Ask1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following Ask1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ask1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa08538 NM_206518.2
Latest version!
Drosophila melanogaster apoptotic signal-regulating kinase 1, transcript variant B (Ask1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa08539 NM_057741.4
Latest version!
Drosophila melanogaster apoptotic signal-regulating kinase 1, transcript variant C (Ask1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa08538
    Clone ID Related Accession (Same CDS sequence) NM_206518.2
    Accession Version NM_206518.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3591bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product apoptotic signal-regulating kinase 1, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_206518.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCGTGAGA AATTTCTGAG TGACATGCGA ACGGCGCGGG ACACGTACGC CACCAACGGC 
    GCCAAGCTGC AGAGTATATT GCACGAGATG CGAAAGCGGC TGGACGATCC GCATGTCCTG
    TCTGGCGAAG TGGTACACAG TTTCATGTGC TCGCTGAGGG ATGTGCAGGA CTACGATGCC
    ATGGTGCGAC TGGTCAATGA CCTGAAGAAC ATACCCAACA CCCGGAAGTA CGTGGAGACG
    GGCAACATGA GTTTCCTGTA CGCCTTCGCC CTGAACCGAA GAAATCGAAA GGGAGACCGT
    GAAAAGGCTC TGGAATCTTC GCTGAAGGCG CTGGAAAAGA AGGAGAACCA CTTTCCGGAC
    ATGCTCTGCC TGTGCGGCCG CATCTACAAG GACATCTTTG TGGAATCCGA CTACACGGAC
    GCCACCAGTT TGGCGCACGC CATCAAGTGG TACCGCCAAA GCTTTGAGGT GCAGCCGAAT
    GAGTATGCAG GCATCAATCT GGCTACTCTG CTGGTTATCG AGGGCAAGGA GTTTACCAAT
    ACGGAGGAGT TGCAGCACAT CGGCATGACC TTAAACAATT TAATCGGTAA AAAGGGCAGC
    CTATCCTCGC TGAGTGAGTA TTGGGATGTG GCCACCTTCT TTGAGATCTC CGTTTTGGCC
    AACGACTACG CCAAGGCGAT TCAAGCTGCC GAGTGCATGT TCAAGCTAAA GCCACCCAAC
    TGGTATCTGA AGTCGACCAT CGGAAATATC TCTCTTATAC ATCGATTTCG AAAGAAGCCC
    GAGGAACGCC AGCCGCCCAT CGAGGAGCAG GTGTTCCAGT TCTGGATGGA CTTCTTTCTG
    GAGGCCACCA ACACCGAGGA GGTTAAGAAC AGCATTCGCT TTCCGATTCT GATCCTCGAG
    CCTCAGAAGA TTTATATGCC CAGTTATGTG ACTATAAACA TGGATGCGGA TGAGAAGTCC
    ATTCAGATTG TGAACATCTG TTTGGCTCAC GCAAAGAATG CGTGTAAGAA AATCCACGAC
    TTCCTTTTCG TGGCATCGAA GATCAAGTCC GTCAGCCTTT ACAAGAGGGA TGATCGTTGT
    GCCTACTTGT ACGTCCATCA CAACTCAGAT GATTTTCAAA TATATTTTCC CTCCACTGAA
    CGACGACAAA AGTTCTACGA TATGATCCTG GAGATGACTG CAGATCAGGT GGTGTTTGTC
    AATCTCAGCA ATGATGACGC TAATATCGAG TACGAGTACG ACTACGATGA GCAGAATCGC
    AAAATGGTGC TGGGCAAAGG TACTTATGGC ACCGTGTACG CCGCCAGGGA CAAGCAGACG
    CAGGTGCGAA TCGCCATTAA GGAGGTGCCG GAAAAGAACT CGCAGGATGT GCAGCCACTG
    CACGAGGAGA TTAAGTTACA CTCTCAGCTG CGACATCGCA ACATAGTTCG CTATTTGGGA
    TCGTGCTCGG AGAACGGCTT CTTCAAGATC TTCATGGAGC AGGTGCCGGG AGGATCGCTT
    TCCGACCTCT TAGAGACCAA ATGGGGTCCG CTCAAGGACA ACGAGTCCAC CATGGCCTTC
    TACTCCAAGC AGATCCTCGA GGGGCTGAAG TACCTTCACG AGCAGGACAT TGTTCATCGC
    GATATAAAGG GTGACAACGT GCTGGTTAAC ACGTATAGCG GCGTGGTTAA GATCTCCGAT
    TTCGGCACCT CCAAGCGGCT GGCCCGCATC AACCCGATGA CGGAGACCTT TACGGGCACC
    TTGCAGTACA TGGCGCCGGA GGTGATTGAC CAGGGAGTAC GCGGCTATGG CCCGGCGGCT
    GACATCTGGT CCTTTGGATG CACAAACGTT GAGATGGCTA CGGGCAAACC ACCGTTTATC
    GAGCTGGGTA GCGCCCATGC GGCCATGTTC AAGGTGGGCT TTTACAAGAA GCATCCGAAC
    ATACCCGAGG AGCTGTCCGC CAACGCAAAG AACTTTATTT TGCGTTGCTT TGCCATCAGC
    GTGATGGACC GACCCTCCGC CTCGCAACTT CTGGAAGATC CCTTCCTACA AGATAAACCC
    CGCAAGGTGC GCCCAGCCCT GCCCATCAAT ACTGAGTTCG GACGCAGCAT TTCGGTGCCG
    GCTGATCGGT TTGTGCACAA AACCACACCG CCGCTGAGCT ACAACACAAC CTGCAATACG
    CCCACAACGC CGGAATTGGA CATAACGCAT TCATCCTCGG TGGACATTGA CGAGCTGCCC
    AGCAACCAGT TTATGCTTGA ACGGCGAAAT TCCCATGGAT TTTTGCTCTC GCCAGAGATT
    GAACCTTCCA CGCCGTCCTT GCGTACGAGC ATTAGTGAGA CGAGCGAGAC TGACGGTTTT
    TACAGGCTTA AAAAGGATTC GCAGAGGCGG ACAACGCTTT CCAAGGTGCT GGCACTAGAC
    GAATCCAAGA TCTGCGATAT TTGGCTGAAG AAGGTCGACG CTGATCAGAA CTCGATTGCG
    ATTAGGAAAA GCGATTTGGA GGTTCTAATC CGAGGACTGC GGGACTACAT TATGAACGAA
    AACGAAAAGC ATTTGGAAGC GACTATCAAC GAACTGAAAC AAAAGCTGAA CAACGACGCA
    GTTGCTCTGG ACCATCTTCA TTTGGCGCTG TACTCCTTTC AGGACGCCGT GGTCTGCGTA
    CTGCGGTACC ATTGCATCAA ACCCCATTGG ATGTTCGCAT TGGATAATCT GGTTAAGCGG
    GCCGTCCAGG CGGCGGTCAC TATTTTCTCT CCTGAACTGG GCGCCAATCT GGCGGACAAA
    GACCTGTCTG GAAATGATGA TGAGAGCTTG CGTACAAGTC TAATGCAGAA GGGTTCCACC
    GAGAGCCAGG AAAAGCAGAG CCTGGAAAAA GTTCTTCCCA CGAGCAACAC TCGCGAAGGT
    GTGGGATTAA CGCCTGCGGA ATCGGGCGGG GATGCTCTGT CCAGTTCGAT GGAGTGCGCG
    CGCATCCTGA GAGACATCCG TGACAATCAG CAGAAGCTGC AACGCCAGCT CCTTGAGCAG
    CAGCGGCAGA GCATGAGGGC CCTGCATAAT ATCAGTCAGG AGCTGGCTCA CATTTACATG
    GGCGGCAGAA AGAGATTAAG ACGCACAATA AACGGCTTTA ACAAAAAGTG CCATAGAATG
    AGTACCAGTG TGGGCGGAAC GTCCGGAGTC CGTCCGAATA TAGCGGGCAA TCCCCGGAGG
    CAGCTAAACA AGCATCACTT TGGTCTTCAC GAAGTAGACG AGCAGTTGGA GCAGTGGTTG
    ACCCAGCAGG AAATAGACGA GTTCTCAAAG ACGCTCATCC TGAATGAAGG TTTTACATTT
    GACGACTTTA TCTATAACAT GGAAAAGCTG GACCTCATGA GGCTGGGTCT GCGCGTTGGC
    ATCGAAGTGC GTTTGTGGAA ACTCATTATA CAAGAACGTG TGTGCACCTC GTCCGATTGT
    GTGGACAGTC CACCAAGCAC TTACCACAAA CCCATTGGAG GCAATAGCTC TTTTGCGCTT
    ATTGACAACA ACAATACGCT ACCATCACCC ACAACCACAA CTCCCGGAAC GCCGGGCATC
    AAAACGAAAA TCAAGAAAAG CGAAAGCGAG TACGATTCGT GCAGCGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_996240.1
    CDS1279..4869
    Translation

    Target ORF information:

    RefSeq Version NM_206518.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster apoptotic signal-regulating kinase 1, transcript variant B (Ask1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_206518.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCGTGAGA AATTTCTGAG TGACATGCGA ACGGCGCGGG ACACGTACGC CACCAACGGC 
    GCCAAGCTGC AGAGTATATT GCACGAGATG CGAAAGCGGC TGGACGATCC GCATGTCCTG
    TCTGGCGAAG TGGTACACAG TTTCATGTGC TCGCTGAGGG ATGTGCAGGA CTACGATGCC
    ATGGTGCGAC TGGTCAATGA CCTGAAGAAC ATACCCAACA CCCGGAAGTA CGTGGAGACG
    GGCAACATGA GTTTCCTGTA CGCCTTCGCC CTGAACCGAA GAAATCGAAA GGGAGACCGT
    GAAAAGGCTC TGGAATCTTC GCTGAAGGCG CTGGAAAAGA AGGAGAACCA CTTTCCGGAC
    ATGCTCTGCC TGTGCGGCCG CATCTACAAG GACATCTTTG TGGAATCCGA CTACACGGAC
    GCCACCAGTT TGGCGCACGC CATCAAGTGG TACCGCCAAA GCTTTGAGGT GCAGCCGAAT
    GAGTATGCAG GCATCAATCT GGCTACTCTG CTGGTTATCG AGGGCAAGGA GTTTACCAAT
    ACGGAGGAGT TGCAGCACAT CGGCATGACC TTAAACAATT TAATCGGTAA AAAGGGCAGC
    CTATCCTCGC TGAGTGAGTA TTGGGATGTG GCCACCTTCT TTGAGATCTC CGTTTTGGCC
    AACGACTACG CCAAGGCGAT TCAAGCTGCC GAGTGCATGT TCAAGCTAAA GCCACCCAAC
    TGGTATCTGA AGTCGACCAT CGGAAATATC TCTCTTATAC ATCGATTTCG AAAGAAGCCC
    GAGGAACGCC AGCCGCCCAT CGAGGAGCAG GTGTTCCAGT TCTGGATGGA CTTCTTTCTG
    GAGGCCACCA ACACCGAGGA GGTTAAGAAC AGCATTCGCT TTCCGATTCT GATCCTCGAG
    CCTCAGAAGA TTTATATGCC CAGTTATGTG ACTATAAACA TGGATGCGGA TGAGAAGTCC
    ATTCAGATTG TGAACATCTG TTTGGCTCAC GCAAAGAATG CGTGTAAGAA AATCCACGAC
    TTCCTTTTCG TGGCATCGAA GATCAAGTCC GTCAGCCTTT ACAAGAGGGA TGATCGTTGT
    GCCTACTTGT ACGTCCATCA CAACTCAGAT GATTTTCAAA TATATTTTCC CTCCACTGAA
    CGACGACAAA AGTTCTACGA TATGATCCTG GAGATGACTG CAGATCAGGT GGTGTTTGTC
    AATCTCAGCA ATGATGACGC TAATATCGAG TACGAGTACG ACTACGATGA GCAGAATCGC
    AAAATGGTGC TGGGCAAAGG TACTTATGGC ACCGTGTACG CCGCCAGGGA CAAGCAGACG
    CAGGTGCGAA TCGCCATTAA GGAGGTGCCG GAAAAGAACT CGCAGGATGT GCAGCCACTG
    CACGAGGAGA TTAAGTTACA CTCTCAGCTG CGACATCGCA ACATAGTTCG CTATTTGGGA
    TCGTGCTCGG AGAACGGCTT CTTCAAGATC TTCATGGAGC AGGTGCCGGG AGGATCGCTT
    TCCGACCTCT TAGAGACCAA ATGGGGTCCG CTCAAGGACA ACGAGTCCAC CATGGCCTTC
    TACTCCAAGC AGATCCTCGA GGGGCTGAAG TACCTTCACG AGCAGGACAT TGTTCATCGC
    GATATAAAGG GTGACAACGT GCTGGTTAAC ACGTATAGCG GCGTGGTTAA GATCTCCGAT
    TTCGGCACCT CCAAGCGGCT GGCCCGCATC AACCCGATGA CGGAGACCTT TACGGGCACC
    TTGCAGTACA TGGCGCCGGA GGTGATTGAC CAGGGAGTAC GCGGCTATGG CCCGGCGGCT
    GACATCTGGT CCTTTGGATG CACAAACGTT GAGATGGCTA CGGGCAAACC ACCGTTTATC
    GAGCTGGGTA GCGCCCATGC GGCCATGTTC AAGGTGGGCT TTTACAAGAA GCATCCGAAC
    ATACCCGAGG AGCTGTCCGC CAACGCAAAG AACTTTATTT TGCGTTGCTT TGCCATCAGC
    GTGATGGACC GACCCTCCGC CTCGCAACTT CTGGAAGATC CCTTCCTACA AGATAAACCC
    CGCAAGGTGC GCCCAGCCCT GCCCATCAAT ACTGAGTTCG GACGCAGCAT TTCGGTGCCG
    GCTGATCGGT TTGTGCACAA AACCACACCG CCGCTGAGCT ACAACACAAC CTGCAATACG
    CCCACAACGC CGGAATTGGA CATAACGCAT TCATCCTCGG TGGACATTGA CGAGCTGCCC
    AGCAACCAGT TTATGCTTGA ACGGCGAAAT TCCCATGGAT TTTTGCTCTC GCCAGAGATT
    GAACCTTCCA CGCCGTCCTT GCGTACGAGC ATTAGTGAGA CGAGCGAGAC TGACGGTTTT
    TACAGGCTTA AAAAGGATTC GCAGAGGCGG ACAACGCTTT CCAAGGTGCT GGCACTAGAC
    GAATCCAAGA TCTGCGATAT TTGGCTGAAG AAGGTCGACG CTGATCAGAA CTCGATTGCG
    ATTAGGAAAA GCGATTTGGA GGTTCTAATC CGAGGACTGC GGGACTACAT TATGAACGAA
    AACGAAAAGC ATTTGGAAGC GACTATCAAC GAACTGAAAC AAAAGCTGAA CAACGACGCA
    GTTGCTCTGG ACCATCTTCA TTTGGCGCTG TACTCCTTTC AGGACGCCGT GGTCTGCGTA
    CTGCGGTACC ATTGCATCAA ACCCCATTGG ATGTTCGCAT TGGATAATCT GGTTAAGCGG
    GCCGTCCAGG CGGCGGTCAC TATTTTCTCT CCTGAACTGG GCGCCAATCT GGCGGACAAA
    GACCTGTCTG GAAATGATGA TGAGAGCTTG CGTACAAGTC TAATGCAGAA GGGTTCCACC
    GAGAGCCAGG AAAAGCAGAG CCTGGAAAAA GTTCTTCCCA CGAGCAACAC TCGCGAAGGT
    GTGGGATTAA CGCCTGCGGA ATCGGGCGGG GATGCTCTGT CCAGTTCGAT GGAGTGCGCG
    CGCATCCTGA GAGACATCCG TGACAATCAG CAGAAGCTGC AACGCCAGCT CCTTGAGCAG
    CAGCGGCAGA GCATGAGGGC CCTGCATAAT ATCAGTCAGG AGCTGGCTCA CATTTACATG
    GGCGGCAGAA AGAGATTAAG ACGCACAATA AACGGCTTTA ACAAAAAGTG CCATAGAATG
    AGTACCAGTG TGGGCGGAAC GTCCGGAGTC CGTCCGAATA TAGCGGGCAA TCCCCGGAGG
    CAGCTAAACA AGCATCACTT TGGTCTTCAC GAAGTAGACG AGCAGTTGGA GCAGTGGTTG
    ACCCAGCAGG AAATAGACGA GTTCTCAAAG ACGCTCATCC TGAATGAAGG TTTTACATTT
    GACGACTTTA TCTATAACAT GGAAAAGCTG GACCTCATGA GGCTGGGTCT GCGCGTTGGC
    ATCGAAGTGC GTTTGTGGAA ACTCATTATA CAAGAACGTG TGTGCACCTC GTCCGATTGT
    GTGGACAGTC CACCAAGCAC TTACCACAAA CCCATTGGAG GCAATAGCTC TTTTGCGCTT
    ATTGACAACA ACAATACGCT ACCATCACCC ACAACCACAA CTCCCGGAAC GCCGGGCATC
    AAAACGAAAA TCAAGAAAAG CGAAAGCGAG TACGATTCGT GCAGCGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08539
    Clone ID Related Accession (Same CDS sequence) NM_057741.4
    Accession Version NM_057741.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product apoptotic signal-regulating kinase 1, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_057741.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGATGTGG TGTGTGTAAT GGATACGGCG GTGGGCGACC ATCTGGAGGA CCGCCAGTGT 
    GCGCTGGAGG AGATCAAGCA GGCAGTGCAG TCGGCGGGCG CCAACTTTCA GCGGGTTCAG
    TTCGAGCGCC TGGACTTCGG CGAGACGAAT GTGCTGGAGA CGTTTTACAA CGCCGATGTG
    GCCATTATCG ACCTGAGCAT TCTCACCCAG CAGCGGCCGC TTTCCTATCA CTACGGCGTT
    AGGGAGAGCT TCGGGATGAA GGAGAACATC CTCACCTACA ACGACATCGA TTCCAAGCAG
    ACGCTCAGTC TCAAGCTCTC GTGTGCCAAC TACCTGTTTC TCAGCTACAA GCGGAATGCG
    GAGACCAATT CCTGCCACCT GACCAGCCAG CCCAACAGCG GCAACAACAG CAAGGAACCC
    AATGCCGAAG GACGAGTGCC CACTTTGCAG TGGCGCCTCA AGCGAAAGCT GCAAGATGTT
    GAGATCCAGT CGAAAGCCCA CATGCGTGAG AAATTTCTGA GTGACATGCG AACGGCGCGG
    GACACGTACG CCACCAACGG CGCCAAGCTG CAGAGTATAT TGCACGAGAT GCGAAAGCGG
    CTGGACGATC CGCATGTCCT GTCTGGCGAA GTGGTACACA GTTTCATGTG CTCGCTGAGG
    GATGTGCAGG ACTACGATGC CATGGTGCGA CTGGTCAATG ACCTGAAGAA CATACCCAAC
    ACCCGGAAGT ACGTGGAGAC GGGCAACATG AGTTTCCTGT ACGCCTTCGC CCTGAACCGA
    AGAAATCGAA AGGGAGACCG TGAAAAGGCT CTGGAATCTT CGCTGAAGGC GCTGGAAAAG
    AAGGAGAACC ACTTTCCGGA CATGCTCTGC CTGTGCGGCC GCATCTACAA GGACATCTTT
    GTGGAATCCG ACTACACGGA CGCCACCAGT TTGGCGCACG CCATCAAGTG GTACCGCCAA
    AGCTTTGAGG TGCAGCCGAA TGAGTATGCA GGCATCAATC TGGCTACTCT GCTGGTTATC
    GAGGGCAAGG AGTTTACCAA TACGGAGGAG TTGCAGCACA TCGGCATGAC CTTAAACAAT
    TTAATCGGTA AAAAGGGCAG CCTATCCTCG CTGAGTGAGT ATTGGGATGT GGCCACCTTC
    TTTGAGATCT CCGTTTTGGC CAACGACTAC GCCAAGGCGA TTCAAGCTGC CGAGTGCATG
    TTCAAGCTAA AGCCACCCAA CTGGTATCTG AAGTCGACCA TCGGAAATAT CTCTCTTATA
    CATCGATTTC GAAAGAAGCC CGAGGAACGC CAGCCGCCCA TCGAGGAGCA GGTGTTCCAG
    TTCTGGATGG ACTTCTTTCT GGAGGCCACC AACACCGAGG AGGTTAAGAA CAGCATTCGC
    TTTCCGATTC TGATCCTCGA GCCTCAGAAG ATTTATATGC CCAGTTATGT GACTATAAAC
    ATGGATGCGG ATGAGAAGTC CATTCAGATT GTGAACATCT GTTTGGCTCA CGCAAAGAAT
    GCGTGTAAGA AAATCCACGA CTTCCTTTTC GTGGCATCGA AGATCAAGTC CGTCAGCCTT
    TACAAGAGGG ATGATCGTTG TGCCTACTTG TACGTCCATC ACAACTCAGA TGATTTTCAA
    ATATATTTTC CCTCCACTGA ACGACGACAA AAGTTCTACG ATATGATCCT GGAGATGACT
    GCAGATCAGG TGGTGTTTGT CAATCTCAGC AATGATGACG CTAATATCGA GTACGAGTAC
    GACTACGATG AGCAGAATCG CAAAATGGTG CTGGGCAAAG GTACTTATGG CACCGTGTAC
    GCCGCCAGGG ACAAGCAGAC GCAGGTGCGA ATCGCCATTA AGGAGGTGCC GGAAAAGAAC
    TCGCAGGATG TGCAGCCACT GCACGAGGAG ATTAAGTTAC ACTCTCAGCT GCGACATCGC
    AACATAGTTC GCTATTTGGG ATCGTGCTCG GAGAACGGCT TCTTCAAGAT CTTCATGGAG
    CAGGTGCCGG GAGGATCGCT TTCCGACCTC TTAGAGACCA AATGGGGTCC GCTCAAGGAC
    AACGAGTCCA CCATGGCCTT CTACTCCAAG CAGATCCTCG AGGGGCTGAA GTACCTTCAC
    GAGCAGGACA TTGTTCATCG CGATATAAAG GGTGACAACG TGCTGGTTAA CACGTATAGC
    GGCGTGGTTA AGATCTCCGA TTTCGGCACC TCCAAGCGGC TGGCCCGCAT CAACCCGATG
    ACGGAGACCT TTACGGGCAC CTTGCAGTAC ATGGCGCCGG AGGTGATTGA CCAGGGAGTA
    CGCGGCTATG GCCCGGCGGC TGACATCTGG TCCTTTGGAT GCACAAACGT TGAGATGGCT
    ACGGGCAAAC CACCGTTTAT CGAGCTGGGT AGCGCCCATG CGGCCATGTT CAAGGTGGGC
    TTTTACAAGA AGCATCCGAA CATACCCGAG GAGCTGTCCG CCAACGCAAA GAACTTTATT
    TTGCGTTGCT TTGCCATCAG CGTGATGGAC CGACCCTCCG CCTCGCAACT TCTGGAAGAT
    CCCTTCCTAC AAGATAAACC CCGCAAGGTG CGCCCAGCCC TGCCCATCAA TACTGAGTTC
    GGACGCAGCA TTTCGGTGCC GGCTGATCGG TTTGTGCACA AAACCACACC GCCGCTGAGC
    TACAACACAA CCTGCAATAC GCCCACAACG CCGGAATTGG ACATAACGCA TTCATCCTCG
    GTGGACATTG ACGAGCTGCC CAGCAACCAG TTTATGCTTG AACGGCGAAA TTCCCATGGA
    TTTTTGCTCT CGCCAGAGAT TGAACCTTCC ACGCCGTCCT TGCGTACGAG CATTAGTGAG
    ACGAGCGAGA CTGACGGTTT TTACAGGCTT AAAAAGGATT CGCAGAGGCG GACAACGCTT
    TCCAAGGTGC TGGCACTAGA CGAATCCAAG ATCTGCGATA TTTGGCTGAA GAAGGTCGAC
    GCTGATCAGA ACTCGATTGC GATTAGGAAA AGCGATTTGG AGGTTCTAAT CCGAGGACTG
    CGGGACTACA TTATGAACGA AAACGAAAAG CATTTGGAAG CGACTATCAA CGAACTGAAA
    CAAAAGCTGA ACAACGACGC AGTTGCTCTG GACCATCTTC ATTTGGCGCT GTACTCCTTT
    CAGGACGCCG TGGTCTGCGT ACTGCGGTAC CATTGCATCA AACCCCATTG GATGTTCGCA
    TTGGATAATC TGGTTAAGCG GGCCGTCCAG GCGGCGGTCA CTATTTTCTC TCCTGAACTG
    GGCGCCAATC TGGCGGACAA AGACCTGTCT GGAAATGATG ATGAGAGCTT GCGTACAAGT
    CTAATGCAGA AGGGTTCCAC CGAGAGCCAG GAAAAGCAGA GCCTGGAAAA AGTTCTTCCC
    ACGAGCAACA CTCGCGAAGG TGTGGGATTA ACGCCTGCGG AATCGGGCGG GGATGCTCTG
    TCCAGTTCGA TGGAGTGCGC GCGCATCCTG AGAGACATCC GTGACAATCA GCAGAAGCTG
    CAACGCCAGC TCCTTGAGCA GCAGCGGCAG AGCATGAGGG CCCTGCATAA TATCAGTCAG
    GAGCTGGCTC ACATTTACAT GGGCGGCAGA AAGAGATTAA GACGCACAAT AAACGGCTTT
    AACAAAAAGT GCCATAGAAT GAGTACCAGT GTGGGCGGAA CGTCCGGAGT CCGTCCGAAT
    ATAGCGGGCA ATCCCCGGAG GCAGCTAAAC AAGCATCACT TTGGTCTTCA CGAAGTAGAC
    GAGCAGTTGG AGCAGTGGTT GACCCAGCAG GAAATAGACG AGTTCTCAAA GACGCTCATC
    CTGAATGAAG GTTTTACATT TGACGACTTT ATCTATAACA TGGAAAAGCT GGACCTCATG
    AGGCTGGGTC TGCGCGTTGG CATCGAAGTG CGTTTGTGGA AACTCATTAT ACAAGAACGT
    GTGTGCACCT CGTCCGATTG TGTGGACAGT CCACCAAGCA CTTACCACAA ACCCATTGGA
    GGCAATAGCT CTTTTGCGCT TATTGACAAC AACAATACGC TACCATCACC CACAACCACA
    ACTCCCGGAA CGCCGGGCAT CAAAACGAAA ATCAAGAAAA GCGAAAGCGA GTACGATTCG
    TGCAGCGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_477089.3
    CDS206..4297
    Translation

    Target ORF information:

    RefSeq Version NM_057741.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster apoptotic signal-regulating kinase 1, transcript variant C (Ask1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_057741.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGATGTGG TGTGTGTAAT GGATACGGCG GTGGGCGACC ATCTGGAGGA CCGCCAGTGT 
    GCGCTGGAGG AGATCAAGCA GGCAGTGCAG TCGGCGGGCG CCAACTTTCA GCGGGTTCAG
    TTCGAGCGCC TGGACTTCGG CGAGACGAAT GTGCTGGAGA CGTTTTACAA CGCCGATGTG
    GCCATTATCG ACCTGAGCAT TCTCACCCAG CAGCGGCCGC TTTCCTATCA CTACGGCGTT
    AGGGAGAGCT TCGGGATGAA GGAGAACATC CTCACCTACA ACGACATCGA TTCCAAGCAG
    ACGCTCAGTC TCAAGCTCTC GTGTGCCAAC TACCTGTTTC TCAGCTACAA GCGGAATGCG
    GAGACCAATT CCTGCCACCT GACCAGCCAG CCCAACAGCG GCAACAACAG CAAGGAACCC
    AATGCCGAAG GACGAGTGCC CACTTTGCAG TGGCGCCTCA AGCGAAAGCT GCAAGATGTT
    GAGATCCAGT CGAAAGCCCA CATGCGTGAG AAATTTCTGA GTGACATGCG AACGGCGCGG
    GACACGTACG CCACCAACGG CGCCAAGCTG CAGAGTATAT TGCACGAGAT GCGAAAGCGG
    CTGGACGATC CGCATGTCCT GTCTGGCGAA GTGGTACACA GTTTCATGTG CTCGCTGAGG
    GATGTGCAGG ACTACGATGC CATGGTGCGA CTGGTCAATG ACCTGAAGAA CATACCCAAC
    ACCCGGAAGT ACGTGGAGAC GGGCAACATG AGTTTCCTGT ACGCCTTCGC CCTGAACCGA
    AGAAATCGAA AGGGAGACCG TGAAAAGGCT CTGGAATCTT CGCTGAAGGC GCTGGAAAAG
    AAGGAGAACC ACTTTCCGGA CATGCTCTGC CTGTGCGGCC GCATCTACAA GGACATCTTT
    GTGGAATCCG ACTACACGGA CGCCACCAGT TTGGCGCACG CCATCAAGTG GTACCGCCAA
    AGCTTTGAGG TGCAGCCGAA TGAGTATGCA GGCATCAATC TGGCTACTCT GCTGGTTATC
    GAGGGCAAGG AGTTTACCAA TACGGAGGAG TTGCAGCACA TCGGCATGAC CTTAAACAAT
    TTAATCGGTA AAAAGGGCAG CCTATCCTCG CTGAGTGAGT ATTGGGATGT GGCCACCTTC
    TTTGAGATCT CCGTTTTGGC CAACGACTAC GCCAAGGCGA TTCAAGCTGC CGAGTGCATG
    TTCAAGCTAA AGCCACCCAA CTGGTATCTG AAGTCGACCA TCGGAAATAT CTCTCTTATA
    CATCGATTTC GAAAGAAGCC CGAGGAACGC CAGCCGCCCA TCGAGGAGCA GGTGTTCCAG
    TTCTGGATGG ACTTCTTTCT GGAGGCCACC AACACCGAGG AGGTTAAGAA CAGCATTCGC
    TTTCCGATTC TGATCCTCGA GCCTCAGAAG ATTTATATGC CCAGTTATGT GACTATAAAC
    ATGGATGCGG ATGAGAAGTC CATTCAGATT GTGAACATCT GTTTGGCTCA CGCAAAGAAT
    GCGTGTAAGA AAATCCACGA CTTCCTTTTC GTGGCATCGA AGATCAAGTC CGTCAGCCTT
    TACAAGAGGG ATGATCGTTG TGCCTACTTG TACGTCCATC ACAACTCAGA TGATTTTCAA
    ATATATTTTC CCTCCACTGA ACGACGACAA AAGTTCTACG ATATGATCCT GGAGATGACT
    GCAGATCAGG TGGTGTTTGT CAATCTCAGC AATGATGACG CTAATATCGA GTACGAGTAC
    GACTACGATG AGCAGAATCG CAAAATGGTG CTGGGCAAAG GTACTTATGG CACCGTGTAC
    GCCGCCAGGG ACAAGCAGAC GCAGGTGCGA ATCGCCATTA AGGAGGTGCC GGAAAAGAAC
    TCGCAGGATG TGCAGCCACT GCACGAGGAG ATTAAGTTAC ACTCTCAGCT GCGACATCGC
    AACATAGTTC GCTATTTGGG ATCGTGCTCG GAGAACGGCT TCTTCAAGAT CTTCATGGAG
    CAGGTGCCGG GAGGATCGCT TTCCGACCTC TTAGAGACCA AATGGGGTCC GCTCAAGGAC
    AACGAGTCCA CCATGGCCTT CTACTCCAAG CAGATCCTCG AGGGGCTGAA GTACCTTCAC
    GAGCAGGACA TTGTTCATCG CGATATAAAG GGTGACAACG TGCTGGTTAA CACGTATAGC
    GGCGTGGTTA AGATCTCCGA TTTCGGCACC TCCAAGCGGC TGGCCCGCAT CAACCCGATG
    ACGGAGACCT TTACGGGCAC CTTGCAGTAC ATGGCGCCGG AGGTGATTGA CCAGGGAGTA
    CGCGGCTATG GCCCGGCGGC TGACATCTGG TCCTTTGGAT GCACAAACGT TGAGATGGCT
    ACGGGCAAAC CACCGTTTAT CGAGCTGGGT AGCGCCCATG CGGCCATGTT CAAGGTGGGC
    TTTTACAAGA AGCATCCGAA CATACCCGAG GAGCTGTCCG CCAACGCAAA GAACTTTATT
    TTGCGTTGCT TTGCCATCAG CGTGATGGAC CGACCCTCCG CCTCGCAACT TCTGGAAGAT
    CCCTTCCTAC AAGATAAACC CCGCAAGGTG CGCCCAGCCC TGCCCATCAA TACTGAGTTC
    GGACGCAGCA TTTCGGTGCC GGCTGATCGG TTTGTGCACA AAACCACACC GCCGCTGAGC
    TACAACACAA CCTGCAATAC GCCCACAACG CCGGAATTGG ACATAACGCA TTCATCCTCG
    GTGGACATTG ACGAGCTGCC CAGCAACCAG TTTATGCTTG AACGGCGAAA TTCCCATGGA
    TTTTTGCTCT CGCCAGAGAT TGAACCTTCC ACGCCGTCCT TGCGTACGAG CATTAGTGAG
    ACGAGCGAGA CTGACGGTTT TTACAGGCTT AAAAAGGATT CGCAGAGGCG GACAACGCTT
    TCCAAGGTGC TGGCACTAGA CGAATCCAAG ATCTGCGATA TTTGGCTGAA GAAGGTCGAC
    GCTGATCAGA ACTCGATTGC GATTAGGAAA AGCGATTTGG AGGTTCTAAT CCGAGGACTG
    CGGGACTACA TTATGAACGA AAACGAAAAG CATTTGGAAG CGACTATCAA CGAACTGAAA
    CAAAAGCTGA ACAACGACGC AGTTGCTCTG GACCATCTTC ATTTGGCGCT GTACTCCTTT
    CAGGACGCCG TGGTCTGCGT ACTGCGGTAC CATTGCATCA AACCCCATTG GATGTTCGCA
    TTGGATAATC TGGTTAAGCG GGCCGTCCAG GCGGCGGTCA CTATTTTCTC TCCTGAACTG
    GGCGCCAATC TGGCGGACAA AGACCTGTCT GGAAATGATG ATGAGAGCTT GCGTACAAGT
    CTAATGCAGA AGGGTTCCAC CGAGAGCCAG GAAAAGCAGA GCCTGGAAAA AGTTCTTCCC
    ACGAGCAACA CTCGCGAAGG TGTGGGATTA ACGCCTGCGG AATCGGGCGG GGATGCTCTG
    TCCAGTTCGA TGGAGTGCGC GCGCATCCTG AGAGACATCC GTGACAATCA GCAGAAGCTG
    CAACGCCAGC TCCTTGAGCA GCAGCGGCAG AGCATGAGGG CCCTGCATAA TATCAGTCAG
    GAGCTGGCTC ACATTTACAT GGGCGGCAGA AAGAGATTAA GACGCACAAT AAACGGCTTT
    AACAAAAAGT GCCATAGAAT GAGTACCAGT GTGGGCGGAA CGTCCGGAGT CCGTCCGAAT
    ATAGCGGGCA ATCCCCGGAG GCAGCTAAAC AAGCATCACT TTGGTCTTCA CGAAGTAGAC
    GAGCAGTTGG AGCAGTGGTT GACCCAGCAG GAAATAGACG AGTTCTCAAA GACGCTCATC
    CTGAATGAAG GTTTTACATT TGACGACTTT ATCTATAACA TGGAAAAGCT GGACCTCATG
    AGGCTGGGTC TGCGCGTTGG CATCGAAGTG CGTTTGTGGA AACTCATTAT ACAAGAACGT
    GTGTGCACCT CGTCCGATTG TGTGGACAGT CCACCAAGCA CTTACCACAA ACCCATTGGA
    GGCAATAGCT CTTTTGCGCT TATTGACAAC AACAATACGC TACCATCACC CACAACCACA
    ACTCCCGGAA CGCCGGGCAT CAAAACGAAA ATCAAGAAAA GCGAAAGCGA GTACGATTCG
    TGCAGCGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.