crb cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following crb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa05934 NM_001043286.2
Latest version!
Drosophila melanogaster crumbs (crb), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OFa05935 NM_079756.3
Latest version!
Drosophila melanogaster crumbs (crb), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OFa08721 NM_001260355.2
Latest version!
Drosophila melanogaster crumbs (crb), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OFa08722 NM_001275980.1
Latest version!
Drosophila melanogaster crumbs (crb), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa05934
    Clone ID Related Accession (Same CDS sequence) NM_001043286.2
    Accession Version NM_001043286.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6570bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product crumbs, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_001043286.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCTAAAA TCGCCAATGC GTCACTGTCG CAACAACAAA AACAGCGGCA AGCAGAAACA 
    GCAACGACCA CAACAACAAC AGTGGCGGCA TCAGTAGAAA CAGCAACAAC AACAGCAAGA
    AGTCGAGACC GCACGAAAAG CGCAGCTCAA ATTACGTCGC ATTTGCTCAA GCGCGCCATT
    TCAGTTTATT CGTCGCCTCA ATGGATACCG CTCTTCATAC TTATTTACTT AGCAACAGAT
    GTCGCCTCAG TGGCGGTGCC GACGAAGGAG GCGTACTTTA ATGGCTCCAC TTACCTCCGC
    CTCACCACGC CGATGCCCAT TTGGGATCAC TCGGCGATTA GTTTCCGCTC GTGCCGCGGC
    GGCGAGATCC TCGCCCAGCA GTACAACAAG AACTCCATTG TAATCTCAGT GCTCAATGAC
    TTTCTGCAAA TCTCACTGGC TGGACCCGCC GTCCATGGGC CCAACAACCG GCTGGATGTC
    AAGCTGCCCT ACCAACTGCT GGACAACCGC TGGCATACGC TGCAGTTCAA GTACGAGTAC
    GGAAATCTCT ACCTGCATGT GGATCGCGCG GCAAGCATAT TTGCCAACTC CACGTACAAC
    AGTCAGTTCC TGACTAACCA GGACATTGGC TACAAGGACG CCATCTTGAT ACTGGGAAAC
    TCCTTCTCCG GTTGCCTTCT GGATGGACCA GGTCTGCAAT TTGTGAACAA CTCGACGGTG
    CAGAATGTGG TCTTTGGTCA CTGTCCCTTG ACCCCGGGTC CCTGCAGCGA TCACGATCTT
    TTCACTCGAC TGCCAGACAA CTTTTGTCTG AATGATCCCT GTATGGGCCA TGGAACCTGC
    TCGTCCAGTC CAGAGGGATA CGAATGTCGG TGCACGGCAC GTTACTCGGG AAAAAATTGC
    CAGAAGGACA ATGGCTCGCC GTGTGCCAAA AATCCATGTG AGAACGGTGG TTCCTGCCTG
    GAGAATTCCC GAGGGGATTA CCAGTGCTTC TGTGATCCCA ACCACAGTGG TCAGCACTGC
    GAGACGGAGG TGAACATCCA CCCACTCTGC CAGACGAACC CCTGCCTGAA CAATGGAGCA
    TGTGTGGTGA TTGGCGGCAG TGGAGCACTC ACCTGCGAGT GTCCCAAAGG ATACGCTGGT
    GCCAGGTGCG AGGTGGACAC GGATGAGTGC GCCTCACAGC CGTGTCAGAA CAACGGAAGC
    TGCATAGACC GTATCAATGG ATTCAGCTGC GATTGCAGCG GCACCGGTTA CACGGGTGCG
    TTCTGCCAGA CGAATGTGGA TGAGTGCGAC AAGAATCCGT GCCTGAACGG CGGCAGATGC
    TTCGATACCT ACGGATGGTA CACCTGCCAG TGTCTGGATG GTTGGGGCGG CGAGATTTGT
    GATCGACCCA TGACATGCCA GACGCAGCAG TGTTTGAACG GTGGCACCTG CCTGGACAAG
    CCCATCGGCT TCCAGTGCCT CTGTCCGCCG GAGTACACTG GTGAACTGTG CCAAATCGCA
    CCCAGTTGCG CACAGCAGTG CCCCATCGAT TCGGAGTGCG TAGGCGGCAA ATGCGTTTGT
    AAGCCAGGCT CATCGGCTTC CGACATGGAG CCGCTGACTC CGCTCGAGCT GGACATTCTC
    GACGCCACGC TGTGCCCCAG CGAAAAGAAG AAGCGCTACA TATCGCCCGA GTGGCTCAAA
    CGCAAGCGTT GTGAGCTAAA ACTAAGTTAC AACTGTCAAA CGAGCACGGG CGATGGAGCT
    TCTGCTTTGG CCCTGACACC CATCAACTGC AATGCCACCA ATGGAAAGTG TCTCAATGGA
    GGAACTTGCT CCATGAACGG AACCCACTGC TATTGCGCCG TCGGCTATTC GGGAGATCGT
    TGCGAGAAGG CCGAGAACTG TTCGCCACTG AACTGCCAGG AACCGATGGT ATGTGTCCAG
    AATCAGTGCC TCTGCCCGGA GAACAAGGTG TGCAACCAGT GCGCCACACA ACCGTGCCAG
    AATGGCGGTG AATGTGTGGA CCTACCGAAT GGAGACTACG AGTGCAAGTG CACCCGTGGA
    TGGACTGGAC GCACCTGTGG CAACGACGTG GACGAGTGCA CCCTGCATCC GAAGATCTGC
    GGCAATGGTA TCTGCAAGAA CGAGAAGGGA TCGTACAAAT GCTACTGTAC GCCTGGATTT
    ACCGGAGTCC ACTGCGATTC CGATGTGGAC GAATGCCTTA GCTTTCCCTG CCTCAACGGA
    GCCACGTGCC ACAACAAGAT AAATGCCTAC GAGTGCGTTT GCCAACCAGG ATATGAGGGA
    GAGAACTGCG AAGTAGATAT CGATGAGTGC GGCAGTAATC CCTGCTCGAA CGGATCCACC
    TGCATCGACA GGATCAACAA CTTCACCTGC AACTGCATTC CGGGAATGAC GGGCCGCATA
    TGTGACATCG ACATTGACGA CTGTGTGGGA GATCCCTGCT TGAATGGTGG TCAGTGCATC
    GACCAATTGG GTGGCTTCCG TTGTGACTGC AGTGGCACCG GTTATGAGGG CGAGAATTGT
    GAGCTGAACA TAGACGAATG CCTCTCGAAT CCGTGCACGA ATGGTGCCAA GTGCCTGGAC
    AGGGTTAAGG ACTACTTCTG CGATTGCCAC AACGGCTACA AGGGAAAGAA CTGTGAGCAG
    GACATCAACG AGTGCGAGAG TAATCCCTGC CAGTACAATG GCAACTGCCT AGAGCGTTCG
    AACATAACGC TTTACCAAAT GAGTAGGATC ACGGATCTGC CCAAGGTGTT TAGCCAGCCC
    TTTAGCTTTG AGAATGCCAG CGGCTACGAG TGCGTCTGTG TGCCCGGCAT CATTGGAAAG
    AACTGCGAGA TCAACATTAA TGAGTGCGAC AGTAATCCAT GCAGCAAGCA CGGAAATTGC
    AATGACGGGA TTGGAACCTA TACCTGCGAA TGCGAACCTG GCTTCGAGGG CACCCACTGT
    GAGATCAACA TAGATGAATG TGATCGCTAT AATCCCTGCC AGAGGGGCAC TTGCTATGAC
    CAGATAGACG ACTACGACTG CGACTGCGAT GCGAACTATG GAGGCAAAAA CTGTTCCGTT
    CTTCTCAAAG GCTGTGACCA GAATCCTTGT TTAAATGGCG GTGCCTGCTT GCCGTATTTG
    ATCAACGAGG TAACTCATCT GTACAACTGC ACCTGCGAGA ACGGCTTCCA GGGCGATAAA
    TGCGAGAAGA CCACCACTCT GTCCATGGTG GCCACTAGCC TGATTTCGGT TACCACGGAA
    CGCGAGGAAG GCTACGACAT TAATCTTCAA TTTAGGACCA CACTGCCCAA CGGAGTTTTG
    GCTTTCGGAA CCACCGGCGA AAAGAATGAA CCAGTTAGCT ATATTTTGGA GCTGATTAAT
    GGACGACTGA ATCTACATTC CTCGCTCCTA AATAAGTGGG AGGGCGTGTT TATTGGATCG
    AAGCTGAACG ATAGCAACTG GCACAAGGTG TTTGTGGCCA TAAACACTTC GCATCTAGTC
    CTTTCGGCCA ACGACGAGCA GGCTATTTTT CCGGTTGGCT CCTATGAAAC GGCCAATAAT
    AGCCAGCCTT CATTCCCGCG CACATACCTC GGAGGCACCA TTCCCAATCT GAAGTCCTAT
    CTGCGCCACC TCACCCACCA ACCATCAGCT TTTGTGGGCT GCATGCAAGA TATAATGGTC
    AATGGTAAAT GGATCTTTCC CGATGAGCAG GATGCGAATA TCAGTTATAC CAAACTGGAA
    AATGTCCAGA GCGGTTGTCC GCGCACCGAA CAATGTAAAC CGAATCCTTG CCATTCGAAC
    GGTGAATGCA CGGACCTTTG GCACACCTTC GCTTGTCACT GTCCCAGACC ATTCTTTGGG
    CACACATGTC AGCACAATAT GACTGCTGCC ACTTTTGGCC ACGAGAACAC AACCCATTCG
    GCCGTGATTG TGGAGACCAC AGATGTGGCT AGGCGAGCTA TTAGATCCAT CCTAGACATT
    TCCATGTTTA TTCGTACACG CGAGCCAACT GGTCAAGTCT TTTACTTGGG CACTGATCCA
    CGCAAAGCCC CCACTAAAAA TATTGGCGAC TCATATGTGG CCGCCAAATT ACACGGCGGC
    GAGCTGCTGG TAAAGATGCA GTTCAGCGGT ACTCCGGAGG CCTACACCGT TGGTGGCCAG
    AAATTGGACA ACGGCTATAA CCACCTGATC GAGGTGGTGC GCAATCAAAC GCTCGTGCAG
    GTCAAGCTCA ATGGCACCGA GTACTTCCGC AAGACGCTGT CCACGACGGG TCTGCTGGAC
    GCACAGGTGC TTTACTTGGG CGGACCTGCA CCCACACGCG AGTCACTTCT GGGCGCGACC
    ACAGAACCAG GAATAATTCC AGTTCCGGGA GCAGGAATAC CCATTGAAGA CACAACAGTG
    CCCAAGGAGG CGGACGACAG CAGGGACTAC TTTAAGGGCA TAATCCAGGA CGTGAAGGTT
    AGCAATGGCT CGCTCAACCT GATTGTGGAG ATGTATTCCC TAAACGTGAC AGATGTCCAA
    GTGAATGCCA AACCTCTGGG CGCTGTAACC ATCGATCGTG CCTCCGTGTT ACCTGGTGAG
    GTGTCCGATG ATCTGTGCCG AAAGAATCCC TGCCTCCATA ATGCCGAGTG CAGGAACACA
    TGGAATGACT ACACTTGCAA ATGCCCAAAT GGTTACAAGG GAAAGAACTG CCAGGAGATC
    GAATTTTGCC AACACGTTAC ATGTCCGGGA CAGAGCTTGT GCCAAAATCT GGACGATGGC
    TATGAGTGTG TTACGAACAC CACATTTACT GGGCAGGAAC GCAGTCCTCT CGCCTTCTTC
    TACTTCCAGG AGCAGCAATC TGACGATATT GTCAGTGAAG CGTCTCCCAA GCAAACTCTT
    AAACCGGTAA TTGATATTGC CTTCCGCACT CGTGCTGGAG GTACTCTCCT GTACATAGAC
    AATGTGGACG GATTCTTTGA AATTGGAGTA AACGGAGGAC GAGTGACCAT CACCTGGAAG
    CTAAGTGCAC TGCATTTTGG CGAGTCTGCA CGCTTTGAGA AGGAGAACAC TGACGGAGAA
    TGGAGTCGCA TTTACCTCAG GGCACACAAC AGCAAACTGG AGGGTGGATG GAAGGGATGG
    GAATCGATGG TGGATCCGAC GCCAGCTTTT TCCACGGACA TCGACCAAGC GGCCTTTCAG
    TCCCTGATCG CTACAAGTAC CCAGGTTTAC TTGGGTGGCA TGCCAGAGTC GCGACAAGCA
    CGAGGATCCA CTTTGTCTGC CCAGCAGGGC TCTCAGTTCA AGGGCTGTGT GGGAGAGGCA
    AGGGTGGGCG ATCTTTTACT GCCCTACTTC TCCATGGCGG AACTGTATTC GCGCACCAAT
    GTTTCAGTAC AGCAAAAGGC TCAATTCCGT CTAAATGCCA CACGACCTGA GGAGGGCTGC
    ATCCTGTGCT TCCAGTCAGA CTGCAAAAAT GACGGCTTCT GTCAATCTCC TTCAGATGAG
    TACGCCTGCA CCTGTCAGCC TGGATTCGAG GGCGATGATT GCGGCACGGA CATCGACGAG
    TGTCTTAACA CGGAATGCTT GAACAACGGC ACCTGCATCA ACCAGGTTGC AGCTTTCTTC
    TGCCAGTGTC AGCCAGGATT CGAGGGTCAG CACTGTGAGC AAAACATCGA CGAGTGTGCG
    GATCAGCCGT GCCACAACGG TGGCAACTGC ACGGATCTTA TCGCATCGTA CGTGTGCGAC
    TGCCCTGAGG ACTATATGGG CCCGCAGTGC GACGTGCTGA AGCAAATGAC CTGCGAGAAC
    GAGCCATGTC GGAATGGATC AACCTGCCAG AATGGATTCA ATGCTTCTAC TGGCAATAAC
    TTTACATGTA CATGCGTGCC CGGCTTCGAG GGTCCACTGT GTGACATACC CTTCTGTGAA
    ATAACGCCTT GCGATAACGG TGGCCTCTGC CTGACCACTG GAGCGGTACC GATGTGCAAA
    TGTAGTCTGG GATACACTGG TCGCCTGTGC GAGCAGGACA TTAACGAGTG CGAATCGAAT
    CCATGCCAGA ACGGTGGTCA GTGTAAGGAC CTCGTCGGCA GGTACGAGTG CGATTGCCAG
    GGCACCGGGT TCGAGGGCAT TCGCTGTGAA AATGACATCG ACGAGTGCAA CATGGAGGGA
    GATTACTGCG GCGGATTGGG CCGGTGTTTC AACAAGCCCG GATCCTTCCA GTGCATCTGC
    CAGAAACCCT ATTGCGGAGC CTACTGCAAC TTTACGGATC CCTGCAACGC TACGGACCTC
    TGCTCAAACG GCGGTCGCTG CGTAGAGTCC TGCGGCGCCA AACCGGACTA CTACTGCGAG
    TGTCCGGAAG GTTTCGCGGG AAAGAATTGC ACAGCACCGA TTACGGCCAA GGAGGACGGG
    CCTTCGACCA CAGACATTGC CATCATTGTA ATACCCGTAG TGGTGGTGCT GCTGCTGATC
    GCGGGAGCCC TCCTGGGCAC CTTCCTGGTG ATGGCCAGGA ACAAGCGAGC AACCAGGGGC
    ACCTATAGCC CGAGCGCGCA AGAGTACTGC AACCCACGGC TGGAAATGGA CAACGTACTG
    AAGCCACCGC CGGAAGAGCG ACTAATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001036751.1
    CDS550..7119
    Translation

    Target ORF information:

    RefSeq Version NM_001043286.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster crumbs (crb), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001043286.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGGCTAAAA TCGCCAATGC GTCACTGTCG CAACAACAAA AACAGCGGCA AGCAGAAACA 
    GCAACGACCA CAACAACAAC AGTGGCGGCA TCAGTAGAAA CAGCAACAAC AACAGCAAGA
    AGTCGAGACC GCACGAAAAG CGCAGCTCAA ATTACGTCGC ATTTGCTCAA GCGCGCCATT
    TCAGTTTATT CGTCGCCTCA ATGGATACCG CTCTTCATAC TTATTTACTT AGCAACAGAT
    GTCGCCTCAG TGGCGGTGCC GACGAAGGAG GCGTACTTTA ATGGCTCCAC TTACCTCCGC
    CTCACCACGC CGATGCCCAT TTGGGATCAC TCGGCGATTA GTTTCCGCTC GTGCCGCGGC
    GGCGAGATCC TCGCCCAGCA GTACAACAAG AACTCCATTG TAATCTCAGT GCTCAATGAC
    TTTCTGCAAA TCTCACTGGC TGGACCCGCC GTCCATGGGC CCAACAACCG GCTGGATGTC
    AAGCTGCCCT ACCAACTGCT GGACAACCGC TGGCATACGC TGCAGTTCAA GTACGAGTAC
    GGAAATCTCT ACCTGCATGT GGATCGCGCG GCAAGCATAT TTGCCAACTC CACGTACAAC
    AGTCAGTTCC TGACTAACCA GGACATTGGC TACAAGGACG CCATCTTGAT ACTGGGAAAC
    TCCTTCTCCG GTTGCCTTCT GGATGGACCA GGTCTGCAAT TTGTGAACAA CTCGACGGTG
    CAGAATGTGG TCTTTGGTCA CTGTCCCTTG ACCCCGGGTC CCTGCAGCGA TCACGATCTT
    TTCACTCGAC TGCCAGACAA CTTTTGTCTG AATGATCCCT GTATGGGCCA TGGAACCTGC
    TCGTCCAGTC CAGAGGGATA CGAATGTCGG TGCACGGCAC GTTACTCGGG AAAAAATTGC
    CAGAAGGACA ATGGCTCGCC GTGTGCCAAA AATCCATGTG AGAACGGTGG TTCCTGCCTG
    GAGAATTCCC GAGGGGATTA CCAGTGCTTC TGTGATCCCA ACCACAGTGG TCAGCACTGC
    GAGACGGAGG TGAACATCCA CCCACTCTGC CAGACGAACC CCTGCCTGAA CAATGGAGCA
    TGTGTGGTGA TTGGCGGCAG TGGAGCACTC ACCTGCGAGT GTCCCAAAGG ATACGCTGGT
    GCCAGGTGCG AGGTGGACAC GGATGAGTGC GCCTCACAGC CGTGTCAGAA CAACGGAAGC
    TGCATAGACC GTATCAATGG ATTCAGCTGC GATTGCAGCG GCACCGGTTA CACGGGTGCG
    TTCTGCCAGA CGAATGTGGA TGAGTGCGAC AAGAATCCGT GCCTGAACGG CGGCAGATGC
    TTCGATACCT ACGGATGGTA CACCTGCCAG TGTCTGGATG GTTGGGGCGG CGAGATTTGT
    GATCGACCCA TGACATGCCA GACGCAGCAG TGTTTGAACG GTGGCACCTG CCTGGACAAG
    CCCATCGGCT TCCAGTGCCT CTGTCCGCCG GAGTACACTG GTGAACTGTG CCAAATCGCA
    CCCAGTTGCG CACAGCAGTG CCCCATCGAT TCGGAGTGCG TAGGCGGCAA ATGCGTTTGT
    AAGCCAGGCT CATCGGCTTC CGACATGGAG CCGCTGACTC CGCTCGAGCT GGACATTCTC
    GACGCCACGC TGTGCCCCAG CGAAAAGAAG AAGCGCTACA TATCGCCCGA GTGGCTCAAA
    CGCAAGCGTT GTGAGCTAAA ACTAAGTTAC AACTGTCAAA CGAGCACGGG CGATGGAGCT
    TCTGCTTTGG CCCTGACACC CATCAACTGC AATGCCACCA ATGGAAAGTG TCTCAATGGA
    GGAACTTGCT CCATGAACGG AACCCACTGC TATTGCGCCG TCGGCTATTC GGGAGATCGT
    TGCGAGAAGG CCGAGAACTG TTCGCCACTG AACTGCCAGG AACCGATGGT ATGTGTCCAG
    AATCAGTGCC TCTGCCCGGA GAACAAGGTG TGCAACCAGT GCGCCACACA ACCGTGCCAG
    AATGGCGGTG AATGTGTGGA CCTACCGAAT GGAGACTACG AGTGCAAGTG CACCCGTGGA
    TGGACTGGAC GCACCTGTGG CAACGACGTG GACGAGTGCA CCCTGCATCC GAAGATCTGC
    GGCAATGGTA TCTGCAAGAA CGAGAAGGGA TCGTACAAAT GCTACTGTAC GCCTGGATTT
    ACCGGAGTCC ACTGCGATTC CGATGTGGAC GAATGCCTTA GCTTTCCCTG CCTCAACGGA
    GCCACGTGCC ACAACAAGAT AAATGCCTAC GAGTGCGTTT GCCAACCAGG ATATGAGGGA
    GAGAACTGCG AAGTAGATAT CGATGAGTGC GGCAGTAATC CCTGCTCGAA CGGATCCACC
    TGCATCGACA GGATCAACAA CTTCACCTGC AACTGCATTC CGGGAATGAC GGGCCGCATA
    TGTGACATCG ACATTGACGA CTGTGTGGGA GATCCCTGCT TGAATGGTGG TCAGTGCATC
    GACCAATTGG GTGGCTTCCG TTGTGACTGC AGTGGCACCG GTTATGAGGG CGAGAATTGT
    GAGCTGAACA TAGACGAATG CCTCTCGAAT CCGTGCACGA ATGGTGCCAA GTGCCTGGAC
    AGGGTTAAGG ACTACTTCTG CGATTGCCAC AACGGCTACA AGGGAAAGAA CTGTGAGCAG
    GACATCAACG AGTGCGAGAG TAATCCCTGC CAGTACAATG GCAACTGCCT AGAGCGTTCG
    AACATAACGC TTTACCAAAT GAGTAGGATC ACGGATCTGC CCAAGGTGTT TAGCCAGCCC
    TTTAGCTTTG AGAATGCCAG CGGCTACGAG TGCGTCTGTG TGCCCGGCAT CATTGGAAAG
    AACTGCGAGA TCAACATTAA TGAGTGCGAC AGTAATCCAT GCAGCAAGCA CGGAAATTGC
    AATGACGGGA TTGGAACCTA TACCTGCGAA TGCGAACCTG GCTTCGAGGG CACCCACTGT
    GAGATCAACA TAGATGAATG TGATCGCTAT AATCCCTGCC AGAGGGGCAC TTGCTATGAC
    CAGATAGACG ACTACGACTG CGACTGCGAT GCGAACTATG GAGGCAAAAA CTGTTCCGTT
    CTTCTCAAAG GCTGTGACCA GAATCCTTGT TTAAATGGCG GTGCCTGCTT GCCGTATTTG
    ATCAACGAGG TAACTCATCT GTACAACTGC ACCTGCGAGA ACGGCTTCCA GGGCGATAAA
    TGCGAGAAGA CCACCACTCT GTCCATGGTG GCCACTAGCC TGATTTCGGT TACCACGGAA
    CGCGAGGAAG GCTACGACAT TAATCTTCAA TTTAGGACCA CACTGCCCAA CGGAGTTTTG
    GCTTTCGGAA CCACCGGCGA AAAGAATGAA CCAGTTAGCT ATATTTTGGA GCTGATTAAT
    GGACGACTGA ATCTACATTC CTCGCTCCTA AATAAGTGGG AGGGCGTGTT TATTGGATCG
    AAGCTGAACG ATAGCAACTG GCACAAGGTG TTTGTGGCCA TAAACACTTC GCATCTAGTC
    CTTTCGGCCA ACGACGAGCA GGCTATTTTT CCGGTTGGCT CCTATGAAAC GGCCAATAAT
    AGCCAGCCTT CATTCCCGCG CACATACCTC GGAGGCACCA TTCCCAATCT GAAGTCCTAT
    CTGCGCCACC TCACCCACCA ACCATCAGCT TTTGTGGGCT GCATGCAAGA TATAATGGTC
    AATGGTAAAT GGATCTTTCC CGATGAGCAG GATGCGAATA TCAGTTATAC CAAACTGGAA
    AATGTCCAGA GCGGTTGTCC GCGCACCGAA CAATGTAAAC CGAATCCTTG CCATTCGAAC
    GGTGAATGCA CGGACCTTTG GCACACCTTC GCTTGTCACT GTCCCAGACC ATTCTTTGGG
    CACACATGTC AGCACAATAT GACTGCTGCC ACTTTTGGCC ACGAGAACAC AACCCATTCG
    GCCGTGATTG TGGAGACCAC AGATGTGGCT AGGCGAGCTA TTAGATCCAT CCTAGACATT
    TCCATGTTTA TTCGTACACG CGAGCCAACT GGTCAAGTCT TTTACTTGGG CACTGATCCA
    CGCAAAGCCC CCACTAAAAA TATTGGCGAC TCATATGTGG CCGCCAAATT ACACGGCGGC
    GAGCTGCTGG TAAAGATGCA GTTCAGCGGT ACTCCGGAGG CCTACACCGT TGGTGGCCAG
    AAATTGGACA ACGGCTATAA CCACCTGATC GAGGTGGTGC GCAATCAAAC GCTCGTGCAG
    GTCAAGCTCA ATGGCACCGA GTACTTCCGC AAGACGCTGT CCACGACGGG TCTGCTGGAC
    GCACAGGTGC TTTACTTGGG CGGACCTGCA CCCACACGCG AGTCACTTCT GGGCGCGACC
    ACAGAACCAG GAATAATTCC AGTTCCGGGA GCAGGAATAC CCATTGAAGA CACAACAGTG
    CCCAAGGAGG CGGACGACAG CAGGGACTAC TTTAAGGGCA TAATCCAGGA CGTGAAGGTT
    AGCAATGGCT CGCTCAACCT GATTGTGGAG ATGTATTCCC TAAACGTGAC AGATGTCCAA
    GTGAATGCCA AACCTCTGGG CGCTGTAACC ATCGATCGTG CCTCCGTGTT ACCTGGTGAG
    GTGTCCGATG ATCTGTGCCG AAAGAATCCC TGCCTCCATA ATGCCGAGTG CAGGAACACA
    TGGAATGACT ACACTTGCAA ATGCCCAAAT GGTTACAAGG GAAAGAACTG CCAGGAGATC
    GAATTTTGCC AACACGTTAC ATGTCCGGGA CAGAGCTTGT GCCAAAATCT GGACGATGGC
    TATGAGTGTG TTACGAACAC CACATTTACT GGGCAGGAAC GCAGTCCTCT CGCCTTCTTC
    TACTTCCAGG AGCAGCAATC TGACGATATT GTCAGTGAAG CGTCTCCCAA GCAAACTCTT
    AAACCGGTAA TTGATATTGC CTTCCGCACT CGTGCTGGAG GTACTCTCCT GTACATAGAC
    AATGTGGACG GATTCTTTGA AATTGGAGTA AACGGAGGAC GAGTGACCAT CACCTGGAAG
    CTAAGTGCAC TGCATTTTGG CGAGTCTGCA CGCTTTGAGA AGGAGAACAC TGACGGAGAA
    TGGAGTCGCA TTTACCTCAG GGCACACAAC AGCAAACTGG AGGGTGGATG GAAGGGATGG
    GAATCGATGG TGGATCCGAC GCCAGCTTTT TCCACGGACA TCGACCAAGC GGCCTTTCAG
    TCCCTGATCG CTACAAGTAC CCAGGTTTAC TTGGGTGGCA TGCCAGAGTC GCGACAAGCA
    CGAGGATCCA CTTTGTCTGC CCAGCAGGGC TCTCAGTTCA AGGGCTGTGT GGGAGAGGCA
    AGGGTGGGCG ATCTTTTACT GCCCTACTTC TCCATGGCGG AACTGTATTC GCGCACCAAT
    GTTTCAGTAC AGCAAAAGGC TCAATTCCGT CTAAATGCCA CACGACCTGA GGAGGGCTGC
    ATCCTGTGCT TCCAGTCAGA CTGCAAAAAT GACGGCTTCT GTCAATCTCC TTCAGATGAG
    TACGCCTGCA CCTGTCAGCC TGGATTCGAG GGCGATGATT GCGGCACGGA CATCGACGAG
    TGTCTTAACA CGGAATGCTT GAACAACGGC ACCTGCATCA ACCAGGTTGC AGCTTTCTTC
    TGCCAGTGTC AGCCAGGATT CGAGGGTCAG CACTGTGAGC AAAACATCGA CGAGTGTGCG
    GATCAGCCGT GCCACAACGG TGGCAACTGC ACGGATCTTA TCGCATCGTA CGTGTGCGAC
    TGCCCTGAGG ACTATATGGG CCCGCAGTGC GACGTGCTGA AGCAAATGAC CTGCGAGAAC
    GAGCCATGTC GGAATGGATC AACCTGCCAG AATGGATTCA ATGCTTCTAC TGGCAATAAC
    TTTACATGTA CATGCGTGCC CGGCTTCGAG GGTCCACTGT GTGACATACC CTTCTGTGAA
    ATAACGCCTT GCGATAACGG TGGCCTCTGC CTGACCACTG GAGCGGTACC GATGTGCAAA
    TGTAGTCTGG GATACACTGG TCGCCTGTGC GAGCAGGACA TTAACGAGTG CGAATCGAAT
    CCATGCCAGA ACGGTGGTCA GTGTAAGGAC CTCGTCGGCA GGTACGAGTG CGATTGCCAG
    GGCACCGGGT TCGAGGGCAT TCGCTGTGAA AATGACATCG ACGAGTGCAA CATGGAGGGA
    GATTACTGCG GCGGATTGGG CCGGTGTTTC AACAAGCCCG GATCCTTCCA GTGCATCTGC
    CAGAAACCCT ATTGCGGAGC CTACTGCAAC TTTACGGATC CCTGCAACGC TACGGACCTC
    TGCTCAAACG GCGGTCGCTG CGTAGAGTCC TGCGGCGCCA AACCGGACTA CTACTGCGAG
    TGTCCGGAAG GTTTCGCGGG AAAGAATTGC ACAGCACCGA TTACGGCCAA GGAGGACGGG
    CCTTCGACCA CAGACATTGC CATCATTGTA ATACCCGTAG TGGTGGTGCT GCTGCTGATC
    GCGGGAGCCC TCCTGGGCAC CTTCCTGGTG ATGGCCAGGA ACAAGCGAGC AACCAGGGGC
    ACCTATAGCC CGAGCGCGCA AGAGTACTGC AACCCACGGC TGGAAATGGA CAACGTACTG
    AAGCCACCGC CGGAAGAGCG ACTAATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa05935
    Clone ID Related Accession (Same CDS sequence) NM_079756.3
    Accession Version NM_079756.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6441bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product crumbs, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_079756.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCTAAAA TCGCCAATGC GTCACTGTCG CAACAACAAA AACAGCGGCA AGCAGAAACA 
    GCAACGACCA CAACAACAAC AGTGGCGGCA TCAGTAGAAA CAGCAACAAC AACAGCAAGA
    AGTCGAGACC GCACGAAAAG CGCAGCTCAA ATTACGTCGC ATTTGCTCAA GCGCGCCATT
    TCAGTTTATT CGTCGCCTCA ATGGATACCG CTCTTCATAC TTATTTACTT AGCAACAGAT
    GTCGCCTCAG TGGCGGTGCC GACGAAGGAG GCGTACTTTA ATGGCTCCAC TTACCTCCGC
    CTCACCACGC CGATGCCCAT TTGGGATCAC TCGGCGATTA GTTTCCGCTC GTGCCGCGGC
    GGCGAGATCC TCGCCCAGCA GTACAACAAG AACTCCATTG TAATCTCAGT GCTCAATGAC
    TTTCTGCAAA TCTCACTGGC TGGACCCGCC GTCCATGGGC CCAACAACCG GCTGGATGTC
    AAGCTGCCCT ACCAACTGCT GGACAACCGC TGGCATACGC TGCAGTTCAA GTACGAGTAC
    GGAAATCTCT ACCTGCATGT GGATCGCGCG GCAAGCATAT TTGCCAACTC CACGTACAAC
    AGTCAGTTCC TGACTAACCA GGACATTGGC TACAAGGACG CCATCTTGAT ACTGGGAAAC
    TCCTTCTCCG GTTGCCTTCT GGATGGACCA GGTCTGCAAT TTGTGAACAA CTCGACGGTG
    CAGAATGTGG TCTTTGGTCA CTGTCCCTTG ACCCCGGGTC CCTGCAGCGA TCACGATCTT
    TTCACTCGAC TGCCAGACAA CTTTTGTCTG AATGATCCCT GTATGGGCCA TGGAACCTGC
    TCGTCCAGTC CAGAGGGATA CGAATGTCGG TGCACGGCAC GTTACTCGGG AAAAAATTGC
    CAGAAGGACA ATGGCTCGCC GTGTGCCAAA AATCCATGTG AGAACGGTGG TTCCTGCCTG
    GAGAATTCCC GAGGGGATTA CCAGTGCTTC TGTGATCCCA ACCACAGTGG TCAGCACTGC
    GAGACGGAGG TGAACATCCA CCCACTCTGC CAGACGAACC CCTGCCTGAA CAATGGAGCA
    TGTGTGGTGA TTGGCGGCAG TGGAGCACTC ACCTGCGAGT GTCCCAAAGG ATACGCTGGT
    GCCAGGTGCG AGGTGGACAC GGATGAGTGC GCCTCACAGC CGTGTCAGAA CAACGGAAGC
    TGCATAGACC GTATCAATGG ATTCAGCTGC GATTGCAGCG GCACCGGTTA CACGGGTGCG
    TTCTGCCAGA CGAATGTGGA TGAGTGCGAC AAGAATCCGT GCCTGAACGG CGGCAGATGC
    TTCGATACCT ACGGATGGTA CACCTGCCAG TGTCTGGATG GTTGGGGCGG CGAGATTTGT
    GATCGACCCA TGACATGCCA GACGCAGCAG TGTTTGAACG GTGGCACCTG CCTGGACAAG
    CCCATCGGCT TCCAGTGCCT CTGTCCGCCG GAGTACACTG GTGAACTGTG CCAAATCGCA
    CCCAGTTGCG CACAGCAGTG CCCCATCGAT TCGGAGTGCG TAGGCGGCAA ATGCGTTTGT
    AAGCCAGGCT CATCGGGTTA CAACTGTCAA ACGAGCACGG GCGATGGAGC TTCTGCTTTG
    GCCCTGACAC CCATCAACTG CAATGCCACC AATGGAAAGT GTCTCAATGG AGGAACTTGC
    TCCATGAACG GAACCCACTG CTATTGCGCC GTCGGCTATT CGGGAGATCG TTGCGAGAAG
    GCCGAGAACT GTTCGCCACT GAACTGCCAG GAACCGATGG TATGTGTCCA GAATCAGTGC
    CTCTGCCCGG AGAACAAGGT GTGCAACCAG TGCGCCACAC AACCGTGCCA GAATGGCGGT
    GAATGTGTGG ACCTACCGAA TGGAGACTAC GAGTGCAAGT GCACCCGTGG ATGGACTGGA
    CGCACCTGTG GCAACGACGT GGACGAGTGC ACCCTGCATC CGAAGATCTG CGGCAATGGT
    ATCTGCAAGA ACGAGAAGGG ATCGTACAAA TGCTACTGTA CGCCTGGATT TACCGGAGTC
    CACTGCGATT CCGATGTGGA CGAATGCCTT AGCTTTCCCT GCCTCAACGG AGCCACGTGC
    CACAACAAGA TAAATGCCTA CGAGTGCGTT TGCCAACCAG GATATGAGGG AGAGAACTGC
    GAAGTAGATA TCGATGAGTG CGGCAGTAAT CCCTGCTCGA ACGGATCCAC CTGCATCGAC
    AGGATCAACA ACTTCACCTG CAACTGCATT CCGGGAATGA CGGGCCGCAT ATGTGACATC
    GACATTGACG ACTGTGTGGG AGATCCCTGC TTGAATGGTG GTCAGTGCAT CGACCAATTG
    GGTGGCTTCC GTTGTGACTG CAGTGGCACC GGTTATGAGG GCGAGAATTG TGAGCTGAAC
    ATAGACGAAT GCCTCTCGAA TCCGTGCACG AATGGTGCCA AGTGCCTGGA CAGGGTTAAG
    GACTACTTCT GCGATTGCCA CAACGGCTAC AAGGGAAAGA ACTGTGAGCA GGACATCAAC
    GAGTGCGAGA GTAATCCCTG CCAGTACAAT GGCAACTGCC TAGAGCGTTC GAACATAACG
    CTTTACCAAA TGAGTAGGAT CACGGATCTG CCCAAGGTGT TTAGCCAGCC CTTTAGCTTT
    GAGAATGCCA GCGGCTACGA GTGCGTCTGT GTGCCCGGCA TCATTGGAAA GAACTGCGAG
    ATCAACATTA ATGAGTGCGA CAGTAATCCA TGCAGCAAGC ACGGAAATTG CAATGACGGG
    ATTGGAACCT ATACCTGCGA ATGCGAACCT GGCTTCGAGG GCACCCACTG TGAGATCAAC
    ATAGATGAAT GTGATCGCTA TAATCCCTGC CAGAGGGGCA CTTGCTATGA CCAGATAGAC
    GACTACGACT GCGACTGCGA TGCGAACTAT GGAGGCAAAA ACTGTTCCGT TCTTCTCAAA
    GGCTGTGACC AGAATCCTTG TTTAAATGGC GGTGCCTGCT TGCCGTATTT GATCAACGAG
    GTAACTCATC TGTACAACTG CACCTGCGAG AACGGCTTCC AGGGCGATAA ATGCGAGAAG
    ACCACCACTC TGTCCATGGT GGCCACTAGC CTGATTTCGG TTACCACGGA ACGCGAGGAA
    GGCTACGACA TTAATCTTCA ATTTAGGACC ACACTGCCCA ACGGAGTTTT GGCTTTCGGA
    ACCACCGGCG AAAAGAATGA ACCAGTTAGC TATATTTTGG AGCTGATTAA TGGACGACTG
    AATCTACATT CCTCGCTCCT AAATAAGTGG GAGGGCGTGT TTATTGGATC GAAGCTGAAC
    GATAGCAACT GGCACAAGGT GTTTGTGGCC ATAAACACTT CGCATCTAGT CCTTTCGGCC
    AACGACGAGC AGGCTATTTT TCCGGTTGGC TCCTATGAAA CGGCCAATAA TAGCCAGCCT
    TCATTCCCGC GCACATACCT CGGAGGCACC ATTCCCAATC TGAAGTCCTA TCTGCGCCAC
    CTCACCCACC AACCATCAGC TTTTGTGGGC TGCATGCAAG ATATAATGGT CAATGGTAAA
    TGGATCTTTC CCGATGAGCA GGATGCGAAT ATCAGTTATA CCAAACTGGA AAATGTCCAG
    AGCGGTTGTC CGCGCACCGA ACAATGTAAA CCGAATCCTT GCCATTCGAA CGGTGAATGC
    ACGGACCTTT GGCACACCTT CGCTTGTCAC TGTCCCAGAC CATTCTTTGG GCACACATGT
    CAGCACAATA TGACTGCTGC CACTTTTGGC CACGAGAACA CAACCCATTC GGCCGTGATT
    GTGGAGACCA CAGATGTGGC TAGGCGAGCT ATTAGATCCA TCCTAGACAT TTCCATGTTT
    ATTCGTACAC GCGAGCCAAC TGGTCAAGTC TTTTACTTGG GCACTGATCC ACGCAAAGCC
    CCCACTAAAA ATATTGGCGA CTCATATGTG GCCGCCAAAT TACACGGCGG CGAGCTGCTG
    GTAAAGATGC AGTTCAGCGG TACTCCGGAG GCCTACACCG TTGGTGGCCA GAAATTGGAC
    AACGGCTATA ACCACCTGAT CGAGGTGGTG CGCAATCAAA CGCTCGTGCA GGTCAAGCTC
    AATGGCACCG AGTACTTCCG CAAGACGCTG TCCACGACGG GTCTGCTGGA CGCACAGGTG
    CTTTACTTGG GCGGACCTGC ACCCACACGC GAGTCACTTC TGGGCGCGAC CACAGAACCA
    GGAATAATTC CAGTTCCGGG AGCAGGAATA CCCATTGAAG ACACAACAGT GCCCAAGGAG
    GCGGACGACA GCAGGGACTA CTTTAAGGGC ATAATCCAGG ACGTGAAGGT TAGCAATGGC
    TCGCTCAACC TGATTGTGGA GATGTATTCC CTAAACGTGA CAGATGTCCA AGTGAATGCC
    AAACCTCTGG GCGCTGTAAC CATCGATCGT GCCTCCGTGT TACCTGGTGA GGTGTCCGAT
    GATCTGTGCC GAAAGAATCC CTGCCTCCAT AATGCCGAGT GCAGGAACAC ATGGAATGAC
    TACACTTGCA AATGCCCAAA TGGTTACAAG GGAAAGAACT GCCAGGAGAT CGAATTTTGC
    CAACACGTTA CATGTCCGGG ACAGAGCTTG TGCCAAAATC TGGACGATGG CTATGAGTGT
    GTTACGAACA CCACATTTAC TGGGCAGGAA CGCAGTCCTC TCGCCTTCTT CTACTTCCAG
    GAGCAGCAAT CTGACGATAT TGTCAGTGAA GCGTCTCCCA AGCAAACTCT TAAACCGGTA
    ATTGATATTG CCTTCCGCAC TCGTGCTGGA GGTACTCTCC TGTACATAGA CAATGTGGAC
    GGATTCTTTG AAATTGGAGT AAACGGAGGA CGAGTGACCA TCACCTGGAA GCTAAGTGCA
    CTGCATTTTG GCGAGTCTGC ACGCTTTGAG AAGGAGAACA CTGACGGAGA ATGGAGTCGC
    ATTTACCTCA GGGCACACAA CAGCAAACTG GAGGGTGGAT GGAAGGGATG GGAATCGATG
    GTGGATCCGA CGCCAGCTTT TTCCACGGAC ATCGACCAAG CGGCCTTTCA GTCCCTGATC
    GCTACAAGTA CCCAGGTTTA CTTGGGTGGC ATGCCAGAGT CGCGACAAGC ACGAGGATCC
    ACTTTGTCTG CCCAGCAGGG CTCTCAGTTC AAGGGCTGTG TGGGAGAGGC AAGGGTGGGC
    GATCTTTTAC TGCCCTACTT CTCCATGGCG GAACTGTATT CGCGCACCAA TGTTTCAGTA
    CAGCAAAAGG CTCAATTCCG TCTAAATGCC ACACGACCTG AGGAGGGCTG CATCCTGTGC
    TTCCAGTCAG ACTGCAAAAA TGACGGCTTC TGTCAATCTC CTTCAGATGA GTACGCCTGC
    ACCTGTCAGC CTGGATTCGA GGGCGATGAT TGCGGCACGG ACATCGACGA GTGTCTTAAC
    ACGGAATGCT TGAACAACGG CACCTGCATC AACCAGGTTG CAGCTTTCTT CTGCCAGTGT
    CAGCCAGGAT TCGAGGGTCA GCACTGTGAG CAAAACATCG ACGAGTGTGC GGATCAGCCG
    TGCCACAACG GTGGCAACTG CACGGATCTT ATCGCATCGT ACGTGTGCGA CTGCCCTGAG
    GACTATATGG GCCCGCAGTG CGACGTGCTG AAGCAAATGA CCTGCGAGAA CGAGCCATGT
    CGGAATGGAT CAACCTGCCA GAATGGATTC AATGCTTCTA CTGGCAATAA CTTTACATGT
    ACATGCGTGC CCGGCTTCGA GGGTCCACTG TGTGACATAC CCTTCTGTGA AATAACGCCT
    TGCGATAACG GTGGCCTCTG CCTGACCACT GGAGCGGTAC CGATGTGCAA ATGTAGTCTG
    GGATACACTG GTCGCCTGTG CGAGCAGGAC ATTAACGAGT GCGAATCGAA TCCATGCCAG
    AACGGTGGTC AGTGTAAGGA CCTCGTCGGC AGGTACGAGT GCGATTGCCA GGGCACCGGG
    TTCGAGGGCA TTCGCTGTGA AAATGACATC GACGAGTGCA ACATGGAGGG AGATTACTGC
    GGCGGATTGG GCCGGTGTTT CAACAAGCCC GGATCCTTCC AGTGCATCTG CCAGAAACCC
    TATTGCGGAG CCTACTGCAA CTTTACGGAT CCCTGCAACG CTACGGACCT CTGCTCAAAC
    GGCGGTCGCT GCGTAGAGTC CTGCGGCGCC AAACCGGACT ACTACTGCGA GTGTCCGGAA
    GGTTTCGCGG GAAAGAATTG CACAGCACCG ATTACGGCCA AGGAGGACGG GCCTTCGACC
    ACAGACATTG CCATCATTGT AATACCCGTA GTGGTGGTGC TGCTGCTGAT CGCGGGAGCC
    CTCCTGGGCA CCTTCCTGGT GATGGCCAGG AACAAGCGAG CAACCAGGGG CACCTATAGC
    CCGAGCGCGC AAGAGTACTG CAACCCACGG CTGGAAATGG ACAACGTACT GAAGCCACCG
    CCGGAAGAGC GACTAATTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_524480.2
    CDS550..6990
    Translation

    Target ORF information:

    RefSeq Version NM_079756.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster crumbs (crb), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_079756.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCTAAAA TCGCCAATGC GTCACTGTCG CAACAACAAA AACAGCGGCA AGCAGAAACA 
    GCAACGACCA CAACAACAAC AGTGGCGGCA TCAGTAGAAA CAGCAACAAC AACAGCAAGA
    AGTCGAGACC GCACGAAAAG CGCAGCTCAA ATTACGTCGC ATTTGCTCAA GCGCGCCATT
    TCAGTTTATT CGTCGCCTCA ATGGATACCG CTCTTCATAC TTATTTACTT AGCAACAGAT
    GTCGCCTCAG TGGCGGTGCC GACGAAGGAG GCGTACTTTA ATGGCTCCAC TTACCTCCGC
    CTCACCACGC CGATGCCCAT TTGGGATCAC TCGGCGATTA GTTTCCGCTC GTGCCGCGGC
    GGCGAGATCC TCGCCCAGCA GTACAACAAG AACTCCATTG TAATCTCAGT GCTCAATGAC
    TTTCTGCAAA TCTCACTGGC TGGACCCGCC GTCCATGGGC CCAACAACCG GCTGGATGTC
    AAGCTGCCCT ACCAACTGCT GGACAACCGC TGGCATACGC TGCAGTTCAA GTACGAGTAC
    GGAAATCTCT ACCTGCATGT GGATCGCGCG GCAAGCATAT TTGCCAACTC CACGTACAAC
    AGTCAGTTCC TGACTAACCA GGACATTGGC TACAAGGACG CCATCTTGAT ACTGGGAAAC
    TCCTTCTCCG GTTGCCTTCT GGATGGACCA GGTCTGCAAT TTGTGAACAA CTCGACGGTG
    CAGAATGTGG TCTTTGGTCA CTGTCCCTTG ACCCCGGGTC CCTGCAGCGA TCACGATCTT
    TTCACTCGAC TGCCAGACAA CTTTTGTCTG AATGATCCCT GTATGGGCCA TGGAACCTGC
    TCGTCCAGTC CAGAGGGATA CGAATGTCGG TGCACGGCAC GTTACTCGGG AAAAAATTGC
    CAGAAGGACA ATGGCTCGCC GTGTGCCAAA AATCCATGTG AGAACGGTGG TTCCTGCCTG
    GAGAATTCCC GAGGGGATTA CCAGTGCTTC TGTGATCCCA ACCACAGTGG TCAGCACTGC
    GAGACGGAGG TGAACATCCA CCCACTCTGC CAGACGAACC CCTGCCTGAA CAATGGAGCA
    TGTGTGGTGA TTGGCGGCAG TGGAGCACTC ACCTGCGAGT GTCCCAAAGG ATACGCTGGT
    GCCAGGTGCG AGGTGGACAC GGATGAGTGC GCCTCACAGC CGTGTCAGAA CAACGGAAGC
    TGCATAGACC GTATCAATGG ATTCAGCTGC GATTGCAGCG GCACCGGTTA CACGGGTGCG
    TTCTGCCAGA CGAATGTGGA TGAGTGCGAC AAGAATCCGT GCCTGAACGG CGGCAGATGC
    TTCGATACCT ACGGATGGTA CACCTGCCAG TGTCTGGATG GTTGGGGCGG CGAGATTTGT
    GATCGACCCA TGACATGCCA GACGCAGCAG TGTTTGAACG GTGGCACCTG CCTGGACAAG
    CCCATCGGCT TCCAGTGCCT CTGTCCGCCG GAGTACACTG GTGAACTGTG CCAAATCGCA
    CCCAGTTGCG CACAGCAGTG CCCCATCGAT TCGGAGTGCG TAGGCGGCAA ATGCGTTTGT
    AAGCCAGGCT CATCGGGTTA CAACTGTCAA ACGAGCACGG GCGATGGAGC TTCTGCTTTG
    GCCCTGACAC CCATCAACTG CAATGCCACC AATGGAAAGT GTCTCAATGG AGGAACTTGC
    TCCATGAACG GAACCCACTG CTATTGCGCC GTCGGCTATT CGGGAGATCG TTGCGAGAAG
    GCCGAGAACT GTTCGCCACT GAACTGCCAG GAACCGATGG TATGTGTCCA GAATCAGTGC
    CTCTGCCCGG AGAACAAGGT GTGCAACCAG TGCGCCACAC AACCGTGCCA GAATGGCGGT
    GAATGTGTGG ACCTACCGAA TGGAGACTAC GAGTGCAAGT GCACCCGTGG ATGGACTGGA
    CGCACCTGTG GCAACGACGT GGACGAGTGC ACCCTGCATC CGAAGATCTG CGGCAATGGT
    ATCTGCAAGA ACGAGAAGGG ATCGTACAAA TGCTACTGTA CGCCTGGATT TACCGGAGTC
    CACTGCGATT CCGATGTGGA CGAATGCCTT AGCTTTCCCT GCCTCAACGG AGCCACGTGC
    CACAACAAGA TAAATGCCTA CGAGTGCGTT TGCCAACCAG GATATGAGGG AGAGAACTGC
    GAAGTAGATA TCGATGAGTG CGGCAGTAAT CCCTGCTCGA ACGGATCCAC CTGCATCGAC
    AGGATCAACA ACTTCACCTG CAACTGCATT CCGGGAATGA CGGGCCGCAT ATGTGACATC
    GACATTGACG ACTGTGTGGG AGATCCCTGC TTGAATGGTG GTCAGTGCAT CGACCAATTG
    GGTGGCTTCC GTTGTGACTG CAGTGGCACC GGTTATGAGG GCGAGAATTG TGAGCTGAAC
    ATAGACGAAT GCCTCTCGAA TCCGTGCACG AATGGTGCCA AGTGCCTGGA CAGGGTTAAG
    GACTACTTCT GCGATTGCCA CAACGGCTAC AAGGGAAAGA ACTGTGAGCA GGACATCAAC
    GAGTGCGAGA GTAATCCCTG CCAGTACAAT GGCAACTGCC TAGAGCGTTC GAACATAACG
    CTTTACCAAA TGAGTAGGAT CACGGATCTG CCCAAGGTGT TTAGCCAGCC CTTTAGCTTT
    GAGAATGCCA GCGGCTACGA GTGCGTCTGT GTGCCCGGCA TCATTGGAAA GAACTGCGAG
    ATCAACATTA ATGAGTGCGA CAGTAATCCA TGCAGCAAGC ACGGAAATTG CAATGACGGG
    ATTGGAACCT ATACCTGCGA ATGCGAACCT GGCTTCGAGG GCACCCACTG TGAGATCAAC
    ATAGATGAAT GTGATCGCTA TAATCCCTGC CAGAGGGGCA CTTGCTATGA CCAGATAGAC
    GACTACGACT GCGACTGCGA TGCGAACTAT GGAGGCAAAA ACTGTTCCGT TCTTCTCAAA
    GGCTGTGACC AGAATCCTTG TTTAAATGGC GGTGCCTGCT TGCCGTATTT GATCAACGAG
    GTAACTCATC TGTACAACTG CACCTGCGAG AACGGCTTCC AGGGCGATAA ATGCGAGAAG
    ACCACCACTC TGTCCATGGT GGCCACTAGC CTGATTTCGG TTACCACGGA ACGCGAGGAA
    GGCTACGACA TTAATCTTCA ATTTAGGACC ACACTGCCCA ACGGAGTTTT GGCTTTCGGA
    ACCACCGGCG AAAAGAATGA ACCAGTTAGC TATATTTTGG AGCTGATTAA TGGACGACTG
    AATCTACATT CCTCGCTCCT AAATAAGTGG GAGGGCGTGT TTATTGGATC GAAGCTGAAC
    GATAGCAACT GGCACAAGGT GTTTGTGGCC ATAAACACTT CGCATCTAGT CCTTTCGGCC
    AACGACGAGC AGGCTATTTT TCCGGTTGGC TCCTATGAAA CGGCCAATAA TAGCCAGCCT
    TCATTCCCGC GCACATACCT CGGAGGCACC ATTCCCAATC TGAAGTCCTA TCTGCGCCAC
    CTCACCCACC AACCATCAGC TTTTGTGGGC TGCATGCAAG ATATAATGGT CAATGGTAAA
    TGGATCTTTC CCGATGAGCA GGATGCGAAT ATCAGTTATA CCAAACTGGA AAATGTCCAG
    AGCGGTTGTC CGCGCACCGA ACAATGTAAA CCGAATCCTT GCCATTCGAA CGGTGAATGC
    ACGGACCTTT GGCACACCTT CGCTTGTCAC TGTCCCAGAC CATTCTTTGG GCACACATGT
    CAGCACAATA TGACTGCTGC CACTTTTGGC CACGAGAACA CAACCCATTC GGCCGTGATT
    GTGGAGACCA CAGATGTGGC TAGGCGAGCT ATTAGATCCA TCCTAGACAT TTCCATGTTT
    ATTCGTACAC GCGAGCCAAC TGGTCAAGTC TTTTACTTGG GCACTGATCC ACGCAAAGCC
    CCCACTAAAA ATATTGGCGA CTCATATGTG GCCGCCAAAT TACACGGCGG CGAGCTGCTG
    GTAAAGATGC AGTTCAGCGG TACTCCGGAG GCCTACACCG TTGGTGGCCA GAAATTGGAC
    AACGGCTATA ACCACCTGAT CGAGGTGGTG CGCAATCAAA CGCTCGTGCA GGTCAAGCTC
    AATGGCACCG AGTACTTCCG CAAGACGCTG TCCACGACGG GTCTGCTGGA CGCACAGGTG
    CTTTACTTGG GCGGACCTGC ACCCACACGC GAGTCACTTC TGGGCGCGAC CACAGAACCA
    GGAATAATTC CAGTTCCGGG AGCAGGAATA CCCATTGAAG ACACAACAGT GCCCAAGGAG
    GCGGACGACA GCAGGGACTA CTTTAAGGGC ATAATCCAGG ACGTGAAGGT TAGCAATGGC
    TCGCTCAACC TGATTGTGGA GATGTATTCC CTAAACGTGA CAGATGTCCA AGTGAATGCC
    AAACCTCTGG GCGCTGTAAC CATCGATCGT GCCTCCGTGT TACCTGGTGA GGTGTCCGAT
    GATCTGTGCC GAAAGAATCC CTGCCTCCAT AATGCCGAGT GCAGGAACAC ATGGAATGAC
    TACACTTGCA AATGCCCAAA TGGTTACAAG GGAAAGAACT GCCAGGAGAT CGAATTTTGC
    CAACACGTTA CATGTCCGGG ACAGAGCTTG TGCCAAAATC TGGACGATGG CTATGAGTGT
    GTTACGAACA CCACATTTAC TGGGCAGGAA CGCAGTCCTC TCGCCTTCTT CTACTTCCAG
    GAGCAGCAAT CTGACGATAT TGTCAGTGAA GCGTCTCCCA AGCAAACTCT TAAACCGGTA
    ATTGATATTG CCTTCCGCAC TCGTGCTGGA GGTACTCTCC TGTACATAGA CAATGTGGAC
    GGATTCTTTG AAATTGGAGT AAACGGAGGA CGAGTGACCA TCACCTGGAA GCTAAGTGCA
    CTGCATTTTG GCGAGTCTGC ACGCTTTGAG AAGGAGAACA CTGACGGAGA ATGGAGTCGC
    ATTTACCTCA GGGCACACAA CAGCAAACTG GAGGGTGGAT GGAAGGGATG GGAATCGATG
    GTGGATCCGA CGCCAGCTTT TTCCACGGAC ATCGACCAAG CGGCCTTTCA GTCCCTGATC
    GCTACAAGTA CCCAGGTTTA CTTGGGTGGC ATGCCAGAGT CGCGACAAGC ACGAGGATCC
    ACTTTGTCTG CCCAGCAGGG CTCTCAGTTC AAGGGCTGTG TGGGAGAGGC AAGGGTGGGC
    GATCTTTTAC TGCCCTACTT CTCCATGGCG GAACTGTATT CGCGCACCAA TGTTTCAGTA
    CAGCAAAAGG CTCAATTCCG TCTAAATGCC ACACGACCTG AGGAGGGCTG CATCCTGTGC
    TTCCAGTCAG ACTGCAAAAA TGACGGCTTC TGTCAATCTC CTTCAGATGA GTACGCCTGC
    ACCTGTCAGC CTGGATTCGA GGGCGATGAT TGCGGCACGG ACATCGACGA GTGTCTTAAC
    ACGGAATGCT TGAACAACGG CACCTGCATC AACCAGGTTG CAGCTTTCTT CTGCCAGTGT
    CAGCCAGGAT TCGAGGGTCA GCACTGTGAG CAAAACATCG ACGAGTGTGC GGATCAGCCG
    TGCCACAACG GTGGCAACTG CACGGATCTT ATCGCATCGT ACGTGTGCGA CTGCCCTGAG
    GACTATATGG GCCCGCAGTG CGACGTGCTG AAGCAAATGA CCTGCGAGAA CGAGCCATGT
    CGGAATGGAT CAACCTGCCA GAATGGATTC AATGCTTCTA CTGGCAATAA CTTTACATGT
    ACATGCGTGC CCGGCTTCGA GGGTCCACTG TGTGACATAC CCTTCTGTGA AATAACGCCT
    TGCGATAACG GTGGCCTCTG CCTGACCACT GGAGCGGTAC CGATGTGCAA ATGTAGTCTG
    GGATACACTG GTCGCCTGTG CGAGCAGGAC ATTAACGAGT GCGAATCGAA TCCATGCCAG
    AACGGTGGTC AGTGTAAGGA CCTCGTCGGC AGGTACGAGT GCGATTGCCA GGGCACCGGG
    TTCGAGGGCA TTCGCTGTGA AAATGACATC GACGAGTGCA ACATGGAGGG AGATTACTGC
    GGCGGATTGG GCCGGTGTTT CAACAAGCCC GGATCCTTCC AGTGCATCTG CCAGAAACCC
    TATTGCGGAG CCTACTGCAA CTTTACGGAT CCCTGCAACG CTACGGACCT CTGCTCAAAC
    GGCGGTCGCT GCGTAGAGTC CTGCGGCGCC AAACCGGACT ACTACTGCGA GTGTCCGGAA
    GGTTTCGCGG GAAAGAATTG CACAGCACCG ATTACGGCCA AGGAGGACGG GCCTTCGACC
    ACAGACATTG CCATCATTGT AATACCCGTA GTGGTGGTGC TGCTGCTGAT CGCGGGAGCC
    CTCCTGGGCA CCTTCCTGGT GATGGCCAGG AACAAGCGAG CAACCAGGGG CACCTATAGC
    CCGAGCGCGC AAGAGTACTG CAACCCACGG CTGGAAATGG ACAACGTACT GAAGCCACCG
    CCGGAAGAGC GACTAATTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08721
    Clone ID Related Accession (Same CDS sequence) NM_001260355.2
    Accession Version NM_001260355.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6762bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product crumbs, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260355.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGCTAAAA TCGCCAATGC GTCACTGTCG CAACAACAAA AACAGCGGCA AGCAGAAACA 
    GCAACGACCA CAACAACAAC AGTGGCGGCA TCAGTAGAAA CAGCAACAAC AACAGCAAGA
    AGTCGAGACC GCACGAAAAG CGCAGCTCAA ATTACGTCGC ATTTGCTCAA GCGCGCCATT
    TCAGTTTATT CGTCGCCTCA ATGGATACCG CTCTTCATAC TTATTTACTT AGCAACAGAT
    GTCGCCTCAG TGGCGGTGCC GACGAAGGAG GCGTACTTTA ATGGCTCCAC TTACCTCCGC
    CTCACCACGC CGATGCCCAT TTGGGATCAC TCGGCGATTA GTTTCCGCTC GTGCCGCGGC
    GGCGAGATCC TCGCCCAGCA GTACAACAAG AACTCCATTG TAATCTCAGT GCTCAATGAC
    TTTCTGCAAA TCTCACTGGC TGGACCCGCC GTCCATGGGC CCAACAACCG GCTGGATGTC
    AAGCTGCCCT ACCAACTGCT GGACAACCGC TGGCATACGC TGCAGTTCAA GTACGAGTAC
    GGAAATCTCT ACCTGCATGT GGATCGCGCG GCAAGCATAT TTGCCAACTC CACGTACAAC
    AGTCAGTTCC TGACTAACCA GGACATTGGC TACAAGGACG CCATCTTGAT ACTGGGAAAC
    TCCTTCTCCG GTTGCCTTCT GGATGGACCA GGTCTGCAAT TTGTGAACAA CTCGACGGTG
    CAGAATGTGG TCTTTGGTCA CTGTCCCTTG ACCCCGGGTC CCTGCAGCGA TCACGATCTT
    TTCACTCGAC TGCCAGACAA CTTTTGTCTG AATGATCCCT GTATGGGCCA TGGAACCTGC
    TCGTCCAGTC CAGAGGGATA CGAATGTCGG TGCACGGCAC GTTACTCGGG AAAAAATTGC
    CAGAAGGACA ATGGCTCGCC GTGTGCCAAA AATCCATGTG AGAACGGTGG TTCCTGCCTG
    GAGAATTCCC GAGGGGATTA CCAGTGCTTC TGTGATCCCA ACCACAGTGG TCAGCACTGC
    GAGACGGAGG TGAACATCCA CCCACTCTGC CAGACGAACC CCTGCCTGAA CAATGGAGCA
    TGTGTGGTGA TTGGCGGCAG TGGAGCACTC ACCTGCGAGT GTCCCAAAGG ATACGCTGGT
    GCCAGGTGCG AGGTGGACAC GGATGAGTGC GCCTCACAGC CGTGTCAGAA CAACGGAAGC
    TGCATAGACC GTATCAATGG ATTCAGCTGC GATTGCAGCG GCACCGGTTA CACGGGTGCG
    TTCTGCCAGA CGAATGTGGA TGAGTGCGAC AAGAATCCGT GCCTGAACGG CGGCAGATGC
    TTCGATACCT ACGGATGGTA CACCTGCCAG TGTCTGGATG GTTGGGGCGG CGAGATTTGT
    GATCGACCCA TGACATGCCA GACGCAGCAG TGTTTGAACG GTGGCACCTG CCTGGACAAG
    CCCATCGGCT TCCAGTGCCT CTGTCCGCCG GAGTACACTG GTGAACTGTG CCAAATCGCA
    CCCAGTTGCG CACAGCAGTG CCCCATCGAT TCGGAGTGCG TAGGCGGCAA ATGCGTTTGT
    AAGCCAGGCT CATCGGGCCC CATTGGTCAC TGCCTGCCAA CAACAACCAC ACCGACACCA
    GAACAAGAGC CAACAACAAC GCCAAGAACC ACACCAAACC CTAACCCAGC TATTCCCAAC
    ACCCTAACAA CAACAACCAA GATACCACCA ATAACAACCA GCCGAACACT GGTGGGAACG
    ACCACGGGTA GCAGGAGACC ACCACAACAG CCACTACAAT CTCCGACCCA ACGCTCGGCC
    TCCCTGAATG CATGTCCCCA AGAAAACTGC TTGAACGGTG GCACCTGCCT GGGCTATAGT
    GGCAATTATT CCTGTATTTG TGCTAGTGGA TACACAGGTT ACAACTGTCA AACGAGCACG
    GGCGATGGAG CTTCTGCTTT GGCCCTGACA CCCATCAACT GCAATGCCAC CAATGGAAAG
    TGTCTCAATG GAGGAACTTG CTCCATGAAC GGAACCCACT GCTATTGCGC CGTCGGCTAT
    TCGGGAGATC GTTGCGAGAA GGCCGAGAAC TGTTCGCCAC TGAACTGCCA GGAACCGATG
    GTATGTGTCC AGAATCAGTG CCTCTGCCCG GAGAACAAGG TGTGCAACCA GTGCGCCACA
    CAACCGTGCC AGAATGGCGG TGAATGTGTG GACCTACCGA ATGGAGACTA CGAGTGCAAG
    TGCACCCGTG GATGGACTGG ACGCACCTGT GGCAACGACG TGGACGAGTG CACCCTGCAT
    CCGAAGATCT GCGGCAATGG TATCTGCAAG AACGAGAAGG GATCGTACAA ATGCTACTGT
    ACGCCTGGAT TTACCGGAGT CCACTGCGAT TCCGATGTGG ACGAATGCCT TAGCTTTCCC
    TGCCTCAACG GAGCCACGTG CCACAACAAG ATAAATGCCT ACGAGTGCGT TTGCCAACCA
    GGATATGAGG GAGAGAACTG CGAAGTAGAT ATCGATGAGT GCGGCAGTAA TCCCTGCTCG
    AACGGATCCA CCTGCATCGA CAGGATCAAC AACTTCACCT GCAACTGCAT TCCGGGAATG
    ACGGGCCGCA TATGTGACAT CGACATTGAC GACTGTGTGG GAGATCCCTG CTTGAATGGT
    GGTCAGTGCA TCGACCAATT GGGTGGCTTC CGTTGTGACT GCAGTGGCAC CGGTTATGAG
    GGCGAGAATT GTGAGCTGAA CATAGACGAA TGCCTCTCGA ATCCGTGCAC GAATGGTGCC
    AAGTGCCTGG ACAGGGTTAA GGACTACTTC TGCGATTGCC ACAACGGCTA CAAGGGAAAG
    AACTGTGAGC AGGACATCAA CGAGTGCGAG AGTAATCCCT GCCAGTACAA TGGCAACTGC
    CTAGAGCGTT CGAACATAAC GCTTTACCAA ATGAGTAGGA TCACGGATCT GCCCAAGGTG
    TTTAGCCAGC CCTTTAGCTT TGAGAATGCC AGCGGCTACG AGTGCGTCTG TGTGCCCGGC
    ATCATTGGAA AGAACTGCGA GATCAACATT AATGAGTGCG ACAGTAATCC ATGCAGCAAG
    CACGGAAATT GCAATGACGG GATTGGAACC TATACCTGCG AATGCGAACC TGGCTTCGAG
    GGCACCCACT GTGAGATCAA CATAGATGAA TGTGATCGCT ATAATCCCTG CCAGAGGGGC
    ACTTGCTATG ACCAGATAGA CGACTACGAC TGCGACTGCG ATGCGAACTA TGGAGGCAAA
    AACTGTTCCG TTCTTCTCAA AGGCTGTGAC CAGAATCCTT GTTTAAATGG CGGTGCCTGC
    TTGCCGTATT TGATCAACGA GGTAACTCAT CTGTACAACT GCACCTGCGA GAACGGCTTC
    CAGGGCGATA AATGCGAGAA GACCACCACT CTGTCCATGG TGGCCACTAG CCTGATTTCG
    GTTACCACGG AACGCGAGGA AGGCTACGAC ATTAATCTTC AATTTAGGAC CACACTGCCC
    AACGGAGTTT TGGCTTTCGG AACCACCGGC GAAAAGAATG AACCAGTTAG CTATATTTTG
    GAGCTGATTA ATGGACGACT GAATCTACAT TCCTCGCTCC TAAATAAGTG GGAGGGCGTG
    TTTATTGGAT CGAAGCTGAA CGATAGCAAC TGGCACAAGG TGTTTGTGGC CATAAACACT
    TCGCATCTAG TCCTTTCGGC CAACGACGAG CAGGCTATTT TTCCGGTTGG CTCCTATGAA
    ACGGCCAATA ATAGCCAGCC TTCATTCCCG CGCACATACC TCGGAGGCAC CATTCCCAAT
    CTGAAGTCCT ATCTGCGCCA CCTCACCCAC CAACCATCAG CTTTTGTGGG CTGCATGCAA
    GATATAATGG TCAATGGTAA ATGGATCTTT CCCGATGAGC AGGATGCGAA TATCAGTTAT
    ACCAAACTGG AAAATGTCCA GAGCGGTTGT CCGCGCACCG AACAATGTAA ACCGAATCCT
    TGCCATTCGA ACGGTGAATG CACGGACCTT TGGCACACCT TCGCTTGTCA CTGTCCCAGA
    CCATTCTTTG GGCACACATG TCAGCACAAT ATGACTGCTG CCACTTTTGG CCACGAGAAC
    ACAACCCATT CGGCCGTGAT TGTGGAGACC ACAGATGTGG CTAGGCGAGC TATTAGATCC
    ATCCTAGACA TTTCCATGTT TATTCGTACA CGCGAGCCAA CTGGTCAAGT CTTTTACTTG
    GGCACTGATC CACGCAAAGC CCCCACTAAA AATATTGGCG ACTCATATGT GGCCGCCAAA
    TTACACGGCG GCGAGCTGCT GGTAAAGATG CAGTTCAGCG GTACTCCGGA GGCCTACACC
    GTTGGTGGCC AGAAATTGGA CAACGGCTAT AACCACCTGA TCGAGGTGGT GCGCAATCAA
    ACGCTCGTGC AGGTCAAGCT CAATGGCACC GAGTACTTCC GCAAGACGCT GTCCACGACG
    GGTCTGCTGG ACGCACAGGT GCTTTACTTG GGCGGACCTG CACCCACACG CGAGTCACTT
    CTGGGCGCGA CCACAGAACC AGGAATAATT CCAGTTCCGG GAGCAGGAAT ACCCATTGAA
    GACACAACAG TGCCCAAGGA GGCGGACGAC AGCAGGGACT ACTTTAAGGG CATAATCCAG
    GACGTGAAGG TTAGCAATGG CTCGCTCAAC CTGATTGTGG AGATGTATTC CCTAAACGTG
    ACAGATGTCC AAGTGAATGC CAAACCTCTG GGCGCTGTAA CCATCGATCG TGCCTCCGTG
    TTACCTGGTG AGGTGTCCGA TGATCTGTGC CGAAAGAATC CCTGCCTCCA TAATGCCGAG
    TGCAGGAACA CATGGAATGA CTACACTTGC AAATGCCCAA ATGGTTACAA GGGAAAGAAC
    TGCCAGGAGA TCGAATTTTG CCAACACGTT ACATGTCCGG GACAGAGCTT GTGCCAAAAT
    CTGGACGATG GCTATGAGTG TGTTACGAAC ACCACATTTA CTGGGCAGGA ACGCAGTCCT
    CTCGCCTTCT TCTACTTCCA GGAGCAGCAA TCTGACGATA TTGTCAGTGA AGCGTCTCCC
    AAGCAAACTC TTAAACCGGT AATTGATATT GCCTTCCGCA CTCGTGCTGG AGGTACTCTC
    CTGTACATAG ACAATGTGGA CGGATTCTTT GAAATTGGAG TAAACGGAGG ACGAGTGACC
    ATCACCTGGA AGCTAAGTGC ACTGCATTTT GGCGAGTCTG CACGCTTTGA GAAGGAGAAC
    ACTGACGGAG AATGGAGTCG CATTTACCTC AGGGCACACA ACAGCAAACT GGAGGGTGGA
    TGGAAGGGAT GGGAATCGAT GGTGGATCCG ACGCCAGCTT TTTCCACGGA CATCGACCAA
    GCGGCCTTTC AGTCCCTGAT CGCTACAAGT ACCCAGGTTT ACTTGGGTGG CATGCCAGAG
    TCGCGACAAG CACGAGGATC CACTTTGTCT GCCCAGCAGG GCTCTCAGTT CAAGGGCTGT
    GTGGGAGAGG CAAGGGTGGG CGATCTTTTA CTGCCCTACT TCTCCATGGC GGAACTGTAT
    TCGCGCACCA ATGTTTCAGT ACAGCAAAAG GCTCAATTCC GTCTAAATGC CACACGACCT
    GAGGAGGGCT GCATCCTGTG CTTCCAGTCA GACTGCAAAA ATGACGGCTT CTGTCAATCT
    CCTTCAGATG AGTACGCCTG CACCTGTCAG CCTGGATTCG AGGGCGATGA TTGCGGCACG
    GACATCGACG AGTGTCTTAA CACGGAATGC TTGAACAACG GCACCTGCAT CAACCAGGTT
    GCAGCTTTCT TCTGCCAGTG TCAGCCAGGA TTCGAGGGTC AGCACTGTGA GCAAAACATC
    GACGAGTGTG CGGATCAGCC GTGCCACAAC GGTGGCAACT GCACGGATCT TATCGCATCG
    TACGTGTGCG ACTGCCCTGA GGACTATATG GGCCCGCAGT GCGACGTGCT GAAGCAAATG
    ACCTGCGAGA ACGAGCCATG TCGGAATGGA TCAACCTGCC AGAATGGATT CAATGCTTCT
    ACTGGCAATA ACTTTACATG TACATGCGTG CCCGGCTTCG AGGGTCCACT GTGTGACATA
    CCCTTCTGTG AAATAACGCC TTGCGATAAC GGTGGCCTCT GCCTGACCAC TGGAGCGGTA
    CCGATGTGCA AATGTAGTCT GGGATACACT GGTCGCCTGT GCGAGCAGGA CATTAACGAG
    TGCGAATCGA ATCCATGCCA GAACGGTGGT CAGTGTAAGG ACCTCGTCGG CAGGTACGAG
    TGCGATTGCC AGGGCACCGG GTTCGAGGGC ATTCGCTGTG AAAATGACAT CGACGAGTGC
    AACATGGAGG GAGATTACTG CGGCGGATTG GGCCGGTGTT TCAACAAGCC CGGATCCTTC
    CAGTGCATCT GCCAGAAACC CTATTGCGGA GCCTACTGCA ACTTTACGGA TCCCTGCAAC
    GCTACGGACC TCTGCTCAAA CGGCGGTCGC TGCGTAGAGT CCTGCGGCGC CAAACCGGAC
    TACTACTGCG AGTGTCCGGA AGGTTTCGCG GGAAAGAATT GCACAGCACC GATTACGGCC
    AAGGAGGACG GGCCTTCGAC CACAGACATT GCCATCATTG TAATACCCGT AGTGGTGGTG
    CTGCTGCTGA TCGCGGGAGC CCTCCTGGGC ACCTTCCTGG TGATGGCCAG GAACAAGCGA
    GCAACCAGGG GCACCTATAG CCCGAGCGCG CAAGAGTACT GCAACCCACG GCTGGAAATG
    GACAACGTAC TGAAGCCACC GCCGGAAGAG CGACTAATTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001247284.1
    CDS550..7311
    Translation

    Target ORF information:

    RefSeq Version NM_001260355.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster crumbs (crb), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001260355.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    ATGGCTAAAA TCGCCAATGC GTCACTGTCG CAACAACAAA AACAGCGGCA AGCAGAAACA 
    GCAACGACCA CAACAACAAC AGTGGCGGCA TCAGTAGAAA CAGCAACAAC AACAGCAAGA
    AGTCGAGACC GCACGAAAAG CGCAGCTCAA ATTACGTCGC ATTTGCTCAA GCGCGCCATT
    TCAGTTTATT CGTCGCCTCA ATGGATACCG CTCTTCATAC TTATTTACTT AGCAACAGAT
    GTCGCCTCAG TGGCGGTGCC GACGAAGGAG GCGTACTTTA ATGGCTCCAC TTACCTCCGC
    CTCACCACGC CGATGCCCAT TTGGGATCAC TCGGCGATTA GTTTCCGCTC GTGCCGCGGC
    GGCGAGATCC TCGCCCAGCA GTACAACAAG AACTCCATTG TAATCTCAGT GCTCAATGAC
    TTTCTGCAAA TCTCACTGGC TGGACCCGCC GTCCATGGGC CCAACAACCG GCTGGATGTC
    AAGCTGCCCT ACCAACTGCT GGACAACCGC TGGCATACGC TGCAGTTCAA GTACGAGTAC
    GGAAATCTCT ACCTGCATGT GGATCGCGCG GCAAGCATAT TTGCCAACTC CACGTACAAC
    AGTCAGTTCC TGACTAACCA GGACATTGGC TACAAGGACG CCATCTTGAT ACTGGGAAAC
    TCCTTCTCCG GTTGCCTTCT GGATGGACCA GGTCTGCAAT TTGTGAACAA CTCGACGGTG
    CAGAATGTGG TCTTTGGTCA CTGTCCCTTG ACCCCGGGTC CCTGCAGCGA TCACGATCTT
    TTCACTCGAC TGCCAGACAA CTTTTGTCTG AATGATCCCT GTATGGGCCA TGGAACCTGC
    TCGTCCAGTC CAGAGGGATA CGAATGTCGG TGCACGGCAC GTTACTCGGG AAAAAATTGC
    CAGAAGGACA ATGGCTCGCC GTGTGCCAAA AATCCATGTG AGAACGGTGG TTCCTGCCTG
    GAGAATTCCC GAGGGGATTA CCAGTGCTTC TGTGATCCCA ACCACAGTGG TCAGCACTGC
    GAGACGGAGG TGAACATCCA CCCACTCTGC CAGACGAACC CCTGCCTGAA CAATGGAGCA
    TGTGTGGTGA TTGGCGGCAG TGGAGCACTC ACCTGCGAGT GTCCCAAAGG ATACGCTGGT
    GCCAGGTGCG AGGTGGACAC GGATGAGTGC GCCTCACAGC CGTGTCAGAA CAACGGAAGC
    TGCATAGACC GTATCAATGG ATTCAGCTGC GATTGCAGCG GCACCGGTTA CACGGGTGCG
    TTCTGCCAGA CGAATGTGGA TGAGTGCGAC AAGAATCCGT GCCTGAACGG CGGCAGATGC
    TTCGATACCT ACGGATGGTA CACCTGCCAG TGTCTGGATG GTTGGGGCGG CGAGATTTGT
    GATCGACCCA TGACATGCCA GACGCAGCAG TGTTTGAACG GTGGCACCTG CCTGGACAAG
    CCCATCGGCT TCCAGTGCCT CTGTCCGCCG GAGTACACTG GTGAACTGTG CCAAATCGCA
    CCCAGTTGCG CACAGCAGTG CCCCATCGAT TCGGAGTGCG TAGGCGGCAA ATGCGTTTGT
    AAGCCAGGCT CATCGGGCCC CATTGGTCAC TGCCTGCCAA CAACAACCAC ACCGACACCA
    GAACAAGAGC CAACAACAAC GCCAAGAACC ACACCAAACC CTAACCCAGC TATTCCCAAC
    ACCCTAACAA CAACAACCAA GATACCACCA ATAACAACCA GCCGAACACT GGTGGGAACG
    ACCACGGGTA GCAGGAGACC ACCACAACAG CCACTACAAT CTCCGACCCA ACGCTCGGCC
    TCCCTGAATG CATGTCCCCA AGAAAACTGC TTGAACGGTG GCACCTGCCT GGGCTATAGT
    GGCAATTATT CCTGTATTTG TGCTAGTGGA TACACAGGTT ACAACTGTCA AACGAGCACG
    GGCGATGGAG CTTCTGCTTT GGCCCTGACA CCCATCAACT GCAATGCCAC CAATGGAAAG
    TGTCTCAATG GAGGAACTTG CTCCATGAAC GGAACCCACT GCTATTGCGC CGTCGGCTAT
    TCGGGAGATC GTTGCGAGAA GGCCGAGAAC TGTTCGCCAC TGAACTGCCA GGAACCGATG
    GTATGTGTCC AGAATCAGTG CCTCTGCCCG GAGAACAAGG TGTGCAACCA GTGCGCCACA
    CAACCGTGCC AGAATGGCGG TGAATGTGTG GACCTACCGA ATGGAGACTA CGAGTGCAAG
    TGCACCCGTG GATGGACTGG ACGCACCTGT GGCAACGACG TGGACGAGTG CACCCTGCAT
    CCGAAGATCT GCGGCAATGG TATCTGCAAG AACGAGAAGG GATCGTACAA ATGCTACTGT
    ACGCCTGGAT TTACCGGAGT CCACTGCGAT TCCGATGTGG ACGAATGCCT TAGCTTTCCC
    TGCCTCAACG GAGCCACGTG CCACAACAAG ATAAATGCCT ACGAGTGCGT TTGCCAACCA
    GGATATGAGG GAGAGAACTG CGAAGTAGAT ATCGATGAGT GCGGCAGTAA TCCCTGCTCG
    AACGGATCCA CCTGCATCGA CAGGATCAAC AACTTCACCT GCAACTGCAT TCCGGGAATG
    ACGGGCCGCA TATGTGACAT CGACATTGAC GACTGTGTGG GAGATCCCTG CTTGAATGGT
    GGTCAGTGCA TCGACCAATT GGGTGGCTTC CGTTGTGACT GCAGTGGCAC CGGTTATGAG
    GGCGAGAATT GTGAGCTGAA CATAGACGAA TGCCTCTCGA ATCCGTGCAC GAATGGTGCC
    AAGTGCCTGG ACAGGGTTAA GGACTACTTC TGCGATTGCC ACAACGGCTA CAAGGGAAAG
    AACTGTGAGC AGGACATCAA CGAGTGCGAG AGTAATCCCT GCCAGTACAA TGGCAACTGC
    CTAGAGCGTT CGAACATAAC GCTTTACCAA ATGAGTAGGA TCACGGATCT GCCCAAGGTG
    TTTAGCCAGC CCTTTAGCTT TGAGAATGCC AGCGGCTACG AGTGCGTCTG TGTGCCCGGC
    ATCATTGGAA AGAACTGCGA GATCAACATT AATGAGTGCG ACAGTAATCC ATGCAGCAAG
    CACGGAAATT GCAATGACGG GATTGGAACC TATACCTGCG AATGCGAACC TGGCTTCGAG
    GGCACCCACT GTGAGATCAA CATAGATGAA TGTGATCGCT ATAATCCCTG CCAGAGGGGC
    ACTTGCTATG ACCAGATAGA CGACTACGAC TGCGACTGCG ATGCGAACTA TGGAGGCAAA
    AACTGTTCCG TTCTTCTCAA AGGCTGTGAC CAGAATCCTT GTTTAAATGG CGGTGCCTGC
    TTGCCGTATT TGATCAACGA GGTAACTCAT CTGTACAACT GCACCTGCGA GAACGGCTTC
    CAGGGCGATA AATGCGAGAA GACCACCACT CTGTCCATGG TGGCCACTAG CCTGATTTCG
    GTTACCACGG AACGCGAGGA AGGCTACGAC ATTAATCTTC AATTTAGGAC CACACTGCCC
    AACGGAGTTT TGGCTTTCGG AACCACCGGC GAAAAGAATG AACCAGTTAG CTATATTTTG
    GAGCTGATTA ATGGACGACT GAATCTACAT TCCTCGCTCC TAAATAAGTG GGAGGGCGTG
    TTTATTGGAT CGAAGCTGAA CGATAGCAAC TGGCACAAGG TGTTTGTGGC CATAAACACT
    TCGCATCTAG TCCTTTCGGC CAACGACGAG CAGGCTATTT TTCCGGTTGG CTCCTATGAA
    ACGGCCAATA ATAGCCAGCC TTCATTCCCG CGCACATACC TCGGAGGCAC CATTCCCAAT
    CTGAAGTCCT ATCTGCGCCA CCTCACCCAC CAACCATCAG CTTTTGTGGG CTGCATGCAA
    GATATAATGG TCAATGGTAA ATGGATCTTT CCCGATGAGC AGGATGCGAA TATCAGTTAT
    ACCAAACTGG AAAATGTCCA GAGCGGTTGT CCGCGCACCG AACAATGTAA ACCGAATCCT
    TGCCATTCGA ACGGTGAATG CACGGACCTT TGGCACACCT TCGCTTGTCA CTGTCCCAGA
    CCATTCTTTG GGCACACATG TCAGCACAAT ATGACTGCTG CCACTTTTGG CCACGAGAAC
    ACAACCCATT CGGCCGTGAT TGTGGAGACC ACAGATGTGG CTAGGCGAGC TATTAGATCC
    ATCCTAGACA TTTCCATGTT TATTCGTACA CGCGAGCCAA CTGGTCAAGT CTTTTACTTG
    GGCACTGATC CACGCAAAGC CCCCACTAAA AATATTGGCG ACTCATATGT GGCCGCCAAA
    TTACACGGCG GCGAGCTGCT GGTAAAGATG CAGTTCAGCG GTACTCCGGA GGCCTACACC
    GTTGGTGGCC AGAAATTGGA CAACGGCTAT AACCACCTGA TCGAGGTGGT GCGCAATCAA
    ACGCTCGTGC AGGTCAAGCT CAATGGCACC GAGTACTTCC GCAAGACGCT GTCCACGACG
    GGTCTGCTGG ACGCACAGGT GCTTTACTTG GGCGGACCTG CACCCACACG CGAGTCACTT
    CTGGGCGCGA CCACAGAACC AGGAATAATT CCAGTTCCGG GAGCAGGAAT ACCCATTGAA
    GACACAACAG TGCCCAAGGA GGCGGACGAC AGCAGGGACT ACTTTAAGGG CATAATCCAG
    GACGTGAAGG TTAGCAATGG CTCGCTCAAC CTGATTGTGG AGATGTATTC CCTAAACGTG
    ACAGATGTCC AAGTGAATGC CAAACCTCTG GGCGCTGTAA CCATCGATCG TGCCTCCGTG
    TTACCTGGTG AGGTGTCCGA TGATCTGTGC CGAAAGAATC CCTGCCTCCA TAATGCCGAG
    TGCAGGAACA CATGGAATGA CTACACTTGC AAATGCCCAA ATGGTTACAA GGGAAAGAAC
    TGCCAGGAGA TCGAATTTTG CCAACACGTT ACATGTCCGG GACAGAGCTT GTGCCAAAAT
    CTGGACGATG GCTATGAGTG TGTTACGAAC ACCACATTTA CTGGGCAGGA ACGCAGTCCT
    CTCGCCTTCT TCTACTTCCA GGAGCAGCAA TCTGACGATA TTGTCAGTGA AGCGTCTCCC
    AAGCAAACTC TTAAACCGGT AATTGATATT GCCTTCCGCA CTCGTGCTGG AGGTACTCTC
    CTGTACATAG ACAATGTGGA CGGATTCTTT GAAATTGGAG TAAACGGAGG ACGAGTGACC
    ATCACCTGGA AGCTAAGTGC ACTGCATTTT GGCGAGTCTG CACGCTTTGA GAAGGAGAAC
    ACTGACGGAG AATGGAGTCG CATTTACCTC AGGGCACACA ACAGCAAACT GGAGGGTGGA
    TGGAAGGGAT GGGAATCGAT GGTGGATCCG ACGCCAGCTT TTTCCACGGA CATCGACCAA
    GCGGCCTTTC AGTCCCTGAT CGCTACAAGT ACCCAGGTTT ACTTGGGTGG CATGCCAGAG
    TCGCGACAAG CACGAGGATC CACTTTGTCT GCCCAGCAGG GCTCTCAGTT CAAGGGCTGT
    GTGGGAGAGG CAAGGGTGGG CGATCTTTTA CTGCCCTACT TCTCCATGGC GGAACTGTAT
    TCGCGCACCA ATGTTTCAGT ACAGCAAAAG GCTCAATTCC GTCTAAATGC CACACGACCT
    GAGGAGGGCT GCATCCTGTG CTTCCAGTCA GACTGCAAAA ATGACGGCTT CTGTCAATCT
    CCTTCAGATG AGTACGCCTG CACCTGTCAG CCTGGATTCG AGGGCGATGA TTGCGGCACG
    GACATCGACG AGTGTCTTAA CACGGAATGC TTGAACAACG GCACCTGCAT CAACCAGGTT
    GCAGCTTTCT TCTGCCAGTG TCAGCCAGGA TTCGAGGGTC AGCACTGTGA GCAAAACATC
    GACGAGTGTG CGGATCAGCC GTGCCACAAC GGTGGCAACT GCACGGATCT TATCGCATCG
    TACGTGTGCG ACTGCCCTGA GGACTATATG GGCCCGCAGT GCGACGTGCT GAAGCAAATG
    ACCTGCGAGA ACGAGCCATG TCGGAATGGA TCAACCTGCC AGAATGGATT CAATGCTTCT
    ACTGGCAATA ACTTTACATG TACATGCGTG CCCGGCTTCG AGGGTCCACT GTGTGACATA
    CCCTTCTGTG AAATAACGCC TTGCGATAAC GGTGGCCTCT GCCTGACCAC TGGAGCGGTA
    CCGATGTGCA AATGTAGTCT GGGATACACT GGTCGCCTGT GCGAGCAGGA CATTAACGAG
    TGCGAATCGA ATCCATGCCA GAACGGTGGT CAGTGTAAGG ACCTCGTCGG CAGGTACGAG
    TGCGATTGCC AGGGCACCGG GTTCGAGGGC ATTCGCTGTG AAAATGACAT CGACGAGTGC
    AACATGGAGG GAGATTACTG CGGCGGATTG GGCCGGTGTT TCAACAAGCC CGGATCCTTC
    CAGTGCATCT GCCAGAAACC CTATTGCGGA GCCTACTGCA ACTTTACGGA TCCCTGCAAC
    GCTACGGACC TCTGCTCAAA CGGCGGTCGC TGCGTAGAGT CCTGCGGCGC CAAACCGGAC
    TACTACTGCG AGTGTCCGGA AGGTTTCGCG GGAAAGAATT GCACAGCACC GATTACGGCC
    AAGGAGGACG GGCCTTCGAC CACAGACATT GCCATCATTG TAATACCCGT AGTGGTGGTG
    CTGCTGCTGA TCGCGGGAGC CCTCCTGGGC ACCTTCCTGG TGATGGCCAG GAACAAGCGA
    GCAACCAGGG GCACCTATAG CCCGAGCGCG CAAGAGTACT GCAACCCACG GCTGGAAATG
    GACAACGTAC TGAAGCCACC GCCGGAAGAG CGACTAATTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08722
    Clone ID Related Accession (Same CDS sequence) NM_001275980.1
    Accession Version NM_001275980.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6612bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product crumbs, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGCTAAAA TCGCCAATGC GTCACTGTCG CAACAACAAA AACAGCGGCA AGCAGAAACA 
    GCAACGACCA CAACAACAAC AGTGGCGGCA TCAGTAGAAA CAGCAACAAC AACAGCAAGA
    AGTCGAGACC GCACGAAAAG CGCAGCTCAA ATTACGTCGC ATTTGCTCAA GCGCGCCATT
    TCAGTTTATT CGTCGCCTCA ATGGATACCG CTCTTCATAC TTATTTACTT AGCAACAGAT
    GTCGCCTCAG TGGCGGTGCC GACGAAGGAG GCGTACTTTA ATGGCTCCAC TTACCTCCGC
    CTCACCACGC CGATGCCCAT TTGGGATCAC TCGGCGATTA GTTTCCGCTC GTGCCGCGGC
    GGCGAGATCC TCGCCCAGCA GTACAACAAG AACTCCATTG TAATCTCAGT GCTCAATGAC
    TTTCTGCAAA TCTCACTGGC TGGACCCGCC GTCCATGGGC CCAACAACCG GCTGGATGTC
    AAGCTGCCCT ACCAACTGCT GGACAACCGC TGGCATACGC TGCAGTTCAA GTACGAGTAC
    GGAAATCTCT ACCTGCATGT GGATCGCGCG GCAAGCATAT TTGCCAACTC CACGTACAAC
    AGTCAGTTCC TGACTAACCA GGACATTGGC TACAAGGACG CCATCTTGAT ACTGGGAAAC
    TCCTTCTCCG GTTGCCTTCT GGATGGACCA GGTCTGCAAT TTGTGAACAA CTCGACGGTG
    CAGAATGTGG TCTTTGGTCA CTGTCCCTTG ACCCCGGGTC CCTGCAGCGA TCACGATCTT
    TTCACTCGAC TGCCAGACAA CTTTTGTCTG AATGATCCCT GTATGGGCCA TGGAACCTGC
    TCGTCCAGTC CAGAGGGATA CGAATGTCGG TGCACGGCAC GTTACTCGGG AAAAAATTGC
    CAGAAGGACA ATGGCTCGCC GTGTGCCAAA AATCCATGTG AGAACGGTGG TTCCTGCCTG
    GAGAATTCCC GAGGGGATTA CCAGTGCTTC TGTGATCCCA ACCACAGTGG TCAGCACTGC
    GAGACGGAGG TGAACATCCA CCCACTCTGC CAGACGAACC CCTGCCTGAA CAATGGAGCA
    TGTGTGGTGA TTGGCGGCAG TGGAGCACTC ACCTGCGAGT GTCCCAAAGG ATACGCTGGT
    GCCAGGTGCG AGGTGGACAC GGATGAGTGC GCCTCACAGC CGTGTCAGAA CAACGGAAGC
    TGCATAGACC GTATCAATGG ATTCAGCTGC GATTGCAGCG GCACCGGTTA CACGGGTGCG
    TTCTGCCAGA CGAATGTGGA TGAGTGCGAC AAGAATCCGT GCCTGAACGG CGGCAGATGC
    TTCGATACCT ACGGATGGTA CACCTGCCAG TGTCTGGATG GTTGGGGCGG CGAGATTTGT
    GATCGACCCA TGACATGCCA GACGCAGCAG TGTTTGAACG GTGGCACCTG CCTGGACAAG
    CCCATCGGCT TCCAGTGCCT CTGTCCGCCG GAGTACACTG GTGAACTGTG CCAAATCGCA
    CCCAGTTGCG CACAGCAGTG CCCCATCGAT TCGGAGTGCG TAGGCGGCAA ATGCGTTTGT
    AAGCCAGGCT CATCGGCTTC CGACATGGAG CCGCTGACTC CGCTCGAGCT GGACATTCTC
    GACGCCACGC TGTGCCCCAG CGAAAAGAAG AAGCGCTACA TATCGCCCGA GTGGCTCAAA
    CGCAAGCGTT GTGAGCTAAA ACTAAGTTAC AACTGTCAAA CGAGCACGGG CGATGGAGCT
    TCTGCTTTGG CCCTGACACC CATCAACTGC AATGCCACCA ATGGAAAGTG TCTCAATGGA
    GGAACTTGCT CCATGAACGG AACCCACTGC TATTGCGCCG TCGGCTATTC GGGAGATCGT
    TGCGAGAAGG CCGAGAACTG TTCGCCACTG AACTGCCAGG AACCGATGGT ATGTGTCCAG
    AATCAGTGCC TCTGCCCGGA GAACAAGGTG TGCAACCAGT GCGCCACACA ACCGTGCCAG
    AATGGCGGTG AATGTGTGGA CCTACCGAAT GGAGACTACG AGTGCAAGTG CACCCGTGGA
    TGGACTGGAC GCACCTGTGG CAACGACGTG GACGAGTGCA CCCTGCATCC GAAGATCTGC
    GGCAATGGTA TCTGCAAGAA CGAGAAGGGA TCGTACAAAT GCTACTGTAC GCCTGGATTT
    ACCGGAGTCC ACTGCGATTC CGATGTGGAC GAATGCCTTA GCTTTCCCTG CCTCAACGGA
    GCCACGTGCC ACAACAAGTT GTTTTTCTTT AAATTCGGAA AGCATCGGAT TTTGAGACAA
    ATAAATGCCT ACGAGTGCGT TTGCCAACCA GGATATGAGG GAGAGAACTG CGAAGTAGAT
    ATCGATGAGT GCGGCAGTAA TCCCTGCTCG AACGGATCCA CCTGCATCGA CAGGATCAAC
    AACTTCACCT GCAACTGCAT TCCGGGAATG ACGGGCCGCA TATGTGACAT CGACATTGAC
    GACTGTGTGG GAGATCCCTG CTTGAATGGT GGTCAGTGCA TCGACCAATT GGGTGGCTTC
    CGTTGTGACT GCAGTGGCAC CGGTTATGAG GGCGAGAATT GTGAGCTGAA CATAGACGAA
    TGCCTCTCGA ATCCGTGCAC GAATGGTGCC AAGTGCCTGG ACAGGGTTAA GGACTACTTC
    TGCGATTGCC ACAACGGCTA CAAGGGAAAG AACTGTGAGC AGGACATCAA CGAGTGCGAG
    AGTAATCCCT GCCAGTACAA TGGCAACTGC CTAGAGCGTT CGAACATAAC GCTTTACCAA
    ATGAGTAGGA TCACGGATCT GCCCAAGGTG TTTAGCCAGC CCTTTAGCTT TGAGAATGCC
    AGCGGCTACG AGTGCGTCTG TGTGCCCGGC ATCATTGGAA AGAACTGCGA GATCAACATT
    AATGAGTGCG ACAGTAATCC ATGCAGCAAG CACGGAAATT GCAATGACGG GATTGGAACC
    TATACCTGCG AATGCGAACC TGGCTTCGAG GGCACCCACT GTGAGATCAA CATAGATGAA
    TGTGATCGCT ATAATCCCTG CCAGAGGGGC ACTTGCTATG ACCAGATAGA CGACTACGAC
    TGCGACTGCG ATGCGAACTA TGGAGGCAAA AACTGTTCCG TTCTTCTCAA AGGCTGTGAC
    CAGAATCCTT GTTTAAATGG CGGTGCCTGC TTGCCGTATT TGATCAACGA GGTAACTCAT
    CTGTACAACT GCACCTGCGA GAACGGCTTC CAGGGCGATA AATGCGAGAA GACCACCACT
    CTGTCCATGG TGGCCACTAG CCTGATTTCG GTTACCACGG AACGCGAGGA AGGCTACGAC
    ATTAATCTTC AATTTAGGAC CACACTGCCC AACGGAGTTT TGGCTTTCGG AACCACCGGC
    GAAAAGAATG AACCAGTTAG CTATATTTTG GAGCTGATTA ATGGACGACT GAATCTACAT
    TCCTCGCTCC TAAATAAGTG GGAGGGCGTG TTTATTGGAT CGAAGCTGAA CGATAGCAAC
    TGGCACAAGG TGTTTGTGGC CATAAACACT TCGCATCTAG TCCTTTCGGC CAACGACGAG
    CAGGCTATTT TTCCGGTTGG CTCCTATGAA ACGGCCAATA ATAGCCAGCC TTCATTCCCG
    CGCACATACC TCGGAGGCAC CATTCCCAAT CTGAAGTCCT ATCTGCGCCA CCTCACCCAC
    CAACCATCAG CTTTTGTGGG CTGCATGCAA GATATAATGG TCAATGGTAA ATGGATCTTT
    CCCGATGAGC AGGATGCGAA TATCAGTTAT ACCAAACTGG AAAATGTCCA GAGCGGTTGT
    CCGCGCACCG AACAATGTAA ACCGAATCCT TGCCATTCGA ACGGTGAATG CACGGACCTT
    TGGCACACCT TCGCTTGTCA CTGTCCCAGA CCATTCTTTG GGCACACATG TCAGCACAAT
    ATGACTGCTG CCACTTTTGG CCACGAGAAC ACAACCCATT CGGCCGTGAT TGTGGAGACC
    ACAGATGTGG CTAGGCGAGC TATTAGATCC ATCCTAGACA TTTCCATGTT TATTCGTACA
    CGCGAGCCAA CTGGTCAAGT CTTTTACTTG GGCACTGATC CACGCAAAGC CCCCACTAAA
    AATATTGGCG ACTCATATGT GGCCGCCAAA TTACACGGCG GCGAGCTGCT GGTAAAGATG
    CAGTTCAGCG GTACTCCGGA GGCCTACACC GTTGGTGGCC AGAAATTGGA CAACGGCTAT
    AACCACCTGA TCGAGGTGGT GCGCAATCAA ACGCTCGTGC AGGTCAAGCT CAATGGCACC
    GAGTACTTCC GCAAGACGCT GTCCACGACG GGTCTGCTGG ACGCACAGGT GCTTTACTTG
    GGCGGACCTG CACCCACACG CGAGTCACTT CTGGGCGCGA CCACAGAACC AGGAATAATT
    CCAGTTCCGG GAGCAGGAAT ACCCATTGAA GACACAACAG TGCCCAAGGA GGCGGACGAC
    AGCAGGGACT ACTTTAAGGG CATAATCCAG GACGTGAAGG TTAGCAATGG CTCGCTCAAC
    CTGATTGTGG AGATGTATTC CCTAAACGTG ACAGATGTCC AAGTGAATGC CAAACCTCTG
    GGCGCTGTAA CCATCGATCG TGCCTCCGTG TTACCTGGTG AGGTGTCCGA TGATCTGTGC
    CGAAAGAATC CCTGCCTCCA TAATGCCGAG TGCAGGAACA CATGGAATGA CTACACTTGC
    AAATGCCCAA ATGGTTACAA GGGAAAGAAC TGCCAGGAGA TCGAATTTTG CCAACACGTT
    ACATGTCCGG GACAGAGCTT GTGCCAAAAT CTGGACGATG GCTATGAGTG TGTTACGAAC
    ACCACATTTA CTGGGCAGGA ACGCAGTCCT CTCGCCTTCT TCTACTTCCA GGAGCAGCAA
    TCTGACGATA TTGTCAGTGA AGCGTCTCCC AAGCAAACTC TTAAACCGGT AATTGATATT
    GCCTTCCGCA CTCGTGCTGG AGGTACTCTC CTGTACATAG ACAATGTGGA CGGATTCTTT
    GAAATTGGAG TAAACGGAGG ACGAGTGACC ATCACCTGGA AGCTAAGTGC ACTGCATTTT
    GGCGAGTCTG CACGCTTTGA GAAGGAGAAC ACTGACGGAG AATGGAGTCG CATTTACCTC
    AGGGCACACA ACAGCAAACT GGAGGGTGGA TGGAAGGGAT GGGAATCGAT GGTGGATCCG
    ACGCCAGCTT TTTCCACGGA CATCGACCAA GCGGCCTTTC AGTCCCTGAT CGCTACAAGT
    ACCCAGGTTT ACTTGGGTGG CATGCCAGAG TCGCGACAAG CACGAGGATC CACTTTGTCT
    GCCCAGCAGG GCTCTCAGTT CAAGGGCTGT GTGGGAGAGG CAAGGGTGGG CGATCTTTTA
    CTGCCCTACT TCTCCATGGC GGAACTGTAT TCGCGCACCA ATGTTTCAGT ACAGCAAAAG
    GCTCAATTCC GTCTAAATGC CACACGACCT GAGGAGGGCT GCATCCTGTG CTTCCAGTCA
    GACTGCAAAA ATGACGGCTT CTGTCAATCT CCTTCAGATG AGTACGCCTG CACCTGTCAG
    CCTGGATTCG AGGGCGATGA TTGCGGCACG GACATCGACG AGTGTCTTAA CACGGAATGC
    TTGAACAACG GCACCTGCAT CAACCAGGTT GCAGCTTTCT TCTGCCAGTG TCAGCCAGGA
    TTCGAGGGTC AGCACTGTGA GCAAAACATC GACGAGTGTG CGGATCAGCC GTGCCACAAC
    GGTGGCAACT GCACGGATCT TATCGCATCG TACGTGTGCG ACTGCCCTGA GGACTATATG
    GGCCCGCAGT GCGACGTGCT GAAGCAAATG ACCTGCGAGA ACGAGCCATG TCGGAATGGA
    TCAACCTGCC AGAATGGATT CAATGCTTCT ACTGGCAATA ACTTTACATG TACATGCGTG
    CCCGGCTTCG AGGGTCCACT GTGTGACATA CCCTTCTGTG AAATAACGCC TTGCGATAAC
    GGTGGCCTCT GCCTGACCAC TGGAGCGGTA CCGATGTGCA AATGTAGTCT GGGATACACT
    GGTCGCCTGT GCGAGCAGGA CATTAACGAG TGCGAATCGA ATCCATGCCA GAACGGTGGT
    CAGTGTAAGG ACCTCGTCGG CAGGTACGAG TGCGATTGCC AGGGCACCGG GTTCGAGGGC
    ATTCGCTGTG AAAATGACAT CGACGAGTGC AACATGGAGG GAGATTACTG CGGCGGATTG
    GGCCGGTGTT TCAACAAGCC CGGATCCTTC CAGTGCATCT GCCAGAAACC CTATTGCGGA
    GCCTACTGCA ACTTTACGGA TCCCTGCAAC GCTACGGACC TCTGCTCAAA CGGCGGTCGC
    TGCGTAGAGT CCTGCGGCGC CAAACCGGAC TACTACTGCG AGTGTCCGGA AGGTTTCGCG
    GGAAAGAATT GCACAGCACC GATTACGGCC AAGGAGGACG GGCCTTCGAC CACAGACATT
    GCCATCATTG TAATACCCGT AGTGGTGGTG CTGCTGCTGA TCGCGGGAGC CCTCCTGGGC
    ACCTTCCTGG TGATGGCCAG GAACAAGCGA GCAACCAGGG GCACCTATAG CCCGAGCGCG
    CAAGAGTACT GCAACCCACG GCTGGAAATG GACAACGTAC TGAAGCCACC GCCGGAAGAG
    CGACTAATTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001262909.1
    CDS550..7161
    Translation

    Target ORF information:

    RefSeq Version NM_001275980.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster crumbs (crb), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001275980.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGCTAAAA TCGCCAATGC GTCACTGTCG CAACAACAAA AACAGCGGCA AGCAGAAACA 
    GCAACGACCA CAACAACAAC AGTGGCGGCA TCAGTAGAAA CAGCAACAAC AACAGCAAGA
    AGTCGAGACC GCACGAAAAG CGCAGCTCAA ATTACGTCGC ATTTGCTCAA GCGCGCCATT
    TCAGTTTATT CGTCGCCTCA ATGGATACCG CTCTTCATAC TTATTTACTT AGCAACAGAT
    GTCGCCTCAG TGGCGGTGCC GACGAAGGAG GCGTACTTTA ATGGCTCCAC TTACCTCCGC
    CTCACCACGC CGATGCCCAT TTGGGATCAC TCGGCGATTA GTTTCCGCTC GTGCCGCGGC
    GGCGAGATCC TCGCCCAGCA GTACAACAAG AACTCCATTG TAATCTCAGT GCTCAATGAC
    TTTCTGCAAA TCTCACTGGC TGGACCCGCC GTCCATGGGC CCAACAACCG GCTGGATGTC
    AAGCTGCCCT ACCAACTGCT GGACAACCGC TGGCATACGC TGCAGTTCAA GTACGAGTAC
    GGAAATCTCT ACCTGCATGT GGATCGCGCG GCAAGCATAT TTGCCAACTC CACGTACAAC
    AGTCAGTTCC TGACTAACCA GGACATTGGC TACAAGGACG CCATCTTGAT ACTGGGAAAC
    TCCTTCTCCG GTTGCCTTCT GGATGGACCA GGTCTGCAAT TTGTGAACAA CTCGACGGTG
    CAGAATGTGG TCTTTGGTCA CTGTCCCTTG ACCCCGGGTC CCTGCAGCGA TCACGATCTT
    TTCACTCGAC TGCCAGACAA CTTTTGTCTG AATGATCCCT GTATGGGCCA TGGAACCTGC
    TCGTCCAGTC CAGAGGGATA CGAATGTCGG TGCACGGCAC GTTACTCGGG AAAAAATTGC
    CAGAAGGACA ATGGCTCGCC GTGTGCCAAA AATCCATGTG AGAACGGTGG TTCCTGCCTG
    GAGAATTCCC GAGGGGATTA CCAGTGCTTC TGTGATCCCA ACCACAGTGG TCAGCACTGC
    GAGACGGAGG TGAACATCCA CCCACTCTGC CAGACGAACC CCTGCCTGAA CAATGGAGCA
    TGTGTGGTGA TTGGCGGCAG TGGAGCACTC ACCTGCGAGT GTCCCAAAGG ATACGCTGGT
    GCCAGGTGCG AGGTGGACAC GGATGAGTGC GCCTCACAGC CGTGTCAGAA CAACGGAAGC
    TGCATAGACC GTATCAATGG ATTCAGCTGC GATTGCAGCG GCACCGGTTA CACGGGTGCG
    TTCTGCCAGA CGAATGTGGA TGAGTGCGAC AAGAATCCGT GCCTGAACGG CGGCAGATGC
    TTCGATACCT ACGGATGGTA CACCTGCCAG TGTCTGGATG GTTGGGGCGG CGAGATTTGT
    GATCGACCCA TGACATGCCA GACGCAGCAG TGTTTGAACG GTGGCACCTG CCTGGACAAG
    CCCATCGGCT TCCAGTGCCT CTGTCCGCCG GAGTACACTG GTGAACTGTG CCAAATCGCA
    CCCAGTTGCG CACAGCAGTG CCCCATCGAT TCGGAGTGCG TAGGCGGCAA ATGCGTTTGT
    AAGCCAGGCT CATCGGCTTC CGACATGGAG CCGCTGACTC CGCTCGAGCT GGACATTCTC
    GACGCCACGC TGTGCCCCAG CGAAAAGAAG AAGCGCTACA TATCGCCCGA GTGGCTCAAA
    CGCAAGCGTT GTGAGCTAAA ACTAAGTTAC AACTGTCAAA CGAGCACGGG CGATGGAGCT
    TCTGCTTTGG CCCTGACACC CATCAACTGC AATGCCACCA ATGGAAAGTG TCTCAATGGA
    GGAACTTGCT CCATGAACGG AACCCACTGC TATTGCGCCG TCGGCTATTC GGGAGATCGT
    TGCGAGAAGG CCGAGAACTG TTCGCCACTG AACTGCCAGG AACCGATGGT ATGTGTCCAG
    AATCAGTGCC TCTGCCCGGA GAACAAGGTG TGCAACCAGT GCGCCACACA ACCGTGCCAG
    AATGGCGGTG AATGTGTGGA CCTACCGAAT GGAGACTACG AGTGCAAGTG CACCCGTGGA
    TGGACTGGAC GCACCTGTGG CAACGACGTG GACGAGTGCA CCCTGCATCC GAAGATCTGC
    GGCAATGGTA TCTGCAAGAA CGAGAAGGGA TCGTACAAAT GCTACTGTAC GCCTGGATTT
    ACCGGAGTCC ACTGCGATTC CGATGTGGAC GAATGCCTTA GCTTTCCCTG CCTCAACGGA
    GCCACGTGCC ACAACAAGTT GTTTTTCTTT AAATTCGGAA AGCATCGGAT TTTGAGACAA
    ATAAATGCCT ACGAGTGCGT TTGCCAACCA GGATATGAGG GAGAGAACTG CGAAGTAGAT
    ATCGATGAGT GCGGCAGTAA TCCCTGCTCG AACGGATCCA CCTGCATCGA CAGGATCAAC
    AACTTCACCT GCAACTGCAT TCCGGGAATG ACGGGCCGCA TATGTGACAT CGACATTGAC
    GACTGTGTGG GAGATCCCTG CTTGAATGGT GGTCAGTGCA TCGACCAATT GGGTGGCTTC
    CGTTGTGACT GCAGTGGCAC CGGTTATGAG GGCGAGAATT GTGAGCTGAA CATAGACGAA
    TGCCTCTCGA ATCCGTGCAC GAATGGTGCC AAGTGCCTGG ACAGGGTTAA GGACTACTTC
    TGCGATTGCC ACAACGGCTA CAAGGGAAAG AACTGTGAGC AGGACATCAA CGAGTGCGAG
    AGTAATCCCT GCCAGTACAA TGGCAACTGC CTAGAGCGTT CGAACATAAC GCTTTACCAA
    ATGAGTAGGA TCACGGATCT GCCCAAGGTG TTTAGCCAGC CCTTTAGCTT TGAGAATGCC
    AGCGGCTACG AGTGCGTCTG TGTGCCCGGC ATCATTGGAA AGAACTGCGA GATCAACATT
    AATGAGTGCG ACAGTAATCC ATGCAGCAAG CACGGAAATT GCAATGACGG GATTGGAACC
    TATACCTGCG AATGCGAACC TGGCTTCGAG GGCACCCACT GTGAGATCAA CATAGATGAA
    TGTGATCGCT ATAATCCCTG CCAGAGGGGC ACTTGCTATG ACCAGATAGA CGACTACGAC
    TGCGACTGCG ATGCGAACTA TGGAGGCAAA AACTGTTCCG TTCTTCTCAA AGGCTGTGAC
    CAGAATCCTT GTTTAAATGG CGGTGCCTGC TTGCCGTATT TGATCAACGA GGTAACTCAT
    CTGTACAACT GCACCTGCGA GAACGGCTTC CAGGGCGATA AATGCGAGAA GACCACCACT
    CTGTCCATGG TGGCCACTAG CCTGATTTCG GTTACCACGG AACGCGAGGA AGGCTACGAC
    ATTAATCTTC AATTTAGGAC CACACTGCCC AACGGAGTTT TGGCTTTCGG AACCACCGGC
    GAAAAGAATG AACCAGTTAG CTATATTTTG GAGCTGATTA ATGGACGACT GAATCTACAT
    TCCTCGCTCC TAAATAAGTG GGAGGGCGTG TTTATTGGAT CGAAGCTGAA CGATAGCAAC
    TGGCACAAGG TGTTTGTGGC CATAAACACT TCGCATCTAG TCCTTTCGGC CAACGACGAG
    CAGGCTATTT TTCCGGTTGG CTCCTATGAA ACGGCCAATA ATAGCCAGCC TTCATTCCCG
    CGCACATACC TCGGAGGCAC CATTCCCAAT CTGAAGTCCT ATCTGCGCCA CCTCACCCAC
    CAACCATCAG CTTTTGTGGG CTGCATGCAA GATATAATGG TCAATGGTAA ATGGATCTTT
    CCCGATGAGC AGGATGCGAA TATCAGTTAT ACCAAACTGG AAAATGTCCA GAGCGGTTGT
    CCGCGCACCG AACAATGTAA ACCGAATCCT TGCCATTCGA ACGGTGAATG CACGGACCTT
    TGGCACACCT TCGCTTGTCA CTGTCCCAGA CCATTCTTTG GGCACACATG TCAGCACAAT
    ATGACTGCTG CCACTTTTGG CCACGAGAAC ACAACCCATT CGGCCGTGAT TGTGGAGACC
    ACAGATGTGG CTAGGCGAGC TATTAGATCC ATCCTAGACA TTTCCATGTT TATTCGTACA
    CGCGAGCCAA CTGGTCAAGT CTTTTACTTG GGCACTGATC CACGCAAAGC CCCCACTAAA
    AATATTGGCG ACTCATATGT GGCCGCCAAA TTACACGGCG GCGAGCTGCT GGTAAAGATG
    CAGTTCAGCG GTACTCCGGA GGCCTACACC GTTGGTGGCC AGAAATTGGA CAACGGCTAT
    AACCACCTGA TCGAGGTGGT GCGCAATCAA ACGCTCGTGC AGGTCAAGCT CAATGGCACC
    GAGTACTTCC GCAAGACGCT GTCCACGACG GGTCTGCTGG ACGCACAGGT GCTTTACTTG
    GGCGGACCTG CACCCACACG CGAGTCACTT CTGGGCGCGA CCACAGAACC AGGAATAATT
    CCAGTTCCGG GAGCAGGAAT ACCCATTGAA GACACAACAG TGCCCAAGGA GGCGGACGAC
    AGCAGGGACT ACTTTAAGGG CATAATCCAG GACGTGAAGG TTAGCAATGG CTCGCTCAAC
    CTGATTGTGG AGATGTATTC CCTAAACGTG ACAGATGTCC AAGTGAATGC CAAACCTCTG
    GGCGCTGTAA CCATCGATCG TGCCTCCGTG TTACCTGGTG AGGTGTCCGA TGATCTGTGC
    CGAAAGAATC CCTGCCTCCA TAATGCCGAG TGCAGGAACA CATGGAATGA CTACACTTGC
    AAATGCCCAA ATGGTTACAA GGGAAAGAAC TGCCAGGAGA TCGAATTTTG CCAACACGTT
    ACATGTCCGG GACAGAGCTT GTGCCAAAAT CTGGACGATG GCTATGAGTG TGTTACGAAC
    ACCACATTTA CTGGGCAGGA ACGCAGTCCT CTCGCCTTCT TCTACTTCCA GGAGCAGCAA
    TCTGACGATA TTGTCAGTGA AGCGTCTCCC AAGCAAACTC TTAAACCGGT AATTGATATT
    GCCTTCCGCA CTCGTGCTGG AGGTACTCTC CTGTACATAG ACAATGTGGA CGGATTCTTT
    GAAATTGGAG TAAACGGAGG ACGAGTGACC ATCACCTGGA AGCTAAGTGC ACTGCATTTT
    GGCGAGTCTG CACGCTTTGA GAAGGAGAAC ACTGACGGAG AATGGAGTCG CATTTACCTC
    AGGGCACACA ACAGCAAACT GGAGGGTGGA TGGAAGGGAT GGGAATCGAT GGTGGATCCG
    ACGCCAGCTT TTTCCACGGA CATCGACCAA GCGGCCTTTC AGTCCCTGAT CGCTACAAGT
    ACCCAGGTTT ACTTGGGTGG CATGCCAGAG TCGCGACAAG CACGAGGATC CACTTTGTCT
    GCCCAGCAGG GCTCTCAGTT CAAGGGCTGT GTGGGAGAGG CAAGGGTGGG CGATCTTTTA
    CTGCCCTACT TCTCCATGGC GGAACTGTAT TCGCGCACCA ATGTTTCAGT ACAGCAAAAG
    GCTCAATTCC GTCTAAATGC CACACGACCT GAGGAGGGCT GCATCCTGTG CTTCCAGTCA
    GACTGCAAAA ATGACGGCTT CTGTCAATCT CCTTCAGATG AGTACGCCTG CACCTGTCAG
    CCTGGATTCG AGGGCGATGA TTGCGGCACG GACATCGACG AGTGTCTTAA CACGGAATGC
    TTGAACAACG GCACCTGCAT CAACCAGGTT GCAGCTTTCT TCTGCCAGTG TCAGCCAGGA
    TTCGAGGGTC AGCACTGTGA GCAAAACATC GACGAGTGTG CGGATCAGCC GTGCCACAAC
    GGTGGCAACT GCACGGATCT TATCGCATCG TACGTGTGCG ACTGCCCTGA GGACTATATG
    GGCCCGCAGT GCGACGTGCT GAAGCAAATG ACCTGCGAGA ACGAGCCATG TCGGAATGGA
    TCAACCTGCC AGAATGGATT CAATGCTTCT ACTGGCAATA ACTTTACATG TACATGCGTG
    CCCGGCTTCG AGGGTCCACT GTGTGACATA CCCTTCTGTG AAATAACGCC TTGCGATAAC
    GGTGGCCTCT GCCTGACCAC TGGAGCGGTA CCGATGTGCA AATGTAGTCT GGGATACACT
    GGTCGCCTGT GCGAGCAGGA CATTAACGAG TGCGAATCGA ATCCATGCCA GAACGGTGGT
    CAGTGTAAGG ACCTCGTCGG CAGGTACGAG TGCGATTGCC AGGGCACCGG GTTCGAGGGC
    ATTCGCTGTG AAAATGACAT CGACGAGTGC AACATGGAGG GAGATTACTG CGGCGGATTG
    GGCCGGTGTT TCAACAAGCC CGGATCCTTC CAGTGCATCT GCCAGAAACC CTATTGCGGA
    GCCTACTGCA ACTTTACGGA TCCCTGCAAC GCTACGGACC TCTGCTCAAA CGGCGGTCGC
    TGCGTAGAGT CCTGCGGCGC CAAACCGGAC TACTACTGCG AGTGTCCGGA AGGTTTCGCG
    GGAAAGAATT GCACAGCACC GATTACGGCC AAGGAGGACG GGCCTTCGAC CACAGACATT
    GCCATCATTG TAATACCCGT AGTGGTGGTG CTGCTGCTGA TCGCGGGAGC CCTCCTGGGC
    ACCTTCCTGG TGATGGCCAG GAACAAGCGA GCAACCAGGG GCACCTATAG CCCGAGCGCG
    CAAGAGTACT GCAACCCACG GCTGGAAATG GACAACGTAC TGAAGCCACC GCCGGAAGAG
    CGACTAATTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.