Irk1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Irk1
Entrez Gene ID	42742
Full Name	Inwardly rectifying potassium channel 1
Synonyms	CG13836,CG44159,CG6747,DIR,Dir,Dm_3R:100326,Dm_3R:100349,Dmel\CG44159,Dmel_CG13836,Dmel_CG6747,Ir,dKirI,ir,irk1
General protein information	Preferred Names Inwardly rectifying potassium channel 1 Names CG44159 gene product from transcript CG44159-RG CG44159-PB CG44159-PC CG44159-PD CG44159-PE CG44159-PF CG44159-PG CG44159-PH Ir Irk1-PB Irk1-PC Irk1-PD Irk1-PE Irk1-PF Irk1-PG Irk1-PH drosophila inward rectifier inwardly rectifying potassium channel inwardly rectifying potassium channel 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 94E4-94E4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001170226.1	NP_001163697.1	inwardly rectifying potassium channel 1, isoform C
NM_001275932.1	NP_001262861.1	inwardly rectifying potassium channel 1, isoform F
NM_001275933.1	NP_001262862.1	inwardly rectifying potassium channel 1, isoform G
NM_001275934.1	NP_001262863.1	inwardly rectifying potassium channel 1, isoform H
NM_001104414.2	NP_001097884.1	inwardly rectifying potassium channel 1, isoform B
NM_001202341.1	NP_001189270.1	inwardly rectifying potassium channel 1, isoform E
NM_142874.3	NP_651131.2	inwardly rectifying potassium channel 1, isoform D

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Kcnj2	NP_058992.1
Xenopus tropicalis (tropical clawed frog)	kcnj2	XP_004916397.1
	KCNJ2	NP_001028031.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001280	XP_321870.5
Pan troglodytes (chimpanzee)	KCNJ2	XP_523701.2
Mus musculus (house mouse)	Kcnj2	NP_032451.1
Gallus gallus (chicken)	KCNJ2	NP_990701.1
Danio rerio (zebrafish)	kcnj2a	NP_001265731.1
Caenorhabditis elegans (roundworm)	irk-1	NP_509138.2
Homo sapiens (human)	KCNJ2	NP_000882.1
Canis lupus familiaris (dog)	KCNJ2	NP_001003120.1
Bos taurus (cattle)	KCNJ2	NP_776798.1
Drosophila melanogaster (fruit fly)	Ir	NP_651131.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Irk1 and Irk2 play redundant roles in transepithelial ion transport in the Drosophila melanogaster renal tubule and are additive to Na(+)/K(+)-ATPase-dependent pathways
Title: Two inwardly rectifying potassium channels, Irk1 and Irk2, play redundant roles in Drosophila renal tubule function.

Drosophila inward rectifier (Dir) is the first putative inward rectifier to be studied in Drosophila.
Title: Characterization of Dir: a putative potassium inward rectifying channel in Drosophila.

The following Irk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Irk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05758	NM_001104414.2 Latest version!	Drosophila melanogaster inwardly rectifying potassium channel 1, transcript variant B (Irk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05759	NM_142874.3 Latest version!	Drosophila melanogaster inwardly rectifying potassium channel 1, transcript variant D (Irk1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa07592	NM_001275932.1 Latest version!	Drosophila melanogaster inwardly rectifying potassium channel 1, transcript variant F (Irk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa07592	NM_001275933.1 Latest version!	Drosophila melanogaster inwardly rectifying potassium channel 1, transcript variant G (Irk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa07593	NM_001202341.1 Latest version!	Drosophila melanogaster inwardly rectifying potassium channel 1, transcript variant E (Irk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa07594	NM_001275934.1 Latest version!	Drosophila melanogaster inwardly rectifying potassium channel 1, transcript variant H (Irk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa07594	NM_001170226.1 Latest version!	Drosophila melanogaster inwardly rectifying potassium channel 1, transcript variant C (Irk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Irk1 ( NM_001104414.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097884.1 Drosophila melanogaster inwardly rectifying potassium channel 1, transcript variant B (Irk1), mRNA.

Price & Availability↑

CloneID	OFa05758
Clone ID Related Accession (Same CDS sequence)	NM_001104414.2
Accession Version	NM_001104414.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	inwardly rectifying potassium channel 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_001104414.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGAGC TGCGTCGCAG TTTAAGTCGA CTCTCGATGA TCGTCTACAG AGCCACCCAT 
TCCAGCGAAC AGTCGTGGCG GGAGGAGCTT CTCAGGTACC GGCAGACACG TTTCAGTTCG 
CGGCGTGTTC GAAAACGTGT GGTCTTCAAG CACGGCGAGT GCAACGTTGT GCAGGGAAAT 
GTGGCCAAGA GACGGCGTCG CTATCTGCAG GACATCTTCA CCACCCTGGT TGACGCCCAG 
TGGCGCTGGA CGCTGCTCGT CTTCGCCGCC AGCTTCGTGT TCTCGTGGGC CTTCTTTGGA 
TTCATCTGGT GGATCATCGC CTACGCACAC AATGATCTGG AGTACACCAA TCTCAAGAAC 
CAGTCACCCG ATCTGGTGGC CAACATAACG CACACGGTCT GCGTAACACA GGTCTCCAAT 
ATGATGTCCG CCTTCCTGTA CTCCGTGGAA ACCCAGACGA CGATAGGCTA TGGTAATCGC 
TATGTGACGG AGGAGTGCCC GGAGGCGATA TTCACGATGT GCATCCAGTG CATCACGGGT 
GTCTTCATCC AGGCGTTCAT GGTGGGCATT GTGTTTGCCA AGTTGTCGCG TCCCAAGAAA 
CGTGCCCAGA CGCTGCTCTT CTCGCGCAAT GCTGTTATCT GCCACCGGGA TGGAGTTCCA 
TGCCTGATGT TCCGTGTGGG AGACATGCGC AAGTCGCACA TCATCGAGGC CCATGTGCGG 
GCCCAGATCA TCCGCAAGAA GGTGACAAAG GAGGGCGAGG TGCTGCCCTT CTACCAGCAG 
GAGTTGCACA TCGGAGCCGA TGGTGGAGAG GATCGGCTAA TGTTCATCTG GCCCACTACC 
ATAGTGCACA AAATTGATAG GAACAGTCCA CTGTACATGC TCTCCGCCTC CGATATGCTA 
AAGGAACGCT TCGAAGTGGT GGTTATGCTG GAGGGCGTCA TCGAGTCCAC TGGCATGACC 
ACACAGGCCA GAAGCAGCTA CTTGCCCTCG GAGGTGCTGT GGGGCCATCG TTTCGTGAAT 
GTGGTGTCCT TCCGCAAGGA GACCGGCGAA TACGAGGTGG ACTACACGCT ATTCAACAAC 
ACCTACGACG TGGACACTCC GCTGTGCAGC GCCAAGCAGC TGGACGAGCT TAAGTCGGAG 
TACTCGAAGA GCGCCAAGTG TGTGAATGCA CCCTTTGCGG ATCGCACGCT CTCGGCCAGC 
TTGTTCCAGC GTATTGCCTC CGCCGCCTCG GTGGATCATC TGGATCCGGC TAGCGACGAA 
TCGCTGGACT CTGGCCGCCT GCAGATACGC TCCCATTCCA TACCCAACGG CGTGCTGGCC 
AGCGAGCTGG AGCCGCTGAA TAACAACAAG CATGGCGCTT CGTTCACCAT GGGTGGCCTG 
ACCATCACGA CGACGGCGCC CAGCATCCCC AACCTATCCA GTATTAACGA GAAGACCAAC 
TCCGGAAATT CCATAAACAG CAGCAATTAC AGCAATAACA ATAGCCTGAG CACGCTGACC 
GGAGGAGGAA GTGCTGCCAG CGGACCCGGA GGAGGCATCA CCATTGTGGC CGGTTCCGGG 
AACCATGGCG GCGCTGGTGG AGGAGGAGGA GGCGGCGGTA GACACAAGTC GAATCCCCGC 
CGTCTTAGCA TCAAGCAGGA TCAGCTGCCC ATCGATTCCA TTTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097884.1
CDS	463..2130
Translation	MSELRRSLSRLSMIVYRATHSSEQSWREELLRYRQTRFSSRRVRKRVVFKHGECNVVQGNVAKRRRRYLQDIFTTLVDAQWRWTLLVFAASFVFSWAFFGFIWWIIAYAHNDLEYTNLKNQSPDLVANITHTVCVTQVSNMMSAFLYSVETQTTIGYGNRYVTEECPEAIFTMCIQCITGVFIQAFMVGIVFAKLSRPKKRAQTLLFSRNAVICHRDGVPCLMFRVGDMRKSHIIEAHVRAQIIRKKVTKEGEVLPFYQQELHIGADGGEDRLMFIWPTTIVHKIDRNSPLYMLSASDMLKERFEVVVMLEGVIESTGMTTQARSSYLPSEVLWGHRFVNVVSFRKETGEYEVDYTLFNNTYDVDTPLCSAKQLDELKSEYSKSAKCVNAPFADRTLSASLFQRIASAASVDHLDPASDESLDSGRLQIRSHSIPNGVLASELEPLNNNKHGASFTMGGLTITTTAPSIPNLSSINEKTNSGNSINSSNYSNNNSLSTLTGGGSAASGPGGGITIVAGSGNHGGAGGGGGGGGRHKSNPRRLSIKQDQLPIDSIC

Target ORF information:

RefSeq Version	NM_001104414.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster inwardly rectifying potassium channel 1, transcript variant B (Irk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104414.2

ORF Insert Sequence:

ATGTCTGAGC TGCGTCGCAG TTTAAGTCGA CTCTCGATGA TCGTCTACAG AGCCACCCAT 
TCCAGCGAAC AGTCGTGGCG GGAGGAGCTT CTCAGGTACC GGCAGACACG TTTCAGTTCG 
CGGCGTGTTC GAAAACGTGT GGTCTTCAAG CACGGCGAGT GCAACGTTGT GCAGGGAAAT 
GTGGCCAAGA GACGGCGTCG CTATCTGCAG GACATCTTCA CCACCCTGGT TGACGCCCAG 
TGGCGCTGGA CGCTGCTCGT CTTCGCCGCC AGCTTCGTGT TCTCGTGGGC CTTCTTTGGA 
TTCATCTGGT GGATCATCGC CTACGCACAC AATGATCTGG AGTACACCAA TCTCAAGAAC 
CAGTCACCCG ATCTGGTGGC CAACATAACG CACACGGTCT GCGTAACACA GGTCTCCAAT 
ATGATGTCCG CCTTCCTGTA CTCCGTGGAA ACCCAGACGA CGATAGGCTA TGGTAATCGC 
TATGTGACGG AGGAGTGCCC GGAGGCGATA TTCACGATGT GCATCCAGTG CATCACGGGT 
GTCTTCATCC AGGCGTTCAT GGTGGGCATT GTGTTTGCCA AGTTGTCGCG TCCCAAGAAA 
CGTGCCCAGA CGCTGCTCTT CTCGCGCAAT GCTGTTATCT GCCACCGGGA TGGAGTTCCA 
TGCCTGATGT TCCGTGTGGG AGACATGCGC AAGTCGCACA TCATCGAGGC CCATGTGCGG 
GCCCAGATCA TCCGCAAGAA GGTGACAAAG GAGGGCGAGG TGCTGCCCTT CTACCAGCAG 
GAGTTGCACA TCGGAGCCGA TGGTGGAGAG GATCGGCTAA TGTTCATCTG GCCCACTACC 
ATAGTGCACA AAATTGATAG GAACAGTCCA CTGTACATGC TCTCCGCCTC CGATATGCTA 
AAGGAACGCT TCGAAGTGGT GGTTATGCTG GAGGGCGTCA TCGAGTCCAC TGGCATGACC 
ACACAGGCCA GAAGCAGCTA CTTGCCCTCG GAGGTGCTGT GGGGCCATCG TTTCGTGAAT 
GTGGTGTCCT TCCGCAAGGA GACCGGCGAA TACGAGGTGG ACTACACGCT ATTCAACAAC 
ACCTACGACG TGGACACTCC GCTGTGCAGC GCCAAGCAGC TGGACGAGCT TAAGTCGGAG 
TACTCGAAGA GCGCCAAGTG TGTGAATGCA CCCTTTGCGG ATCGCACGCT CTCGGCCAGC 
TTGTTCCAGC GTATTGCCTC CGCCGCCTCG GTGGATCATC TGGATCCGGC TAGCGACGAA 
TCGCTGGACT CTGGCCGCCT GCAGATACGC TCCCATTCCA TACCCAACGG CGTGCTGGCC 
AGCGAGCTGG AGCCGCTGAA TAACAACAAG CATGGCGCTT CGTTCACCAT GGGTGGCCTG 
ACCATCACGA CGACGGCGCC CAGCATCCCC AACCTATCCA GTATTAACGA GAAGACCAAC 
TCCGGAAATT CCATAAACAG CAGCAATTAC AGCAATAACA ATAGCCTGAG CACGCTGACC 
GGAGGAGGAA GTGCTGCCAG CGGACCCGGA GGAGGCATCA CCATTGTGGC CGGTTCCGGG 
AACCATGGCG GCGCTGGTGG AGGAGGAGGA GGCGGCGGTA GACACAAGTC GAATCCCCGC 
CGTCTTAGCA TCAAGCAGGA TCAGCTGCCC ATCGATTCCA TTTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Irk1 ( NM_142874.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651131.2 Drosophila melanogaster inwardly rectifying potassium channel 1, transcript variant D (Irk1), partial mRNA.

Price & Availability↑

CloneID	OFa05759
Clone ID Related Accession (Same CDS sequence)	NM_142874.3
Accession Version	NM_142874.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	inwardly rectifying potassium channel 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_142874.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGTACATCT TGTTGTTCCG TTTCAGGTAC CGGCAGACAC GTTTCAGTTC GCGGCGTGTT 
CGAAAACGTG TGGTCTTCAA GCACGGCGAG TGCAACGTTG TGCAGGGAAA TGTGGCCAAG 
AGACGGCGTC GCTATCTGCA GGACATCTTC ACCACCCTGG TTGACGCCCA GTGGCGCTGG 
ACGCTGCTCG TCTTCGCCGC CAGCTTCGTG TTCTCGTGGG CCTTCTTTGG ATTCATCTGG 
TGGATCATCG CCTACGCACA CAATGATCTG GAGTACACCA ATCTCAAGAA CCAGTCACCC 
GATCTGGTGG CCAACATAAC GCACACGGTC TGCGTAACAC AGGTCTCCAA TATGATGTCC 
GCCTTCCTGT ACTCCGTGGA AACCCAGACG ACGATAGGCT ATGGTAATCG CTATGTGACG 
GAGGAGTGCC CGGAGGCGAT ATTCACGATG TGCATCCAGT GCATCACGGG TGTCTTCATC 
CAGGCGTTCA TGGTGGGCAT TGTGTTTGCC AAGTTGTCGC GTCCCAAGAA ACGTGCCCAG 
ACGCTGCTCT TCTCGCGCAA TGCTGTTATC TGCCACCGGG ATGGAGTTCC ATGCCTGATG 
TTCCGTGTGG GAGACATGCG CAAGTCGCAC ATCATCGAGG CCCATGTGCG GGCCCAGATC 
ATCCGCAAGA AGGTGACAAA GGAGGGCGAG GTGCTGCCCT TCTACCAGCA GGAGTTGCAC 
ATCGGAGCCG ATGGTGGAGA GGATCGGCTA ATGTTCATCT GGCCCACTAC CATAGTGCAC 
AAAATTGATA GGAACAGTCC ACTGTACATG CTCTCCGCCT CCGATATGCT AAAGGAACGC 
TTCGAAGTGG TGGTTATGCT GGAGGGCGTC ATCGAGTCCA CTGGCATGAC CACACAGGCC 
AGAAGCAGCT ACTTGCCCTC GGAGGTGCTG TGGGGCCATC GTTTCGTGAA TGTGGTGTCC 
TTCCGCAAGG AGACCGGCGA ATACGAGGTG GACTACACGC TATTCAACAA CACCTACGAC 
GTGGACACTC CGCTGTGCAG CGCCAAGCAG CTGGACGAGC TTAAGTCGGA GTACTCGAAG 
AGCGCCAAGT GTGTGAATGC ACCCTTTGCG GATCGCACGC TCTCGGCCAG CTTGTTCCAG 
CGTATTGCCT CCGCCGCCTC GGTGGATCAT CTGGATCCGG CTAGCGACGA ATCGCTGGAC 
TCTGGCCGCC TGCAGATACG CTCCCATTCC ATACCCAACG GCGTGCTGGC CAGCGAGCTG 
GAGCCGCTGA ATAACAACAA GCATGGCGCT TCGTTCACCA TGGGTGGCCT GACCATCACG 
ACGACGGCGC CCAGCATCCC CAACCTATCC AGTATTAACG AGAAGACCAA CTCCGGAAAT 
TCCATAAACA GCAGCAATTA CAGCAATAAC AATAGCCTGA GCACGCTGAC CGGAGGAGGA 
AGTGCTGCCA GCGGACCCGG AGGAGGCATC ACCATTGTGG CCGGTTCCGG GAACCATGGC 
GGCGCTGGTG GAGGAGGAGG AGGCGGCGGT AGACACAAGT CGAATCCCCG CCGTCTTAGC 
ATCAAGCAGG ATCAGCTGCC CATCGATTCC ATTTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651131.2
CDS	1..1599
Translation	MYILLFRFRYRQTRFSSRRVRKRVVFKHGECNVVQGNVAKRRRRYLQDIFTTLVDAQWRWTLLVFAASFVFSWAFFGFIWWIIAYAHNDLEYTNLKNQSPDLVANITHTVCVTQVSNMMSAFLYSVETQTTIGYGNRYVTEECPEAIFTMCIQCITGVFIQAFMVGIVFAKLSRPKKRAQTLLFSRNAVICHRDGVPCLMFRVGDMRKSHIIEAHVRAQIIRKKVTKEGEVLPFYQQELHIGADGGEDRLMFIWPTTIVHKIDRNSPLYMLSASDMLKERFEVVVMLEGVIESTGMTTQARSSYLPSEVLWGHRFVNVVSFRKETGEYEVDYTLFNNTYDVDTPLCSAKQLDELKSEYSKSAKCVNAPFADRTLSASLFQRIASAASVDHLDPASDESLDSGRLQIRSHSIPNGVLASELEPLNNNKHGASFTMGGLTITTTAPSIPNLSSINEKTNSGNSINSSNYSNNNSLSTLTGGGSAASGPGGGITIVAGSGNHGGAGGGGGGGGRHKSNPRRLSIKQDQLPIDSIC

Target ORF information:

RefSeq Version	NM_142874.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster inwardly rectifying potassium channel 1, transcript variant D (Irk1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142874.3

ORF Insert Sequence:

ATGTACATCT TGTTGTTCCG TTTCAGGTAC CGGCAGACAC GTTTCAGTTC GCGGCGTGTT 
CGAAAACGTG TGGTCTTCAA GCACGGCGAG TGCAACGTTG TGCAGGGAAA TGTGGCCAAG 
AGACGGCGTC GCTATCTGCA GGACATCTTC ACCACCCTGG TTGACGCCCA GTGGCGCTGG 
ACGCTGCTCG TCTTCGCCGC CAGCTTCGTG TTCTCGTGGG CCTTCTTTGG ATTCATCTGG 
TGGATCATCG CCTACGCACA CAATGATCTG GAGTACACCA ATCTCAAGAA CCAGTCACCC 
GATCTGGTGG CCAACATAAC GCACACGGTC TGCGTAACAC AGGTCTCCAA TATGATGTCC 
GCCTTCCTGT ACTCCGTGGA AACCCAGACG ACGATAGGCT ATGGTAATCG CTATGTGACG 
GAGGAGTGCC CGGAGGCGAT ATTCACGATG TGCATCCAGT GCATCACGGG TGTCTTCATC 
CAGGCGTTCA TGGTGGGCAT TGTGTTTGCC AAGTTGTCGC GTCCCAAGAA ACGTGCCCAG 
ACGCTGCTCT TCTCGCGCAA TGCTGTTATC TGCCACCGGG ATGGAGTTCC ATGCCTGATG 
TTCCGTGTGG GAGACATGCG CAAGTCGCAC ATCATCGAGG CCCATGTGCG GGCCCAGATC 
ATCCGCAAGA AGGTGACAAA GGAGGGCGAG GTGCTGCCCT TCTACCAGCA GGAGTTGCAC 
ATCGGAGCCG ATGGTGGAGA GGATCGGCTA ATGTTCATCT GGCCCACTAC CATAGTGCAC 
AAAATTGATA GGAACAGTCC ACTGTACATG CTCTCCGCCT CCGATATGCT AAAGGAACGC 
TTCGAAGTGG TGGTTATGCT GGAGGGCGTC ATCGAGTCCA CTGGCATGAC CACACAGGCC 
AGAAGCAGCT ACTTGCCCTC GGAGGTGCTG TGGGGCCATC GTTTCGTGAA TGTGGTGTCC 
TTCCGCAAGG AGACCGGCGA ATACGAGGTG GACTACACGC TATTCAACAA CACCTACGAC 
GTGGACACTC CGCTGTGCAG CGCCAAGCAG CTGGACGAGC TTAAGTCGGA GTACTCGAAG 
AGCGCCAAGT GTGTGAATGC ACCCTTTGCG GATCGCACGC TCTCGGCCAG CTTGTTCCAG 
CGTATTGCCT CCGCCGCCTC GGTGGATCAT CTGGATCCGG CTAGCGACGA ATCGCTGGAC 
TCTGGCCGCC TGCAGATACG CTCCCATTCC ATACCCAACG GCGTGCTGGC CAGCGAGCTG 
GAGCCGCTGA ATAACAACAA GCATGGCGCT TCGTTCACCA TGGGTGGCCT GACCATCACG 
ACGACGGCGC CCAGCATCCC CAACCTATCC AGTATTAACG AGAAGACCAA CTCCGGAAAT 
TCCATAAACA GCAGCAATTA CAGCAATAAC AATAGCCTGA GCACGCTGAC CGGAGGAGGA 
AGTGCTGCCA GCGGACCCGG AGGAGGCATC ACCATTGTGG CCGGTTCCGG GAACCATGGC 
GGCGCTGGTG GAGGAGGAGG AGGCGGCGGT AGACACAAGT CGAATCCCCG CCGTCTTAGC 
ATCAAGCAGG ATCAGCTGCC CATCGATTCC ATTTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Irk1 ( NM_001275932.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262861.1 Drosophila melanogaster inwardly rectifying potassium channel 1, transcript variant F (Irk1), mRNA.

Price & Availability↑

CloneID	OFa07592
Clone ID Related Accession (Same CDS sequence)	NM_001275932.1 , NM_001275933.1
Accession Version	NM_001275932.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	inwardly rectifying potassium channel 1, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAAAAA TGATAGTGGA TAAATCGGAA CTGGTGGGCG AGAATGCCTG GGCAAAACTA 
CTGCCCGATG AGAAGGATAA GGCCATCATT GTGAAGATGA ACAAAAGTAG TAGCAATAAC 
AACGATTCCA AGGAGACGAC GCCCCTCAAT GTGGATCAAC TGGTGGCAGC CAGCAATCCA 
GCAGTTGCCG GCGAGCAAAG TGCCGTCTGC CTGGAGGATA CCTACAAGCG TACCTCAACT 
TATGCCTCAA ACATCAATTC AACGGGCGGC GAGTCTAGCG GCAGTGGAAC TCAACTTTCC 
CGCAAGGAGT CCATCTTGGG CAGCTTTCAT CGGCAAATCC GGCGATCCAT AAAAGCGGTT 
AGCGAATCGC CCGGCCCACT TACGAGGTAC CGGCAGACAC GTTTCAGTTC GCGGCGTGTT 
CGAAAACGTG TGGTCTTCAA GCACGGCGAG TGCAACGTTG TGCAGGGAAA TGTGGCCAAG 
AGACGGCGTC GCTATCTGCA GGACATCTTC ACCACCCTGG TTGACGCCCA GTGGCGCTGG 
ACGCTGCTCG TCTTCGCCGC CAGCTTCGTG TTCTCGTGGG CCTTCTTTGG ATTCATCTGG 
TGGATCATCG CCTACGCACA CAATGATCTG GAGTACACCA ATCTCAAGAA CCAGTCACCC 
GATCTGGTGG CCAACATAAC GCACACGGTC TGCGTAACAC AGGTCTCCAA TATGATGTCC 
GCCTTCCTGT ACTCCGTGGA AACCCAGACG ACGATAGGCT ATGGTAATCG CTATGTGACG 
GAGGAGTGCC CGGAGGCGAT ATTCACGATG TGCATCCAGT GCATCACGGG TGTCTTCATC 
CAGGCGTTCA TGGTGGGCAT TGTGTTTGCC AAGTTGTCGC GTCCCAAGAA ACGTGCCCAG 
ACGCTGCTCT TCTCGCGCAA TGCTGTTATC TGCCACCGGG ATGGAGTTCC ATGCCTGATG 
TTCCGTGTGG GAGACATGCG CAAGTCGCAC ATCATCGAGG CCCATGTGCG GGCCCAGATC 
ATCCGCAAGA AGGTGACAAA GGAGGGCGAG GTGCTGCCCT TCTACCAGCA GGAGTTGCAC 
ATCGGAGCCG ATGGTGGAGA GGATCGGCTA ATGTTCATCT GGCCCACTAC CATAGTGCAC 
AAAATTGATA GGAACAGTCC ACTGTACATG CTCTCCGCCT CCGATATGCT AAAGGAACGC 
TTCGAAGTGG TGGTTATGCT GGAGGGCGTC ATCGAGTCCA CTGGCATGAC CACACAGGCC 
AGAAGCAGCT ACTTGCCCTC GGAGGTGCTG TGGGGCCATC GTTTCGTGAA TGTGGTGTCC 
TTCCGCAAGG AGACCGGCGA ATACGAGGTG GACTACACGC TATTCAACAA CACCTACGAC 
GTGGACACTC CGCTGTGCAG CGCCAAGCAG CTGGACGAGC TTAAGTCGGA GTACTCGAAG 
AGCGCCAAGT GTGTGAATGC ACCCTTTGCG GATCGCACGC TCTCGGCCAG CTTGTTCCAG 
CGTATTGCCT CCGCCGCCTC GGTGGATCAT CTGGATCCGG CTAGCGACGA ATCGCTGGAC 
TCTGGCCGCC TGCAGATACG CTCCCATTCC ATACCCAACG GCGTGCTGGC CAGCGAGCTG 
GAGCCGCTGA ATAACAACAA GCATGGCGCT TCGTTCACCA TGGGTGGCCT GACCATCACG 
ACGACGGCGC CCAGCATCCC CAACCTATCC AGTATTAACG AGAAGACCAA CTCCGGAAAT 
TCCATAAACA GCAGCAATTA CAGCAATAAC AATAGCCTGA GCACGCTGAC CGGAGGAGGA 
AGTGCTGCCA GCGGACCCGG AGGAGGCATC ACCATTGTGG CCGGTTCCGG GAACCATGGC 
GGCGCTGGTG GAGGAGGAGG AGGCGGCGGT AGACACAAGT CGAATCCCCG CCGTCTTAGC 
ATCAAGCAGG ATCAGCTGCC CATCGATTCC ATTTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262861.1
CDS	547..2505
Translation	MPKMIVDKSELVGENAWAKLLPDEKDKAIIVKMNKSSSNNNDSKETTPLNVDQLVAASNPAVAGEQSAVCLEDTYKRTSTYASNINSTGGESSGSGTQLSRKESILGSFHRQIRRSIKAVSESPGPLTRYRQTRFSSRRVRKRVVFKHGECNVVQGNVAKRRRRYLQDIFTTLVDAQWRWTLLVFAASFVFSWAFFGFIWWIIAYAHNDLEYTNLKNQSPDLVANITHTVCVTQVSNMMSAFLYSVETQTTIGYGNRYVTEECPEAIFTMCIQCITGVFIQAFMVGIVFAKLSRPKKRAQTLLFSRNAVICHRDGVPCLMFRVGDMRKSHIIEAHVRAQIIRKKVTKEGEVLPFYQQELHIGADGGEDRLMFIWPTTIVHKIDRNSPLYMLSASDMLKERFEVVVMLEGVIESTGMTTQARSSYLPSEVLWGHRFVNVVSFRKETGEYEVDYTLFNNTYDVDTPLCSAKQLDELKSEYSKSAKCVNAPFADRTLSASLFQRIASAASVDHLDPASDESLDSGRLQIRSHSIPNGVLASELEPLNNNKHGASFTMGGLTITTTAPSIPNLSSINEKTNSGNSINSSNYSNNNSLSTLTGGGSAASGPGGGITIVAGSGNHGGAGGGGGGGGRHKSNPRRLSIKQDQLPIDSIC

Target ORF information:

RefSeq Version	NM_001275932.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster inwardly rectifying potassium channel 1, transcript variant F (Irk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275932.1

ORF Insert Sequence:

ATGCCAAAAA TGATAGTGGA TAAATCGGAA CTGGTGGGCG AGAATGCCTG GGCAAAACTA 
CTGCCCGATG AGAAGGATAA GGCCATCATT GTGAAGATGA ACAAAAGTAG TAGCAATAAC 
AACGATTCCA AGGAGACGAC GCCCCTCAAT GTGGATCAAC TGGTGGCAGC CAGCAATCCA 
GCAGTTGCCG GCGAGCAAAG TGCCGTCTGC CTGGAGGATA CCTACAAGCG TACCTCAACT 
TATGCCTCAA ACATCAATTC AACGGGCGGC GAGTCTAGCG GCAGTGGAAC TCAACTTTCC 
CGCAAGGAGT CCATCTTGGG CAGCTTTCAT CGGCAAATCC GGCGATCCAT AAAAGCGGTT 
AGCGAATCGC CCGGCCCACT TACGAGGTAC CGGCAGACAC GTTTCAGTTC GCGGCGTGTT 
CGAAAACGTG TGGTCTTCAA GCACGGCGAG TGCAACGTTG TGCAGGGAAA TGTGGCCAAG 
AGACGGCGTC GCTATCTGCA GGACATCTTC ACCACCCTGG TTGACGCCCA GTGGCGCTGG 
ACGCTGCTCG TCTTCGCCGC CAGCTTCGTG TTCTCGTGGG CCTTCTTTGG ATTCATCTGG 
TGGATCATCG CCTACGCACA CAATGATCTG GAGTACACCA ATCTCAAGAA CCAGTCACCC 
GATCTGGTGG CCAACATAAC GCACACGGTC TGCGTAACAC AGGTCTCCAA TATGATGTCC 
GCCTTCCTGT ACTCCGTGGA AACCCAGACG ACGATAGGCT ATGGTAATCG CTATGTGACG 
GAGGAGTGCC CGGAGGCGAT ATTCACGATG TGCATCCAGT GCATCACGGG TGTCTTCATC 
CAGGCGTTCA TGGTGGGCAT TGTGTTTGCC AAGTTGTCGC GTCCCAAGAA ACGTGCCCAG 
ACGCTGCTCT TCTCGCGCAA TGCTGTTATC TGCCACCGGG ATGGAGTTCC ATGCCTGATG 
TTCCGTGTGG GAGACATGCG CAAGTCGCAC ATCATCGAGG CCCATGTGCG GGCCCAGATC 
ATCCGCAAGA AGGTGACAAA GGAGGGCGAG GTGCTGCCCT TCTACCAGCA GGAGTTGCAC 
ATCGGAGCCG ATGGTGGAGA GGATCGGCTA ATGTTCATCT GGCCCACTAC CATAGTGCAC 
AAAATTGATA GGAACAGTCC ACTGTACATG CTCTCCGCCT CCGATATGCT AAAGGAACGC 
TTCGAAGTGG TGGTTATGCT GGAGGGCGTC ATCGAGTCCA CTGGCATGAC CACACAGGCC 
AGAAGCAGCT ACTTGCCCTC GGAGGTGCTG TGGGGCCATC GTTTCGTGAA TGTGGTGTCC 
TTCCGCAAGG AGACCGGCGA ATACGAGGTG GACTACACGC TATTCAACAA CACCTACGAC 
GTGGACACTC CGCTGTGCAG CGCCAAGCAG CTGGACGAGC TTAAGTCGGA GTACTCGAAG 
AGCGCCAAGT GTGTGAATGC ACCCTTTGCG GATCGCACGC TCTCGGCCAG CTTGTTCCAG 
CGTATTGCCT CCGCCGCCTC GGTGGATCAT CTGGATCCGG CTAGCGACGA ATCGCTGGAC 
TCTGGCCGCC TGCAGATACG CTCCCATTCC ATACCCAACG GCGTGCTGGC CAGCGAGCTG 
GAGCCGCTGA ATAACAACAA GCATGGCGCT TCGTTCACCA TGGGTGGCCT GACCATCACG 
ACGACGGCGC CCAGCATCCC CAACCTATCC AGTATTAACG AGAAGACCAA CTCCGGAAAT 
TCCATAAACA GCAGCAATTA CAGCAATAAC AATAGCCTGA GCACGCTGAC CGGAGGAGGA 
AGTGCTGCCA GCGGACCCGG AGGAGGCATC ACCATTGTGG CCGGTTCCGG GAACCATGGC 
GGCGCTGGTG GAGGAGGAGG AGGCGGCGGT AGACACAAGT CGAATCCCCG CCGTCTTAGC 
ATCAAGCAGG ATCAGCTGCC CATCGATTCC ATTTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Irk1 ( NM_001275933.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262862.1 Drosophila melanogaster inwardly rectifying potassium channel 1, transcript variant G (Irk1), mRNA.

Price & Availability↑

CloneID	OFa07592
Clone ID Related Accession (Same CDS sequence)	NM_001275932.1 , NM_001275933.1
Accession Version	NM_001275933.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	inwardly rectifying potassium channel 1, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCAAAAA TGATAGTGGA TAAATCGGAA CTGGTGGGCG AGAATGCCTG GGCAAAACTA 
CTGCCCGATG AGAAGGATAA GGCCATCATT GTGAAGATGA ACAAAAGTAG TAGCAATAAC 
AACGATTCCA AGGAGACGAC GCCCCTCAAT GTGGATCAAC TGGTGGCAGC CAGCAATCCA 
GCAGTTGCCG GCGAGCAAAG TGCCGTCTGC CTGGAGGATA CCTACAAGCG TACCTCAACT 
TATGCCTCAA ACATCAATTC AACGGGCGGC GAGTCTAGCG GCAGTGGAAC TCAACTTTCC 
CGCAAGGAGT CCATCTTGGG CAGCTTTCAT CGGCAAATCC GGCGATCCAT AAAAGCGGTT 
AGCGAATCGC CCGGCCCACT TACGAGGTAC CGGCAGACAC GTTTCAGTTC GCGGCGTGTT 
CGAAAACGTG TGGTCTTCAA GCACGGCGAG TGCAACGTTG TGCAGGGAAA TGTGGCCAAG 
AGACGGCGTC GCTATCTGCA GGACATCTTC ACCACCCTGG TTGACGCCCA GTGGCGCTGG 
ACGCTGCTCG TCTTCGCCGC CAGCTTCGTG TTCTCGTGGG CCTTCTTTGG ATTCATCTGG 
TGGATCATCG CCTACGCACA CAATGATCTG GAGTACACCA ATCTCAAGAA CCAGTCACCC 
GATCTGGTGG CCAACATAAC GCACACGGTC TGCGTAACAC AGGTCTCCAA TATGATGTCC 
GCCTTCCTGT ACTCCGTGGA AACCCAGACG ACGATAGGCT ATGGTAATCG CTATGTGACG 
GAGGAGTGCC CGGAGGCGAT ATTCACGATG TGCATCCAGT GCATCACGGG TGTCTTCATC 
CAGGCGTTCA TGGTGGGCAT TGTGTTTGCC AAGTTGTCGC GTCCCAAGAA ACGTGCCCAG 
ACGCTGCTCT TCTCGCGCAA TGCTGTTATC TGCCACCGGG ATGGAGTTCC ATGCCTGATG 
TTCCGTGTGG GAGACATGCG CAAGTCGCAC ATCATCGAGG CCCATGTGCG GGCCCAGATC 
ATCCGCAAGA AGGTGACAAA GGAGGGCGAG GTGCTGCCCT TCTACCAGCA GGAGTTGCAC 
ATCGGAGCCG ATGGTGGAGA GGATCGGCTA ATGTTCATCT GGCCCACTAC CATAGTGCAC 
AAAATTGATA GGAACAGTCC ACTGTACATG CTCTCCGCCT CCGATATGCT AAAGGAACGC 
TTCGAAGTGG TGGTTATGCT GGAGGGCGTC ATCGAGTCCA CTGGCATGAC CACACAGGCC 
AGAAGCAGCT ACTTGCCCTC GGAGGTGCTG TGGGGCCATC GTTTCGTGAA TGTGGTGTCC 
TTCCGCAAGG AGACCGGCGA ATACGAGGTG GACTACACGC TATTCAACAA CACCTACGAC 
GTGGACACTC CGCTGTGCAG CGCCAAGCAG CTGGACGAGC TTAAGTCGGA GTACTCGAAG 
AGCGCCAAGT GTGTGAATGC ACCCTTTGCG GATCGCACGC TCTCGGCCAG CTTGTTCCAG 
CGTATTGCCT CCGCCGCCTC GGTGGATCAT CTGGATCCGG CTAGCGACGA ATCGCTGGAC 
TCTGGCCGCC TGCAGATACG CTCCCATTCC ATACCCAACG GCGTGCTGGC CAGCGAGCTG 
GAGCCGCTGA ATAACAACAA GCATGGCGCT TCGTTCACCA TGGGTGGCCT GACCATCACG 
ACGACGGCGC CCAGCATCCC CAACCTATCC AGTATTAACG AGAAGACCAA CTCCGGAAAT 
TCCATAAACA GCAGCAATTA CAGCAATAAC AATAGCCTGA GCACGCTGAC CGGAGGAGGA 
AGTGCTGCCA GCGGACCCGG AGGAGGCATC ACCATTGTGG CCGGTTCCGG GAACCATGGC 
GGCGCTGGTG GAGGAGGAGG AGGCGGCGGT AGACACAAGT CGAATCCCCG CCGTCTTAGC 
ATCAAGCAGG ATCAGCTGCC CATCGATTCC ATTTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262862.1
CDS	269..2227
Translation	MPKMIVDKSELVGENAWAKLLPDEKDKAIIVKMNKSSSNNNDSKETTPLNVDQLVAASNPAVAGEQSAVCLEDTYKRTSTYASNINSTGGESSGSGTQLSRKESILGSFHRQIRRSIKAVSESPGPLTRYRQTRFSSRRVRKRVVFKHGECNVVQGNVAKRRRRYLQDIFTTLVDAQWRWTLLVFAASFVFSWAFFGFIWWIIAYAHNDLEYTNLKNQSPDLVANITHTVCVTQVSNMMSAFLYSVETQTTIGYGNRYVTEECPEAIFTMCIQCITGVFIQAFMVGIVFAKLSRPKKRAQTLLFSRNAVICHRDGVPCLMFRVGDMRKSHIIEAHVRAQIIRKKVTKEGEVLPFYQQELHIGADGGEDRLMFIWPTTIVHKIDRNSPLYMLSASDMLKERFEVVVMLEGVIESTGMTTQARSSYLPSEVLWGHRFVNVVSFRKETGEYEVDYTLFNNTYDVDTPLCSAKQLDELKSEYSKSAKCVNAPFADRTLSASLFQRIASAASVDHLDPASDESLDSGRLQIRSHSIPNGVLASELEPLNNNKHGASFTMGGLTITTTAPSIPNLSSINEKTNSGNSINSSNYSNNNSLSTLTGGGSAASGPGGGITIVAGSGNHGGAGGGGGGGGRHKSNPRRLSIKQDQLPIDSIC

Target ORF information:

RefSeq Version	NM_001275933.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster inwardly rectifying potassium channel 1, transcript variant G (Irk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275933.1

ORF Insert Sequence:

ATGCCAAAAA TGATAGTGGA TAAATCGGAA CTGGTGGGCG AGAATGCCTG GGCAAAACTA 
CTGCCCGATG AGAAGGATAA GGCCATCATT GTGAAGATGA ACAAAAGTAG TAGCAATAAC 
AACGATTCCA AGGAGACGAC GCCCCTCAAT GTGGATCAAC TGGTGGCAGC CAGCAATCCA 
GCAGTTGCCG GCGAGCAAAG TGCCGTCTGC CTGGAGGATA CCTACAAGCG TACCTCAACT 
TATGCCTCAA ACATCAATTC AACGGGCGGC GAGTCTAGCG GCAGTGGAAC TCAACTTTCC 
CGCAAGGAGT CCATCTTGGG CAGCTTTCAT CGGCAAATCC GGCGATCCAT AAAAGCGGTT 
AGCGAATCGC CCGGCCCACT TACGAGGTAC CGGCAGACAC GTTTCAGTTC GCGGCGTGTT 
CGAAAACGTG TGGTCTTCAA GCACGGCGAG TGCAACGTTG TGCAGGGAAA TGTGGCCAAG 
AGACGGCGTC GCTATCTGCA GGACATCTTC ACCACCCTGG TTGACGCCCA GTGGCGCTGG 
ACGCTGCTCG TCTTCGCCGC CAGCTTCGTG TTCTCGTGGG CCTTCTTTGG ATTCATCTGG 
TGGATCATCG CCTACGCACA CAATGATCTG GAGTACACCA ATCTCAAGAA CCAGTCACCC 
GATCTGGTGG CCAACATAAC GCACACGGTC TGCGTAACAC AGGTCTCCAA TATGATGTCC 
GCCTTCCTGT ACTCCGTGGA AACCCAGACG ACGATAGGCT ATGGTAATCG CTATGTGACG 
GAGGAGTGCC CGGAGGCGAT ATTCACGATG TGCATCCAGT GCATCACGGG TGTCTTCATC 
CAGGCGTTCA TGGTGGGCAT TGTGTTTGCC AAGTTGTCGC GTCCCAAGAA ACGTGCCCAG 
ACGCTGCTCT TCTCGCGCAA TGCTGTTATC TGCCACCGGG ATGGAGTTCC ATGCCTGATG 
TTCCGTGTGG GAGACATGCG CAAGTCGCAC ATCATCGAGG CCCATGTGCG GGCCCAGATC 
ATCCGCAAGA AGGTGACAAA GGAGGGCGAG GTGCTGCCCT TCTACCAGCA GGAGTTGCAC 
ATCGGAGCCG ATGGTGGAGA GGATCGGCTA ATGTTCATCT GGCCCACTAC CATAGTGCAC 
AAAATTGATA GGAACAGTCC ACTGTACATG CTCTCCGCCT CCGATATGCT AAAGGAACGC 
TTCGAAGTGG TGGTTATGCT GGAGGGCGTC ATCGAGTCCA CTGGCATGAC CACACAGGCC 
AGAAGCAGCT ACTTGCCCTC GGAGGTGCTG TGGGGCCATC GTTTCGTGAA TGTGGTGTCC 
TTCCGCAAGG AGACCGGCGA ATACGAGGTG GACTACACGC TATTCAACAA CACCTACGAC 
GTGGACACTC CGCTGTGCAG CGCCAAGCAG CTGGACGAGC TTAAGTCGGA GTACTCGAAG 
AGCGCCAAGT GTGTGAATGC ACCCTTTGCG GATCGCACGC TCTCGGCCAG CTTGTTCCAG 
CGTATTGCCT CCGCCGCCTC GGTGGATCAT CTGGATCCGG CTAGCGACGA ATCGCTGGAC 
TCTGGCCGCC TGCAGATACG CTCCCATTCC ATACCCAACG GCGTGCTGGC CAGCGAGCTG 
GAGCCGCTGA ATAACAACAA GCATGGCGCT TCGTTCACCA TGGGTGGCCT GACCATCACG 
ACGACGGCGC CCAGCATCCC CAACCTATCC AGTATTAACG AGAAGACCAA CTCCGGAAAT 
TCCATAAACA GCAGCAATTA CAGCAATAAC AATAGCCTGA GCACGCTGAC CGGAGGAGGA 
AGTGCTGCCA GCGGACCCGG AGGAGGCATC ACCATTGTGG CCGGTTCCGG GAACCATGGC 
GGCGCTGGTG GAGGAGGAGG AGGCGGCGGT AGACACAAGT CGAATCCCCG CCGTCTTAGC 
ATCAAGCAGG ATCAGCTGCC CATCGATTCC ATTTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Irk1 ( NM_001202341.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189270.1 Drosophila melanogaster inwardly rectifying potassium channel 1, transcript variant E (Irk1), mRNA.

Price & Availability↑

CloneID	OFa07593
Clone ID Related Accession (Same CDS sequence)	NM_001202341.1
Accession Version	NM_001202341.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	inwardly rectifying potassium channel 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCGTC TGATGACCTG GGAGCGCGAT CTGGTGGATG CCATGTACGA GTACCGGCAG 
ACACGTTTCA GTTCGCGGCG TGTTCGAAAA CGTGTGGTCT TCAAGCACGG CGAGTGCAAC 
GTTGTGCAGG GAAATGTGGC CAAGAGACGG CGTCGCTATC TGCAGGACAT CTTCACCACC 
CTGGTTGACG CCCAGTGGCG CTGGACGCTG CTCGTCTTCG CCGCCAGCTT CGTGTTCTCG 
TGGGCCTTCT TTGGATTCAT CTGGTGGATC ATCGCCTACG CACACAATGA TCTGGAGTAC 
ACCAATCTCA AGAACCAGTC ACCCGATCTG GTGGCCAACA TAACGCACAC GGTCTGCGTA 
ACACAGGTCT CCAATATGAT GTCCGCCTTC CTGTACTCCG TGGAAACCCA GACGACGATA 
GGCTATGGTA ATCGCTATGT GACGGAGGAG TGCCCGGAGG CGATATTCAC GATGTGCATC 
CAGTGCATCA CGGGTGTCTT CATCCAGGCG TTCATGGTGG GCATTGTGTT TGCCAAGTTG 
TCGCGTCCCA AGAAACGTGC CCAGACGCTG CTCTTCTCGC GCAATGCTGT TATCTGCCAC 
CGGGATGGAG TTCCATGCCT GATGTTCCGT GTGGGAGACA TGCGCAAGTC GCACATCATC 
GAGGCCCATG TGCGGGCCCA GATCATCCGC AAGAAGGTGA CAAAGGAGGG CGAGGTGCTG 
CCCTTCTACC AGCAGGAGTT GCACATCGGA GCCGATGGTG GAGAGGATCG GCTAATGTTC 
ATCTGGCCCA CTACCATAGT GCACAAAATT GATAGGAACA GTCCACTGTA CATGCTCTCC 
GCCTCCGATA TGCTAAAGGA ACGCTTCGAA GTGGTGGTTA TGCTGGAGGG CGTCATCGAG 
TCCACTGGCA TGACCACACA GGCCAGAAGC AGCTACTTGC CCTCGGAGGT GCTGTGGGGC 
CATCGTTTCG TGAATGTGGT GTCCTTCCGC AAGGAGACCG GCGAATACGA GGTGGACTAC 
ACGCTATTCA ACAACACCTA CGACGTGGAC ACTCCGCTGT GCAGCGCCAA GCAGCTGGAC 
GAGCTTAAGT CGGAGTACTC GAAGAGCGCC AAGTGTGTGA ATGCACCCTT TGCGGATCGC 
ACGCTCTCGG CCAGCTTGTT CCAGCGTATT GCCTCCGCCG CCTCGGTGGA TCATCTGGAT 
CCGGCTAGCG ACGAATCGCT GGACTCTGGC CGCCTGCAGA TACGCTCCCA TTCCATACCC 
AACGGCGTGC TGGCCAGCGA GCTGGAGCCG CTGAATAACA ACAAGCATGG CGCTTCGTTC 
ACCATGGGTG GCCTGACCAT CACGACGACG GCGCCCAGCA TCCCCAACCT ATCCAGTATT 
AACGAGAAGA CCAACTCCGG AAATTCCATA AACAGCAGCA ATTACAGCAA TAACAATAGC 
CTGAGCACGC TGACCGGAGG AGGAAGTGCT GCCAGCGGAC CCGGAGGAGG CATCACCATT 
GTGGCCGGTT CCGGGAACCA TGGCGGCGCT GGTGGAGGAG GAGGAGGCGG CGGTAGACAC 
AAGTCGAATC CCCGCCGTCT TAGCATCAAG CAGGATCAGC TGCCCATCGA TTCCATTTGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189270.1
CDS	897..2519
Translation	MKRLMTWERDLVDAMYEYRQTRFSSRRVRKRVVFKHGECNVVQGNVAKRRRRYLQDIFTTLVDAQWRWTLLVFAASFVFSWAFFGFIWWIIAYAHNDLEYTNLKNQSPDLVANITHTVCVTQVSNMMSAFLYSVETQTTIGYGNRYVTEECPEAIFTMCIQCITGVFIQAFMVGIVFAKLSRPKKRAQTLLFSRNAVICHRDGVPCLMFRVGDMRKSHIIEAHVRAQIIRKKVTKEGEVLPFYQQELHIGADGGEDRLMFIWPTTIVHKIDRNSPLYMLSASDMLKERFEVVVMLEGVIESTGMTTQARSSYLPSEVLWGHRFVNVVSFRKETGEYEVDYTLFNNTYDVDTPLCSAKQLDELKSEYSKSAKCVNAPFADRTLSASLFQRIASAASVDHLDPASDESLDSGRLQIRSHSIPNGVLASELEPLNNNKHGASFTMGGLTITTTAPSIPNLSSINEKTNSGNSINSSNYSNNNSLSTLTGGGSAASGPGGGITIVAGSGNHGGAGGGGGGGGRHKSNPRRLSIKQDQLPIDSIC

Target ORF information:

RefSeq Version	NM_001202341.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster inwardly rectifying potassium channel 1, transcript variant E (Irk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202341.1

ORF Insert Sequence:

ATGAAGCGTC TGATGACCTG GGAGCGCGAT CTGGTGGATG CCATGTACGA GTACCGGCAG 
ACACGTTTCA GTTCGCGGCG TGTTCGAAAA CGTGTGGTCT TCAAGCACGG CGAGTGCAAC 
GTTGTGCAGG GAAATGTGGC CAAGAGACGG CGTCGCTATC TGCAGGACAT CTTCACCACC 
CTGGTTGACG CCCAGTGGCG CTGGACGCTG CTCGTCTTCG CCGCCAGCTT CGTGTTCTCG 
TGGGCCTTCT TTGGATTCAT CTGGTGGATC ATCGCCTACG CACACAATGA TCTGGAGTAC 
ACCAATCTCA AGAACCAGTC ACCCGATCTG GTGGCCAACA TAACGCACAC GGTCTGCGTA 
ACACAGGTCT CCAATATGAT GTCCGCCTTC CTGTACTCCG TGGAAACCCA GACGACGATA 
GGCTATGGTA ATCGCTATGT GACGGAGGAG TGCCCGGAGG CGATATTCAC GATGTGCATC 
CAGTGCATCA CGGGTGTCTT CATCCAGGCG TTCATGGTGG GCATTGTGTT TGCCAAGTTG 
TCGCGTCCCA AGAAACGTGC CCAGACGCTG CTCTTCTCGC GCAATGCTGT TATCTGCCAC 
CGGGATGGAG TTCCATGCCT GATGTTCCGT GTGGGAGACA TGCGCAAGTC GCACATCATC 
GAGGCCCATG TGCGGGCCCA GATCATCCGC AAGAAGGTGA CAAAGGAGGG CGAGGTGCTG 
CCCTTCTACC AGCAGGAGTT GCACATCGGA GCCGATGGTG GAGAGGATCG GCTAATGTTC 
ATCTGGCCCA CTACCATAGT GCACAAAATT GATAGGAACA GTCCACTGTA CATGCTCTCC 
GCCTCCGATA TGCTAAAGGA ACGCTTCGAA GTGGTGGTTA TGCTGGAGGG CGTCATCGAG 
TCCACTGGCA TGACCACACA GGCCAGAAGC AGCTACTTGC CCTCGGAGGT GCTGTGGGGC 
CATCGTTTCG TGAATGTGGT GTCCTTCCGC AAGGAGACCG GCGAATACGA GGTGGACTAC 
ACGCTATTCA ACAACACCTA CGACGTGGAC ACTCCGCTGT GCAGCGCCAA GCAGCTGGAC 
GAGCTTAAGT CGGAGTACTC GAAGAGCGCC AAGTGTGTGA ATGCACCCTT TGCGGATCGC 
ACGCTCTCGG CCAGCTTGTT CCAGCGTATT GCCTCCGCCG CCTCGGTGGA TCATCTGGAT 
CCGGCTAGCG ACGAATCGCT GGACTCTGGC CGCCTGCAGA TACGCTCCCA TTCCATACCC 
AACGGCGTGC TGGCCAGCGA GCTGGAGCCG CTGAATAACA ACAAGCATGG CGCTTCGTTC 
ACCATGGGTG GCCTGACCAT CACGACGACG GCGCCCAGCA TCCCCAACCT ATCCAGTATT 
AACGAGAAGA CCAACTCCGG AAATTCCATA AACAGCAGCA ATTACAGCAA TAACAATAGC 
CTGAGCACGC TGACCGGAGG AGGAAGTGCT GCCAGCGGAC CCGGAGGAGG CATCACCATT 
GTGGCCGGTT CCGGGAACCA TGGCGGCGCT GGTGGAGGAG GAGGAGGCGG CGGTAGACAC 
AAGTCGAATC CCCGCCGTCT TAGCATCAAG CAGGATCAGC TGCCCATCGA TTCCATTTGT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Irk1 ( NM_001170226.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163697.1 Drosophila melanogaster inwardly rectifying potassium channel 1, transcript variant C (Irk1), mRNA.

Price & Availability↑

CloneID	OFa07594
Clone ID Related Accession (Same CDS sequence)	NM_001170226.1 , NM_001275934.1
Accession Version	NM_001170226.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	inwardly rectifying potassium channel 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTTGGT ACCGGCAGAC ACGTTTCAGT TCGCGGCGTG TTCGAAAACG TGTGGTCTTC 
AAGCACGGCG AGTGCAACGT TGTGCAGGGA AATGTGGCCA AGAGACGGCG TCGCTATCTG 
CAGGACATCT TCACCACCCT GGTTGACGCC CAGTGGCGCT GGACGCTGCT CGTCTTCGCC 
GCCAGCTTCG TGTTCTCGTG GGCCTTCTTT GGATTCATCT GGTGGATCAT CGCCTACGCA 
CACAATGATC TGGAGTACAC CAATCTCAAG AACCAGTCAC CCGATCTGGT GGCCAACATA 
ACGCACACGG TCTGCGTAAC ACAGGTCTCC AATATGATGT CCGCCTTCCT GTACTCCGTG 
GAAACCCAGA CGACGATAGG CTATGGTAAT CGCTATGTGA CGGAGGAGTG CCCGGAGGCG 
ATATTCACGA TGTGCATCCA GTGCATCACG GGTGTCTTCA TCCAGGCGTT CATGGTGGGC 
ATTGTGTTTG CCAAGTTGTC GCGTCCCAAG AAACGTGCCC AGACGCTGCT CTTCTCGCGC 
AATGCTGTTA TCTGCCACCG GGATGGAGTT CCATGCCTGA TGTTCCGTGT GGGAGACATG 
CGCAAGTCGC ACATCATCGA GGCCCATGTG CGGGCCCAGA TCATCCGCAA GAAGGTGACA 
AAGGAGGGCG AGGTGCTGCC CTTCTACCAG CAGGAGTTGC ACATCGGAGC CGATGGTGGA 
GAGGATCGGC TAATGTTCAT CTGGCCCACT ACCATAGTGC ACAAAATTGA TAGGAACAGT 
CCACTGTACA TGCTCTCCGC CTCCGATATG CTAAAGGAAC GCTTCGAAGT GGTGGTTATG 
CTGGAGGGCG TCATCGAGTC CACTGGCATG ACCACACAGG CCAGAAGCAG CTACTTGCCC 
TCGGAGGTGC TGTGGGGCCA TCGTTTCGTG AATGTGGTGT CCTTCCGCAA GGAGACCGGC 
GAATACGAGG TGGACTACAC GCTATTCAAC AACACCTACG ACGTGGACAC TCCGCTGTGC 
AGCGCCAAGC AGCTGGACGA GCTTAAGTCG GAGTACTCGA AGAGCGCCAA GTGTGTGAAT 
GCACCCTTTG CGGATCGCAC GCTCTCGGCC AGCTTGTTCC AGCGTATTGC CTCCGCCGCC 
TCGGTGGATC ATCTGGATCC GGCTAGCGAC GAATCGCTGG ACTCTGGCCG CCTGCAGATA 
CGCTCCCATT CCATACCCAA CGGCGTGCTG GCCAGCGAGC TGGAGCCGCT GAATAACAAC 
AAGCATGGCG CTTCGTTCAC CATGGGTGGC CTGACCATCA CGACGACGGC GCCCAGCATC 
CCCAACCTAT CCAGTATTAA CGAGAAGACC AACTCCGGAA ATTCCATAAA CAGCAGCAAT 
TACAGCAATA ACAATAGCCT GAGCACGCTG ACCGGAGGAG GAAGTGCTGC CAGCGGACCC 
GGAGGAGGCA TCACCATTGT GGCCGGTTCC GGGAACCATG GCGGCGCTGG TGGAGGAGGA 
GGAGGCGGCG GTAGACACAA GTCGAATCCC CGCCGTCTTA GCATCAAGCA GGATCAGCTG 
CCCATCGATT CCATTTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163697.1
CDS	30..1610
Translation	MIWYRQTRFSSRRVRKRVVFKHGECNVVQGNVAKRRRRYLQDIFTTLVDAQWRWTLLVFAASFVFSWAFFGFIWWIIAYAHNDLEYTNLKNQSPDLVANITHTVCVTQVSNMMSAFLYSVETQTTIGYGNRYVTEECPEAIFTMCIQCITGVFIQAFMVGIVFAKLSRPKKRAQTLLFSRNAVICHRDGVPCLMFRVGDMRKSHIIEAHVRAQIIRKKVTKEGEVLPFYQQELHIGADGGEDRLMFIWPTTIVHKIDRNSPLYMLSASDMLKERFEVVVMLEGVIESTGMTTQARSSYLPSEVLWGHRFVNVVSFRKETGEYEVDYTLFNNTYDVDTPLCSAKQLDELKSEYSKSAKCVNAPFADRTLSASLFQRIASAASVDHLDPASDESLDSGRLQIRSHSIPNGVLASELEPLNNNKHGASFTMGGLTITTTAPSIPNLSSINEKTNSGNSINSSNYSNNNSLSTLTGGGSAASGPGGGITIVAGSGNHGGAGGGGGGGGRHKSNPRRLSIKQDQLPIDSIC

Target ORF information:

RefSeq Version	NM_001170226.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster inwardly rectifying potassium channel 1, transcript variant C (Irk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170226.1

ORF Insert Sequence:

ATGATTTGGT ACCGGCAGAC ACGTTTCAGT TCGCGGCGTG TTCGAAAACG TGTGGTCTTC 
AAGCACGGCG AGTGCAACGT TGTGCAGGGA AATGTGGCCA AGAGACGGCG TCGCTATCTG 
CAGGACATCT TCACCACCCT GGTTGACGCC CAGTGGCGCT GGACGCTGCT CGTCTTCGCC 
GCCAGCTTCG TGTTCTCGTG GGCCTTCTTT GGATTCATCT GGTGGATCAT CGCCTACGCA 
CACAATGATC TGGAGTACAC CAATCTCAAG AACCAGTCAC CCGATCTGGT GGCCAACATA 
ACGCACACGG TCTGCGTAAC ACAGGTCTCC AATATGATGT CCGCCTTCCT GTACTCCGTG 
GAAACCCAGA CGACGATAGG CTATGGTAAT CGCTATGTGA CGGAGGAGTG CCCGGAGGCG 
ATATTCACGA TGTGCATCCA GTGCATCACG GGTGTCTTCA TCCAGGCGTT CATGGTGGGC 
ATTGTGTTTG CCAAGTTGTC GCGTCCCAAG AAACGTGCCC AGACGCTGCT CTTCTCGCGC 
AATGCTGTTA TCTGCCACCG GGATGGAGTT CCATGCCTGA TGTTCCGTGT GGGAGACATG 
CGCAAGTCGC ACATCATCGA GGCCCATGTG CGGGCCCAGA TCATCCGCAA GAAGGTGACA 
AAGGAGGGCG AGGTGCTGCC CTTCTACCAG CAGGAGTTGC ACATCGGAGC CGATGGTGGA 
GAGGATCGGC TAATGTTCAT CTGGCCCACT ACCATAGTGC ACAAAATTGA TAGGAACAGT 
CCACTGTACA TGCTCTCCGC CTCCGATATG CTAAAGGAAC GCTTCGAAGT GGTGGTTATG 
CTGGAGGGCG TCATCGAGTC CACTGGCATG ACCACACAGG CCAGAAGCAG CTACTTGCCC 
TCGGAGGTGC TGTGGGGCCA TCGTTTCGTG AATGTGGTGT CCTTCCGCAA GGAGACCGGC 
GAATACGAGG TGGACTACAC GCTATTCAAC AACACCTACG ACGTGGACAC TCCGCTGTGC 
AGCGCCAAGC AGCTGGACGA GCTTAAGTCG GAGTACTCGA AGAGCGCCAA GTGTGTGAAT 
GCACCCTTTG CGGATCGCAC GCTCTCGGCC AGCTTGTTCC AGCGTATTGC CTCCGCCGCC 
TCGGTGGATC ATCTGGATCC GGCTAGCGAC GAATCGCTGG ACTCTGGCCG CCTGCAGATA 
CGCTCCCATT CCATACCCAA CGGCGTGCTG GCCAGCGAGC TGGAGCCGCT GAATAACAAC 
AAGCATGGCG CTTCGTTCAC CATGGGTGGC CTGACCATCA CGACGACGGC GCCCAGCATC 
CCCAACCTAT CCAGTATTAA CGAGAAGACC AACTCCGGAA ATTCCATAAA CAGCAGCAAT 
TACAGCAATA ACAATAGCCT GAGCACGCTG ACCGGAGGAG GAAGTGCTGC CAGCGGACCC 
GGAGGAGGCA TCACCATTGT GGCCGGTTCC GGGAACCATG GCGGCGCTGG TGGAGGAGGA 
GGAGGCGGCG GTAGACACAA GTCGAATCCC CGCCGTCTTA GCATCAAGCA GGATCAGCTG 
CCCATCGATT CCATTTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Irk1 ( NM_001275934.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262863.1 Drosophila melanogaster inwardly rectifying potassium channel 1, transcript variant H (Irk1), mRNA.

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CloneID	OFa07594
Clone ID Related Accession (Same CDS sequence)	NM_001170226.1 , NM_001275934.1
Accession Version	NM_001275934.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	inwardly rectifying potassium channel 1, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTTGGT ACCGGCAGAC ACGTTTCAGT TCGCGGCGTG TTCGAAAACG TGTGGTCTTC 
AAGCACGGCG AGTGCAACGT TGTGCAGGGA AATGTGGCCA AGAGACGGCG TCGCTATCTG 
CAGGACATCT TCACCACCCT GGTTGACGCC CAGTGGCGCT GGACGCTGCT CGTCTTCGCC 
GCCAGCTTCG TGTTCTCGTG GGCCTTCTTT GGATTCATCT GGTGGATCAT CGCCTACGCA 
CACAATGATC TGGAGTACAC CAATCTCAAG AACCAGTCAC CCGATCTGGT GGCCAACATA 
ACGCACACGG TCTGCGTAAC ACAGGTCTCC AATATGATGT CCGCCTTCCT GTACTCCGTG 
GAAACCCAGA CGACGATAGG CTATGGTAAT CGCTATGTGA CGGAGGAGTG CCCGGAGGCG 
ATATTCACGA TGTGCATCCA GTGCATCACG GGTGTCTTCA TCCAGGCGTT CATGGTGGGC 
ATTGTGTTTG CCAAGTTGTC GCGTCCCAAG AAACGTGCCC AGACGCTGCT CTTCTCGCGC 
AATGCTGTTA TCTGCCACCG GGATGGAGTT CCATGCCTGA TGTTCCGTGT GGGAGACATG 
CGCAAGTCGC ACATCATCGA GGCCCATGTG CGGGCCCAGA TCATCCGCAA GAAGGTGACA 
AAGGAGGGCG AGGTGCTGCC CTTCTACCAG CAGGAGTTGC ACATCGGAGC CGATGGTGGA 
GAGGATCGGC TAATGTTCAT CTGGCCCACT ACCATAGTGC ACAAAATTGA TAGGAACAGT 
CCACTGTACA TGCTCTCCGC CTCCGATATG CTAAAGGAAC GCTTCGAAGT GGTGGTTATG 
CTGGAGGGCG TCATCGAGTC CACTGGCATG ACCACACAGG CCAGAAGCAG CTACTTGCCC 
TCGGAGGTGC TGTGGGGCCA TCGTTTCGTG AATGTGGTGT CCTTCCGCAA GGAGACCGGC 
GAATACGAGG TGGACTACAC GCTATTCAAC AACACCTACG ACGTGGACAC TCCGCTGTGC 
AGCGCCAAGC AGCTGGACGA GCTTAAGTCG GAGTACTCGA AGAGCGCCAA GTGTGTGAAT 
GCACCCTTTG CGGATCGCAC GCTCTCGGCC AGCTTGTTCC AGCGTATTGC CTCCGCCGCC 
TCGGTGGATC ATCTGGATCC GGCTAGCGAC GAATCGCTGG ACTCTGGCCG CCTGCAGATA 
CGCTCCCATT CCATACCCAA CGGCGTGCTG GCCAGCGAGC TGGAGCCGCT GAATAACAAC 
AAGCATGGCG CTTCGTTCAC CATGGGTGGC CTGACCATCA CGACGACGGC GCCCAGCATC 
CCCAACCTAT CCAGTATTAA CGAGAAGACC AACTCCGGAA ATTCCATAAA CAGCAGCAAT 
TACAGCAATA ACAATAGCCT GAGCACGCTG ACCGGAGGAG GAAGTGCTGC CAGCGGACCC 
GGAGGAGGCA TCACCATTGT GGCCGGTTCC GGGAACCATG GCGGCGCTGG TGGAGGAGGA 
GGAGGCGGCG GTAGACACAA GTCGAATCCC CGCCGTCTTA GCATCAAGCA GGATCAGCTG 
CCCATCGATT CCATTTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262863.1
CDS	453..2033
Translation	MIWYRQTRFSSRRVRKRVVFKHGECNVVQGNVAKRRRRYLQDIFTTLVDAQWRWTLLVFAASFVFSWAFFGFIWWIIAYAHNDLEYTNLKNQSPDLVANITHTVCVTQVSNMMSAFLYSVETQTTIGYGNRYVTEECPEAIFTMCIQCITGVFIQAFMVGIVFAKLSRPKKRAQTLLFSRNAVICHRDGVPCLMFRVGDMRKSHIIEAHVRAQIIRKKVTKEGEVLPFYQQELHIGADGGEDRLMFIWPTTIVHKIDRNSPLYMLSASDMLKERFEVVVMLEGVIESTGMTTQARSSYLPSEVLWGHRFVNVVSFRKETGEYEVDYTLFNNTYDVDTPLCSAKQLDELKSEYSKSAKCVNAPFADRTLSASLFQRIASAASVDHLDPASDESLDSGRLQIRSHSIPNGVLASELEPLNNNKHGASFTMGGLTITTTAPSIPNLSSINEKTNSGNSINSSNYSNNNSLSTLTGGGSAASGPGGGITIVAGSGNHGGAGGGGGGGGRHKSNPRRLSIKQDQLPIDSIC

Target ORF information:

RefSeq Version	NM_001275934.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster inwardly rectifying potassium channel 1, transcript variant H (Irk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275934.1

ORF Insert Sequence:

ATGATTTGGT ACCGGCAGAC ACGTTTCAGT TCGCGGCGTG TTCGAAAACG TGTGGTCTTC 
AAGCACGGCG AGTGCAACGT TGTGCAGGGA AATGTGGCCA AGAGACGGCG TCGCTATCTG 
CAGGACATCT TCACCACCCT GGTTGACGCC CAGTGGCGCT GGACGCTGCT CGTCTTCGCC 
GCCAGCTTCG TGTTCTCGTG GGCCTTCTTT GGATTCATCT GGTGGATCAT CGCCTACGCA 
CACAATGATC TGGAGTACAC CAATCTCAAG AACCAGTCAC CCGATCTGGT GGCCAACATA 
ACGCACACGG TCTGCGTAAC ACAGGTCTCC AATATGATGT CCGCCTTCCT GTACTCCGTG 
GAAACCCAGA CGACGATAGG CTATGGTAAT CGCTATGTGA CGGAGGAGTG CCCGGAGGCG 
ATATTCACGA TGTGCATCCA GTGCATCACG GGTGTCTTCA TCCAGGCGTT CATGGTGGGC 
ATTGTGTTTG CCAAGTTGTC GCGTCCCAAG AAACGTGCCC AGACGCTGCT CTTCTCGCGC 
AATGCTGTTA TCTGCCACCG GGATGGAGTT CCATGCCTGA TGTTCCGTGT GGGAGACATG 
CGCAAGTCGC ACATCATCGA GGCCCATGTG CGGGCCCAGA TCATCCGCAA GAAGGTGACA 
AAGGAGGGCG AGGTGCTGCC CTTCTACCAG CAGGAGTTGC ACATCGGAGC CGATGGTGGA 
GAGGATCGGC TAATGTTCAT CTGGCCCACT ACCATAGTGC ACAAAATTGA TAGGAACAGT 
CCACTGTACA TGCTCTCCGC CTCCGATATG CTAAAGGAAC GCTTCGAAGT GGTGGTTATG 
CTGGAGGGCG TCATCGAGTC CACTGGCATG ACCACACAGG CCAGAAGCAG CTACTTGCCC 
TCGGAGGTGC TGTGGGGCCA TCGTTTCGTG AATGTGGTGT CCTTCCGCAA GGAGACCGGC 
GAATACGAGG TGGACTACAC GCTATTCAAC AACACCTACG ACGTGGACAC TCCGCTGTGC 
AGCGCCAAGC AGCTGGACGA GCTTAAGTCG GAGTACTCGA AGAGCGCCAA GTGTGTGAAT 
GCACCCTTTG CGGATCGCAC GCTCTCGGCC AGCTTGTTCC AGCGTATTGC CTCCGCCGCC 
TCGGTGGATC ATCTGGATCC GGCTAGCGAC GAATCGCTGG ACTCTGGCCG CCTGCAGATA 
CGCTCCCATT CCATACCCAA CGGCGTGCTG GCCAGCGAGC TGGAGCCGCT GAATAACAAC 
AAGCATGGCG CTTCGTTCAC CATGGGTGGC CTGACCATCA CGACGACGGC GCCCAGCATC 
CCCAACCTAT CCAGTATTAA CGAGAAGACC AACTCCGGAA ATTCCATAAA CAGCAGCAAT 
TACAGCAATA ACAATAGCCT GAGCACGCTG ACCGGAGGAG GAAGTGCTGC CAGCGGACCC 
GGAGGAGGCA TCACCATTGT GGCCGGTTCC GGGAACCATG GCGGCGCTGG TGGAGGAGGA 
GGAGGCGGCG GTAGACACAA GTCGAATCCC CGCCGTCTTA GCATCAAGCA GGATCAGCTG 
CCCATCGATT CCATTTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Irk1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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