Cdk2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cdk2
Entrez Gene ID	42453
Full Name	Cyclin-dependent kinase 2
Synonyms	CDC2C,CDC2c,CDK2,CDK2/CDC2c,CG10498,Cdc2c,Dcdc2c,DmCdc2c,DmCdk2,Dmcdc2c,Dmel\CG10498,S(Sev-CycE)3A,cdc2c,cdk2,dCdk2
General protein information	Preferred Names Cyclin-dependent kinase 2 Names CG10498 gene product from transcript CG10498-RC CG10498-PA CG10498-PB CG10498-PC Cdk2-PA Cdk2-PB Cdk2-PC cdc2c cyclin E cyclin-dependent kinase 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 92F10-92F10

mRNA and Protein(s)

mRNA	Protein	Name
NM_169916.3	NP_732544.1	Cyclin-dependent kinase 2, isoform A
NM_001170195.3	NP_001163666.1	Cyclin-dependent kinase 2, isoform C
NM_079696.6	NP_524420.1	Cyclin-dependent kinase 2, isoform B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CDK2	XP_003952210.1
Neurospora crassa	NCU09778	XP_960117.1
Saccharomyces cerevisiae (baker's yeast)	CDC28	NP_009718.3
Oryza sativa (rice)	Os03g0118400	NP_001048772.1
Bos taurus (cattle)	CDK2	NP_001014934.1
Mus musculus (house mouse)	Cdk2	NP_904326.1
Rattus norvegicus (Norway rat)	Cdk2	NP_955795.1
Drosophila melanogaster (fruit fly)	cdc2c	NP_732544.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004579	XP_562342.4
Kluyveromyces lactis	KLLA0B09790g	XP_451964.1
Eremothecium gossypii	AGOS_ADR058C	NP_984154.1
Schizosaccharomyces pombe (fission yeast)	cdc2	NP_595629.1
Arabidopsis thaliana (thale cress)	CDC2	NP_566911.1
Xenopus tropicalis (tropical clawed frog)	cdk2	NP_001008136.1
Homo sapiens (human)	CDK2	NP_001789.2
	CDK2	XP_001113345.1
Canis lupus familiaris (dog)	CDK2	XP_005625536.1
Gallus gallus (chicken)	CDK2	NP_001186786.1
Magnaporthe oryzae (rice blast fungus)	MGG_01362	XP_003714294.1
Danio rerio (zebrafish)	cdk2	NP_998571.1
Oryza sativa (rice)	Os02g0123100	NP_001045731.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CycE and Cdk2 are required for germline stem cell proliferation.
Title: Cyclin E controls Drosophila female germline stem cell maintenance independently of its role in proliferation by modulating responsiveness to niche signals.

follicular stem cell function was substantially restored by expressing either excess DE-cadherin or excess E2F1/DP, the transcription factor normally activated by CycE-Cdk2 phosphorylation of retinoblastoma proteins
Title: Cyclin E-dependent protein kinase activity regulates niche retention of Drosophila ovarian follicle stem cells.

APC/C-Fzr activity is periodically inhibited by CycE-Cdk2, to prevent relicensing in S-phase cells.
Title: The anaphase-promoting complex/cyclosome (APC/C) is required for rereplication control in endoreplication cycles.

phosphorylation and degradation of Dup protein at G1/S requires cyclin E/CDK2
Title: Drosophila double-parked is sufficient to induce re-replication during development and is regulated by cyclin E/CDK2.

The following Cdk2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cdk2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05502	NM_001170195.3 Latest version!	Drosophila melanogaster Cyclin-dependent kinase 2 (Cdk2), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa05502	NM_169916.3 Latest version!	Drosophila melanogaster Cyclin-dependent kinase 2 (Cdk2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa05502	NM_079696.6 Latest version!	Drosophila melanogaster Cyclin-dependent kinase 2 (Cdk2), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cdk2 ( NM_169916.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732544.1 Drosophila melanogaster Cyclin-dependent kinase 2 (Cdk2), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa05502
Clone ID Related Accession (Same CDS sequence)	NM_169916.3 , NM_001170195.3 , NM_079696.6
Accession Version	NM_169916.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Cyclin-dependent kinase 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169916.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCACCA TTCTAGATAA CTTTCAACGC GCCGAAAAGA TTGGCGAGGG CACCTACGGT 
ATAGTTTACA AAGCGCGTAG CAACTCCACC GGCCAGGATG TGGCCCTCAA AAAGATTCGG 
CTAGAAGGCG AAACGGAGGG TGTTCCTTCG ACGGCCATTC GAGAGATCTC CCTGCTGAAG 
AACCTTAAGC ACCCAAATGT GGTCCAACTA TTTGACGTAG TCATTTCCGG CAACAATCTG 
TACATGATAT TCGAGTACCT GAACATGGAT CTAAAGAAGC TGATGGATAA GAAAAAAGAC 
GTGTTCACCC CTCAGTTGAT AAAGAGCTAT ATGCATCAGA TATTAGATGC CGTCGGCTTT 
TGCCACACGA ATCGTATCCT GCATCGCGAT CTCAAGCCCC AGAACCTTCT CGTAGACACG 
GCGGGCAAAA TAAAGTTGGC TGACTTTGGC CTAGCAAGGG CCTTCAACGT GCCTATGCGG 
GCGTACACAC ACGAAGTCGT CACCCTCTGG TACCGAGCTC CAGAGATTCT GTTGGGCACG 
AAATTCTACT CCACGGGCGT GGACATCTGG AGTCTAGGCT GCATTTTCTC TGAAATGATT 
ATGCGCCGCT CCTTGTTTCC TGGAGACAGC GAGATCGATC AACTTTATAG GATTTTCCGT 
ACCTTAAGCA CACCTGATGA AACAAATTGG CCTGGTGTGA CGCAGCTGCC AGACTTTAAG 
ACCAAGTTCC CTAGATGGGA GGGAACTAAC ATGCCACAAC CCATAACCGA ACACGAGGCG 
CACGAACTCA TAATGTCAAT GCTGTGCTAT GATCCCAACC TGCGCATCTC AGCCAAGGAC 
GCACTGCAGC ACGCTTACTT CCGCAATGTG CAGCATGTTG ACCATGTAGC CCTGCCTGTA 
GATCCCAATG CCGGCAGCGC TTCGCGTCTA ACGCGGCTCG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732544.1
CDS	75..1019
Translation	MTTILDNFQRAEKIGEGTYGIVYKARSNSTGQDVALKKIRLEGETEGVPSTAIREISLLKNLKHPNVVQLFDVVISGNNLYMIFEYLNMDLKKLMDKKKDVFTPQLIKSYMHQILDAVGFCHTNRILHRDLKPQNLLVDTAGKIKLADFGLARAFNVPMRAYTHEVVTLWYRAPEILLGTKFYSTGVDIWSLGCIFSEMIMRRSLFPGDSEIDQLYRIFRTLSTPDETNWPGVTQLPDFKTKFPRWEGTNMPQPITEHEAHELIMSMLCYDPNLRISAKDALQHAYFRNVQHVDHVALPVDPNAGSASRLTRLV

Target ORF information:

RefSeq Version	NM_169916.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Cyclin-dependent kinase 2 (Cdk2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169916.3

ORF Insert Sequence:

ATGACCACCA TTCTAGATAA CTTTCAACGC GCCGAAAAGA TTGGCGAGGG CACCTACGGT 
ATAGTTTACA AAGCGCGTAG CAACTCCACC GGCCAGGATG TGGCCCTCAA AAAGATTCGG 
CTAGAAGGCG AAACGGAGGG TGTTCCTTCG ACGGCCATTC GAGAGATCTC CCTGCTGAAG 
AACCTTAAGC ACCCAAATGT GGTCCAACTA TTTGACGTAG TCATTTCCGG CAACAATCTG 
TACATGATAT TCGAGTACCT GAACATGGAT CTAAAGAAGC TGATGGATAA GAAAAAAGAC 
GTGTTCACCC CTCAGTTGAT AAAGAGCTAT ATGCATCAGA TATTAGATGC CGTCGGCTTT 
TGCCACACGA ATCGTATCCT GCATCGCGAT CTCAAGCCCC AGAACCTTCT CGTAGACACG 
GCGGGCAAAA TAAAGTTGGC TGACTTTGGC CTAGCAAGGG CCTTCAACGT GCCTATGCGG 
GCGTACACAC ACGAAGTCGT CACCCTCTGG TACCGAGCTC CAGAGATTCT GTTGGGCACG 
AAATTCTACT CCACGGGCGT GGACATCTGG AGTCTAGGCT GCATTTTCTC TGAAATGATT 
ATGCGCCGCT CCTTGTTTCC TGGAGACAGC GAGATCGATC AACTTTATAG GATTTTCCGT 
ACCTTAAGCA CACCTGATGA AACAAATTGG CCTGGTGTGA CGCAGCTGCC AGACTTTAAG 
ACCAAGTTCC CTAGATGGGA GGGAACTAAC ATGCCACAAC CCATAACCGA ACACGAGGCG 
CACGAACTCA TAATGTCAAT GCTGTGCTAT GATCCCAACC TGCGCATCTC AGCCAAGGAC 
GCACTGCAGC ACGCTTACTT CCGCAATGTG CAGCATGTTG ACCATGTAGC CCTGCCTGTA 
GATCCCAATG CCGGCAGCGC TTCGCGTCTA ACGCGGCTCG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdk2 ( NM_001170195.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163666.1 Drosophila melanogaster Cyclin-dependent kinase 2 (Cdk2), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa05502
Clone ID Related Accession (Same CDS sequence)	NM_169916.3 , NM_001170195.3 , NM_079696.6
Accession Version	NM_001170195.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Cyclin-dependent kinase 2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001170195.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCACCA TTCTAGATAA CTTTCAACGC GCCGAAAAGA TTGGCGAGGG CACCTACGGT 
ATAGTTTACA AAGCGCGTAG CAACTCCACC GGCCAGGATG TGGCCCTCAA AAAGATTCGG 
CTAGAAGGCG AAACGGAGGG TGTTCCTTCG ACGGCCATTC GAGAGATCTC CCTGCTGAAG 
AACCTTAAGC ACCCAAATGT GGTCCAACTA TTTGACGTAG TCATTTCCGG CAACAATCTG 
TACATGATAT TCGAGTACCT GAACATGGAT CTAAAGAAGC TGATGGATAA GAAAAAAGAC 
GTGTTCACCC CTCAGTTGAT AAAGAGCTAT ATGCATCAGA TATTAGATGC CGTCGGCTTT 
TGCCACACGA ATCGTATCCT GCATCGCGAT CTCAAGCCCC AGAACCTTCT CGTAGACACG 
GCGGGCAAAA TAAAGTTGGC TGACTTTGGC CTAGCAAGGG CCTTCAACGT GCCTATGCGG 
GCGTACACAC ACGAAGTCGT CACCCTCTGG TACCGAGCTC CAGAGATTCT GTTGGGCACG 
AAATTCTACT CCACGGGCGT GGACATCTGG AGTCTAGGCT GCATTTTCTC TGAAATGATT 
ATGCGCCGCT CCTTGTTTCC TGGAGACAGC GAGATCGATC AACTTTATAG GATTTTCCGT 
ACCTTAAGCA CACCTGATGA AACAAATTGG CCTGGTGTGA CGCAGCTGCC AGACTTTAAG 
ACCAAGTTCC CTAGATGGGA GGGAACTAAC ATGCCACAAC CCATAACCGA ACACGAGGCG 
CACGAACTCA TAATGTCAAT GCTGTGCTAT GATCCCAACC TGCGCATCTC AGCCAAGGAC 
GCACTGCAGC ACGCTTACTT CCGCAATGTG CAGCATGTTG ACCATGTAGC CCTGCCTGTA 
GATCCCAATG CCGGCAGCGC TTCGCGTCTA ACGCGGCTCG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163666.1
CDS	75..1019
Translation	MTTILDNFQRAEKIGEGTYGIVYKARSNSTGQDVALKKIRLEGETEGVPSTAIREISLLKNLKHPNVVQLFDVVISGNNLYMIFEYLNMDLKKLMDKKKDVFTPQLIKSYMHQILDAVGFCHTNRILHRDLKPQNLLVDTAGKIKLADFGLARAFNVPMRAYTHEVVTLWYRAPEILLGTKFYSTGVDIWSLGCIFSEMIMRRSLFPGDSEIDQLYRIFRTLSTPDETNWPGVTQLPDFKTKFPRWEGTNMPQPITEHEAHELIMSMLCYDPNLRISAKDALQHAYFRNVQHVDHVALPVDPNAGSASRLTRLV

Target ORF information:

RefSeq Version	NM_001170195.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Cyclin-dependent kinase 2 (Cdk2), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170195.3

ORF Insert Sequence:

ATGACCACCA TTCTAGATAA CTTTCAACGC GCCGAAAAGA TTGGCGAGGG CACCTACGGT 
ATAGTTTACA AAGCGCGTAG CAACTCCACC GGCCAGGATG TGGCCCTCAA AAAGATTCGG 
CTAGAAGGCG AAACGGAGGG TGTTCCTTCG ACGGCCATTC GAGAGATCTC CCTGCTGAAG 
AACCTTAAGC ACCCAAATGT GGTCCAACTA TTTGACGTAG TCATTTCCGG CAACAATCTG 
TACATGATAT TCGAGTACCT GAACATGGAT CTAAAGAAGC TGATGGATAA GAAAAAAGAC 
GTGTTCACCC CTCAGTTGAT AAAGAGCTAT ATGCATCAGA TATTAGATGC CGTCGGCTTT 
TGCCACACGA ATCGTATCCT GCATCGCGAT CTCAAGCCCC AGAACCTTCT CGTAGACACG 
GCGGGCAAAA TAAAGTTGGC TGACTTTGGC CTAGCAAGGG CCTTCAACGT GCCTATGCGG 
GCGTACACAC ACGAAGTCGT CACCCTCTGG TACCGAGCTC CAGAGATTCT GTTGGGCACG 
AAATTCTACT CCACGGGCGT GGACATCTGG AGTCTAGGCT GCATTTTCTC TGAAATGATT 
ATGCGCCGCT CCTTGTTTCC TGGAGACAGC GAGATCGATC AACTTTATAG GATTTTCCGT 
ACCTTAAGCA CACCTGATGA AACAAATTGG CCTGGTGTGA CGCAGCTGCC AGACTTTAAG 
ACCAAGTTCC CTAGATGGGA GGGAACTAAC ATGCCACAAC CCATAACCGA ACACGAGGCG 
CACGAACTCA TAATGTCAAT GCTGTGCTAT GATCCCAACC TGCGCATCTC AGCCAAGGAC 
GCACTGCAGC ACGCTTACTT CCGCAATGTG CAGCATGTTG ACCATGTAGC CCTGCCTGTA 
GATCCCAATG CCGGCAGCGC TTCGCGTCTA ACGCGGCTCG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cdk2 ( NM_079696.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524420.1 Drosophila melanogaster Cyclin-dependent kinase 2 (Cdk2), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa05502
Clone ID Related Accession (Same CDS sequence)	NM_169916.3 , NM_001170195.3 , NM_079696.6
Accession Version	NM_079696.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Cyclin-dependent kinase 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079696.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCACCA TTCTAGATAA CTTTCAACGC GCCGAAAAGA TTGGCGAGGG CACCTACGGT 
ATAGTTTACA AAGCGCGTAG CAACTCCACC GGCCAGGATG TGGCCCTCAA AAAGATTCGG 
CTAGAAGGCG AAACGGAGGG TGTTCCTTCG ACGGCCATTC GAGAGATCTC CCTGCTGAAG 
AACCTTAAGC ACCCAAATGT GGTCCAACTA TTTGACGTAG TCATTTCCGG CAACAATCTG 
TACATGATAT TCGAGTACCT GAACATGGAT CTAAAGAAGC TGATGGATAA GAAAAAAGAC 
GTGTTCACCC CTCAGTTGAT AAAGAGCTAT ATGCATCAGA TATTAGATGC CGTCGGCTTT 
TGCCACACGA ATCGTATCCT GCATCGCGAT CTCAAGCCCC AGAACCTTCT CGTAGACACG 
GCGGGCAAAA TAAAGTTGGC TGACTTTGGC CTAGCAAGGG CCTTCAACGT GCCTATGCGG 
GCGTACACAC ACGAAGTCGT CACCCTCTGG TACCGAGCTC CAGAGATTCT GTTGGGCACG 
AAATTCTACT CCACGGGCGT GGACATCTGG AGTCTAGGCT GCATTTTCTC TGAAATGATT 
ATGCGCCGCT CCTTGTTTCC TGGAGACAGC GAGATCGATC AACTTTATAG GATTTTCCGT 
ACCTTAAGCA CACCTGATGA AACAAATTGG CCTGGTGTGA CGCAGCTGCC AGACTTTAAG 
ACCAAGTTCC CTAGATGGGA GGGAACTAAC ATGCCACAAC CCATAACCGA ACACGAGGCG 
CACGAACTCA TAATGTCAAT GCTGTGCTAT GATCCCAACC TGCGCATCTC AGCCAAGGAC 
GCACTGCAGC ACGCTTACTT CCGCAATGTG CAGCATGTTG ACCATGTAGC CCTGCCTGTA 
GATCCCAATG CCGGCAGCGC TTCGCGTCTA ACGCGGCTCG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524420.1
CDS	75..1019
Translation	MTTILDNFQRAEKIGEGTYGIVYKARSNSTGQDVALKKIRLEGETEGVPSTAIREISLLKNLKHPNVVQLFDVVISGNNLYMIFEYLNMDLKKLMDKKKDVFTPQLIKSYMHQILDAVGFCHTNRILHRDLKPQNLLVDTAGKIKLADFGLARAFNVPMRAYTHEVVTLWYRAPEILLGTKFYSTGVDIWSLGCIFSEMIMRRSLFPGDSEIDQLYRIFRTLSTPDETNWPGVTQLPDFKTKFPRWEGTNMPQPITEHEAHELIMSMLCYDPNLRISAKDALQHAYFRNVQHVDHVALPVDPNAGSASRLTRLV

Target ORF information:

RefSeq Version	NM_079696.6
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Cyclin-dependent kinase 2 (Cdk2), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079696.6

ORF Insert Sequence:

ATGACCACCA TTCTAGATAA CTTTCAACGC GCCGAAAAGA TTGGCGAGGG CACCTACGGT 
ATAGTTTACA AAGCGCGTAG CAACTCCACC GGCCAGGATG TGGCCCTCAA AAAGATTCGG 
CTAGAAGGCG AAACGGAGGG TGTTCCTTCG ACGGCCATTC GAGAGATCTC CCTGCTGAAG 
AACCTTAAGC ACCCAAATGT GGTCCAACTA TTTGACGTAG TCATTTCCGG CAACAATCTG 
TACATGATAT TCGAGTACCT GAACATGGAT CTAAAGAAGC TGATGGATAA GAAAAAAGAC 
GTGTTCACCC CTCAGTTGAT AAAGAGCTAT ATGCATCAGA TATTAGATGC CGTCGGCTTT 
TGCCACACGA ATCGTATCCT GCATCGCGAT CTCAAGCCCC AGAACCTTCT CGTAGACACG 
GCGGGCAAAA TAAAGTTGGC TGACTTTGGC CTAGCAAGGG CCTTCAACGT GCCTATGCGG 
GCGTACACAC ACGAAGTCGT CACCCTCTGG TACCGAGCTC CAGAGATTCT GTTGGGCACG 
AAATTCTACT CCACGGGCGT GGACATCTGG AGTCTAGGCT GCATTTTCTC TGAAATGATT 
ATGCGCCGCT CCTTGTTTCC TGGAGACAGC GAGATCGATC AACTTTATAG GATTTTCCGT 
ACCTTAAGCA CACCTGATGA AACAAATTGG CCTGGTGTGA CGCAGCTGCC AGACTTTAAG 
ACCAAGTTCC CTAGATGGGA GGGAACTAAC ATGCCACAAC CCATAACCGA ACACGAGGCG 
CACGAACTCA TAATGTCAAT GCTGTGCTAT GATCCCAACC TGCGCATCTC AGCCAAGGAC 
GCACTGCAGC ACGCTTACTT CCGCAATGTG CAGCATGTTG ACCATGTAGC CCTGCCTGTA 
GATCCCAATG CCGGCAGCGC TTCGCGTCTA ACGCGGCTCG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Cdk2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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