CG31224 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG31224
Entrez Gene ID	42237
Full Name	CG31224 gene product from transcript CG31224-RB
Synonyms	CG17796,CG7696,Dmel\CG31224,l(3)c00083
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 91B8-91B8

mRNA and Protein(s)

mRNA	Protein	Name
NM_169826.2	NP_732361.1	uncharacterized protein, isoform A
NM_001300467.1	NP_001287396.1	uncharacterized protein, isoform B
NM_001300468.1	NP_001287397.1	uncharacterized protein, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG31224 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG31224 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05326	NM_169826.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG31224), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa05326	NM_001300467.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG31224), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa05326	NM_001300468.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG31224), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG31224 ( NM_169826.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732361.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG31224), mRNA.

Price & Availability↑

CloneID	OFa05326
Clone ID Related Accession (Same CDS sequence)	NM_169826.2 , NM_001300467.1 , NM_001300468.1
Accession Version	NM_169826.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169826.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCGACA ACAGTGTCCA ATGCCCCGTG TGCACATTGT ACCTGCATGC GGGCATGAAT 
CTCTCCGATC ACTTGGAGAC CCATCCCAAG GAGCAGGTGA TCAAGGCGCT GGTGCAGATG 
ACGATCGTTG GGAACGGTGG CAACAGCATG GGCAGCGTTC TCGCTGCCGC CATGTTGACA 
GGAGGATCTG GAGGAGGTGG ATCCAGCGGC GGCGAAGCCA GTGCTGATGA GAAACCGGCC 
GTCTTATCAC CCCCTTCTGG CGGCGATGTC AAACCAGTCG TGGTCAATAC CATATCAGCA 
GCAACAGCGC CTGTGCTGCC ACCAGCACCA CCACCGCTAT CCTCCTCCCT CGTGGCGGCC 
TGCTCCAACG GAGCCGCCTC TTCGACAACC AGCTCCACAC CCACCTCCAC CTCAGCAAGC 
AACAGCAGCA GCCTCAGCAG CAGCAAACCG AGCAATCCAC CGATGAAAGC ACTGAAATGC 
TCACCGCCCA CAACTGAGGC GCGAGCCAGC ACATCCGCAC ATCCCACCAC GAGCAGCGTG 
TCCACTTTGT ACCAGCCAGC GCAGGTCACT CCGGATTATC GCTTCAACCA GCAGCAGCAG 
CAGCCGCAGA CGTCGGGCTT TGGGCGTGGT GTCACCACCT CAACAGCCGC GCTGGTGATT 
CCGCAGGTGC CTCAGCAGCA CTTCCTCGCC AGCCATCAAC AGCAGGCTCA CTTTCCACAC 
CAGCAGCAGT CCCCGCAGCA ACAGCAGCAA CATTCGCAGC ATCAGCCTGC TCTGAAGTTC 
AGCTACGCCA CAGCTTTGCC CCCACCTCCG CCGTTGCAGC TATTTACGCA GCAACAGCAG 
CAGCAACAGC AGACGGTTGT TCCACCGCAG ACCCAGCAGC AATCGCACCA GAAACCACCT 
CCCGCCTATG GAGCCGCCAT TAGTCAAATA CGATCTCAGC ACAACCAAAA CAAACAGCAG 
CCACAGTCCC AAAGCGTGGC CATGCAACCC AACCTAGCCC TGGCACCAAC GACGACATCA 
GCGGCTTCAA CTTCACACGC AACTGCAGCT GTTAAGCAGC CCAGTAAACC TCATACGGAA 
GTATTCCTGA ATCCCCCACC ACCGCCATCG GCACAGCAAC AGTTGCAGAT CCACAGTTTA 
GTGCAAAATC AAGGTCAACA CCATCAGCAT TCTCACCCGC AGCAGTCGCA GCCGAGCCAG 
CAGAGTCACC AACAGCCACC GAGCTTGCAG ACCTACGGAT TAGGGGTCAG CAATAGCTGC 
AGTTCCTCCA ACAACCCCTC GACCTCGGGA TCTGGTCATC GTGCGAATCG TCAGACAAAC 
TCACCGTTTG GAGCACTGCT TAATGCTGCA GCGGCAGTGG CCTGCGCATC GCCGGCCTGT 
CCCACCACCT CATCTTCGGT ATTGAGGTTT GCAGAATCAC CGGTGGCACA CTACCTTGAG 
CGGGAAAATG GTGACTTCAT TGTCCAGGAG ACACCGAAGC ATATTGTAGA GTGTGTGGAG 
AAGGAAGACG GCGAGTTTTC CGTGATCGAA CGCATTTATC AATCCCCGCC GAGTGTTCTG 
CACATACACG ATGATGATGA GGATGACGAG GATGAAGAGG AGGAGGACGA GCATGATGCT 
CCGCCCGAGG AAGAAGACGG GGACGATGAG CGGGAGGAGA ACAGCCACAG CCGTAATATG 
AGCAAGACAA CGAAAAAGGC CCGTAAGACG AAGCCGAAGC GAGGCTCCAA GCGCATGTCG 
GACGAGGAGG AGTTCATGAG CCTTAGCAGC GGATGTGAGA GTGATTTGGA AAAGGAGGGT 
AAACAGGAGC AGCTAAATGC AGCGGCATCA CTACCACCTC CATTGTGCAC GGCTCCTTCT 
ACAAGTGCTG CGGCTGGAGC TTCCACCTCC AATCTGGCGG ACAATCATTC CAATTCCGCG 
TCCAACTCTG CCTCCAATTC GAACTCCACC GCCACTACAA AGTCCAAGAA GAATCGAATT 
ACCGTACTCA GCGATGTTCC GCTCAATATG AATGAGTACC TCGATTTGAT GGGCAACATC 
GTAGCCTCCA GTCGCATTGG GGCAAGTCGA CCGTTTGCCG CTGTGGCGCC CATACCACTC 
GTCAAGGTGG AGAAGGAAGA ACCTCTGGAT GATTACGACA ACGTGGCAAT GGCCGCTAAT 
GAAGAGCTGG AGCAAAAGCC CAGCCTTGAG CATGGCACCA CTAGCGTCAT TCGAATGGCA 
AGCACGGCTG CAGCATCGAC TTCTGCAACG GCGACCGAAA CATCTGCCGA GGATCTCACT 
CAGCCGCCGC CCATGAAAGT CGAGGTTGAG GCGACCACCA CATTGTTGCC GCAACGCTTT 
TCAACTCCCG CCCAGCATCG CGGGCCTAAG AAATTAGTCA TCAAACCAAA ACAAACAGCA 
CCAGCGACAG CGACGACAAC AAAAAAGACT GACACCCCAT CTTCCCCCAG CAACAACCCA 
TCCATATCAA GAGATCTGGT GCAAGTAAAG AGTGAGACAC TGGAGGCGCC AGCGACGAGC 
AGTAGCAGTA GCATTCTGGA GAAGCACCTA ACCACCAGCC GAAAACTTTT GCATCATCAC 
TCGGCGGTGA ACGATGACGA TGCCCGGGTG CTGCTGGAGT TTGCGAACTC GAAGCAGCCG 
CCGCCACTGG CCGCGTCGAG CACAACGTTT GTGGTTAATG CCAGTGTAGG CTTTCCCTCG 
GCTGGCTCAG TTTTCGCCAG CAGCAGTACC AGCTGTTCCA AAGCACCGGC CAAGCCGGGA 
GAGGAGTACA TCCTGCCGGA CAACAACATG GACGTGGATG AGATTGTGAT ATCCTCGTCG 
TCTTCCTACA CCTCCTCTGC AGCCCAGGTG CAGAACACGT TACAGCCGCC GGACTTTAAC 
TTTCTGTACC ACCAGGCCAC TACAACGGCC ACGACGGCCA CATACTTCAA TCCCTTTTCC 
AGGCAGCAGC AGCATCATCA TCAGCAGCAG CACGGCGTCA GCGATGCCAC CAATGCGGCT 
ACTTTGGCTT CGTCGTCCAG TAATTCGTTG GACCACGAGT CGATGAATGC TACATTCCGG 
GTGGCCAAGA CCCAATCGCT GCAGTCATGG TATCAGCAAG AGCCGGATCA CGATCCACAG 
AATCAGGGTG GTGCGGAATC GGGAACTGTC ACTCCCTCCA ATCAGAGCCA GGATGTGGTT 
AGCCACCAGC CAACAAACTT TAACGCCGCC CTAGCGGCCG TCGATTGCTG TAGCGTTGCC 
TTGGACGAGG ACGCAAAATA TCTTGATCTA GACGCCTGCA AGCGTGAGCA GCTGAGCAGC 
AGTAGCAATC TGCCCTCCGC TTCCGCGTCC ACAACGTCGT CGTCGTTCGC TGGCGCTGGC 
ACGGAGAGCA GTTTGGTGGG TGGTCTGAAT ATCCGAACCG ATGAAAAGAT GCCCGCCAAG 
GGTGAAATTT CCGAACAGGA AAGCAACTGC GACATCGAGA ACTCCTGGAG TGAGTCGGTT 
TATGGGGATA TTTCGCAGCG GTACTTCAGA AACCAGTTTT CTGGCGGCTA CCACCCTGAT 
TGGCGACAGG ACTATTATCC GGCTCAAGAT CTCAGCGCTC AGCTGAGAGA GCATAAACGT 
TTTGACTTTA ATGCTGTCGA TGATGAGGCT TCATCCAGCT CGAGAAGGAG CCATCTTACA 
GATCTCCCGA CAGCGGCCAC CTCCTCCGCA TGTACGTCAT CAGCTATGCT GGCGGGTCCA 
CCAATAGCTT CGACATCACG GGCTGCTGCT GAGGCGGCAG CGGAGGCGGC TGCTCTTGCC 
CAGCGGAAAC CGCAGCGCCG CAAGCTCTAC AAGTGTCCTC ACTGCGAGGC GATCTTCGAA 
AAGCTTAAGG AGCGTAATGC GCACATGATT GGCGAGCACA ACTACGTACG GCAAAATCGC 
CGTCTAATTT GCACTCAGCC GCAAATCAAT ACTTCGATGT TAACGCTACA GCAACAGATG 
ATAGTGCAGT CAGGTGAGGT TGAGGATTCC AAGGATGGCA TTGTGAAGAT AAAACAGGAG 
CAGTGCCAGG AAAAGCAGGA TGTTGAGCAA ATGCACGACC ATTCCGAACT ATTGGCTGAT 
GTCAAGGACT CTGAGCTGAT GGGCATCGGC GGTGATGGTG ACGGTGATGT AGATGACGAC 
ATTAAGCCAC CCAACCAGCT AGATGAAAAG CCCACGCTGC CGCCATTAGC CATGACGACC 
CCAGCGGCTA AATTAGCAGC TTTGTATCGA ATGCTCATCG CCTACAACGA ATCCAAGCTC 
GGACAGGAGC GCAACAATCT TAGCGAGCTG GAGCAGAAGG CTATGGAAAA ATCCATATTT 
CTCTGCTATG TCTGTCGTAC CGACTTCCCG TCCGTAAAGC TCTATGACGC CCATCTCACC 
GAGCATCCCG CTGAATGCTT TACGTGCGGC AAGAAGTTCT ACCGTTGGAA GAACTTCTCA 
TTGCACTTGA AGCGGCACTT GGGCTGGAAG GAGTTCGGAT GCTATGTCTG CGACAAGAAG 
TTCGTAGTGC GCAGCGCCTT GGTCGAACAC ATGCGGATGC ACACGGGGCA AACGCCACTC 
AAGTGCAAGA TATGCGGTAA AAAGTTCAAG AGATACTCGA ATCTGACGCA ACACCGCAAA 
CGCCACACCA AAATGATGGT GCGCAAGAAG GAATACGTGT GTCACTGCGG CGAAGTCTTG 
CCCTCGAAGG CTCGCTTCCT GTGGCACAAG GAAACGCACG ACCTGAAGCC GAAGTGCTGC 
CCGTACTGCT GCGATCGGTT TGTGCACGCC AACTCGCTAC GTCGACACAT ACGGTTGGCC 
CACTCTGATA AGTTTGACTA TGCCGAACCT ATGGAATGCC CTATGTGTAA GCAAATCTTC 
GCGAAGACGT CCATCAAGGC GCACATGGCG ACGCACTCAA CGGAGCCCCA ATACGATTGC 
GCCATTTGTA GCAAGAGCTT CTCCACCAAA TGGAATCTCA AGATCCACTC GTGGGTACAC 
GCCAACCGGA CGGCCAAGCC CTTTAAGTGC GAATACTGCC CAAAGGCGTT TGTGCGAGAG 
CTGGACTTCA AGAACCACAT CAACGCGCAC AAACAGATCA AACCGTACAC GTGCGAGTAC 
TGCGGCTGCA AGTTCATCCG CAAGTACAAT TACATGCGAC ACCGGCGCGA ACATCATGGC 
ACCAAGAAGT TCACCTGCGA CCAATGCGAT AAGTCTTTCC ATCGGCATTA CTATCTGATA 
GAGCACCGGC GAATGCATAC CGGGGAGCGT CCGTTCACGT GTACCATCTG CGGCAAGAGC 
TCCACAACGA AGACCAACCA CAACAAGCAT CTGAAGATCC ACCATTCGCG CGATCCGTTC 
ACGGTGGAGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732361.1
CDS	753..6107
Translation	MVDNSVQCPVCTLYLHAGMNLSDHLETHPKEQVIKALVQMTIVGNGGNSMGSVLAAAMLTGGSGGGGSSGGEASADEKPAVLSPPSGGDVKPVVVNTISAATAPVLPPAPPPLSSSLVAACSNGAASSTTSSTPTSTSASNSSSLSSSKPSNPPMKALKCSPPTTEARASTSAHPTTSSVSTLYQPAQVTPDYRFNQQQQQPQTSGFGRGVTTSTAALVIPQVPQQHFLASHQQQAHFPHQQQSPQQQQQHSQHQPALKFSYATALPPPPPLQLFTQQQQQQQQTVVPPQTQQQSHQKPPPAYGAAISQIRSQHNQNKQQPQSQSVAMQPNLALAPTTTSAASTSHATAAVKQPSKPHTEVFLNPPPPPSAQQQLQIHSLVQNQGQHHQHSHPQQSQPSQQSHQQPPSLQTYGLGVSNSCSSSNNPSTSGSGHRANRQTNSPFGALLNAAAAVACASPACPTTSSSVLRFAESPVAHYLERENGDFIVQETPKHIVECVEKEDGEFSVIERIYQSPPSVLHIHDDDEDDEDEEEEDEHDAPPEEEDGDDEREENSHSRNMSKTTKKARKTKPKRGSKRMSDEEEFMSLSSGCESDLEKEGKQEQLNAAASLPPPLCTAPSTSAAAGASTSNLADNHSNSASNSASNSNSTATTKSKKNRITVLSDVPLNMNEYLDLMGNIVASSRIGASRPFAAVAPIPLVKVEKEEPLDDYDNVAMAANEELEQKPSLEHGTTSVIRMASTAAASTSATATETSAEDLTQPPPMKVEVEATTTLLPQRFSTPAQHRGPKKLVIKPKQTAPATATTTKKTDTPSSPSNNPSISRDLVQVKSETLEAPATSSSSSILEKHLTTSRKLLHHHSAVNDDDARVLLEFANSKQPPPLAASSTTFVVNASVGFPSAGSVFASSSTSCSKAPAKPGEEYILPDNNMDVDEIVISSSSSYTSSAAQVQNTLQPPDFNFLYHQATTTATTATYFNPFSRQQQHHHQQQHGVSDATNAATLASSSSNSLDHESMNATFRVAKTQSLQSWYQQEPDHDPQNQGGAESGTVTPSNQSQDVVSHQPTNFNAALAAVDCCSVALDEDAKYLDLDACKREQLSSSSNLPSASASTTSSSFAGAGTESSLVGGLNIRTDEKMPAKGEISEQESNCDIENSWSESVYGDISQRYFRNQFSGGYHPDWRQDYYPAQDLSAQLREHKRFDFNAVDDEASSSSRRSHLTDLPTAATSSACTSSAMLAGPPIASTSRAAAEAAAEAAALAQRKPQRRKLYKCPHCEAIFEKLKERNAHMIGEHNYVRQNRRLICTQPQINTSMLTLQQQMIVQSGEVEDSKDGIVKIKQEQCQEKQDVEQMHDHSELLADVKDSELMGIGGDGDGDVDDDIKPPNQLDEKPTLPPLAMTTPAAKLAALYRMLIAYNESKLGQERNNLSELEQKAMEKSIFLCYVCRTDFPSVKLYDAHLTEHPAECFTCGKKFYRWKNFSLHLKRHLGWKEFGCYVCDKKFVVRSALVEHMRMHTGQTPLKCKICGKKFKRYSNLTQHRKRHTKMMVRKKEYVCHCGEVLPSKARFLWHKETHDLKPKCCPYCCDRFVHANSLRRHIRLAHSDKFDYAEPMECPMCKQIFAKTSIKAHMATHSTEPQYDCAICSKSFSTKWNLKIHSWVHANRTAKPFKCEYCPKAFVRELDFKNHINAHKQIKPYTCEYCGCKFIRKYNYMRHRREHHGTKKFTCDQCDKSFHRHYYLIEHRRMHTGERPFTCTICGKSSTTKTNHNKHLKIHHSRDPFTVEA

Target ORF information:

RefSeq Version	NM_169826.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG31224), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169826.2

ORF Insert Sequence:

ATGGTCGACA ACAGTGTCCA ATGCCCCGTG TGCACATTGT ACCTGCATGC GGGCATGAAT 
CTCTCCGATC ACTTGGAGAC CCATCCCAAG GAGCAGGTGA TCAAGGCGCT GGTGCAGATG 
ACGATCGTTG GGAACGGTGG CAACAGCATG GGCAGCGTTC TCGCTGCCGC CATGTTGACA 
GGAGGATCTG GAGGAGGTGG ATCCAGCGGC GGCGAAGCCA GTGCTGATGA GAAACCGGCC 
GTCTTATCAC CCCCTTCTGG CGGCGATGTC AAACCAGTCG TGGTCAATAC CATATCAGCA 
GCAACAGCGC CTGTGCTGCC ACCAGCACCA CCACCGCTAT CCTCCTCCCT CGTGGCGGCC 
TGCTCCAACG GAGCCGCCTC TTCGACAACC AGCTCCACAC CCACCTCCAC CTCAGCAAGC 
AACAGCAGCA GCCTCAGCAG CAGCAAACCG AGCAATCCAC CGATGAAAGC ACTGAAATGC 
TCACCGCCCA CAACTGAGGC GCGAGCCAGC ACATCCGCAC ATCCCACCAC GAGCAGCGTG 
TCCACTTTGT ACCAGCCAGC GCAGGTCACT CCGGATTATC GCTTCAACCA GCAGCAGCAG 
CAGCCGCAGA CGTCGGGCTT TGGGCGTGGT GTCACCACCT CAACAGCCGC GCTGGTGATT 
CCGCAGGTGC CTCAGCAGCA CTTCCTCGCC AGCCATCAAC AGCAGGCTCA CTTTCCACAC 
CAGCAGCAGT CCCCGCAGCA ACAGCAGCAA CATTCGCAGC ATCAGCCTGC TCTGAAGTTC 
AGCTACGCCA CAGCTTTGCC CCCACCTCCG CCGTTGCAGC TATTTACGCA GCAACAGCAG 
CAGCAACAGC AGACGGTTGT TCCACCGCAG ACCCAGCAGC AATCGCACCA GAAACCACCT 
CCCGCCTATG GAGCCGCCAT TAGTCAAATA CGATCTCAGC ACAACCAAAA CAAACAGCAG 
CCACAGTCCC AAAGCGTGGC CATGCAACCC AACCTAGCCC TGGCACCAAC GACGACATCA 
GCGGCTTCAA CTTCACACGC AACTGCAGCT GTTAAGCAGC CCAGTAAACC TCATACGGAA 
GTATTCCTGA ATCCCCCACC ACCGCCATCG GCACAGCAAC AGTTGCAGAT CCACAGTTTA 
GTGCAAAATC AAGGTCAACA CCATCAGCAT TCTCACCCGC AGCAGTCGCA GCCGAGCCAG 
CAGAGTCACC AACAGCCACC GAGCTTGCAG ACCTACGGAT TAGGGGTCAG CAATAGCTGC 
AGTTCCTCCA ACAACCCCTC GACCTCGGGA TCTGGTCATC GTGCGAATCG TCAGACAAAC 
TCACCGTTTG GAGCACTGCT TAATGCTGCA GCGGCAGTGG CCTGCGCATC GCCGGCCTGT 
CCCACCACCT CATCTTCGGT ATTGAGGTTT GCAGAATCAC CGGTGGCACA CTACCTTGAG 
CGGGAAAATG GTGACTTCAT TGTCCAGGAG ACACCGAAGC ATATTGTAGA GTGTGTGGAG 
AAGGAAGACG GCGAGTTTTC CGTGATCGAA CGCATTTATC AATCCCCGCC GAGTGTTCTG 
CACATACACG ATGATGATGA GGATGACGAG GATGAAGAGG AGGAGGACGA GCATGATGCT 
CCGCCCGAGG AAGAAGACGG GGACGATGAG CGGGAGGAGA ACAGCCACAG CCGTAATATG 
AGCAAGACAA CGAAAAAGGC CCGTAAGACG AAGCCGAAGC GAGGCTCCAA GCGCATGTCG 
GACGAGGAGG AGTTCATGAG CCTTAGCAGC GGATGTGAGA GTGATTTGGA AAAGGAGGGT 
AAACAGGAGC AGCTAAATGC AGCGGCATCA CTACCACCTC CATTGTGCAC GGCTCCTTCT 
ACAAGTGCTG CGGCTGGAGC TTCCACCTCC AATCTGGCGG ACAATCATTC CAATTCCGCG 
TCCAACTCTG CCTCCAATTC GAACTCCACC GCCACTACAA AGTCCAAGAA GAATCGAATT 
ACCGTACTCA GCGATGTTCC GCTCAATATG AATGAGTACC TCGATTTGAT GGGCAACATC 
GTAGCCTCCA GTCGCATTGG GGCAAGTCGA CCGTTTGCCG CTGTGGCGCC CATACCACTC 
GTCAAGGTGG AGAAGGAAGA ACCTCTGGAT GATTACGACA ACGTGGCAAT GGCCGCTAAT 
GAAGAGCTGG AGCAAAAGCC CAGCCTTGAG CATGGCACCA CTAGCGTCAT TCGAATGGCA 
AGCACGGCTG CAGCATCGAC TTCTGCAACG GCGACCGAAA CATCTGCCGA GGATCTCACT 
CAGCCGCCGC CCATGAAAGT CGAGGTTGAG GCGACCACCA CATTGTTGCC GCAACGCTTT 
TCAACTCCCG CCCAGCATCG CGGGCCTAAG AAATTAGTCA TCAAACCAAA ACAAACAGCA 
CCAGCGACAG CGACGACAAC AAAAAAGACT GACACCCCAT CTTCCCCCAG CAACAACCCA 
TCCATATCAA GAGATCTGGT GCAAGTAAAG AGTGAGACAC TGGAGGCGCC AGCGACGAGC 
AGTAGCAGTA GCATTCTGGA GAAGCACCTA ACCACCAGCC GAAAACTTTT GCATCATCAC 
TCGGCGGTGA ACGATGACGA TGCCCGGGTG CTGCTGGAGT TTGCGAACTC GAAGCAGCCG 
CCGCCACTGG CCGCGTCGAG CACAACGTTT GTGGTTAATG CCAGTGTAGG CTTTCCCTCG 
GCTGGCTCAG TTTTCGCCAG CAGCAGTACC AGCTGTTCCA AAGCACCGGC CAAGCCGGGA 
GAGGAGTACA TCCTGCCGGA CAACAACATG GACGTGGATG AGATTGTGAT ATCCTCGTCG 
TCTTCCTACA CCTCCTCTGC AGCCCAGGTG CAGAACACGT TACAGCCGCC GGACTTTAAC 
TTTCTGTACC ACCAGGCCAC TACAACGGCC ACGACGGCCA CATACTTCAA TCCCTTTTCC 
AGGCAGCAGC AGCATCATCA TCAGCAGCAG CACGGCGTCA GCGATGCCAC CAATGCGGCT 
ACTTTGGCTT CGTCGTCCAG TAATTCGTTG GACCACGAGT CGATGAATGC TACATTCCGG 
GTGGCCAAGA CCCAATCGCT GCAGTCATGG TATCAGCAAG AGCCGGATCA CGATCCACAG 
AATCAGGGTG GTGCGGAATC GGGAACTGTC ACTCCCTCCA ATCAGAGCCA GGATGTGGTT 
AGCCACCAGC CAACAAACTT TAACGCCGCC CTAGCGGCCG TCGATTGCTG TAGCGTTGCC 
TTGGACGAGG ACGCAAAATA TCTTGATCTA GACGCCTGCA AGCGTGAGCA GCTGAGCAGC 
AGTAGCAATC TGCCCTCCGC TTCCGCGTCC ACAACGTCGT CGTCGTTCGC TGGCGCTGGC 
ACGGAGAGCA GTTTGGTGGG TGGTCTGAAT ATCCGAACCG ATGAAAAGAT GCCCGCCAAG 
GGTGAAATTT CCGAACAGGA AAGCAACTGC GACATCGAGA ACTCCTGGAG TGAGTCGGTT 
TATGGGGATA TTTCGCAGCG GTACTTCAGA AACCAGTTTT CTGGCGGCTA CCACCCTGAT 
TGGCGACAGG ACTATTATCC GGCTCAAGAT CTCAGCGCTC AGCTGAGAGA GCATAAACGT 
TTTGACTTTA ATGCTGTCGA TGATGAGGCT TCATCCAGCT CGAGAAGGAG CCATCTTACA 
GATCTCCCGA CAGCGGCCAC CTCCTCCGCA TGTACGTCAT CAGCTATGCT GGCGGGTCCA 
CCAATAGCTT CGACATCACG GGCTGCTGCT GAGGCGGCAG CGGAGGCGGC TGCTCTTGCC 
CAGCGGAAAC CGCAGCGCCG CAAGCTCTAC AAGTGTCCTC ACTGCGAGGC GATCTTCGAA 
AAGCTTAAGG AGCGTAATGC GCACATGATT GGCGAGCACA ACTACGTACG GCAAAATCGC 
CGTCTAATTT GCACTCAGCC GCAAATCAAT ACTTCGATGT TAACGCTACA GCAACAGATG 
ATAGTGCAGT CAGGTGAGGT TGAGGATTCC AAGGATGGCA TTGTGAAGAT AAAACAGGAG 
CAGTGCCAGG AAAAGCAGGA TGTTGAGCAA ATGCACGACC ATTCCGAACT ATTGGCTGAT 
GTCAAGGACT CTGAGCTGAT GGGCATCGGC GGTGATGGTG ACGGTGATGT AGATGACGAC 
ATTAAGCCAC CCAACCAGCT AGATGAAAAG CCCACGCTGC CGCCATTAGC CATGACGACC 
CCAGCGGCTA AATTAGCAGC TTTGTATCGA ATGCTCATCG CCTACAACGA ATCCAAGCTC 
GGACAGGAGC GCAACAATCT TAGCGAGCTG GAGCAGAAGG CTATGGAAAA ATCCATATTT 
CTCTGCTATG TCTGTCGTAC CGACTTCCCG TCCGTAAAGC TCTATGACGC CCATCTCACC 
GAGCATCCCG CTGAATGCTT TACGTGCGGC AAGAAGTTCT ACCGTTGGAA GAACTTCTCA 
TTGCACTTGA AGCGGCACTT GGGCTGGAAG GAGTTCGGAT GCTATGTCTG CGACAAGAAG 
TTCGTAGTGC GCAGCGCCTT GGTCGAACAC ATGCGGATGC ACACGGGGCA AACGCCACTC 
AAGTGCAAGA TATGCGGTAA AAAGTTCAAG AGATACTCGA ATCTGACGCA ACACCGCAAA 
CGCCACACCA AAATGATGGT GCGCAAGAAG GAATACGTGT GTCACTGCGG CGAAGTCTTG 
CCCTCGAAGG CTCGCTTCCT GTGGCACAAG GAAACGCACG ACCTGAAGCC GAAGTGCTGC 
CCGTACTGCT GCGATCGGTT TGTGCACGCC AACTCGCTAC GTCGACACAT ACGGTTGGCC 
CACTCTGATA AGTTTGACTA TGCCGAACCT ATGGAATGCC CTATGTGTAA GCAAATCTTC 
GCGAAGACGT CCATCAAGGC GCACATGGCG ACGCACTCAA CGGAGCCCCA ATACGATTGC 
GCCATTTGTA GCAAGAGCTT CTCCACCAAA TGGAATCTCA AGATCCACTC GTGGGTACAC 
GCCAACCGGA CGGCCAAGCC CTTTAAGTGC GAATACTGCC CAAAGGCGTT TGTGCGAGAG 
CTGGACTTCA AGAACCACAT CAACGCGCAC AAACAGATCA AACCGTACAC GTGCGAGTAC 
TGCGGCTGCA AGTTCATCCG CAAGTACAAT TACATGCGAC ACCGGCGCGA ACATCATGGC 
ACCAAGAAGT TCACCTGCGA CCAATGCGAT AAGTCTTTCC ATCGGCATTA CTATCTGATA 
GAGCACCGGC GAATGCATAC CGGGGAGCGT CCGTTCACGT GTACCATCTG CGGCAAGAGC 
TCCACAACGA AGACCAACCA CAACAAGCAT CTGAAGATCC ACCATTCGCG CGATCCGTTC 
ACGGTGGAGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG31224 ( NM_001300467.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287396.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG31224), mRNA.

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CloneID	OFa05326
Clone ID Related Accession (Same CDS sequence)	NM_169826.2 , NM_001300467.1 , NM_001300468.1
Accession Version	NM_001300467.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCGACA ACAGTGTCCA ATGCCCCGTG TGCACATTGT ACCTGCATGC GGGCATGAAT 
CTCTCCGATC ACTTGGAGAC CCATCCCAAG GAGCAGGTGA TCAAGGCGCT GGTGCAGATG 
ACGATCGTTG GGAACGGTGG CAACAGCATG GGCAGCGTTC TCGCTGCCGC CATGTTGACA 
GGAGGATCTG GAGGAGGTGG ATCCAGCGGC GGCGAAGCCA GTGCTGATGA GAAACCGGCC 
GTCTTATCAC CCCCTTCTGG CGGCGATGTC AAACCAGTCG TGGTCAATAC CATATCAGCA 
GCAACAGCGC CTGTGCTGCC ACCAGCACCA CCACCGCTAT CCTCCTCCCT CGTGGCGGCC 
TGCTCCAACG GAGCCGCCTC TTCGACAACC AGCTCCACAC CCACCTCCAC CTCAGCAAGC 
AACAGCAGCA GCCTCAGCAG CAGCAAACCG AGCAATCCAC CGATGAAAGC ACTGAAATGC 
TCACCGCCCA CAACTGAGGC GCGAGCCAGC ACATCCGCAC ATCCCACCAC GAGCAGCGTG 
TCCACTTTGT ACCAGCCAGC GCAGGTCACT CCGGATTATC GCTTCAACCA GCAGCAGCAG 
CAGCCGCAGA CGTCGGGCTT TGGGCGTGGT GTCACCACCT CAACAGCCGC GCTGGTGATT 
CCGCAGGTGC CTCAGCAGCA CTTCCTCGCC AGCCATCAAC AGCAGGCTCA CTTTCCACAC 
CAGCAGCAGT CCCCGCAGCA ACAGCAGCAA CATTCGCAGC ATCAGCCTGC TCTGAAGTTC 
AGCTACGCCA CAGCTTTGCC CCCACCTCCG CCGTTGCAGC TATTTACGCA GCAACAGCAG 
CAGCAACAGC AGACGGTTGT TCCACCGCAG ACCCAGCAGC AATCGCACCA GAAACCACCT 
CCCGCCTATG GAGCCGCCAT TAGTCAAATA CGATCTCAGC ACAACCAAAA CAAACAGCAG 
CCACAGTCCC AAAGCGTGGC CATGCAACCC AACCTAGCCC TGGCACCAAC GACGACATCA 
GCGGCTTCAA CTTCACACGC AACTGCAGCT GTTAAGCAGC CCAGTAAACC TCATACGGAA 
GTATTCCTGA ATCCCCCACC ACCGCCATCG GCACAGCAAC AGTTGCAGAT CCACAGTTTA 
GTGCAAAATC AAGGTCAACA CCATCAGCAT TCTCACCCGC AGCAGTCGCA GCCGAGCCAG 
CAGAGTCACC AACAGCCACC GAGCTTGCAG ACCTACGGAT TAGGGGTCAG CAATAGCTGC 
AGTTCCTCCA ACAACCCCTC GACCTCGGGA TCTGGTCATC GTGCGAATCG TCAGACAAAC 
TCACCGTTTG GAGCACTGCT TAATGCTGCA GCGGCAGTGG CCTGCGCATC GCCGGCCTGT 
CCCACCACCT CATCTTCGGT ATTGAGGTTT GCAGAATCAC CGGTGGCACA CTACCTTGAG 
CGGGAAAATG GTGACTTCAT TGTCCAGGAG ACACCGAAGC ATATTGTAGA GTGTGTGGAG 
AAGGAAGACG GCGAGTTTTC CGTGATCGAA CGCATTTATC AATCCCCGCC GAGTGTTCTG 
CACATACACG ATGATGATGA GGATGACGAG GATGAAGAGG AGGAGGACGA GCATGATGCT 
CCGCCCGAGG AAGAAGACGG GGACGATGAG CGGGAGGAGA ACAGCCACAG CCGTAATATG 
AGCAAGACAA CGAAAAAGGC CCGTAAGACG AAGCCGAAGC GAGGCTCCAA GCGCATGTCG 
GACGAGGAGG AGTTCATGAG CCTTAGCAGC GGATGTGAGA GTGATTTGGA AAAGGAGGGT 
AAACAGGAGC AGCTAAATGC AGCGGCATCA CTACCACCTC CATTGTGCAC GGCTCCTTCT 
ACAAGTGCTG CGGCTGGAGC TTCCACCTCC AATCTGGCGG ACAATCATTC CAATTCCGCG 
TCCAACTCTG CCTCCAATTC GAACTCCACC GCCACTACAA AGTCCAAGAA GAATCGAATT 
ACCGTACTCA GCGATGTTCC GCTCAATATG AATGAGTACC TCGATTTGAT GGGCAACATC 
GTAGCCTCCA GTCGCATTGG GGCAAGTCGA CCGTTTGCCG CTGTGGCGCC CATACCACTC 
GTCAAGGTGG AGAAGGAAGA ACCTCTGGAT GATTACGACA ACGTGGCAAT GGCCGCTAAT 
GAAGAGCTGG AGCAAAAGCC CAGCCTTGAG CATGGCACCA CTAGCGTCAT TCGAATGGCA 
AGCACGGCTG CAGCATCGAC TTCTGCAACG GCGACCGAAA CATCTGCCGA GGATCTCACT 
CAGCCGCCGC CCATGAAAGT CGAGGTTGAG GCGACCACCA CATTGTTGCC GCAACGCTTT 
TCAACTCCCG CCCAGCATCG CGGGCCTAAG AAATTAGTCA TCAAACCAAA ACAAACAGCA 
CCAGCGACAG CGACGACAAC AAAAAAGACT GACACCCCAT CTTCCCCCAG CAACAACCCA 
TCCATATCAA GAGATCTGGT GCAAGTAAAG AGTGAGACAC TGGAGGCGCC AGCGACGAGC 
AGTAGCAGTA GCATTCTGGA GAAGCACCTA ACCACCAGCC GAAAACTTTT GCATCATCAC 
TCGGCGGTGA ACGATGACGA TGCCCGGGTG CTGCTGGAGT TTGCGAACTC GAAGCAGCCG 
CCGCCACTGG CCGCGTCGAG CACAACGTTT GTGGTTAATG CCAGTGTAGG CTTTCCCTCG 
GCTGGCTCAG TTTTCGCCAG CAGCAGTACC AGCTGTTCCA AAGCACCGGC CAAGCCGGGA 
GAGGAGTACA TCCTGCCGGA CAACAACATG GACGTGGATG AGATTGTGAT ATCCTCGTCG 
TCTTCCTACA CCTCCTCTGC AGCCCAGGTG CAGAACACGT TACAGCCGCC GGACTTTAAC 
TTTCTGTACC ACCAGGCCAC TACAACGGCC ACGACGGCCA CATACTTCAA TCCCTTTTCC 
AGGCAGCAGC AGCATCATCA TCAGCAGCAG CACGGCGTCA GCGATGCCAC CAATGCGGCT 
ACTTTGGCTT CGTCGTCCAG TAATTCGTTG GACCACGAGT CGATGAATGC TACATTCCGG 
GTGGCCAAGA CCCAATCGCT GCAGTCATGG TATCAGCAAG AGCCGGATCA CGATCCACAG 
AATCAGGGTG GTGCGGAATC GGGAACTGTC ACTCCCTCCA ATCAGAGCCA GGATGTGGTT 
AGCCACCAGC CAACAAACTT TAACGCCGCC CTAGCGGCCG TCGATTGCTG TAGCGTTGCC 
TTGGACGAGG ACGCAAAATA TCTTGATCTA GACGCCTGCA AGCGTGAGCA GCTGAGCAGC 
AGTAGCAATC TGCCCTCCGC TTCCGCGTCC ACAACGTCGT CGTCGTTCGC TGGCGCTGGC 
ACGGAGAGCA GTTTGGTGGG TGGTCTGAAT ATCCGAACCG ATGAAAAGAT GCCCGCCAAG 
GGTGAAATTT CCGAACAGGA AAGCAACTGC GACATCGAGA ACTCCTGGAG TGAGTCGGTT 
TATGGGGATA TTTCGCAGCG GTACTTCAGA AACCAGTTTT CTGGCGGCTA CCACCCTGAT 
TGGCGACAGG ACTATTATCC GGCTCAAGAT CTCAGCGCTC AGCTGAGAGA GCATAAACGT 
TTTGACTTTA ATGCTGTCGA TGATGAGGCT TCATCCAGCT CGAGAAGGAG CCATCTTACA 
GATCTCCCGA CAGCGGCCAC CTCCTCCGCA TGTACGTCAT CAGCTATGCT GGCGGGTCCA 
CCAATAGCTT CGACATCACG GGCTGCTGCT GAGGCGGCAG CGGAGGCGGC TGCTCTTGCC 
CAGCGGAAAC CGCAGCGCCG CAAGCTCTAC AAGTGTCCTC ACTGCGAGGC GATCTTCGAA 
AAGCTTAAGG AGCGTAATGC GCACATGATT GGCGAGCACA ACTACGTACG GCAAAATCGC 
CGTCTAATTT GCACTCAGCC GCAAATCAAT ACTTCGATGT TAACGCTACA GCAACAGATG 
ATAGTGCAGT CAGGTGAGGT TGAGGATTCC AAGGATGGCA TTGTGAAGAT AAAACAGGAG 
CAGTGCCAGG AAAAGCAGGA TGTTGAGCAA ATGCACGACC ATTCCGAACT ATTGGCTGAT 
GTCAAGGACT CTGAGCTGAT GGGCATCGGC GGTGATGGTG ACGGTGATGT AGATGACGAC 
ATTAAGCCAC CCAACCAGCT AGATGAAAAG CCCACGCTGC CGCCATTAGC CATGACGACC 
CCAGCGGCTA AATTAGCAGC TTTGTATCGA ATGCTCATCG CCTACAACGA ATCCAAGCTC 
GGACAGGAGC GCAACAATCT TAGCGAGCTG GAGCAGAAGG CTATGGAAAA ATCCATATTT 
CTCTGCTATG TCTGTCGTAC CGACTTCCCG TCCGTAAAGC TCTATGACGC CCATCTCACC 
GAGCATCCCG CTGAATGCTT TACGTGCGGC AAGAAGTTCT ACCGTTGGAA GAACTTCTCA 
TTGCACTTGA AGCGGCACTT GGGCTGGAAG GAGTTCGGAT GCTATGTCTG CGACAAGAAG 
TTCGTAGTGC GCAGCGCCTT GGTCGAACAC ATGCGGATGC ACACGGGGCA AACGCCACTC 
AAGTGCAAGA TATGCGGTAA AAAGTTCAAG AGATACTCGA ATCTGACGCA ACACCGCAAA 
CGCCACACCA AAATGATGGT GCGCAAGAAG GAATACGTGT GTCACTGCGG CGAAGTCTTG 
CCCTCGAAGG CTCGCTTCCT GTGGCACAAG GAAACGCACG ACCTGAAGCC GAAGTGCTGC 
CCGTACTGCT GCGATCGGTT TGTGCACGCC AACTCGCTAC GTCGACACAT ACGGTTGGCC 
CACTCTGATA AGTTTGACTA TGCCGAACCT ATGGAATGCC CTATGTGTAA GCAAATCTTC 
GCGAAGACGT CCATCAAGGC GCACATGGCG ACGCACTCAA CGGAGCCCCA ATACGATTGC 
GCCATTTGTA GCAAGAGCTT CTCCACCAAA TGGAATCTCA AGATCCACTC GTGGGTACAC 
GCCAACCGGA CGGCCAAGCC CTTTAAGTGC GAATACTGCC CAAAGGCGTT TGTGCGAGAG 
CTGGACTTCA AGAACCACAT CAACGCGCAC AAACAGATCA AACCGTACAC GTGCGAGTAC 
TGCGGCTGCA AGTTCATCCG CAAGTACAAT TACATGCGAC ACCGGCGCGA ACATCATGGC 
ACCAAGAAGT TCACCTGCGA CCAATGCGAT AAGTCTTTCC ATCGGCATTA CTATCTGATA 
GAGCACCGGC GAATGCATAC CGGGGAGCGT CCGTTCACGT GTACCATCTG CGGCAAGAGC 
TCCACAACGA AGACCAACCA CAACAAGCAT CTGAAGATCC ACCATTCGCG CGATCCGTTC 
ACGGTGGAGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287396.1
CDS	259..5613
Translation	MVDNSVQCPVCTLYLHAGMNLSDHLETHPKEQVIKALVQMTIVGNGGNSMGSVLAAAMLTGGSGGGGSSGGEASADEKPAVLSPPSGGDVKPVVVNTISAATAPVLPPAPPPLSSSLVAACSNGAASSTTSSTPTSTSASNSSSLSSSKPSNPPMKALKCSPPTTEARASTSAHPTTSSVSTLYQPAQVTPDYRFNQQQQQPQTSGFGRGVTTSTAALVIPQVPQQHFLASHQQQAHFPHQQQSPQQQQQHSQHQPALKFSYATALPPPPPLQLFTQQQQQQQQTVVPPQTQQQSHQKPPPAYGAAISQIRSQHNQNKQQPQSQSVAMQPNLALAPTTTSAASTSHATAAVKQPSKPHTEVFLNPPPPPSAQQQLQIHSLVQNQGQHHQHSHPQQSQPSQQSHQQPPSLQTYGLGVSNSCSSSNNPSTSGSGHRANRQTNSPFGALLNAAAAVACASPACPTTSSSVLRFAESPVAHYLERENGDFIVQETPKHIVECVEKEDGEFSVIERIYQSPPSVLHIHDDDEDDEDEEEEDEHDAPPEEEDGDDEREENSHSRNMSKTTKKARKTKPKRGSKRMSDEEEFMSLSSGCESDLEKEGKQEQLNAAASLPPPLCTAPSTSAAAGASTSNLADNHSNSASNSASNSNSTATTKSKKNRITVLSDVPLNMNEYLDLMGNIVASSRIGASRPFAAVAPIPLVKVEKEEPLDDYDNVAMAANEELEQKPSLEHGTTSVIRMASTAAASTSATATETSAEDLTQPPPMKVEVEATTTLLPQRFSTPAQHRGPKKLVIKPKQTAPATATTTKKTDTPSSPSNNPSISRDLVQVKSETLEAPATSSSSSILEKHLTTSRKLLHHHSAVNDDDARVLLEFANSKQPPPLAASSTTFVVNASVGFPSAGSVFASSSTSCSKAPAKPGEEYILPDNNMDVDEIVISSSSSYTSSAAQVQNTLQPPDFNFLYHQATTTATTATYFNPFSRQQQHHHQQQHGVSDATNAATLASSSSNSLDHESMNATFRVAKTQSLQSWYQQEPDHDPQNQGGAESGTVTPSNQSQDVVSHQPTNFNAALAAVDCCSVALDEDAKYLDLDACKREQLSSSSNLPSASASTTSSSFAGAGTESSLVGGLNIRTDEKMPAKGEISEQESNCDIENSWSESVYGDISQRYFRNQFSGGYHPDWRQDYYPAQDLSAQLREHKRFDFNAVDDEASSSSRRSHLTDLPTAATSSACTSSAMLAGPPIASTSRAAAEAAAEAAALAQRKPQRRKLYKCPHCEAIFEKLKERNAHMIGEHNYVRQNRRLICTQPQINTSMLTLQQQMIVQSGEVEDSKDGIVKIKQEQCQEKQDVEQMHDHSELLADVKDSELMGIGGDGDGDVDDDIKPPNQLDEKPTLPPLAMTTPAAKLAALYRMLIAYNESKLGQERNNLSELEQKAMEKSIFLCYVCRTDFPSVKLYDAHLTEHPAECFTCGKKFYRWKNFSLHLKRHLGWKEFGCYVCDKKFVVRSALVEHMRMHTGQTPLKCKICGKKFKRYSNLTQHRKRHTKMMVRKKEYVCHCGEVLPSKARFLWHKETHDLKPKCCPYCCDRFVHANSLRRHIRLAHSDKFDYAEPMECPMCKQIFAKTSIKAHMATHSTEPQYDCAICSKSFSTKWNLKIHSWVHANRTAKPFKCEYCPKAFVRELDFKNHINAHKQIKPYTCEYCGCKFIRKYNYMRHRREHHGTKKFTCDQCDKSFHRHYYLIEHRRMHTGERPFTCTICGKSSTTKTNHNKHLKIHHSRDPFTVEA

Target ORF information:

RefSeq Version	NM_001300467.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG31224), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300467.1

ORF Insert Sequence:

ATGGTCGACA ACAGTGTCCA ATGCCCCGTG TGCACATTGT ACCTGCATGC GGGCATGAAT 
CTCTCCGATC ACTTGGAGAC CCATCCCAAG GAGCAGGTGA TCAAGGCGCT GGTGCAGATG 
ACGATCGTTG GGAACGGTGG CAACAGCATG GGCAGCGTTC TCGCTGCCGC CATGTTGACA 
GGAGGATCTG GAGGAGGTGG ATCCAGCGGC GGCGAAGCCA GTGCTGATGA GAAACCGGCC 
GTCTTATCAC CCCCTTCTGG CGGCGATGTC AAACCAGTCG TGGTCAATAC CATATCAGCA 
GCAACAGCGC CTGTGCTGCC ACCAGCACCA CCACCGCTAT CCTCCTCCCT CGTGGCGGCC 
TGCTCCAACG GAGCCGCCTC TTCGACAACC AGCTCCACAC CCACCTCCAC CTCAGCAAGC 
AACAGCAGCA GCCTCAGCAG CAGCAAACCG AGCAATCCAC CGATGAAAGC ACTGAAATGC 
TCACCGCCCA CAACTGAGGC GCGAGCCAGC ACATCCGCAC ATCCCACCAC GAGCAGCGTG 
TCCACTTTGT ACCAGCCAGC GCAGGTCACT CCGGATTATC GCTTCAACCA GCAGCAGCAG 
CAGCCGCAGA CGTCGGGCTT TGGGCGTGGT GTCACCACCT CAACAGCCGC GCTGGTGATT 
CCGCAGGTGC CTCAGCAGCA CTTCCTCGCC AGCCATCAAC AGCAGGCTCA CTTTCCACAC 
CAGCAGCAGT CCCCGCAGCA ACAGCAGCAA CATTCGCAGC ATCAGCCTGC TCTGAAGTTC 
AGCTACGCCA CAGCTTTGCC CCCACCTCCG CCGTTGCAGC TATTTACGCA GCAACAGCAG 
CAGCAACAGC AGACGGTTGT TCCACCGCAG ACCCAGCAGC AATCGCACCA GAAACCACCT 
CCCGCCTATG GAGCCGCCAT TAGTCAAATA CGATCTCAGC ACAACCAAAA CAAACAGCAG 
CCACAGTCCC AAAGCGTGGC CATGCAACCC AACCTAGCCC TGGCACCAAC GACGACATCA 
GCGGCTTCAA CTTCACACGC AACTGCAGCT GTTAAGCAGC CCAGTAAACC TCATACGGAA 
GTATTCCTGA ATCCCCCACC ACCGCCATCG GCACAGCAAC AGTTGCAGAT CCACAGTTTA 
GTGCAAAATC AAGGTCAACA CCATCAGCAT TCTCACCCGC AGCAGTCGCA GCCGAGCCAG 
CAGAGTCACC AACAGCCACC GAGCTTGCAG ACCTACGGAT TAGGGGTCAG CAATAGCTGC 
AGTTCCTCCA ACAACCCCTC GACCTCGGGA TCTGGTCATC GTGCGAATCG TCAGACAAAC 
TCACCGTTTG GAGCACTGCT TAATGCTGCA GCGGCAGTGG CCTGCGCATC GCCGGCCTGT 
CCCACCACCT CATCTTCGGT ATTGAGGTTT GCAGAATCAC CGGTGGCACA CTACCTTGAG 
CGGGAAAATG GTGACTTCAT TGTCCAGGAG ACACCGAAGC ATATTGTAGA GTGTGTGGAG 
AAGGAAGACG GCGAGTTTTC CGTGATCGAA CGCATTTATC AATCCCCGCC GAGTGTTCTG 
CACATACACG ATGATGATGA GGATGACGAG GATGAAGAGG AGGAGGACGA GCATGATGCT 
CCGCCCGAGG AAGAAGACGG GGACGATGAG CGGGAGGAGA ACAGCCACAG CCGTAATATG 
AGCAAGACAA CGAAAAAGGC CCGTAAGACG AAGCCGAAGC GAGGCTCCAA GCGCATGTCG 
GACGAGGAGG AGTTCATGAG CCTTAGCAGC GGATGTGAGA GTGATTTGGA AAAGGAGGGT 
AAACAGGAGC AGCTAAATGC AGCGGCATCA CTACCACCTC CATTGTGCAC GGCTCCTTCT 
ACAAGTGCTG CGGCTGGAGC TTCCACCTCC AATCTGGCGG ACAATCATTC CAATTCCGCG 
TCCAACTCTG CCTCCAATTC GAACTCCACC GCCACTACAA AGTCCAAGAA GAATCGAATT 
ACCGTACTCA GCGATGTTCC GCTCAATATG AATGAGTACC TCGATTTGAT GGGCAACATC 
GTAGCCTCCA GTCGCATTGG GGCAAGTCGA CCGTTTGCCG CTGTGGCGCC CATACCACTC 
GTCAAGGTGG AGAAGGAAGA ACCTCTGGAT GATTACGACA ACGTGGCAAT GGCCGCTAAT 
GAAGAGCTGG AGCAAAAGCC CAGCCTTGAG CATGGCACCA CTAGCGTCAT TCGAATGGCA 
AGCACGGCTG CAGCATCGAC TTCTGCAACG GCGACCGAAA CATCTGCCGA GGATCTCACT 
CAGCCGCCGC CCATGAAAGT CGAGGTTGAG GCGACCACCA CATTGTTGCC GCAACGCTTT 
TCAACTCCCG CCCAGCATCG CGGGCCTAAG AAATTAGTCA TCAAACCAAA ACAAACAGCA 
CCAGCGACAG CGACGACAAC AAAAAAGACT GACACCCCAT CTTCCCCCAG CAACAACCCA 
TCCATATCAA GAGATCTGGT GCAAGTAAAG AGTGAGACAC TGGAGGCGCC AGCGACGAGC 
AGTAGCAGTA GCATTCTGGA GAAGCACCTA ACCACCAGCC GAAAACTTTT GCATCATCAC 
TCGGCGGTGA ACGATGACGA TGCCCGGGTG CTGCTGGAGT TTGCGAACTC GAAGCAGCCG 
CCGCCACTGG CCGCGTCGAG CACAACGTTT GTGGTTAATG CCAGTGTAGG CTTTCCCTCG 
GCTGGCTCAG TTTTCGCCAG CAGCAGTACC AGCTGTTCCA AAGCACCGGC CAAGCCGGGA 
GAGGAGTACA TCCTGCCGGA CAACAACATG GACGTGGATG AGATTGTGAT ATCCTCGTCG 
TCTTCCTACA CCTCCTCTGC AGCCCAGGTG CAGAACACGT TACAGCCGCC GGACTTTAAC 
TTTCTGTACC ACCAGGCCAC TACAACGGCC ACGACGGCCA CATACTTCAA TCCCTTTTCC 
AGGCAGCAGC AGCATCATCA TCAGCAGCAG CACGGCGTCA GCGATGCCAC CAATGCGGCT 
ACTTTGGCTT CGTCGTCCAG TAATTCGTTG GACCACGAGT CGATGAATGC TACATTCCGG 
GTGGCCAAGA CCCAATCGCT GCAGTCATGG TATCAGCAAG AGCCGGATCA CGATCCACAG 
AATCAGGGTG GTGCGGAATC GGGAACTGTC ACTCCCTCCA ATCAGAGCCA GGATGTGGTT 
AGCCACCAGC CAACAAACTT TAACGCCGCC CTAGCGGCCG TCGATTGCTG TAGCGTTGCC 
TTGGACGAGG ACGCAAAATA TCTTGATCTA GACGCCTGCA AGCGTGAGCA GCTGAGCAGC 
AGTAGCAATC TGCCCTCCGC TTCCGCGTCC ACAACGTCGT CGTCGTTCGC TGGCGCTGGC 
ACGGAGAGCA GTTTGGTGGG TGGTCTGAAT ATCCGAACCG ATGAAAAGAT GCCCGCCAAG 
GGTGAAATTT CCGAACAGGA AAGCAACTGC GACATCGAGA ACTCCTGGAG TGAGTCGGTT 
TATGGGGATA TTTCGCAGCG GTACTTCAGA AACCAGTTTT CTGGCGGCTA CCACCCTGAT 
TGGCGACAGG ACTATTATCC GGCTCAAGAT CTCAGCGCTC AGCTGAGAGA GCATAAACGT 
TTTGACTTTA ATGCTGTCGA TGATGAGGCT TCATCCAGCT CGAGAAGGAG CCATCTTACA 
GATCTCCCGA CAGCGGCCAC CTCCTCCGCA TGTACGTCAT CAGCTATGCT GGCGGGTCCA 
CCAATAGCTT CGACATCACG GGCTGCTGCT GAGGCGGCAG CGGAGGCGGC TGCTCTTGCC 
CAGCGGAAAC CGCAGCGCCG CAAGCTCTAC AAGTGTCCTC ACTGCGAGGC GATCTTCGAA 
AAGCTTAAGG AGCGTAATGC GCACATGATT GGCGAGCACA ACTACGTACG GCAAAATCGC 
CGTCTAATTT GCACTCAGCC GCAAATCAAT ACTTCGATGT TAACGCTACA GCAACAGATG 
ATAGTGCAGT CAGGTGAGGT TGAGGATTCC AAGGATGGCA TTGTGAAGAT AAAACAGGAG 
CAGTGCCAGG AAAAGCAGGA TGTTGAGCAA ATGCACGACC ATTCCGAACT ATTGGCTGAT 
GTCAAGGACT CTGAGCTGAT GGGCATCGGC GGTGATGGTG ACGGTGATGT AGATGACGAC 
ATTAAGCCAC CCAACCAGCT AGATGAAAAG CCCACGCTGC CGCCATTAGC CATGACGACC 
CCAGCGGCTA AATTAGCAGC TTTGTATCGA ATGCTCATCG CCTACAACGA ATCCAAGCTC 
GGACAGGAGC GCAACAATCT TAGCGAGCTG GAGCAGAAGG CTATGGAAAA ATCCATATTT 
CTCTGCTATG TCTGTCGTAC CGACTTCCCG TCCGTAAAGC TCTATGACGC CCATCTCACC 
GAGCATCCCG CTGAATGCTT TACGTGCGGC AAGAAGTTCT ACCGTTGGAA GAACTTCTCA 
TTGCACTTGA AGCGGCACTT GGGCTGGAAG GAGTTCGGAT GCTATGTCTG CGACAAGAAG 
TTCGTAGTGC GCAGCGCCTT GGTCGAACAC ATGCGGATGC ACACGGGGCA AACGCCACTC 
AAGTGCAAGA TATGCGGTAA AAAGTTCAAG AGATACTCGA ATCTGACGCA ACACCGCAAA 
CGCCACACCA AAATGATGGT GCGCAAGAAG GAATACGTGT GTCACTGCGG CGAAGTCTTG 
CCCTCGAAGG CTCGCTTCCT GTGGCACAAG GAAACGCACG ACCTGAAGCC GAAGTGCTGC 
CCGTACTGCT GCGATCGGTT TGTGCACGCC AACTCGCTAC GTCGACACAT ACGGTTGGCC 
CACTCTGATA AGTTTGACTA TGCCGAACCT ATGGAATGCC CTATGTGTAA GCAAATCTTC 
GCGAAGACGT CCATCAAGGC GCACATGGCG ACGCACTCAA CGGAGCCCCA ATACGATTGC 
GCCATTTGTA GCAAGAGCTT CTCCACCAAA TGGAATCTCA AGATCCACTC GTGGGTACAC 
GCCAACCGGA CGGCCAAGCC CTTTAAGTGC GAATACTGCC CAAAGGCGTT TGTGCGAGAG 
CTGGACTTCA AGAACCACAT CAACGCGCAC AAACAGATCA AACCGTACAC GTGCGAGTAC 
TGCGGCTGCA AGTTCATCCG CAAGTACAAT TACATGCGAC ACCGGCGCGA ACATCATGGC 
ACCAAGAAGT TCACCTGCGA CCAATGCGAT AAGTCTTTCC ATCGGCATTA CTATCTGATA 
GAGCACCGGC GAATGCATAC CGGGGAGCGT CCGTTCACGT GTACCATCTG CGGCAAGAGC 
TCCACAACGA AGACCAACCA CAACAAGCAT CTGAAGATCC ACCATTCGCG CGATCCGTTC 
ACGGTGGAGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG31224 ( NM_001300468.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287397.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG31224), mRNA.

Price & Availability↑

CloneID	OFa05326
Clone ID Related Accession (Same CDS sequence)	NM_169826.2 , NM_001300467.1 , NM_001300468.1
Accession Version	NM_001300468.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTCGACA ACAGTGTCCA ATGCCCCGTG TGCACATTGT ACCTGCATGC GGGCATGAAT 
CTCTCCGATC ACTTGGAGAC CCATCCCAAG GAGCAGGTGA TCAAGGCGCT GGTGCAGATG 
ACGATCGTTG GGAACGGTGG CAACAGCATG GGCAGCGTTC TCGCTGCCGC CATGTTGACA 
GGAGGATCTG GAGGAGGTGG ATCCAGCGGC GGCGAAGCCA GTGCTGATGA GAAACCGGCC 
GTCTTATCAC CCCCTTCTGG CGGCGATGTC AAACCAGTCG TGGTCAATAC CATATCAGCA 
GCAACAGCGC CTGTGCTGCC ACCAGCACCA CCACCGCTAT CCTCCTCCCT CGTGGCGGCC 
TGCTCCAACG GAGCCGCCTC TTCGACAACC AGCTCCACAC CCACCTCCAC CTCAGCAAGC 
AACAGCAGCA GCCTCAGCAG CAGCAAACCG AGCAATCCAC CGATGAAAGC ACTGAAATGC 
TCACCGCCCA CAACTGAGGC GCGAGCCAGC ACATCCGCAC ATCCCACCAC GAGCAGCGTG 
TCCACTTTGT ACCAGCCAGC GCAGGTCACT CCGGATTATC GCTTCAACCA GCAGCAGCAG 
CAGCCGCAGA CGTCGGGCTT TGGGCGTGGT GTCACCACCT CAACAGCCGC GCTGGTGATT 
CCGCAGGTGC CTCAGCAGCA CTTCCTCGCC AGCCATCAAC AGCAGGCTCA CTTTCCACAC 
CAGCAGCAGT CCCCGCAGCA ACAGCAGCAA CATTCGCAGC ATCAGCCTGC TCTGAAGTTC 
AGCTACGCCA CAGCTTTGCC CCCACCTCCG CCGTTGCAGC TATTTACGCA GCAACAGCAG 
CAGCAACAGC AGACGGTTGT TCCACCGCAG ACCCAGCAGC AATCGCACCA GAAACCACCT 
CCCGCCTATG GAGCCGCCAT TAGTCAAATA CGATCTCAGC ACAACCAAAA CAAACAGCAG 
CCACAGTCCC AAAGCGTGGC CATGCAACCC AACCTAGCCC TGGCACCAAC GACGACATCA 
GCGGCTTCAA CTTCACACGC AACTGCAGCT GTTAAGCAGC CCAGTAAACC TCATACGGAA 
GTATTCCTGA ATCCCCCACC ACCGCCATCG GCACAGCAAC AGTTGCAGAT CCACAGTTTA 
GTGCAAAATC AAGGTCAACA CCATCAGCAT TCTCACCCGC AGCAGTCGCA GCCGAGCCAG 
CAGAGTCACC AACAGCCACC GAGCTTGCAG ACCTACGGAT TAGGGGTCAG CAATAGCTGC 
AGTTCCTCCA ACAACCCCTC GACCTCGGGA TCTGGTCATC GTGCGAATCG TCAGACAAAC 
TCACCGTTTG GAGCACTGCT TAATGCTGCA GCGGCAGTGG CCTGCGCATC GCCGGCCTGT 
CCCACCACCT CATCTTCGGT ATTGAGGTTT GCAGAATCAC CGGTGGCACA CTACCTTGAG 
CGGGAAAATG GTGACTTCAT TGTCCAGGAG ACACCGAAGC ATATTGTAGA GTGTGTGGAG 
AAGGAAGACG GCGAGTTTTC CGTGATCGAA CGCATTTATC AATCCCCGCC GAGTGTTCTG 
CACATACACG ATGATGATGA GGATGACGAG GATGAAGAGG AGGAGGACGA GCATGATGCT 
CCGCCCGAGG AAGAAGACGG GGACGATGAG CGGGAGGAGA ACAGCCACAG CCGTAATATG 
AGCAAGACAA CGAAAAAGGC CCGTAAGACG AAGCCGAAGC GAGGCTCCAA GCGCATGTCG 
GACGAGGAGG AGTTCATGAG CCTTAGCAGC GGATGTGAGA GTGATTTGGA AAAGGAGGGT 
AAACAGGAGC AGCTAAATGC AGCGGCATCA CTACCACCTC CATTGTGCAC GGCTCCTTCT 
ACAAGTGCTG CGGCTGGAGC TTCCACCTCC AATCTGGCGG ACAATCATTC CAATTCCGCG 
TCCAACTCTG CCTCCAATTC GAACTCCACC GCCACTACAA AGTCCAAGAA GAATCGAATT 
ACCGTACTCA GCGATGTTCC GCTCAATATG AATGAGTACC TCGATTTGAT GGGCAACATC 
GTAGCCTCCA GTCGCATTGG GGCAAGTCGA CCGTTTGCCG CTGTGGCGCC CATACCACTC 
GTCAAGGTGG AGAAGGAAGA ACCTCTGGAT GATTACGACA ACGTGGCAAT GGCCGCTAAT 
GAAGAGCTGG AGCAAAAGCC CAGCCTTGAG CATGGCACCA CTAGCGTCAT TCGAATGGCA 
AGCACGGCTG CAGCATCGAC TTCTGCAACG GCGACCGAAA CATCTGCCGA GGATCTCACT 
CAGCCGCCGC CCATGAAAGT CGAGGTTGAG GCGACCACCA CATTGTTGCC GCAACGCTTT 
TCAACTCCCG CCCAGCATCG CGGGCCTAAG AAATTAGTCA TCAAACCAAA ACAAACAGCA 
CCAGCGACAG CGACGACAAC AAAAAAGACT GACACCCCAT CTTCCCCCAG CAACAACCCA 
TCCATATCAA GAGATCTGGT GCAAGTAAAG AGTGAGACAC TGGAGGCGCC AGCGACGAGC 
AGTAGCAGTA GCATTCTGGA GAAGCACCTA ACCACCAGCC GAAAACTTTT GCATCATCAC 
TCGGCGGTGA ACGATGACGA TGCCCGGGTG CTGCTGGAGT TTGCGAACTC GAAGCAGCCG 
CCGCCACTGG CCGCGTCGAG CACAACGTTT GTGGTTAATG CCAGTGTAGG CTTTCCCTCG 
GCTGGCTCAG TTTTCGCCAG CAGCAGTACC AGCTGTTCCA AAGCACCGGC CAAGCCGGGA 
GAGGAGTACA TCCTGCCGGA CAACAACATG GACGTGGATG AGATTGTGAT ATCCTCGTCG 
TCTTCCTACA CCTCCTCTGC AGCCCAGGTG CAGAACACGT TACAGCCGCC GGACTTTAAC 
TTTCTGTACC ACCAGGCCAC TACAACGGCC ACGACGGCCA CATACTTCAA TCCCTTTTCC 
AGGCAGCAGC AGCATCATCA TCAGCAGCAG CACGGCGTCA GCGATGCCAC CAATGCGGCT 
ACTTTGGCTT CGTCGTCCAG TAATTCGTTG GACCACGAGT CGATGAATGC TACATTCCGG 
GTGGCCAAGA CCCAATCGCT GCAGTCATGG TATCAGCAAG AGCCGGATCA CGATCCACAG 
AATCAGGGTG GTGCGGAATC GGGAACTGTC ACTCCCTCCA ATCAGAGCCA GGATGTGGTT 
AGCCACCAGC CAACAAACTT TAACGCCGCC CTAGCGGCCG TCGATTGCTG TAGCGTTGCC 
TTGGACGAGG ACGCAAAATA TCTTGATCTA GACGCCTGCA AGCGTGAGCA GCTGAGCAGC 
AGTAGCAATC TGCCCTCCGC TTCCGCGTCC ACAACGTCGT CGTCGTTCGC TGGCGCTGGC 
ACGGAGAGCA GTTTGGTGGG TGGTCTGAAT ATCCGAACCG ATGAAAAGAT GCCCGCCAAG 
GGTGAAATTT CCGAACAGGA AAGCAACTGC GACATCGAGA ACTCCTGGAG TGAGTCGGTT 
TATGGGGATA TTTCGCAGCG GTACTTCAGA AACCAGTTTT CTGGCGGCTA CCACCCTGAT 
TGGCGACAGG ACTATTATCC GGCTCAAGAT CTCAGCGCTC AGCTGAGAGA GCATAAACGT 
TTTGACTTTA ATGCTGTCGA TGATGAGGCT TCATCCAGCT CGAGAAGGAG CCATCTTACA 
GATCTCCCGA CAGCGGCCAC CTCCTCCGCA TGTACGTCAT CAGCTATGCT GGCGGGTCCA 
CCAATAGCTT CGACATCACG GGCTGCTGCT GAGGCGGCAG CGGAGGCGGC TGCTCTTGCC 
CAGCGGAAAC CGCAGCGCCG CAAGCTCTAC AAGTGTCCTC ACTGCGAGGC GATCTTCGAA 
AAGCTTAAGG AGCGTAATGC GCACATGATT GGCGAGCACA ACTACGTACG GCAAAATCGC 
CGTCTAATTT GCACTCAGCC GCAAATCAAT ACTTCGATGT TAACGCTACA GCAACAGATG 
ATAGTGCAGT CAGGTGAGGT TGAGGATTCC AAGGATGGCA TTGTGAAGAT AAAACAGGAG 
CAGTGCCAGG AAAAGCAGGA TGTTGAGCAA ATGCACGACC ATTCCGAACT ATTGGCTGAT 
GTCAAGGACT CTGAGCTGAT GGGCATCGGC GGTGATGGTG ACGGTGATGT AGATGACGAC 
ATTAAGCCAC CCAACCAGCT AGATGAAAAG CCCACGCTGC CGCCATTAGC CATGACGACC 
CCAGCGGCTA AATTAGCAGC TTTGTATCGA ATGCTCATCG CCTACAACGA ATCCAAGCTC 
GGACAGGAGC GCAACAATCT TAGCGAGCTG GAGCAGAAGG CTATGGAAAA ATCCATATTT 
CTCTGCTATG TCTGTCGTAC CGACTTCCCG TCCGTAAAGC TCTATGACGC CCATCTCACC 
GAGCATCCCG CTGAATGCTT TACGTGCGGC AAGAAGTTCT ACCGTTGGAA GAACTTCTCA 
TTGCACTTGA AGCGGCACTT GGGCTGGAAG GAGTTCGGAT GCTATGTCTG CGACAAGAAG 
TTCGTAGTGC GCAGCGCCTT GGTCGAACAC ATGCGGATGC ACACGGGGCA AACGCCACTC 
AAGTGCAAGA TATGCGGTAA AAAGTTCAAG AGATACTCGA ATCTGACGCA ACACCGCAAA 
CGCCACACCA AAATGATGGT GCGCAAGAAG GAATACGTGT GTCACTGCGG CGAAGTCTTG 
CCCTCGAAGG CTCGCTTCCT GTGGCACAAG GAAACGCACG ACCTGAAGCC GAAGTGCTGC 
CCGTACTGCT GCGATCGGTT TGTGCACGCC AACTCGCTAC GTCGACACAT ACGGTTGGCC 
CACTCTGATA AGTTTGACTA TGCCGAACCT ATGGAATGCC CTATGTGTAA GCAAATCTTC 
GCGAAGACGT CCATCAAGGC GCACATGGCG ACGCACTCAA CGGAGCCCCA ATACGATTGC 
GCCATTTGTA GCAAGAGCTT CTCCACCAAA TGGAATCTCA AGATCCACTC GTGGGTACAC 
GCCAACCGGA CGGCCAAGCC CTTTAAGTGC GAATACTGCC CAAAGGCGTT TGTGCGAGAG 
CTGGACTTCA AGAACCACAT CAACGCGCAC AAACAGATCA AACCGTACAC GTGCGAGTAC 
TGCGGCTGCA AGTTCATCCG CAAGTACAAT TACATGCGAC ACCGGCGCGA ACATCATGGC 
ACCAAGAAGT TCACCTGCGA CCAATGCGAT AAGTCTTTCC ATCGGCATTA CTATCTGATA 
GAGCACCGGC GAATGCATAC CGGGGAGCGT CCGTTCACGT GTACCATCTG CGGCAAGAGC 
TCCACAACGA AGACCAACCA CAACAAGCAT CTGAAGATCC ACCATTCGCG CGATCCGTTC 
ACGGTGGAGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287397.1
CDS	987..6341
Translation	MVDNSVQCPVCTLYLHAGMNLSDHLETHPKEQVIKALVQMTIVGNGGNSMGSVLAAAMLTGGSGGGGSSGGEASADEKPAVLSPPSGGDVKPVVVNTISAATAPVLPPAPPPLSSSLVAACSNGAASSTTSSTPTSTSASNSSSLSSSKPSNPPMKALKCSPPTTEARASTSAHPTTSSVSTLYQPAQVTPDYRFNQQQQQPQTSGFGRGVTTSTAALVIPQVPQQHFLASHQQQAHFPHQQQSPQQQQQHSQHQPALKFSYATALPPPPPLQLFTQQQQQQQQTVVPPQTQQQSHQKPPPAYGAAISQIRSQHNQNKQQPQSQSVAMQPNLALAPTTTSAASTSHATAAVKQPSKPHTEVFLNPPPPPSAQQQLQIHSLVQNQGQHHQHSHPQQSQPSQQSHQQPPSLQTYGLGVSNSCSSSNNPSTSGSGHRANRQTNSPFGALLNAAAAVACASPACPTTSSSVLRFAESPVAHYLERENGDFIVQETPKHIVECVEKEDGEFSVIERIYQSPPSVLHIHDDDEDDEDEEEEDEHDAPPEEEDGDDEREENSHSRNMSKTTKKARKTKPKRGSKRMSDEEEFMSLSSGCESDLEKEGKQEQLNAAASLPPPLCTAPSTSAAAGASTSNLADNHSNSASNSASNSNSTATTKSKKNRITVLSDVPLNMNEYLDLMGNIVASSRIGASRPFAAVAPIPLVKVEKEEPLDDYDNVAMAANEELEQKPSLEHGTTSVIRMASTAAASTSATATETSAEDLTQPPPMKVEVEATTTLLPQRFSTPAQHRGPKKLVIKPKQTAPATATTTKKTDTPSSPSNNPSISRDLVQVKSETLEAPATSSSSSILEKHLTTSRKLLHHHSAVNDDDARVLLEFANSKQPPPLAASSTTFVVNASVGFPSAGSVFASSSTSCSKAPAKPGEEYILPDNNMDVDEIVISSSSSYTSSAAQVQNTLQPPDFNFLYHQATTTATTATYFNPFSRQQQHHHQQQHGVSDATNAATLASSSSNSLDHESMNATFRVAKTQSLQSWYQQEPDHDPQNQGGAESGTVTPSNQSQDVVSHQPTNFNAALAAVDCCSVALDEDAKYLDLDACKREQLSSSSNLPSASASTTSSSFAGAGTESSLVGGLNIRTDEKMPAKGEISEQESNCDIENSWSESVYGDISQRYFRNQFSGGYHPDWRQDYYPAQDLSAQLREHKRFDFNAVDDEASSSSRRSHLTDLPTAATSSACTSSAMLAGPPIASTSRAAAEAAAEAAALAQRKPQRRKLYKCPHCEAIFEKLKERNAHMIGEHNYVRQNRRLICTQPQINTSMLTLQQQMIVQSGEVEDSKDGIVKIKQEQCQEKQDVEQMHDHSELLADVKDSELMGIGGDGDGDVDDDIKPPNQLDEKPTLPPLAMTTPAAKLAALYRMLIAYNESKLGQERNNLSELEQKAMEKSIFLCYVCRTDFPSVKLYDAHLTEHPAECFTCGKKFYRWKNFSLHLKRHLGWKEFGCYVCDKKFVVRSALVEHMRMHTGQTPLKCKICGKKFKRYSNLTQHRKRHTKMMVRKKEYVCHCGEVLPSKARFLWHKETHDLKPKCCPYCCDRFVHANSLRRHIRLAHSDKFDYAEPMECPMCKQIFAKTSIKAHMATHSTEPQYDCAICSKSFSTKWNLKIHSWVHANRTAKPFKCEYCPKAFVRELDFKNHINAHKQIKPYTCEYCGCKFIRKYNYMRHRREHHGTKKFTCDQCDKSFHRHYYLIEHRRMHTGERPFTCTICGKSSTTKTNHNKHLKIHHSRDPFTVEA

Target ORF information:

RefSeq Version	NM_001300468.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG31224), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300468.1

ORF Insert Sequence:

ATGGTCGACA ACAGTGTCCA ATGCCCCGTG TGCACATTGT ACCTGCATGC GGGCATGAAT 
CTCTCCGATC ACTTGGAGAC CCATCCCAAG GAGCAGGTGA TCAAGGCGCT GGTGCAGATG 
ACGATCGTTG GGAACGGTGG CAACAGCATG GGCAGCGTTC TCGCTGCCGC CATGTTGACA 
GGAGGATCTG GAGGAGGTGG ATCCAGCGGC GGCGAAGCCA GTGCTGATGA GAAACCGGCC 
GTCTTATCAC CCCCTTCTGG CGGCGATGTC AAACCAGTCG TGGTCAATAC CATATCAGCA 
GCAACAGCGC CTGTGCTGCC ACCAGCACCA CCACCGCTAT CCTCCTCCCT CGTGGCGGCC 
TGCTCCAACG GAGCCGCCTC TTCGACAACC AGCTCCACAC CCACCTCCAC CTCAGCAAGC 
AACAGCAGCA GCCTCAGCAG CAGCAAACCG AGCAATCCAC CGATGAAAGC ACTGAAATGC 
TCACCGCCCA CAACTGAGGC GCGAGCCAGC ACATCCGCAC ATCCCACCAC GAGCAGCGTG 
TCCACTTTGT ACCAGCCAGC GCAGGTCACT CCGGATTATC GCTTCAACCA GCAGCAGCAG 
CAGCCGCAGA CGTCGGGCTT TGGGCGTGGT GTCACCACCT CAACAGCCGC GCTGGTGATT 
CCGCAGGTGC CTCAGCAGCA CTTCCTCGCC AGCCATCAAC AGCAGGCTCA CTTTCCACAC 
CAGCAGCAGT CCCCGCAGCA ACAGCAGCAA CATTCGCAGC ATCAGCCTGC TCTGAAGTTC 
AGCTACGCCA CAGCTTTGCC CCCACCTCCG CCGTTGCAGC TATTTACGCA GCAACAGCAG 
CAGCAACAGC AGACGGTTGT TCCACCGCAG ACCCAGCAGC AATCGCACCA GAAACCACCT 
CCCGCCTATG GAGCCGCCAT TAGTCAAATA CGATCTCAGC ACAACCAAAA CAAACAGCAG 
CCACAGTCCC AAAGCGTGGC CATGCAACCC AACCTAGCCC TGGCACCAAC GACGACATCA 
GCGGCTTCAA CTTCACACGC AACTGCAGCT GTTAAGCAGC CCAGTAAACC TCATACGGAA 
GTATTCCTGA ATCCCCCACC ACCGCCATCG GCACAGCAAC AGTTGCAGAT CCACAGTTTA 
GTGCAAAATC AAGGTCAACA CCATCAGCAT TCTCACCCGC AGCAGTCGCA GCCGAGCCAG 
CAGAGTCACC AACAGCCACC GAGCTTGCAG ACCTACGGAT TAGGGGTCAG CAATAGCTGC 
AGTTCCTCCA ACAACCCCTC GACCTCGGGA TCTGGTCATC GTGCGAATCG TCAGACAAAC 
TCACCGTTTG GAGCACTGCT TAATGCTGCA GCGGCAGTGG CCTGCGCATC GCCGGCCTGT 
CCCACCACCT CATCTTCGGT ATTGAGGTTT GCAGAATCAC CGGTGGCACA CTACCTTGAG 
CGGGAAAATG GTGACTTCAT TGTCCAGGAG ACACCGAAGC ATATTGTAGA GTGTGTGGAG 
AAGGAAGACG GCGAGTTTTC CGTGATCGAA CGCATTTATC AATCCCCGCC GAGTGTTCTG 
CACATACACG ATGATGATGA GGATGACGAG GATGAAGAGG AGGAGGACGA GCATGATGCT 
CCGCCCGAGG AAGAAGACGG GGACGATGAG CGGGAGGAGA ACAGCCACAG CCGTAATATG 
AGCAAGACAA CGAAAAAGGC CCGTAAGACG AAGCCGAAGC GAGGCTCCAA GCGCATGTCG 
GACGAGGAGG AGTTCATGAG CCTTAGCAGC GGATGTGAGA GTGATTTGGA AAAGGAGGGT 
AAACAGGAGC AGCTAAATGC AGCGGCATCA CTACCACCTC CATTGTGCAC GGCTCCTTCT 
ACAAGTGCTG CGGCTGGAGC TTCCACCTCC AATCTGGCGG ACAATCATTC CAATTCCGCG 
TCCAACTCTG CCTCCAATTC GAACTCCACC GCCACTACAA AGTCCAAGAA GAATCGAATT 
ACCGTACTCA GCGATGTTCC GCTCAATATG AATGAGTACC TCGATTTGAT GGGCAACATC 
GTAGCCTCCA GTCGCATTGG GGCAAGTCGA CCGTTTGCCG CTGTGGCGCC CATACCACTC 
GTCAAGGTGG AGAAGGAAGA ACCTCTGGAT GATTACGACA ACGTGGCAAT GGCCGCTAAT 
GAAGAGCTGG AGCAAAAGCC CAGCCTTGAG CATGGCACCA CTAGCGTCAT TCGAATGGCA 
AGCACGGCTG CAGCATCGAC TTCTGCAACG GCGACCGAAA CATCTGCCGA GGATCTCACT 
CAGCCGCCGC CCATGAAAGT CGAGGTTGAG GCGACCACCA CATTGTTGCC GCAACGCTTT 
TCAACTCCCG CCCAGCATCG CGGGCCTAAG AAATTAGTCA TCAAACCAAA ACAAACAGCA 
CCAGCGACAG CGACGACAAC AAAAAAGACT GACACCCCAT CTTCCCCCAG CAACAACCCA 
TCCATATCAA GAGATCTGGT GCAAGTAAAG AGTGAGACAC TGGAGGCGCC AGCGACGAGC 
AGTAGCAGTA GCATTCTGGA GAAGCACCTA ACCACCAGCC GAAAACTTTT GCATCATCAC 
TCGGCGGTGA ACGATGACGA TGCCCGGGTG CTGCTGGAGT TTGCGAACTC GAAGCAGCCG 
CCGCCACTGG CCGCGTCGAG CACAACGTTT GTGGTTAATG CCAGTGTAGG CTTTCCCTCG 
GCTGGCTCAG TTTTCGCCAG CAGCAGTACC AGCTGTTCCA AAGCACCGGC CAAGCCGGGA 
GAGGAGTACA TCCTGCCGGA CAACAACATG GACGTGGATG AGATTGTGAT ATCCTCGTCG 
TCTTCCTACA CCTCCTCTGC AGCCCAGGTG CAGAACACGT TACAGCCGCC GGACTTTAAC 
TTTCTGTACC ACCAGGCCAC TACAACGGCC ACGACGGCCA CATACTTCAA TCCCTTTTCC 
AGGCAGCAGC AGCATCATCA TCAGCAGCAG CACGGCGTCA GCGATGCCAC CAATGCGGCT 
ACTTTGGCTT CGTCGTCCAG TAATTCGTTG GACCACGAGT CGATGAATGC TACATTCCGG 
GTGGCCAAGA CCCAATCGCT GCAGTCATGG TATCAGCAAG AGCCGGATCA CGATCCACAG 
AATCAGGGTG GTGCGGAATC GGGAACTGTC ACTCCCTCCA ATCAGAGCCA GGATGTGGTT 
AGCCACCAGC CAACAAACTT TAACGCCGCC CTAGCGGCCG TCGATTGCTG TAGCGTTGCC 
TTGGACGAGG ACGCAAAATA TCTTGATCTA GACGCCTGCA AGCGTGAGCA GCTGAGCAGC 
AGTAGCAATC TGCCCTCCGC TTCCGCGTCC ACAACGTCGT CGTCGTTCGC TGGCGCTGGC 
ACGGAGAGCA GTTTGGTGGG TGGTCTGAAT ATCCGAACCG ATGAAAAGAT GCCCGCCAAG 
GGTGAAATTT CCGAACAGGA AAGCAACTGC GACATCGAGA ACTCCTGGAG TGAGTCGGTT 
TATGGGGATA TTTCGCAGCG GTACTTCAGA AACCAGTTTT CTGGCGGCTA CCACCCTGAT 
TGGCGACAGG ACTATTATCC GGCTCAAGAT CTCAGCGCTC AGCTGAGAGA GCATAAACGT 
TTTGACTTTA ATGCTGTCGA TGATGAGGCT TCATCCAGCT CGAGAAGGAG CCATCTTACA 
GATCTCCCGA CAGCGGCCAC CTCCTCCGCA TGTACGTCAT CAGCTATGCT GGCGGGTCCA 
CCAATAGCTT CGACATCACG GGCTGCTGCT GAGGCGGCAG CGGAGGCGGC TGCTCTTGCC 
CAGCGGAAAC CGCAGCGCCG CAAGCTCTAC AAGTGTCCTC ACTGCGAGGC GATCTTCGAA 
AAGCTTAAGG AGCGTAATGC GCACATGATT GGCGAGCACA ACTACGTACG GCAAAATCGC 
CGTCTAATTT GCACTCAGCC GCAAATCAAT ACTTCGATGT TAACGCTACA GCAACAGATG 
ATAGTGCAGT CAGGTGAGGT TGAGGATTCC AAGGATGGCA TTGTGAAGAT AAAACAGGAG 
CAGTGCCAGG AAAAGCAGGA TGTTGAGCAA ATGCACGACC ATTCCGAACT ATTGGCTGAT 
GTCAAGGACT CTGAGCTGAT GGGCATCGGC GGTGATGGTG ACGGTGATGT AGATGACGAC 
ATTAAGCCAC CCAACCAGCT AGATGAAAAG CCCACGCTGC CGCCATTAGC CATGACGACC 
CCAGCGGCTA AATTAGCAGC TTTGTATCGA ATGCTCATCG CCTACAACGA ATCCAAGCTC 
GGACAGGAGC GCAACAATCT TAGCGAGCTG GAGCAGAAGG CTATGGAAAA ATCCATATTT 
CTCTGCTATG TCTGTCGTAC CGACTTCCCG TCCGTAAAGC TCTATGACGC CCATCTCACC 
GAGCATCCCG CTGAATGCTT TACGTGCGGC AAGAAGTTCT ACCGTTGGAA GAACTTCTCA 
TTGCACTTGA AGCGGCACTT GGGCTGGAAG GAGTTCGGAT GCTATGTCTG CGACAAGAAG 
TTCGTAGTGC GCAGCGCCTT GGTCGAACAC ATGCGGATGC ACACGGGGCA AACGCCACTC 
AAGTGCAAGA TATGCGGTAA AAAGTTCAAG AGATACTCGA ATCTGACGCA ACACCGCAAA 
CGCCACACCA AAATGATGGT GCGCAAGAAG GAATACGTGT GTCACTGCGG CGAAGTCTTG 
CCCTCGAAGG CTCGCTTCCT GTGGCACAAG GAAACGCACG ACCTGAAGCC GAAGTGCTGC 
CCGTACTGCT GCGATCGGTT TGTGCACGCC AACTCGCTAC GTCGACACAT ACGGTTGGCC 
CACTCTGATA AGTTTGACTA TGCCGAACCT ATGGAATGCC CTATGTGTAA GCAAATCTTC 
GCGAAGACGT CCATCAAGGC GCACATGGCG ACGCACTCAA CGGAGCCCCA ATACGATTGC 
GCCATTTGTA GCAAGAGCTT CTCCACCAAA TGGAATCTCA AGATCCACTC GTGGGTACAC 
GCCAACCGGA CGGCCAAGCC CTTTAAGTGC GAATACTGCC CAAAGGCGTT TGTGCGAGAG 
CTGGACTTCA AGAACCACAT CAACGCGCAC AAACAGATCA AACCGTACAC GTGCGAGTAC 
TGCGGCTGCA AGTTCATCCG CAAGTACAAT TACATGCGAC ACCGGCGCGA ACATCATGGC 
ACCAAGAAGT TCACCTGCGA CCAATGCGAT AAGTCTTTCC ATCGGCATTA CTATCTGATA 
GAGCACCGGC GAATGCATAC CGGGGAGCGT CCGTTCACGT GTACCATCTG CGGCAAGAGC 
TCCACAACGA AGACCAACCA CAACAAGCAT CTGAAGATCC ACCATTCGCG CGATCCGTTC 
ACGGTGGAGG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG31224 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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