nos cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	nos
Entrez Gene ID	42297
Full Name	nanos
Synonyms	CG5637,DRONANOS,Dmel\CG5637,Dronanos,NANOS,NOS,Nanos,Nos,l(3)07117,l(3)j3B6,l(3)j4B6,nNOS
General protein information	Preferred Names nanos Names CG5637 gene product from transcript CG5637-RA CG5637-PA CG5637-PB nos-PA nos-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 91F4-91F4\|3-66.2 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275794.1	NP_001262723.1	nanos, isoform B
NM_057310.4	NP_476658.1	nanos, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Translational repression of the Drosophila nanos mRNA involves the RNA helicase Belle and RNA coating by Me31B and Trailer hitch.Bel is required for nos mRNA translational repression.
Title: Translational repression of the

Results showed that nos mRNA, one of the target mRNAs of Hrp38 identified in this study, is regulated by Hrp38 for translational control during ovary development.
Title: Poly(ADP-Ribosyl)ation of hnRNP A1 Protein Controls Translational Repression in Drosophila.

TomO enhances the maintenance of germ stem cells (GSCs) by elevating Nanos (Nos) expression via its interaction with nos mRNA, ultimately leading to the restoration of germ cell production in Sxl mutant females that are otherwise without GSCs.[
Title: Wolbachia Protein TomO Targets nanos mRNA and Restores Germ Stem Cells in Drosophila Sex-lethal Mutants.

Crystal structures of Nanos-Pumilio-RNA complexes reveal that Nanos embraces Pumilio and RNA, contributes sequence-specific contacts, and increases Pumilio RNA-binding affinity.
Title: Drosophila Nanos acts as a molecular clamp that modulates the RNA-binding and repression activities of Pumilio.

hid is required during pupariation for caspase-dependent pruning of class IV da neurons and Nos and Pum delay pruning.
Title: Nanos-mediated repression of hid protects larval sensory neurons after a global switch in sensitivity to apoptotic signals.

The following nos gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nos cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05380	NM_057310.4 Latest version!	Drosophila melanogaster nanos (nos), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa07796	NM_001275794.1 Latest version!	Drosophila melanogaster nanos (nos), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nos ( NM_057310.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476658.1 Drosophila melanogaster nanos (nos), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa05380
Clone ID Related Accession (Same CDS sequence)	NM_057310.4
Accession Version	NM_057310.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nanos, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_057310.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCCGCA GCAACTTGGA GGGCAGTGGC GCAGCAGCAG TAGGTGTTGC AAATCCCCCC 
TCGTTGGCTC AGTCTGGAAA GATTTTCCAA TTGCAGGATA ACTTTTCTGC TTTTCACGCC 
AGAGGAGGGC TCAACATTCT GGGCCTGCAG GACATGTATT TGGATACCAG TGGGGCCAAC 
TCGTCGGCCA CTTTGAGTCC GCCCATTACG CCGGTGACCC CTGACCCGTC GACGTCTGCG 
CAGTCGACGC ACTTCCCTTT TCTGGCCGAC AGCGCAGCCA CCGCCAATTC GCTCCTTATG 
CAGCGACAGT ACCACTACCA CTTGCTGCTC CAGCAGCAGC AGCAACTGGC CATGGCGCAG 
CACCAATTGG CGCTGGCTGC ATCAGCGGCA GCGGCTAGTG CGAGTCACCA GCAAACGGAC 
GAGATTGCGC GATCCTTGAA AATCTTTGCG CAGGTGACGA CGGGCGCAGC AGAAAATGCG 
GCTGGCTCGA TGCAGGATGT GATGCAGGAG TTCGCGACCA ATGGCTATGC CAGCGATGAT 
CTCGGTCGCA TGTCCTACGG GAGTGCTCCG CCACAGGTGC AAATGCCACC GCAGCAGCAG 
CATCAGCAAC AGCAGGGGCT GCACCTGCCA CTGGGCCGCA ATCCTGCCCA GCTGCAGACC 
AATGGCGGCA ACTTAATGCC CATTCCACTC GCCACCCACT GGCTGAACAA CTACCGGGAG 
CATCTGAACA ACGTGTGGCG AAACATGTCG TATATACCAG CCGCTCCCAA TACAATGGGT 
TTGCAGGCCC AAACAGCGGC CACTGTGTCC ACCAATCTCG GCGTGGGAAT GGGTCTGGGA 
TTGCCCGTGC AGGGCGAACA GCTGCGCGGA GCTTCCAATT CCAGTAACAA TAATAACAAC 
AACAACAAGG TGTACAAGCG TTACAACAGC AAGGCCAAAG AGATCAGCCG CCACTGCGTC 
TTTTGTGAGA ATAACAACGA ACCAGAGGCG GTTATCAATA GCCACTCAGT GCGAGATAAC 
TTTAACCGAG TGCTGTGCCC CAAACTACGC ACCTACGTGT GCCCCATCTG CGGGGCATCT 
GGGGACTCGG CGCACACGAT TAAGTACTGC CCCAAGAAGC CGATCATCAC CATGGAGGAT 
GCGATCAAGG CGGAATCGTT CCGCCTAGCC AAGAGCAGTT ACTACAAGCA ACAGATGAAG 
GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476658.1
CDS	264..1469
Translation	MFRSNLEGSGAAAVGVANPPSLAQSGKIFQLQDNFSAFHARGGLNILGLQDMYLDTSGANSSATLSPPITPVTPDPSTSAQSTHFPFLADSAATANSLLMQRQYHYHLLLQQQQQLAMAQHQLALAASAAAASASHQQTDEIARSLKIFAQVTTGAAENAAGSMQDVMQEFATNGYASDDLGRMSYGSAPPQVQMPPQQQHQQQQGLHLPLGRNPAQLQTNGGNLMPIPLATHWLNNYREHLNNVWRNMSYIPAAPNTMGLQAQTAATVSTNLGVGMGLGLPVQGEQLRGASNSSNNNNNNNKVYKRYNSKAKEISRHCVFCENNNEPEAVINSHSVRDNFNRVLCPKLRTYVCPICGASGDSAHTIKYCPKKPIITMEDAIKAESFRLAKSSYYKQQMKV

Target ORF information:

RefSeq Version	NM_057310.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nanos (nos), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057310.4

ORF Insert Sequence:

ATGTTCCGCA GCAACTTGGA GGGCAGTGGC GCAGCAGCAG TAGGTGTTGC AAATCCCCCC 
TCGTTGGCTC AGTCTGGAAA GATTTTCCAA TTGCAGGATA ACTTTTCTGC TTTTCACGCC 
AGAGGAGGGC TCAACATTCT GGGCCTGCAG GACATGTATT TGGATACCAG TGGGGCCAAC 
TCGTCGGCCA CTTTGAGTCC GCCCATTACG CCGGTGACCC CTGACCCGTC GACGTCTGCG 
CAGTCGACGC ACTTCCCTTT TCTGGCCGAC AGCGCAGCCA CCGCCAATTC GCTCCTTATG 
CAGCGACAGT ACCACTACCA CTTGCTGCTC CAGCAGCAGC AGCAACTGGC CATGGCGCAG 
CACCAATTGG CGCTGGCTGC ATCAGCGGCA GCGGCTAGTG CGAGTCACCA GCAAACGGAC 
GAGATTGCGC GATCCTTGAA AATCTTTGCG CAGGTGACGA CGGGCGCAGC AGAAAATGCG 
GCTGGCTCGA TGCAGGATGT GATGCAGGAG TTCGCGACCA ATGGCTATGC CAGCGATGAT 
CTCGGTCGCA TGTCCTACGG GAGTGCTCCG CCACAGGTGC AAATGCCACC GCAGCAGCAG 
CATCAGCAAC AGCAGGGGCT GCACCTGCCA CTGGGCCGCA ATCCTGCCCA GCTGCAGACC 
AATGGCGGCA ACTTAATGCC CATTCCACTC GCCACCCACT GGCTGAACAA CTACCGGGAG 
CATCTGAACA ACGTGTGGCG AAACATGTCG TATATACCAG CCGCTCCCAA TACAATGGGT 
TTGCAGGCCC AAACAGCGGC CACTGTGTCC ACCAATCTCG GCGTGGGAAT GGGTCTGGGA 
TTGCCCGTGC AGGGCGAACA GCTGCGCGGA GCTTCCAATT CCAGTAACAA TAATAACAAC 
AACAACAAGG TGTACAAGCG TTACAACAGC AAGGCCAAAG AGATCAGCCG CCACTGCGTC 
TTTTGTGAGA ATAACAACGA ACCAGAGGCG GTTATCAATA GCCACTCAGT GCGAGATAAC 
TTTAACCGAG TGCTGTGCCC CAAACTACGC ACCTACGTGT GCCCCATCTG CGGGGCATCT 
GGGGACTCGG CGCACACGAT TAAGTACTGC CCCAAGAAGC CGATCATCAC CATGGAGGAT 
GCGATCAAGG CGGAATCGTT CCGCCTAGCC AAGAGCAGTT ACTACAAGCA ACAGATGAAG 
GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nos ( NM_001275794.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262723.1 Drosophila melanogaster nanos (nos), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa07796
Clone ID Related Accession (Same CDS sequence)	NM_001275794.1
Accession Version	NM_001275794.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nanos, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCCGCA GCAACTTGGA GGGCAGTGGC GCAGCAGCAG ATAACTTTTC TGCTTTTCAC 
GCCAGAGGAG GGCTCAACAT TCTGGGCCTG CAGGACATGT ATTTGGATAC CAGTGGGGCC 
AACTCGTCGG CCACTTTGAG TCCGCCCATT ACGCCGGTGA CCCCTGACCC GTCGACGTCT 
GCGCAGTCGA CGCACTTCCC TTTTCTGGCC GACAGCGCAG CCACCGCCAA TTCGCTCCTT 
ATGCAGCGAC AGTACCACTA CCACTTGCTG CTCCAGCAGC AGCAGCAACT GGCCATGGCG 
CAGCACCAAT TGGCGCTGGC TGCATCAGCG GCAGCGGCTA GTGCGAGTCA CCAGCAAACG 
GACGAGATTG CGCGATCCTT GAAAATCTTT GCGCAGGTGA CGACGGGCGC AGCAGAAAAT 
GCGGCTGGCT CGATGCAGGA TGTGATGCAG GAGTTCGCGA CCAATGGCTA TGCCAGCGAT 
GATCTCGGTC GCATGTCCTA CGGGAGTGCT CCGCCACAGG TGCAAATGCC ACCGCAGCAG 
CAGCATCAGC AACAGCAGGG GCTGCACCTG CCACTGGGCC GCAATCCTGC CCAGCTGCAG 
ACCAATGGCG GCAACTTAAT GCCCATTCCA CTCGCCACCC ACTGGCTGAA CAACTACCGG 
GAGCATCTGA ACAACGTGTG GCGAAACATG TCGTATATAC CAGCCGCTCC CAATACAATG 
GGTTTGCAGG CCCAAACAGC GGCCACTGTG TCCACCAATC TCGGCGTGGG AATGGGTCTG 
GGATTGCCCG TGCAGGGCGA ACAGCTGCGC GGAGCTTCCA ATTCCAGTAA CAATAATAAC 
AACAACAACA AGGTGTACAA GCGTTACAAC AGCAAGGCCA AAGAGATCAG CCGCCACTGC 
GTCTTTTGTG AGAATAACAA CGAACCAGAG GCGGTTATCA ATAGCCACTC AGTGCGAGAT 
AACTTTAACC GAGTGCTGTG CCCCAAACTA CGCACCTACG TGTGCCCCAT CTGCGGGGCA 
TCTGGGGACT CGGCGCACAC GATTAAGTAC TGCCCCAAGA AGCCGATCAT CACCATGGAG 
GATGCGATCA AGGCGGAATC GTTCCGCCTA GCCAAGAGCA GTTACTACAA GCAACAGATG 
AAGGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262723.1
CDS	264..1412
Translation	MFRSNLEGSGAAADNFSAFHARGGLNILGLQDMYLDTSGANSSATLSPPITPVTPDPSTSAQSTHFPFLADSAATANSLLMQRQYHYHLLLQQQQQLAMAQHQLALAASAAAASASHQQTDEIARSLKIFAQVTTGAAENAAGSMQDVMQEFATNGYASDDLGRMSYGSAPPQVQMPPQQQHQQQQGLHLPLGRNPAQLQTNGGNLMPIPLATHWLNNYREHLNNVWRNMSYIPAAPNTMGLQAQTAATVSTNLGVGMGLGLPVQGEQLRGASNSSNNNNNNNKVYKRYNSKAKEISRHCVFCENNNEPEAVINSHSVRDNFNRVLCPKLRTYVCPICGASGDSAHTIKYCPKKPIITMEDAIKAESFRLAKSSYYKQQMKV

Target ORF information:

RefSeq Version	NM_001275794.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nanos (nos), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275794.1

ORF Insert Sequence:

ATGTTCCGCA GCAACTTGGA GGGCAGTGGC GCAGCAGCAG ATAACTTTTC TGCTTTTCAC 
GCCAGAGGAG GGCTCAACAT TCTGGGCCTG CAGGACATGT ATTTGGATAC CAGTGGGGCC 
AACTCGTCGG CCACTTTGAG TCCGCCCATT ACGCCGGTGA CCCCTGACCC GTCGACGTCT 
GCGCAGTCGA CGCACTTCCC TTTTCTGGCC GACAGCGCAG CCACCGCCAA TTCGCTCCTT 
ATGCAGCGAC AGTACCACTA CCACTTGCTG CTCCAGCAGC AGCAGCAACT GGCCATGGCG 
CAGCACCAAT TGGCGCTGGC TGCATCAGCG GCAGCGGCTA GTGCGAGTCA CCAGCAAACG 
GACGAGATTG CGCGATCCTT GAAAATCTTT GCGCAGGTGA CGACGGGCGC AGCAGAAAAT 
GCGGCTGGCT CGATGCAGGA TGTGATGCAG GAGTTCGCGA CCAATGGCTA TGCCAGCGAT 
GATCTCGGTC GCATGTCCTA CGGGAGTGCT CCGCCACAGG TGCAAATGCC ACCGCAGCAG 
CAGCATCAGC AACAGCAGGG GCTGCACCTG CCACTGGGCC GCAATCCTGC CCAGCTGCAG 
ACCAATGGCG GCAACTTAAT GCCCATTCCA CTCGCCACCC ACTGGCTGAA CAACTACCGG 
GAGCATCTGA ACAACGTGTG GCGAAACATG TCGTATATAC CAGCCGCTCC CAATACAATG 
GGTTTGCAGG CCCAAACAGC GGCCACTGTG TCCACCAATC TCGGCGTGGG AATGGGTCTG 
GGATTGCCCG TGCAGGGCGA ACAGCTGCGC GGAGCTTCCA ATTCCAGTAA CAATAATAAC 
AACAACAACA AGGTGTACAA GCGTTACAAC AGCAAGGCCA AAGAGATCAG CCGCCACTGC 
GTCTTTTGTG AGAATAACAA CGAACCAGAG GCGGTTATCA ATAGCCACTC AGTGCGAGAT 
AACTTTAACC GAGTGCTGTG CCCCAAACTA CGCACCTACG TGTGCCCCAT CTGCGGGGCA 
TCTGGGGACT CGGCGCACAC GATTAAGTAC TGCCCCAAGA AGCCGATCAT CACCATGGAG 
GATGCGATCA AGGCGGAATC GTTCCGCCTA GCCAAGAGCA GTTACTACAA GCAACAGATG 
AAGGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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nos cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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