pnt cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	pnt
Entrez Gene ID	42757
Full Name	pointed
Synonyms	0123/09,0608/07,0998/12,3520,CG17077,D-Ets-2,D-ets-2,DMPOINT1A,Dmel\CG17077,E(E2F)3D,EC3-2,EK3-2,ETS2,EY3-1,Ets,Ets2,Ets58AB,Ets94F,PNT-P1,PNTP1,PNTP2,POINT,Pnt,Pnt-P1,Pnt2,PntP1,PntP2,Pointed-P1,ets94F,l(3)07825,l(3)j1B7,l(3)s118306,png,pnt-P1,pnt-P2,pntP2,pntegfr,pointed-RC,ptd
General protein information	Preferred Names pointed Names CG17077 gene product from transcript CG17077-RB CG17077-PB CG17077-PC CG17077-PD CG17077-PE Ets at 58AB Ets protein pointed P2 Point2 Pointed2 no terminal cell clones-R pnt-PB pnt-PC pnt-PD pnt-PE pointed P2 pointed-P1 pointed-P2 pointedP2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 94E10-94E13\|3-79.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275943.1	NP_001262872.1	pointed, isoform E
NM_079737.3	NP_524461.2	pointed, isoform B
NM_170070.2	NP_732858.1	pointed, isoform C
NM_170651.4	NP_732857.1	pointed, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Both PntP1 and PntP2 are required for oenocyte specification. Since PntP1 is a downstream effector of EGF signaling, these findings provide insight into how a Hox factor can both trigger and potentiate the EGF signal to promote an essential cell fate along the body plan.
Title: A Hox complex activates and potentiates the Epidermal Growth Factor signaling pathway to specify Drosophila oenocytes.

results demonstrate that PntP1 prevents both the premature differentiation and the dedifferentiation of INPs by regulating the expression of distinct target genes at different stages of imINP development.
Title: The Ets protein Pointed prevents both premature differentiation and dedifferentiation of Drosophila intermediate neural progenitors.

These studies identify Cic, Pnt, and Ets21C as critical downstream effectors of EGFR signaling in Drosophila Intestinal Stem Cells
Title: EGFR/Ras Signaling Controls Drosophila Intestinal Stem Cell Proliferation via Capicua-Regulated Genes.

PntP2 transcription factor plays a role in air sac development by regulation of the EGF ligand Vein protein.
Title: FGF coordinates air sac development by activation of the EGF ligand Vein through the transcription factor PntP2.

Btd prevents the premature differentiation by suppressing the expression of Prospero in immature intermediate neural progenitor cells. Btd functions cooperatively with Pointed P1 to promote the generation of the above cells.
Title: The Drosophila Sp8 transcription factor Buttonhead prevents premature differentiation of intermediate neural progenitors.

The following pnt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pnt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05779	NM_079737.3 Latest version!	Drosophila melanogaster pointed (pnt), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa05780	NM_170651.4 Latest version!	Drosophila melanogaster pointed (pnt), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa05781	NM_170070.2 Latest version!	Drosophila melanogaster pointed (pnt), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa07604	NM_001275943.1 Latest version!	Drosophila melanogaster pointed (pnt), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pnt ( NM_079737.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524461.2 Drosophila melanogaster pointed (pnt), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa05779
Clone ID Related Accession (Same CDS sequence)	NM_079737.3
Accession Version	NM_079737.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pointed, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079737.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAATTGG CGATTTGTAA AACAGATCTG TCTGCCACGA AATTTATGCT GCCGCCCGCT 
TTGCCCTCCT CAGCGGCCAT TGGTTCCTCG TCGGCGGTCG CCTCCACGGC CTCGCATTTC 
CTGGATAAGG CGGCCCACGA GCTCTTTCAA CTGAACGCCA TCAACGGACA CCTGTTCAAG 
TCGCCGGCCA GCAGCCACCT CAATTCGGTC GGCAGTCCGT CGATACTTAG CCAATTGAAC 
GGCATCGGCA ACAGCGGCAA CCATTCGGGC CAAGTTAGCA CCATGAGGCT CAAGAAGAAC 
CGCAAAGTCA CCTTTCTGTC CAGCCTAGTT GAGTCGAAAA ATATTTTCAT CAAGGAGGAG 
CCGATCCATG GATGCAAGGA TCTGTGCAGT TTGTCGGATA TTTCAGATCA TGAGGCATCA 
TTAGAAGTTC CCACCGCCTT GCCACCCCTC ACGCCGGGCA CCAATCGCAA GGTCAACGAG 
GTACTGAAGG CATCCTTTGC CTCCTGGGAA AAGGAGGTCC AAAAATGCAA CATAACCAAA 
GATCCGCGTG AATGGACGGA GGAGCATGTC ATCTACTGGC TCAATTGGGC TAAAAATGAG 
TTCTCATTGG TCTCCATGAA CCTGGACCCC TTCTACAAGA TGAAGGGACG CGCCATGGTG 
GATCTGGGCA AGGAGAAGTT CCTGGCCATC ACGCCGCCCT TCACCGGCGA CATACTGTGG 
GAGCACTTGG ACATCCTGCA AAAAGATTGC GAGAAACCAA ATGAGGATAT TGTGCACGGC 
AATTCCTTTG AGTCGGCCAC CACGGCCTCG GTCTGTGGAT CGGACCACCA GGTGGCCAAT 
TACCCCAACG AGACGCACGC CAACAGCAAC AACAGCAGCA TAAACAGCCG CCTGTCCATG 
GATTATGTGA CGTCGGCGGG GAATAGTGAT AATAAAAACT TCCACCGCGC CACGCCCCAC 
TCCCACAATG GCTACAACAC CAGCAACACA CACGACCGCA TCAACAACAG CACGCCCCCG 
CAGCAGCAGC AATCGCAGCA GCCGACTGTC AATGGCAGCG GTAGCGCCAG CAGCAACAAC 
AATAACTCCA TGCTACCGCC CGCTGTACAG CAAAGCAACA ACGAAAATAA CAACACCAGC 
AGCAGCAACA CAAACAACAG CAGCAACAAC AACAACAACA GCGGTGGCAG CAACAATTCG 
AATGCGGGCA GCAATAACAA CAATAATAAC AACAATAATA TTAACTTTAT GGCAGCGGCG 
GCGATCTTCC AACATCACCT CAAAGAGGAG CCGGGCACCC AGAATGGTAA TATCGGTGGC 
TATGGTGGTG GCAGTAATAG CCAGAACGAT CCCACGGATC TCAGTAGTTA TGGATTGCCC 
GCCCACCTGG CCGCTTACGG TGGTGGGAGT GGCAGTGGGC CAACCGGCGG CAGGAGCTCC 
GGTGGCGGTG GCGATGAAAG CGATTATCAC AGCACGATCT CGGCGCAGGA TCACCAGAGT 
CAGCAATCGA GTGGAGGAAA TGGCAGTGGA GGAGCCAGCG GTGGAAGCAC CGGAAACAGC 
AACGGTTACT TGGACAGCAG CTCGGAGTTC TATGGCAGTT ACGCGGGCAG GAATCGATTC 
CACGATGGTT ATCCACCGGA ATTTACGCCC TATGATGCTC AATCCTTCCA GTCGATGGGC 
CCCCAGCCCA CGGCCATGGA TCAGTGGGGA GCAGCCCATG CCCACCAGCA TCCGGCAGCG 
TATATGAGCA CCTTGGGTCT GGATAAGGGC CTGCTGGGTG GCTATACGAC GCAGGGCGGA 
GTGCCGTGCT TCACGGGATC GGGTCCCATT CAACTGTGGC AATTCCTGCT CGAATTGCTG 
CTGGACAAGA CGTGTCAGAG TTTCATTTCG TGGACCGGCG ATGGCTGGGA GTTCAAGCTA 
ACAGATCCTG ATGAGGTGGC TCGTCGTTGG GGAATTCGCA AAAACAAACC GAAGATGAAC 
TACGAGAAGC TGAGTCGCGG TCTGCGCTAC TATTATGACA AAAACATCAT CCACAAGACG 
GCAGGCAAAC GATATGTCTA CCGCTTTGTC TGTGATCTCC AGAATCTAGT TGGGCACACA 
CCCGAGGAGC TGGTGGCCAA GTATGATCTT AAGATTGAGA AAAAAGATGT GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524461.2
CDS	784..2940
Translation	MELAICKTDLSATKFMLPPALPSSAAIGSSSAVASTASHFLDKAAHELFQLNAINGHLFKSPASSHLNSVGSPSILSQLNGIGNSGNHSGQVSTMRLKKNRKVTFLSSLVESKNIFIKEEPIHGCKDLCSLSDISDHEASLEVPTALPPLTPGTNRKVNEVLKASFASWEKEVQKCNITKDPREWTEEHVIYWLNWAKNEFSLVSMNLDPFYKMKGRAMVDLGKEKFLAITPPFTGDILWEHLDILQKDCEKPNEDIVHGNSFESATTASVCGSDHQVANYPNETHANSNNSSINSRLSMDYVTSAGNSDNKNFHRATPHSHNGYNTSNTHDRINNSTPPQQQQSQQPTVNGSGSASSNNNNSMLPPAVQQSNNENNNTSSSNTNNSSNNNNNSGGSNNSNAGSNNNNNNNNNINFMAAAAIFQHHLKEEPGTQNGNIGGYGGGSNSQNDPTDLSSYGLPAHLAAYGGGSGSGPTGGRSSGGGGDESDYHSTISAQDHQSQQSSGGNGSGGASGGSTGNSNGYLDSSSEFYGSYAGRNRFHDGYPPEFTPYDAQSFQSMGPQPTAMDQWGAAHAHQHPAAYMSTLGLDKGLLGGYTTQGGVPCFTGSGPIQLWQFLLELLLDKTCQSFISWTGDGWEFKLTDPDEVARRWGIRKNKPKMNYEKLSRGLRYYYDKNIIHKTAGKRYVYRFVCDLQNLVGHTPEELVAKYDLKIEKKDVD

Target ORF information:

RefSeq Version	NM_079737.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pointed (pnt), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079737.3

ORF Insert Sequence:

ATGGAATTGG CGATTTGTAA AACAGATCTG TCTGCCACGA AATTTATGCT GCCGCCCGCT 
TTGCCCTCCT CAGCGGCCAT TGGTTCCTCG TCGGCGGTCG CCTCCACGGC CTCGCATTTC 
CTGGATAAGG CGGCCCACGA GCTCTTTCAA CTGAACGCCA TCAACGGACA CCTGTTCAAG 
TCGCCGGCCA GCAGCCACCT CAATTCGGTC GGCAGTCCGT CGATACTTAG CCAATTGAAC 
GGCATCGGCA ACAGCGGCAA CCATTCGGGC CAAGTTAGCA CCATGAGGCT CAAGAAGAAC 
CGCAAAGTCA CCTTTCTGTC CAGCCTAGTT GAGTCGAAAA ATATTTTCAT CAAGGAGGAG 
CCGATCCATG GATGCAAGGA TCTGTGCAGT TTGTCGGATA TTTCAGATCA TGAGGCATCA 
TTAGAAGTTC CCACCGCCTT GCCACCCCTC ACGCCGGGCA CCAATCGCAA GGTCAACGAG 
GTACTGAAGG CATCCTTTGC CTCCTGGGAA AAGGAGGTCC AAAAATGCAA CATAACCAAA 
GATCCGCGTG AATGGACGGA GGAGCATGTC ATCTACTGGC TCAATTGGGC TAAAAATGAG 
TTCTCATTGG TCTCCATGAA CCTGGACCCC TTCTACAAGA TGAAGGGACG CGCCATGGTG 
GATCTGGGCA AGGAGAAGTT CCTGGCCATC ACGCCGCCCT TCACCGGCGA CATACTGTGG 
GAGCACTTGG ACATCCTGCA AAAAGATTGC GAGAAACCAA ATGAGGATAT TGTGCACGGC 
AATTCCTTTG AGTCGGCCAC CACGGCCTCG GTCTGTGGAT CGGACCACCA GGTGGCCAAT 
TACCCCAACG AGACGCACGC CAACAGCAAC AACAGCAGCA TAAACAGCCG CCTGTCCATG 
GATTATGTGA CGTCGGCGGG GAATAGTGAT AATAAAAACT TCCACCGCGC CACGCCCCAC 
TCCCACAATG GCTACAACAC CAGCAACACA CACGACCGCA TCAACAACAG CACGCCCCCG 
CAGCAGCAGC AATCGCAGCA GCCGACTGTC AATGGCAGCG GTAGCGCCAG CAGCAACAAC 
AATAACTCCA TGCTACCGCC CGCTGTACAG CAAAGCAACA ACGAAAATAA CAACACCAGC 
AGCAGCAACA CAAACAACAG CAGCAACAAC AACAACAACA GCGGTGGCAG CAACAATTCG 
AATGCGGGCA GCAATAACAA CAATAATAAC AACAATAATA TTAACTTTAT GGCAGCGGCG 
GCGATCTTCC AACATCACCT CAAAGAGGAG CCGGGCACCC AGAATGGTAA TATCGGTGGC 
TATGGTGGTG GCAGTAATAG CCAGAACGAT CCCACGGATC TCAGTAGTTA TGGATTGCCC 
GCCCACCTGG CCGCTTACGG TGGTGGGAGT GGCAGTGGGC CAACCGGCGG CAGGAGCTCC 
GGTGGCGGTG GCGATGAAAG CGATTATCAC AGCACGATCT CGGCGCAGGA TCACCAGAGT 
CAGCAATCGA GTGGAGGAAA TGGCAGTGGA GGAGCCAGCG GTGGAAGCAC CGGAAACAGC 
AACGGTTACT TGGACAGCAG CTCGGAGTTC TATGGCAGTT ACGCGGGCAG GAATCGATTC 
CACGATGGTT ATCCACCGGA ATTTACGCCC TATGATGCTC AATCCTTCCA GTCGATGGGC 
CCCCAGCCCA CGGCCATGGA TCAGTGGGGA GCAGCCCATG CCCACCAGCA TCCGGCAGCG 
TATATGAGCA CCTTGGGTCT GGATAAGGGC CTGCTGGGTG GCTATACGAC GCAGGGCGGA 
GTGCCGTGCT TCACGGGATC GGGTCCCATT CAACTGTGGC AATTCCTGCT CGAATTGCTG 
CTGGACAAGA CGTGTCAGAG TTTCATTTCG TGGACCGGCG ATGGCTGGGA GTTCAAGCTA 
ACAGATCCTG ATGAGGTGGC TCGTCGTTGG GGAATTCGCA AAAACAAACC GAAGATGAAC 
TACGAGAAGC TGAGTCGCGG TCTGCGCTAC TATTATGACA AAAACATCAT CCACAAGACG 
GCAGGCAAAC GATATGTCTA CCGCTTTGTC TGTGATCTCC AGAATCTAGT TGGGCACACA 
CCCGAGGAGC TGGTGGCCAA GTATGATCTT AAGATTGAGA AAAAAGATGT GGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pnt ( NM_170651.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732857.1 Drosophila melanogaster pointed (pnt), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa05780
Clone ID Related Accession (Same CDS sequence)	NM_170651.4
Accession Version	NM_170651.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pointed, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170651.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCAATG AGTGGATCGA TTGGAATGAC AGTCGGATGC TACCGCCGCT GCGGTCGGCA 
AACTATAACT ACCATCCTCA GACCTTCTTG CCCAATAATT ATCAGTGCTT TACTGGAAAA 
TTCCACCTGA AGGGACAAAA GTTGCAGCAG CTAACCACCA ATCATAGCAA GCTCAAAGAA 
GTTCCCACCG CCTTGCCACC CCTCACGCCG GGCACCAATC GCAAGGTCAA CGAGGTACTG 
AAGGCATCCT TTGCCTCCTG GGAAAAGGAG GTCCAAAAAT GCAACATAAC CAAAGATCCG 
CGTGAATGGA CGGAGGAGCA TGTCATCTAC TGGCTCAATT GGGCTAAAAA TGAGTTCTCA 
TTGGTCTCCA TGAACCTGGA CCCCTTCTAC AAGATGAAGG GACGCGCCAT GGTGGATCTG 
GGCAAGGAGA AGTTCCTGGC CATCACGCCG CCCTTCACCG GCGACATACT GTGGGAGCAC 
TTGGACATCC TGCAAAAAGA TTGCGAGAAA CCAAATGAGG ATATTGTGCA CGGCAATTCC 
TTTGAGTCGG CCACCACGGC CTCGGTCTGT GGATCGGACC ACCAGGTGGC CAATTACCCC 
AACGAGACGC ACGCCAACAG CAACAACAGC AGCATAAACA GCCGCCTGTC CATGGATTAT 
GTGACGTCGG CGGGGAATAG TGATAATAAA AACTTCCACC GCGCCACGCC CCACTCCCAC 
AATGGCTACA ACACCAGCAA CACACACGAC CGCATCAACA ACAGCACGCC CCCGCAGCAG 
CAGCAATCGC AGCAGCCGAC TGTCAATGGC AGCGGTAGCG CCAGCAGCAA CAACAATAAC 
TCCATGCTAC CGCCCGCTGT ACAGCAAAGC AACAACGAAA ATAACAACAC CAGCAGCAGC 
AACACAAACA ACAGCAGCAA CAACAACAAC AACAGCGGTG GCAGCAACAA TTCGAATGCG 
GGCAGCAATA ACAACAATAA TAACAACAAT AATATTAACT TTATGGCAGC GGCGGCGATC 
TTCCAACATC ACCTCAAAGA GGAGCCGGGC ACCCAGAATG GTAATATCGG TGGCTATGGT 
GGTGGCAGTA ATAGCCAGAA CGATCCCACG GATCTCAGTA GTTATGGATT GCCCGCCCAC 
CTGGCCGCTT ACGGTGGTGG GAGTGGCAGT GGGCCAACCG GCGGCAGGAG CTCCGGTGGC 
GGTGGCGATG AAAGCGATTA TCACAGCACG ATCTCGGCGC AGGATCACCA GAGTCAGCAA 
TCGAGTGGAG GAAATGGCAG TGGAGGAGCC AGCGGTGGAA GCACCGGAAA CAGCAACGGT 
TACTTGGACA GCAGCTCGGA GTTCTATGGC AGTTACGCGG GCAGGAATCG ATTCCACGAT 
GGTTATCCAC CGGAATTTAC GCCCTATGAT GCTCAATCCT TCCAGTCGAT GGGCCCCCAG 
CCCACGGCCA TGGATCAGTG GGGAGCAGCC CATGCCCACC AGCATCCGGC AGCGTATATG 
AGCACCTTGG GTCTGGATAA GGGCCTGCTG GGTGGCTATA CGACGCAGGG CGGAGTGCCG 
TGCTTCACGG GATCGGGTCC CATTCAACTG TGGCAATTCC TGCTCGAATT GCTGCTGGAC 
AAGACGTGTC AGAGTTTCAT TTCGTGGACC GGCGATGGCT GGGAGTTCAA GCTAACAGAT 
CCTGATGAGG TGGCTCGTCG TTGGGGAATT CGCAAAAACA AACCGAAGAT GAACTACGAG 
AAGCTGAGTC GCGGTCTGCG CTACTATTAT GACAAAAACA TCATCCACAA GACGGCAGGC 
AAACGATATG TCTACCGCTT TGTCTGTGAT CTCCAGAATC TAGTTGGGCA CACACCCGAG 
GAGCTGGTGG CCAAGTATGA TCTTAAGATT GAGAAAAAAG ATGTGGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732857.1
CDS	474..2384
Translation	MTNEWIDWNDSRMLPPLRSANYNYHPQTFLPNNYQCFTGKFHLKGQKLQQLTTNHSKLKEVPTALPPLTPGTNRKVNEVLKASFASWEKEVQKCNITKDPREWTEEHVIYWLNWAKNEFSLVSMNLDPFYKMKGRAMVDLGKEKFLAITPPFTGDILWEHLDILQKDCEKPNEDIVHGNSFESATTASVCGSDHQVANYPNETHANSNNSSINSRLSMDYVTSAGNSDNKNFHRATPHSHNGYNTSNTHDRINNSTPPQQQQSQQPTVNGSGSASSNNNNSMLPPAVQQSNNENNNTSSSNTNNSSNNNNNSGGSNNSNAGSNNNNNNNNNINFMAAAAIFQHHLKEEPGTQNGNIGGYGGGSNSQNDPTDLSSYGLPAHLAAYGGGSGSGPTGGRSSGGGGDESDYHSTISAQDHQSQQSSGGNGSGGASGGSTGNSNGYLDSSSEFYGSYAGRNRFHDGYPPEFTPYDAQSFQSMGPQPTAMDQWGAAHAHQHPAAYMSTLGLDKGLLGGYTTQGGVPCFTGSGPIQLWQFLLELLLDKTCQSFISWTGDGWEFKLTDPDEVARRWGIRKNKPKMNYEKLSRGLRYYYDKNIIHKTAGKRYVYRFVCDLQNLVGHTPEELVAKYDLKIEKKDVD

Target ORF information:

RefSeq Version	NM_170651.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pointed (pnt), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170651.4

ORF Insert Sequence:

ATGACCAATG AGTGGATCGA TTGGAATGAC AGTCGGATGC TACCGCCGCT GCGGTCGGCA 
AACTATAACT ACCATCCTCA GACCTTCTTG CCCAATAATT ATCAGTGCTT TACTGGAAAA 
TTCCACCTGA AGGGACAAAA GTTGCAGCAG CTAACCACCA ATCATAGCAA GCTCAAAGAA 
GTTCCCACCG CCTTGCCACC CCTCACGCCG GGCACCAATC GCAAGGTCAA CGAGGTACTG 
AAGGCATCCT TTGCCTCCTG GGAAAAGGAG GTCCAAAAAT GCAACATAAC CAAAGATCCG 
CGTGAATGGA CGGAGGAGCA TGTCATCTAC TGGCTCAATT GGGCTAAAAA TGAGTTCTCA 
TTGGTCTCCA TGAACCTGGA CCCCTTCTAC AAGATGAAGG GACGCGCCAT GGTGGATCTG 
GGCAAGGAGA AGTTCCTGGC CATCACGCCG CCCTTCACCG GCGACATACT GTGGGAGCAC 
TTGGACATCC TGCAAAAAGA TTGCGAGAAA CCAAATGAGG ATATTGTGCA CGGCAATTCC 
TTTGAGTCGG CCACCACGGC CTCGGTCTGT GGATCGGACC ACCAGGTGGC CAATTACCCC 
AACGAGACGC ACGCCAACAG CAACAACAGC AGCATAAACA GCCGCCTGTC CATGGATTAT 
GTGACGTCGG CGGGGAATAG TGATAATAAA AACTTCCACC GCGCCACGCC CCACTCCCAC 
AATGGCTACA ACACCAGCAA CACACACGAC CGCATCAACA ACAGCACGCC CCCGCAGCAG 
CAGCAATCGC AGCAGCCGAC TGTCAATGGC AGCGGTAGCG CCAGCAGCAA CAACAATAAC 
TCCATGCTAC CGCCCGCTGT ACAGCAAAGC AACAACGAAA ATAACAACAC CAGCAGCAGC 
AACACAAACA ACAGCAGCAA CAACAACAAC AACAGCGGTG GCAGCAACAA TTCGAATGCG 
GGCAGCAATA ACAACAATAA TAACAACAAT AATATTAACT TTATGGCAGC GGCGGCGATC 
TTCCAACATC ACCTCAAAGA GGAGCCGGGC ACCCAGAATG GTAATATCGG TGGCTATGGT 
GGTGGCAGTA ATAGCCAGAA CGATCCCACG GATCTCAGTA GTTATGGATT GCCCGCCCAC 
CTGGCCGCTT ACGGTGGTGG GAGTGGCAGT GGGCCAACCG GCGGCAGGAG CTCCGGTGGC 
GGTGGCGATG AAAGCGATTA TCACAGCACG ATCTCGGCGC AGGATCACCA GAGTCAGCAA 
TCGAGTGGAG GAAATGGCAG TGGAGGAGCC AGCGGTGGAA GCACCGGAAA CAGCAACGGT 
TACTTGGACA GCAGCTCGGA GTTCTATGGC AGTTACGCGG GCAGGAATCG ATTCCACGAT 
GGTTATCCAC CGGAATTTAC GCCCTATGAT GCTCAATCCT TCCAGTCGAT GGGCCCCCAG 
CCCACGGCCA TGGATCAGTG GGGAGCAGCC CATGCCCACC AGCATCCGGC AGCGTATATG 
AGCACCTTGG GTCTGGATAA GGGCCTGCTG GGTGGCTATA CGACGCAGGG CGGAGTGCCG 
TGCTTCACGG GATCGGGTCC CATTCAACTG TGGCAATTCC TGCTCGAATT GCTGCTGGAC 
AAGACGTGTC AGAGTTTCAT TTCGTGGACC GGCGATGGCT GGGAGTTCAA GCTAACAGAT 
CCTGATGAGG TGGCTCGTCG TTGGGGAATT CGCAAAAACA AACCGAAGAT GAACTACGAG 
AAGCTGAGTC GCGGTCTGCG CTACTATTAT GACAAAAACA TCATCCACAA GACGGCAGGC 
AAACGATATG TCTACCGCTT TGTCTGTGAT CTCCAGAATC TAGTTGGGCA CACACCCGAG 
GAGCTGGTGG CCAAGTATGA TCTTAAGATT GAGAAAAAAG ATGTGGATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pnt ( NM_170070.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732858.1 Drosophila melanogaster pointed (pnt), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa05781
Clone ID Related Accession (Same CDS sequence)	NM_170070.2
Accession Version	NM_170070.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pointed, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170070.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGCCCT CTGCGTTTTT AGTGAATGCC AGCATAATCA GTGCCGCCAA CGCCTTGGAC 
AGCGATAAGG AGCAGCAGCT TCAGGAGCAT CTCAATCAGA GTCACTTCAC CAGCAGCAGC 
AGCAGCAGCA ACAGCAGCAA CAATAACAAT TGCCACACGG CGCCCAACTA CAACATGGTC 
CTGCAAAGCT ATGAGAACTA TCCCAGCTAC CACCTGGCTC AGCATCATCA TCATCATCAT 
CATCACCACA GCCATCCGCA TCACCAATTG GCGCAGCAGA CAGGGCTGCA CAGCCATCAT 
ACGATGCAAC AGCAGCTGCA GCAGCAGCAG CAATCCCTAC TCCTGCAACA CCCGCAACAA 
CAGCAGCAGC AGCACAGCCA CCAGTCGCAA CAGCAGCAGC AACACGGATA CGGATCATCA 
GCCCAGCTGC CCCATCATCG TTTATCCGGC GGCAGTACGG GCAGCACCAC CACCACCTCC 
TCCGGCTCCA GTTCGAGTGG CTCCTCGTCG AGTGCCTCAT CGCAGGGATT CGCCAACGGG 
AGCGCGACTG CGAACAATCT GGTGGGGGCG TTGAGCAGCT CCACGGCTGC TTCGCTGGGA 
CTGGGCTACT TCAATGATAT GGCGCCCTTT GTGGGCGATG CGAATGCCTA CTACACGGAC 
TCGGATGTCA ACTTCTTTAG TTCAGGCTAC AACACCAGCA ACACACACGA CCGCATCAAC 
AACAGCACGC CCCCGCAGCA GCAGCAATCG CAGCAGCCGA CTGTCAATGG CAGCGGTAGC 
GCCAGCAGCA ACAACAATAA CTCCATGCTA CCGCCCGCTG TACAGCAAAG CAACAACGAA 
AATAACAACA CCAGCAGCAG CAACACAAAC AACAGCAGCA ACAACAACAA CAACAGCGGT 
GGCAGCAACA ATTCGAATGC GGGCAGCAAT AACAACAATA ATAACAACAA TAATATTAAC 
TTTATGGCAG CGGCGGCGAT CTTCCAACAT CACCTCAAAG AGGAGCCGGG CACCCAGAAT 
GGTAATATCG GTGGCTATGG TGGTGGCAGT AATAGCCAGA ACGATCCCAC GGATCTCAGT 
AGTTATGGAT TGCCCGCCCA CCTGGCCGCT TACGGTGGTG GGAGTGGCAG TGGGCCAACC 
GGCGGCAGGA GCTCCGGTGG CGGTGGCGAT GAAAGCGATT ATCACAGCAC GATCTCGGCG 
CAGGATCACC AGAGTCAGCA ATCGAGTGGA GGAAATGGCA GTGGAGGAGC CAGCGGTGGA 
AGCACCGGAA ACAGCAACGG TTACTTGGAC AGCAGCTCGG AGTTCTATGG CAGTTACGCG 
GGCAGGAATC GATTCCACGA TGGTTATCCA CCGGAATTTA CGCCCTATGA TGCTCAATCC 
TTCCAGTCGA TGGGCCCCCA GCCCACGGCC ATGGATCAGT GGGGAGCAGC CCATGCCCAC 
CAGCATCCGG CAGCGTATAT GAGCACCTTG GGTCTGGATA AGGGCCTGCT GGGTGGCTAT 
ACGACGCAGG GCGGAGTGCC GTGCTTCACG GGATCGGGTC CCATTCAACT GTGGCAATTC 
CTGCTCGAAT TGCTGCTGGA CAAGACGTGT CAGAGTTTCA TTTCGTGGAC CGGCGATGGC 
TGGGAGTTCA AGCTAACAGA TCCTGATGAG GTGGCTCGTC GTTGGGGAAT TCGCAAAAAC 
AAACCGAAGA TGAACTACGA GAAGCTGAGT CGCGGTCTGC GCTACTATTA TGACAAAAAC 
ATCATCCACA AGACGGCAGG CAAACGATAT GTCTACCGCT TTGTCTGTGA TCTCCAGAAT 
CTAGTTGGGC ACACACCCGA GGAGCTGGTG GCCAAGTATG ATCTTAAGAT TGAGAAAAAA 
GATGTGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732858.1
CDS	704..2575
Translation	MPPSAFLVNASIISAANALDSDKEQQLQEHLNQSHFTSSSSSSNSSNNNNCHTAPNYNMVLQSYENYPSYHLAQHHHHHHHHHSHPHHQLAQQTGLHSHHTMQQQLQQQQQSLLLQHPQQQQQQHSHQSQQQQQHGYGSSAQLPHHRLSGGSTGSTTTTSSGSSSSGSSSSASSQGFANGSATANNLVGALSSSTAASLGLGYFNDMAPFVGDANAYYTDSDVNFFSSGYNTSNTHDRINNSTPPQQQQSQQPTVNGSGSASSNNNNSMLPPAVQQSNNENNNTSSSNTNNSSNNNNNSGGSNNSNAGSNNNNNNNNNINFMAAAAIFQHHLKEEPGTQNGNIGGYGGGSNSQNDPTDLSSYGLPAHLAAYGGGSGSGPTGGRSSGGGGDESDYHSTISAQDHQSQQSSGGNGSGGASGGSTGNSNGYLDSSSEFYGSYAGRNRFHDGYPPEFTPYDAQSFQSMGPQPTAMDQWGAAHAHQHPAAYMSTLGLDKGLLGGYTTQGGVPCFTGSGPIQLWQFLLELLLDKTCQSFISWTGDGWEFKLTDPDEVARRWGIRKNKPKMNYEKLSRGLRYYYDKNIIHKTAGKRYVYRFVCDLQNLVGHTPEELVAKYDLKIEKKDVD

Target ORF information:

RefSeq Version	NM_170070.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pointed (pnt), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170070.2

ORF Insert Sequence:

ATGCCGCCCT CTGCGTTTTT AGTGAATGCC AGCATAATCA GTGCCGCCAA CGCCTTGGAC 
AGCGATAAGG AGCAGCAGCT TCAGGAGCAT CTCAATCAGA GTCACTTCAC CAGCAGCAGC 
AGCAGCAGCA ACAGCAGCAA CAATAACAAT TGCCACACGG CGCCCAACTA CAACATGGTC 
CTGCAAAGCT ATGAGAACTA TCCCAGCTAC CACCTGGCTC AGCATCATCA TCATCATCAT 
CATCACCACA GCCATCCGCA TCACCAATTG GCGCAGCAGA CAGGGCTGCA CAGCCATCAT 
ACGATGCAAC AGCAGCTGCA GCAGCAGCAG CAATCCCTAC TCCTGCAACA CCCGCAACAA 
CAGCAGCAGC AGCACAGCCA CCAGTCGCAA CAGCAGCAGC AACACGGATA CGGATCATCA 
GCCCAGCTGC CCCATCATCG TTTATCCGGC GGCAGTACGG GCAGCACCAC CACCACCTCC 
TCCGGCTCCA GTTCGAGTGG CTCCTCGTCG AGTGCCTCAT CGCAGGGATT CGCCAACGGG 
AGCGCGACTG CGAACAATCT GGTGGGGGCG TTGAGCAGCT CCACGGCTGC TTCGCTGGGA 
CTGGGCTACT TCAATGATAT GGCGCCCTTT GTGGGCGATG CGAATGCCTA CTACACGGAC 
TCGGATGTCA ACTTCTTTAG TTCAGGCTAC AACACCAGCA ACACACACGA CCGCATCAAC 
AACAGCACGC CCCCGCAGCA GCAGCAATCG CAGCAGCCGA CTGTCAATGG CAGCGGTAGC 
GCCAGCAGCA ACAACAATAA CTCCATGCTA CCGCCCGCTG TACAGCAAAG CAACAACGAA 
AATAACAACA CCAGCAGCAG CAACACAAAC AACAGCAGCA ACAACAACAA CAACAGCGGT 
GGCAGCAACA ATTCGAATGC GGGCAGCAAT AACAACAATA ATAACAACAA TAATATTAAC 
TTTATGGCAG CGGCGGCGAT CTTCCAACAT CACCTCAAAG AGGAGCCGGG CACCCAGAAT 
GGTAATATCG GTGGCTATGG TGGTGGCAGT AATAGCCAGA ACGATCCCAC GGATCTCAGT 
AGTTATGGAT TGCCCGCCCA CCTGGCCGCT TACGGTGGTG GGAGTGGCAG TGGGCCAACC 
GGCGGCAGGA GCTCCGGTGG CGGTGGCGAT GAAAGCGATT ATCACAGCAC GATCTCGGCG 
CAGGATCACC AGAGTCAGCA ATCGAGTGGA GGAAATGGCA GTGGAGGAGC CAGCGGTGGA 
AGCACCGGAA ACAGCAACGG TTACTTGGAC AGCAGCTCGG AGTTCTATGG CAGTTACGCG 
GGCAGGAATC GATTCCACGA TGGTTATCCA CCGGAATTTA CGCCCTATGA TGCTCAATCC 
TTCCAGTCGA TGGGCCCCCA GCCCACGGCC ATGGATCAGT GGGGAGCAGC CCATGCCCAC 
CAGCATCCGG CAGCGTATAT GAGCACCTTG GGTCTGGATA AGGGCCTGCT GGGTGGCTAT 
ACGACGCAGG GCGGAGTGCC GTGCTTCACG GGATCGGGTC CCATTCAACT GTGGCAATTC 
CTGCTCGAAT TGCTGCTGGA CAAGACGTGT CAGAGTTTCA TTTCGTGGAC CGGCGATGGC 
TGGGAGTTCA AGCTAACAGA TCCTGATGAG GTGGCTCGTC GTTGGGGAAT TCGCAAAAAC 
AAACCGAAGA TGAACTACGA GAAGCTGAGT CGCGGTCTGC GCTACTATTA TGACAAAAAC 
ATCATCCACA AGACGGCAGG CAAACGATAT GTCTACCGCT TTGTCTGTGA TCTCCAGAAT 
CTAGTTGGGC ACACACCCGA GGAGCTGGTG GCCAAGTATG ATCTTAAGAT TGAGAAAAAA 
GATGTGGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pnt ( NM_001275943.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262872.1 Drosophila melanogaster pointed (pnt), transcript variant E, mRNA.

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CloneID	OFa07604
Clone ID Related Accession (Same CDS sequence)	NM_001275943.1
Accession Version	NM_001275943.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	pointed, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGCCCT CTGCGTTTTT AGTGAATGCC AGCATAATCA GTGCCGCCAA CGCCTTGGAC 
AGCGATAAGG AGCAGCAGCT TCAGGAGCAT CTCAATCAGA GTCACTTCAC CAGCAGCAGC 
AGCAGCAGCA ACAGCAGCAA CAATAACAAT TGCCACACGG CGCCCAACTA CAACATGGTC 
CTGCAAAGCT ATGAGAACTA TCCCAGCTAC CACCTGGCTC AGCATCATCA TCATCATCAT 
CATCACCACA GCCATCCGCA TCACCAATTG GCGCAGCAGA CAGGGCTGCA CAGCCATCAT 
ACGATGCAAC AGCAGCTGCA GCAGCAGCAG CAATCCCTAC TCCTGCAACA CCCGCAACAA 
CAGCAGCAGC AGCACAGCCA CCAGTCGCAA CAGCAGCAGC AACACGGATA CGGATCATCA 
GCCCAGCTGC CCCATCATCG TTTATCCGGC GGCAGTACGG GCAGCACCAC CACCACCTCC 
TCCGGCTCCA GTTCGAGTGG CTCCTCGTCG AGTGCCTCAT CGCAGGGATT CGCCAACGGG 
AGCGCGACTG CGAACAATCT GGTGGGGGCG TTGAGCAGCT CCACGGCTGC TTCGCTGGGA 
CTGGGCTACT TCAATGATAT GGCGCCCTTT GTGGGCGATG CGAATGCCTA CTACACGGAC 
TCGGATGTCA ACTTCTTTAG TTCAGGTACA GGCTACAACA CCAGCAACAC ACACGACCGC 
ATCAACAACA GCACGCCCCC GCAGCAGCAG CAATCGCAGC AGCCGACTGT CAATGGCAGC 
GGTAGCGCCA GCAGCAACAA CAATAACTCC ATGCTACCGC CCGCTGTACA GCAAAGCAAC 
AACGAAAATA ACAACACCAG CAGCAGCAAC ACAAACAACA GCAGCAACAA CAACAACAAC 
AGCGGTGGCA GCAACAATTC GAATGCGGGC AGCAATAACA ACAATAATAA CAACAATAAT 
ATTAACTTTA TGGCAGCGGC GGCGATCTTC CAACATCACC TCAAAGAGGA GCCGGGCACC 
CAGAATGGTA ATATCGGTGG CTATGGTGGT GGCAGTAATA GCCAGAACGA TCCCACGGAT 
CTCAGTAGTT ATGGATTGCC CGCCCACCTG GCCGCTTACG GTGGTGGGAG TGGCAGTGGG 
CCAACCGGCG GCAGGAGCTC CGGTGGCGGT GGCGATGAAA GCGATTATCA CAGCACGATC 
TCGGCGCAGG ATCACCAGAG TCAGCAATCG AGTGGAGGAA ATGGCAGTGG AGGAGCCAGC 
GGTGGAAGCA CCGGAAACAG CAACGGTTAC TTGGACAGCA GCTCGGAGTT CTATGGCAGT 
TACGCGGGCA GGAATCGATT CCACGATGGT TATCCACCGG AATTTACGCC CTATGATGCT 
CAATCCTTCC AGTCGATGGG CCCCCAGCCC ACGGCCATGG ATCAGTGGGG AGCAGCCCAT 
GCCCACCAGC ATCCGGCAGC GTATATGAGC ACCTTGGGTC TGGATAAGGG CCTGCTGGGT 
GGCTATACGA CGCAGGGCGG AGTGCCGTGC TTCACGGGAT CGGGTCCCAT TCAACTGTGG 
CAATTCCTGC TCGAATTGCT GCTGGACAAG ACGTGTCAGA GTTTCATTTC GTGGACCGGC 
GATGGCTGGG AGTTCAAGCT AACAGATCCT GATGAGGTGG CTCGTCGTTG GGGAATTCGC 
AAAAACAAAC CGAAGATGAA CTACGAGAAG CTGAGTCGCG GTCTGCGCTA CTATTATGAC 
AAAAACATCA TCCACAAGAC GGCAGGCAAA CGATATGTCT ACCGCTTTGT CTGTGATCTC 
CAGAATCTAG TTGGGCACAC ACCCGAGGAG CTGGTGGCCA AGTATGATCT TAAGATTGAG 
AAAAAAGATG TGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262872.1
CDS	704..2581
Translation	MPPSAFLVNASIISAANALDSDKEQQLQEHLNQSHFTSSSSSSNSSNNNNCHTAPNYNMVLQSYENYPSYHLAQHHHHHHHHHSHPHHQLAQQTGLHSHHTMQQQLQQQQQSLLLQHPQQQQQQHSHQSQQQQQHGYGSSAQLPHHRLSGGSTGSTTTTSSGSSSSGSSSSASSQGFANGSATANNLVGALSSSTAASLGLGYFNDMAPFVGDANAYYTDSDVNFFSSGTGYNTSNTHDRINNSTPPQQQQSQQPTVNGSGSASSNNNNSMLPPAVQQSNNENNNTSSSNTNNSSNNNNNSGGSNNSNAGSNNNNNNNNNINFMAAAAIFQHHLKEEPGTQNGNIGGYGGGSNSQNDPTDLSSYGLPAHLAAYGGGSGSGPTGGRSSGGGGDESDYHSTISAQDHQSQQSSGGNGSGGASGGSTGNSNGYLDSSSEFYGSYAGRNRFHDGYPPEFTPYDAQSFQSMGPQPTAMDQWGAAHAHQHPAAYMSTLGLDKGLLGGYTTQGGVPCFTGSGPIQLWQFLLELLLDKTCQSFISWTGDGWEFKLTDPDEVARRWGIRKNKPKMNYEKLSRGLRYYYDKNIIHKTAGKRYVYRFVCDLQNLVGHTPEELVAKYDLKIEKKDVD

Target ORF information:

RefSeq Version	NM_001275943.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster pointed (pnt), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275943.1

ORF Insert Sequence:

ATGCCGCCCT CTGCGTTTTT AGTGAATGCC AGCATAATCA GTGCCGCCAA CGCCTTGGAC 
AGCGATAAGG AGCAGCAGCT TCAGGAGCAT CTCAATCAGA GTCACTTCAC CAGCAGCAGC 
AGCAGCAGCA ACAGCAGCAA CAATAACAAT TGCCACACGG CGCCCAACTA CAACATGGTC 
CTGCAAAGCT ATGAGAACTA TCCCAGCTAC CACCTGGCTC AGCATCATCA TCATCATCAT 
CATCACCACA GCCATCCGCA TCACCAATTG GCGCAGCAGA CAGGGCTGCA CAGCCATCAT 
ACGATGCAAC AGCAGCTGCA GCAGCAGCAG CAATCCCTAC TCCTGCAACA CCCGCAACAA 
CAGCAGCAGC AGCACAGCCA CCAGTCGCAA CAGCAGCAGC AACACGGATA CGGATCATCA 
GCCCAGCTGC CCCATCATCG TTTATCCGGC GGCAGTACGG GCAGCACCAC CACCACCTCC 
TCCGGCTCCA GTTCGAGTGG CTCCTCGTCG AGTGCCTCAT CGCAGGGATT CGCCAACGGG 
AGCGCGACTG CGAACAATCT GGTGGGGGCG TTGAGCAGCT CCACGGCTGC TTCGCTGGGA 
CTGGGCTACT TCAATGATAT GGCGCCCTTT GTGGGCGATG CGAATGCCTA CTACACGGAC 
TCGGATGTCA ACTTCTTTAG TTCAGGTACA GGCTACAACA CCAGCAACAC ACACGACCGC 
ATCAACAACA GCACGCCCCC GCAGCAGCAG CAATCGCAGC AGCCGACTGT CAATGGCAGC 
GGTAGCGCCA GCAGCAACAA CAATAACTCC ATGCTACCGC CCGCTGTACA GCAAAGCAAC 
AACGAAAATA ACAACACCAG CAGCAGCAAC ACAAACAACA GCAGCAACAA CAACAACAAC 
AGCGGTGGCA GCAACAATTC GAATGCGGGC AGCAATAACA ACAATAATAA CAACAATAAT 
ATTAACTTTA TGGCAGCGGC GGCGATCTTC CAACATCACC TCAAAGAGGA GCCGGGCACC 
CAGAATGGTA ATATCGGTGG CTATGGTGGT GGCAGTAATA GCCAGAACGA TCCCACGGAT 
CTCAGTAGTT ATGGATTGCC CGCCCACCTG GCCGCTTACG GTGGTGGGAG TGGCAGTGGG 
CCAACCGGCG GCAGGAGCTC CGGTGGCGGT GGCGATGAAA GCGATTATCA CAGCACGATC 
TCGGCGCAGG ATCACCAGAG TCAGCAATCG AGTGGAGGAA ATGGCAGTGG AGGAGCCAGC 
GGTGGAAGCA CCGGAAACAG CAACGGTTAC TTGGACAGCA GCTCGGAGTT CTATGGCAGT 
TACGCGGGCA GGAATCGATT CCACGATGGT TATCCACCGG AATTTACGCC CTATGATGCT 
CAATCCTTCC AGTCGATGGG CCCCCAGCCC ACGGCCATGG ATCAGTGGGG AGCAGCCCAT 
GCCCACCAGC ATCCGGCAGC GTATATGAGC ACCTTGGGTC TGGATAAGGG CCTGCTGGGT 
GGCTATACGA CGCAGGGCGG AGTGCCGTGC TTCACGGGAT CGGGTCCCAT TCAACTGTGG 
CAATTCCTGC TCGAATTGCT GCTGGACAAG ACGTGTCAGA GTTTCATTTC GTGGACCGGC 
GATGGCTGGG AGTTCAAGCT AACAGATCCT GATGAGGTGG CTCGTCGTTG GGGAATTCGC 
AAAAACAAAC CGAAGATGAA CTACGAGAAG CTGAGTCGCG GTCTGCGCTA CTATTATGAC 
AAAAACATCA TCCACAAGAC GGCAGGCAAA CGATATGTCT ACCGCTTTGT CTGTGATCTC 
CAGAATCTAG TTGGGCACAC ACCCGAGGAG CTGGTGGCCA AGTATGATCT TAAGATTGAG 
AAAAAAGATG TGGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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pnt cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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