Mps1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mps1
Entrez Gene ID	42126
Full Name	Monopolar spindle 1
Synonyms	Ald/Mps1,CG7643,Dmel\CG7643,Msp1,TKK,ald,dMps1,mps1
General protein information	Preferred Names Monopolar spindle 1 Names CG7643 gene product from transcript CG7643-RB CG7643-PA CG7643-PB Mps1-PA Mps1-PB altered disjunction monopolar spindle 1 monopolar spindles 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 90C1-90C1\|3-61 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275748.1	NP_001262677.1	monopolar spindle 1, isoform B
NM_142387.3	NP_650644.1	monopolar spindle 1, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	ald	NP_650644.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002075	XP_320972.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a model in which Polo controls Mps1-dependent BubR1 phosphorylation to promote Cdc20 kinetochore recruitment and sustained SAC function.
Title: Drosophila Polo regulates the spindle assembly checkpoint through Mps1-dependent BubR1 phosphorylation.

Kinetochore localized Mad2 and Cdc20 is itself insufficient for triggering the mitotic checkpoint when Mps1 is low in Drosophila melanogaster neuroblasts.
Title: Kinetochore localized Mad2 and Cdc20 is itself insufficient for triggering the mitotic checkpoint when Mps1 is low in Drosophila melanogaster neuroblasts.

Data indicate kinase activity seems to be required for Mps1 kinetochore localization.
Title: Spindle checkpoint-independent inhibition of mitotic chromosome segregation by Drosophila Mps1.

mps1 and polo are sequestered to collagenase-sensitive filaments in Drosophila prometaphase oocytes under hypoxic conditions
Title: Hypoxia transiently sequesters mps1 and polo to collagenase-sensitive filaments in Drosophila prometaphase oocytes.

oocytes lacking Ald/Mps1 protein enter anaphase I immediately upon completing spindle formation, in a fashion that does not allow sufficient time for nonexchange homologs to complete their normal partitioning to opposite half spindles
Title: The multiple roles of mps1 in Drosophila female meiosis.

The following Mps1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mps1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05252	NM_142387.3 Latest version!	Drosophila melanogaster monopolar spindle 1, transcript variant A (Mps1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa08441	NM_001275748.1 Latest version!	Drosophila melanogaster monopolar spindle 1, transcript variant B (Mps1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mps1 ( NM_142387.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650644.1 Drosophila melanogaster monopolar spindle 1, transcript variant A (Mps1), mRNA.

Price & Availability↑

CloneID	OFa05252
Clone ID Related Accession (Same CDS sequence)	NM_142387.3
Accession Version	NM_142387.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	monopolar spindle 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142387.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCACGC CTGTGCCCCG CCGCACCAAG GACATGATGG CACTGGGACT GGACTCCGAC 
TCCGAGGACG ACTTTAACAC GCCATACCGA CCACGACAAG CGGCGGCGGG AGAACGAAAA 
CAGCAGCCGG TGGCGTCTTT CCAAGTCCAA ACGAGGGGCA AGGAGAACGA ACCGCATCCC 
CTGCCCATAA ATATGCTACC TCGCCGAGTG TCCGAGTTGA CTATGATGGA TTCGGACAGT 
GACGAGGAGG ACATCAAGAG CAATCACAAC CTTAACTGCG CCATCCTAAA CGATTCCTTT 
GTGCTGAGCC CATCCCAAGA GTTAACAAAC AGCAACAGCA ACATAACCAC TCGTAGAACA 
CCAAGTGCCG CCCCCCTTAA GCAATCCGAT TCGAATCTCT CGTTCCTGGG AAGATTCAAC 
GACATGGGCA TCAATTGCAG CAGTCAAGGA TCCCCTGTTG CGAATTCGGA AAAACAGGTG 
GCCAAAAAGA CAGCACCCAC GCTTCAAGCT GCACCAAGTG CCACCGAAAG GCGCCCACTC 
CAAGAGACAG AGACGCCACT GCGTAATGAA TTACCCTCCA CCTCGAAGAC GAAGCCGGAT 
GCAGACTTCA TTACCCCCCA AGTACGAACA ATTGGTTCCA CACTGGCGGG AAAAAGTCGG 
AGTGCGGTGT CCAATGACTT CCGTGCGCAG AAAGTGCTCT TCCAAACACC TATGACCGTG 
AGTCGTGCTG CTCCAGTGGC CTCCGATAGC ATAAGCTTCT CCCTGTGCGA CACCATCACA 
GAAAGCCCGG ACATTCCGGA GCCTCCGAAA AAGGCAGAAC CACCAAAGAG TCAGCATCCT 
TCCAAAAAGT CCCTAGATCA TGTTTTCCGG GAGTCGGACA AGGATAACGT GCCAGACAAA 
GTGGATAATG TGGAACCAAA GGAGCTGGTA TCGATTCCCG CAGTTGCTGT ACCTCCAGAG 
CAGCCTTCCC ACAAGACATC TAATATTTTA AAAATCAAGA ATCATGAGTA TACGATTGAT 
AAGAAACTGG GCTGTGGCGG CTCCAGCTCT GTTTTTTTGG CACGCCGATC GGATTCCGGA 
AATGAGTTCG CCCTGAAGGT GGTGGATCTG CAGGCCGATC CACAAGTGGT GCAGGGATAT 
CTAAACGAAA CCAAACTTCT GGCAAAGCTA CAAGGGAACG TTTGTGTTGT GGCACTTTAC 
GATTATCAAC TTGTGCGCGA GGAGTCCAAG CTGTACATGG TGATGGAGAA AGGTGACTGC 
GACTTGAACA AGATCCTGCA GAGCTACACC ACCAACCTTC CTCTCTATAG CCTGATGAAC 
ATCCTGTACC AAATGCTGCA AGCGGTCAAC TACATTCACC AACACGGTGT CATCCATTCG 
GATCTCAAGC CGGCAAATTT CCTGATGGTC AGCGGCAGAC TGAAGTTGAT CGATTTTGGC 
ATAGCCAGCA ATATCGCTGT GGACTCGACG AGCATCATCA AATTCTCGCA GGCGGGCACC 
TTTAACTACA TTAGTCCGGA GGCGTTGACG GACACCTCCA CGGGGAATAG CCCGATGCGC 
AGAGCCGATC AGCCGAAGAT CAAGATCTCG ACCAAATCGG ACGTGTGGTC GCTGGGCTGC 
ATCCTTTACC TGCTACTCTA CCAAAAGACA CCGTTCGGCC ACATCCGAAA CGTCTATGCC 
AAGATGAGTG CAATCACCAC ACCGGGCACC AGCATCGAGT ATCCTGCCAT TCCGCCCTAC 
TATCCCATCA TGCTGGTTCA TATGGCCAAG AACTGCCTAC AGTTGAATCC CAAAAAGCGG 
CCGTCGTGCA CCGAACTACT ACAGTACCCA TTCCATATGA TCATTCCGCT GCAGAACCTG 
CAGATACCCA GCAGAACCGC CAACAGCAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650644.1
CDS	101..1993
Translation	MTTPVPRRTKDMMALGLDSDSEDDFNTPYRPRQAAAGERKQQPVASFQVQTRGKENEPHPLPINMLPRRVSELTMMDSDSDEEDIKSNHNLNCAILNDSFVLSPSQELTNSNSNITTRRTPSAAPLKQSDSNLSFLGRFNDMGINCSSQGSPVANSEKQVAKKTAPTLQAAPSATERRPLQETETPLRNELPSTSKTKPDADFITPQVRTIGSTLAGKSRSAVSNDFRAQKVLFQTPMTVSRAAPVASDSISFSLCDTITESPDIPEPPKKAEPPKSQHPSKKSLDHVFRESDKDNVPDKVDNVEPKELVSIPAVAVPPEQPSHKTSNILKIKNHEYTIDKKLGCGGSSSVFLARRSDSGNEFALKVVDLQADPQVVQGYLNETKLLAKLQGNVCVVALYDYQLVREESKLYMVMEKGDCDLNKILQSYTTNLPLYSLMNILYQMLQAVNYIHQHGVIHSDLKPANFLMVSGRLKLIDFGIASNIAVDSTSIIKFSQAGTFNYISPEALTDTSTGNSPMRRADQPKIKISTKSDVWSLGCILYLLLYQKTPFGHIRNVYAKMSAITTPGTSIEYPAIPPYYPIMLVHMAKNCLQLNPKKRPSCTELLQYPFHMIIPLQNLQIPSRTANSN

Target ORF information:

RefSeq Version	NM_142387.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster monopolar spindle 1, transcript variant A (Mps1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142387.3

ORF Insert Sequence:

ATGACCACGC CTGTGCCCCG CCGCACCAAG GACATGATGG CACTGGGACT GGACTCCGAC 
TCCGAGGACG ACTTTAACAC GCCATACCGA CCACGACAAG CGGCGGCGGG AGAACGAAAA 
CAGCAGCCGG TGGCGTCTTT CCAAGTCCAA ACGAGGGGCA AGGAGAACGA ACCGCATCCC 
CTGCCCATAA ATATGCTACC TCGCCGAGTG TCCGAGTTGA CTATGATGGA TTCGGACAGT 
GACGAGGAGG ACATCAAGAG CAATCACAAC CTTAACTGCG CCATCCTAAA CGATTCCTTT 
GTGCTGAGCC CATCCCAAGA GTTAACAAAC AGCAACAGCA ACATAACCAC TCGTAGAACA 
CCAAGTGCCG CCCCCCTTAA GCAATCCGAT TCGAATCTCT CGTTCCTGGG AAGATTCAAC 
GACATGGGCA TCAATTGCAG CAGTCAAGGA TCCCCTGTTG CGAATTCGGA AAAACAGGTG 
GCCAAAAAGA CAGCACCCAC GCTTCAAGCT GCACCAAGTG CCACCGAAAG GCGCCCACTC 
CAAGAGACAG AGACGCCACT GCGTAATGAA TTACCCTCCA CCTCGAAGAC GAAGCCGGAT 
GCAGACTTCA TTACCCCCCA AGTACGAACA ATTGGTTCCA CACTGGCGGG AAAAAGTCGG 
AGTGCGGTGT CCAATGACTT CCGTGCGCAG AAAGTGCTCT TCCAAACACC TATGACCGTG 
AGTCGTGCTG CTCCAGTGGC CTCCGATAGC ATAAGCTTCT CCCTGTGCGA CACCATCACA 
GAAAGCCCGG ACATTCCGGA GCCTCCGAAA AAGGCAGAAC CACCAAAGAG TCAGCATCCT 
TCCAAAAAGT CCCTAGATCA TGTTTTCCGG GAGTCGGACA AGGATAACGT GCCAGACAAA 
GTGGATAATG TGGAACCAAA GGAGCTGGTA TCGATTCCCG CAGTTGCTGT ACCTCCAGAG 
CAGCCTTCCC ACAAGACATC TAATATTTTA AAAATCAAGA ATCATGAGTA TACGATTGAT 
AAGAAACTGG GCTGTGGCGG CTCCAGCTCT GTTTTTTTGG CACGCCGATC GGATTCCGGA 
AATGAGTTCG CCCTGAAGGT GGTGGATCTG CAGGCCGATC CACAAGTGGT GCAGGGATAT 
CTAAACGAAA CCAAACTTCT GGCAAAGCTA CAAGGGAACG TTTGTGTTGT GGCACTTTAC 
GATTATCAAC TTGTGCGCGA GGAGTCCAAG CTGTACATGG TGATGGAGAA AGGTGACTGC 
GACTTGAACA AGATCCTGCA GAGCTACACC ACCAACCTTC CTCTCTATAG CCTGATGAAC 
ATCCTGTACC AAATGCTGCA AGCGGTCAAC TACATTCACC AACACGGTGT CATCCATTCG 
GATCTCAAGC CGGCAAATTT CCTGATGGTC AGCGGCAGAC TGAAGTTGAT CGATTTTGGC 
ATAGCCAGCA ATATCGCTGT GGACTCGACG AGCATCATCA AATTCTCGCA GGCGGGCACC 
TTTAACTACA TTAGTCCGGA GGCGTTGACG GACACCTCCA CGGGGAATAG CCCGATGCGC 
AGAGCCGATC AGCCGAAGAT CAAGATCTCG ACCAAATCGG ACGTGTGGTC GCTGGGCTGC 
ATCCTTTACC TGCTACTCTA CCAAAAGACA CCGTTCGGCC ACATCCGAAA CGTCTATGCC 
AAGATGAGTG CAATCACCAC ACCGGGCACC AGCATCGAGT ATCCTGCCAT TCCGCCCTAC 
TATCCCATCA TGCTGGTTCA TATGGCCAAG AACTGCCTAC AGTTGAATCC CAAAAAGCGG 
CCGTCGTGCA CCGAACTACT ACAGTACCCA TTCCATATGA TCATTCCGCT GCAGAACCTG 
CAGATACCCA GCAGAACCGC CAACAGCAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mps1 ( NM_001275748.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262677.1 Drosophila melanogaster monopolar spindle 1, transcript variant B (Mps1), mRNA.

Price & Availability↑

CloneID	OFa08441
Clone ID Related Accession (Same CDS sequence)	NM_001275748.1
Accession Version	NM_001275748.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	monopolar spindle 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACCACGC CTGTGCCCCG CCGCACCAAG GACATGATGG CACTGGGACT GGACTCCGAC 
TCCGAGGACG ACTTTAACAC GCCATACCGA CCACGACAAG CGGCGGCGGG AGAACGAAAA 
CAGCAGCCGG TGGCGTCTTT CCAAGTCCAA ACGAGGGGCA AGGAGAACGA ACCGCATCCC 
CTGCCCATAA ATATGCTACC TCGCCGAGTG TCCGAGTTGA CTATGATGGA TTCGGACAGT 
GACGAGGAGG ACATCAAGAG CAATCACAAC CTTAACTGCG CCATCCTAAA CGATTCCTTT 
GTGCTGAGCC CATCCCAAGA GTTAACAAAC AGCAACAGCA ACATAACCAC TCGTAGAACA 
CCAAGTGCCG CCCCCCTTAA GCAATCCGAT TCGAATCTCT CGTTCCTGGG AAGATTCAAC 
GACATGGGCA TCAATTGCAG CAGTCAAGGA TCCCCTGTTG CGAATTCGGA AAAACAGGTG 
GCCAAAAAGA CAGCACCCAC GCTTCAAGCT GCACCAAGTG CCACCGAAAG GCGCCCACTC 
CAAGAGACAG AGACGCCACT GCGTAATGAA TTACCCTCCA CCTCGAAGAC GAAGCCGGAT 
GCAGACTTCA TTACCCCCCA AGTACGAACA ATTGGTTCCA CACTGGCGGG AAAAAGTCGG 
AGTGCGGTGT CCAATGACTT CCGTGCGCAG AAAGTGCTCT TCCAAACACC TATGACCGTG 
AGTCGTGCTG CTCCAGTGGC CTCCGATAGC ATAAGCTTCT CCCTGTGCGA CACCATCACA 
GAAAGCCCGG ACATTCCGGA GCCTCCGAAA AAGGCAGAAC CACCAAAGAG TCAGCATCCT 
TCCAAAAAGT CCCTAGATCA TGTTTTCCGG GAGTCGGACA AGGATAACGT GCCAGACAAA 
GTGGATAATG TGGAACCAAA GGAGCTGGTA TCGATTCCCG CAGTTGCTGT ACCTCCAGAG 
CAGCCTTCCC ACAAGACATC TAATATTTTA AAAATCAAGA ATCATGAGTA TACGATTGAT 
AAGAAACTGG GCTGTGGCGG CTCCAGCTCT GTTTTTTTGG CACGCCGATC GGATTCCGGA 
AATGAGTTCG CCCTGAAGGT GGTGGATCTG CAGGCCGATC CACAAGTGGT GCAGGGATAT 
CTAAACGAAA CCAAACTTCT GGCAAAGCTA CAAGGGAACG TTTGTGTTGT GGCACTTTAC 
GATTATCAAC TTGTGCGCGA GGAGTCCAAG CTGTACATGG TGATGGAGAA AGGTGACTGC 
GACTTGAACA AGATCCTGCA GAGCTACACC ACCAACCTTC CTCTCTATAG CCTGATGAAC 
ATCCTGTACC AAATGCTGCA AGCGGTCAAC TACATTCACC AACACGGTGT CATCCATTCG 
GATCTCAAGC CGGCAAATTT CCTGATGGTC AGCGGCAGAC TGAAGTTGAT CGATTTTGGC 
ATAGCCAGCA ATATCGCTGT GGACTCGACG AGCATCATCA AATTCTCGCA GGCGGGCACC 
TTTAACTACA TTAGTCCGGA GGCGTTGACG GACACCTCCA CGGGGAATAG CCCGATGCGC 
AGAGCCGATC AGCCGAAGAT CAAGATCTCG ACCAAATCGG ACGTGTGGTC GCTGGGCTGC 
ATCCTTTACC TGCTACTCTA CCAAAAGACA CCGTTCGGCC ACATCCGAAA CGTCTATGCC 
AAGATGAGTG CAATCACCAC ACCGGGCACC AGCATCGAGT ATCCTGCCAT TCCGCCCTAC 
TATCCCATCA TGCTGGTTCA TTTTACAACT GTCTCTGCTC TATTTGCAGA TGGCCAAGAA 
CTGCCTACAG TTGAATCCCA AAAAGCGGCC GTCGTGCACC GAACTACTAC AGTACCCATT 
CCATATGATC ATTCCGCTGC AGAACCTGCA GATACCCAGC AGAACCGCCA ACAGCAATTA 
AGCACGAAAG CTCAGCTATT TATTAGTGAA ATGTCAAAGG TCAAATGTCT TTCTCATATG 
TGTGTGTATA TATTTAATTC TCATTTTATC GTAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262677.1
CDS	101..2119
Translation	MTTPVPRRTKDMMALGLDSDSEDDFNTPYRPRQAAAGERKQQPVASFQVQTRGKENEPHPLPINMLPRRVSELTMMDSDSDEEDIKSNHNLNCAILNDSFVLSPSQELTNSNSNITTRRTPSAAPLKQSDSNLSFLGRFNDMGINCSSQGSPVANSEKQVAKKTAPTLQAAPSATERRPLQETETPLRNELPSTSKTKPDADFITPQVRTIGSTLAGKSRSAVSNDFRAQKVLFQTPMTVSRAAPVASDSISFSLCDTITESPDIPEPPKKAEPPKSQHPSKKSLDHVFRESDKDNVPDKVDNVEPKELVSIPAVAVPPEQPSHKTSNILKIKNHEYTIDKKLGCGGSSSVFLARRSDSGNEFALKVVDLQADPQVVQGYLNETKLLAKLQGNVCVVALYDYQLVREESKLYMVMEKGDCDLNKILQSYTTNLPLYSLMNILYQMLQAVNYIHQHGVIHSDLKPANFLMVSGRLKLIDFGIASNIAVDSTSIIKFSQAGTFNYISPEALTDTSTGNSPMRRADQPKIKISTKSDVWSLGCILYLLLYQKTPFGHIRNVYAKMSAITTPGTSIEYPAIPPYYPIMLVHFTTVSALFADGQELPTVESQKAAVVHRTTTVPIPYDHSAAEPADTQQNRQQQLSTKAQLFISEMSKVKCLSHMCVYIFNSHFIVN

Target ORF information:

RefSeq Version	NM_001275748.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster monopolar spindle 1, transcript variant B (Mps1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275748.1

ORF Insert Sequence:

ATGACCACGC CTGTGCCCCG CCGCACCAAG GACATGATGG CACTGGGACT GGACTCCGAC 
TCCGAGGACG ACTTTAACAC GCCATACCGA CCACGACAAG CGGCGGCGGG AGAACGAAAA 
CAGCAGCCGG TGGCGTCTTT CCAAGTCCAA ACGAGGGGCA AGGAGAACGA ACCGCATCCC 
CTGCCCATAA ATATGCTACC TCGCCGAGTG TCCGAGTTGA CTATGATGGA TTCGGACAGT 
GACGAGGAGG ACATCAAGAG CAATCACAAC CTTAACTGCG CCATCCTAAA CGATTCCTTT 
GTGCTGAGCC CATCCCAAGA GTTAACAAAC AGCAACAGCA ACATAACCAC TCGTAGAACA 
CCAAGTGCCG CCCCCCTTAA GCAATCCGAT TCGAATCTCT CGTTCCTGGG AAGATTCAAC 
GACATGGGCA TCAATTGCAG CAGTCAAGGA TCCCCTGTTG CGAATTCGGA AAAACAGGTG 
GCCAAAAAGA CAGCACCCAC GCTTCAAGCT GCACCAAGTG CCACCGAAAG GCGCCCACTC 
CAAGAGACAG AGACGCCACT GCGTAATGAA TTACCCTCCA CCTCGAAGAC GAAGCCGGAT 
GCAGACTTCA TTACCCCCCA AGTACGAACA ATTGGTTCCA CACTGGCGGG AAAAAGTCGG 
AGTGCGGTGT CCAATGACTT CCGTGCGCAG AAAGTGCTCT TCCAAACACC TATGACCGTG 
AGTCGTGCTG CTCCAGTGGC CTCCGATAGC ATAAGCTTCT CCCTGTGCGA CACCATCACA 
GAAAGCCCGG ACATTCCGGA GCCTCCGAAA AAGGCAGAAC CACCAAAGAG TCAGCATCCT 
TCCAAAAAGT CCCTAGATCA TGTTTTCCGG GAGTCGGACA AGGATAACGT GCCAGACAAA 
GTGGATAATG TGGAACCAAA GGAGCTGGTA TCGATTCCCG CAGTTGCTGT ACCTCCAGAG 
CAGCCTTCCC ACAAGACATC TAATATTTTA AAAATCAAGA ATCATGAGTA TACGATTGAT 
AAGAAACTGG GCTGTGGCGG CTCCAGCTCT GTTTTTTTGG CACGCCGATC GGATTCCGGA 
AATGAGTTCG CCCTGAAGGT GGTGGATCTG CAGGCCGATC CACAAGTGGT GCAGGGATAT 
CTAAACGAAA CCAAACTTCT GGCAAAGCTA CAAGGGAACG TTTGTGTTGT GGCACTTTAC 
GATTATCAAC TTGTGCGCGA GGAGTCCAAG CTGTACATGG TGATGGAGAA AGGTGACTGC 
GACTTGAACA AGATCCTGCA GAGCTACACC ACCAACCTTC CTCTCTATAG CCTGATGAAC 
ATCCTGTACC AAATGCTGCA AGCGGTCAAC TACATTCACC AACACGGTGT CATCCATTCG 
GATCTCAAGC CGGCAAATTT CCTGATGGTC AGCGGCAGAC TGAAGTTGAT CGATTTTGGC 
ATAGCCAGCA ATATCGCTGT GGACTCGACG AGCATCATCA AATTCTCGCA GGCGGGCACC 
TTTAACTACA TTAGTCCGGA GGCGTTGACG GACACCTCCA CGGGGAATAG CCCGATGCGC 
AGAGCCGATC AGCCGAAGAT CAAGATCTCG ACCAAATCGG ACGTGTGGTC GCTGGGCTGC 
ATCCTTTACC TGCTACTCTA CCAAAAGACA CCGTTCGGCC ACATCCGAAA CGTCTATGCC 
AAGATGAGTG CAATCACCAC ACCGGGCACC AGCATCGAGT ATCCTGCCAT TCCGCCCTAC 
TATCCCATCA TGCTGGTTCA TTTTACAACT GTCTCTGCTC TATTTGCAGA TGGCCAAGAA 
CTGCCTACAG TTGAATCCCA AAAAGCGGCC GTCGTGCACC GAACTACTAC AGTACCCATT 
CCATATGATC ATTCCGCTGC AGAACCTGCA GATACCCAGC AGAACCGCCA ACAGCAATTA 
AGCACGAAAG CTCAGCTATT TATTAGTGAA ATGTCAAAGG TCAAATGTCT TTCTCATATG 
TGTGTGTATA TATTTAATTC TCATTTTATC GTAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mps1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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