CG10254 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG10254
Entrez Gene ID	42793
Full Name	CG10254 gene product from transcript CG10254-RB
Synonyms	BcDNA:LD22087,Dmel\CG10254
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 95A7-95A7

mRNA and Protein(s)

mRNA	Protein	Name
NM_170081.2	NP_732888.1	uncharacterized protein, isoform B
NM_001275949.1	NP_001262878.1	uncharacterized protein, isoform C
NM_142913.2	NP_651170.1	uncharacterized protein, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG10254	NP_001262878.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003090	XP_312777.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG10254 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG10254 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05814	NM_142913.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG10254), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa05814	NM_170081.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG10254), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa07996	NM_001275949.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG10254), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG10254 ( NM_170081.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732888.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG10254), mRNA.

Price & Availability↑

CloneID	OFa05814
Clone ID Related Accession (Same CDS sequence)	NM_170081.2 , NM_142913.2
Accession Version	NM_170081.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170081.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGCG ATGTGGCGGG TGCGGCCGCT CCAGGTGGTA CACCAGGTCA ACCGCAGCAG 
GAGCAGGAAC AGTCTTTGGC GGAGGCTGCT CCGCGTACAC CGACGCTTGC GAATCCTCCT 
CCATCCTCAC AGGAGCTGGG TCGGACCCCC AACCAGGAGA GCCGCCATAA CGCAAATTCC 
CACTCCCGTC GATCGGGTGG TGGCCAGGTG CCATCGAAAG CCCAGGGTAA CACCGAGTCC 
CAGTTCTTCT TAGAGGATGG GGTTTTTCGC ATTGATCGGC GCGGTCGCGT CCATTTTGGC 
TTGGTAACCG CAACGACCGA GACCTATTCC TCCTCCGAAG AGGAAGATGT CGATTCGCTG 
TCCAGGGGCG AGGTGCGTGT GGCCTTCTAT CCGGGTGGCA AGGAGATGGT GATGACGGAA 
CAGTCGATTG GTCTGGCGGA TCGCACCCTA ATGCCAGGCG ATGTGGTGCG GAGGCGTCTG 
CCAGGAGAAA ACGTTTTGGC TGGCCAGGCG GGCTACGTGC GGGATGTCAA TGTACGCGCC 
GATGTTATGG TAATGGGCTC CAGAATGGTC ATTAAAAATG TGCCCGCCGA GCGACTGCGT 
CCGATATCCG AGTGGAACCA ATATGTGCCT CTCTGCATGG ACACCTGGAT AGGCACCATC 
ATAGACATTA ATGAGTGCGC TGTGCTCAAA TCATCCAACG GTGCCCGTGT GGAAATCACC 
TCCAACGATT TCCACAAGTT TAAGGATGCT GTGTCCGAAT GCGATCGCAC GGTCTTCAAT 
CCGAATGTCT TCTTTCCAGG AAACATTGTA ATCGGTCGCA TGCCGCCCCC GGATCGTGTC 
AAGAATCTGA CCCCGGAGAT TACCCATCCC TTAACTCGCA AGGGTCGTGC TGTGTACACC 
GTGGAGTCTG TAGAAACTAC ATCAATTAAT GTGGATTGGT CCTGCCGTGC AATCGGAAAT 
GCCACGGGCA GCGATGACAC AGACAGCGAT CCTCTAAAGG AGCCAAGGTC CTTGATCAAG 
GGTGAGGAAC TGCACAAAGT CAAGCGCCTT AGCCTCTATG AGTCCTACGA GCTGCAAATT 
CACGATCGAT TCTACCTCAA ATATTCAAAA TGTGATCTAT TAGTTAAATA TGCCGACTGG 
GAAAATGAAC AAGCGGAGAA GTACATCCCC ATTTTTCACG CCCAGAAAAA AATGCAGCGC 
AAGGCCAGCA AATTGAACGC ATCTGCTTCC ACTAGTGGAA ATGCTCGCAG TGTACCAAAG 
TTCCGCCGCA TGCCCAATAA AATCAGCGGT ATGCGACTGC CTCGACCGCC AAGCGACCAG 
ACTCACAATG CTTGCAAGTC GTCTAAGCGC AGCAAAGAGG ATGTGTTGAC ATCGGAGGAT 
GAGGATGAGT TCGAGGAGTT CATGCAGCCA CTTGATCAAT CGATGACTGC CGAAAAAGAA 
GTGGAGGTTG CTGAGGACGC ACTGCCTCCG TCCGCTGCTA CCACAGCTGC AGCCGGTGTC 
GAAGTCCAGG CTCCCAGAAT AACGATGCGA TCTGCTAAGA GGCGACAAGT AAAGAAAAAA 
ATCCGCTCTG TCAAGAAAAC AATTTATTCG CCTTTTAATA AAAAGGAAAC CACTCCCAAG 
GATGGCGATG AAATCGTAGT GGAAACGCTG GTCGTTTACA GCTCGGTGAC GGTCGTCTGG 
CAGGATGGCA CGGTGGAGTC AGGCGTTCAC TCCACTCAGC TCTATCCTAT TCATCATTTG 
GACAACCATG AATTCTTTCC CGGTGATTTT GTGACCAAAG CCAACGATAT CAGTACGGGA 
GCCACAGACT ACGGGGTAAT TCAGTGCGTG GACCATGATG CACGCATAGC CAAGGTCATG 
TGGTTCAATA TCTACACGAA TATTGATAAT CCAATCCCTT TGTTCAAAGG ATTGGAGGAG 
CTGAGCGTTT ATGACATGAA GGATCACACC GATTACCAGT ACCGACCCGG CACAGTGGTG 
ATCCGAGTGT CAAACTTCAC GGGTGAGGAT GTTAACTCTA CTGCTGGTCA GGTGATCGAC 
AACTACCCGA ATGGTCGTGT GCGTGTTTGG TGGGCGAAGG GCCATATCAC ATTGTGCTAC 
CCTCAGGATC TCTTTCAACC ATTTGAACAA GATGAACAAA ATGAACAAGA TCAAGATGTC 
TACGGATCAG ATGGATCCGA AGACTCGTGG GAGACGGAAT CGGAAAACAG CCACATATCG 
TCATCGGACA ACGATGACGA CATTCTTACG GGCATTGACA GAGCCACAGA GGCAATGAAA 
GTGCTAGAAA AGATGTTCCG CGTTTTACAT CCCAATCGGA AAGCGGAAGT GCTTTACGAT 
CTGCTGGATG TGTACAAAAA TTGCCGGGCC TTAGACCGTT TGCTAAATAC TAAATTTTTC 
GACGAGTTTA ATTTCAAAGG CATTGTGCGT TGCAAGCCTA TTACCTCTAA AGTTTGTTCT 
GACAGTGCCA CCCATGAGCG CGCAACCCCA GAGACCCCAG AGACGTCGGC CGCCGTTCCC 
AAAGAGTCAT CAACGCTCCA AGCTGAGTTG GAACCAGTTT TAATGGATCC GTCTGTTCAG 
TCATCATCAA GTCCTACTAA GCGAAAATCC TCTACGGAGA TGGATGAAGT TCTATGCAAA 
AAGAGCACGG ATGAGATCTC GGAGGAGCAA GATTACCAGG TGGAGGTAGT AGAGGTAATA 
GAAGATCCGC CGCTAGTGAG GGAGTATCGC TGCAAGTACA ACGAAATGTT CGCCAACGCA 
CGTAGAAGCA TTGCAAAAGC GATTGAAAAT CGTACTAAGA ATGATTCTGG CGTACACTCT 
CGCGGTGAAA TCACCTCTGA CGAGTCTAAT AATCATCCAG ACAGCGAAAA TAAGTTGGAC 
ATAAAGCATC CCACAATAAA TGTAATGGAT GATTTGGAAA TTGAATTGGT TGCAAGTGGA 
GATGCTGACG TCTGCGAAGT CTTCTGTTAT TTGATCAAGT TTCAAATGGC CAGAATTCGC 
AAGACGGTCG CTGTGACGTT GGGCAAGACA AATGACAATC CCAAGCAAGA GAAAGAGGAT 
GTTAGCGATG CAGAGGAAGC TATTGAGGCG GTCAGCCAGG AAGCGGAACT AGCCAATGAT 
GGTGCTACCG AGTGCAATAC AATGGCTGAG GACGAAGCTA TGGAAGAGCC ATTGAATCTC 
AACGCGCTTT CCGAGTCACT GCCGGCTATT GACCAGACAG GTCCCATGGC CTGCTCCTCA 
CCATCTTGGT CAATAATTCC AGACACGCCC TCCGTGGACG AGAATTGTTT CCAAGTGCTG 
CCCAATGCAC CAGCTGCACA TAATTTTATT AGCAACCTGA TTACCCCGGC CAACAAGGCG 
CAGTATCAGC GAGCTGTGCA GCGGGAATAT CTTATGCTTA AGTCCTCGCT ACCTAACGGA 
GTTGTGGTAC GTGCCTACGA GGATCGCATG GACCTAATGT CTGTAATGAT GGTGGGTCCC 
AAGCGCACGC CCTACCAGAA CGCTCTATTC TTTTTCGACT TTCAATTTGG GCGCGATTAC 
CCGAAGAGCC CGCCGGTGTG CCACTACATC AGCTACTGCA CGGATCGTCT CAATCCAAAC 
TTGTACGAGG GTGGCAGGGT ATGTGTGTCG CTACTTGGCA CATGGATGGG ACGCGACAAC 
GAAGTTTGGT CGCCCAGCAG CACCATGCTC CAGGTGCTCG TCTCCATACA GGGACTCATA 
CTGGTCGATG AGCCCTACTA TAATGAGGCG GGCTACGAAA AGCAAAGAGG CACCCAACTG 
GGCAATGAGA ACTCTCGTGT CTACAACGAG ATGGCCATCA TAAAAATTGC CCAATCCACG 
GTGAAGCAGT TGACAAATCC GCCGCTCATC TTTCGCAATG AACTTATTGA GCACTTCAAG 
GAGTTTGGCA CCGAGCTGTA TGCCCGCATG CGGGCCTGGT CGGAGTATTC TCTTGAGGCA 
CAGCGCCAAA ATATAACCAA AATCAAGGAC ATGCCTGTGG AGTACAAGGC GTCTTGCGAG 
CTACCCGAAT TCCCGCTGCT ACCATGCAGT CGAGGCTTTT GCATAGCGGT CAAGCGCGTT 
TTGAGCCAAC TTCAAAGCGA GCTAACTGCC TTGGGTGCCA ATCTGGTTAC GGATGAACGT 
AATGGCATTG CGACTGGAAT TGGCACTGCC CCGCGCGCTG CTCCGGAGGA GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732888.1
CDS	109..4305
Translation	MMSDVAGAAAPGGTPGQPQQEQEQSLAEAAPRTPTLANPPPSSQELGRTPNQESRHNANSHSRRSGGGQVPSKAQGNTESQFFLEDGVFRIDRRGRVHFGLVTATTETYSSSEEEDVDSLSRGEVRVAFYPGGKEMVMTEQSIGLADRTLMPGDVVRRRLPGENVLAGQAGYVRDVNVRADVMVMGSRMVIKNVPAERLRPISEWNQYVPLCMDTWIGTIIDINECAVLKSSNGARVEITSNDFHKFKDAVSECDRTVFNPNVFFPGNIVIGRMPPPDRVKNLTPEITHPLTRKGRAVYTVESVETTSINVDWSCRAIGNATGSDDTDSDPLKEPRSLIKGEELHKVKRLSLYESYELQIHDRFYLKYSKCDLLVKYADWENEQAEKYIPIFHAQKKMQRKASKLNASASTSGNARSVPKFRRMPNKISGMRLPRPPSDQTHNACKSSKRSKEDVLTSEDEDEFEEFMQPLDQSMTAEKEVEVAEDALPPSAATTAAAGVEVQAPRITMRSAKRRQVKKKIRSVKKTIYSPFNKKETTPKDGDEIVVETLVVYSSVTVVWQDGTVESGVHSTQLYPIHHLDNHEFFPGDFVTKANDISTGATDYGVIQCVDHDARIAKVMWFNIYTNIDNPIPLFKGLEELSVYDMKDHTDYQYRPGTVVIRVSNFTGEDVNSTAGQVIDNYPNGRVRVWWAKGHITLCYPQDLFQPFEQDEQNEQDQDVYGSDGSEDSWETESENSHISSSDNDDDILTGIDRATEAMKVLEKMFRVLHPNRKAEVLYDLLDVYKNCRALDRLLNTKFFDEFNFKGIVRCKPITSKVCSDSATHERATPETPETSAAVPKESSTLQAELEPVLMDPSVQSSSSPTKRKSSTEMDEVLCKKSTDEISEEQDYQVEVVEVIEDPPLVREYRCKYNEMFANARRSIAKAIENRTKNDSGVHSRGEITSDESNNHPDSENKLDIKHPTINVMDDLEIELVASGDADVCEVFCYLIKFQMARIRKTVAVTLGKTNDNPKQEKEDVSDAEEAIEAVSQEAELANDGATECNTMAEDEAMEEPLNLNALSESLPAIDQTGPMACSSPSWSIIPDTPSVDENCFQVLPNAPAAHNFISNLITPANKAQYQRAVQREYLMLKSSLPNGVVVRAYEDRMDLMSVMMVGPKRTPYQNALFFFDFQFGRDYPKSPPVCHYISYCTDRLNPNLYEGGRVCVSLLGTWMGRDNEVWSPSSTMLQVLVSIQGLILVDEPYYNEAGYEKQRGTQLGNENSRVYNEMAIIKIAQSTVKQLTNPPLIFRNELIEHFKEFGTELYARMRAWSEYSLEAQRQNITKIKDMPVEYKASCELPEFPLLPCSRGFCIAVKRVLSQLQSELTALGANLVTDERNGIATGIGTAPRAAPEEI

Target ORF information:

RefSeq Version	NM_170081.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG10254), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170081.2

ORF Insert Sequence:

ATGATGAGCG ATGTGGCGGG TGCGGCCGCT CCAGGTGGTA CACCAGGTCA ACCGCAGCAG 
GAGCAGGAAC AGTCTTTGGC GGAGGCTGCT CCGCGTACAC CGACGCTTGC GAATCCTCCT 
CCATCCTCAC AGGAGCTGGG TCGGACCCCC AACCAGGAGA GCCGCCATAA CGCAAATTCC 
CACTCCCGTC GATCGGGTGG TGGCCAGGTG CCATCGAAAG CCCAGGGTAA CACCGAGTCC 
CAGTTCTTCT TAGAGGATGG GGTTTTTCGC ATTGATCGGC GCGGTCGCGT CCATTTTGGC 
TTGGTAACCG CAACGACCGA GACCTATTCC TCCTCCGAAG AGGAAGATGT CGATTCGCTG 
TCCAGGGGCG AGGTGCGTGT GGCCTTCTAT CCGGGTGGCA AGGAGATGGT GATGACGGAA 
CAGTCGATTG GTCTGGCGGA TCGCACCCTA ATGCCAGGCG ATGTGGTGCG GAGGCGTCTG 
CCAGGAGAAA ACGTTTTGGC TGGCCAGGCG GGCTACGTGC GGGATGTCAA TGTACGCGCC 
GATGTTATGG TAATGGGCTC CAGAATGGTC ATTAAAAATG TGCCCGCCGA GCGACTGCGT 
CCGATATCCG AGTGGAACCA ATATGTGCCT CTCTGCATGG ACACCTGGAT AGGCACCATC 
ATAGACATTA ATGAGTGCGC TGTGCTCAAA TCATCCAACG GTGCCCGTGT GGAAATCACC 
TCCAACGATT TCCACAAGTT TAAGGATGCT GTGTCCGAAT GCGATCGCAC GGTCTTCAAT 
CCGAATGTCT TCTTTCCAGG AAACATTGTA ATCGGTCGCA TGCCGCCCCC GGATCGTGTC 
AAGAATCTGA CCCCGGAGAT TACCCATCCC TTAACTCGCA AGGGTCGTGC TGTGTACACC 
GTGGAGTCTG TAGAAACTAC ATCAATTAAT GTGGATTGGT CCTGCCGTGC AATCGGAAAT 
GCCACGGGCA GCGATGACAC AGACAGCGAT CCTCTAAAGG AGCCAAGGTC CTTGATCAAG 
GGTGAGGAAC TGCACAAAGT CAAGCGCCTT AGCCTCTATG AGTCCTACGA GCTGCAAATT 
CACGATCGAT TCTACCTCAA ATATTCAAAA TGTGATCTAT TAGTTAAATA TGCCGACTGG 
GAAAATGAAC AAGCGGAGAA GTACATCCCC ATTTTTCACG CCCAGAAAAA AATGCAGCGC 
AAGGCCAGCA AATTGAACGC ATCTGCTTCC ACTAGTGGAA ATGCTCGCAG TGTACCAAAG 
TTCCGCCGCA TGCCCAATAA AATCAGCGGT ATGCGACTGC CTCGACCGCC AAGCGACCAG 
ACTCACAATG CTTGCAAGTC GTCTAAGCGC AGCAAAGAGG ATGTGTTGAC ATCGGAGGAT 
GAGGATGAGT TCGAGGAGTT CATGCAGCCA CTTGATCAAT CGATGACTGC CGAAAAAGAA 
GTGGAGGTTG CTGAGGACGC ACTGCCTCCG TCCGCTGCTA CCACAGCTGC AGCCGGTGTC 
GAAGTCCAGG CTCCCAGAAT AACGATGCGA TCTGCTAAGA GGCGACAAGT AAAGAAAAAA 
ATCCGCTCTG TCAAGAAAAC AATTTATTCG CCTTTTAATA AAAAGGAAAC CACTCCCAAG 
GATGGCGATG AAATCGTAGT GGAAACGCTG GTCGTTTACA GCTCGGTGAC GGTCGTCTGG 
CAGGATGGCA CGGTGGAGTC AGGCGTTCAC TCCACTCAGC TCTATCCTAT TCATCATTTG 
GACAACCATG AATTCTTTCC CGGTGATTTT GTGACCAAAG CCAACGATAT CAGTACGGGA 
GCCACAGACT ACGGGGTAAT TCAGTGCGTG GACCATGATG CACGCATAGC CAAGGTCATG 
TGGTTCAATA TCTACACGAA TATTGATAAT CCAATCCCTT TGTTCAAAGG ATTGGAGGAG 
CTGAGCGTTT ATGACATGAA GGATCACACC GATTACCAGT ACCGACCCGG CACAGTGGTG 
ATCCGAGTGT CAAACTTCAC GGGTGAGGAT GTTAACTCTA CTGCTGGTCA GGTGATCGAC 
AACTACCCGA ATGGTCGTGT GCGTGTTTGG TGGGCGAAGG GCCATATCAC ATTGTGCTAC 
CCTCAGGATC TCTTTCAACC ATTTGAACAA GATGAACAAA ATGAACAAGA TCAAGATGTC 
TACGGATCAG ATGGATCCGA AGACTCGTGG GAGACGGAAT CGGAAAACAG CCACATATCG 
TCATCGGACA ACGATGACGA CATTCTTACG GGCATTGACA GAGCCACAGA GGCAATGAAA 
GTGCTAGAAA AGATGTTCCG CGTTTTACAT CCCAATCGGA AAGCGGAAGT GCTTTACGAT 
CTGCTGGATG TGTACAAAAA TTGCCGGGCC TTAGACCGTT TGCTAAATAC TAAATTTTTC 
GACGAGTTTA ATTTCAAAGG CATTGTGCGT TGCAAGCCTA TTACCTCTAA AGTTTGTTCT 
GACAGTGCCA CCCATGAGCG CGCAACCCCA GAGACCCCAG AGACGTCGGC CGCCGTTCCC 
AAAGAGTCAT CAACGCTCCA AGCTGAGTTG GAACCAGTTT TAATGGATCC GTCTGTTCAG 
TCATCATCAA GTCCTACTAA GCGAAAATCC TCTACGGAGA TGGATGAAGT TCTATGCAAA 
AAGAGCACGG ATGAGATCTC GGAGGAGCAA GATTACCAGG TGGAGGTAGT AGAGGTAATA 
GAAGATCCGC CGCTAGTGAG GGAGTATCGC TGCAAGTACA ACGAAATGTT CGCCAACGCA 
CGTAGAAGCA TTGCAAAAGC GATTGAAAAT CGTACTAAGA ATGATTCTGG CGTACACTCT 
CGCGGTGAAA TCACCTCTGA CGAGTCTAAT AATCATCCAG ACAGCGAAAA TAAGTTGGAC 
ATAAAGCATC CCACAATAAA TGTAATGGAT GATTTGGAAA TTGAATTGGT TGCAAGTGGA 
GATGCTGACG TCTGCGAAGT CTTCTGTTAT TTGATCAAGT TTCAAATGGC CAGAATTCGC 
AAGACGGTCG CTGTGACGTT GGGCAAGACA AATGACAATC CCAAGCAAGA GAAAGAGGAT 
GTTAGCGATG CAGAGGAAGC TATTGAGGCG GTCAGCCAGG AAGCGGAACT AGCCAATGAT 
GGTGCTACCG AGTGCAATAC AATGGCTGAG GACGAAGCTA TGGAAGAGCC ATTGAATCTC 
AACGCGCTTT CCGAGTCACT GCCGGCTATT GACCAGACAG GTCCCATGGC CTGCTCCTCA 
CCATCTTGGT CAATAATTCC AGACACGCCC TCCGTGGACG AGAATTGTTT CCAAGTGCTG 
CCCAATGCAC CAGCTGCACA TAATTTTATT AGCAACCTGA TTACCCCGGC CAACAAGGCG 
CAGTATCAGC GAGCTGTGCA GCGGGAATAT CTTATGCTTA AGTCCTCGCT ACCTAACGGA 
GTTGTGGTAC GTGCCTACGA GGATCGCATG GACCTAATGT CTGTAATGAT GGTGGGTCCC 
AAGCGCACGC CCTACCAGAA CGCTCTATTC TTTTTCGACT TTCAATTTGG GCGCGATTAC 
CCGAAGAGCC CGCCGGTGTG CCACTACATC AGCTACTGCA CGGATCGTCT CAATCCAAAC 
TTGTACGAGG GTGGCAGGGT ATGTGTGTCG CTACTTGGCA CATGGATGGG ACGCGACAAC 
GAAGTTTGGT CGCCCAGCAG CACCATGCTC CAGGTGCTCG TCTCCATACA GGGACTCATA 
CTGGTCGATG AGCCCTACTA TAATGAGGCG GGCTACGAAA AGCAAAGAGG CACCCAACTG 
GGCAATGAGA ACTCTCGTGT CTACAACGAG ATGGCCATCA TAAAAATTGC CCAATCCACG 
GTGAAGCAGT TGACAAATCC GCCGCTCATC TTTCGCAATG AACTTATTGA GCACTTCAAG 
GAGTTTGGCA CCGAGCTGTA TGCCCGCATG CGGGCCTGGT CGGAGTATTC TCTTGAGGCA 
CAGCGCCAAA ATATAACCAA AATCAAGGAC ATGCCTGTGG AGTACAAGGC GTCTTGCGAG 
CTACCCGAAT TCCCGCTGCT ACCATGCAGT CGAGGCTTTT GCATAGCGGT CAAGCGCGTT 
TTGAGCCAAC TTCAAAGCGA GCTAACTGCC TTGGGTGCCA ATCTGGTTAC GGATGAACGT 
AATGGCATTG CGACTGGAAT TGGCACTGCC CCGCGCGCTG CTCCGGAGGA GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG10254 ( NM_142913.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651170.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG10254), mRNA.

Price & Availability↑

CloneID	OFa05814
Clone ID Related Accession (Same CDS sequence)	NM_170081.2 , NM_142913.2
Accession Version	NM_142913.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142913.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGCG ATGTGGCGGG TGCGGCCGCT CCAGGTGGTA CACCAGGTCA ACCGCAGCAG 
GAGCAGGAAC AGTCTTTGGC GGAGGCTGCT CCGCGTACAC CGACGCTTGC GAATCCTCCT 
CCATCCTCAC AGGAGCTGGG TCGGACCCCC AACCAGGAGA GCCGCCATAA CGCAAATTCC 
CACTCCCGTC GATCGGGTGG TGGCCAGGTG CCATCGAAAG CCCAGGGTAA CACCGAGTCC 
CAGTTCTTCT TAGAGGATGG GGTTTTTCGC ATTGATCGGC GCGGTCGCGT CCATTTTGGC 
TTGGTAACCG CAACGACCGA GACCTATTCC TCCTCCGAAG AGGAAGATGT CGATTCGCTG 
TCCAGGGGCG AGGTGCGTGT GGCCTTCTAT CCGGGTGGCA AGGAGATGGT GATGACGGAA 
CAGTCGATTG GTCTGGCGGA TCGCACCCTA ATGCCAGGCG ATGTGGTGCG GAGGCGTCTG 
CCAGGAGAAA ACGTTTTGGC TGGCCAGGCG GGCTACGTGC GGGATGTCAA TGTACGCGCC 
GATGTTATGG TAATGGGCTC CAGAATGGTC ATTAAAAATG TGCCCGCCGA GCGACTGCGT 
CCGATATCCG AGTGGAACCA ATATGTGCCT CTCTGCATGG ACACCTGGAT AGGCACCATC 
ATAGACATTA ATGAGTGCGC TGTGCTCAAA TCATCCAACG GTGCCCGTGT GGAAATCACC 
TCCAACGATT TCCACAAGTT TAAGGATGCT GTGTCCGAAT GCGATCGCAC GGTCTTCAAT 
CCGAATGTCT TCTTTCCAGG AAACATTGTA ATCGGTCGCA TGCCGCCCCC GGATCGTGTC 
AAGAATCTGA CCCCGGAGAT TACCCATCCC TTAACTCGCA AGGGTCGTGC TGTGTACACC 
GTGGAGTCTG TAGAAACTAC ATCAATTAAT GTGGATTGGT CCTGCCGTGC AATCGGAAAT 
GCCACGGGCA GCGATGACAC AGACAGCGAT CCTCTAAAGG AGCCAAGGTC CTTGATCAAG 
GGTGAGGAAC TGCACAAAGT CAAGCGCCTT AGCCTCTATG AGTCCTACGA GCTGCAAATT 
CACGATCGAT TCTACCTCAA ATATTCAAAA TGTGATCTAT TAGTTAAATA TGCCGACTGG 
GAAAATGAAC AAGCGGAGAA GTACATCCCC ATTTTTCACG CCCAGAAAAA AATGCAGCGC 
AAGGCCAGCA AATTGAACGC ATCTGCTTCC ACTAGTGGAA ATGCTCGCAG TGTACCAAAG 
TTCCGCCGCA TGCCCAATAA AATCAGCGGT ATGCGACTGC CTCGACCGCC AAGCGACCAG 
ACTCACAATG CTTGCAAGTC GTCTAAGCGC AGCAAAGAGG ATGTGTTGAC ATCGGAGGAT 
GAGGATGAGT TCGAGGAGTT CATGCAGCCA CTTGATCAAT CGATGACTGC CGAAAAAGAA 
GTGGAGGTTG CTGAGGACGC ACTGCCTCCG TCCGCTGCTA CCACAGCTGC AGCCGGTGTC 
GAAGTCCAGG CTCCCAGAAT AACGATGCGA TCTGCTAAGA GGCGACAAGT AAAGAAAAAA 
ATCCGCTCTG TCAAGAAAAC AATTTATTCG CCTTTTAATA AAAAGGAAAC CACTCCCAAG 
GATGGCGATG AAATCGTAGT GGAAACGCTG GTCGTTTACA GCTCGGTGAC GGTCGTCTGG 
CAGGATGGCA CGGTGGAGTC AGGCGTTCAC TCCACTCAGC TCTATCCTAT TCATCATTTG 
GACAACCATG AATTCTTTCC CGGTGATTTT GTGACCAAAG CCAACGATAT CAGTACGGGA 
GCCACAGACT ACGGGGTAAT TCAGTGCGTG GACCATGATG CACGCATAGC CAAGGTCATG 
TGGTTCAATA TCTACACGAA TATTGATAAT CCAATCCCTT TGTTCAAAGG ATTGGAGGAG 
CTGAGCGTTT ATGACATGAA GGATCACACC GATTACCAGT ACCGACCCGG CACAGTGGTG 
ATCCGAGTGT CAAACTTCAC GGGTGAGGAT GTTAACTCTA CTGCTGGTCA GGTGATCGAC 
AACTACCCGA ATGGTCGTGT GCGTGTTTGG TGGGCGAAGG GCCATATCAC ATTGTGCTAC 
CCTCAGGATC TCTTTCAACC ATTTGAACAA GATGAACAAA ATGAACAAGA TCAAGATGTC 
TACGGATCAG ATGGATCCGA AGACTCGTGG GAGACGGAAT CGGAAAACAG CCACATATCG 
TCATCGGACA ACGATGACGA CATTCTTACG GGCATTGACA GAGCCACAGA GGCAATGAAA 
GTGCTAGAAA AGATGTTCCG CGTTTTACAT CCCAATCGGA AAGCGGAAGT GCTTTACGAT 
CTGCTGGATG TGTACAAAAA TTGCCGGGCC TTAGACCGTT TGCTAAATAC TAAATTTTTC 
GACGAGTTTA ATTTCAAAGG CATTGTGCGT TGCAAGCCTA TTACCTCTAA AGTTTGTTCT 
GACAGTGCCA CCCATGAGCG CGCAACCCCA GAGACCCCAG AGACGTCGGC CGCCGTTCCC 
AAAGAGTCAT CAACGCTCCA AGCTGAGTTG GAACCAGTTT TAATGGATCC GTCTGTTCAG 
TCATCATCAA GTCCTACTAA GCGAAAATCC TCTACGGAGA TGGATGAAGT TCTATGCAAA 
AAGAGCACGG ATGAGATCTC GGAGGAGCAA GATTACCAGG TGGAGGTAGT AGAGGTAATA 
GAAGATCCGC CGCTAGTGAG GGAGTATCGC TGCAAGTACA ACGAAATGTT CGCCAACGCA 
CGTAGAAGCA TTGCAAAAGC GATTGAAAAT CGTACTAAGA ATGATTCTGG CGTACACTCT 
CGCGGTGAAA TCACCTCTGA CGAGTCTAAT AATCATCCAG ACAGCGAAAA TAAGTTGGAC 
ATAAAGCATC CCACAATAAA TGTAATGGAT GATTTGGAAA TTGAATTGGT TGCAAGTGGA 
GATGCTGACG TCTGCGAAGT CTTCTGTTAT TTGATCAAGT TTCAAATGGC CAGAATTCGC 
AAGACGGTCG CTGTGACGTT GGGCAAGACA AATGACAATC CCAAGCAAGA GAAAGAGGAT 
GTTAGCGATG CAGAGGAAGC TATTGAGGCG GTCAGCCAGG AAGCGGAACT AGCCAATGAT 
GGTGCTACCG AGTGCAATAC AATGGCTGAG GACGAAGCTA TGGAAGAGCC ATTGAATCTC 
AACGCGCTTT CCGAGTCACT GCCGGCTATT GACCAGACAG GTCCCATGGC CTGCTCCTCA 
CCATCTTGGT CAATAATTCC AGACACGCCC TCCGTGGACG AGAATTGTTT CCAAGTGCTG 
CCCAATGCAC CAGCTGCACA TAATTTTATT AGCAACCTGA TTACCCCGGC CAACAAGGCG 
CAGTATCAGC GAGCTGTGCA GCGGGAATAT CTTATGCTTA AGTCCTCGCT ACCTAACGGA 
GTTGTGGTAC GTGCCTACGA GGATCGCATG GACCTAATGT CTGTAATGAT GGTGGGTCCC 
AAGCGCACGC CCTACCAGAA CGCTCTATTC TTTTTCGACT TTCAATTTGG GCGCGATTAC 
CCGAAGAGCC CGCCGGTGTG CCACTACATC AGCTACTGCA CGGATCGTCT CAATCCAAAC 
TTGTACGAGG GTGGCAGGGT ATGTGTGTCG CTACTTGGCA CATGGATGGG ACGCGACAAC 
GAAGTTTGGT CGCCCAGCAG CACCATGCTC CAGGTGCTCG TCTCCATACA GGGACTCATA 
CTGGTCGATG AGCCCTACTA TAATGAGGCG GGCTACGAAA AGCAAAGAGG CACCCAACTG 
GGCAATGAGA ACTCTCGTGT CTACAACGAG ATGGCCATCA TAAAAATTGC CCAATCCACG 
GTGAAGCAGT TGACAAATCC GCCGCTCATC TTTCGCAATG AACTTATTGA GCACTTCAAG 
GAGTTTGGCA CCGAGCTGTA TGCCCGCATG CGGGCCTGGT CGGAGTATTC TCTTGAGGCA 
CAGCGCCAAA ATATAACCAA AATCAAGGAC ATGCCTGTGG AGTACAAGGC GTCTTGCGAG 
CTACCCGAAT TCCCGCTGCT ACCATGCAGT CGAGGCTTTT GCATAGCGGT CAAGCGCGTT 
TTGAGCCAAC TTCAAAGCGA GCTAACTGCC TTGGGTGCCA ATCTGGTTAC GGATGAACGT 
AATGGCATTG CGACTGGAAT TGGCACTGCC CCGCGCGCTG CTCCGGAGGA GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651170.1
CDS	279..4475
Translation	MMSDVAGAAAPGGTPGQPQQEQEQSLAEAAPRTPTLANPPPSSQELGRTPNQESRHNANSHSRRSGGGQVPSKAQGNTESQFFLEDGVFRIDRRGRVHFGLVTATTETYSSSEEEDVDSLSRGEVRVAFYPGGKEMVMTEQSIGLADRTLMPGDVVRRRLPGENVLAGQAGYVRDVNVRADVMVMGSRMVIKNVPAERLRPISEWNQYVPLCMDTWIGTIIDINECAVLKSSNGARVEITSNDFHKFKDAVSECDRTVFNPNVFFPGNIVIGRMPPPDRVKNLTPEITHPLTRKGRAVYTVESVETTSINVDWSCRAIGNATGSDDTDSDPLKEPRSLIKGEELHKVKRLSLYESYELQIHDRFYLKYSKCDLLVKYADWENEQAEKYIPIFHAQKKMQRKASKLNASASTSGNARSVPKFRRMPNKISGMRLPRPPSDQTHNACKSSKRSKEDVLTSEDEDEFEEFMQPLDQSMTAEKEVEVAEDALPPSAATTAAAGVEVQAPRITMRSAKRRQVKKKIRSVKKTIYSPFNKKETTPKDGDEIVVETLVVYSSVTVVWQDGTVESGVHSTQLYPIHHLDNHEFFPGDFVTKANDISTGATDYGVIQCVDHDARIAKVMWFNIYTNIDNPIPLFKGLEELSVYDMKDHTDYQYRPGTVVIRVSNFTGEDVNSTAGQVIDNYPNGRVRVWWAKGHITLCYPQDLFQPFEQDEQNEQDQDVYGSDGSEDSWETESENSHISSSDNDDDILTGIDRATEAMKVLEKMFRVLHPNRKAEVLYDLLDVYKNCRALDRLLNTKFFDEFNFKGIVRCKPITSKVCSDSATHERATPETPETSAAVPKESSTLQAELEPVLMDPSVQSSSSPTKRKSSTEMDEVLCKKSTDEISEEQDYQVEVVEVIEDPPLVREYRCKYNEMFANARRSIAKAIENRTKNDSGVHSRGEITSDESNNHPDSENKLDIKHPTINVMDDLEIELVASGDADVCEVFCYLIKFQMARIRKTVAVTLGKTNDNPKQEKEDVSDAEEAIEAVSQEAELANDGATECNTMAEDEAMEEPLNLNALSESLPAIDQTGPMACSSPSWSIIPDTPSVDENCFQVLPNAPAAHNFISNLITPANKAQYQRAVQREYLMLKSSLPNGVVVRAYEDRMDLMSVMMVGPKRTPYQNALFFFDFQFGRDYPKSPPVCHYISYCTDRLNPNLYEGGRVCVSLLGTWMGRDNEVWSPSSTMLQVLVSIQGLILVDEPYYNEAGYEKQRGTQLGNENSRVYNEMAIIKIAQSTVKQLTNPPLIFRNELIEHFKEFGTELYARMRAWSEYSLEAQRQNITKIKDMPVEYKASCELPEFPLLPCSRGFCIAVKRVLSQLQSELTALGANLVTDERNGIATGIGTAPRAAPEEI

Target ORF information:

RefSeq Version	NM_142913.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG10254), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142913.2

ORF Insert Sequence:

ATGATGAGCG ATGTGGCGGG TGCGGCCGCT CCAGGTGGTA CACCAGGTCA ACCGCAGCAG 
GAGCAGGAAC AGTCTTTGGC GGAGGCTGCT CCGCGTACAC CGACGCTTGC GAATCCTCCT 
CCATCCTCAC AGGAGCTGGG TCGGACCCCC AACCAGGAGA GCCGCCATAA CGCAAATTCC 
CACTCCCGTC GATCGGGTGG TGGCCAGGTG CCATCGAAAG CCCAGGGTAA CACCGAGTCC 
CAGTTCTTCT TAGAGGATGG GGTTTTTCGC ATTGATCGGC GCGGTCGCGT CCATTTTGGC 
TTGGTAACCG CAACGACCGA GACCTATTCC TCCTCCGAAG AGGAAGATGT CGATTCGCTG 
TCCAGGGGCG AGGTGCGTGT GGCCTTCTAT CCGGGTGGCA AGGAGATGGT GATGACGGAA 
CAGTCGATTG GTCTGGCGGA TCGCACCCTA ATGCCAGGCG ATGTGGTGCG GAGGCGTCTG 
CCAGGAGAAA ACGTTTTGGC TGGCCAGGCG GGCTACGTGC GGGATGTCAA TGTACGCGCC 
GATGTTATGG TAATGGGCTC CAGAATGGTC ATTAAAAATG TGCCCGCCGA GCGACTGCGT 
CCGATATCCG AGTGGAACCA ATATGTGCCT CTCTGCATGG ACACCTGGAT AGGCACCATC 
ATAGACATTA ATGAGTGCGC TGTGCTCAAA TCATCCAACG GTGCCCGTGT GGAAATCACC 
TCCAACGATT TCCACAAGTT TAAGGATGCT GTGTCCGAAT GCGATCGCAC GGTCTTCAAT 
CCGAATGTCT TCTTTCCAGG AAACATTGTA ATCGGTCGCA TGCCGCCCCC GGATCGTGTC 
AAGAATCTGA CCCCGGAGAT TACCCATCCC TTAACTCGCA AGGGTCGTGC TGTGTACACC 
GTGGAGTCTG TAGAAACTAC ATCAATTAAT GTGGATTGGT CCTGCCGTGC AATCGGAAAT 
GCCACGGGCA GCGATGACAC AGACAGCGAT CCTCTAAAGG AGCCAAGGTC CTTGATCAAG 
GGTGAGGAAC TGCACAAAGT CAAGCGCCTT AGCCTCTATG AGTCCTACGA GCTGCAAATT 
CACGATCGAT TCTACCTCAA ATATTCAAAA TGTGATCTAT TAGTTAAATA TGCCGACTGG 
GAAAATGAAC AAGCGGAGAA GTACATCCCC ATTTTTCACG CCCAGAAAAA AATGCAGCGC 
AAGGCCAGCA AATTGAACGC ATCTGCTTCC ACTAGTGGAA ATGCTCGCAG TGTACCAAAG 
TTCCGCCGCA TGCCCAATAA AATCAGCGGT ATGCGACTGC CTCGACCGCC AAGCGACCAG 
ACTCACAATG CTTGCAAGTC GTCTAAGCGC AGCAAAGAGG ATGTGTTGAC ATCGGAGGAT 
GAGGATGAGT TCGAGGAGTT CATGCAGCCA CTTGATCAAT CGATGACTGC CGAAAAAGAA 
GTGGAGGTTG CTGAGGACGC ACTGCCTCCG TCCGCTGCTA CCACAGCTGC AGCCGGTGTC 
GAAGTCCAGG CTCCCAGAAT AACGATGCGA TCTGCTAAGA GGCGACAAGT AAAGAAAAAA 
ATCCGCTCTG TCAAGAAAAC AATTTATTCG CCTTTTAATA AAAAGGAAAC CACTCCCAAG 
GATGGCGATG AAATCGTAGT GGAAACGCTG GTCGTTTACA GCTCGGTGAC GGTCGTCTGG 
CAGGATGGCA CGGTGGAGTC AGGCGTTCAC TCCACTCAGC TCTATCCTAT TCATCATTTG 
GACAACCATG AATTCTTTCC CGGTGATTTT GTGACCAAAG CCAACGATAT CAGTACGGGA 
GCCACAGACT ACGGGGTAAT TCAGTGCGTG GACCATGATG CACGCATAGC CAAGGTCATG 
TGGTTCAATA TCTACACGAA TATTGATAAT CCAATCCCTT TGTTCAAAGG ATTGGAGGAG 
CTGAGCGTTT ATGACATGAA GGATCACACC GATTACCAGT ACCGACCCGG CACAGTGGTG 
ATCCGAGTGT CAAACTTCAC GGGTGAGGAT GTTAACTCTA CTGCTGGTCA GGTGATCGAC 
AACTACCCGA ATGGTCGTGT GCGTGTTTGG TGGGCGAAGG GCCATATCAC ATTGTGCTAC 
CCTCAGGATC TCTTTCAACC ATTTGAACAA GATGAACAAA ATGAACAAGA TCAAGATGTC 
TACGGATCAG ATGGATCCGA AGACTCGTGG GAGACGGAAT CGGAAAACAG CCACATATCG 
TCATCGGACA ACGATGACGA CATTCTTACG GGCATTGACA GAGCCACAGA GGCAATGAAA 
GTGCTAGAAA AGATGTTCCG CGTTTTACAT CCCAATCGGA AAGCGGAAGT GCTTTACGAT 
CTGCTGGATG TGTACAAAAA TTGCCGGGCC TTAGACCGTT TGCTAAATAC TAAATTTTTC 
GACGAGTTTA ATTTCAAAGG CATTGTGCGT TGCAAGCCTA TTACCTCTAA AGTTTGTTCT 
GACAGTGCCA CCCATGAGCG CGCAACCCCA GAGACCCCAG AGACGTCGGC CGCCGTTCCC 
AAAGAGTCAT CAACGCTCCA AGCTGAGTTG GAACCAGTTT TAATGGATCC GTCTGTTCAG 
TCATCATCAA GTCCTACTAA GCGAAAATCC TCTACGGAGA TGGATGAAGT TCTATGCAAA 
AAGAGCACGG ATGAGATCTC GGAGGAGCAA GATTACCAGG TGGAGGTAGT AGAGGTAATA 
GAAGATCCGC CGCTAGTGAG GGAGTATCGC TGCAAGTACA ACGAAATGTT CGCCAACGCA 
CGTAGAAGCA TTGCAAAAGC GATTGAAAAT CGTACTAAGA ATGATTCTGG CGTACACTCT 
CGCGGTGAAA TCACCTCTGA CGAGTCTAAT AATCATCCAG ACAGCGAAAA TAAGTTGGAC 
ATAAAGCATC CCACAATAAA TGTAATGGAT GATTTGGAAA TTGAATTGGT TGCAAGTGGA 
GATGCTGACG TCTGCGAAGT CTTCTGTTAT TTGATCAAGT TTCAAATGGC CAGAATTCGC 
AAGACGGTCG CTGTGACGTT GGGCAAGACA AATGACAATC CCAAGCAAGA GAAAGAGGAT 
GTTAGCGATG CAGAGGAAGC TATTGAGGCG GTCAGCCAGG AAGCGGAACT AGCCAATGAT 
GGTGCTACCG AGTGCAATAC AATGGCTGAG GACGAAGCTA TGGAAGAGCC ATTGAATCTC 
AACGCGCTTT CCGAGTCACT GCCGGCTATT GACCAGACAG GTCCCATGGC CTGCTCCTCA 
CCATCTTGGT CAATAATTCC AGACACGCCC TCCGTGGACG AGAATTGTTT CCAAGTGCTG 
CCCAATGCAC CAGCTGCACA TAATTTTATT AGCAACCTGA TTACCCCGGC CAACAAGGCG 
CAGTATCAGC GAGCTGTGCA GCGGGAATAT CTTATGCTTA AGTCCTCGCT ACCTAACGGA 
GTTGTGGTAC GTGCCTACGA GGATCGCATG GACCTAATGT CTGTAATGAT GGTGGGTCCC 
AAGCGCACGC CCTACCAGAA CGCTCTATTC TTTTTCGACT TTCAATTTGG GCGCGATTAC 
CCGAAGAGCC CGCCGGTGTG CCACTACATC AGCTACTGCA CGGATCGTCT CAATCCAAAC 
TTGTACGAGG GTGGCAGGGT ATGTGTGTCG CTACTTGGCA CATGGATGGG ACGCGACAAC 
GAAGTTTGGT CGCCCAGCAG CACCATGCTC CAGGTGCTCG TCTCCATACA GGGACTCATA 
CTGGTCGATG AGCCCTACTA TAATGAGGCG GGCTACGAAA AGCAAAGAGG CACCCAACTG 
GGCAATGAGA ACTCTCGTGT CTACAACGAG ATGGCCATCA TAAAAATTGC CCAATCCACG 
GTGAAGCAGT TGACAAATCC GCCGCTCATC TTTCGCAATG AACTTATTGA GCACTTCAAG 
GAGTTTGGCA CCGAGCTGTA TGCCCGCATG CGGGCCTGGT CGGAGTATTC TCTTGAGGCA 
CAGCGCCAAA ATATAACCAA AATCAAGGAC ATGCCTGTGG AGTACAAGGC GTCTTGCGAG 
CTACCCGAAT TCCCGCTGCT ACCATGCAGT CGAGGCTTTT GCATAGCGGT CAAGCGCGTT 
TTGAGCCAAC TTCAAAGCGA GCTAACTGCC TTGGGTGCCA ATCTGGTTAC GGATGAACGT 
AATGGCATTG CGACTGGAAT TGGCACTGCC CCGCGCGCTG CTCCGGAGGA GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG10254 ( NM_001275949.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262878.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG10254), mRNA.

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CloneID	OFa07996
Clone ID Related Accession (Same CDS sequence)	NM_001275949.1
Accession Version	NM_001275949.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAGCG ATGTGGCGGG TGCGGCCGCT CCAGGTGGTA CACCAGGTCA ACCGCAGCAG 
GAGCAGGAAC AGTCTTTGGC GGAGGCTGCT CCGCGTACAC CGACGCTTGC GAATCCTCCT 
CCATCCTCAC AGGAGCTGGG TCGGACCCCC AACCAGGAGA GCCGCCATAA CGCAAATTCC 
CACTCCCGTC GATCGGGTGG TGGCCAGGTG CCATCGAAAG CCCAGGGTAA CACCGAGTCC 
CAGTTCTTCT TAGAGGATGG GGTTTTTCGC ATTGATCGGC GCGGTCGCGT CCATTTTGGC 
TTGGTAACCG CAACGACCGA GACCTATTCC TCCTCCGAAG AGGAAGATGT CGATTCGCTG 
TCCAGGGGCG AGGTGCGTGT GGCCTTCTAT CCGGGTGGCA AGGAGATGGT GATGACGGAA 
CAGTCGATTG GTCTGGCGGA TCGCACCCTA ATGCCAGGCG ATGTGGTGCG GAGGCGTCTG 
CCAGGAGAAA ACGTTTTGGC TGGCCAGGCG GGCTACGTGC GGGATGTCAA TGTACGCGCC 
GATGTTATGG TAATGGGCTC CAGAATGGTC ATTAAAAATG TGCCCGCCGA GCGACTGCGT 
CCGATATCCG AGTGGAACCA ATATGTGCCT CTCTGCATGG ACACCTGGAT AGGCACCATC 
ATAGACATTA ATGAGTGCGC TGTGCTCAAA TCATCCAACG GTGCCCGTGT GGAAATCACC 
TCCAACGATT TCCACAAGTT TAAGGATGCT GTGTCCGAAT GCGATCGCAC GGTCTTCAAT 
CCGAATGTCT TCTTTCCAGG AAACATTGTA ATCGGTCGCA TGCCGCCCCC GGATCGTGTC 
AAGAATCTGA CCCCGGAGAT TACCCATCCC TTAACTCGCA AGGGTCGTGC TGTGTACACC 
GTGGAGTCTG TAGAAACTAC ATCAATTAAT GTGGATTGGT CCTGCCGTGC AATCGGAAAT 
GCCACGGGCA GCGATGACAC AGACAGCGAT CCTCTAAAGG AGCCAAGGTC CTTGATCAAG 
GGTGAGGAAC TGCACAAAGT CAAGCGCCTT AGCCTCTATG AGTCCTACGA GCTGCAAATT 
CACGATCGAT TCTACCTCAA ATATTCAAAA TGTGATCTAT TAGTTAAATA TGCCGACTGG 
GAAAATGAAC AAGCGGAGAA GTACATCCCC ATTTTTCACG CCCAGAAAAA AATGCAGCGC 
AAGGCCAGCA AATTGAACGC ATCTGCTTCC ACTAGTGGAA ATGCTCGCAG TGTACCAAAG 
TTCCGCCGCA TGCCCAATAA AATCAGCGGT ATGCGACTGC CTCGACCGCC AAGCGACCAG 
ACTCACAATG CTTGCAAGTC GTCTAAGCGC AGCAAAGAGG ATGTGTTGAC ATCGGAGGAT 
GAGGATGAGT TCGAGGAGTT CATGCAGCCA CTTGATCAAT CGATGACTGC CGAAAAAGAA 
GTGGAGGTTG CTGAGGACGC ACTGCCTCCG TCCGCTGCTA CCACAGCTGC AGCCGGTGTC 
GAAGTCCAGG CTCCCAGAAT AACGATGCGA TCTGCTAAGA GGCGACAAGA AACCACTCCC 
AAGGATGGCG ATGAAATCGT AGTGGAAACG CTGGTCGTTT ACAGCTCGGT GACGGTCGTC 
TGGCAGGATG GCACGGTGGA GTCAGGCGTT CACTCCACTC AGCTCTATCC TATTCATCAT 
TTGGACAACC ATGAATTCTT TCCCGGTGAT TTTGTGACCA AAGCCAACGA TATCAGTACG 
GGAGCCACAG ACTACGGGGT AATTCAGTGC GTGGACCATG ATGCACGCAT AGCCAAGGTC 
ATGTGGTTCA ATATCTACAC GAATATTGAT AATCCAATCC CTTTGTTCAA AGGATTGGAG 
GAGCTGAGCG TTTATGACAT GAAGGATCAC ACCGATTACC AGTACCGACC CGGCACAGTG 
GTGATCCGAG TGTCAAACTT CACGGGTGAG GATGTTAACT CTACTGCTGG TCAGGTGATC 
GACAACTACC CGAATGGTCG TGTGCGTGTT TGGTGGGCGA AGGGCCATAT CACATTGTGC 
TACCCTCAGG ATCTCTTTCA ACCATTTGAA CAAGATGAAC AAAATGAACA AGATCAAGAT 
GTCTACGGAT CAGATGGATC CGAAGACTCG TGGGAGACGG AATCGGAAAA CAGCCACATA 
TCGTCATCGG ACAACGATGA CGACATTCTT ACGGGCATTG ACAGAGCCAC AGAGGCAATG 
AAAGTGCTAG AAAAGATGTT CCGCGTTTTA CATCCCAATC GGAAAGCGGA AGTGCTTTAC 
GATCTGCTGG ATGTGTACAA AAATTGCCGG GCCTTAGACC GTTTGCTAAA TACTAAATTT 
TTCGACGAGT TTAATTTCAA AGGCATTGTG CGTTGCAAGC CTATTACCTC TAAAGTTTGT 
TCTGACAGTG CCACCCATGA GCGCGCAACC CCAGAGACCC CAGAGACGTC GGCCGCCGTT 
CCCAAAGAGT CATCAACGCT CCAAGCTGAG TTGGAACCAG TTTTAATGGA TCCGTCTGTT 
CAGTCATCAT CAAGTCCTAC TAAGCGAAAA TCCTCTACGG AGATGGATGA AGTTCTATGC 
AAAAAGAGCA CGGATGAGAT CTCGGAGGAG CAAGATTACC AGGTGGAGGT AGTAGAGGTA 
ATAGAAGATC CGCCGCTAGT GAGGGAGTAT CGCTGCAAGT ACAACGAAAT GTTCGCCAAC 
GCACGTAGAA GCATTGCAAA AGCGATTGAA AATCGTACTA AGAATGATTC TGGCGTACAC 
TCTCGCGGTG AAATCACCTC TGACGAGTCT AATAATCATC CAGACAGCGA AAATAAGTTG 
GACATAAAGC ATCCCACAAT AAATGTAATG GATGATTTGG AAATTGAATT GGTTGCAAGT 
GGAGATGCTG ACGTCTGCGA AGTCTTCTGT TATTTGATCA AGTTTCAAAT GGCCAGAATT 
CGCAAGACGG TCGCTGTGAC GTTGGGCAAG ACAAATGACA ATCCCAAGCA AGAGAAAGAG 
GATGTTAGCG ATGCAGAGGA AGCTATTGAG GCGGTCAGCC AGGAAGCGGA ACTAGCCAAT 
GATGGTGCTA CCGAGTGCAA TACAATGGCT GAGGACGAAG CTATGGAAGA GCCATTGAAT 
CTCAACGCGC TTTCCGAGTC ACTGCCGGCT ATTGACCAGA CAGGTCCCAT GGCCTGCTCC 
TCACCATCTT GGTCAATAAT TCCAGACACG CCCTCCGTGG ACGAGAATTG TTTCCAAGTG 
CTGCCCAATG CACCAGCTGC ACATAATTTT ATTAGCAACC TGATTACCCC GGCCAACAAG 
GCGCAGTATC AGCGAGCTGT GCAGCGGGAA TATCTTATGC TTAAGTCCTC GCTACCTAAC 
GGAGTTGTGG TACGTGCCTA CGAGGATCGC ATGGACCTAA TGTCTGTAAT GATGGTGGGT 
CCCAAGCGCA CGCCCTACCA GAACGCTCTA TTCTTTTTCG ACTTTCAATT TGGGCGCGAT 
TACCCGAAGA GCCCGCCGGT GTGCCACTAC ATCAGCTACT GCACGGATCG TCTCAATCCA 
AACTTGTACG AGGGTGGCAG GGTATGTGTG TCGCTACTTG GCACATGGAT GGGACGCGAC 
AACGAAGTTT GGTCGCCCAG CAGCACCATG CTCCAGGTGC TCGTCTCCAT ACAGGGACTC 
ATACTGGTCG ATGAGCCCTA CTATAATGAG GCGGGCTACG AAAAGCAAAG AGGCACCCAA 
CTGGGCAATG AGAACTCTCG TGTCTACAAC GAGATGGCCA TCATAAAAAT TGCCCAATCC 
ACGGTGAAGC AGTTGACAAA TCCGCCGCTC ATCTTTCGCA ATGAACTTAT TGAGCACTTC 
AAGGAGTTTG GCACCGAGCT GTATGCCCGC ATGCGGGCCT GGTCGGAGTA TTCTCTTGAG 
GCACAGCGCC AAAATATAAC CAAAATCAAG GACATGCCTG TGGAGTACAA GGCGTCTTGC 
GAGCTACCCG AATTCCCGCT GCTACCATGC AGTCGAGGCT TTTGCATAGC GGTCAAGCGC 
GTTTTGAGCC AACTTCAAAG CGAGCTAACT GCCTTGGGTG CCAATCTGGT TACGGATGAA 
CGTAATGGCA TTGCGACTGG AATTGGCACT GCCCCGCGCG CTGCTCCGGA GGAGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262878.1
CDS	279..4418
Translation	MMSDVAGAAAPGGTPGQPQQEQEQSLAEAAPRTPTLANPPPSSQELGRTPNQESRHNANSHSRRSGGGQVPSKAQGNTESQFFLEDGVFRIDRRGRVHFGLVTATTETYSSSEEEDVDSLSRGEVRVAFYPGGKEMVMTEQSIGLADRTLMPGDVVRRRLPGENVLAGQAGYVRDVNVRADVMVMGSRMVIKNVPAERLRPISEWNQYVPLCMDTWIGTIIDINECAVLKSSNGARVEITSNDFHKFKDAVSECDRTVFNPNVFFPGNIVIGRMPPPDRVKNLTPEITHPLTRKGRAVYTVESVETTSINVDWSCRAIGNATGSDDTDSDPLKEPRSLIKGEELHKVKRLSLYESYELQIHDRFYLKYSKCDLLVKYADWENEQAEKYIPIFHAQKKMQRKASKLNASASTSGNARSVPKFRRMPNKISGMRLPRPPSDQTHNACKSSKRSKEDVLTSEDEDEFEEFMQPLDQSMTAEKEVEVAEDALPPSAATTAAAGVEVQAPRITMRSAKRRQETTPKDGDEIVVETLVVYSSVTVVWQDGTVESGVHSTQLYPIHHLDNHEFFPGDFVTKANDISTGATDYGVIQCVDHDARIAKVMWFNIYTNIDNPIPLFKGLEELSVYDMKDHTDYQYRPGTVVIRVSNFTGEDVNSTAGQVIDNYPNGRVRVWWAKGHITLCYPQDLFQPFEQDEQNEQDQDVYGSDGSEDSWETESENSHISSSDNDDDILTGIDRATEAMKVLEKMFRVLHPNRKAEVLYDLLDVYKNCRALDRLLNTKFFDEFNFKGIVRCKPITSKVCSDSATHERATPETPETSAAVPKESSTLQAELEPVLMDPSVQSSSSPTKRKSSTEMDEVLCKKSTDEISEEQDYQVEVVEVIEDPPLVREYRCKYNEMFANARRSIAKAIENRTKNDSGVHSRGEITSDESNNHPDSENKLDIKHPTINVMDDLEIELVASGDADVCEVFCYLIKFQMARIRKTVAVTLGKTNDNPKQEKEDVSDAEEAIEAVSQEAELANDGATECNTMAEDEAMEEPLNLNALSESLPAIDQTGPMACSSPSWSIIPDTPSVDENCFQVLPNAPAAHNFISNLITPANKAQYQRAVQREYLMLKSSLPNGVVVRAYEDRMDLMSVMMVGPKRTPYQNALFFFDFQFGRDYPKSPPVCHYISYCTDRLNPNLYEGGRVCVSLLGTWMGRDNEVWSPSSTMLQVLVSIQGLILVDEPYYNEAGYEKQRGTQLGNENSRVYNEMAIIKIAQSTVKQLTNPPLIFRNELIEHFKEFGTELYARMRAWSEYSLEAQRQNITKIKDMPVEYKASCELPEFPLLPCSRGFCIAVKRVLSQLQSELTALGANLVTDERNGIATGIGTAPRAAPEEI

Target ORF information:

RefSeq Version	NM_001275949.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG10254), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275949.1

ORF Insert Sequence:

ATGATGAGCG ATGTGGCGGG TGCGGCCGCT CCAGGTGGTA CACCAGGTCA ACCGCAGCAG 
GAGCAGGAAC AGTCTTTGGC GGAGGCTGCT CCGCGTACAC CGACGCTTGC GAATCCTCCT 
CCATCCTCAC AGGAGCTGGG TCGGACCCCC AACCAGGAGA GCCGCCATAA CGCAAATTCC 
CACTCCCGTC GATCGGGTGG TGGCCAGGTG CCATCGAAAG CCCAGGGTAA CACCGAGTCC 
CAGTTCTTCT TAGAGGATGG GGTTTTTCGC ATTGATCGGC GCGGTCGCGT CCATTTTGGC 
TTGGTAACCG CAACGACCGA GACCTATTCC TCCTCCGAAG AGGAAGATGT CGATTCGCTG 
TCCAGGGGCG AGGTGCGTGT GGCCTTCTAT CCGGGTGGCA AGGAGATGGT GATGACGGAA 
CAGTCGATTG GTCTGGCGGA TCGCACCCTA ATGCCAGGCG ATGTGGTGCG GAGGCGTCTG 
CCAGGAGAAA ACGTTTTGGC TGGCCAGGCG GGCTACGTGC GGGATGTCAA TGTACGCGCC 
GATGTTATGG TAATGGGCTC CAGAATGGTC ATTAAAAATG TGCCCGCCGA GCGACTGCGT 
CCGATATCCG AGTGGAACCA ATATGTGCCT CTCTGCATGG ACACCTGGAT AGGCACCATC 
ATAGACATTA ATGAGTGCGC TGTGCTCAAA TCATCCAACG GTGCCCGTGT GGAAATCACC 
TCCAACGATT TCCACAAGTT TAAGGATGCT GTGTCCGAAT GCGATCGCAC GGTCTTCAAT 
CCGAATGTCT TCTTTCCAGG AAACATTGTA ATCGGTCGCA TGCCGCCCCC GGATCGTGTC 
AAGAATCTGA CCCCGGAGAT TACCCATCCC TTAACTCGCA AGGGTCGTGC TGTGTACACC 
GTGGAGTCTG TAGAAACTAC ATCAATTAAT GTGGATTGGT CCTGCCGTGC AATCGGAAAT 
GCCACGGGCA GCGATGACAC AGACAGCGAT CCTCTAAAGG AGCCAAGGTC CTTGATCAAG 
GGTGAGGAAC TGCACAAAGT CAAGCGCCTT AGCCTCTATG AGTCCTACGA GCTGCAAATT 
CACGATCGAT TCTACCTCAA ATATTCAAAA TGTGATCTAT TAGTTAAATA TGCCGACTGG 
GAAAATGAAC AAGCGGAGAA GTACATCCCC ATTTTTCACG CCCAGAAAAA AATGCAGCGC 
AAGGCCAGCA AATTGAACGC ATCTGCTTCC ACTAGTGGAA ATGCTCGCAG TGTACCAAAG 
TTCCGCCGCA TGCCCAATAA AATCAGCGGT ATGCGACTGC CTCGACCGCC AAGCGACCAG 
ACTCACAATG CTTGCAAGTC GTCTAAGCGC AGCAAAGAGG ATGTGTTGAC ATCGGAGGAT 
GAGGATGAGT TCGAGGAGTT CATGCAGCCA CTTGATCAAT CGATGACTGC CGAAAAAGAA 
GTGGAGGTTG CTGAGGACGC ACTGCCTCCG TCCGCTGCTA CCACAGCTGC AGCCGGTGTC 
GAAGTCCAGG CTCCCAGAAT AACGATGCGA TCTGCTAAGA GGCGACAAGA AACCACTCCC 
AAGGATGGCG ATGAAATCGT AGTGGAAACG CTGGTCGTTT ACAGCTCGGT GACGGTCGTC 
TGGCAGGATG GCACGGTGGA GTCAGGCGTT CACTCCACTC AGCTCTATCC TATTCATCAT 
TTGGACAACC ATGAATTCTT TCCCGGTGAT TTTGTGACCA AAGCCAACGA TATCAGTACG 
GGAGCCACAG ACTACGGGGT AATTCAGTGC GTGGACCATG ATGCACGCAT AGCCAAGGTC 
ATGTGGTTCA ATATCTACAC GAATATTGAT AATCCAATCC CTTTGTTCAA AGGATTGGAG 
GAGCTGAGCG TTTATGACAT GAAGGATCAC ACCGATTACC AGTACCGACC CGGCACAGTG 
GTGATCCGAG TGTCAAACTT CACGGGTGAG GATGTTAACT CTACTGCTGG TCAGGTGATC 
GACAACTACC CGAATGGTCG TGTGCGTGTT TGGTGGGCGA AGGGCCATAT CACATTGTGC 
TACCCTCAGG ATCTCTTTCA ACCATTTGAA CAAGATGAAC AAAATGAACA AGATCAAGAT 
GTCTACGGAT CAGATGGATC CGAAGACTCG TGGGAGACGG AATCGGAAAA CAGCCACATA 
TCGTCATCGG ACAACGATGA CGACATTCTT ACGGGCATTG ACAGAGCCAC AGAGGCAATG 
AAAGTGCTAG AAAAGATGTT CCGCGTTTTA CATCCCAATC GGAAAGCGGA AGTGCTTTAC 
GATCTGCTGG ATGTGTACAA AAATTGCCGG GCCTTAGACC GTTTGCTAAA TACTAAATTT 
TTCGACGAGT TTAATTTCAA AGGCATTGTG CGTTGCAAGC CTATTACCTC TAAAGTTTGT 
TCTGACAGTG CCACCCATGA GCGCGCAACC CCAGAGACCC CAGAGACGTC GGCCGCCGTT 
CCCAAAGAGT CATCAACGCT CCAAGCTGAG TTGGAACCAG TTTTAATGGA TCCGTCTGTT 
CAGTCATCAT CAAGTCCTAC TAAGCGAAAA TCCTCTACGG AGATGGATGA AGTTCTATGC 
AAAAAGAGCA CGGATGAGAT CTCGGAGGAG CAAGATTACC AGGTGGAGGT AGTAGAGGTA 
ATAGAAGATC CGCCGCTAGT GAGGGAGTAT CGCTGCAAGT ACAACGAAAT GTTCGCCAAC 
GCACGTAGAA GCATTGCAAA AGCGATTGAA AATCGTACTA AGAATGATTC TGGCGTACAC 
TCTCGCGGTG AAATCACCTC TGACGAGTCT AATAATCATC CAGACAGCGA AAATAAGTTG 
GACATAAAGC ATCCCACAAT AAATGTAATG GATGATTTGG AAATTGAATT GGTTGCAAGT 
GGAGATGCTG ACGTCTGCGA AGTCTTCTGT TATTTGATCA AGTTTCAAAT GGCCAGAATT 
CGCAAGACGG TCGCTGTGAC GTTGGGCAAG ACAAATGACA ATCCCAAGCA AGAGAAAGAG 
GATGTTAGCG ATGCAGAGGA AGCTATTGAG GCGGTCAGCC AGGAAGCGGA ACTAGCCAAT 
GATGGTGCTA CCGAGTGCAA TACAATGGCT GAGGACGAAG CTATGGAAGA GCCATTGAAT 
CTCAACGCGC TTTCCGAGTC ACTGCCGGCT ATTGACCAGA CAGGTCCCAT GGCCTGCTCC 
TCACCATCTT GGTCAATAAT TCCAGACACG CCCTCCGTGG ACGAGAATTG TTTCCAAGTG 
CTGCCCAATG CACCAGCTGC ACATAATTTT ATTAGCAACC TGATTACCCC GGCCAACAAG 
GCGCAGTATC AGCGAGCTGT GCAGCGGGAA TATCTTATGC TTAAGTCCTC GCTACCTAAC 
GGAGTTGTGG TACGTGCCTA CGAGGATCGC ATGGACCTAA TGTCTGTAAT GATGGTGGGT 
CCCAAGCGCA CGCCCTACCA GAACGCTCTA TTCTTTTTCG ACTTTCAATT TGGGCGCGAT 
TACCCGAAGA GCCCGCCGGT GTGCCACTAC ATCAGCTACT GCACGGATCG TCTCAATCCA 
AACTTGTACG AGGGTGGCAG GGTATGTGTG TCGCTACTTG GCACATGGAT GGGACGCGAC 
AACGAAGTTT GGTCGCCCAG CAGCACCATG CTCCAGGTGC TCGTCTCCAT ACAGGGACTC 
ATACTGGTCG ATGAGCCCTA CTATAATGAG GCGGGCTACG AAAAGCAAAG AGGCACCCAA 
CTGGGCAATG AGAACTCTCG TGTCTACAAC GAGATGGCCA TCATAAAAAT TGCCCAATCC 
ACGGTGAAGC AGTTGACAAA TCCGCCGCTC ATCTTTCGCA ATGAACTTAT TGAGCACTTC 
AAGGAGTTTG GCACCGAGCT GTATGCCCGC ATGCGGGCCT GGTCGGAGTA TTCTCTTGAG 
GCACAGCGCC AAAATATAAC CAAAATCAAG GACATGCCTG TGGAGTACAA GGCGTCTTGC 
GAGCTACCCG AATTCCCGCT GCTACCATGC AGTCGAGGCT TTTGCATAGC GGTCAAGCGC 
GTTTTGAGCC AACTTCAAAG CGAGCTAACT GCCTTGGGTG CCAATCTGGT TACGGATGAA 
CGTAATGGCA TTGCGACTGG AATTGGCACT GCCCCGCGCG CTGCTCCGGA GGAGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG10254 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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