ACGGCATTAA TCCGCACCAG 
ACAAAGCATT TAATACACGC GCTTTGTCGC TGGGATGAGC GCCTGGCCAC GCAGATCATT 
GGCATGCTCT TCGCGTCGGT AACCAAGCAC ACGGAGCTGT GCGCGCCATT CTTCAAGCTG 
CTAACTCTGC TCACCGAGAC GCAGGGCGGT CCAGTGGGCC TGCCTTGTTT CACCCAGTTG 
ATCCTGCCAC GAATGTGGGA CGCCGCCGAG TACTGTCCAC AGTCGGTGCT CGACTGGCTC 
TCGCTGCAGG CCACCAAGAA CAAGATCGCC CACGCCTGGA TACTTCAATC GGCCGAGAAG 
TGGCTCGAGC AATTCCTGCT GGCCCACGAT AACACTCGTG TTCGGAACGC TGCCGCCTTT 
CTGCTGGTGG CACTGGTACC CTCCCAGCCA TTCCGGGCCA ACTTCCGCGC CCACTCGCAA 
CACAAGCTGC TGGCCCTCAA TCCGCACAGT TATCGCGACA TAAATAGCGA TGCCCAGGCG 
GTGCTGCACC AGGTGATCAC GCTGCTCCTG CGATTGCTGC GTCCAGCGCG CGTCTATGCG 
GATATCGGAG CCCATGGCAC GACCAAGCTG ACCGCGTACT TTAATCTGCT CAGCTATTGT 
ATGGTTTCAA AGACTGAAAA ACTAATGGCA AGTTCGTACA TGCGCTCCCT GTGGGAGCTG 
TTCCATCCAC GCCTCTCGGA GCCCAGTGTT CCGGCCCATC ACAATAAGCA CGCACTATTA 
ACCTTCTGGC ACCACTCACT GGTGGACTGT CCGGAGAATG CTGCCCAGGT GGCCAACTGC 
CCGGAGATTA CGCGCAACAT TGCATTTAAC TATATACTGG CCGATCATGA TGACGCGGAG 
ATAGTCACCT ACAACCGTTC CATGCTGCCC GCCTATTATG GACTACTTCG TCTGTGCTGC 
GAGCAGAGCC GTGCACTCAC ACGCCAATTG TCCCAGCATC AGAATCTTCA GTGGGCATTT 
AAAAACATCA CACCGCATCC CACGCAGTAC GCGGCCGCCG TGGACGAGCT TTTCAAGTTG 
ATGGCTCTGT TCGCCACCCG TCATCCGGAT GCTAGTGAAC AGGAAAAGCT TGATGTGACC 
CAGTTTAGGA GGGCTGTGAT TGTGTCCTAC ACCAGTAGTC TGGATGCACG TGTCTCTTGG 
TCCACGCTGA TCAGTGCTCT TAAGATACTC GTGGACAATG AGGAGGACCG CACGATGGTG 
ATCTTCAACG GCGGCATTGA GATGTGTTTC GAGGCGCTGC ACACGCTACA TTCCATGCAC 
CACGAGGCCA CCGCCTGCCA CGTGGCCGGA GACCTATTCG ATCTCTTGGG CGAGATGCTA 
CTCCTCCTAG CCACTCTTCG AACCCGCACA GACAGTCCTG CTCAAAAGAA GCAGCAGCAA 
CAACAGCAGC AGCTGGAGCA ACAGTTGCAG TTGCAGCAGC AACAGATGCT GCAGAAACAA 
CAGCAGCAGT CGCAAAGCCA AACGCAAACA CCGCAGTCGC CGCAGCAAAA GGAAAAGCAG 
CTGCAGCAAC AAATGCAGCA GCATCTGCAG CTGCAACAGT TGCAGCAAAT GCAGTTCCAG 
CAGCAACACT TCCTGCGCCA GCAACATCAA CACTCGGCGC TGGCAAAGGC ACTGCCGGAT 
GCAGTGAAGC GACTGGCTAC GCTACTCAAC ACCTTCAATT CGCCAGAGAT CAGTCGCATG 
GCCTTGGAGG TGCTCAAGGA GCTGGTGCGC AATCCCAGCC TGGAAACCAT TAGTATTCTG 
GCGCCCATTC TTATCAACTG TCATCTGTCC GTGGCGAATG CTCCCAATGC GATCGGTCCT 
CTGGGACCGT ACTTTCCGCG TCGCGGTGCG AAGCATACGC CTTGGCCATT GGGAGCCAAG 
AACTCACCCC GTCCACCGCG GCCCATGGTA CAGATGTGCA TCGCTCTGGC TGAGCTGACA 
CCACGCGGTC TCGATGCCGA TTACGACGTT CAGGTGGAGT CCTTTTACCG ACCCTACCAT 
GACTTTATCG ATGTCATGAT GCGCATGTGC GTCAACACGG GCACTCTGAA CGACACGCTA 
GTCAAGCTGC AATGCTTGGT GGCCATCGAG TCCACGCCGC TGCACTTCAC CTATTTCCCC 
AAATTCTGGG TGGGCATACA CAACAATGCT CTGACGCACA AATACGTTGA GCTGCTGGTG 
AAAAATCAAC TGTTGGTAGA GTACCTTCAC AATGTCCTTA GGGACGAGCG AAGCATGTTG 
AAGGATGCCT GCGTTCGTGA ATTTCTGGAG CTCTATTATC ACAAGGTTGC CGCCCAGCTG 
CCAGTGGCCC GTATGATCTA CACAATCAAC TATGGTATGC ACTCCAAGGA CGACATTGAT 
GAGCTGTGTG GCGATCTGTT CGCCATCCGA ATCATTGCCC AGGCCACTGG AGTGCCGGCC 
TCAGTGCGCA AGGAGTTGCG AGGATCGCTG AGGGCGCTTC AGAACAAGAG CGAGCGGTTT 
AGGAAGGAAT CGGAGAGGGA TCCATTTCCG AACAAGAAGC AGAAACGTGA CTCGCAAAAG 
ATCAAGGAAA AGGAGCACCC TCAGCCGGAG AGCGAGAAAG AGACCTCAAC GGAGAACGAC 
AAACCATCAG ATGTTTCCAT GGAATCCAGC GGTAACGCGG AGCAAGCAAC GGACAGCACA 
AAACCACCAA CTCCTGCAGG CAGCGACGAT GAGCAGATGG ACAAAACTTC AGTTCCCTCG 
TCCGATGACG AGACAGAGTT GGAGGACGAG CTGCAGCCGA CGCCCAAGCG CAACAAGAAG 
GCTTCGAATA AGAAGACCGC CCAGGATCGT GTCAACGAGG AGCGCGAGAA GCGACTTATC 
ACGCTGATCA CCATGGAATC ATATATCCAG AGCATTTTTG CCATCCTTAA GAGAGATGCC 
AGTGTCCCCA GTTCGGGAGC GACTAAGGAG CCCAGTGAGG AACCGTGTGG CGAGGCATCC 
ACTTCGGCGG CTGCCGCTGT AAAGTTATCT CGTCCCAAAG GAGCCTGTAC TCCGCCGGAG 
CCAATTACCG ACGTAAACGA TACGCGTTGC AACATAGATA CTGAGACAGA GGCGGAGGCG 
GATGAGCAAT CTCCAAAGAC GAGCCAGACA AATGGTAGCC AACAGAACGA ATCCCCGCCA 
GCGGCGACCA GTGCAGACAC TGCACCCGCG AATCCTTCAC CAGCTCCCGC GGCCGCCGTT 
GCGTCCACAT CCCAGGCGGC AAGTCCAACA CAGATTTAGT TGTCATTCAA CGATTTGCTC 
AGCATTTTGT TTTTCGGCAA GCCATTGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

puf ( NM_001275993.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262922.1 Drosophila melanogaster puffyeye (puf), transcript variant G, mRNA.

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CloneID	OFa07739
Clone ID Related Accession (Same CDS sequence)	NM_001275993.2
Accession Version	NM_001275993.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	puffyeye, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001275993.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCGAGG TGTGCGCCGA CTTCCAGAAT CTCCTGGAGC TCTATGAGGT GCGCGTGGCC 
AGCTCGGATC TGAAGTTCCA GCTGCTGCTG AAGAGCGAGA TCGAGACCAC CTTCAACTAC 
ATCCAGTCGT GGCCGCAGCG TCAGTGCATG TGCCTGTACC GGGACACCAA GAACTATGAC 
CGTTTCAACC TGGTAGTGCA GTCGCTCATC TGTCTGACGG TGCAGCATCT GAAGCACATC 
GACCACCTCA TCGACAACTA CAAGCGGCTA ACGGCCAGTG CTGCCCAGGT GGTGGCCCAG 
GCCCAGCAGC AGCGTGAACA GCAGCGGGAC GAAGCGTCAG CCCAGGCAGA GGCAAAGGAA 
TCATCCGCGC CAGCGGAAGA ACCTAAGAAG GAGGAGCCAT CTGGGTCCGC CGGTGAGGAG 
GCTCAGGGCT CCGGCGACGG GCCAGCCAAA AAGCCGCCAG TTGGCCCCTG CACTCCCCCT 
CCCCCGCAAA CGGCCAATCC TCAGCACAAA TCCCACCTGC AGTACACAGA GGAGCCCTGG 
ATCCTGCCCG AGGTGGAGAA ACTGTTGGTG CTCGTTTCAA AGGTGTTCCT GCTCAACTTC 
CCGCTGTACA TCGCCCACAA ACATGGCATG CACTCTCGGC TGGACGACCT GCAGGCTGAG 
GAGGCCCACC ACCTGGCCCT CATCTGCGAT CTTCACGACA ACGACCTGCC CATCTATCTG 
CTGCGGAATG TGAGCCTGTT CTGCAACTCG GGCGGCTTTG GCGCCATGTC GCTGTGCTTC 
GAACATCCCG ATCTGCCAGT GTCCACCGCT CACTCGATGA CCGCTGCCGT CTCAAATGTG 
AAACTTTGGC TGAACTATCA CTGTAATACT CAGCTCTTCG TGCCGCTGCG AAGTCGGATA 
CTGCAGTACA TGTGCAAGCT GTCGGATCAG AGCCTCCGAT CTGCCGCAAC CCGCGCGATG 
GCCGATTTTG TGTGGTCCTC TATGCGAGAC CCACTGGATG TTGCTGTCAA CTTCGACACC 
GAAGGATTGG CCCTGGCCTT TAAGTACTTC ACATCGACCA CGCTAACCAT GCGACTGGCA 
GGCATGGCCC AGATCAACGC ACACATTAAT CTATTTAACG AGATCTGCAC CACCGAAACG 
GTCAACGAAG TGGAGCTGTT CGGGCAGCGC ATCGCGAACT GGCTGACTGA AAATCATATT 
GTACAGCACC TGTTTGGCCC GAATCTGCAC GTAGAAATCG TTAAACAGGC ACACGTGCTG 
CTCAACTTTT TGGCGGTAGA GAACCAGATC TCTGAAGAGG ACATCAAGCT GATCTGGCAA 
GCCACCCAGC TGAAGCACTG CTCAAAGACG ATCTTCGACA TCCTGCCTTC GCTGGTGAAG 
AATCTGACCC CGCGCCCGGC AATGCACCTT TACTCCCTCC TTTGCCGCAT GGATCCCAAG 
GAGCACACTG AGCAGAGCAT ATACATAGCC TCAGCGCTAA CAAAACAGCT GTGGACTCGT 
GATACCTCAC GCAGTCAGAT GAACCTGATG CAAGACCACC TGCTGGGGTC CAACGTCACC 
GCTTCCAGCT CTGACAGCGG CAGCATTGAG GGCAGCAACA CAGAGGACGA CCACGTGGGC 
GCCGACGACA GTAGCATTGC CAGCGGTGGC GGCGGCGGAG GTGTTGGTAA CAAGAGTCCA 
ATCGATGGAG TTACTCCATG CAAGCAGGCC CGGCACAGGC GGCACATCTG TGACCCCACC 
ACCGAGAAGG GAAAGCAAAT AAGTCCCGAG GATATGGCCA AAGTAGACTT GGTGAACAAA 
CGGATTGTGA ACATTATCGA CAACACAAGC AGTGAAGAGG AGCTGCAATC GCGGGCCGCA 
CTGGAGCTGC AACTGCATCG CAGCCGCAAA AAGACGAGCA ACAAGCGACG GCGACAAAAA 
ACCAACAAAC AGATCATTCT GCCCCATGAG CTGGTCGAGA TTTGGGACGG AGTTGAAGAT 
GTGCCCAGCA GTGACGAGGC TGATGGCGAA GCCGATGGTG ATGGCGAGGG CGAACTTTTA 
GCGGACAGCG ATGAGTGCAG CGATGGTGCT ACGAGTCAGC TGGTACCCGA CGCCGTGCTA 
AAGCATCTGC AGGGAGAGGG ACCTTTTATC AGGGCCATCG AGACCAATAT CAATGAGCTG 
CTCAGTGGTG CCGAGAACGA TGGCAGTTAC TCATCCCCGA TGAGTAACAA ATCTGAAAAG 
AACCTGGCTG ACTTCGATGA CGAGGACGTG TCGCCTTGCG AGGAGGAGCT GGCCCAGCTG 
GTCAGCTCCC GCGCCAATTG TTCGGACGTT CCACCAGCCT TTGCAGCCGC TGCCGCCGCC 
ATGATGGTGG CCCAGTCGGC GATGGCCCTT CAGAAGTCTG GAGAGTCGAA TGTGGCTGCT 
GCTGCCGCTG CTGTGGCCGC TGCTGCAGCG GCTACCGGAA CTGCACAGCA AACGCTGGTG 
GCAATGAACC GGCAGGCCAG CGTTGCTGCA GCTGCCGCCG TGGTTGCTGC GGCCAAGGCA 
AAATCGGACT CAGATGTCGA TCTGATGGAT GTGGTTTCCG GCGGAAAGCA GCACCATTCA 
CCAAAGGCGA GCCAGGGCAG CTCTACGTCC GGGAGTACAC CGGTGCAGCC CTCGTTCAAG 
CTAAATGATG TCTGCCAGCC CGGAAACACA CTGCTTTGGG ATCTGCTTCA AGACGATAAA 
ATTGGCCAAC TAGGCGAGTC TCTGGCTCTA GAGGCGGAGA AAGCTCTGGC CACTCTCCTG 
TGCTTCAGCA TGGACCGCCA ACTGCGAACG AAATTCATCG AGGGTTGTCT GTATAACGTC 
GCCAACAACC GGAGTGTTAT TGTCAGTCTG CGACTGCTGC CCAAACTGTT CGCCTCGTTC 
CAACAGTTTC GACCCTCGGA CACGCACTCT ATGACGATGT GGGCAGAGCG AAATCACCGG 
ATGATGCAGT GCTTTTTCAA TAATATACGT CACTATGCGC GTCGCCATGC CGAGGTACTG 
ATCACCCAAA ACGGAGAGCA GCAGCAGCAG CAGCTGGGCG GACAACTCTA CTCGCACAAG 
ACACAAGTTT CTGTAAGACT GCAGTTCCTA TCATCGATAT TCTCGACGGT GGGATCCCCG 
AAAAGCTTCC GCCTAACGCT GGAGCAGCTT GACGCGCTAT GGGAGTGGCT GGCCCACGAT 
CCGGAGTGTG CCGACTGCTA CTTTTCGTGG CTGCAGGCCC AGGCCAAAGG CGGTGATCAG 
CATGCTCTGG GCATTGAAGC ACTTCAGCAT TTGTATTTGA AGAAACTCCC AGAGCTGCGA 
CCCGAGGAGT TCTCTATGGT TGCGTTGGGA CTATTTCAAC AGCTCTGTAG CTTCGCCCGC 
ATCGCCATGG CCGAGTACGA CAATCATAGC GACCAGATCT CGGCGAGCGC CAGTGCCGTT 
GGCATGTACC ATCTTTGGAA GATAGCGTTG CGTGCCCAGA GCAACGATGT CTCCTTGGCG 
GCCATACAGT ACATCAACAT GTACTACATG GGTCAACAAT TGCGCCTGGA GAAGGAGTTC 
GTTTCACAGT GTATGGAAAA CCTAGTTCAA GCGGCCACGG CTTTGGAAAG CATCGATGAT 
GAGAATGCTT TGATGCGTGT CCAGCGTGGC CTGCTCTTGC TGAACACCCA TTTGGATACG 
TTCCGGCGTC GATATGCGTT TCATCTGCGC CGCTGGGCCA TCGAAGGCAA AGGGATTGGA 
TCGCACAGTA ACCTTAAAAA TGAGGGAGCG GGTCCACCAC TACGAATAGT GCTGCAACAG 
GCAGGACTTT CGGAAAAAAG CCTTTTGCAG ATGCACGCGT GTGACTTAAT CGCCGACCTC 
AAGGCTGAGG TGTCCAAGTG GTGGGAGAGC CTTCAAACTG GACTAGCTGC TCCTGTGCTC 
GGTCTGCTGC TCAGCGATGG ACCGCTACGC ATTATCACAC AGGGACAGGA ATTGACCTCG 
GATTACGATG AACGGAGTCT CGGAGATGCT GGTTTCAAGG ATAACCAGAT CGTCTACGTA 
TCGTTAGGTG GACGTGGAGC CAGGCGAAAG GAATCCAATT TGGAGCATCC GTCAATGCTG 
CCGCCCCCAC CAAAGGAGTG TTTGCCCACG GTGCTCCTGC TGCAGCCAAA GTACTTCGAG 
AAGCTATTTT GCCTGATGCA GACCCTGGGC GATATGCAAC CGCAAGCGTC GACTGTAAAT 
CCCCAGCACC ACACAAAGGC GCAATTGCTC TCGCGACGCG TATGGGATAT CCTCGCCATG 
CTGCCAACTA ATCCGCACAT TCTAGACGCC TTTAAGAGTC TGGTTACGGA CCTAAGCGAA 
CTCGAGCAGC TTGATGCTGG CGGAGAGGAG GAGCAACTGG CCACCAAGCG CAAGCAAATC 
AAGCAGAAGT TCCGAGATCT TCTCGACCCG AACAACTTGC AAAAGTTCAT GTACTCCCTC 
CATATTGTGG AGAGTCTGGC CCTAACAAGT TCCAGACGTG GTGAATCCAA CGGCAATGTG 
GCCATGGGAA ATACACCGGA ACAGGTACGG GTGAAGAAAT CCTCGATGGG AAGACGGAGA 
AATAGCAACG AGCAGCCGCC ACCGCCGCCG CCAGAAGTCA AGATGAGCAA GGAGCAACTG 
TGCGAGCTGG AGGCCCCACT GACGCCCACA CCCTCGACTG GCTTGCAGGA TGTGGAAACG 
GAGGCCAGCA GTAGCAGCGG TGGCGACAAA GAAAACCAAC CGAAACAACA CAGCAAGCGT 
CAGAAGAAAG GCGAAACTTT TGAACAGGAA AAGGAGCGCC CAGTGGGTTG CTCCACGCCG 
CCGTCTCCAA CTCCTCCGCC ACCAGCACTT TCGGTAGTGG AAAGAGGTGA CAATAAGTGG 
AGTGAGGCGT TCGTCAAATG TGGTGGCCTT CGTCATCTGT ACGAAATCTT TAGTGAGGGC 
CAACTACAAC AAAGCGCACA TCCCAAGGAG TTGGCTTTAA ATGAATGGCG ACACGATTGC 
CTGGCCAGCC TGCTGCGCAT TCTCTGGCTT CTTGGATTCG AGGAACTGCA GTCGGCGGAT 
GCCCATGTTC TAATGTCACG TCCGCACCCG TTTATGCTAC AGTTGATGGA GGTACCACAG 
TGCCTGACCC GACTCTCATC GATCCTTAAC GACGAAGTTC ACCAGCAGCA TCAGGCTTCT 
TCGGCCAATC CACTGGTATT CCCCTATCAG TTCCAGCACT TGCGTACTGG CTTTTGGGGC 
AGGGCCCAGC TCATCCAGTT CGCTATGAAC ATTCTAGTGA GTTTTGTGCA CGCCTCAGCG 
GAAGCGAGAA GGCTTCTCTG GGCCCCAACT GGTACCGATC ACTGTCGATG GCTGCAGAAG 
TTCATCCTGG AGGATCCCGA ACCGGCGGTA CGGCGTGAGA TATGCGCTGG TCTTTATCGC 
ATTTGCTTAG GAAACGCACA TAGCTATCGC CTCTTACTAG CACCACTGCT GCACAAGCTC 
ATCGCCCTGT TGCCATTGGC CGAGCAGATG AGCTCTGGCA ACCAGCACAC GCAGTTTTTG 
CTCTCGGAAG AGGGCAAGGA TCCCTACGGA CCCGCCTGCC GTGACTACTT TTGGCTGCTG 
GCCAGATTGG TGGATACTCT CTCCCCGGAA ATGGTGGCCG AGGAGCACAT CGACATTGAA 
ATGCTGTGCG AAAGCATCTC GCAGAGCATT CTCACCCGAG AGTATTATGA ACTGCGCCAT 
GGTTACCAGG ACGATGGCTT GGTGGGTCTC CTAAATCTCA TGTCCAATTT GATCAAGTAC 
GACACCACAT TCAAGTACAC ACCCAAGGCG TTATCCTTTA TTGAGCAACT GATTGGCTTC 
CTGTTTGACA TGCCCTCGCC GGCAGATCGC CAGAAGCCCA AGTGCAAGTC GGCGTCGTCT 
CGAGCATCTG CCTACGATCT GCTGGTTGAA CTTTGCCGTG GATGCGCCAC AAACTATGCA 
TATCTGCATG GACGTCTGTT GGCACAGCAC AAATCCGGAC CCAAGCAGCC GTATCCCTGG 
GACTATTGGC CGCGCGATGA AGGCAGAGCT GAGTGCGGAT ATGTGGGGCT AACTAATTTG 
GGAGCCACTT GCTATATGGC CTCTTGCGTC CAGCACTTGT ACATGATGCC ACAGGCGCGA 
GCAGCTGTTT TGCGAGTTCC ACCCAACGCC GCCCGTAAGC ATGGGCCCAC ACTTCTGGAA 
CTACAACGGA TGTTTGCCTA CCTACTGGAG TCGGAACGGA AGTCTTACAA CCCGCGAAGC 
TTCTGCCGGG TGTACCAGAT GGATCACCAG CCGCTAAATA CGGGAGAGCA AAAGGATATG 
GCAGAGTTCT TTATCGATTT GGTGTCCAAA CTGGAAGATA TGACGCCTGA CTTGAAGCAC 
CTGGTGAAAA GGCTCTTCTG TGGATCCTTG AGCAATAATG TCGTCTCCCT GGATTGCGGT 
CACGTTTCCC GCACGGCCGA GGACTTTTAC ACGGTTCGCT GCCAGGTTGC AGATATGCGC 
AATCTGCAGG AATCCCTGGA CGAAGTCACC GTTAAGGATA CCCTCGAGGG CGACAACATG 
TACACCTGCT CGCAGTGCGG CAAAAAAGTA CGAGCCGAGA AGCGGGCTTG CTTCAAGAAG 
CTTCCCCAGA TCTTGTGCTT CAACACCATG CGCTACACCT TCAATATGGT CACCATGCTC 
AAGGAGAAGG TCAACACGCA CTTTTCCTTT CCGCTACGCC TGAACATGTG TCACTATGTG 
GAGAAGACCT TGATGCCGCA GCAGTACAAA GAGGAGCGAG AAAGGCGCCA AAAGGAGAAG 
GAAGGAGCAG ATGGTAGTGG CGATGGGAAT GACAACGAAA AGGCAGAGGC CACGCTAGAT 
GACGATATTG AAGAATGCTA CGAGTACGAA CTGGTGGGCG TCACTGTCCA CACGGGCACA 
GCGGATGGCG GCCACTACTA CAGCTTTATA AAGGAGCGGA CGAAGACTAG TTACCATACG 
CACGAGCGGT GGTTTCTTTT CAACGATGCC GAAGTGAAGC CTTTCGATCC CTCACAGATA 
GCGGCGGAGT GCTTTGGCGG CGAGATGACG AGCAAAACCT ACGACTCAGT GACGGAGAAA 
TATCTGGACT TTAGTTTCGA GAAGACCAAC TCTGCCTACA TGCTGTTCTA CGAGCGCCGA 
CTGCCAGAGC ATCTGCAGCG CCGGCATTCC GAGCTGCTGG TTACACCCAC GCCCAGTCCC 
ACAGTGGAAG AGAAGTCCGA GGCGGAGGAG CCCACTAAAA TGGAGACAAG CTCTAGCGAA 
ATCAAGGCAG ATGTGGATGT GGAAGTGGAA GTTGAGGAGA AGGACAAGGA GAAGCCTGCG 
CAGACTGACA CAGAATCTAA GGAAACGCCT GCAAAAGAGG AGATCGCGGA TGACAAATCG 
AAGCAGGATG AGCCTGAGGA AAAGAAAATC GAAAAGCAAT CCAGAGAGGG CGAAGAGAAA 
TCGGAAACGG ACGAAAAGCC CACGGAGATG ACGACCGTGA GCACTGAGGA GGAAAAGCAG 
CCAACAGCCA ATTGTGATAA TCACCAGCAA AACAACAATA GCAACAGCAA AGCCAGCAAC 
GATCAGCAAC CATCCACGTC GAAGGCTGCC CAGAAGCTAC AACTATTTCG TCCATTGTTG 
AACAAGGAGC TCGAGGATTG GATTTGGCAG GACAATCGAC AGTTCCTGCA GGATCGCAAC 
ATTTTCGAGC ACACATATTT CAACTTTATG TGGCAGATAT GTGGGCACAT ACCCCAATCA 
TTAATATCAG AGACGGACGT CACCTGCATG GCAGCCAAGT TGTCTGTGTC CTTTTTCATT 
GAGACTTTTA TTCATGCTAA GGAAAAGCCC ACTATGGTGC CCTGGGTGGA GCTGCTGACC 
AAGCAGTTCA ATGCCAGCCA AGAGGCCTGC GAGTGGTTTC TGTCGCACAT GTCGCAGGAG 
CCCTACTGGC CGGTCCAGGT GCTAATCCAG TGTCCCAACC AAATGGTGCG TCAAATGTTT 
CAGCGTTTGG TAATCCATGT CATCCAGCAA CTACGTGCCT CACATGCTCA TCTGTATCTG 
GAAGTGGAGA CAGACGAGGA TGACAAGGAG TTAATAGGCC AAGCCTCTTG CGTCACCCGT 
TTCATTGGTT CGCTCATCTC GCTCCTGGAG CACGGTGCCC GAGCGAACCT GCGGCACTTG 
TCCGAGTACT TTGGACTGTT GTGCGAGTTC TCACGGATGG GAGATGAGGA AGCCATGTAC 
TTGCTGCGCA TCGGTGTACT CAAGAGCCTG GTCGACTTCT ATCTGGGTCA CAAGCAAACA 
GACAGCATAG ACATAAGCTC TGACAACGAA GACAACTCCT CTGAAGAGGC GCTGTCCGTG 
GAGAAGATGC GTCCGGCCTC GCTGGACAAG ATGATTGCTC TGTGCGCCAG CCTAGTGGAA 
AGATCAAGAG GAGCCGACTT CCGGCTGCGA CTCTCGCCCA AAGACTTCTC TGCAATCGCC 
GGCGGCAAGG GTTTTCCATT TTTGTACCAA CAAATAAAGG ACGGCATTAA TCCGCACCAG 
ACAAAGCATT TAATACACGC GCTTTGTCGC TGGGATGAGC GCCTGGCCAC GCAGATCATT 
GGCATGCTCT TCGCGTCGGT AACCAAGCAC ACGGAGCTGT GCGCGCCATT CTTCAAGCTG 
CTAACTCTGC TCACCGAGAC GCAGGGCGGT CCAGTGGGCC TGCCTTGTTT CACCCAGTTG 
ATCCTGCCAC GAATGTGGGA CGCCGCCGAG TACTGTCCAC AGTCGGTGCT CGACTGGCTC 
TCGCTGCAGG CCACCAAGAA CAAGATCGCC CACGCCTGGA TACTTCAATC GGCCGAGAAG 
TGGCTCGAGC AATTCCTGCT GGCCCACGAT AACACTCGTG TTCGGAACGC TGCCGCCTTT 
CTGCTGGTGG CACTGGTACC CTCCCAGCCA TTCCGGGCCA ACTTCCGCGC CCACTCGCAA 
CACAAGCTGC TGGCCCTCAA TCCGCACAGT TATCGCGACA TAAATAGCGA TGCCCAGGCG 
GTGCTGCACC AGGTGATCAC GCTGCTCCTG CGATTGCTGC GTCCAGCGCG CGTCTATGCG 
GATATCGGAG CCCATGGCAC GACCAAGCTG ACCGCGTACT TTAATCTGCT CAGCTATTGT 
ATGGTTTCAA AGACTGAAAA ACTAATGGCA AGTTCGTACA TGCGCTCCCT GTGGGAGCTG 
TTCCATCCAC GCCTCTCGGA GCCCAGTGTT CCGGCCCATC ACAATAAGCA CGCACTATTA 
ACCTTCTGGC ACCACTCACT GGTGGACTGT CCGGAGAATG CTGCCCAGGT GGCCAACTGC 
CCGGAGATTA CGCGCAACAT TGCATTTAAC TATATACTGG CCGATCATGA TGACGCGGAG 
ATAGTCACCT ACAACCGTTC CATGCTGCCC GCCTATTATG GACTACTTCG TCTGTGCTGC 
GAGCAGAGCC GTGCACTCAC ACGCCAATTG TCCCAGCATC AGAATCTTCA GTGGGCATTT 
AAAAACATCA CACCGCATCC CACGCAGTAC GCGGCCGCCG TGGACGAGCT TTTCAAGTTG 
ATGGCTCTGT TCGCCACCCG TCATCCGGAT GCTAGTGAAC AGGAAAAGCT TGATGTGACC 
CAGTTTAGGA GGGCTGTGAT TGTGTCCTAC ACCAGTAGTC TGGATGCACG TGTCTCTTGG 
TCCACGCTGA TCAGTGCTCT TAAGATACTC GTGGACAATG AGGAGGACCG CACGATGGTG 
ATCTTCAACG GCGGCATTGA GATGTGTTTC GAGGCGCTGC ACACGCTACA TTCCATGCAC 
CACGAGGCCA CCGCCTGCCA CGTGGCCGGA GACCTATTCG ATCTCTTGGG CGAGATGCTA 
CTCCTCCTAG CCACTCTTCG AACCCGCACA GACAGTCCTG CTCAAAAGAA GCAGCAGCAA 
CAACAGCAGC AGCTGGAGCA ACAGTTGCAG TTGCAGCAGC AACAGATGCT GCAGAAACAA 
CAGCAGCAGT CGCAAAGCCA AACGCAAACA CCGCAGTCGC CGCAGCAAAA GGAAAAGCAG 
CTGCAGCAAC AAATGCAGCA GCATCTGCAG CTGCAACAGT TGCAGCAAAT GCAGTTCCAG 
CAGCAACACT TCCTGCGCCA GCAACATCAA CACTCGGCGC TGGCAAAGGC ACTGCCGGAT 
GCAGTGAAGC GACTGGCTAC GCTACTCAAC ACCTTCAATT CGCCAGAGAT CAGTCGCATG 
GCCTTGGAGG TGCTCAAGGA GCTGGTGCGC AATCCCAGCC TGGAAACCAT TAGTATTCTG 
GCGCCCATTC TTATCAACTG TCATCTGTCC GTGGCGAATG CTCCCAATGC GATCGGTCCT 
CTGGGACCGT ACTTTCCGCG TCGCGGTGCG AAGCATACGC CTTGGCCATT GGGAGCCAAG 
AACTCACCCC GTCCACCGCG GCCCATGGTA CAGATGTGCA TCGCTCTGGC TGAGCTGACA 
CCACGCGGTC TCGATGCCGA TTACGACGTT CAGGTGGAGT CCTTTTACCG ACCCTACCAT 
GACTTTATCG ATGTCATGAT GCGCATGTGC GTCAACACGG GCACTCTGAA CGACACGCTA 
GTCAAGCTGC AATGCTTGGT GGCCATCGAG TCCACGCCGC TGCACTTCAC CTATTTCCCC 
AAATTCTGGG TGGGCATACA CAACAATGCT CTGACGCACA AATACGTTGA GCTGCTGGTG 
AAAAATCAAC TGTTGGTAGA GTACCTTCAC AATGTCCTTA GGGACGAGCG AAGCATGTTG 
AAGGATGCCT GCGTTCGTGA ATTTCTGGAG CTCTATTATC ACAAGGTTGC CGCCCAGCTG 
CCAGTGGCCC GTATGATCTA CACAATCAAC TATGGTATGC ACTCCAAGGA CGACATTGAT 
GAGCTGTGTG GCGATCTGTT CGCCATCCGA ATCATTGCCC AGGCCACTGG AGTGCCGGCC 
TCAGTGCGCA AGGAGTTGCG AGGATCGCTG AGGGCGCTTC AGAACAAGAG CGAGCGGTTT 
AGGAAGGAAT CGGAGAGGGA GCTATCATCA TCTCCCTATT TGCCAGATCC ATTTCCGAAC 
AAGAAGCAGA AACGTGACTC GCAAAAGATC AAGGAAAAGG AGCACCCTCA GCCGGAGAGC 
GAGAAAGAGA CCTCAACGGA GAACGACAAA CCATCAGATG TTTCCATGGA ATCCAGCGGT 
AACGCGGAGC AAGCAACGGA CAGCACAAAA CCACCAACTC CTGCAGGCAG CGACGATGAG 
CAGATGGACA AAACTTCAGT TCCCTCGTCC GATGACGAGA CAGAGTTGGA GGACGAGCTG 
CAGCCGACGC CCAAGCGCAA CAAGAAGGCT TCGAATAAGA AGACCGCCCA GGATCGTGTC 
AACGAGGAGC GCGAGAAGCG ACTTATCACG CTGATCACCA TGGAATCATA TATCCAGAGC 
ATTTTTGCCA TCCTTAAGAG AGATGCCAGT GTCCCCAGTT CGGGAGCGAC TAAGGAGCCC 
AGTGAGGAAC CGTGTGGCGA GGCATCCACT TCGGCGGCTG CCGCTGTAAA GTTATCTCGT 
CCCAAAGGAG CCTGTACTCC GCCGGAGCCA ATTACCGACG TAAACGATAC GCGTTGCAAC 
ATAGATACTG AGACAGAGGC GGAGGCGGAT GAGCAATCTC CAAAGACGAG CCAGACAAAT 
GGTAGCCAAC AGAACGAATC CCCGCCAGCG GCGACCAGTG CAGACACTGC ACCCGCGAAT 
CCTTCACCAG CTCCCGCGGC CGCCGTTGCG TCCACATCCC AGGCGGCAAG TCCAACACAG 
ATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262922.1
CDS	279..12044
Translation	MCEVCADFQNLLELYEVRVASSDLKFQLLLKSEIETTFNYIQSWPQRQCMCLYRDTKNYDRFNLVVQSLICLTVQHLKHIDHLIDNYKRLTASAAQVVAQAQQQREQQRDEASAQAEAKESSAPAEEPKKEEPSGSAGEEAQGSGDGPAKKPPVGPCTPPPPQTANPQHKSHLQYTEEPWILPEVEKLLVLVSKVFLLNFPLYIAHKHGMHSRLDDLQAEEAHHLALICDLHDNDLPIYLLRNVSLFCNSGGFGAMSLCFEHPDLPVSTAHSMTAAVSNVKLWLNYHCNTQLFVPLRSRILQYMCKLSDQSLRSAATRAMADFVWSSMRDPLDVAVNFDTEGLALAFKYFTSTTLTMRLAGMAQINAHINLFNEICTTETVNEVELFGQRIANWLTENHIVQHLFGPNLHVEIVKQAHVLLNFLAVENQISEEDIKLIWQATQLKHCSKTIFDILPSLVKNLTPRPAMHLYSLLCRMDPKEHTEQSIYIASALTKQLWTRDTSRSQMNLMQDHLLGSNVTASSSDSGSIEGSNTEDDHVGADDSSIASGGGGGGVGNKSPIDGVTPCKQARHRRHICDPTTEKGKQISPEDMAKVDLVNKRIVNIIDNTSSEEELQSRAALELQLHRSRKKTSNKRRRQKTNKQIILPHELVEIWDGVEDVPSSDEADGEADGDGEGELLADSDECSDGATSQLVPDAVLKHLQGEGPFIRAIETNINELLSGAENDGSYSSPMSNKSEKNLADFDDEDVSPCEEELAQLVSSRANCSDVPPAFAAAAAAMMVAQSAMALQKSGESNVAAAAAAVAAAAAATGTAQQTLVAMNRQASVAAAAAVVAAAKAKSDSDVDLMDVVSGGKQHHSPKASQGSSTSGSTPVQPSFKLNDVCQPGNTLLWDLLQDDKIGQLGESLALEAEKALATLLCFSMDRQLRTKFIEGCLYNVANNRSVIVSLRLLPKLFASFQQFRPSDTHSMTMWAERNHRMMQCFFNNIRHYARRHAEVLITQNGEQQQQQLGGQLYSHKTQVSVRLQFLSSIFSTVGSPKSFRLTLEQLDALWEWLAHDPECADCYFSWLQAQAKGGDQHALGIEALQHLYLKKLPELRPEEFSMVALGLFQQLCSFARIAMAEYDNHSDQISASASAVGMYHLWKIALRAQSNDVSLAAIQYINMYYMGQQLRLEKEFVSQCMENLVQAATALESIDDENALMRVQRGLLLLNTHLDTFRRRYAFHLRRWAIEGKGIGSHSNLKNEGAGPPLRIVLQQAGLSEKSLLQMHACDLIADLKAEVSKWWESLQTGLAAPVLGLLLSDGPLRIITQGQELTSDYDERSLGDAGFKDNQIVYVSLGGRGARRKESNLEHPSMLPPPPKECLPTVLLLQPKYFEKLFCLMQTLGDMQPQASTVNPQHHTKAQLLSRRVWDILAMLPTNPHILDAFKSLVTDLSELEQLDAGGEEEQLATKRKQIKQKFRDLLDPNNLQKFMYSLHIVESLALTSSRRGESNGNVAMGNTPEQVRVKKSSMGRRRNSNEQPPPPPPEVKMSKEQLCELEAPLTPTPSTGLQDVETEASSSSGGDKENQPKQHSKRQKKGETFEQEKERPVGCSTPPSPTPPPPALSVVERGDNKWSEAFVKCGGLRHLYEIFSEGQLQQSAHPKELALNEWRHDCLASLLRILWLLGFEELQSADAHVLMSRPHPFMLQLMEVPQCLTRLSSILNDEVHQQHQASSANPLVFPYQFQHLRTGFWGRAQLIQFAMNILVSFVHASAEARRLLWAPTGTDHCRWLQKFILEDPEPAVRREICAGLYRICLGNAHSYRLLLAPLLHKLIALLPLAEQMSSGNQHTQFLLSEEGKDPYGPACRDYFWLLARLVDTLSPEMVAEEHIDIEMLCESISQSILTREYYELRHGYQDDGLVGLLNLMSNLIKYDTTFKYTPKALSFIEQLIGFLFDMPSPADRQKPKCKSASSRASAYDLLVELCRGCATNYAYLHGRLLAQHKSGPKQPYPWDYWPRDEGRAECGYVGLTNLGATCYMASCVQHLYMMPQARAAVLRVPPNAARKHGPTLLELQRMFAYLLESERKSYNPRSFCRVYQMDHQPLNTGEQKDMAEFFIDLVSKLEDMTPDLKHLVKRLFCGSLSNNVVSLDCGHVSRTAEDFYTVRCQVADMRNLQESLDEVTVKDTLEGDNMYTCSQCGKKVRAEKRACFKKLPQILCFNTMRYTFNMVTMLKEKVNTHFSFPLRLNMCHYVEKTLMPQQYKEERERRQKEKEGADGSGDGNDNEKAEATLDDDIEECYEYELVGVTVHTGTADGGHYYSFIKERTKTSYHTHERWFLFNDAEVKPFDPSQIAAECFGGEMTSKTYDSVTEKYLDFSFEKTNSAYMLFYERRLPEHLQRRHSELLVTPTPSPTVEEKSEAEEPTKMETSSSEIKADVDVEVEVEEKDKEKPAQTDTESKETPAKEEIADDKSKQDEPEEKKIEKQSREGEEKSETDEKPTEMTTVSTEEEKQPTANCDNHQQNNNSNSKASNDQQPSTSKAAQKLQLFRPLLNKELEDWIWQDNRQFLQDRNIFEHTYFNFMWQICGHIPQSLISETDVTCMAAKLSVSFFIETFIHAKEKPTMVPWVELLTKQFNASQEACEWFLSHMSQEPYWPVQVLIQCPNQMVRQMFQRLVIHVIQQLRASHAHLYLEVETDEDDKELIGQASCVTRFIGSLISLLEHGARANLRHLSEYFGLLCEFSRMGDEEAMYLLRIGVLKSLVDFYLGHKQTDSIDISSDNEDNSSEEALSVEKMRPASLDKMIALCASLVERSRGADFRLRLSPKDFSAIAGGKGFPFLYQQIKDGINPHQTKHLIHALCRWDERLATQIIGMLFASVTKHTELCAPFFKLLTLLTETQGGPVGLPCFTQLILPRMWDAAEYCPQSVLDWLSLQATKNKIAHAWILQSAEKWLEQFLLAHDNTRVRNAAAFLLVALVPSQPFRANFRAHSQHKLLALNPHSYRDINSDAQAVLHQVITLLLRLLRPARVYADIGAHGTTKLTAYFNLLSYCMVSKTEKLMASSYMRSLWELFHPRLSEPSVPAHHNKHALLTFWHHSLVDCPENAAQVANCPEITRNIAFNYILADHDDAEIVTYNRSMLPAYYGLLRLCCEQSRALTRQLSQHQNLQWAFKNITPHPTQYAAAVDELFKLMALFATRHPDASEQEKLDVTQFRRAVIVSYTSSLDARVSWSTLISALKILVDNEEDRTMVIFNGGIEMCFEALHTLHSMHHEATACHVAGDLFDLLGEMLLLLATLRTRTDSPAQKKQQQQQQQLEQQLQLQQQQMLQKQQQQSQSQTQTPQSPQQKEKQLQQQMQQHLQLQQLQQMQFQQQHFLRQQHQHSALAKALPDAVKRLATLLNTFNSPEISRMALEVLKELVRNPSLETISILAPILINCHLSVANAPNAIGPLGPYFPRRGAKHTPWPLGAKNSPRPPRPMVQMCIALAELTPRGLDADYDVQVESFYRPYHDFIDVMMRMCVNTGTLNDTLVKLQCLVAIESTPLHFTYFPKFWVGIHNNALTHKYVELLVKNQLLVEYLHNVLRDERSMLKDACVREFLELYYHKVAAQLPVARMIYTINYGMHSKDDIDELCGDLFAIRIIAQATGVPASVRKELRGSLRALQNKSERFRKESERELSSSPYLPDPFPNKKQKRDSQKIKEKEHPQPESEKETSTENDKPSDVSMESSGNAEQATDSTKPPTPAGSDDEQMDKTSVPSSDDETELEDELQPTPKRNKKASNKKTAQDRVNEEREKRLITLITMESYIQSIFAILKRDASVPSSGATKEPSEEPCGEASTSAAAAVKLSRPKGACTPPEPITDVNDTRCNIDTETEAEADEQSPKTSQTNGSQQNESPPAATSADTAPANPSPAPAAAVASTSQAASPTQI

Target ORF information:

RefSeq Version	NM_001275993.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster puffyeye (puf), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275993.2

ORF Insert Sequence:

ATGTGCGAGG TGTGCGCCGA CTTCCAGAAT CTCCTGGAGC TCTATGAGGT GCGCGTGGCC 
AGCTCGGATC TGAAGTTCCA GCTGCTGCTG AAGAGCGAGA TCGAGACCAC CTTCAACTAC 
ATCCAGTCGT GGCCGCAGCG TCAGTGCATG TGCCTGTACC GGGACACCAA GAACTATGAC 
CGTTTCAACC TGGTAGTGCA GTCGCTCATC TGTCTGACGG TGCAGCATCT GAAGCACATC 
GACCACCTCA TCGACAACTA CAAGCGGCTA ACGGCCAGTG CTGCCCAGGT GGTGGCCCAG 
GCCCAGCAGC AGCGTGAACA GCAGCGGGAC GAAGCGTCAG CCCAGGCAGA GGCAAAGGAA 
TCATCCGCGC CAGCGGAAGA ACCTAAGAAG GAGGAGCCAT CTGGGTCCGC CGGTGAGGAG 
GCTCAGGGCT CCGGCGACGG GCCAGCCAAA AAGCCGCCAG TTGGCCCCTG CACTCCCCCT 
CCCCCGCAAA CGGCCAATCC TCAGCACAAA TCCCACCTGC AGTACACAGA GGAGCCCTGG 
ATCCTGCCCG AGGTGGAGAA ACTGTTGGTG CTCGTTTCAA AGGTGTTCCT GCTCAACTTC 
CCGCTGTACA TCGCCCACAA ACATGGCATG CACTCTCGGC TGGACGACCT GCAGGCTGAG 
GAGGCCCACC ACCTGGCCCT CATCTGCGAT CTTCACGACA ACGACCTGCC CATCTATCTG 
CTGCGGAATG TGAGCCTGTT CTGCAACTCG GGCGGCTTTG GCGCCATGTC GCTGTGCTTC 
GAACATCCCG ATCTGCCAGT GTCCACCGCT CACTCGATGA CCGCTGCCGT CTCAAATGTG 
AAACTTTGGC TGAACTATCA CTGTAATACT CAGCTCTTCG TGCCGCTGCG AAGTCGGATA 
CTGCAGTACA TGTGCAAGCT GTCGGATCAG AGCCTCCGAT CTGCCGCAAC CCGCGCGATG 
GCCGATTTTG TGTGGTCCTC TATGCGAGAC CCACTGGATG TTGCTGTCAA CTTCGACACC 
GAAGGATTGG CCCTGGCCTT TAAGTACTTC ACATCGACCA CGCTAACCAT GCGACTGGCA 
GGCATGGCCC AGATCAACGC ACACATTAAT CTATTTAACG AGATCTGCAC CACCGAAACG 
GTCAACGAAG TGGAGCTGTT CGGGCAGCGC ATCGCGAACT GGCTGACTGA AAATCATATT 
GTACAGCACC TGTTTGGCCC GAATCTGCAC GTAGAAATCG TTAAACAGGC ACACGTGCTG 
CTCAACTTTT TGGCGGTAGA GAACCAGATC TCTGAAGAGG ACATCAAGCT GATCTGGCAA 
GCCACCCAGC TGAAGCACTG CTCAAAGACG ATCTTCGACA TCCTGCCTTC GCTGGTGAAG 
AATCTGACCC CGCGCCCGGC AATGCACCTT TACTCCCTCC TTTGCCGCAT GGATCCCAAG 
GAGCACACTG AGCAGAGCAT ATACATAGCC TCAGCGCTAA CAAAACAGCT GTGGACTCGT 
GATACCTCAC GCAGTCAGAT GAACCTGATG CAAGACCACC TGCTGGGGTC CAACGTCACC 
GCTTCCAGCT CTGACAGCGG CAGCATTGAG GGCAGCAACA CAGAGGACGA CCACGTGGGC 
GCCGACGACA GTAGCATTGC CAGCGGTGGC GGCGGCGGAG GTGTTGGTAA CAAGAGTCCA 
ATCGATGGAG TTACTCCATG CAAGCAGGCC CGGCACAGGC GGCACATCTG TGACCCCACC 
ACCGAGAAGG GAAAGCAAAT AAGTCCCGAG GATATGGCCA AAGTAGACTT GGTGAACAAA 
CGGATTGTGA ACATTATCGA CAACACAAGC AGTGAAGAGG AGCTGCAATC GCGGGCCGCA 
CTGGAGCTGC AACTGCATCG CAGCCGCAAA AAGACGAGCA ACAAGCGACG GCGACAAAAA 
ACCAACAAAC AGATCATTCT GCCCCATGAG CTGGTCGAGA TTTGGGACGG AGTTGAAGAT 
GTGCCCAGCA GTGACGAGGC TGATGGCGAA GCCGATGGTG ATGGCGAGGG CGAACTTTTA 
GCGGACAGCG ATGAGTGCAG CGATGGTGCT ACGAGTCAGC TGGTACCCGA CGCCGTGCTA 
AAGCATCTGC AGGGAGAGGG ACCTTTTATC AGGGCCATCG AGACCAATAT CAATGAGCTG 
CTCAGTGGTG CCGAGAACGA TGGCAGTTAC TCATCCCCGA TGAGTAACAA ATCTGAAAAG 
AACCTGGCTG ACTTCGATGA CGAGGACGTG TCGCCTTGCG AGGAGGAGCT GGCCCAGCTG 
GTCAGCTCCC GCGCCAATTG TTCGGACGTT CCACCAGCCT TTGCAGCCGC TGCCGCCGCC 
ATGATGGTGG CCCAGTCGGC GATGGCCCTT CAGAAGTCTG GAGAGTCGAA TGTGGCTGCT 
GCTGCCGCTG CTGTGGCCGC TGCTGCAGCG GCTACCGGAA CTGCACAGCA AACGCTGGTG 
GCAATGAACC GGCAGGCCAG CGTTGCTGCA GCTGCCGCCG TGGTTGCTGC GGCCAAGGCA 
AAATCGGACT CAGATGTCGA TCTGATGGAT GTGGTTTCCG GCGGAAAGCA GCACCATTCA 
CCAAAGGCGA GCCAGGGCAG CTCTACGTCC GGGAGTACAC CGGTGCAGCC CTCGTTCAAG 
CTAAATGATG TCTGCCAGCC CGGAAACACA CTGCTTTGGG ATCTGCTTCA AGACGATAAA 
ATTGGCCAAC TAGGCGAGTC TCTGGCTCTA GAGGCGGAGA AAGCTCTGGC CACTCTCCTG 
TGCTTCAGCA TGGACCGCCA ACTGCGAACG AAATTCATCG AGGGTTGTCT GTATAACGTC 
GCCAACAACC GGAGTGTTAT TGTCAGTCTG CGACTGCTGC CCAAACTGTT CGCCTCGTTC 
CAACAGTTTC GACCCTCGGA CACGCACTCT ATGACGATGT GGGCAGAGCG AAATCACCGG 
ATGATGCAGT GCTTTTTCAA TAATATACGT CACTATGCGC GTCGCCATGC CGAGGTACTG 
ATCACCCAAA ACGGAGAGCA GCAGCAGCAG CAGCTGGGCG GACAACTCTA CTCGCACAAG 
ACACAAGTTT CTGTAAGACT GCAGTTCCTA TCATCGATAT TCTCGACGGT GGGATCCCCG 
AAAAGCTTCC GCCTAACGCT GGAGCAGCTT GACGCGCTAT GGGAGTGGCT GGCCCACGAT 
CCGGAGTGTG CCGACTGCTA CTTTTCGTGG CTGCAGGCCC AGGCCAAAGG CGGTGATCAG 
CATGCTCTGG GCATTGAAGC ACTTCAGCAT TTGTATTTGA AGAAACTCCC AGAGCTGCGA 
CCCGAGGAGT TCTCTATGGT TGCGTTGGGA CTATTTCAAC AGCTCTGTAG CTTCGCCCGC 
ATCGCCATGG CCGAGTACGA CAATCATAGC GACCAGATCT CGGCGAGCGC CAGTGCCGTT 
GGCATGTACC ATCTTTGGAA GATAGCGTTG CGTGCCCAGA GCAACGATGT CTCCTTGGCG 
GCCATACAGT ACATCAACAT GTACTACATG GGTCAACAAT TGCGCCTGGA GAAGGAGTTC 
GTTTCACAGT GTATGGAAAA CCTAGTTCAA GCGGCCACGG CTTTGGAAAG CATCGATGAT 
GAGAATGCTT TGATGCGTGT CCAGCGTGGC CTGCTCTTGC TGAACACCCA TTTGGATACG 
TTCCGGCGTC GATATGCGTT TCATCTGCGC CGCTGGGCCA TCGAAGGCAA AGGGATTGGA 
TCGCACAGTA ACCTTAAAAA TGAGGGAGCG GGTCCACCAC TACGAATAGT GCTGCAACAG 
GCAGGACTTT CGGAAAAAAG CCTTTTGCAG ATGCACGCGT GTGACTTAAT CGCCGACCTC 
AAGGCTGAGG TGTCCAAGTG GTGGGAGAGC CTTCAAACTG GACTAGCTGC TCCTGTGCTC 
GGTCTGCTGC TCAGCGATGG ACCGCTACGC ATTATCACAC AGGGACAGGA ATTGACCTCG 
GATTACGATG AACGGAGTCT CGGAGATGCT GGTTTCAAGG ATAACCAGAT CGTCTACGTA 
TCGTTAGGTG GACGTGGAGC CAGGCGAAAG GAATCCAATT TGGAGCATCC GTCAATGCTG 
CCGCCCCCAC CAAAGGAGTG TTTGCCCACG GTGCTCCTGC TGCAGCCAAA GTACTTCGAG 
AAGCTATTTT GCCTGATGCA GACCCTGGGC GATATGCAAC CGCAAGCGTC GACTGTAAAT 
CCCCAGCACC ACACAAAGGC GCAATTGCTC TCGCGACGCG TATGGGATAT CCTCGCCATG 
CTGCCAACTA ATCCGCACAT TCTAGACGCC TTTAAGAGTC TGGTTACGGA CCTAAGCGAA 
CTCGAGCAGC TTGATGCTGG CGGAGAGGAG GAGCAACTGG CCACCAAGCG CAAGCAAATC 
AAGCAGAAGT TCCGAGATCT TCTCGACCCG AACAACTTGC AAAAGTTCAT GTACTCCCTC 
CATATTGTGG AGAGTCTGGC CCTAACAAGT TCCAGACGTG GTGAATCCAA CGGCAATGTG 
GCCATGGGAA ATACACCGGA ACAGGTACGG GTGAAGAAAT CCTCGATGGG AAGACGGAGA 
AATAGCAACG AGCAGCCGCC ACCGCCGCCG CCAGAAGTCA AGATGAGCAA GGAGCAACTG 
TGCGAGCTGG AGGCCCCACT GACGCCCACA CCCTCGACTG GCTTGCAGGA TGTGGAAACG 
GAGGCCAGCA GTAGCAGCGG TGGCGACAAA GAAAACCAAC CGAAACAACA CAGCAAGCGT 
CAGAAGAAAG GCGAAACTTT TGAACAGGAA AAGGAGCGCC CAGTGGGTTG CTCCACGCCG 
CCGTCTCCAA CTCCTCCGCC ACCAGCACTT TCGGTAGTGG AAAGAGGTGA CAATAAGTGG 
AGTGAGGCGT TCGTCAAATG TGGTGGCCTT CGTCATCTGT ACGAAATCTT TAGTGAGGGC 
CAACTACAAC AAAGCGCACA TCCCAAGGAG TTGGCTTTAA ATGAATGGCG ACACGATTGC 
CTGGCCAGCC TGCTGCGCAT TCTCTGGCTT CTTGGATTCG AGGAACTGCA GTCGGCGGAT 
GCCCATGTTC TAATGTCACG TCCGCACCCG TTTATGCTAC AGTTGATGGA GGTACCACAG 
TGCCTGACCC GACTCTCATC GATCCTTAAC GACGAAGTTC ACCAGCAGCA TCAGGCTTCT 
TCGGCCAATC CACTGGTATT CCCCTATCAG TTCCAGCACT TGCGTACTGG CTTTTGGGGC 
AGGGCCCAGC TCATCCAGTT CGCTATGAAC ATTCTAGTGA GTTTTGTGCA CGCCTCAGCG 
GAAGCGAGAA GGCTTCTCTG GGCCCCAACT GGTACCGATC ACTGTCGATG GCTGCAGAAG 
TTCATCCTGG AGGATCCCGA ACCGGCGGTA CGGCGTGAGA TATGCGCTGG TCTTTATCGC 
ATTTGCTTAG GAAACGCACA TAGCTATCGC CTCTTACTAG CACCACTGCT GCACAAGCTC 
ATCGCCCTGT TGCCATTGGC CGAGCAGATG AGCTCTGGCA ACCAGCACAC GCAGTTTTTG 
CTCTCGGAAG AGGGCAAGGA TCCCTACGGA CCCGCCTGCC GTGACTACTT TTGGCTGCTG 
GCCAGATTGG TGGATACTCT CTCCCCGGAA ATGGTGGCCG AGGAGCACAT CGACATTGAA 
ATGCTGTGCG AAAGCATCTC GCAGAGCATT CTCACCCGAG AGTATTATGA ACTGCGCCAT 
GGTTACCAGG ACGATGGCTT GGTGGGTCTC CTAAATCTCA TGTCCAATTT GATCAAGTAC 
GACACCACAT TCAAGTACAC ACCCAAGGCG TTATCCTTTA TTGAGCAACT GATTGGCTTC 
CTGTTTGACA TGCCCTCGCC GGCAGATCGC CAGAAGCCCA AGTGCAAGTC GGCGTCGTCT 
CGAGCATCTG CCTACGATCT GCTGGTTGAA CTTTGCCGTG GATGCGCCAC AAACTATGCA 
TATCTGCATG GACGTCTGTT GGCACAGCAC AAATCCGGAC CCAAGCAGCC GTATCCCTGG 
GACTATTGGC CGCGCGATGA AGGCAGAGCT GAGTGCGGAT ATGTGGGGCT AACTAATTTG 
GGAGCCACTT GCTATATGGC CTCTTGCGTC CAGCACTTGT ACATGATGCC ACAGGCGCGA 
GCAGCTGTTT TGCGAGTTCC ACCCAACGCC GCCCGTAAGC ATGGGCCCAC ACTTCTGGAA 
CTACAACGGA TGTTTGCCTA CCTACTGGAG TCGGAACGGA AGTCTTACAA CCCGCGAAGC 
TTCTGCCGGG TGTACCAGAT GGATCACCAG CCGCTAAATA CGGGAGAGCA AAAGGATATG 
GCAGAGTTCT TTATCGATTT GGTGTCCAAA CTGGAAGATA TGACGCCTGA CTTGAAGCAC 
CTGGTGAAAA GGCTCTTCTG TGGATCCTTG AGCAATAATG TCGTCTCCCT GGATTGCGGT 
CACGTTTCCC GCACGGCCGA GGACTTTTAC ACGGTTCGCT GCCAGGTTGC AGATATGCGC 
AATCTGCAGG AATCCCTGGA CGAAGTCACC GTTAAGGATA CCCTCGAGGG CGACAACATG 
TACACCTGCT CGCAGTGCGG CAAAAAAGTA CGAGCCGAGA AGCGGGCTTG CTTCAAGAAG 
CTTCCCCAGA TCTTGTGCTT CAACACCATG CGCTACACCT TCAATATGGT CACCATGCTC 
AAGGAGAAGG TCAACACGCA CTTTTCCTTT CCGCTACGCC TGAACATGTG TCACTATGTG 
GAGAAGACCT TGATGCCGCA GCAGTACAAA GAGGAGCGAG AAAGGCGCCA AAAGGAGAAG 
GAAGGAGCAG ATGGTAGTGG CGATGGGAAT GACAACGAAA AGGCAGAGGC CACGCTAGAT 
GACGATATTG AAGAATGCTA CGAGTACGAA CTGGTGGGCG TCACTGTCCA CACGGGCACA 
GCGGATGGCG GCCACTACTA CAGCTTTATA AAGGAGCGGA CGAAGACTAG TTACCATACG 
CACGAGCGGT GGTTTCTTTT CAACGATGCC GAAGTGAAGC CTTTCGATCC CTCACAGATA 
GCGGCGGAGT GCTTTGGCGG CGAGATGACG AGCAAAACCT ACGACTCAGT GACGGAGAAA 
TATCTGGACT TTAGTTTCGA GAAGACCAAC TCTGCCTACA TGCTGTTCTA CGAGCGCCGA 
CTGCCAGAGC ATCTGCAGCG CCGGCATTCC GAGCTGCTGG TTACACCCAC GCCCAGTCCC 
ACAGTGGAAG AGAAGTCCGA GGCGGAGGAG CCCACTAAAA TGGAGACAAG CTCTAGCGAA 
ATCAAGGCAG ATGTGGATGT GGAAGTGGAA GTTGAGGAGA AGGACAAGGA GAAGCCTGCG 
CAGACTGACA CAGAATCTAA GGAAACGCCT GCAAAAGAGG AGATCGCGGA TGACAAATCG 
AAGCAGGATG AGCCTGAGGA AAAGAAAATC GAAAAGCAAT CCAGAGAGGG CGAAGAGAAA 
TCGGAAACGG ACGAAAAGCC CACGGAGATG ACGACCGTGA GCACTGAGGA GGAAAAGCAG 
CCAACAGCCA ATTGTGATAA TCACCAGCAA AACAACAATA GCAACAGCAA AGCCAGCAAC 
GATCAGCAAC CATCCACGTC GAAGGCTGCC CAGAAGCTAC AACTATTTCG TCCATTGTTG 
AACAAGGAGC TCGAGGATTG GATTTGGCAG GACAATCGAC AGTTCCTGCA GGATCGCAAC 
ATTTTCGAGC ACACATATTT CAACTTTATG TGGCAGATAT GTGGGCACAT ACCCCAATCA 
TTAATATCAG AGACGGACGT CACCTGCATG GCAGCCAAGT TGTCTGTGTC CTTTTTCATT 
GAGACTTTTA TTCATGCTAA GGAAAAGCCC ACTATGGTGC CCTGGGTGGA GCTGCTGACC 
AAGCAGTTCA ATGCCAGCCA AGAGGCCTGC GAGTGGTTTC TGTCGCACAT GTCGCAGGAG 
CCCTACTGGC CGGTCCAGGT GCTAATCCAG TGTCCCAACC AAATGGTGCG TCAAATGTTT 
CAGCGTTTGG TAATCCATGT CATCCAGCAA CTACGTGCCT CACATGCTCA TCTGTATCTG 
GAAGTGGAGA CAGACGAGGA TGACAAGGAG TTAATAGGCC AAGCCTCTTG CGTCACCCGT 
TTCATTGGTT CGCTCATCTC GCTCCTGGAG CACGGTGCCC GAGCGAACCT GCGGCACTTG 
TCCGAGTACT TTGGACTGTT GTGCGAGTTC TCACGGATGG GAGATGAGGA AGCCATGTAC 
TTGCTGCGCA TCGGTGTACT CAAGAGCCTG GTCGACTTCT ATCTGGGTCA CAAGCAAACA 
GACAGCATAG ACATAAGCTC TGACAACGAA GACAACTCCT CTGAAGAGGC GCTGTCCGTG 
GAGAAGATGC GTCCGGCCTC GCTGGACAAG ATGATTGCTC TGTGCGCCAG CCTAGTGGAA 
AGATCAAGAG GAGCCGACTT CCGGCTGCGA CTCTCGCCCA AAGACTTCTC TGCAATCGCC 
GGCGGCAAGG GTTTTCCATT TTTGTACCAA CAAATAAAGG ACGGCATTAA TCCGCACCAG 
ACAAAGCATT TAATACACGC GCTTTGTCGC TGGGATGAGC GCCTGGCCAC GCAGATCATT 
GGCATGCTCT TCGCGTCGGT AACCAAGCAC ACGGAGCTGT GCGCGCCATT CTTCAAGCTG 
CTAACTCTGC TCACCGAGAC GCAGGGCGGT CCAGTGGGCC TGCCTTGTTT CACCCAGTTG 
ATCCTGCCAC GAATGTGGGA CGCCGCCGAG TACTGTCCAC AGTCGGTGCT CGACTGGCTC 
TCGCTGCAGG CCACCAAGAA CAAGATCGCC CACGCCTGGA TACTTCAATC GGCCGAGAAG 
TGGCTCGAGC AATTCCTGCT GGCCCACGAT AACACTCGTG TTCGGAACGC TGCCGCCTTT 
CTGCTGGTGG CACTGGTACC CTCCCAGCCA TTCCGGGCCA ACTTCCGCGC CCACTCGCAA 
CACAAGCTGC TGGCCCTCAA TCCGCACAGT TATCGCGACA TAAATAGCGA TGCCCAGGCG 
GTGCTGCACC AGGTGATCAC GCTGCTCCTG CGATTGCTGC GTCCAGCGCG CGTCTATGCG 
GATATCGGAG CCCATGGCAC GACCAAGCTG ACCGCGTACT TTAATCTGCT CAGCTATTGT 
ATGGTTTCAA AGACTGAAAA ACTAATGGCA AGTTCGTACA TGCGCTCCCT GTGGGAGCTG 
TTCCATCCAC GCCTCTCGGA GCCCAGTGTT CCGGCCCATC ACAATAAGCA CGCACTATTA 
ACCTTCTGGC ACCACTCACT GGTGGACTGT CCGGAGAATG CTGCCCAGGT GGCCAACTGC 
CCGGAGATTA CGCGCAACAT TGCATTTAAC TATATACTGG CCGATCATGA TGACGCGGAG 
ATAGTCACCT ACAACCGTTC CATGCTGCCC GCCTATTATG GACTACTTCG TCTGTGCTGC 
GAGCAGAGCC GTGCACTCAC ACGCCAATTG TCCCAGCATC AGAATCTTCA GTGGGCATTT 
AAAAACATCA CACCGCATCC CACGCAGTAC GCGGCCGCCG TGGACGAGCT TTTCAAGTTG 
ATGGCTCTGT TCGCCACCCG TCATCCGGAT GCTAGTGAAC AGGAAAAGCT TGATGTGACC 
CAGTTTAGGA GGGCTGTGAT TGTGTCCTAC ACCAGTAGTC TGGATGCACG TGTCTCTTGG 
TCCACGCTGA TCAGTGCTCT TAAGATACTC GTGGACAATG AGGAGGACCG CACGATGGTG 
ATCTTCAACG GCGGCATTGA GATGTGTTTC GAGGCGCTGC ACACGCTACA TTCCATGCAC 
CACGAGGCCA CCGCCTGCCA CGTGGCCGGA GACCTATTCG ATCTCTTGGG CGAGATGCTA 
CTCCTCCTAG CCACTCTTCG AACCCGCACA GACAGTCCTG CTCAAAAGAA GCAGCAGCAA 
CAACAGCAGC AGCTGGAGCA ACAGTTGCAG TTGCAGCAGC AACAGATGCT GCAGAAACAA 
CAGCAGCAGT CGCAAAGCCA AACGCAAACA CCGCAGTCGC CGCAGCAAAA GGAAAAGCAG 
CTGCAGCAAC AAATGCAGCA GCATCTGCAG CTGCAACAGT TGCAGCAAAT GCAGTTCCAG 
CAGCAACACT TCCTGCGCCA GCAACATCAA CACTCGGCGC TGGCAAAGGC ACTGCCGGAT 
GCAGTGAAGC GACTGGCTAC GCTACTCAAC ACCTTCAATT CGCCAGAGAT CAGTCGCATG 
GCCTTGGAGG TGCTCAAGGA GCTGGTGCGC AATCCCAGCC TGGAAACCAT TAGTATTCTG 
GCGCCCATTC TTATCAACTG TCATCTGTCC GTGGCGAATG CTCCCAATGC GATCGGTCCT 
CTGGGACCGT ACTTTCCGCG TCGCGGTGCG AAGCATACGC CTTGGCCATT GGGAGCCAAG 
AACTCACCCC GTCCACCGCG GCCCATGGTA CAGATGTGCA TCGCTCTGGC TGAGCTGACA 
CCACGCGGTC TCGATGCCGA TTACGACGTT CAGGTGGAGT CCTTTTACCG ACCCTACCAT 
GACTTTATCG ATGTCATGAT GCGCATGTGC GTCAACACGG GCACTCTGAA CGACACGCTA 
GTCAAGCTGC AATGCTTGGT GGCCATCGAG TCCACGCCGC TGCACTTCAC CTATTTCCCC 
AAATTCTGGG TGGGCATACA CAACAATGCT CTGACGCACA AATACGTTGA GCTGCTGGTG 
AAAAATCAAC TGTTGGTAGA GTACCTTCAC AATGTCCTTA GGGACGAGCG AAGCATGTTG 
AAGGATGCCT GCGTTCGTGA ATTTCTGGAG CTCTATTATC ACAAGGTTGC CGCCCAGCTG 
CCAGTGGCCC GTATGATCTA CACAATCAAC TATGGTATGC ACTCCAAGGA CGACATTGAT 
GAGCTGTGTG GCGATCTGTT CGCCATCCGA ATCATTGCCC AGGCCACTGG AGTGCCGGCC 
TCAGTGCGCA AGGAGTTGCG AGGATCGCTG AGGGCGCTTC AGAACAAGAG CGAGCGGTTT 
AGGAAGGAAT CGGAGAGGGA GCTATCATCA TCTCCCTATT TGCCAGATCC ATTTCCGAAC 
AAGAAGCAGA AACGTGACTC GCAAAAGATC AAGGAAAAGG AGCACCCTCA GCCGGAGAGC 
GAGAAAGAGA CCTCAACGGA GAACGACAAA CCATCAGATG TTTCCATGGA ATCCAGCGGT 
AACGCGGAGC AAGCAACGGA CAGCACAAAA CCACCAACTC CTGCAGGCAG CGACGATGAG 
CAGATGGACA AAACTTCAGT TCCCTCGTCC GATGACGAGA CAGAGTTGGA GGACGAGCTG 
CAGCCGACGC CCAAGCGCAA CAAGAAGGCT TCGAATAAGA AGACCGCCCA GGATCGTGTC 
AACGAGGAGC GCGAGAAGCG ACTTATCACG CTGATCACCA TGGAATCATA TATCCAGAGC 
ATTTTTGCCA TCCTTAAGAG AGATGCCAGT GTCCCCAGTT CGGGAGCGAC TAAGGAGCCC 
AGTGAGGAAC CGTGTGGCGA GGCATCCACT TCGGCGGCTG CCGCTGTAAA GTTATCTCGT 
CCCAAAGGAG CCTGTACTCC GCCGGAGCCA ATTACCGACG TAAACGATAC GCGTTGCAAC 
ATAGATACTG AGACAGAGGC GGAGGCGGAT GAGCAATCTC CAAAGACGAG CCAGACAAAT 
GGTAGCCAAC AGAACGAATC CCCGCCAGCG GCGACCAGTG CAGACACTGC ACCCGCGAAT 
CCTTCACCAG CTCCCGCGGC CGCCGTTGCG TCCACATCCC AGGCGGCAAG TCCAACACAG 
ATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

puf ( NM_001300579.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287508.1 Drosophila melanogaster puffyeye (puf), transcript variant I, mRNA.

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CloneID	OFa22151
Clone ID Related Accession (Same CDS sequence)	NM_001300579.1
Accession Version	NM_001300579.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 11766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	puffyeye, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCGAGG TGTGCGCCGA CTTCCAGAAT CTCCTGGAGC TCTATGAGGT GCGCGTGGCC 
AGCTCGGATC TGAAGTTCCA GCTGCTGCTG AAGAGCGAGA TCGAGACCAC CTTCAACTAC 
ATCCAGTCGT GGCCGCAGCG TCAGTGCATG TGCCTGTACC GGGACACCAA GAACTATGAC 
CGTTTCAACC TGGTAGTGCA GTCGCTCATC TGTCTGACGG TGCAGCATCT GAAGCACATC 
GACCACCTCA TCGACAACTA CAAGCGGCTA ACGGCCAGTG CTGCCCAGGT GGTGGCCCAG 
GCCCAGCAGC AGCGTGAACA GCAGCGGGAC GAAGCGTCAG CCCAGGCAGA GGCAAAGGAA 
TCATCCGCGC CAGCGGAAGA ACCTAAGAAG GAGGAGCCAT CTGGGTCCGC CGGTGAGGAG 
GCTCAGGGCT CCGGCGACGG GCCAGCCAAA AAGCCGCCAG TTGGCCCCTG CACTCCCCCT 
CCCCCGCAAA CGGCCAATCC TCAGCACAAA TCCCACCTGC AGTACACAGA GGAGCCCTGG 
ATCCTGCCCG AGGTGGAGAA ACTGTTGGTG CTCGTTTCAA AGGTGTTCCT GCTCAACTTC 
CCGCTGTACA TCGCCCACAA ACATGGCATG CACTCTCGGC TGGACGACCT GCAGGCTGAG 
GAGGCCCACC ACCTGGCCCT CATCTGCGAT CTTCACGACA ACGACCTGCC CATCTATCTG 
CTGCGGAATG TGAGCCTGTT CTGCAACTCG GGCGGCTTTG GCGCCATGTC GCTGTGCTTC 
GAACATCCCG ATCTGCCAGT GTCCACCGCT CACTCGATGA CCGCTGCCGT CTCAAATGTG 
AAACTTTGGC TGAACTATCA CTGTAATACT CAGCTCTTCG TGCCGCTGCG AAGTCGGATA 
CTGCAGTACA TGTGCAAGCT GTCGGATCAG AGCCTCCGAT CTGCCGCAAC CCGCGCGATG 
GCCGATTTTG TGTGGTCCTC TATGCGAGAC CCACTGGATG TTGCTGTCAA CTTCGACACC 
GAAGGATTGG CCCTGGCCTT TAAGTACTTC ACATCGACCA CGCTAACCAT GCGACTGGCA 
GGCATGGCCC AGATCAACGC ACACATTAAT CTATTTAACG AGATCTGCAC CACCGAAACG 
GTCAACGAAG TGGAGCTGTT CGGGCAGCGC ATCGCGAACT GGCTGACTGA AAATCATATT 
GTACAGCACC TGTTTGGCCC GAATCTGCAC GTAGAAATCG TTAAACAGGC ACACGTGCTG 
CTCAACTTTT TGGCGGTAGA GAACCAGATC TCTGAAGAGG ACATCAAGCT GATCTGGCAA 
GCCACCCAGC TGAAGCACTG CTCAAAGACG ATCTTCGACA TCCTGCCTTC GCTGGTGAAG 
AATCTGACCC CGCGCCCGGC AATGCACCTT TACTCCCTCC TTTGCCGCAT GGATCCCAAG 
GAGCACACTG AGCAGAGCAT ATACATAGCC TCAGCGCTAA CAAAACAGCT GTGGACTCGT 
GATACCTCAC GCAGTCAGAT GAACCTGATG CAAGACCACC TGCTGGGGTC CAACGTCACC 
GCTTCCAGCT CTGACAGCGG CAGCATTGAG GGCAGCAACA CAGAGGACGA CCACGTGGGC 
GCCGACGACA GTAGCATTGC CAGCGGTGGC GGCGGCGGAG GTGTTGGTAA CAAGAGTCCA 
ATCGATGGAG TTACTCCATG CAAGCAGGCC CGGCACAGGC GGCACATCTG TGACCCCACC 
ACCGAGAAGG GAAAGCAAAT AAGTCCCGAG GATATGGCCA AAGTAGACTT GGTGAACAAA 
CGGATTGTGA ACATTATCGA CAACACAAGC AGTGAAGAGG AGCTGCAATC GCGGGCCGCA 
CTGGAGCTGC AACTGCATCG CAGCCGCAAA AAGACGAGCA ACAAGCGACG GCGACAAAAA 
ACCAACAAAC AGATCATTCT GCCCCATGAG CTGGTCGAGA TTTGGGACGG AGTTGAAGAT 
GTGCCCAGCA GTGACGAGGC TGATGGCGAA GCCGATGGTG ATGGCGAGGG CGAACTTTTA 
GCGGACAGCG ATGAGTGCAG CGATGGTGCT ACGAGTCAGC TGGTACCCGA CGCCGTGCTA 
AAGCATCTGC AGGGAGAGGG ACCTTTTATC AGGGCCATCG AGACCAATAT CAATGAGCTG 
CTCAGTGGTG CCGAGAACGA TGGCAGTTAC TCATCCCCGA TGAGTAACAA ATCTGAAAAG 
AACCTGGCTG ACTTCGATGA CGAGGACGTG TCGCCTTGCG AGGAGGAGCT GGCCCAGCTG 
GTCAGCTCCC GCGCCAATTG TTCGGACGTT CCACCAGCCT TTGCAGCCGC TGCCGCCGCC 
ATGATGGTGG CCCAGTCGGC GATGGCCCTT CAGAAGTCTG GAGAGTCGAA TGTGGCTGCT 
GCTGCCGCTG CTGTGGCCGC TGCTGCAGCG GCTACCGGAA CTGCACAGCA AACGCTGGTG 
GCAATGAACC GGCAGGCCAG CGTTGCTGCA GCTGCCGCCG TGGTTGCTGC GGCCAAGGCA 
AAATCGGACT CAGATGTCGA TCTGATGGAT GTGGTTTCCG GCGGAAAGCA GCACCATTCA 
CCAAAGGCGA GCCAGGGCAG CTCTACGTCC GGGAGTACAC CGGTGCAGCC CTCGTTCAAG 
CTAAATGATG TCTGCCAGCC CGGAAACACA CTGCTTTGGG ATCTGCTTCA AGACGATAAA 
ATTGGCCAAC TAGGCGAGTC TCTGGCTCTA GAGGCGGAGA AAGCTCTGGC CACTCTCCTG 
TGCTTCAGCA TGGACCGCCA ACTGCGAACG AAATTCATCG AGGGTTGTCT GTATAACGTC 
GCCAACAACC GGAGTGTTAT TGTCAGTCTG CGACTGCTGC CCAAACTGTT CGCCTCGTTC 
CAACAGTTTC GACCCTCGGA CACGCACTCT ATGACGATGT GGGCAGAGCG AAATCACCGG 
ATGATGCAGT GCTTTTTCAA TAATATACGT CACTATGCGC GTCGCCATGC CGAGGTACTG 
ATCACCCAAA ACGGAGAGCA GCAGCAGCAG CAGCTGGGCG GACAACTCTA CTCGCACAAG 
ACACAAGTTT CTGTAAGACT GCAGTTCCTA TCATCGATAT TCTCGACGGT GGGATCCCCG 
AAAAGCTTCC GCCTAACGCT GGAGCAGCTT GACGCGCTAT GGGAGTGGCT GGCCCACGAT 
CCGGAGTGTG CCGACTGCTA CTTTTCGTGG CTGCAGGCCC AGGCCAAAGG CGGTGATCAG 
CATGCTCTGG GCATTGAAGC ACTTCAGCAT TTGTATTTGA AGAAACTCCC AGAGCTGCGA 
CCCGAGGAGT TCTCTATGGT TGCGTTGGGA CTATTTCAAC AGCTCTGTAG CTTCGCCCGC 
ATCGCCATGG CCGAGTACGA CAATCATAGC GACCAGATCT CGGCGAGCGC CAGTGCCGTT 
GGCATGTACC ATCTTTGGAA GATAGCGTTG CGTGCCCAGA GCAACGATGT CTCCTTGGCG 
GCCATACAGT ACATCAACAT GTACTACATG GGTCAACAAT TGCGCCTGGA GAAGGAGTTC 
GTTTCACAGT GTATGGAAAA CCTAGTTCAA GCGGCCACGG CTTTGGAAAG CATCGATGAT 
GAGAATGCTT TGATGCGTGT CCAGCGTGGC CTGCTCTTGC TGAACACCCA TTTGGATACG 
TTCCGGCGTC GATATGCGTT TCATCTGCGC CGCTGGGCCA TCGAAGGCAA AGGGATTGGA 
TCGCACAGTA ACCTTAAAAA TGAGGGAGCG GGTCCACCAC TACGAATAGT GCTGCAACAG 
GCAGGACTTT CGGAAAAAAG CCTTTTGCAG ATGCACGCGT GTGACTTAAT CGCCGACCTC 
AAGGCTGAGG TGTCCAAGTG GTGGGAGAGC CTTCAAACTG GACTAGCTGC TCCTGTGCTC 
GGTCTGCTGC TCAGCGATGG ACCGCTACGC ATTATCACAC AGGGACAGGA ATTGACCTCG 
GATTACGATG AACGGAGTCT CGGAGATGCT GGTTTCAAGG ATAACCAGAT CGTCTACGTA 
TCGTTAGGTG GACGTGGAGC CAGGCGAAAG GAATCCAATT TGGAGCATCC GTCAATGCTG 
CCGCCCCCAC CAAAGGAGTG TTTGCCCACG GTGCTCCTGC TGCAGCCAAA GTACTTCGAG 
AAGCTATTTT GCCTGATGCA GACCCTGGGC GATATGCAAC CGCAAGCGTC GACTGTAAAT 
CCCCAGCACC ACACAAAGGC GCAATTGCTC TCGCGACGCG TATGGGATAT CCTCGCCATG 
CTGCCAACTA ATCCGCACAT TCTAGACGCC TTTAAGAGTC TGGTTACGGA CCTAAGCGAA 
CTCGAGCAGC TTGATGCTGG CGGAGAGGAG GAGCAACTGG CCACCAAGCG CAAGCAAATC 
AAGCAGAAGT TCCGAGATCT TCTCGACCCG AACAACTTGC AAAAGTTCAT GTACTCCCTC 
CATATTGTGG AGAGTCTGGC CCTAACAAGT TCCAGACGTG GTGAATCCAA CGGCAATGTG 
GCCATGGGAA ATACACCGGA ACAGGTACGG GTGAAGAAAT CCTCGATGGG AAGACGGAGA 
AATAGCAACG AGCAGCCGCC ACCGCCGCCG CCAGAAGTCA AGATGAGCAA GGAGCAACTG 
TGCGAGCTGG AGGCCCCACT GACGCCCACA CCCTCGACTG GCTTGCAGGA TGTGGAAACG 
GAGGCCAGCA GTAGCAGCGG TGGCGACAAA GAAAACCAAC CGAAACAACA CAGCAAGCGT 
CAGAAGAAAG GCGAAACTTT TGAACAGGAA AAGGAGCGCC CAGTGGGTTG CTCCACGCCG 
CCGTCTCCAA CTCCTCCGCC ACCAGCACTT TCGGTAGTGG AAAGAGGTGA CAATAAGTGG 
AGTGAGGCGT TCGTCAAATG TGGTGGCCTT CGTCATCTGT ACGAAATCTT TAGTGAGGGC 
CAACTACAAC AAAGCGCACA TCCCAAGGAG TTGGCTTTAA ATGAATGGCG ACACGATTGC 
CTGGCCAGCC TGCTGCGCAT TCTCTGGCTT CTTGGATTCG AGGAACTGCA GTCGGCGGAT 
GCCCATGTTC TAATGTCACG TCCGCACCCG TTTATGCTAC AGTTGATGGA GGTACCACAG 
TGCCTGACCC GACTCTCATC GATCCTTAAC GACGAAGTTC ACCAGCAGCA TCAGGCTTCT 
TCGGCCAATC CACTGGTATT CCCCTATCAG TTCCAGCACT TGCGTACTGG CTTTTGGGGC 
AGGGCCCAGC TCATCCAGTT CGCTATGAAC ATTCTAGTGA GTTTTGTGCA CGCCTCAGCG 
GAAGCGAGAA GGCTTCTCTG GGCCCCAACT GGTACCGATC ACTGTCGATG GCTGCAGAAG 
TTCATCCTGG AGGATCCCGA ACCGGCGGTA CGGCGTGAGA TATGCGCTGG TCTTTATCGC 
ATTTGCTTAG GAAACGCACA TAGCTATCGC CTCTTACTAG CACCACTGCT GCACAAGCTC 
ATCGCCCTGT TGCCATTGGC CGAGCAGATG AGCTCTGGCA ACCAGCACAC GCAGTTTTTG 
CTCTCGGAAG AGGGCAAGGA TCCCTACGGA CCCGCCTGCC GTGACTACTT TTGGCTGCTG 
GCCAGATTGG TGGATACTCT CTCCCCGGAA ATGGTGGCCG AGGAGCACAT CGACATTGAA 
ATGCTGTGCG AAAGCATCTC GCAGAGCATT CTCACCCGAG AGTATTATGA ACTGCGCCAT 
GGTTACCAGG ACGATGGCTT GGTGGGTCTC CTAAATCTCA TGTCCAATTT GATCAAGTAC 
GACACCACAT TCAAGTACAC ACCCAAGGCG TTATCCTTTA TTGAGCAACT GATTGGCTTC 
CTGTTTGACA TGCCCTCGCC GGCAGATCGC CAGAAGCCCA AGTGCAAGTC GGCGTCGTCT 
CGAGCATCTG CCTACGATCT GCTGGTTGAA CTTTGCCGTG GATGCGCCAC AAACTATGCA 
TATCTGCATG GACGTCTGTT GGCACAGCAC AAATCCGGAC CCAAGCAGCC GTATCCCTGG 
GACTATTGGC CGCGCGATGA AGGCAGAGCT GAGTGCGGAT ATGTGGGGCT AACTAATTTG 
GGAGCCACTT GCTATATGGC CTCTTGCGTC CAGCACTTGT ACATGATGCC ACAGGCGCGA 
GCAGCTGTTT TGCGAGTTCC ACCCAACGCC GCCCGTAAGC ATGGGCCCAC ACTTCTGGAA 
CTACAACGGA TGTTTGCCTA CCTACTGGAG TCGGAACGGA AGTCTTACAA CCCGCGAAGC 
TTCTGCCGGG TGTACCAGAT GGATCACCAG CCGCTAAATA CGGGAGAGCA AAAGGATATG 
GCAGAGTTCT TTATCGATTT GGTGTCCAAA CTGGAAGATA TGACGCCTGA CTTGAAGCAC 
CTGGTGAAAA GGCTCTTCTG TGGATCCTTG AGCAATAATG TCGTCTCCCT GGATTGCGGT 
CACGTTTCCC GCACGGCCGA GGACTTTTAC ACGGTTCGCT GCCAGGTTGC AGATATGCGC 
AATCTGCAGG AATCCCTGGA CGAAGTCACC GTTAAGGATA CCCTCGAGGG CGACAACATG 
TACACCTGCT CGCAGTGCGG CAAAAAAGTA CGAGCCGAGA AGCGGGCTTG CTTCAAGAAG 
CTTCCCCAGA TCTTGTGCTT CAACACCATG CGCTACACCT TCAATATGGT CACCATGCTC 
AAGGAGAAGG TCAACACGCA CTTTTCCTTT CCGCTACGCC TGAACATGTG TCACTATGTG 
GAGAAGACCT TGATGCCGCA GCAGTACAAA GAGGAGCGAG AAAGGCGCCA AAAGGAGAAG 
GAAGGAGCAG ATGGTAGTGG CGATGGGAAT GACAACGAAA AGGCAGAGGC CACGCTAGAT 
GACGATATTG AAGAATGCTA CGAGTACGAA CTGGTGGGCG TCACTGTCCA CACGGGCACA 
GCGGATGGCG GCCACTACTA CAGCTTTATA AAGGAGCGGA CGAAGACTAG TTACCATACG 
CACGAGCGGT GGTTTCTTTT CAACGATGCC GAAGTGAAGC CTTTCGATCC CTCACAGATA 
GCGGCGGAGT GCTTTGGCGG CGAGATGACG AGCAAAACCT ACGACTCAGT GACGGAGAAA 
TATCTGGACT TTAGTTTCGA GAAGACCAAC TCTGCCTACA TGCTGTTCTA CGAGCGCCGA 
CTGCCAGAGC ATCTGCAGCG CCGGCATTCC GAGCTGCTGG TTACACCCAC GCCCAGTCCC 
ACAGTGGAAG AGAAGTCCGA GGCGGAGGAG CCCACTAAAA TGGAGACAAG CTCTAGCGAA 
ATCAAGGCAG ATGTGGATGT GGAAGTGGAA GTTGAGGAGA AGGACAAGGA GAAGCCTGCG 
CAGACTGACA CAGAATCTAA GGAAACGCCT GCAAAAGAGG AGATCGCGGA TGACAAATCG 
AAGCAGGATG AGCCTGAGGA AAAGAAAATC GAAAAGCAAT CCAGAGAGGG CGAAGAGAAA 
TCGGAAACGG ACGAAAAGCC CACGGAGATG ACGACCGTGA GCACTGAGGA GGAAAAGCAG 
CCAACAGCCA ATTGTGATAA TCACCAGCAA AACAACAATA GCAACAGCAA AGCCAGCAAC 
GATCAGCAAC CATCCACGTC GAAGGCTGCC CAGAAGCTAC AACTATTTCG TCCATTGTTG 
AACAAGGAGC TCGAGGATTG GATTTGGCAG GACAATCGAC AGTTCCTGCA GGATCGCAAC 
ATTTTCGAGC ACACATATTT CAACTTTATG TGGCAGATAT GTGGGCACAT ACCCCAATCA 
TTAATATCAG AGACGGACGT CACCTGCATG GCAGCCAAGT TGTCTGTGTC CTTTTTCATT 
GAGACTTTTA TTCATGCTAA GGAAAAGCCC ACTATGGTGC CCTGGGTGGA GCTGCTGACC 
AAGCAGTTCA ATGCCAGCCA AGAGGCCTGC GAGTGGTTTC TGTCGCACAT GTCGCAGGAG 
CCCTACTGGC CGGTCCAGGT GCTAATCCAG TGTCCCAACC AAATGGTGCG TCAAATGTTT 
CAGCGTTTGG TAATCCATGT CATCCAGCAA CTACGTGCCT CACATGCTCA TCTGTATCTG 
GAAGTGGAGA CAGACGAGGA TGACAAGGAG TTAATAGGCC AAGCCTCTTG CGTCACCCGT 
TTCATTGGTT CGCTCATCTC GCTCCTGGAG CACGGTGCCC GAGCGAACCT GCGGCACTTG 
TCCGAGTACT TTGGACTGTT GTGCGAGTTC TCACGGATGG GAGATGAGGA AGCCATGTAC 
TTGCTGCGCA TCGGTGTACT CAAGAGCCTG GTCGACTTCT ATCTGGGTCA CAAGCAAACA 
GACAGCATAG ACATAAGCTC TGACAACGAA GACAACTCCT CTGAAGAGGC GCTGTCCGTG 
GAGAAGATGC GTCCGGCCTC GCTGGACAAG ATGATTGCTC TGTGCGCCAG CCTAGTGGAA 
AGATCAAGAG GAGCCGACTT CCGGCTGCGA CTCTCGCCCA AAGACTTCTC TGCAATCGCC 
GGCGGCAAGG GTTTTCCATT TTTGTACCAA CAAATAAAGG ACGGCATTAA TCCGCACCAG 
ACAAAGCATT TAATACACGC GCTTTGTCGC TGGGATGAGC GCCTGGCCAC GCAGATCATT 
GGCATGCTCT TCGCGTCGGT AACCAAGCAC ACGGAGCTGT GCGCGCCATT CTTCAAGCTG 
CTAACTCTGC TCACCGAGAC GCAGGGCGGT CCAGTGGGCC TGCCTTGTTT CACCCAGTTG 
ATCCTGCCAC GAATGTGGGA CGCCGCCGAG TACTGTCCAC AGTCGGTGCT CGACTGGCTC 
TCGCTGCAGG CCACCAAGAA CAAGATCGCC CACGCCTGGA TACTTCAATC GGCCGAGAAG 
TGGCTCGAGC AATTCCTGCT GGCCCACGAT AACACTCGTG TTCGGAACGC TGCCGCCTTT 
CTGCTGGTGG CACTGGTACC CTCCCAGCCA TTCCGGGCCA ACTTCCGCGC CCACTCGCAA 
CACAAGCTGC TGGCCCTCAA TCCGCACAGT TATCGCGACA TAAATAGCGA TGCCCAGGCG 
GTGCTGCACC AGGTGATCAC GCTGCTCCTG CGATTGCTGC GTCCAGCGCG CGTCTATGCG 
GATATCGGAG CCCATGGCAC GACCAAGCTG ACCGCGTACT TTAATCTGCT CAGCTATTGT 
ATGGTTTCAA AGACTGAAAA ACTAATGATA GGTTCGTACA TGCGCTCCCT GTGGGAGCTG 
TTCCATCCAC GCCTCTCGGA GCCCAGTGTT CCGGCCCATC ACAATAAGCA CGCACTATTA 
ACCTTCTGGC ACCACTCACT GGTGGACTGT CCGGAGAATG CTGCCCAGGT GGCCAACTGC 
CCGGAGATTA CGCGCAACAT TGCATTTAAC TATATACTGG CCGATCATGA TGACGCGGAG 
ATAGTCACCT ACAACCGTTC CATGCTGCCC GCCTATTATG GACTACTTCG TCTGTGCTGC 
GAGCAGAGCC GTGCACTCAC ACGCCAATTG TCCCAGCATC AGAATCTTCA GTGGGCATTT 
AAAAACATCA CACCGCATCC CACGCAGTAC GCGGCCGCCG TGGACGAGCT TTTCAAGTTG 
ATGGCTCTGT TCGCCACCCG TCATCCGGAT GCTAGTGAAC AGGAAAAGCT TGATGTGACC 
CAGTTTAGGA GGGCTGTGAT TGTGTCCTAC ACCAGTAGTC TGGATGCACG TGTCTCTTGG 
TCCACGCTGA TCAGTGCTCT TAAGATACTC GTGGACAATG AGGAGGACCG CACGATGGTG 
ATCTTCAACG GCGGCATTGA GATGTGTTTC GAGGCGCTGC ACACGCTACA TTCCATGCAC 
CACGAGGCCA CCGCCTGCCA CGTGGCCGGA GACCTATTCG ATCTCTTGGG CGAGATGCTA 
CTCCTCCTAG CCACTCTTCG AACCCGCACA GACAGTCCTG CTCAAAAGAA GCAGCAGCAA 
CAACAGCAGC AGCTGGAGCA ACAGTTGCAG TTGCAGCAGC AACAGATGCT GCAGAAACAA 
CAGCAGCAGT CGCAAAGCCA AACGCAAACA CCGCAGTCGC CGCAGCAAAA GGAAAAGCAG 
CTGCAGCAAC AAATGCAGCA GCATCTGCAG CTGCAACAGT TGCAGCAAAT GCAGTTCCAG 
CAGCAACACT TCCTGCGCCA GCAACATCAA CACTCGGCGC TGGCAAAGGC ACTGCCGGAT 
GCAGTGAAGC GACTGGCTAC GCTACTCAAC ACCTTCAATT CGCCAGAGAT CAGTCGCATG 
GCCTTGGAGG TGCTCAAGGA GCTGGTGCGC AATCCCAGCC TGGAAACCAT TAGTATTCTG 
GCGCCCATTC TTATCAACTG TCATCTGTCC GTGGCGAATG CTCCCAATGC GATCGGTCCT 
CTGGGACCGT ACTTTCCGCG TCGCGGTGCG AAGCATACGC CTTGGCCATT GGGAGCCAAG 
AACTCACCCC GTCCACCGCG GCCCATGGTA CAGATGTGCA TCGCTCTGGC TGAGCTGACA 
CCACGCGGTC TCGATGCCGA TTACGACGTT CAGGTGGAGT CCTTTTACCG ACCCTACCAT 
GACTTTATCG ATGTCATGAT GCGCATGTGC GTCAACACGG GCACTCTGAA CGACACGCTA 
GTCAAGCTGC AATGCTTGGT GGCCATCGAG TCCACGCCGC TGCACTTCAC CTATTTCCCC 
AAATTCTGGG TGGGCATACA CAACAATGCT CTGACGCACA AATACGTTGA GCTGCTGGTG 
AAAAATCAAC TGTTGGTAGA GTACCTTCAC AATGTCCTTA GGGACGAGCG AAGCATGTTG 
AAGGATGCCT GCGTTCGTGA ATTTCTGGAG CTCTATTATC ACAAGGTTGC CGCCCAGCTG 
CCAGTGGCCC GTATGATCTA CACAATCAAC TATGGTATGC ACTCCAAGGA CGACATTGAT 
GAGCTGTGTG GCGATCTGTT CGCCATCCGA ATCATTGCCC AGGCCACTGG AGTGCCGGCC 
TCAGTGCGCA AGGAGTTGCG AGGATCGCTG AGGGCGCTTC AGAACAAGAG CGAGCGGTTT 
AGGAAGGAAT CGGAGAGGGA GCTATCATCA TCTCCCTATT TGCCAGATCC ATTTCCGAAC 
AAGAAGCAGA AACGTGACTC GCAAAAGATC AAGGAAAAGG AGCACCCTCA GCCGGAGAGC 
GAGAAAGAGA CCTCAACGGA GAACGACAAA CCATCAGATG TTTCCATGGA ATCCAGCGGT 
AACGCGGAGC AAGCAACGGA CAGCACAAAA CCACCAACTC CTGCAGGCAG CGACGATGAG 
CAGATGGACA AAACTTCAGT TCCCTCGTCC GATGACGAGA CAGAGTTGGA GGACGAGCTG 
CAGCCGACGC CCAAGCGCAA CAAGAAGGCT TCGAATAAGA AGACCGCCCA GGATCGTGTC 
AACGAGGAGC GCGAGAAGCG ACTTATCACG CTGATCACCA TGGAATCATA TATCCAGAGC 
ATTTTTGCCA TCCTTAAGAG AGATGCCAGT GTCCCCAGTT CGGGAGCGAC TAAGGAGCCC 
AGTGAGGAAC CGTGTGGCGA GGCATCCACT TCGGCGGCTG CCGCTGTAAA GTTATCTCGT 
CCCAAAGGAG CCTGTACTCC GCCGGAGCCA ATTACCGACG TAAACGATAC GCGTTGCAAC 
ATAGATACTG AGACAGAGGC GGAGGCGGAT GAGCAATCTC CAAAGACGAG CCAGACAAAT 
GGTAGCCAAC AGAACGAATC CCCGCCAGCG GCGACCAGTG CAGACACTGC ACCCGCGAAT 
CCTTCACCAG CTCCCGCGGC CGCCGTTGCG TCCACATCCC AGGCGGCAAG TCCAACACAG 
ATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287508.1
CDS	358..12123
Translation	MCEVCADFQNLLELYEVRVASSDLKFQLLLKSEIETTFNYIQSWPQRQCMCLYRDTKNYDRFNLVVQSLICLTVQHLKHIDHLIDNYKRLTASAAQVVAQAQQQREQQRDEASAQAEAKESSAPAEEPKKEEPSGSAGEEAQGSGDGPAKKPPVGPCTPPPPQTANPQHKSHLQYTEEPWILPEVEKLLVLVSKVFLLNFPLYIAHKHGMHSRLDDLQAEEAHHLALICDLHDNDLPIYLLRNVSLFCNSGGFGAMSLCFEHPDLPVSTAHSMTAAVSNVKLWLNYHCNTQLFVPLRSRILQYMCKLSDQSLRSAATRAMADFVWSSMRDPLDVAVNFDTEGLALAFKYFTSTTLTMRLAGMAQINAHINLFNEICTTETVNEVELFGQRIANWLTENHIVQHLFGPNLHVEIVKQAHVLLNFLAVENQISEEDIKLIWQATQLKHCSKTIFDILPSLVKNLTPRPAMHLYSLLCRMDPKEHTEQSIYIASALTKQLWTRDTSRSQMNLMQDHLLGSNVTASSSDSGSIEGSNTEDDHVGADDSSIASGGGGGGVGNKSPIDGVTPCKQARHRRHICDPTTEKGKQISPEDMAKVDLVNKRIVNIIDNTSSEEELQSRAALELQLHRSRKKTSNKRRRQKTNKQIILPHELVEIWDGVEDVPSSDEADGEADGDGEGELLADSDECSDGATSQLVPDAVLKHLQGEGPFIRAIETNINELLSGAENDGSYSSPMSNKSEKNLADFDDEDVSPCEEELAQLVSSRANCSDVPPAFAAAAAAMMVAQSAMALQKSGESNVAAAAAAVAAAAAATGTAQQTLVAMNRQASVAAAAAVVAAAKAKSDSDVDLMDVVSGGKQHHSPKASQGSSTSGSTPVQPSFKLNDVCQPGNTLLWDLLQDDKIGQLGESLALEAEKALATLLCFSMDRQLRTKFIEGCLYNVANNRSVIVSLRLLPKLFASFQQFRPSDTHSMTMWAERNHRMMQCFFNNIRHYARRHAEVLITQNGEQQQQQLGGQLYSHKTQVSVRLQFLSSIFSTVGSPKSFRLTLEQLDALWEWLAHDPECADCYFSWLQAQAKGGDQHALGIEALQHLYLKKLPELRPEEFSMVALGLFQQLCSFARIAMAEYDNHSDQISASASAVGMYHLWKIALRAQSNDVSLAAIQYINMYYMGQQLRLEKEFVSQCMENLVQAATALESIDDENALMRVQRGLLLLNTHLDTFRRRYAFHLRRWAIEGKGIGSHSNLKNEGAGPPLRIVLQQAGLSEKSLLQMHACDLIADLKAEVSKWWESLQTGLAAPVLGLLLSDGPLRIITQGQELTSDYDERSLGDAGFKDNQIVYVSLGGRGARRKESNLEHPSMLPPPPKECLPTVLLLQPKYFEKLFCLMQTLGDMQPQASTVNPQHHTKAQLLSRRVWDILAMLPTNPHILDAFKSLVTDLSELEQLDAGGEEEQLATKRKQIKQKFRDLLDPNNLQKFMYSLHIVESLALTSSRRGESNGNVAMGNTPEQVRVKKSSMGRRRNSNEQPPPPPPEVKMSKEQLCELEAPLTPTPSTGLQDVETEASSSSGGDKENQPKQHSKRQKKGETFEQEKERPVGCSTPPSPTPPPPALSVVERGDNKWSEAFVKCGGLRHLYEIFSEGQLQQSAHPKELALNEWRHDCLASLLRILWLLGFEELQSADAHVLMSRPHPFMLQLMEVPQCLTRLSSILNDEVHQQHQASSANPLVFPYQFQHLRTGFWGRAQLIQFAMNILVSFVHASAEARRLLWAPTGTDHCRWLQKFILEDPEPAVRREICAGLYRICLGNAHSYRLLLAPLLHKLIALLPLAEQMSSGNQHTQFLLSEEGKDPYGPACRDYFWLLARLVDTLSPEMVAEEHIDIEMLCESISQSILTREYYELRHGYQDDGLVGLLNLMSNLIKYDTTFKYTPKALSFIEQLIGFLFDMPSPADRQKPKCKSASSRASAYDLLVELCRGCATNYAYLHGRLLAQHKSGPKQPYPWDYWPRDEGRAECGYVGLTNLGATCYMASCVQHLYMMPQARAAVLRVPPNAARKHGPTLLELQRMFAYLLESERKSYNPRSFCRVYQMDHQPLNTGEQKDMAEFFIDLVSKLEDMTPDLKHLVKRLFCGSLSNNVVSLDCGHVSRTAEDFYTVRCQVADMRNLQESLDEVTVKDTLEGDNMYTCSQCGKKVRAEKRACFKKLPQILCFNTMRYTFNMVTMLKEKVNTHFSFPLRLNMCHYVEKTLMPQQYKEERERRQKEKEGADGSGDGNDNEKAEATLDDDIEECYEYELVGVTVHTGTADGGHYYSFIKERTKTSYHTHERWFLFNDAEVKPFDPSQIAAECFGGEMTSKTYDSVTEKYLDFSFEKTNSAYMLFYERRLPEHLQRRHSELLVTPTPSPTVEEKSEAEEPTKMETSSSEIKADVDVEVEVEEKDKEKPAQTDTESKETPAKEEIADDKSKQDEPEEKKIEKQSREGEEKSETDEKPTEMTTVSTEEEKQPTANCDNHQQNNNSNSKASNDQQPSTSKAAQKLQLFRPLLNKELEDWIWQDNRQFLQDRNIFEHTYFNFMWQICGHIPQSLISETDVTCMAAKLSVSFFIETFIHAKEKPTMVPWVELLTKQFNASQEACEWFLSHMSQEPYWPVQVLIQCPNQMVRQMFQRLVIHVIQQLRASHAHLYLEVETDEDDKELIGQASCVTRFIGSLISLLEHGARANLRHLSEYFGLLCEFSRMGDEEAMYLLRIGVLKSLVDFYLGHKQTDSIDISSDNEDNSSEEALSVEKMRPASLDKMIALCASLVERSRGADFRLRLSPKDFSAIAGGKGFPFLYQQIKDGINPHQTKHLIHALCRWDERLATQIIGMLFASVTKHTELCAPFFKLLTLLTETQGGPVGLPCFTQLILPRMWDAAEYCPQSVLDWLSLQATKNKIAHAWILQSAEKWLEQFLLAHDNTRVRNAAAFLLVALVPSQPFRANFRAHSQHKLLALNPHSYRDINSDAQAVLHQVITLLLRLLRPARVYADIGAHGTTKLTAYFNLLSYCMVSKTEKLMIGSYMRSLWELFHPRLSEPSVPAHHNKHALLTFWHHSLVDCPENAAQVANCPEITRNIAFNYILADHDDAEIVTYNRSMLPAYYGLLRLCCEQSRALTRQLSQHQNLQWAFKNITPHPTQYAAAVDELFKLMALFATRHPDASEQEKLDVTQFRRAVIVSYTSSLDARVSWSTLISALKILVDNEEDRTMVIFNGGIEMCFEALHTLHSMHHEATACHVAGDLFDLLGEMLLLLATLRTRTDSPAQKKQQQQQQQLEQQLQLQQQQMLQKQQQQSQSQTQTPQSPQQKEKQLQQQMQQHLQLQQLQQMQFQQQHFLRQQHQHSALAKALPDAVKRLATLLNTFNSPEISRMALEVLKELVRNPSLETISILAPILINCHLSVANAPNAIGPLGPYFPRRGAKHTPWPLGAKNSPRPPRPMVQMCIALAELTPRGLDADYDVQVESFYRPYHDFIDVMMRMCVNTGTLNDTLVKLQCLVAIESTPLHFTYFPKFWVGIHNNALTHKYVELLVKNQLLVEYLHNVLRDERSMLKDACVREFLELYYHKVAAQLPVARMIYTINYGMHSKDDIDELCGDLFAIRIIAQATGVPASVRKELRGSLRALQNKSERFRKESERELSSSPYLPDPFPNKKQKRDSQKIKEKEHPQPESEKETSTENDKPSDVSMESSGNAEQATDSTKPPTPAGSDDEQMDKTSVPSSDDETELEDELQPTPKRNKKASNKKTAQDRVNEEREKRLITLITMESYIQSIFAILKRDASVPSSGATKEPSEEPCGEASTSAAAAVKLSRPKGACTPPEPITDVNDTRCNIDTETEAEADEQSPKTSQTNGSQQNESPPAATSADTAPANPSPAPAAAVASTSQAASPTQI

Target ORF information:

RefSeq Version	NM_001300579.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster puffyeye (puf), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300579.1

ORF Insert Sequence:

ATGTGCGAGG TGTGCGCCGA CTTCCAGAAT CTCCTGGAGC TCTATGAGGT GCGCGTGGCC 
AGCTCGGATC TGAAGTTCCA GCTGCTGCTG AAGAGCGAGA TCGAGACCAC CTTCAACTAC 
ATCCAGTCGT GGCCGCAGCG TCAGTGCATG TGCCTGTACC GGGACACCAA GAACTATGAC 
CGTTTCAACC TGGTAGTGCA GTCGCTCATC TGTCTGACGG TGCAGCATCT GAAGCACATC 
GACCACCTCA TCGACAACTA CAAGCGGCTA ACGGCCAGTG CTGCCCAGGT GGTGGCCCAG 
GCCCAGCAGC AGCGTGAACA GCAGCGGGAC GAAGCGTCAG CCCAGGCAGA GGCAAAGGAA 
TCATCCGCGC CAGCGGAAGA ACCTAAGAAG GAGGAGCCAT CTGGGTCCGC CGGTGAGGAG 
GCTCAGGGCT CCGGCGACGG GCCAGCCAAA AAGCCGCCAG TTGGCCCCTG CACTCCCCCT 
CCCCCGCAAA CGGCCAATCC TCAGCACAAA TCCCACCTGC AGTACACAGA GGAGCCCTGG 
ATCCTGCCCG AGGTGGAGAA ACTGTTGGTG CTCGTTTCAA AGGTGTTCCT GCTCAACTTC 
CCGCTGTACA TCGCCCACAA ACATGGCATG CACTCTCGGC TGGACGACCT GCAGGCTGAG 
GAGGCCCACC ACCTGGCCCT CATCTGCGAT CTTCACGACA ACGACCTGCC CATCTATCTG 
CTGCGGAATG TGAGCCTGTT CTGCAACTCG GGCGGCTTTG GCGCCATGTC GCTGTGCTTC 
GAACATCCCG ATCTGCCAGT GTCCACCGCT CACTCGATGA CCGCTGCCGT CTCAAATGTG 
AAACTTTGGC TGAACTATCA CTGTAATACT CAGCTCTTCG TGCCGCTGCG AAGTCGGATA 
CTGCAGTACA TGTGCAAGCT GTCGGATCAG AGCCTCCGAT CTGCCGCAAC CCGCGCGATG 
GCCGATTTTG TGTGGTCCTC TATGCGAGAC CCACTGGATG TTGCTGTCAA CTTCGACACC 
GAAGGATTGG CCCTGGCCTT TAAGTACTTC ACATCGACCA CGCTAACCAT GCGACTGGCA 
GGCATGGCCC AGATCAACGC ACACATTAAT CTATTTAACG AGATCTGCAC CACCGAAACG 
GTCAACGAAG TGGAGCTGTT CGGGCAGCGC ATCGCGAACT GGCTGACTGA AAATCATATT 
GTACAGCACC TGTTTGGCCC GAATCTGCAC GTAGAAATCG TTAAACAGGC ACACGTGCTG 
CTCAACTTTT TGGCGGTAGA GAACCAGATC TCTGAAGAGG ACATCAAGCT GATCTGGCAA 
GCCACCCAGC TGAAGCACTG CTCAAAGACG ATCTTCGACA TCCTGCCTTC GCTGGTGAAG 
AATCTGACCC CGCGCCCGGC AATGCACCTT TACTCCCTCC TTTGCCGCAT GGATCCCAAG 
GAGCACACTG AGCAGAGCAT ATACATAGCC TCAGCGCTAA CAAAACAGCT GTGGACTCGT 
GATACCTCAC GCAGTCAGAT GAACCTGATG CAAGACCACC TGCTGGGGTC CAACGTCACC 
GCTTCCAGCT CTGACAGCGG CAGCATTGAG GGCAGCAACA CAGAGGACGA CCACGTGGGC 
GCCGACGACA GTAGCATTGC CAGCGGTGGC GGCGGCGGAG GTGTTGGTAA CAAGAGTCCA 
ATCGATGGAG TTACTCCATG CAAGCAGGCC CGGCACAGGC GGCACATCTG TGACCCCACC 
ACCGAGAAGG GAAAGCAAAT AAGTCCCGAG GATATGGCCA AAGTAGACTT GGTGAACAAA 
CGGATTGTGA ACATTATCGA CAACACAAGC AGTGAAGAGG AGCTGCAATC GCGGGCCGCA 
CTGGAGCTGC AACTGCATCG CAGCCGCAAA AAGACGAGCA ACAAGCGACG GCGACAAAAA 
ACCAACAAAC AGATCATTCT GCCCCATGAG CTGGTCGAGA TTTGGGACGG AGTTGAAGAT 
GTGCCCAGCA GTGACGAGGC TGATGGCGAA GCCGATGGTG ATGGCGAGGG CGAACTTTTA 
GCGGACAGCG ATGAGTGCAG CGATGGTGCT ACGAGTCAGC TGGTACCCGA CGCCGTGCTA 
AAGCATCTGC AGGGAGAGGG ACCTTTTATC AGGGCCATCG AGACCAATAT CAATGAGCTG 
CTCAGTGGTG CCGAGAACGA TGGCAGTTAC TCATCCCCGA TGAGTAACAA ATCTGAAAAG 
AACCTGGCTG ACTTCGATGA CGAGGACGTG TCGCCTTGCG AGGAGGAGCT GGCCCAGCTG 
GTCAGCTCCC GCGCCAATTG TTCGGACGTT CCACCAGCCT TTGCAGCCGC TGCCGCCGCC 
ATGATGGTGG CCCAGTCGGC GATGGCCCTT CAGAAGTCTG GAGAGTCGAA TGTGGCTGCT 
GCTGCCGCTG CTGTGGCCGC TGCTGCAGCG GCTACCGGAA CTGCACAGCA AACGCTGGTG 
GCAATGAACC GGCAGGCCAG CGTTGCTGCA GCTGCCGCCG TGGTTGCTGC GGCCAAGGCA 
AAATCGGACT CAGATGTCGA TCTGATGGAT GTGGTTTCCG GCGGAAAGCA GCACCATTCA 
CCAAAGGCGA GCCAGGGCAG CTCTACGTCC GGGAGTACAC CGGTGCAGCC CTCGTTCAAG 
CTAAATGATG TCTGCCAGCC CGGAAACACA CTGCTTTGGG ATCTGCTTCA AGACGATAAA 
ATTGGCCAAC TAGGCGAGTC TCTGGCTCTA GAGGCGGAGA AAGCTCTGGC CACTCTCCTG 
TGCTTCAGCA TGGACCGCCA ACTGCGAACG AAATTCATCG AGGGTTGTCT GTATAACGTC 
GCCAACAACC GGAGTGTTAT TGTCAGTCTG CGACTGCTGC CCAAACTGTT CGCCTCGTTC 
CAACAGTTTC GACCCTCGGA CACGCACTCT ATGACGATGT GGGCAGAGCG AAATCACCGG 
ATGATGCAGT GCTTTTTCAA TAATATACGT CACTATGCGC GTCGCCATGC CGAGGTACTG 
ATCACCCAAA ACGGAGAGCA GCAGCAGCAG CAGCTGGGCG GACAACTCTA CTCGCACAAG 
ACACAAGTTT CTGTAAGACT GCAGTTCCTA TCATCGATAT TCTCGACGGT GGGATCCCCG 
AAAAGCTTCC GCCTAACGCT GGAGCAGCTT GACGCGCTAT GGGAGTGGCT GGCCCACGAT 
CCGGAGTGTG CCGACTGCTA CTTTTCGTGG CTGCAGGCCC AGGCCAAAGG CGGTGATCAG 
CATGCTCTGG GCATTGAAGC ACTTCAGCAT TTGTATTTGA AGAAACTCCC AGAGCTGCGA 
CCCGAGGAGT TCTCTATGGT TGCGTTGGGA CTATTTCAAC AGCTCTGTAG CTTCGCCCGC 
ATCGCCATGG CCGAGTACGA CAATCATAGC GACCAGATCT CGGCGAGCGC CAGTGCCGTT 
GGCATGTACC ATCTTTGGAA GATAGCGTTG CGTGCCCAGA GCAACGATGT CTCCTTGGCG 
GCCATACAGT ACATCAACAT GTACTACATG GGTCAACAAT TGCGCCTGGA GAAGGAGTTC 
GTTTCACAGT GTATGGAAAA CCTAGTTCAA GCGGCCACGG CTTTGGAAAG CATCGATGAT 
GAGAATGCTT TGATGCGTGT CCAGCGTGGC CTGCTCTTGC TGAACACCCA TTTGGATACG 
TTCCGGCGTC GATATGCGTT TCATCTGCGC CGCTGGGCCA TCGAAGGCAA AGGGATTGGA 
TCGCACAGTA ACCTTAAAAA TGAGGGAGCG GGTCCACCAC TACGAATAGT GCTGCAACAG 
GCAGGACTTT CGGAAAAAAG CCTTTTGCAG ATGCACGCGT GTGACTTAAT CGCCGACCTC 
AAGGCTGAGG TGTCCAAGTG GTGGGAGAGC CTTCAAACTG GACTAGCTGC TCCTGTGCTC 
GGTCTGCTGC TCAGCGATGG ACCGCTACGC ATTATCACAC AGGGACAGGA ATTGACCTCG 
GATTACGATG AACGGAGTCT CGGAGATGCT GGTTTCAAGG ATAACCAGAT CGTCTACGTA 
TCGTTAGGTG GACGTGGAGC CAGGCGAAAG GAATCCAATT TGGAGCATCC GTCAATGCTG 
CCGCCCCCAC CAAAGGAGTG TTTGCCCACG GTGCTCCTGC TGCAGCCAAA GTACTTCGAG 
AAGCTATTTT GCCTGATGCA GACCCTGGGC GATATGCAAC CGCAAGCGTC GACTGTAAAT 
CCCCAGCACC ACACAAAGGC GCAATTGCTC TCGCGACGCG TATGGGATAT CCTCGCCATG 
CTGCCAACTA ATCCGCACAT TCTAGACGCC TTTAAGAGTC TGGTTACGGA CCTAAGCGAA 
CTCGAGCAGC TTGATGCTGG CGGAGAGGAG GAGCAACTGG CCACCAAGCG CAAGCAAATC 
AAGCAGAAGT TCCGAGATCT TCTCGACCCG AACAACTTGC AAAAGTTCAT GTACTCCCTC 
CATATTGTGG AGAGTCTGGC CCTAACAAGT TCCAGACGTG GTGAATCCAA CGGCAATGTG 
GCCATGGGAA ATACACCGGA ACAGGTACGG GTGAAGAAAT CCTCGATGGG AAGACGGAGA 
AATAGCAACG AGCAGCCGCC ACCGCCGCCG CCAGAAGTCA AGATGAGCAA GGAGCAACTG 
TGCGAGCTGG AGGCCCCACT GACGCCCACA CCCTCGACTG GCTTGCAGGA TGTGGAAACG 
GAGGCCAGCA GTAGCAGCGG TGGCGACAAA GAAAACCAAC CGAAACAACA CAGCAAGCGT 
CAGAAGAAAG GCGAAACTTT TGAACAGGAA AAGGAGCGCC CAGTGGGTTG CTCCACGCCG 
CCGTCTCCAA CTCCTCCGCC ACCAGCACTT TCGGTAGTGG AAAGAGGTGA CAATAAGTGG 
AGTGAGGCGT TCGTCAAATG TGGTGGCCTT CGTCATCTGT ACGAAATCTT TAGTGAGGGC 
CAACTACAAC AAAGCGCACA TCCCAAGGAG TTGGCTTTAA ATGAATGGCG ACACGATTGC 
CTGGCCAGCC TGCTGCGCAT TCTCTGGCTT CTTGGATTCG AGGAACTGCA GTCGGCGGAT 
GCCCATGTTC TAATGTCACG TCCGCACCCG TTTATGCTAC AGTTGATGGA GGTACCACAG 
TGCCTGACCC GACTCTCATC GATCCTTAAC GACGAAGTTC ACCAGCAGCA TCAGGCTTCT 
TCGGCCAATC CACTGGTATT CCCCTATCAG TTCCAGCACT TGCGTACTGG CTTTTGGGGC 
AGGGCCCAGC TCATCCAGTT CGCTATGAAC ATTCTAGTGA GTTTTGTGCA CGCCTCAGCG 
GAAGCGAGAA GGCTTCTCTG GGCCCCAACT GGTACCGATC ACTGTCGATG GCTGCAGAAG 
TTCATCCTGG AGGATCCCGA ACCGGCGGTA CGGCGTGAGA TATGCGCTGG TCTTTATCGC 
ATTTGCTTAG GAAACGCACA TAGCTATCGC CTCTTACTAG CACCACTGCT GCACAAGCTC 
ATCGCCCTGT TGCCATTGGC CGAGCAGATG AGCTCTGGCA ACCAGCACAC GCAGTTTTTG 
CTCTCGGAAG AGGGCAAGGA TCCCTACGGA CCCGCCTGCC GTGACTACTT TTGGCTGCTG 
GCCAGATTGG TGGATACTCT CTCCCCGGAA ATGGTGGCCG AGGAGCACAT CGACATTGAA 
ATGCTGTGCG AAAGCATCTC GCAGAGCATT CTCACCCGAG AGTATTATGA ACTGCGCCAT 
GGTTACCAGG ACGATGGCTT GGTGGGTCTC CTAAATCTCA TGTCCAATTT GATCAAGTAC 
GACACCACAT TCAAGTACAC ACCCAAGGCG TTATCCTTTA TTGAGCAACT GATTGGCTTC 
CTGTTTGACA TGCCCTCGCC GGCAGATCGC CAGAAGCCCA AGTGCAAGTC GGCGTCGTCT 
CGAGCATCTG CCTACGATCT GCTGGTTGAA CTTTGCCGTG GATGCGCCAC AAACTATGCA 
TATCTGCATG GACGTCTGTT GGCACAGCAC AAATCCGGAC CCAAGCAGCC GTATCCCTGG 
GACTATTGGC CGCGCGATGA AGGCAGAGCT GAGTGCGGAT ATGTGGGGCT AACTAATTTG 
GGAGCCACTT GCTATATGGC CTCTTGCGTC CAGCACTTGT ACATGATGCC ACAGGCGCGA 
GCAGCTGTTT TGCGAGTTCC ACCCAACGCC GCCCGTAAGC ATGGGCCCAC ACTTCTGGAA 
CTACAACGGA TGTTTGCCTA CCTACTGGAG TCGGAACGGA AGTCTTACAA CCCGCGAAGC 
TTCTGCCGGG TGTACCAGAT GGATCACCAG CCGCTAAATA CGGGAGAGCA AAAGGATATG 
GCAGAGTTCT TTATCGATTT GGTGTCCAAA CTGGAAGATA TGACGCCTGA CTTGAAGCAC 
CTGGTGAAAA GGCTCTTCTG TGGATCCTTG AGCAATAATG TCGTCTCCCT GGATTGCGGT 
CACGTTTCCC GCACGGCCGA GGACTTTTAC ACGGTTCGCT GCCAGGTTGC AGATATGCGC 
AATCTGCAGG AATCCCTGGA CGAAGTCACC GTTAAGGATA CCCTCGAGGG CGACAACATG 
TACACCTGCT CGCAGTGCGG CAAAAAAGTA CGAGCCGAGA AGCGGGCTTG CTTCAAGAAG 
CTTCCCCAGA TCTTGTGCTT CAACACCATG CGCTACACCT TCAATATGGT CACCATGCTC 
AAGGAGAAGG TCAACACGCA CTTTTCCTTT CCGCTACGCC TGAACATGTG TCACTATGTG 
GAGAAGACCT TGATGCCGCA GCAGTACAAA GAGGAGCGAG AAAGGCGCCA AAAGGAGAAG 
GAAGGAGCAG ATGGTAGTGG CGATGGGAAT GACAACGAAA AGGCAGAGGC CACGCTAGAT 
GACGATATTG AAGAATGCTA CGAGTACGAA CTGGTGGGCG TCACTGTCCA CACGGGCACA 
GCGGATGGCG GCCACTACTA CAGCTTTATA AAGGAGCGGA CGAAGACTAG TTACCATACG 
CACGAGCGGT GGTTTCTTTT CAACGATGCC GAAGTGAAGC CTTTCGATCC CTCACAGATA 
GCGGCGGAGT GCTTTGGCGG CGAGATGACG AGCAAAACCT ACGACTCAGT GACGGAGAAA 
TATCTGGACT TTAGTTTCGA GAAGACCAAC TCTGCCTACA TGCTGTTCTA CGAGCGCCGA 
CTGCCAGAGC ATCTGCAGCG CCGGCATTCC GAGCTGCTGG TTACACCCAC GCCCAGTCCC 
ACAGTGGAAG AGAAGTCCGA GGCGGAGGAG CCCACTAAAA TGGAGACAAG CTCTAGCGAA 
ATCAAGGCAG ATGTGGATGT GGAAGTGGAA GTTGAGGAGA AGGACAAGGA GAAGCCTGCG 
CAGACTGACA CAGAATCTAA GGAAACGCCT GCAAAAGAGG AGATCGCGGA TGACAAATCG 
AAGCAGGATG AGCCTGAGGA AAAGAAAATC GAAAAGCAAT CCAGAGAGGG CGAAGAGAAA 
TCGGAAACGG ACGAAAAGCC CACGGAGATG ACGACCGTGA GCACTGAGGA GGAAAAGCAG 
CCAACAGCCA ATTGTGATAA TCACCAGCAA AACAACAATA GCAACAGCAA AGCCAGCAAC 
GATCAGCAAC CATCCACGTC GAAGGCTGCC CAGAAGCTAC AACTATTTCG TCCATTGTTG 
AACAAGGAGC TCGAGGATTG GATTTGGCAG GACAATCGAC AGTTCCTGCA GGATCGCAAC 
ATTTTCGAGC ACACATATTT CAACTTTATG TGGCAGATAT GTGGGCACAT ACCCCAATCA 
TTAATATCAG AGACGGACGT CACCTGCATG GCAGCCAAGT TGTCTGTGTC CTTTTTCATT 
GAGACTTTTA TTCATGCTAA GGAAAAGCCC ACTATGGTGC CCTGGGTGGA GCTGCTGACC 
AAGCAGTTCA ATGCCAGCCA AGAGGCCTGC GAGTGGTTTC TGTCGCACAT GTCGCAGGAG 
CCCTACTGGC CGGTCCAGGT GCTAATCCAG TGTCCCAACC AAATGGTGCG TCAAATGTTT 
CAGCGTTTGG TAATCCATGT CATCCAGCAA CTACGTGCCT CACATGCTCA TCTGTATCTG 
GAAGTGGAGA CAGACGAGGA TGACAAGGAG TTAATAGGCC AAGCCTCTTG CGTCACCCGT 
TTCATTGGTT CGCTCATCTC GCTCCTGGAG CACGGTGCCC GAGCGAACCT GCGGCACTTG 
TCCGAGTACT TTGGACTGTT GTGCGAGTTC TCACGGATGG GAGATGAGGA AGCCATGTAC 
TTGCTGCGCA TCGGTGTACT CAAGAGCCTG GTCGACTTCT ATCTGGGTCA CAAGCAAACA 
GACAGCATAG ACATAAGCTC TGACAACGAA GACAACTCCT CTGAAGAGGC GCTGTCCGTG 
GAGAAGATGC GTCCGGCCTC GCTGGACAAG ATGATTGCTC TGTGCGCCAG CCTAGTGGAA 
AGATCAAGAG GAGCCGACTT CCGGCTGCGA CTCTCGCCCA AAGACTTCTC TGCAATCGCC 
GGCGGCAAGG GTTTTCCATT TTTGTACCAA CAAATAAAGG ACGGCATTAA TCCGCACCAG 
ACAAAGCATT TAATACACGC GCTTTGTCGC TGGGATGAGC GCCTGGCCAC GCAGATCATT 
GGCATGCTCT TCGCGTCGGT AACCAAGCAC ACGGAGCTGT GCGCGCCATT CTTCAAGCTG 
CTAACTCTGC TCACCGAGAC GCAGGGCGGT CCAGTGGGCC TGCCTTGTTT CACCCAGTTG 
ATCCTGCCAC GAATGTGGGA CGCCGCCGAG TACTGTCCAC AGTCGGTGCT CGACTGGCTC 
TCGCTGCAGG CCACCAAGAA CAAGATCGCC CACGCCTGGA TACTTCAATC GGCCGAGAAG 
TGGCTCGAGC AATTCCTGCT GGCCCACGAT AACACTCGTG TTCGGAACGC TGCCGCCTTT 
CTGCTGGTGG CACTGGTACC CTCCCAGCCA TTCCGGGCCA ACTTCCGCGC CCACTCGCAA 
CACAAGCTGC TGGCCCTCAA TCCGCACAGT TATCGCGACA TAAATAGCGA TGCCCAGGCG 
GTGCTGCACC AGGTGATCAC GCTGCTCCTG CGATTGCTGC GTCCAGCGCG CGTCTATGCG 
GATATCGGAG CCCATGGCAC GACCAAGCTG ACCGCGTACT TTAATCTGCT CAGCTATTGT 
ATGGTTTCAA AGACTGAAAA ACTAATGATA GGTTCGTACA TGCGCTCCCT GTGGGAGCTG 
TTCCATCCAC GCCTCTCGGA GCCCAGTGTT CCGGCCCATC ACAATAAGCA CGCACTATTA 
ACCTTCTGGC ACCACTCACT GGTGGACTGT CCGGAGAATG CTGCCCAGGT GGCCAACTGC 
CCGGAGATTA CGCGCAACAT TGCATTTAAC TATATACTGG CCGATCATGA TGACGCGGAG 
ATAGTCACCT ACAACCGTTC CATGCTGCCC GCCTATTATG GACTACTTCG TCTGTGCTGC 
GAGCAGAGCC GTGCACTCAC ACGCCAATTG TCCCAGCATC AGAATCTTCA GTGGGCATTT 
AAAAACATCA CACCGCATCC CACGCAGTAC GCGGCCGCCG TGGACGAGCT TTTCAAGTTG 
ATGGCTCTGT TCGCCACCCG TCATCCGGAT GCTAGTGAAC AGGAAAAGCT TGATGTGACC 
CAGTTTAGGA GGGCTGTGAT TGTGTCCTAC ACCAGTAGTC TGGATGCACG TGTCTCTTGG 
TCCACGCTGA TCAGTGCTCT TAAGATACTC GTGGACAATG AGGAGGACCG CACGATGGTG 
ATCTTCAACG GCGGCATTGA GATGTGTTTC GAGGCGCTGC ACACGCTACA TTCCATGCAC 
CACGAGGCCA CCGCCTGCCA CGTGGCCGGA GACCTATTCG ATCTCTTGGG CGAGATGCTA 
CTCCTCCTAG CCACTCTTCG AACCCGCACA GACAGTCCTG CTCAAAAGAA GCAGCAGCAA 
CAACAGCAGC AGCTGGAGCA ACAGTTGCAG TTGCAGCAGC AACAGATGCT GCAGAAACAA 
CAGCAGCAGT CGCAAAGCCA AACGCAAACA CCGCAGTCGC CGCAGCAAAA GGAAAAGCAG 
CTGCAGCAAC AAATGCAGCA GCATCTGCAG CTGCAACAGT TGCAGCAAAT GCAGTTCCAG 
CAGCAACACT TCCTGCGCCA GCAACATCAA CACTCGGCGC TGGCAAAGGC ACTGCCGGAT 
GCAGTGAAGC GACTGGCTAC GCTACTCAAC ACCTTCAATT CGCCAGAGAT CAGTCGCATG 
GCCTTGGAGG TGCTCAAGGA GCTGGTGCGC AATCCCAGCC TGGAAACCAT TAGTATTCTG 
GCGCCCATTC TTATCAACTG TCATCTGTCC GTGGCGAATG CTCCCAATGC GATCGGTCCT 
CTGGGACCGT ACTTTCCGCG TCGCGGTGCG AAGCATACGC CTTGGCCATT GGGAGCCAAG 
AACTCACCCC GTCCACCGCG GCCCATGGTA CAGATGTGCA TCGCTCTGGC TGAGCTGACA 
CCACGCGGTC TCGATGCCGA TTACGACGTT CAGGTGGAGT CCTTTTACCG ACCCTACCAT 
GACTTTATCG ATGTCATGAT GCGCATGTGC GTCAACACGG GCACTCTGAA CGACACGCTA 
GTCAAGCTGC AATGCTTGGT GGCCATCGAG TCCACGCCGC TGCACTTCAC CTATTTCCCC 
AAATTCTGGG TGGGCATACA CAACAATGCT CTGACGCACA AATACGTTGA GCTGCTGGTG 
AAAAATCAAC TGTTGGTAGA GTACCTTCAC AATGTCCTTA GGGACGAGCG AAGCATGTTG 
AAGGATGCCT GCGTTCGTGA ATTTCTGGAG CTCTATTATC ACAAGGTTGC CGCCCAGCTG 
CCAGTGGCCC GTATGATCTA CACAATCAAC TATGGTATGC ACTCCAAGGA CGACATTGAT 
GAGCTGTGTG GCGATCTGTT CGCCATCCGA ATCATTGCCC AGGCCACTGG AGTGCCGGCC 
TCAGTGCGCA AGGAGTTGCG AGGATCGCTG AGGGCGCTTC AGAACAAGAG CGAGCGGTTT 
AGGAAGGAAT CGGAGAGGGA GCTATCATCA TCTCCCTATT TGCCAGATCC ATTTCCGAAC 
AAGAAGCAGA AACGTGACTC GCAAAAGATC AAGGAAAAGG AGCACCCTCA GCCGGAGAGC 
GAGAAAGAGA CCTCAACGGA GAACGACAAA CCATCAGATG TTTCCATGGA ATCCAGCGGT 
AACGCGGAGC AAGCAACGGA CAGCACAAAA CCACCAACTC CTGCAGGCAG CGACGATGAG 
CAGATGGACA AAACTTCAGT TCCCTCGTCC GATGACGAGA CAGAGTTGGA GGACGAGCTG 
CAGCCGACGC CCAAGCGCAA CAAGAAGGCT TCGAATAAGA AGACCGCCCA GGATCGTGTC 
AACGAGGAGC GCGAGAAGCG ACTTATCACG CTGATCACCA TGGAATCATA TATCCAGAGC 
ATTTTTGCCA TCCTTAAGAG AGATGCCAGT GTCCCCAGTT CGGGAGCGAC TAAGGAGCCC 
AGTGAGGAAC CGTGTGGCGA GGCATCCACT TCGGCGGCTG CCGCTGTAAA GTTATCTCGT 
CCCAAAGGAG CCTGTACTCC GCCGGAGCCA ATTACCGACG TAAACGATAC GCGTTGCAAC 
ATAGATACTG AGACAGAGGC GGAGGCGGAT GAGCAATCTC CAAAGACGAG CCAGACAAAT 
GGTAGCCAAC AGAACGAATC CCCGCCAGCG GCGACCAGTG CAGACACTGC ACCCGCGAAT 
CCTTCACCAG CTCCCGCGGC CGCCGTTGCG TCCACATCCC AGGCGGCAAG TCCAACACAG 
ATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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puf cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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