Pp1alpha-96A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pp1alpha-96A
Entrez Gene ID	42922
Full Name	Protein phosphatase 1alpha at 96A
Synonyms	96A,CG6593,DmPp1-96A,Dmel\CG6593,PP1,PP1 96A,PP1 alpha96a,PP1alpha,PP1alpha96A,PP1alpha[[1]],PP1c,PP1c 96A,Pp1-96A,pp1alpha96A
General protein information	Preferred Names Protein phosphatase 1alpha at 96A Names CG6593 gene product from transcript CG6593-RB CG6593-PA CG6593-PB Pp1alpha-96A-PA Pp1alpha-96A-PB protein phosphatase 1 protein phosphatase 1alpha at 96A
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96A5-96A5

mRNA and Protein(s)

mRNA	Protein	Name
NM_079760.3	NP_524484.1	protein phosphatase 1alpha at 96A, isoform A
NM_001275990.1	NP_001262919.1	protein phosphatase 1alpha at 96A, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	PPP1CC	NP_002701.1
Bos taurus (cattle)	PPP1CC	NP_777006.1
Mus musculus (house mouse)	Gm5601	XP_006508478.1
Oryza sativa (rice)	Os03g0268000	NP_001049669.1
Xenopus tropicalis (tropical clawed frog)	ppp1cc	NP_998835.1
Canis lupus familiaris (dog)	PPP1CC	NP_001003033.1
Drosophila melanogaster (fruit fly)	Pp1alpha-96A	NP_001262919.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011166	XP_309483.4
	PPP1CC	XP_001108424.1
Danio rerio (zebrafish)	ppp1cc	XP_700468.5
Gallus gallus (chicken)	PPP1CC	NP_001006190.1
Schizosaccharomyces pombe (fission yeast)	sds21	NP_587898.1
Pan troglodytes (chimpanzee)	PPP1CC	XP_001144540.2
Drosophila melanogaster (fruit fly)	Pp1-87B	NP_524937.1
Drosophila melanogaster (fruit fly)	Pp1-13C	NP_524921.1
Arabidopsis thaliana (thale cress)	TOPP4	NP_181514.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Overexpression of the PP1 alpha isozymes does not rescue semilethal PP1 beta 9C mutants, whereas overexpression of either PP1 alpha 96A or PP1 beta 9C does rescue a lethal PP1 alpha 87B mutant combination.
Title: Essential, overlapping and redundant roles of the Drosophila protein phosphatase 1 alpha and 1 beta genes.

The following Pp1alpha-96A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pp1alpha-96A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05963	NM_079760.3 Latest version!	Drosophila melanogaster protein phosphatase 1alpha at 96A, transcript variant A (Pp1alpha-96A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa05963	NM_001275990.1 Latest version!	Drosophila melanogaster protein phosphatase 1alpha at 96A, transcript variant B (Pp1alpha-96A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pp1alpha-96A ( NM_079760.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524484.1 Drosophila melanogaster protein phosphatase 1alpha at 96A, transcript variant A (Pp1alpha-96A), mRNA.

Price & Availability↑

CloneID	OFa05963
Clone ID Related Accession (Same CDS sequence)	NM_079760.3 , NM_001275990.1
Accession Version	NM_079760.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein phosphatase 1alpha at 96A, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079760.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGATA TCATGAACAT CGACAGTATC ATCTCGCGCC TGCTGGAGGT GCGCGGTGCA 
CGACCGGGAA AGAATGTTCA GCTATCAGAG AGCGAGATCC GCAGCCTGTG CCTCAAGTCC 
CGCGAGATAT TCCTGTCGCA GCCCATACTC CTGGAGCTGG AGGCACCGCT GAAGATCTGC 
GGCGACATAC ACGGACAATA TTATGACCTC TTGCGCCTGT TCGAATACGG CGGCTTTCCG 
CCCGAGTCAA ACTACCTGTT CCTGGGCGAC TATGTCGATC GCGGCAAGCA ATCCCTGGAG 
ACGATATGCC TGCTGCTGGC CTACAAGATC AAGTACGCGG AGAACTTCTT CTTGCTGCGC 
GGCAACCATG AGTGCGCCAG CATCAATCGC ATCTACGGGT TCTATGACGA ATGTAAACGT 
CGCTACACCA TCAAGCTGTG GAAAACTTTC ACGGACTGCT TCAACTGCCT GCCGGTGGCC 
GCCATCGTGG ATGAGAAGAT CTTCTGCTGC CACGGTGGCC TGAGTCCAGA TCTGTCGTCC 
ATGGAGCAGA TACGACGCAT CATGCGTCCC ACAGACGTGC CCGATCAGGG ACTGCTGTGC 
GATCTGCTGT GGTCGGATCC GGATAAGGAT ACCATGGGCT GGGGCGAGAA TGATCGCGGC 
GTGAGCTTCA CATTTGGAGC AGAGGTTGTG GGTAAATTTT TACAGAAGCA CGAATTTGAC 
CTGATCTGTC GGGCTCATCA GGTAGTGGAG GACGGTTATG AGTTCTTCGC CAAACGTCAG 
CTGGTGACGC TCTTCTCGGC GCCCAACTAC TGCGGGGAAT TCGACAACGC GGGTGCCATG 
ATGTCCGTGG ACGACACATT GATGTGCTCC TTCCAGATAT TGAAACCCGC CGACAAGCGA 
CGATTTGTTT ATCCCAACTT TGGCAGCTCA GGACGTCCTT TGACACCGCC ACGCGGAGCT 
AACAATAAAA ACAAGAAAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524484.1
CDS	390..1373
Translation	MSDIMNIDSIISRLLEVRGARPGKNVQLSESEIRSLCLKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYAENFFLLRGNHECASINRIYGFYDECKRRYTIKLWKTFTDCFNCLPVAAIVDEKIFCCHGGLSPDLSSMEQIRRIMRPTDVPDQGLLCDLLWSDPDKDTMGWGENDRGVSFTFGAEVVGKFLQKHEFDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDDTLMCSFQILKPADKRRFVYPNFGSSGRPLTPPRGANNKNKKK

Target ORF information:

RefSeq Version	NM_079760.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein phosphatase 1alpha at 96A, transcript variant A (Pp1alpha-96A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079760.3

ORF Insert Sequence:

ATGTCGGATA TCATGAACAT CGACAGTATC ATCTCGCGCC TGCTGGAGGT GCGCGGTGCA 
CGACCGGGAA AGAATGTTCA GCTATCAGAG AGCGAGATCC GCAGCCTGTG CCTCAAGTCC 
CGCGAGATAT TCCTGTCGCA GCCCATACTC CTGGAGCTGG AGGCACCGCT GAAGATCTGC 
GGCGACATAC ACGGACAATA TTATGACCTC TTGCGCCTGT TCGAATACGG CGGCTTTCCG 
CCCGAGTCAA ACTACCTGTT CCTGGGCGAC TATGTCGATC GCGGCAAGCA ATCCCTGGAG 
ACGATATGCC TGCTGCTGGC CTACAAGATC AAGTACGCGG AGAACTTCTT CTTGCTGCGC 
GGCAACCATG AGTGCGCCAG CATCAATCGC ATCTACGGGT TCTATGACGA ATGTAAACGT 
CGCTACACCA TCAAGCTGTG GAAAACTTTC ACGGACTGCT TCAACTGCCT GCCGGTGGCC 
GCCATCGTGG ATGAGAAGAT CTTCTGCTGC CACGGTGGCC TGAGTCCAGA TCTGTCGTCC 
ATGGAGCAGA TACGACGCAT CATGCGTCCC ACAGACGTGC CCGATCAGGG ACTGCTGTGC 
GATCTGCTGT GGTCGGATCC GGATAAGGAT ACCATGGGCT GGGGCGAGAA TGATCGCGGC 
GTGAGCTTCA CATTTGGAGC AGAGGTTGTG GGTAAATTTT TACAGAAGCA CGAATTTGAC 
CTGATCTGTC GGGCTCATCA GGTAGTGGAG GACGGTTATG AGTTCTTCGC CAAACGTCAG 
CTGGTGACGC TCTTCTCGGC GCCCAACTAC TGCGGGGAAT TCGACAACGC GGGTGCCATG 
ATGTCCGTGG ACGACACATT GATGTGCTCC TTCCAGATAT TGAAACCCGC CGACAAGCGA 
CGATTTGTTT ATCCCAACTT TGGCAGCTCA GGACGTCCTT TGACACCGCC ACGCGGAGCT 
AACAATAAAA ACAAGAAAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pp1alpha-96A ( NM_001275990.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262919.1 Drosophila melanogaster protein phosphatase 1alpha at 96A, transcript variant B (Pp1alpha-96A), mRNA.

Price & Availability↑

CloneID	OFa05963
Clone ID Related Accession (Same CDS sequence)	NM_079760.3 , NM_001275990.1
Accession Version	NM_001275990.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein phosphatase 1alpha at 96A, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGATA TCATGAACAT CGACAGTATC ATCTCGCGCC TGCTGGAGGT GCGCGGTGCA 
CGACCGGGAA AGAATGTTCA GCTATCAGAG AGCGAGATCC GCAGCCTGTG CCTCAAGTCC 
CGCGAGATAT TCCTGTCGCA GCCCATACTC CTGGAGCTGG AGGCACCGCT GAAGATCTGC 
GGCGACATAC ACGGACAATA TTATGACCTC TTGCGCCTGT TCGAATACGG CGGCTTTCCG 
CCCGAGTCAA ACTACCTGTT CCTGGGCGAC TATGTCGATC GCGGCAAGCA ATCCCTGGAG 
ACGATATGCC TGCTGCTGGC CTACAAGATC AAGTACGCGG AGAACTTCTT CTTGCTGCGC 
GGCAACCATG AGTGCGCCAG CATCAATCGC ATCTACGGGT TCTATGACGA ATGTAAACGT 
CGCTACACCA TCAAGCTGTG GAAAACTTTC ACGGACTGCT TCAACTGCCT GCCGGTGGCC 
GCCATCGTGG ATGAGAAGAT CTTCTGCTGC CACGGTGGCC TGAGTCCAGA TCTGTCGTCC 
ATGGAGCAGA TACGACGCAT CATGCGTCCC ACAGACGTGC CCGATCAGGG ACTGCTGTGC 
GATCTGCTGT GGTCGGATCC GGATAAGGAT ACCATGGGCT GGGGCGAGAA TGATCGCGGC 
GTGAGCTTCA CATTTGGAGC AGAGGTTGTG GGTAAATTTT TACAGAAGCA CGAATTTGAC 
CTGATCTGTC GGGCTCATCA GGTAGTGGAG GACGGTTATG AGTTCTTCGC CAAACGTCAG 
CTGGTGACGC TCTTCTCGGC GCCCAACTAC TGCGGGGAAT TCGACAACGC GGGTGCCATG 
ATGTCCGTGG ACGACACATT GATGTGCTCC TTCCAGATAT TGAAACCCGC CGACAAGCGA 
CGATTTGTTT ATCCCAACTT TGGCAGCTCA GGACGTCCTT TGACACCGCC ACGCGGAGCT 
AACAATAAAA ACAAGAAAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262919.1
CDS	390..1373
Translation	MSDIMNIDSIISRLLEVRGARPGKNVQLSESEIRSLCLKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLLLAYKIKYAENFFLLRGNHECASINRIYGFYDECKRRYTIKLWKTFTDCFNCLPVAAIVDEKIFCCHGGLSPDLSSMEQIRRIMRPTDVPDQGLLCDLLWSDPDKDTMGWGENDRGVSFTFGAEVVGKFLQKHEFDLICRAHQVVEDGYEFFAKRQLVTLFSAPNYCGEFDNAGAMMSVDDTLMCSFQILKPADKRRFVYPNFGSSGRPLTPPRGANNKNKKK

Target ORF information:

RefSeq Version	NM_001275990.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein phosphatase 1alpha at 96A, transcript variant B (Pp1alpha-96A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275990.1

ORF Insert Sequence:

ATGTCGGATA TCATGAACAT CGACAGTATC ATCTCGCGCC TGCTGGAGGT GCGCGGTGCA 
CGACCGGGAA AGAATGTTCA GCTATCAGAG AGCGAGATCC GCAGCCTGTG CCTCAAGTCC 
CGCGAGATAT TCCTGTCGCA GCCCATACTC CTGGAGCTGG AGGCACCGCT GAAGATCTGC 
GGCGACATAC ACGGACAATA TTATGACCTC TTGCGCCTGT TCGAATACGG CGGCTTTCCG 
CCCGAGTCAA ACTACCTGTT CCTGGGCGAC TATGTCGATC GCGGCAAGCA ATCCCTGGAG 
ACGATATGCC TGCTGCTGGC CTACAAGATC AAGTACGCGG AGAACTTCTT CTTGCTGCGC 
GGCAACCATG AGTGCGCCAG CATCAATCGC ATCTACGGGT TCTATGACGA ATGTAAACGT 
CGCTACACCA TCAAGCTGTG GAAAACTTTC ACGGACTGCT TCAACTGCCT GCCGGTGGCC 
GCCATCGTGG ATGAGAAGAT CTTCTGCTGC CACGGTGGCC TGAGTCCAGA TCTGTCGTCC 
ATGGAGCAGA TACGACGCAT CATGCGTCCC ACAGACGTGC CCGATCAGGG ACTGCTGTGC 
GATCTGCTGT GGTCGGATCC GGATAAGGAT ACCATGGGCT GGGGCGAGAA TGATCGCGGC 
GTGAGCTTCA CATTTGGAGC AGAGGTTGTG GGTAAATTTT TACAGAAGCA CGAATTTGAC 
CTGATCTGTC GGGCTCATCA GGTAGTGGAG GACGGTTATG AGTTCTTCGC CAAACGTCAG 
CTGGTGACGC TCTTCTCGGC GCCCAACTAC TGCGGGGAAT TCGACAACGC GGGTGCCATG 
ATGTCCGTGG ACGACACATT GATGTGCTCC TTCCAGATAT TGAAACCCGC CGACAAGCGA 
CGATTTGTTT ATCCCAACTT TGGCAGCTCA GGACGTCCTT TGACACCGCC ACGCGGAGCT 
AACAATAAAA ACAAGAAAAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pp1alpha-96A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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