Sar1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sar1
Entrez Gene ID	42615
Full Name	Secretion-associated Ras-related 1
Synonyms	5712,CG7073,Dm Sar1,Dmel\CG7073,NP_732719,Q95SY7,SAR1,anon-EST:Liang-1.48,bro5,clone 1.48,dar3,dsar1,l(3)05712,sar1
General protein information	Preferred Names Secretion-associated Ras-related 1 Names CG7073 gene product from transcript CG7073-RC CG7073-PA CG7073-PC CG7073-PD CG7073-PE Sar1 ortholog Sar1-PA Sar1-PC Sar1-PD Sar1-PE dendritic arbor reduction 3 sar1 secretion-associated RAS-related 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 94A5-94A6

mRNA and Protein(s)

mRNA	Protein	Name
NM_170001.3	NP_732718.1	Secretion-associated Ras-related 1, isoform C
NM_170000.3	NP_732717.1	Secretion-associated Ras-related 1, isoform A
NM_170002.3	NP_732719.1	Secretion-associated Ras-related 1, isoform D
NM_206542.2	NP_996265.1	Secretion-associated Ras-related 1, isoform E

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sar1b	NP_001009622.1
Drosophila melanogaster (fruit fly)	sar1	NP_732718.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004098	XP_003436713.1
Caenorhabditis elegans (roundworm)	sar-1	NP_500582.1
Xenopus tropicalis (tropical clawed frog)	sar1b	NP_001015871.1
Bos taurus (cattle)	SAR1B	NP_001030392.1
Danio rerio (zebrafish)	sar1b	NP_001019548.1
Arabidopsis thaliana (thale cress)	SAR2	NP_192117.1
Pan troglodytes (chimpanzee)	SAR1B	XP_001167618.1
	SAR1B	XP_001109899.1
Homo sapiens (human)	SAR1B	NP_057187.1
Canis lupus familiaris (dog)	SAR1B	XP_538630.2
Oryza sativa (rice)	Os01g0254000	NP_001042615.1
Mus musculus (house mouse)	Sar1b	NP_079811.1
Gallus gallus (chicken)	SAR1B	NP_001025792.1
Arabidopsis thaliana (thale cress)	AT3G62560	NP_191815.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Kruppel-like factor Dar1 promotes dendrite growth in part by suppressing the expression of microtubule-severing protein Spastin.
Title: Differential regulation of dendritic and axonal development by the novel Kr?ppel-like factor Dar1.

dSec16 binds ER cups via its arginine-rich domain, interacts with Sar1-GTP that is generated on ER membrane by Sec12 and concentrates it in the ER cups where it initiates the formation of COPII vesicles
Title: Drosophila Sec16 mediates the biogenesis of tER sites upstream of Sar1 through an arginine-rich motif.

The following Sar1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sar1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05675	NM_170002.3 Latest version!	Drosophila melanogaster Secretion-associated Ras-related 1 (Sar1), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa05675	NM_170001.3 Latest version!	Drosophila melanogaster Secretion-associated Ras-related 1 (Sar1), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa05675	NM_170000.3 Latest version!	Drosophila melanogaster Secretion-associated Ras-related 1 (Sar1), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa05675	NM_206542.2 Latest version!	Drosophila melanogaster Secretion-associated Ras-related 1 (Sar1), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sar1 ( NM_170001.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732718.1 Drosophila melanogaster Secretion-associated Ras-related 1 (Sar1), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa05675
Clone ID Related Accession (Same CDS sequence)	NM_170001.3 , NM_170000.3 , NM_170002.3 , NM_206542.2
Accession Version	NM_170001.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Secretion-associated Ras-related 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170001.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCATCT GGGACTGGTT CACCGGAGTG CTGGGATACC TGGGTCTGTG GAAAAAGTCT 
GGCAAATTAT TGTTCCTGGG CCTGGATAAT GCTGGCAAAA CCACACTCTT GCATATGCTC 
AAAGATGATA AGCTGGCGCA GCATGTGCCC ACACTGCATC CAACATCCGA GGAGCTGTCC 
ATCGGCAACA TGCGCTTCAC TACATTCGAC TTGGGTGGCC ACACTCAGGC ACGACGCGTC 
TGGAAGGACT ACTTCCCTGC TGTGGACGCC ATCGTTTTCT TAATAGACGC CTGGGACCGT 
GGCCGCTTCC AGGAGAGCAA AAACGAGCTG GATTCGCTGC TCACGGATGA GGCGCTGTCC 
AACTGCCCCG TGCTCATATT GGGCAACAAA ATCGATAAGC CCGGCGCGGC TAGCGAGGAT 
GAGCTGAGAA ACGTGTTCGG ACTGTATCAG CTAACAACCG GCAAGGGCAA AGTTGCACGC 
GCCGATTTGC CCGGCCGTCC TCTGGAATTG TTCATGTGCT CCGTGCTGAA GCGACAGGGC 
TACGGCGAGG GTTTCCGTTG GCTGGCGCAG TATATCGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732718.1
CDS	462..1043
Translation	MFIWDWFTGVLGYLGLWKKSGKLLFLGLDNAGKTTLLHMLKDDKLAQHVPTLHPTSEELSIGNMRFTTFDLGGHTQARRVWKDYFPAVDAIVFLIDAWDRGRFQESKNELDSLLTDEALSNCPVLILGNKIDKPGAASEDELRNVFGLYQLTTGKGKVARADLPGRPLELFMCSVLKRQGYGEGFRWLAQYID

Target ORF information:

RefSeq Version	NM_170001.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Secretion-associated Ras-related 1 (Sar1), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170001.3

ORF Insert Sequence:

ATGTTCATCT GGGACTGGTT CACCGGAGTG CTGGGATACC TGGGTCTGTG GAAAAAGTCT 
GGCAAATTAT TGTTCCTGGG CCTGGATAAT GCTGGCAAAA CCACACTCTT GCATATGCTC 
AAAGATGATA AGCTGGCGCA GCATGTGCCC ACACTGCATC CAACATCCGA GGAGCTGTCC 
ATCGGCAACA TGCGCTTCAC TACATTCGAC TTGGGTGGCC ACACTCAGGC ACGACGCGTC 
TGGAAGGACT ACTTCCCTGC TGTGGACGCC ATCGTTTTCT TAATAGACGC CTGGGACCGT 
GGCCGCTTCC AGGAGAGCAA AAACGAGCTG GATTCGCTGC TCACGGATGA GGCGCTGTCC 
AACTGCCCCG TGCTCATATT GGGCAACAAA ATCGATAAGC CCGGCGCGGC TAGCGAGGAT 
GAGCTGAGAA ACGTGTTCGG ACTGTATCAG CTAACAACCG GCAAGGGCAA AGTTGCACGC 
GCCGATTTGC CCGGCCGTCC TCTGGAATTG TTCATGTGCT CCGTGCTGAA GCGACAGGGC 
TACGGCGAGG GTTTCCGTTG GCTGGCGCAG TATATCGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sar1 ( NM_170000.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732717.1 Drosophila melanogaster Secretion-associated Ras-related 1 (Sar1), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa05675
Clone ID Related Accession (Same CDS sequence)	NM_170001.3 , NM_170000.3 , NM_170002.3 , NM_206542.2
Accession Version	NM_170000.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Secretion-associated Ras-related 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170000.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCATCT GGGACTGGTT CACCGGAGTG CTGGGATACC TGGGTCTGTG GAAAAAGTCT 
GGCAAATTAT TGTTCCTGGG CCTGGATAAT GCTGGCAAAA CCACACTCTT GCATATGCTC 
AAAGATGATA AGCTGGCGCA GCATGTGCCC ACACTGCATC CAACATCCGA GGAGCTGTCC 
ATCGGCAACA TGCGCTTCAC TACATTCGAC TTGGGTGGCC ACACTCAGGC ACGACGCGTC 
TGGAAGGACT ACTTCCCTGC TGTGGACGCC ATCGTTTTCT TAATAGACGC CTGGGACCGT 
GGCCGCTTCC AGGAGAGCAA AAACGAGCTG GATTCGCTGC TCACGGATGA GGCGCTGTCC 
AACTGCCCCG TGCTCATATT GGGCAACAAA ATCGATAAGC CCGGCGCGGC TAGCGAGGAT 
GAGCTGAGAA ACGTGTTCGG ACTGTATCAG CTAACAACCG GCAAGGGCAA AGTTGCACGC 
GCCGATTTGC CCGGCCGTCC TCTGGAATTG TTCATGTGCT CCGTGCTGAA GCGACAGGGC 
TACGGCGAGG GTTTCCGTTG GCTGGCGCAG TATATCGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732717.1
CDS	164..745
Translation	MFIWDWFTGVLGYLGLWKKSGKLLFLGLDNAGKTTLLHMLKDDKLAQHVPTLHPTSEELSIGNMRFTTFDLGGHTQARRVWKDYFPAVDAIVFLIDAWDRGRFQESKNELDSLLTDEALSNCPVLILGNKIDKPGAASEDELRNVFGLYQLTTGKGKVARADLPGRPLELFMCSVLKRQGYGEGFRWLAQYID

Target ORF information:

RefSeq Version	NM_170000.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Secretion-associated Ras-related 1 (Sar1), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170000.3

ORF Insert Sequence:

ATGTTCATCT GGGACTGGTT CACCGGAGTG CTGGGATACC TGGGTCTGTG GAAAAAGTCT 
GGCAAATTAT TGTTCCTGGG CCTGGATAAT GCTGGCAAAA CCACACTCTT GCATATGCTC 
AAAGATGATA AGCTGGCGCA GCATGTGCCC ACACTGCATC CAACATCCGA GGAGCTGTCC 
ATCGGCAACA TGCGCTTCAC TACATTCGAC TTGGGTGGCC ACACTCAGGC ACGACGCGTC 
TGGAAGGACT ACTTCCCTGC TGTGGACGCC ATCGTTTTCT TAATAGACGC CTGGGACCGT 
GGCCGCTTCC AGGAGAGCAA AAACGAGCTG GATTCGCTGC TCACGGATGA GGCGCTGTCC 
AACTGCCCCG TGCTCATATT GGGCAACAAA ATCGATAAGC CCGGCGCGGC TAGCGAGGAT 
GAGCTGAGAA ACGTGTTCGG ACTGTATCAG CTAACAACCG GCAAGGGCAA AGTTGCACGC 
GCCGATTTGC CCGGCCGTCC TCTGGAATTG TTCATGTGCT CCGTGCTGAA GCGACAGGGC 
TACGGCGAGG GTTTCCGTTG GCTGGCGCAG TATATCGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sar1 ( NM_170002.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732719.1 Drosophila melanogaster Secretion-associated Ras-related 1 (Sar1), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa05675
Clone ID Related Accession (Same CDS sequence)	NM_170001.3 , NM_170000.3 , NM_170002.3 , NM_206542.2
Accession Version	NM_170002.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Secretion-associated Ras-related 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_170002.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCATCT GGGACTGGTT CACCGGAGTG CTGGGATACC TGGGTCTGTG GAAAAAGTCT 
GGCAAATTAT TGTTCCTGGG CCTGGATAAT GCTGGCAAAA CCACACTCTT GCATATGCTC 
AAAGATGATA AGCTGGCGCA GCATGTGCCC ACACTGCATC CAACATCCGA GGAGCTGTCC 
ATCGGCAACA TGCGCTTCAC TACATTCGAC TTGGGTGGCC ACACTCAGGC ACGACGCGTC 
TGGAAGGACT ACTTCCCTGC TGTGGACGCC ATCGTTTTCT TAATAGACGC CTGGGACCGT 
GGCCGCTTCC AGGAGAGCAA AAACGAGCTG GATTCGCTGC TCACGGATGA GGCGCTGTCC 
AACTGCCCCG TGCTCATATT GGGCAACAAA ATCGATAAGC CCGGCGCGGC TAGCGAGGAT 
GAGCTGAGAA ACGTGTTCGG ACTGTATCAG CTAACAACCG GCAAGGGCAA AGTTGCACGC 
GCCGATTTGC CCGGCCGTCC TCTGGAATTG TTCATGTGCT CCGTGCTGAA GCGACAGGGC 
TACGGCGAGG GTTTCCGTTG GCTGGCGCAG TATATCGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732719.1
CDS	213..794
Translation	MFIWDWFTGVLGYLGLWKKSGKLLFLGLDNAGKTTLLHMLKDDKLAQHVPTLHPTSEELSIGNMRFTTFDLGGHTQARRVWKDYFPAVDAIVFLIDAWDRGRFQESKNELDSLLTDEALSNCPVLILGNKIDKPGAASEDELRNVFGLYQLTTGKGKVARADLPGRPLELFMCSVLKRQGYGEGFRWLAQYID

Target ORF information:

RefSeq Version	NM_170002.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Secretion-associated Ras-related 1 (Sar1), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170002.3

ORF Insert Sequence:

ATGTTCATCT GGGACTGGTT CACCGGAGTG CTGGGATACC TGGGTCTGTG GAAAAAGTCT 
GGCAAATTAT TGTTCCTGGG CCTGGATAAT GCTGGCAAAA CCACACTCTT GCATATGCTC 
AAAGATGATA AGCTGGCGCA GCATGTGCCC ACACTGCATC CAACATCCGA GGAGCTGTCC 
ATCGGCAACA TGCGCTTCAC TACATTCGAC TTGGGTGGCC ACACTCAGGC ACGACGCGTC 
TGGAAGGACT ACTTCCCTGC TGTGGACGCC ATCGTTTTCT TAATAGACGC CTGGGACCGT 
GGCCGCTTCC AGGAGAGCAA AAACGAGCTG GATTCGCTGC TCACGGATGA GGCGCTGTCC 
AACTGCCCCG TGCTCATATT GGGCAACAAA ATCGATAAGC CCGGCGCGGC TAGCGAGGAT 
GAGCTGAGAA ACGTGTTCGG ACTGTATCAG CTAACAACCG GCAAGGGCAA AGTTGCACGC 
GCCGATTTGC CCGGCCGTCC TCTGGAATTG TTCATGTGCT CCGTGCTGAA GCGACAGGGC 
TACGGCGAGG GTTTCCGTTG GCTGGCGCAG TATATCGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sar1 ( NM_206542.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996265.1 Drosophila melanogaster Secretion-associated Ras-related 1 (Sar1), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa05675
Clone ID Related Accession (Same CDS sequence)	NM_170001.3 , NM_170000.3 , NM_170002.3 , NM_206542.2
Accession Version	NM_206542.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Secretion-associated Ras-related 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_206542.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCATCT GGGACTGGTT CACCGGAGTG CTGGGATACC TGGGTCTGTG GAAAAAGTCT 
GGCAAATTAT TGTTCCTGGG CCTGGATAAT GCTGGCAAAA CCACACTCTT GCATATGCTC 
AAAGATGATA AGCTGGCGCA GCATGTGCCC ACACTGCATC CAACATCCGA GGAGCTGTCC 
ATCGGCAACA TGCGCTTCAC TACATTCGAC TTGGGTGGCC ACACTCAGGC ACGACGCGTC 
TGGAAGGACT ACTTCCCTGC TGTGGACGCC ATCGTTTTCT TAATAGACGC CTGGGACCGT 
GGCCGCTTCC AGGAGAGCAA AAACGAGCTG GATTCGCTGC TCACGGATGA GGCGCTGTCC 
AACTGCCCCG TGCTCATATT GGGCAACAAA ATCGATAAGC CCGGCGCGGC TAGCGAGGAT 
GAGCTGAGAA ACGTGTTCGG ACTGTATCAG CTAACAACCG GCAAGGGCAA AGTTGCACGC 
GCCGATTTGC CCGGCCGTCC TCTGGAATTG TTCATGTGCT CCGTGCTGAA GCGACAGGGC 
TACGGCGAGG GTTTCCGTTG GCTGGCGCAG TATATCGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996265.1
CDS	113..694
Translation	MFIWDWFTGVLGYLGLWKKSGKLLFLGLDNAGKTTLLHMLKDDKLAQHVPTLHPTSEELSIGNMRFTTFDLGGHTQARRVWKDYFPAVDAIVFLIDAWDRGRFQESKNELDSLLTDEALSNCPVLILGNKIDKPGAASEDELRNVFGLYQLTTGKGKVARADLPGRPLELFMCSVLKRQGYGEGFRWLAQYID

Target ORF information:

RefSeq Version	NM_206542.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Secretion-associated Ras-related 1 (Sar1), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206542.2

ORF Insert Sequence:

ATGTTCATCT GGGACTGGTT CACCGGAGTG CTGGGATACC TGGGTCTGTG GAAAAAGTCT 
GGCAAATTAT TGTTCCTGGG CCTGGATAAT GCTGGCAAAA CCACACTCTT GCATATGCTC 
AAAGATGATA AGCTGGCGCA GCATGTGCCC ACACTGCATC CAACATCCGA GGAGCTGTCC 
ATCGGCAACA TGCGCTTCAC TACATTCGAC TTGGGTGGCC ACACTCAGGC ACGACGCGTC 
TGGAAGGACT ACTTCCCTGC TGTGGACGCC ATCGTTTTCT TAATAGACGC CTGGGACCGT 
GGCCGCTTCC AGGAGAGCAA AAACGAGCTG GATTCGCTGC TCACGGATGA GGCGCTGTCC 
AACTGCCCCG TGCTCATATT GGGCAACAAA ATCGATAAGC CCGGCGCGGC TAGCGAGGAT 
GAGCTGAGAA ACGTGTTCGG ACTGTATCAG CTAACAACCG GCAAGGGCAA AGTTGCACGC 
GCCGATTTGC CCGGCCGTCC TCTGGAATTG TTCATGTGCT CCGTGCTGAA GCGACAGGGC 
TACGGCGAGG GTTTCCGTTG GCTGGCGCAG TATATCGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Sar1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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