asun cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	asun
Entrez Gene ID	41971
Full Name	asunder
Synonyms	ASUN,Asu,CG6814,Dmel\CG6814,IntS13,Mat89Bb
General protein information	Preferred Names asunder Names CG6814 gene product from transcript CG6814-RA CG6814-PA asun-PA integrator 13 maternal transcript 89Bb
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 89B9-89B9

mRNA and Protein(s)

mRNA	Protein	Name
NM_079655.4	NP_524379.2	asunder

Pathways from BioSystems

Homologies

Homo sapiens (human)	ASUN	NP_060634.2
	LOC708806	XP_002798559.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002295	XP_307882.4
Xenopus tropicalis (tropical clawed frog)	asun	NP_001004984.1
Bos taurus (cattle)	ASUN	NP_001039964.1
Gallus gallus (chicken)	C1H12ORF11	XP_004938067.1
Mus musculus (house mouse)	Asun	NP_620096.2
Drosophila melanogaster (fruit fly)	asun	NP_524379.2
Caenorhabditis elegans (roundworm)	CELE_R02D3.4	NP_499881.1
Pan troglodytes (chimpanzee)	ASUN	XP_520806.2
Rattus norvegicus (Norway rat)	Asun	NP_001103078.1
Canis lupus familiaris (dog)	ASUN	NP_001184243.1
Danio rerio (zebrafish)	asun	NP_956177.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

asun is a critical regulator of dynein localization and dynein-mediated processes during Drosophila oogenesis
Title: asunder is required for dynein localization and dorsal fate determination during Drosophila oogenesis.

ASUN is identified as a functional component of Integrator (INT), a multisubunit complex required for 3'-end processing of small nuclear RNAs.
Title: Nuclear-localized Asunder regulates cytoplasmic dynein localization via its role in the integrator complex.

Functional and biochemical experiments revealed that Asunder and CG4785 are additional core members of the Integrator complex.
Title: An RNAi screen identifies additional members of the Drosophila Integrator complex and a requirement for cyclin C/Cdk8 in snRNA 3'-end formation.

asunder(previously Mat89Bb)required for male fertility; asun spermatocytes & spermatids exhibit reduction of perinuclear dynein-dynactin; model proposed in which asun coordinates spermatogenesis by promoting dynein-dynactin recruitment to nuclear surface
Title: Asunder is a critical regulator of dynein-dynactin localization during Drosophila spermatogenesis.

The following asun gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the asun cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05102	NM_079655.4 Latest version!	Drosophila melanogaster asunder (asun), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

asun ( NM_079655.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524379.2 Drosophila melanogaster asunder (asun), mRNA.

Price & Availability↑

CloneID	OFa05102
Clone ID Related Accession (Same CDS sequence)	NM_079655.4
Accession Version	NM_079655.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	asunder
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_079655.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCGAAC GCAACCAGAA GACCATCTTT GTGCTGGACC ACACCCGATA CTTTAGCATC 
GCCAGCGAGG AGTACATCTC GATGGACTTC CTAAAGGGAA AACCATCTGC AGACGGCGGC 
GCAACAGGAG CGGCGGGAAA TGCAACCGGC AGCGGTGGAT CCCAATTCAG CAAGAGCCTG 
TGGACATGCG CCTGCGAATC CTCCATCGAG TACTGCCGCG TGGTCTGGGA TCTCTTTCCC 
GGCAAAAAGC ATGTGCGGTT TATTGTCTCG GACACGGCGG CGCATATCGT GAACACCTGG 
AGGCCCAGCA CGCAAAATAT GGCGCACGTG ATGAACGCAA TGCTAATTGT GGGCGTGCCG 
TCGCGCAACG TACCCACATC TTCTGACTAC TCGGTAATCC ATGGCCTGCG GGCCGCCATC 
GAAGCGCTGG CGGAGCCCAC CGATGAGCAG TTGGCGGCAA TGGCCGACTT TGGGACCGAC 
GAACTGCCGC GCATTCCCAA CAAGGGTCGC GTCATCTGCA TAACTTCAGC GCGTGACAAT 
ACCAGCATGA AAAGCCTGGA GGACATCTTT AACACCGTGC TGGTGCAGCA GAACACCCTA 
GCAGCTCCGC CCTCCAAGAA GGGACTGGTC ATCGACCATT GCCACCTAGT TATCCTCAAC 
ATTGTGCCGC TGGGCGTTGA ATCGTTGGTT ACCAATCGCA GCCTGCTCAA GATATCGCCG 
CTGCTTGATG TCGAGATCCA CACAGTCAGT GCCCCGGATA TTTCCTATAA GCTGACGCAC 
CTCATACTGA ACCATTACGA CCTAGCTAGT ACCACGGTGA CTAACATACC CATGAAAGAG 
GAGCAGAACG CCAACTCCAG CGCCAACTAC GATGTGGAAA TCCTGCACAG CCGAAGGGCC 
CACTCCATCA CCTGTGGCCC AGATTTCAGT CTACCCACGA GCATCAAGCA AGGTGCCACA 
TACGAAACAG TGACTCTTAA GTGGTGCACA CCGCGCGGTT GCGGCTCTGC AGATCTACAG 
CCCTGTCTGG GTCAGTTCCT CGTGACACCG GTGGACGTCA CCTCGCGGCC CAGTTCTTGC 
TTGATTAACT TTCTGCTTAA CGGACGTTCA GTCTTGCTGG AAATGCCGCG CAAGACCGGC 
TCAAAGGCCA CCAGCCATAT GCTTTCTGCA CGCGGTGGCG AGATCTTTGT GCACTCCCTG 
TGCATCACGC GCTCCTGCAT GGATGAGGCT CCATCAATTA CTGATGGCCC CGGAGGACGT 
GTCTCGGACT ATCGCACCGC CGAACTTGGT CAACTGATCA AGATGTCGCG CGTGGTTCCG 
CTCAAGGTTA AGGATCCATC TGCTCCGCCC TTGACGCGCC GGTTGCCTCG CTACTTCCCC 
CTGACCACCA GCTCTTCGAT TCTGTTCCAT CTACAGCGAC ACATCAGTTG GCTTCCGCAT 
TTTCTCCATC TTTTAGTGAA GGAAGACATG GATAAGCAGG ACGAGGTGCG GTGCCAGCAG 
CACATTCACG AGCTGTACAA AAGCGCCTCG CGTGGCGATG TCCTACCATT TACCCACACC 
AATGGAGCAA GACTAAAGCT TTCCAAGGCC AAAGACCAGT ACCGCTTGCT GTACAGGGAA 
CTGGAGCAAT TAATTCAACT AAATGCCACC ACAATGCACC ATAAAAATCT GCTGGAAAGC 
CTACAGAGTT TGCGAGCCGC ATACGGCGAT GCCCCGCTGA AGTCGGAACC TGGAGCAAGT 
CTCCTGCGCA CCTACACGGA ATCCCCACTC TCTCCCGAAC GGCTGGAGCC CATCAGTAGC 
GTCGGTGCTA GTGGCAGCAG CAGCTCCAAT AGCCTTCTCA AGGCGAGCAA GCGTCGCATG 
TCTAGTTGTG GGCAAAGATC CCTGCTAGAT ATTATATCCT CAGCAGAGCG AAGCCAGTCC 
AACAAACGAT TGGATTTCTC GGGACGCCTT TGCACTCCGT TGGGTCAAGT GGCCAAGCTG 
TATCCGGATT TTGGTACCAA GGACAAAGAC ACAGTTACGA CGGGGGCCAG TATTACGCCC 
AATGTTAAGG AGGAATCCGT ACGTAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524379.2
CDS	83..2152
Translation	MFERNQKTIFVLDHTRYFSIASEEYISMDFLKGKPSADGGATGAAGNATGSGGSQFSKSLWTCACESSIEYCRVVWDLFPGKKHVRFIVSDTAAHIVNTWRPSTQNMAHVMNAMLIVGVPSRNVPTSSDYSVIHGLRAAIEALAEPTDEQLAAMADFGTDELPRIPNKGRVICITSARDNTSMKSLEDIFNTVLVQQNTLAAPPSKKGLVIDHCHLVILNIVPLGVESLVTNRSLLKISPLLDVEIHTVSAPDISYKLTHLILNHYDLASTTVTNIPMKEEQNANSSANYDVEILHSRRAHSITCGPDFSLPTSIKQGATYETVTLKWCTPRGCGSADLQPCLGQFLVTPVDVTSRPSSCLINFLLNGRSVLLEMPRKTGSKATSHMLSARGGEIFVHSLCITRSCMDEAPSITDGPGGRVSDYRTAELGQLIKMSRVVPLKVKDPSAPPLTRRLPRYFPLTTSSSILFHLQRHISWLPHFLHLLVKEDMDKQDEVRCQQHIHELYKSASRGDVLPFTHTNGARLKLSKAKDQYRLLYRELEQLIQLNATTMHHKNLLESLQSLRAAYGDAPLKSEPGASLLRTYTESPLSPERLEPISSVGASGSSSSNSLLKASKRRMSSCGQRSLLDIISSAERSQSNKRLDFSGRLCTPLGQVAKLYPDFGTKDKDTVTTGASITPNVKEESVRS

Target ORF information:

RefSeq Version	NM_079655.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster asunder (asun), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079655.4

ORF Insert Sequence:

ATGTTCGAAC GCAACCAGAA GACCATCTTT GTGCTGGACC ACACCCGATA CTTTAGCATC 
GCCAGCGAGG AGTACATCTC GATGGACTTC CTAAAGGGAA AACCATCTGC AGACGGCGGC 
GCAACAGGAG CGGCGGGAAA TGCAACCGGC AGCGGTGGAT CCCAATTCAG CAAGAGCCTG 
TGGACATGCG CCTGCGAATC CTCCATCGAG TACTGCCGCG TGGTCTGGGA TCTCTTTCCC 
GGCAAAAAGC ATGTGCGGTT TATTGTCTCG GACACGGCGG CGCATATCGT GAACACCTGG 
AGGCCCAGCA CGCAAAATAT GGCGCACGTG ATGAACGCAA TGCTAATTGT GGGCGTGCCG 
TCGCGCAACG TACCCACATC TTCTGACTAC TCGGTAATCC ATGGCCTGCG GGCCGCCATC 
GAAGCGCTGG CGGAGCCCAC CGATGAGCAG TTGGCGGCAA TGGCCGACTT TGGGACCGAC 
GAACTGCCGC GCATTCCCAA CAAGGGTCGC GTCATCTGCA TAACTTCAGC GCGTGACAAT 
ACCAGCATGA AAAGCCTGGA GGACATCTTT AACACCGTGC TGGTGCAGCA GAACACCCTA 
GCAGCTCCGC CCTCCAAGAA GGGACTGGTC ATCGACCATT GCCACCTAGT TATCCTCAAC 
ATTGTGCCGC TGGGCGTTGA ATCGTTGGTT ACCAATCGCA GCCTGCTCAA GATATCGCCG 
CTGCTTGATG TCGAGATCCA CACAGTCAGT GCCCCGGATA TTTCCTATAA GCTGACGCAC 
CTCATACTGA ACCATTACGA CCTAGCTAGT ACCACGGTGA CTAACATACC CATGAAAGAG 
GAGCAGAACG CCAACTCCAG CGCCAACTAC GATGTGGAAA TCCTGCACAG CCGAAGGGCC 
CACTCCATCA CCTGTGGCCC AGATTTCAGT CTACCCACGA GCATCAAGCA AGGTGCCACA 
TACGAAACAG TGACTCTTAA GTGGTGCACA CCGCGCGGTT GCGGCTCTGC AGATCTACAG 
CCCTGTCTGG GTCAGTTCCT CGTGACACCG GTGGACGTCA CCTCGCGGCC CAGTTCTTGC 
TTGATTAACT TTCTGCTTAA CGGACGTTCA GTCTTGCTGG AAATGCCGCG CAAGACCGGC 
TCAAAGGCCA CCAGCCATAT GCTTTCTGCA CGCGGTGGCG AGATCTTTGT GCACTCCCTG 
TGCATCACGC GCTCCTGCAT GGATGAGGCT CCATCAATTA CTGATGGCCC CGGAGGACGT 
GTCTCGGACT ATCGCACCGC CGAACTTGGT CAACTGATCA AGATGTCGCG CGTGGTTCCG 
CTCAAGGTTA AGGATCCATC TGCTCCGCCC TTGACGCGCC GGTTGCCTCG CTACTTCCCC 
CTGACCACCA GCTCTTCGAT TCTGTTCCAT CTACAGCGAC ACATCAGTTG GCTTCCGCAT 
TTTCTCCATC TTTTAGTGAA GGAAGACATG GATAAGCAGG ACGAGGTGCG GTGCCAGCAG 
CACATTCACG AGCTGTACAA AAGCGCCTCG CGTGGCGATG TCCTACCATT TACCCACACC 
AATGGAGCAA GACTAAAGCT TTCCAAGGCC AAAGACCAGT ACCGCTTGCT GTACAGGGAA 
CTGGAGCAAT TAATTCAACT AAATGCCACC ACAATGCACC ATAAAAATCT GCTGGAAAGC 
CTACAGAGTT TGCGAGCCGC ATACGGCGAT GCCCCGCTGA AGTCGGAACC TGGAGCAAGT 
CTCCTGCGCA CCTACACGGA ATCCCCACTC TCTCCCGAAC GGCTGGAGCC CATCAGTAGC 
GTCGGTGCTA GTGGCAGCAG CAGCTCCAAT AGCCTTCTCA AGGCGAGCAA GCGTCGCATG 
TCTAGTTGTG GGCAAAGATC CCTGCTAGAT ATTATATCCT CAGCAGAGCG AAGCCAGTCC 
AACAAACGAT TGGATTTCTC GGGACGCCTT TGCACTCCGT TGGGTCAAGT GGCCAAGCTG 
TATCCGGATT TTGGTACCAA GGACAAAGAC ACAGTTACGA CGGGGGCCAG TATTACGCCC 
AATGTTAAGG AGGAATCCGT ACGTAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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asun cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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