crb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	crb
Entrez Gene ID	42896
Full Name	crumbs
Synonyms	0509/20,1384/04,CG6383,CRB,CT19912,Crb,Crbs,Crumbs,DmCrb,Dmel\CG6383,crumb,far,l(3)07207,l(3)S050920,l(3)S058104,l(3)j1B5
General protein information	Preferred Names crumbs Names CG6383 gene product from transcript CG6383-RC CG6383-PA CG6383-PB CG6383-PC CG6383-PD crb-PA crb-PB crb-PC crb-PD fat rhabdomere fat rhabdomeres lethal (3) 07207
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 95F10-95F11\|3-82 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001260355.2	NP_001247284.1	crumbs, isoform C
NM_001043286.2	NP_001036751.1	crumbs, isoform B
NM_001275980.1	NP_001262909.1	crumbs, isoform D
NM_079756.3	NP_524480.2	crumbs, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	crb	NP_001247284.1
Caenorhabditis elegans (roundworm)	crb-1	NP_510822.1
Xenopus tropicalis (tropical clawed frog)	LOC100487355	XP_004918981.1
Gallus gallus (chicken)	CRB2	XP_004946067.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003873	XP_310433.5
Danio rerio (zebrafish)	crb2b	NP_001038627.1
Danio rerio (zebrafish)	crb2a	NP_001038764.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we find that EGFR regulates the apical determinant Crb and the extracellular matrix regulator Serp, two factors previously known to control tube length. EGFR regulates the organisation of endosomes in which Crb and Serp proteins are loaded
Title: EGFR controls Drosophila tracheal tube elongation by intracellular trafficking regulation.

The regulation of Crumbs by Neur is not strictly essential, it contributes to epithelium remodeling in the posterior midgut and thereby facilitates the trans-epithelial migration of the primordial germ cells in early embryos.
Title: Neuralized regulates Crumbs endocytosis and epithelium morphogenesis via specific Stardust isoforms.

The data provide a molecular basis to explain how the apical determinant Crb can regulate homeostasis of epithelial cells by stabilising Notch at the apical membrane, thereby finetuning Notch pathway activity.
Title: Drosophila Crumbs prevents ectopic Notch activation in developing wings by inhibiting ligand-independent endocytosis.

Study proposes that the interplay of Crb expression in the DV boundary and ubiquitin-dependent Crb downregulation by Ebi provides a mechanism for the maintenance of Notch signaling during wing development.
Title: Ebi modulates wing growth by ubiquitin-dependent downregulation of Crumbs in Drosophila.

The Crumbs:Par-6 crystal structure reveals specific PDZ-peptide contacts that contribute to its higher affinity.
Title: Binding of Crumbs to the Par-6 CRIB-PDZ Module Is Regulated by Cdc42.

The following crb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05934	NM_001043286.2 Latest version!	Drosophila melanogaster crumbs (crb), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa05935	NM_079756.3 Latest version!	Drosophila melanogaster crumbs (crb), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa08721	NM_001260355.2 Latest version!	Drosophila melanogaster crumbs (crb), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa08722	NM_001275980.1 Latest version!	Drosophila melanogaster crumbs (crb), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crb ( NM_001043286.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036751.1 Drosophila melanogaster crumbs (crb), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa05934
Clone ID Related Accession (Same CDS sequence)	NM_001043286.2
Accession Version	NM_001043286.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	crumbs, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_001043286.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTAAAA TCGCCAATGC GTCACTGTCG CAACAACAAA AACAGCGGCA AGCAGAAACA 
GCAACGACCA CAACAACAAC AGTGGCGGCA TCAGTAGAAA CAGCAACAAC AACAGCAAGA 
AGTCGAGACC GCACGAAAAG CGCAGCTCAA ATTACGTCGC ATTTGCTCAA GCGCGCCATT 
TCAGTTTATT CGTCGCCTCA ATGGATACCG CTCTTCATAC TTATTTACTT AGCAACAGAT 
GTCGCCTCAG TGGCGGTGCC GACGAAGGAG GCGTACTTTA ATGGCTCCAC TTACCTCCGC 
CTCACCACGC CGATGCCCAT TTGGGATCAC TCGGCGATTA GTTTCCGCTC GTGCCGCGGC 
GGCGAGATCC TCGCCCAGCA GTACAACAAG AACTCCATTG TAATCTCAGT GCTCAATGAC 
TTTCTGCAAA TCTCACTGGC TGGACCCGCC GTCCATGGGC CCAACAACCG GCTGGATGTC 
AAGCTGCCCT ACCAACTGCT GGACAACCGC TGGCATACGC TGCAGTTCAA GTACGAGTAC 
GGAAATCTCT ACCTGCATGT GGATCGCGCG GCAAGCATAT TTGCCAACTC CACGTACAAC 
AGTCAGTTCC TGACTAACCA GGACATTGGC TACAAGGACG CCATCTTGAT ACTGGGAAAC 
TCCTTCTCCG GTTGCCTTCT GGATGGACCA GGTCTGCAAT TTGTGAACAA CTCGACGGTG 
CAGAATGTGG TCTTTGGTCA CTGTCCCTTG ACCCCGGGTC CCTGCAGCGA TCACGATCTT 
TTCACTCGAC TGCCAGACAA CTTTTGTCTG AATGATCCCT GTATGGGCCA TGGAACCTGC 
TCGTCCAGTC CAGAGGGATA CGAATGTCGG TGCACGGCAC GTTACTCGGG AAAAAATTGC 
CAGAAGGACA ATGGCTCGCC GTGTGCCAAA AATCCATGTG AGAACGGTGG TTCCTGCCTG 
GAGAATTCCC GAGGGGATTA CCAGTGCTTC TGTGATCCCA ACCACAGTGG TCAGCACTGC 
GAGACGGAGG TGAACATCCA CCCACTCTGC CAGACGAACC CCTGCCTGAA CAATGGAGCA 
TGTGTGGTGA TTGGCGGCAG TGGAGCACTC ACCTGCGAGT GTCCCAAAGG ATACGCTGGT 
GCCAGGTGCG AGGTGGACAC GGATGAGTGC GCCTCACAGC CGTGTCAGAA CAACGGAAGC 
TGCATAGACC GTATCAATGG ATTCAGCTGC GATTGCAGCG GCACCGGTTA CACGGGTGCG 
TTCTGCCAGA CGAATGTGGA TGAGTGCGAC AAGAATCCGT GCCTGAACGG CGGCAGATGC 
TTCGATACCT ACGGATGGTA CACCTGCCAG TGTCTGGATG GTTGGGGCGG CGAGATTTGT 
GATCGACCCA TGACATGCCA GACGCAGCAG TGTTTGAACG GTGGCACCTG CCTGGACAAG 
CCCATCGGCT TCCAGTGCCT CTGTCCGCCG GAGTACACTG GTGAACTGTG CCAAATCGCA 
CCCAGTTGCG CACAGCAGTG CCCCATCGAT TCGGAGTGCG TAGGCGGCAA ATGCGTTTGT 
AAGCCAGGCT CATCGGCTTC CGACATGGAG CCGCTGACTC CGCTCGAGCT GGACATTCTC 
GACGCCACGC TGTGCCCCAG CGAAAAGAAG AAGCGCTACA TATCGCCCGA GTGGCTCAAA 
CGCAAGCGTT GTGAGCTAAA ACTAAGTTAC AACTGTCAAA CGAGCACGGG CGATGGAGCT 
TCTGCTTTGG CCCTGACACC CATCAACTGC AATGCCACCA ATGGAAAGTG TCTCAATGGA 
GGAACTTGCT CCATGAACGG AACCCACTGC TATTGCGCCG TCGGCTATTC GGGAGATCGT 
TGCGAGAAGG CCGAGAACTG TTCGCCACTG AACTGCCAGG AACCGATGGT ATGTGTCCAG 
AATCAGTGCC TCTGCCCGGA GAACAAGGTG TGCAACCAGT GCGCCACACA ACCGTGCCAG 
AATGGCGGTG AATGTGTGGA CCTACCGAAT GGAGACTACG AGTGCAAGTG CACCCGTGGA 
TGGACTGGAC GCACCTGTGG CAACGACGTG GACGAGTGCA CCCTGCATCC GAAGATCTGC 
GGCAATGGTA TCTGCAAGAA CGAGAAGGGA TCGTACAAAT GCTACTGTAC GCCTGGATTT 
ACCGGAGTCC ACTGCGATTC CGATGTGGAC GAATGCCTTA GCTTTCCCTG CCTCAACGGA 
GCCACGTGCC ACAACAAGAT AAATGCCTAC GAGTGCGTTT GCCAACCAGG ATATGAGGGA 
GAGAACTGCG AAGTAGATAT CGATGAGTGC GGCAGTAATC CCTGCTCGAA CGGATCCACC 
TGCATCGACA GGATCAACAA CTTCACCTGC AACTGCATTC CGGGAATGAC GGGCCGCATA 
TGTGACATCG ACATTGACGA CTGTGTGGGA GATCCCTGCT TGAATGGTGG TCAGTGCATC 
GACCAATTGG GTGGCTTCCG TTGTGACTGC AGTGGCACCG GTTATGAGGG CGAGAATTGT 
GAGCTGAACA TAGACGAATG CCTCTCGAAT CCGTGCACGA ATGGTGCCAA GTGCCTGGAC 
AGGGTTAAGG ACTACTTCTG CGATTGCCAC AACGGCTACA AGGGAAAGAA CTGTGAGCAG 
GACATCAACG AGTGCGAGAG TAATCCCTGC CAGTACAATG GCAACTGCCT AGAGCGTTCG 
AACATAACGC TTTACCAAAT GAGTAGGATC ACGGATCTGC CCAAGGTGTT TAGCCAGCCC 
TTTAGCTTTG AGAATGCCAG CGGCTACGAG TGCGTCTGTG TGCCCGGCAT CATTGGAAAG 
AACTGCGAGA TCAACATTAA TGAGTGCGAC AGTAATCCAT GCAGCAAGCA CGGAAATTGC 
AATGACGGGA TTGGAACCTA TACCTGCGAA TGCGAACCTG GCTTCGAGGG CACCCACTGT 
GAGATCAACA TAGATGAATG TGATCGCTAT AATCCCTGCC AGAGGGGCAC TTGCTATGAC 
CAGATAGACG ACTACGACTG CGACTGCGAT GCGAACTATG GAGGCAAAAA CTGTTCCGTT 
CTTCTCAAAG GCTGTGACCA GAATCCTTGT TTAAATGGCG GTGCCTGCTT GCCGTATTTG 
ATCAACGAGG TAACTCATCT GTACAACTGC ACCTGCGAGA ACGGCTTCCA GGGCGATAAA 
TGCGAGAAGA CCACCACTCT GTCCATGGTG GCCACTAGCC TGATTTCGGT TACCACGGAA 
CGCGAGGAAG GCTACGACAT TAATCTTCAA TTTAGGACCA CACTGCCCAA CGGAGTTTTG 
GCTTTCGGAA CCACCGGCGA AAAGAATGAA CCAGTTAGCT ATATTTTGGA GCTGATTAAT 
GGACGACTGA ATCTACATTC CTCGCTCCTA AATAAGTGGG AGGGCGTGTT TATTGGATCG 
AAGCTGAACG ATAGCAACTG GCACAAGGTG TTTGTGGCCA TAAACACTTC GCATCTAGTC 
CTTTCGGCCA ACGACGAGCA GGCTATTTTT CCGGTTGGCT CCTATGAAAC GGCCAATAAT 
AGCCAGCCTT CATTCCCGCG CACATACCTC GGAGGCACCA TTCCCAATCT GAAGTCCTAT 
CTGCGCCACC TCACCCACCA ACCATCAGCT TTTGTGGGCT GCATGCAAGA TATAATGGTC 
AATGGTAAAT GGATCTTTCC CGATGAGCAG GATGCGAATA TCAGTTATAC CAAACTGGAA 
AATGTCCAGA GCGGTTGTCC GCGCACCGAA CAATGTAAAC CGAATCCTTG CCATTCGAAC 
GGTGAATGCA CGGACCTTTG GCACACCTTC GCTTGTCACT GTCCCAGACC ATTCTTTGGG 
CACACATGTC AGCACAATAT GACTGCTGCC ACTTTTGGCC ACGAGAACAC AACCCATTCG 
GCCGTGATTG TGGAGACCAC AGATGTGGCT AGGCGAGCTA TTAGATCCAT CCTAGACATT 
TCCATGTTTA TTCGTACACG CGAGCCAACT GGTCAAGTCT TTTACTTGGG CACTGATCCA 
CGCAAAGCCC CCACTAAAAA TATTGGCGAC TCATATGTGG CCGCCAAATT ACACGGCGGC 
GAGCTGCTGG TAAAGATGCA GTTCAGCGGT ACTCCGGAGG CCTACACCGT TGGTGGCCAG 
AAATTGGACA ACGGCTATAA CCACCTGATC GAGGTGGTGC GCAATCAAAC GCTCGTGCAG 
GTCAAGCTCA ATGGCACCGA GTACTTCCGC AAGACGCTGT CCACGACGGG TCTGCTGGAC 
GCACAGGTGC TTTACTTGGG CGGACCTGCA CCCACACGCG AGTCACTTCT GGGCGCGACC 
ACAGAACCAG GAATAATTCC AGTTCCGGGA GCAGGAATAC CCATTGAAGA CACAACAGTG 
CCCAAGGAGG CGGACGACAG CAGGGACTAC TTTAAGGGCA TAATCCAGGA CGTGAAGGTT 
AGCAATGGCT CGCTCAACCT GATTGTGGAG ATGTATTCCC TAAACGTGAC AGATGTCCAA 
GTGAATGCCA AACCTCTGGG CGCTGTAACC ATCGATCGTG CCTCCGTGTT ACCTGGTGAG 
GTGTCCGATG ATCTGTGCCG AAAGAATCCC TGCCTCCATA ATGCCGAGTG CAGGAACACA 
TGGAATGACT ACACTTGCAA ATGCCCAAAT GGTTACAAGG GAAAGAACTG CCAGGAGATC 
GAATTTTGCC AACACGTTAC ATGTCCGGGA CAGAGCTTGT GCCAAAATCT GGACGATGGC 
TATGAGTGTG TTACGAACAC CACATTTACT GGGCAGGAAC GCAGTCCTCT CGCCTTCTTC 
TACTTCCAGG AGCAGCAATC TGACGATATT GTCAGTGAAG CGTCTCCCAA GCAAACTCTT 
AAACCGGTAA TTGATATTGC CTTCCGCACT CGTGCTGGAG GTACTCTCCT GTACATAGAC 
AATGTGGACG GATTCTTTGA AATTGGAGTA AACGGAGGAC GAGTGACCAT CACCTGGAAG 
CTAAGTGCAC TGCATTTTGG CGAGTCTGCA CGCTTTGAGA AGGAGAACAC TGACGGAGAA 
TGGAGTCGCA TTTACCTCAG GGCACACAAC AGCAAACTGG AGGGTGGATG GAAGGGATGG 
GAATCGATGG TGGATCCGAC GCCAGCTTTT TCCACGGACA TCGACCAAGC GGCCTTTCAG 
TCCCTGATCG CTACAAGTAC CCAGGTTTAC TTGGGTGGCA TGCCAGAGTC GCGACAAGCA 
CGAGGATCCA CTTTGTCTGC CCAGCAGGGC TCTCAGTTCA AGGGCTGTGT GGGAGAGGCA 
AGGGTGGGCG ATCTTTTACT GCCCTACTTC TCCATGGCGG AACTGTATTC GCGCACCAAT 
GTTTCAGTAC AGCAAAAGGC TCAATTCCGT CTAAATGCCA CACGACCTGA GGAGGGCTGC 
ATCCTGTGCT TCCAGTCAGA CTGCAAAAAT GACGGCTTCT GTCAATCTCC TTCAGATGAG 
TACGCCTGCA CCTGTCAGCC TGGATTCGAG GGCGATGATT GCGGCACGGA CATCGACGAG 
TGTCTTAACA CGGAATGCTT GAACAACGGC ACCTGCATCA ACCAGGTTGC AGCTTTCTTC 
TGCCAGTGTC AGCCAGGATT CGAGGGTCAG CACTGTGAGC AAAACATCGA CGAGTGTGCG 
GATCAGCCGT GCCACAACGG TGGCAACTGC ACGGATCTTA TCGCATCGTA CGTGTGCGAC 
TGCCCTGAGG ACTATATGGG CCCGCAGTGC GACGTGCTGA AGCAAATGAC CTGCGAGAAC 
GAGCCATGTC GGAATGGATC AACCTGCCAG AATGGATTCA ATGCTTCTAC TGGCAATAAC 
TTTACATGTA CATGCGTGCC CGGCTTCGAG GGTCCACTGT GTGACATACC CTTCTGTGAA 
ATAACGCCTT GCGATAACGG TGGCCTCTGC CTGACCACTG GAGCGGTACC GATGTGCAAA 
TGTAGTCTGG GATACACTGG TCGCCTGTGC GAGCAGGACA TTAACGAGTG CGAATCGAAT 
CCATGCCAGA ACGGTGGTCA GTGTAAGGAC CTCGTCGGCA GGTACGAGTG CGATTGCCAG 
GGCACCGGGT TCGAGGGCAT TCGCTGTGAA AATGACATCG ACGAGTGCAA CATGGAGGGA 
GATTACTGCG GCGGATTGGG CCGGTGTTTC AACAAGCCCG GATCCTTCCA GTGCATCTGC 
CAGAAACCCT ATTGCGGAGC CTACTGCAAC TTTACGGATC CCTGCAACGC TACGGACCTC 
TGCTCAAACG GCGGTCGCTG CGTAGAGTCC TGCGGCGCCA AACCGGACTA CTACTGCGAG 
TGTCCGGAAG GTTTCGCGGG AAAGAATTGC ACAGCACCGA TTACGGCCAA GGAGGACGGG 
CCTTCGACCA CAGACATTGC CATCATTGTA ATACCCGTAG TGGTGGTGCT GCTGCTGATC 
GCGGGAGCCC TCCTGGGCAC CTTCCTGGTG ATGGCCAGGA ACAAGCGAGC AACCAGGGGC 
ACCTATAGCC CGAGCGCGCA AGAGTACTGC AACCCACGGC TGGAAATGGA CAACGTACTG 
AAGCCACCGC CGGAAGAGCG ACTAATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036751.1
CDS	550..7119
Translation	MAKIANASLSQQQKQRQAETATTTTTTVAASVETATTTARSRDRTKSAAQITSHLLKRAISVYSSPQWIPLFILIYLATDVASVAVPTKEAYFNGSTYLRLTTPMPIWDHSAISFRSCRGGEILAQQYNKNSIVISVLNDFLQISLAGPAVHGPNNRLDVKLPYQLLDNRWHTLQFKYEYGNLYLHVDRAASIFANSTYNSQFLTNQDIGYKDAILILGNSFSGCLLDGPGLQFVNNSTVQNVVFGHCPLTPGPCSDHDLFTRLPDNFCLNDPCMGHGTCSSSPEGYECRCTARYSGKNCQKDNGSPCAKNPCENGGSCLENSRGDYQCFCDPNHSGQHCETEVNIHPLCQTNPCLNNGACVVIGGSGALTCECPKGYAGARCEVDTDECASQPCQNNGSCIDRINGFSCDCSGTGYTGAFCQTNVDECDKNPCLNGGRCFDTYGWYTCQCLDGWGGEICDRPMTCQTQQCLNGGTCLDKPIGFQCLCPPEYTGELCQIAPSCAQQCPIDSECVGGKCVCKPGSSASDMEPLTPLELDILDATLCPSEKKKRYISPEWLKRKRCELKLSYNCQTSTGDGASALALTPINCNATNGKCLNGGTCSMNGTHCYCAVGYSGDRCEKAENCSPLNCQEPMVCVQNQCLCPENKVCNQCATQPCQNGGECVDLPNGDYECKCTRGWTGRTCGNDVDECTLHPKICGNGICKNEKGSYKCYCTPGFTGVHCDSDVDECLSFPCLNGATCHNKINAYECVCQPGYEGENCEVDIDECGSNPCSNGSTCIDRINNFTCNCIPGMTGRICDIDIDDCVGDPCLNGGQCIDQLGGFRCDCSGTGYEGENCELNIDECLSNPCTNGAKCLDRVKDYFCDCHNGYKGKNCEQDINECESNPCQYNGNCLERSNITLYQMSRITDLPKVFSQPFSFENASGYECVCVPGIIGKNCEININECDSNPCSKHGNCNDGIGTYTCECEPGFEGTHCEINIDECDRYNPCQRGTCYDQIDDYDCDCDANYGGKNCSVLLKGCDQNPCLNGGACLPYLINEVTHLYNCTCENGFQGDKCEKTTTLSMVATSLISVTTEREEGYDINLQFRTTLPNGVLAFGTTGEKNEPVSYILELINGRLNLHSSLLNKWEGVFIGSKLNDSNWHKVFVAINTSHLVLSANDEQAIFPVGSYETANNSQPSFPRTYLGGTIPNLKSYLRHLTHQPSAFVGCMQDIMVNGKWIFPDEQDANISYTKLENVQSGCPRTEQCKPNPCHSNGECTDLWHTFACHCPRPFFGHTCQHNMTAATFGHENTTHSAVIVETTDVARRAIRSILDISMFIRTREPTGQVFYLGTDPRKAPTKNIGDSYVAAKLHGGELLVKMQFSGTPEAYTVGGQKLDNGYNHLIEVVRNQTLVQVKLNGTEYFRKTLSTTGLLDAQVLYLGGPAPTRESLLGATTEPGIIPVPGAGIPIEDTTVPKEADDSRDYFKGIIQDVKVSNGSLNLIVEMYSLNVTDVQVNAKPLGAVTIDRASVLPGEVSDDLCRKNPCLHNAECRNTWNDYTCKCPNGYKGKNCQEIEFCQHVTCPGQSLCQNLDDGYECVTNTTFTGQERSPLAFFYFQEQQSDDIVSEASPKQTLKPVIDIAFRTRAGGTLLYIDNVDGFFEIGVNGGRVTITWKLSALHFGESARFEKENTDGEWSRIYLRAHNSKLEGGWKGWESMVDPTPAFSTDIDQAAFQSLIATSTQVYLGGMPESRQARGSTLSAQQGSQFKGCVGEARVGDLLLPYFSMAELYSRTNVSVQQKAQFRLNATRPEEGCILCFQSDCKNDGFCQSPSDEYACTCQPGFEGDDCGTDIDECLNTECLNNGTCINQVAAFFCQCQPGFEGQHCEQNIDECADQPCHNGGNCTDLIASYVCDCPEDYMGPQCDVLKQMTCENEPCRNGSTCQNGFNASTGNNFTCTCVPGFEGPLCDIPFCEITPCDNGGLCLTTGAVPMCKCSLGYTGRLCEQDINECESNPCQNGGQCKDLVGRYECDCQGTGFEGIRCENDIDECNMEGDYCGGLGRCFNKPGSFQCICQKPYCGAYCNFTDPCNATDLCSNGGRCVESCGAKPDYYCECPEGFAGKNCTAPITAKEDGPSTTDIAIIVIPVVVVLLLIAGALLGTFLVMARNKRATRGTYSPSAQEYCNPRLEMDNVLKPPPEERLI

Target ORF information:

RefSeq Version	NM_001043286.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster crumbs (crb), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043286.2

ORF Insert Sequence:

ATGGCTAAAA TCGCCAATGC GTCACTGTCG CAACAACAAA AACAGCGGCA AGCAGAAACA 
GCAACGACCA CAACAACAAC AGTGGCGGCA TCAGTAGAAA CAGCAACAAC AACAGCAAGA 
AGTCGAGACC GCACGAAAAG CGCAGCTCAA ATTACGTCGC ATTTGCTCAA GCGCGCCATT 
TCAGTTTATT CGTCGCCTCA ATGGATACCG CTCTTCATAC TTATTTACTT AGCAACAGAT 
GTCGCCTCAG TGGCGGTGCC GACGAAGGAG GCGTACTTTA ATGGCTCCAC TTACCTCCGC 
CTCACCACGC CGATGCCCAT TTGGGATCAC TCGGCGATTA GTTTCCGCTC GTGCCGCGGC 
GGCGAGATCC TCGCCCAGCA GTACAACAAG AACTCCATTG TAATCTCAGT GCTCAATGAC 
TTTCTGCAAA TCTCACTGGC TGGACCCGCC GTCCATGGGC CCAACAACCG GCTGGATGTC 
AAGCTGCCCT ACCAACTGCT GGACAACCGC TGGCATACGC TGCAGTTCAA GTACGAGTAC 
GGAAATCTCT ACCTGCATGT GGATCGCGCG GCAAGCATAT TTGCCAACTC CACGTACAAC 
AGTCAGTTCC TGACTAACCA GGACATTGGC TACAAGGACG CCATCTTGAT ACTGGGAAAC 
TCCTTCTCCG GTTGCCTTCT GGATGGACCA GGTCTGCAAT TTGTGAACAA CTCGACGGTG 
CAGAATGTGG TCTTTGGTCA CTGTCCCTTG ACCCCGGGTC CCTGCAGCGA TCACGATCTT 
TTCACTCGAC TGCCAGACAA CTTTTGTCTG AATGATCCCT GTATGGGCCA TGGAACCTGC 
TCGTCCAGTC CAGAGGGATA CGAATGTCGG TGCACGGCAC GTTACTCGGG AAAAAATTGC 
CAGAAGGACA ATGGCTCGCC GTGTGCCAAA AATCCATGTG AGAACGGTGG TTCCTGCCTG 
GAGAATTCCC GAGGGGATTA CCAGTGCTTC TGTGATCCCA ACCACAGTGG TCAGCACTGC 
GAGACGGAGG TGAACATCCA CCCACTCTGC CAGACGAACC CCTGCCTGAA CAATGGAGCA 
TGTGTGGTGA TTGGCGGCAG TGGAGCACTC ACCTGCGAGT GTCCCAAAGG ATACGCTGGT 
GCCAGGTGCG AGGTGGACAC GGATGAGTGC GCCTCACAGC CGTGTCAGAA CAACGGAAGC 
TGCATAGACC GTATCAATGG ATTCAGCTGC GATTGCAGCG GCACCGGTTA CACGGGTGCG 
TTCTGCCAGA CGAATGTGGA TGAGTGCGAC AAGAATCCGT GCCTGAACGG CGGCAGATGC 
TTCGATACCT ACGGATGGTA CACCTGCCAG TGTCTGGATG GTTGGGGCGG CGAGATTTGT 
GATCGACCCA TGACATGCCA GACGCAGCAG TGTTTGAACG GTGGCACCTG CCTGGACAAG 
CCCATCGGCT TCCAGTGCCT CTGTCCGCCG GAGTACACTG GTGAACTGTG CCAAATCGCA 
CCCAGTTGCG CACAGCAGTG CCCCATCGAT TCGGAGTGCG TAGGCGGCAA ATGCGTTTGT 
AAGCCAGGCT CATCGGCTTC CGACATGGAG CCGCTGACTC CGCTCGAGCT GGACATTCTC 
GACGCCACGC TGTGCCCCAG CGAAAAGAAG AAGCGCTACA TATCGCCCGA GTGGCTCAAA 
CGCAAGCGTT GTGAGCTAAA ACTAAGTTAC AACTGTCAAA CGAGCACGGG CGATGGAGCT 
TCTGCTTTGG CCCTGACACC CATCAACTGC AATGCCACCA ATGGAAAGTG TCTCAATGGA 
GGAACTTGCT CCATGAACGG AACCCACTGC TATTGCGCCG TCGGCTATTC GGGAGATCGT 
TGCGAGAAGG CCGAGAACTG TTCGCCACTG AACTGCCAGG AACCGATGGT ATGTGTCCAG 
AATCAGTGCC TCTGCCCGGA GAACAAGGTG TGCAACCAGT GCGCCACACA ACCGTGCCAG 
AATGGCGGTG AATGTGTGGA CCTACCGAAT GGAGACTACG AGTGCAAGTG CACCCGTGGA 
TGGACTGGAC GCACCTGTGG CAACGACGTG GACGAGTGCA CCCTGCATCC GAAGATCTGC 
GGCAATGGTA TCTGCAAGAA CGAGAAGGGA TCGTACAAAT GCTACTGTAC GCCTGGATTT 
ACCGGAGTCC ACTGCGATTC CGATGTGGAC GAATGCCTTA GCTTTCCCTG CCTCAACGGA 
GCCACGTGCC ACAACAAGAT AAATGCCTAC GAGTGCGTTT GCCAACCAGG ATATGAGGGA 
GAGAACTGCG AAGTAGATAT CGATGAGTGC GGCAGTAATC CCTGCTCGAA CGGATCCACC 
TGCATCGACA GGATCAACAA CTTCACCTGC AACTGCATTC CGGGAATGAC GGGCCGCATA 
TGTGACATCG ACATTGACGA CTGTGTGGGA GATCCCTGCT TGAATGGTGG TCAGTGCATC 
GACCAATTGG GTGGCTTCCG TTGTGACTGC AGTGGCACCG GTTATGAGGG CGAGAATTGT 
GAGCTGAACA TAGACGAATG CCTCTCGAAT CCGTGCACGA ATGGTGCCAA GTGCCTGGAC 
AGGGTTAAGG ACTACTTCTG CGATTGCCAC AACGGCTACA AGGGAAAGAA CTGTGAGCAG 
GACATCAACG AGTGCGAGAG TAATCCCTGC CAGTACAATG GCAACTGCCT AGAGCGTTCG 
AACATAACGC TTTACCAAAT GAGTAGGATC ACGGATCTGC CCAAGGTGTT TAGCCAGCCC 
TTTAGCTTTG AGAATGCCAG CGGCTACGAG TGCGTCTGTG TGCCCGGCAT CATTGGAAAG 
AACTGCGAGA TCAACATTAA TGAGTGCGAC AGTAATCCAT GCAGCAAGCA CGGAAATTGC 
AATGACGGGA TTGGAACCTA TACCTGCGAA TGCGAACCTG GCTTCGAGGG CACCCACTGT 
GAGATCAACA TAGATGAATG TGATCGCTAT AATCCCTGCC AGAGGGGCAC TTGCTATGAC 
CAGATAGACG ACTACGACTG CGACTGCGAT GCGAACTATG GAGGCAAAAA CTGTTCCGTT 
CTTCTCAAAG GCTGTGACCA GAATCCTTGT TTAAATGGCG GTGCCTGCTT GCCGTATTTG 
ATCAACGAGG TAACTCATCT GTACAACTGC ACCTGCGAGA ACGGCTTCCA GGGCGATAAA 
TGCGAGAAGA CCACCACTCT GTCCATGGTG GCCACTAGCC TGATTTCGGT TACCACGGAA 
CGCGAGGAAG GCTACGACAT TAATCTTCAA TTTAGGACCA CACTGCCCAA CGGAGTTTTG 
GCTTTCGGAA CCACCGGCGA AAAGAATGAA CCAGTTAGCT ATATTTTGGA GCTGATTAAT 
GGACGACTGA ATCTACATTC CTCGCTCCTA AATAAGTGGG AGGGCGTGTT TATTGGATCG 
AAGCTGAACG ATAGCAACTG GCACAAGGTG TTTGTGGCCA TAAACACTTC GCATCTAGTC 
CTTTCGGCCA ACGACGAGCA GGCTATTTTT CCGGTTGGCT CCTATGAAAC GGCCAATAAT 
AGCCAGCCTT CATTCCCGCG CACATACCTC GGAGGCACCA TTCCCAATCT GAAGTCCTAT 
CTGCGCCACC TCACCCACCA ACCATCAGCT TTTGTGGGCT GCATGCAAGA TATAATGGTC 
AATGGTAAAT GGATCTTTCC CGATGAGCAG GATGCGAATA TCAGTTATAC CAAACTGGAA 
AATGTCCAGA GCGGTTGTCC GCGCACCGAA CAATGTAAAC CGAATCCTTG CCATTCGAAC 
GGTGAATGCA CGGACCTTTG GCACACCTTC GCTTGTCACT GTCCCAGACC ATTCTTTGGG 
CACACATGTC AGCACAATAT GACTGCTGCC ACTTTTGGCC ACGAGAACAC AACCCATTCG 
GCCGTGATTG TGGAGACCAC AGATGTGGCT AGGCGAGCTA TTAGATCCAT CCTAGACATT 
TCCATGTTTA TTCGTACACG CGAGCCAACT GGTCAAGTCT TTTACTTGGG CACTGATCCA 
CGCAAAGCCC CCACTAAAAA TATTGGCGAC TCATATGTGG CCGCCAAATT ACACGGCGGC 
GAGCTGCTGG TAAAGATGCA GTTCAGCGGT ACTCCGGAGG CCTACACCGT TGGTGGCCAG 
AAATTGGACA ACGGCTATAA CCACCTGATC GAGGTGGTGC GCAATCAAAC GCTCGTGCAG 
GTCAAGCTCA ATGGCACCGA GTACTTCCGC AAGACGCTGT CCACGACGGG TCTGCTGGAC 
GCACAGGTGC TTTACTTGGG CGGACCTGCA CCCACACGCG AGTCACTTCT GGGCGCGACC 
ACAGAACCAG GAATAATTCC AGTTCCGGGA GCAGGAATAC CCATTGAAGA CACAACAGTG 
CCCAAGGAGG CGGACGACAG CAGGGACTAC TTTAAGGGCA TAATCCAGGA CGTGAAGGTT 
AGCAATGGCT CGCTCAACCT GATTGTGGAG ATGTATTCCC TAAACGTGAC AGATGTCCAA 
GTGAATGCCA AACCTCTGGG CGCTGTAACC ATCGATCGTG CCTCCGTGTT ACCTGGTGAG 
GTGTCCGATG ATCTGTGCCG AAAGAATCCC TGCCTCCATA ATGCCGAGTG CAGGAACACA 
TGGAATGACT ACACTTGCAA ATGCCCAAAT GGTTACAAGG GAAAGAACTG CCAGGAGATC 
GAATTTTGCC AACACGTTAC ATGTCCGGGA CAGAGCTTGT GCCAAAATCT GGACGATGGC 
TATGAGTGTG TTACGAACAC CACATTTACT GGGCAGGAAC GCAGTCCTCT CGCCTTCTTC 
TACTTCCAGG AGCAGCAATC TGACGATATT GTCAGTGAAG CGTCTCCCAA GCAAACTCTT 
AAACCGGTAA TTGATATTGC CTTCCGCACT CGTGCTGGAG GTACTCTCCT GTACATAGAC 
AATGTGGACG GATTCTTTGA AATTGGAGTA AACGGAGGAC GAGTGACCAT CACCTGGAAG 
CTAAGTGCAC TGCATTTTGG CGAGTCTGCA CGCTTTGAGA AGGAGAACAC TGACGGAGAA 
TGGAGTCGCA TTTACCTCAG GGCACACAAC AGCAAACTGG AGGGTGGATG GAAGGGATGG 
GAATCGATGG TGGATCCGAC GCCAGCTTTT TCCACGGACA TCGACCAAGC GGCCTTTCAG 
TCCCTGATCG CTACAAGTAC CCAGGTTTAC TTGGGTGGCA TGCCAGAGTC GCGACAAGCA 
CGAGGATCCA CTTTGTCTGC CCAGCAGGGC TCTCAGTTCA AGGGCTGTGT GGGAGAGGCA 
AGGGTGGGCG ATCTTTTACT GCCCTACTTC TCCATGGCGG AACTGTATTC GCGCACCAAT 
GTTTCAGTAC AGCAAAAGGC TCAATTCCGT CTAAATGCCA CACGACCTGA GGAGGGCTGC 
ATCCTGTGCT TCCAGTCAGA CTGCAAAAAT GACGGCTTCT GTCAATCTCC TTCAGATGAG 
TACGCCTGCA CCTGTCAGCC TGGATTCGAG GGCGATGATT GCGGCACGGA CATCGACGAG 
TGTCTTAACA CGGAATGCTT GAACAACGGC ACCTGCATCA ACCAGGTTGC AGCTTTCTTC 
TGCCAGTGTC AGCCAGGATT CGAGGGTCAG CACTGTGAGC AAAACATCGA CGAGTGTGCG 
GATCAGCCGT GCCACAACGG TGGCAACTGC ACGGATCTTA TCGCATCGTA CGTGTGCGAC 
TGCCCTGAGG ACTATATGGG CCCGCAGTGC GACGTGCTGA AGCAAATGAC CTGCGAGAAC 
GAGCCATGTC GGAATGGATC AACCTGCCAG AATGGATTCA ATGCTTCTAC TGGCAATAAC 
TTTACATGTA CATGCGTGCC CGGCTTCGAG GGTCCACTGT GTGACATACC CTTCTGTGAA 
ATAACGCCTT GCGATAACGG TGGCCTCTGC CTGACCACTG GAGCGGTACC GATGTGCAAA 
TGTAGTCTGG GATACACTGG TCGCCTGTGC GAGCAGGACA TTAACGAGTG CGAATCGAAT 
CCATGCCAGA ACGGTGGTCA GTGTAAGGAC CTCGTCGGCA GGTACGAGTG CGATTGCCAG 
GGCACCGGGT TCGAGGGCAT TCGCTGTGAA AATGACATCG ACGAGTGCAA CATGGAGGGA 
GATTACTGCG GCGGATTGGG CCGGTGTTTC AACAAGCCCG GATCCTTCCA GTGCATCTGC 
CAGAAACCCT ATTGCGGAGC CTACTGCAAC TTTACGGATC CCTGCAACGC TACGGACCTC 
TGCTCAAACG GCGGTCGCTG CGTAGAGTCC TGCGGCGCCA AACCGGACTA CTACTGCGAG 
TGTCCGGAAG GTTTCGCGGG AAAGAATTGC ACAGCACCGA TTACGGCCAA GGAGGACGGG 
CCTTCGACCA CAGACATTGC CATCATTGTA ATACCCGTAG TGGTGGTGCT GCTGCTGATC 
GCGGGAGCCC TCCTGGGCAC CTTCCTGGTG ATGGCCAGGA ACAAGCGAGC AACCAGGGGC 
ACCTATAGCC CGAGCGCGCA AGAGTACTGC AACCCACGGC TGGAAATGGA CAACGTACTG 
AAGCCACCGC CGGAAGAGCG ACTAATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crb ( NM_079756.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524480.2 Drosophila melanogaster crumbs (crb), transcript variant A, mRNA.

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CloneID	OFa05935
Clone ID Related Accession (Same CDS sequence)	NM_079756.3
Accession Version	NM_079756.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	crumbs, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_079756.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTAAAA TCGCCAATGC GTCACTGTCG CAACAACAAA AACAGCGGCA AGCAGAAACA 
GCAACGACCA CAACAACAAC AGTGGCGGCA TCAGTAGAAA CAGCAACAAC AACAGCAAGA 
AGTCGAGACC GCACGAAAAG CGCAGCTCAA ATTACGTCGC ATTTGCTCAA GCGCGCCATT 
TCAGTTTATT CGTCGCCTCA ATGGATACCG CTCTTCATAC TTATTTACTT AGCAACAGAT 
GTCGCCTCAG TGGCGGTGCC GACGAAGGAG GCGTACTTTA ATGGCTCCAC TTACCTCCGC 
CTCACCACGC CGATGCCCAT TTGGGATCAC TCGGCGATTA GTTTCCGCTC GTGCCGCGGC 
GGCGAGATCC TCGCCCAGCA GTACAACAAG AACTCCATTG TAATCTCAGT GCTCAATGAC 
TTTCTGCAAA TCTCACTGGC TGGACCCGCC GTCCATGGGC CCAACAACCG GCTGGATGTC 
AAGCTGCCCT ACCAACTGCT GGACAACCGC TGGCATACGC TGCAGTTCAA GTACGAGTAC 
GGAAATCTCT ACCTGCATGT GGATCGCGCG GCAAGCATAT TTGCCAACTC CACGTACAAC 
AGTCAGTTCC TGACTAACCA GGACATTGGC TACAAGGACG CCATCTTGAT ACTGGGAAAC 
TCCTTCTCCG GTTGCCTTCT GGATGGACCA GGTCTGCAAT TTGTGAACAA CTCGACGGTG 
CAGAATGTGG TCTTTGGTCA CTGTCCCTTG ACCCCGGGTC CCTGCAGCGA TCACGATCTT 
TTCACTCGAC TGCCAGACAA CTTTTGTCTG AATGATCCCT GTATGGGCCA TGGAACCTGC 
TCGTCCAGTC CAGAGGGATA CGAATGTCGG TGCACGGCAC GTTACTCGGG AAAAAATTGC 
CAGAAGGACA ATGGCTCGCC GTGTGCCAAA AATCCATGTG AGAACGGTGG TTCCTGCCTG 
GAGAATTCCC GAGGGGATTA CCAGTGCTTC TGTGATCCCA ACCACAGTGG TCAGCACTGC 
GAGACGGAGG TGAACATCCA CCCACTCTGC CAGACGAACC CCTGCCTGAA CAATGGAGCA 
TGTGTGGTGA TTGGCGGCAG TGGAGCACTC ACCTGCGAGT GTCCCAAAGG ATACGCTGGT 
GCCAGGTGCG AGGTGGACAC GGATGAGTGC GCCTCACAGC CGTGTCAGAA CAACGGAAGC 
TGCATAGACC GTATCAATGG ATTCAGCTGC GATTGCAGCG GCACCGGTTA CACGGGTGCG 
TTCTGCCAGA CGAATGTGGA TGAGTGCGAC AAGAATCCGT GCCTGAACGG CGGCAGATGC 
TTCGATACCT ACGGATGGTA CACCTGCCAG TGTCTGGATG GTTGGGGCGG CGAGATTTGT 
GATCGACCCA TGACATGCCA GACGCAGCAG TGTTTGAACG GTGGCACCTG CCTGGACAAG 
CCCATCGGCT TCCAGTGCCT CTGTCCGCCG GAGTACACTG GTGAACTGTG CCAAATCGCA 
CCCAGTTGCG CACAGCAGTG CCCCATCGAT TCGGAGTGCG TAGGCGGCAA ATGCGTTTGT 
AAGCCAGGCT CATCGGGTTA CAACTGTCAA ACGAGCACGG GCGATGGAGC TTCTGCTTTG 
GCCCTGACAC CCATCAACTG CAATGCCACC AATGGAAAGT GTCTCAATGG AGGAACTTGC 
TCCATGAACG GAACCCACTG CTATTGCGCC GTCGGCTATT CGGGAGATCG TTGCGAGAAG 
GCCGAGAACT GTTCGCCACT GAACTGCCAG GAACCGATGG TATGTGTCCA GAATCAGTGC 
CTCTGCCCGG AGAACAAGGT GTGCAACCAG TGCGCCACAC AACCGTGCCA GAATGGCGGT 
GAATGTGTGG ACCTACCGAA TGGAGACTAC GAGTGCAAGT GCACCCGTGG ATGGACTGGA 
CGCACCTGTG GCAACGACGT GGACGAGTGC ACCCTGCATC CGAAGATCTG CGGCAATGGT 
ATCTGCAAGA ACGAGAAGGG ATCGTACAAA TGCTACTGTA CGCCTGGATT TACCGGAGTC 
CACTGCGATT CCGATGTGGA CGAATGCCTT AGCTTTCCCT GCCTCAACGG AGCCACGTGC 
CACAACAAGA TAAATGCCTA CGAGTGCGTT TGCCAACCAG GATATGAGGG AGAGAACTGC 
GAAGTAGATA TCGATGAGTG CGGCAGTAAT CCCTGCTCGA ACGGATCCAC CTGCATCGAC 
AGGATCAACA ACTTCACCTG CAACTGCATT CCGGGAATGA CGGGCCGCAT ATGTGACATC 
GACATTGACG ACTGTGTGGG AGATCCCTGC TTGAATGGTG GTCAGTGCAT CGACCAATTG 
GGTGGCTTCC GTTGTGACTG CAGTGGCACC GGTTATGAGG GCGAGAATTG TGAGCTGAAC 
ATAGACGAAT GCCTCTCGAA TCCGTGCACG AATGGTGCCA AGTGCCTGGA CAGGGTTAAG 
GACTACTTCT GCGATTGCCA CAACGGCTAC AAGGGAAAGA ACTGTGAGCA GGACATCAAC 
GAGTGCGAGA GTAATCCCTG CCAGTACAAT GGCAACTGCC TAGAGCGTTC GAACATAACG 
CTTTACCAAA TGAGTAGGAT CACGGATCTG CCCAAGGTGT TTAGCCAGCC CTTTAGCTTT 
GAGAATGCCA GCGGCTACGA GTGCGTCTGT GTGCCCGGCA TCATTGGAAA GAACTGCGAG 
ATCAACATTA ATGAGTGCGA CAGTAATCCA TGCAGCAAGC ACGGAAATTG CAATGACGGG 
ATTGGAACCT ATACCTGCGA ATGCGAACCT GGCTTCGAGG GCACCCACTG TGAGATCAAC 
ATAGATGAAT GTGATCGCTA TAATCCCTGC CAGAGGGGCA CTTGCTATGA CCAGATAGAC 
GACTACGACT GCGACTGCGA TGCGAACTAT GGAGGCAAAA ACTGTTCCGT TCTTCTCAAA 
GGCTGTGACC AGAATCCTTG TTTAAATGGC GGTGCCTGCT TGCCGTATTT GATCAACGAG 
GTAACTCATC TGTACAACTG CACCTGCGAG AACGGCTTCC AGGGCGATAA ATGCGAGAAG 
ACCACCACTC TGTCCATGGT GGCCACTAGC CTGATTTCGG TTACCACGGA ACGCGAGGAA 
GGCTACGACA TTAATCTTCA ATTTAGGACC ACACTGCCCA ACGGAGTTTT GGCTTTCGGA 
ACCACCGGCG AAAAGAATGA ACCAGTTAGC TATATTTTGG AGCTGATTAA TGGACGACTG 
AATCTACATT CCTCGCTCCT AAATAAGTGG GAGGGCGTGT TTATTGGATC GAAGCTGAAC 
GATAGCAACT GGCACAAGGT GTTTGTGGCC ATAAACACTT CGCATCTAGT CCTTTCGGCC 
AACGACGAGC AGGCTATTTT TCCGGTTGGC TCCTATGAAA CGGCCAATAA TAGCCAGCCT 
TCATTCCCGC GCACATACCT CGGAGGCACC ATTCCCAATC TGAAGTCCTA TCTGCGCCAC 
CTCACCCACC AACCATCAGC TTTTGTGGGC TGCATGCAAG ATATAATGGT CAATGGTAAA 
TGGATCTTTC CCGATGAGCA GGATGCGAAT ATCAGTTATA CCAAACTGGA AAATGTCCAG 
AGCGGTTGTC CGCGCACCGA ACAATGTAAA CCGAATCCTT GCCATTCGAA CGGTGAATGC 
ACGGACCTTT GGCACACCTT CGCTTGTCAC TGTCCCAGAC CATTCTTTGG GCACACATGT 
CAGCACAATA TGACTGCTGC CACTTTTGGC CACGAGAACA CAACCCATTC GGCCGTGATT 
GTGGAGACCA CAGATGTGGC TAGGCGAGCT ATTAGATCCA TCCTAGACAT TTCCATGTTT 
ATTCGTACAC GCGAGCCAAC TGGTCAAGTC TTTTACTTGG GCACTGATCC ACGCAAAGCC 
CCCACTAAAA ATATTGGCGA CTCATATGTG GCCGCCAAAT TACACGGCGG CGAGCTGCTG 
GTAAAGATGC AGTTCAGCGG TACTCCGGAG GCCTACACCG TTGGTGGCCA GAAATTGGAC 
AACGGCTATA ACCACCTGAT CGAGGTGGTG CGCAATCAAA CGCTCGTGCA GGTCAAGCTC 
AATGGCACCG AGTACTTCCG CAAGACGCTG TCCACGACGG GTCTGCTGGA CGCACAGGTG 
CTTTACTTGG GCGGACCTGC ACCCACACGC GAGTCACTTC TGGGCGCGAC CACAGAACCA 
GGAATAATTC CAGTTCCGGG AGCAGGAATA CCCATTGAAG ACACAACAGT GCCCAAGGAG 
GCGGACGACA GCAGGGACTA CTTTAAGGGC ATAATCCAGG ACGTGAAGGT TAGCAATGGC 
TCGCTCAACC TGATTGTGGA GATGTATTCC CTAAACGTGA CAGATGTCCA AGTGAATGCC 
AAACCTCTGG GCGCTGTAAC CATCGATCGT GCCTCCGTGT TACCTGGTGA GGTGTCCGAT 
GATCTGTGCC GAAAGAATCC CTGCCTCCAT AATGCCGAGT GCAGGAACAC ATGGAATGAC 
TACACTTGCA AATGCCCAAA TGGTTACAAG GGAAAGAACT GCCAGGAGAT CGAATTTTGC 
CAACACGTTA CATGTCCGGG ACAGAGCTTG TGCCAAAATC TGGACGATGG CTATGAGTGT 
GTTACGAACA CCACATTTAC TGGGCAGGAA CGCAGTCCTC TCGCCTTCTT CTACTTCCAG 
GAGCAGCAAT CTGACGATAT TGTCAGTGAA GCGTCTCCCA AGCAAACTCT TAAACCGGTA 
ATTGATATTG CCTTCCGCAC TCGTGCTGGA GGTACTCTCC TGTACATAGA CAATGTGGAC 
GGATTCTTTG AAATTGGAGT AAACGGAGGA CGAGTGACCA TCACCTGGAA GCTAAGTGCA 
CTGCATTTTG GCGAGTCTGC ACGCTTTGAG AAGGAGAACA CTGACGGAGA ATGGAGTCGC 
ATTTACCTCA GGGCACACAA CAGCAAACTG GAGGGTGGAT GGAAGGGATG GGAATCGATG 
GTGGATCCGA CGCCAGCTTT TTCCACGGAC ATCGACCAAG CGGCCTTTCA GTCCCTGATC 
GCTACAAGTA CCCAGGTTTA CTTGGGTGGC ATGCCAGAGT CGCGACAAGC ACGAGGATCC 
ACTTTGTCTG CCCAGCAGGG CTCTCAGTTC AAGGGCTGTG TGGGAGAGGC AAGGGTGGGC 
GATCTTTTAC TGCCCTACTT CTCCATGGCG GAACTGTATT CGCGCACCAA TGTTTCAGTA 
CAGCAAAAGG CTCAATTCCG TCTAAATGCC ACACGACCTG AGGAGGGCTG CATCCTGTGC 
TTCCAGTCAG ACTGCAAAAA TGACGGCTTC TGTCAATCTC CTTCAGATGA GTACGCCTGC 
ACCTGTCAGC CTGGATTCGA GGGCGATGAT TGCGGCACGG ACATCGACGA GTGTCTTAAC 
ACGGAATGCT TGAACAACGG CACCTGCATC AACCAGGTTG CAGCTTTCTT CTGCCAGTGT 
CAGCCAGGAT TCGAGGGTCA GCACTGTGAG CAAAACATCG ACGAGTGTGC GGATCAGCCG 
TGCCACAACG GTGGCAACTG CACGGATCTT ATCGCATCGT ACGTGTGCGA CTGCCCTGAG 
GACTATATGG GCCCGCAGTG CGACGTGCTG AAGCAAATGA CCTGCGAGAA CGAGCCATGT 
CGGAATGGAT CAACCTGCCA GAATGGATTC AATGCTTCTA CTGGCAATAA CTTTACATGT 
ACATGCGTGC CCGGCTTCGA GGGTCCACTG TGTGACATAC CCTTCTGTGA AATAACGCCT 
TGCGATAACG GTGGCCTCTG CCTGACCACT GGAGCGGTAC CGATGTGCAA ATGTAGTCTG 
GGATACACTG GTCGCCTGTG CGAGCAGGAC ATTAACGAGT GCGAATCGAA TCCATGCCAG 
AACGGTGGTC AGTGTAAGGA CCTCGTCGGC AGGTACGAGT GCGATTGCCA GGGCACCGGG 
TTCGAGGGCA TTCGCTGTGA AAATGACATC GACGAGTGCA ACATGGAGGG AGATTACTGC 
GGCGGATTGG GCCGGTGTTT CAACAAGCCC GGATCCTTCC AGTGCATCTG CCAGAAACCC 
TATTGCGGAG CCTACTGCAA CTTTACGGAT CCCTGCAACG CTACGGACCT CTGCTCAAAC 
GGCGGTCGCT GCGTAGAGTC CTGCGGCGCC AAACCGGACT ACTACTGCGA GTGTCCGGAA 
GGTTTCGCGG GAAAGAATTG CACAGCACCG ATTACGGCCA AGGAGGACGG GCCTTCGACC 
ACAGACATTG CCATCATTGT AATACCCGTA GTGGTGGTGC TGCTGCTGAT CGCGGGAGCC 
CTCCTGGGCA CCTTCCTGGT GATGGCCAGG AACAAGCGAG CAACCAGGGG CACCTATAGC 
CCGAGCGCGC AAGAGTACTG CAACCCACGG CTGGAAATGG ACAACGTACT GAAGCCACCG 
CCGGAAGAGC GACTAATTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524480.2
CDS	550..6990
Translation	MAKIANASLSQQQKQRQAETATTTTTTVAASVETATTTARSRDRTKSAAQITSHLLKRAISVYSSPQWIPLFILIYLATDVASVAVPTKEAYFNGSTYLRLTTPMPIWDHSAISFRSCRGGEILAQQYNKNSIVISVLNDFLQISLAGPAVHGPNNRLDVKLPYQLLDNRWHTLQFKYEYGNLYLHVDRAASIFANSTYNSQFLTNQDIGYKDAILILGNSFSGCLLDGPGLQFVNNSTVQNVVFGHCPLTPGPCSDHDLFTRLPDNFCLNDPCMGHGTCSSSPEGYECRCTARYSGKNCQKDNGSPCAKNPCENGGSCLENSRGDYQCFCDPNHSGQHCETEVNIHPLCQTNPCLNNGACVVIGGSGALTCECPKGYAGARCEVDTDECASQPCQNNGSCIDRINGFSCDCSGTGYTGAFCQTNVDECDKNPCLNGGRCFDTYGWYTCQCLDGWGGEICDRPMTCQTQQCLNGGTCLDKPIGFQCLCPPEYTGELCQIAPSCAQQCPIDSECVGGKCVCKPGSSGYNCQTSTGDGASALALTPINCNATNGKCLNGGTCSMNGTHCYCAVGYSGDRCEKAENCSPLNCQEPMVCVQNQCLCPENKVCNQCATQPCQNGGECVDLPNGDYECKCTRGWTGRTCGNDVDECTLHPKICGNGICKNEKGSYKCYCTPGFTGVHCDSDVDECLSFPCLNGATCHNKINAYECVCQPGYEGENCEVDIDECGSNPCSNGSTCIDRINNFTCNCIPGMTGRICDIDIDDCVGDPCLNGGQCIDQLGGFRCDCSGTGYEGENCELNIDECLSNPCTNGAKCLDRVKDYFCDCHNGYKGKNCEQDINECESNPCQYNGNCLERSNITLYQMSRITDLPKVFSQPFSFENASGYECVCVPGIIGKNCEININECDSNPCSKHGNCNDGIGTYTCECEPGFEGTHCEINIDECDRYNPCQRGTCYDQIDDYDCDCDANYGGKNCSVLLKGCDQNPCLNGGACLPYLINEVTHLYNCTCENGFQGDKCEKTTTLSMVATSLISVTTEREEGYDINLQFRTTLPNGVLAFGTTGEKNEPVSYILELINGRLNLHSSLLNKWEGVFIGSKLNDSNWHKVFVAINTSHLVLSANDEQAIFPVGSYETANNSQPSFPRTYLGGTIPNLKSYLRHLTHQPSAFVGCMQDIMVNGKWIFPDEQDANISYTKLENVQSGCPRTEQCKPNPCHSNGECTDLWHTFACHCPRPFFGHTCQHNMTAATFGHENTTHSAVIVETTDVARRAIRSILDISMFIRTREPTGQVFYLGTDPRKAPTKNIGDSYVAAKLHGGELLVKMQFSGTPEAYTVGGQKLDNGYNHLIEVVRNQTLVQVKLNGTEYFRKTLSTTGLLDAQVLYLGGPAPTRESLLGATTEPGIIPVPGAGIPIEDTTVPKEADDSRDYFKGIIQDVKVSNGSLNLIVEMYSLNVTDVQVNAKPLGAVTIDRASVLPGEVSDDLCRKNPCLHNAECRNTWNDYTCKCPNGYKGKNCQEIEFCQHVTCPGQSLCQNLDDGYECVTNTTFTGQERSPLAFFYFQEQQSDDIVSEASPKQTLKPVIDIAFRTRAGGTLLYIDNVDGFFEIGVNGGRVTITWKLSALHFGESARFEKENTDGEWSRIYLRAHNSKLEGGWKGWESMVDPTPAFSTDIDQAAFQSLIATSTQVYLGGMPESRQARGSTLSAQQGSQFKGCVGEARVGDLLLPYFSMAELYSRTNVSVQQKAQFRLNATRPEEGCILCFQSDCKNDGFCQSPSDEYACTCQPGFEGDDCGTDIDECLNTECLNNGTCINQVAAFFCQCQPGFEGQHCEQNIDECADQPCHNGGNCTDLIASYVCDCPEDYMGPQCDVLKQMTCENEPCRNGSTCQNGFNASTGNNFTCTCVPGFEGPLCDIPFCEITPCDNGGLCLTTGAVPMCKCSLGYTGRLCEQDINECESNPCQNGGQCKDLVGRYECDCQGTGFEGIRCENDIDECNMEGDYCGGLGRCFNKPGSFQCICQKPYCGAYCNFTDPCNATDLCSNGGRCVESCGAKPDYYCECPEGFAGKNCTAPITAKEDGPSTTDIAIIVIPVVVVLLLIAGALLGTFLVMARNKRATRGTYSPSAQEYCNPRLEMDNVLKPPPEERLI

Target ORF information:

RefSeq Version	NM_079756.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster crumbs (crb), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079756.3

ORF Insert Sequence:

ATGGCTAAAA TCGCCAATGC GTCACTGTCG CAACAACAAA AACAGCGGCA AGCAGAAACA 
GCAACGACCA CAACAACAAC AGTGGCGGCA TCAGTAGAAA CAGCAACAAC AACAGCAAGA 
AGTCGAGACC GCACGAAAAG CGCAGCTCAA ATTACGTCGC ATTTGCTCAA GCGCGCCATT 
TCAGTTTATT CGTCGCCTCA ATGGATACCG CTCTTCATAC TTATTTACTT AGCAACAGAT 
GTCGCCTCAG TGGCGGTGCC GACGAAGGAG GCGTACTTTA ATGGCTCCAC TTACCTCCGC 
CTCACCACGC CGATGCCCAT TTGGGATCAC TCGGCGATTA GTTTCCGCTC GTGCCGCGGC 
GGCGAGATCC TCGCCCAGCA GTACAACAAG AACTCCATTG TAATCTCAGT GCTCAATGAC 
TTTCTGCAAA TCTCACTGGC TGGACCCGCC GTCCATGGGC CCAACAACCG GCTGGATGTC 
AAGCTGCCCT ACCAACTGCT GGACAACCGC TGGCATACGC TGCAGTTCAA GTACGAGTAC 
GGAAATCTCT ACCTGCATGT GGATCGCGCG GCAAGCATAT TTGCCAACTC CACGTACAAC 
AGTCAGTTCC TGACTAACCA GGACATTGGC TACAAGGACG CCATCTTGAT ACTGGGAAAC 
TCCTTCTCCG GTTGCCTTCT GGATGGACCA GGTCTGCAAT TTGTGAACAA CTCGACGGTG 
CAGAATGTGG TCTTTGGTCA CTGTCCCTTG ACCCCGGGTC CCTGCAGCGA TCACGATCTT 
TTCACTCGAC TGCCAGACAA CTTTTGTCTG AATGATCCCT GTATGGGCCA TGGAACCTGC 
TCGTCCAGTC CAGAGGGATA CGAATGTCGG TGCACGGCAC GTTACTCGGG AAAAAATTGC 
CAGAAGGACA ATGGCTCGCC GTGTGCCAAA AATCCATGTG AGAACGGTGG TTCCTGCCTG 
GAGAATTCCC GAGGGGATTA CCAGTGCTTC TGTGATCCCA ACCACAGTGG TCAGCACTGC 
GAGACGGAGG TGAACATCCA CCCACTCTGC CAGACGAACC CCTGCCTGAA CAATGGAGCA 
TGTGTGGTGA TTGGCGGCAG TGGAGCACTC ACCTGCGAGT GTCCCAAAGG ATACGCTGGT 
GCCAGGTGCG AGGTGGACAC GGATGAGTGC GCCTCACAGC CGTGTCAGAA CAACGGAAGC 
TGCATAGACC GTATCAATGG ATTCAGCTGC GATTGCAGCG GCACCGGTTA CACGGGTGCG 
TTCTGCCAGA CGAATGTGGA TGAGTGCGAC AAGAATCCGT GCCTGAACGG CGGCAGATGC 
TTCGATACCT ACGGATGGTA CACCTGCCAG TGTCTGGATG GTTGGGGCGG CGAGATTTGT 
GATCGACCCA TGACATGCCA GACGCAGCAG TGTTTGAACG GTGGCACCTG CCTGGACAAG 
CCCATCGGCT TCCAGTGCCT CTGTCCGCCG GAGTACACTG GTGAACTGTG CCAAATCGCA 
CCCAGTTGCG CACAGCAGTG CCCCATCGAT TCGGAGTGCG TAGGCGGCAA ATGCGTTTGT 
AAGCCAGGCT CATCGGGTTA CAACTGTCAA ACGAGCACGG GCGATGGAGC TTCTGCTTTG 
GCCCTGACAC CCATCAACTG CAATGCCACC AATGGAAAGT GTCTCAATGG AGGAACTTGC 
TCCATGAACG GAACCCACTG CTATTGCGCC GTCGGCTATT CGGGAGATCG TTGCGAGAAG 
GCCGAGAACT GTTCGCCACT GAACTGCCAG GAACCGATGG TATGTGTCCA GAATCAGTGC 
CTCTGCCCGG AGAACAAGGT GTGCAACCAG TGCGCCACAC AACCGTGCCA GAATGGCGGT 
GAATGTGTGG ACCTACCGAA TGGAGACTAC GAGTGCAAGT GCACCCGTGG ATGGACTGGA 
CGCACCTGTG GCAACGACGT GGACGAGTGC ACCCTGCATC CGAAGATCTG CGGCAATGGT 
ATCTGCAAGA ACGAGAAGGG ATCGTACAAA TGCTACTGTA CGCCTGGATT TACCGGAGTC 
CACTGCGATT CCGATGTGGA CGAATGCCTT AGCTTTCCCT GCCTCAACGG AGCCACGTGC 
CACAACAAGA TAAATGCCTA CGAGTGCGTT TGCCAACCAG GATATGAGGG AGAGAACTGC 
GAAGTAGATA TCGATGAGTG CGGCAGTAAT CCCTGCTCGA ACGGATCCAC CTGCATCGAC 
AGGATCAACA ACTTCACCTG CAACTGCATT CCGGGAATGA CGGGCCGCAT ATGTGACATC 
GACATTGACG ACTGTGTGGG AGATCCCTGC TTGAATGGTG GTCAGTGCAT CGACCAATTG 
GGTGGCTTCC GTTGTGACTG CAGTGGCACC GGTTATGAGG GCGAGAATTG TGAGCTGAAC 
ATAGACGAAT GCCTCTCGAA TCCGTGCACG AATGGTGCCA AGTGCCTGGA CAGGGTTAAG 
GACTACTTCT GCGATTGCCA CAACGGCTAC AAGGGAAAGA ACTGTGAGCA GGACATCAAC 
GAGTGCGAGA GTAATCCCTG CCAGTACAAT GGCAACTGCC TAGAGCGTTC GAACATAACG 
CTTTACCAAA TGAGTAGGAT CACGGATCTG CCCAAGGTGT TTAGCCAGCC CTTTAGCTTT 
GAGAATGCCA GCGGCTACGA GTGCGTCTGT GTGCCCGGCA TCATTGGAAA GAACTGCGAG 
ATCAACATTA ATGAGTGCGA CAGTAATCCA TGCAGCAAGC ACGGAAATTG CAATGACGGG 
ATTGGAACCT ATACCTGCGA ATGCGAACCT GGCTTCGAGG GCACCCACTG TGAGATCAAC 
ATAGATGAAT GTGATCGCTA TAATCCCTGC CAGAGGGGCA CTTGCTATGA CCAGATAGAC 
GACTACGACT GCGACTGCGA TGCGAACTAT GGAGGCAAAA ACTGTTCCGT TCTTCTCAAA 
GGCTGTGACC AGAATCCTTG TTTAAATGGC GGTGCCTGCT TGCCGTATTT GATCAACGAG 
GTAACTCATC TGTACAACTG CACCTGCGAG AACGGCTTCC AGGGCGATAA ATGCGAGAAG 
ACCACCACTC TGTCCATGGT GGCCACTAGC CTGATTTCGG TTACCACGGA ACGCGAGGAA 
GGCTACGACA TTAATCTTCA ATTTAGGACC ACACTGCCCA ACGGAGTTTT GGCTTTCGGA 
ACCACCGGCG AAAAGAATGA ACCAGTTAGC TATATTTTGG AGCTGATTAA TGGACGACTG 
AATCTACATT CCTCGCTCCT AAATAAGTGG GAGGGCGTGT TTATTGGATC GAAGCTGAAC 
GATAGCAACT GGCACAAGGT GTTTGTGGCC ATAAACACTT CGCATCTAGT CCTTTCGGCC 
AACGACGAGC AGGCTATTTT TCCGGTTGGC TCCTATGAAA CGGCCAATAA TAGCCAGCCT 
TCATTCCCGC GCACATACCT CGGAGGCACC ATTCCCAATC TGAAGTCCTA TCTGCGCCAC 
CTCACCCACC AACCATCAGC TTTTGTGGGC TGCATGCAAG ATATAATGGT CAATGGTAAA 
TGGATCTTTC CCGATGAGCA GGATGCGAAT ATCAGTTATA CCAAACTGGA AAATGTCCAG 
AGCGGTTGTC CGCGCACCGA ACAATGTAAA CCGAATCCTT GCCATTCGAA CGGTGAATGC 
ACGGACCTTT GGCACACCTT CGCTTGTCAC TGTCCCAGAC CATTCTTTGG GCACACATGT 
CAGCACAATA TGACTGCTGC CACTTTTGGC CACGAGAACA CAACCCATTC GGCCGTGATT 
GTGGAGACCA CAGATGTGGC TAGGCGAGCT ATTAGATCCA TCCTAGACAT TTCCATGTTT 
ATTCGTACAC GCGAGCCAAC TGGTCAAGTC TTTTACTTGG GCACTGATCC ACGCAAAGCC 
CCCACTAAAA ATATTGGCGA CTCATATGTG GCCGCCAAAT TACACGGCGG CGAGCTGCTG 
GTAAAGATGC AGTTCAGCGG TACTCCGGAG GCCTACACCG TTGGTGGCCA GAAATTGGAC 
AACGGCTATA ACCACCTGAT CGAGGTGGTG CGCAATCAAA CGCTCGTGCA GGTCAAGCTC 
AATGGCACCG AGTACTTCCG CAAGACGCTG TCCACGACGG GTCTGCTGGA CGCACAGGTG 
CTTTACTTGG GCGGACCTGC ACCCACACGC GAGTCACTTC TGGGCGCGAC CACAGAACCA 
GGAATAATTC CAGTTCCGGG AGCAGGAATA CCCATTGAAG ACACAACAGT GCCCAAGGAG 
GCGGACGACA GCAGGGACTA CTTTAAGGGC ATAATCCAGG ACGTGAAGGT TAGCAATGGC 
TCGCTCAACC TGATTGTGGA GATGTATTCC CTAAACGTGA CAGATGTCCA AGTGAATGCC 
AAACCTCTGG GCGCTGTAAC CATCGATCGT GCCTCCGTGT TACCTGGTGA GGTGTCCGAT 
GATCTGTGCC GAAAGAATCC CTGCCTCCAT AATGCCGAGT GCAGGAACAC ATGGAATGAC 
TACACTTGCA AATGCCCAAA TGGTTACAAG GGAAAGAACT GCCAGGAGAT CGAATTTTGC 
CAACACGTTA CATGTCCGGG ACAGAGCTTG TGCCAAAATC TGGACGATGG CTATGAGTGT 
GTTACGAACA CCACATTTAC TGGGCAGGAA CGCAGTCCTC TCGCCTTCTT CTACTTCCAG 
GAGCAGCAAT CTGACGATAT TGTCAGTGAA GCGTCTCCCA AGCAAACTCT TAAACCGGTA 
ATTGATATTG CCTTCCGCAC TCGTGCTGGA GGTACTCTCC TGTACATAGA CAATGTGGAC 
GGATTCTTTG AAATTGGAGT AAACGGAGGA CGAGTGACCA TCACCTGGAA GCTAAGTGCA 
CTGCATTTTG GCGAGTCTGC ACGCTTTGAG AAGGAGAACA CTGACGGAGA ATGGAGTCGC 
ATTTACCTCA GGGCACACAA CAGCAAACTG GAGGGTGGAT GGAAGGGATG GGAATCGATG 
GTGGATCCGA CGCCAGCTTT TTCCACGGAC ATCGACCAAG CGGCCTTTCA GTCCCTGATC 
GCTACAAGTA CCCAGGTTTA CTTGGGTGGC ATGCCAGAGT CGCGACAAGC ACGAGGATCC 
ACTTTGTCTG CCCAGCAGGG CTCTCAGTTC AAGGGCTGTG TGGGAGAGGC AAGGGTGGGC 
GATCTTTTAC TGCCCTACTT CTCCATGGCG GAACTGTATT CGCGCACCAA TGTTTCAGTA 
CAGCAAAAGG CTCAATTCCG TCTAAATGCC ACACGACCTG AGGAGGGCTG CATCCTGTGC 
TTCCAGTCAG ACTGCAAAAA TGACGGCTTC TGTCAATCTC CTTCAGATGA GTACGCCTGC 
ACCTGTCAGC CTGGATTCGA GGGCGATGAT TGCGGCACGG ACATCGACGA GTGTCTTAAC 
ACGGAATGCT TGAACAACGG CACCTGCATC AACCAGGTTG CAGCTTTCTT CTGCCAGTGT 
CAGCCAGGAT TCGAGGGTCA GCACTGTGAG CAAAACATCG ACGAGTGTGC GGATCAGCCG 
TGCCACAACG GTGGCAACTG CACGGATCTT ATCGCATCGT ACGTGTGCGA CTGCCCTGAG 
GACTATATGG GCCCGCAGTG CGACGTGCTG AAGCAAATGA CCTGCGAGAA CGAGCCATGT 
CGGAATGGAT CAACCTGCCA GAATGGATTC AATGCTTCTA CTGGCAATAA CTTTACATGT 
ACATGCGTGC CCGGCTTCGA GGGTCCACTG TGTGACATAC CCTTCTGTGA AATAACGCCT 
TGCGATAACG GTGGCCTCTG CCTGACCACT GGAGCGGTAC CGATGTGCAA ATGTAGTCTG 
GGATACACTG GTCGCCTGTG CGAGCAGGAC ATTAACGAGT GCGAATCGAA TCCATGCCAG 
AACGGTGGTC AGTGTAAGGA CCTCGTCGGC AGGTACGAGT GCGATTGCCA GGGCACCGGG 
TTCGAGGGCA TTCGCTGTGA AAATGACATC GACGAGTGCA ACATGGAGGG AGATTACTGC 
GGCGGATTGG GCCGGTGTTT CAACAAGCCC GGATCCTTCC AGTGCATCTG CCAGAAACCC 
TATTGCGGAG CCTACTGCAA CTTTACGGAT CCCTGCAACG CTACGGACCT CTGCTCAAAC 
GGCGGTCGCT GCGTAGAGTC CTGCGGCGCC AAACCGGACT ACTACTGCGA GTGTCCGGAA 
GGTTTCGCGG GAAAGAATTG CACAGCACCG ATTACGGCCA AGGAGGACGG GCCTTCGACC 
ACAGACATTG CCATCATTGT AATACCCGTA GTGGTGGTGC TGCTGCTGAT CGCGGGAGCC 
CTCCTGGGCA CCTTCCTGGT GATGGCCAGG AACAAGCGAG CAACCAGGGG CACCTATAGC 
CCGAGCGCGC AAGAGTACTG CAACCCACGG CTGGAAATGG ACAACGTACT GAAGCCACCG 
CCGGAAGAGC GACTAATTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crb ( NM_001260355.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247284.1 Drosophila melanogaster crumbs (crb), transcript variant C, mRNA.

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CloneID	OFa08721
Clone ID Related Accession (Same CDS sequence)	NM_001260355.2
Accession Version	NM_001260355.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	crumbs, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260355.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTAAAA TCGCCAATGC GTCACTGTCG CAACAACAAA AACAGCGGCA AGCAGAAACA 
GCAACGACCA CAACAACAAC AGTGGCGGCA TCAGTAGAAA CAGCAACAAC AACAGCAAGA 
AGTCGAGACC GCACGAAAAG CGCAGCTCAA ATTACGTCGC ATTTGCTCAA GCGCGCCATT 
TCAGTTTATT CGTCGCCTCA ATGGATACCG CTCTTCATAC TTATTTACTT AGCAACAGAT 
GTCGCCTCAG TGGCGGTGCC GACGAAGGAG GCGTACTTTA ATGGCTCCAC TTACCTCCGC 
CTCACCACGC CGATGCCCAT TTGGGATCAC TCGGCGATTA GTTTCCGCTC GTGCCGCGGC 
GGCGAGATCC TCGCCCAGCA GTACAACAAG AACTCCATTG TAATCTCAGT GCTCAATGAC 
TTTCTGCAAA TCTCACTGGC TGGACCCGCC GTCCATGGGC CCAACAACCG GCTGGATGTC 
AAGCTGCCCT ACCAACTGCT GGACAACCGC TGGCATACGC TGCAGTTCAA GTACGAGTAC 
GGAAATCTCT ACCTGCATGT GGATCGCGCG GCAAGCATAT TTGCCAACTC CACGTACAAC 
AGTCAGTTCC TGACTAACCA GGACATTGGC TACAAGGACG CCATCTTGAT ACTGGGAAAC 
TCCTTCTCCG GTTGCCTTCT GGATGGACCA GGTCTGCAAT TTGTGAACAA CTCGACGGTG 
CAGAATGTGG TCTTTGGTCA CTGTCCCTTG ACCCCGGGTC CCTGCAGCGA TCACGATCTT 
TTCACTCGAC TGCCAGACAA CTTTTGTCTG AATGATCCCT GTATGGGCCA TGGAACCTGC 
TCGTCCAGTC CAGAGGGATA CGAATGTCGG TGCACGGCAC GTTACTCGGG AAAAAATTGC 
CAGAAGGACA ATGGCTCGCC GTGTGCCAAA AATCCATGTG AGAACGGTGG TTCCTGCCTG 
GAGAATTCCC GAGGGGATTA CCAGTGCTTC TGTGATCCCA ACCACAGTGG TCAGCACTGC 
GAGACGGAGG TGAACATCCA CCCACTCTGC CAGACGAACC CCTGCCTGAA CAATGGAGCA 
TGTGTGGTGA TTGGCGGCAG TGGAGCACTC ACCTGCGAGT GTCCCAAAGG ATACGCTGGT 
GCCAGGTGCG AGGTGGACAC GGATGAGTGC GCCTCACAGC CGTGTCAGAA CAACGGAAGC 
TGCATAGACC GTATCAATGG ATTCAGCTGC GATTGCAGCG GCACCGGTTA CACGGGTGCG 
TTCTGCCAGA CGAATGTGGA TGAGTGCGAC AAGAATCCGT GCCTGAACGG CGGCAGATGC 
TTCGATACCT ACGGATGGTA CACCTGCCAG TGTCTGGATG GTTGGGGCGG CGAGATTTGT 
GATCGACCCA TGACATGCCA GACGCAGCAG TGTTTGAACG GTGGCACCTG CCTGGACAAG 
CCCATCGGCT TCCAGTGCCT CTGTCCGCCG GAGTACACTG GTGAACTGTG CCAAATCGCA 
CCCAGTTGCG CACAGCAGTG CCCCATCGAT TCGGAGTGCG TAGGCGGCAA ATGCGTTTGT 
AAGCCAGGCT CATCGGGCCC CATTGGTCAC TGCCTGCCAA CAACAACCAC ACCGACACCA 
GAACAAGAGC CAACAACAAC GCCAAGAACC ACACCAAACC CTAACCCAGC TATTCCCAAC 
ACCCTAACAA CAACAACCAA GATACCACCA ATAACAACCA GCCGAACACT GGTGGGAACG 
ACCACGGGTA GCAGGAGACC ACCACAACAG CCACTACAAT CTCCGACCCA ACGCTCGGCC 
TCCCTGAATG CATGTCCCCA AGAAAACTGC TTGAACGGTG GCACCTGCCT GGGCTATAGT 
GGCAATTATT CCTGTATTTG TGCTAGTGGA TACACAGGTT ACAACTGTCA AACGAGCACG 
GGCGATGGAG CTTCTGCTTT GGCCCTGACA CCCATCAACT GCAATGCCAC CAATGGAAAG 
TGTCTCAATG GAGGAACTTG CTCCATGAAC GGAACCCACT GCTATTGCGC CGTCGGCTAT 
TCGGGAGATC GTTGCGAGAA GGCCGAGAAC TGTTCGCCAC TGAACTGCCA GGAACCGATG 
GTATGTGTCC AGAATCAGTG CCTCTGCCCG GAGAACAAGG TGTGCAACCA GTGCGCCACA 
CAACCGTGCC AGAATGGCGG TGAATGTGTG GACCTACCGA ATGGAGACTA CGAGTGCAAG 
TGCACCCGTG GATGGACTGG ACGCACCTGT GGCAACGACG TGGACGAGTG CACCCTGCAT 
CCGAAGATCT GCGGCAATGG TATCTGCAAG AACGAGAAGG GATCGTACAA ATGCTACTGT 
ACGCCTGGAT TTACCGGAGT CCACTGCGAT TCCGATGTGG ACGAATGCCT TAGCTTTCCC 
TGCCTCAACG GAGCCACGTG CCACAACAAG ATAAATGCCT ACGAGTGCGT TTGCCAACCA 
GGATATGAGG GAGAGAACTG CGAAGTAGAT ATCGATGAGT GCGGCAGTAA TCCCTGCTCG 
AACGGATCCA CCTGCATCGA CAGGATCAAC AACTTCACCT GCAACTGCAT TCCGGGAATG 
ACGGGCCGCA TATGTGACAT CGACATTGAC GACTGTGTGG GAGATCCCTG CTTGAATGGT 
GGTCAGTGCA TCGACCAATT GGGTGGCTTC CGTTGTGACT GCAGTGGCAC CGGTTATGAG 
GGCGAGAATT GTGAGCTGAA CATAGACGAA TGCCTCTCGA ATCCGTGCAC GAATGGTGCC 
AAGTGCCTGG ACAGGGTTAA GGACTACTTC TGCGATTGCC ACAACGGCTA CAAGGGAAAG 
AACTGTGAGC AGGACATCAA CGAGTGCGAG AGTAATCCCT GCCAGTACAA TGGCAACTGC 
CTAGAGCGTT CGAACATAAC GCTTTACCAA ATGAGTAGGA TCACGGATCT GCCCAAGGTG 
TTTAGCCAGC CCTTTAGCTT TGAGAATGCC AGCGGCTACG AGTGCGTCTG TGTGCCCGGC 
ATCATTGGAA AGAACTGCGA GATCAACATT AATGAGTGCG ACAGTAATCC ATGCAGCAAG 
CACGGAAATT GCAATGACGG GATTGGAACC TATACCTGCG AATGCGAACC TGGCTTCGAG 
GGCACCCACT GTGAGATCAA CATAGATGAA TGTGATCGCT ATAATCCCTG CCAGAGGGGC 
ACTTGCTATG ACCAGATAGA CGACTACGAC TGCGACTGCG ATGCGAACTA TGGAGGCAAA 
AACTGTTCCG TTCTTCTCAA AGGCTGTGAC CAGAATCCTT GTTTAAATGG CGGTGCCTGC 
TTGCCGTATT TGATCAACGA GGTAACTCAT CTGTACAACT GCACCTGCGA GAACGGCTTC 
CAGGGCGATA AATGCGAGAA GACCACCACT CTGTCCATGG TGGCCACTAG CCTGATTTCG 
GTTACCACGG AACGCGAGGA AGGCTACGAC ATTAATCTTC AATTTAGGAC CACACTGCCC 
AACGGAGTTT TGGCTTTCGG AACCACCGGC GAAAAGAATG AACCAGTTAG CTATATTTTG 
GAGCTGATTA ATGGACGACT GAATCTACAT TCCTCGCTCC TAAATAAGTG GGAGGGCGTG 
TTTATTGGAT CGAAGCTGAA CGATAGCAAC TGGCACAAGG TGTTTGTGGC CATAAACACT 
TCGCATCTAG TCCTTTCGGC CAACGACGAG CAGGCTATTT TTCCGGTTGG CTCCTATGAA 
ACGGCCAATA ATAGCCAGCC TTCATTCCCG CGCACATACC TCGGAGGCAC CATTCCCAAT 
CTGAAGTCCT ATCTGCGCCA CCTCACCCAC CAACCATCAG CTTTTGTGGG CTGCATGCAA 
GATATAATGG TCAATGGTAA ATGGATCTTT CCCGATGAGC AGGATGCGAA TATCAGTTAT 
ACCAAACTGG AAAATGTCCA GAGCGGTTGT CCGCGCACCG AACAATGTAA ACCGAATCCT 
TGCCATTCGA ACGGTGAATG CACGGACCTT TGGCACACCT TCGCTTGTCA CTGTCCCAGA 
CCATTCTTTG GGCACACATG TCAGCACAAT ATGACTGCTG CCACTTTTGG CCACGAGAAC 
ACAACCCATT CGGCCGTGAT TGTGGAGACC ACAGATGTGG CTAGGCGAGC TATTAGATCC 
ATCCTAGACA TTTCCATGTT TATTCGTACA CGCGAGCCAA CTGGTCAAGT CTTTTACTTG 
GGCACTGATC CACGCAAAGC CCCCACTAAA AATATTGGCG ACTCATATGT GGCCGCCAAA 
TTACACGGCG GCGAGCTGCT GGTAAAGATG CAGTTCAGCG GTACTCCGGA GGCCTACACC 
GTTGGTGGCC AGAAATTGGA CAACGGCTAT AACCACCTGA TCGAGGTGGT GCGCAATCAA 
ACGCTCGTGC AGGTCAAGCT CAATGGCACC GAGTACTTCC GCAAGACGCT GTCCACGACG 
GGTCTGCTGG ACGCACAGGT GCTTTACTTG GGCGGACCTG CACCCACACG CGAGTCACTT 
CTGGGCGCGA CCACAGAACC AGGAATAATT CCAGTTCCGG GAGCAGGAAT ACCCATTGAA 
GACACAACAG TGCCCAAGGA GGCGGACGAC AGCAGGGACT ACTTTAAGGG CATAATCCAG 
GACGTGAAGG TTAGCAATGG CTCGCTCAAC CTGATTGTGG AGATGTATTC CCTAAACGTG 
ACAGATGTCC AAGTGAATGC CAAACCTCTG GGCGCTGTAA CCATCGATCG TGCCTCCGTG 
TTACCTGGTG AGGTGTCCGA TGATCTGTGC CGAAAGAATC CCTGCCTCCA TAATGCCGAG 
TGCAGGAACA CATGGAATGA CTACACTTGC AAATGCCCAA ATGGTTACAA GGGAAAGAAC 
TGCCAGGAGA TCGAATTTTG CCAACACGTT ACATGTCCGG GACAGAGCTT GTGCCAAAAT 
CTGGACGATG GCTATGAGTG TGTTACGAAC ACCACATTTA CTGGGCAGGA ACGCAGTCCT 
CTCGCCTTCT TCTACTTCCA GGAGCAGCAA TCTGACGATA TTGTCAGTGA AGCGTCTCCC 
AAGCAAACTC TTAAACCGGT AATTGATATT GCCTTCCGCA CTCGTGCTGG AGGTACTCTC 
CTGTACATAG ACAATGTGGA CGGATTCTTT GAAATTGGAG TAAACGGAGG ACGAGTGACC 
ATCACCTGGA AGCTAAGTGC ACTGCATTTT GGCGAGTCTG CACGCTTTGA GAAGGAGAAC 
ACTGACGGAG AATGGAGTCG CATTTACCTC AGGGCACACA ACAGCAAACT GGAGGGTGGA 
TGGAAGGGAT GGGAATCGAT GGTGGATCCG ACGCCAGCTT TTTCCACGGA CATCGACCAA 
GCGGCCTTTC AGTCCCTGAT CGCTACAAGT ACCCAGGTTT ACTTGGGTGG CATGCCAGAG 
TCGCGACAAG CACGAGGATC CACTTTGTCT GCCCAGCAGG GCTCTCAGTT CAAGGGCTGT 
GTGGGAGAGG CAAGGGTGGG CGATCTTTTA CTGCCCTACT TCTCCATGGC GGAACTGTAT 
TCGCGCACCA ATGTTTCAGT ACAGCAAAAG GCTCAATTCC GTCTAAATGC CACACGACCT 
GAGGAGGGCT GCATCCTGTG CTTCCAGTCA GACTGCAAAA ATGACGGCTT CTGTCAATCT 
CCTTCAGATG AGTACGCCTG CACCTGTCAG CCTGGATTCG AGGGCGATGA TTGCGGCACG 
GACATCGACG AGTGTCTTAA CACGGAATGC TTGAACAACG GCACCTGCAT CAACCAGGTT 
GCAGCTTTCT TCTGCCAGTG TCAGCCAGGA TTCGAGGGTC AGCACTGTGA GCAAAACATC 
GACGAGTGTG CGGATCAGCC GTGCCACAAC GGTGGCAACT GCACGGATCT TATCGCATCG 
TACGTGTGCG ACTGCCCTGA GGACTATATG GGCCCGCAGT GCGACGTGCT GAAGCAAATG 
ACCTGCGAGA ACGAGCCATG TCGGAATGGA TCAACCTGCC AGAATGGATT CAATGCTTCT 
ACTGGCAATA ACTTTACATG TACATGCGTG CCCGGCTTCG AGGGTCCACT GTGTGACATA 
CCCTTCTGTG AAATAACGCC TTGCGATAAC GGTGGCCTCT GCCTGACCAC TGGAGCGGTA 
CCGATGTGCA AATGTAGTCT GGGATACACT GGTCGCCTGT GCGAGCAGGA CATTAACGAG 
TGCGAATCGA ATCCATGCCA GAACGGTGGT CAGTGTAAGG ACCTCGTCGG CAGGTACGAG 
TGCGATTGCC AGGGCACCGG GTTCGAGGGC ATTCGCTGTG AAAATGACAT CGACGAGTGC 
AACATGGAGG GAGATTACTG CGGCGGATTG GGCCGGTGTT TCAACAAGCC CGGATCCTTC 
CAGTGCATCT GCCAGAAACC CTATTGCGGA GCCTACTGCA ACTTTACGGA TCCCTGCAAC 
GCTACGGACC TCTGCTCAAA CGGCGGTCGC TGCGTAGAGT CCTGCGGCGC CAAACCGGAC 
TACTACTGCG AGTGTCCGGA AGGTTTCGCG GGAAAGAATT GCACAGCACC GATTACGGCC 
AAGGAGGACG GGCCTTCGAC CACAGACATT GCCATCATTG TAATACCCGT AGTGGTGGTG 
CTGCTGCTGA TCGCGGGAGC CCTCCTGGGC ACCTTCCTGG TGATGGCCAG GAACAAGCGA 
GCAACCAGGG GCACCTATAG CCCGAGCGCG CAAGAGTACT GCAACCCACG GCTGGAAATG 
GACAACGTAC TGAAGCCACC GCCGGAAGAG CGACTAATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247284.1
CDS	550..7311
Translation	MAKIANASLSQQQKQRQAETATTTTTTVAASVETATTTARSRDRTKSAAQITSHLLKRAISVYSSPQWIPLFILIYLATDVASVAVPTKEAYFNGSTYLRLTTPMPIWDHSAISFRSCRGGEILAQQYNKNSIVISVLNDFLQISLAGPAVHGPNNRLDVKLPYQLLDNRWHTLQFKYEYGNLYLHVDRAASIFANSTYNSQFLTNQDIGYKDAILILGNSFSGCLLDGPGLQFVNNSTVQNVVFGHCPLTPGPCSDHDLFTRLPDNFCLNDPCMGHGTCSSSPEGYECRCTARYSGKNCQKDNGSPCAKNPCENGGSCLENSRGDYQCFCDPNHSGQHCETEVNIHPLCQTNPCLNNGACVVIGGSGALTCECPKGYAGARCEVDTDECASQPCQNNGSCIDRINGFSCDCSGTGYTGAFCQTNVDECDKNPCLNGGRCFDTYGWYTCQCLDGWGGEICDRPMTCQTQQCLNGGTCLDKPIGFQCLCPPEYTGELCQIAPSCAQQCPIDSECVGGKCVCKPGSSGPIGHCLPTTTTPTPEQEPTTTPRTTPNPNPAIPNTLTTTTKIPPITTSRTLVGTTTGSRRPPQQPLQSPTQRSASLNACPQENCLNGGTCLGYSGNYSCICASGYTGYNCQTSTGDGASALALTPINCNATNGKCLNGGTCSMNGTHCYCAVGYSGDRCEKAENCSPLNCQEPMVCVQNQCLCPENKVCNQCATQPCQNGGECVDLPNGDYECKCTRGWTGRTCGNDVDECTLHPKICGNGICKNEKGSYKCYCTPGFTGVHCDSDVDECLSFPCLNGATCHNKINAYECVCQPGYEGENCEVDIDECGSNPCSNGSTCIDRINNFTCNCIPGMTGRICDIDIDDCVGDPCLNGGQCIDQLGGFRCDCSGTGYEGENCELNIDECLSNPCTNGAKCLDRVKDYFCDCHNGYKGKNCEQDINECESNPCQYNGNCLERSNITLYQMSRITDLPKVFSQPFSFENASGYECVCVPGIIGKNCEININECDSNPCSKHGNCNDGIGTYTCECEPGFEGTHCEINIDECDRYNPCQRGTCYDQIDDYDCDCDANYGGKNCSVLLKGCDQNPCLNGGACLPYLINEVTHLYNCTCENGFQGDKCEKTTTLSMVATSLISVTTEREEGYDINLQFRTTLPNGVLAFGTTGEKNEPVSYILELINGRLNLHSSLLNKWEGVFIGSKLNDSNWHKVFVAINTSHLVLSANDEQAIFPVGSYETANNSQPSFPRTYLGGTIPNLKSYLRHLTHQPSAFVGCMQDIMVNGKWIFPDEQDANISYTKLENVQSGCPRTEQCKPNPCHSNGECTDLWHTFACHCPRPFFGHTCQHNMTAATFGHENTTHSAVIVETTDVARRAIRSILDISMFIRTREPTGQVFYLGTDPRKAPTKNIGDSYVAAKLHGGELLVKMQFSGTPEAYTVGGQKLDNGYNHLIEVVRNQTLVQVKLNGTEYFRKTLSTTGLLDAQVLYLGGPAPTRESLLGATTEPGIIPVPGAGIPIEDTTVPKEADDSRDYFKGIIQDVKVSNGSLNLIVEMYSLNVTDVQVNAKPLGAVTIDRASVLPGEVSDDLCRKNPCLHNAECRNTWNDYTCKCPNGYKGKNCQEIEFCQHVTCPGQSLCQNLDDGYECVTNTTFTGQERSPLAFFYFQEQQSDDIVSEASPKQTLKPVIDIAFRTRAGGTLLYIDNVDGFFEIGVNGGRVTITWKLSALHFGESARFEKENTDGEWSRIYLRAHNSKLEGGWKGWESMVDPTPAFSTDIDQAAFQSLIATSTQVYLGGMPESRQARGSTLSAQQGSQFKGCVGEARVGDLLLPYFSMAELYSRTNVSVQQKAQFRLNATRPEEGCILCFQSDCKNDGFCQSPSDEYACTCQPGFEGDDCGTDIDECLNTECLNNGTCINQVAAFFCQCQPGFEGQHCEQNIDECADQPCHNGGNCTDLIASYVCDCPEDYMGPQCDVLKQMTCENEPCRNGSTCQNGFNASTGNNFTCTCVPGFEGPLCDIPFCEITPCDNGGLCLTTGAVPMCKCSLGYTGRLCEQDINECESNPCQNGGQCKDLVGRYECDCQGTGFEGIRCENDIDECNMEGDYCGGLGRCFNKPGSFQCICQKPYCGAYCNFTDPCNATDLCSNGGRCVESCGAKPDYYCECPEGFAGKNCTAPITAKEDGPSTTDIAIIVIPVVVVLLLIAGALLGTFLVMARNKRATRGTYSPSAQEYCNPRLEMDNVLKPPPEERLI

Target ORF information:

RefSeq Version	NM_001260355.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster crumbs (crb), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260355.2

ORF Insert Sequence:

ATGGCTAAAA TCGCCAATGC GTCACTGTCG CAACAACAAA AACAGCGGCA AGCAGAAACA 
GCAACGACCA CAACAACAAC AGTGGCGGCA TCAGTAGAAA CAGCAACAAC AACAGCAAGA 
AGTCGAGACC GCACGAAAAG CGCAGCTCAA ATTACGTCGC ATTTGCTCAA GCGCGCCATT 
TCAGTTTATT CGTCGCCTCA ATGGATACCG CTCTTCATAC TTATTTACTT AGCAACAGAT 
GTCGCCTCAG TGGCGGTGCC GACGAAGGAG GCGTACTTTA ATGGCTCCAC TTACCTCCGC 
CTCACCACGC CGATGCCCAT TTGGGATCAC TCGGCGATTA GTTTCCGCTC GTGCCGCGGC 
GGCGAGATCC TCGCCCAGCA GTACAACAAG AACTCCATTG TAATCTCAGT GCTCAATGAC 
TTTCTGCAAA TCTCACTGGC TGGACCCGCC GTCCATGGGC CCAACAACCG GCTGGATGTC 
AAGCTGCCCT ACCAACTGCT GGACAACCGC TGGCATACGC TGCAGTTCAA GTACGAGTAC 
GGAAATCTCT ACCTGCATGT GGATCGCGCG GCAAGCATAT TTGCCAACTC CACGTACAAC 
AGTCAGTTCC TGACTAACCA GGACATTGGC TACAAGGACG CCATCTTGAT ACTGGGAAAC 
TCCTTCTCCG GTTGCCTTCT GGATGGACCA GGTCTGCAAT TTGTGAACAA CTCGACGGTG 
CAGAATGTGG TCTTTGGTCA CTGTCCCTTG ACCCCGGGTC CCTGCAGCGA TCACGATCTT 
TTCACTCGAC TGCCAGACAA CTTTTGTCTG AATGATCCCT GTATGGGCCA TGGAACCTGC 
TCGTCCAGTC CAGAGGGATA CGAATGTCGG TGCACGGCAC GTTACTCGGG AAAAAATTGC 
CAGAAGGACA ATGGCTCGCC GTGTGCCAAA AATCCATGTG AGAACGGTGG TTCCTGCCTG 
GAGAATTCCC GAGGGGATTA CCAGTGCTTC TGTGATCCCA ACCACAGTGG TCAGCACTGC 
GAGACGGAGG TGAACATCCA CCCACTCTGC CAGACGAACC CCTGCCTGAA CAATGGAGCA 
TGTGTGGTGA TTGGCGGCAG TGGAGCACTC ACCTGCGAGT GTCCCAAAGG ATACGCTGGT 
GCCAGGTGCG AGGTGGACAC GGATGAGTGC GCCTCACAGC CGTGTCAGAA CAACGGAAGC 
TGCATAGACC GTATCAATGG ATTCAGCTGC GATTGCAGCG GCACCGGTTA CACGGGTGCG 
TTCTGCCAGA CGAATGTGGA TGAGTGCGAC AAGAATCCGT GCCTGAACGG CGGCAGATGC 
TTCGATACCT ACGGATGGTA CACCTGCCAG TGTCTGGATG GTTGGGGCGG CGAGATTTGT 
GATCGACCCA TGACATGCCA GACGCAGCAG TGTTTGAACG GTGGCACCTG CCTGGACAAG 
CCCATCGGCT TCCAGTGCCT CTGTCCGCCG GAGTACACTG GTGAACTGTG CCAAATCGCA 
CCCAGTTGCG CACAGCAGTG CCCCATCGAT TCGGAGTGCG TAGGCGGCAA ATGCGTTTGT 
AAGCCAGGCT CATCGGGCCC CATTGGTCAC TGCCTGCCAA CAACAACCAC ACCGACACCA 
GAACAAGAGC CAACAACAAC GCCAAGAACC ACACCAAACC CTAACCCAGC TATTCCCAAC 
ACCCTAACAA CAACAACCAA GATACCACCA ATAACAACCA GCCGAACACT GGTGGGAACG 
ACCACGGGTA GCAGGAGACC ACCACAACAG CCACTACAAT CTCCGACCCA ACGCTCGGCC 
TCCCTGAATG CATGTCCCCA AGAAAACTGC TTGAACGGTG GCACCTGCCT GGGCTATAGT 
GGCAATTATT CCTGTATTTG TGCTAGTGGA TACACAGGTT ACAACTGTCA AACGAGCACG 
GGCGATGGAG CTTCTGCTTT GGCCCTGACA CCCATCAACT GCAATGCCAC CAATGGAAAG 
TGTCTCAATG GAGGAACTTG CTCCATGAAC GGAACCCACT GCTATTGCGC CGTCGGCTAT 
TCGGGAGATC GTTGCGAGAA GGCCGAGAAC TGTTCGCCAC TGAACTGCCA GGAACCGATG 
GTATGTGTCC AGAATCAGTG CCTCTGCCCG GAGAACAAGG TGTGCAACCA GTGCGCCACA 
CAACCGTGCC AGAATGGCGG TGAATGTGTG GACCTACCGA ATGGAGACTA CGAGTGCAAG 
TGCACCCGTG GATGGACTGG ACGCACCTGT GGCAACGACG TGGACGAGTG CACCCTGCAT 
CCGAAGATCT GCGGCAATGG TATCTGCAAG AACGAGAAGG GATCGTACAA ATGCTACTGT 
ACGCCTGGAT TTACCGGAGT CCACTGCGAT TCCGATGTGG ACGAATGCCT TAGCTTTCCC 
TGCCTCAACG GAGCCACGTG CCACAACAAG ATAAATGCCT ACGAGTGCGT TTGCCAACCA 
GGATATGAGG GAGAGAACTG CGAAGTAGAT ATCGATGAGT GCGGCAGTAA TCCCTGCTCG 
AACGGATCCA CCTGCATCGA CAGGATCAAC AACTTCACCT GCAACTGCAT TCCGGGAATG 
ACGGGCCGCA TATGTGACAT CGACATTGAC GACTGTGTGG GAGATCCCTG CTTGAATGGT 
GGTCAGTGCA TCGACCAATT GGGTGGCTTC CGTTGTGACT GCAGTGGCAC CGGTTATGAG 
GGCGAGAATT GTGAGCTGAA CATAGACGAA TGCCTCTCGA ATCCGTGCAC GAATGGTGCC 
AAGTGCCTGG ACAGGGTTAA GGACTACTTC TGCGATTGCC ACAACGGCTA CAAGGGAAAG 
AACTGTGAGC AGGACATCAA CGAGTGCGAG AGTAATCCCT GCCAGTACAA TGGCAACTGC 
CTAGAGCGTT CGAACATAAC GCTTTACCAA ATGAGTAGGA TCACGGATCT GCCCAAGGTG 
TTTAGCCAGC CCTTTAGCTT TGAGAATGCC AGCGGCTACG AGTGCGTCTG TGTGCCCGGC 
ATCATTGGAA AGAACTGCGA GATCAACATT AATGAGTGCG ACAGTAATCC ATGCAGCAAG 
CACGGAAATT GCAATGACGG GATTGGAACC TATACCTGCG AATGCGAACC TGGCTTCGAG 
GGCACCCACT GTGAGATCAA CATAGATGAA TGTGATCGCT ATAATCCCTG CCAGAGGGGC 
ACTTGCTATG ACCAGATAGA CGACTACGAC TGCGACTGCG ATGCGAACTA TGGAGGCAAA 
AACTGTTCCG TTCTTCTCAA AGGCTGTGAC CAGAATCCTT GTTTAAATGG CGGTGCCTGC 
TTGCCGTATT TGATCAACGA GGTAACTCAT CTGTACAACT GCACCTGCGA GAACGGCTTC 
CAGGGCGATA AATGCGAGAA GACCACCACT CTGTCCATGG TGGCCACTAG CCTGATTTCG 
GTTACCACGG AACGCGAGGA AGGCTACGAC ATTAATCTTC AATTTAGGAC CACACTGCCC 
AACGGAGTTT TGGCTTTCGG AACCACCGGC GAAAAGAATG AACCAGTTAG CTATATTTTG 
GAGCTGATTA ATGGACGACT GAATCTACAT TCCTCGCTCC TAAATAAGTG GGAGGGCGTG 
TTTATTGGAT CGAAGCTGAA CGATAGCAAC TGGCACAAGG TGTTTGTGGC CATAAACACT 
TCGCATCTAG TCCTTTCGGC CAACGACGAG CAGGCTATTT TTCCGGTTGG CTCCTATGAA 
ACGGCCAATA ATAGCCAGCC TTCATTCCCG CGCACATACC TCGGAGGCAC CATTCCCAAT 
CTGAAGTCCT ATCTGCGCCA CCTCACCCAC CAACCATCAG CTTTTGTGGG CTGCATGCAA 
GATATAATGG TCAATGGTAA ATGGATCTTT CCCGATGAGC AGGATGCGAA TATCAGTTAT 
ACCAAACTGG AAAATGTCCA GAGCGGTTGT CCGCGCACCG AACAATGTAA ACCGAATCCT 
TGCCATTCGA ACGGTGAATG CACGGACCTT TGGCACACCT TCGCTTGTCA CTGTCCCAGA 
CCATTCTTTG GGCACACATG TCAGCACAAT ATGACTGCTG CCACTTTTGG CCACGAGAAC 
ACAACCCATT CGGCCGTGAT TGTGGAGACC ACAGATGTGG CTAGGCGAGC TATTAGATCC 
ATCCTAGACA TTTCCATGTT TATTCGTACA CGCGAGCCAA CTGGTCAAGT CTTTTACTTG 
GGCACTGATC CACGCAAAGC CCCCACTAAA AATATTGGCG ACTCATATGT GGCCGCCAAA 
TTACACGGCG GCGAGCTGCT GGTAAAGATG CAGTTCAGCG GTACTCCGGA GGCCTACACC 
GTTGGTGGCC AGAAATTGGA CAACGGCTAT AACCACCTGA TCGAGGTGGT GCGCAATCAA 
ACGCTCGTGC AGGTCAAGCT CAATGGCACC GAGTACTTCC GCAAGACGCT GTCCACGACG 
GGTCTGCTGG ACGCACAGGT GCTTTACTTG GGCGGACCTG CACCCACACG CGAGTCACTT 
CTGGGCGCGA CCACAGAACC AGGAATAATT CCAGTTCCGG GAGCAGGAAT ACCCATTGAA 
GACACAACAG TGCCCAAGGA GGCGGACGAC AGCAGGGACT ACTTTAAGGG CATAATCCAG 
GACGTGAAGG TTAGCAATGG CTCGCTCAAC CTGATTGTGG AGATGTATTC CCTAAACGTG 
ACAGATGTCC AAGTGAATGC CAAACCTCTG GGCGCTGTAA CCATCGATCG TGCCTCCGTG 
TTACCTGGTG AGGTGTCCGA TGATCTGTGC CGAAAGAATC CCTGCCTCCA TAATGCCGAG 
TGCAGGAACA CATGGAATGA CTACACTTGC AAATGCCCAA ATGGTTACAA GGGAAAGAAC 
TGCCAGGAGA TCGAATTTTG CCAACACGTT ACATGTCCGG GACAGAGCTT GTGCCAAAAT 
CTGGACGATG GCTATGAGTG TGTTACGAAC ACCACATTTA CTGGGCAGGA ACGCAGTCCT 
CTCGCCTTCT TCTACTTCCA GGAGCAGCAA TCTGACGATA TTGTCAGTGA AGCGTCTCCC 
AAGCAAACTC TTAAACCGGT AATTGATATT GCCTTCCGCA CTCGTGCTGG AGGTACTCTC 
CTGTACATAG ACAATGTGGA CGGATTCTTT GAAATTGGAG TAAACGGAGG ACGAGTGACC 
ATCACCTGGA AGCTAAGTGC ACTGCATTTT GGCGAGTCTG CACGCTTTGA GAAGGAGAAC 
ACTGACGGAG AATGGAGTCG CATTTACCTC AGGGCACACA ACAGCAAACT GGAGGGTGGA 
TGGAAGGGAT GGGAATCGAT GGTGGATCCG ACGCCAGCTT TTTCCACGGA CATCGACCAA 
GCGGCCTTTC AGTCCCTGAT CGCTACAAGT ACCCAGGTTT ACTTGGGTGG CATGCCAGAG 
TCGCGACAAG CACGAGGATC CACTTTGTCT GCCCAGCAGG GCTCTCAGTT CAAGGGCTGT 
GTGGGAGAGG CAAGGGTGGG CGATCTTTTA CTGCCCTACT TCTCCATGGC GGAACTGTAT 
TCGCGCACCA ATGTTTCAGT ACAGCAAAAG GCTCAATTCC GTCTAAATGC CACACGACCT 
GAGGAGGGCT GCATCCTGTG CTTCCAGTCA GACTGCAAAA ATGACGGCTT CTGTCAATCT 
CCTTCAGATG AGTACGCCTG CACCTGTCAG CCTGGATTCG AGGGCGATGA TTGCGGCACG 
GACATCGACG AGTGTCTTAA CACGGAATGC TTGAACAACG GCACCTGCAT CAACCAGGTT 
GCAGCTTTCT TCTGCCAGTG TCAGCCAGGA TTCGAGGGTC AGCACTGTGA GCAAAACATC 
GACGAGTGTG CGGATCAGCC GTGCCACAAC GGTGGCAACT GCACGGATCT TATCGCATCG 
TACGTGTGCG ACTGCCCTGA GGACTATATG GGCCCGCAGT GCGACGTGCT GAAGCAAATG 
ACCTGCGAGA ACGAGCCATG TCGGAATGGA TCAACCTGCC AGAATGGATT CAATGCTTCT 
ACTGGCAATA ACTTTACATG TACATGCGTG CCCGGCTTCG AGGGTCCACT GTGTGACATA 
CCCTTCTGTG AAATAACGCC TTGCGATAAC GGTGGCCTCT GCCTGACCAC TGGAGCGGTA 
CCGATGTGCA AATGTAGTCT GGGATACACT GGTCGCCTGT GCGAGCAGGA CATTAACGAG 
TGCGAATCGA ATCCATGCCA GAACGGTGGT CAGTGTAAGG ACCTCGTCGG CAGGTACGAG 
TGCGATTGCC AGGGCACCGG GTTCGAGGGC ATTCGCTGTG AAAATGACAT CGACGAGTGC 
AACATGGAGG GAGATTACTG CGGCGGATTG GGCCGGTGTT TCAACAAGCC CGGATCCTTC 
CAGTGCATCT GCCAGAAACC CTATTGCGGA GCCTACTGCA ACTTTACGGA TCCCTGCAAC 
GCTACGGACC TCTGCTCAAA CGGCGGTCGC TGCGTAGAGT CCTGCGGCGC CAAACCGGAC 
TACTACTGCG AGTGTCCGGA AGGTTTCGCG GGAAAGAATT GCACAGCACC GATTACGGCC 
AAGGAGGACG GGCCTTCGAC CACAGACATT GCCATCATTG TAATACCCGT AGTGGTGGTG 
CTGCTGCTGA TCGCGGGAGC CCTCCTGGGC ACCTTCCTGG TGATGGCCAG GAACAAGCGA 
GCAACCAGGG GCACCTATAG CCCGAGCGCG CAAGAGTACT GCAACCCACG GCTGGAAATG 
GACAACGTAC TGAAGCCACC GCCGGAAGAG CGACTAATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crb ( NM_001275980.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262909.1 Drosophila melanogaster crumbs (crb), transcript variant D, mRNA.

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CloneID	OFa08722
Clone ID Related Accession (Same CDS sequence)	NM_001275980.1
Accession Version	NM_001275980.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	crumbs, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTAAAA TCGCCAATGC GTCACTGTCG CAACAACAAA AACAGCGGCA AGCAGAAACA 
GCAACGACCA CAACAACAAC AGTGGCGGCA TCAGTAGAAA CAGCAACAAC AACAGCAAGA 
AGTCGAGACC GCACGAAAAG CGCAGCTCAA ATTACGTCGC ATTTGCTCAA GCGCGCCATT 
TCAGTTTATT CGTCGCCTCA ATGGATACCG CTCTTCATAC TTATTTACTT AGCAACAGAT 
GTCGCCTCAG TGGCGGTGCC GACGAAGGAG GCGTACTTTA ATGGCTCCAC TTACCTCCGC 
CTCACCACGC CGATGCCCAT TTGGGATCAC TCGGCGATTA GTTTCCGCTC GTGCCGCGGC 
GGCGAGATCC TCGCCCAGCA GTACAACAAG AACTCCATTG TAATCTCAGT GCTCAATGAC 
TTTCTGCAAA TCTCACTGGC TGGACCCGCC GTCCATGGGC CCAACAACCG GCTGGATGTC 
AAGCTGCCCT ACCAACTGCT GGACAACCGC TGGCATACGC TGCAGTTCAA GTACGAGTAC 
GGAAATCTCT ACCTGCATGT GGATCGCGCG GCAAGCATAT TTGCCAACTC CACGTACAAC 
AGTCAGTTCC TGACTAACCA GGACATTGGC TACAAGGACG CCATCTTGAT ACTGGGAAAC 
TCCTTCTCCG GTTGCCTTCT GGATGGACCA GGTCTGCAAT TTGTGAACAA CTCGACGGTG 
CAGAATGTGG TCTTTGGTCA CTGTCCCTTG ACCCCGGGTC CCTGCAGCGA TCACGATCTT 
TTCACTCGAC TGCCAGACAA CTTTTGTCTG AATGATCCCT GTATGGGCCA TGGAACCTGC 
TCGTCCAGTC CAGAGGGATA CGAATGTCGG TGCACGGCAC GTTACTCGGG AAAAAATTGC 
CAGAAGGACA ATGGCTCGCC GTGTGCCAAA AATCCATGTG AGAACGGTGG TTCCTGCCTG 
GAGAATTCCC GAGGGGATTA CCAGTGCTTC TGTGATCCCA ACCACAGTGG TCAGCACTGC 
GAGACGGAGG TGAACATCCA CCCACTCTGC CAGACGAACC CCTGCCTGAA CAATGGAGCA 
TGTGTGGTGA TTGGCGGCAG TGGAGCACTC ACCTGCGAGT GTCCCAAAGG ATACGCTGGT 
GCCAGGTGCG AGGTGGACAC GGATGAGTGC GCCTCACAGC CGTGTCAGAA CAACGGAAGC 
TGCATAGACC GTATCAATGG ATTCAGCTGC GATTGCAGCG GCACCGGTTA CACGGGTGCG 
TTCTGCCAGA CGAATGTGGA TGAGTGCGAC AAGAATCCGT GCCTGAACGG CGGCAGATGC 
TTCGATACCT ACGGATGGTA CACCTGCCAG TGTCTGGATG GTTGGGGCGG CGAGATTTGT 
GATCGACCCA TGACATGCCA GACGCAGCAG TGTTTGAACG GTGGCACCTG CCTGGACAAG 
CCCATCGGCT TCCAGTGCCT CTGTCCGCCG GAGTACACTG GTGAACTGTG CCAAATCGCA 
CCCAGTTGCG CACAGCAGTG CCCCATCGAT TCGGAGTGCG TAGGCGGCAA ATGCGTTTGT 
AAGCCAGGCT CATCGGCTTC CGACATGGAG CCGCTGACTC CGCTCGAGCT GGACATTCTC 
GACGCCACGC TGTGCCCCAG CGAAAAGAAG AAGCGCTACA TATCGCCCGA GTGGCTCAAA 
CGCAAGCGTT GTGAGCTAAA ACTAAGTTAC AACTGTCAAA CGAGCACGGG CGATGGAGCT 
TCTGCTTTGG CCCTGACACC CATCAACTGC AATGCCACCA ATGGAAAGTG TCTCAATGGA 
GGAACTTGCT CCATGAACGG AACCCACTGC TATTGCGCCG TCGGCTATTC GGGAGATCGT 
TGCGAGAAGG CCGAGAACTG TTCGCCACTG AACTGCCAGG AACCGATGGT ATGTGTCCAG 
AATCAGTGCC TCTGCCCGGA GAACAAGGTG TGCAACCAGT GCGCCACACA ACCGTGCCAG 
AATGGCGGTG AATGTGTGGA CCTACCGAAT GGAGACTACG AGTGCAAGTG CACCCGTGGA 
TGGACTGGAC GCACCTGTGG CAACGACGTG GACGAGTGCA CCCTGCATCC GAAGATCTGC 
GGCAATGGTA TCTGCAAGAA CGAGAAGGGA TCGTACAAAT GCTACTGTAC GCCTGGATTT 
ACCGGAGTCC ACTGCGATTC CGATGTGGAC GAATGCCTTA GCTTTCCCTG CCTCAACGGA 
GCCACGTGCC ACAACAAGTT GTTTTTCTTT AAATTCGGAA AGCATCGGAT TTTGAGACAA 
ATAAATGCCT ACGAGTGCGT TTGCCAACCA GGATATGAGG GAGAGAACTG CGAAGTAGAT 
ATCGATGAGT GCGGCAGTAA TCCCTGCTCG AACGGATCCA CCTGCATCGA CAGGATCAAC 
AACTTCACCT GCAACTGCAT TCCGGGAATG ACGGGCCGCA TATGTGACAT CGACATTGAC 
GACTGTGTGG GAGATCCCTG CTTGAATGGT GGTCAGTGCA TCGACCAATT GGGTGGCTTC 
CGTTGTGACT GCAGTGGCAC CGGTTATGAG GGCGAGAATT GTGAGCTGAA CATAGACGAA 
TGCCTCTCGA ATCCGTGCAC GAATGGTGCC AAGTGCCTGG ACAGGGTTAA GGACTACTTC 
TGCGATTGCC ACAACGGCTA CAAGGGAAAG AACTGTGAGC AGGACATCAA CGAGTGCGAG 
AGTAATCCCT GCCAGTACAA TGGCAACTGC CTAGAGCGTT CGAACATAAC GCTTTACCAA 
ATGAGTAGGA TCACGGATCT GCCCAAGGTG TTTAGCCAGC CCTTTAGCTT TGAGAATGCC 
AGCGGCTACG AGTGCGTCTG TGTGCCCGGC ATCATTGGAA AGAACTGCGA GATCAACATT 
AATGAGTGCG ACAGTAATCC ATGCAGCAAG CACGGAAATT GCAATGACGG GATTGGAACC 
TATACCTGCG AATGCGAACC TGGCTTCGAG GGCACCCACT GTGAGATCAA CATAGATGAA 
TGTGATCGCT ATAATCCCTG CCAGAGGGGC ACTTGCTATG ACCAGATAGA CGACTACGAC 
TGCGACTGCG ATGCGAACTA TGGAGGCAAA AACTGTTCCG TTCTTCTCAA AGGCTGTGAC 
CAGAATCCTT GTTTAAATGG CGGTGCCTGC TTGCCGTATT TGATCAACGA GGTAACTCAT 
CTGTACAACT GCACCTGCGA GAACGGCTTC CAGGGCGATA AATGCGAGAA GACCACCACT 
CTGTCCATGG TGGCCACTAG CCTGATTTCG GTTACCACGG AACGCGAGGA AGGCTACGAC 
ATTAATCTTC AATTTAGGAC CACACTGCCC AACGGAGTTT TGGCTTTCGG AACCACCGGC 
GAAAAGAATG AACCAGTTAG CTATATTTTG GAGCTGATTA ATGGACGACT GAATCTACAT 
TCCTCGCTCC TAAATAAGTG GGAGGGCGTG TTTATTGGAT CGAAGCTGAA CGATAGCAAC 
TGGCACAAGG TGTTTGTGGC CATAAACACT TCGCATCTAG TCCTTTCGGC CAACGACGAG 
CAGGCTATTT TTCCGGTTGG CTCCTATGAA ACGGCCAATA ATAGCCAGCC TTCATTCCCG 
CGCACATACC TCGGAGGCAC CATTCCCAAT CTGAAGTCCT ATCTGCGCCA CCTCACCCAC 
CAACCATCAG CTTTTGTGGG CTGCATGCAA GATATAATGG TCAATGGTAA ATGGATCTTT 
CCCGATGAGC AGGATGCGAA TATCAGTTAT ACCAAACTGG AAAATGTCCA GAGCGGTTGT 
CCGCGCACCG AACAATGTAA ACCGAATCCT TGCCATTCGA ACGGTGAATG CACGGACCTT 
TGGCACACCT TCGCTTGTCA CTGTCCCAGA CCATTCTTTG GGCACACATG TCAGCACAAT 
ATGACTGCTG CCACTTTTGG CCACGAGAAC ACAACCCATT CGGCCGTGAT TGTGGAGACC 
ACAGATGTGG CTAGGCGAGC TATTAGATCC ATCCTAGACA TTTCCATGTT TATTCGTACA 
CGCGAGCCAA CTGGTCAAGT CTTTTACTTG GGCACTGATC CACGCAAAGC CCCCACTAAA 
AATATTGGCG ACTCATATGT GGCCGCCAAA TTACACGGCG GCGAGCTGCT GGTAAAGATG 
CAGTTCAGCG GTACTCCGGA GGCCTACACC GTTGGTGGCC AGAAATTGGA CAACGGCTAT 
AACCACCTGA TCGAGGTGGT GCGCAATCAA ACGCTCGTGC AGGTCAAGCT CAATGGCACC 
GAGTACTTCC GCAAGACGCT GTCCACGACG GGTCTGCTGG ACGCACAGGT GCTTTACTTG 
GGCGGACCTG CACCCACACG CGAGTCACTT CTGGGCGCGA CCACAGAACC AGGAATAATT 
CCAGTTCCGG GAGCAGGAAT ACCCATTGAA GACACAACAG TGCCCAAGGA GGCGGACGAC 
AGCAGGGACT ACTTTAAGGG CATAATCCAG GACGTGAAGG TTAGCAATGG CTCGCTCAAC 
CTGATTGTGG AGATGTATTC CCTAAACGTG ACAGATGTCC AAGTGAATGC CAAACCTCTG 
GGCGCTGTAA CCATCGATCG TGCCTCCGTG TTACCTGGTG AGGTGTCCGA TGATCTGTGC 
CGAAAGAATC CCTGCCTCCA TAATGCCGAG TGCAGGAACA CATGGAATGA CTACACTTGC 
AAATGCCCAA ATGGTTACAA GGGAAAGAAC TGCCAGGAGA TCGAATTTTG CCAACACGTT 
ACATGTCCGG GACAGAGCTT GTGCCAAAAT CTGGACGATG GCTATGAGTG TGTTACGAAC 
ACCACATTTA CTGGGCAGGA ACGCAGTCCT CTCGCCTTCT TCTACTTCCA GGAGCAGCAA 
TCTGACGATA TTGTCAGTGA AGCGTCTCCC AAGCAAACTC TTAAACCGGT AATTGATATT 
GCCTTCCGCA CTCGTGCTGG AGGTACTCTC CTGTACATAG ACAATGTGGA CGGATTCTTT 
GAAATTGGAG TAAACGGAGG ACGAGTGACC ATCACCTGGA AGCTAAGTGC ACTGCATTTT 
GGCGAGTCTG CACGCTTTGA GAAGGAGAAC ACTGACGGAG AATGGAGTCG CATTTACCTC 
AGGGCACACA ACAGCAAACT GGAGGGTGGA TGGAAGGGAT GGGAATCGAT GGTGGATCCG 
ACGCCAGCTT TTTCCACGGA CATCGACCAA GCGGCCTTTC AGTCCCTGAT CGCTACAAGT 
ACCCAGGTTT ACTTGGGTGG CATGCCAGAG TCGCGACAAG CACGAGGATC CACTTTGTCT 
GCCCAGCAGG GCTCTCAGTT CAAGGGCTGT GTGGGAGAGG CAAGGGTGGG CGATCTTTTA 
CTGCCCTACT TCTCCATGGC GGAACTGTAT TCGCGCACCA ATGTTTCAGT ACAGCAAAAG 
GCTCAATTCC GTCTAAATGC CACACGACCT GAGGAGGGCT GCATCCTGTG CTTCCAGTCA 
GACTGCAAAA ATGACGGCTT CTGTCAATCT CCTTCAGATG AGTACGCCTG CACCTGTCAG 
CCTGGATTCG AGGGCGATGA TTGCGGCACG GACATCGACG AGTGTCTTAA CACGGAATGC 
TTGAACAACG GCACCTGCAT CAACCAGGTT GCAGCTTTCT TCTGCCAGTG TCAGCCAGGA 
TTCGAGGGTC AGCACTGTGA GCAAAACATC GACGAGTGTG CGGATCAGCC GTGCCACAAC 
GGTGGCAACT GCACGGATCT TATCGCATCG TACGTGTGCG ACTGCCCTGA GGACTATATG 
GGCCCGCAGT GCGACGTGCT GAAGCAAATG ACCTGCGAGA ACGAGCCATG TCGGAATGGA 
TCAACCTGCC AGAATGGATT CAATGCTTCT ACTGGCAATA ACTTTACATG TACATGCGTG 
CCCGGCTTCG AGGGTCCACT GTGTGACATA CCCTTCTGTG AAATAACGCC TTGCGATAAC 
GGTGGCCTCT GCCTGACCAC TGGAGCGGTA CCGATGTGCA AATGTAGTCT GGGATACACT 
GGTCGCCTGT GCGAGCAGGA CATTAACGAG TGCGAATCGA ATCCATGCCA GAACGGTGGT 
CAGTGTAAGG ACCTCGTCGG CAGGTACGAG TGCGATTGCC AGGGCACCGG GTTCGAGGGC 
ATTCGCTGTG AAAATGACAT CGACGAGTGC AACATGGAGG GAGATTACTG CGGCGGATTG 
GGCCGGTGTT TCAACAAGCC CGGATCCTTC CAGTGCATCT GCCAGAAACC CTATTGCGGA 
GCCTACTGCA ACTTTACGGA TCCCTGCAAC GCTACGGACC TCTGCTCAAA CGGCGGTCGC 
TGCGTAGAGT CCTGCGGCGC CAAACCGGAC TACTACTGCG AGTGTCCGGA AGGTTTCGCG 
GGAAAGAATT GCACAGCACC GATTACGGCC AAGGAGGACG GGCCTTCGAC CACAGACATT 
GCCATCATTG TAATACCCGT AGTGGTGGTG CTGCTGCTGA TCGCGGGAGC CCTCCTGGGC 
ACCTTCCTGG TGATGGCCAG GAACAAGCGA GCAACCAGGG GCACCTATAG CCCGAGCGCG 
CAAGAGTACT GCAACCCACG GCTGGAAATG GACAACGTAC TGAAGCCACC GCCGGAAGAG 
CGACTAATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262909.1
CDS	550..7161
Translation	MAKIANASLSQQQKQRQAETATTTTTTVAASVETATTTARSRDRTKSAAQITSHLLKRAISVYSSPQWIPLFILIYLATDVASVAVPTKEAYFNGSTYLRLTTPMPIWDHSAISFRSCRGGEILAQQYNKNSIVISVLNDFLQISLAGPAVHGPNNRLDVKLPYQLLDNRWHTLQFKYEYGNLYLHVDRAASIFANSTYNSQFLTNQDIGYKDAILILGNSFSGCLLDGPGLQFVNNSTVQNVVFGHCPLTPGPCSDHDLFTRLPDNFCLNDPCMGHGTCSSSPEGYECRCTARYSGKNCQKDNGSPCAKNPCENGGSCLENSRGDYQCFCDPNHSGQHCETEVNIHPLCQTNPCLNNGACVVIGGSGALTCECPKGYAGARCEVDTDECASQPCQNNGSCIDRINGFSCDCSGTGYTGAFCQTNVDECDKNPCLNGGRCFDTYGWYTCQCLDGWGGEICDRPMTCQTQQCLNGGTCLDKPIGFQCLCPPEYTGELCQIAPSCAQQCPIDSECVGGKCVCKPGSSASDMEPLTPLELDILDATLCPSEKKKRYISPEWLKRKRCELKLSYNCQTSTGDGASALALTPINCNATNGKCLNGGTCSMNGTHCYCAVGYSGDRCEKAENCSPLNCQEPMVCVQNQCLCPENKVCNQCATQPCQNGGECVDLPNGDYECKCTRGWTGRTCGNDVDECTLHPKICGNGICKNEKGSYKCYCTPGFTGVHCDSDVDECLSFPCLNGATCHNKLFFFKFGKHRILRQINAYECVCQPGYEGENCEVDIDECGSNPCSNGSTCIDRINNFTCNCIPGMTGRICDIDIDDCVGDPCLNGGQCIDQLGGFRCDCSGTGYEGENCELNIDECLSNPCTNGAKCLDRVKDYFCDCHNGYKGKNCEQDINECESNPCQYNGNCLERSNITLYQMSRITDLPKVFSQPFSFENASGYECVCVPGIIGKNCEININECDSNPCSKHGNCNDGIGTYTCECEPGFEGTHCEINIDECDRYNPCQRGTCYDQIDDYDCDCDANYGGKNCSVLLKGCDQNPCLNGGACLPYLINEVTHLYNCTCENGFQGDKCEKTTTLSMVATSLISVTTEREEGYDINLQFRTTLPNGVLAFGTTGEKNEPVSYILELINGRLNLHSSLLNKWEGVFIGSKLNDSNWHKVFVAINTSHLVLSANDEQAIFPVGSYETANNSQPSFPRTYLGGTIPNLKSYLRHLTHQPSAFVGCMQDIMVNGKWIFPDEQDANISYTKLENVQSGCPRTEQCKPNPCHSNGECTDLWHTFACHCPRPFFGHTCQHNMTAATFGHENTTHSAVIVETTDVARRAIRSILDISMFIRTREPTGQVFYLGTDPRKAPTKNIGDSYVAAKLHGGELLVKMQFSGTPEAYTVGGQKLDNGYNHLIEVVRNQTLVQVKLNGTEYFRKTLSTTGLLDAQVLYLGGPAPTRESLLGATTEPGIIPVPGAGIPIEDTTVPKEADDSRDYFKGIIQDVKVSNGSLNLIVEMYSLNVTDVQVNAKPLGAVTIDRASVLPGEVSDDLCRKNPCLHNAECRNTWNDYTCKCPNGYKGKNCQEIEFCQHVTCPGQSLCQNLDDGYECVTNTTFTGQERSPLAFFYFQEQQSDDIVSEASPKQTLKPVIDIAFRTRAGGTLLYIDNVDGFFEIGVNGGRVTITWKLSALHFGESARFEKENTDGEWSRIYLRAHNSKLEGGWKGWESMVDPTPAFSTDIDQAAFQSLIATSTQVYLGGMPESRQARGSTLSAQQGSQFKGCVGEARVGDLLLPYFSMAELYSRTNVSVQQKAQFRLNATRPEEGCILCFQSDCKNDGFCQSPSDEYACTCQPGFEGDDCGTDIDECLNTECLNNGTCINQVAAFFCQCQPGFEGQHCEQNIDECADQPCHNGGNCTDLIASYVCDCPEDYMGPQCDVLKQMTCENEPCRNGSTCQNGFNASTGNNFTCTCVPGFEGPLCDIPFCEITPCDNGGLCLTTGAVPMCKCSLGYTGRLCEQDINECESNPCQNGGQCKDLVGRYECDCQGTGFEGIRCENDIDECNMEGDYCGGLGRCFNKPGSFQCICQKPYCGAYCNFTDPCNATDLCSNGGRCVESCGAKPDYYCECPEGFAGKNCTAPITAKEDGPSTTDIAIIVIPVVVVLLLIAGALLGTFLVMARNKRATRGTYSPSAQEYCNPRLEMDNVLKPPPEERLI

Target ORF information:

RefSeq Version	NM_001275980.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster crumbs (crb), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275980.1

ORF Insert Sequence:

ATGGCTAAAA TCGCCAATGC GTCACTGTCG CAACAACAAA AACAGCGGCA AGCAGAAACA 
GCAACGACCA CAACAACAAC AGTGGCGGCA TCAGTAGAAA CAGCAACAAC AACAGCAAGA 
AGTCGAGACC GCACGAAAAG CGCAGCTCAA ATTACGTCGC ATTTGCTCAA GCGCGCCATT 
TCAGTTTATT CGTCGCCTCA ATGGATACCG CTCTTCATAC TTATTTACTT AGCAACAGAT 
GTCGCCTCAG TGGCGGTGCC GACGAAGGAG GCGTACTTTA ATGGCTCCAC TTACCTCCGC 
CTCACCACGC CGATGCCCAT TTGGGATCAC TCGGCGATTA GTTTCCGCTC GTGCCGCGGC 
GGCGAGATCC TCGCCCAGCA GTACAACAAG AACTCCATTG TAATCTCAGT GCTCAATGAC 
TTTCTGCAAA TCTCACTGGC TGGACCCGCC GTCCATGGGC CCAACAACCG GCTGGATGTC 
AAGCTGCCCT ACCAACTGCT GGACAACCGC TGGCATACGC TGCAGTTCAA GTACGAGTAC 
GGAAATCTCT ACCTGCATGT GGATCGCGCG GCAAGCATAT TTGCCAACTC CACGTACAAC 
AGTCAGTTCC TGACTAACCA GGACATTGGC TACAAGGACG CCATCTTGAT ACTGGGAAAC 
TCCTTCTCCG GTTGCCTTCT GGATGGACCA GGTCTGCAAT TTGTGAACAA CTCGACGGTG 
CAGAATGTGG TCTTTGGTCA CTGTCCCTTG ACCCCGGGTC CCTGCAGCGA TCACGATCTT 
TTCACTCGAC TGCCAGACAA CTTTTGTCTG AATGATCCCT GTATGGGCCA TGGAACCTGC 
TCGTCCAGTC CAGAGGGATA CGAATGTCGG TGCACGGCAC GTTACTCGGG AAAAAATTGC 
CAGAAGGACA ATGGCTCGCC GTGTGCCAAA AATCCATGTG AGAACGGTGG TTCCTGCCTG 
GAGAATTCCC GAGGGGATTA CCAGTGCTTC TGTGATCCCA ACCACAGTGG TCAGCACTGC 
GAGACGGAGG TGAACATCCA CCCACTCTGC CAGACGAACC CCTGCCTGAA CAATGGAGCA 
TGTGTGGTGA TTGGCGGCAG TGGAGCACTC ACCTGCGAGT GTCCCAAAGG ATACGCTGGT 
GCCAGGTGCG AGGTGGACAC GGATGAGTGC GCCTCACAGC CGTGTCAGAA CAACGGAAGC 
TGCATAGACC GTATCAATGG ATTCAGCTGC GATTGCAGCG GCACCGGTTA CACGGGTGCG 
TTCTGCCAGA CGAATGTGGA TGAGTGCGAC AAGAATCCGT GCCTGAACGG CGGCAGATGC 
TTCGATACCT ACGGATGGTA CACCTGCCAG TGTCTGGATG GTTGGGGCGG CGAGATTTGT 
GATCGACCCA TGACATGCCA GACGCAGCAG TGTTTGAACG GTGGCACCTG CCTGGACAAG 
CCCATCGGCT TCCAGTGCCT CTGTCCGCCG GAGTACACTG GTGAACTGTG CCAAATCGCA 
CCCAGTTGCG CACAGCAGTG CCCCATCGAT TCGGAGTGCG TAGGCGGCAA ATGCGTTTGT 
AAGCCAGGCT CATCGGCTTC CGACATGGAG CCGCTGACTC CGCTCGAGCT GGACATTCTC 
GACGCCACGC TGTGCCCCAG CGAAAAGAAG AAGCGCTACA TATCGCCCGA GTGGCTCAAA 
CGCAAGCGTT GTGAGCTAAA ACTAAGTTAC AACTGTCAAA CGAGCACGGG CGATGGAGCT 
TCTGCTTTGG CCCTGACACC CATCAACTGC AATGCCACCA ATGGAAAGTG TCTCAATGGA 
GGAACTTGCT CCATGAACGG AACCCACTGC TATTGCGCCG TCGGCTATTC GGGAGATCGT 
TGCGAGAAGG CCGAGAACTG TTCGCCACTG AACTGCCAGG AACCGATGGT ATGTGTCCAG 
AATCAGTGCC TCTGCCCGGA GAACAAGGTG TGCAACCAGT GCGCCACACA ACCGTGCCAG 
AATGGCGGTG AATGTGTGGA CCTACCGAAT GGAGACTACG AGTGCAAGTG CACCCGTGGA 
TGGACTGGAC GCACCTGTGG CAACGACGTG GACGAGTGCA CCCTGCATCC GAAGATCTGC 
GGCAATGGTA TCTGCAAGAA CGAGAAGGGA TCGTACAAAT GCTACTGTAC GCCTGGATTT 
ACCGGAGTCC ACTGCGATTC CGATGTGGAC GAATGCCTTA GCTTTCCCTG CCTCAACGGA 
GCCACGTGCC ACAACAAGTT GTTTTTCTTT AAATTCGGAA AGCATCGGAT TTTGAGACAA 
ATAAATGCCT ACGAGTGCGT TTGCCAACCA GGATATGAGG GAGAGAACTG CGAAGTAGAT 
ATCGATGAGT GCGGCAGTAA TCCCTGCTCG AACGGATCCA CCTGCATCGA CAGGATCAAC 
AACTTCACCT GCAACTGCAT TCCGGGAATG ACGGGCCGCA TATGTGACAT CGACATTGAC 
GACTGTGTGG GAGATCCCTG CTTGAATGGT GGTCAGTGCA TCGACCAATT GGGTGGCTTC 
CGTTGTGACT GCAGTGGCAC CGGTTATGAG GGCGAGAATT GTGAGCTGAA CATAGACGAA 
TGCCTCTCGA ATCCGTGCAC GAATGGTGCC AAGTGCCTGG ACAGGGTTAA GGACTACTTC 
TGCGATTGCC ACAACGGCTA CAAGGGAAAG AACTGTGAGC AGGACATCAA CGAGTGCGAG 
AGTAATCCCT GCCAGTACAA TGGCAACTGC CTAGAGCGTT CGAACATAAC GCTTTACCAA 
ATGAGTAGGA TCACGGATCT GCCCAAGGTG TTTAGCCAGC CCTTTAGCTT TGAGAATGCC 
AGCGGCTACG AGTGCGTCTG TGTGCCCGGC ATCATTGGAA AGAACTGCGA GATCAACATT 
AATGAGTGCG ACAGTAATCC ATGCAGCAAG CACGGAAATT GCAATGACGG GATTGGAACC 
TATACCTGCG AATGCGAACC TGGCTTCGAG GGCACCCACT GTGAGATCAA CATAGATGAA 
TGTGATCGCT ATAATCCCTG CCAGAGGGGC ACTTGCTATG ACCAGATAGA CGACTACGAC 
TGCGACTGCG ATGCGAACTA TGGAGGCAAA AACTGTTCCG TTCTTCTCAA AGGCTGTGAC 
CAGAATCCTT GTTTAAATGG CGGTGCCTGC TTGCCGTATT TGATCAACGA GGTAACTCAT 
CTGTACAACT GCACCTGCGA GAACGGCTTC CAGGGCGATA AATGCGAGAA GACCACCACT 
CTGTCCATGG TGGCCACTAG CCTGATTTCG GTTACCACGG AACGCGAGGA AGGCTACGAC 
ATTAATCTTC AATTTAGGAC CACACTGCCC AACGGAGTTT TGGCTTTCGG AACCACCGGC 
GAAAAGAATG AACCAGTTAG CTATATTTTG GAGCTGATTA ATGGACGACT GAATCTACAT 
TCCTCGCTCC TAAATAAGTG GGAGGGCGTG TTTATTGGAT CGAAGCTGAA CGATAGCAAC 
TGGCACAAGG TGTTTGTGGC CATAAACACT TCGCATCTAG TCCTTTCGGC CAACGACGAG 
CAGGCTATTT TTCCGGTTGG CTCCTATGAA ACGGCCAATA ATAGCCAGCC TTCATTCCCG 
CGCACATACC TCGGAGGCAC CATTCCCAAT CTGAAGTCCT ATCTGCGCCA CCTCACCCAC 
CAACCATCAG CTTTTGTGGG CTGCATGCAA GATATAATGG TCAATGGTAA ATGGATCTTT 
CCCGATGAGC AGGATGCGAA TATCAGTTAT ACCAAACTGG AAAATGTCCA GAGCGGTTGT 
CCGCGCACCG AACAATGTAA ACCGAATCCT TGCCATTCGA ACGGTGAATG CACGGACCTT 
TGGCACACCT TCGCTTGTCA CTGTCCCAGA CCATTCTTTG GGCACACATG TCAGCACAAT 
ATGACTGCTG CCACTTTTGG CCACGAGAAC ACAACCCATT CGGCCGTGAT TGTGGAGACC 
ACAGATGTGG CTAGGCGAGC TATTAGATCC ATCCTAGACA TTTCCATGTT TATTCGTACA 
CGCGAGCCAA CTGGTCAAGT CTTTTACTTG GGCACTGATC CACGCAAAGC CCCCACTAAA 
AATATTGGCG ACTCATATGT GGCCGCCAAA TTACACGGCG GCGAGCTGCT GGTAAAGATG 
CAGTTCAGCG GTACTCCGGA GGCCTACACC GTTGGTGGCC AGAAATTGGA CAACGGCTAT 
AACCACCTGA TCGAGGTGGT GCGCAATCAA ACGCTCGTGC AGGTCAAGCT CAATGGCACC 
GAGTACTTCC GCAAGACGCT GTCCACGACG GGTCTGCTGG ACGCACAGGT GCTTTACTTG 
GGCGGACCTG CACCCACACG CGAGTCACTT CTGGGCGCGA CCACAGAACC AGGAATAATT 
CCAGTTCCGG GAGCAGGAAT ACCCATTGAA GACACAACAG TGCCCAAGGA GGCGGACGAC 
AGCAGGGACT ACTTTAAGGG CATAATCCAG GACGTGAAGG TTAGCAATGG CTCGCTCAAC 
CTGATTGTGG AGATGTATTC CCTAAACGTG ACAGATGTCC AAGTGAATGC CAAACCTCTG 
GGCGCTGTAA CCATCGATCG TGCCTCCGTG TTACCTGGTG AGGTGTCCGA TGATCTGTGC 
CGAAAGAATC CCTGCCTCCA TAATGCCGAG TGCAGGAACA CATGGAATGA CTACACTTGC 
AAATGCCCAA ATGGTTACAA GGGAAAGAAC TGCCAGGAGA TCGAATTTTG CCAACACGTT 
ACATGTCCGG GACAGAGCTT GTGCCAAAAT CTGGACGATG GCTATGAGTG TGTTACGAAC 
ACCACATTTA CTGGGCAGGA ACGCAGTCCT CTCGCCTTCT TCTACTTCCA GGAGCAGCAA 
TCTGACGATA TTGTCAGTGA AGCGTCTCCC AAGCAAACTC TTAAACCGGT AATTGATATT 
GCCTTCCGCA CTCGTGCTGG AGGTACTCTC CTGTACATAG ACAATGTGGA CGGATTCTTT 
GAAATTGGAG TAAACGGAGG ACGAGTGACC ATCACCTGGA AGCTAAGTGC ACTGCATTTT 
GGCGAGTCTG CACGCTTTGA GAAGGAGAAC ACTGACGGAG AATGGAGTCG CATTTACCTC 
AGGGCACACA ACAGCAAACT GGAGGGTGGA TGGAAGGGAT GGGAATCGAT GGTGGATCCG 
ACGCCAGCTT TTTCCACGGA CATCGACCAA GCGGCCTTTC AGTCCCTGAT CGCTACAAGT 
ACCCAGGTTT ACTTGGGTGG CATGCCAGAG TCGCGACAAG CACGAGGATC CACTTTGTCT 
GCCCAGCAGG GCTCTCAGTT CAAGGGCTGT GTGGGAGAGG CAAGGGTGGG CGATCTTTTA 
CTGCCCTACT TCTCCATGGC GGAACTGTAT TCGCGCACCA ATGTTTCAGT ACAGCAAAAG 
GCTCAATTCC GTCTAAATGC CACACGACCT GAGGAGGGCT GCATCCTGTG CTTCCAGTCA 
GACTGCAAAA ATGACGGCTT CTGTCAATCT CCTTCAGATG AGTACGCCTG CACCTGTCAG 
CCTGGATTCG AGGGCGATGA TTGCGGCACG GACATCGACG AGTGTCTTAA CACGGAATGC 
TTGAACAACG GCACCTGCAT CAACCAGGTT GCAGCTTTCT TCTGCCAGTG TCAGCCAGGA 
TTCGAGGGTC AGCACTGTGA GCAAAACATC GACGAGTGTG CGGATCAGCC GTGCCACAAC 
GGTGGCAACT GCACGGATCT TATCGCATCG TACGTGTGCG ACTGCCCTGA GGACTATATG 
GGCCCGCAGT GCGACGTGCT GAAGCAAATG ACCTGCGAGA ACGAGCCATG TCGGAATGGA 
TCAACCTGCC AGAATGGATT CAATGCTTCT ACTGGCAATA ACTTTACATG TACATGCGTG 
CCCGGCTTCG AGGGTCCACT GTGTGACATA CCCTTCTGTG AAATAACGCC TTGCGATAAC 
GGTGGCCTCT GCCTGACCAC TGGAGCGGTA CCGATGTGCA AATGTAGTCT GGGATACACT 
GGTCGCCTGT GCGAGCAGGA CATTAACGAG TGCGAATCGA ATCCATGCCA GAACGGTGGT 
CAGTGTAAGG ACCTCGTCGG CAGGTACGAG TGCGATTGCC AGGGCACCGG GTTCGAGGGC 
ATTCGCTGTG AAAATGACAT CGACGAGTGC AACATGGAGG GAGATTACTG CGGCGGATTG 
GGCCGGTGTT TCAACAAGCC CGGATCCTTC CAGTGCATCT GCCAGAAACC CTATTGCGGA 
GCCTACTGCA ACTTTACGGA TCCCTGCAAC GCTACGGACC TCTGCTCAAA CGGCGGTCGC 
TGCGTAGAGT CCTGCGGCGC CAAACCGGAC TACTACTGCG AGTGTCCGGA AGGTTTCGCG 
GGAAAGAATT GCACAGCACC GATTACGGCC AAGGAGGACG GGCCTTCGAC CACAGACATT 
GCCATCATTG TAATACCCGT AGTGGTGGTG CTGCTGCTGA TCGCGGGAGC CCTCCTGGGC 
ACCTTCCTGG TGATGGCCAG GAACAAGCGA GCAACCAGGG GCACCTATAG CCCGAGCGCG 
CAAGAGTACT GCAACCCACG GCTGGAAATG GACAACGTAC TGAAGCCACC GCCGGAAGAG 
CGACTAATTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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crb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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