how cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	how
Entrez Gene ID	42596
Full Name	held out wings
Synonyms	0904/17,24B,CG10293,Dmel\CG10293,HOW,How,KH93F,P62,SZ1,Who/How,anon-EST:Liang-2.39,clone 2.39,l(3)S053606,l(3)S090417,l(3)j5B5,l(3)j5D5,l(3)s2612,qkr,qkr[93F],sci,stru,who
General protein information	Preferred Names held out wings Names CG10293 gene product from transcript CG10293-RF CG10293-PA CG10293-PB CG10293-PC CG10293-PD CG10293-PE CG10293-PF Held-out wings KH motif at 93F held Out wing how-PA how-PB how-PC how-PD how-PE how-PF lethal (3) S053606 lethal (3) S090417 quaking related, 93F scorpion struthio wings held out wings held-out
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 87F3-87F10

mRNA and Protein(s)

mRNA	Protein	Name
NM_001170212.2	NP_001163683.1	held out wings, isoform D
NM_169992.3	NP_732695.1	held out wings, isoform B
NM_001170213.2	NP_001163684.1	held out wings, isoform E
NM_079723.4	NP_524447.2	held out wings, isoform A
NM_001032032.3	NP_001027203.1	held out wings, isoform C
NM_001275893.1	NP_001262822.1	held out wings, isoform F

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	qkia	NP_571299.1
Drosophila melanogaster (fruit fly)	how	NP_001163683.1
Danio rerio (zebrafish)	LOC100537631	XP_005158869.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Identification of two putative HOW Response Elements within the dgrasp open reading frame for binding to Held Out Wings (HOW).
Title: The Drosophila RNA-binding protein HOW controls the stability of dgrasp mRNA in the follicular epithelium.

HOW is required for SXL to retain msl2 transcripts in the nucleus
Title: Sex-lethal promotes nuclear retention of msl2 mRNA via interactions with the STAR protein HOW.

The regulation of glial-specific splicing of Neurexin IV requires HOW and Cdk12 activity.
Title: The regulation of glial-specific splicing of Neurexin IV requires HOW and Cdk12 activity.

MAPK/ERK-dependent phosphorylation of HOW promotes the formation of HOW dimers and thus enhances its activity in controlling mRNA levels of key muscle-specific genes
Title: Phosphorylation of the Drosophila melanogaster RNA-binding protein HOW by MAPK/ERK enhances its dimerization and activity.

HOW has different roles in male and female Drosophila germline differentiation
Title: Differential roles of HOW in male and female Drosophila germline differentiation.

The following how gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the how cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05655	NM_079723.4 Latest version!	Drosophila melanogaster held out wings (how), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa05655	NM_001170212.2 Latest version!	Drosophila melanogaster held out wings (how), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa05656	NM_001032032.3 Latest version!	Drosophila melanogaster held out wings (how), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa05657	NM_169992.3 Latest version!	Drosophila melanogaster held out wings (how), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa08634	NM_001275893.1 Latest version!	Drosophila melanogaster held out wings (how), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa08635	NM_001170213.2 Latest version!	Drosophila melanogaster held out wings (how), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

how ( NM_001170212.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163683.1 Drosophila melanogaster held out wings (how), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa05655
Clone ID Related Accession (Same CDS sequence)	NM_001170212.2 , NM_079723.4
Accession Version	NM_001170212.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	held out wings, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170212.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGTCT GTGAGAGCAA AGCCGTTGTG CAACAGCAAC TGCAGCAGCA CTTGCAGCAA 
CAGGCAGCCG CAGCAGTTGT TGCGGTCGCG CAACAGCAGC AGGCTCAAGC CCAAGCCCAG 
GCTCAGGCTC AGGCACAGCA GCAGCAACAG GCGCCGCAGG TGGTGGTCCC CATGACCCCG 
CAGCACTTGA CCCCACAGCA GCAGCAGCAG AGCACACAGA GCATCGCCGA CTATCTGGCC 
CAGTTGCTCA AGGACCGCAA GCAACTGGCC GCCTTCCCCA ACGTCTTCAC CCACGTCGAA 
CGCCTGCTGG ACGAAGAAAT TGCACGCGTG CGCGCCTCAC TGTTCCAGAT CAATGGGGTC 
AAGAAGGAGC CGCTCACTCT GCCCGAACCC GAGGGCTCTG TGGTGACGAT GAACGAGAAG 
GTTTATGTGC CAGTCCGCGA GCATCCAGAT TTCAACTTTG TCGGTCGCAT TTTGGGACCC 
CGTGGCATGA CCGCCAAGCA ATTGGAACAG GAGACCGGCT GCAAGATTAT GGTCCGAGGC 
AAGGGTTCCA TGCGCGACAA GAAGAAGGAG GACGCCAACC GTGGCAAGCC TAACTGGGAG 
CATCTGTCCG ATGACCTGCA TGTCCTGATA ACCGTCGAGG ACACCGAGAA CCGTGCCACA 
GTGAAGTTGG CCCAGGCCGT CGCCGAAGTA CAGAAGTTGC TCGTGCCGCA AGCCGAAGGC 
GAAGATGAGC TAAAGAAACG TCAACTCATG GAATTGGCGA TTATTAATGG CACTTATAGG 
GACACAACAG CGAAATCTGT CGCAGTGTGC GATGAGGAGT GGCGCCGCCT GGTTGCCGCC 
TCTGATAGCC GCCTGCTGAC ATCCACCGGC CTGCCCGGCC TTGCCGCCCA GATCCGTGCA 
CCCGCCGCCG CCCCGCTTGG CGCCCCATTG ATCCTGAATC CCCGGATGAC CGTCCCCACA 
ACGGCGGCCA GCATATTGTC CGCCCAGGCC GCTCCGACAG CCGCCTTCGA CCAGACCGGC 
CATGGAATGA TCTTCGCACC GTACGATTAT GCGAACTACG CCGCCCTAGC CGGCAATCCT 
CTGCTCACGG AATATGCTGA TCATAGCGTA GGCGCCATTA AGCAGCAGCG TCGTTTGGCG 
ACTAACCGCG AGCATCCCTA TCAGCGAGCA ACGGTCGGAG TGCCAGCCAA GCCGGCGGGT 
TTCATTGAGA TACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163683.1
CDS	323..1540
Translation	MSVCESKAVVQQQLQQHLQQQAAAAVVAVAQQQQAQAQAQAQAQAQQQQQAPQVVVPMTPQHLTPQQQQQSTQSIADYLAQLLKDRKQLAAFPNVFTHVERLLDEEIARVRASLFQINGVKKEPLTLPEPEGSVVTMNEKVYVPVREHPDFNFVGRILGPRGMTAKQLEQETGCKIMVRGKGSMRDKKKEDANRGKPNWEHLSDDLHVLITVEDTENRATVKLAQAVAEVQKLLVPQAEGEDELKKRQLMELAIINGTYRDTTAKSVAVCDEEWRRLVAASDSRLLTSTGLPGLAAQIRAPAAAPLGAPLILNPRMTVPTTAASILSAQAAPTAAFDQTGHGMIFAPYDYANYAALAGNPLLTEYADHSVGAIKQQRRLATNREHPYQRATVGVPAKPAGFIEIQ

Target ORF information:

RefSeq Version	NM_001170212.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster held out wings (how), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170212.2

ORF Insert Sequence:

ATGAGTGTCT GTGAGAGCAA AGCCGTTGTG CAACAGCAAC TGCAGCAGCA CTTGCAGCAA 
CAGGCAGCCG CAGCAGTTGT TGCGGTCGCG CAACAGCAGC AGGCTCAAGC CCAAGCCCAG 
GCTCAGGCTC AGGCACAGCA GCAGCAACAG GCGCCGCAGG TGGTGGTCCC CATGACCCCG 
CAGCACTTGA CCCCACAGCA GCAGCAGCAG AGCACACAGA GCATCGCCGA CTATCTGGCC 
CAGTTGCTCA AGGACCGCAA GCAACTGGCC GCCTTCCCCA ACGTCTTCAC CCACGTCGAA 
CGCCTGCTGG ACGAAGAAAT TGCACGCGTG CGCGCCTCAC TGTTCCAGAT CAATGGGGTC 
AAGAAGGAGC CGCTCACTCT GCCCGAACCC GAGGGCTCTG TGGTGACGAT GAACGAGAAG 
GTTTATGTGC CAGTCCGCGA GCATCCAGAT TTCAACTTTG TCGGTCGCAT TTTGGGACCC 
CGTGGCATGA CCGCCAAGCA ATTGGAACAG GAGACCGGCT GCAAGATTAT GGTCCGAGGC 
AAGGGTTCCA TGCGCGACAA GAAGAAGGAG GACGCCAACC GTGGCAAGCC TAACTGGGAG 
CATCTGTCCG ATGACCTGCA TGTCCTGATA ACCGTCGAGG ACACCGAGAA CCGTGCCACA 
GTGAAGTTGG CCCAGGCCGT CGCCGAAGTA CAGAAGTTGC TCGTGCCGCA AGCCGAAGGC 
GAAGATGAGC TAAAGAAACG TCAACTCATG GAATTGGCGA TTATTAATGG CACTTATAGG 
GACACAACAG CGAAATCTGT CGCAGTGTGC GATGAGGAGT GGCGCCGCCT GGTTGCCGCC 
TCTGATAGCC GCCTGCTGAC ATCCACCGGC CTGCCCGGCC TTGCCGCCCA GATCCGTGCA 
CCCGCCGCCG CCCCGCTTGG CGCCCCATTG ATCCTGAATC CCCGGATGAC CGTCCCCACA 
ACGGCGGCCA GCATATTGTC CGCCCAGGCC GCTCCGACAG CCGCCTTCGA CCAGACCGGC 
CATGGAATGA TCTTCGCACC GTACGATTAT GCGAACTACG CCGCCCTAGC CGGCAATCCT 
CTGCTCACGG AATATGCTGA TCATAGCGTA GGCGCCATTA AGCAGCAGCG TCGTTTGGCG 
ACTAACCGCG AGCATCCCTA TCAGCGAGCA ACGGTCGGAG TGCCAGCCAA GCCGGCGGGT 
TTCATTGAGA TACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

how ( NM_079723.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524447.2 Drosophila melanogaster held out wings (how), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa05655
Clone ID Related Accession (Same CDS sequence)	NM_001170212.2 , NM_079723.4
Accession Version	NM_079723.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	held out wings, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079723.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGTCT GTGAGAGCAA AGCCGTTGTG CAACAGCAAC TGCAGCAGCA CTTGCAGCAA 
CAGGCAGCCG CAGCAGTTGT TGCGGTCGCG CAACAGCAGC AGGCTCAAGC CCAAGCCCAG 
GCTCAGGCTC AGGCACAGCA GCAGCAACAG GCGCCGCAGG TGGTGGTCCC CATGACCCCG 
CAGCACTTGA CCCCACAGCA GCAGCAGCAG AGCACACAGA GCATCGCCGA CTATCTGGCC 
CAGTTGCTCA AGGACCGCAA GCAACTGGCC GCCTTCCCCA ACGTCTTCAC CCACGTCGAA 
CGCCTGCTGG ACGAAGAAAT TGCACGCGTG CGCGCCTCAC TGTTCCAGAT CAATGGGGTC 
AAGAAGGAGC CGCTCACTCT GCCCGAACCC GAGGGCTCTG TGGTGACGAT GAACGAGAAG 
GTTTATGTGC CAGTCCGCGA GCATCCAGAT TTCAACTTTG TCGGTCGCAT TTTGGGACCC 
CGTGGCATGA CCGCCAAGCA ATTGGAACAG GAGACCGGCT GCAAGATTAT GGTCCGAGGC 
AAGGGTTCCA TGCGCGACAA GAAGAAGGAG GACGCCAACC GTGGCAAGCC TAACTGGGAG 
CATCTGTCCG ATGACCTGCA TGTCCTGATA ACCGTCGAGG ACACCGAGAA CCGTGCCACA 
GTGAAGTTGG CCCAGGCCGT CGCCGAAGTA CAGAAGTTGC TCGTGCCGCA AGCCGAAGGC 
GAAGATGAGC TAAAGAAACG TCAACTCATG GAATTGGCGA TTATTAATGG CACTTATAGG 
GACACAACAG CGAAATCTGT CGCAGTGTGC GATGAGGAGT GGCGCCGCCT GGTTGCCGCC 
TCTGATAGCC GCCTGCTGAC ATCCACCGGC CTGCCCGGCC TTGCCGCCCA GATCCGTGCA 
CCCGCCGCCG CCCCGCTTGG CGCCCCATTG ATCCTGAATC CCCGGATGAC CGTCCCCACA 
ACGGCGGCCA GCATATTGTC CGCCCAGGCC GCTCCGACAG CCGCCTTCGA CCAGACCGGC 
CATGGAATGA TCTTCGCACC GTACGATTAT GCGAACTACG CCGCCCTAGC CGGCAATCCT 
CTGCTCACGG AATATGCTGA TCATAGCGTA GGCGCCATTA AGCAGCAGCG TCGTTTGGCG 
ACTAACCGCG AGCATCCCTA TCAGCGAGCA ACGGTCGGAG TGCCAGCCAA GCCGGCGGGT 
TTCATTGAGA TACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524447.2
CDS	323..1540
Translation	MSVCESKAVVQQQLQQHLQQQAAAAVVAVAQQQQAQAQAQAQAQAQQQQQAPQVVVPMTPQHLTPQQQQQSTQSIADYLAQLLKDRKQLAAFPNVFTHVERLLDEEIARVRASLFQINGVKKEPLTLPEPEGSVVTMNEKVYVPVREHPDFNFVGRILGPRGMTAKQLEQETGCKIMVRGKGSMRDKKKEDANRGKPNWEHLSDDLHVLITVEDTENRATVKLAQAVAEVQKLLVPQAEGEDELKKRQLMELAIINGTYRDTTAKSVAVCDEEWRRLVAASDSRLLTSTGLPGLAAQIRAPAAAPLGAPLILNPRMTVPTTAASILSAQAAPTAAFDQTGHGMIFAPYDYANYAALAGNPLLTEYADHSVGAIKQQRRLATNREHPYQRATVGVPAKPAGFIEIQ

Target ORF information:

RefSeq Version	NM_079723.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster held out wings (how), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079723.4

ORF Insert Sequence:

ATGAGTGTCT GTGAGAGCAA AGCCGTTGTG CAACAGCAAC TGCAGCAGCA CTTGCAGCAA 
CAGGCAGCCG CAGCAGTTGT TGCGGTCGCG CAACAGCAGC AGGCTCAAGC CCAAGCCCAG 
GCTCAGGCTC AGGCACAGCA GCAGCAACAG GCGCCGCAGG TGGTGGTCCC CATGACCCCG 
CAGCACTTGA CCCCACAGCA GCAGCAGCAG AGCACACAGA GCATCGCCGA CTATCTGGCC 
CAGTTGCTCA AGGACCGCAA GCAACTGGCC GCCTTCCCCA ACGTCTTCAC CCACGTCGAA 
CGCCTGCTGG ACGAAGAAAT TGCACGCGTG CGCGCCTCAC TGTTCCAGAT CAATGGGGTC 
AAGAAGGAGC CGCTCACTCT GCCCGAACCC GAGGGCTCTG TGGTGACGAT GAACGAGAAG 
GTTTATGTGC CAGTCCGCGA GCATCCAGAT TTCAACTTTG TCGGTCGCAT TTTGGGACCC 
CGTGGCATGA CCGCCAAGCA ATTGGAACAG GAGACCGGCT GCAAGATTAT GGTCCGAGGC 
AAGGGTTCCA TGCGCGACAA GAAGAAGGAG GACGCCAACC GTGGCAAGCC TAACTGGGAG 
CATCTGTCCG ATGACCTGCA TGTCCTGATA ACCGTCGAGG ACACCGAGAA CCGTGCCACA 
GTGAAGTTGG CCCAGGCCGT CGCCGAAGTA CAGAAGTTGC TCGTGCCGCA AGCCGAAGGC 
GAAGATGAGC TAAAGAAACG TCAACTCATG GAATTGGCGA TTATTAATGG CACTTATAGG 
GACACAACAG CGAAATCTGT CGCAGTGTGC GATGAGGAGT GGCGCCGCCT GGTTGCCGCC 
TCTGATAGCC GCCTGCTGAC ATCCACCGGC CTGCCCGGCC TTGCCGCCCA GATCCGTGCA 
CCCGCCGCCG CCCCGCTTGG CGCCCCATTG ATCCTGAATC CCCGGATGAC CGTCCCCACA 
ACGGCGGCCA GCATATTGTC CGCCCAGGCC GCTCCGACAG CCGCCTTCGA CCAGACCGGC 
CATGGAATGA TCTTCGCACC GTACGATTAT GCGAACTACG CCGCCCTAGC CGGCAATCCT 
CTGCTCACGG AATATGCTGA TCATAGCGTA GGCGCCATTA AGCAGCAGCG TCGTTTGGCG 
ACTAACCGCG AGCATCCCTA TCAGCGAGCA ACGGTCGGAG TGCCAGCCAA GCCGGCGGGT 
TTCATTGAGA TACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

how ( NM_001032032.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001027203.1 Drosophila melanogaster held out wings (how), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa05656
Clone ID Related Accession (Same CDS sequence)	NM_001032032.3
Accession Version	NM_001032032.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	held out wings, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001032032.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGTCT GTGAGAGCAA AGCCGTTGTG CAACAGCAAC TGCAGCAGCA CTTGCAGCAA 
CAGGCAGCCG CAGCAGTTGT TGCGGTCGCG CAACAGCAGC AGGCTCAAGC CCAAGCCCAG 
GCTCAGGCTC AGGCACAGCA GCAGCAACAG GCGCCGCAGG TGGTGGTCCC CATGACCCCG 
CAGCACTTGA CCCCACAGCA GCAGCAGCAG AGCACACAGA GCATCGCCGA CTATCTGGCC 
CAGTTGCTCA AGGACCGCAA GCAACTGGCC GCCTTCCCCA ACGTCTTCAC CCACGTCGAA 
CGCCTGCTGG ACGAAGAAAT TGCACGCGTG CGCGCCTCAC TGTTCCAGAT CAATGGGGTC 
AAGAAGGAGC CGCTCACTCT GCCCGAACCC GAGGGCTCTG TGGTGACGAT GAACGAGAAG 
GTTTATGTGC CAGTCCGCGA GCATCCAGAT TTCAACTTTG TCGGTCGCAT TTTGGGACCC 
CGTGGCATGA CCGCCAAGCA ATTGGAACAG GAGACCGGCT GCAAGATTAT GGTCCGAGGC 
AAGGGTTCCA TGCGCGACAA GAAGAAGGAG GACGCCAACC GTGGCAAGCC TAACTGGGAG 
CATCTGTCCG ATGACCTGCA TGTCCTGATA ACCGTCGAGG ACACCGAGAA CCGTGCCACA 
GTGAAGTTGG CCCAGGCCGT CGCCGAAGTA CAGAAGTTGC TCGTGCCGCA AGCCGAAGGC 
GAAGATGAGC TAAAGAAACG TCAACTCATG GAATTGGCGA TTATTAATGG CACTTATAGG 
GACACAACAG CGAAATCTGT CGCAGTGTGC GATGAGGAGT GGCGCCGCCT GGTTGCCGCC 
TCTGATAGCC GCCTGCTGAC ATCCACCGGC CTGCCCGGCC TTGCCGCCCA GATCCGTGCA 
CCCGCCGCCG CCCCGCTTGG CGCCCCATTG ATCCTGAATC CCCGGATGAC CGTCCCCACA 
ACGGCGGCCA GCATATTGTC CGCCCAGGCC GCTCCGACAG CCGCCTTCGA CCAGACCGGC 
CATGGAATGA TCTTCGCACC GTACGATTAT GCGAACTACG CCGCCCTAGC CGGCAATCCT 
CTGCTCACGG AATATGCTGA TCATAGCGTT GGTCGATATA TGCATGCCGG CTCCGTCTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027203.1
CDS	323..1465
Translation	MSVCESKAVVQQQLQQHLQQQAAAAVVAVAQQQQAQAQAQAQAQAQQQQQAPQVVVPMTPQHLTPQQQQQSTQSIADYLAQLLKDRKQLAAFPNVFTHVERLLDEEIARVRASLFQINGVKKEPLTLPEPEGSVVTMNEKVYVPVREHPDFNFVGRILGPRGMTAKQLEQETGCKIMVRGKGSMRDKKKEDANRGKPNWEHLSDDLHVLITVEDTENRATVKLAQAVAEVQKLLVPQAEGEDELKKRQLMELAIINGTYRDTTAKSVAVCDEEWRRLVAASDSRLLTSTGLPGLAAQIRAPAAAPLGAPLILNPRMTVPTTAASILSAQAAPTAAFDQTGHGMIFAPYDYANYAALAGNPLLTEYADHSVGRYMHAGSVF

Target ORF information:

RefSeq Version	NM_001032032.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster held out wings (how), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001032032.3

ORF Insert Sequence:

ATGAGTGTCT GTGAGAGCAA AGCCGTTGTG CAACAGCAAC TGCAGCAGCA CTTGCAGCAA 
CAGGCAGCCG CAGCAGTTGT TGCGGTCGCG CAACAGCAGC AGGCTCAAGC CCAAGCCCAG 
GCTCAGGCTC AGGCACAGCA GCAGCAACAG GCGCCGCAGG TGGTGGTCCC CATGACCCCG 
CAGCACTTGA CCCCACAGCA GCAGCAGCAG AGCACACAGA GCATCGCCGA CTATCTGGCC 
CAGTTGCTCA AGGACCGCAA GCAACTGGCC GCCTTCCCCA ACGTCTTCAC CCACGTCGAA 
CGCCTGCTGG ACGAAGAAAT TGCACGCGTG CGCGCCTCAC TGTTCCAGAT CAATGGGGTC 
AAGAAGGAGC CGCTCACTCT GCCCGAACCC GAGGGCTCTG TGGTGACGAT GAACGAGAAG 
GTTTATGTGC CAGTCCGCGA GCATCCAGAT TTCAACTTTG TCGGTCGCAT TTTGGGACCC 
CGTGGCATGA CCGCCAAGCA ATTGGAACAG GAGACCGGCT GCAAGATTAT GGTCCGAGGC 
AAGGGTTCCA TGCGCGACAA GAAGAAGGAG GACGCCAACC GTGGCAAGCC TAACTGGGAG 
CATCTGTCCG ATGACCTGCA TGTCCTGATA ACCGTCGAGG ACACCGAGAA CCGTGCCACA 
GTGAAGTTGG CCCAGGCCGT CGCCGAAGTA CAGAAGTTGC TCGTGCCGCA AGCCGAAGGC 
GAAGATGAGC TAAAGAAACG TCAACTCATG GAATTGGCGA TTATTAATGG CACTTATAGG 
GACACAACAG CGAAATCTGT CGCAGTGTGC GATGAGGAGT GGCGCCGCCT GGTTGCCGCC 
TCTGATAGCC GCCTGCTGAC ATCCACCGGC CTGCCCGGCC TTGCCGCCCA GATCCGTGCA 
CCCGCCGCCG CCCCGCTTGG CGCCCCATTG ATCCTGAATC CCCGGATGAC CGTCCCCACA 
ACGGCGGCCA GCATATTGTC CGCCCAGGCC GCTCCGACAG CCGCCTTCGA CCAGACCGGC 
CATGGAATGA TCTTCGCACC GTACGATTAT GCGAACTACG CCGCCCTAGC CGGCAATCCT 
CTGCTCACGG AATATGCTGA TCATAGCGTT GGTCGATATA TGCATGCCGG CTCCGTCTTT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

how ( NM_169992.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732695.1 Drosophila melanogaster held out wings (how), transcript variant B, mRNA.

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CloneID	OFa05657
Clone ID Related Accession (Same CDS sequence)	NM_169992.3
Accession Version	NM_169992.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	held out wings, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169992.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGTCT GTGAGAGCAA AGCCGTTGTG CAACAGCAAC TGCAGCAGCA CTTGCAGCAA 
CAGGCAGCCG CAGCAGTTGT TGCGGTCGCG CAACAGCAGC AGGCTCAAGC CCAAGCCCAG 
GCTCAGGCTC AGGCACAGCA GCAGCAACAG GCGCCGCAGG TGGTGGTCCC CATGACCCCG 
CAGCACTTGA CCCCACAGCA GCAGCAGCAG AGCACACAGA GCATCGCCGA CTATCTGGCC 
CAGTTGCTCA AGGACCGCAA GCAACTGGCC GCCTTCCCCA ACGTCTTCAC CCACGTCGAA 
CGCCTGCTGG ACGAAGAAAT TGCACGCGTG CGCGCCTCAC TGTTCCAGAT CAATGGGGTC 
AAGAAGGAGC CGCTCACTCT GCCCGAACCC GAGGGCTCTG TGGTGACGAT GAACGAGAAG 
GTTTATGTGC CAGTCCGCGA GCATCCAGAT TTCAACTTTG TCGGTCGCAT TTTGGGACCC 
CGTGGCATGA CCGCCAAGCA ATTGGAACAG GAGACCGGCT GCAAGATTAT GGTCCGAGGC 
AAGGGTTCCA TGCGCGACAA GAAGAAGGAG GACGCCAACC GTGGCAAGCC TAACTGGGAG 
CATCTGTCCG ATGACCTGCA TGTCCTGATA ACCGTCGAGG ACACCGAGAA CCGTGCCACA 
GTGAAGTTGG CCCAGGCCGT CGCCGAAGTA CAGAAGTTGC TCGTGCCGCA AGCCGAAGGC 
GAAGATGAGC TAAAGAAACG TCAACTCATG GAATTGGCGA TTATTAATGG CACTTATAGG 
GACACAACAG CGAAATCTGT CGCAGTGTGC GATGAGGAGT GGCGCCGCCT GGTTGCCGCC 
TCTGATAGCC GCCTGCTGAC ATCCACCGGC CTGCCCGGCC TTGCCGCCCA GATCCGTGCA 
CCCGCCGCCG CCCCGCTTGG CGCCCCATTG ATCCTGAATC CCCGGATGAC CGTCCCCACA 
ACGGCGGCCA GCATATTGTC CGCCCAGGCC GCTCCGACAG CCGCCTTCGA CCAGACCGGC 
CATGGAATGA TCTTCGCACC GTACGATTAT GCGAACTACG CCGCCCTAGC CGGCAATCCT 
CTGCTCACGG AATATGCTGA TCATAGCGGT GGGTTGTTTG CCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732695.1
CDS	323..1450
Translation	MSVCESKAVVQQQLQQHLQQQAAAAVVAVAQQQQAQAQAQAQAQAQQQQQAPQVVVPMTPQHLTPQQQQQSTQSIADYLAQLLKDRKQLAAFPNVFTHVERLLDEEIARVRASLFQINGVKKEPLTLPEPEGSVVTMNEKVYVPVREHPDFNFVGRILGPRGMTAKQLEQETGCKIMVRGKGSMRDKKKEDANRGKPNWEHLSDDLHVLITVEDTENRATVKLAQAVAEVQKLLVPQAEGEDELKKRQLMELAIINGTYRDTTAKSVAVCDEEWRRLVAASDSRLLTSTGLPGLAAQIRAPAAAPLGAPLILNPRMTVPTTAASILSAQAAPTAAFDQTGHGMIFAPYDYANYAALAGNPLLTEYADHSGGLFAR

Target ORF information:

RefSeq Version	NM_169992.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster held out wings (how), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169992.3

ORF Insert Sequence:

ATGAGTGTCT GTGAGAGCAA AGCCGTTGTG CAACAGCAAC TGCAGCAGCA CTTGCAGCAA 
CAGGCAGCCG CAGCAGTTGT TGCGGTCGCG CAACAGCAGC AGGCTCAAGC CCAAGCCCAG 
GCTCAGGCTC AGGCACAGCA GCAGCAACAG GCGCCGCAGG TGGTGGTCCC CATGACCCCG 
CAGCACTTGA CCCCACAGCA GCAGCAGCAG AGCACACAGA GCATCGCCGA CTATCTGGCC 
CAGTTGCTCA AGGACCGCAA GCAACTGGCC GCCTTCCCCA ACGTCTTCAC CCACGTCGAA 
CGCCTGCTGG ACGAAGAAAT TGCACGCGTG CGCGCCTCAC TGTTCCAGAT CAATGGGGTC 
AAGAAGGAGC CGCTCACTCT GCCCGAACCC GAGGGCTCTG TGGTGACGAT GAACGAGAAG 
GTTTATGTGC CAGTCCGCGA GCATCCAGAT TTCAACTTTG TCGGTCGCAT TTTGGGACCC 
CGTGGCATGA CCGCCAAGCA ATTGGAACAG GAGACCGGCT GCAAGATTAT GGTCCGAGGC 
AAGGGTTCCA TGCGCGACAA GAAGAAGGAG GACGCCAACC GTGGCAAGCC TAACTGGGAG 
CATCTGTCCG ATGACCTGCA TGTCCTGATA ACCGTCGAGG ACACCGAGAA CCGTGCCACA 
GTGAAGTTGG CCCAGGCCGT CGCCGAAGTA CAGAAGTTGC TCGTGCCGCA AGCCGAAGGC 
GAAGATGAGC TAAAGAAACG TCAACTCATG GAATTGGCGA TTATTAATGG CACTTATAGG 
GACACAACAG CGAAATCTGT CGCAGTGTGC GATGAGGAGT GGCGCCGCCT GGTTGCCGCC 
TCTGATAGCC GCCTGCTGAC ATCCACCGGC CTGCCCGGCC TTGCCGCCCA GATCCGTGCA 
CCCGCCGCCG CCCCGCTTGG CGCCCCATTG ATCCTGAATC CCCGGATGAC CGTCCCCACA 
ACGGCGGCCA GCATATTGTC CGCCCAGGCC GCTCCGACAG CCGCCTTCGA CCAGACCGGC 
CATGGAATGA TCTTCGCACC GTACGATTAT GCGAACTACG CCGCCCTAGC CGGCAATCCT 
CTGCTCACGG AATATGCTGA TCATAGCGGT GGGTTGTTTG CCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

how ( NM_001275893.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262822.1 Drosophila melanogaster held out wings (how), transcript variant F, mRNA.

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CloneID	OFa08634
Clone ID Related Accession (Same CDS sequence)	NM_001275893.1
Accession Version	NM_001275893.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	held out wings, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGTCT GTGAGAGCAA AGCCGTTGTG CAACAGCAAC TGCAGCAGCA CTTGCAGCAA 
CAGGCAGCCG CAGCAGTTGT TGCGGTCGCG CAACAGCAGC AGGCTCAAGC CCAAGCCCAG 
GCTCAGGCTC AGGCACAGCA GCAGCAACAG GCGCCGCAGG TGGTGGTCCC CATGACCCCG 
CAGCACTTGA CCCCACAGCA GCAGCAGCAG AGCACACAGA GCATCGCCGA CTATCTGGCC 
CAGTTGCTCA AGGACCGCAA GCAACTGGCC GCCTTCCCCA ACGTCTTCAC CCACGTCGAA 
CGCCTGCTGG ACGAAGAAAT TGCACGCGTG CGCGCCTCAC TGTTCCAGAT CAATGGGGTC 
AAGAAGGAGC CGCTCACTCT GCCCGAACCC GAGGGCTCTG TGGTGACGAT GAACGAGAAG 
GTTTATGTGC CAGTCCGCGA GCATCCAGAT TTCAACTTTG TCGGTCGCAT TTTGGGACCC 
CGTGGCATGA CCGCCAAGCA ATTGGAACAG GAGACCGGCT GCAAGATTAT GGTCCGAGGC 
AAGGGTTCCA TGCGCGACAA GAAGAAGGAG GACGCCAACC GTGGCAAGCC TAACTGGGAG 
CATCTGTCCG ATGACCTGCA TGTCCTGATA ACCGTCGAGG ACACCGAGAA CCGTGCCACA 
GTGAAGTTGG CCCAGGCCGT CGCCGAAGTA CAGAAGTTGC TCGTGCCGCA AGCCGAAGGC 
GAAGATGAGC TAAAGAAACG TCAACTCATG GAATTGGCGA TTATTAATGG CACTTATAGG 
GACACAACAG CGAAATCTGT CGCAGCTTTC TCATGCGTTG GCTCTGCTTC TTATCTGTAT 
CCCGCAGTGT GCGATGAGGA GTGGCGCCGC CTGGTTGCCG CCTCTGATAG CCGCCTGCTG 
ACATCCACCG GCCTGCCCGG CCTTGCCGCC CAGATCCGTG CACCCGCCGC CGCCCCGCTT 
GGCGCCCCAT TGATCCTGAA TCCCCGGATG ACCGTCCCCA CAACGGCGGC CAGCATATTG 
TCCGCCCAGG CCGCTCCGAC AGCCGCCTTC GACCAGACCG GCCATGGAAT GATCTTCGCA 
CCGTACGATT ATGCGAACTA CGCCGCCCTA GCCGGCAATC CTCTGCTCAC GGAATATGCT 
GATCATAGCG GCGCCATTAA GCAGCAGCGT CGTTTGGCGA CTAACCGCGA GCATCCCTAT 
CAGCGAGCAA CGGTCGGAGT GCCAGCCAAG CCGGCGGGTT TCATTGAGAT ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262822.1
CDS	323..1579
Translation	MSVCESKAVVQQQLQQHLQQQAAAAVVAVAQQQQAQAQAQAQAQAQQQQQAPQVVVPMTPQHLTPQQQQQSTQSIADYLAQLLKDRKQLAAFPNVFTHVERLLDEEIARVRASLFQINGVKKEPLTLPEPEGSVVTMNEKVYVPVREHPDFNFVGRILGPRGMTAKQLEQETGCKIMVRGKGSMRDKKKEDANRGKPNWEHLSDDLHVLITVEDTENRATVKLAQAVAEVQKLLVPQAEGEDELKKRQLMELAIINGTYRDTTAKSVAAFSCVGSASYLYPAVCDEEWRRLVAASDSRLLTSTGLPGLAAQIRAPAAAPLGAPLILNPRMTVPTTAASILSAQAAPTAAFDQTGHGMIFAPYDYANYAALAGNPLLTEYADHSGAIKQQRRLATNREHPYQRATVGVPAKPAGFIEIQ

Target ORF information:

RefSeq Version	NM_001275893.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster held out wings (how), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275893.1

ORF Insert Sequence:

ATGAGTGTCT GTGAGAGCAA AGCCGTTGTG CAACAGCAAC TGCAGCAGCA CTTGCAGCAA 
CAGGCAGCCG CAGCAGTTGT TGCGGTCGCG CAACAGCAGC AGGCTCAAGC CCAAGCCCAG 
GCTCAGGCTC AGGCACAGCA GCAGCAACAG GCGCCGCAGG TGGTGGTCCC CATGACCCCG 
CAGCACTTGA CCCCACAGCA GCAGCAGCAG AGCACACAGA GCATCGCCGA CTATCTGGCC 
CAGTTGCTCA AGGACCGCAA GCAACTGGCC GCCTTCCCCA ACGTCTTCAC CCACGTCGAA 
CGCCTGCTGG ACGAAGAAAT TGCACGCGTG CGCGCCTCAC TGTTCCAGAT CAATGGGGTC 
AAGAAGGAGC CGCTCACTCT GCCCGAACCC GAGGGCTCTG TGGTGACGAT GAACGAGAAG 
GTTTATGTGC CAGTCCGCGA GCATCCAGAT TTCAACTTTG TCGGTCGCAT TTTGGGACCC 
CGTGGCATGA CCGCCAAGCA ATTGGAACAG GAGACCGGCT GCAAGATTAT GGTCCGAGGC 
AAGGGTTCCA TGCGCGACAA GAAGAAGGAG GACGCCAACC GTGGCAAGCC TAACTGGGAG 
CATCTGTCCG ATGACCTGCA TGTCCTGATA ACCGTCGAGG ACACCGAGAA CCGTGCCACA 
GTGAAGTTGG CCCAGGCCGT CGCCGAAGTA CAGAAGTTGC TCGTGCCGCA AGCCGAAGGC 
GAAGATGAGC TAAAGAAACG TCAACTCATG GAATTGGCGA TTATTAATGG CACTTATAGG 
GACACAACAG CGAAATCTGT CGCAGCTTTC TCATGCGTTG GCTCTGCTTC TTATCTGTAT 
CCCGCAGTGT GCGATGAGGA GTGGCGCCGC CTGGTTGCCG CCTCTGATAG CCGCCTGCTG 
ACATCCACCG GCCTGCCCGG CCTTGCCGCC CAGATCCGTG CACCCGCCGC CGCCCCGCTT 
GGCGCCCCAT TGATCCTGAA TCCCCGGATG ACCGTCCCCA CAACGGCGGC CAGCATATTG 
TCCGCCCAGG CCGCTCCGAC AGCCGCCTTC GACCAGACCG GCCATGGAAT GATCTTCGCA 
CCGTACGATT ATGCGAACTA CGCCGCCCTA GCCGGCAATC CTCTGCTCAC GGAATATGCT 
GATCATAGCG GCGCCATTAA GCAGCAGCGT CGTTTGGCGA CTAACCGCGA GCATCCCTAT 
CAGCGAGCAA CGGTCGGAGT GCCAGCCAAG CCGGCGGGTT TCATTGAGAT ACAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

how ( NM_001170213.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163684.1 Drosophila melanogaster held out wings (how), transcript variant E, mRNA.

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CloneID	OFa08635
Clone ID Related Accession (Same CDS sequence)	NM_001170213.2
Accession Version	NM_001170213.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	held out wings, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170213.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGTCT GTGAGAGCAA AGCCGTTGTG CAACAGCAAC TGCAGCAGCA CTTGCAGCAA 
CAGGCAGCCG CAGCAGTTGT TGCGGTCGCG CAACAGCAGC AGGCTCAAGC CCAAGCCCAG 
GCTCAGGCTC AGGCACAGCA GCAGCAACAG GCGCCGCAGG TGGTGGTCCC CATGACCCCG 
CAGCACTTGA CCCCACAGCA GCAGCAGCAG AGCACACAGA GCATCGCCGA CTATCTGGCC 
CAGTTGCTCA AGGACCGCAA GCAACTGGCC GCCTTCCCCA ACGTCTTCAC CCACGTCGAA 
CGCCTGCTGG ACGAAGAAAT TGCACGCGTG CGCGCCTCAC TGTTCCAGAT CAATGGGGTC 
AAGAAGGAGC CGCTCACTCT GCCCGAACCC GAGGGCTCTG TGGTGACGAT GAACGAGAAG 
GTTTATGTGC CAGTCCGCGA GCATCCAGAT TTCAACTTTG TCGGTCGCAT TTTGGGACCC 
CGTGGCATGA CCGCCAAGCA ATTGGAACAG GAGACCGGCT GCAAGATTAT GGTCCGAGGC 
AAGGGTTCCA TGCGCGACAA GAAGAAGGAG GACGCCAACC GTGGCAAGCC TAACTGGGAG 
CATCTGTCCG ATGACCTGCA TGTCCTGATA ACCGTCGAGG ACACCGAGAA CCGTGCCACA 
GTGAAGTTGG CCCAGGCCGT CGCCGAAGTA CAGAAGTTGC TCGTGCCGCA AGCCGAAGGC 
GAAGATGAGC TAAAGAAACG TCAACTCATG GAATTGGCGA TTATTAATGG CACTTATAGG 
GACACAACAG CGAAATCTGT CGCAGTGTGC GATGAGGAGT GGCGCCGCCT GGTTGCCGCC 
TCTGATAGCC GCCTGCTGAC ATCCACCGGC CTGCCCGGCC TTGCCGCCCA GATCCGTGCA 
CCCGCCGCCG CCCCGCTTGG CGCCCCATTG ATCCTGAATC CCCGGATGAC CGTCCCCACA 
ACGGCGGCCA GCATATTGTC CGCCCAGGCC GCTCCGACAG CCGCCTTCGA CCAGACCGGC 
CATGGAATGA TCTTCGCACC GTACGATTAT GCGAACTACG CCGCCCTAGC CGGCAATCCT 
CTGCTCACGG AATATGCTGA TCATAGCGGC GCCATTAAGC AGCAGCGTCG TTTGGCGACT 
AACCGCGAGC ATCCCTATCA GCGAGCAACG GTCGGAGTGC CAGCCAAGCC GGCGGGTTTC 
ATTGAGATAC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163684.1
CDS	323..1537
Translation	MSVCESKAVVQQQLQQHLQQQAAAAVVAVAQQQQAQAQAQAQAQAQQQQQAPQVVVPMTPQHLTPQQQQQSTQSIADYLAQLLKDRKQLAAFPNVFTHVERLLDEEIARVRASLFQINGVKKEPLTLPEPEGSVVTMNEKVYVPVREHPDFNFVGRILGPRGMTAKQLEQETGCKIMVRGKGSMRDKKKEDANRGKPNWEHLSDDLHVLITVEDTENRATVKLAQAVAEVQKLLVPQAEGEDELKKRQLMELAIINGTYRDTTAKSVAVCDEEWRRLVAASDSRLLTSTGLPGLAAQIRAPAAAPLGAPLILNPRMTVPTTAASILSAQAAPTAAFDQTGHGMIFAPYDYANYAALAGNPLLTEYADHSGAIKQQRRLATNREHPYQRATVGVPAKPAGFIEIQ

Target ORF information:

RefSeq Version	NM_001170213.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster held out wings (how), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170213.2

ORF Insert Sequence:

ATGAGTGTCT GTGAGAGCAA AGCCGTTGTG CAACAGCAAC TGCAGCAGCA CTTGCAGCAA 
CAGGCAGCCG CAGCAGTTGT TGCGGTCGCG CAACAGCAGC AGGCTCAAGC CCAAGCCCAG 
GCTCAGGCTC AGGCACAGCA GCAGCAACAG GCGCCGCAGG TGGTGGTCCC CATGACCCCG 
CAGCACTTGA CCCCACAGCA GCAGCAGCAG AGCACACAGA GCATCGCCGA CTATCTGGCC 
CAGTTGCTCA AGGACCGCAA GCAACTGGCC GCCTTCCCCA ACGTCTTCAC CCACGTCGAA 
CGCCTGCTGG ACGAAGAAAT TGCACGCGTG CGCGCCTCAC TGTTCCAGAT CAATGGGGTC 
AAGAAGGAGC CGCTCACTCT GCCCGAACCC GAGGGCTCTG TGGTGACGAT GAACGAGAAG 
GTTTATGTGC CAGTCCGCGA GCATCCAGAT TTCAACTTTG TCGGTCGCAT TTTGGGACCC 
CGTGGCATGA CCGCCAAGCA ATTGGAACAG GAGACCGGCT GCAAGATTAT GGTCCGAGGC 
AAGGGTTCCA TGCGCGACAA GAAGAAGGAG GACGCCAACC GTGGCAAGCC TAACTGGGAG 
CATCTGTCCG ATGACCTGCA TGTCCTGATA ACCGTCGAGG ACACCGAGAA CCGTGCCACA 
GTGAAGTTGG CCCAGGCCGT CGCCGAAGTA CAGAAGTTGC TCGTGCCGCA AGCCGAAGGC 
GAAGATGAGC TAAAGAAACG TCAACTCATG GAATTGGCGA TTATTAATGG CACTTATAGG 
GACACAACAG CGAAATCTGT CGCAGTGTGC GATGAGGAGT GGCGCCGCCT GGTTGCCGCC 
TCTGATAGCC GCCTGCTGAC ATCCACCGGC CTGCCCGGCC TTGCCGCCCA GATCCGTGCA 
CCCGCCGCCG CCCCGCTTGG CGCCCCATTG ATCCTGAATC CCCGGATGAC CGTCCCCACA 
ACGGCGGCCA GCATATTGTC CGCCCAGGCC GCTCCGACAG CCGCCTTCGA CCAGACCGGC 
CATGGAATGA TCTTCGCACC GTACGATTAT GCGAACTACG CCGCCCTAGC CGGCAATCCT 
CTGCTCACGG AATATGCTGA TCATAGCGGC GCCATTAAGC AGCAGCGTCG TTTGGCGACT 
AACCGCGAGC ATCCCTATCA GCGAGCAACG GTCGGAGTGC CAGCCAAGCC GGCGGGTTTC 
ATTGAGATAC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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how cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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