subdued cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	subdued
Entrez Gene ID	42340
Full Name	CG16718 gene product from transcript CG16718-RA
Synonyms	CG16718,Dmel\CG16718
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 92A11-92A11

mRNA and Protein(s)

mRNA	Protein	Name
NM_142563.2	NP_650820.1	subdued, isoform A
NM_169867.3	NP_732439.1	subdued, isoform B
NM_001275807.1	NP_001262736.1	subdued, isoform E
NM_001202319.2	NP_001189248.1	subdued, isoform D
NM_001275808.1	NP_001262737.1	subdued, isoform F

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ANO1	XP_854489.4
Rattus norvegicus (Norway rat)	Ano1	NP_001101034.1
Pan troglodytes (chimpanzee)	ANO1	XP_003313240.1
Mus musculus (house mouse)	Ano1	NP_848757.4
Gallus gallus (chicken)	ANO1	XP_004941586.1
Homo sapiens (human)	ANO1	NP_060513.5
Danio rerio (zebrafish)	ano1	NP_001155062.1
	LOC708723	XP_002799504.1
Bos taurus (cattle)	ANO1	NP_001179646.1
Drosophila melanogaster (fruit fly)	CG16718	NP_001262736.1
Xenopus tropicalis (tropical clawed frog)	ano1	NP_001123799.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Subdued channels may amplify the nociceptive neuronal firing that is initiated by thermo-TRP channels in response to thermal stimuli
Title: The anoctamin family channel subdued mediates thermal nociception in Drosophila.

The following subdued gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the subdued cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05426	NM_169867.3 Latest version!	Drosophila melanogaster subdued, transcript variant B (subdued), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa05426	NM_001275807.1 Latest version!	Drosophila melanogaster subdued, transcript variant E (subdued), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa07809	NM_001275808.1 Latest version!	Drosophila melanogaster subdued, transcript variant F (subdued), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa05425	NM_142563.2 Latest version!	Drosophila melanogaster subdued, transcript variant A (subdued), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa07808	NM_001202319.2 Latest version!	Drosophila melanogaster subdued, transcript variant D (subdued), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

subdued ( NM_169867.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732439.1 Drosophila melanogaster subdued, transcript variant B (subdued), mRNA.

Price & Availability↑

CloneID	OFa05426
Clone ID Related Accession (Same CDS sequence)	NM_169867.3 , NM_001275807.1
Accession Version	NM_169867.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	subdued, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169867.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTGATC CGGATTTTGT GAAGGGCAAT GTAGGCGGCG TCTACGATGA TCGCGAGTGC 
AGCTGCCCCA GGGAGTTCTA CCAGCGGGGC GAGATTAACA CATCACTGTT CTTTGAGGAC 
TGCGTCCGGT CCATTGACTT TGTCCTGGCC TACAGGATCA ATGCGCACGA GCCCACGGAG 
CTGGAGAACA CGGAAAAGCG TCGTGTTTTC GAGGCGAATC TCATTAGTCA GGGGCTGGAG 
GTCGAGTCCA GTCAGAAGGA CCAGATCTGG TTCGTTAAGA TCCACGCTCC TTTGGAGGTC 
CTGCGACGGT ATGCGGAAAT TCTTAAGCTG CGCATGCCCA TGAAGGAGAT TCCAGGAATG 
TCCGTGGTCA ATCGATCGAC CAAAAGCGTG TTTTCTAGTC TTAAACATGT TTTCCAATTC 
TTTCTGCGCA ACATTTATGT GGACGAGGAA ATTTTTCCAA AGCGAGCTCA TCGATTCACT 
GCCATTTACA GTCGCGATAA GGAGTATCTC TTTGACATCC GACAGGATTG TTTCTTTACC 
ACCGCCGTCC GTTCCCGCAT CGTTGAGTTC ATCCTAGATC GCCAGCGATT TCCTGCCAAG 
AATCAACATG ACATGGCCTT CGGTATTGAG CGACTGATTG CCGAGGGTGT CTACTCTGCT 
GCCTATCCAC TCCATGATGG TGAAATAACC GAGACGGGAA CCATGAGAGC TCTGCTGTAC 
AAACATTGGG CCTCTGTGCC CAAATGGTAT CGCTATCAGC CCTTGGATGA CATCAAGGAA 
TACTTCGGTG TCAAAATTGG CTTGTACTTC GCCTGGCTGG GCTACTACAC CTACATGTTG 
CTGCTGGCCT CAATAGTGGG TGTCATTTGT TTCCTATATT CATGGTTCTC CCTTAAAAAC 
TATGTGCCTG TGAAAGATAT TTGCCAGAGC GGTAATACAA ACATAACCAT GTGTCCTCTT 
TGTGATTGGT GCAACTTTTG GGACCTGAAA GAGACCTGCA ACTATGCCAA AGTCACGTAC 
CTCATTGACA ATCCCAGCAC CGTGTTCTTC GCCGTTTTTA TGTCCTTTTG GGCCACCCTA 
TTTCTGGAGC TGTGGAAGCG GTACTCCGCC GAAATAACGC ATCGTTGGGA TCTGACGGGA 
TTCGATGTGC ATGAAGAGCA TCCGAGGCCA CAATATCTGG CGCGTTTGGA GCACATACCA 
CCGACGAGAG TGGATTATGT GACCAATATA AAAGAGCCAA CGGTTCCCTT TTGGCGTATG 
AAACTTCCCG CCACCGTGTT CAGCTTTTCA GTGGTACTGC TCCTAATTGC ACTGGCCTTT 
GTGGCACTGC TGGCAGTGGT TGTATACCGA ATGTCCATGC TGGCCGCCCT TAAAGTGGGT 
GCTAGTCCCA TGACCACCTC TAGCGCTATT GTCCTAGCCA CTGCATCAGC TGCCTTTGTA 
AATCTGTGCC TGCTCTATAT ACTTAATTAT ATGTACAATC ATTTGGCTGA GTACCTGACA 
GAGCTGGAAA TGTGGCGCAC TCAAACTCAG TTCGATGACT CGCTTACCCT TAAAATTTAT 
CTGCTGCAGT TTGTAAACTA CTACGCCTCC ATTTTTTACA TAGCTTTCTT CAAGGGTAAA 
TTCGTTGGTC ATCCGGGAGA GTATAATAAG CTTTTTGACT ATCGGCAGGA GGAGTGCTCA 
TCGGGCGGCT GTTTGACGGA GCTGTGCATC CAGTTAGCCA TTATAATGGT TGGCAAGCAG 
GCATTCAACA CTATTCTTGA AGTGTATCTT CCCATGTTCT GGCGAAAGGT TTTGGCCATT 
CAGGTGGGAC TGTCGCGACT TTTCAACAAC ACCCCGAATC CAGACAAGGC GAAAGACGAA 
CGCTGGATGC GGGATTTCAA GCTACTGGAT TGGGGTGCCC GAGGTCTGTT TCCCGAGTAT 
TTGGAGATGG TCTTGCAGTA CGGCTTCGTA ACCATCTTTG TGGCCGCTTT TCCGCTGGCG 
CCATTCTTTG CCCTGCTAAA TAATATCCTG GAAATGCGAC TGGATGCAAA GAAACTATTG 
ACCCACCACA AGCGTCCAGT ATCACAGCGA GTTCGAGATA TAGGAGTGTG GTATCGTATC 
CTGGACTGCA TAGGCAAGCT CAGCGTGATC ACAAATGGAT TCATCATTGC CTTTACCTCT 
GACATGATAC CGCGGTTGGT GTACCGGCAT TACGTAAACA AGCAAGGCAC TCTGGATGGT 
TACCTCAACT TTACACTATC AGAGTTCAAA GTCATTGATT CGCCCACGTT GTATAGCTTA 
GCTGGCGATC TCTCCAACAT AACAACGTGC AGATATACGG ACTTTCGACT TCCACCATCG 
TCTCCAGAAA AATACACATT GAGCAGCATG TTCTACATAA TTCTGGCCTG CCGATTGGGT 
TTTGTAGTCG TCTTTGAAAA CTTTGTTGCC CTGGTGATGA TTTTGGTGCG TTGGTGCATT 
CCGGACATGA GCGTTGAACT GCGTGACCAA ATCCGACGCG AAGTTTATGT GACCAATGAA 
ATTATCATCG ATCAAGAGGC CCACAGGGCT CGTTTTGAGC GTGCCAAGCG CAGCAGTTCG 
GTGCTCCAGA CGCCAGTTGA CCAGGACATG GATGCTGCCT CCATTCAAGA GCCTAATGCC 
AAGTTCATTA ACATTGAAAA GCTCCTTACT GAAAATCTCA CACAGATCGA AATGGATGCT 
ATTATACACG GCGAAAATCC AATCACAGGA ATTTCCGGTG CCGATTGCTT AGACGAAGTG 
CACAAAAAAC TCAGTGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732439.1
CDS	240..3020
Translation	MTDPDFVKGNVGGVYDDRECSCPREFYQRGEINTSLFFEDCVRSIDFVLAYRINAHEPTELENTEKRRVFEANLISQGLEVESSQKDQIWFVKIHAPLEVLRRYAEILKLRMPMKEIPGMSVVNRSTKSVFSSLKHVFQFFLRNIYVDEEIFPKRAHRFTAIYSRDKEYLFDIRQDCFFTTAVRSRIVEFILDRQRFPAKNQHDMAFGIERLIAEGVYSAAYPLHDGEITETGTMRALLYKHWASVPKWYRYQPLDDIKEYFGVKIGLYFAWLGYYTYMLLLASIVGVICFLYSWFSLKNYVPVKDICQSGNTNITMCPLCDWCNFWDLKETCNYAKVTYLIDNPSTVFFAVFMSFWATLFLELWKRYSAEITHRWDLTGFDVHEEHPRPQYLARLEHIPPTRVDYVTNIKEPTVPFWRMKLPATVFSFSVVLLLIALAFVALLAVVVYRMSMLAALKVGASPMTTSSAIVLATASAAFVNLCLLYILNYMYNHLAEYLTELEMWRTQTQFDDSLTLKIYLLQFVNYYASIFYIAFFKGKFVGHPGEYNKLFDYRQEECSSGGCLTELCIQLAIIMVGKQAFNTILEVYLPMFWRKVLAIQVGLSRLFNNTPNPDKAKDERWMRDFKLLDWGARGLFPEYLEMVLQYGFVTIFVAAFPLAPFFALLNNILEMRLDAKKLLTHHKRPVSQRVRDIGVWYRILDCIGKLSVITNGFIIAFTSDMIPRLVYRHYVNKQGTLDGYLNFTLSEFKVIDSPTLYSLAGDLSNITTCRYTDFRLPPSSPEKYTLSSMFYIILACRLGFVVVFENFVALVMILVRWCIPDMSVELRDQIRREVYVTNEIIIDQEAHRARFERAKRSSSVLQTPVDQDMDAASIQEPNAKFINIEKLLTENLTQIEMDAIIHGENPITGISGADCLDEVHKKLSD

Target ORF information:

RefSeq Version	NM_169867.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster subdued, transcript variant B (subdued), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169867.3

ORF Insert Sequence:

ATGACTGATC CGGATTTTGT GAAGGGCAAT GTAGGCGGCG TCTACGATGA TCGCGAGTGC 
AGCTGCCCCA GGGAGTTCTA CCAGCGGGGC GAGATTAACA CATCACTGTT CTTTGAGGAC 
TGCGTCCGGT CCATTGACTT TGTCCTGGCC TACAGGATCA ATGCGCACGA GCCCACGGAG 
CTGGAGAACA CGGAAAAGCG TCGTGTTTTC GAGGCGAATC TCATTAGTCA GGGGCTGGAG 
GTCGAGTCCA GTCAGAAGGA CCAGATCTGG TTCGTTAAGA TCCACGCTCC TTTGGAGGTC 
CTGCGACGGT ATGCGGAAAT TCTTAAGCTG CGCATGCCCA TGAAGGAGAT TCCAGGAATG 
TCCGTGGTCA ATCGATCGAC CAAAAGCGTG TTTTCTAGTC TTAAACATGT TTTCCAATTC 
TTTCTGCGCA ACATTTATGT GGACGAGGAA ATTTTTCCAA AGCGAGCTCA TCGATTCACT 
GCCATTTACA GTCGCGATAA GGAGTATCTC TTTGACATCC GACAGGATTG TTTCTTTACC 
ACCGCCGTCC GTTCCCGCAT CGTTGAGTTC ATCCTAGATC GCCAGCGATT TCCTGCCAAG 
AATCAACATG ACATGGCCTT CGGTATTGAG CGACTGATTG CCGAGGGTGT CTACTCTGCT 
GCCTATCCAC TCCATGATGG TGAAATAACC GAGACGGGAA CCATGAGAGC TCTGCTGTAC 
AAACATTGGG CCTCTGTGCC CAAATGGTAT CGCTATCAGC CCTTGGATGA CATCAAGGAA 
TACTTCGGTG TCAAAATTGG CTTGTACTTC GCCTGGCTGG GCTACTACAC CTACATGTTG 
CTGCTGGCCT CAATAGTGGG TGTCATTTGT TTCCTATATT CATGGTTCTC CCTTAAAAAC 
TATGTGCCTG TGAAAGATAT TTGCCAGAGC GGTAATACAA ACATAACCAT GTGTCCTCTT 
TGTGATTGGT GCAACTTTTG GGACCTGAAA GAGACCTGCA ACTATGCCAA AGTCACGTAC 
CTCATTGACA ATCCCAGCAC CGTGTTCTTC GCCGTTTTTA TGTCCTTTTG GGCCACCCTA 
TTTCTGGAGC TGTGGAAGCG GTACTCCGCC GAAATAACGC ATCGTTGGGA TCTGACGGGA 
TTCGATGTGC ATGAAGAGCA TCCGAGGCCA CAATATCTGG CGCGTTTGGA GCACATACCA 
CCGACGAGAG TGGATTATGT GACCAATATA AAAGAGCCAA CGGTTCCCTT TTGGCGTATG 
AAACTTCCCG CCACCGTGTT CAGCTTTTCA GTGGTACTGC TCCTAATTGC ACTGGCCTTT 
GTGGCACTGC TGGCAGTGGT TGTATACCGA ATGTCCATGC TGGCCGCCCT TAAAGTGGGT 
GCTAGTCCCA TGACCACCTC TAGCGCTATT GTCCTAGCCA CTGCATCAGC TGCCTTTGTA 
AATCTGTGCC TGCTCTATAT ACTTAATTAT ATGTACAATC ATTTGGCTGA GTACCTGACA 
GAGCTGGAAA TGTGGCGCAC TCAAACTCAG TTCGATGACT CGCTTACCCT TAAAATTTAT 
CTGCTGCAGT TTGTAAACTA CTACGCCTCC ATTTTTTACA TAGCTTTCTT CAAGGGTAAA 
TTCGTTGGTC ATCCGGGAGA GTATAATAAG CTTTTTGACT ATCGGCAGGA GGAGTGCTCA 
TCGGGCGGCT GTTTGACGGA GCTGTGCATC CAGTTAGCCA TTATAATGGT TGGCAAGCAG 
GCATTCAACA CTATTCTTGA AGTGTATCTT CCCATGTTCT GGCGAAAGGT TTTGGCCATT 
CAGGTGGGAC TGTCGCGACT TTTCAACAAC ACCCCGAATC CAGACAAGGC GAAAGACGAA 
CGCTGGATGC GGGATTTCAA GCTACTGGAT TGGGGTGCCC GAGGTCTGTT TCCCGAGTAT 
TTGGAGATGG TCTTGCAGTA CGGCTTCGTA ACCATCTTTG TGGCCGCTTT TCCGCTGGCG 
CCATTCTTTG CCCTGCTAAA TAATATCCTG GAAATGCGAC TGGATGCAAA GAAACTATTG 
ACCCACCACA AGCGTCCAGT ATCACAGCGA GTTCGAGATA TAGGAGTGTG GTATCGTATC 
CTGGACTGCA TAGGCAAGCT CAGCGTGATC ACAAATGGAT TCATCATTGC CTTTACCTCT 
GACATGATAC CGCGGTTGGT GTACCGGCAT TACGTAAACA AGCAAGGCAC TCTGGATGGT 
TACCTCAACT TTACACTATC AGAGTTCAAA GTCATTGATT CGCCCACGTT GTATAGCTTA 
GCTGGCGATC TCTCCAACAT AACAACGTGC AGATATACGG ACTTTCGACT TCCACCATCG 
TCTCCAGAAA AATACACATT GAGCAGCATG TTCTACATAA TTCTGGCCTG CCGATTGGGT 
TTTGTAGTCG TCTTTGAAAA CTTTGTTGCC CTGGTGATGA TTTTGGTGCG TTGGTGCATT 
CCGGACATGA GCGTTGAACT GCGTGACCAA ATCCGACGCG AAGTTTATGT GACCAATGAA 
ATTATCATCG ATCAAGAGGC CCACAGGGCT CGTTTTGAGC GTGCCAAGCG CAGCAGTTCG 
GTGCTCCAGA CGCCAGTTGA CCAGGACATG GATGCTGCCT CCATTCAAGA GCCTAATGCC 
AAGTTCATTA ACATTGAAAA GCTCCTTACT GAAAATCTCA CACAGATCGA AATGGATGCT 
ATTATACACG GCGAAAATCC AATCACAGGA ATTTCCGGTG CCGATTGCTT AGACGAAGTG 
CACAAAAAAC TCAGTGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

subdued ( NM_001275807.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262736.1 Drosophila melanogaster subdued, transcript variant E (subdued), mRNA.

Price & Availability↑

CloneID	OFa05426
Clone ID Related Accession (Same CDS sequence)	NM_169867.3 , NM_001275807.1
Accession Version	NM_001275807.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	subdued, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTGATC CGGATTTTGT GAAGGGCAAT GTAGGCGGCG TCTACGATGA TCGCGAGTGC 
AGCTGCCCCA GGGAGTTCTA CCAGCGGGGC GAGATTAACA CATCACTGTT CTTTGAGGAC 
TGCGTCCGGT CCATTGACTT TGTCCTGGCC TACAGGATCA ATGCGCACGA GCCCACGGAG 
CTGGAGAACA CGGAAAAGCG TCGTGTTTTC GAGGCGAATC TCATTAGTCA GGGGCTGGAG 
GTCGAGTCCA GTCAGAAGGA CCAGATCTGG TTCGTTAAGA TCCACGCTCC TTTGGAGGTC 
CTGCGACGGT ATGCGGAAAT TCTTAAGCTG CGCATGCCCA TGAAGGAGAT TCCAGGAATG 
TCCGTGGTCA ATCGATCGAC CAAAAGCGTG TTTTCTAGTC TTAAACATGT TTTCCAATTC 
TTTCTGCGCA ACATTTATGT GGACGAGGAA ATTTTTCCAA AGCGAGCTCA TCGATTCACT 
GCCATTTACA GTCGCGATAA GGAGTATCTC TTTGACATCC GACAGGATTG TTTCTTTACC 
ACCGCCGTCC GTTCCCGCAT CGTTGAGTTC ATCCTAGATC GCCAGCGATT TCCTGCCAAG 
AATCAACATG ACATGGCCTT CGGTATTGAG CGACTGATTG CCGAGGGTGT CTACTCTGCT 
GCCTATCCAC TCCATGATGG TGAAATAACC GAGACGGGAA CCATGAGAGC TCTGCTGTAC 
AAACATTGGG CCTCTGTGCC CAAATGGTAT CGCTATCAGC CCTTGGATGA CATCAAGGAA 
TACTTCGGTG TCAAAATTGG CTTGTACTTC GCCTGGCTGG GCTACTACAC CTACATGTTG 
CTGCTGGCCT CAATAGTGGG TGTCATTTGT TTCCTATATT CATGGTTCTC CCTTAAAAAC 
TATGTGCCTG TGAAAGATAT TTGCCAGAGC GGTAATACAA ACATAACCAT GTGTCCTCTT 
TGTGATTGGT GCAACTTTTG GGACCTGAAA GAGACCTGCA ACTATGCCAA AGTCACGTAC 
CTCATTGACA ATCCCAGCAC CGTGTTCTTC GCCGTTTTTA TGTCCTTTTG GGCCACCCTA 
TTTCTGGAGC TGTGGAAGCG GTACTCCGCC GAAATAACGC ATCGTTGGGA TCTGACGGGA 
TTCGATGTGC ATGAAGAGCA TCCGAGGCCA CAATATCTGG CGCGTTTGGA GCACATACCA 
CCGACGAGAG TGGATTATGT GACCAATATA AAAGAGCCAA CGGTTCCCTT TTGGCGTATG 
AAACTTCCCG CCACCGTGTT CAGCTTTTCA GTGGTACTGC TCCTAATTGC ACTGGCCTTT 
GTGGCACTGC TGGCAGTGGT TGTATACCGA ATGTCCATGC TGGCCGCCCT TAAAGTGGGT 
GCTAGTCCCA TGACCACCTC TAGCGCTATT GTCCTAGCCA CTGCATCAGC TGCCTTTGTA 
AATCTGTGCC TGCTCTATAT ACTTAATTAT ATGTACAATC ATTTGGCTGA GTACCTGACA 
GAGCTGGAAA TGTGGCGCAC TCAAACTCAG TTCGATGACT CGCTTACCCT TAAAATTTAT 
CTGCTGCAGT TTGTAAACTA CTACGCCTCC ATTTTTTACA TAGCTTTCTT CAAGGGTAAA 
TTCGTTGGTC ATCCGGGAGA GTATAATAAG CTTTTTGACT ATCGGCAGGA GGAGTGCTCA 
TCGGGCGGCT GTTTGACGGA GCTGTGCATC CAGTTAGCCA TTATAATGGT TGGCAAGCAG 
GCATTCAACA CTATTCTTGA AGTGTATCTT CCCATGTTCT GGCGAAAGGT TTTGGCCATT 
CAGGTGGGAC TGTCGCGACT TTTCAACAAC ACCCCGAATC CAGACAAGGC GAAAGACGAA 
CGCTGGATGC GGGATTTCAA GCTACTGGAT TGGGGTGCCC GAGGTCTGTT TCCCGAGTAT 
TTGGAGATGG TCTTGCAGTA CGGCTTCGTA ACCATCTTTG TGGCCGCTTT TCCGCTGGCG 
CCATTCTTTG CCCTGCTAAA TAATATCCTG GAAATGCGAC TGGATGCAAA GAAACTATTG 
ACCCACCACA AGCGTCCAGT ATCACAGCGA GTTCGAGATA TAGGAGTGTG GTATCGTATC 
CTGGACTGCA TAGGCAAGCT CAGCGTGATC ACAAATGGAT TCATCATTGC CTTTACCTCT 
GACATGATAC CGCGGTTGGT GTACCGGCAT TACGTAAACA AGCAAGGCAC TCTGGATGGT 
TACCTCAACT TTACACTATC AGAGTTCAAA GTCATTGATT CGCCCACGTT GTATAGCTTA 
GCTGGCGATC TCTCCAACAT AACAACGTGC AGATATACGG ACTTTCGACT TCCACCATCG 
TCTCCAGAAA AATACACATT GAGCAGCATG TTCTACATAA TTCTGGCCTG CCGATTGGGT 
TTTGTAGTCG TCTTTGAAAA CTTTGTTGCC CTGGTGATGA TTTTGGTGCG TTGGTGCATT 
CCGGACATGA GCGTTGAACT GCGTGACCAA ATCCGACGCG AAGTTTATGT GACCAATGAA 
ATTATCATCG ATCAAGAGGC CCACAGGGCT CGTTTTGAGC GTGCCAAGCG CAGCAGTTCG 
GTGCTCCAGA CGCCAGTTGA CCAGGACATG GATGCTGCCT CCATTCAAGA GCCTAATGCC 
AAGTTCATTA ACATTGAAAA GCTCCTTACT GAAAATCTCA CACAGATCGA AATGGATGCT 
ATTATACACG GCGAAAATCC AATCACAGGA ATTTCCGGTG CCGATTGCTT AGACGAAGTG 
CACAAAAAAC TCAGTGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262736.1
CDS	213..2993
Translation	MTDPDFVKGNVGGVYDDRECSCPREFYQRGEINTSLFFEDCVRSIDFVLAYRINAHEPTELENTEKRRVFEANLISQGLEVESSQKDQIWFVKIHAPLEVLRRYAEILKLRMPMKEIPGMSVVNRSTKSVFSSLKHVFQFFLRNIYVDEEIFPKRAHRFTAIYSRDKEYLFDIRQDCFFTTAVRSRIVEFILDRQRFPAKNQHDMAFGIERLIAEGVYSAAYPLHDGEITETGTMRALLYKHWASVPKWYRYQPLDDIKEYFGVKIGLYFAWLGYYTYMLLLASIVGVICFLYSWFSLKNYVPVKDICQSGNTNITMCPLCDWCNFWDLKETCNYAKVTYLIDNPSTVFFAVFMSFWATLFLELWKRYSAEITHRWDLTGFDVHEEHPRPQYLARLEHIPPTRVDYVTNIKEPTVPFWRMKLPATVFSFSVVLLLIALAFVALLAVVVYRMSMLAALKVGASPMTTSSAIVLATASAAFVNLCLLYILNYMYNHLAEYLTELEMWRTQTQFDDSLTLKIYLLQFVNYYASIFYIAFFKGKFVGHPGEYNKLFDYRQEECSSGGCLTELCIQLAIIMVGKQAFNTILEVYLPMFWRKVLAIQVGLSRLFNNTPNPDKAKDERWMRDFKLLDWGARGLFPEYLEMVLQYGFVTIFVAAFPLAPFFALLNNILEMRLDAKKLLTHHKRPVSQRVRDIGVWYRILDCIGKLSVITNGFIIAFTSDMIPRLVYRHYVNKQGTLDGYLNFTLSEFKVIDSPTLYSLAGDLSNITTCRYTDFRLPPSSPEKYTLSSMFYIILACRLGFVVVFENFVALVMILVRWCIPDMSVELRDQIRREVYVTNEIIIDQEAHRARFERAKRSSSVLQTPVDQDMDAASIQEPNAKFINIEKLLTENLTQIEMDAIIHGENPITGISGADCLDEVHKKLSD

Target ORF information:

RefSeq Version	NM_001275807.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster subdued, transcript variant E (subdued), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275807.1

ORF Insert Sequence:

ATGACTGATC CGGATTTTGT GAAGGGCAAT GTAGGCGGCG TCTACGATGA TCGCGAGTGC 
AGCTGCCCCA GGGAGTTCTA CCAGCGGGGC GAGATTAACA CATCACTGTT CTTTGAGGAC 
TGCGTCCGGT CCATTGACTT TGTCCTGGCC TACAGGATCA ATGCGCACGA GCCCACGGAG 
CTGGAGAACA CGGAAAAGCG TCGTGTTTTC GAGGCGAATC TCATTAGTCA GGGGCTGGAG 
GTCGAGTCCA GTCAGAAGGA CCAGATCTGG TTCGTTAAGA TCCACGCTCC TTTGGAGGTC 
CTGCGACGGT ATGCGGAAAT TCTTAAGCTG CGCATGCCCA TGAAGGAGAT TCCAGGAATG 
TCCGTGGTCA ATCGATCGAC CAAAAGCGTG TTTTCTAGTC TTAAACATGT TTTCCAATTC 
TTTCTGCGCA ACATTTATGT GGACGAGGAA ATTTTTCCAA AGCGAGCTCA TCGATTCACT 
GCCATTTACA GTCGCGATAA GGAGTATCTC TTTGACATCC GACAGGATTG TTTCTTTACC 
ACCGCCGTCC GTTCCCGCAT CGTTGAGTTC ATCCTAGATC GCCAGCGATT TCCTGCCAAG 
AATCAACATG ACATGGCCTT CGGTATTGAG CGACTGATTG CCGAGGGTGT CTACTCTGCT 
GCCTATCCAC TCCATGATGG TGAAATAACC GAGACGGGAA CCATGAGAGC TCTGCTGTAC 
AAACATTGGG CCTCTGTGCC CAAATGGTAT CGCTATCAGC CCTTGGATGA CATCAAGGAA 
TACTTCGGTG TCAAAATTGG CTTGTACTTC GCCTGGCTGG GCTACTACAC CTACATGTTG 
CTGCTGGCCT CAATAGTGGG TGTCATTTGT TTCCTATATT CATGGTTCTC CCTTAAAAAC 
TATGTGCCTG TGAAAGATAT TTGCCAGAGC GGTAATACAA ACATAACCAT GTGTCCTCTT 
TGTGATTGGT GCAACTTTTG GGACCTGAAA GAGACCTGCA ACTATGCCAA AGTCACGTAC 
CTCATTGACA ATCCCAGCAC CGTGTTCTTC GCCGTTTTTA TGTCCTTTTG GGCCACCCTA 
TTTCTGGAGC TGTGGAAGCG GTACTCCGCC GAAATAACGC ATCGTTGGGA TCTGACGGGA 
TTCGATGTGC ATGAAGAGCA TCCGAGGCCA CAATATCTGG CGCGTTTGGA GCACATACCA 
CCGACGAGAG TGGATTATGT GACCAATATA AAAGAGCCAA CGGTTCCCTT TTGGCGTATG 
AAACTTCCCG CCACCGTGTT CAGCTTTTCA GTGGTACTGC TCCTAATTGC ACTGGCCTTT 
GTGGCACTGC TGGCAGTGGT TGTATACCGA ATGTCCATGC TGGCCGCCCT TAAAGTGGGT 
GCTAGTCCCA TGACCACCTC TAGCGCTATT GTCCTAGCCA CTGCATCAGC TGCCTTTGTA 
AATCTGTGCC TGCTCTATAT ACTTAATTAT ATGTACAATC ATTTGGCTGA GTACCTGACA 
GAGCTGGAAA TGTGGCGCAC TCAAACTCAG TTCGATGACT CGCTTACCCT TAAAATTTAT 
CTGCTGCAGT TTGTAAACTA CTACGCCTCC ATTTTTTACA TAGCTTTCTT CAAGGGTAAA 
TTCGTTGGTC ATCCGGGAGA GTATAATAAG CTTTTTGACT ATCGGCAGGA GGAGTGCTCA 
TCGGGCGGCT GTTTGACGGA GCTGTGCATC CAGTTAGCCA TTATAATGGT TGGCAAGCAG 
GCATTCAACA CTATTCTTGA AGTGTATCTT CCCATGTTCT GGCGAAAGGT TTTGGCCATT 
CAGGTGGGAC TGTCGCGACT TTTCAACAAC ACCCCGAATC CAGACAAGGC GAAAGACGAA 
CGCTGGATGC GGGATTTCAA GCTACTGGAT TGGGGTGCCC GAGGTCTGTT TCCCGAGTAT 
TTGGAGATGG TCTTGCAGTA CGGCTTCGTA ACCATCTTTG TGGCCGCTTT TCCGCTGGCG 
CCATTCTTTG CCCTGCTAAA TAATATCCTG GAAATGCGAC TGGATGCAAA GAAACTATTG 
ACCCACCACA AGCGTCCAGT ATCACAGCGA GTTCGAGATA TAGGAGTGTG GTATCGTATC 
CTGGACTGCA TAGGCAAGCT CAGCGTGATC ACAAATGGAT TCATCATTGC CTTTACCTCT 
GACATGATAC CGCGGTTGGT GTACCGGCAT TACGTAAACA AGCAAGGCAC TCTGGATGGT 
TACCTCAACT TTACACTATC AGAGTTCAAA GTCATTGATT CGCCCACGTT GTATAGCTTA 
GCTGGCGATC TCTCCAACAT AACAACGTGC AGATATACGG ACTTTCGACT TCCACCATCG 
TCTCCAGAAA AATACACATT GAGCAGCATG TTCTACATAA TTCTGGCCTG CCGATTGGGT 
TTTGTAGTCG TCTTTGAAAA CTTTGTTGCC CTGGTGATGA TTTTGGTGCG TTGGTGCATT 
CCGGACATGA GCGTTGAACT GCGTGACCAA ATCCGACGCG AAGTTTATGT GACCAATGAA 
ATTATCATCG ATCAAGAGGC CCACAGGGCT CGTTTTGAGC GTGCCAAGCG CAGCAGTTCG 
GTGCTCCAGA CGCCAGTTGA CCAGGACATG GATGCTGCCT CCATTCAAGA GCCTAATGCC 
AAGTTCATTA ACATTGAAAA GCTCCTTACT GAAAATCTCA CACAGATCGA AATGGATGCT 
ATTATACACG GCGAAAATCC AATCACAGGA ATTTCCGGTG CCGATTGCTT AGACGAAGTG 
CACAAAAAAC TCAGTGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

subdued ( NM_001275808.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262737.1 Drosophila melanogaster subdued, transcript variant F (subdued), mRNA.

Price & Availability↑

CloneID	OFa07809
Clone ID Related Accession (Same CDS sequence)	NM_001275808.1
Accession Version	NM_001275808.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	subdued, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTGATC CGGATTTTGT GAAGGGCAAT GTAGGCGGCG TCTACGATGA TCGCGAGTGC 
AGCTGCCCCA GGGAGTTCTA CCAGCGGGGC GAGATTAACA CATCACTGTT CTTTGAGGAC 
TGCGTCCGGT CCATTGACTT TGTCCTGGCC TACAGGATCA ATGCGCACGA GCCCACGGAG 
CTGGAGAACA CGGAAAAGCG TCGTGTTTTC GAGGCGAATC TCATTAGTCA GGGGCTGGAG 
GTCGAGTCCA GTCAGAAGGA CCAGATCTGG TTCGTTAAGA TCCACGCTCC TTTGGAGGTC 
CTGCGACGGT ATGCGGAAAT TCTTAAGCTG CGCATGCCCA TGAAGGAGTC GTTATGCAAT 
TTACGCATAT CTGAAAGATC GAATCGCCTA CGAAATGCGG CCCATTACTT GACCAATAAG 
ATTCCAGGAA TGTCCGTGGT CAATCGATCG ACCAAAAGCG TGTTTTCTAG TCTTAAACAT 
GTTTTCCAAT TCTTTCTGCG CAACATTTAT GTGGACGAGG AAATTTTTCC AAAGCGAGCT 
CATCGATTCA CTGCCATTTA CAGTCGCGAT AAGGAGTATC TCTTTGACAT CCGACAGGAT 
TGTTTCTTTA CCACCGCCGT CCGTTCCCGC ATCGTTGAGT TCATCCTAGA TCGCCAGCGA 
TTTCCTGCCA AGAATCAACA TGACATGGCC TTCGGTATTG AGCGACTGAT TGCCGAGGGT 
GTCTACTCTG CTGCCTATCC ACTCCATGAT GGTGAAATAA CCGAGACGGG AACCATGAGA 
GCTCTGCTGT ACAAACATTG GGCCTCTGTG CCCAAATGGT ATCGCTATCA GCCCTTGGAT 
GACATCAAGG AATACTTCGG TGTCAAAATT GGCTTGTACT TCGCCTGGCT GGGCTACTAC 
ACCTACATGT TGCTGCTGGC CTCAATAGTG GGTGTCATTT GTTTCCTATA TTCATGGTTC 
TCCCTTAAAA ACTATGTGCC TGTGAAAGAT ATTTGCCAGA GCGGTAATAC AAACATAACC 
ATGTGTCCTC TTTGTGATTG GTGCAACTTT TGGGACCTGA AAGAGACCTG CAACTATGCC 
AAAGTCACGT ACCTCATTGA CAATCCCAGC ACCGTGTTCT TCGCCGTTTT TATGTCCTTT 
TGGGCCACCC TATTTCTGGA GCTGTGGAAG CGGTACTCCG CCGAAATAAC GCATCGTTGG 
GATCTGACGG GATTCGATGT GCATGAAGAG CATCCGAGGC CACAATATCT GGCGCGTTTG 
GAGCACATAC CACCGACGAG AGTGGATTAT GTGACCAATA TAAAAGAGCC AACGGTTCCC 
TTTTGGCGTA TGAAACTTCC CGCCACCGTG TTCAGCTTTT CAGTGGTACT GCTCCTAATT 
GCACTGGCCT TTGTGGCACT GCTGGCAGTG GTTGTATACC GAATGTCCAT GCTGGCCGCC 
CTTAAAGTGG GTGCTAGTCC CATGACCACC TCTAGCGCTA TTGTCCTAGC CACTGCATCA 
GCTGCCTTTG TAAATCTGTG CCTGCTCTAT ATACTTAATT ATATGTACAA TCATTTGGCT 
GAGTACCTGA CAGAGCTGGA AATGTGGCGC ACTCAAACTC AGTTCGATGA CTCGCTTACC 
CTTAAAATTT ATCTGCTGCA GTTTGTAAAC TACTACGCCT CCATTTTTTA CATAGCTTTC 
TTCAAGGGTA AATTCGTTGG TCATCCGGGA GAGTATAATA AGCTTTTTGA CTATCGGCAG 
GAGGAGTGCT CATCGGGCGG CTGTTTGACG GAGCTGTGCA TCCAGTTAGC CATTATAATG 
GTTGGCAAGC AGGCATTCAA CACTATTCTT GAAGTGTATC TTCCCATGTT CTGGCGAAAG 
GTTTTGGCCA TTCAGGTGGG ACTGTCGCGA CTTTTCAACA ACACCCCGAA TCCAGACAAG 
GCGAAAGACG AACGCTGGAT GCGGGATTTC AAGCTACTGG ATTGGGGTGC CCGAGGTCTG 
TTTCCCGAGT ATTTGGAGAT GGTCTTGCAG TACGGCTTCG TAACCATCTT TGTGGCCGCT 
TTTCCGCTGG CGCCATTCTT TGCCCTGCTA AATAATATCC TGGAAATGCG ACTGGATGCA 
AAGAAACTAT TGACCCACCA CAAGCGTCCA GTATCACAGC GAGTTCGAGA TATAGGAGTG 
TGGTATCGTA TCCTGGACTG CATAGGCAAG CTCAGCGTGA TCACAAATGG ATTCATCATT 
GCCTTTACCT CTGACATGAT ACCGCGGTTG GTGTACCGGC ATTACGTAAA CAAGCAAGGC 
ACTCTGGATG GTTACCTCAA CTTTACACTA TCAGAGTTCA AAGTCATTGA TTCGCCCACG 
TTGTATAGCT TAGCTGGCGA TCTCTCCAAC ATAACAACGT GCAGATATAC GGACTTTCGA 
CTTCCACCAT CGTCTCCAGA AAAATACACA TTGAGCAGCA TGTTCTACAT AATTCTGGCC 
TGCCGATTGG GTTTTGTAGT CGTCTTTGAA AACTTTGTTG CCCTGGTGAT GATTTTGGTG 
CGTTGGTGCA TTCCGGACAT GAGCGTTGAA CTGCGTGACC AAATCCGACG CGAAGTTTAT 
GTGACCAATG AAATTATCAT CGATCAAGAG GCCCACAGGG CTCGTTTTGA GCGTGCCAAG 
CGCAGCAGTT CGGTGCTCCA GACGCCAGTT GACCAGGACA TGGATGCTGC CTCCATTCAA 
GAGCCTAATG CCAAGTTCAT TAACATTGAA AAGCTCCTTA CTGAAAATCT CACACAGATC 
GAAATGGATG CTATTATACA CGGCGAAAAT CCAATCACAG GAATTTCCGG TGCCGATTGC 
TTAGACGAAG TGCACAAAAA ACTCAGTGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262737.1
CDS	240..3092
Translation	MTDPDFVKGNVGGVYDDRECSCPREFYQRGEINTSLFFEDCVRSIDFVLAYRINAHEPTELENTEKRRVFEANLISQGLEVESSQKDQIWFVKIHAPLEVLRRYAEILKLRMPMKESLCNLRISERSNRLRNAAHYLTNKIPGMSVVNRSTKSVFSSLKHVFQFFLRNIYVDEEIFPKRAHRFTAIYSRDKEYLFDIRQDCFFTTAVRSRIVEFILDRQRFPAKNQHDMAFGIERLIAEGVYSAAYPLHDGEITETGTMRALLYKHWASVPKWYRYQPLDDIKEYFGVKIGLYFAWLGYYTYMLLLASIVGVICFLYSWFSLKNYVPVKDICQSGNTNITMCPLCDWCNFWDLKETCNYAKVTYLIDNPSTVFFAVFMSFWATLFLELWKRYSAEITHRWDLTGFDVHEEHPRPQYLARLEHIPPTRVDYVTNIKEPTVPFWRMKLPATVFSFSVVLLLIALAFVALLAVVVYRMSMLAALKVGASPMTTSSAIVLATASAAFVNLCLLYILNYMYNHLAEYLTELEMWRTQTQFDDSLTLKIYLLQFVNYYASIFYIAFFKGKFVGHPGEYNKLFDYRQEECSSGGCLTELCIQLAIIMVGKQAFNTILEVYLPMFWRKVLAIQVGLSRLFNNTPNPDKAKDERWMRDFKLLDWGARGLFPEYLEMVLQYGFVTIFVAAFPLAPFFALLNNILEMRLDAKKLLTHHKRPVSQRVRDIGVWYRILDCIGKLSVITNGFIIAFTSDMIPRLVYRHYVNKQGTLDGYLNFTLSEFKVIDSPTLYSLAGDLSNITTCRYTDFRLPPSSPEKYTLSSMFYIILACRLGFVVVFENFVALVMILVRWCIPDMSVELRDQIRREVYVTNEIIIDQEAHRARFERAKRSSSVLQTPVDQDMDAASIQEPNAKFINIEKLLTENLTQIEMDAIIHGENPITGISGADCLDEVHKKLSD

Target ORF information:

RefSeq Version	NM_001275808.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster subdued, transcript variant F (subdued), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275808.1

ORF Insert Sequence:

ATGACTGATC CGGATTTTGT GAAGGGCAAT GTAGGCGGCG TCTACGATGA TCGCGAGTGC 
AGCTGCCCCA GGGAGTTCTA CCAGCGGGGC GAGATTAACA CATCACTGTT CTTTGAGGAC 
TGCGTCCGGT CCATTGACTT TGTCCTGGCC TACAGGATCA ATGCGCACGA GCCCACGGAG 
CTGGAGAACA CGGAAAAGCG TCGTGTTTTC GAGGCGAATC TCATTAGTCA GGGGCTGGAG 
GTCGAGTCCA GTCAGAAGGA CCAGATCTGG TTCGTTAAGA TCCACGCTCC TTTGGAGGTC 
CTGCGACGGT ATGCGGAAAT TCTTAAGCTG CGCATGCCCA TGAAGGAGTC GTTATGCAAT 
TTACGCATAT CTGAAAGATC GAATCGCCTA CGAAATGCGG CCCATTACTT GACCAATAAG 
ATTCCAGGAA TGTCCGTGGT CAATCGATCG ACCAAAAGCG TGTTTTCTAG TCTTAAACAT 
GTTTTCCAAT TCTTTCTGCG CAACATTTAT GTGGACGAGG AAATTTTTCC AAAGCGAGCT 
CATCGATTCA CTGCCATTTA CAGTCGCGAT AAGGAGTATC TCTTTGACAT CCGACAGGAT 
TGTTTCTTTA CCACCGCCGT CCGTTCCCGC ATCGTTGAGT TCATCCTAGA TCGCCAGCGA 
TTTCCTGCCA AGAATCAACA TGACATGGCC TTCGGTATTG AGCGACTGAT TGCCGAGGGT 
GTCTACTCTG CTGCCTATCC ACTCCATGAT GGTGAAATAA CCGAGACGGG AACCATGAGA 
GCTCTGCTGT ACAAACATTG GGCCTCTGTG CCCAAATGGT ATCGCTATCA GCCCTTGGAT 
GACATCAAGG AATACTTCGG TGTCAAAATT GGCTTGTACT TCGCCTGGCT GGGCTACTAC 
ACCTACATGT TGCTGCTGGC CTCAATAGTG GGTGTCATTT GTTTCCTATA TTCATGGTTC 
TCCCTTAAAA ACTATGTGCC TGTGAAAGAT ATTTGCCAGA GCGGTAATAC AAACATAACC 
ATGTGTCCTC TTTGTGATTG GTGCAACTTT TGGGACCTGA AAGAGACCTG CAACTATGCC 
AAAGTCACGT ACCTCATTGA CAATCCCAGC ACCGTGTTCT TCGCCGTTTT TATGTCCTTT 
TGGGCCACCC TATTTCTGGA GCTGTGGAAG CGGTACTCCG CCGAAATAAC GCATCGTTGG 
GATCTGACGG GATTCGATGT GCATGAAGAG CATCCGAGGC CACAATATCT GGCGCGTTTG 
GAGCACATAC CACCGACGAG AGTGGATTAT GTGACCAATA TAAAAGAGCC AACGGTTCCC 
TTTTGGCGTA TGAAACTTCC CGCCACCGTG TTCAGCTTTT CAGTGGTACT GCTCCTAATT 
GCACTGGCCT TTGTGGCACT GCTGGCAGTG GTTGTATACC GAATGTCCAT GCTGGCCGCC 
CTTAAAGTGG GTGCTAGTCC CATGACCACC TCTAGCGCTA TTGTCCTAGC CACTGCATCA 
GCTGCCTTTG TAAATCTGTG CCTGCTCTAT ATACTTAATT ATATGTACAA TCATTTGGCT 
GAGTACCTGA CAGAGCTGGA AATGTGGCGC ACTCAAACTC AGTTCGATGA CTCGCTTACC 
CTTAAAATTT ATCTGCTGCA GTTTGTAAAC TACTACGCCT CCATTTTTTA CATAGCTTTC 
TTCAAGGGTA AATTCGTTGG TCATCCGGGA GAGTATAATA AGCTTTTTGA CTATCGGCAG 
GAGGAGTGCT CATCGGGCGG CTGTTTGACG GAGCTGTGCA TCCAGTTAGC CATTATAATG 
GTTGGCAAGC AGGCATTCAA CACTATTCTT GAAGTGTATC TTCCCATGTT CTGGCGAAAG 
GTTTTGGCCA TTCAGGTGGG ACTGTCGCGA CTTTTCAACA ACACCCCGAA TCCAGACAAG 
GCGAAAGACG AACGCTGGAT GCGGGATTTC AAGCTACTGG ATTGGGGTGC CCGAGGTCTG 
TTTCCCGAGT ATTTGGAGAT GGTCTTGCAG TACGGCTTCG TAACCATCTT TGTGGCCGCT 
TTTCCGCTGG CGCCATTCTT TGCCCTGCTA AATAATATCC TGGAAATGCG ACTGGATGCA 
AAGAAACTAT TGACCCACCA CAAGCGTCCA GTATCACAGC GAGTTCGAGA TATAGGAGTG 
TGGTATCGTA TCCTGGACTG CATAGGCAAG CTCAGCGTGA TCACAAATGG ATTCATCATT 
GCCTTTACCT CTGACATGAT ACCGCGGTTG GTGTACCGGC ATTACGTAAA CAAGCAAGGC 
ACTCTGGATG GTTACCTCAA CTTTACACTA TCAGAGTTCA AAGTCATTGA TTCGCCCACG 
TTGTATAGCT TAGCTGGCGA TCTCTCCAAC ATAACAACGT GCAGATATAC GGACTTTCGA 
CTTCCACCAT CGTCTCCAGA AAAATACACA TTGAGCAGCA TGTTCTACAT AATTCTGGCC 
TGCCGATTGG GTTTTGTAGT CGTCTTTGAA AACTTTGTTG CCCTGGTGAT GATTTTGGTG 
CGTTGGTGCA TTCCGGACAT GAGCGTTGAA CTGCGTGACC AAATCCGACG CGAAGTTTAT 
GTGACCAATG AAATTATCAT CGATCAAGAG GCCCACAGGG CTCGTTTTGA GCGTGCCAAG 
CGCAGCAGTT CGGTGCTCCA GACGCCAGTT GACCAGGACA TGGATGCTGC CTCCATTCAA 
GAGCCTAATG CCAAGTTCAT TAACATTGAA AAGCTCCTTA CTGAAAATCT CACACAGATC 
GAAATGGATG CTATTATACA CGGCGAAAAT CCAATCACAG GAATTTCCGG TGCCGATTGC 
TTAGACGAAG TGCACAAAAA ACTCAGTGAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

subdued ( NM_142563.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650820.1 Drosophila melanogaster subdued, transcript variant A (subdued), mRNA.

Price & Availability↑

CloneID	OFa05425
Clone ID Related Accession (Same CDS sequence)	NM_142563.2
Accession Version	NM_142563.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	subdued, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142563.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTAAATT TAGGCAGTGC CAAAGTTGGC GCTCTGGAGG AGGCCACCTC ACCACCCCCC 
TTCTCCAAGG ACGAGAGCTT CCAGCTGTTG GAGCGGAGCC CCCACAATCC CGCCATATCA 
CCCACCACCA CTGTCCCGCT GGAGCCCCCC TTCACCACCC CCCTAGCTCC CTTCGACATG 
GAGCTGATGA AGCGCCACAA TCTCCACGAG CGCTTAACGG GAAAGGAGAG CTTCTGGCGC 
GGGCGCGACA CTTCGCAGAG ATTTCTGGAC CAGGAGCAAT TATCAGCATC GCGTGAATCA 
CTCGGTGCCG CCGACAAGAG AGCCCGGTTT GCAGAAAGCA ACGCCCCCAC GCTCCAACTG 
CCAGAATCTG CCGAGCCCAG GGCCAATGGC AAAAGACCCA CTGGGAACAG GACCAACCCA 
TCGAATCCAG CCGCTCCCCT TCTGCCAATG ACTGATCCGG ATTTTGTGAA GGGCAATGTA 
GGCGGCGTCT ACGATGATCG CGAGTGCAGC TGCCCCAGGG AGTTCTACCA GCGGGGCGAG 
ATTAACACAT CACTGTTCTT TGAGGACTGC GTCCGGTCCA TTGACTTTGT CCTGGCCTAC 
AGGATCAATG CGCACGAGCC CACGGAGCTG GAGAACACGG AAAAGCGTCG TGTTTTCGAG 
GCGAATCTCA TTAGTCAGGG GCTGGAGGTC GAGTCCAGTC AGAAGGACCA GATCTGGTTC 
GTTAAGATCC ACGCTCCTTT GGAGGTCCTG CGACGGTATG CGGAAATTCT TAAGCTGCGC 
ATGCCCATGA AGGAGATTCC AGGAATGTCC GTGGTCAATC GATCGACCAA AAGCGTGTTT 
TCTAGTCTTA AACATGTTTT CCAATTCTTT CTGCGCAACA TTTATGTGGA CGAGGAAATT 
TTTCCAAAGC GAGCTCATCG ATTCACTGCC ATTTACAGTC GCGATAAGGA GTATCTCTTT 
GACATCCGAC AGGATTGTTT CTTTACCACC GCCGTCCGTT CCCGCATCGT TGAGTTCATC 
CTAGATCGCC AGCGATTTCC TGCCAAGAAT CAACATGACA TGGCCTTCGG TATTGAGCGA 
CTGATTGCCG AGGGTGTCTA CTCTGCTGCC TATCCACTCC ATGATGGTGA AATAACCGAG 
ACGGGAACCA TGAGAGCTCT GCTGTACAAA CATTGGGCCT CTGTGCCCAA ATGGTATCGC 
TATCAGCCCT TGGATGACAT CAAGGAATAC TTCGGTGTCA AAATTGGCTT GTACTTCGCC 
TGGCTGGGCT ACTACACCTA CATGTTGCTG CTGGCCTCAA TAGTGGGTGT CATTTGTTTC 
CTATATTCAT GGTTCTCCCT TAAAAACTAT GTGCCTGTGA AAGATATTTG CCAGAGCGGT 
AATACAAACA TAACCATGTG TCCTCTTTGT GATTGGTGCA ACTTTTGGGA CCTGAAAGAG 
ACCTGCAACT ATGCCAAAGT CACGTACCTC ATTGACAATC CCAGCACCGT GTTCTTCGCC 
GTTTTTATGT CCTTTTGGGC CACCCTATTT CTGGAGCTGT GGAAGCGGTA CTCCGCCGAA 
ATAACGCATC GTTGGGATCT GACGGGATTC GATGTGCATG AAGAGCATCC GAGGCCACAA 
TATCTGGCGC GTTTGGAGCA CATACCACCG ACGAGAGTGG ATTATGTGAC CAATATAAAA 
GAGCCAACGG TTCCCTTTTG GCGTATGAAA CTTCCCGCCA CCGTGTTCAG CTTTTCAGTG 
GTACTGCTCC TAATTGCACT GGCCTTTGTG GCACTGCTGG CAGTGGTTGT ATACCGAATG 
TCCATGCTGG CCGCCCTTAA AGTGGGTGCT AGTCCCATGA CCACCTCTAG CGCTATTGTC 
CTAGCCACTG CATCAGCTGC CTTTGTAAAT CTGTGCCTGC TCTATATACT TAATTATATG 
TACAATCATT TGGCTGAGTA CCTGACAGAG CTGGAAATGT GGCGCACTCA AACTCAGTTC 
GATGACTCGC TTACCCTTAA AATTTATCTG CTGCAGTTTG TAAACTACTA CGCCTCCATT 
TTTTACATAG CTTTCTTCAA GGGTAAATTC GTTGGTCATC CGGGAGAGTA TAATAAGCTT 
TTTGACTATC GGCAGGAGGA GTGCTCATCG GGCGGCTGTT TGACGGAGCT GTGCATCCAG 
TTAGCCATTA TAATGGTTGG CAAGCAGGCA TTCAACACTA TTCTTGAAGT GTATCTTCCC 
ATGTTCTGGC GAAAGGTTTT GGCCATTCAG GTGGGACTGT CGCGACTTTT CAACAACACC 
CCGAATCCAG ACAAGGCGAA AGACGAACGC TGGATGCGGG ATTTCAAGCT ACTGGATTGG 
GGTGCCCGAG GTCTGTTTCC CGAGTATTTG GAGATGGTCT TGCAGTACGG CTTCGTAACC 
ATCTTTGTGG CCGCTTTTCC GCTGGCGCCA TTCTTTGCCC TGCTAAATAA TATCCTGGAA 
ATGCGACTGG ATGCAAAGAA ACTATTGACC CACCACAAGC GTCCAGTATC ACAGCGAGTT 
CGAGATATAG GAGTGTGGTA TCGTATCCTG GACTGCATAG GCAAGCTCAG CGTGATCACA 
AATGGATTCA TCATTGCCTT TACCTCTGAC ATGATACCGC GGTTGGTGTA CCGGCATTAC 
GTAAACAAGC AAGGCACTCT GGATGGTTAC CTCAACTTTA CACTATCAGA GTTCAAAGTC 
ATTGATTCGC CCACGTTGTA TAGCTTAGCT GGCGATCTCT CCAACATAAC AACGTGCAGA 
TATACGGACT TTCGACTTCC ACCATCGTCT CCAGAAAAAT ACACATTGAG CAGCATGTTC 
TACATAATTC TGGCCTGCCG ATTGGGTTTT GTAGTCGTCT TTGAAAACTT TGTTGCCCTG 
GTGATGATTT TGGTGCGTTG GTGCATTCCG GACATGAGCG TTGAACTGCG TGACCAAATC 
CGACGCGAAG TTTATGTGAC CAATGAAATT ATCATCGATC AAGAGGCCCA CAGGGCTCGT 
TTTGAGCGTG CCAAGCGCAG CAGTTCGGTG CTCCAGACGC CAGTTGACCA GGACATGGAT 
GCTGCCTCCA TTCAAGAGCC TAATGCCAAG TTCATTAACA TTGAAAAGCT CCTTACTGAA 
AATCTCACAC AGATCGAAAT GGATGCTATT ATACACGGCG AAAATCCAAT CACAGGAATT 
TCCGGTGCCG ATTGCTTAGA CGAAGTGCAC AAAAAACTCA GTGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650820.1
CDS	308..3535
Translation	MLNLGSAKVGALEEATSPPPFSKDESFQLLERSPHNPAISPTTTVPLEPPFTTPLAPFDMELMKRHNLHERLTGKESFWRGRDTSQRFLDQEQLSASRESLGAADKRARFAESNAPTLQLPESAEPRANGKRPTGNRTNPSNPAAPLLPMTDPDFVKGNVGGVYDDRECSCPREFYQRGEINTSLFFEDCVRSIDFVLAYRINAHEPTELENTEKRRVFEANLISQGLEVESSQKDQIWFVKIHAPLEVLRRYAEILKLRMPMKEIPGMSVVNRSTKSVFSSLKHVFQFFLRNIYVDEEIFPKRAHRFTAIYSRDKEYLFDIRQDCFFTTAVRSRIVEFILDRQRFPAKNQHDMAFGIERLIAEGVYSAAYPLHDGEITETGTMRALLYKHWASVPKWYRYQPLDDIKEYFGVKIGLYFAWLGYYTYMLLLASIVGVICFLYSWFSLKNYVPVKDICQSGNTNITMCPLCDWCNFWDLKETCNYAKVTYLIDNPSTVFFAVFMSFWATLFLELWKRYSAEITHRWDLTGFDVHEEHPRPQYLARLEHIPPTRVDYVTNIKEPTVPFWRMKLPATVFSFSVVLLLIALAFVALLAVVVYRMSMLAALKVGASPMTTSSAIVLATASAAFVNLCLLYILNYMYNHLAEYLTELEMWRTQTQFDDSLTLKIYLLQFVNYYASIFYIAFFKGKFVGHPGEYNKLFDYRQEECSSGGCLTELCIQLAIIMVGKQAFNTILEVYLPMFWRKVLAIQVGLSRLFNNTPNPDKAKDERWMRDFKLLDWGARGLFPEYLEMVLQYGFVTIFVAAFPLAPFFALLNNILEMRLDAKKLLTHHKRPVSQRVRDIGVWYRILDCIGKLSVITNGFIIAFTSDMIPRLVYRHYVNKQGTLDGYLNFTLSEFKVIDSPTLYSLAGDLSNITTCRYTDFRLPPSSPEKYTLSSMFYIILACRLGFVVVFENFVALVMILVRWCIPDMSVELRDQIRREVYVTNEIIIDQEAHRARFERAKRSSSVLQTPVDQDMDAASIQEPNAKFINIEKLLTENLTQIEMDAIIHGENPITGISGADCLDEVHKKLSD

Target ORF information:

RefSeq Version	NM_142563.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster subdued, transcript variant A (subdued), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142563.2

ORF Insert Sequence:

ATGCTAAATT TAGGCAGTGC CAAAGTTGGC GCTCTGGAGG AGGCCACCTC ACCACCCCCC 
TTCTCCAAGG ACGAGAGCTT CCAGCTGTTG GAGCGGAGCC CCCACAATCC CGCCATATCA 
CCCACCACCA CTGTCCCGCT GGAGCCCCCC TTCACCACCC CCCTAGCTCC CTTCGACATG 
GAGCTGATGA AGCGCCACAA TCTCCACGAG CGCTTAACGG GAAAGGAGAG CTTCTGGCGC 
GGGCGCGACA CTTCGCAGAG ATTTCTGGAC CAGGAGCAAT TATCAGCATC GCGTGAATCA 
CTCGGTGCCG CCGACAAGAG AGCCCGGTTT GCAGAAAGCA ACGCCCCCAC GCTCCAACTG 
CCAGAATCTG CCGAGCCCAG GGCCAATGGC AAAAGACCCA CTGGGAACAG GACCAACCCA 
TCGAATCCAG CCGCTCCCCT TCTGCCAATG ACTGATCCGG ATTTTGTGAA GGGCAATGTA 
GGCGGCGTCT ACGATGATCG CGAGTGCAGC TGCCCCAGGG AGTTCTACCA GCGGGGCGAG 
ATTAACACAT CACTGTTCTT TGAGGACTGC GTCCGGTCCA TTGACTTTGT CCTGGCCTAC 
AGGATCAATG CGCACGAGCC CACGGAGCTG GAGAACACGG AAAAGCGTCG TGTTTTCGAG 
GCGAATCTCA TTAGTCAGGG GCTGGAGGTC GAGTCCAGTC AGAAGGACCA GATCTGGTTC 
GTTAAGATCC ACGCTCCTTT GGAGGTCCTG CGACGGTATG CGGAAATTCT TAAGCTGCGC 
ATGCCCATGA AGGAGATTCC AGGAATGTCC GTGGTCAATC GATCGACCAA AAGCGTGTTT 
TCTAGTCTTA AACATGTTTT CCAATTCTTT CTGCGCAACA TTTATGTGGA CGAGGAAATT 
TTTCCAAAGC GAGCTCATCG ATTCACTGCC ATTTACAGTC GCGATAAGGA GTATCTCTTT 
GACATCCGAC AGGATTGTTT CTTTACCACC GCCGTCCGTT CCCGCATCGT TGAGTTCATC 
CTAGATCGCC AGCGATTTCC TGCCAAGAAT CAACATGACA TGGCCTTCGG TATTGAGCGA 
CTGATTGCCG AGGGTGTCTA CTCTGCTGCC TATCCACTCC ATGATGGTGA AATAACCGAG 
ACGGGAACCA TGAGAGCTCT GCTGTACAAA CATTGGGCCT CTGTGCCCAA ATGGTATCGC 
TATCAGCCCT TGGATGACAT CAAGGAATAC TTCGGTGTCA AAATTGGCTT GTACTTCGCC 
TGGCTGGGCT ACTACACCTA CATGTTGCTG CTGGCCTCAA TAGTGGGTGT CATTTGTTTC 
CTATATTCAT GGTTCTCCCT TAAAAACTAT GTGCCTGTGA AAGATATTTG CCAGAGCGGT 
AATACAAACA TAACCATGTG TCCTCTTTGT GATTGGTGCA ACTTTTGGGA CCTGAAAGAG 
ACCTGCAACT ATGCCAAAGT CACGTACCTC ATTGACAATC CCAGCACCGT GTTCTTCGCC 
GTTTTTATGT CCTTTTGGGC CACCCTATTT CTGGAGCTGT GGAAGCGGTA CTCCGCCGAA 
ATAACGCATC GTTGGGATCT GACGGGATTC GATGTGCATG AAGAGCATCC GAGGCCACAA 
TATCTGGCGC GTTTGGAGCA CATACCACCG ACGAGAGTGG ATTATGTGAC CAATATAAAA 
GAGCCAACGG TTCCCTTTTG GCGTATGAAA CTTCCCGCCA CCGTGTTCAG CTTTTCAGTG 
GTACTGCTCC TAATTGCACT GGCCTTTGTG GCACTGCTGG CAGTGGTTGT ATACCGAATG 
TCCATGCTGG CCGCCCTTAA AGTGGGTGCT AGTCCCATGA CCACCTCTAG CGCTATTGTC 
CTAGCCACTG CATCAGCTGC CTTTGTAAAT CTGTGCCTGC TCTATATACT TAATTATATG 
TACAATCATT TGGCTGAGTA CCTGACAGAG CTGGAAATGT GGCGCACTCA AACTCAGTTC 
GATGACTCGC TTACCCTTAA AATTTATCTG CTGCAGTTTG TAAACTACTA CGCCTCCATT 
TTTTACATAG CTTTCTTCAA GGGTAAATTC GTTGGTCATC CGGGAGAGTA TAATAAGCTT 
TTTGACTATC GGCAGGAGGA GTGCTCATCG GGCGGCTGTT TGACGGAGCT GTGCATCCAG 
TTAGCCATTA TAATGGTTGG CAAGCAGGCA TTCAACACTA TTCTTGAAGT GTATCTTCCC 
ATGTTCTGGC GAAAGGTTTT GGCCATTCAG GTGGGACTGT CGCGACTTTT CAACAACACC 
CCGAATCCAG ACAAGGCGAA AGACGAACGC TGGATGCGGG ATTTCAAGCT ACTGGATTGG 
GGTGCCCGAG GTCTGTTTCC CGAGTATTTG GAGATGGTCT TGCAGTACGG CTTCGTAACC 
ATCTTTGTGG CCGCTTTTCC GCTGGCGCCA TTCTTTGCCC TGCTAAATAA TATCCTGGAA 
ATGCGACTGG ATGCAAAGAA ACTATTGACC CACCACAAGC GTCCAGTATC ACAGCGAGTT 
CGAGATATAG GAGTGTGGTA TCGTATCCTG GACTGCATAG GCAAGCTCAG CGTGATCACA 
AATGGATTCA TCATTGCCTT TACCTCTGAC ATGATACCGC GGTTGGTGTA CCGGCATTAC 
GTAAACAAGC AAGGCACTCT GGATGGTTAC CTCAACTTTA CACTATCAGA GTTCAAAGTC 
ATTGATTCGC CCACGTTGTA TAGCTTAGCT GGCGATCTCT CCAACATAAC AACGTGCAGA 
TATACGGACT TTCGACTTCC ACCATCGTCT CCAGAAAAAT ACACATTGAG CAGCATGTTC 
TACATAATTC TGGCCTGCCG ATTGGGTTTT GTAGTCGTCT TTGAAAACTT TGTTGCCCTG 
GTGATGATTT TGGTGCGTTG GTGCATTCCG GACATGAGCG TTGAACTGCG TGACCAAATC 
CGACGCGAAG TTTATGTGAC CAATGAAATT ATCATCGATC AAGAGGCCCA CAGGGCTCGT 
TTTGAGCGTG CCAAGCGCAG CAGTTCGGTG CTCCAGACGC CAGTTGACCA GGACATGGAT 
GCTGCCTCCA TTCAAGAGCC TAATGCCAAG TTCATTAACA TTGAAAAGCT CCTTACTGAA 
AATCTCACAC AGATCGAAAT GGATGCTATT ATACACGGCG AAAATCCAAT CACAGGAATT 
TCCGGTGCCG ATTGCTTAGA CGAAGTGCAC AAAAAACTCA GTGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

subdued ( NM_001202319.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189248.1 Drosophila melanogaster subdued, transcript variant D (subdued), mRNA.

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CloneID	OFa07808
Clone ID Related Accession (Same CDS sequence)	NM_001202319.2
Accession Version	NM_001202319.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	subdued, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001202319.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTAAATT TAGGCAGTGC CAAAGTTGGC GCTCTGGAGG AGGCCACCTC ACCACCCCCC 
TTCTCCAAGG ACGAGAGCTT CCAGCTGTTG GAGCGGAGCC CCCACAATCC CGCCATATCA 
CCCACCACCA CTGTCCCGCT GGAGCCCCCC TTCACCACCC CCCTAGCTCC CTTCGACATG 
GAGCTGATGA AGCGCCACAA TCTCCACGAG CGCTTAACGG GAAAGGAGAG CTTCTGGCGC 
GGGCGCGACA CTTCGCAGAG ATTTCTGGAC CAGGAGCAAT TATCAGCATC GCGTGAATCA 
CTCGGTGCCG CCGACAAGAG AGCCCGGTTT GCAGAAAGCA ACGCCCCCAC GCTCCAACTG 
CCAGAATCTG CCGAGCCCAG GGCCAATGGC AAAAGACCCA CTGGGAACAG GACCAACCCA 
TCGAATCCAG CCGCTCCCCT TCTGCCAATG ACTGATCCGG ATTTTGTGAA GGGCAATGTA 
GGCGGCGTCT ACGATGATCG CGAGTGCAGC TGCCCCAGGG AGTTCTACCA GCGGGGCGAG 
ATTAACACAT CACTGTTCTT TGAGGACTGC GTCCGGTCCA TTGACTTTGT CCTGGCCTAC 
AGGATCAATG CGCACGAGCC CACGGAGCTG GAGAACACGG AAAAGCGTCG TGTTTTCGAG 
GCGAATCTCA TTAGTCAGGG GCTGGAGGTC GAGTCCAGTC AGAAGGACCA GATCTGGTTC 
GTTAAGATCC ACGCTCCTTT GGAGGTCCTG CGACGGTATG CGGAAATTCT TAAGCTGCGC 
ATGCCCATGA AGGAGTCGTT ATGCAATTTA CGCATATCTG AAAGATCGAA TCGCCTACGA 
AATGCGGCCC ATTACTTGAC CAATAAGATT CCAGGAATGT CCGTGGTCAA TCGATCGACC 
AAAAGCGTGT TTTCTAGTCT TAAACATGTT TTCCAATTCT TTCTGCGCAA CATTTATGTG 
GACGAGGAAA TTTTTCCAAA GCGAGCTCAT CGATTCACTG CCATTTACAG TCGCGATAAG 
GAGTATCTCT TTGACATCCG ACAGGATTGT TTCTTTACCA CCGCCGTCCG TTCCCGCATC 
GTTGAGTTCA TCCTAGATCG CCAGCGATTT CCTGCCAAGA ATCAACATGA CATGGCCTTC 
GGTATTGAGC GACTGATTGC CGAGGGTGTC TACTCTGCTG CCTATCCACT CCATGATGGT 
GAAATAACCG AGACGGGAAC CATGAGAGCT CTGCTGTACA AACATTGGGC CTCTGTGCCC 
AAATGGTATC GCTATCAGCC CTTGGATGAC ATCAAGGAAT ACTTCGGTGT CAAAATTGGC 
TTGTACTTCG CCTGGCTGGG CTACTACACC TACATGTTGC TGCTGGCCTC AATAGTGGGT 
GTCATTTGTT TCCTATATTC ATGGTTCTCC CTTAAAAACT ATGTGCCTGT GAAAGATATT 
TGCCAGAGCG GTAATACAAA CATAACCATG TGTCCTCTTT GTGATTGGTG CAACTTTTGG 
GACCTGAAAG AGACCTGCAA CTATGCCAAA GTCACGTACC TCATTGACAA TCCCAGCACC 
GTGTTCTTCG CCGTTTTTAT GTCCTTTTGG GCCACCCTAT TTCTGGAGCT GTGGAAGCGG 
TACTCCGCCG AAATAACGCA TCGTTGGGAT CTGACGGGAT TCGATGTGCA TGAAGAGCAT 
CCGAGGCCAC AATATCTGGC GCGTTTGGAG CACATACCAC CGACGAGAGT GGATTATGTG 
ACCAATATAA AAGAGCCAAC GGTTCCCTTT TGGCGTATGA AACTTCCCGC CACCGTGTTC 
AGCTTTTCAG TGGTACTGCT CCTAATTGCA CTGGCCTTTG TGGCACTGCT GGCAGTGGTT 
GTATACCGAA TGTCCATGCT GGCCGCCCTT AAAGTGGGTG CTAGTCCCAT GACCACCTCT 
AGCGCTATTG TCCTAGCCAC TGCATCAGCT GCCTTTGTAA ATCTGTGCCT GCTCTATATA 
CTTAATTATA TGTACAATCA TTTGGCTGAG TACCTGACAG AGCTGGAAAT GTGGCGCACT 
CAAACTCAGT TCGATGACTC GCTTACCCTT AAAATTTATC TGCTGCAGTT TGTAAACTAC 
TACGCCTCCA TTTTTTACAT AGCTTTCTTC AAGGGTAAAT TCGTTGGTCA TCCGGGAGAG 
TATAATAAGC TTTTTGACTA TCGGCAGGAG GAGTGCTCAT CGGGCGGCTG TTTGACGGAG 
CTGTGCATCC AGTTAGCCAT TATAATGGTT GGCAAGCAGG CATTCAACAC TATTCTTGAA 
GTGTATCTTC CCATGTTCTG GCGAAAGGTT TTGGCCATTC AGGTGGGACT GTCGCGACTT 
TTCAACAACA CCCCGAATCC AGACAAGGCG AAAGACGAAC GCTGGATGCG GGATTTCAAG 
CTACTGGATT GGGGTGCCCG AGGTCTGTTT CCCGAGTATT TGGAGATGGT CTTGCAGTAC 
GGCTTCGTAA CCATCTTTGT GGCCGCTTTT CCGCTGGCGC CATTCTTTGC CCTGCTAAAT 
AATATCCTGG AAATGCGACT GGATGCAAAG AAACTATTGA CCCACCACAA GCGTCCAGTA 
TCACAGCGAG TTCGAGATAT AGGAGTGTGG TATCGTATCC TGGACTGCAT AGGCAAGCTC 
AGCGTGATCA CAAATGGATT CATCATTGCC TTTACCTCTG ACATGATACC GCGGTTGGTG 
TACCGGCATT ACGTAAACAA GCAAGGCACT CTGGATGGTT ACCTCAACTT TACACTATCA 
GAGTTCAAAG TCATTGATTC GCCCACGTTG TATAGCTTAG CTGGCGATCT CTCCAACATA 
ACAACGTGCA GATATACGGA CTTTCGACTT CCACCATCGT CTCCAGAAAA ATACACATTG 
AGCAGCATGT TCTACATAAT TCTGGCCTGC CGATTGGGTT TTGTAGTCGT CTTTGAAAAC 
TTTGTTGCCC TGGTGATGAT TTTGGTGCGT TGGTGCATTC CGGACATGAG CGTTGAACTG 
CGTGACCAAA TCCGACGCGA AGTTTATGTG ACCAATGAAA TTATCATCGA TCAAGAGGCC 
CACAGGGCTC GTTTTGAGCG TGCCAAGCGC AGCAGTTCGG TGCTCCAGAC GCCAGTTGAC 
CAGGACATGG ATGCTGCCTC CATTCAAGAG CCTAATGCCA AGTTCATTAA CATTGAAAAG 
CTCCTTACTG AAAATCTCAC ACAGATCGAA ATGGATGCTA TTATACACGG CGAAAATCCA 
ATCACAGGAA TTTCCGGTGC CGATTGCTTA GACGAAGTGC ACAAAAAACT CAGTGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189248.1
CDS	308..3607
Translation	MLNLGSAKVGALEEATSPPPFSKDESFQLLERSPHNPAISPTTTVPLEPPFTTPLAPFDMELMKRHNLHERLTGKESFWRGRDTSQRFLDQEQLSASRESLGAADKRARFAESNAPTLQLPESAEPRANGKRPTGNRTNPSNPAAPLLPMTDPDFVKGNVGGVYDDRECSCPREFYQRGEINTSLFFEDCVRSIDFVLAYRINAHEPTELENTEKRRVFEANLISQGLEVESSQKDQIWFVKIHAPLEVLRRYAEILKLRMPMKESLCNLRISERSNRLRNAAHYLTNKIPGMSVVNRSTKSVFSSLKHVFQFFLRNIYVDEEIFPKRAHRFTAIYSRDKEYLFDIRQDCFFTTAVRSRIVEFILDRQRFPAKNQHDMAFGIERLIAEGVYSAAYPLHDGEITETGTMRALLYKHWASVPKWYRYQPLDDIKEYFGVKIGLYFAWLGYYTYMLLLASIVGVICFLYSWFSLKNYVPVKDICQSGNTNITMCPLCDWCNFWDLKETCNYAKVTYLIDNPSTVFFAVFMSFWATLFLELWKRYSAEITHRWDLTGFDVHEEHPRPQYLARLEHIPPTRVDYVTNIKEPTVPFWRMKLPATVFSFSVVLLLIALAFVALLAVVVYRMSMLAALKVGASPMTTSSAIVLATASAAFVNLCLLYILNYMYNHLAEYLTELEMWRTQTQFDDSLTLKIYLLQFVNYYASIFYIAFFKGKFVGHPGEYNKLFDYRQEECSSGGCLTELCIQLAIIMVGKQAFNTILEVYLPMFWRKVLAIQVGLSRLFNNTPNPDKAKDERWMRDFKLLDWGARGLFPEYLEMVLQYGFVTIFVAAFPLAPFFALLNNILEMRLDAKKLLTHHKRPVSQRVRDIGVWYRILDCIGKLSVITNGFIIAFTSDMIPRLVYRHYVNKQGTLDGYLNFTLSEFKVIDSPTLYSLAGDLSNITTCRYTDFRLPPSSPEKYTLSSMFYIILACRLGFVVVFENFVALVMILVRWCIPDMSVELRDQIRREVYVTNEIIIDQEAHRARFERAKRSSSVLQTPVDQDMDAASIQEPNAKFINIEKLLTENLTQIEMDAIIHGENPITGISGADCLDEVHKKLSD

Target ORF information:

RefSeq Version	NM_001202319.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster subdued, transcript variant D (subdued), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202319.2

ORF Insert Sequence:

ATGCTAAATT TAGGCAGTGC CAAAGTTGGC GCTCTGGAGG AGGCCACCTC ACCACCCCCC 
TTCTCCAAGG ACGAGAGCTT CCAGCTGTTG GAGCGGAGCC CCCACAATCC CGCCATATCA 
CCCACCACCA CTGTCCCGCT GGAGCCCCCC TTCACCACCC CCCTAGCTCC CTTCGACATG 
GAGCTGATGA AGCGCCACAA TCTCCACGAG CGCTTAACGG GAAAGGAGAG CTTCTGGCGC 
GGGCGCGACA CTTCGCAGAG ATTTCTGGAC CAGGAGCAAT TATCAGCATC GCGTGAATCA 
CTCGGTGCCG CCGACAAGAG AGCCCGGTTT GCAGAAAGCA ACGCCCCCAC GCTCCAACTG 
CCAGAATCTG CCGAGCCCAG GGCCAATGGC AAAAGACCCA CTGGGAACAG GACCAACCCA 
TCGAATCCAG CCGCTCCCCT TCTGCCAATG ACTGATCCGG ATTTTGTGAA GGGCAATGTA 
GGCGGCGTCT ACGATGATCG CGAGTGCAGC TGCCCCAGGG AGTTCTACCA GCGGGGCGAG 
ATTAACACAT CACTGTTCTT TGAGGACTGC GTCCGGTCCA TTGACTTTGT CCTGGCCTAC 
AGGATCAATG CGCACGAGCC CACGGAGCTG GAGAACACGG AAAAGCGTCG TGTTTTCGAG 
GCGAATCTCA TTAGTCAGGG GCTGGAGGTC GAGTCCAGTC AGAAGGACCA GATCTGGTTC 
GTTAAGATCC ACGCTCCTTT GGAGGTCCTG CGACGGTATG CGGAAATTCT TAAGCTGCGC 
ATGCCCATGA AGGAGTCGTT ATGCAATTTA CGCATATCTG AAAGATCGAA TCGCCTACGA 
AATGCGGCCC ATTACTTGAC CAATAAGATT CCAGGAATGT CCGTGGTCAA TCGATCGACC 
AAAAGCGTGT TTTCTAGTCT TAAACATGTT TTCCAATTCT TTCTGCGCAA CATTTATGTG 
GACGAGGAAA TTTTTCCAAA GCGAGCTCAT CGATTCACTG CCATTTACAG TCGCGATAAG 
GAGTATCTCT TTGACATCCG ACAGGATTGT TTCTTTACCA CCGCCGTCCG TTCCCGCATC 
GTTGAGTTCA TCCTAGATCG CCAGCGATTT CCTGCCAAGA ATCAACATGA CATGGCCTTC 
GGTATTGAGC GACTGATTGC CGAGGGTGTC TACTCTGCTG CCTATCCACT CCATGATGGT 
GAAATAACCG AGACGGGAAC CATGAGAGCT CTGCTGTACA AACATTGGGC CTCTGTGCCC 
AAATGGTATC GCTATCAGCC CTTGGATGAC ATCAAGGAAT ACTTCGGTGT CAAAATTGGC 
TTGTACTTCG CCTGGCTGGG CTACTACACC TACATGTTGC TGCTGGCCTC AATAGTGGGT 
GTCATTTGTT TCCTATATTC ATGGTTCTCC CTTAAAAACT ATGTGCCTGT GAAAGATATT 
TGCCAGAGCG GTAATACAAA CATAACCATG TGTCCTCTTT GTGATTGGTG CAACTTTTGG 
GACCTGAAAG AGACCTGCAA CTATGCCAAA GTCACGTACC TCATTGACAA TCCCAGCACC 
GTGTTCTTCG CCGTTTTTAT GTCCTTTTGG GCCACCCTAT TTCTGGAGCT GTGGAAGCGG 
TACTCCGCCG AAATAACGCA TCGTTGGGAT CTGACGGGAT TCGATGTGCA TGAAGAGCAT 
CCGAGGCCAC AATATCTGGC GCGTTTGGAG CACATACCAC CGACGAGAGT GGATTATGTG 
ACCAATATAA AAGAGCCAAC GGTTCCCTTT TGGCGTATGA AACTTCCCGC CACCGTGTTC 
AGCTTTTCAG TGGTACTGCT CCTAATTGCA CTGGCCTTTG TGGCACTGCT GGCAGTGGTT 
GTATACCGAA TGTCCATGCT GGCCGCCCTT AAAGTGGGTG CTAGTCCCAT GACCACCTCT 
AGCGCTATTG TCCTAGCCAC TGCATCAGCT GCCTTTGTAA ATCTGTGCCT GCTCTATATA 
CTTAATTATA TGTACAATCA TTTGGCTGAG TACCTGACAG AGCTGGAAAT GTGGCGCACT 
CAAACTCAGT TCGATGACTC GCTTACCCTT AAAATTTATC TGCTGCAGTT TGTAAACTAC 
TACGCCTCCA TTTTTTACAT AGCTTTCTTC AAGGGTAAAT TCGTTGGTCA TCCGGGAGAG 
TATAATAAGC TTTTTGACTA TCGGCAGGAG GAGTGCTCAT CGGGCGGCTG TTTGACGGAG 
CTGTGCATCC AGTTAGCCAT TATAATGGTT GGCAAGCAGG CATTCAACAC TATTCTTGAA 
GTGTATCTTC CCATGTTCTG GCGAAAGGTT TTGGCCATTC AGGTGGGACT GTCGCGACTT 
TTCAACAACA CCCCGAATCC AGACAAGGCG AAAGACGAAC GCTGGATGCG GGATTTCAAG 
CTACTGGATT GGGGTGCCCG AGGTCTGTTT CCCGAGTATT TGGAGATGGT CTTGCAGTAC 
GGCTTCGTAA CCATCTTTGT GGCCGCTTTT CCGCTGGCGC CATTCTTTGC CCTGCTAAAT 
AATATCCTGG AAATGCGACT GGATGCAAAG AAACTATTGA CCCACCACAA GCGTCCAGTA 
TCACAGCGAG TTCGAGATAT AGGAGTGTGG TATCGTATCC TGGACTGCAT AGGCAAGCTC 
AGCGTGATCA CAAATGGATT CATCATTGCC TTTACCTCTG ACATGATACC GCGGTTGGTG 
TACCGGCATT ACGTAAACAA GCAAGGCACT CTGGATGGTT ACCTCAACTT TACACTATCA 
GAGTTCAAAG TCATTGATTC GCCCACGTTG TATAGCTTAG CTGGCGATCT CTCCAACATA 
ACAACGTGCA GATATACGGA CTTTCGACTT CCACCATCGT CTCCAGAAAA ATACACATTG 
AGCAGCATGT TCTACATAAT TCTGGCCTGC CGATTGGGTT TTGTAGTCGT CTTTGAAAAC 
TTTGTTGCCC TGGTGATGAT TTTGGTGCGT TGGTGCATTC CGGACATGAG CGTTGAACTG 
CGTGACCAAA TCCGACGCGA AGTTTATGTG ACCAATGAAA TTATCATCGA TCAAGAGGCC 
CACAGGGCTC GTTTTGAGCG TGCCAAGCGC AGCAGTTCGG TGCTCCAGAC GCCAGTTGAC 
CAGGACATGG ATGCTGCCTC CATTCAAGAG CCTAATGCCA AGTTCATTAA CATTGAAAAG 
CTCCTTACTG AAAATCTCAC ACAGATCGAA ATGGATGCTA TTATACACGG CGAAAATCCA 
ATCACAGGAA TTTCCGGTGC CGATTGCTTA GACGAAGTGC ACAAAAAACT CAGTGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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subdued cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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