CG12499 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG12499
Entrez Gene ID	42637
Full Name	CG12499 gene product from transcript CG12499-RA
Synonyms	Dmel\CG12499
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 94A14-94A14

mRNA and Protein(s)

mRNA	Protein	Name
NM_142787.2	NP_651044.1	uncharacterized protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG12499 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG12499 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05700	NM_142787.2 Latest version!	Drosophila melanogaster uncharacterized protein (CG12499), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG12499 ( NM_142787.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651044.1 Drosophila melanogaster uncharacterized protein (CG12499), mRNA.

Price & Availability↑

CloneID	OFa05700
Clone ID Related Accession (Same CDS sequence)	NM_142787.2
Accession Version	NM_142787.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142787.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCATTC AACCCAATCG AAGAAAGAGA AGGCACGAGG AGACAACTGC TGAGCAAGAG 
GATGAAGGTG AAGTGGAGGT GCCCAAAAAA CTGGCCAAGG AGTCCGACAC ACCGAAGAAA 
AAGAAAAAGA AGGATAAAAC TAAGGAATCA TTCGATGAAT CCACGCTGGA AAGCACTGAA 
ACCGATCCTG CTGACTCCAC TCCATCAAAG AAGAAGTCTA AAAAGACAAA GACATCCGAC 
ACAAGTGTAG AACAACAGGT GGAAGTGGAG ATGGCTGAGG ATGAAATAAG TCGCGATTCC 
GGGATTGATC CCGAAGAATC CCGCATACAC TTTCCCAACG ACTTTAAGAT GATGCACTTT 
CGAACCAAGC TTCGCGGCAA TAACTTCATA ACAGAACTGC GACACTTCCT GCACATGTGC 
TCAACCACGC GTCCTAAGCT TGCGGCTAAG TACATAGAAA AGCGTGGAAA GCCCTTGGAG 
TTAGCAGAAG CATTCGAAAG GATCGATAAG ACTAATTTAT TTCACGTGGA CTACCTCACA 
GAGGCTTTGC ACCGAGTTTT GATGGAAATA CTTACCAACC AGAAGAGCCA CATGGAGTCG 
GCTGTCCACG GGTGTAGATA TTTTCTCAAG GTTCACGGAG GAGTGCTCGA AAATCAAATG 
AAATCAATGC AGCTAGGCCA TCGGCGTAAT GCCCTAAAAC TGCTCACCGC TATTGTGTGC 
GTGGAGCCTC AATTGGCCCG CCAAGTTCTG AATTCGTATG ATGTGCTTTC CAACGTGACT 
GTGACGCAGA AATTGCTGTC CCATGAAAAA AGTGAATACC AATGCGAGGA TACAGTGCGC 
AAAGCGTTTA TTCTCTTTAT TTTGGCCTTC CTGGTCGAGG GCAATACTCT ACTTATCCGG 
AACATCCTGG ATCGAGGGCA ACTAATTGGT GTGCTGGCCA AGGGATTGGT TTACGATGAC 
CACGTAACCG TCTGCGTGGT GCTTAACGCA CTACGGCAAT ATGTGCTGGA GAACCCCGAA 
ATACAGAAGA CCAAAAAGGT TCTGGTTTTC GATTTAGAGT GCTGCAAAAA TCTGCGTCGC 
CTCTACGATT GGGAGGGACC CATGGGACTC AAGGTGATGT TTGCTAAATC GAAACATGTT 
TCTAAGATAC CTCCAAACCC ACAGGAGGCG GAAGCGGTAT CGGCTGCCGT TCACGAACTC 
CTGCTAGTCC TTTTGACTTC CCGCAAGCAC GGCATCTGCT TTGATGCGAT GACGCACTAC 
CGCCAAAAGC AAAACAAACT GCAGGGCAGA TTACTTGGCT CTCTATTCAA GCCCTACGAT 
AATCCGCGCA AAGTGGATTT GGTTATTCAA ACACTAACTG CTTGTCCAGA CTTGGGACGC 
AATACAGTGA GGAATTGTTC CTCTCTGCTC AGTCCGGTCA AAACCAAGCC GAAAGATTTG 
CCCATAGCTG TGGCGTTTCT CGCCCAAATC ATTAATGCAG TCCCGCCCAG TGCTCTATCA 
GCAGCATTCG ATAAGATGAC CCTAACGGAT TTCTCATTTT GGATCAAGGA GCTGTGTTTG 
CCCATCGAAG CTTTGCTTCA CATTACGGGT AAGAAGTACC TGAGCGATCC AGACTTCAAT 
TTGAGATTGG CTGTACTGCG TCTGCTGCTT TCCATGATGA AACAGTACAG CAACTATATG 
AATGCCATTG CTGTCCGAGA GCAATCAAAA AAAGCCGGCA ACTCGCTGCG TAAATTTAAG 
TTCGATTTAC TTAACCATCT GCTGGTGCAT TTTCCCACTA TCGAGTCCAT CCTATATTCG 
CTTTACATGT TCATCAAACA GAAAAACGTG GAAGGAGTGA ATGTACTTGA GAGTTTATCA 
ATCACTTTAG ATCTGGTGAT GCTACTCTGC AAGGAGAATC GAGCGTTTGT GAATAAGACA 
AGTCAGATTC TGGATTATTT GGATGTGTTG CGACCCCTAT ACGAAACTGA TTTTGACAAA 
CTGAATCAAC CGGAAGAGCT CTTGAAGATT CAATTAGAAC TGAAGTCGAT TAAAACCATC 
CTGCTGCTCT TTCCGCACTC CTTGAACCCA ACCCAACCGC TTTTCGGGAA CGTATTCAAA 
TCATTCATAA AAGCCTACAT GTTGGAGGAA AAATCAATCA AATCGGAGTC TGGAAACATA 
TTGAGTCGGC TGTTCCAAAA CACAGGGATG TTTGACTCCT GCAGGCATGA GATCAATATC 
TGGATAGAGG CCCTTATTGG ATTCGGTCCC GCTACGGTGA CCAACGTAGT AGAGATTCTG 
CTGACCATAC TCTGTTTGGA TTGGAGACAG ATTGAGCTTC CTGAACAAAG CGTTACGGAT 
ACTGCCATAA TCACCAAGAA CCTGAGCAAA TTGTTTGCTC AAATTGAGCA AGGTCAAACA 
GTGCAAGCTT ATGTGGAAAC TCTTACCCTT AGCAAGATGA TGCCGCTGTT GCTAAAGGGT 
GTAGAATTGG AGGAACCATA CGACGAATAT GTGGAGCTAG TATTTGTTCT GCTTTTCCAC 
TATCACAGCA AGCCGGAAAT AGTGCTGCAA ATGTACGAGG ATAACTTAAG CAAGCACACT 
AACTATATGA AGAGTTGGCT GCCTGGTGAA GGAAATGGTA AACCAAAGAA ACTTCCCCAC 
CTTATATTTG ATAAATGGCC CTTGCTTAAG CAAATGCGAA AGGTTAGCAC CGAAAGTGAA 
AACATGTCAT TCGAGCAGGT ATTTAAAATG GATGATACGG AAAACAACTT CGAGCTACAA 
CTAGAAGAGG GCCAGACAAT GTTGTTGCAT TCCTCCCTCG GAAATCCACG TCGCAATATG 
ATCTACGTCC AGGATTTGCT TTTCGTTTTT AGCCATTTCT TCACCAGCCA GAAATTAAGC 
AAACCTCAAG CAGAGCTGAC AGCAGATTTT CTTATTAAAT TCCTTCAAAT TGCCAGTCAG 
GTAGATGGGC CAGAAGAAGT TCAGGAGTCG GATGATGAGC AGCAGGACGA AAATTTTACT 
CAAAGTATGC TGCACTATAT ATTTAACCAT CGTCTGTGGC AATTGCACCC CACTGAATTG 
CTTAGTGAGA CGAGCGAATC TCGCTTGAAC TATGTTATCT TCCTCAGGAG ATTAATGGAG 
CATTGCCGCA GCTTGCCTCG ATTCGAGACC TATACTGCCA ACTATCGCTT GCACTTGATT 
AAGACCCTGG ATATTGCTTT GGATACACCG GTGGACCAGT TGGGAAATCA GCAGCAGCTC 
GACGAGTGTG TGAAATTGGT GGATTCATTT GAATTCAATT CTGATCAATG CTCTCAAATG 
CTGGACTTGC TAACAACCAA AGTGCAAGCA AAGGACTGCA TTCCGAATGG TAAACCAAAT 
CATTACTTTG ATCTACTTCT ACGTTTGCTG GGTCGATTGA ACACGTTAAA GGAGCCCATT 
GATTGCGGGA AGTCATTTAA ATTAATCCTG ACCTTCTACA AGGACTTCGG CGAGCTGAAT 
GAACAGCAAC TGGAACCATT TGAGTTGGCC GTCTTGGAGT TTTTGCTCAA TTACCATCAT 
CACATGGATG AGTGCGACAG TGAGTTCTTT GCCTGTTTCT TTACATCCTC TCGAAAACTC 
AGCAAATCCG CCATTCGTTT GGCCTGCTTG CTGCTCGAGC GCCAGCCCCG ATTAGCTGAA 
ACATTCGCAG AGTTACTGCC AAGCAATTTG GAGCAAAAGG ATTTGGTGTA CCCCCTCCTG 
GATATTGCTC TGACGAAGAA CTATCAGATA CCTCTACCAG TGCTTCAACG TTGCTATCAG 
ACCTACAAGA ACGGCTTCAT GAAGAGCATC GAGAAGCCAC AGAAGGCTGC CCTAATTTAC 
CGGGAGCATT CGGAGGCTAG CGCTCTTCTT ATTGCACTTT GCATGCCAAA GTCTGAGTGC 
TTCGATTACT GCCAGAAACA ACTGAAGCTC GATTCCGTGG AACTTTTCCA GGTGCGAGTC 
CTGCGTGAGA TTTACTGGCA GGGCTTTGCT GCCGCTAAGG ATGAAAAGCA AAAGGCCACC 
GTCTTTGTTA ATTATATAAA CCTTAATGTT CAACTGTTAG CACTGGATTT GAAGAAACAG 
GCTTTGGATC TGCCTAAGCT AGAGCAAATC TCGTGGAACT GTTACGAGTG GTGGAAGACG 
AATCGGACCG ACGATCTACC CTTGGACTGG AGTCGCTTGC AAAAGAACCC GCAGTGGCTT 
AACTTTTGCA AGAGTAGCTT AAAGTTGGCC CTTCACCTCG GCGAGGAACG ATTGCCACTT 
CAGGATGAAA CAAATGGGCT TGTTCTAAAG CTGATGGCCT TCCTGTGTGA TGGAATGTAT 
GATGAATATA AGGAGGATGC ACAGCAGGAG GAGGATGCAA CACCAACACT TCTATACGAA 
ATGATTTGCA CGCACTCCTG CTGTTTTGAC CATCTCTTAG GCAAGCCAAG TGAAACCAAA 
ACGCACATTT TACACCTAAT GGAGGTGTTG GCCAGAAAGG CTCCTAGTGC CCTGCAATCT 
GATCACATAC CCGTAATCCT TGGCTCCTAT CATGCAAAAC TGTCACCTGC GGATCGATAT 
GGATTAGCAC TGCTGGAGCA CTACGAACGC TTTGACGTGG GCTTGGATAA GTTTAGACCT 
TTCATTTGGG GCGAAAGTGC TGTGGCCCAT TATGCCCTGA GGGCTGCGGA CGAGGATGTG 
CGAACCAGGC TGCAACAGCA GGAGACGAAT GTGGCTCAGG TGCTAGCCCT GATTGTTCGA 
GAGACTAGCG AGTACACCAT TGAAAATTTC CCGATTTGGA GAAGCCTCCA TGCTAGCCAA 
CAACTACCCT CCACGGAGTT TCAAAATCCT GCTAAGAAAT CCATGAGCTT TGGCGACAAT 
CAGTTGGAGC AAATGGTGGA GCAGGGTCAG CTAGATTTCC CACAGGATTT ACGACGAATT 
TGCCCGAAGC GCGAGACTAT ATACGAGACC TGCTACGATC CCGCCTTCCT GTTACCCCTG 
ATGATGCACT GCTTTGCACC CGAGTCCGTA GTGTGGGCCC GTTGGCCTGT CCAGAATGGA 
TTACTGGCGA TCACCTTCTG CGGTCTATCA TCTCTGGACA AGGACATGAG ACTGGCGGCT 
GCCAGTTGCC AGCAACGCTA TCGTGCTCAT TTCGAGCTCT CCAATTTCTA CGAGCGTCCG 
CTGTGGACGC AGGCCTATGA GAACATTCAG GCAGGATTGG CTGATCTGAG AAACTCCTGG 
CTGGCACAGA GGAGAACGCA TGGCGGAACG CCTAGAGTTC CTTTGATTCC TGGCCTGTTT 
ATCGCTCACA GCTTTAATTT GAGCACAGAG CCAACGCATT TGCTGTACAA GCGACTGATG 
ATGTATTTGC GGCTGAAACA GAGCTTCAAC TTTCAAACGA TACCCGACTT CAATGTTTTC 
TTCTACTCCC AGGAACCAGA GCACCAGCAG TACCGCGAGT TTATCGTGGA GTTGCTTAGA 
TGCGGCATTA AGTGCAGCGC CGACCTTTTC CTGCTGGTAT CCACCAATAC GTTCAAGGTC 
CTCCTTGGAT TCTACGGCTC CAGTTTGGCC AGTTTGGAAA CGAATCTGCT GCTGCTTTCG 
GTGCTCTCCA CATGCGTCAA AATTCCCGGA GCTGTGAAAA TCATGCTGGA GCACGTAGGC 
ATCCTGTCCT GGCTGGTGGG CGTAATCCGC GACACAGAGT TCCATCAGTT CGATATCATC 
GAGGGTGTCA TCAGCATCCT CAACAATCTG TGGTATGCCT TGGCCGCGTC CCGTTCGGAA 
CTGACGGATT ACGACCACAT CCAGTTGCGA GTTTACCGTC TGGTGTTGCA GCTACTGCCG 
CTGCTCTCTC CACGGATATC AGTTCCTGGC TTGGGTCGCC TGTTCAACGT TCTGTGGAAA 
ACTGGCTCGG CCTGTGGCCA GCATCGTGCC GTGTCCGCCA CCCAAATGGA TACCCTGCTG 
GAGTGCGTTT CACGCCAATG GCCCGATCAG CTGGCGAGTG TGCGTTATGT ACGGAGCTTT 
GGCAGCGCTG GCGCCTGTTC GCGTTCCGAA TACTGCGACT GGCTGGCCAA GGATCAAAAG 
CTGGAGACTC CTGCCGTCCT CGCGTTGTCC AGTCTGCGGG AATATGTCAT TGACTGGGCG 
CAAGCGCATA AATCGGAGAG TGACGGGGAA ACCAAGCTGC TGGTGGAACC AACGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651044.1
CDS	56..6235
Translation	MAIQPNRRKRRHEETTAEQEDEGEVEVPKKLAKESDTPKKKKKKDKTKESFDESTLESTETDPADSTPSKKKSKKTKTSDTSVEQQVEVEMAEDEISRDSGIDPEESRIHFPNDFKMMHFRTKLRGNNFITELRHFLHMCSTTRPKLAAKYIEKRGKPLELAEAFERIDKTNLFHVDYLTEALHRVLMEILTNQKSHMESAVHGCRYFLKVHGGVLENQMKSMQLGHRRNALKLLTAIVCVEPQLARQVLNSYDVLSNVTVTQKLLSHEKSEYQCEDTVRKAFILFILAFLVEGNTLLIRNILDRGQLIGVLAKGLVYDDHVTVCVVLNALRQYVLENPEIQKTKKVLVFDLECCKNLRRLYDWEGPMGLKVMFAKSKHVSKIPPNPQEAEAVSAAVHELLLVLLTSRKHGICFDAMTHYRQKQNKLQGRLLGSLFKPYDNPRKVDLVIQTLTACPDLGRNTVRNCSSLLSPVKTKPKDLPIAVAFLAQIINAVPPSALSAAFDKMTLTDFSFWIKELCLPIEALLHITGKKYLSDPDFNLRLAVLRLLLSMMKQYSNYMNAIAVREQSKKAGNSLRKFKFDLLNHLLVHFPTIESILYSLYMFIKQKNVEGVNVLESLSITLDLVMLLCKENRAFVNKTSQILDYLDVLRPLYETDFDKLNQPEELLKIQLELKSIKTILLLFPHSLNPTQPLFGNVFKSFIKAYMLEEKSIKSESGNILSRLFQNTGMFDSCRHEINIWIEALIGFGPATVTNVVEILLTILCLDWRQIELPEQSVTDTAIITKNLSKLFAQIEQGQTVQAYVETLTLSKMMPLLLKGVELEEPYDEYVELVFVLLFHYHSKPEIVLQMYEDNLSKHTNYMKSWLPGEGNGKPKKLPHLIFDKWPLLKQMRKVSTESENMSFEQVFKMDDTENNFELQLEEGQTMLLHSSLGNPRRNMIYVQDLLFVFSHFFTSQKLSKPQAELTADFLIKFLQIASQVDGPEEVQESDDEQQDENFTQSMLHYIFNHRLWQLHPTELLSETSESRLNYVIFLRRLMEHCRSLPRFETYTANYRLHLIKTLDIALDTPVDQLGNQQQLDECVKLVDSFEFNSDQCSQMLDLLTTKVQAKDCIPNGKPNHYFDLLLRLLGRLNTLKEPIDCGKSFKLILTFYKDFGELNEQQLEPFELAVLEFLLNYHHHMDECDSEFFACFFTSSRKLSKSAIRLACLLLERQPRLAETFAELLPSNLEQKDLVYPLLDIALTKNYQIPLPVLQRCYQTYKNGFMKSIEKPQKAALIYREHSEASALLIALCMPKSECFDYCQKQLKLDSVELFQVRVLREIYWQGFAAAKDEKQKATVFVNYINLNVQLLALDLKKQALDLPKLEQISWNCYEWWKTNRTDDLPLDWSRLQKNPQWLNFCKSSLKLALHLGEERLPLQDETNGLVLKLMAFLCDGMYDEYKEDAQQEEDATPTLLYEMICTHSCCFDHLLGKPSETKTHILHLMEVLARKAPSALQSDHIPVILGSYHAKLSPADRYGLALLEHYERFDVGLDKFRPFIWGESAVAHYALRAADEDVRTRLQQQETNVAQVLALIVRETSEYTIENFPIWRSLHASQQLPSTEFQNPAKKSMSFGDNQLEQMVEQGQLDFPQDLRRICPKRETIYETCYDPAFLLPLMMHCFAPESVVWARWPVQNGLLAITFCGLSSLDKDMRLAAASCQQRYRAHFELSNFYERPLWTQAYENIQAGLADLRNSWLAQRRTHGGTPRVPLIPGLFIAHSFNLSTEPTHLLYKRLMMYLRLKQSFNFQTIPDFNVFFYSQEPEHQQYREFIVELLRCGIKCSADLFLLVSTNTFKVLLGFYGSSLASLETNLLLLSVLSTCVKIPGAVKIMLEHVGILSWLVGVIRDTEFHQFDIIEGVISILNNLWYALAASRSELTDYDHIQLRVYRLVLQLLPLLSPRISVPGLGRLFNVLWKTGSACGQHRAVSATQMDTLLECVSRQWPDQLASVRYVRSFGSAGACSRSEYCDWLAKDQKLETPAVLALSSLREYVIDWAQAHKSESDGETKLLVEPTE

Target ORF information:

RefSeq Version	NM_142787.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein (CG12499), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142787.2

ORF Insert Sequence:

ATGGCCATTC AACCCAATCG AAGAAAGAGA AGGCACGAGG AGACAACTGC TGAGCAAGAG 
GATGAAGGTG AAGTGGAGGT GCCCAAAAAA CTGGCCAAGG AGTCCGACAC ACCGAAGAAA 
AAGAAAAAGA AGGATAAAAC TAAGGAATCA TTCGATGAAT CCACGCTGGA AAGCACTGAA 
ACCGATCCTG CTGACTCCAC TCCATCAAAG AAGAAGTCTA AAAAGACAAA GACATCCGAC 
ACAAGTGTAG AACAACAGGT GGAAGTGGAG ATGGCTGAGG ATGAAATAAG TCGCGATTCC 
GGGATTGATC CCGAAGAATC CCGCATACAC TTTCCCAACG ACTTTAAGAT GATGCACTTT 
CGAACCAAGC TTCGCGGCAA TAACTTCATA ACAGAACTGC GACACTTCCT GCACATGTGC 
TCAACCACGC GTCCTAAGCT TGCGGCTAAG TACATAGAAA AGCGTGGAAA GCCCTTGGAG 
TTAGCAGAAG CATTCGAAAG GATCGATAAG ACTAATTTAT TTCACGTGGA CTACCTCACA 
GAGGCTTTGC ACCGAGTTTT GATGGAAATA CTTACCAACC AGAAGAGCCA CATGGAGTCG 
GCTGTCCACG GGTGTAGATA TTTTCTCAAG GTTCACGGAG GAGTGCTCGA AAATCAAATG 
AAATCAATGC AGCTAGGCCA TCGGCGTAAT GCCCTAAAAC TGCTCACCGC TATTGTGTGC 
GTGGAGCCTC AATTGGCCCG CCAAGTTCTG AATTCGTATG ATGTGCTTTC CAACGTGACT 
GTGACGCAGA AATTGCTGTC CCATGAAAAA AGTGAATACC AATGCGAGGA TACAGTGCGC 
AAAGCGTTTA TTCTCTTTAT TTTGGCCTTC CTGGTCGAGG GCAATACTCT ACTTATCCGG 
AACATCCTGG ATCGAGGGCA ACTAATTGGT GTGCTGGCCA AGGGATTGGT TTACGATGAC 
CACGTAACCG TCTGCGTGGT GCTTAACGCA CTACGGCAAT ATGTGCTGGA GAACCCCGAA 
ATACAGAAGA CCAAAAAGGT TCTGGTTTTC GATTTAGAGT GCTGCAAAAA TCTGCGTCGC 
CTCTACGATT GGGAGGGACC CATGGGACTC AAGGTGATGT TTGCTAAATC GAAACATGTT 
TCTAAGATAC CTCCAAACCC ACAGGAGGCG GAAGCGGTAT CGGCTGCCGT TCACGAACTC 
CTGCTAGTCC TTTTGACTTC CCGCAAGCAC GGCATCTGCT TTGATGCGAT GACGCACTAC 
CGCCAAAAGC AAAACAAACT GCAGGGCAGA TTACTTGGCT CTCTATTCAA GCCCTACGAT 
AATCCGCGCA AAGTGGATTT GGTTATTCAA ACACTAACTG CTTGTCCAGA CTTGGGACGC 
AATACAGTGA GGAATTGTTC CTCTCTGCTC AGTCCGGTCA AAACCAAGCC GAAAGATTTG 
CCCATAGCTG TGGCGTTTCT CGCCCAAATC ATTAATGCAG TCCCGCCCAG TGCTCTATCA 
GCAGCATTCG ATAAGATGAC CCTAACGGAT TTCTCATTTT GGATCAAGGA GCTGTGTTTG 
CCCATCGAAG CTTTGCTTCA CATTACGGGT AAGAAGTACC TGAGCGATCC AGACTTCAAT 
TTGAGATTGG CTGTACTGCG TCTGCTGCTT TCCATGATGA AACAGTACAG CAACTATATG 
AATGCCATTG CTGTCCGAGA GCAATCAAAA AAAGCCGGCA ACTCGCTGCG TAAATTTAAG 
TTCGATTTAC TTAACCATCT GCTGGTGCAT TTTCCCACTA TCGAGTCCAT CCTATATTCG 
CTTTACATGT TCATCAAACA GAAAAACGTG GAAGGAGTGA ATGTACTTGA GAGTTTATCA 
ATCACTTTAG ATCTGGTGAT GCTACTCTGC AAGGAGAATC GAGCGTTTGT GAATAAGACA 
AGTCAGATTC TGGATTATTT GGATGTGTTG CGACCCCTAT ACGAAACTGA TTTTGACAAA 
CTGAATCAAC CGGAAGAGCT CTTGAAGATT CAATTAGAAC TGAAGTCGAT TAAAACCATC 
CTGCTGCTCT TTCCGCACTC CTTGAACCCA ACCCAACCGC TTTTCGGGAA CGTATTCAAA 
TCATTCATAA AAGCCTACAT GTTGGAGGAA AAATCAATCA AATCGGAGTC TGGAAACATA 
TTGAGTCGGC TGTTCCAAAA CACAGGGATG TTTGACTCCT GCAGGCATGA GATCAATATC 
TGGATAGAGG CCCTTATTGG ATTCGGTCCC GCTACGGTGA CCAACGTAGT AGAGATTCTG 
CTGACCATAC TCTGTTTGGA TTGGAGACAG ATTGAGCTTC CTGAACAAAG CGTTACGGAT 
ACTGCCATAA TCACCAAGAA CCTGAGCAAA TTGTTTGCTC AAATTGAGCA AGGTCAAACA 
GTGCAAGCTT ATGTGGAAAC TCTTACCCTT AGCAAGATGA TGCCGCTGTT GCTAAAGGGT 
GTAGAATTGG AGGAACCATA CGACGAATAT GTGGAGCTAG TATTTGTTCT GCTTTTCCAC 
TATCACAGCA AGCCGGAAAT AGTGCTGCAA ATGTACGAGG ATAACTTAAG CAAGCACACT 
AACTATATGA AGAGTTGGCT GCCTGGTGAA GGAAATGGTA AACCAAAGAA ACTTCCCCAC 
CTTATATTTG ATAAATGGCC CTTGCTTAAG CAAATGCGAA AGGTTAGCAC CGAAAGTGAA 
AACATGTCAT TCGAGCAGGT ATTTAAAATG GATGATACGG AAAACAACTT CGAGCTACAA 
CTAGAAGAGG GCCAGACAAT GTTGTTGCAT TCCTCCCTCG GAAATCCACG TCGCAATATG 
ATCTACGTCC AGGATTTGCT TTTCGTTTTT AGCCATTTCT TCACCAGCCA GAAATTAAGC 
AAACCTCAAG CAGAGCTGAC AGCAGATTTT CTTATTAAAT TCCTTCAAAT TGCCAGTCAG 
GTAGATGGGC CAGAAGAAGT TCAGGAGTCG GATGATGAGC AGCAGGACGA AAATTTTACT 
CAAAGTATGC TGCACTATAT ATTTAACCAT CGTCTGTGGC AATTGCACCC CACTGAATTG 
CTTAGTGAGA CGAGCGAATC TCGCTTGAAC TATGTTATCT TCCTCAGGAG ATTAATGGAG 
CATTGCCGCA GCTTGCCTCG ATTCGAGACC TATACTGCCA ACTATCGCTT GCACTTGATT 
AAGACCCTGG ATATTGCTTT GGATACACCG GTGGACCAGT TGGGAAATCA GCAGCAGCTC 
GACGAGTGTG TGAAATTGGT GGATTCATTT GAATTCAATT CTGATCAATG CTCTCAAATG 
CTGGACTTGC TAACAACCAA AGTGCAAGCA AAGGACTGCA TTCCGAATGG TAAACCAAAT 
CATTACTTTG ATCTACTTCT ACGTTTGCTG GGTCGATTGA ACACGTTAAA GGAGCCCATT 
GATTGCGGGA AGTCATTTAA ATTAATCCTG ACCTTCTACA AGGACTTCGG CGAGCTGAAT 
GAACAGCAAC TGGAACCATT TGAGTTGGCC GTCTTGGAGT TTTTGCTCAA TTACCATCAT 
CACATGGATG AGTGCGACAG TGAGTTCTTT GCCTGTTTCT TTACATCCTC TCGAAAACTC 
AGCAAATCCG CCATTCGTTT GGCCTGCTTG CTGCTCGAGC GCCAGCCCCG ATTAGCTGAA 
ACATTCGCAG AGTTACTGCC AAGCAATTTG GAGCAAAAGG ATTTGGTGTA CCCCCTCCTG 
GATATTGCTC TGACGAAGAA CTATCAGATA CCTCTACCAG TGCTTCAACG TTGCTATCAG 
ACCTACAAGA ACGGCTTCAT GAAGAGCATC GAGAAGCCAC AGAAGGCTGC CCTAATTTAC 
CGGGAGCATT CGGAGGCTAG CGCTCTTCTT ATTGCACTTT GCATGCCAAA GTCTGAGTGC 
TTCGATTACT GCCAGAAACA ACTGAAGCTC GATTCCGTGG AACTTTTCCA GGTGCGAGTC 
CTGCGTGAGA TTTACTGGCA GGGCTTTGCT GCCGCTAAGG ATGAAAAGCA AAAGGCCACC 
GTCTTTGTTA ATTATATAAA CCTTAATGTT CAACTGTTAG CACTGGATTT GAAGAAACAG 
GCTTTGGATC TGCCTAAGCT AGAGCAAATC TCGTGGAACT GTTACGAGTG GTGGAAGACG 
AATCGGACCG ACGATCTACC CTTGGACTGG AGTCGCTTGC AAAAGAACCC GCAGTGGCTT 
AACTTTTGCA AGAGTAGCTT AAAGTTGGCC CTTCACCTCG GCGAGGAACG ATTGCCACTT 
CAGGATGAAA CAAATGGGCT TGTTCTAAAG CTGATGGCCT TCCTGTGTGA TGGAATGTAT 
GATGAATATA AGGAGGATGC ACAGCAGGAG GAGGATGCAA CACCAACACT TCTATACGAA 
ATGATTTGCA CGCACTCCTG CTGTTTTGAC CATCTCTTAG GCAAGCCAAG TGAAACCAAA 
ACGCACATTT TACACCTAAT GGAGGTGTTG GCCAGAAAGG CTCCTAGTGC CCTGCAATCT 
GATCACATAC CCGTAATCCT TGGCTCCTAT CATGCAAAAC TGTCACCTGC GGATCGATAT 
GGATTAGCAC TGCTGGAGCA CTACGAACGC TTTGACGTGG GCTTGGATAA GTTTAGACCT 
TTCATTTGGG GCGAAAGTGC TGTGGCCCAT TATGCCCTGA GGGCTGCGGA CGAGGATGTG 
CGAACCAGGC TGCAACAGCA GGAGACGAAT GTGGCTCAGG TGCTAGCCCT GATTGTTCGA 
GAGACTAGCG AGTACACCAT TGAAAATTTC CCGATTTGGA GAAGCCTCCA TGCTAGCCAA 
CAACTACCCT CCACGGAGTT TCAAAATCCT GCTAAGAAAT CCATGAGCTT TGGCGACAAT 
CAGTTGGAGC AAATGGTGGA GCAGGGTCAG CTAGATTTCC CACAGGATTT ACGACGAATT 
TGCCCGAAGC GCGAGACTAT ATACGAGACC TGCTACGATC CCGCCTTCCT GTTACCCCTG 
ATGATGCACT GCTTTGCACC CGAGTCCGTA GTGTGGGCCC GTTGGCCTGT CCAGAATGGA 
TTACTGGCGA TCACCTTCTG CGGTCTATCA TCTCTGGACA AGGACATGAG ACTGGCGGCT 
GCCAGTTGCC AGCAACGCTA TCGTGCTCAT TTCGAGCTCT CCAATTTCTA CGAGCGTCCG 
CTGTGGACGC AGGCCTATGA GAACATTCAG GCAGGATTGG CTGATCTGAG AAACTCCTGG 
CTGGCACAGA GGAGAACGCA TGGCGGAACG CCTAGAGTTC CTTTGATTCC TGGCCTGTTT 
ATCGCTCACA GCTTTAATTT GAGCACAGAG CCAACGCATT TGCTGTACAA GCGACTGATG 
ATGTATTTGC GGCTGAAACA GAGCTTCAAC TTTCAAACGA TACCCGACTT CAATGTTTTC 
TTCTACTCCC AGGAACCAGA GCACCAGCAG TACCGCGAGT TTATCGTGGA GTTGCTTAGA 
TGCGGCATTA AGTGCAGCGC CGACCTTTTC CTGCTGGTAT CCACCAATAC GTTCAAGGTC 
CTCCTTGGAT TCTACGGCTC CAGTTTGGCC AGTTTGGAAA CGAATCTGCT GCTGCTTTCG 
GTGCTCTCCA CATGCGTCAA AATTCCCGGA GCTGTGAAAA TCATGCTGGA GCACGTAGGC 
ATCCTGTCCT GGCTGGTGGG CGTAATCCGC GACACAGAGT TCCATCAGTT CGATATCATC 
GAGGGTGTCA TCAGCATCCT CAACAATCTG TGGTATGCCT TGGCCGCGTC CCGTTCGGAA 
CTGACGGATT ACGACCACAT CCAGTTGCGA GTTTACCGTC TGGTGTTGCA GCTACTGCCG 
CTGCTCTCTC CACGGATATC AGTTCCTGGC TTGGGTCGCC TGTTCAACGT TCTGTGGAAA 
ACTGGCTCGG CCTGTGGCCA GCATCGTGCC GTGTCCGCCA CCCAAATGGA TACCCTGCTG 
GAGTGCGTTT CACGCCAATG GCCCGATCAG CTGGCGAGTG TGCGTTATGT ACGGAGCTTT 
GGCAGCGCTG GCGCCTGTTC GCGTTCCGAA TACTGCGACT GGCTGGCCAA GGATCAAAAG 
CTGGAGACTC CTGCCGTCCT CGCGTTGTCC AGTCTGCGGG AATATGTCAT TGACTGGGCG 
CAAGCGCATA AATCGGAGAG TGACGGGGAA ACCAAGCTGC TGGTGGAACC AACGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG12499 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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