mRRF2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mRRF2
Entrez Gene ID	42670
Full Name	Elongation Factor G2
Synonyms	CG31159,CG6943,Dmel\CG31159,dEF-G2
General protein information	Preferred Names Elongation Factor G2 Names CG31159 gene product from transcript CG31159-RA CG31159-PA CG31159-PB EF-G2-PA EF-G2-PB elongation factor G2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 94B6-94B6

mRNA and Protein(s)

mRNA	Protein	Name
NM_170028.3	NP_732771.2	mitochondrial ribosome recycling factor 2, isoform A
NM_001300539.1	NP_001287468.1	mitochondrial ribosome recycling factor 2, isoform B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GFM2	XP_005617535.1
Gallus gallus (chicken)	GFM2	XP_004937415.1
Caenorhabditis elegans (roundworm)	Y119D3B.14	NP_497345.2
Kluyveromyces lactis	KLLA0F13992g	XP_455710.1
Schizosaccharomyces pombe (fission yeast)	SPBC660.10	NP_595089.1
Xenopus tropicalis (tropical clawed frog)	gfm2	XP_004910454.1
Bos taurus (cattle)	GFM2	NP_001095579.1
Saccharomyces cerevisiae (baker's yeast)	MEF2	NP_012433.1
Magnaporthe oryzae (rice blast fungus)	MGG_01433	XP_003714382.1
Homo sapiens (human)	GFM2	NP_001268231.1
Mus musculus (house mouse)	Gfm2	NP_796240.3
Rattus norvegicus (Norway rat)	Gfm2	NP_001094135.1
Drosophila melanogaster (fruit fly)	EF-G2	NP_732771.2
Eremothecium gossypii	AGOS_AFR085W	NP_985632.1
	GFM2	XP_001102249.2
Danio rerio (zebrafish)	gfm2	NP_001071013.1
Anopheles gambiae (African malaria mosquito)	RRF2M_ANOGA	XP_317594.4
Neurospora crassa	NCU07021	XP_962160.2
Pan troglodytes (chimpanzee)	GFM2	XP_001150063.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mRRF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mRRF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa22135	NM_001300539.1 Latest version!	Drosophila melanogaster mitochondrial ribosome recycling factor 2 (mRRF2), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa08666	NM_170028.3 Latest version!	Drosophila melanogaster mitochondrial ribosome recycling factor 2 (mRRF2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mRRF2 ( NM_001300539.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287468.1 Drosophila melanogaster mitochondrial ribosome recycling factor 2 (mRRF2), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa22135
Clone ID Related Accession (Same CDS sequence)	NM_001300539.1
Accession Version	NM_001300539.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial ribosome recycling factor 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGAAAT ATGCATGGCA AAGCGGTCCA AAACAAAGCA ACAGGTGGCT ATGGCATCTT 
TCCAATCAAA TCTGGAAGCG TAGCTATAGC AGCAAGATCA GGAACATAGG CATTCTGGCG 
CATATCGATG CAGGCAAAAC AACGACAACG GAACGCATGC TGTTCTATGC GGGCAAGACC 
AGAGCTTTGG GTGAAGTGCA TCGCGGCAAC ACGGTCACTG ATTACCTAAC CCAGGAGCGC 
GAACGGGGCA TAACGATCTG CAGCTCGGCA GTAACATTTT CGTGGAATGA TCACCGCATC 
AACCTATTGG ACACACCCGG ACACATTGAC TTTACCATGG AGGTTGAGCA ATCTCTGTAT 
GCCGTGGATG GTGTGGTCGT TGTTCTGGAT GGCACAGCTG GCGTGGAGGC CCAAACGGTC 
ACCGTGTGGT CGCAGGCGGA CAAGCACAAA TTGCCACGTC TCATATTCGT CAACAAGATG 
GATCGTCCTG ATGCGGACTT TGAAAAGTGC GTTAGCGATC TGAAGGATAA GCTAGAAACG 
CAGCCCGTTT GCCTACAGTA CCCCGTGAAA AACGAAGATG GGGTTTTAGC CATCAATGAC 
GTGATCACCC TGGAGCGACT AAGCTGGCAG CAAAAGGACT TGGGCCGCAG TTACAGAAAT 
GTAAAACTGG AGCCCTCTGA TGATTTGCGC CTGCTGCAGG AAAAACGGAA TGAACTGATT 
GATCAACTGT CGGGACTAGA TGACGAATTG GCCGATGTGG TTATCAGCAC AGAAAGCTTT 
GACAATGTGG ACAATGCATT GATCGAAAGA GCTCTTCGAC GGGCAACCAC CCAGCAGAAG 
GTAGTGCCCG TATTGCTGGG CTCCGCCTAC AAAAATGTGG GCATACAGCG TTTAATGGAC 
GCTGTCAATG CTTACCTGCC TGCCCCCGAG GAACGAAACC AAATCTACGA CTGCTTTGGA 
ACAGAAGTGG CTGGCAAGGT GTTCAAAATT GTACACGATA AGCAACGTGG TCCATTAACC 
CTAGTTAGGA TTTTGAGAGG CGAAATAAAA CGAGGCATGC GTCTGATATC CGCTCGCGGA 
CAGGCTGAAG TTGTGTCTAA GCTGTATGAG CCATTGGCCG ACGAATACCG AGAGGTGAGC 
GCTGTGCAGT CGGGCGATGT GGTAATATGT GCAGGTCTGA AGTCCACGGT GACAGGAGAT 
TTGCTGACCT CAAGTCAGTC TGCGCTGAAG AATGCACAAA AGCGATATAA ACAAAGCCTG 
GGCAATACTG CGGCCAAAGT CGAGGAAGAT GACGAATTGG ATGAGTCAGA CGAGCTGTTT 
GCTATTGACC CGCAAATACC AGATGCCGTG TACTTCTGTT CGATCGAGCC TCCCAGTGTA 
TCCTCGCAAA CGGCCATGGA GCAGGCACTT AAACAGCTGC AACGGGAGGA TCCTAGTCTG 
CGTGTGAGCT ACGATTCAGT TACTGGACAG ACAGTACTCG GTGGCATGGG CGAGTTGCAC 
ATGGACATCA TCAAGTCGCG CATCCTGAGC GAGTACAAAA TCGACGTTGA CCTTGGTCCT 
CTACAAATTG CGTACAAGGA GACAATTGAA GCACCTGCGT TAACCACGCT AAGTGTGGAA 
AAGGAAATAG CCGGCAGCAA GCAGAGCGTC AGTATAACAC TGGAGGTTGT CAAGAATCAA 
GCCGAGTTGT TCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287468.1
CDS	79..1776
Translation	MLKYAWQSGPKQSNRWLWHLSNQIWKRSYSSKIRNIGILAHIDAGKTTTTERMLFYAGKTRALGEVHRGNTVTDYLTQERERGITICSSAVTFSWNDHRINLLDTPGHIDFTMEVEQSLYAVDGVVVVLDGTAGVEAQTVTVWSQADKHKLPRLIFVNKMDRPDADFEKCVSDLKDKLETQPVCLQYPVKNEDGVLAINDVITLERLSWQQKDLGRSYRNVKLEPSDDLRLLQEKRNELIDQLSGLDDELADVVISTESFDNVDNALIERALRRATTQQKVVPVLLGSAYKNVGIQRLMDAVNAYLPAPEERNQIYDCFGTEVAGKVFKIVHDKQRGPLTLVRILRGEIKRGMRLISARGQAEVVSKLYEPLADEYREVSAVQSGDVVICAGLKSTVTGDLLTSSQSALKNAQKRYKQSLGNTAAKVEEDDELDESDELFAIDPQIPDAVYFCSIEPPSVSSQTAMEQALKQLQREDPSLRVSYDSVTGQTVLGGMGELHMDIIKSRILSEYKIDVDLGPLQIAYKETIEAPALTTLSVEKEIAGSKQSVSITLEVVKNQAELFR

Target ORF information:

RefSeq Version	NM_001300539.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial ribosome recycling factor 2 (mRRF2), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300539.1

ORF Insert Sequence:

ATGCTGAAAT ATGCATGGCA AAGCGGTCCA AAACAAAGCA ACAGGTGGCT ATGGCATCTT 
TCCAATCAAA TCTGGAAGCG TAGCTATAGC AGCAAGATCA GGAACATAGG CATTCTGGCG 
CATATCGATG CAGGCAAAAC AACGACAACG GAACGCATGC TGTTCTATGC GGGCAAGACC 
AGAGCTTTGG GTGAAGTGCA TCGCGGCAAC ACGGTCACTG ATTACCTAAC CCAGGAGCGC 
GAACGGGGCA TAACGATCTG CAGCTCGGCA GTAACATTTT CGTGGAATGA TCACCGCATC 
AACCTATTGG ACACACCCGG ACACATTGAC TTTACCATGG AGGTTGAGCA ATCTCTGTAT 
GCCGTGGATG GTGTGGTCGT TGTTCTGGAT GGCACAGCTG GCGTGGAGGC CCAAACGGTC 
ACCGTGTGGT CGCAGGCGGA CAAGCACAAA TTGCCACGTC TCATATTCGT CAACAAGATG 
GATCGTCCTG ATGCGGACTT TGAAAAGTGC GTTAGCGATC TGAAGGATAA GCTAGAAACG 
CAGCCCGTTT GCCTACAGTA CCCCGTGAAA AACGAAGATG GGGTTTTAGC CATCAATGAC 
GTGATCACCC TGGAGCGACT AAGCTGGCAG CAAAAGGACT TGGGCCGCAG TTACAGAAAT 
GTAAAACTGG AGCCCTCTGA TGATTTGCGC CTGCTGCAGG AAAAACGGAA TGAACTGATT 
GATCAACTGT CGGGACTAGA TGACGAATTG GCCGATGTGG TTATCAGCAC AGAAAGCTTT 
GACAATGTGG ACAATGCATT GATCGAAAGA GCTCTTCGAC GGGCAACCAC CCAGCAGAAG 
GTAGTGCCCG TATTGCTGGG CTCCGCCTAC AAAAATGTGG GCATACAGCG TTTAATGGAC 
GCTGTCAATG CTTACCTGCC TGCCCCCGAG GAACGAAACC AAATCTACGA CTGCTTTGGA 
ACAGAAGTGG CTGGCAAGGT GTTCAAAATT GTACACGATA AGCAACGTGG TCCATTAACC 
CTAGTTAGGA TTTTGAGAGG CGAAATAAAA CGAGGCATGC GTCTGATATC CGCTCGCGGA 
CAGGCTGAAG TTGTGTCTAA GCTGTATGAG CCATTGGCCG ACGAATACCG AGAGGTGAGC 
GCTGTGCAGT CGGGCGATGT GGTAATATGT GCAGGTCTGA AGTCCACGGT GACAGGAGAT 
TTGCTGACCT CAAGTCAGTC TGCGCTGAAG AATGCACAAA AGCGATATAA ACAAAGCCTG 
GGCAATACTG CGGCCAAAGT CGAGGAAGAT GACGAATTGG ATGAGTCAGA CGAGCTGTTT 
GCTATTGACC CGCAAATACC AGATGCCGTG TACTTCTGTT CGATCGAGCC TCCCAGTGTA 
TCCTCGCAAA CGGCCATGGA GCAGGCACTT AAACAGCTGC AACGGGAGGA TCCTAGTCTG 
CGTGTGAGCT ACGATTCAGT TACTGGACAG ACAGTACTCG GTGGCATGGG CGAGTTGCAC 
ATGGACATCA TCAAGTCGCG CATCCTGAGC GAGTACAAAA TCGACGTTGA CCTTGGTCCT 
CTACAAATTG CGTACAAGGA GACAATTGAA GCACCTGCGT TAACCACGCT AAGTGTGGAA 
AAGGAAATAG CCGGCAGCAA GCAGAGCGTC AGTATAACAC TGGAGGTTGT CAAGAATCAA 
GCCGAGTTGT TCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mRRF2 ( NM_170028.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732771.2 Drosophila melanogaster mitochondrial ribosome recycling factor 2 (mRRF2), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa08666
Clone ID Related Accession (Same CDS sequence)	NM_170028.3
Accession Version	NM_170028.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mitochondrial ribosome recycling factor 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_170028.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTGAAAT ATGCATGGCA AAGCGGTCCA AAACAAAGCA ACAGGTGGCT ATGGCATCTT 
TCCAATCAAA TCTGGAAGCG TAGCTATAGC AGCAAGATCA GGAACATAGG CATTCTGGCG 
CATATCGATG CAGGCAAAAC AACGACAACG GAACGCATGC TGTTCTATGC GGGCAAGACC 
AGAGCTTTGG GTGAAGTGCA TCGCGGCAAC ACGGTCACTG ATTACCTAAC CCAGGAGCGC 
GAACGGGGCA TAACGATCTG CAGCTCGGCA GTAACATTTT CGTGGAATGA TCACCGCATC 
AACCTATTGG ACACACCCGG ACACATTGAC TTTACCATGG AGGTTGAGCA ATCTCTGTAT 
GCCGTGGATG GTGTGGTCGT TGTTCTGGAT GGCACAGCTG GCGTGGAGGC CCAAACGGTC 
ACCGTGTGGT CGCAGGCGGA CAAGCACAAA TTGCCACGTC TCATATTCGT CAACAAGATG 
GATCGTCCTG ATGCGGACTT TGAAAAGTGC GTTAGCGATC TGAAGGATAA GCTAGAAACG 
CAGCCCGTTT GCCTACAGTA CCCCGTGAAA AACGAAGATG GGGTTTTAGC CATCAATGAC 
GTGATCACCC TGGAGCGACT AAGCTGGCAG CAAAAGGACT TGGGCCGCAG TTACAGAAAT 
GTAAAACTGG AGCCCTCTGA TGATTTGCGC CTGCTGCAGG AAAAACGGAA TGAACTGATT 
GATCAACTGT CGGGACTAGA TGACGAATTG GCCGATGTGG TTATCAGCAC AGAAAGCTTT 
GACAATGTGG ACAATGCATT GATCGAAAGA GCTCTTCGAC GGGCAACCAC CCAGCAGAAG 
GTAGTGCCCG TATTGCTGGG CTCCGCCTAC AAAAATGTGG GCATACAGCG TTTAATGGAC 
GCTGTCAATG CTTACCTGCC TGCCCCCGAG GAACGAAACC AAATCTACGA CTGCTTTGGA 
ACAGAAGTGG CTGGCAAGGT GTTCAAAATT GTACACGATA AGCAACGTGG TCCATTAACC 
CTAGTTAGGA TTTTGAGAGG CGAAATAAAA CGAGGCATGC GTCTGATATC CGCTCGCGGA 
CAGGCTGAAG TTGTGTCTAA GCTGTATGAG CCATTGGCCG ACGAATACCG AGAGGTGAGC 
GCTGTGCAGT CGGGCGATGT GGTAATATGT GCAGGTCTGA AGTCCACGGT GACAGGAGAT 
TTGCTGACCT CAAGTCAGTC TGCGCTGAAG AATGCACAAA AGCGATATAA ACAAAGCCTG 
GGCAATACTG CGGCCAAAGT CGAGGAAGAT GACGAATTGG ATGAGTCAGA CGAGCTGTTT 
GCTATTGACC CGCAAATACC AGATGCCGTG TACTTCTGTT CGATCGAGCC TCCCAGTGTA 
TCCTCGCAAA CGGCCATGGA GCAGGCACTT AAACAGCTGC AACGGGAGGA TCCTAGTCTG 
CGTGTGAGCT ACGATTCAGT TACTGGACAG ACAGTACTCG GTGGCATGGG CGAGTTGCAC 
ATGGACATCA TCAAGTCGCG CATCCTGAGC GAGTACAAAA TCGACGTTGA CCTTGGTCCT 
CTACAAATTG CGTACAAGGA GACAATTGAA GCACCTGCGT TAACCACGCT AAGTGTGGAA 
AAGGAAATAG CCGGCAGCAA GCAGAGCGTC AGTATAACAC TGGAGGTTGT CAAGAATCAA 
GCCGAGTTGT TCAGTTTAGA TAAGTCACCA GATAACCTGC CAAATCTCAA CACATTGCGT 
CCACGAATTC TTCAAGTTCT GCGGAAGGGC TCCATCAGCG CTCTAGAGCG AGGACCTCGC 
GTTGGTGGGC AGGTTGTGGA GACTCAGATT CGTCTGCATA ACGCAACTAT TGGACGCGGC 
ACTGCTGATT CTTTTGTCAT GGCCACTGCA GCTCAGTGTG TGCAAAAGCT ACTGAGCACG 
AGTGGCACCA GGCTGTTGGA ACCCATTATG GCCCTTCAAA TTGTGGCGCC TAGCGAACGT 
ATTTCCGGAA TTATGGCGGA TCTTTCACGG CGACGAGCCC TGATTAACGA TGTCTTACCC 
AAAGGCGAAA GAAATAAGAT GATTCTGGTG AATGCTCCTT TGGCAGAACT CTCCGGCTAC 
TCGAGTGCCC TACGCACGAT AAGTTCCGGC ACAGCCAGCA TGACGATGCA ACCGTGTGGC 
TTCTCTTCAA TGAATTCCGT GGACGAATCG CTGGCGGAGC GCAGAGCACA GGGCCTTGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732771.2
CDS	79..2301
Translation	MLKYAWQSGPKQSNRWLWHLSNQIWKRSYSSKIRNIGILAHIDAGKTTTTERMLFYAGKTRALGEVHRGNTVTDYLTQERERGITICSSAVTFSWNDHRINLLDTPGHIDFTMEVEQSLYAVDGVVVVLDGTAGVEAQTVTVWSQADKHKLPRLIFVNKMDRPDADFEKCVSDLKDKLETQPVCLQYPVKNEDGVLAINDVITLERLSWQQKDLGRSYRNVKLEPSDDLRLLQEKRNELIDQLSGLDDELADVVISTESFDNVDNALIERALRRATTQQKVVPVLLGSAYKNVGIQRLMDAVNAYLPAPEERNQIYDCFGTEVAGKVFKIVHDKQRGPLTLVRILRGEIKRGMRLISARGQAEVVSKLYEPLADEYREVSAVQSGDVVICAGLKSTVTGDLLTSSQSALKNAQKRYKQSLGNTAAKVEEDDELDESDELFAIDPQIPDAVYFCSIEPPSVSSQTAMEQALKQLQREDPSLRVSYDSVTGQTVLGGMGELHMDIIKSRILSEYKIDVDLGPLQIAYKETIEAPALTTLSVEKEIAGSKQSVSITLEVVKNQAELFSLDKSPDNLPNLNTLRPRILQVLRKGSISALERGPRVGGQVVETQIRLHNATIGRGTADSFVMATAAQCVQKLLSTSGTRLLEPIMALQIVAPSERISGIMADLSRRRALINDVLPKGERNKMILVNAPLAELSGYSSALRTISSGTASMTMQPCGFSSMNSVDESLAERRAQGLE

Target ORF information:

RefSeq Version	NM_170028.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mitochondrial ribosome recycling factor 2 (mRRF2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170028.3

ORF Insert Sequence:

ATGCTGAAAT ATGCATGGCA AAGCGGTCCA AAACAAAGCA ACAGGTGGCT ATGGCATCTT 
TCCAATCAAA TCTGGAAGCG TAGCTATAGC AGCAAGATCA GGAACATAGG CATTCTGGCG 
CATATCGATG CAGGCAAAAC AACGACAACG GAACGCATGC TGTTCTATGC GGGCAAGACC 
AGAGCTTTGG GTGAAGTGCA TCGCGGCAAC ACGGTCACTG ATTACCTAAC CCAGGAGCGC 
GAACGGGGCA TAACGATCTG CAGCTCGGCA GTAACATTTT CGTGGAATGA TCACCGCATC 
AACCTATTGG ACACACCCGG ACACATTGAC TTTACCATGG AGGTTGAGCA ATCTCTGTAT 
GCCGTGGATG GTGTGGTCGT TGTTCTGGAT GGCACAGCTG GCGTGGAGGC CCAAACGGTC 
ACCGTGTGGT CGCAGGCGGA CAAGCACAAA TTGCCACGTC TCATATTCGT CAACAAGATG 
GATCGTCCTG ATGCGGACTT TGAAAAGTGC GTTAGCGATC TGAAGGATAA GCTAGAAACG 
CAGCCCGTTT GCCTACAGTA CCCCGTGAAA AACGAAGATG GGGTTTTAGC CATCAATGAC 
GTGATCACCC TGGAGCGACT AAGCTGGCAG CAAAAGGACT TGGGCCGCAG TTACAGAAAT 
GTAAAACTGG AGCCCTCTGA TGATTTGCGC CTGCTGCAGG AAAAACGGAA TGAACTGATT 
GATCAACTGT CGGGACTAGA TGACGAATTG GCCGATGTGG TTATCAGCAC AGAAAGCTTT 
GACAATGTGG ACAATGCATT GATCGAAAGA GCTCTTCGAC GGGCAACCAC CCAGCAGAAG 
GTAGTGCCCG TATTGCTGGG CTCCGCCTAC AAAAATGTGG GCATACAGCG TTTAATGGAC 
GCTGTCAATG CTTACCTGCC TGCCCCCGAG GAACGAAACC AAATCTACGA CTGCTTTGGA 
ACAGAAGTGG CTGGCAAGGT GTTCAAAATT GTACACGATA AGCAACGTGG TCCATTAACC 
CTAGTTAGGA TTTTGAGAGG CGAAATAAAA CGAGGCATGC GTCTGATATC CGCTCGCGGA 
CAGGCTGAAG TTGTGTCTAA GCTGTATGAG CCATTGGCCG ACGAATACCG AGAGGTGAGC 
GCTGTGCAGT CGGGCGATGT GGTAATATGT GCAGGTCTGA AGTCCACGGT GACAGGAGAT 
TTGCTGACCT CAAGTCAGTC TGCGCTGAAG AATGCACAAA AGCGATATAA ACAAAGCCTG 
GGCAATACTG CGGCCAAAGT CGAGGAAGAT GACGAATTGG ATGAGTCAGA CGAGCTGTTT 
GCTATTGACC CGCAAATACC AGATGCCGTG TACTTCTGTT CGATCGAGCC TCCCAGTGTA 
TCCTCGCAAA CGGCCATGGA GCAGGCACTT AAACAGCTGC AACGGGAGGA TCCTAGTCTG 
CGTGTGAGCT ACGATTCAGT TACTGGACAG ACAGTACTCG GTGGCATGGG CGAGTTGCAC 
ATGGACATCA TCAAGTCGCG CATCCTGAGC GAGTACAAAA TCGACGTTGA CCTTGGTCCT 
CTACAAATTG CGTACAAGGA GACAATTGAA GCACCTGCGT TAACCACGCT AAGTGTGGAA 
AAGGAAATAG CCGGCAGCAA GCAGAGCGTC AGTATAACAC TGGAGGTTGT CAAGAATCAA 
GCCGAGTTGT TCAGTTTAGA TAAGTCACCA GATAACCTGC CAAATCTCAA CACATTGCGT 
CCACGAATTC TTCAAGTTCT GCGGAAGGGC TCCATCAGCG CTCTAGAGCG AGGACCTCGC 
GTTGGTGGGC AGGTTGTGGA GACTCAGATT CGTCTGCATA ACGCAACTAT TGGACGCGGC 
ACTGCTGATT CTTTTGTCAT GGCCACTGCA GCTCAGTGTG TGCAAAAGCT ACTGAGCACG 
AGTGGCACCA GGCTGTTGGA ACCCATTATG GCCCTTCAAA TTGTGGCGCC TAGCGAACGT 
ATTTCCGGAA TTATGGCGGA TCTTTCACGG CGACGAGCCC TGATTAACGA TGTCTTACCC 
AAAGGCGAAA GAAATAAGAT GATTCTGGTG AATGCTCCTT TGGCAGAACT CTCCGGCTAC 
TCGAGTGCCC TACGCACGAT AAGTTCCGGC ACAGCCAGCA TGACGATGCA ACCGTGTGGC 
TTCTCTTCAA TGAATTCCGT GGACGAATCG CTGGCGGAGC GCAGAGCACA GGGCCTTGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mRRF2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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