ChAT cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ChAT
Entrez Gene ID	42249
Full Name	Choline acetyltransferase
Synonyms	CG12345,CHAT,CLAT_DROME,CT23399,Cat,ChAt,Cha,ChaT,DChAT,Dmel\CG12345,cha,chat,dChAT,l(3)91Cc
General protein information	Preferred Names Choline acetyltransferase Names CG12345 gene product from transcript CG12345-RA CG12345-PA CG12345-PB ChAT-PA ChAT-PB Choline-acetyltransferase cholinacetyltransferase choline acetyl transferase choline acetyltransferase choline-O-acetyltransferase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 91C1-91C5\|3-64.6 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_206517.2	NP_996239.2	choline acetyltransferase, isoform B
NM_057656.3	NP_477004.5	choline acetyltransferase, isoform A

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Chat	NP_034021.1
Drosophila melanogaster (fruit fly)	Cha	NP_477004.5
Homo sapiens (human)	CHAT	NP_001136405.1
Pan troglodytes (chimpanzee)	CHAT	XP_003339420.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002370	XP_312586.5
Caenorhabditis elegans (roundworm)	cha-1	NP_001023603.1
Xenopus tropicalis (tropical clawed frog)	chat	XP_002933959.1
	CHAT	XP_002805679.1
Bos taurus (cattle)	CHAT	XP_002698999.2
Canis lupus familiaris (dog)	CHAT	XP_005637542.1
Rattus norvegicus (Norway rat)	Chat	NP_001164064.1
Gallus gallus (chicken)	CHAT	NP_989941.1
Danio rerio (zebrafish)	chat	NP_001124191.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ChAT and GFP::ChAT directly bind to the KLP64D tail, which is essential for the GFP::ChAT entry and anterograde flow in axon.
Title: Interaction with a kinesin-2 tail propels choline acetyltransferase flow towards synapse.

The following ChAT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ChAT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05336	NM_057656.3 Latest version!	Drosophila melanogaster choline acetyltransferase, transcript variant A (ChAT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa05337	NM_206517.2 Latest version!	Drosophila melanogaster choline acetyltransferase, transcript variant B (ChAT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ChAT ( NM_057656.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477004.5 Drosophila melanogaster choline acetyltransferase, transcript variant A (ChAT), mRNA.

Price & Availability↑

CloneID	OFa05336
Clone ID Related Accession (Same CDS sequence)	NM_057656.3
Accession Version	NM_057656.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	choline acetyltransferase, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_057656.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

GTGGCGTCCA ACGAGGCCAG CACCAGCGCA GCGGGCAGTG GCCCGGAGTC CGCCGCCCTG 
TTCTCCAAGT TGCGTAGCTT CTCCATTGGC AGCGGGCCGA ACTCGCCGCA GCGCGTCGTC 
TCCAATCTCC GAGGATTCCT CACCCATCGC CTCAGCAACA TCACACCGAG CGATACAGGA 
TGGAAAGACT CGATTCTGTC GATACCAAAG AAATGGCTCT CAACGGCCGA GTCTGTGGAC 
GAGTTTGGAT TCCCTGACAC TCTACCCAAG GTGCCCGTTC CAGCACTGGA TGAAACGATG 
GCCGACTATA TCCGCGCCCT GGAACCGATT ACCACGCCGG CGCAGCTCGA GCGGACCAAG 
GAGCTGATCA GGCAGTTCTC GGCTCCCCAG GGAATCGGAG CGCGGCTGCA TCAGTATCTG 
CTGGACAAGC GTGAGGCGGA GGATAACTGG GCCTATTACT ACTGGCTCAA CGAGATGTAC 
ATGGATATTC GCATCCCTTT GCCGATCAAC TCGAATCCGG GCATGGTGTT CCCGCCGCGT 
CGCTTCAAGA CCGTCCACGA CGTGGCCCAC TTCGCCGCTC GCCTGCTGGA CGGCATTCTG 
AGCCACCGCG AGATGCTGGA CAGTGGGGAG CTGCCGCTGG AGCGGGCCGC CTCGCGGGAA 
AAGAATCAGC CGCTGTGCAT GGCGCAGTAC TACCGCCTGC TGGGCTCCTG TCGTCGGCCT 
GGTGTCAAGC AGGACTCGCA GTTCCTGCCG TCGCGGGAGC GACTGAACGA CGAGGATCGC 
CATGTGGTGG TTATTTGCCG CAACCAAATG TATTGCGTCG TGCTGCAGGC TAGCGATCGT 
GGAAAGTTGT CGGAGAGTGA GATCGCCTCA CAGATCCTCT ATGTGCTCAG TGATGCTCCC 
TGTCTGCCAG CTAAACCAGT GCCGGTGGGT CTGCTGACCG CTGAGCCGAG GAGCACGTGG 
GCACGGGACC GGGAAATGCT TCAGGAGGAC GAACGCAATC AACGCAATCT GGAGCTCATC 
GAGACGGCAC AGGTGGTCCT CTGTCTGGAC GAACCGCTGG CTGGGAACTT TAATGCGCGC 
GGTTTTACGG GTGCCACGCC CACAGTTCAT CGGGCGGGGG ATCGGGACGA GACGAACATG 
GCCCATGAGA TGATCCACGG CGGAGGCAGC GAGTACAATT CCGGAAATCG CTGGTTTGAC 
AAGACCATGC AGCTCATTAT TTGCACCGAT GGAACCTGGG GCCTTTGCTA TGAGCACTCC 
TGTTCCGAAG GCATTGCTGT TGTCCAGCTG CTGGAGAAGA TCTACAAAAA AATCGAGGAG 
CACCCGGACG AGGATAACGG TCTACCGCAA CACCACTTGC CACCACCGGA GCGTCTGGAG 
TGGCATGTGG GTCCGCAATT GCAATTGCGC TTTGCCCAAG CCTCCAAGAG TGTGGACAAA 
TGCATCGATG ACCTGGACTT CTATGTGTAC CGCTACCAGA GTTACGGAAA GACCTTTATC 
AAATCGTGCC AGGTCAGTCC GGATGTGTAC ATTCAACTGG CCCTGCAACT GGCTCACTAC 
AAGCTGTACG GACGTCTGGT GGCCACCTAC GAAAGTGCGT CCACTCGACG ATTTCTGCAC 
GGTCGCGTAG ACTGCATCAG AGCGGCCAGC ACGGAGGCAT TGGAGTGGGC CAAGGCCATG 
TGCCAGGGTG AGGGTGCAAA CGTGCCCCTG GAGAGCGATC GCGAGGATGA GGAGGAGTCG 
CGAAAGGTCA AGTTTAGCAT TTACAGTAAG GATCATCTCC GGGAGCTTTT CCGGTGCGCC 
GTCGCCCGCC AGACTGAGGT GATGGTGAAG AACATCCTGG GCAATGGCAT CGACATCCCG 
CTGCTGGGCC TGCGAGAGGC CAGTATAGAG GTCACCGGCG AGATGCACGA GCTGTTCAAA 
GACGAGTCCT ACATCATCTC GCAGTGCTTC CTGCTCTCCA CCAGTCAGGT GGCCTGCTCT 
ACGGACAGCT TCATGGGATA CGGACCGGTA ACGCCACGTG GTTATGGCTG CTCCTACAAT 
CCGCATCCGG AACAGATCGT CTTCTGCGTC TCGGCCTTCT ACTCCTGTGA GGATACGAGT 
GCCTCGCGCT ACGCCAAATC GCTGCAGGAC TCGCTGGACA TAATGCGTGA TCTACTGCAA 
AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477004.5
CDS	429..2594
Translation	VASNEASTSAAGSGPESAALFSKLRSFSIGSGPNSPQRVVSNLRGFLTHRLSNITPSDTGWKDSILSIPKKWLSTAESVDEFGFPDTLPKVPVPALDETMADYIRALEPITTPAQLERTKELIRQFSAPQGIGARLHQYLLDKREAEDNWAYYYWLNEMYMDIRIPLPINSNPGMVFPPRRFKTVHDVAHFAARLLDGILSHREMLDSGELPLERAASREKNQPLCMAQYYRLLGSCRRPGVKQDSQFLPSRERLNDEDRHVVVICRNQMYCVVLQASDRGKLSESEIASQILYVLSDAPCLPAKPVPVGLLTAEPRSTWARDREMLQEDERNQRNLELIETAQVVLCLDEPLAGNFNARGFTGATPTVHRAGDRDETNMAHEMIHGGGSEYNSGNRWFDKTMQLIICTDGTWGLCYEHSCSEGIAVVQLLEKIYKKIEEHPDEDNGLPQHHLPPPERLEWHVGPQLQLRFAQASKSVDKCIDDLDFYVYRYQSYGKTFIKSCQVSPDVYIQLALQLAHYKLYGRLVATYESASTRRFLHGRVDCIRAASTEALEWAKAMCQGEGANVPLESDREDEEESRKVKFSIYSKDHLRELFRCAVARQTEVMVKNILGNGIDIPLLGLREASIEVTGEMHELFKDESYIISQCFLLSTSQVACSTDSFMGYGPVTPRGYGCSYNPHPEQIVFCVSAFYSCEDTSASRYAKSLQDSLDIMRDLLQN

Target ORF information:

RefSeq Version	NM_057656.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster choline acetyltransferase, transcript variant A (ChAT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057656.3

ORF Insert Sequence:

GTGGCGTCCA ACGAGGCCAG CACCAGCGCA GCGGGCAGTG GCCCGGAGTC CGCCGCCCTG 
TTCTCCAAGT TGCGTAGCTT CTCCATTGGC AGCGGGCCGA ACTCGCCGCA GCGCGTCGTC 
TCCAATCTCC GAGGATTCCT CACCCATCGC CTCAGCAACA TCACACCGAG CGATACAGGA 
TGGAAAGACT CGATTCTGTC GATACCAAAG AAATGGCTCT CAACGGCCGA GTCTGTGGAC 
GAGTTTGGAT TCCCTGACAC TCTACCCAAG GTGCCCGTTC CAGCACTGGA TGAAACGATG 
GCCGACTATA TCCGCGCCCT GGAACCGATT ACCACGCCGG CGCAGCTCGA GCGGACCAAG 
GAGCTGATCA GGCAGTTCTC GGCTCCCCAG GGAATCGGAG CGCGGCTGCA TCAGTATCTG 
CTGGACAAGC GTGAGGCGGA GGATAACTGG GCCTATTACT ACTGGCTCAA CGAGATGTAC 
ATGGATATTC GCATCCCTTT GCCGATCAAC TCGAATCCGG GCATGGTGTT CCCGCCGCGT 
CGCTTCAAGA CCGTCCACGA CGTGGCCCAC TTCGCCGCTC GCCTGCTGGA CGGCATTCTG 
AGCCACCGCG AGATGCTGGA CAGTGGGGAG CTGCCGCTGG AGCGGGCCGC CTCGCGGGAA 
AAGAATCAGC CGCTGTGCAT GGCGCAGTAC TACCGCCTGC TGGGCTCCTG TCGTCGGCCT 
GGTGTCAAGC AGGACTCGCA GTTCCTGCCG TCGCGGGAGC GACTGAACGA CGAGGATCGC 
CATGTGGTGG TTATTTGCCG CAACCAAATG TATTGCGTCG TGCTGCAGGC TAGCGATCGT 
GGAAAGTTGT CGGAGAGTGA GATCGCCTCA CAGATCCTCT ATGTGCTCAG TGATGCTCCC 
TGTCTGCCAG CTAAACCAGT GCCGGTGGGT CTGCTGACCG CTGAGCCGAG GAGCACGTGG 
GCACGGGACC GGGAAATGCT TCAGGAGGAC GAACGCAATC AACGCAATCT GGAGCTCATC 
GAGACGGCAC AGGTGGTCCT CTGTCTGGAC GAACCGCTGG CTGGGAACTT TAATGCGCGC 
GGTTTTACGG GTGCCACGCC CACAGTTCAT CGGGCGGGGG ATCGGGACGA GACGAACATG 
GCCCATGAGA TGATCCACGG CGGAGGCAGC GAGTACAATT CCGGAAATCG CTGGTTTGAC 
AAGACCATGC AGCTCATTAT TTGCACCGAT GGAACCTGGG GCCTTTGCTA TGAGCACTCC 
TGTTCCGAAG GCATTGCTGT TGTCCAGCTG CTGGAGAAGA TCTACAAAAA AATCGAGGAG 
CACCCGGACG AGGATAACGG TCTACCGCAA CACCACTTGC CACCACCGGA GCGTCTGGAG 
TGGCATGTGG GTCCGCAATT GCAATTGCGC TTTGCCCAAG CCTCCAAGAG TGTGGACAAA 
TGCATCGATG ACCTGGACTT CTATGTGTAC CGCTACCAGA GTTACGGAAA GACCTTTATC 
AAATCGTGCC AGGTCAGTCC GGATGTGTAC ATTCAACTGG CCCTGCAACT GGCTCACTAC 
AAGCTGTACG GACGTCTGGT GGCCACCTAC GAAAGTGCGT CCACTCGACG ATTTCTGCAC 
GGTCGCGTAG ACTGCATCAG AGCGGCCAGC ACGGAGGCAT TGGAGTGGGC CAAGGCCATG 
TGCCAGGGTG AGGGTGCAAA CGTGCCCCTG GAGAGCGATC GCGAGGATGA GGAGGAGTCG 
CGAAAGGTCA AGTTTAGCAT TTACAGTAAG GATCATCTCC GGGAGCTTTT CCGGTGCGCC 
GTCGCCCGCC AGACTGAGGT GATGGTGAAG AACATCCTGG GCAATGGCAT CGACATCCCG 
CTGCTGGGCC TGCGAGAGGC CAGTATAGAG GTCACCGGCG AGATGCACGA GCTGTTCAAA 
GACGAGTCCT ACATCATCTC GCAGTGCTTC CTGCTCTCCA CCAGTCAGGT GGCCTGCTCT 
ACGGACAGCT TCATGGGATA CGGACCGGTA ACGCCACGTG GTTATGGCTG CTCCTACAAT 
CCGCATCCGG AACAGATCGT CTTCTGCGTC TCGGCCTTCT ACTCCTGTGA GGATACGAGT 
GCCTCGCGCT ACGCCAAATC GCTGCAGGAC TCGCTGGACA TAATGCGTGA TCTACTGCAA 
AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ChAT ( NM_206517.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996239.2 Drosophila melanogaster choline acetyltransferase, transcript variant B (ChAT), mRNA.

Price & Availability↑

CloneID	OFa05337
Clone ID Related Accession (Same CDS sequence)	NM_206517.2
Accession Version	NM_206517.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	choline acetyltransferase, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_206517.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

GTGGCGTCCA ACGAGGCCAG CACCAGCGCA GCGGGCAGTG GCCCGGAGTC CGCCGCCCTG 
TTCTCCAAGT TGCGTAGCTT CTCCATTGGC AGCGGGCCGA ACTCGCCGCA GCGCGTCGTC 
TCCAATCTCC GAGGATTCCT CACCCATCGC CTCAGCAACA TCACACCGAG CGATACAGGA 
TGGAAAGACT CGATTCTGTC GATACCAAAG AAATGGCTCT CAACGGCCGA GTCTGTGGAC 
GAGTTTGGAT TCCCTGACAC TCTACCCAAG GTGCCCGTTC CAGCACTGGA TGAAACGATG 
GCCGACTATA TCCGCGCCCT GGAACCGATT ACCACGCCGG CGCAGCTCGA GCGGACCAAG 
GAGCTGATCA GGCAGTTCTC GGCTCCCCAG GGAATCGGAG CGCGGCTGCA TCAGTATCTG 
CTGGACAAGC GTGAGGCGGA GGATAACTGG GCCTATTACT ACTGGCTCAA CGAGATGTAC 
ATGGATATTC GCATCCCTTT GCCGATCAAC TCGAATCCGG GCATGGTGTT CCCGCCGCGT 
CGCTTCAAGA CCGTCCACGA CGTGGCCCAC TTCGCCGCTC GCCTGCTGGA CGGCATTCTG 
AGCCACCGCG AGATGCTGGA CAGTGGGGAG CTGCCGCTGG AGCGGGCCGC CTCGCGGGAA 
AAGAATCAGC CGCTGTGCAT GGCGCAGTAC TACCGCCTGC TGGGCTCCTG TCGTCGGCCT 
GGTGTCAAGC AGGACTCGCA GTTCCTGCCG TCGCGGGAGC GACTGAACGA CGAGGATCGC 
CATGTGGTGG TTATTTGCCG CAACCAAATG TATTGCGTCG TGCTGCAGGC TAGCGATCGT 
GGAAAGTTGT CGGAGAGTGA GATCGCCTCA CAGATCCTCT ATGTGCTCAG TGATGCTCCC 
TGTCTGCCAG CTAAACCAGT GCCGGTGGGT CTGCTGACCG CTGAGCCGAG GAGCACGTGG 
GCACGGGACC GGGAAATGCT TCAGGAGGAC GAACGCAATC AACGCAATCT GGAGCTCATC 
GAGACGGCAC AGGTGGTCCT CTGTCTGGAC GAACCGCTGG CTGGGAACTT TAATGCGCGC 
GGTTTTACGG GTGCCACGCC CACAGTTCAT CGGGCGGGGG ATCGGGACGA GACGAACATG 
GCCCATGAGA TGATCCACGG CGGAGGCAGC GAGTACAATT CCGGAAATCG CTGGTTTGAC 
AAGACCATGC AGCTCATTAT TTGCACCGAT GGAACCTGGG GCCTTTGCTA TGAGCACTCC 
TGTTCCGAAG GCATTGCTGT TGTCCAGCTG CTGGAGAAGA TCTACAAAAA AATCGAGGAG 
CACCCGGACG AGGATAACGG TCTACCGCAA CACCACTTGC CACCACCGGA GCGTCTGGAG 
TGGCATGTGG GTCCGCAATT GCAATTGCGC TTTGCCCAAG CCTCCAAGAG TGTGGACAAA 
TGCATCGATG ACCTGGACTT CTATGTGTAC CGCTACCAGA GTTACGGAAA GACCTTTATC 
AAATCGTGCC AGGTCAGTCC GGATGTGTAC ATTCAACTGG CCCTGCAACT GGCTCACTAC 
AAGCTGTACG GACGTCTGGT GGCCACCTAC GAAAGTGCGT CCACTCGACG ATTTCTGCAC 
GGTCGCGTAG ACTGCATCAG AGCGGCCAGC ACGGAGGCAT TGGAGTGGGC CAAGGCCATG 
TGCCAGGGTG AGGGTGCAAA CGTGCCCCTG GAGAGCGATC GCGAGGATGA GGAGGAGTCG 
CGAAAGAAGG ATCATCTCCG GGAGCTTTTC CGGTGCGCCG TCGCCCGCCA GACTGAGGTG 
ATGGTGAAGA ACATCCTGGG CAATGGCATC GACATCCCGC TGCTGGGCCT GCGAGAGGCC 
AGTATAGAGG TCACCGGCGA GATGCACGAG CTGTTCAAAG ACGAGTCCTA CATCATCTCG 
CAGTGCTTCC TGCTCTCCAC CAGTCAGGTG GCCTGCTCTA CGGACAGCTT CATGGGATAC 
GGACCGGTAA CGCCACGTGG TTATGGCTGC TCCTACAATC CGCATCCGGA ACAGATCGTC 
TTCTGCGTCT CGGCCTTCTA CTCCTGTGAG GATACGAGTG CCTCGCGCTA CGCCAAATCG 
CTGCAGGACT CGCTGGACAT AATGCGTGAT CTACTGCAAA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996239.2
CDS	429..2573
Translation	VASNEASTSAAGSGPESAALFSKLRSFSIGSGPNSPQRVVSNLRGFLTHRLSNITPSDTGWKDSILSIPKKWLSTAESVDEFGFPDTLPKVPVPALDETMADYIRALEPITTPAQLERTKELIRQFSAPQGIGARLHQYLLDKREAEDNWAYYYWLNEMYMDIRIPLPINSNPGMVFPPRRFKTVHDVAHFAARLLDGILSHREMLDSGELPLERAASREKNQPLCMAQYYRLLGSCRRPGVKQDSQFLPSRERLNDEDRHVVVICRNQMYCVVLQASDRGKLSESEIASQILYVLSDAPCLPAKPVPVGLLTAEPRSTWARDREMLQEDERNQRNLELIETAQVVLCLDEPLAGNFNARGFTGATPTVHRAGDRDETNMAHEMIHGGGSEYNSGNRWFDKTMQLIICTDGTWGLCYEHSCSEGIAVVQLLEKIYKKIEEHPDEDNGLPQHHLPPPERLEWHVGPQLQLRFAQASKSVDKCIDDLDFYVYRYQSYGKTFIKSCQVSPDVYIQLALQLAHYKLYGRLVATYESASTRRFLHGRVDCIRAASTEALEWAKAMCQGEGANVPLESDREDEEESRKKDHLRELFRCAVARQTEVMVKNILGNGIDIPLLGLREASIEVTGEMHELFKDESYIISQCFLLSTSQVACSTDSFMGYGPVTPRGYGCSYNPHPEQIVFCVSAFYSCEDTSASRYAKSLQDSLDIMRDLLQN

Target ORF information:

RefSeq Version	NM_206517.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster choline acetyltransferase, transcript variant B (ChAT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206517.2

ORF Insert Sequence:

GTGGCGTCCA ACGAGGCCAG CACCAGCGCA GCGGGCAGTG GCCCGGAGTC CGCCGCCCTG 
TTCTCCAAGT TGCGTAGCTT CTCCATTGGC AGCGGGCCGA ACTCGCCGCA GCGCGTCGTC 
TCCAATCTCC GAGGATTCCT CACCCATCGC CTCAGCAACA TCACACCGAG CGATACAGGA 
TGGAAAGACT CGATTCTGTC GATACCAAAG AAATGGCTCT CAACGGCCGA GTCTGTGGAC 
GAGTTTGGAT TCCCTGACAC TCTACCCAAG GTGCCCGTTC CAGCACTGGA TGAAACGATG 
GCCGACTATA TCCGCGCCCT GGAACCGATT ACCACGCCGG CGCAGCTCGA GCGGACCAAG 
GAGCTGATCA GGCAGTTCTC GGCTCCCCAG GGAATCGGAG CGCGGCTGCA TCAGTATCTG 
CTGGACAAGC GTGAGGCGGA GGATAACTGG GCCTATTACT ACTGGCTCAA CGAGATGTAC 
ATGGATATTC GCATCCCTTT GCCGATCAAC TCGAATCCGG GCATGGTGTT CCCGCCGCGT 
CGCTTCAAGA CCGTCCACGA CGTGGCCCAC TTCGCCGCTC GCCTGCTGGA CGGCATTCTG 
AGCCACCGCG AGATGCTGGA CAGTGGGGAG CTGCCGCTGG AGCGGGCCGC CTCGCGGGAA 
AAGAATCAGC CGCTGTGCAT GGCGCAGTAC TACCGCCTGC TGGGCTCCTG TCGTCGGCCT 
GGTGTCAAGC AGGACTCGCA GTTCCTGCCG TCGCGGGAGC GACTGAACGA CGAGGATCGC 
CATGTGGTGG TTATTTGCCG CAACCAAATG TATTGCGTCG TGCTGCAGGC TAGCGATCGT 
GGAAAGTTGT CGGAGAGTGA GATCGCCTCA CAGATCCTCT ATGTGCTCAG TGATGCTCCC 
TGTCTGCCAG CTAAACCAGT GCCGGTGGGT CTGCTGACCG CTGAGCCGAG GAGCACGTGG 
GCACGGGACC GGGAAATGCT TCAGGAGGAC GAACGCAATC AACGCAATCT GGAGCTCATC 
GAGACGGCAC AGGTGGTCCT CTGTCTGGAC GAACCGCTGG CTGGGAACTT TAATGCGCGC 
GGTTTTACGG GTGCCACGCC CACAGTTCAT CGGGCGGGGG ATCGGGACGA GACGAACATG 
GCCCATGAGA TGATCCACGG CGGAGGCAGC GAGTACAATT CCGGAAATCG CTGGTTTGAC 
AAGACCATGC AGCTCATTAT TTGCACCGAT GGAACCTGGG GCCTTTGCTA TGAGCACTCC 
TGTTCCGAAG GCATTGCTGT TGTCCAGCTG CTGGAGAAGA TCTACAAAAA AATCGAGGAG 
CACCCGGACG AGGATAACGG TCTACCGCAA CACCACTTGC CACCACCGGA GCGTCTGGAG 
TGGCATGTGG GTCCGCAATT GCAATTGCGC TTTGCCCAAG CCTCCAAGAG TGTGGACAAA 
TGCATCGATG ACCTGGACTT CTATGTGTAC CGCTACCAGA GTTACGGAAA GACCTTTATC 
AAATCGTGCC AGGTCAGTCC GGATGTGTAC ATTCAACTGG CCCTGCAACT GGCTCACTAC 
AAGCTGTACG GACGTCTGGT GGCCACCTAC GAAAGTGCGT CCACTCGACG ATTTCTGCAC 
GGTCGCGTAG ACTGCATCAG AGCGGCCAGC ACGGAGGCAT TGGAGTGGGC CAAGGCCATG 
TGCCAGGGTG AGGGTGCAAA CGTGCCCCTG GAGAGCGATC GCGAGGATGA GGAGGAGTCG 
CGAAAGAAGG ATCATCTCCG GGAGCTTTTC CGGTGCGCCG TCGCCCGCCA GACTGAGGTG 
ATGGTGAAGA ACATCCTGGG CAATGGCATC GACATCCCGC TGCTGGGCCT GCGAGAGGCC 
AGTATAGAGG TCACCGGCGA GATGCACGAG CTGTTCAAAG ACGAGTCCTA CATCATCTCG 
CAGTGCTTCC TGCTCTCCAC CAGTCAGGTG GCCTGCTCTA CGGACAGCTT CATGGGATAC 
GGACCGGTAA CGCCACGTGG TTATGGCTGC TCCTACAATC CGCATCCGGA ACAGATCGTC 
TTCTGCGTCT CGGCCTTCTA CTCCTGTGAG GATACGAGTG CCTCGCGCTA CGCCAAATCG 
CTGCAGGACT CGCTGGACAT AATGCGTGAT CTACTGCAAA ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
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The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
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ChAT cDNA ORF clone, Drosophila melanogaster(fruit fly)

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ORF Insert Sequence:

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ORF Insert Sequence:

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