Dcr-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dcr-1
Entrez Gene ID	42693
Full Name	Dicer-1
Synonyms	CG4792,DCR-1,DCR1,DICER,Dcr,Dcr1,Dicer,Dicer1,Dmel\CG4792,cg4792,dcr 1,dcr-1,dcr1,dicer,dicer-1,dicer1,dmDcr1
General protein information	Preferred Names Dicer-1 Names CG4792 gene product from transcript CG4792-RA CG4792-PA Dcr-1-PA dicer dicer 1 dicer1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 94C4-94C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_079729.3	NP_524453.1	Dicer-1

Pathways from BioSystems

Homologies

Homo sapiens (human)	DICER1	NP_085124.2
Rattus norvegicus (Norway rat)	Dicer1	XP_006240573.1
Danio rerio (zebrafish)	dicer1	NP_001154925.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002836	XP_003436256.1
Magnaporthe oryzae (rice blast fungus)	MGG_01541	XP_003714515.1
Neurospora crassa	NCU08270	XP_961898.1
	DICER1	XP_001100868.1
Gallus gallus (chicken)	DICER1	NP_001035555.1
Drosophila melanogaster (fruit fly)	Dcr-1	NP_524453.1
Caenorhabditis elegans (roundworm)	dcr-1	NP_498761.2
Xenopus tropicalis (tropical clawed frog)	dicer1	NP_001123390.2
Canis lupus familiaris (dog)	DICER1	XP_868526.3
Bos taurus (cattle)	DICER1	NP_976235.1
Schizosaccharomyces pombe (fission yeast)	dcr1	NP_588215.2
Pan troglodytes (chimpanzee)	DICER1	XP_001154369.1
Mus musculus (house mouse)	Dicer1	NP_683750.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

in both Dicer-1 and bantam depleted genetic backgrounds, Dacapo, an inhibitor of cyclin E-Cdk complex, was found to have elevated expression.
Title: Dicer-1 regulates proliferative potential of Drosophila larval neural stem cells through bantam miRNA based down-regulation of the G1/S inhibitor Dacapo.

Loqs may have roles in assisting the de novo emergence of miRNA genes by facilitating dicing of suboptimal hairpin substrates.
Title: The Drosophila Dicer-1 Partner Loquacious Enhances miRNA Processing from Hairpins with Unstable Structures at the Dicing Site.

[review] Dicer-1 lacks a functional DExD/H helicase domain and is responsible for processing hairpin-structured pre-microRNAs.
Title: Dicer's role as an antiviral: still an enigma.

Alternative splicing of the loquacious (loqs)mRNA generates three distinct Dicer partner proteins.
Title: Dicer partner proteins tune the length of mature miRNAs in flies and mammals.

Dcr-1 and Dap are required for nutrition-dependent cell cycle regulation in germline stem cells (GSCs) and reduction of dap partially rescues the cell cycle defect of Insulin receptor-deficient GSCs
Title: Dicer-1-dependent Dacapo suppression acts downstream of Insulin receptor in regulating cell division of Drosophila germline stem cells.

The following Dcr-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dcr-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08691	NM_079729.3 Latest version!	Drosophila melanogaster Dicer-1 (Dcr-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dcr-1 ( NM_079729.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524453.1 Drosophila melanogaster Dicer-1 (Dcr-1), mRNA.

Price & Availability↑

CloneID	OFa08691
Clone ID Related Accession (Same CDS sequence)	NM_079729.3
Accession Version	NM_079729.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Dicer-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079729.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGTTCC ACTGGTGCGA CAACAATCTG CACACCACCG TGTTCACGCC GCGCGACTTT 
CAGGTGGAGC TACTGGCCAC CGCCTACGAG CGGAACACGA TTATTTGCCT GGGCCATCGA 
AGTTCCAAGG AGTTTATAGC CCTCAAGCTG CTCCAGGAGC TGTCGCGTCG AGCACGCCGA 
CATGGTCGTG TCAGTGTCTA TCTCAGTTGC GAGGTTGGCA CCAGCACGGA ACCATGCTCC 
ATCTACACGA TGCTCACCCA CTTGACTGAC CTGCGGGTGT GGCAGGAGCA GCCGGATATG 
CAAATTCCCT TTGATCATTG CTGGACGGAC TATCACGTTT CCATCCTACG GCCAGAGGGA 
TTTCTTTATC TGCTCGAAAC TCGCGAGCTG CTGCTGAGCA GCGTCGAACT GATCGTGCTG 
GAAGATTGTC ATGACAGCGC CGTTTATCAG AGGATAAGGC CTCTGTTCGA GAATCACATT 
ATGCCAGCGC CACCGGCGGA CAGGCCACGG ATTCTCGGAC TCGCTGGACC GCTGCACAGC 
GCCGGATGTG AGCTGCAGCA ACTGAGCGCC ATGCTGGCCA CCCTGGAGCA GAGTGTGCTT 
TGCCAGATCG AGACGGCCAG TGATATTGTC ACCGTGTTGC GTTACTGTTC CCGACCGCAC 
GAATACATCG TACAGTGCGC CCCCTTCGAG ATGGACGAAC TGTCCCTGGT GCTTGCCGAT 
GTGCTCAACA CACACAAGTC CTTTTTATTG GACCACCGCT ACGATCCCTA CGAAATCTAC 
GGCACAGACC AGTTTATGGA CGAACTGAAA GACATACCCG ATCCCAAGGT GGACCCCCTG 
AACGTCATCA ACTCACTACT GGTCGTGCTG CACGAGATGG GTCCTTGGTG CACGCAGCGG 
GCTGCACATC ACTTTTACCA ATGCAATGAG AAGTTAAAGG TGAAGACGCC GCACGAACGT 
CACTACTTGC TGTACTGCCT AGTGAGCACG GCCCTTATCC AACTGTACTC CCTCTGCGAA 
CACGCATTCC ATCGACATTT AGGAAGTGGC AGCGATTCAC GCCAGACCAT CGAACGCTAT 
TCCAGCCCCA AGGTGCGACG TCTGTTGCAG ACACTGAGGT GCTTCAAGCC GGAAGAGGTG 
CACACCCAAG CGGACGGACT GCGCAGAATG CGGCATCAGG TGGATCAGGC GGACTTCAAT 
CGGTTATCTC ATACGCTGGA AAGCAAGTGC CGAATGGTGG ATCAAATGGA CCAACCGCCG 
ACGGAGACAC GAGCCCTGGT GGCCACTCTT GAGCAGATTC TGCACACGAC AGAGGACAGG 
CAGACGAACA GAAGCGCCGC TCGGGTGACT CCTACTCCTA CTCCCGCTCA TGCGAAGCCG 
AAACCTAGCT CTGGTGCCAA CACTGCACAA CCACGAACTC GTAGACGTGT GTACACCAGG 
CGCCACCACC GGGATCACAA TGATGGCAGC GACACGCTCT GCGCACTGAT TTACTGCAAC 
CAGAACCACA CGGCTCGCGT GCTCTTTGAG CTTCTAGCGG AGATTAGCAG ACGTGATCCC 
GATCTCAAGT TCCTACGCTG CCAGTACACC ACGGACCGGG TGGCAGATCC CACCACGGAG 
CCCAAAGAGG CTGAGTTGGA GCACCGGCGG CAGGAAGAGG TGCTAAAGCG CTTCCGCATG 
CATGACTGCA ATGTCCTGAT CGGTACTTCG GTGCTGGAAG AGGGCATCGA TGTGCCCAAG 
TGCAATTTGG TTGTGCGCTG GGATCCGCCA ACCACATATC GCAGTTACGT TCAGTGCAAA 
GGTCGAGCCC GTGCTGCTCC AGCCTATCAT GTCATTCTCG TCGCTCCGAG TTATAAAAGC 
CCAACTGTGG GGTCAGTGCA GCTGACCGAT CGGAGTCATC GGTATATTTG CGCGACTGGT 
GATACTACAG AGGCGGACAG CGACTCTGAT GATTCAGCGA TGCCAAACTC GTCCGGCTCG 
GATCCCTATA CTTTTGGCAC GGCACGCGGA ACCGTGAAGA TCCTCAACCC CGAAGTGTTC 
AGTAAACAAC CACCGACAGC GTGCGACATT AAGCTGCAGG AGATCCAGGA CGAATTGCCA 
GCCGCAGCGC AGCTGGATAC GAGCAACTCC AGCGACGAAG CCGTCAGCAT GAGTAACACG 
TCTCCAAGCG AGAGCAGTAC AGAACAAAAA TCCAGACGCT TCCAGTGCGA GCTGAGCTCT 
TTAACGGAGC CAGAAGACAC AAGTGATACT ACAGCCGAAA TCGATACTGC TCATAGTTTA 
GCCAGCACCA CGAAGGACTT GGTGCATCAA ATGGCACAGT ATCGCGAAAT CGAGCAGATG 
CTGCTATCCA AGTGCGCCAA CACAGAGCCG CCGGAGCAGG AGCAGAGTGA GGCGGAACGT 
TTTAGTGCCT GCCTGGCCGC ATACCGACCC AAGCCGCACC TGCTAACAGG CGCCTCCGTG 
GATCTGGGTT CTGCTATAGC TTTGGTCAAC AAGTACTGCG CCCGACTGCC AAGCGACACG 
TTCACCAAGT TGACGGCGTT GTGGCGCTGC ACCCGAAACG AAAGGGCTGG AGTGACCCTG 
TTTCAGTACA CACTCCGTCT GCCCATCAAC TCGCCATTGA AGCATGACAT TGTGGGTCTT 
CCGATGCCAA CTCAAACATT GGCCCGCCGA CTGGCTGCCT TGCAGGCTTG CGTGGAACTG 
CACAGGATCG GTGAGTTAGA CGATCAGTTG CAGCCTATCG GCAAGGAGGG ATTTCGTGCC 
CTGGAGCCGG ACTGGGAGTG CTTTGAACTG GAGCCAGAGG ACGAACAGAT TGTGCAGCTA 
AGCGATGAAC CACGTCCGGG AACAACGAAG CGTCGTCAGT ACTATTACAA ACGCATTGCA 
TCCGAATTTT GCGATTGCCG TCCCGTTGCC GGAGCGCCAT GCTATTTGTA CTTTATCCAA 
CTGACGCTCC AATGTCCGAT TCCCGAAGAG CAAAACACGC GGGGACGCAA GATTTATCCG 
CCCGAAGATG CGCAGCAGGG ATTCGGCATT CTAACCACCA AACGGATACC CAAGCTGAGT 
GCTTTCTCGA TATTCACGCG TTCCGGTGAG GTGAAGGTTT CCCTGGAGTT AGCTAAGGAA 
CGCGTGATTC TAACTAGCGA ACAAATAGTC TGCATCAACG GATTTTTAAA CTACACGTTC 
ACCAATGTAC TGCGTTTGCA AAAGTTTCTG ATGCTCTTCG ATCCGGACTC CACGGAAAAT 
TGTGTATTCA TTGTGCCCAC CGTGAAGGCA CCAGCTGGCG GCAAGCACAT CGACTGGCAG 
TTTCTGGAGC TGATCCAAGC GAATGGAAAT ACAATGCCAC GGGCAGTGCC CGATGAGGAG 
CGCCAGGCGC AGCCGTTTGA TCCGCAACGC TTCCAGGATG CCGTCGTTAT GCCGTGGTAT 
CGCAACCAGG ATCAACCGCA GTATTTCTAT GTGGCGGAGA TATGTCCACA TCTATCCCCA 
CTCAGCTGCT TCCCTGGTGA CAACTACCGC ACGTTCAAGC ACTACTACCT CGTCAAGTAT 
GGTCTGACCA TACAGAATAC CTCGCAGCCG CTATTGGACG TGGATCACAC CAGTGCGCGG 
TTAAACTTCC TCACGCCACG ATACGTTAAT CGCAAGGGCG TTGCTCTGCC CACTAGTTCG 
GAGGAGACAA AGCGGGCAAA GCGCGAGAAT CTCGAACAGA AGCAGATCCT TGTGCCAGAG 
CTCTGCACTG TGCATCCATT CCCCGCCTCC TTGTGGCGAA CTGCCGTGTG CCTGCCCTGC 
ATCCTGTACC GCATAAATGG TCTTCTATTG GCCGACGATA TTCGGAAACA GGTTTCTGCG 
GATCTGGGGC TGGGAAGGCA ACAGATCGAA GATGAGGATT TCGAGTGGCC CATGCTGGAC 
TTTGGGTGGA GTCTATCGGA GGTGCTCAAG AAATCGCGGG AGTCCAAACA AAAGGAGTCC 
CTTAAGGATG ATACTATTAA TGGCAAAGAC TTAGCTGATG TTGAAAAGAA ACCGACTAGC 
GAGGAGACCC AACTAGATAA GGATTCAAAA GACGATAAGG TTGAGAAAAG TGCTATTGAA 
CTAATCATTG AGGGAGAGGA GAAGCTGCAA GAGGCTGATG ACTTCATTGA GATAGGCACT 
TGGTCAAACG ATATGGCCGA CGATATAGCT AGTTTTAACC AAGAAGACGA CGACGAGGAT 
GACGCCTTCC ATCTCCCAGT TTTACCGGCA AACGTTAAGT TCTGTGATCA GCAAACGCGC 
TACGGTTCGC CCACATTTTG GGATGTGAGC AATGGCGAAA GCGGCTTCAA GGGTCCAAAG 
AGCAGTCAGA ATAAGCAGGG TGGCAAGGGC AAAGCAAAGG GTCCGGCAAA GCCCACATTT 
AACTATTATG ACTCGGACAA TTCGCTGGGT TCCAGCTACG ATGACGACGA TAACGCAGGT 
CCGCTCAATT ACATGCATCA CAACTACAGT TCGGATGACG ACGATGTGGC AGATGATATC 
GATGCGGGAC GCATTGCGTT CACCTCCAAG AATGAAGCGG AGACTATTGA AACCGCACAG 
GAAGTGGAAA AGCGCCAGAA GCAGCTGTCC ATCATCCAGG CGACCAATGC TAACGAGCGG 
CAGTATCAGC AGACAAAGAA CCTGCTCATT GGATTCAATT TTAAGCATGA GGACCAGAAG 
GAACCTGCCA CTATAAGATA TGAAGAATCC ATAGCTAAGC TCAAAACGGA AATAGAATCC 
GGCGGCATGT TGGTGCCGCA CGACCAGCAG TTGGTTCTAA AAAGAAGTGA TGCCGCTGAG 
GCTCAGGTTG CAAAGGTATC GATGATGGAG CTATTGAAGC AGCTGCTGCC GTATGTAAAT 
GAAGATGTGC TGGCCAAAAA GCTGGGTGAT AGGCGCGAGC TTCTGCTGTC GGATTTGGTA 
GAGCTAAATG CAGATTGGGT AGCGCGACAT GAGCAGGAGA CCTACAATGT AATGGGATGC 
GGAGATAGTT TTGACAACTA TAACGATCAT CATCGGCTGA ACTTGGATGA AAAGCAACTG 
AAACTGCAAT ACGAACGAAT TGAAATTGAG CCACCTACTT CCACGAAGGC CATAACCTCA 
GCCATATTAC CAGCTGGCTT CAGTTTCGAT CGACAACCGG ATCTAGTGGG CCATCCAGGA 
CCCAGTCCCA GCATCATTTT GCAAGCCCTC ACAATGTCCA ATGCTAACGA TGGCATCAAT 
CTGGAGCGAC TGGAGACAAT TGGAGATTCC TTTCTAAAGT ATGCCATTAC CACCTACTTG 
TACATCACCT ACGAGAATGT GCACGAGGGA AAACTAAGTC ACCTGCGCTC CAAGCAGGTT 
GCCAATCTCA ATCTCTATCG TCTGGGCAGA CGTAAGAGAC TGGGTGAATA TATGATAGCC 
ACTAAATTCG AGCCGCACGA CAATTGGCTG CCACCCTGCT ACTACGTGCC AAAGGAGCTA 
GAGAAGGCGC TCATCGAGGC GAAGATCCCC ACTCACCATT GGAAGCTGGC CGATCTGCTA 
GACATTAAGA ACCTAAGCAG TGTGCAAATC TGCGAGATGG TTCGCGAAAA AGCCGATGCC 
CTGGGCTTGG AGCAGAATGG GGGTGCCCAA AATGGACAAC TTGACGACTC CAATGATAGC 
TGCAATGATT TTAGCTGTTT TATTCCCTAC AACCTTGTTT CGCAACACAG CATTCCGGAT 
AAGTCTATTG CCGATTGCGT CGAAGCCCTC ATTGGAGCCT ATCTCATTGA GTGCGGACCC 
CGAGGGGCTT TACTCTTTAT GGCCTGGCTG GGCGTGAGAG TGCTCCCTAT CACAAGGCAG 
TTGGACGGGG GTAACCAGGA GCAACGAATA CCCGGTAGCA CAAAACCGAA TGCCGAAAAT 
GTGGTCACCG TTTACGGTGC ATGGCCCACG CCGCGTAGTC CACTGCTGCA CTTTGCTCCA 
AATGCTACGG AGGAGCTGGA CCAGTTACTA AGCGGCTTTG AGGAGTTTGA GGAGAGTTTG 
GGATACAAGT TCCGGGATCG GTCGTACCTG TTGCAAGCCA TGACACATGC CAGTTACACG 
CCCAATCGAT TGACGGATTG CTATCAGCGT CTGGAGTTCC TGGGCGATGC TGTTCTAGAT 
TACCTCATTA CGCGGCATTT ATACGAAGAT CCCCGCCAGC ATTCTCCAGG CGCATTAACG 
GATTTGCGGT CAGCACTGGT GAATAATACA ATATTCGCCT CCCTGGCTGT TCGCCATGGC 
TTCCACAAGT TCTTCCGGCA CCTCTCGCCG GGCCTTAACG ATGTGATTGA CCGTTTTGTG 
CGGATCCAGC AGGAGAATGG ACACTGCATC AGTGAGGAGT ACTACTTATT GTCCGAGGAG 
GAGTGCGATG ACGCCGAGGA CGTTGAGGTG CCCAAGGCAT TGGGCGACGT TTTCGAGTCG 
ATCGCAGGTG CCATTTTTCT CGACTCAAAC ATGTCGCTGG ACGTGGTTTG GCACGTATAT 
AGCAACATGA TGAGCCCGGA GATCGAGCAG TTCAGCAACT CAGTGCCAAA ATCGCCCATT 
CGGGAGCTCC TCGAGCTGGA GCCGGAAACC GCCAAGTTCG GCAAGCCCGA GAAGCTGGCG 
GATGGGCGAC GGGTGCGCGT TACCGTGGAT GTCTTCTGCA AAGGAACCTT CCGTGGCATC 
GGACGCAACT ATCGCATTGC CAAGTGCACG GCGGCCAAAT GCGCATTGCG CCAACTCAAA 
AAGCAGGGCT TGATAGCCAA AAAAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524453.1
CDS	62..6811
Translation	MAFHWCDNNLHTTVFTPRDFQVELLATAYERNTIICLGHRSSKEFIALKLLQELSRRARRHGRVSVYLSCEVGTSTEPCSIYTMLTHLTDLRVWQEQPDMQIPFDHCWTDYHVSILRPEGFLYLLETRELLLSSVELIVLEDCHDSAVYQRIRPLFENHIMPAPPADRPRILGLAGPLHSAGCELQQLSAMLATLEQSVLCQIETASDIVTVLRYCSRPHEYIVQCAPFEMDELSLVLADVLNTHKSFLLDHRYDPYEIYGTDQFMDELKDIPDPKVDPLNVINSLLVVLHEMGPWCTQRAAHHFYQCNEKLKVKTPHERHYLLYCLVSTALIQLYSLCEHAFHRHLGSGSDSRQTIERYSSPKVRRLLQTLRCFKPEEVHTQADGLRRMRHQVDQADFNRLSHTLESKCRMVDQMDQPPTETRALVATLEQILHTTEDRQTNRSAARVTPTPTPAHAKPKPSSGANTAQPRTRRRVYTRRHHRDHNDGSDTLCALIYCNQNHTARVLFELLAEISRRDPDLKFLRCQYTTDRVADPTTEPKEAELEHRRQEEVLKRFRMHDCNVLIGTSVLEEGIDVPKCNLVVRWDPPTTYRSYVQCKGRARAAPAYHVILVAPSYKSPTVGSVQLTDRSHRYICATGDTTEADSDSDDSAMPNSSGSDPYTFGTARGTVKILNPEVFSKQPPTACDIKLQEIQDELPAAAQLDTSNSSDEAVSMSNTSPSESSTEQKSRRFQCELSSLTEPEDTSDTTAEIDTAHSLASTTKDLVHQMAQYREIEQMLLSKCANTEPPEQEQSEAERFSACLAAYRPKPHLLTGASVDLGSAIALVNKYCARLPSDTFTKLTALWRCTRNERAGVTLFQYTLRLPINSPLKHDIVGLPMPTQTLARRLAALQACVELHRIGELDDQLQPIGKEGFRALEPDWECFELEPEDEQIVQLSDEPRPGTTKRRQYYYKRIASEFCDCRPVAGAPCYLYFIQLTLQCPIPEEQNTRGRKIYPPEDAQQGFGILTTKRIPKLSAFSIFTRSGEVKVSLELAKERVILTSEQIVCINGFLNYTFTNVLRLQKFLMLFDPDSTENCVFIVPTVKAPAGGKHIDWQFLELIQANGNTMPRAVPDEERQAQPFDPQRFQDAVVMPWYRNQDQPQYFYVAEICPHLSPLSCFPGDNYRTFKHYYLVKYGLTIQNTSQPLLDVDHTSARLNFLTPRYVNRKGVALPTSSEETKRAKRENLEQKQILVPELCTVHPFPASLWRTAVCLPCILYRINGLLLADDIRKQVSADLGLGRQQIEDEDFEWPMLDFGWSLSEVLKKSRESKQKESLKDDTINGKDLADVEKKPTSEETQLDKDSKDDKVEKSAIELIIEGEEKLQEADDFIEIGTWSNDMADDIASFNQEDDDEDDAFHLPVLPANVKFCDQQTRYGSPTFWDVSNGESGFKGPKSSQNKQGGKGKAKGPAKPTFNYYDSDNSLGSSYDDDDNAGPLNYMHHNYSSDDDDVADDIDAGRIAFTSKNEAETIETAQEVEKRQKQLSIIQATNANERQYQQTKNLLIGFNFKHEDQKEPATIRYEESIAKLKTEIESGGMLVPHDQQLVLKRSDAAEAQVAKVSMMELLKQLLPYVNEDVLAKKLGDRRELLLSDLVELNADWVARHEQETYNVMGCGDSFDNYNDHHRLNLDEKQLKLQYERIEIEPPTSTKAITSAILPAGFSFDRQPDLVGHPGPSPSIILQALTMSNANDGINLERLETIGDSFLKYAITTYLYITYENVHEGKLSHLRSKQVANLNLYRLGRRKRLGEYMIATKFEPHDNWLPPCYYVPKELEKALIEAKIPTHHWKLADLLDIKNLSSVQICEMVREKADALGLEQNGGAQNGQLDDSNDSCNDFSCFIPYNLVSQHSIPDKSIADCVEALIGAYLIECGPRGALLFMAWLGVRVLPITRQLDGGNQEQRIPGSTKPNAENVVTVYGAWPTPRSPLLHFAPNATEELDQLLSGFEEFEESLGYKFRDRSYLLQAMTHASYTPNRLTDCYQRLEFLGDAVLDYLITRHLYEDPRQHSPGALTDLRSALVNNTIFASLAVRHGFHKFFRHLSPGLNDVIDRFVRIQQENGHCISEEYYLLSEEECDDAEDVEVPKALGDVFESIAGAIFLDSNMSLDVVWHVYSNMMSPEIEQFSNSVPKSPIRELLELEPETAKFGKPEKLADGRRVRVTVDVFCKGTFRGIGRNYRIAKCTAAKCALRQLKKQGLIAKKD

Target ORF information:

RefSeq Version	NM_079729.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Dicer-1 (Dcr-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079729.3

ORF Insert Sequence:

ATGGCGTTCC ACTGGTGCGA CAACAATCTG CACACCACCG TGTTCACGCC GCGCGACTTT 
CAGGTGGAGC TACTGGCCAC CGCCTACGAG CGGAACACGA TTATTTGCCT GGGCCATCGA 
AGTTCCAAGG AGTTTATAGC CCTCAAGCTG CTCCAGGAGC TGTCGCGTCG AGCACGCCGA 
CATGGTCGTG TCAGTGTCTA TCTCAGTTGC GAGGTTGGCA CCAGCACGGA ACCATGCTCC 
ATCTACACGA TGCTCACCCA CTTGACTGAC CTGCGGGTGT GGCAGGAGCA GCCGGATATG 
CAAATTCCCT TTGATCATTG CTGGACGGAC TATCACGTTT CCATCCTACG GCCAGAGGGA 
TTTCTTTATC TGCTCGAAAC TCGCGAGCTG CTGCTGAGCA GCGTCGAACT GATCGTGCTG 
GAAGATTGTC ATGACAGCGC CGTTTATCAG AGGATAAGGC CTCTGTTCGA GAATCACATT 
ATGCCAGCGC CACCGGCGGA CAGGCCACGG ATTCTCGGAC TCGCTGGACC GCTGCACAGC 
GCCGGATGTG AGCTGCAGCA ACTGAGCGCC ATGCTGGCCA CCCTGGAGCA GAGTGTGCTT 
TGCCAGATCG AGACGGCCAG TGATATTGTC ACCGTGTTGC GTTACTGTTC CCGACCGCAC 
GAATACATCG TACAGTGCGC CCCCTTCGAG ATGGACGAAC TGTCCCTGGT GCTTGCCGAT 
GTGCTCAACA CACACAAGTC CTTTTTATTG GACCACCGCT ACGATCCCTA CGAAATCTAC 
GGCACAGACC AGTTTATGGA CGAACTGAAA GACATACCCG ATCCCAAGGT GGACCCCCTG 
AACGTCATCA ACTCACTACT GGTCGTGCTG CACGAGATGG GTCCTTGGTG CACGCAGCGG 
GCTGCACATC ACTTTTACCA ATGCAATGAG AAGTTAAAGG TGAAGACGCC GCACGAACGT 
CACTACTTGC TGTACTGCCT AGTGAGCACG GCCCTTATCC AACTGTACTC CCTCTGCGAA 
CACGCATTCC ATCGACATTT AGGAAGTGGC AGCGATTCAC GCCAGACCAT CGAACGCTAT 
TCCAGCCCCA AGGTGCGACG TCTGTTGCAG ACACTGAGGT GCTTCAAGCC GGAAGAGGTG 
CACACCCAAG CGGACGGACT GCGCAGAATG CGGCATCAGG TGGATCAGGC GGACTTCAAT 
CGGTTATCTC ATACGCTGGA AAGCAAGTGC CGAATGGTGG ATCAAATGGA CCAACCGCCG 
ACGGAGACAC GAGCCCTGGT GGCCACTCTT GAGCAGATTC TGCACACGAC AGAGGACAGG 
CAGACGAACA GAAGCGCCGC TCGGGTGACT CCTACTCCTA CTCCCGCTCA TGCGAAGCCG 
AAACCTAGCT CTGGTGCCAA CACTGCACAA CCACGAACTC GTAGACGTGT GTACACCAGG 
CGCCACCACC GGGATCACAA TGATGGCAGC GACACGCTCT GCGCACTGAT TTACTGCAAC 
CAGAACCACA CGGCTCGCGT GCTCTTTGAG CTTCTAGCGG AGATTAGCAG ACGTGATCCC 
GATCTCAAGT TCCTACGCTG CCAGTACACC ACGGACCGGG TGGCAGATCC CACCACGGAG 
CCCAAAGAGG CTGAGTTGGA GCACCGGCGG CAGGAAGAGG TGCTAAAGCG CTTCCGCATG 
CATGACTGCA ATGTCCTGAT CGGTACTTCG GTGCTGGAAG AGGGCATCGA TGTGCCCAAG 
TGCAATTTGG TTGTGCGCTG GGATCCGCCA ACCACATATC GCAGTTACGT TCAGTGCAAA 
GGTCGAGCCC GTGCTGCTCC AGCCTATCAT GTCATTCTCG TCGCTCCGAG TTATAAAAGC 
CCAACTGTGG GGTCAGTGCA GCTGACCGAT CGGAGTCATC GGTATATTTG CGCGACTGGT 
GATACTACAG AGGCGGACAG CGACTCTGAT GATTCAGCGA TGCCAAACTC GTCCGGCTCG 
GATCCCTATA CTTTTGGCAC GGCACGCGGA ACCGTGAAGA TCCTCAACCC CGAAGTGTTC 
AGTAAACAAC CACCGACAGC GTGCGACATT AAGCTGCAGG AGATCCAGGA CGAATTGCCA 
GCCGCAGCGC AGCTGGATAC GAGCAACTCC AGCGACGAAG CCGTCAGCAT GAGTAACACG 
TCTCCAAGCG AGAGCAGTAC AGAACAAAAA TCCAGACGCT TCCAGTGCGA GCTGAGCTCT 
TTAACGGAGC CAGAAGACAC AAGTGATACT ACAGCCGAAA TCGATACTGC TCATAGTTTA 
GCCAGCACCA CGAAGGACTT GGTGCATCAA ATGGCACAGT ATCGCGAAAT CGAGCAGATG 
CTGCTATCCA AGTGCGCCAA CACAGAGCCG CCGGAGCAGG AGCAGAGTGA GGCGGAACGT 
TTTAGTGCCT GCCTGGCCGC ATACCGACCC AAGCCGCACC TGCTAACAGG CGCCTCCGTG 
GATCTGGGTT CTGCTATAGC TTTGGTCAAC AAGTACTGCG CCCGACTGCC AAGCGACACG 
TTCACCAAGT TGACGGCGTT GTGGCGCTGC ACCCGAAACG AAAGGGCTGG AGTGACCCTG 
TTTCAGTACA CACTCCGTCT GCCCATCAAC TCGCCATTGA AGCATGACAT TGTGGGTCTT 
CCGATGCCAA CTCAAACATT GGCCCGCCGA CTGGCTGCCT TGCAGGCTTG CGTGGAACTG 
CACAGGATCG GTGAGTTAGA CGATCAGTTG CAGCCTATCG GCAAGGAGGG ATTTCGTGCC 
CTGGAGCCGG ACTGGGAGTG CTTTGAACTG GAGCCAGAGG ACGAACAGAT TGTGCAGCTA 
AGCGATGAAC CACGTCCGGG AACAACGAAG CGTCGTCAGT ACTATTACAA ACGCATTGCA 
TCCGAATTTT GCGATTGCCG TCCCGTTGCC GGAGCGCCAT GCTATTTGTA CTTTATCCAA 
CTGACGCTCC AATGTCCGAT TCCCGAAGAG CAAAACACGC GGGGACGCAA GATTTATCCG 
CCCGAAGATG CGCAGCAGGG ATTCGGCATT CTAACCACCA AACGGATACC CAAGCTGAGT 
GCTTTCTCGA TATTCACGCG TTCCGGTGAG GTGAAGGTTT CCCTGGAGTT AGCTAAGGAA 
CGCGTGATTC TAACTAGCGA ACAAATAGTC TGCATCAACG GATTTTTAAA CTACACGTTC 
ACCAATGTAC TGCGTTTGCA AAAGTTTCTG ATGCTCTTCG ATCCGGACTC CACGGAAAAT 
TGTGTATTCA TTGTGCCCAC CGTGAAGGCA CCAGCTGGCG GCAAGCACAT CGACTGGCAG 
TTTCTGGAGC TGATCCAAGC GAATGGAAAT ACAATGCCAC GGGCAGTGCC CGATGAGGAG 
CGCCAGGCGC AGCCGTTTGA TCCGCAACGC TTCCAGGATG CCGTCGTTAT GCCGTGGTAT 
CGCAACCAGG ATCAACCGCA GTATTTCTAT GTGGCGGAGA TATGTCCACA TCTATCCCCA 
CTCAGCTGCT TCCCTGGTGA CAACTACCGC ACGTTCAAGC ACTACTACCT CGTCAAGTAT 
GGTCTGACCA TACAGAATAC CTCGCAGCCG CTATTGGACG TGGATCACAC CAGTGCGCGG 
TTAAACTTCC TCACGCCACG ATACGTTAAT CGCAAGGGCG TTGCTCTGCC CACTAGTTCG 
GAGGAGACAA AGCGGGCAAA GCGCGAGAAT CTCGAACAGA AGCAGATCCT TGTGCCAGAG 
CTCTGCACTG TGCATCCATT CCCCGCCTCC TTGTGGCGAA CTGCCGTGTG CCTGCCCTGC 
ATCCTGTACC GCATAAATGG TCTTCTATTG GCCGACGATA TTCGGAAACA GGTTTCTGCG 
GATCTGGGGC TGGGAAGGCA ACAGATCGAA GATGAGGATT TCGAGTGGCC CATGCTGGAC 
TTTGGGTGGA GTCTATCGGA GGTGCTCAAG AAATCGCGGG AGTCCAAACA AAAGGAGTCC 
CTTAAGGATG ATACTATTAA TGGCAAAGAC TTAGCTGATG TTGAAAAGAA ACCGACTAGC 
GAGGAGACCC AACTAGATAA GGATTCAAAA GACGATAAGG TTGAGAAAAG TGCTATTGAA 
CTAATCATTG AGGGAGAGGA GAAGCTGCAA GAGGCTGATG ACTTCATTGA GATAGGCACT 
TGGTCAAACG ATATGGCCGA CGATATAGCT AGTTTTAACC AAGAAGACGA CGACGAGGAT 
GACGCCTTCC ATCTCCCAGT TTTACCGGCA AACGTTAAGT TCTGTGATCA GCAAACGCGC 
TACGGTTCGC CCACATTTTG GGATGTGAGC AATGGCGAAA GCGGCTTCAA GGGTCCAAAG 
AGCAGTCAGA ATAAGCAGGG TGGCAAGGGC AAAGCAAAGG GTCCGGCAAA GCCCACATTT 
AACTATTATG ACTCGGACAA TTCGCTGGGT TCCAGCTACG ATGACGACGA TAACGCAGGT 
CCGCTCAATT ACATGCATCA CAACTACAGT TCGGATGACG ACGATGTGGC AGATGATATC 
GATGCGGGAC GCATTGCGTT CACCTCCAAG AATGAAGCGG AGACTATTGA AACCGCACAG 
GAAGTGGAAA AGCGCCAGAA GCAGCTGTCC ATCATCCAGG CGACCAATGC TAACGAGCGG 
CAGTATCAGC AGACAAAGAA CCTGCTCATT GGATTCAATT TTAAGCATGA GGACCAGAAG 
GAACCTGCCA CTATAAGATA TGAAGAATCC ATAGCTAAGC TCAAAACGGA AATAGAATCC 
GGCGGCATGT TGGTGCCGCA CGACCAGCAG TTGGTTCTAA AAAGAAGTGA TGCCGCTGAG 
GCTCAGGTTG CAAAGGTATC GATGATGGAG CTATTGAAGC AGCTGCTGCC GTATGTAAAT 
GAAGATGTGC TGGCCAAAAA GCTGGGTGAT AGGCGCGAGC TTCTGCTGTC GGATTTGGTA 
GAGCTAAATG CAGATTGGGT AGCGCGACAT GAGCAGGAGA CCTACAATGT AATGGGATGC 
GGAGATAGTT TTGACAACTA TAACGATCAT CATCGGCTGA ACTTGGATGA AAAGCAACTG 
AAACTGCAAT ACGAACGAAT TGAAATTGAG CCACCTACTT CCACGAAGGC CATAACCTCA 
GCCATATTAC CAGCTGGCTT CAGTTTCGAT CGACAACCGG ATCTAGTGGG CCATCCAGGA 
CCCAGTCCCA GCATCATTTT GCAAGCCCTC ACAATGTCCA ATGCTAACGA TGGCATCAAT 
CTGGAGCGAC TGGAGACAAT TGGAGATTCC TTTCTAAAGT ATGCCATTAC CACCTACTTG 
TACATCACCT ACGAGAATGT GCACGAGGGA AAACTAAGTC ACCTGCGCTC CAAGCAGGTT 
GCCAATCTCA ATCTCTATCG TCTGGGCAGA CGTAAGAGAC TGGGTGAATA TATGATAGCC 
ACTAAATTCG AGCCGCACGA CAATTGGCTG CCACCCTGCT ACTACGTGCC AAAGGAGCTA 
GAGAAGGCGC TCATCGAGGC GAAGATCCCC ACTCACCATT GGAAGCTGGC CGATCTGCTA 
GACATTAAGA ACCTAAGCAG TGTGCAAATC TGCGAGATGG TTCGCGAAAA AGCCGATGCC 
CTGGGCTTGG AGCAGAATGG GGGTGCCCAA AATGGACAAC TTGACGACTC CAATGATAGC 
TGCAATGATT TTAGCTGTTT TATTCCCTAC AACCTTGTTT CGCAACACAG CATTCCGGAT 
AAGTCTATTG CCGATTGCGT CGAAGCCCTC ATTGGAGCCT ATCTCATTGA GTGCGGACCC 
CGAGGGGCTT TACTCTTTAT GGCCTGGCTG GGCGTGAGAG TGCTCCCTAT CACAAGGCAG 
TTGGACGGGG GTAACCAGGA GCAACGAATA CCCGGTAGCA CAAAACCGAA TGCCGAAAAT 
GTGGTCACCG TTTACGGTGC ATGGCCCACG CCGCGTAGTC CACTGCTGCA CTTTGCTCCA 
AATGCTACGG AGGAGCTGGA CCAGTTACTA AGCGGCTTTG AGGAGTTTGA GGAGAGTTTG 
GGATACAAGT TCCGGGATCG GTCGTACCTG TTGCAAGCCA TGACACATGC CAGTTACACG 
CCCAATCGAT TGACGGATTG CTATCAGCGT CTGGAGTTCC TGGGCGATGC TGTTCTAGAT 
TACCTCATTA CGCGGCATTT ATACGAAGAT CCCCGCCAGC ATTCTCCAGG CGCATTAACG 
GATTTGCGGT CAGCACTGGT GAATAATACA ATATTCGCCT CCCTGGCTGT TCGCCATGGC 
TTCCACAAGT TCTTCCGGCA CCTCTCGCCG GGCCTTAACG ATGTGATTGA CCGTTTTGTG 
CGGATCCAGC AGGAGAATGG ACACTGCATC AGTGAGGAGT ACTACTTATT GTCCGAGGAG 
GAGTGCGATG ACGCCGAGGA CGTTGAGGTG CCCAAGGCAT TGGGCGACGT TTTCGAGTCG 
ATCGCAGGTG CCATTTTTCT CGACTCAAAC ATGTCGCTGG ACGTGGTTTG GCACGTATAT 
AGCAACATGA TGAGCCCGGA GATCGAGCAG TTCAGCAACT CAGTGCCAAA ATCGCCCATT 
CGGGAGCTCC TCGAGCTGGA GCCGGAAACC GCCAAGTTCG GCAAGCCCGA GAAGCTGGCG 
GATGGGCGAC GGGTGCGCGT TACCGTGGAT GTCTTCTGCA AAGGAACCTT CCGTGGCATC 
GGACGCAACT ATCGCATTGC CAAGTGCACG GCGGCCAAAT GCGCATTGCG CCAACTCAAA 
AAGCAGGGCT TGATAGCCAA AAAAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Dcr-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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