Cow cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cow
Entrez Gene ID	42733
Full Name	Carrier of Wingless
Synonyms	CG13830,Dmel\CG13830,cow
General protein information	Preferred Names Carrier of Wingless Names CG13830 gene product from transcript CG13830-RD CG13830-PC CG13830-PD CG13830-PE Cow-PC Cow-PD Cow-PE carrier of wingless
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 94D12-94D13

mRNA and Protein(s)

mRNA	Protein	Name
NM_001104413.3	NP_001097883.2	carrier of wingless, isoform D
NM_001170224.2	NP_001163695.1	carrier of wingless, isoform C
NM_001275928.1	NP_001262857.1	carrier of wingless, isoform E

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003400	XP_311685.5
Drosophila melanogaster (fruit fly)	CG13830	NP_001262857.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cow promotes the apical transport of Wg, independent of Dlp and endocytosis.
Title: Carrier of Wingless (Cow), a secreted heparan sulfate proteoglycan, promotes extracellular transport of Wingless.

The following Cow gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cow cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05749	NM_001170224.2 Latest version!	Drosophila melanogaster carrier of wingless, transcript variant C (Cow), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa05749	NM_001104413.3 Latest version!	Drosophila melanogaster carrier of wingless, transcript variant D (Cow), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa07587	NM_001275928.1 Latest version!	Drosophila melanogaster carrier of wingless, transcript variant E (Cow), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cow ( NM_001104413.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097883.2 Drosophila melanogaster carrier of wingless, transcript variant D (Cow), mRNA.

Price & Availability↑

CloneID	OFa05749
Clone ID Related Accession (Same CDS sequence)	NM_001104413.3 , NM_001170224.2
Accession Version	NM_001104413.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	carrier of wingless, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001104413.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCACT CGCCGCTGAT TGCCAGCGCT TGTCTGGCGC TGGTGTTGAT GTCATCCAGC 
CTGATTGGCA GCACCGAGGC CAGAAACAAG AAGAAGTACG TCGGCGAGAC GGGCGGCGAT 
TTCGAGTTTA TCGATGAGAT AAACAAAAAC ACGCAGAGCA ACAAGAATCT GGGCGAGCAC 
AAGCGCTGGA TACACGATCC CTCGAGTGAC CTTTGCCGAC CGCTGAACTG CAAGAAGCGT 
GAAATCTGCC TGCTGGAGGA CGAGTTCAGT GCCGTTTGCG TGTCGAAGAA GGAGCTCCAC 
AAGAATCGTG ATGAGATAAT CACCAAGGCC AAGTATTTGG AGGAGGAGGC CAAGCGGCGT 
GTCAACCAGC AGGACAATCA GGACAGCCAG GACGCGGAGG ATATCAACAA CGACGACGAG 
GATAACAGCA GCGATGGCGG CAGCAGCAAC TCCAGCCCCA CAGGCACCAA CAATGCCCAG 
GCCAGTGTCC AGGGAAATGA GGAGACCGAC GACGAAGATA AGAGCCTAAG TCTGGGCGAC 
GACGACGAGT CCAAGGAGGA TGATGTCTTC TACGAGAACA GTATCGCCGC CGGCGATAAG 
CAGCAGCAGC AACAGCAGCT ATCCCAGCCT GCTGCAGGAC CTTCCGTTAT TCAGCAGGAC 
GACGACGAGG AGTTGGACAA CTGCAAGCCG TGTCCTGTGG CCAAGCCGAC GTTCCTGTGC 
GGAGCCGACA ACAGGACCTA CTCATCACTA TGCAGATTGG ATTATCACAA TTGCATACAT 
TCGACGTCCA TACGGATCGC CTGCAAAGGA TTCTGTCCCT GCAAGGAGAT TGTCGATGGA 
AAGCGGCTGC AGCGCATCTC GGGCTACAAC AACAAGTACA ACAAGAAGAT CAGCCTGGAC 
CAGCAGCAGC AGCAGCAGCA ACAACAACAA CAGCAGCAGC AGCAGCAGCA GGCCTACAAG 
GACAGCAACA ACAACAACAT CATGATGAAC AGCGGCAACA TAATGGGCGG CAACAACAAC 
GACTTCAACA CGATCATGAA TGACAAAGAG GACAATAACC GGCACAACAA CCACATCAAT 
GCCCAGTACA CTTTCACGCC CGAGGAGATC AAATATGACA ACAAGCACTA CAAGTACCTC 
AAGTACACGG CCTACAAGAA GGACTCCAAG TACCAGGAGG ACAAGCACAA GATGCGCAAC 
TATAACGAGG TGGTCGAGAA GCAGCAGCAG AAGTTCAATA AGAACAGCAA CTTGAATGCC 
TATCCATCGA AATCGGCAGA GTGCAAGCCG CAGCAACTGA CGGCGATTGG CAACCGACTG 
CTGGACTGGT TCTCGGTGAT CATGGCGGAC AGCAAGAAGC GGCGCCAGCA CAGCCAGAAG 
TCAAAGGCCC ACTTCCCGCC CGCCTGCAAG ACGGAGGCCA AGTGGATGTT CGGCCACCTG 
GATCTGAATA ACGACGGGCA GCTGTCGCTG CAGGAGATGT ACGACCTGGA GCATGACCAG 
AACGAGCGTT GCATCAAGCC ATTCATCGAC ACCTGCGACT TGGACACGGA TAGCTCGATC 
AACACACGGG AATGGTGCAG ATGCTTCGAG AAGACGGATC GTCCATGTGC CGCTGTGCGC 
CGGAGAATCG CCGGCGACTT TGCAGGAGCC TACGCACCGG ACTGCGACAT CCAGGGATTC 
TACAAGCCTA CCCAGTGCCA CAATTCCGTG GGAGTTTGCT GGTGCGTGGA CAAGCACGGA 
GTGGAGTTCG CCAACACACG CACACGCGGC AAACCCAATT GCGAGTCCGT GGTTAATAAT 
GCCGCATCGC TCACATCCGA TGACGAGGAC GAGGGCGCCG ATGACGAGGA CAGCGCGGAG 
GGCAGTGCCG ATCAGATGCT GGTCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097883.2
CDS	387..2276
Translation	MKHSPLIASACLALVLMSSSLIGSTEARNKKKYVGETGGDFEFIDEINKNTQSNKNLGEHKRWIHDPSSDLCRPLNCKKREICLLEDEFSAVCVSKKELHKNRDEIITKAKYLEEEAKRRVNQQDNQDSQDAEDINNDDEDNSSDGGSSNSSPTGTNNAQASVQGNEETDDEDKSLSLGDDDESKEDDVFYENSIAAGDKQQQQQQLSQPAAGPSVIQQDDDEELDNCKPCPVAKPTFLCGADNRTYSSLCRLDYHNCIHSTSIRIACKGFCPCKEIVDGKRLQRISGYNNKYNKKISLDQQQQQQQQQQQQQQQQQAYKDSNNNNIMMNSGNIMGGNNNDFNTIMNDKEDNNRHNNHINAQYTFTPEEIKYDNKHYKYLKYTAYKKDSKYQEDKHKMRNYNEVVEKQQQKFNKNSNLNAYPSKSAECKPQQLTAIGNRLLDWFSVIMADSKKRRQHSQKSKAHFPPACKTEAKWMFGHLDLNNDGQLSLQEMYDLEHDQNERCIKPFIDTCDLDTDSSINTREWCRCFEKTDRPCAAVRRRIAGDFAGAYAPDCDIQGFYKPTQCHNSVGVCWCVDKHGVEFANTRTRGKPNCESVVNNAASLTSDDEDEGADDEDSAEGSADQMLVF

Target ORF information:

RefSeq Version	NM_001104413.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster carrier of wingless, transcript variant D (Cow), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104413.3

ORF Insert Sequence:

ATGAAGCACT CGCCGCTGAT TGCCAGCGCT TGTCTGGCGC TGGTGTTGAT GTCATCCAGC 
CTGATTGGCA GCACCGAGGC CAGAAACAAG AAGAAGTACG TCGGCGAGAC GGGCGGCGAT 
TTCGAGTTTA TCGATGAGAT AAACAAAAAC ACGCAGAGCA ACAAGAATCT GGGCGAGCAC 
AAGCGCTGGA TACACGATCC CTCGAGTGAC CTTTGCCGAC CGCTGAACTG CAAGAAGCGT 
GAAATCTGCC TGCTGGAGGA CGAGTTCAGT GCCGTTTGCG TGTCGAAGAA GGAGCTCCAC 
AAGAATCGTG ATGAGATAAT CACCAAGGCC AAGTATTTGG AGGAGGAGGC CAAGCGGCGT 
GTCAACCAGC AGGACAATCA GGACAGCCAG GACGCGGAGG ATATCAACAA CGACGACGAG 
GATAACAGCA GCGATGGCGG CAGCAGCAAC TCCAGCCCCA CAGGCACCAA CAATGCCCAG 
GCCAGTGTCC AGGGAAATGA GGAGACCGAC GACGAAGATA AGAGCCTAAG TCTGGGCGAC 
GACGACGAGT CCAAGGAGGA TGATGTCTTC TACGAGAACA GTATCGCCGC CGGCGATAAG 
CAGCAGCAGC AACAGCAGCT ATCCCAGCCT GCTGCAGGAC CTTCCGTTAT TCAGCAGGAC 
GACGACGAGG AGTTGGACAA CTGCAAGCCG TGTCCTGTGG CCAAGCCGAC GTTCCTGTGC 
GGAGCCGACA ACAGGACCTA CTCATCACTA TGCAGATTGG ATTATCACAA TTGCATACAT 
TCGACGTCCA TACGGATCGC CTGCAAAGGA TTCTGTCCCT GCAAGGAGAT TGTCGATGGA 
AAGCGGCTGC AGCGCATCTC GGGCTACAAC AACAAGTACA ACAAGAAGAT CAGCCTGGAC 
CAGCAGCAGC AGCAGCAGCA ACAACAACAA CAGCAGCAGC AGCAGCAGCA GGCCTACAAG 
GACAGCAACA ACAACAACAT CATGATGAAC AGCGGCAACA TAATGGGCGG CAACAACAAC 
GACTTCAACA CGATCATGAA TGACAAAGAG GACAATAACC GGCACAACAA CCACATCAAT 
GCCCAGTACA CTTTCACGCC CGAGGAGATC AAATATGACA ACAAGCACTA CAAGTACCTC 
AAGTACACGG CCTACAAGAA GGACTCCAAG TACCAGGAGG ACAAGCACAA GATGCGCAAC 
TATAACGAGG TGGTCGAGAA GCAGCAGCAG AAGTTCAATA AGAACAGCAA CTTGAATGCC 
TATCCATCGA AATCGGCAGA GTGCAAGCCG CAGCAACTGA CGGCGATTGG CAACCGACTG 
CTGGACTGGT TCTCGGTGAT CATGGCGGAC AGCAAGAAGC GGCGCCAGCA CAGCCAGAAG 
TCAAAGGCCC ACTTCCCGCC CGCCTGCAAG ACGGAGGCCA AGTGGATGTT CGGCCACCTG 
GATCTGAATA ACGACGGGCA GCTGTCGCTG CAGGAGATGT ACGACCTGGA GCATGACCAG 
AACGAGCGTT GCATCAAGCC ATTCATCGAC ACCTGCGACT TGGACACGGA TAGCTCGATC 
AACACACGGG AATGGTGCAG ATGCTTCGAG AAGACGGATC GTCCATGTGC CGCTGTGCGC 
CGGAGAATCG CCGGCGACTT TGCAGGAGCC TACGCACCGG ACTGCGACAT CCAGGGATTC 
TACAAGCCTA CCCAGTGCCA CAATTCCGTG GGAGTTTGCT GGTGCGTGGA CAAGCACGGA 
GTGGAGTTCG CCAACACACG CACACGCGGC AAACCCAATT GCGAGTCCGT GGTTAATAAT 
GCCGCATCGC TCACATCCGA TGACGAGGAC GAGGGCGCCG ATGACGAGGA CAGCGCGGAG 
GGCAGTGCCG ATCAGATGCT GGTCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cow ( NM_001170224.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163695.1 Drosophila melanogaster carrier of wingless, transcript variant C (Cow), mRNA.

Price & Availability↑

CloneID	OFa05749
Clone ID Related Accession (Same CDS sequence)	NM_001104413.3 , NM_001170224.2
Accession Version	NM_001170224.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	carrier of wingless, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170224.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCACT CGCCGCTGAT TGCCAGCGCT TGTCTGGCGC TGGTGTTGAT GTCATCCAGC 
CTGATTGGCA GCACCGAGGC CAGAAACAAG AAGAAGTACG TCGGCGAGAC GGGCGGCGAT 
TTCGAGTTTA TCGATGAGAT AAACAAAAAC ACGCAGAGCA ACAAGAATCT GGGCGAGCAC 
AAGCGCTGGA TACACGATCC CTCGAGTGAC CTTTGCCGAC CGCTGAACTG CAAGAAGCGT 
GAAATCTGCC TGCTGGAGGA CGAGTTCAGT GCCGTTTGCG TGTCGAAGAA GGAGCTCCAC 
AAGAATCGTG ATGAGATAAT CACCAAGGCC AAGTATTTGG AGGAGGAGGC CAAGCGGCGT 
GTCAACCAGC AGGACAATCA GGACAGCCAG GACGCGGAGG ATATCAACAA CGACGACGAG 
GATAACAGCA GCGATGGCGG CAGCAGCAAC TCCAGCCCCA CAGGCACCAA CAATGCCCAG 
GCCAGTGTCC AGGGAAATGA GGAGACCGAC GACGAAGATA AGAGCCTAAG TCTGGGCGAC 
GACGACGAGT CCAAGGAGGA TGATGTCTTC TACGAGAACA GTATCGCCGC CGGCGATAAG 
CAGCAGCAGC AACAGCAGCT ATCCCAGCCT GCTGCAGGAC CTTCCGTTAT TCAGCAGGAC 
GACGACGAGG AGTTGGACAA CTGCAAGCCG TGTCCTGTGG CCAAGCCGAC GTTCCTGTGC 
GGAGCCGACA ACAGGACCTA CTCATCACTA TGCAGATTGG ATTATCACAA TTGCATACAT 
TCGACGTCCA TACGGATCGC CTGCAAAGGA TTCTGTCCCT GCAAGGAGAT TGTCGATGGA 
AAGCGGCTGC AGCGCATCTC GGGCTACAAC AACAAGTACA ACAAGAAGAT CAGCCTGGAC 
CAGCAGCAGC AGCAGCAGCA ACAACAACAA CAGCAGCAGC AGCAGCAGCA GGCCTACAAG 
GACAGCAACA ACAACAACAT CATGATGAAC AGCGGCAACA TAATGGGCGG CAACAACAAC 
GACTTCAACA CGATCATGAA TGACAAAGAG GACAATAACC GGCACAACAA CCACATCAAT 
GCCCAGTACA CTTTCACGCC CGAGGAGATC AAATATGACA ACAAGCACTA CAAGTACCTC 
AAGTACACGG CCTACAAGAA GGACTCCAAG TACCAGGAGG ACAAGCACAA GATGCGCAAC 
TATAACGAGG TGGTCGAGAA GCAGCAGCAG AAGTTCAATA AGAACAGCAA CTTGAATGCC 
TATCCATCGA AATCGGCAGA GTGCAAGCCG CAGCAACTGA CGGCGATTGG CAACCGACTG 
CTGGACTGGT TCTCGGTGAT CATGGCGGAC AGCAAGAAGC GGCGCCAGCA CAGCCAGAAG 
TCAAAGGCCC ACTTCCCGCC CGCCTGCAAG ACGGAGGCCA AGTGGATGTT CGGCCACCTG 
GATCTGAATA ACGACGGGCA GCTGTCGCTG CAGGAGATGT ACGACCTGGA GCATGACCAG 
AACGAGCGTT GCATCAAGCC ATTCATCGAC ACCTGCGACT TGGACACGGA TAGCTCGATC 
AACACACGGG AATGGTGCAG ATGCTTCGAG AAGACGGATC GTCCATGTGC CGCTGTGCGC 
CGGAGAATCG CCGGCGACTT TGCAGGAGCC TACGCACCGG ACTGCGACAT CCAGGGATTC 
TACAAGCCTA CCCAGTGCCA CAATTCCGTG GGAGTTTGCT GGTGCGTGGA CAAGCACGGA 
GTGGAGTTCG CCAACACACG CACACGCGGC AAACCCAATT GCGAGTCCGT GGTTAATAAT 
GCCGCATCGC TCACATCCGA TGACGAGGAC GAGGGCGCCG ATGACGAGGA CAGCGCGGAG 
GGCAGTGCCG ATCAGATGCT GGTCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163695.1
CDS	387..2276
Translation	MKHSPLIASACLALVLMSSSLIGSTEARNKKKYVGETGGDFEFIDEINKNTQSNKNLGEHKRWIHDPSSDLCRPLNCKKREICLLEDEFSAVCVSKKELHKNRDEIITKAKYLEEEAKRRVNQQDNQDSQDAEDINNDDEDNSSDGGSSNSSPTGTNNAQASVQGNEETDDEDKSLSLGDDDESKEDDVFYENSIAAGDKQQQQQQLSQPAAGPSVIQQDDDEELDNCKPCPVAKPTFLCGADNRTYSSLCRLDYHNCIHSTSIRIACKGFCPCKEIVDGKRLQRISGYNNKYNKKISLDQQQQQQQQQQQQQQQQQAYKDSNNNNIMMNSGNIMGGNNNDFNTIMNDKEDNNRHNNHINAQYTFTPEEIKYDNKHYKYLKYTAYKKDSKYQEDKHKMRNYNEVVEKQQQKFNKNSNLNAYPSKSAECKPQQLTAIGNRLLDWFSVIMADSKKRRQHSQKSKAHFPPACKTEAKWMFGHLDLNNDGQLSLQEMYDLEHDQNERCIKPFIDTCDLDTDSSINTREWCRCFEKTDRPCAAVRRRIAGDFAGAYAPDCDIQGFYKPTQCHNSVGVCWCVDKHGVEFANTRTRGKPNCESVVNNAASLTSDDEDEGADDEDSAEGSADQMLVF

Target ORF information:

RefSeq Version	NM_001170224.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster carrier of wingless, transcript variant C (Cow), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170224.2

ORF Insert Sequence:

ATGAAGCACT CGCCGCTGAT TGCCAGCGCT TGTCTGGCGC TGGTGTTGAT GTCATCCAGC 
CTGATTGGCA GCACCGAGGC CAGAAACAAG AAGAAGTACG TCGGCGAGAC GGGCGGCGAT 
TTCGAGTTTA TCGATGAGAT AAACAAAAAC ACGCAGAGCA ACAAGAATCT GGGCGAGCAC 
AAGCGCTGGA TACACGATCC CTCGAGTGAC CTTTGCCGAC CGCTGAACTG CAAGAAGCGT 
GAAATCTGCC TGCTGGAGGA CGAGTTCAGT GCCGTTTGCG TGTCGAAGAA GGAGCTCCAC 
AAGAATCGTG ATGAGATAAT CACCAAGGCC AAGTATTTGG AGGAGGAGGC CAAGCGGCGT 
GTCAACCAGC AGGACAATCA GGACAGCCAG GACGCGGAGG ATATCAACAA CGACGACGAG 
GATAACAGCA GCGATGGCGG CAGCAGCAAC TCCAGCCCCA CAGGCACCAA CAATGCCCAG 
GCCAGTGTCC AGGGAAATGA GGAGACCGAC GACGAAGATA AGAGCCTAAG TCTGGGCGAC 
GACGACGAGT CCAAGGAGGA TGATGTCTTC TACGAGAACA GTATCGCCGC CGGCGATAAG 
CAGCAGCAGC AACAGCAGCT ATCCCAGCCT GCTGCAGGAC CTTCCGTTAT TCAGCAGGAC 
GACGACGAGG AGTTGGACAA CTGCAAGCCG TGTCCTGTGG CCAAGCCGAC GTTCCTGTGC 
GGAGCCGACA ACAGGACCTA CTCATCACTA TGCAGATTGG ATTATCACAA TTGCATACAT 
TCGACGTCCA TACGGATCGC CTGCAAAGGA TTCTGTCCCT GCAAGGAGAT TGTCGATGGA 
AAGCGGCTGC AGCGCATCTC GGGCTACAAC AACAAGTACA ACAAGAAGAT CAGCCTGGAC 
CAGCAGCAGC AGCAGCAGCA ACAACAACAA CAGCAGCAGC AGCAGCAGCA GGCCTACAAG 
GACAGCAACA ACAACAACAT CATGATGAAC AGCGGCAACA TAATGGGCGG CAACAACAAC 
GACTTCAACA CGATCATGAA TGACAAAGAG GACAATAACC GGCACAACAA CCACATCAAT 
GCCCAGTACA CTTTCACGCC CGAGGAGATC AAATATGACA ACAAGCACTA CAAGTACCTC 
AAGTACACGG CCTACAAGAA GGACTCCAAG TACCAGGAGG ACAAGCACAA GATGCGCAAC 
TATAACGAGG TGGTCGAGAA GCAGCAGCAG AAGTTCAATA AGAACAGCAA CTTGAATGCC 
TATCCATCGA AATCGGCAGA GTGCAAGCCG CAGCAACTGA CGGCGATTGG CAACCGACTG 
CTGGACTGGT TCTCGGTGAT CATGGCGGAC AGCAAGAAGC GGCGCCAGCA CAGCCAGAAG 
TCAAAGGCCC ACTTCCCGCC CGCCTGCAAG ACGGAGGCCA AGTGGATGTT CGGCCACCTG 
GATCTGAATA ACGACGGGCA GCTGTCGCTG CAGGAGATGT ACGACCTGGA GCATGACCAG 
AACGAGCGTT GCATCAAGCC ATTCATCGAC ACCTGCGACT TGGACACGGA TAGCTCGATC 
AACACACGGG AATGGTGCAG ATGCTTCGAG AAGACGGATC GTCCATGTGC CGCTGTGCGC 
CGGAGAATCG CCGGCGACTT TGCAGGAGCC TACGCACCGG ACTGCGACAT CCAGGGATTC 
TACAAGCCTA CCCAGTGCCA CAATTCCGTG GGAGTTTGCT GGTGCGTGGA CAAGCACGGA 
GTGGAGTTCG CCAACACACG CACACGCGGC AAACCCAATT GCGAGTCCGT GGTTAATAAT 
GCCGCATCGC TCACATCCGA TGACGAGGAC GAGGGCGCCG ATGACGAGGA CAGCGCGGAG 
GGCAGTGCCG ATCAGATGCT GGTCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cow ( NM_001275928.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262857.1 Drosophila melanogaster carrier of wingless, transcript variant E (Cow), mRNA.

Price & Availability↑

CloneID	OFa07587
Clone ID Related Accession (Same CDS sequence)	NM_001275928.1
Accession Version	NM_001275928.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	carrier of wingless, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCACT CGCCGCTGAT TGCCAGCGCT TGTCTGGCGC TGGTGTTGAT GTCATCCAGC 
CTGATTGGCA GCACCGAGGC CAGAAACAAG AAGAAGTACG TCGGCGAGAC GGGCGGCGAT 
TTCGAGTTTA TCGATGAGAT AAACAAAAAC ACGCAGAGCA ACAAGAATCT GGGCGAGCAC 
AAGCGCTGGA TACACGATCC CTCGAGTGAC CTTTGCCGAC CGCTGAACTG CAAGAAGCGT 
GAAATCTGCC TGCTGGAGGA CGAGTTCAGT GCCGTTTGCG TGTCGAAGAA GGAGCTCCAC 
AAGAATCGTG ATGAGATAAT CACCAAGGCC AAGTATTTGG AGGAGGAGGC CAAGCGGCGT 
GTCAACCAGC AGGACAATCA GGACAGCCAG GACGCGGAGG ATATCAACAA CGACGACGAG 
GATAACAGCA GCGATGGCGG CAGCAGCAAC TCCAGCCCCA CAGGCACCAA CAATGCCCAG 
GCCAGTGTCC AGGGAAATGA GGAGACCGAC GACGAAGATA AGAGCCTAAG TCTGGGCGAC 
GACGACGAGT CCAAGGAGGA TGATGTCTTC TACGAGAACA GTATCGCCGC CGGCGATAAG 
CAGCAGCAGC AACAGCAGCT ATCCCAGCCT GCTGCAGGAC CTTCCGTTAT TCAGCAGGAC 
GACGACGAGG AGTTGGACAA CTGCAAGCCG TGTCCTGTGG CCAAGCCGAC GTTCCTGTGC 
GGAGCCGACA ACAGGACCTA CTCATCACTA TGCAGATTGG ATTATCACAA TTGCATACAT 
TCGACGTCCA TACGGATCGC CTGCAAAGGA TTCTGTCCCT GCAAGGAGAT TGTCGATGGA 
AAGCGGCTGC AGCGCATCTC GGGCTACAAC AACAAGTACA ACAAGAAGAT CAGCCTGGAC 
CAGCAGCAGC AGCAGCAGCA ACAACAACAA CAGCAGCAGC AGCAGCAGCA GGCCTACAAG 
GACAGCAACA ACAACAACAT CATGATGAAC AGCGGCAACA TAATGGGCGG CAACAACAAC 
GACTTCAACA CGATCATGAA TGACAAAGAG GACAATAACC GGCACAACAA CCACATCAAT 
GCCCAGTACA CTTTCACGCC CGAGGAGATC AAATATGACA ACAAGCACTA CAAGTACCTC 
AAGTACACGG CCTACAAGAA GGACTCCAAG TACCAGGAGG ACAAGCACAA GATGCGCAAC 
TATAACGAGG TGGTCGAGAA GCAGCAGCAG AAGTTCAATA AGAACAGCAA CTTGAATGCC 
TATCCATCGA AATCGGCAGA GTGCAAGCCG CAGCAACTGA CGGCGATTGG CAACCGACTG 
CTGGACTGGT TCTCGGTGAT CATGGCGGAC AGCAAGAAGC GGCGCCAGCA CAGCCAGAAG 
TCAAAGGCCC ACTTCCCGCC CGCCTGCAAG ACGGAGGCCA AGTGGATGTT CGGCCACCTG 
GATCTGAATA ACGACGGGCA GCTGTCGCTG CAGGAGATGT ACGACCTGGA GCATGACCAG 
AACGAGCGTT GCATCAAGCC ATTCATCGAC ACCTGCGACT TGGACACGGA TAGCTCGATC 
AACACACGGG AATGGTGCAG ATGCTTCGAG AAGACGGATC GTCCATGTGC CGCTGTGCGC 
CGGAGAATCG CCGGCGACTT TGCAGGTGAG ATAGGAGCCT ACGCACCGGA CTGCGACATC 
CAGGGATTCT ACAAGCCTAC CCAGTGCCAC AATTCCGTGG GAGTTTGCTG GTGCGTGGAC 
AAGCACGGAG TGGAGTTCGC CAACACACGC ACACGCGGCA AACCCAATTG CGAGTCCGTG 
GTTAATAATG CCGCATCGCT CACATCCGAT GACGAGGACG AGGGCGCCGA TGACGAGGAC 
AGCGCGGAGG GCAGTGCCGA TCAGATGCTG GTCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262857.1
CDS	387..2285
Translation	MKHSPLIASACLALVLMSSSLIGSTEARNKKKYVGETGGDFEFIDEINKNTQSNKNLGEHKRWIHDPSSDLCRPLNCKKREICLLEDEFSAVCVSKKELHKNRDEIITKAKYLEEEAKRRVNQQDNQDSQDAEDINNDDEDNSSDGGSSNSSPTGTNNAQASVQGNEETDDEDKSLSLGDDDESKEDDVFYENSIAAGDKQQQQQQLSQPAAGPSVIQQDDDEELDNCKPCPVAKPTFLCGADNRTYSSLCRLDYHNCIHSTSIRIACKGFCPCKEIVDGKRLQRISGYNNKYNKKISLDQQQQQQQQQQQQQQQQQAYKDSNNNNIMMNSGNIMGGNNNDFNTIMNDKEDNNRHNNHINAQYTFTPEEIKYDNKHYKYLKYTAYKKDSKYQEDKHKMRNYNEVVEKQQQKFNKNSNLNAYPSKSAECKPQQLTAIGNRLLDWFSVIMADSKKRRQHSQKSKAHFPPACKTEAKWMFGHLDLNNDGQLSLQEMYDLEHDQNERCIKPFIDTCDLDTDSSINTREWCRCFEKTDRPCAAVRRRIAGDFAGEIGAYAPDCDIQGFYKPTQCHNSVGVCWCVDKHGVEFANTRTRGKPNCESVVNNAASLTSDDEDEGADDEDSAEGSADQMLVF

Target ORF information:

RefSeq Version	NM_001275928.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster carrier of wingless, transcript variant E (Cow), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275928.1

ORF Insert Sequence:

ATGAAGCACT CGCCGCTGAT TGCCAGCGCT TGTCTGGCGC TGGTGTTGAT GTCATCCAGC 
CTGATTGGCA GCACCGAGGC CAGAAACAAG AAGAAGTACG TCGGCGAGAC GGGCGGCGAT 
TTCGAGTTTA TCGATGAGAT AAACAAAAAC ACGCAGAGCA ACAAGAATCT GGGCGAGCAC 
AAGCGCTGGA TACACGATCC CTCGAGTGAC CTTTGCCGAC CGCTGAACTG CAAGAAGCGT 
GAAATCTGCC TGCTGGAGGA CGAGTTCAGT GCCGTTTGCG TGTCGAAGAA GGAGCTCCAC 
AAGAATCGTG ATGAGATAAT CACCAAGGCC AAGTATTTGG AGGAGGAGGC CAAGCGGCGT 
GTCAACCAGC AGGACAATCA GGACAGCCAG GACGCGGAGG ATATCAACAA CGACGACGAG 
GATAACAGCA GCGATGGCGG CAGCAGCAAC TCCAGCCCCA CAGGCACCAA CAATGCCCAG 
GCCAGTGTCC AGGGAAATGA GGAGACCGAC GACGAAGATA AGAGCCTAAG TCTGGGCGAC 
GACGACGAGT CCAAGGAGGA TGATGTCTTC TACGAGAACA GTATCGCCGC CGGCGATAAG 
CAGCAGCAGC AACAGCAGCT ATCCCAGCCT GCTGCAGGAC CTTCCGTTAT TCAGCAGGAC 
GACGACGAGG AGTTGGACAA CTGCAAGCCG TGTCCTGTGG CCAAGCCGAC GTTCCTGTGC 
GGAGCCGACA ACAGGACCTA CTCATCACTA TGCAGATTGG ATTATCACAA TTGCATACAT 
TCGACGTCCA TACGGATCGC CTGCAAAGGA TTCTGTCCCT GCAAGGAGAT TGTCGATGGA 
AAGCGGCTGC AGCGCATCTC GGGCTACAAC AACAAGTACA ACAAGAAGAT CAGCCTGGAC 
CAGCAGCAGC AGCAGCAGCA ACAACAACAA CAGCAGCAGC AGCAGCAGCA GGCCTACAAG 
GACAGCAACA ACAACAACAT CATGATGAAC AGCGGCAACA TAATGGGCGG CAACAACAAC 
GACTTCAACA CGATCATGAA TGACAAAGAG GACAATAACC GGCACAACAA CCACATCAAT 
GCCCAGTACA CTTTCACGCC CGAGGAGATC AAATATGACA ACAAGCACTA CAAGTACCTC 
AAGTACACGG CCTACAAGAA GGACTCCAAG TACCAGGAGG ACAAGCACAA GATGCGCAAC 
TATAACGAGG TGGTCGAGAA GCAGCAGCAG AAGTTCAATA AGAACAGCAA CTTGAATGCC 
TATCCATCGA AATCGGCAGA GTGCAAGCCG CAGCAACTGA CGGCGATTGG CAACCGACTG 
CTGGACTGGT TCTCGGTGAT CATGGCGGAC AGCAAGAAGC GGCGCCAGCA CAGCCAGAAG 
TCAAAGGCCC ACTTCCCGCC CGCCTGCAAG ACGGAGGCCA AGTGGATGTT CGGCCACCTG 
GATCTGAATA ACGACGGGCA GCTGTCGCTG CAGGAGATGT ACGACCTGGA GCATGACCAG 
AACGAGCGTT GCATCAAGCC ATTCATCGAC ACCTGCGACT TGGACACGGA TAGCTCGATC 
AACACACGGG AATGGTGCAG ATGCTTCGAG AAGACGGATC GTCCATGTGC CGCTGTGCGC 
CGGAGAATCG CCGGCGACTT TGCAGGTGAG ATAGGAGCCT ACGCACCGGA CTGCGACATC 
CAGGGATTCT ACAAGCCTAC CCAGTGCCAC AATTCCGTGG GAGTTTGCTG GTGCGTGGAC 
AAGCACGGAG TGGAGTTCGC CAACACACGC ACACGCGGCA AACCCAATTG CGAGTCCGTG 
GTTAATAATG CCGCATCGCT CACATCCGAT GACGAGGACG AGGGCGCCGA TGACGAGGAC 
AGCGCGGAGG GCAGTGCCGA TCAGATGCTG GTCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cow cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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