Rad60 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rad60
Entrez Gene ID	42748
Full Name	CG4449 gene product from transcript CG4449-RB
Synonyms	CG4449,Dmel\CG4449,RENi,dRad60,drad60
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 94E8-94E9

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275939.1	NP_001262868.1	Rad60, isoform E
NM_001275940.1	NP_001262869.1	Rad60, isoform F
NM_001170227.1	NP_001163698.1	Rad60, isoform B
NM_142877.1	NP_651134.1	Rad60, isoform A
NM_001275937.1	NP_001262866.1	Rad60, isoform C
NM_001275938.1	NP_001262867.1	Rad60, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rad60 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rad60 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05767	NM_142877.1 Latest version!	Drosophila melanogaster Rad60, transcript variant A (Rad60), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa05767	NM_001275938.1 Latest version!	Drosophila melanogaster Rad60, transcript variant D (Rad60), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa07598	NM_001170227.1 Latest version!	Drosophila melanogaster Rad60, transcript variant B (Rad60), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa07598	NM_001275937.1 Latest version!	Drosophila melanogaster Rad60, transcript variant C (Rad60), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa07599	NM_001275940.1 Latest version!	Drosophila melanogaster Rad60, transcript variant F (Rad60), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa07600	NM_001275939.1 Latest version!	Drosophila melanogaster Rad60, transcript variant E (Rad60), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rad60 ( NM_142877.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651134.1 Drosophila melanogaster Rad60, transcript variant A (Rad60), mRNA.

Price & Availability↑

CloneID	OFa05767
Clone ID Related Accession (Same CDS sequence)	NM_142877.1 , NM_001275938.1
Accession Version	NM_142877.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rad60, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGACG ACGACTGCGA TATATTCAGT GCTGCTCGCA AGCGGATTCC CGAAATAGCT 
TTGCCCAAGA ATCCTTACAA TCTTGGCAAT GATTCCTTCT CCAAGGAGGT GGACTACGAT 
TTCATTGAAG ACCTCCCCAA GAAATCAACT AAAACTGGCA AACGAAAGAA CCCTGCAGCG 
ACGCGGAAAA ATCCACCCAA AAGTCAGGAT GATGCTTCGC CACCAAAACA GGCAGAACAT 
AAGGCTGTGG AGCCAGAAGA GGATATGAGA ACAGAGCGCT CCCTATCACC GGTATCCCTG 
TTGATATTGG AGATGGAAAA GAAAAATGGC CAGCAATCGG ATGTGGAGAA GCACGCCAAA 
GAAAATGACG TTGGTCCTGT GGCCAGACGC ACACGGTCAT CGTTAAATAG GTCAGAAATG 
GCGCCACCAC CTGTTTCACC GACTGTGGAG GTGCCCACAC AGACGAAACC AAAGAAGCGC 
GGCCAAAAAA AGAGAACATC TCTAACAACC ACAACCAGCA ACTCTGCTTC GATGGAGGTC 
TCATTGCCAA GCATTGTAGG TCAGAACAAT GAGCACATTA GCAGACGGTG GACGTTGGCC 
GAAGTTGCAG CCCGTTCCAA AGTGGTGGAC AGTATTGATC TGGTATCTGC GGTCGCTCCC 
CGAGTGGAGG GATTTGTTAA TCTAGACTCT GAAGACGAGG GTGAAAAGGA GGCTCCTCCT 
GTCGTAGAGG AAGAAAATAT TTTTGATAAC GATAATCCCA CAATAGAAGT AGCTTTGTCG 
TGGCTTGGCG AGATCCAGAT TTATAAATTG CGGCAGCACC AAAAGTTTAA GCATCTGTTC 
AAGGAGTTAG CATCACGCAA TGGGATAGAT GAGAATGATA TAACGGTGGA TATGTACTAT 
AACTTCGTTG GACCTGAGGA TACGCCGCAC AGCATTGGTC TCAAATCATT TCATACGCTC 
ACTGGCCATC CAACAAAGAG TCACAATAAT AACAACGTGG CTGCTAAAAT TGACTACAAT 
CCAGAAGCTC TTTGCCGGAA ACCTAAGAAG TTTCAAGTTA AAGTACAGGC GGATAAATGG 
AAGCATCCTT TGGTGATACC CATGAAGAAA ACTGATAATT TCAAAATCAT ATTTATTAAG 
TGTGCTGAGG AGTTGAACTG TGATCCCCGA ACCATTAAAT TGTTTTTTGA CGGTGACTTG 
TTGGATCCAA ATGATACGCC CAATAACCAG GATATGGAGG GCAACGAAGT TATAGATCTT 
AAAATAAAGG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651134.1
CDS	254..1528
Translation	MSDDDCDIFSAARKRIPEIALPKNPYNLGNDSFSKEVDYDFIEDLPKKSTKTGKRKNPAATRKNPPKSQDDASPPKQAEHKAVEPEEDMRTERSLSPVSLLILEMEKKNGQQSDVEKHAKENDVGPVARRTRSSLNRSEMAPPPVSPTVEVPTQTKPKKRGQKKRTSLTTTTSNSASMEVSLPSIVGQNNEHISRRWTLAEVAARSKVVDSIDLVSAVAPRVEGFVNLDSEDEGEKEAPPVVEEENIFDNDNPTIEVALSWLGEIQIYKLRQHQKFKHLFKELASRNGIDENDITVDMYYNFVGPEDTPHSIGLKSFHTLTGHPTKSHNNNNVAAKIDYNPEALCRKPKKFQVKVQADKWKHPLVIPMKKTDNFKIIFIKCAEELNCDPRTIKLFFDGDLLDPNDTPNNQDMEGNEVIDLKIKA

Target ORF information:

RefSeq Version	NM_142877.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rad60, transcript variant A (Rad60), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142877.1

ORF Insert Sequence:

ATGTCCGACG ACGACTGCGA TATATTCAGT GCTGCTCGCA AGCGGATTCC CGAAATAGCT 
TTGCCCAAGA ATCCTTACAA TCTTGGCAAT GATTCCTTCT CCAAGGAGGT GGACTACGAT 
TTCATTGAAG ACCTCCCCAA GAAATCAACT AAAACTGGCA AACGAAAGAA CCCTGCAGCG 
ACGCGGAAAA ATCCACCCAA AAGTCAGGAT GATGCTTCGC CACCAAAACA GGCAGAACAT 
AAGGCTGTGG AGCCAGAAGA GGATATGAGA ACAGAGCGCT CCCTATCACC GGTATCCCTG 
TTGATATTGG AGATGGAAAA GAAAAATGGC CAGCAATCGG ATGTGGAGAA GCACGCCAAA 
GAAAATGACG TTGGTCCTGT GGCCAGACGC ACACGGTCAT CGTTAAATAG GTCAGAAATG 
GCGCCACCAC CTGTTTCACC GACTGTGGAG GTGCCCACAC AGACGAAACC AAAGAAGCGC 
GGCCAAAAAA AGAGAACATC TCTAACAACC ACAACCAGCA ACTCTGCTTC GATGGAGGTC 
TCATTGCCAA GCATTGTAGG TCAGAACAAT GAGCACATTA GCAGACGGTG GACGTTGGCC 
GAAGTTGCAG CCCGTTCCAA AGTGGTGGAC AGTATTGATC TGGTATCTGC GGTCGCTCCC 
CGAGTGGAGG GATTTGTTAA TCTAGACTCT GAAGACGAGG GTGAAAAGGA GGCTCCTCCT 
GTCGTAGAGG AAGAAAATAT TTTTGATAAC GATAATCCCA CAATAGAAGT AGCTTTGTCG 
TGGCTTGGCG AGATCCAGAT TTATAAATTG CGGCAGCACC AAAAGTTTAA GCATCTGTTC 
AAGGAGTTAG CATCACGCAA TGGGATAGAT GAGAATGATA TAACGGTGGA TATGTACTAT 
AACTTCGTTG GACCTGAGGA TACGCCGCAC AGCATTGGTC TCAAATCATT TCATACGCTC 
ACTGGCCATC CAACAAAGAG TCACAATAAT AACAACGTGG CTGCTAAAAT TGACTACAAT 
CCAGAAGCTC TTTGCCGGAA ACCTAAGAAG TTTCAAGTTA AAGTACAGGC GGATAAATGG 
AAGCATCCTT TGGTGATACC CATGAAGAAA ACTGATAATT TCAAAATCAT ATTTATTAAG 
TGTGCTGAGG AGTTGAACTG TGATCCCCGA ACCATTAAAT TGTTTTTTGA CGGTGACTTG 
TTGGATCCAA ATGATACGCC CAATAACCAG GATATGGAGG GCAACGAAGT TATAGATCTT 
AAAATAAAGG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rad60 ( NM_001275938.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262867.1 Drosophila melanogaster Rad60, transcript variant D (Rad60), mRNA.

Price & Availability↑

CloneID	OFa05767
Clone ID Related Accession (Same CDS sequence)	NM_142877.1 , NM_001275938.1
Accession Version	NM_001275938.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rad60, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGACG ACGACTGCGA TATATTCAGT GCTGCTCGCA AGCGGATTCC CGAAATAGCT 
TTGCCCAAGA ATCCTTACAA TCTTGGCAAT GATTCCTTCT CCAAGGAGGT GGACTACGAT 
TTCATTGAAG ACCTCCCCAA GAAATCAACT AAAACTGGCA AACGAAAGAA CCCTGCAGCG 
ACGCGGAAAA ATCCACCCAA AAGTCAGGAT GATGCTTCGC CACCAAAACA GGCAGAACAT 
AAGGCTGTGG AGCCAGAAGA GGATATGAGA ACAGAGCGCT CCCTATCACC GGTATCCCTG 
TTGATATTGG AGATGGAAAA GAAAAATGGC CAGCAATCGG ATGTGGAGAA GCACGCCAAA 
GAAAATGACG TTGGTCCTGT GGCCAGACGC ACACGGTCAT CGTTAAATAG GTCAGAAATG 
GCGCCACCAC CTGTTTCACC GACTGTGGAG GTGCCCACAC AGACGAAACC AAAGAAGCGC 
GGCCAAAAAA AGAGAACATC TCTAACAACC ACAACCAGCA ACTCTGCTTC GATGGAGGTC 
TCATTGCCAA GCATTGTAGG TCAGAACAAT GAGCACATTA GCAGACGGTG GACGTTGGCC 
GAAGTTGCAG CCCGTTCCAA AGTGGTGGAC AGTATTGATC TGGTATCTGC GGTCGCTCCC 
CGAGTGGAGG GATTTGTTAA TCTAGACTCT GAAGACGAGG GTGAAAAGGA GGCTCCTCCT 
GTCGTAGAGG AAGAAAATAT TTTTGATAAC GATAATCCCA CAATAGAAGT AGCTTTGTCG 
TGGCTTGGCG AGATCCAGAT TTATAAATTG CGGCAGCACC AAAAGTTTAA GCATCTGTTC 
AAGGAGTTAG CATCACGCAA TGGGATAGAT GAGAATGATA TAACGGTGGA TATGTACTAT 
AACTTCGTTG GACCTGAGGA TACGCCGCAC AGCATTGGTC TCAAATCATT TCATACGCTC 
ACTGGCCATC CAACAAAGAG TCACAATAAT AACAACGTGG CTGCTAAAAT TGACTACAAT 
CCAGAAGCTC TTTGCCGGAA ACCTAAGAAG TTTCAAGTTA AAGTACAGGC GGATAAATGG 
AAGCATCCTT TGGTGATACC CATGAAGAAA ACTGATAATT TCAAAATCAT ATTTATTAAG 
TGTGCTGAGG AGTTGAACTG TGATCCCCGA ACCATTAAAT TGTTTTTTGA CGGTGACTTG 
TTGGATCCAA ATGATACGCC CAATAACCAG GATATGGAGG GCAACGAAGT TATAGATCTT 
AAAATAAAGG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262867.1
CDS	77..1351
Translation	MSDDDCDIFSAARKRIPEIALPKNPYNLGNDSFSKEVDYDFIEDLPKKSTKTGKRKNPAATRKNPPKSQDDASPPKQAEHKAVEPEEDMRTERSLSPVSLLILEMEKKNGQQSDVEKHAKENDVGPVARRTRSSLNRSEMAPPPVSPTVEVPTQTKPKKRGQKKRTSLTTTTSNSASMEVSLPSIVGQNNEHISRRWTLAEVAARSKVVDSIDLVSAVAPRVEGFVNLDSEDEGEKEAPPVVEEENIFDNDNPTIEVALSWLGEIQIYKLRQHQKFKHLFKELASRNGIDENDITVDMYYNFVGPEDTPHSIGLKSFHTLTGHPTKSHNNNNVAAKIDYNPEALCRKPKKFQVKVQADKWKHPLVIPMKKTDNFKIIFIKCAEELNCDPRTIKLFFDGDLLDPNDTPNNQDMEGNEVIDLKIKA

Target ORF information:

RefSeq Version	NM_001275938.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rad60, transcript variant D (Rad60), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275938.1

ORF Insert Sequence:

ATGTCCGACG ACGACTGCGA TATATTCAGT GCTGCTCGCA AGCGGATTCC CGAAATAGCT 
TTGCCCAAGA ATCCTTACAA TCTTGGCAAT GATTCCTTCT CCAAGGAGGT GGACTACGAT 
TTCATTGAAG ACCTCCCCAA GAAATCAACT AAAACTGGCA AACGAAAGAA CCCTGCAGCG 
ACGCGGAAAA ATCCACCCAA AAGTCAGGAT GATGCTTCGC CACCAAAACA GGCAGAACAT 
AAGGCTGTGG AGCCAGAAGA GGATATGAGA ACAGAGCGCT CCCTATCACC GGTATCCCTG 
TTGATATTGG AGATGGAAAA GAAAAATGGC CAGCAATCGG ATGTGGAGAA GCACGCCAAA 
GAAAATGACG TTGGTCCTGT GGCCAGACGC ACACGGTCAT CGTTAAATAG GTCAGAAATG 
GCGCCACCAC CTGTTTCACC GACTGTGGAG GTGCCCACAC AGACGAAACC AAAGAAGCGC 
GGCCAAAAAA AGAGAACATC TCTAACAACC ACAACCAGCA ACTCTGCTTC GATGGAGGTC 
TCATTGCCAA GCATTGTAGG TCAGAACAAT GAGCACATTA GCAGACGGTG GACGTTGGCC 
GAAGTTGCAG CCCGTTCCAA AGTGGTGGAC AGTATTGATC TGGTATCTGC GGTCGCTCCC 
CGAGTGGAGG GATTTGTTAA TCTAGACTCT GAAGACGAGG GTGAAAAGGA GGCTCCTCCT 
GTCGTAGAGG AAGAAAATAT TTTTGATAAC GATAATCCCA CAATAGAAGT AGCTTTGTCG 
TGGCTTGGCG AGATCCAGAT TTATAAATTG CGGCAGCACC AAAAGTTTAA GCATCTGTTC 
AAGGAGTTAG CATCACGCAA TGGGATAGAT GAGAATGATA TAACGGTGGA TATGTACTAT 
AACTTCGTTG GACCTGAGGA TACGCCGCAC AGCATTGGTC TCAAATCATT TCATACGCTC 
ACTGGCCATC CAACAAAGAG TCACAATAAT AACAACGTGG CTGCTAAAAT TGACTACAAT 
CCAGAAGCTC TTTGCCGGAA ACCTAAGAAG TTTCAAGTTA AAGTACAGGC GGATAAATGG 
AAGCATCCTT TGGTGATACC CATGAAGAAA ACTGATAATT TCAAAATCAT ATTTATTAAG 
TGTGCTGAGG AGTTGAACTG TGATCCCCGA ACCATTAAAT TGTTTTTTGA CGGTGACTTG 
TTGGATCCAA ATGATACGCC CAATAACCAG GATATGGAGG GCAACGAAGT TATAGATCTT 
AAAATAAAGG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rad60 ( NM_001170227.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163698.1 Drosophila melanogaster Rad60, transcript variant B (Rad60), mRNA.

Price & Availability↑

CloneID	OFa07598
Clone ID Related Accession (Same CDS sequence)	NM_001170227.1 , NM_001275937.1
Accession Version	NM_001170227.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rad60, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGACG ACGACTGCGA TATATTCAGT GCTGCTCGCA AGCGGATTCC CGAAATAGCT 
TTGCCCAAGA ATCCTTACAA TCTTGGCAAT GATTCCTTCT CCAAGGAGGT GGACTACGAT 
TTCATTGAAG ACCTCCCCAA GAAATCAACT AAAACTGGCA AACGAAAGAA CCCTGCAGCG 
ACGCGGAAAA ATCCACCCAA AAGTCAGGAT GATGCTTCGC CACCAAAACA GGCAGAACAT 
AAGGCTGTGG AGCCAGAAGA GGATATGAGA ACAGAGCGCT CCCTATCACC GGTATCCCTG 
TTGATATTGG AGATGGAAAA GAAAAATGGC CAGCAATCGG ATGTGGAGAA GCACGCCAAA 
GAAAATGACG TTGGTCCTGT GGCCAGACGC ACACGGTCAT CGTTAAATAG GTCAGAAATG 
GCGCCACCAC CTGTTTCACC GACTGTGGAG GTGCCCACAC AGACGAAACC AAAGAAGCGC 
GGCCAAAAAA AGAGAACATC TCTAACAACC ACAACCAGCA ACTCTGCTTC GATGGAGGTC 
TCATTGCCAA GCATTCAGAA CAATGAGCAC ATTAGCAGAC GGTGGACGTT GGCCGAAGTT 
GCAGCCCGTT CCAAAGTGGT GGACAGTATT GATCTGGTAT CTGCGGTCGC TCCCCGAGTG 
GAGGGATTTG TTAATCTAGA CTCTGAAGAC GAGGGTGAAA AGGAGGCTCC TCCTGTCGTA 
GAGGAAGAAA ATATTTTTGA TAACGATAAT CCCACAATAG AAGTAGCTTT GTCGTGGCTT 
GGCGAGATCC AGATTTATAA ATTGCGGCAG CACCAAAAGT TTAAGCATCT GTTCAAGGAG 
TTAGCATCAC GCAATGGGAT AGATGAGAAT GATATAACGG TGGATATGTA CTATAACTTC 
GTTGGACCTG AGGATACGCC GCACAGCATT GGTCTCAAAT CATTTCATAC GCTCACTGGC 
CATCCAACAA AGAGTCACAA TAATAACAAC GTGGCTGCTA AAATTGACTA CAATCCAGAA 
GCTCTTTGCC GGAAACCTAA GAAGTTTCAA GTTAAAGTAC AGGCGGATAA ATGGAAGCAT 
CCTTTGGTGA TACCCATGAA GAAAACTGAT AATTTCAAAA TCATATTTAT TAAGTGTGCT 
GAGGAGTTGA ACTGTGATCC CCGAACCATT AAATTGTTTT TTGACGGTGA CTTGTTGGAT 
CCAAATGATA CGCCCAATAA CCAGGATATG GAGGGCAACG AAGTTATAGA TCTTAAAATA 
AAGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163698.1
CDS	254..1522
Translation	MSDDDCDIFSAARKRIPEIALPKNPYNLGNDSFSKEVDYDFIEDLPKKSTKTGKRKNPAATRKNPPKSQDDASPPKQAEHKAVEPEEDMRTERSLSPVSLLILEMEKKNGQQSDVEKHAKENDVGPVARRTRSSLNRSEMAPPPVSPTVEVPTQTKPKKRGQKKRTSLTTTTSNSASMEVSLPSIQNNEHISRRWTLAEVAARSKVVDSIDLVSAVAPRVEGFVNLDSEDEGEKEAPPVVEEENIFDNDNPTIEVALSWLGEIQIYKLRQHQKFKHLFKELASRNGIDENDITVDMYYNFVGPEDTPHSIGLKSFHTLTGHPTKSHNNNNVAAKIDYNPEALCRKPKKFQVKVQADKWKHPLVIPMKKTDNFKIIFIKCAEELNCDPRTIKLFFDGDLLDPNDTPNNQDMEGNEVIDLKIKA

Target ORF information:

RefSeq Version	NM_001170227.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rad60, transcript variant B (Rad60), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170227.1

ORF Insert Sequence:

ATGTCCGACG ACGACTGCGA TATATTCAGT GCTGCTCGCA AGCGGATTCC CGAAATAGCT 
TTGCCCAAGA ATCCTTACAA TCTTGGCAAT GATTCCTTCT CCAAGGAGGT GGACTACGAT 
TTCATTGAAG ACCTCCCCAA GAAATCAACT AAAACTGGCA AACGAAAGAA CCCTGCAGCG 
ACGCGGAAAA ATCCACCCAA AAGTCAGGAT GATGCTTCGC CACCAAAACA GGCAGAACAT 
AAGGCTGTGG AGCCAGAAGA GGATATGAGA ACAGAGCGCT CCCTATCACC GGTATCCCTG 
TTGATATTGG AGATGGAAAA GAAAAATGGC CAGCAATCGG ATGTGGAGAA GCACGCCAAA 
GAAAATGACG TTGGTCCTGT GGCCAGACGC ACACGGTCAT CGTTAAATAG GTCAGAAATG 
GCGCCACCAC CTGTTTCACC GACTGTGGAG GTGCCCACAC AGACGAAACC AAAGAAGCGC 
GGCCAAAAAA AGAGAACATC TCTAACAACC ACAACCAGCA ACTCTGCTTC GATGGAGGTC 
TCATTGCCAA GCATTCAGAA CAATGAGCAC ATTAGCAGAC GGTGGACGTT GGCCGAAGTT 
GCAGCCCGTT CCAAAGTGGT GGACAGTATT GATCTGGTAT CTGCGGTCGC TCCCCGAGTG 
GAGGGATTTG TTAATCTAGA CTCTGAAGAC GAGGGTGAAA AGGAGGCTCC TCCTGTCGTA 
GAGGAAGAAA ATATTTTTGA TAACGATAAT CCCACAATAG AAGTAGCTTT GTCGTGGCTT 
GGCGAGATCC AGATTTATAA ATTGCGGCAG CACCAAAAGT TTAAGCATCT GTTCAAGGAG 
TTAGCATCAC GCAATGGGAT AGATGAGAAT GATATAACGG TGGATATGTA CTATAACTTC 
GTTGGACCTG AGGATACGCC GCACAGCATT GGTCTCAAAT CATTTCATAC GCTCACTGGC 
CATCCAACAA AGAGTCACAA TAATAACAAC GTGGCTGCTA AAATTGACTA CAATCCAGAA 
GCTCTTTGCC GGAAACCTAA GAAGTTTCAA GTTAAAGTAC AGGCGGATAA ATGGAAGCAT 
CCTTTGGTGA TACCCATGAA GAAAACTGAT AATTTCAAAA TCATATTTAT TAAGTGTGCT 
GAGGAGTTGA ACTGTGATCC CCGAACCATT AAATTGTTTT TTGACGGTGA CTTGTTGGAT 
CCAAATGATA CGCCCAATAA CCAGGATATG GAGGGCAACG AAGTTATAGA TCTTAAAATA 
AAGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rad60 ( NM_001275937.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262866.1 Drosophila melanogaster Rad60, transcript variant C (Rad60), mRNA.

Price & Availability↑

CloneID	OFa07598
Clone ID Related Accession (Same CDS sequence)	NM_001170227.1 , NM_001275937.1
Accession Version	NM_001275937.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rad60, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGACG ACGACTGCGA TATATTCAGT GCTGCTCGCA AGCGGATTCC CGAAATAGCT 
TTGCCCAAGA ATCCTTACAA TCTTGGCAAT GATTCCTTCT CCAAGGAGGT GGACTACGAT 
TTCATTGAAG ACCTCCCCAA GAAATCAACT AAAACTGGCA AACGAAAGAA CCCTGCAGCG 
ACGCGGAAAA ATCCACCCAA AAGTCAGGAT GATGCTTCGC CACCAAAACA GGCAGAACAT 
AAGGCTGTGG AGCCAGAAGA GGATATGAGA ACAGAGCGCT CCCTATCACC GGTATCCCTG 
TTGATATTGG AGATGGAAAA GAAAAATGGC CAGCAATCGG ATGTGGAGAA GCACGCCAAA 
GAAAATGACG TTGGTCCTGT GGCCAGACGC ACACGGTCAT CGTTAAATAG GTCAGAAATG 
GCGCCACCAC CTGTTTCACC GACTGTGGAG GTGCCCACAC AGACGAAACC AAAGAAGCGC 
GGCCAAAAAA AGAGAACATC TCTAACAACC ACAACCAGCA ACTCTGCTTC GATGGAGGTC 
TCATTGCCAA GCATTCAGAA CAATGAGCAC ATTAGCAGAC GGTGGACGTT GGCCGAAGTT 
GCAGCCCGTT CCAAAGTGGT GGACAGTATT GATCTGGTAT CTGCGGTCGC TCCCCGAGTG 
GAGGGATTTG TTAATCTAGA CTCTGAAGAC GAGGGTGAAA AGGAGGCTCC TCCTGTCGTA 
GAGGAAGAAA ATATTTTTGA TAACGATAAT CCCACAATAG AAGTAGCTTT GTCGTGGCTT 
GGCGAGATCC AGATTTATAA ATTGCGGCAG CACCAAAAGT TTAAGCATCT GTTCAAGGAG 
TTAGCATCAC GCAATGGGAT AGATGAGAAT GATATAACGG TGGATATGTA CTATAACTTC 
GTTGGACCTG AGGATACGCC GCACAGCATT GGTCTCAAAT CATTTCATAC GCTCACTGGC 
CATCCAACAA AGAGTCACAA TAATAACAAC GTGGCTGCTA AAATTGACTA CAATCCAGAA 
GCTCTTTGCC GGAAACCTAA GAAGTTTCAA GTTAAAGTAC AGGCGGATAA ATGGAAGCAT 
CCTTTGGTGA TACCCATGAA GAAAACTGAT AATTTCAAAA TCATATTTAT TAAGTGTGCT 
GAGGAGTTGA ACTGTGATCC CCGAACCATT AAATTGTTTT TTGACGGTGA CTTGTTGGAT 
CCAAATGATA CGCCCAATAA CCAGGATATG GAGGGCAACG AAGTTATAGA TCTTAAAATA 
AAGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262866.1
CDS	77..1345
Translation	MSDDDCDIFSAARKRIPEIALPKNPYNLGNDSFSKEVDYDFIEDLPKKSTKTGKRKNPAATRKNPPKSQDDASPPKQAEHKAVEPEEDMRTERSLSPVSLLILEMEKKNGQQSDVEKHAKENDVGPVARRTRSSLNRSEMAPPPVSPTVEVPTQTKPKKRGQKKRTSLTTTTSNSASMEVSLPSIQNNEHISRRWTLAEVAARSKVVDSIDLVSAVAPRVEGFVNLDSEDEGEKEAPPVVEEENIFDNDNPTIEVALSWLGEIQIYKLRQHQKFKHLFKELASRNGIDENDITVDMYYNFVGPEDTPHSIGLKSFHTLTGHPTKSHNNNNVAAKIDYNPEALCRKPKKFQVKVQADKWKHPLVIPMKKTDNFKIIFIKCAEELNCDPRTIKLFFDGDLLDPNDTPNNQDMEGNEVIDLKIKA

Target ORF information:

RefSeq Version	NM_001275937.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rad60, transcript variant C (Rad60), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275937.1

ORF Insert Sequence:

ATGTCCGACG ACGACTGCGA TATATTCAGT GCTGCTCGCA AGCGGATTCC CGAAATAGCT 
TTGCCCAAGA ATCCTTACAA TCTTGGCAAT GATTCCTTCT CCAAGGAGGT GGACTACGAT 
TTCATTGAAG ACCTCCCCAA GAAATCAACT AAAACTGGCA AACGAAAGAA CCCTGCAGCG 
ACGCGGAAAA ATCCACCCAA AAGTCAGGAT GATGCTTCGC CACCAAAACA GGCAGAACAT 
AAGGCTGTGG AGCCAGAAGA GGATATGAGA ACAGAGCGCT CCCTATCACC GGTATCCCTG 
TTGATATTGG AGATGGAAAA GAAAAATGGC CAGCAATCGG ATGTGGAGAA GCACGCCAAA 
GAAAATGACG TTGGTCCTGT GGCCAGACGC ACACGGTCAT CGTTAAATAG GTCAGAAATG 
GCGCCACCAC CTGTTTCACC GACTGTGGAG GTGCCCACAC AGACGAAACC AAAGAAGCGC 
GGCCAAAAAA AGAGAACATC TCTAACAACC ACAACCAGCA ACTCTGCTTC GATGGAGGTC 
TCATTGCCAA GCATTCAGAA CAATGAGCAC ATTAGCAGAC GGTGGACGTT GGCCGAAGTT 
GCAGCCCGTT CCAAAGTGGT GGACAGTATT GATCTGGTAT CTGCGGTCGC TCCCCGAGTG 
GAGGGATTTG TTAATCTAGA CTCTGAAGAC GAGGGTGAAA AGGAGGCTCC TCCTGTCGTA 
GAGGAAGAAA ATATTTTTGA TAACGATAAT CCCACAATAG AAGTAGCTTT GTCGTGGCTT 
GGCGAGATCC AGATTTATAA ATTGCGGCAG CACCAAAAGT TTAAGCATCT GTTCAAGGAG 
TTAGCATCAC GCAATGGGAT AGATGAGAAT GATATAACGG TGGATATGTA CTATAACTTC 
GTTGGACCTG AGGATACGCC GCACAGCATT GGTCTCAAAT CATTTCATAC GCTCACTGGC 
CATCCAACAA AGAGTCACAA TAATAACAAC GTGGCTGCTA AAATTGACTA CAATCCAGAA 
GCTCTTTGCC GGAAACCTAA GAAGTTTCAA GTTAAAGTAC AGGCGGATAA ATGGAAGCAT 
CCTTTGGTGA TACCCATGAA GAAAACTGAT AATTTCAAAA TCATATTTAT TAAGTGTGCT 
GAGGAGTTGA ACTGTGATCC CCGAACCATT AAATTGTTTT TTGACGGTGA CTTGTTGGAT 
CCAAATGATA CGCCCAATAA CCAGGATATG GAGGGCAACG AAGTTATAGA TCTTAAAATA 
AAGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rad60 ( NM_001275940.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262869.1 Drosophila melanogaster Rad60, transcript variant F (Rad60), mRNA.

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CloneID	OFa07599
Clone ID Related Accession (Same CDS sequence)	NM_001275940.1
Accession Version	NM_001275940.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rad60, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGACG ACGACTGCGA TATATTCAGT GCTGCTCGCA AGCGGATTCC CGAAATAGCT 
TTGCCCAAGA ATCCTTACAA TCTTGGCAAT GATTCCTTCT CCAAGGAGGT GGACTACGAT 
TTCATTGAAG ACCTCCCCAA GAAATCAACT AAAACTGGCA AACGAAAGAA CCCTGCAGCG 
ACGCGGAAAA ATCCACCCAA AAGTCAGGAT GATGCTTCGC CACCAAAACA GGCAGAACAT 
AAGGCTGTGG AGCCAGAAGA GGATATGAGA ACAGAGCGCT CCCTATCACC GGTATCCCTG 
TTGATATTGG AGATGGAAAA GAAAAATGGC CAGCAATCGG ATGTGGAGAA GCACGCCAAA 
GAAAATGACG TTGGTCCTGT GGCCAGACGC ACACGGTCAT CGTTAAATAG GTCAGAAATG 
GCGCCACCAC CTGTTTCACC GACTGTGGAG GTGCCCACAC AGACGAAACC AAAGAAGCGC 
GGCCAAAAAA AGAGAACATC TCTAACAACC ACAACCAGCA ACTCTGCTTC GATGGAGGTC 
TCATTGCCAA GCATTAACAA TGAGCACATT AGCAGACGGT GGACGTTGGC CGAAGTTGCA 
GCCCGTTCCA AAGTGGTGGA CAGTATTGAT CTGGTATCTG CGGTCGCTCC CCGAGTGGAG 
GGATTTGTTA ATCTAGACTC TGAAGACGAG GGTGAAAAGG AGGCTCCTCC TGTCGTAGAG 
GAAGAAAATA TTTTTGATAA CGATAATCCC ACAATAGAAG TAGCTTTGTC GTGGCTTGGC 
GAGATCCAGA TTTATAAATT GCGGCAGCAC CAAAAGTTTA AGCATCTGTT CAAGGAGTTA 
GCATCACGCA ATGGGATAGA TGAGAATGAT ATAACGGTGG ATATGTACTA TAACTTCGTT 
GGACCTGAGG ATACGCCGCA CAGCATTGGT CTCAAATCAT TTCATACGCT CACTGGCCAT 
CCAACAAAGA GTCACAATAA TAACAACGTG GCTGCTAAAA TTGACTACAA TCCAGAAGCT 
CTTTGCCGGA AACCTAAGAA GTTTCAAGTT AAAGTACAGG CGGATAAATG GAAGCATCCT 
TTGGTGATAC CCATGAAGAA AACTGATAAT TTCAAAATCA TATTTATTAA GTGTGCTGAG 
GAGTTGAACT GTGATCCCCG AACCATTAAA TTGTTTTTTG ACGGTGACTT GTTGGATCCA 
AATGATACGC CCAATAACCA GGATATGGAG GGCAACGAAG TTATAGATCT TAAAATAAAG 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262869.1
CDS	77..1342
Translation	MSDDDCDIFSAARKRIPEIALPKNPYNLGNDSFSKEVDYDFIEDLPKKSTKTGKRKNPAATRKNPPKSQDDASPPKQAEHKAVEPEEDMRTERSLSPVSLLILEMEKKNGQQSDVEKHAKENDVGPVARRTRSSLNRSEMAPPPVSPTVEVPTQTKPKKRGQKKRTSLTTTTSNSASMEVSLPSINNEHISRRWTLAEVAARSKVVDSIDLVSAVAPRVEGFVNLDSEDEGEKEAPPVVEEENIFDNDNPTIEVALSWLGEIQIYKLRQHQKFKHLFKELASRNGIDENDITVDMYYNFVGPEDTPHSIGLKSFHTLTGHPTKSHNNNNVAAKIDYNPEALCRKPKKFQVKVQADKWKHPLVIPMKKTDNFKIIFIKCAEELNCDPRTIKLFFDGDLLDPNDTPNNQDMEGNEVIDLKIKA

Target ORF information:

RefSeq Version	NM_001275940.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rad60, transcript variant F (Rad60), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275940.1

ORF Insert Sequence:

ATGTCCGACG ACGACTGCGA TATATTCAGT GCTGCTCGCA AGCGGATTCC CGAAATAGCT 
TTGCCCAAGA ATCCTTACAA TCTTGGCAAT GATTCCTTCT CCAAGGAGGT GGACTACGAT 
TTCATTGAAG ACCTCCCCAA GAAATCAACT AAAACTGGCA AACGAAAGAA CCCTGCAGCG 
ACGCGGAAAA ATCCACCCAA AAGTCAGGAT GATGCTTCGC CACCAAAACA GGCAGAACAT 
AAGGCTGTGG AGCCAGAAGA GGATATGAGA ACAGAGCGCT CCCTATCACC GGTATCCCTG 
TTGATATTGG AGATGGAAAA GAAAAATGGC CAGCAATCGG ATGTGGAGAA GCACGCCAAA 
GAAAATGACG TTGGTCCTGT GGCCAGACGC ACACGGTCAT CGTTAAATAG GTCAGAAATG 
GCGCCACCAC CTGTTTCACC GACTGTGGAG GTGCCCACAC AGACGAAACC AAAGAAGCGC 
GGCCAAAAAA AGAGAACATC TCTAACAACC ACAACCAGCA ACTCTGCTTC GATGGAGGTC 
TCATTGCCAA GCATTAACAA TGAGCACATT AGCAGACGGT GGACGTTGGC CGAAGTTGCA 
GCCCGTTCCA AAGTGGTGGA CAGTATTGAT CTGGTATCTG CGGTCGCTCC CCGAGTGGAG 
GGATTTGTTA ATCTAGACTC TGAAGACGAG GGTGAAAAGG AGGCTCCTCC TGTCGTAGAG 
GAAGAAAATA TTTTTGATAA CGATAATCCC ACAATAGAAG TAGCTTTGTC GTGGCTTGGC 
GAGATCCAGA TTTATAAATT GCGGCAGCAC CAAAAGTTTA AGCATCTGTT CAAGGAGTTA 
GCATCACGCA ATGGGATAGA TGAGAATGAT ATAACGGTGG ATATGTACTA TAACTTCGTT 
GGACCTGAGG ATACGCCGCA CAGCATTGGT CTCAAATCAT TTCATACGCT CACTGGCCAT 
CCAACAAAGA GTCACAATAA TAACAACGTG GCTGCTAAAA TTGACTACAA TCCAGAAGCT 
CTTTGCCGGA AACCTAAGAA GTTTCAAGTT AAAGTACAGG CGGATAAATG GAAGCATCCT 
TTGGTGATAC CCATGAAGAA AACTGATAAT TTCAAAATCA TATTTATTAA GTGTGCTGAG 
GAGTTGAACT GTGATCCCCG AACCATTAAA TTGTTTTTTG ACGGTGACTT GTTGGATCCA 
AATGATACGC CCAATAACCA GGATATGGAG GGCAACGAAG TTATAGATCT TAAAATAAAG 
GCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rad60 ( NM_001275939.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262868.1 Drosophila melanogaster Rad60, transcript variant E (Rad60), mRNA.

Price & Availability↑

CloneID	OFa07600
Clone ID Related Accession (Same CDS sequence)	NM_001275939.1
Accession Version	NM_001275939.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Rad60, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCGACG ACGACTGCGA TATATTCAGT GCTGCTCGCA AGCGGATTCC CGAAATAGCT 
TTGCCCAAGA ATCCTTACAA TCTTGGCAAT GATTCCTTCT CCAAGGAGGT GGACTACGAT 
TTCATTGAAG ACCTCCCCAA GAAATCAACT AAAACTGGCA AACGAAAGAA CCCTGCAGCG 
ACGCGGAAAA ATCCACCCAA AAGTCAGGAT GATGCTTCGC CACCAAAACA GGCAGAACAT 
AAGGCTGTGG AGCCAGAAGA GGATATGAGA ACAGAGCGCT CCCTATCACC GGTATCCCTG 
TTGATATTGG AGATGGAAAA GAAAAATGGC CAGCAATCGG ATGTGGAGAA GCACGCCAAA 
GAAAATGACG TTGGTCCTGT GGCCAGACGC ACACGGTCAT CGTTAAATAG GTCAGAAATG 
GCGCCACCAC CTGTTTCACC GACTGTGGAG GTGCCCACAC AGACGAAACC AAAGAAGCGC 
GGCCAAAAAA AGAGAACATC TCTAACAACC ACAACCAGCA ACTCTGCTTC GATGGAGGTC 
TCATTGCCAA GCATTGTAGG TAACAATGAG CACATTAGCA GACGGTGGAC GTTGGCCGAA 
GTTGCAGCCC GTTCCAAAGT GGTGGACAGT ATTGATCTGG TATCTGCGGT CGCTCCCCGA 
GTGGAGGGAT TTGTTAATCT AGACTCTGAA GACGAGGGTG AAAAGGAGGC TCCTCCTGTC 
GTAGAGGAAG AAAATATTTT TGATAACGAT AATCCCACAA TAGAAGTAGC TTTGTCGTGG 
CTTGGCGAGA TCCAGATTTA TAAATTGCGG CAGCACCAAA AGTTTAAGCA TCTGTTCAAG 
GAGTTAGCAT CACGCAATGG GATAGATGAG AATGATATAA CGGTGGATAT GTACTATAAC 
TTCGTTGGAC CTGAGGATAC GCCGCACAGC ATTGGTCTCA AATCATTTCA TACGCTCACT 
GGCCATCCAA CAAAGAGTCA CAATAATAAC AACGTGGCTG CTAAAATTGA CTACAATCCA 
GAAGCTCTTT GCCGGAAACC TAAGAAGTTT CAAGTTAAAG TACAGGCGGA TAAATGGAAG 
CATCCTTTGG TGATACCCAT GAAGAAAACT GATAATTTCA AAATCATATT TATTAAGTGT 
GCTGAGGAGT TGAACTGTGA TCCCCGAACC ATTAAATTGT TTTTTGACGG TGACTTGTTG 
GATCCAAATG ATACGCCCAA TAACCAGGAT ATGGAGGGCA ACGAAGTTAT AGATCTTAAA 
ATAAAGGCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262868.1
CDS	77..1348
Translation	MSDDDCDIFSAARKRIPEIALPKNPYNLGNDSFSKEVDYDFIEDLPKKSTKTGKRKNPAATRKNPPKSQDDASPPKQAEHKAVEPEEDMRTERSLSPVSLLILEMEKKNGQQSDVEKHAKENDVGPVARRTRSSLNRSEMAPPPVSPTVEVPTQTKPKKRGQKKRTSLTTTTSNSASMEVSLPSIVGNNEHISRRWTLAEVAARSKVVDSIDLVSAVAPRVEGFVNLDSEDEGEKEAPPVVEEENIFDNDNPTIEVALSWLGEIQIYKLRQHQKFKHLFKELASRNGIDENDITVDMYYNFVGPEDTPHSIGLKSFHTLTGHPTKSHNNNNVAAKIDYNPEALCRKPKKFQVKVQADKWKHPLVIPMKKTDNFKIIFIKCAEELNCDPRTIKLFFDGDLLDPNDTPNNQDMEGNEVIDLKIKA

Target ORF information:

RefSeq Version	NM_001275939.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Rad60, transcript variant E (Rad60), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275939.1

ORF Insert Sequence:

ATGTCCGACG ACGACTGCGA TATATTCAGT GCTGCTCGCA AGCGGATTCC CGAAATAGCT 
TTGCCCAAGA ATCCTTACAA TCTTGGCAAT GATTCCTTCT CCAAGGAGGT GGACTACGAT 
TTCATTGAAG ACCTCCCCAA GAAATCAACT AAAACTGGCA AACGAAAGAA CCCTGCAGCG 
ACGCGGAAAA ATCCACCCAA AAGTCAGGAT GATGCTTCGC CACCAAAACA GGCAGAACAT 
AAGGCTGTGG AGCCAGAAGA GGATATGAGA ACAGAGCGCT CCCTATCACC GGTATCCCTG 
TTGATATTGG AGATGGAAAA GAAAAATGGC CAGCAATCGG ATGTGGAGAA GCACGCCAAA 
GAAAATGACG TTGGTCCTGT GGCCAGACGC ACACGGTCAT CGTTAAATAG GTCAGAAATG 
GCGCCACCAC CTGTTTCACC GACTGTGGAG GTGCCCACAC AGACGAAACC AAAGAAGCGC 
GGCCAAAAAA AGAGAACATC TCTAACAACC ACAACCAGCA ACTCTGCTTC GATGGAGGTC 
TCATTGCCAA GCATTGTAGG TAACAATGAG CACATTAGCA GACGGTGGAC GTTGGCCGAA 
GTTGCAGCCC GTTCCAAAGT GGTGGACAGT ATTGATCTGG TATCTGCGGT CGCTCCCCGA 
GTGGAGGGAT TTGTTAATCT AGACTCTGAA GACGAGGGTG AAAAGGAGGC TCCTCCTGTC 
GTAGAGGAAG AAAATATTTT TGATAACGAT AATCCCACAA TAGAAGTAGC TTTGTCGTGG 
CTTGGCGAGA TCCAGATTTA TAAATTGCGG CAGCACCAAA AGTTTAAGCA TCTGTTCAAG 
GAGTTAGCAT CACGCAATGG GATAGATGAG AATGATATAA CGGTGGATAT GTACTATAAC 
TTCGTTGGAC CTGAGGATAC GCCGCACAGC ATTGGTCTCA AATCATTTCA TACGCTCACT 
GGCCATCCAA CAAAGAGTCA CAATAATAAC AACGTGGCTG CTAAAATTGA CTACAATCCA 
GAAGCTCTTT GCCGGAAACC TAAGAAGTTT CAAGTTAAAG TACAGGCGGA TAAATGGAAG 
CATCCTTTGG TGATACCCAT GAAGAAAACT GATAATTTCA AAATCATATT TATTAAGTGT 
GCTGAGGAGT TGAACTGTGA TCCCCGAACC ATTAAATTGT TTTTTGACGG TGACTTGTTG 
GATCCAAATG ATACGCCCAA TAACCAGGAT ATGGAGGGCA ACGAAGTTAT AGATCTTAAA 
ATAAAGGCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Rad60 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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