dnk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	dnk
Entrez Gene ID	42273
Full Name	deoxyribonucleoside kinase
Synonyms	CG5452,DM-dNK,DNK,Dm-dNK,DmdNK,Dmel\CG5452,dNK
General protein information	Preferred Names deoxyribonucleoside kinase Names CG5452 gene product from transcript CG5452-RB 2'-deoxynucleoside kinase CG5452-PA CG5452-PB deoxynucleoside kinase deoxyribonucleoside dnk-PA dnk-PB kinase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 91E2-91E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275793.1	NP_001262722.1	deoxyribonucleoside kinase, isoform B
NM_079675.3	NP_524399.1	deoxyribonucleoside kinase, isoform A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TK2	XP_511013.2
Canis lupus familiaris (dog)	TK2	XP_005620933.1
Rattus norvegicus (Norway rat)	Tk2	NP_001099636.1
Xenopus tropicalis (tropical clawed frog)	tk2	NP_001016888.1
Homo sapiens (human)	TK2	NP_004605.4
	TK2	XP_001083029.1
Drosophila melanogaster (fruit fly)	dnk	NP_001262722.1
Bos taurus (cattle)	TK2	XP_005218881.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001585	XP_003435883.1
Gallus gallus (chicken)	TK2	NP_001026631.1
Danio rerio (zebrafish)	tk2	NP_001002743.1
Mus musculus (house mouse)	Tk2	NP_066356.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We showed that sensitivity of the osteosarcoma cell line to the nucleoside analogs and the efficiency of bystander cell killing were independent of the subcellular localization of Dm-dNK.
Title: Tanshinone IIA enhances bystander cell killing of cancer cells expressing Drosophila melanogaster deoxyribonucleoside kinase in nuclei and mitochondria.

Report synergistic antitumor effect of adenovirus armed with Drosophila melanogaster deoxyribonucleoside kinase and nucleoside analogs for human breast carcinoma.
Title: Synergistic antitumor effect of adenovirus armed with Drosophila melanogaster deoxyribonucleoside kinase and nucleoside analogs for human breast carcinoma in vitro and in vivo.

Data indicate that long term Dm-dNK ( nucleoside kinase from Drosophila melanogaster) expression rescued thymidine kinase 2 (Tk2)-deficient mice without lethal side effects.
Title: Long term expression of Drosophila melanogaster nucleoside kinase in thymidine kinase 2-deficient mice with no lethal effects caused by nucleotide pool imbalances.

Transgene expression of Drosophila melanogaster nucleoside kinase reverses mitochondrial thymidine kinase 2 deficiency
Title: Transgene expression of Drosophila melanogaster nucleoside kinase reverses mitochondrial thymidine kinase 2 deficiency.

Multisubstrate deoxyribonucleoside kinase could be used in a novel therapeutic strategy for treating breast cancer and improving anti-tumor efficacy, as well as for optimizing approaches for suicide gene therapy.
Title: The multisubstrate deoxyribonucleoside kinase of Drosophila melanogaster as a therapeutic suicide gene of breast cancer cells.

The following dnk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dnk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05360	NM_001275793.1 Latest version!	Drosophila melanogaster deoxyribonucleoside kinase (dnk), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa05360	NM_079675.3 Latest version!	Drosophila melanogaster deoxyribonucleoside kinase (dnk), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dnk ( NM_001275793.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262722.1 Drosophila melanogaster deoxyribonucleoside kinase (dnk), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa05360
Clone ID Related Accession (Same CDS sequence)	NM_001275793.1 , NM_079675.3
Accession Version	NM_001275793.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	deoxyribonucleoside kinase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGAGG CAGCATCCTG TGCCCGAAAG GGGACCAAGT ACGCCGAGGG CACCCAGCCC 
TTCACCGTCC TCATCGAGGG CAACATCGGC AGCGGGAAGA CCACGTATTT GAACCACTTC 
GAGAAGTACA AGAACGACAT TTGCCTGCTG ACCGAGCCCG TCGAGAAGTG GCGCAACGTC 
AACGGGGTAA ATCTGCTGGA GCTGATGTAC AAAGATCCCA AGAAGTGGGC CATGCCCTTT 
CAGAGTTATG TCACGCTGAC CATGCTGCAG TCGCACACCG CCCCAACCAA CAAGAAGCTA 
AAAATAATGG AGCGCTCCAT TTTTAGCGCT CGCTATTGCT TCGTGGAGAA CATGCGACGA 
AACGGCTCGC TGGAGCAGGG CATGTACAAT ACGCTGGAGG AGTGGTACAA GTTCATCGAA 
GAGTCCATTC ACGTGCAGGC GGACCTCATC ATATATCTGC GCACCTCGCC GGAGGTGGCG 
TACGAACGCA TCCGGCAGCG GGCTCGTTCT GAGGAGAGCT GCGTGCCGCT TAAGTACCTT 
CAGGAGCTGC ATGAGTTGCA CGAGGACTGG TTGATACACC AGAGACGACC GCAGTCGTGC 
AAGGTCCTAG TCCTCGATGC CGATCTGAAC CTGGAAAACA TTGGCACCGA GTACCAGCGC 
TCGGAGAGCA GCATATTCGA CGCCATCTCA AGTAACCAAC AGCCCTCGCC GGTTCTGGTG 
TCGCCCAGCA AGCGCCAGAG GGTCGCCAGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262722.1
CDS	221..973
Translation	MAEAASCARKGTKYAEGTQPFTVLIEGNIGSGKTTYLNHFEKYKNDICLLTEPVEKWRNVNGVNLLELMYKDPKKWAMPFQSYVTLTMLQSHTAPTNKKLKIMERSIFSARYCFVENMRRNGSLEQGMYNTLEEWYKFIEESIHVQADLIIYLRTSPEVAYERIRQRARSEESCVPLKYLQELHELHEDWLIHQRRPQSCKVLVLDADLNLENIGTEYQRSESSIFDAISSNQQPSPVLVSPSKRQRVAR

Target ORF information:

RefSeq Version	NM_001275793.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster deoxyribonucleoside kinase (dnk), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275793.1

ORF Insert Sequence:

ATGGCGGAGG CAGCATCCTG TGCCCGAAAG GGGACCAAGT ACGCCGAGGG CACCCAGCCC 
TTCACCGTCC TCATCGAGGG CAACATCGGC AGCGGGAAGA CCACGTATTT GAACCACTTC 
GAGAAGTACA AGAACGACAT TTGCCTGCTG ACCGAGCCCG TCGAGAAGTG GCGCAACGTC 
AACGGGGTAA ATCTGCTGGA GCTGATGTAC AAAGATCCCA AGAAGTGGGC CATGCCCTTT 
CAGAGTTATG TCACGCTGAC CATGCTGCAG TCGCACACCG CCCCAACCAA CAAGAAGCTA 
AAAATAATGG AGCGCTCCAT TTTTAGCGCT CGCTATTGCT TCGTGGAGAA CATGCGACGA 
AACGGCTCGC TGGAGCAGGG CATGTACAAT ACGCTGGAGG AGTGGTACAA GTTCATCGAA 
GAGTCCATTC ACGTGCAGGC GGACCTCATC ATATATCTGC GCACCTCGCC GGAGGTGGCG 
TACGAACGCA TCCGGCAGCG GGCTCGTTCT GAGGAGAGCT GCGTGCCGCT TAAGTACCTT 
CAGGAGCTGC ATGAGTTGCA CGAGGACTGG TTGATACACC AGAGACGACC GCAGTCGTGC 
AAGGTCCTAG TCCTCGATGC CGATCTGAAC CTGGAAAACA TTGGCACCGA GTACCAGCGC 
TCGGAGAGCA GCATATTCGA CGCCATCTCA AGTAACCAAC AGCCCTCGCC GGTTCTGGTG 
TCGCCCAGCA AGCGCCAGAG GGTCGCCAGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dnk ( NM_079675.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524399.1 Drosophila melanogaster deoxyribonucleoside kinase (dnk), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa05360
Clone ID Related Accession (Same CDS sequence)	NM_001275793.1 , NM_079675.3
Accession Version	NM_079675.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	deoxyribonucleoside kinase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079675.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCGGAGG CAGCATCCTG TGCCCGAAAG GGGACCAAGT ACGCCGAGGG CACCCAGCCC 
TTCACCGTCC TCATCGAGGG CAACATCGGC AGCGGGAAGA CCACGTATTT GAACCACTTC 
GAGAAGTACA AGAACGACAT TTGCCTGCTG ACCGAGCCCG TCGAGAAGTG GCGCAACGTC 
AACGGGGTAA ATCTGCTGGA GCTGATGTAC AAAGATCCCA AGAAGTGGGC CATGCCCTTT 
CAGAGTTATG TCACGCTGAC CATGCTGCAG TCGCACACCG CCCCAACCAA CAAGAAGCTA 
AAAATAATGG AGCGCTCCAT TTTTAGCGCT CGCTATTGCT TCGTGGAGAA CATGCGACGA 
AACGGCTCGC TGGAGCAGGG CATGTACAAT ACGCTGGAGG AGTGGTACAA GTTCATCGAA 
GAGTCCATTC ACGTGCAGGC GGACCTCATC ATATATCTGC GCACCTCGCC GGAGGTGGCG 
TACGAACGCA TCCGGCAGCG GGCTCGTTCT GAGGAGAGCT GCGTGCCGCT TAAGTACCTT 
CAGGAGCTGC ATGAGTTGCA CGAGGACTGG TTGATACACC AGAGACGACC GCAGTCGTGC 
AAGGTCCTAG TCCTCGATGC CGATCTGAAC CTGGAAAACA TTGGCACCGA GTACCAGCGC 
TCGGAGAGCA GCATATTCGA CGCCATCTCA AGTAACCAAC AGCCCTCGCC GGTTCTGGTG 
TCGCCCAGCA AGCGCCAGAG GGTCGCCAGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524399.1
CDS	149..901
Translation	MAEAASCARKGTKYAEGTQPFTVLIEGNIGSGKTTYLNHFEKYKNDICLLTEPVEKWRNVNGVNLLELMYKDPKKWAMPFQSYVTLTMLQSHTAPTNKKLKIMERSIFSARYCFVENMRRNGSLEQGMYNTLEEWYKFIEESIHVQADLIIYLRTSPEVAYERIRQRARSEESCVPLKYLQELHELHEDWLIHQRRPQSCKVLVLDADLNLENIGTEYQRSESSIFDAISSNQQPSPVLVSPSKRQRVAR

Target ORF information:

RefSeq Version	NM_079675.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster deoxyribonucleoside kinase (dnk), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079675.3

ORF Insert Sequence:

ATGGCGGAGG CAGCATCCTG TGCCCGAAAG GGGACCAAGT ACGCCGAGGG CACCCAGCCC 
TTCACCGTCC TCATCGAGGG CAACATCGGC AGCGGGAAGA CCACGTATTT GAACCACTTC 
GAGAAGTACA AGAACGACAT TTGCCTGCTG ACCGAGCCCG TCGAGAAGTG GCGCAACGTC 
AACGGGGTAA ATCTGCTGGA GCTGATGTAC AAAGATCCCA AGAAGTGGGC CATGCCCTTT 
CAGAGTTATG TCACGCTGAC CATGCTGCAG TCGCACACCG CCCCAACCAA CAAGAAGCTA 
AAAATAATGG AGCGCTCCAT TTTTAGCGCT CGCTATTGCT TCGTGGAGAA CATGCGACGA 
AACGGCTCGC TGGAGCAGGG CATGTACAAT ACGCTGGAGG AGTGGTACAA GTTCATCGAA 
GAGTCCATTC ACGTGCAGGC GGACCTCATC ATATATCTGC GCACCTCGCC GGAGGTGGCG 
TACGAACGCA TCCGGCAGCG GGCTCGTTCT GAGGAGAGCT GCGTGCCGCT TAAGTACCTT 
CAGGAGCTGC ATGAGTTGCA CGAGGACTGG TTGATACACC AGAGACGACC GCAGTCGTGC 
AAGGTCCTAG TCCTCGATGC CGATCTGAAC CTGGAAAACA TTGGCACCGA GTACCAGCGC 
TCGGAGAGCA GCATATTCGA CGCCATCTCA AGTAACCAAC AGCCCTCGCC GGTTCTGGTG 
TCGCCCAGCA AGCGCCAGAG GGTCGCCAGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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dnk cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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