Pak3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pak3
Entrez Gene ID	41995
Full Name	CG14895 gene product from transcript CG14895-RD
Synonyms	CG14895,D-Pak3,DPAK3,DPak3,DmPAK3,Dmel\CG14895,Dpak3,PAK-3,PAK3,PaK3,anon-WO0118547.285,dPAK3,dpak3,pak3
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 89B16-89B17

mRNA and Protein(s)

mRNA	Protein	Name
NM_142288.4	NP_650545.2	Pak3, isoform A
NM_169719.3	NP_732141.2	Pak3, isoform C
NM_001275709.1	NP_001262638.1	Pak3, isoform D

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001790	XP_321278.5
Drosophila melanogaster (fruit fly)	Pak3	NP_001262638.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study reports the role of the protein kinase Pak3 in maintaining apical-basal polarity in migrating border cell clusters during Drosophila oogenesis. Pak3 is enriched in border cells and downregulation of its function impedes border cell movement.
Title: Pak3 regulates apical-basal polarity in migrating border cells during Drosophila oogenesis.

The DPak3 is enriched at the site of fusion colocalizing with the F-actin focus within a podosome-like structure (PLS), and promotes actin filament assembly during PLS invasion.
Title: Group I PAKs function downstream of Rac to promote podosome invasion during myoblast fusion in vivo.

These results suggest that Pak3 specifically mediates Rac1 signaling in organizing actin and myosin during Drosophila epidermal wound healing.
Title: Requirement for Pak3 in Rac1-induced organization of actin and myosin during Drosophila larval wound healing.

pak3 therefore regulates synapse development in vivo, and when mutated, suppresses the synaptic defects that result from spastin loss.
Title: Loss of Drosophila melanogaster p21-activated kinase 3 suppresses defects in synapse structure and function caused by spastin mutations.

The following Pak3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pak3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05126	NM_142288.4 Latest version!	Drosophila melanogaster Pak3, transcript variant A (Pak3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05126	NM_169719.3 Latest version!	Drosophila melanogaster Pak3, transcript variant C (Pak3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa07930	NM_001275709.1 Latest version!	Drosophila melanogaster Pak3, transcript variant D (Pak3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pak3 ( NM_142288.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650545.2 Drosophila melanogaster Pak3, transcript variant A (Pak3), mRNA.

Price & Availability↑

CloneID	OFa05126
Clone ID Related Accession (Same CDS sequence)	NM_142288.4 , NM_169719.3
Accession Version	NM_142288.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Pak3, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142288.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCTTCA CCAAGTGGTT CAAGAAGAAG GGCGGAGATG GGGGATCGAT CTCAGAGATC 
GGTGCACCCA CAAACTTCCA ACGGCACTTC CATGTCTCGC GCAACCAGGA AACCGGCGAC 
CTGGAGGGGC TTCCAGCTCC ATGGGTGCGC CTAATGAACT CACAGATCAC TCGCGATGAG 
CAGGACAAGA ATCCAGATGC TGCCTACCAT GCCGTTAAGT ACTACAACTA TTCGATTAAG 
AAGAAAGAGA ACGAGGTCTT CAAGCCCTTC ATCACCGAAG ATGTGATCCA CGAGGAGTCC 
AAGGAGATAG AAAACTATGT CAACTACAAA AACAAGCACA AGTCTCAGGA TCCCGAAAAG 
TCCGACGACG ATGGCAGTTC GACGGCCACG GAGACGGAAA GTAGCAGCGG TTGTGGCTCC 
TCGGCAGGTA ATAGTAACAG CAGCAGCATC AACGACAGCA GCCAGCAGTC GACGGTGCCG 
CTGGACGCCC TAGAGGAGGT GTTCAAGGAG CTCAAGACCA ATCTGGAGCA CCGCGAAACG 
CTGAAGGCCG CACCGCAAGA GCCTCCACCA GTACCGCCCA AGAAATCGCC CCACACCATA 
CCGCCCAAAC CGCAAATCAA GCCCAAACCT CGTGTCACAC AGAAGTTCTC ACGCACTTCG 
GATATACGTC GAGATGAGGA CTCCGACAAC CAGCACAAGA TCAACACAGA CACAATCATC 
ATCAAGCCAG CCGTAGGGGC CCAGGATGCT GGAGCGGATG ATAATCCCGA CGAGACGATA 
CTGCGCCGCT CCAAGGAGAA GAGGGCCCAG AAAACCGATG CCGAAATCTA CGTTGAGCTG 
CGGGCAATTT GCAATTCAGA TGACCCAAGG GAACGCTACA AGACCACCCA GGAGGTGGGC 
AAGGGAGCTT CTGGCATAGT TTTCATAGCC GCCGATTTGC AGAACGAATC GCAGGTGGCC 
GTCAAGACCA TCGATATGAA GAACCAGTCC TCGAAGGATC TCATACTCAC AGAAATCCGA 
GTGCTCAAGG ACTTCAACCA CAAGAATCTG GTAAACTTTC TGGATGCCTA CCTTCTGGAG 
CCCGAGGACC AACTGTGGGT AGTCATGGAG TACATGGATG GCGGCCCATT GACTGACGTC 
GTTACCGAGA CTGTGATGAA GGAGCGCCAG ATTGCCTGCG TCTGCCGCGA GACACTCTAC 
GCTATTAGTT TTCTGCACGC TAAGGGCATT ATCCACCGGG ACATCAAGTC AGACAACGTG 
CTGCTGGGCA TGGATGGCAG TGTTAAGGTG ACGGACTTTG GGTTCTGCGC CAACATCGAG 
GGCGATGAGA AGCGCCAGAC AATGGTGGGA ACGCCGTACT GGATGGCGCC CGAAGTGGTC 
ACGCGCAAGA AGTACGGCAA GAAGGTGGAC ATCTGGTCTA TCGGCATCAT GGCCATCGAG 
ATGATCGAAG GCCAGCCACC CTACCTATAC GAGACTCCAC TCCGCGCCCT CTACCTGATC 
GCCGCCAACG GTCGGCCGGA TATCAAGAGC TGGGATAAGC TGAGTCCCAA CCTTCAAGAC 
TTTCTCGATC GCTGTCTCCA GGTGGAGGTG GACCGAAGGG CCACCGCCGA CGAGTTGCTC 
AGCCATCCGT TCCTCAACGA CTGCAGCGAG GTCAAGGCCC TCGTGCCCAA CATCAAGGCG 
GCCAAGAAGG TGCTCCGACG CAACGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650545.2
CDS	912..2621
Translation	MSFTKWFKKKGGDGGSISEIGAPTNFQRHFHVSRNQETGDLEGLPAPWVRLMNSQITRDEQDKNPDAAYHAVKYYNYSIKKKENEVFKPFITEDVIHEESKEIENYVNYKNKHKSQDPEKSDDDGSSTATETESSSGCGSSAGNSNSSSINDSSQQSTVPLDALEEVFKELKTNLEHRETLKAAPQEPPPVPPKKSPHTIPPKPQIKPKPRVTQKFSRTSDIRRDEDSDNQHKINTDTIIIKPAVGAQDAGADDNPDETILRRSKEKRAQKTDAEIYVELRAICNSDDPRERYKTTQEVGKGASGIVFIAADLQNESQVAVKTIDMKNQSSKDLILTEIRVLKDFNHKNLVNFLDAYLLEPEDQLWVVMEYMDGGPLTDVVTETVMKERQIACVCRETLYAISFLHAKGIIHRDIKSDNVLLGMDGSVKVTDFGFCANIEGDEKRQTMVGTPYWMAPEVVTRKKYGKKVDIWSIGIMAIEMIEGQPPYLYETPLRALYLIAANGRPDIKSWDKLSPNLQDFLDRCLQVEVDRRATADELLSHPFLNDCSEVKALVPNIKAAKKVLRRNV

Target ORF information:

RefSeq Version	NM_142288.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Pak3, transcript variant A (Pak3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142288.4

ORF Insert Sequence:

ATGAGCTTCA CCAAGTGGTT CAAGAAGAAG GGCGGAGATG GGGGATCGAT CTCAGAGATC 
GGTGCACCCA CAAACTTCCA ACGGCACTTC CATGTCTCGC GCAACCAGGA AACCGGCGAC 
CTGGAGGGGC TTCCAGCTCC ATGGGTGCGC CTAATGAACT CACAGATCAC TCGCGATGAG 
CAGGACAAGA ATCCAGATGC TGCCTACCAT GCCGTTAAGT ACTACAACTA TTCGATTAAG 
AAGAAAGAGA ACGAGGTCTT CAAGCCCTTC ATCACCGAAG ATGTGATCCA CGAGGAGTCC 
AAGGAGATAG AAAACTATGT CAACTACAAA AACAAGCACA AGTCTCAGGA TCCCGAAAAG 
TCCGACGACG ATGGCAGTTC GACGGCCACG GAGACGGAAA GTAGCAGCGG TTGTGGCTCC 
TCGGCAGGTA ATAGTAACAG CAGCAGCATC AACGACAGCA GCCAGCAGTC GACGGTGCCG 
CTGGACGCCC TAGAGGAGGT GTTCAAGGAG CTCAAGACCA ATCTGGAGCA CCGCGAAACG 
CTGAAGGCCG CACCGCAAGA GCCTCCACCA GTACCGCCCA AGAAATCGCC CCACACCATA 
CCGCCCAAAC CGCAAATCAA GCCCAAACCT CGTGTCACAC AGAAGTTCTC ACGCACTTCG 
GATATACGTC GAGATGAGGA CTCCGACAAC CAGCACAAGA TCAACACAGA CACAATCATC 
ATCAAGCCAG CCGTAGGGGC CCAGGATGCT GGAGCGGATG ATAATCCCGA CGAGACGATA 
CTGCGCCGCT CCAAGGAGAA GAGGGCCCAG AAAACCGATG CCGAAATCTA CGTTGAGCTG 
CGGGCAATTT GCAATTCAGA TGACCCAAGG GAACGCTACA AGACCACCCA GGAGGTGGGC 
AAGGGAGCTT CTGGCATAGT TTTCATAGCC GCCGATTTGC AGAACGAATC GCAGGTGGCC 
GTCAAGACCA TCGATATGAA GAACCAGTCC TCGAAGGATC TCATACTCAC AGAAATCCGA 
GTGCTCAAGG ACTTCAACCA CAAGAATCTG GTAAACTTTC TGGATGCCTA CCTTCTGGAG 
CCCGAGGACC AACTGTGGGT AGTCATGGAG TACATGGATG GCGGCCCATT GACTGACGTC 
GTTACCGAGA CTGTGATGAA GGAGCGCCAG ATTGCCTGCG TCTGCCGCGA GACACTCTAC 
GCTATTAGTT TTCTGCACGC TAAGGGCATT ATCCACCGGG ACATCAAGTC AGACAACGTG 
CTGCTGGGCA TGGATGGCAG TGTTAAGGTG ACGGACTTTG GGTTCTGCGC CAACATCGAG 
GGCGATGAGA AGCGCCAGAC AATGGTGGGA ACGCCGTACT GGATGGCGCC CGAAGTGGTC 
ACGCGCAAGA AGTACGGCAA GAAGGTGGAC ATCTGGTCTA TCGGCATCAT GGCCATCGAG 
ATGATCGAAG GCCAGCCACC CTACCTATAC GAGACTCCAC TCCGCGCCCT CTACCTGATC 
GCCGCCAACG GTCGGCCGGA TATCAAGAGC TGGGATAAGC TGAGTCCCAA CCTTCAAGAC 
TTTCTCGATC GCTGTCTCCA GGTGGAGGTG GACCGAAGGG CCACCGCCGA CGAGTTGCTC 
AGCCATCCGT TCCTCAACGA CTGCAGCGAG GTCAAGGCCC TCGTGCCCAA CATCAAGGCG 
GCCAAGAAGG TGCTCCGACG CAACGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pak3 ( NM_169719.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732141.2 Drosophila melanogaster Pak3, transcript variant C (Pak3), mRNA.

Price & Availability↑

CloneID	OFa05126
Clone ID Related Accession (Same CDS sequence)	NM_142288.4 , NM_169719.3
Accession Version	NM_169719.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Pak3, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169719.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCTTCA CCAAGTGGTT CAAGAAGAAG GGCGGAGATG GGGGATCGAT CTCAGAGATC 
GGTGCACCCA CAAACTTCCA ACGGCACTTC CATGTCTCGC GCAACCAGGA AACCGGCGAC 
CTGGAGGGGC TTCCAGCTCC ATGGGTGCGC CTAATGAACT CACAGATCAC TCGCGATGAG 
CAGGACAAGA ATCCAGATGC TGCCTACCAT GCCGTTAAGT ACTACAACTA TTCGATTAAG 
AAGAAAGAGA ACGAGGTCTT CAAGCCCTTC ATCACCGAAG ATGTGATCCA CGAGGAGTCC 
AAGGAGATAG AAAACTATGT CAACTACAAA AACAAGCACA AGTCTCAGGA TCCCGAAAAG 
TCCGACGACG ATGGCAGTTC GACGGCCACG GAGACGGAAA GTAGCAGCGG TTGTGGCTCC 
TCGGCAGGTA ATAGTAACAG CAGCAGCATC AACGACAGCA GCCAGCAGTC GACGGTGCCG 
CTGGACGCCC TAGAGGAGGT GTTCAAGGAG CTCAAGACCA ATCTGGAGCA CCGCGAAACG 
CTGAAGGCCG CACCGCAAGA GCCTCCACCA GTACCGCCCA AGAAATCGCC CCACACCATA 
CCGCCCAAAC CGCAAATCAA GCCCAAACCT CGTGTCACAC AGAAGTTCTC ACGCACTTCG 
GATATACGTC GAGATGAGGA CTCCGACAAC CAGCACAAGA TCAACACAGA CACAATCATC 
ATCAAGCCAG CCGTAGGGGC CCAGGATGCT GGAGCGGATG ATAATCCCGA CGAGACGATA 
CTGCGCCGCT CCAAGGAGAA GAGGGCCCAG AAAACCGATG CCGAAATCTA CGTTGAGCTG 
CGGGCAATTT GCAATTCAGA TGACCCAAGG GAACGCTACA AGACCACCCA GGAGGTGGGC 
AAGGGAGCTT CTGGCATAGT TTTCATAGCC GCCGATTTGC AGAACGAATC GCAGGTGGCC 
GTCAAGACCA TCGATATGAA GAACCAGTCC TCGAAGGATC TCATACTCAC AGAAATCCGA 
GTGCTCAAGG ACTTCAACCA CAAGAATCTG GTAAACTTTC TGGATGCCTA CCTTCTGGAG 
CCCGAGGACC AACTGTGGGT AGTCATGGAG TACATGGATG GCGGCCCATT GACTGACGTC 
GTTACCGAGA CTGTGATGAA GGAGCGCCAG ATTGCCTGCG TCTGCCGCGA GACACTCTAC 
GCTATTAGTT TTCTGCACGC TAAGGGCATT ATCCACCGGG ACATCAAGTC AGACAACGTG 
CTGCTGGGCA TGGATGGCAG TGTTAAGGTG ACGGACTTTG GGTTCTGCGC CAACATCGAG 
GGCGATGAGA AGCGCCAGAC AATGGTGGGA ACGCCGTACT GGATGGCGCC CGAAGTGGTC 
ACGCGCAAGA AGTACGGCAA GAAGGTGGAC ATCTGGTCTA TCGGCATCAT GGCCATCGAG 
ATGATCGAAG GCCAGCCACC CTACCTATAC GAGACTCCAC TCCGCGCCCT CTACCTGATC 
GCCGCCAACG GTCGGCCGGA TATCAAGAGC TGGGATAAGC TGAGTCCCAA CCTTCAAGAC 
TTTCTCGATC GCTGTCTCCA GGTGGAGGTG GACCGAAGGG CCACCGCCGA CGAGTTGCTC 
AGCCATCCGT TCCTCAACGA CTGCAGCGAG GTCAAGGCCC TCGTGCCCAA CATCAAGGCG 
GCCAAGAAGG TGCTCCGACG CAACGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732141.2
CDS	912..2621
Translation	MSFTKWFKKKGGDGGSISEIGAPTNFQRHFHVSRNQETGDLEGLPAPWVRLMNSQITRDEQDKNPDAAYHAVKYYNYSIKKKENEVFKPFITEDVIHEESKEIENYVNYKNKHKSQDPEKSDDDGSSTATETESSSGCGSSAGNSNSSSINDSSQQSTVPLDALEEVFKELKTNLEHRETLKAAPQEPPPVPPKKSPHTIPPKPQIKPKPRVTQKFSRTSDIRRDEDSDNQHKINTDTIIIKPAVGAQDAGADDNPDETILRRSKEKRAQKTDAEIYVELRAICNSDDPRERYKTTQEVGKGASGIVFIAADLQNESQVAVKTIDMKNQSSKDLILTEIRVLKDFNHKNLVNFLDAYLLEPEDQLWVVMEYMDGGPLTDVVTETVMKERQIACVCRETLYAISFLHAKGIIHRDIKSDNVLLGMDGSVKVTDFGFCANIEGDEKRQTMVGTPYWMAPEVVTRKKYGKKVDIWSIGIMAIEMIEGQPPYLYETPLRALYLIAANGRPDIKSWDKLSPNLQDFLDRCLQVEVDRRATADELLSHPFLNDCSEVKALVPNIKAAKKVLRRNV

Target ORF information:

RefSeq Version	NM_169719.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Pak3, transcript variant C (Pak3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169719.3

ORF Insert Sequence:

ATGAGCTTCA CCAAGTGGTT CAAGAAGAAG GGCGGAGATG GGGGATCGAT CTCAGAGATC 
GGTGCACCCA CAAACTTCCA ACGGCACTTC CATGTCTCGC GCAACCAGGA AACCGGCGAC 
CTGGAGGGGC TTCCAGCTCC ATGGGTGCGC CTAATGAACT CACAGATCAC TCGCGATGAG 
CAGGACAAGA ATCCAGATGC TGCCTACCAT GCCGTTAAGT ACTACAACTA TTCGATTAAG 
AAGAAAGAGA ACGAGGTCTT CAAGCCCTTC ATCACCGAAG ATGTGATCCA CGAGGAGTCC 
AAGGAGATAG AAAACTATGT CAACTACAAA AACAAGCACA AGTCTCAGGA TCCCGAAAAG 
TCCGACGACG ATGGCAGTTC GACGGCCACG GAGACGGAAA GTAGCAGCGG TTGTGGCTCC 
TCGGCAGGTA ATAGTAACAG CAGCAGCATC AACGACAGCA GCCAGCAGTC GACGGTGCCG 
CTGGACGCCC TAGAGGAGGT GTTCAAGGAG CTCAAGACCA ATCTGGAGCA CCGCGAAACG 
CTGAAGGCCG CACCGCAAGA GCCTCCACCA GTACCGCCCA AGAAATCGCC CCACACCATA 
CCGCCCAAAC CGCAAATCAA GCCCAAACCT CGTGTCACAC AGAAGTTCTC ACGCACTTCG 
GATATACGTC GAGATGAGGA CTCCGACAAC CAGCACAAGA TCAACACAGA CACAATCATC 
ATCAAGCCAG CCGTAGGGGC CCAGGATGCT GGAGCGGATG ATAATCCCGA CGAGACGATA 
CTGCGCCGCT CCAAGGAGAA GAGGGCCCAG AAAACCGATG CCGAAATCTA CGTTGAGCTG 
CGGGCAATTT GCAATTCAGA TGACCCAAGG GAACGCTACA AGACCACCCA GGAGGTGGGC 
AAGGGAGCTT CTGGCATAGT TTTCATAGCC GCCGATTTGC AGAACGAATC GCAGGTGGCC 
GTCAAGACCA TCGATATGAA GAACCAGTCC TCGAAGGATC TCATACTCAC AGAAATCCGA 
GTGCTCAAGG ACTTCAACCA CAAGAATCTG GTAAACTTTC TGGATGCCTA CCTTCTGGAG 
CCCGAGGACC AACTGTGGGT AGTCATGGAG TACATGGATG GCGGCCCATT GACTGACGTC 
GTTACCGAGA CTGTGATGAA GGAGCGCCAG ATTGCCTGCG TCTGCCGCGA GACACTCTAC 
GCTATTAGTT TTCTGCACGC TAAGGGCATT ATCCACCGGG ACATCAAGTC AGACAACGTG 
CTGCTGGGCA TGGATGGCAG TGTTAAGGTG ACGGACTTTG GGTTCTGCGC CAACATCGAG 
GGCGATGAGA AGCGCCAGAC AATGGTGGGA ACGCCGTACT GGATGGCGCC CGAAGTGGTC 
ACGCGCAAGA AGTACGGCAA GAAGGTGGAC ATCTGGTCTA TCGGCATCAT GGCCATCGAG 
ATGATCGAAG GCCAGCCACC CTACCTATAC GAGACTCCAC TCCGCGCCCT CTACCTGATC 
GCCGCCAACG GTCGGCCGGA TATCAAGAGC TGGGATAAGC TGAGTCCCAA CCTTCAAGAC 
TTTCTCGATC GCTGTCTCCA GGTGGAGGTG GACCGAAGGG CCACCGCCGA CGAGTTGCTC 
AGCCATCCGT TCCTCAACGA CTGCAGCGAG GTCAAGGCCC TCGTGCCCAA CATCAAGGCG 
GCCAAGAAGG TGCTCCGACG CAACGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pak3 ( NM_001275709.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262638.1 Drosophila melanogaster Pak3, transcript variant D (Pak3), mRNA.

Price & Availability↑

CloneID	OFa07930
Clone ID Related Accession (Same CDS sequence)	NM_001275709.1
Accession Version	NM_001275709.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Pak3, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCTTCA CCAAGTGGTT CAAGAAGAAG GGCGGAGATG GGGGATCGAT CTCAGAGATC 
GGTGCACCCA CAAACTTCCA ACGGCACTTC CATGTCTCGC GCAACCAGGA AACCGGCGAC 
CTGGAGGGGC TTCCAGCTCC ATGGGTGCGC CTAATGAACT CACAGATCAC TCGCGATGAG 
CAGGACAAGA ATCCAGATGC TGCCTACCAT GCCGTTAAGT ACTACAACTA TTCGATTAAG 
AAGAAAGAGA ACGAGGTCTT CAAGCCCTTC ATCACCGAAG ATGTGATCCA CGAGGAGTCC 
AAGGAGATAG AAAACTATGT CAACTACAAA AACAAGCACA AGTCTCAGGA TCCCGAAAAG 
TCCGACGACG ATGGCAGTTC GACGGCCACG GAGACGGAAA GTAGCAGCGG TTGTGGCTCC 
TCGGCAGGTA ATAGTAACAG CAGCAGCATC AACGACAGCA GCCAGCAGTC GACGGTGCCG 
CTGGACGCCC TAGAGGAGGT GTTCAAGGAG CTCAAGACCA ATCTGGAGCA CCGCGAAACG 
CTGAAGGCCG CACCGCAAGA GCCTCCACCA GTACCGCCCA AGAAATCGCC CCACACCATA 
CCGCCCAAAC CGCAAATCAA GCCCAAACCT CGTGTCACAC AGAAGTTCTC ACGCACTTCG 
GATATACGTC GAGATGAGGA CTCCGACAAC CAGCACAAGA TCAACACAGA CACAATCATC 
ATCAAGCCAG CCGTAGGGGC CCAGGATGCT GGAGCGGATG ATAATCCCGA CGAGACGATA 
CTGCGCCGCT CCAAGGAGAA GAGGGCCCAG AAAACCGATG CCGAAATCTA CGTTGAGCTG 
CGGGCAATTT GCAATTCAGA TGACCCAAGG GAACGCTACA AGACCACCCA GGAGGTGGGC 
AAGGGAGCTT CTGGCATAGT TTTCATAGCC GCCGATTTGC AGAACGAATC GCAGGTGGCC 
GTCAAGACCA TCGATATGAA GAACCAGTCC TCGAAGGATC TCATACTCAC AGAAATCCGA 
GTGCTCAAGG ACTTCAACCA CAAGAATCTG GTAAACTTTC TGGATGCCTA CCTTCTGGAG 
CCCGAGGACC AACTGTGGGT AGTCATGGAG TACATGGATG GCGGCCCATT GACTGACGTC 
GTTACCGAGA CTGTGATGAA GGAGCGCCAG ATTGCCTGCG TCTGCCGCGA GACACTCTAC 
GCTATTAGTT TTCTGCACGC TAAGGGCATT ATCCACCGGG ACATCAAGTC AGACAACGTG 
CTGCTGGGCA TGGATGGCAG TGTTAAGGTG ACGGACTTTG GGTTCTGCGC CAACATCGAG 
GGCGATGAGA AGCGCCAGAC AATGGTGGGA ACGCCGTACT GGATGGCGCC CGAAGTGGTC 
ACGCGCAAGA AGTACGGCAA GAAGGTGGAC ATCTGGTCTA TCGGCATCAT GGCCATCGAG 
ATGATCGAAG GCCAGCCACC CTACCTATAC GAGACTCCAC TCCGCGCCCT CTACCTGATC 
GCCGCCAACG GTCGGCCGGA TATCAAGAGC TGGGATAAGC TGAGTCCCAA CCTTCAAGAC 
TTTCTCGATC GCTGTCTCCA GGTGGAGGTG GACCGAAGGG CCACCGCCGA CGAGTTGCTC 
AGCCATCCGT TCCTCAACGA CTGCAGCGAG GTCAAGGCCC TCGTGCCCAA CATCAAGGCG 
GCCAAGAAGG TGCTCCGACG CAACGTATAG TTGCTATGCA ATCACCGGAC GGCCAGGCTG 
CTCTACGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262638.1
CDS	912..2663
Translation	MSFTKWFKKKGGDGGSISEIGAPTNFQRHFHVSRNQETGDLEGLPAPWVRLMNSQITRDEQDKNPDAAYHAVKYYNYSIKKKENEVFKPFITEDVIHEESKEIENYVNYKNKHKSQDPEKSDDDGSSTATETESSSGCGSSAGNSNSSSINDSSQQSTVPLDALEEVFKELKTNLEHRETLKAAPQEPPPVPPKKSPHTIPPKPQIKPKPRVTQKFSRTSDIRRDEDSDNQHKINTDTIIIKPAVGAQDAGADDNPDETILRRSKEKRAQKTDAEIYVELRAICNSDDPRERYKTTQEVGKGASGIVFIAADLQNESQVAVKTIDMKNQSSKDLILTEIRVLKDFNHKNLVNFLDAYLLEPEDQLWVVMEYMDGGPLTDVVTETVMKERQIACVCRETLYAISFLHAKGIIHRDIKSDNVLLGMDGSVKVTDFGFCANIEGDEKRQTMVGTPYWMAPEVVTRKKYGKKVDIWSIGIMAIEMIEGQPPYLYETPLRALYLIAANGRPDIKSWDKLSPNLQDFLDRCLQVEVDRRATADELLSHPFLNDCSEVKALVPNIKAAKKVLRRNV*LLCNHRTARLLYA

Target ORF information:

RefSeq Version	NM_001275709.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Pak3, transcript variant D (Pak3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275709.1

ORF Insert Sequence:

ATGAGCTTCA CCAAGTGGTT CAAGAAGAAG GGCGGAGATG GGGGATCGAT CTCAGAGATC 
GGTGCACCCA CAAACTTCCA ACGGCACTTC CATGTCTCGC GCAACCAGGA AACCGGCGAC 
CTGGAGGGGC TTCCAGCTCC ATGGGTGCGC CTAATGAACT CACAGATCAC TCGCGATGAG 
CAGGACAAGA ATCCAGATGC TGCCTACCAT GCCGTTAAGT ACTACAACTA TTCGATTAAG 
AAGAAAGAGA ACGAGGTCTT CAAGCCCTTC ATCACCGAAG ATGTGATCCA CGAGGAGTCC 
AAGGAGATAG AAAACTATGT CAACTACAAA AACAAGCACA AGTCTCAGGA TCCCGAAAAG 
TCCGACGACG ATGGCAGTTC GACGGCCACG GAGACGGAAA GTAGCAGCGG TTGTGGCTCC 
TCGGCAGGTA ATAGTAACAG CAGCAGCATC AACGACAGCA GCCAGCAGTC GACGGTGCCG 
CTGGACGCCC TAGAGGAGGT GTTCAAGGAG CTCAAGACCA ATCTGGAGCA CCGCGAAACG 
CTGAAGGCCG CACCGCAAGA GCCTCCACCA GTACCGCCCA AGAAATCGCC CCACACCATA 
CCGCCCAAAC CGCAAATCAA GCCCAAACCT CGTGTCACAC AGAAGTTCTC ACGCACTTCG 
GATATACGTC GAGATGAGGA CTCCGACAAC CAGCACAAGA TCAACACAGA CACAATCATC 
ATCAAGCCAG CCGTAGGGGC CCAGGATGCT GGAGCGGATG ATAATCCCGA CGAGACGATA 
CTGCGCCGCT CCAAGGAGAA GAGGGCCCAG AAAACCGATG CCGAAATCTA CGTTGAGCTG 
CGGGCAATTT GCAATTCAGA TGACCCAAGG GAACGCTACA AGACCACCCA GGAGGTGGGC 
AAGGGAGCTT CTGGCATAGT TTTCATAGCC GCCGATTTGC AGAACGAATC GCAGGTGGCC 
GTCAAGACCA TCGATATGAA GAACCAGTCC TCGAAGGATC TCATACTCAC AGAAATCCGA 
GTGCTCAAGG ACTTCAACCA CAAGAATCTG GTAAACTTTC TGGATGCCTA CCTTCTGGAG 
CCCGAGGACC AACTGTGGGT AGTCATGGAG TACATGGATG GCGGCCCATT GACTGACGTC 
GTTACCGAGA CTGTGATGAA GGAGCGCCAG ATTGCCTGCG TCTGCCGCGA GACACTCTAC 
GCTATTAGTT TTCTGCACGC TAAGGGCATT ATCCACCGGG ACATCAAGTC AGACAACGTG 
CTGCTGGGCA TGGATGGCAG TGTTAAGGTG ACGGACTTTG GGTTCTGCGC CAACATCGAG 
GGCGATGAGA AGCGCCAGAC AATGGTGGGA ACGCCGTACT GGATGGCGCC CGAAGTGGTC 
ACGCGCAAGA AGTACGGCAA GAAGGTGGAC ATCTGGTCTA TCGGCATCAT GGCCATCGAG 
ATGATCGAAG GCCAGCCACC CTACCTATAC GAGACTCCAC TCCGCGCCCT CTACCTGATC 
GCCGCCAACG GTCGGCCGGA TATCAAGAGC TGGGATAAGC TGAGTCCCAA CCTTCAAGAC 
TTTCTCGATC GCTGTCTCCA GGTGGAGGTG GACCGAAGGG CCACCGCCGA CGAGTTGCTC 
AGCCATCCGT TCCTCAACGA CTGCAGCGAG GTCAAGGCCC TCGTGCCCAA CATCAAGGCG 
GCCAAGAAGG TGCTCCGACG CAACGTATAG TTGCTATGCA ATCACCGGAC GGCCAGGCTG 
CTCTACGCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pak3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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