Mhcl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mhcl
Entrez Gene ID	41955
Full Name	Myosin heavy chain-like
Synonyms	CG10218,CG10224,CG31045,DmPDZ,Dmel\CG31045,MHCL(PDZ),Myo7A
General protein information	Preferred Names Myosin heavy chain-like Names CG31045 gene product from transcript CG31045-RA CG31045-PA CG31045-PB CG31045-PC CG31045-PD CG31045-PF CG31045-PG CG31045-PH CG31045-PI Mhcl-PA Mhcl-PB Mhcl-PC Mhcl-PD Mhcl-PF Mhcl-PG Mhcl-PH Mhcl-PI Myosin-heavy-chain-like PDZ-myosin myosin 18 myosin XVIII myosin XVIII (PDZ) myosin heavy chain myosin heavy chain-like
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 89B2-89B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001043251.2	NP_001036716.1	myosin heavy chain-like, isoform F
NM_169700.3	NP_732108.2	myosin heavy chain-like, isoform A
NM_001043250.2	NP_001036715.1	myosin heavy chain-like, isoform G
NM_001202304.1	NP_001189233.1	myosin heavy chain-like, isoform H
NM_169703.1	NP_732111.1	myosin heavy chain-like, isoform D
NM_170650.4	NP_732110.2	myosin heavy chain-like, isoform C
NM_169701.3	NP_732109.2	myosin heavy chain-like, isoform B
NM_001275694.1	NP_001262623.1	myosin heavy chain-like, isoform I

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Mhcl	NP_001036715.1
Danio rerio (zebrafish)	myo18ab	XP_005157670.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011138	XP_564385.3
Homo sapiens (human)	MYO18A	NP_976063.1
	MYO18A	XP_001110924.1
Bos taurus (cattle)	MYO18A	XP_003587436.1
Mus musculus (house mouse)	Myo18a	NP_035716.1
Danio rerio (zebrafish)	myo18aa	XP_695256.4
Rattus norvegicus (Norway rat)	Myo18a	NP_001165608.1
Canis lupus familiaris (dog)	MYO18A	XP_005624934.1
Gallus gallus (chicken)	MYO18A	XP_004946669.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mhcl deficient embryos develop a wild-type musculature.
Title: Myosin heavy chain-like localizes at cell contact sites during Drosophila myoblast fusion and interacts in vitro with Rolling pebbles 7.

Drosophila melanogaster myosin-18 represents a highly divergent motor with actin tethering properties
Title: Drosophila melanogaster myosin-18 represents a highly divergent motor with actin tethering properties.

The following Mhcl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mhcl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa07927	NM_001202304.1 Latest version!	Drosophila melanogaster myosin heavy chain-like, transcript variant H (Mhcl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa05086	NM_170650.4 Latest version!	Drosophila melanogaster myosin heavy chain-like, transcript variant C (Mhcl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa05083	NM_169703.1 Latest version!	Drosophila melanogaster myosin heavy chain-like, transcript variant D (Mhcl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa08422	NM_001275694.1 Latest version!	Drosophila melanogaster myosin heavy chain-like, transcript variant I (Mhcl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa05085	NM_001043251.2 Latest version!	Drosophila melanogaster myosin heavy chain-like, transcript variant F (Mhcl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa05081	NM_169700.3 Latest version!	Drosophila melanogaster myosin heavy chain-like, transcript variant A (Mhcl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa05082	NM_169701.3 Latest version!	Drosophila melanogaster myosin heavy chain-like, transcript variant B (Mhcl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa05084	NM_001043250.2 Latest version!	Drosophila melanogaster myosin heavy chain-like, transcript variant G (Mhcl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mhcl ( NM_001202304.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189233.1 Drosophila melanogaster myosin heavy chain-like, transcript variant H (Mhcl), mRNA.

Price & Availability↑

CloneID	OFa07927
Clone ID Related Accession (Same CDS sequence)	NM_001202304.1
Accession Version	NM_001202304.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain-like, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTTTCC GCTACTCAAC TCGGCTGATT CAAGACACCC GTCTGGATCT TGAATTCAAG 
GAGGAGAAAC TTGCATCGCT ACAGCGCGAG CTGGAGGAGA TGACCTTTGG TGGCGGCACC 
GAGGAGGAGT TCGCCCAACT GCGGCGCTCC AAAAATGAAA CAGAGCGTCG GGCCAAGGAA 
CAGGAGGAAG AACTGGACGA GATGGCCGGT CAGATACAGC TGCTCGAACA AGCCAAGCTT 
CGCCTGGAGA TGACCCTGGA AACAATGCGC AAAGAAGCGC GCCGTGAGTC CCAGCAGCGC 
GACGAAGAGC TGGAAGAAGT ACGCGGTAAC GGCTACAAGA AGATTAAAGC CCTTGAATGC 
CAGCTGGAAA CTGAGCACGA GGAGCGGACT CTGCTGCTGC GCGAGAAGCA CGAGCTAGAG 
CGACGTCTCT CCTCCATGGA GGATCGCGAT CGTGTTGATC GCGATGCCGA GGAAGCGCTC 
AACCAAAAAC TGCGACGCGA TCTTCGCAAA TACAAGGCCC TGCTCAAGGA CGCCCAGACA 
CAGTTGGAGC GGCTTAAGGC TGACACGCCT GGCAAGACGC TTATAAGACA ACTGCGTAAT 
CAACTGGAGG ATGCCGAATC TGCTCGTTCG CTGGCTATGA AAGCGCGACA AACTGCGGAA 
GCCGAACTTA CTGAAGTGCA GGCCATGTTC GATGAGTCGC ATCGAGCTAG GAATGACGCG 
GAGGAGCGAG CCAATGCCGC ACACAGAGAT CGGGCTGAGC TGCAGGCCCA GATCGAGGAG 
AACGAGGAGG AGCTGGGCGA GCTGATGAAG AAGTACAGCG CCACGGTAAA GCAACTAAAT 
ACAGAGCAAA TCAACGTATC CGAGGCTGAG TTTAAACTCA ATGAAATGGA AGCAGAGCGC 
AATAACCTCA AGGAGCAGGT GGCCGAGCTG CAGCACCGAC TGGACAACGT TGAGAACTTG 
GGGGATCCAT CTATGGCCAT GATGTCCAAG CGACTGGAGC TGCGCACCAA GGAATTAGAG 
TCTCGACTGG AACTAGAGCA GGCCACTCGG GCTCGCCTTG AAGTGCAGGT GAACCGTCAC 
AAAGAGGCTC TGGAAAAACT GCAGAACGAG GTGACGCAGT CAAAGATGCG TGAGATGCAG 
GCCCAGGATG TAATCAAAAA ATCGCAAAAG AGTCTGCGCG ACATGCGCGA AGAGTTCCAC 
GCTGTCTCCA GCCGCGAGCA GGAGTCGCTC ACAAGGCGCA AGGACCTGGA GAAGAAGGTG 
GAGCAAATGG AGTCGGAGGG AGCGGCGCTT AAAAACGATC TACGACTGGC TTTACAGCGA 
ATAGCTGATC TACAGCAGGC AATGGAGGAA GAGGGCGAGG AGGAGCTGAG CGAAAGTGAT 
GAAAGCCTCA GTTCGGTGGG CTCTATCAGT GATCTGGAAG ATCGACTTCG GCCAGTTCAT 
GTAAAACGCA GCTCACAGCA GTCCTTGAAC GGCAGCATCG GCGGCGGAGG CGGAAGCGTT 
GTCAGCTCCA CCCGCACCGT GGTGTTCGAA AAGGACGACA ACAGCCCGAG GTACGCTGAT 
AACGAAGTCA ACTCGAACAG CAGAAAGCAG CACAAGCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189233.1
CDS	278..1879
Translation	MSFRYSTRLIQDTRLDLEFKEEKLASLQRELEEMTFGGGTEEEFAQLRRSKNETERRAKEQEEELDEMAGQIQLLEQAKLRLEMTLETMRKEARRESQQRDEELEEVRGNGYKKIKALECQLETEHEERTLLLREKHELERRLSSMEDRDRVDRDAEEALNQKLRRDLRKYKALLKDAQTQLERLKADTPGKTLIRQLRNQLEDAESARSLAMKARQTAEAELTEVQAMFDESHRARNDAEERANAAHRDRAELQAQIEENEEELGELMKKYSATVKQLNTEQINVSEAEFKLNEMEAERNNLKEQVAELQHRLDNVENLGDPSMAMMSKRLELRTKELESRLELEQATRARLEVQVNRHKEALEKLQNEVTQSKMREMQAQDVIKKSQKSLRDMREEFHAVSSREQESLTRRKDLEKKVEQMESEGAALKNDLRLALQRIADLQQAMEEEGEEELSESDESLSSVGSISDLEDRLRPVHVKRSSQQSLNGSIGGGGGSVVSSTRTVVFEKDDNSPRYADNEVNSNSRKQHKH

Target ORF information:

RefSeq Version	NM_001202304.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain-like, transcript variant H (Mhcl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202304.1

ORF Insert Sequence:

ATGAGTTTCC GCTACTCAAC TCGGCTGATT CAAGACACCC GTCTGGATCT TGAATTCAAG 
GAGGAGAAAC TTGCATCGCT ACAGCGCGAG CTGGAGGAGA TGACCTTTGG TGGCGGCACC 
GAGGAGGAGT TCGCCCAACT GCGGCGCTCC AAAAATGAAA CAGAGCGTCG GGCCAAGGAA 
CAGGAGGAAG AACTGGACGA GATGGCCGGT CAGATACAGC TGCTCGAACA AGCCAAGCTT 
CGCCTGGAGA TGACCCTGGA AACAATGCGC AAAGAAGCGC GCCGTGAGTC CCAGCAGCGC 
GACGAAGAGC TGGAAGAAGT ACGCGGTAAC GGCTACAAGA AGATTAAAGC CCTTGAATGC 
CAGCTGGAAA CTGAGCACGA GGAGCGGACT CTGCTGCTGC GCGAGAAGCA CGAGCTAGAG 
CGACGTCTCT CCTCCATGGA GGATCGCGAT CGTGTTGATC GCGATGCCGA GGAAGCGCTC 
AACCAAAAAC TGCGACGCGA TCTTCGCAAA TACAAGGCCC TGCTCAAGGA CGCCCAGACA 
CAGTTGGAGC GGCTTAAGGC TGACACGCCT GGCAAGACGC TTATAAGACA ACTGCGTAAT 
CAACTGGAGG ATGCCGAATC TGCTCGTTCG CTGGCTATGA AAGCGCGACA AACTGCGGAA 
GCCGAACTTA CTGAAGTGCA GGCCATGTTC GATGAGTCGC ATCGAGCTAG GAATGACGCG 
GAGGAGCGAG CCAATGCCGC ACACAGAGAT CGGGCTGAGC TGCAGGCCCA GATCGAGGAG 
AACGAGGAGG AGCTGGGCGA GCTGATGAAG AAGTACAGCG CCACGGTAAA GCAACTAAAT 
ACAGAGCAAA TCAACGTATC CGAGGCTGAG TTTAAACTCA ATGAAATGGA AGCAGAGCGC 
AATAACCTCA AGGAGCAGGT GGCCGAGCTG CAGCACCGAC TGGACAACGT TGAGAACTTG 
GGGGATCCAT CTATGGCCAT GATGTCCAAG CGACTGGAGC TGCGCACCAA GGAATTAGAG 
TCTCGACTGG AACTAGAGCA GGCCACTCGG GCTCGCCTTG AAGTGCAGGT GAACCGTCAC 
AAAGAGGCTC TGGAAAAACT GCAGAACGAG GTGACGCAGT CAAAGATGCG TGAGATGCAG 
GCCCAGGATG TAATCAAAAA ATCGCAAAAG AGTCTGCGCG ACATGCGCGA AGAGTTCCAC 
GCTGTCTCCA GCCGCGAGCA GGAGTCGCTC ACAAGGCGCA AGGACCTGGA GAAGAAGGTG 
GAGCAAATGG AGTCGGAGGG AGCGGCGCTT AAAAACGATC TACGACTGGC TTTACAGCGA 
ATAGCTGATC TACAGCAGGC AATGGAGGAA GAGGGCGAGG AGGAGCTGAG CGAAAGTGAT 
GAAAGCCTCA GTTCGGTGGG CTCTATCAGT GATCTGGAAG ATCGACTTCG GCCAGTTCAT 
GTAAAACGCA GCTCACAGCA GTCCTTGAAC GGCAGCATCG GCGGCGGAGG CGGAAGCGTT 
GTCAGCTCCA CCCGCACCGT GGTGTTCGAA AAGGACGACA ACAGCCCGAG GTACGCTGAT 
AACGAAGTCA ACTCGAACAG CAGAAAGCAG CACAAGCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhcl ( NM_170650.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732110.2 Drosophila melanogaster myosin heavy chain-like, transcript variant C (Mhcl), mRNA.

Price & Availability↑

CloneID	OFa05086
Clone ID Related Accession (Same CDS sequence)	NM_170650.4
Accession Version	NM_170650.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain-like, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 31, 2011 this sequence version replaced NM_170650.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGTCGG ATAAAAGTGC AGTTTGTTTG CCGTTGAGTG GCAACGCGAG TGTCGCCGCA 
TCATTTCGCA ACAGTGCTCA AATCCTGAAC CTATTCTATG GCGAGGGGCA TGAGGATTTT 
GTGGTGCCGC AGCCAATCCT GGGACTGCTG ACCATCGGCT GTGCTATCGC CTGCGTCCGT 
ATAATCCTCT TCCGCTCAGG AGTTCTTAGT GAACTGGAAG CCAAGCGCGA TGTCCTGCTC 
TCAGATCGCA TTATACAGCT GCAAGCATTT TGCCGCGGCT ACTTGGCACG CAAGAAGATG 
TCCCAACGAA GGGTCCAGGA ACTGGCAGTG CGTTGCATTC AACGCAATGT GAAGGCATTC 
CTTGCCGTTC GCGACTGGCC GTGGTGGCGT CTCCTGGTGC GGGTCACTCC CCTGCTCAAC 
GTTCATCGCA CCGAGGAGCA GCTGAAGACG GCTAACGAGG AGCTCCTAAT GCTGCGAGCC 
AAGCTGGAGA AGATCGAGTG TGATCGCAGT GAGGTCAAGG CAGAAAACCA AAAGCTGGAA 
GCCAAGCTAT CCGAGCTGAC GGTGGACCTG GCCGAGGAGC GATCCACGGC TCATATAGCT 
ACCGAGCGGC TGGAGGCGGA AACCGCCGAA CGCTTGAAGC TGGAGAAGGA GCTGGGCGAT 
CAAACCAACA AGGTGAAGAA CCTTCAGGAG ACGACGGAGA AGCTGGAAAT GGAGCTGATA 
TGCGCCAAGT CCGATCTGAA TGGCATCTCT GAGGACGAGG ATGCAGAGAA CGAGGATGGG 
GTCGGCGGCG GCGTCTACAA GCTTAAGTAC GAGCGGGTGG CCAGGGAGCT GGAGTTCACC 
AAAAGGCGGT TACACACGCA GCATGAGCAC GATCTGGAAC AGCTGGTCGC GCTCAAGAAG 
CATTTGGAGA TGAAGCTTTC CGATGCCTAC GAAGAAGTTG TGGAACAACG TCAGGTTGTG 
GGCCAATGGA AGCGCAAGGC ACAGAAGATG ACCAACGAGA TGAACGATCT GCGCATGCTG 
CTCGAGGAGC AAAATGCGCG CAACAATTTG CTCGAGAAGA AGCAGCGCAA GTTCGACGCC 
GAGTGCCAGT CCCTGCAGGA TGCAGTGCGT CAGGAGCGAC AGGCCAAAGA GCGCTACGGT 
CGCGAAAAGG ACGTCCTGCA GGCCGAGAAG TTCACACTGG AGCAAACGCT GGCGGACACC 
CGTCTGGATC TTGAATTCAA GGAGGAGAAA CTTGCATCGC TACAGCGCGA GCTGGAGGAG 
ATGACCTTTG GTGGCGGCAC CGAGGAGGAG TTCGCCCAAC TGCGGCGCTC CAAAAATGAA 
ACAGAGCGTC GGGCCAAGGA ACAGGAGGAA GAACTGGACG AGATGGCCGG TCAGATACAG 
CTGCTCGAAC AAGCCAAGCT TCGCCTGGAG ATGACCCTGG AAACAATGCG CAAAGAAGCG 
CGCCGTGAGT CCCAGCAGCG CGACGAAGAG CTGGAAGAAG TACGCGGTAA CGGCTACAAG 
AAGATTAAAG CCCTTGAATG CCAGCTGGAA ACTGAGCACG AGGAGCGGAC TCTGCTGCTG 
CGCGAGAAGC ACGAGCTAGA GCGACGTCTC TCCTCCATGG AGGATCGCGA TCGTGTTGAT 
CGCGATGCCG AGGAAGCGCT CAACCAAAAA CTGCGACGCG ATCTTCGCAA ATACAAGGCC 
CTGCTCAAGG ACGCCCAGAC ACAGTTGGAG CGGCTTAAGG CTGACACGCC TGGCAAGACG 
CTTATAAGAC AACTGCGTAA TCAACTGGAG GATGCCGAAT CTGCTCGTTC GCTGGCTATG 
AAAGCGCGAC AAACTGCGGA AGCCGAACTT ACTGAAGTGC AGGCCATGTT CGATGAGTCG 
CATCGAGCTA GGAATGACGC GGAGGAGCGA GCCAATGCCG CACACAGAGA TCGGGCTGAG 
CTGCAGGCCC AGATCGAGGA GAACGAGGAG GAGCTGGGCG AGCTGATGAA GAAGTACAGC 
GCCACGGTAA AGCAACTAAA TACAGAGCAA ATCAACGTAT CCGAGGCTGA GTTTAAACTC 
AATGAAATGG AAGCAGAGCG CAATAACCTC AAGGAGCAGG TGGCCGAGCT GCAGCACCGA 
CTGGACAACG TTGAGAACTT GGGGGATCCA TCTATGGCCA TGATGTCCAA GCGACTGGAG 
CTGCGCACCA AGGAATTAGA GTCTCGACTG GAACTAGAGC AGGCCACTCG GGCTCGCCTT 
GAAGTGCAGG TGAACCGTCA CAAAGAGGCT CTGGAAAAAC TGCAGAACGA GGTGACGCAG 
TCAAAGATGC GTGAGATGCA GGCCCAGGAT GTAATCAAAA AATCGCAAAA GAGTCTGCGC 
GACATGCGCG AAGAGTTCCA CGCTGTCTCC AGCCGCGAGC AGGAGTCGCT CACAAGGCGC 
AAGGACCTGG AGAAGAAGGT GGAGCAAATG GAGTCGGAGG GAGCGGCGCT TAAAAACGAT 
CTACGACTGG CTTTACAGCG AATAGCTGAT CTACAGCAGG CAATGGAGGA AGAGGGCGAG 
GAGGAGCTGA GCGAAAGTGA TGAAAGCCTC AGTTCGGTGG GCTCTATCAG TGATCTGGAA 
GATCGACTTC GGCCAGTTCA TGTAAAACGC AGCTCACAGC AGTCCTTGAA CGGCAGCATC 
GGCGGCGGAG GCGGAAGCGT TGTCAGCTCC ACCCGCACCG TGGTGTTCGA AAAGGACGAC 
AACAGCCCGA GGTACGCTGA TAACGAAGTC AACTCGAACA GCAGAAAGCA GCACAAGCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732110.2
CDS	25..2787
Translation	MWSDKSAVCLPLSGNASVAASFRNSAQILNLFYGEGHEDFVVPQPILGLLTIGCAIACVRIILFRSGVLSELEAKRDVLLSDRIIQLQAFCRGYLARKKMSQRRVQELAVRCIQRNVKAFLAVRDWPWWRLLVRVTPLLNVHRTEEQLKTANEELLMLRAKLEKIECDRSEVKAENQKLEAKLSELTVDLAEERSTAHIATERLEAETAERLKLEKELGDQTNKVKNLQETTEKLEMELICAKSDLNGISEDEDAENEDGVGGGVYKLKYERVARELEFTKRRLHTQHEHDLEQLVALKKHLEMKLSDAYEEVVEQRQVVGQWKRKAQKMTNEMNDLRMLLEEQNARNNLLEKKQRKFDAECQSLQDAVRQERQAKERYGREKDVLQAEKFTLEQTLADTRLDLEFKEEKLASLQRELEEMTFGGGTEEEFAQLRRSKNETERRAKEQEEELDEMAGQIQLLEQAKLRLEMTLETMRKEARRESQQRDEELEEVRGNGYKKIKALECQLETEHEERTLLLREKHELERRLSSMEDRDRVDRDAEEALNQKLRRDLRKYKALLKDAQTQLERLKADTPGKTLIRQLRNQLEDAESARSLAMKARQTAEAELTEVQAMFDESHRARNDAEERANAAHRDRAELQAQIEENEEELGELMKKYSATVKQLNTEQINVSEAEFKLNEMEAERNNLKEQVAELQHRLDNVENLGDPSMAMMSKRLELRTKELESRLELEQATRARLEVQVNRHKEALEKLQNEVTQSKMREMQAQDVIKKSQKSLRDMREEFHAVSSREQESLTRRKDLEKKVEQMESEGAALKNDLRLALQRIADLQQAMEEEGEEELSESDESLSSVGSISDLEDRLRPVHVKRSSQQSLNGSIGGGGGSVVSSTRTVVFEKDDNSPRYADNEVNSNSRKQHKH

Target ORF information:

RefSeq Version	NM_170650.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain-like, transcript variant C (Mhcl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170650.4

ORF Insert Sequence:

ATGTGGTCGG ATAAAAGTGC AGTTTGTTTG CCGTTGAGTG GCAACGCGAG TGTCGCCGCA 
TCATTTCGCA ACAGTGCTCA AATCCTGAAC CTATTCTATG GCGAGGGGCA TGAGGATTTT 
GTGGTGCCGC AGCCAATCCT GGGACTGCTG ACCATCGGCT GTGCTATCGC CTGCGTCCGT 
ATAATCCTCT TCCGCTCAGG AGTTCTTAGT GAACTGGAAG CCAAGCGCGA TGTCCTGCTC 
TCAGATCGCA TTATACAGCT GCAAGCATTT TGCCGCGGCT ACTTGGCACG CAAGAAGATG 
TCCCAACGAA GGGTCCAGGA ACTGGCAGTG CGTTGCATTC AACGCAATGT GAAGGCATTC 
CTTGCCGTTC GCGACTGGCC GTGGTGGCGT CTCCTGGTGC GGGTCACTCC CCTGCTCAAC 
GTTCATCGCA CCGAGGAGCA GCTGAAGACG GCTAACGAGG AGCTCCTAAT GCTGCGAGCC 
AAGCTGGAGA AGATCGAGTG TGATCGCAGT GAGGTCAAGG CAGAAAACCA AAAGCTGGAA 
GCCAAGCTAT CCGAGCTGAC GGTGGACCTG GCCGAGGAGC GATCCACGGC TCATATAGCT 
ACCGAGCGGC TGGAGGCGGA AACCGCCGAA CGCTTGAAGC TGGAGAAGGA GCTGGGCGAT 
CAAACCAACA AGGTGAAGAA CCTTCAGGAG ACGACGGAGA AGCTGGAAAT GGAGCTGATA 
TGCGCCAAGT CCGATCTGAA TGGCATCTCT GAGGACGAGG ATGCAGAGAA CGAGGATGGG 
GTCGGCGGCG GCGTCTACAA GCTTAAGTAC GAGCGGGTGG CCAGGGAGCT GGAGTTCACC 
AAAAGGCGGT TACACACGCA GCATGAGCAC GATCTGGAAC AGCTGGTCGC GCTCAAGAAG 
CATTTGGAGA TGAAGCTTTC CGATGCCTAC GAAGAAGTTG TGGAACAACG TCAGGTTGTG 
GGCCAATGGA AGCGCAAGGC ACAGAAGATG ACCAACGAGA TGAACGATCT GCGCATGCTG 
CTCGAGGAGC AAAATGCGCG CAACAATTTG CTCGAGAAGA AGCAGCGCAA GTTCGACGCC 
GAGTGCCAGT CCCTGCAGGA TGCAGTGCGT CAGGAGCGAC AGGCCAAAGA GCGCTACGGT 
CGCGAAAAGG ACGTCCTGCA GGCCGAGAAG TTCACACTGG AGCAAACGCT GGCGGACACC 
CGTCTGGATC TTGAATTCAA GGAGGAGAAA CTTGCATCGC TACAGCGCGA GCTGGAGGAG 
ATGACCTTTG GTGGCGGCAC CGAGGAGGAG TTCGCCCAAC TGCGGCGCTC CAAAAATGAA 
ACAGAGCGTC GGGCCAAGGA ACAGGAGGAA GAACTGGACG AGATGGCCGG TCAGATACAG 
CTGCTCGAAC AAGCCAAGCT TCGCCTGGAG ATGACCCTGG AAACAATGCG CAAAGAAGCG 
CGCCGTGAGT CCCAGCAGCG CGACGAAGAG CTGGAAGAAG TACGCGGTAA CGGCTACAAG 
AAGATTAAAG CCCTTGAATG CCAGCTGGAA ACTGAGCACG AGGAGCGGAC TCTGCTGCTG 
CGCGAGAAGC ACGAGCTAGA GCGACGTCTC TCCTCCATGG AGGATCGCGA TCGTGTTGAT 
CGCGATGCCG AGGAAGCGCT CAACCAAAAA CTGCGACGCG ATCTTCGCAA ATACAAGGCC 
CTGCTCAAGG ACGCCCAGAC ACAGTTGGAG CGGCTTAAGG CTGACACGCC TGGCAAGACG 
CTTATAAGAC AACTGCGTAA TCAACTGGAG GATGCCGAAT CTGCTCGTTC GCTGGCTATG 
AAAGCGCGAC AAACTGCGGA AGCCGAACTT ACTGAAGTGC AGGCCATGTT CGATGAGTCG 
CATCGAGCTA GGAATGACGC GGAGGAGCGA GCCAATGCCG CACACAGAGA TCGGGCTGAG 
CTGCAGGCCC AGATCGAGGA GAACGAGGAG GAGCTGGGCG AGCTGATGAA GAAGTACAGC 
GCCACGGTAA AGCAACTAAA TACAGAGCAA ATCAACGTAT CCGAGGCTGA GTTTAAACTC 
AATGAAATGG AAGCAGAGCG CAATAACCTC AAGGAGCAGG TGGCCGAGCT GCAGCACCGA 
CTGGACAACG TTGAGAACTT GGGGGATCCA TCTATGGCCA TGATGTCCAA GCGACTGGAG 
CTGCGCACCA AGGAATTAGA GTCTCGACTG GAACTAGAGC AGGCCACTCG GGCTCGCCTT 
GAAGTGCAGG TGAACCGTCA CAAAGAGGCT CTGGAAAAAC TGCAGAACGA GGTGACGCAG 
TCAAAGATGC GTGAGATGCA GGCCCAGGAT GTAATCAAAA AATCGCAAAA GAGTCTGCGC 
GACATGCGCG AAGAGTTCCA CGCTGTCTCC AGCCGCGAGC AGGAGTCGCT CACAAGGCGC 
AAGGACCTGG AGAAGAAGGT GGAGCAAATG GAGTCGGAGG GAGCGGCGCT TAAAAACGAT 
CTACGACTGG CTTTACAGCG AATAGCTGAT CTACAGCAGG CAATGGAGGA AGAGGGCGAG 
GAGGAGCTGA GCGAAAGTGA TGAAAGCCTC AGTTCGGTGG GCTCTATCAG TGATCTGGAA 
GATCGACTTC GGCCAGTTCA TGTAAAACGC AGCTCACAGC AGTCCTTGAA CGGCAGCATC 
GGCGGCGGAG GCGGAAGCGT TGTCAGCTCC ACCCGCACCG TGGTGTTCGA AAAGGACGAC 
AACAGCCCGA GGTACGCTGA TAACGAAGTC AACTCGAACA GCAGAAAGCA GCACAAGCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhcl ( NM_169703.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732111.1 Drosophila melanogaster myosin heavy chain-like, transcript variant D (Mhcl), mRNA.

Price & Availability↑

CloneID	OFa05083
Clone ID Related Accession (Same CDS sequence)	NM_169703.1
Accession Version	NM_169703.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain-like, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCTCCCC TGTCGCCTGC TAATGAGGCT ACTGCTGAAA TTGCAGCTTC AGGTGAGGAG 
AATGCAGGAC CTGTAGAATC GGACCGATTG GAAAAGGAGA AGTTCTTGGC TCGCGTACTA 
GCAGCCATGA ACGGCGGCCC TAGATCAGGA AACCTCGCCT CCAACAACAG CAGCGGTTCA 
TCGTTGAACT CTACGTTGAG TGGATCTGCC CCTCTGGCAC GCCCACCCAA ACCACCTGCC 
AGTCAAGCCA GGAAACAGCT CCGTCTGCTG CCCAAGCAGC GGGAGCAGAG TCCTGTGCTG 
AGCAATGGCC ACACAACACG TTCCACACTG AGTCACCAGC ACACCACCAA CAAATACACA 
CTGAAATCCA CTGGTGGCAA TGCCGGCAGC TCGACGCTTT CATCGTCCTT GCTGGGTAAT 
AAGGACTCTG AAATACCGTA CGAGGTCTCG CTGAAACTGC CACTGAATCC TCCCACAGGA 
GCGGACAGTG ACCGTCATCG CCTAGTAAAC CGTTCGCCCA GTCCGGCCCT TTCCGTGTCG 
TCGCGATGCT CAACACAGTC CCAGTCATCG ATCTCCAACT TCACCTCCAA CTCAAGACTG 
CAGACGCCAC CGAGAAGGGT CTTCCCGCAG AGTTACACGC GCGGATCGGG TGGAGTCGAT 
CCCTACGAAA TGCGACAGTT GGAAAGTTCC CCTGTGGTAT TTGATGGCAA TCTCTCCTTT 
GTGCTCGGAT GCCAAAGGCA GCCGGTGTAT CAGTCGATGC GTGCTTCGCC CTCTTTTGAG 
GATCCGCGTA CATCATCCGC TTATCTGAGT GAGAAGATAC AGAATTTCCT AAAGCGCACA 
GATCATGTAC AGGAGGAGTG GACCGCTATG TCCAGGAGGA CGCGAAGAAT CAACAGCAAT 
AGTGGTCGTG CAGGAGGATC TGAAAGTCGC TGCACTACCC CAGGCAGCAC CTGTTCGGGA 
TACGATGACT ACGATACCAT CTCGCTAATA GAGCGTCAGC GAGAGAGGAA CAGCATGGAG 
CGCTGCGGAT CGGTGGGACG CACTCGCTCC TCGCAGAACA TCTTGACCAA GGCCTTTCAA 
CTGGCCAAAC AGCTTCCCCA TACGCCAACG TCGCGTGGGA ATTCGGTTGC CCGGGATCGG 
GAGTCATCTG TGGCCACCAT TAGTCTTACA ACCGGGGACA ATGACGATGA TGATGATCGC 
ACCATTCGTG AGGAGGATGA TGAGCTATCC GAGCTGACGG TGGACCTGGC CGAGGAGCGA 
TCCACGGCTC ATATAGCTAC CGAGCGGCTG GAGGCGGAAA CCGCCGAACG CTTGAAGCTG 
GAGAAGGAGC TGGGCGATCA AACCAACAAG GTGAAGAACC TTCAGGAGAC GACGGAGAAG 
CTGGAAATGG AGCTGATATG CGCCAAGTCC GATCTGAATG GCATCTCTGA GGACGAGGAT 
GCAGAGAACG AGGATGGGGT CGGCGGCGGC GTCTACAAGC TTAAGTACGA GCGGGTGGCC 
AGGGAGCTGG AGTTCACCAA AAGGCGGTTA CACACGCAGC ATGAGCACGA TCTGGAACAG 
CTGGTCGCGC TCAAGAAGCA TTTGGAGATG AAGCTTTCCG ATGCCTACGA AGAAGTTGTG 
GAACAACGTC AGGTTGTGGG CCAATGGAAG CGCAAGGCAC AGAAGATGAC CAACGAGATG 
AACGATCTGC GCATGCTGCT CGAGGAGCAA AATGCGCGCA ACAATTTGCT CGAGAAGAAG 
CAGCGCAAGT TCGACGCCGA GTGCCAGTCC CTGCAGGATG CAGTGCGTCA GGAGCGACAG 
GCCAAAGAGC GCTACGGTCG CGAAAAGGAC GTCCTGCAGG CCGAGAAGTT CACACTGGAG 
CAAACGCTGG CGGACACCCG TCTGGATCTT GAATTCAAGG AGGAGAAACT TGCATCGCTA 
CAGCGCGAGC TGGAGGAGAT GACCTTTGGT GGCGGCACCG AGGAGGAGTT CGCCCAACTG 
CGGCGCTCCA AAAATGAAAC AGAGCGTCGG GCCAAGGAAC AGGAGGAAGA ACTGGACGAG 
ATGGCCGGTC AGATACAGCT GCTCGAACAA GCCAAGCTTC GCCTGGAGAT GACCCTGGAA 
ACAATGCGCA AAGAAGCGCG CCGTGAGTCC CAGCAGCGCG ACGAAGAGCT GGAAGAAGTA 
CGCGGTAACG GCTACAAGAA GATTAAAGCC CTTGAATGCC AGCTGGAAAC TGAGCACGAG 
GAGCGGACTC TGCTGCTGCG CGAGAAGCAC GAGCTAGAGC GACGTCTCTC CTCCATGGAG 
GATCGCGATC GTGTTGATCG CGATGCCGAG GAAGCGCTCA ACCAAAAACT GCGACGCGAT 
CTTCGCAAAT ACAAGGCCCT GCTCAAGGAC GCCCAGACAC AGTTGGAGCG GCTTAAGGCT 
GACACGCCTG GCAAGACGCT TATAAGACAA CTGCGTAATC AACTGGAGGA TGCCGAATCT 
GCTCGTTCGC TGGCTATGAA AGCGCGACAA ACTGCGGAAG CCGAACTTAC TGAAGTGCAG 
GCCATGTTCG ATGAGTCGCA TCGAGCTAGG AATGACGCGG AGGAGCGAGC CAATGCCGCA 
CACAGAGATC GGGCTGAGCT GCAGGCCCAG ATCGAGGAGA ACGAGGAGGA GCTGGGCGAG 
CTGATGAAGA AGTACAGCGC CACGGTAAAG CAACTAAATA CAGAGCAAAT CAACGTATCC 
GAGGCTGAGT TTAAACTCAA TGAAATGGAA GCAGAGCGCA ATAACCTCAA GGAGCAGGTG 
GCCGAGCTGC AGCACCGACT GGACAACGTT GAGAACTTGG GGGATCCATC TATGGCCATG 
ATGTCCAAGC GACTGGAGCT GCGCACCAAG GAATTAGAGT CTCGACTGGA ACTAGAGCAG 
GCCACTCGGG CTCGCCTTGA AGTGCAGGTG AACCGTCACA AAGAGGCTCT GGAAAAACTG 
CAGAACGAGG TGACGCAGTC AAAGATGCGT GAGATGCAGG CCCAGGATGT AATCAAAAAA 
TCGCAAAAGA GTCTGCGCGA CATGCGCGAA GAGTTCCACG CTGTCTCCAG CCGCGAGCAG 
GAGTCGCTCA CAAGGCGCAA GGACCTGGAG AAGAAGGTGG AGCAAATGGA GTCGGAGGGA 
GCGGCGCTTA AAAACGATCT ACGACTGGCT TTACAGCGAA TAGCTGATCT ACAGCAGGCA 
ATGGAGGAAG AGGGCGAGGA GGAGCTGAGC GAAAGTGATG AAAGCCTCAG TTCGGTGGGC 
TCTATCAGTG ATCTGGAAGA TCGACTTCGG CCAGTTCATG TAAAACGCAG CTCACAGCAG 
TCCTTGAACG GCAGCATCGG CGGCGGAGGC GGAAGCGTTG TCAGCTCCAC CCGCACCGTG 
GTGTTCGAAA AGGACGACAA CAGCCCGAGA AATAACGCCA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732111.1
CDS	524..3928
Translation	MPPLSPANEATAEIAASGEENAGPVESDRLEKEKFLARVLAAMNGGPRSGNLASNNSSGSSLNSTLSGSAPLARPPKPPASQARKQLRLLPKQREQSPVLSNGHTTRSTLSHQHTTNKYTLKSTGGNAGSSTLSSSLLGNKDSEIPYEVSLKLPLNPPTGADSDRHRLVNRSPSPALSVSSRCSTQSQSSISNFTSNSRLQTPPRRVFPQSYTRGSGGVDPYEMRQLESSPVVFDGNLSFVLGCQRQPVYQSMRASPSFEDPRTSSAYLSEKIQNFLKRTDHVQEEWTAMSRRTRRINSNSGRAGGSESRCTTPGSTCSGYDDYDTISLIERQRERNSMERCGSVGRTRSSQNILTKAFQLAKQLPHTPTSRGNSVARDRESSVATISLTTGDNDDDDDRTIREEDDELSELTVDLAEERSTAHIATERLEAETAERLKLEKELGDQTNKVKNLQETTEKLEMELICAKSDLNGISEDEDAENEDGVGGGVYKLKYERVARELEFTKRRLHTQHEHDLEQLVALKKHLEMKLSDAYEEVVEQRQVVGQWKRKAQKMTNEMNDLRMLLEEQNARNNLLEKKQRKFDAECQSLQDAVRQERQAKERYGREKDVLQAEKFTLEQTLADTRLDLEFKEEKLASLQRELEEMTFGGGTEEEFAQLRRSKNETERRAKEQEEELDEMAGQIQLLEQAKLRLEMTLETMRKEARRESQQRDEELEEVRGNGYKKIKALECQLETEHEERTLLLREKHELERRLSSMEDRDRVDRDAEEALNQKLRRDLRKYKALLKDAQTQLERLKADTPGKTLIRQLRNQLEDAESARSLAMKARQTAEAELTEVQAMFDESHRARNDAEERANAAHRDRAELQAQIEENEEELGELMKKYSATVKQLNTEQINVSEAEFKLNEMEAERNNLKEQVAELQHRLDNVENLGDPSMAMMSKRLELRTKELESRLELEQATRARLEVQVNRHKEALEKLQNEVTQSKMREMQAQDVIKKSQKSLRDMREEFHAVSSREQESLTRRKDLEKKVEQMESEGAALKNDLRLALQRIADLQQAMEEEGEEELSESDESLSSVGSISDLEDRLRPVHVKRSSQQSLNGSIGGGGGSVVSSTRTVVFEKDDNSPRNNAK

Target ORF information:

RefSeq Version	NM_169703.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain-like, transcript variant D (Mhcl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169703.1

ORF Insert Sequence:

ATGCCTCCCC TGTCGCCTGC TAATGAGGCT ACTGCTGAAA TTGCAGCTTC AGGTGAGGAG 
AATGCAGGAC CTGTAGAATC GGACCGATTG GAAAAGGAGA AGTTCTTGGC TCGCGTACTA 
GCAGCCATGA ACGGCGGCCC TAGATCAGGA AACCTCGCCT CCAACAACAG CAGCGGTTCA 
TCGTTGAACT CTACGTTGAG TGGATCTGCC CCTCTGGCAC GCCCACCCAA ACCACCTGCC 
AGTCAAGCCA GGAAACAGCT CCGTCTGCTG CCCAAGCAGC GGGAGCAGAG TCCTGTGCTG 
AGCAATGGCC ACACAACACG TTCCACACTG AGTCACCAGC ACACCACCAA CAAATACACA 
CTGAAATCCA CTGGTGGCAA TGCCGGCAGC TCGACGCTTT CATCGTCCTT GCTGGGTAAT 
AAGGACTCTG AAATACCGTA CGAGGTCTCG CTGAAACTGC CACTGAATCC TCCCACAGGA 
GCGGACAGTG ACCGTCATCG CCTAGTAAAC CGTTCGCCCA GTCCGGCCCT TTCCGTGTCG 
TCGCGATGCT CAACACAGTC CCAGTCATCG ATCTCCAACT TCACCTCCAA CTCAAGACTG 
CAGACGCCAC CGAGAAGGGT CTTCCCGCAG AGTTACACGC GCGGATCGGG TGGAGTCGAT 
CCCTACGAAA TGCGACAGTT GGAAAGTTCC CCTGTGGTAT TTGATGGCAA TCTCTCCTTT 
GTGCTCGGAT GCCAAAGGCA GCCGGTGTAT CAGTCGATGC GTGCTTCGCC CTCTTTTGAG 
GATCCGCGTA CATCATCCGC TTATCTGAGT GAGAAGATAC AGAATTTCCT AAAGCGCACA 
GATCATGTAC AGGAGGAGTG GACCGCTATG TCCAGGAGGA CGCGAAGAAT CAACAGCAAT 
AGTGGTCGTG CAGGAGGATC TGAAAGTCGC TGCACTACCC CAGGCAGCAC CTGTTCGGGA 
TACGATGACT ACGATACCAT CTCGCTAATA GAGCGTCAGC GAGAGAGGAA CAGCATGGAG 
CGCTGCGGAT CGGTGGGACG CACTCGCTCC TCGCAGAACA TCTTGACCAA GGCCTTTCAA 
CTGGCCAAAC AGCTTCCCCA TACGCCAACG TCGCGTGGGA ATTCGGTTGC CCGGGATCGG 
GAGTCATCTG TGGCCACCAT TAGTCTTACA ACCGGGGACA ATGACGATGA TGATGATCGC 
ACCATTCGTG AGGAGGATGA TGAGCTATCC GAGCTGACGG TGGACCTGGC CGAGGAGCGA 
TCCACGGCTC ATATAGCTAC CGAGCGGCTG GAGGCGGAAA CCGCCGAACG CTTGAAGCTG 
GAGAAGGAGC TGGGCGATCA AACCAACAAG GTGAAGAACC TTCAGGAGAC GACGGAGAAG 
CTGGAAATGG AGCTGATATG CGCCAAGTCC GATCTGAATG GCATCTCTGA GGACGAGGAT 
GCAGAGAACG AGGATGGGGT CGGCGGCGGC GTCTACAAGC TTAAGTACGA GCGGGTGGCC 
AGGGAGCTGG AGTTCACCAA AAGGCGGTTA CACACGCAGC ATGAGCACGA TCTGGAACAG 
CTGGTCGCGC TCAAGAAGCA TTTGGAGATG AAGCTTTCCG ATGCCTACGA AGAAGTTGTG 
GAACAACGTC AGGTTGTGGG CCAATGGAAG CGCAAGGCAC AGAAGATGAC CAACGAGATG 
AACGATCTGC GCATGCTGCT CGAGGAGCAA AATGCGCGCA ACAATTTGCT CGAGAAGAAG 
CAGCGCAAGT TCGACGCCGA GTGCCAGTCC CTGCAGGATG CAGTGCGTCA GGAGCGACAG 
GCCAAAGAGC GCTACGGTCG CGAAAAGGAC GTCCTGCAGG CCGAGAAGTT CACACTGGAG 
CAAACGCTGG CGGACACCCG TCTGGATCTT GAATTCAAGG AGGAGAAACT TGCATCGCTA 
CAGCGCGAGC TGGAGGAGAT GACCTTTGGT GGCGGCACCG AGGAGGAGTT CGCCCAACTG 
CGGCGCTCCA AAAATGAAAC AGAGCGTCGG GCCAAGGAAC AGGAGGAAGA ACTGGACGAG 
ATGGCCGGTC AGATACAGCT GCTCGAACAA GCCAAGCTTC GCCTGGAGAT GACCCTGGAA 
ACAATGCGCA AAGAAGCGCG CCGTGAGTCC CAGCAGCGCG ACGAAGAGCT GGAAGAAGTA 
CGCGGTAACG GCTACAAGAA GATTAAAGCC CTTGAATGCC AGCTGGAAAC TGAGCACGAG 
GAGCGGACTC TGCTGCTGCG CGAGAAGCAC GAGCTAGAGC GACGTCTCTC CTCCATGGAG 
GATCGCGATC GTGTTGATCG CGATGCCGAG GAAGCGCTCA ACCAAAAACT GCGACGCGAT 
CTTCGCAAAT ACAAGGCCCT GCTCAAGGAC GCCCAGACAC AGTTGGAGCG GCTTAAGGCT 
GACACGCCTG GCAAGACGCT TATAAGACAA CTGCGTAATC AACTGGAGGA TGCCGAATCT 
GCTCGTTCGC TGGCTATGAA AGCGCGACAA ACTGCGGAAG CCGAACTTAC TGAAGTGCAG 
GCCATGTTCG ATGAGTCGCA TCGAGCTAGG AATGACGCGG AGGAGCGAGC CAATGCCGCA 
CACAGAGATC GGGCTGAGCT GCAGGCCCAG ATCGAGGAGA ACGAGGAGGA GCTGGGCGAG 
CTGATGAAGA AGTACAGCGC CACGGTAAAG CAACTAAATA CAGAGCAAAT CAACGTATCC 
GAGGCTGAGT TTAAACTCAA TGAAATGGAA GCAGAGCGCA ATAACCTCAA GGAGCAGGTG 
GCCGAGCTGC AGCACCGACT GGACAACGTT GAGAACTTGG GGGATCCATC TATGGCCATG 
ATGTCCAAGC GACTGGAGCT GCGCACCAAG GAATTAGAGT CTCGACTGGA ACTAGAGCAG 
GCCACTCGGG CTCGCCTTGA AGTGCAGGTG AACCGTCACA AAGAGGCTCT GGAAAAACTG 
CAGAACGAGG TGACGCAGTC AAAGATGCGT GAGATGCAGG CCCAGGATGT AATCAAAAAA 
TCGCAAAAGA GTCTGCGCGA CATGCGCGAA GAGTTCCACG CTGTCTCCAG CCGCGAGCAG 
GAGTCGCTCA CAAGGCGCAA GGACCTGGAG AAGAAGGTGG AGCAAATGGA GTCGGAGGGA 
GCGGCGCTTA AAAACGATCT ACGACTGGCT TTACAGCGAA TAGCTGATCT ACAGCAGGCA 
ATGGAGGAAG AGGGCGAGGA GGAGCTGAGC GAAAGTGATG AAAGCCTCAG TTCGGTGGGC 
TCTATCAGTG ATCTGGAAGA TCGACTTCGG CCAGTTCATG TAAAACGCAG CTCACAGCAG 
TCCTTGAACG GCAGCATCGG CGGCGGAGGC GGAAGCGTTG TCAGCTCCAC CCGCACCGTG 
GTGTTCGAAA AGGACGACAA CAGCCCGAGA AATAACGCCA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhcl ( NM_001275694.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262623.1 Drosophila melanogaster myosin heavy chain-like, transcript variant I (Mhcl), mRNA.

Price & Availability↑

CloneID	OFa08422
Clone ID Related Accession (Same CDS sequence)	NM_001275694.1
Accession Version	NM_001275694.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain-like, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCTCCCC TGTCGCCTGC TAATGAGGCT ACTGCTGAAA TTGCAGCTTC AGGTGAGGAG 
AATGCAGGAC CTGTAGAATC GGACCGATTG GAAAAGGAGA AGTTCTTGGC TCGCGTACTA 
GCAGCCATGA ACGGCGGCCC TAGATCAGGA AACCTCGCCT CCAACAACAG CAGCGGTTCA 
TCGTTGAACT CTACGTTGAG TGGATCTGCC CCTCTGGCAC GCCCACCCAA ACCACCTGCC 
AGTCAAGCCA GGAAACAGCT CCGTCTGCTG CCCAAGCAGC GGGAGCAGAG TCCTGTGCTG 
AGCAATGGCC ACACAACACG TTCCACACTG AGTCACCAGC ACACCACCAA CAAATACACA 
CTGAAATCCA CTGGTGGCAA TGCCGGCAGC TCGACGCTTT CATCGTCCTT GCTGGGAGCG 
GACAGTGACC GTCATCGCCT AGTAAACCGT TCGCCCAGTC CGGCCCTTTC CGTGTCGTCG 
CGATGCTCAA CACAGTCCCA GTCATCGATC TCCAACTTCA CCTCCAACTC AAGACTGCAG 
ACGCCACCGA GAAGGGTCTT CCCGCAGAGT TACACGCGCG GATCGGGTGG AGTCGATCCC 
TACGAAATGC GACAGTTGGA AAGTTCCCCT GTGGTATTTG ATGGCAATCT CTCCTTTGTG 
CTCGGATGCC AAAGGCAGCC GGTGTATCAG TCGATGCGTG CTTCGCCCTC TTTTGAGGAT 
CCGCGTACAT CATCCGCTTA TCTGAGTGAG AAGATACAGA ATTTCCTAAA GCGCACAGAT 
CATGTACAGG AGGAGTGGAC CGCTATGTCC AGGAGGACGC GAAGAATCAA CAGCAATAGT 
GGTCGTGCAG GAGGATCTGA AAGTCGCTGC ACTACCCCAG GCAGCACCTG TTCGGGATAC 
GATGACTACG ATACCATCTC GCTAATAGAG CGTCAGCGAG AGAGGAACAG CATGGAGCGC 
TGCGGATCGG TGGGACGCAC TCGCTCCTCG CAGAACATCT TGACCAAGGC CTTTCAACTG 
GCCAAACAGC TTCCCCATAC GCCAACGTCG CGTGGGAATT CGGTTGCCCG GGATCGGGAG 
TCATCTGTGG CCACCATTAG TCTTACAACC GGGGACAATG ACGATGATGA TGATCGCACC 
ATTCGTGAGG AGGATGATGA GCTATCCGAG CTGACGGTGG ACCTGGCCGA GGAGCGATCC 
ACGGCTCATA TAGCTACCGA GCGGCTGGAG GCGGAAACCG CCGAACGCTT GAAGCTGGAG 
AAGGAGCTGG GCGATCAAAC CAACAAGGTG AAGAACCTTC AGGAGACGAC GGAGAAGCTG 
GAAATGGAGC TGATATGCGC CAAGTCCGAT CTGAATGGCA TCTCTGAGGA CGAGGATGCA 
GAGAACGAGG ATGGGGTCGG CGGCGGCGTC TACAAGCTTA AGTACGAGCG GGTGGCCAGG 
GAGCTGGAGT TCACCAAAAG GCGGTTACAC ACGCAGCATG AGCACGATCT GGAACAGCTG 
GTCGCGCTCA AGAAGCATTT GGAGATGAAG CTTTCCGATG CCTACGAAGA AGTTGTGGAA 
CAACGTCAGG TTGTGGGCCA ATGGAAGCGC AAGGCACAGA AGATGACCAA CGAGATGAAC 
GATCTGCGCA TGCTGCTCGA GGAGCAAAAT GCGCGCAACA ATTTGCTCGA GAAGAAGCAG 
CGCAAGTTCG ACGCCGAGTG CCAGTCCCTG CAGGATGCAG TGCGTCAGGA GCGACAGGCC 
AAAGAGCGCT ACGGTCGCGA AAAGGACGTC CTGCAGGCCG AGAAGTTCAC ACTGGAGCAA 
ACGCTGGCGG ACACCCGTCT GGATCTTGAA TTCAAGGAGG AGAAACTTGC ATCGCTACAG 
CGCGAGCTGG AGGAGATGAC CTTTGGTGGC GGCACCGAGG AGGAGTTCGC CCAACTGCGG 
CGCTCCAAAA ATGAAACAGA GCGTCGGGCC AAGGAACAGG AGGAAGAACT GGACGAGATG 
GCCGGTCAGA TACAGCTGCT CGAACAAGCC AAGCTTCGCC TGGAGATGAC CCTGGAAACA 
ATGCGCAAAG AAGCGCGCCG TGAGTCCCAG CAGCGCGACG AAGAGCTGGA AGAAGTACGC 
GGTAACGGCT ACAAGAAGAT TAAAGCCCTT GAATGCCAGC TGGAAACTGA GCACGAGGAG 
CGGACTCTGC TGCTGCGCGA GAAGCACGAG CTAGAGCGAC GTCTCTCCTC CATGGAGGAT 
CGCGATCGTG TTGATCGCGA TGCCGAGGAA GCGCTCAACC AAAAACTGCG ACGCGATCTT 
CGCAAATACA AGGCCCTGCT CAAGGACGCC CAGACACAGT TGGAGCGGCT TAAGGCTGAC 
ACGCCTGGCA AGACGCTTAT AAGACAACTG CGTAATCAAC TGGAGGATGC CGAATCTGCT 
CGTTCGCTGG CTATGAAAGC GCGACAAACT GCGGAAGCCG AACTTACTGA AGTGCAGGCC 
ATGTTCGATG AGTCGCATCG AGCTAGGAAT GACGCGGAGG AGCGAGCCAA TGCCGCACAC 
AGAGATCGGG CTGAGCTGCA GGCCCAGATC GAGGAGAACG AGGAGGAGCT GGGCGAGCTG 
ATGAAGAAGT ACAGCGCCAC GGTAAAGCAA CTAAATACAG AGCAAATCAA CGTATCCGAG 
GCTGAGTTTA AACTCAATGA AATGGAAGCA GAGCGCAATA ACCTCAAGGA GCAGGTGGCC 
GAGCTGCAGC ACCGACTGGA CAACGTTGAG AACTTGGGGG ATCCATCTAT GGCCATGATG 
TCCAAGCGAC TGGAGCTGCG CACCAAGGAA TTAGAGTCTC GACTGGAACT AGAGCAGGCC 
ACTCGGGCTC GCCTTGAAGT GCAGGTGAAC CGTCACAAAG AGGCTCTGGA AAAACTGCAG 
AACGAGGTGA CGCAGTCAAA GATGCGTGAG ATGCAGGCCC AGGATGTAAT CAAAAAATCG 
CAAAAGAGTC TGCGCGACAT GCGCGAAGAG TTCCACGCTG TCTCCAGCCG CGAGCAGGAG 
TCGCTCACAA GGCGCAAGGA CCTGGAGAAG AAGGTGGAGC AAATGGAGTC GGAGGGAGCG 
GCGCTTAAAA ACGATCTACG ACTGGCTTTA CAGCGAATAG CTGATCTACA GCAGGCAATG 
GAGGAAGAGG GCGAGGAGGA GCTGAGCGAA AGTGATGAAA GCCTCAGTTC GGTGGGCTCT 
ATCAGTGATC TGGAAGATCG ACTTCGGCCA GTTCATGTAA AACGCAGCTC ACAGCAGTCC 
TTGAACGGCA GCATCGGCGG CGGAGGCGGA AGCGTTGTCA GCTCCACCCG CACCGTGGTG 
TTCGAAAAGG ACGACAACAG CCCGAGAAAT AACGCCAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262623.1
CDS	524..3865
Translation	MPPLSPANEATAEIAASGEENAGPVESDRLEKEKFLARVLAAMNGGPRSGNLASNNSSGSSLNSTLSGSAPLARPPKPPASQARKQLRLLPKQREQSPVLSNGHTTRSTLSHQHTTNKYTLKSTGGNAGSSTLSSSLLGADSDRHRLVNRSPSPALSVSSRCSTQSQSSISNFTSNSRLQTPPRRVFPQSYTRGSGGVDPYEMRQLESSPVVFDGNLSFVLGCQRQPVYQSMRASPSFEDPRTSSAYLSEKIQNFLKRTDHVQEEWTAMSRRTRRINSNSGRAGGSESRCTTPGSTCSGYDDYDTISLIERQRERNSMERCGSVGRTRSSQNILTKAFQLAKQLPHTPTSRGNSVARDRESSVATISLTTGDNDDDDDRTIREEDDELSELTVDLAEERSTAHIATERLEAETAERLKLEKELGDQTNKVKNLQETTEKLEMELICAKSDLNGISEDEDAENEDGVGGGVYKLKYERVARELEFTKRRLHTQHEHDLEQLVALKKHLEMKLSDAYEEVVEQRQVVGQWKRKAQKMTNEMNDLRMLLEEQNARNNLLEKKQRKFDAECQSLQDAVRQERQAKERYGREKDVLQAEKFTLEQTLADTRLDLEFKEEKLASLQRELEEMTFGGGTEEEFAQLRRSKNETERRAKEQEEELDEMAGQIQLLEQAKLRLEMTLETMRKEARRESQQRDEELEEVRGNGYKKIKALECQLETEHEERTLLLREKHELERRLSSMEDRDRVDRDAEEALNQKLRRDLRKYKALLKDAQTQLERLKADTPGKTLIRQLRNQLEDAESARSLAMKARQTAEAELTEVQAMFDESHRARNDAEERANAAHRDRAELQAQIEENEEELGELMKKYSATVKQLNTEQINVSEAEFKLNEMEAERNNLKEQVAELQHRLDNVENLGDPSMAMMSKRLELRTKELESRLELEQATRARLEVQVNRHKEALEKLQNEVTQSKMREMQAQDVIKKSQKSLRDMREEFHAVSSREQESLTRRKDLEKKVEQMESEGAALKNDLRLALQRIADLQQAMEEEGEEELSESDESLSSVGSISDLEDRLRPVHVKRSSQQSLNGSIGGGGGSVVSSTRTVVFEKDDNSPRNNAK

Target ORF information:

RefSeq Version	NM_001275694.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain-like, transcript variant I (Mhcl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275694.1

ORF Insert Sequence:

ATGCCTCCCC TGTCGCCTGC TAATGAGGCT ACTGCTGAAA TTGCAGCTTC AGGTGAGGAG 
AATGCAGGAC CTGTAGAATC GGACCGATTG GAAAAGGAGA AGTTCTTGGC TCGCGTACTA 
GCAGCCATGA ACGGCGGCCC TAGATCAGGA AACCTCGCCT CCAACAACAG CAGCGGTTCA 
TCGTTGAACT CTACGTTGAG TGGATCTGCC CCTCTGGCAC GCCCACCCAA ACCACCTGCC 
AGTCAAGCCA GGAAACAGCT CCGTCTGCTG CCCAAGCAGC GGGAGCAGAG TCCTGTGCTG 
AGCAATGGCC ACACAACACG TTCCACACTG AGTCACCAGC ACACCACCAA CAAATACACA 
CTGAAATCCA CTGGTGGCAA TGCCGGCAGC TCGACGCTTT CATCGTCCTT GCTGGGAGCG 
GACAGTGACC GTCATCGCCT AGTAAACCGT TCGCCCAGTC CGGCCCTTTC CGTGTCGTCG 
CGATGCTCAA CACAGTCCCA GTCATCGATC TCCAACTTCA CCTCCAACTC AAGACTGCAG 
ACGCCACCGA GAAGGGTCTT CCCGCAGAGT TACACGCGCG GATCGGGTGG AGTCGATCCC 
TACGAAATGC GACAGTTGGA AAGTTCCCCT GTGGTATTTG ATGGCAATCT CTCCTTTGTG 
CTCGGATGCC AAAGGCAGCC GGTGTATCAG TCGATGCGTG CTTCGCCCTC TTTTGAGGAT 
CCGCGTACAT CATCCGCTTA TCTGAGTGAG AAGATACAGA ATTTCCTAAA GCGCACAGAT 
CATGTACAGG AGGAGTGGAC CGCTATGTCC AGGAGGACGC GAAGAATCAA CAGCAATAGT 
GGTCGTGCAG GAGGATCTGA AAGTCGCTGC ACTACCCCAG GCAGCACCTG TTCGGGATAC 
GATGACTACG ATACCATCTC GCTAATAGAG CGTCAGCGAG AGAGGAACAG CATGGAGCGC 
TGCGGATCGG TGGGACGCAC TCGCTCCTCG CAGAACATCT TGACCAAGGC CTTTCAACTG 
GCCAAACAGC TTCCCCATAC GCCAACGTCG CGTGGGAATT CGGTTGCCCG GGATCGGGAG 
TCATCTGTGG CCACCATTAG TCTTACAACC GGGGACAATG ACGATGATGA TGATCGCACC 
ATTCGTGAGG AGGATGATGA GCTATCCGAG CTGACGGTGG ACCTGGCCGA GGAGCGATCC 
ACGGCTCATA TAGCTACCGA GCGGCTGGAG GCGGAAACCG CCGAACGCTT GAAGCTGGAG 
AAGGAGCTGG GCGATCAAAC CAACAAGGTG AAGAACCTTC AGGAGACGAC GGAGAAGCTG 
GAAATGGAGC TGATATGCGC CAAGTCCGAT CTGAATGGCA TCTCTGAGGA CGAGGATGCA 
GAGAACGAGG ATGGGGTCGG CGGCGGCGTC TACAAGCTTA AGTACGAGCG GGTGGCCAGG 
GAGCTGGAGT TCACCAAAAG GCGGTTACAC ACGCAGCATG AGCACGATCT GGAACAGCTG 
GTCGCGCTCA AGAAGCATTT GGAGATGAAG CTTTCCGATG CCTACGAAGA AGTTGTGGAA 
CAACGTCAGG TTGTGGGCCA ATGGAAGCGC AAGGCACAGA AGATGACCAA CGAGATGAAC 
GATCTGCGCA TGCTGCTCGA GGAGCAAAAT GCGCGCAACA ATTTGCTCGA GAAGAAGCAG 
CGCAAGTTCG ACGCCGAGTG CCAGTCCCTG CAGGATGCAG TGCGTCAGGA GCGACAGGCC 
AAAGAGCGCT ACGGTCGCGA AAAGGACGTC CTGCAGGCCG AGAAGTTCAC ACTGGAGCAA 
ACGCTGGCGG ACACCCGTCT GGATCTTGAA TTCAAGGAGG AGAAACTTGC ATCGCTACAG 
CGCGAGCTGG AGGAGATGAC CTTTGGTGGC GGCACCGAGG AGGAGTTCGC CCAACTGCGG 
CGCTCCAAAA ATGAAACAGA GCGTCGGGCC AAGGAACAGG AGGAAGAACT GGACGAGATG 
GCCGGTCAGA TACAGCTGCT CGAACAAGCC AAGCTTCGCC TGGAGATGAC CCTGGAAACA 
ATGCGCAAAG AAGCGCGCCG TGAGTCCCAG CAGCGCGACG AAGAGCTGGA AGAAGTACGC 
GGTAACGGCT ACAAGAAGAT TAAAGCCCTT GAATGCCAGC TGGAAACTGA GCACGAGGAG 
CGGACTCTGC TGCTGCGCGA GAAGCACGAG CTAGAGCGAC GTCTCTCCTC CATGGAGGAT 
CGCGATCGTG TTGATCGCGA TGCCGAGGAA GCGCTCAACC AAAAACTGCG ACGCGATCTT 
CGCAAATACA AGGCCCTGCT CAAGGACGCC CAGACACAGT TGGAGCGGCT TAAGGCTGAC 
ACGCCTGGCA AGACGCTTAT AAGACAACTG CGTAATCAAC TGGAGGATGC CGAATCTGCT 
CGTTCGCTGG CTATGAAAGC GCGACAAACT GCGGAAGCCG AACTTACTGA AGTGCAGGCC 
ATGTTCGATG AGTCGCATCG AGCTAGGAAT GACGCGGAGG AGCGAGCCAA TGCCGCACAC 
AGAGATCGGG CTGAGCTGCA GGCCCAGATC GAGGAGAACG AGGAGGAGCT GGGCGAGCTG 
ATGAAGAAGT ACAGCGCCAC GGTAAAGCAA CTAAATACAG AGCAAATCAA CGTATCCGAG 
GCTGAGTTTA AACTCAATGA AATGGAAGCA GAGCGCAATA ACCTCAAGGA GCAGGTGGCC 
GAGCTGCAGC ACCGACTGGA CAACGTTGAG AACTTGGGGG ATCCATCTAT GGCCATGATG 
TCCAAGCGAC TGGAGCTGCG CACCAAGGAA TTAGAGTCTC GACTGGAACT AGAGCAGGCC 
ACTCGGGCTC GCCTTGAAGT GCAGGTGAAC CGTCACAAAG AGGCTCTGGA AAAACTGCAG 
AACGAGGTGA CGCAGTCAAA GATGCGTGAG ATGCAGGCCC AGGATGTAAT CAAAAAATCG 
CAAAAGAGTC TGCGCGACAT GCGCGAAGAG TTCCACGCTG TCTCCAGCCG CGAGCAGGAG 
TCGCTCACAA GGCGCAAGGA CCTGGAGAAG AAGGTGGAGC AAATGGAGTC GGAGGGAGCG 
GCGCTTAAAA ACGATCTACG ACTGGCTTTA CAGCGAATAG CTGATCTACA GCAGGCAATG 
GAGGAAGAGG GCGAGGAGGA GCTGAGCGAA AGTGATGAAA GCCTCAGTTC GGTGGGCTCT 
ATCAGTGATC TGGAAGATCG ACTTCGGCCA GTTCATGTAA AACGCAGCTC ACAGCAGTCC 
TTGAACGGCA GCATCGGCGG CGGAGGCGGA AGCGTTGTCA GCTCCACCCG CACCGTGGTG 
TTCGAAAAGG ACGACAACAG CCCGAGAAAT AACGCCAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhcl ( NM_001043251.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036716.1 Drosophila melanogaster myosin heavy chain-like, transcript variant F (Mhcl), mRNA.

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CloneID	OFa05085
Clone ID Related Accession (Same CDS sequence)	NM_001043251.2
Accession Version	NM_001043251.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain-like, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 31, 2011 this sequence version replaced NM_001043251.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCCTCA AGCCGAAAAG CTTTGAACAG TCGCTGCGCG GCGGTATCGT TCCGGCTGTG 
CCAGCCGCTC CAGGATCGGG CCTCAATCCA AATTTCCATA AGGGTTGTAC GGGCAAGGCG 
CATGCCTGCG GCACAAACAA ATCCCATCGA GCCGGAGGAT CAGCGACTTC AGCGTCTGGA 
GGATCCAGGG CTGGCAGACA GACGCCTAAG GCTCAGAGAA AGAATAGCAT CAGTTCGGAG 
ACGATTAGCG AAAGTGGTTC GGAGAACTCC AACAGCTGGC CATCCTCCTC ATCGGCAGCC 
GTGCCAGCTA CCGTTCCGCC ACCGAAACCT CCGCGTCTGG CGGCCACTAA AGCTAAGGAA 
GCCGCTGCCA TCGACTGCTC TCCCGTACAG CCTCCTTTTA CCCCAACCTT CGAGCGCTCC 
ACGAAACGCT CGCTCAGCTT CAGGAGCAAC AACAGCAAGA CAACGGAGGC GAAGCTTACT 
CCGGGAGCCA GTTCCTCTAT CCGTCCTCCG TGGAATATTT CCGCCGCCGC TGCATCAAAG 
TACGCCGCTG CAGCCGCCAG TGCTCCACAG TCGCCGAATC CTAGCACGAG GAGCTCTTTC 
CGACGACGCC GTGATGCCCA GAACGCTGGT CTCCATTGGC CGGCCATTTT GGCCAGTTCC 
CTGGCCGCCA GCCATAAAAA CTACGATGCA ATTTTAAAGC AAAAGAGCCG CCATGAGAAT 
GGCAATGGCG GAGGAGAAGA GGGAGGAGCC AAAGGAGTGG GCCACGATCT GGAGGCGGAT 
GTTGCGGATG CCAGTCAGCC GGAGCGCGTA TGGCTGGTGC ACCGCGGTGG TTTCACTGCC 
GCCATCCGTC TGCCGACTGC GTCTTCTGGC CGGGATGAGG AGAACAAGCT AAGCCTGCGC 
CTCCTGCATA ATGGGGAGCA GCTGACCGTC GACGAGGACG ATGTCGAGAA GCAGAACAGC 
CCCGCCCTGG ACCTTGTCGA GGACATCTGC GAGCTGAAGT ATCTGAACGA GGCGTCCGTG 
CTCCATTGCC TGCGTCAGCG ATACGCCAGC AATCTCATCC ACACCAAGGC GGGGCCCACG 
CTGCTCGTGG TCAATCCCAT GGCACCGCTG TCCCTGTACT CCGAGAAGGT TGTCTCCATG 
TTCCGCGGCT GCAAGACGGA GGATATGCCG CCCCACGTCT ATTCACTGGC CCAGACCGCC 
TACAGGAGCC TGGTCGAGAC GCGGCGCGAC CAGAGCTTGA TATTCATGGG CCGATCTGGT 
TCTGGCAAAT CCACTAGCTT CAAGCATGCA CTCAACTACC TGGCGTTGGC AGCTGGTGCC 
TATAATAATT TTATAAACGC GGAGAAGGTC AATGCCCTGT GCACCATTCT GGAGGCTTTT 
GGAAATACGA AAACTTGCCT AAACTCGAAT GCCACGCGGA TGACGCAGCT GTTAAGCCTG 
GATTTCGATC AAACCGGCCA GATTGCATCT GCCTCATTGC AAGTTCTCCT CCCAGAAAGA 
CAACGAGCTG GCCGACGACT TGGTCATGAA CATAGCTTCC ACATCATGAC GAGACTTTTG 
GCTGGCGCTG CTGGCTTGCT ACAGAAGGAG CTGCACCTGG AGAACATAAC CTCTGAGGAC 
AGCCATCCTT TTATATCGTT ATCCCAAAAG CTGGAAGATC GGCATCGAGC TGCCAATGAC 
TTTATGCGCA CTGTTCAGGC CTTTGAGACG CTGAACATTG ATGCGAAGGC GGTACGTGGG 
ATCTGGAGCA TTTTGGCGGC CATTTACCAT CTGGGCATTG CTGGCGTCAC AAAACTGGGC 
ACTGGCTCCA CAGCCCGAAC TCAGTTTGCC AATCCCACGG CCGCCCGAAA GGCCTCGGGC 
TTGTTGGGCG TGAATCTGGA GGATCTGTCA TCAGCTGCCT TTGGTCTTAC CCAACCAAAC 
GCCCCCAATG GCGGTCTGAG TCCATCGAAA TCGCCCACCT CGGACACTGG ACACGAATGG 
GCCTGGGAAT GTCTCGAGGC GTTGGTCATT GGTCTATATT CGGAGGCCTT GGCTGCAGTG 
GTGGCATTGA TCAATCGTCA GATCTGCACA TCGTCCCACA CCATAGCCTC AATTATGCTA 
ATTGACACGC CCGGCTTCCA AAATCCAGCC AGCTGTGGCC AGCAAGTGGG TGCTACTCTA 
GCGGATCTGC GTCACAACTA TCTACAGGAG CGTCTACAGA TGCTCTTCCA TCACACCACA 
CTGGTGGCTC CCCGCGATCG CTACGCCCAG GAGCTGGTAG AAATCGAGAT GGACCTGGCA 
TCGGAGTGTC ATCCGGGACC GTTGATCTCG CTTATCGACA AGGCACCTCA GAATCATGTA 
GTGCGCTCGT CCCAGCGAGA TTTGCGAGAG CATGATCGTC GAGGAATGTT GTGGCTACTG 
GATGAGGAAG CTATCTACCC CAACTCCAAC GATGACACAT TCCTTGAACG TTTGTTCTCC 
CACTACGGTG ACCGGGAACA TCATAGCCTG CTGCGCAAAT GTGCAGGTCC GCGACAATTT 
GTACTCCACC ATCTGCAAGG CACCAATCCC GTTCTCTACG CTGTCGATGG ATGGGTGCGT 
CATAGCCGGG AGCACCCAGG TATTCGGAAT GCTGTATCCC TACTACAGGA CAGCAGTCGG 
GAGGAGATCA ATCGCCTATA TATCGGCTCA CTGACTCGAG GATCGGGAGC AATGGTGTTT 
TGTGGCTCTT TTGCTGGACT GGAAGGCACT CAGTCACTTC GTCGCGTGTC CAGTATTCGC 
CGCTCCTTCA CAACGGCCGG AGTAAAGCGA AACTCCATAA TGCTGCAGGT GAAGTTCACA 
GTGGATGGAA TTATCGACAC TTTGCGCCGC ACTGGAACTC ACTTTGTGCA CTGCTATCTG 
CTGCAACATA ACGCAGGCAA ACATACCAAG TACACCGCCA ATGGATCGCC CAGCTCAGCT 
GCCGGTCAGG TGTCCAGCGA GGAGGAAATG GTCAACGTGC CACTGTTAAG GAGTCAGCTA 
CGTGGTTCAC AAGTCCTAGA AGCGGCTCGC TTGCATCGCC TTGGTTTTCC CGAATCAGTG 
CCATTGTTGG AATTTGTCAG GCGTTTCGGG CTTTTGGCCG GGGATTTGGC TAGCAATAAG 
GATGTGAGCG TGGAGCAGAT ATTAGCTGTC AATGAACTGG ATGTGGCCAG CTATCGCATA 
GGACCCAGCC AGATCCTCTT CCGCTCAGGA GTTCTTAGTG AACTGGAAGC CAAGCGCGAT 
GTCCTGCTCT CAGATCGCAT TATACAGCTG CAAGCATTTT GCCGCGGCTA CTTGGCACGC 
AAGAAGATGT CCCAACGAAG GGTCCAGGAA CTGGCAGTGC GTTGCATTCA ACGCAATGTG 
AAGGCATTCC TTGCCGTTCG CGACTGGCCG TGGTGGCGTC TCCTGGTGCG GGTCACTCCC 
CTGCTCAACG TTCATCGCAC CGAGGAGCAG CTGAAGACGG CTAACGAGGA GCTCCTAATG 
CTGCGAGCCA AGCTGGAGAA GATCGAGTGT GATCGCAGTG AGGTCAAGGC AGAAAACCAA 
AAGCTGGAAG CCAAGCTATC CGAGCTGACG GTGGACCTGG CCGAGGAGCG ATCCACGGCT 
CATATAGCTA CCGAGCGGCT GGAGGCGGAA ACCGCCGAAC GCTTGAAGCT GGAGAAGGAG 
CTGGGCGATC AAACCAACAA GGTGAAGAAC CTTCAGGAGA CGACGGAGAA GCTGGAAATG 
GAGCTGATAT GCGCCAAGTC CGATCTGAAT GGCATCTCTG AGGACGAGGA TGCAGAGAAC 
GAGGATGGGG TCGGCGGCGG CGTCTACAAG CTTAAGTACG AGCGGGTGGC CAGGGAGCTG 
GAGTTCACCA AAAGGCGGTT ACACACGCAG CATGAGCACG ATCTGGAACA GCTGGTCGCG 
CTCAAGAAGC ATTTGGAGAT GAAGCTTTCC GATGCCTACG AAGAAGTTGT GGAACAACGT 
CAGGTTGTGG GCCAATGGAA GCGCAAGGCA CAGAAGATGA CCAACGAGAT GAACGATCTG 
CGCATGCTGC TCGAGGAGCA AAATGCGCGC AACAATTTGC TCGAGAAGAA GCAGCGCAAG 
TTCGACGCCG AGTGCCAGTC CCTGCAGGAT GCAGTGCGTC AGGAGCGACA GGCCAAAGAG 
CGCTACGGTC GCGAAAAGGA CGTCCTGCAG GCCGAGAAGT TCACACTGGA GCAAACGCTG 
GCGGACACCC GTCTGGATCT TGAATTCAAG GAGGAGAAAC TTGCATCGCT ACAGCGCGAG 
CTGGAGGAGA TGACCTTTGG TGGCGGCACC GAGGAGGAGT TCGCCCAACT GCGGCGCTCC 
AAAAATGAAA CAGAGCGTCG GGCCAAGGAA CAGGAGGAAG AACTGGACGA GATGGCCGGT 
CAGATACAGC TGCTCGAACA AGCCAAGCTT CGCCTGGAGA TGACCCTGGA AACAATGCGC 
AAAGAAGCGC GCCGTGAGTC CCAGCAGCGC GACGAAGAGC TGGAAGAAGT ACGCGGTAAC 
GGCTACAAGA AGATTAAAGC CCTTGAATGC CAGCTGGAAA CTGAGCACGA GGAGCGGACT 
CTGCTGCTGC GCGAGAAGCA CGAGCTAGAG CGACGTCTCT CCTCCATGGA GGATCGCGAT 
CGTGTTGATC GCGATGCCGA GGAAGCGCTC AACCAAAAAC TGCGACGCGA TCTTCGCAAA 
TACAAGGCCC TGCTCAAGGA CGCCCAGACA CAGTTGGAGC GGCTTAAGGC TGACACGCCT 
GGCAAGACGC TTATAAGACA ACTGCGTAAT CAACTGGAGG ATGCCGAATC TGCTCGTTCG 
CTGGCTATGA AAGCGCGACA AACTGCGGAA GCCGAACTTA CTGAAGTGCA GGCCATGTTC 
GATGAGTCGC ATCGAGCTAG GAATGACGCG GAGGAGCGAG CCAATGCCGC ACACAGAGAT 
CGGGCTGAGC TGCAGGCCCA GATCGAGGAG AACGAGGAGG AGCTGGGCGA GCTGATGAAG 
AAGTACAGCG CCACGGTAAA GCAACTAAAT ACAGAGCAAA TCAACGTATC CGAGGCTGAG 
TTTAAACTCA ATGAAATGGA AGCAGAGCGC AATAACCTCA AGGAGCAGGT GGCCGAGCTG 
CAGCACCGAC TGGACAACGT TGAGAACTTG GGGGATCCAT CTATGGCCAT GATGTCCAAG 
CGACTGGAGC TGCGCACCAA GGAATTAGAG TCTCGACTGG AACTAGAGCA GGCCACTCGG 
GCTCGCCTTG AAGTGCAGGT GAACCGTCAC AAAGAGGCTC TGGAAAAACT GCAGAACGAG 
GTGACGCAGT CAAAGATGCG TGAGATGCAG GCCCAGGATG TAATCAAAAA ATCGCAAAAG 
AGTCTGCGCG ACATGCGCGA AGAGTTCCAC GCTGTCTCCA GCCGCGAGCA GGAGTCGCTC 
ACAAGGCGCA AGGACCTGGA GAAGAAGGTG GAGCAAATGG AGTCGGAGGG AGCGGCGCTT 
AAAAACGATC TACGACTGGC TTTACAGCGA ATAGCTGATC TACAGCAGGC AATGGAGGAA 
GAGGGCGAGG AGGAGCTGAG CGAAAGTGAT GAAAGCCTCA GTTCGGTGGG CTCTATCAGT 
GATCTGGAAG ATCGACTTCG GCCAGTTCAT GTAAAACGCA GCTCACAGCA GTCCTTGAAC 
GGCAGCATCG GCGGCGGAGG CGGAAGCGTT GTCAGCTCCA CCCGCACCGT GGTGTTCGAA 
AAGGACGACA ACAGCCCGAG GTACGCTGAT AACGAAGTCA ACTCGAACAG CAGAAAGCAG 
CACAAGCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036716.1
CDS	602..6373
Translation	MFLKPKSFEQSLRGGIVPAVPAAPGSGLNPNFHKGCTGKAHACGTNKSHRAGGSATSASGGSRAGRQTPKAQRKNSISSETISESGSENSNSWPSSSSAAVPATVPPPKPPRLAATKAKEAAAIDCSPVQPPFTPTFERSTKRSLSFRSNNSKTTEAKLTPGASSSIRPPWNISAAAASKYAAAAASAPQSPNPSTRSSFRRRRDAQNAGLHWPAILASSLAASHKNYDAILKQKSRHENGNGGGEEGGAKGVGHDLEADVADASQPERVWLVHRGGFTAAIRLPTASSGRDEENKLSLRLLHNGEQLTVDEDDVEKQNSPALDLVEDICELKYLNEASVLHCLRQRYASNLIHTKAGPTLLVVNPMAPLSLYSEKVVSMFRGCKTEDMPPHVYSLAQTAYRSLVETRRDQSLIFMGRSGSGKSTSFKHALNYLALAAGAYNNFINAEKVNALCTILEAFGNTKTCLNSNATRMTQLLSLDFDQTGQIASASLQVLLPERQRAGRRLGHEHSFHIMTRLLAGAAGLLQKELHLENITSEDSHPFISLSQKLEDRHRAANDFMRTVQAFETLNIDAKAVRGIWSILAAIYHLGIAGVTKLGTGSTARTQFANPTAARKASGLLGVNLEDLSSAAFGLTQPNAPNGGLSPSKSPTSDTGHEWAWECLEALVIGLYSEALAAVVALINRQICTSSHTIASIMLIDTPGFQNPASCGQQVGATLADLRHNYLQERLQMLFHHTTLVAPRDRYAQELVEIEMDLASECHPGPLISLIDKAPQNHVVRSSQRDLREHDRRGMLWLLDEEAIYPNSNDDTFLERLFSHYGDREHHSLLRKCAGPRQFVLHHLQGTNPVLYAVDGWVRHSREHPGIRNAVSLLQDSSREEINRLYIGSLTRGSGAMVFCGSFAGLEGTQSLRRVSSIRRSFTTAGVKRNSIMLQVKFTVDGIIDTLRRTGTHFVHCYLLQHNAGKHTKYTANGSPSSAAGQVSSEEEMVNVPLLRSQLRGSQVLEAARLHRLGFPESVPLLEFVRRFGLLAGDLASNKDVSVEQILAVNELDVASYRIGPSQILFRSGVLSELEAKRDVLLSDRIIQLQAFCRGYLARKKMSQRRVQELAVRCIQRNVKAFLAVRDWPWWRLLVRVTPLLNVHRTEEQLKTANEELLMLRAKLEKIECDRSEVKAENQKLEAKLSELTVDLAEERSTAHIATERLEAETAERLKLEKELGDQTNKVKNLQETTEKLEMELICAKSDLNGISEDEDAENEDGVGGGVYKLKYERVARELEFTKRRLHTQHEHDLEQLVALKKHLEMKLSDAYEEVVEQRQVVGQWKRKAQKMTNEMNDLRMLLEEQNARNNLLEKKQRKFDAECQSLQDAVRQERQAKERYGREKDVLQAEKFTLEQTLADTRLDLEFKEEKLASLQRELEEMTFGGGTEEEFAQLRRSKNETERRAKEQEEELDEMAGQIQLLEQAKLRLEMTLETMRKEARRESQQRDEELEEVRGNGYKKIKALECQLETEHEERTLLLREKHELERRLSSMEDRDRVDRDAEEALNQKLRRDLRKYKALLKDAQTQLERLKADTPGKTLIRQLRNQLEDAESARSLAMKARQTAEAELTEVQAMFDESHRARNDAEERANAAHRDRAELQAQIEENEEELGELMKKYSATVKQLNTEQINVSEAEFKLNEMEAERNNLKEQVAELQHRLDNVENLGDPSMAMMSKRLELRTKELESRLELEQATRARLEVQVNRHKEALEKLQNEVTQSKMREMQAQDVIKKSQKSLRDMREEFHAVSSREQESLTRRKDLEKKVEQMESEGAALKNDLRLALQRIADLQQAMEEEGEEELSESDESLSSVGSISDLEDRLRPVHVKRSSQQSLNGSIGGGGGSVVSSTRTVVFEKDDNSPRYADNEVNSNSRKQHKH

Target ORF information:

RefSeq Version	NM_001043251.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain-like, transcript variant F (Mhcl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043251.2

ORF Insert Sequence:

ATGTTCCTCA AGCCGAAAAG CTTTGAACAG TCGCTGCGCG GCGGTATCGT TCCGGCTGTG 
CCAGCCGCTC CAGGATCGGG CCTCAATCCA AATTTCCATA AGGGTTGTAC GGGCAAGGCG 
CATGCCTGCG GCACAAACAA ATCCCATCGA GCCGGAGGAT CAGCGACTTC AGCGTCTGGA 
GGATCCAGGG CTGGCAGACA GACGCCTAAG GCTCAGAGAA AGAATAGCAT CAGTTCGGAG 
ACGATTAGCG AAAGTGGTTC GGAGAACTCC AACAGCTGGC CATCCTCCTC ATCGGCAGCC 
GTGCCAGCTA CCGTTCCGCC ACCGAAACCT CCGCGTCTGG CGGCCACTAA AGCTAAGGAA 
GCCGCTGCCA TCGACTGCTC TCCCGTACAG CCTCCTTTTA CCCCAACCTT CGAGCGCTCC 
ACGAAACGCT CGCTCAGCTT CAGGAGCAAC AACAGCAAGA CAACGGAGGC GAAGCTTACT 
CCGGGAGCCA GTTCCTCTAT CCGTCCTCCG TGGAATATTT CCGCCGCCGC TGCATCAAAG 
TACGCCGCTG CAGCCGCCAG TGCTCCACAG TCGCCGAATC CTAGCACGAG GAGCTCTTTC 
CGACGACGCC GTGATGCCCA GAACGCTGGT CTCCATTGGC CGGCCATTTT GGCCAGTTCC 
CTGGCCGCCA GCCATAAAAA CTACGATGCA ATTTTAAAGC AAAAGAGCCG CCATGAGAAT 
GGCAATGGCG GAGGAGAAGA GGGAGGAGCC AAAGGAGTGG GCCACGATCT GGAGGCGGAT 
GTTGCGGATG CCAGTCAGCC GGAGCGCGTA TGGCTGGTGC ACCGCGGTGG TTTCACTGCC 
GCCATCCGTC TGCCGACTGC GTCTTCTGGC CGGGATGAGG AGAACAAGCT AAGCCTGCGC 
CTCCTGCATA ATGGGGAGCA GCTGACCGTC GACGAGGACG ATGTCGAGAA GCAGAACAGC 
CCCGCCCTGG ACCTTGTCGA GGACATCTGC GAGCTGAAGT ATCTGAACGA GGCGTCCGTG 
CTCCATTGCC TGCGTCAGCG ATACGCCAGC AATCTCATCC ACACCAAGGC GGGGCCCACG 
CTGCTCGTGG TCAATCCCAT GGCACCGCTG TCCCTGTACT CCGAGAAGGT TGTCTCCATG 
TTCCGCGGCT GCAAGACGGA GGATATGCCG CCCCACGTCT ATTCACTGGC CCAGACCGCC 
TACAGGAGCC TGGTCGAGAC GCGGCGCGAC CAGAGCTTGA TATTCATGGG CCGATCTGGT 
TCTGGCAAAT CCACTAGCTT CAAGCATGCA CTCAACTACC TGGCGTTGGC AGCTGGTGCC 
TATAATAATT TTATAAACGC GGAGAAGGTC AATGCCCTGT GCACCATTCT GGAGGCTTTT 
GGAAATACGA AAACTTGCCT AAACTCGAAT GCCACGCGGA TGACGCAGCT GTTAAGCCTG 
GATTTCGATC AAACCGGCCA GATTGCATCT GCCTCATTGC AAGTTCTCCT CCCAGAAAGA 
CAACGAGCTG GCCGACGACT TGGTCATGAA CATAGCTTCC ACATCATGAC GAGACTTTTG 
GCTGGCGCTG CTGGCTTGCT ACAGAAGGAG CTGCACCTGG AGAACATAAC CTCTGAGGAC 
AGCCATCCTT TTATATCGTT ATCCCAAAAG CTGGAAGATC GGCATCGAGC TGCCAATGAC 
TTTATGCGCA CTGTTCAGGC CTTTGAGACG CTGAACATTG ATGCGAAGGC GGTACGTGGG 
ATCTGGAGCA TTTTGGCGGC CATTTACCAT CTGGGCATTG CTGGCGTCAC AAAACTGGGC 
ACTGGCTCCA CAGCCCGAAC TCAGTTTGCC AATCCCACGG CCGCCCGAAA GGCCTCGGGC 
TTGTTGGGCG TGAATCTGGA GGATCTGTCA TCAGCTGCCT TTGGTCTTAC CCAACCAAAC 
GCCCCCAATG GCGGTCTGAG TCCATCGAAA TCGCCCACCT CGGACACTGG ACACGAATGG 
GCCTGGGAAT GTCTCGAGGC GTTGGTCATT GGTCTATATT CGGAGGCCTT GGCTGCAGTG 
GTGGCATTGA TCAATCGTCA GATCTGCACA TCGTCCCACA CCATAGCCTC AATTATGCTA 
ATTGACACGC CCGGCTTCCA AAATCCAGCC AGCTGTGGCC AGCAAGTGGG TGCTACTCTA 
GCGGATCTGC GTCACAACTA TCTACAGGAG CGTCTACAGA TGCTCTTCCA TCACACCACA 
CTGGTGGCTC CCCGCGATCG CTACGCCCAG GAGCTGGTAG AAATCGAGAT GGACCTGGCA 
TCGGAGTGTC ATCCGGGACC GTTGATCTCG CTTATCGACA AGGCACCTCA GAATCATGTA 
GTGCGCTCGT CCCAGCGAGA TTTGCGAGAG CATGATCGTC GAGGAATGTT GTGGCTACTG 
GATGAGGAAG CTATCTACCC CAACTCCAAC GATGACACAT TCCTTGAACG TTTGTTCTCC 
CACTACGGTG ACCGGGAACA TCATAGCCTG CTGCGCAAAT GTGCAGGTCC GCGACAATTT 
GTACTCCACC ATCTGCAAGG CACCAATCCC GTTCTCTACG CTGTCGATGG ATGGGTGCGT 
CATAGCCGGG AGCACCCAGG TATTCGGAAT GCTGTATCCC TACTACAGGA CAGCAGTCGG 
GAGGAGATCA ATCGCCTATA TATCGGCTCA CTGACTCGAG GATCGGGAGC AATGGTGTTT 
TGTGGCTCTT TTGCTGGACT GGAAGGCACT CAGTCACTTC GTCGCGTGTC CAGTATTCGC 
CGCTCCTTCA CAACGGCCGG AGTAAAGCGA AACTCCATAA TGCTGCAGGT GAAGTTCACA 
GTGGATGGAA TTATCGACAC TTTGCGCCGC ACTGGAACTC ACTTTGTGCA CTGCTATCTG 
CTGCAACATA ACGCAGGCAA ACATACCAAG TACACCGCCA ATGGATCGCC CAGCTCAGCT 
GCCGGTCAGG TGTCCAGCGA GGAGGAAATG GTCAACGTGC CACTGTTAAG GAGTCAGCTA 
CGTGGTTCAC AAGTCCTAGA AGCGGCTCGC TTGCATCGCC TTGGTTTTCC CGAATCAGTG 
CCATTGTTGG AATTTGTCAG GCGTTTCGGG CTTTTGGCCG GGGATTTGGC TAGCAATAAG 
GATGTGAGCG TGGAGCAGAT ATTAGCTGTC AATGAACTGG ATGTGGCCAG CTATCGCATA 
GGACCCAGCC AGATCCTCTT CCGCTCAGGA GTTCTTAGTG AACTGGAAGC CAAGCGCGAT 
GTCCTGCTCT CAGATCGCAT TATACAGCTG CAAGCATTTT GCCGCGGCTA CTTGGCACGC 
AAGAAGATGT CCCAACGAAG GGTCCAGGAA CTGGCAGTGC GTTGCATTCA ACGCAATGTG 
AAGGCATTCC TTGCCGTTCG CGACTGGCCG TGGTGGCGTC TCCTGGTGCG GGTCACTCCC 
CTGCTCAACG TTCATCGCAC CGAGGAGCAG CTGAAGACGG CTAACGAGGA GCTCCTAATG 
CTGCGAGCCA AGCTGGAGAA GATCGAGTGT GATCGCAGTG AGGTCAAGGC AGAAAACCAA 
AAGCTGGAAG CCAAGCTATC CGAGCTGACG GTGGACCTGG CCGAGGAGCG ATCCACGGCT 
CATATAGCTA CCGAGCGGCT GGAGGCGGAA ACCGCCGAAC GCTTGAAGCT GGAGAAGGAG 
CTGGGCGATC AAACCAACAA GGTGAAGAAC CTTCAGGAGA CGACGGAGAA GCTGGAAATG 
GAGCTGATAT GCGCCAAGTC CGATCTGAAT GGCATCTCTG AGGACGAGGA TGCAGAGAAC 
GAGGATGGGG TCGGCGGCGG CGTCTACAAG CTTAAGTACG AGCGGGTGGC CAGGGAGCTG 
GAGTTCACCA AAAGGCGGTT ACACACGCAG CATGAGCACG ATCTGGAACA GCTGGTCGCG 
CTCAAGAAGC ATTTGGAGAT GAAGCTTTCC GATGCCTACG AAGAAGTTGT GGAACAACGT 
CAGGTTGTGG GCCAATGGAA GCGCAAGGCA CAGAAGATGA CCAACGAGAT GAACGATCTG 
CGCATGCTGC TCGAGGAGCA AAATGCGCGC AACAATTTGC TCGAGAAGAA GCAGCGCAAG 
TTCGACGCCG AGTGCCAGTC CCTGCAGGAT GCAGTGCGTC AGGAGCGACA GGCCAAAGAG 
CGCTACGGTC GCGAAAAGGA CGTCCTGCAG GCCGAGAAGT TCACACTGGA GCAAACGCTG 
GCGGACACCC GTCTGGATCT TGAATTCAAG GAGGAGAAAC TTGCATCGCT ACAGCGCGAG 
CTGGAGGAGA TGACCTTTGG TGGCGGCACC GAGGAGGAGT TCGCCCAACT GCGGCGCTCC 
AAAAATGAAA CAGAGCGTCG GGCCAAGGAA CAGGAGGAAG AACTGGACGA GATGGCCGGT 
CAGATACAGC TGCTCGAACA AGCCAAGCTT CGCCTGGAGA TGACCCTGGA AACAATGCGC 
AAAGAAGCGC GCCGTGAGTC CCAGCAGCGC GACGAAGAGC TGGAAGAAGT ACGCGGTAAC 
GGCTACAAGA AGATTAAAGC CCTTGAATGC CAGCTGGAAA CTGAGCACGA GGAGCGGACT 
CTGCTGCTGC GCGAGAAGCA CGAGCTAGAG CGACGTCTCT CCTCCATGGA GGATCGCGAT 
CGTGTTGATC GCGATGCCGA GGAAGCGCTC AACCAAAAAC TGCGACGCGA TCTTCGCAAA 
TACAAGGCCC TGCTCAAGGA CGCCCAGACA CAGTTGGAGC GGCTTAAGGC TGACACGCCT 
GGCAAGACGC TTATAAGACA ACTGCGTAAT CAACTGGAGG ATGCCGAATC TGCTCGTTCG 
CTGGCTATGA AAGCGCGACA AACTGCGGAA GCCGAACTTA CTGAAGTGCA GGCCATGTTC 
GATGAGTCGC ATCGAGCTAG GAATGACGCG GAGGAGCGAG CCAATGCCGC ACACAGAGAT 
CGGGCTGAGC TGCAGGCCCA GATCGAGGAG AACGAGGAGG AGCTGGGCGA GCTGATGAAG 
AAGTACAGCG CCACGGTAAA GCAACTAAAT ACAGAGCAAA TCAACGTATC CGAGGCTGAG 
TTTAAACTCA ATGAAATGGA AGCAGAGCGC AATAACCTCA AGGAGCAGGT GGCCGAGCTG 
CAGCACCGAC TGGACAACGT TGAGAACTTG GGGGATCCAT CTATGGCCAT GATGTCCAAG 
CGACTGGAGC TGCGCACCAA GGAATTAGAG TCTCGACTGG AACTAGAGCA GGCCACTCGG 
GCTCGCCTTG AAGTGCAGGT GAACCGTCAC AAAGAGGCTC TGGAAAAACT GCAGAACGAG 
GTGACGCAGT CAAAGATGCG TGAGATGCAG GCCCAGGATG TAATCAAAAA ATCGCAAAAG 
AGTCTGCGCG ACATGCGCGA AGAGTTCCAC GCTGTCTCCA GCCGCGAGCA GGAGTCGCTC 
ACAAGGCGCA AGGACCTGGA GAAGAAGGTG GAGCAAATGG AGTCGGAGGG AGCGGCGCTT 
AAAAACGATC TACGACTGGC TTTACAGCGA ATAGCTGATC TACAGCAGGC AATGGAGGAA 
GAGGGCGAGG AGGAGCTGAG CGAAAGTGAT GAAAGCCTCA GTTCGGTGGG CTCTATCAGT 
GATCTGGAAG ATCGACTTCG GCCAGTTCAT GTAAAACGCA GCTCACAGCA GTCCTTGAAC 
GGCAGCATCG GCGGCGGAGG CGGAAGCGTT GTCAGCTCCA CCCGCACCGT GGTGTTCGAA 
AAGGACGACA ACAGCCCGAG GTACGCTGAT AACGAAGTCA ACTCGAACAG CAGAAAGCAG 
CACAAGCATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhcl ( NM_169700.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732108.2 Drosophila melanogaster myosin heavy chain-like, transcript variant A (Mhcl), mRNA.

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CloneID	OFa05081
Clone ID Related Accession (Same CDS sequence)	NM_169700.3
Accession Version	NM_169700.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain-like, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 31, 2011 this sequence version replaced NM_169700.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCAACT TTATGAAGAA GAGCGCTGGG GATGAGGACA AGGAGCGTCG CAAGCGCGAA 
AAAAAACTGC GCAAAGAGGC TAAGGGCACC TCTGGTGGCC CGGGAATGGG AGGCGTGACT 
GGCGTCACCG GCAGCATGAG CACCGACGAG CTGCTCCGCC TGGACGAGGT GCGCCGATCG 
CTTAAAATCC GAGGGCGACG CAAGGAGAAG GAAAAGTTGC CGAGCGGAAT TACGGCGGAC 
TATAGCGCAG ACTTCTTTGC CGCCCTCAAT GCAGATGCAA ACGGCGGAAG CAACGGAGGA 
GCAGTGGGAG CTGCAGATCC CGAGCAGGAT CGTGGCAATG AGGAGATTGT AGCATCTGTG 
ATGACGCACA TCGAGAGCCG AGCGTCCAAT GGCGCCGGCG TGAGTGTCAA CTACAGCGAG 
GTCACACATT CTCTCCTCTC TGGAGGGCTC AAGAATCGCT TTCTTCCACC TATACCCCCA 
AAACCACCCA AGCGTGGCAT TCTGAAAGGC TCCCGCAGTA ATATGACCAA TGTCCACGAG 
GAGATTAGCT TTGCGGGTAG TGCCGGAGGA GCAGGTGGCA CGCCAGAGAT GCTATTGCGC 
AACACCTTTC AGAATGAATC CCTTTACGAG GGAGCTGCCA GGGGAGCTGG TTCGATCGGC 
TCGAACTCCA GTCAGCATGG AACCTCGTTC ACTTCCGTGG AGAGCCTGCG CAGCGATGGA 
GAGCAGACAG GAGGTCAACA ACGGGCCATG ACCCTGCCGG CCAACGCCCG CTATGGAGCT 
TTACCCCAGG GCAACAGTCA AGGATCGCAC CTCCATGTGC TCACCTCGCC GTCGCCCAGC 
GCCGACAGTC TCACAGATAC CACAAACTCC TCTTTCGCCA CACCCCCATT TTCGCTGAGT 
CCCGTCGGCG AGTCCCAAGG CATCGACCGT TGGGCCCGCG TCCATGCCTT CGAGGATGTG 
GAGCTGCCGC TGCCGCCAGT GCAGCTAGTA AAACTTCCCC CACCAAGGCA GCTAGTGATC 
AGGCGACAGA AGTCTCCGCG CCAGGACTTC GGCTTTAGCC TGCGCAAGGC CATCTGTTTG 
GACAGAACAG AGTCCCTGAC TTCGCCCATT TTCAGGCCGG TTATATTCGC CGAGCCAGGT 
GCTGGTGGCG GAGCCACAGG TCTTTTGCCT GGCGATCGCC TAATCAAGGT CAACGGCACG 
CCGGTTGGGG AACTGCCGCG GGAGATCATA ATTGAGATGA TACGGAACAG CGGAGAGGCT 
GTCACCGTGG AGGTCCAACC AGTCGCTGAG TTGGTGGAGC TGAGCAAGCG ATGCATGGCC 
CCCAGCACAG CCACCGTGGA GGAGATCGAT CATTCCATTA CCAACGGCAA CTGCAATACC 
CTGCGACGCA GTGCCAGCAA GCGCTTCAAG CGTCAGAGCC GCCATGAGAA TGGCAATGGC 
GGAGGAGAAG AGGGAGGAGC CAAAGGAGTG GGCCACGATC TGGAGGCGGA TGTTGCGGAT 
GCCAGTCAGC CGGAGCGCGT ATGGCTGGTG CACCGCGGTG GTTTCACTGC CGCCATCCGT 
CTGCCGACTG CGTCTTCTGG CCGGGATGAG GAGAACAAGC TAAGCCTGCG CCTCCTGCAT 
AATGGGGAGC AGCTGACCGT CGACGAGGAC GATGTCGAGA AGCAGAACAG CCCCGCCCTG 
GACCTTGTCG AGGACATCTG CGAGCTGAAG TATCTGAACG AGGCGTCCGT GCTCCATTGC 
CTGCGTCAGC GATACGCCAG CAATCTCATC CACACCAAGG CGGGGCCCAC GCTGCTCGTG 
GTCAATCCCA TGGCACCGCT GTCCCTGTAC TCCGAGAAGG TTGTCTCCAT GTTCCGCGGC 
TGCAAGACGG AGGATATGCC GCCCCACGTC TATTCACTGG CCCAGACCGC CTACAGGAGC 
CTGGTCGAGA CGCGGCGCGA CCAGAGCTTG ATATTCATGG GCCGATCTGG TTCTGGCAAA 
TCCACTAGCT TCAAGCATGC ACTCAACTAC CTGGCGTTGG CAGCTGGTGC CTATAATAAT 
TTTATAAACG CGGAGAAGGT CAATGCCCTG TGCACCATTC TGGAGGCTTT TGGAAATACG 
AAAACTTGCC TAAACTCGAA TGCCACGCGG ATGACGCAGC TGTTAAGCCT GGATTTCGAT 
CAAACCGGCC AGATTGCATC TGCCTCATTG CAAGTTCTCC TCCCAGAAAG ACAACGAGCT 
GGCCGACGAC TTGGTCATGA ACATAGCTTC CACATCATGA CGAGACTTTT GGCTGGCGCT 
GCTGGCTTGC TACAGAAGGA GCTGCACCTG GAGAACATAA CCTCTGAGGA CAGCCATCCT 
TTTATATCGT TATCCCAAAA GCTGGAAGAT CGGCATCGAG CTGCCAATGA CTTTATGCGC 
ACTGTTCAGG CCTTTGAGAC GCTGAACATT GATGCGAAGG CGGTACGTGG GATCTGGAGC 
ATTTTGGCGG CCATTTACCA TCTGGGCATT GCTGGCGTCA CAAAACTGGG CACTGGCTCC 
ACAGCCCGAA CTCAGTTTGC CAATCCCACG GCCGCCCGAA AGGCCTCGGG CTTGTTGGGC 
GTGAATCTGG AGGATCTGTC ATCAGCTGCC TTTGGTCTTA CCCAACCAAA CGCCCCCAAT 
GGCGGTCTGA GTCCATCGAA ATCGCCCACC TCGGACACTG GACACGAATG GGCCTGGGAA 
TGTCTCGAGG CGTTGGTCAT TGGTCTATAT TCGGAGGCCT TGGCTGCAGT GGTGGCATTG 
ATCAATCGTC AGATCTGCAC ATCGTCCCAC ACCATAGCCT CAATTATGCT AATTGACACG 
CCCGGCTTCC AAAATCCAGC CAGCTGTGGC CAGCAAGTGG GTGCTACTCT AGCGGATCTG 
CGTCACAACT ATCTACAGGA GCGTCTACAG ATGCTCTTCC ATCACACCAC ACTGGTGGCT 
CCCCGCGATC GCTACGCCCA GGAGCTGGTA GAAATCGAGA TGGACCTGGC ATCGGAGTGT 
CATCCGGGAC CGTTGATCTC GCTTATCGAC AAGGCACCTC AGAATCATGT AGTGCGCTCG 
TCCCAGCGAG ATTTGCGAGA GCATGATCGT CGAGGAATGT TGTGGCTACT GGATGAGGAA 
GCTATCTACC CCAACTCCAA CGATGACACA TTCCTTGAAC GTTTGTTCTC CCACTACGGT 
GACCGGGAAC ATCATAGCCT GCTGCGCAAA TGTGCAGGTC CGCGACAATT TGTACTCCAC 
CATCTGCAAG GCACCAATCC CGTTCTCTAC GCTGTCGATG GATGGGTGCG TCATAGCCGG 
GAGCACCCAG GTATTCGGAA TGCTGTATCC CTACTACAGG ACAGCAGTCG GGAGGAGATC 
AATCGCCTAT ATATCGGCTC ACTGACTCGA GGATCGGGAG CAATGGTGTT TTGTGGCTCT 
TTTGCTGGAC TGGAAGGCAC TCAGTCACTT CGTCGCGTGT CCAGTATTCG CCGCTCCTTC 
ACAACGGCCG GAGTAAAGCG AAACTCCATA ATGCTGCAGG TGAAGTTCAC AGTGGATGGA 
ATTATCGACA CTTTGCGCCG CACTGGAACT CACTTTGTGC ACTGCTATCT GCTGCAACAT 
AACGCAGGCA AACATACCAA GTACACCGCC AATGGATCGC CCAGCTCAGC TGCCGGTCAG 
GTGTCCAGCG AGGAGGAAAT GGTCAACGTG CCACTGTTAA GGAGTCAGCT ACGTGGTTCA 
CAAGTCCTAG AAGCGGCTCG CTTGCATCGC CTTGGTTTTC CCGAATCAGT GCCATTGTTG 
GAATTTGTCA GGCGTTTCGG GCTTTTGGCC GGGGATTTGG CTAGCAATAA GGATGTGAGC 
GTGGAGCAGA TATTAGCTGT CAATGAACTG GATGTGGCCA GCTATCGCAT AGGACCCAGC 
CAGATCCTCT TCCGCTCAGG AGTTCTTAGT GAACTGGAAG CCAAGCGCGA TGTCCTGCTC 
TCAGATCGCA TTATACAGCT GCAAGCATTT TGCCGCGGCT ACTTGGCACG CAAGAAGATG 
TCCCAACGAA GGGTCCAGGA ACTGGCAGTG CGTTGCATTC AACGCAATGT GAAGGCATTC 
CTTGCCGTTC GCGACTGGCC GTGGTGGCGT CTCCTGGTGC GGGTCACTCC CCTGCTCAAC 
GTTCATCGCA CCGAGGAGCA GCTGAAGACG GCTAACGAGG AGCTCCTAAT GCTGCGAGCC 
AAGCTGGAGA AGATCGAGTG TGATCGCAGT GAGGTCAAGG CAGAAAACCA AAAGCTGGAA 
GCCAAGCTAT CCGAGCTGAC GGTGGACCTG GCCGAGGAGC GATCCACGGC TCATATAGCT 
ACCGAGCGGC TGGAGGCGGA AACCGCCGAA CGCTTGAAGC TGGAGAAGGA GCTGGGCGAT 
CAAACCAACA AGGTGAAGAA CCTTCAGGAG ACGACGGAGA AGCTGGAAAT GGAGCTGATA 
TGCGCCAAGT CCGATCTGAA TGGCATCTCT GAGGACGAGG ATGCAGAGAA CGAGGATGGG 
GTCGGCGGCG GCGTCTACAA GCTTAAGTAC GAGCGGGTGG CCAGGGAGCT GGAGTTCACC 
AAAAGGCGGT TACACACGCA GCATGAGCAC GATCTGGAAC AGCTGGTCGC GCTCAAGAAG 
CATTTGGAGA TGAAGCTTTC CGATGCCTAC GAAGAAGTTG TGGAACAACG TCAGGTTGTG 
GGCCAATGGA AGCGCAAGGC ACAGAAGATG ACCAACGAGA TGAACGATCT GCGCATGCTG 
CTCGAGGAGC AAAATGCGCG CAACAATTTG CTCGAGAAGA AGCAGCGCAA GTTCGACGCC 
GAGTGCCAGT CCCTGCAGGA TGCAGTGCGT CAGGAGCGAC AGGCCAAAGA GCGCTACGGT 
CGCGAAAAGG ACGTCCTGCA GGCCGAGAAG TTCACACTGG AGCAAACGCT GGCGGACACC 
CGTCTGGATC TTGAATTCAA GGAGGAGAAA CTTGCATCGC TACAGCGCGA GCTGGAGGAG 
ATGACCTTTG GTGGCGGCAC CGAGGAGGAG TTCGCCCAAC TGCGGCGCTC CAAAAATGAA 
ACAGAGCGTC GGGCCAAGGA ACAGGAGGAA GAACTGGACG AGATGGCCGG TCAGATACAG 
CTGCTCGAAC AAGCCAAGCT TCGCCTGGAG ATGACCCTGG AAACAATGCG CAAAGAAGCG 
CGCCGTGAGT CCCAGCAGCG CGACGAAGAG CTGGAAGAAG TACGCGGTAA CGGCTACAAG 
AAGATTAAAG CCCTTGAATG CCAGCTGGAA ACTGAGCACG AGGAGCGGAC TCTGCTGCTG 
CGCGAGAAGC ACGAGCTAGA GCGACGTCTC TCCTCCATGG AGGATCGCGA TCGTGTTGAT 
CGCGATGCCG AGGAAGCGCT CAACCAAAAA CTGCGACGCG ATCTTCGCAA ATACAAGGCC 
CTGCTCAAGG ACGCCCAGAC ACAGTTGGAG CGGCTTAAGG CTGACACGCC TGGCAAGACG 
CTTATAAGAC AACTGCGTAA TCAACTGGAG GATGCCGAAT CTGCTCGTTC GCTGGCTATG 
AAAGCGCGAC AAACTGCGGA AGCCGAACTT ACTGAAGTGC AGGCCATGTT CGATGAGTCG 
CATCGAGCTA GGAATGACGC GGAGGAGCGA GCCAATGCCG CACACAGAGA TCGGGCTGAG 
CTGCAGGCCC AGATCGAGGA GAACGAGGAG GAGCTGGGCG AGCTGATGAA GAAGTACAGC 
GCCACGGTAA AGCAACTAAA TACAGAGCAA ATCAACGTAT CCGAGGCTGA GTTTAAACTC 
AATGAAATGG AAGCAGAGCG CAATAACCTC AAGGAGCAGG TGGCCGAGCT GCAGCACCGA 
CTGGACAACG TTGAGAACTT GGGGGATCCA TCTATGGCCA TGATGTCCAA GCGACTGGAG 
CTGCGCACCA AGGAATTAGA GTCTCGACTG GAACTAGAGC AGGCCACTCG GGCTCGCCTT 
GAAGTGCAGG TGAACCGTCA CAAAGAGGCT CTGGAAAAAC TGCAGAACGA GGTGACGCAG 
TCAAAGATGC GTGAGATGCA GGCCCAGGAT GTAATCAAAA AATCGCAAAA GAGTCTGCGC 
GACATGCGCG AAGAGTTCCA CGCTGTCTCC AGCCGCGAGC AGGAGTCGCT CACAAGGCGC 
AAGGACCTGG AGAAGAAGGT GGAGCAAATG GAGTCGGAGG GAGCGGCGCT TAAAAACGAT 
CTACGACTGG CTTTACAGCG AATAGCTGAT CTACAGCAGG CAATGGAGGA AGAGGGCGAG 
GAGGAGCTGA GCGAAAGTGA TGAAAGCCTC AGTTCGGTGG GCTCTATCAG TGATCTGGAA 
GATCGACTTC GGCCAGTTCA TGTAAAACGC AGCTCACAGC AGTCCTTGAA CGGCAGCATC 
GGCGGCGGAG GCGGAAGCGT TGTCAGCTCC ACCCGCACCG TGGTGTTCGA AAAGGACGAC 
AACAGCCCGA GAAATAACGC CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732108.2
CDS	164..6610
Translation	MFNFMKKSAGDEDKERRKREKKLRKEAKGTSGGPGMGGVTGVTGSMSTDELLRLDEVRRSLKIRGRRKEKEKLPSGITADYSADFFAALNADANGGSNGGAVGAADPEQDRGNEEIVASVMTHIESRASNGAGVSVNYSEVTHSLLSGGLKNRFLPPIPPKPPKRGILKGSRSNMTNVHEEISFAGSAGGAGGTPEMLLRNTFQNESLYEGAARGAGSIGSNSSQHGTSFTSVESLRSDGEQTGGQQRAMTLPANARYGALPQGNSQGSHLHVLTSPSPSADSLTDTTNSSFATPPFSLSPVGESQGIDRWARVHAFEDVELPLPPVQLVKLPPPRQLVIRRQKSPRQDFGFSLRKAICLDRTESLTSPIFRPVIFAEPGAGGGATGLLPGDRLIKVNGTPVGELPREIIIEMIRNSGEAVTVEVQPVAELVELSKRCMAPSTATVEEIDHSITNGNCNTLRRSASKRFKRQSRHENGNGGGEEGGAKGVGHDLEADVADASQPERVWLVHRGGFTAAIRLPTASSGRDEENKLSLRLLHNGEQLTVDEDDVEKQNSPALDLVEDICELKYLNEASVLHCLRQRYASNLIHTKAGPTLLVVNPMAPLSLYSEKVVSMFRGCKTEDMPPHVYSLAQTAYRSLVETRRDQSLIFMGRSGSGKSTSFKHALNYLALAAGAYNNFINAEKVNALCTILEAFGNTKTCLNSNATRMTQLLSLDFDQTGQIASASLQVLLPERQRAGRRLGHEHSFHIMTRLLAGAAGLLQKELHLENITSEDSHPFISLSQKLEDRHRAANDFMRTVQAFETLNIDAKAVRGIWSILAAIYHLGIAGVTKLGTGSTARTQFANPTAARKASGLLGVNLEDLSSAAFGLTQPNAPNGGLSPSKSPTSDTGHEWAWECLEALVIGLYSEALAAVVALINRQICTSSHTIASIMLIDTPGFQNPASCGQQVGATLADLRHNYLQERLQMLFHHTTLVAPRDRYAQELVEIEMDLASECHPGPLISLIDKAPQNHVVRSSQRDLREHDRRGMLWLLDEEAIYPNSNDDTFLERLFSHYGDREHHSLLRKCAGPRQFVLHHLQGTNPVLYAVDGWVRHSREHPGIRNAVSLLQDSSREEINRLYIGSLTRGSGAMVFCGSFAGLEGTQSLRRVSSIRRSFTTAGVKRNSIMLQVKFTVDGIIDTLRRTGTHFVHCYLLQHNAGKHTKYTANGSPSSAAGQVSSEEEMVNVPLLRSQLRGSQVLEAARLHRLGFPESVPLLEFVRRFGLLAGDLASNKDVSVEQILAVNELDVASYRIGPSQILFRSGVLSELEAKRDVLLSDRIIQLQAFCRGYLARKKMSQRRVQELAVRCIQRNVKAFLAVRDWPWWRLLVRVTPLLNVHRTEEQLKTANEELLMLRAKLEKIECDRSEVKAENQKLEAKLSELTVDLAEERSTAHIATERLEAETAERLKLEKELGDQTNKVKNLQETTEKLEMELICAKSDLNGISEDEDAENEDGVGGGVYKLKYERVARELEFTKRRLHTQHEHDLEQLVALKKHLEMKLSDAYEEVVEQRQVVGQWKRKAQKMTNEMNDLRMLLEEQNARNNLLEKKQRKFDAECQSLQDAVRQERQAKERYGREKDVLQAEKFTLEQTLADTRLDLEFKEEKLASLQRELEEMTFGGGTEEEFAQLRRSKNETERRAKEQEEELDEMAGQIQLLEQAKLRLEMTLETMRKEARRESQQRDEELEEVRGNGYKKIKALECQLETEHEERTLLLREKHELERRLSSMEDRDRVDRDAEEALNQKLRRDLRKYKALLKDAQTQLERLKADTPGKTLIRQLRNQLEDAESARSLAMKARQTAEAELTEVQAMFDESHRARNDAEERANAAHRDRAELQAQIEENEEELGELMKKYSATVKQLNTEQINVSEAEFKLNEMEAERNNLKEQVAELQHRLDNVENLGDPSMAMMSKRLELRTKELESRLELEQATRARLEVQVNRHKEALEKLQNEVTQSKMREMQAQDVIKKSQKSLRDMREEFHAVSSREQESLTRRKDLEKKVEQMESEGAALKNDLRLALQRIADLQQAMEEEGEEELSESDESLSSVGSISDLEDRLRPVHVKRSSQQSLNGSIGGGGGSVVSSTRTVVFEKDDNSPRNNAK

Target ORF information:

RefSeq Version	NM_169700.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain-like, transcript variant A (Mhcl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169700.3

ORF Insert Sequence:

ATGTTCAACT TTATGAAGAA GAGCGCTGGG GATGAGGACA AGGAGCGTCG CAAGCGCGAA 
AAAAAACTGC GCAAAGAGGC TAAGGGCACC TCTGGTGGCC CGGGAATGGG AGGCGTGACT 
GGCGTCACCG GCAGCATGAG CACCGACGAG CTGCTCCGCC TGGACGAGGT GCGCCGATCG 
CTTAAAATCC GAGGGCGACG CAAGGAGAAG GAAAAGTTGC CGAGCGGAAT TACGGCGGAC 
TATAGCGCAG ACTTCTTTGC CGCCCTCAAT GCAGATGCAA ACGGCGGAAG CAACGGAGGA 
GCAGTGGGAG CTGCAGATCC CGAGCAGGAT CGTGGCAATG AGGAGATTGT AGCATCTGTG 
ATGACGCACA TCGAGAGCCG AGCGTCCAAT GGCGCCGGCG TGAGTGTCAA CTACAGCGAG 
GTCACACATT CTCTCCTCTC TGGAGGGCTC AAGAATCGCT TTCTTCCACC TATACCCCCA 
AAACCACCCA AGCGTGGCAT TCTGAAAGGC TCCCGCAGTA ATATGACCAA TGTCCACGAG 
GAGATTAGCT TTGCGGGTAG TGCCGGAGGA GCAGGTGGCA CGCCAGAGAT GCTATTGCGC 
AACACCTTTC AGAATGAATC CCTTTACGAG GGAGCTGCCA GGGGAGCTGG TTCGATCGGC 
TCGAACTCCA GTCAGCATGG AACCTCGTTC ACTTCCGTGG AGAGCCTGCG CAGCGATGGA 
GAGCAGACAG GAGGTCAACA ACGGGCCATG ACCCTGCCGG CCAACGCCCG CTATGGAGCT 
TTACCCCAGG GCAACAGTCA AGGATCGCAC CTCCATGTGC TCACCTCGCC GTCGCCCAGC 
GCCGACAGTC TCACAGATAC CACAAACTCC TCTTTCGCCA CACCCCCATT TTCGCTGAGT 
CCCGTCGGCG AGTCCCAAGG CATCGACCGT TGGGCCCGCG TCCATGCCTT CGAGGATGTG 
GAGCTGCCGC TGCCGCCAGT GCAGCTAGTA AAACTTCCCC CACCAAGGCA GCTAGTGATC 
AGGCGACAGA AGTCTCCGCG CCAGGACTTC GGCTTTAGCC TGCGCAAGGC CATCTGTTTG 
GACAGAACAG AGTCCCTGAC TTCGCCCATT TTCAGGCCGG TTATATTCGC CGAGCCAGGT 
GCTGGTGGCG GAGCCACAGG TCTTTTGCCT GGCGATCGCC TAATCAAGGT CAACGGCACG 
CCGGTTGGGG AACTGCCGCG GGAGATCATA ATTGAGATGA TACGGAACAG CGGAGAGGCT 
GTCACCGTGG AGGTCCAACC AGTCGCTGAG TTGGTGGAGC TGAGCAAGCG ATGCATGGCC 
CCCAGCACAG CCACCGTGGA GGAGATCGAT CATTCCATTA CCAACGGCAA CTGCAATACC 
CTGCGACGCA GTGCCAGCAA GCGCTTCAAG CGTCAGAGCC GCCATGAGAA TGGCAATGGC 
GGAGGAGAAG AGGGAGGAGC CAAAGGAGTG GGCCACGATC TGGAGGCGGA TGTTGCGGAT 
GCCAGTCAGC CGGAGCGCGT ATGGCTGGTG CACCGCGGTG GTTTCACTGC CGCCATCCGT 
CTGCCGACTG CGTCTTCTGG CCGGGATGAG GAGAACAAGC TAAGCCTGCG CCTCCTGCAT 
AATGGGGAGC AGCTGACCGT CGACGAGGAC GATGTCGAGA AGCAGAACAG CCCCGCCCTG 
GACCTTGTCG AGGACATCTG CGAGCTGAAG TATCTGAACG AGGCGTCCGT GCTCCATTGC 
CTGCGTCAGC GATACGCCAG CAATCTCATC CACACCAAGG CGGGGCCCAC GCTGCTCGTG 
GTCAATCCCA TGGCACCGCT GTCCCTGTAC TCCGAGAAGG TTGTCTCCAT GTTCCGCGGC 
TGCAAGACGG AGGATATGCC GCCCCACGTC TATTCACTGG CCCAGACCGC CTACAGGAGC 
CTGGTCGAGA CGCGGCGCGA CCAGAGCTTG ATATTCATGG GCCGATCTGG TTCTGGCAAA 
TCCACTAGCT TCAAGCATGC ACTCAACTAC CTGGCGTTGG CAGCTGGTGC CTATAATAAT 
TTTATAAACG CGGAGAAGGT CAATGCCCTG TGCACCATTC TGGAGGCTTT TGGAAATACG 
AAAACTTGCC TAAACTCGAA TGCCACGCGG ATGACGCAGC TGTTAAGCCT GGATTTCGAT 
CAAACCGGCC AGATTGCATC TGCCTCATTG CAAGTTCTCC TCCCAGAAAG ACAACGAGCT 
GGCCGACGAC TTGGTCATGA ACATAGCTTC CACATCATGA CGAGACTTTT GGCTGGCGCT 
GCTGGCTTGC TACAGAAGGA GCTGCACCTG GAGAACATAA CCTCTGAGGA CAGCCATCCT 
TTTATATCGT TATCCCAAAA GCTGGAAGAT CGGCATCGAG CTGCCAATGA CTTTATGCGC 
ACTGTTCAGG CCTTTGAGAC GCTGAACATT GATGCGAAGG CGGTACGTGG GATCTGGAGC 
ATTTTGGCGG CCATTTACCA TCTGGGCATT GCTGGCGTCA CAAAACTGGG CACTGGCTCC 
ACAGCCCGAA CTCAGTTTGC CAATCCCACG GCCGCCCGAA AGGCCTCGGG CTTGTTGGGC 
GTGAATCTGG AGGATCTGTC ATCAGCTGCC TTTGGTCTTA CCCAACCAAA CGCCCCCAAT 
GGCGGTCTGA GTCCATCGAA ATCGCCCACC TCGGACACTG GACACGAATG GGCCTGGGAA 
TGTCTCGAGG CGTTGGTCAT TGGTCTATAT TCGGAGGCCT TGGCTGCAGT GGTGGCATTG 
ATCAATCGTC AGATCTGCAC ATCGTCCCAC ACCATAGCCT CAATTATGCT AATTGACACG 
CCCGGCTTCC AAAATCCAGC CAGCTGTGGC CAGCAAGTGG GTGCTACTCT AGCGGATCTG 
CGTCACAACT ATCTACAGGA GCGTCTACAG ATGCTCTTCC ATCACACCAC ACTGGTGGCT 
CCCCGCGATC GCTACGCCCA GGAGCTGGTA GAAATCGAGA TGGACCTGGC ATCGGAGTGT 
CATCCGGGAC CGTTGATCTC GCTTATCGAC AAGGCACCTC AGAATCATGT AGTGCGCTCG 
TCCCAGCGAG ATTTGCGAGA GCATGATCGT CGAGGAATGT TGTGGCTACT GGATGAGGAA 
GCTATCTACC CCAACTCCAA CGATGACACA TTCCTTGAAC GTTTGTTCTC CCACTACGGT 
GACCGGGAAC ATCATAGCCT GCTGCGCAAA TGTGCAGGTC CGCGACAATT TGTACTCCAC 
CATCTGCAAG GCACCAATCC CGTTCTCTAC GCTGTCGATG GATGGGTGCG TCATAGCCGG 
GAGCACCCAG GTATTCGGAA TGCTGTATCC CTACTACAGG ACAGCAGTCG GGAGGAGATC 
AATCGCCTAT ATATCGGCTC ACTGACTCGA GGATCGGGAG CAATGGTGTT TTGTGGCTCT 
TTTGCTGGAC TGGAAGGCAC TCAGTCACTT CGTCGCGTGT CCAGTATTCG CCGCTCCTTC 
ACAACGGCCG GAGTAAAGCG AAACTCCATA ATGCTGCAGG TGAAGTTCAC AGTGGATGGA 
ATTATCGACA CTTTGCGCCG CACTGGAACT CACTTTGTGC ACTGCTATCT GCTGCAACAT 
AACGCAGGCA AACATACCAA GTACACCGCC AATGGATCGC CCAGCTCAGC TGCCGGTCAG 
GTGTCCAGCG AGGAGGAAAT GGTCAACGTG CCACTGTTAA GGAGTCAGCT ACGTGGTTCA 
CAAGTCCTAG AAGCGGCTCG CTTGCATCGC CTTGGTTTTC CCGAATCAGT GCCATTGTTG 
GAATTTGTCA GGCGTTTCGG GCTTTTGGCC GGGGATTTGG CTAGCAATAA GGATGTGAGC 
GTGGAGCAGA TATTAGCTGT CAATGAACTG GATGTGGCCA GCTATCGCAT AGGACCCAGC 
CAGATCCTCT TCCGCTCAGG AGTTCTTAGT GAACTGGAAG CCAAGCGCGA TGTCCTGCTC 
TCAGATCGCA TTATACAGCT GCAAGCATTT TGCCGCGGCT ACTTGGCACG CAAGAAGATG 
TCCCAACGAA GGGTCCAGGA ACTGGCAGTG CGTTGCATTC AACGCAATGT GAAGGCATTC 
CTTGCCGTTC GCGACTGGCC GTGGTGGCGT CTCCTGGTGC GGGTCACTCC CCTGCTCAAC 
GTTCATCGCA CCGAGGAGCA GCTGAAGACG GCTAACGAGG AGCTCCTAAT GCTGCGAGCC 
AAGCTGGAGA AGATCGAGTG TGATCGCAGT GAGGTCAAGG CAGAAAACCA AAAGCTGGAA 
GCCAAGCTAT CCGAGCTGAC GGTGGACCTG GCCGAGGAGC GATCCACGGC TCATATAGCT 
ACCGAGCGGC TGGAGGCGGA AACCGCCGAA CGCTTGAAGC TGGAGAAGGA GCTGGGCGAT 
CAAACCAACA AGGTGAAGAA CCTTCAGGAG ACGACGGAGA AGCTGGAAAT GGAGCTGATA 
TGCGCCAAGT CCGATCTGAA TGGCATCTCT GAGGACGAGG ATGCAGAGAA CGAGGATGGG 
GTCGGCGGCG GCGTCTACAA GCTTAAGTAC GAGCGGGTGG CCAGGGAGCT GGAGTTCACC 
AAAAGGCGGT TACACACGCA GCATGAGCAC GATCTGGAAC AGCTGGTCGC GCTCAAGAAG 
CATTTGGAGA TGAAGCTTTC CGATGCCTAC GAAGAAGTTG TGGAACAACG TCAGGTTGTG 
GGCCAATGGA AGCGCAAGGC ACAGAAGATG ACCAACGAGA TGAACGATCT GCGCATGCTG 
CTCGAGGAGC AAAATGCGCG CAACAATTTG CTCGAGAAGA AGCAGCGCAA GTTCGACGCC 
GAGTGCCAGT CCCTGCAGGA TGCAGTGCGT CAGGAGCGAC AGGCCAAAGA GCGCTACGGT 
CGCGAAAAGG ACGTCCTGCA GGCCGAGAAG TTCACACTGG AGCAAACGCT GGCGGACACC 
CGTCTGGATC TTGAATTCAA GGAGGAGAAA CTTGCATCGC TACAGCGCGA GCTGGAGGAG 
ATGACCTTTG GTGGCGGCAC CGAGGAGGAG TTCGCCCAAC TGCGGCGCTC CAAAAATGAA 
ACAGAGCGTC GGGCCAAGGA ACAGGAGGAA GAACTGGACG AGATGGCCGG TCAGATACAG 
CTGCTCGAAC AAGCCAAGCT TCGCCTGGAG ATGACCCTGG AAACAATGCG CAAAGAAGCG 
CGCCGTGAGT CCCAGCAGCG CGACGAAGAG CTGGAAGAAG TACGCGGTAA CGGCTACAAG 
AAGATTAAAG CCCTTGAATG CCAGCTGGAA ACTGAGCACG AGGAGCGGAC TCTGCTGCTG 
CGCGAGAAGC ACGAGCTAGA GCGACGTCTC TCCTCCATGG AGGATCGCGA TCGTGTTGAT 
CGCGATGCCG AGGAAGCGCT CAACCAAAAA CTGCGACGCG ATCTTCGCAA ATACAAGGCC 
CTGCTCAAGG ACGCCCAGAC ACAGTTGGAG CGGCTTAAGG CTGACACGCC TGGCAAGACG 
CTTATAAGAC AACTGCGTAA TCAACTGGAG GATGCCGAAT CTGCTCGTTC GCTGGCTATG 
AAAGCGCGAC AAACTGCGGA AGCCGAACTT ACTGAAGTGC AGGCCATGTT CGATGAGTCG 
CATCGAGCTA GGAATGACGC GGAGGAGCGA GCCAATGCCG CACACAGAGA TCGGGCTGAG 
CTGCAGGCCC AGATCGAGGA GAACGAGGAG GAGCTGGGCG AGCTGATGAA GAAGTACAGC 
GCCACGGTAA AGCAACTAAA TACAGAGCAA ATCAACGTAT CCGAGGCTGA GTTTAAACTC 
AATGAAATGG AAGCAGAGCG CAATAACCTC AAGGAGCAGG TGGCCGAGCT GCAGCACCGA 
CTGGACAACG TTGAGAACTT GGGGGATCCA TCTATGGCCA TGATGTCCAA GCGACTGGAG 
CTGCGCACCA AGGAATTAGA GTCTCGACTG GAACTAGAGC AGGCCACTCG GGCTCGCCTT 
GAAGTGCAGG TGAACCGTCA CAAAGAGGCT CTGGAAAAAC TGCAGAACGA GGTGACGCAG 
TCAAAGATGC GTGAGATGCA GGCCCAGGAT GTAATCAAAA AATCGCAAAA GAGTCTGCGC 
GACATGCGCG AAGAGTTCCA CGCTGTCTCC AGCCGCGAGC AGGAGTCGCT CACAAGGCGC 
AAGGACCTGG AGAAGAAGGT GGAGCAAATG GAGTCGGAGG GAGCGGCGCT TAAAAACGAT 
CTACGACTGG CTTTACAGCG AATAGCTGAT CTACAGCAGG CAATGGAGGA AGAGGGCGAG 
GAGGAGCTGA GCGAAAGTGA TGAAAGCCTC AGTTCGGTGG GCTCTATCAG TGATCTGGAA 
GATCGACTTC GGCCAGTTCA TGTAAAACGC AGCTCACAGC AGTCCTTGAA CGGCAGCATC 
GGCGGCGGAG GCGGAAGCGT TGTCAGCTCC ACCCGCACCG TGGTGTTCGA AAAGGACGAC 
AACAGCCCGA GAAATAACGC CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhcl ( NM_169701.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732109.2 Drosophila melanogaster myosin heavy chain-like, transcript variant B (Mhcl), mRNA.

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CloneID	OFa05082
Clone ID Related Accession (Same CDS sequence)	NM_169701.3
Accession Version	NM_169701.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain-like, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 31, 2011 this sequence version replaced NM_169701.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCAACT TTATGAAGAA GAGCGCTGGG GATGAGGACA AGGAGCGTCG CAAGCGCGAA 
AAAAAACTGC GCAAAGAGGC TAAGGGCACC TCTGGTGGCC CGGGAATGGG AGGCGTGACT 
GGCGTCACCG GCAGCATGAG CACCGACGAG CTGCTCCGCC TGGACGAGGT GCGCCGATCG 
CTTAAAATCC GAGGGCGACG CAAGGAGAAG GAAAAGTTGC CGAGCGGAAT TACGGCGGAC 
TATAGCGCAG ACTTCTTTGC CGCCCTCAAT GCAGATGCAA ACGGCGGAAG CAACGGAGGA 
GCAGTGGGAG CTGCAGATCC CGAGCAGGAT CGTGGCAATG AGGAGATTGT AGCATCTGTG 
ATGACGCACA TCGAGAGCCG AGCGTCCAAT GGCGCCGGCG TGAGTGTCAA CTACAGCGAG 
GTCACACATT CTCTCCTCTC TGGAGGGCTC AAGAATCGCT TTCTTCCACC TATACCCCCA 
AAACCACCCA AGCGTGGCAT TCTGAAAGGC TCCCGCAGTA ATATGACCAA TGTCCACGAG 
GAGATTAGCT TTGCGGGTAG TGCCGGAGGA GCAGGTGGCA CGCCAGAGAT GCTATTGCGC 
AACACCTTTC AGAATGAATC CCTTTACGAG GGAGCTGCCA GGGGAGCTGG TTCGATCGGC 
TCGAACTCCA GTCAGCATGG AACCTCGTTC ACTTCCGTGG AGAGCCTGCG CAGCGATGGA 
GAGCAGACAG GAGGTCAACA ACGGGCCATG ACCCTGCCGG CCAACGCCCG CTATGGAGCT 
TTACCCCAGG GCAACAGTCA AGGATCGCAC CTCCATGTGC TCACCTCGCC GTCGCCCAGC 
GCCGACAGTC TCACAGATAC CACAAACTCC TCTTTCGCCA CACCCCCATT TTCGCTGAGT 
CCCGTCGGCG AGTCCCAAGG CATCGACCGT TGGGCCCGCG TCCATGCCTT CGAGGATGTG 
GAGCTGCCGC TGCCGCCAGT GCAGCTAGTA AAACTTCCCC CACCAAGGCA GCTAGTGATC 
AGGCGACAGA AGTCTCCGCG CCAGGACTTC GGCTTTAGCC TGCGCAAGGC CATCTGTTTG 
GACAGAACAG AGTCCCTGAC TTCGCCCATT TTCAGGCCGG TTATATTCGC CGAGCCAGGT 
GCTGGTGGCG GAGCCACAGG TCTTTTGCCT GGCGATCGCC TAATCAAGGT CAACGGCACG 
CCGGTTGGGG AACTGCCGCG GGAGATCATA ATTGAGATGA TACGGAACAG CGGAGAGGCT 
GTCACCGTGG AGGTCCAACC AGTCGCTGAG TTGGTGGAGC TGAGCAAGCG ATGCATGGCC 
CCCAGCACAG CCACCGTGGA GGAGATCGAT CATTCCATTA CCAACGGCAA CTGCAATACC 
CTGCGACGCA GTGCCAGCAA GCGCTTCAAG CGTCAGAGCC GCCATGAGAA TGGCAATGGC 
GGAGGAGAAG AGGGAGGAGC CAAAGGAGTG GGCCACGATC TGGAGGCGGA TGTTGCGGAT 
GCCAGTCAGC CGGAGCGCGT ATGGCTGGTG CACCGCGGTG GTTTCACTGC CGCCATCCGT 
CTGCCGACTG CGTCTTCTGG CCGGGATGAG GAGAACAAGC TAAGCCTGCG CCTCCTGCAT 
AATGGGGAGC AGCTGACCGT CGACGAGGAC GATGTCGAGA AGCAGAACAG CCCCGCCCTG 
GACCTTGTCG AGGACATCTG CGAGCTGAAG TATCTGAACG AGGCGTCCGT GCTCCATTGC 
CTGCGTCAGC GATACGCCAG CAATCTCATC CACACCAAGG CGGGGCCCAC GCTGCTCGTG 
GTCAATCCCA TGGCACCGCT GTCCCTGTAC TCCGAGAAGG TTGTCTCCAT GTTCCGCGGC 
TGCAAGACGG AGGATATGCC GCCCCACGTC TATTCACTGG CCCAGACCGC CTACAGGAGC 
CTGGTCGAGA CGCGGCGCGA CCAGAGCTTG ATATTCATGG GCCGATCTGG TTCTGGCAAA 
TCCACTAGCT TCAAGCATGC ACTCAACTAC CTGGCGTTGG CAGCTGGTGC CTATAATAAT 
TTTATAAACG CGGAGAAGGT CAATGCCCTG TGCACCATTC TGGAGGCTTT TGGAAATACG 
AAAACTTGCC TAAACTCGAA TGCCACGCGG ATGACGCAGC TGTTAAGCCT GGATTTCGAT 
CAAACCGGCC AGATTGCATC TGCCTCATTG CAAGTTCTCC TCCCAGAAAG ACAACGAGCT 
GGCCGACGAC TTGGTCATGA ACATAGCTTC CACATCATGA CGAGACTTTT GGCTGGCGCT 
GCTGGCTTGC TACAGAAGGA GCTGCACCTG GAGAACATAA CCTCTGAGGA CAGCCATCCT 
TTTATATCGT TATCCCAAAA GCTGGAAGAT CGGCATCGAG CTGCCAATGA CTTTATGCGC 
ACTGTTCAGG CCTTTGAGAC GCTGAACATT GATGCGAAGG CGGTACGTGG GATCTGGAGC 
ATTTTGGCGG CCATTTACCA TCTGGGCATT GCTGGCGTCA CAAAACTGGG CACTGGCTCC 
ACAGCCCGAA CTCAGTTTGC CAATCCCACG GCCGCCCGAA AGGCCTCGGG CTTGTTGGGC 
GTGAATCTGG AGGATCTGTC ATCAGCTGCC TTTGGTCTTA CCCAACCAAA CGCCCCCAAT 
GGCGGTCTGA GTCCATCGAA ATCGCCCACC TCGGACACTG GACACGAATG GGCCTGGGAA 
TGTCTCGAGG CGTTGGTCAT TGGTCTATAT TCGGAGGCCT TGGCTGCAGT GGTGGCATTG 
ATCAATCGTC AGATCTGCAC ATCGTCCCAC ACCATAGCCT CAATTATGCT AATTGACACG 
CCCGGCTTCC AAAATCCAGC CAGCTGTGGC CAGCAAGTGG GTGCTACTCT AGCGGATCTG 
CGTCACAACT ATCTACAGGA GCGTCTACAG ATGCTCTTCC ATCACACCAC ACTGGTGGCT 
CCCCGCGATC GCTACGCCCA GGAGCTGGTA GAAATCGAGA TGGACCTGGC ATCGGAGTGT 
CATCCGGGAC CGTTGATCTC GCTTATCGAC AAGGCACCTC AGAATCATGT AGTGCGCTCG 
TCCCAGCGAG ATTTGCGAGA GCATGATCGT CGAGGAATGT TGTGGCTACT GGATGAGGAA 
GCTATCTACC CCAACTCCAA CGATGACACA TTCCTTGAAC GTTTGTTCTC CCACTACGGT 
GACCGGGAAC ATCATAGCCT GCTGCGCAAA TGTGCAGGTC CGCGACAATT TGTACTCCAC 
CATCTGCAAG GCACCAATCC CGTTCTCTAC GCTGTCGATG GATGGGTGCG TCATAGCCGG 
GAGCACCCAG GTATTCGGAA TGCTGTATCC CTACTACAGG ACAGCAGTCG GGAGGAGATC 
AATCGCCTAT ATATCGGCTC ACTGACTCGA GGATCGGGAG CAATGGTGTT TTGTGGCTCT 
TTTGCTGGAC TGGAAGGCAC TCAGTCACTT CGTCGCGTGT CCAGTATTCG CCGCTCCTTC 
ACAACGGCCG GAGTAAAGCG AAACTCCATA ATGCTGCAGG TGAAGTTCAC AGTGGATGGA 
ATTATCGACA CTTTGCGCCG CACTGGAACT CACTTTGTGC ACTGCTATCT GCTGCAACAT 
AACGCAGGCA AACATACCAA GTACACCGCC AATGGATCGC CCAGCTCAGC TGCCGGTCAG 
GTGTCCAGCG AGGAGGAAAT GGTCAACGTG CCACTGTTAA GGAGTCAGCT ACGTGGTTCA 
CAAGTCCTAG AAGCGGCTCG CTTGCATCGC CTTGGTTTTC CCGAATCAGT GCCATTGTTG 
GAATTTGTCA GGCGTTTCGG GCTTTTGGCC GGGGATTTGG CTAGCAATAA GGATGTGAGC 
GTGGAGCAGA TATTAGCTGT CAATGAACTG GATGTGGCCA GCTATCGCAT AGGACCCAGC 
CAGATCCTCT TCCGCTCAGG AGTTCTTAGT GAACTGGAAG CCAAGCGCGA TGTCCTGCTC 
TCAGATCGCA TTATACAGCT GCAAGCATTT TGCCGCGGCT ACTTGGCACG CAAGAAGATG 
TCCCAACGAA GGGTCCAGGA ACTGGCAGTG CGTTGCATTC AACGCAATGT GAAGGCATTC 
CTTGCCGTTC GCGACTGGCC GTGGTGGCGT CTCCTGGTGC GGGTCACTCC CCTGCTCAAC 
GTTCATCGCA CCGAGGAGCA GCTGAAGACG GCTAACGAGG AGCTCCTAAT GCTGCGAGCC 
AAGCTGGAGA AGATCGAGTG TGATCGCAGT GAGGTCAAGG CAGAAAACCA AAAGCTGGAA 
GCCAAGCTAT CCGAGCTGAC GGTGGACCTG GCCGAGGAGC GATCCACGGC TCATATAGCT 
ACCGAGCGGC TGGAGGCGGA AACCGCCGAA CGCTTGAAGC TGGAGAAGGA GCTGGGCGAT 
CAAACCAACA AGGTGAAGAA CCTTCAGGAG ACGACGGAGA AGCTGGAAAT GGAGCTGATA 
TGCGCCAAGT CCGATCTGAA TGGCATCTCT GAGGACGAGG ATGCAGAGAA CGAGGATGGG 
GTCGGCGGCG GCGTCTACAA GCTTAAGTAC GAGCGGGTGG CCAGGGAGCT GGAGTTCACC 
AAAAGGCGGT TACACACGCA GCATGAGCAC GATCTGGAAC AGCTGGTCGC GCTCAAGAAG 
CATTTGGAGA TGAAGCTTTC CGATGCCTAC GAAGAAGTTG TGGAACAACG TCAGGTTGTG 
GGCCAATGGA AGCGCAAGGC ACAGAAGATG ACCAACGAGA TGAACGATCT GCGCATGCTG 
CTCGAGGAGC AAAATGCGCG CAACAATTTG CTCGAGAAGA AGCAGCGCAA GTTCGACGCC 
GAGTGCCAGT CCCTGCAGGA TGCAGTGCGT CAGGAGCGAC AGGCCAAAGA GCGCTACGGT 
CGCGAAAAGG ACGTCCTGCA GGCCGAGAAG TTCACACTGG AGCAAACGCT GGCGGACACC 
CGTCTGGATC TTGAATTCAA GGAGGAGAAA CTTGCATCGC TACAGCGCGA GCTGGAGGAG 
ATGACCTTTG GTGGCGGCAC CGAGGAGGAG TTCGCCCAAC TGCGGCGCTC CAAAAATGAA 
ACAGAGCGTC GGGCCAAGGA ACAGGAGGAA GAACTGGACG AGATGGCCGG TCAGATACAG 
CTGCTCGAAC AAGCCAAGCT TCGCCTGGAG ATGACCCTGG AAACAATGCG CAAAGAAGCG 
CGCCGTGAGT CCCAGCAGCG CGACGAAGAG CTGGAAGAAG TACGCGGTAA CGGCTACAAG 
AAGATTAAAG CCCTTGAATG CCAGCTGGAA ACTGAGCACG AGGAGCGGAC TCTGCTGCTG 
CGCGAGAAGC ACGAGCTAGA GCGACGTCTC TCCTCCATGG AGGATCGCGA TCGTGTTGAT 
CGCGATGCCG AGGAAGCGCT CAACCAAAAA CTGCGACGCG ATCTTCGCAA ATACAAGGCC 
CTGCTCAAGG ACGCCCAGAC ACAGTTGGAG CGGCTTAAGG CTGACACGCC TGGCAAGACG 
CTTATAAGAC AACTGCGTAA TCAACTGGAG GATGCCGAAT CTGCTCGTTC GCTGGCTATG 
AAAGCGCGAC AAACTGCGGA AGCCGAACTT ACTGAAGTGC AGGCCATGTT CGATGAGTCG 
CATCGAGCTA GGAATGACGC GGAGGAGCGA GCCAATGCCG CACACAGAGA TCGGGCTGAG 
CTGCAGGCCC AGATCGAGGA GAACGAGGAG GAGCTGGGCG AGCTGATGAA GAAGTACAGC 
GCCACGGTAA AGCAACTAAA TACAGAGCAA ATCAACGTAT CCGAGGCTGA GTTTAAACTC 
AATGAAATGG AAGCAGAGCG CAATAACCTC AAGGAGCAGG TGGCCGAGCT GCAGCACCGA 
CTGGACAACG TTGAGAACTT GGGGGATCCA TCTATGGCCA TGATGTCCAA GCGACTGGAG 
CTGCGCACCA AGGAATTAGA GTCTCGACTG GAACTAGAGC AGGCCACTCG GGCTCGCCTT 
GAAGTGCAGG TGAACCGTCA CAAAGAGGCT CTGGAAAAAC TGCAGAACGA GGTGACGCAG 
TCAAAGATGC GTGAGATGCA GGCCCAGGAT GTAATCAAAA AATCGCAAAA GAGTCTGCGC 
GACATGCGCG AAGAGTTCCA CGCTGTCTCC AGCCGCGAGC AGGAGTCGCT CACAAGGCGC 
AAGGACCTGG AGAAGAAGGT GGAGCAAATG GAGTCGGAGG GAGCGGCGCT TAAAAACGAT 
CTACGACTGG CTTTACAGCG AATAGCTGAT CTACAGCAGG CAATGGAGGA AGAGGGCGAG 
GAGGAGCTGA GCGAAAGTGA TGAAAGCCTC AGTTCGGTGG GCTCTATCAG TGATCTGGAA 
GATCGACTTC GGCCAGTTCA TGTAAAACGC AGCTCACAGC AGTCCTTGAA CGGCAGCATC 
GGCGGCGGAG GCGGAAGCGT TGTCAGCTCC ACCCGCACCG TGGTGTTCGA AAAGGACGAC 
AACAGCCCGA GAATAACAGT GACGTCGCCA TCGTCGCCAC ACATTCATAA GCTGGCACTG 
GCGGCCAAAG CCATACCGGC CAACAAACCG GACACAAAAC CTGCAGCGTC TACTAGGATG 
GAGTTAACAG TGCCAGCGGG CCAGCATAAT GGTCATAATG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732109.2
CDS	164..6748
Translation	MFNFMKKSAGDEDKERRKREKKLRKEAKGTSGGPGMGGVTGVTGSMSTDELLRLDEVRRSLKIRGRRKEKEKLPSGITADYSADFFAALNADANGGSNGGAVGAADPEQDRGNEEIVASVMTHIESRASNGAGVSVNYSEVTHSLLSGGLKNRFLPPIPPKPPKRGILKGSRSNMTNVHEEISFAGSAGGAGGTPEMLLRNTFQNESLYEGAARGAGSIGSNSSQHGTSFTSVESLRSDGEQTGGQQRAMTLPANARYGALPQGNSQGSHLHVLTSPSPSADSLTDTTNSSFATPPFSLSPVGESQGIDRWARVHAFEDVELPLPPVQLVKLPPPRQLVIRRQKSPRQDFGFSLRKAICLDRTESLTSPIFRPVIFAEPGAGGGATGLLPGDRLIKVNGTPVGELPREIIIEMIRNSGEAVTVEVQPVAELVELSKRCMAPSTATVEEIDHSITNGNCNTLRRSASKRFKRQSRHENGNGGGEEGGAKGVGHDLEADVADASQPERVWLVHRGGFTAAIRLPTASSGRDEENKLSLRLLHNGEQLTVDEDDVEKQNSPALDLVEDICELKYLNEASVLHCLRQRYASNLIHTKAGPTLLVVNPMAPLSLYSEKVVSMFRGCKTEDMPPHVYSLAQTAYRSLVETRRDQSLIFMGRSGSGKSTSFKHALNYLALAAGAYNNFINAEKVNALCTILEAFGNTKTCLNSNATRMTQLLSLDFDQTGQIASASLQVLLPERQRAGRRLGHEHSFHIMTRLLAGAAGLLQKELHLENITSEDSHPFISLSQKLEDRHRAANDFMRTVQAFETLNIDAKAVRGIWSILAAIYHLGIAGVTKLGTGSTARTQFANPTAARKASGLLGVNLEDLSSAAFGLTQPNAPNGGLSPSKSPTSDTGHEWAWECLEALVIGLYSEALAAVVALINRQICTSSHTIASIMLIDTPGFQNPASCGQQVGATLADLRHNYLQERLQMLFHHTTLVAPRDRYAQELVEIEMDLASECHPGPLISLIDKAPQNHVVRSSQRDLREHDRRGMLWLLDEEAIYPNSNDDTFLERLFSHYGDREHHSLLRKCAGPRQFVLHHLQGTNPVLYAVDGWVRHSREHPGIRNAVSLLQDSSREEINRLYIGSLTRGSGAMVFCGSFAGLEGTQSLRRVSSIRRSFTTAGVKRNSIMLQVKFTVDGIIDTLRRTGTHFVHCYLLQHNAGKHTKYTANGSPSSAAGQVSSEEEMVNVPLLRSQLRGSQVLEAARLHRLGFPESVPLLEFVRRFGLLAGDLASNKDVSVEQILAVNELDVASYRIGPSQILFRSGVLSELEAKRDVLLSDRIIQLQAFCRGYLARKKMSQRRVQELAVRCIQRNVKAFLAVRDWPWWRLLVRVTPLLNVHRTEEQLKTANEELLMLRAKLEKIECDRSEVKAENQKLEAKLSELTVDLAEERSTAHIATERLEAETAERLKLEKELGDQTNKVKNLQETTEKLEMELICAKSDLNGISEDEDAENEDGVGGGVYKLKYERVARELEFTKRRLHTQHEHDLEQLVALKKHLEMKLSDAYEEVVEQRQVVGQWKRKAQKMTNEMNDLRMLLEEQNARNNLLEKKQRKFDAECQSLQDAVRQERQAKERYGREKDVLQAEKFTLEQTLADTRLDLEFKEEKLASLQRELEEMTFGGGTEEEFAQLRRSKNETERRAKEQEEELDEMAGQIQLLEQAKLRLEMTLETMRKEARRESQQRDEELEEVRGNGYKKIKALECQLETEHEERTLLLREKHELERRLSSMEDRDRVDRDAEEALNQKLRRDLRKYKALLKDAQTQLERLKADTPGKTLIRQLRNQLEDAESARSLAMKARQTAEAELTEVQAMFDESHRARNDAEERANAAHRDRAELQAQIEENEEELGELMKKYSATVKQLNTEQINVSEAEFKLNEMEAERNNLKEQVAELQHRLDNVENLGDPSMAMMSKRLELRTKELESRLELEQATRARLEVQVNRHKEALEKLQNEVTQSKMREMQAQDVIKKSQKSLRDMREEFHAVSSREQESLTRRKDLEKKVEQMESEGAALKNDLRLALQRIADLQQAMEEEGEEELSESDESLSSVGSISDLEDRLRPVHVKRSSQQSLNGSIGGGGGSVVSSTRTVVFEKDDNSPRITVTSPSSPHIHKLALAAKAIPANKPDTKPAASTRMELTVPAGQHNGHNA

Target ORF information:

RefSeq Version	NM_169701.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain-like, transcript variant B (Mhcl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169701.3

ORF Insert Sequence:

ATGTTCAACT TTATGAAGAA GAGCGCTGGG GATGAGGACA AGGAGCGTCG CAAGCGCGAA 
AAAAAACTGC GCAAAGAGGC TAAGGGCACC TCTGGTGGCC CGGGAATGGG AGGCGTGACT 
GGCGTCACCG GCAGCATGAG CACCGACGAG CTGCTCCGCC TGGACGAGGT GCGCCGATCG 
CTTAAAATCC GAGGGCGACG CAAGGAGAAG GAAAAGTTGC CGAGCGGAAT TACGGCGGAC 
TATAGCGCAG ACTTCTTTGC CGCCCTCAAT GCAGATGCAA ACGGCGGAAG CAACGGAGGA 
GCAGTGGGAG CTGCAGATCC CGAGCAGGAT CGTGGCAATG AGGAGATTGT AGCATCTGTG 
ATGACGCACA TCGAGAGCCG AGCGTCCAAT GGCGCCGGCG TGAGTGTCAA CTACAGCGAG 
GTCACACATT CTCTCCTCTC TGGAGGGCTC AAGAATCGCT TTCTTCCACC TATACCCCCA 
AAACCACCCA AGCGTGGCAT TCTGAAAGGC TCCCGCAGTA ATATGACCAA TGTCCACGAG 
GAGATTAGCT TTGCGGGTAG TGCCGGAGGA GCAGGTGGCA CGCCAGAGAT GCTATTGCGC 
AACACCTTTC AGAATGAATC CCTTTACGAG GGAGCTGCCA GGGGAGCTGG TTCGATCGGC 
TCGAACTCCA GTCAGCATGG AACCTCGTTC ACTTCCGTGG AGAGCCTGCG CAGCGATGGA 
GAGCAGACAG GAGGTCAACA ACGGGCCATG ACCCTGCCGG CCAACGCCCG CTATGGAGCT 
TTACCCCAGG GCAACAGTCA AGGATCGCAC CTCCATGTGC TCACCTCGCC GTCGCCCAGC 
GCCGACAGTC TCACAGATAC CACAAACTCC TCTTTCGCCA CACCCCCATT TTCGCTGAGT 
CCCGTCGGCG AGTCCCAAGG CATCGACCGT TGGGCCCGCG TCCATGCCTT CGAGGATGTG 
GAGCTGCCGC TGCCGCCAGT GCAGCTAGTA AAACTTCCCC CACCAAGGCA GCTAGTGATC 
AGGCGACAGA AGTCTCCGCG CCAGGACTTC GGCTTTAGCC TGCGCAAGGC CATCTGTTTG 
GACAGAACAG AGTCCCTGAC TTCGCCCATT TTCAGGCCGG TTATATTCGC CGAGCCAGGT 
GCTGGTGGCG GAGCCACAGG TCTTTTGCCT GGCGATCGCC TAATCAAGGT CAACGGCACG 
CCGGTTGGGG AACTGCCGCG GGAGATCATA ATTGAGATGA TACGGAACAG CGGAGAGGCT 
GTCACCGTGG AGGTCCAACC AGTCGCTGAG TTGGTGGAGC TGAGCAAGCG ATGCATGGCC 
CCCAGCACAG CCACCGTGGA GGAGATCGAT CATTCCATTA CCAACGGCAA CTGCAATACC 
CTGCGACGCA GTGCCAGCAA GCGCTTCAAG CGTCAGAGCC GCCATGAGAA TGGCAATGGC 
GGAGGAGAAG AGGGAGGAGC CAAAGGAGTG GGCCACGATC TGGAGGCGGA TGTTGCGGAT 
GCCAGTCAGC CGGAGCGCGT ATGGCTGGTG CACCGCGGTG GTTTCACTGC CGCCATCCGT 
CTGCCGACTG CGTCTTCTGG CCGGGATGAG GAGAACAAGC TAAGCCTGCG CCTCCTGCAT 
AATGGGGAGC AGCTGACCGT CGACGAGGAC GATGTCGAGA AGCAGAACAG CCCCGCCCTG 
GACCTTGTCG AGGACATCTG CGAGCTGAAG TATCTGAACG AGGCGTCCGT GCTCCATTGC 
CTGCGTCAGC GATACGCCAG CAATCTCATC CACACCAAGG CGGGGCCCAC GCTGCTCGTG 
GTCAATCCCA TGGCACCGCT GTCCCTGTAC TCCGAGAAGG TTGTCTCCAT GTTCCGCGGC 
TGCAAGACGG AGGATATGCC GCCCCACGTC TATTCACTGG CCCAGACCGC CTACAGGAGC 
CTGGTCGAGA CGCGGCGCGA CCAGAGCTTG ATATTCATGG GCCGATCTGG TTCTGGCAAA 
TCCACTAGCT TCAAGCATGC ACTCAACTAC CTGGCGTTGG CAGCTGGTGC CTATAATAAT 
TTTATAAACG CGGAGAAGGT CAATGCCCTG TGCACCATTC TGGAGGCTTT TGGAAATACG 
AAAACTTGCC TAAACTCGAA TGCCACGCGG ATGACGCAGC TGTTAAGCCT GGATTTCGAT 
CAAACCGGCC AGATTGCATC TGCCTCATTG CAAGTTCTCC TCCCAGAAAG ACAACGAGCT 
GGCCGACGAC TTGGTCATGA ACATAGCTTC CACATCATGA CGAGACTTTT GGCTGGCGCT 
GCTGGCTTGC TACAGAAGGA GCTGCACCTG GAGAACATAA CCTCTGAGGA CAGCCATCCT 
TTTATATCGT TATCCCAAAA GCTGGAAGAT CGGCATCGAG CTGCCAATGA CTTTATGCGC 
ACTGTTCAGG CCTTTGAGAC GCTGAACATT GATGCGAAGG CGGTACGTGG GATCTGGAGC 
ATTTTGGCGG CCATTTACCA TCTGGGCATT GCTGGCGTCA CAAAACTGGG CACTGGCTCC 
ACAGCCCGAA CTCAGTTTGC CAATCCCACG GCCGCCCGAA AGGCCTCGGG CTTGTTGGGC 
GTGAATCTGG AGGATCTGTC ATCAGCTGCC TTTGGTCTTA CCCAACCAAA CGCCCCCAAT 
GGCGGTCTGA GTCCATCGAA ATCGCCCACC TCGGACACTG GACACGAATG GGCCTGGGAA 
TGTCTCGAGG CGTTGGTCAT TGGTCTATAT TCGGAGGCCT TGGCTGCAGT GGTGGCATTG 
ATCAATCGTC AGATCTGCAC ATCGTCCCAC ACCATAGCCT CAATTATGCT AATTGACACG 
CCCGGCTTCC AAAATCCAGC CAGCTGTGGC CAGCAAGTGG GTGCTACTCT AGCGGATCTG 
CGTCACAACT ATCTACAGGA GCGTCTACAG ATGCTCTTCC ATCACACCAC ACTGGTGGCT 
CCCCGCGATC GCTACGCCCA GGAGCTGGTA GAAATCGAGA TGGACCTGGC ATCGGAGTGT 
CATCCGGGAC CGTTGATCTC GCTTATCGAC AAGGCACCTC AGAATCATGT AGTGCGCTCG 
TCCCAGCGAG ATTTGCGAGA GCATGATCGT CGAGGAATGT TGTGGCTACT GGATGAGGAA 
GCTATCTACC CCAACTCCAA CGATGACACA TTCCTTGAAC GTTTGTTCTC CCACTACGGT 
GACCGGGAAC ATCATAGCCT GCTGCGCAAA TGTGCAGGTC CGCGACAATT TGTACTCCAC 
CATCTGCAAG GCACCAATCC CGTTCTCTAC GCTGTCGATG GATGGGTGCG TCATAGCCGG 
GAGCACCCAG GTATTCGGAA TGCTGTATCC CTACTACAGG ACAGCAGTCG GGAGGAGATC 
AATCGCCTAT ATATCGGCTC ACTGACTCGA GGATCGGGAG CAATGGTGTT TTGTGGCTCT 
TTTGCTGGAC TGGAAGGCAC TCAGTCACTT CGTCGCGTGT CCAGTATTCG CCGCTCCTTC 
ACAACGGCCG GAGTAAAGCG AAACTCCATA ATGCTGCAGG TGAAGTTCAC AGTGGATGGA 
ATTATCGACA CTTTGCGCCG CACTGGAACT CACTTTGTGC ACTGCTATCT GCTGCAACAT 
AACGCAGGCA AACATACCAA GTACACCGCC AATGGATCGC CCAGCTCAGC TGCCGGTCAG 
GTGTCCAGCG AGGAGGAAAT GGTCAACGTG CCACTGTTAA GGAGTCAGCT ACGTGGTTCA 
CAAGTCCTAG AAGCGGCTCG CTTGCATCGC CTTGGTTTTC CCGAATCAGT GCCATTGTTG 
GAATTTGTCA GGCGTTTCGG GCTTTTGGCC GGGGATTTGG CTAGCAATAA GGATGTGAGC 
GTGGAGCAGA TATTAGCTGT CAATGAACTG GATGTGGCCA GCTATCGCAT AGGACCCAGC 
CAGATCCTCT TCCGCTCAGG AGTTCTTAGT GAACTGGAAG CCAAGCGCGA TGTCCTGCTC 
TCAGATCGCA TTATACAGCT GCAAGCATTT TGCCGCGGCT ACTTGGCACG CAAGAAGATG 
TCCCAACGAA GGGTCCAGGA ACTGGCAGTG CGTTGCATTC AACGCAATGT GAAGGCATTC 
CTTGCCGTTC GCGACTGGCC GTGGTGGCGT CTCCTGGTGC GGGTCACTCC CCTGCTCAAC 
GTTCATCGCA CCGAGGAGCA GCTGAAGACG GCTAACGAGG AGCTCCTAAT GCTGCGAGCC 
AAGCTGGAGA AGATCGAGTG TGATCGCAGT GAGGTCAAGG CAGAAAACCA AAAGCTGGAA 
GCCAAGCTAT CCGAGCTGAC GGTGGACCTG GCCGAGGAGC GATCCACGGC TCATATAGCT 
ACCGAGCGGC TGGAGGCGGA AACCGCCGAA CGCTTGAAGC TGGAGAAGGA GCTGGGCGAT 
CAAACCAACA AGGTGAAGAA CCTTCAGGAG ACGACGGAGA AGCTGGAAAT GGAGCTGATA 
TGCGCCAAGT CCGATCTGAA TGGCATCTCT GAGGACGAGG ATGCAGAGAA CGAGGATGGG 
GTCGGCGGCG GCGTCTACAA GCTTAAGTAC GAGCGGGTGG CCAGGGAGCT GGAGTTCACC 
AAAAGGCGGT TACACACGCA GCATGAGCAC GATCTGGAAC AGCTGGTCGC GCTCAAGAAG 
CATTTGGAGA TGAAGCTTTC CGATGCCTAC GAAGAAGTTG TGGAACAACG TCAGGTTGTG 
GGCCAATGGA AGCGCAAGGC ACAGAAGATG ACCAACGAGA TGAACGATCT GCGCATGCTG 
CTCGAGGAGC AAAATGCGCG CAACAATTTG CTCGAGAAGA AGCAGCGCAA GTTCGACGCC 
GAGTGCCAGT CCCTGCAGGA TGCAGTGCGT CAGGAGCGAC AGGCCAAAGA GCGCTACGGT 
CGCGAAAAGG ACGTCCTGCA GGCCGAGAAG TTCACACTGG AGCAAACGCT GGCGGACACC 
CGTCTGGATC TTGAATTCAA GGAGGAGAAA CTTGCATCGC TACAGCGCGA GCTGGAGGAG 
ATGACCTTTG GTGGCGGCAC CGAGGAGGAG TTCGCCCAAC TGCGGCGCTC CAAAAATGAA 
ACAGAGCGTC GGGCCAAGGA ACAGGAGGAA GAACTGGACG AGATGGCCGG TCAGATACAG 
CTGCTCGAAC AAGCCAAGCT TCGCCTGGAG ATGACCCTGG AAACAATGCG CAAAGAAGCG 
CGCCGTGAGT CCCAGCAGCG CGACGAAGAG CTGGAAGAAG TACGCGGTAA CGGCTACAAG 
AAGATTAAAG CCCTTGAATG CCAGCTGGAA ACTGAGCACG AGGAGCGGAC TCTGCTGCTG 
CGCGAGAAGC ACGAGCTAGA GCGACGTCTC TCCTCCATGG AGGATCGCGA TCGTGTTGAT 
CGCGATGCCG AGGAAGCGCT CAACCAAAAA CTGCGACGCG ATCTTCGCAA ATACAAGGCC 
CTGCTCAAGG ACGCCCAGAC ACAGTTGGAG CGGCTTAAGG CTGACACGCC TGGCAAGACG 
CTTATAAGAC AACTGCGTAA TCAACTGGAG GATGCCGAAT CTGCTCGTTC GCTGGCTATG 
AAAGCGCGAC AAACTGCGGA AGCCGAACTT ACTGAAGTGC AGGCCATGTT CGATGAGTCG 
CATCGAGCTA GGAATGACGC GGAGGAGCGA GCCAATGCCG CACACAGAGA TCGGGCTGAG 
CTGCAGGCCC AGATCGAGGA GAACGAGGAG GAGCTGGGCG AGCTGATGAA GAAGTACAGC 
GCCACGGTAA AGCAACTAAA TACAGAGCAA ATCAACGTAT CCGAGGCTGA GTTTAAACTC 
AATGAAATGG AAGCAGAGCG CAATAACCTC AAGGAGCAGG TGGCCGAGCT GCAGCACCGA 
CTGGACAACG TTGAGAACTT GGGGGATCCA TCTATGGCCA TGATGTCCAA GCGACTGGAG 
CTGCGCACCA AGGAATTAGA GTCTCGACTG GAACTAGAGC AGGCCACTCG GGCTCGCCTT 
GAAGTGCAGG TGAACCGTCA CAAAGAGGCT CTGGAAAAAC TGCAGAACGA GGTGACGCAG 
TCAAAGATGC GTGAGATGCA GGCCCAGGAT GTAATCAAAA AATCGCAAAA GAGTCTGCGC 
GACATGCGCG AAGAGTTCCA CGCTGTCTCC AGCCGCGAGC AGGAGTCGCT CACAAGGCGC 
AAGGACCTGG AGAAGAAGGT GGAGCAAATG GAGTCGGAGG GAGCGGCGCT TAAAAACGAT 
CTACGACTGG CTTTACAGCG AATAGCTGAT CTACAGCAGG CAATGGAGGA AGAGGGCGAG 
GAGGAGCTGA GCGAAAGTGA TGAAAGCCTC AGTTCGGTGG GCTCTATCAG TGATCTGGAA 
GATCGACTTC GGCCAGTTCA TGTAAAACGC AGCTCACAGC AGTCCTTGAA CGGCAGCATC 
GGCGGCGGAG GCGGAAGCGT TGTCAGCTCC ACCCGCACCG TGGTGTTCGA AAAGGACGAC 
AACAGCCCGA GAATAACAGT GACGTCGCCA TCGTCGCCAC ACATTCATAA GCTGGCACTG 
GCGGCCAAAG CCATACCGGC CAACAAACCG GACACAAAAC CTGCAGCGTC TACTAGGATG 
GAGTTAACAG TGCCAGCGGG CCAGCATAAT GGTCATAATG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mhcl ( NM_001043250.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036715.1 Drosophila melanogaster myosin heavy chain-like, transcript variant G (Mhcl), mRNA.

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CloneID	OFa05084
Clone ID Related Accession (Same CDS sequence)	NM_001043250.2
Accession Version	NM_001043250.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	myosin heavy chain-like, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 31, 2011 this sequence version replaced NM_001043250.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCAACT TTATGAAGAA GAGCGCTGGG GATGAGGACA AGGAGCGTCG CAAGCGCGAA 
AAAAAACTGC GCAAAGAGGC TAAGGGCACC TCTGGTGGCC CGGGAATGGG AGGCGTGACT 
GGCGTCACCG GCAGCATGAG CACCGACGAG CTGCTCCGCC TGGACGAGGT GCGCCGATCG 
CTTAAAATCC GAGGGCGACG CAAGGAGAAG GAAAAGTTGC CGAGCGGAAT TACGGCGGAC 
TATAGCGCAG ACTTCTTTGC CGCCCTCAAT GCAGATGCAA ACGGCGGAAG CAACGGAGGA 
GCAGTGGGAG CTGCAGATCC CGAGCAGGAT CGTGGCAATG AGGAGATTGT AGCATCTGTG 
ATGACGCACA TCGAGAGCCG AGCGTCCAAT GGCGCCGGCG TGAGTGTCAA CTACAGCGAG 
GTCACACATT CTCTCCTCTC TGGAGGGCTC AAGAATCGCT TTCTTCCACC TATACCCCCA 
AAACCACCCA AGCGTGGCAT TCTGAAAGGC TCCCGCAGTA ATATGACCAA TGTCCACGAG 
GAGATTAGCT TTGCGGGTAG TGCCGGAGGA GCAGGTGGCA CGCCAGAGAT GCTATTGCGC 
AACACCTTTC AGAATGAATC CCTTTACGAG GGAGCTGCCA GGGGAGCTGG TTCGATCGGC 
TCGAACTCCA GTCAGCATGG AACCTCGTTC ACTTCCGTGG AGAGCCTGCG CAGCGATGGA 
GAGCAGACAG GAGGTCAACA ACGGGCCATG ACCCTGCCGG CCAACGCCCG CTATGGAGCT 
TTACCCCAGG GCAACAGTCA AGGATCGCAC CTCCATGTGC TCACCTCGCC GTCGCCCAGC 
GCCGACAGTC TCACAGATAC CACAAACTCC TCTTTCGCCA CACCCCCATT TTCGCTGAGT 
CCCGTCGGCG AGTCCCAAGG CATCGACCGT TGGGCCCGCG TCCATGCCTT CGAGGATGTG 
GAGCTGCCGC TGCCGCCAGT GCAGCTAGTA AAACTTCCCC CACCAAGGCA GCTAGTGATC 
AGGCGACAGA AGTCTCCGCG CCAGGACTTC GGCTTTAGCC TGCGCAAGGC CATCTGTTTG 
GACAGAACAG AGTCCCTGAC TTCGCCCATT TTCAGGCCGG TTATATTCGC CGAGCCAGGT 
GCTGGTGGCG GAGCCACAGG TCTTTTGCCT GGCGATCGCC TAATCAAGGT CAACGGCACG 
CCGGTTGGGG AACTGCCGCG GGAGATCATA ATTGAGATGA TACGGAACAG CGGAGAGGCT 
GTCACCGTGG AGGTCCAACC AGTCGCTGAG TTGGTGGAGC TGAGCAAGCG ATGCATGGCC 
CCCAGCACAG CCACCGTGGA GGAGATCGAT CATTCCATTA CCAACGGCAA CTGCAATACC 
CTGCGACGCA GTGCCAGCAA GCGCTTCAAG CGTCAGAGCC GCCATGAGAA TGGCAATGGC 
GGAGGAGAAG AGGGAGGAGC CAAAGGAGTG GGCCACGATC TGGAGGCGGA TGTTGCGGAT 
GCCAGTCAGC CGGAGCGCGT ATGGCTGGTG CACCGCGGTG GTTTCACTGC CGCCATCCGT 
CTGCCGACTG CGTCTTCTGG CCGGGATGAG GAGAACAAGC TAAGCCTGCG CCTCCTGCAT 
AATGGGGAGC AGCTGACCGT CGACGAGGAC GATGTCGAGA AGCAGAACAG CCCCGCCCTG 
GACCTTGTCG AGGACATCTG CGAGCTGAAG TATCTGAACG AGGCGTCCGT GCTCCATTGC 
CTGCGTCAGC GATACGCCAG CAATCTCATC CACACCAAGG CGGGGCCCAC GCTGCTCGTG 
GTCAATCCCA TGGCACCGCT GTCCCTGTAC TCCGAGAAGG TTGTCTCCAT GTTCCGCGGC 
TGCAAGACGG AGGATATGCC GCCCCACGTC TATTCACTGG CCCAGACCGC CTACAGGAGC 
CTGGTCGAGA CGCGGCGCGA CCAGAGCTTG ATATTCATGG GCCGATCTGG TTCTGGCAAA 
TCCACTAGCT TCAAGCATGC ACTCAACTAC CTGGCGTTGG CAGCTGGTGC CTATAATAAT 
TTTATAAACG CGGAGAAGGT CAATGCCCTG TGCACCATTC TGGAGGCTTT TGGAAATACG 
AAAACTTGCC TAAACTCGAA TGCCACGCGG ATGACGCAGC TGTTAAGCCT GGATTTCGAT 
CAAACCGGCC AGATTGCATC TGCCTCATTG CAAGTTCTCC TCCCAGAAAG ACAACGAGCT 
GGCCGACGAC TTGGTCATGA ACATAGCTTC CACATCATGA CGAGACTTTT GGCTGGCGCT 
GCTGGCTTGC TACAGAAGGA GCTGCACCTG GAGAACATAA CCTCTGAGGA CAGCCATCCT 
TTTATATCGT TATCCCAAAA GCTGGAAGAT CGGCATCGAG CTGCCAATGA CTTTATGCGC 
ACTGTTCAGG CCTTTGAGAC GCTGAACATT GATGCGAAGG CGGTACGTGG GATCTGGAGC 
ATTTTGGCGG CCATTTACCA TCTGGGCATT GCTGGCGTCA CAAAACTGGG CACTGGCTCC 
ACAGCCCGAA CTCAGTTTGC CAATCCCACG GCCGCCCGAA AGGCCTCGGG CTTGTTGGGC 
GTGAATCTGG AGGATCTGTC ATCAGCTGCC TTTGGTCTTA CCCAACCAAA CGCCCCCAAT 
GGCGGTCTGA GTCCATCGAA ATCGCCCACC TCGGACACTG GACACGAATG GGCCTGGGAA 
TGTCTCGAGG CGTTGGTCAT TGGTCTATAT TCGGAGGCCT TGGCTGCAGT GGTGGCATTG 
ATCAATCGTC AGATCTGCAC ATCGTCCCAC ACCATAGCCT CAATTATGCT AATTGACACG 
CCCGGCTTCC AAAATCCAGC CAGCTGTGGC CAGCAAGTGG GTGCTACTCT AGCGGATCTG 
CGTCACAACT ATCTACAGGA GCGTCTACAG ATGCTCTTCC ATCACACCAC ACTGGTGGCT 
CCCCGCGATC GCTACGCCCA GGAGCTGGTA GAAATCGAGA TGGACCTGGC ATCGGAGTGT 
CATCCGGGAC CGTTGATCTC GCTTATCGAC AAGGCACCTC AGAATCATGT AGTGCGCTCG 
TCCCAGCGAG ATTTGCGAGA GCATGATCGT CGAGGAATGT TGTGGCTACT GGATGAGGAA 
GCTATCTACC CCAACTCCAA CGATGACACA TTCCTTGAAC GTTTGTTCTC CCACTACGGT 
GACCGGGAAC ATCATAGCCT GCTGCGCAAA TGTGCAGGTC CGCGACAATT TGTACTCCAC 
CATCTGCAAG GCACCAATCC CGTTCTCTAC GCTGTCGATG GATGGGTGCG TCATAGCCGG 
GAGCACCCAG GTATTCGGAA TGCTGTATCC CTACTACAGG ACAGCAGTCG GGAGGAGATC 
AATCGCCTAT ATATCGGCTC ACTGACTCGA GGATCGGGAG CAATGGTGTT TTGTGGCTCT 
TTTGCTGGAC TGGAAGGCAC TCAGTCACTT CGTCGCGTGT CCAGTATTCG CCGCTCCTTC 
ACAACGGCCG GAGTAAAGCG AAACTCCATA ATGCTGCAGG TGAAGTTCAC AGTGGATGGA 
ATTATCGACA CTTTGCGCCG CACTGGAACT CACTTTGTGC ACTGCTATCT GCTGCAACAT 
AACGCAGGCA AACATACCAA GTACACCGCC AATGGATCGC CCAGCTCAGC TGCCGGTCAG 
GTGTCCAGCG AGGAGGAAAT GGTCAACGTG CCACTGTTAA GGAGTCAGCT ACGTGGTTCA 
CAAGTCCTAG AAGCGGCTCG CTTGCATCGC CTTGGTTTTC CCGAATCAGT GCCATTGTTG 
GAATTTGTCA GGCGTTTCGG GCTTTTGGCC GGGGATTTGG CTAGCAATAA GGATGTGAGC 
GTGGAGCAGA TATTAGCTGT CAATGAACTG GATGTGGCCA GCTATCGCAT AGGACCCAGC 
CAGATCCTCT TCCGCTCAGG AGTTCTTAGT GAACTGGAAG CCAAGCGCGA TGTCCTGCTC 
TCAGATCGCA TTATACAGCT GCAAGCATTT TGCCGCGGCT ACTTGGCACG CAAGAAGATG 
TCCCAACGAA GGGTCCAGGA ACTGGCAGTG CGTTGCATTC AACGCAATGT GAAGGCATTC 
CTTGCCGTTC GCGACTGGCC GTGGTGGCGT CTCCTGGTGC GGGTCACTCC CCTGCTCAAC 
GTTCATCGCA CCGAGGAGCA GCTGAAGACG GCTAACGAGG AGCTCCTAAT GCTGCGAGCC 
AAGCTGGAGA AGATCGAGTG TGATCGCAGT GAGGTCAAGG CAGAAAACCA AAAGCTGGAA 
GCCAAGCTAT CCGAGCTGAC GGTGGACCTG GCCGAGGAGC GATCCACGGC TCATATAGCT 
ACCGAGCGGC TGGAGGCGGA AACCGCCGAA CGCTTGAAGC TGGAGAAGGA GCTGGGCGAT 
CAAACCAACA AGGTGAAGAA CCTTCAGGAG ACGACGGAGA AGCTGGAAAT GGAGCTGATA 
TGCGCCAAGT CCGATCTGAA TGGCATCTCT GAGGACGAGG ATGCAGAGAA CGAGGATGGG 
GTCGGCGGCG GCGTCTACAA GCTTAAGTAC GAGCGGGTGG CCAGGGAGCT GGAGTTCACC 
AAAAGGCGGT TACACACGCA GCATGAGCAC GATCTGGAAC AGCTGGTCGC GCTCAAGAAG 
CATTTGGAGA TGAAGCTTTC CGATGCCTAC GAAGAAGTTG TGGAACAACG TCAGGTTGTG 
GGCCAATGGA AGCGCAAGGC ACAGAAGATG ACCAACGAGA TGAACGATCT GCGCATGCTG 
CTCGAGGAGC AAAATGCGCG CAACAATTTG CTCGAGAAGA AGCAGCGCAA GTTCGACGCC 
GAGTGCCAGT CCCTGCAGGA TGCAGTGCGT CAGGAGCGAC AGGCCAAAGA GCGCTACGGT 
CGCGAAAAGG ACGTCCTGCA GGCCGAGAAG TTCACACTGG AGCAAACGCT GGCGGACACC 
CGTCTGGATC TTGAATTCAA GGAGGAGAAA CTTGCATCGC TACAGCGCGA GCTGGAGGAG 
ATGACCTTTG GTGGCGGCAC CGAGGAGGAG TTCGCCCAAC TGCGGCGCTC CAAAAATGAA 
ACAGAGCGTC GGGCCAAGGA ACAGGAGGAA GAACTGGACG AGATGGCCGG TCAGATACAG 
CTGCTCGAAC AAGCCAAGCT TCGCCTGGAG ATGACCCTGG AAACAATGCG CAAAGAAGCG 
CGCCGTGAGT CCCAGCAGCG CGACGAAGAG CTGGAAGAAG TACGCGGTAA CGGCTACAAG 
AAGATTAAAG CCCTTGAATG CCAGCTGGAA ACTGAGCACG AGGAGCGGAC TCTGCTGCTG 
CGCGAGAAGC ACGAGCTAGA GCGACGTCTC TCCTCCATGG AGGATCGCGA TCGTGTTGAT 
CGCGATGCCG AGGAAGCGCT CAACCAAAAA CTGCGACGCG ATCTTCGCAA ATACAAGGCC 
CTGCTCAAGG ACGCCCAGAC ACAGTTGGAG CGGCTTAAGG CTGACACGCC TGGCAAGACG 
CTTATAAGAC AACTGCGTAA TCAACTGGAG GATGCCGAAT CTGCTCGTTC GCTGGCTATG 
AAAGCGCGAC AAACTGCGGA AGCCGAACTT ACTGAAGTGC AGGCCATGTT CGATGAGTCG 
CATCGAGCTA GGAATGACGC GGAGGAGCGA GCCAATGCCG CACACAGAGA TCGGGCTGAG 
CTGCAGGCCC AGATCGAGGA GAACGAGGAG GAGCTGGGCG AGCTGATGAA GAAGTACAGC 
GCCACGGTAA AGCAACTAAA TACAGAGCAA ATCAACGTAT CCGAGGCTGA GTTTAAACTC 
AATGAAATGG AAGCAGAGCG CAATAACCTC AAGGAGCAGG TGGCCGAGCT GCAGCACCGA 
CTGGACAACG TTGAGAACTT GGGGGATCCA TCTATGGCCA TGATGTCCAA GCGACTGGAG 
CTGCGCACCA AGGAATTAGA GTCTCGACTG GAACTAGAGC AGGCCACTCG GGCTCGCCTT 
GAAGTGCAGG TGAACCGTCA CAAAGAGGCT CTGGAAAAAC TGCAGAACGA GGTGACGCAG 
TCAAAGATGC GTGAGATGCA GGCCCAGGAT GTAATCAAAA AATCGCAAAA GAGTCTGCGC 
GACATGCGCG AAGAGTTCCA CGCTGTCTCC AGCCGCGAGC AGGAGTCGCT CACAAGGCGC 
AAGGACCTGG AGAAGAAGGT GGAGCAAATG GAGTCGGAGG GAGCGGCGCT TAAAAACGAT 
CTACGACTGG CTTTACAGCG AATAGCTGAT CTACAGCAGG CAATGGAGGA AGAGGGCGAG 
GAGGAGCTGA GCGAAAGTGA TGAAAGCCTC AGTTCGGTGG GCTCTATCAG TGATCTGGAA 
GATCGACTTC GGCCAGTTCA TGTAAAACGC AGCTCACAGC AGTCCTTGAA CGGCAGCATC 
GGCGGCGGAG GCGGAAGCGT TGTCAGCTCC ACCCGCACCG TGGTGTTCGA AAAGGACGAC 
AACAGCCCGA GGTACGCTGA TAACGAAGTC AACTCGAACA GCAGAAAGCA GCACAAGCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036715.1
CDS	164..6646
Translation	MFNFMKKSAGDEDKERRKREKKLRKEAKGTSGGPGMGGVTGVTGSMSTDELLRLDEVRRSLKIRGRRKEKEKLPSGITADYSADFFAALNADANGGSNGGAVGAADPEQDRGNEEIVASVMTHIESRASNGAGVSVNYSEVTHSLLSGGLKNRFLPPIPPKPPKRGILKGSRSNMTNVHEEISFAGSAGGAGGTPEMLLRNTFQNESLYEGAARGAGSIGSNSSQHGTSFTSVESLRSDGEQTGGQQRAMTLPANARYGALPQGNSQGSHLHVLTSPSPSADSLTDTTNSSFATPPFSLSPVGESQGIDRWARVHAFEDVELPLPPVQLVKLPPPRQLVIRRQKSPRQDFGFSLRKAICLDRTESLTSPIFRPVIFAEPGAGGGATGLLPGDRLIKVNGTPVGELPREIIIEMIRNSGEAVTVEVQPVAELVELSKRCMAPSTATVEEIDHSITNGNCNTLRRSASKRFKRQSRHENGNGGGEEGGAKGVGHDLEADVADASQPERVWLVHRGGFTAAIRLPTASSGRDEENKLSLRLLHNGEQLTVDEDDVEKQNSPALDLVEDICELKYLNEASVLHCLRQRYASNLIHTKAGPTLLVVNPMAPLSLYSEKVVSMFRGCKTEDMPPHVYSLAQTAYRSLVETRRDQSLIFMGRSGSGKSTSFKHALNYLALAAGAYNNFINAEKVNALCTILEAFGNTKTCLNSNATRMTQLLSLDFDQTGQIASASLQVLLPERQRAGRRLGHEHSFHIMTRLLAGAAGLLQKELHLENITSEDSHPFISLSQKLEDRHRAANDFMRTVQAFETLNIDAKAVRGIWSILAAIYHLGIAGVTKLGTGSTARTQFANPTAARKASGLLGVNLEDLSSAAFGLTQPNAPNGGLSPSKSPTSDTGHEWAWECLEALVIGLYSEALAAVVALINRQICTSSHTIASIMLIDTPGFQNPASCGQQVGATLADLRHNYLQERLQMLFHHTTLVAPRDRYAQELVEIEMDLASECHPGPLISLIDKAPQNHVVRSSQRDLREHDRRGMLWLLDEEAIYPNSNDDTFLERLFSHYGDREHHSLLRKCAGPRQFVLHHLQGTNPVLYAVDGWVRHSREHPGIRNAVSLLQDSSREEINRLYIGSLTRGSGAMVFCGSFAGLEGTQSLRRVSSIRRSFTTAGVKRNSIMLQVKFTVDGIIDTLRRTGTHFVHCYLLQHNAGKHTKYTANGSPSSAAGQVSSEEEMVNVPLLRSQLRGSQVLEAARLHRLGFPESVPLLEFVRRFGLLAGDLASNKDVSVEQILAVNELDVASYRIGPSQILFRSGVLSELEAKRDVLLSDRIIQLQAFCRGYLARKKMSQRRVQELAVRCIQRNVKAFLAVRDWPWWRLLVRVTPLLNVHRTEEQLKTANEELLMLRAKLEKIECDRSEVKAENQKLEAKLSELTVDLAEERSTAHIATERLEAETAERLKLEKELGDQTNKVKNLQETTEKLEMELICAKSDLNGISEDEDAENEDGVGGGVYKLKYERVARELEFTKRRLHTQHEHDLEQLVALKKHLEMKLSDAYEEVVEQRQVVGQWKRKAQKMTNEMNDLRMLLEEQNARNNLLEKKQRKFDAECQSLQDAVRQERQAKERYGREKDVLQAEKFTLEQTLADTRLDLEFKEEKLASLQRELEEMTFGGGTEEEFAQLRRSKNETERRAKEQEEELDEMAGQIQLLEQAKLRLEMTLETMRKEARRESQQRDEELEEVRGNGYKKIKALECQLETEHEERTLLLREKHELERRLSSMEDRDRVDRDAEEALNQKLRRDLRKYKALLKDAQTQLERLKADTPGKTLIRQLRNQLEDAESARSLAMKARQTAEAELTEVQAMFDESHRARNDAEERANAAHRDRAELQAQIEENEEELGELMKKYSATVKQLNTEQINVSEAEFKLNEMEAERNNLKEQVAELQHRLDNVENLGDPSMAMMSKRLELRTKELESRLELEQATRARLEVQVNRHKEALEKLQNEVTQSKMREMQAQDVIKKSQKSLRDMREEFHAVSSREQESLTRRKDLEKKVEQMESEGAALKNDLRLALQRIADLQQAMEEEGEEELSESDESLSSVGSISDLEDRLRPVHVKRSSQQSLNGSIGGGGGSVVSSTRTVVFEKDDNSPRYADNEVNSNSRKQHKH

Target ORF information:

RefSeq Version	NM_001043250.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster myosin heavy chain-like, transcript variant G (Mhcl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043250.2

ORF Insert Sequence:

ATGTTCAACT TTATGAAGAA GAGCGCTGGG GATGAGGACA AGGAGCGTCG CAAGCGCGAA 
AAAAAACTGC GCAAAGAGGC TAAGGGCACC TCTGGTGGCC CGGGAATGGG AGGCGTGACT 
GGCGTCACCG GCAGCATGAG CACCGACGAG CTGCTCCGCC TGGACGAGGT GCGCCGATCG 
CTTAAAATCC GAGGGCGACG CAAGGAGAAG GAAAAGTTGC CGAGCGGAAT TACGGCGGAC 
TATAGCGCAG ACTTCTTTGC CGCCCTCAAT GCAGATGCAA ACGGCGGAAG CAACGGAGGA 
GCAGTGGGAG CTGCAGATCC CGAGCAGGAT CGTGGCAATG AGGAGATTGT AGCATCTGTG 
ATGACGCACA TCGAGAGCCG AGCGTCCAAT GGCGCCGGCG TGAGTGTCAA CTACAGCGAG 
GTCACACATT CTCTCCTCTC TGGAGGGCTC AAGAATCGCT TTCTTCCACC TATACCCCCA 
AAACCACCCA AGCGTGGCAT TCTGAAAGGC TCCCGCAGTA ATATGACCAA TGTCCACGAG 
GAGATTAGCT TTGCGGGTAG TGCCGGAGGA GCAGGTGGCA CGCCAGAGAT GCTATTGCGC 
AACACCTTTC AGAATGAATC CCTTTACGAG GGAGCTGCCA GGGGAGCTGG TTCGATCGGC 
TCGAACTCCA GTCAGCATGG AACCTCGTTC ACTTCCGTGG AGAGCCTGCG CAGCGATGGA 
GAGCAGACAG GAGGTCAACA ACGGGCCATG ACCCTGCCGG CCAACGCCCG CTATGGAGCT 
TTACCCCAGG GCAACAGTCA AGGATCGCAC CTCCATGTGC TCACCTCGCC GTCGCCCAGC 
GCCGACAGTC TCACAGATAC CACAAACTCC TCTTTCGCCA CACCCCCATT TTCGCTGAGT 
CCCGTCGGCG AGTCCCAAGG CATCGACCGT TGGGCCCGCG TCCATGCCTT CGAGGATGTG 
GAGCTGCCGC TGCCGCCAGT GCAGCTAGTA AAACTTCCCC CACCAAGGCA GCTAGTGATC 
AGGCGACAGA AGTCTCCGCG CCAGGACTTC GGCTTTAGCC TGCGCAAGGC CATCTGTTTG 
GACAGAACAG AGTCCCTGAC TTCGCCCATT TTCAGGCCGG TTATATTCGC CGAGCCAGGT 
GCTGGTGGCG GAGCCACAGG TCTTTTGCCT GGCGATCGCC TAATCAAGGT CAACGGCACG 
CCGGTTGGGG AACTGCCGCG GGAGATCATA ATTGAGATGA TACGGAACAG CGGAGAGGCT 
GTCACCGTGG AGGTCCAACC AGTCGCTGAG TTGGTGGAGC TGAGCAAGCG ATGCATGGCC 
CCCAGCACAG CCACCGTGGA GGAGATCGAT CATTCCATTA CCAACGGCAA CTGCAATACC 
CTGCGACGCA GTGCCAGCAA GCGCTTCAAG CGTCAGAGCC GCCATGAGAA TGGCAATGGC 
GGAGGAGAAG AGGGAGGAGC CAAAGGAGTG GGCCACGATC TGGAGGCGGA TGTTGCGGAT 
GCCAGTCAGC CGGAGCGCGT ATGGCTGGTG CACCGCGGTG GTTTCACTGC CGCCATCCGT 
CTGCCGACTG CGTCTTCTGG CCGGGATGAG GAGAACAAGC TAAGCCTGCG CCTCCTGCAT 
AATGGGGAGC AGCTGACCGT CGACGAGGAC GATGTCGAGA AGCAGAACAG CCCCGCCCTG 
GACCTTGTCG AGGACATCTG CGAGCTGAAG TATCTGAACG AGGCGTCCGT GCTCCATTGC 
CTGCGTCAGC GATACGCCAG CAATCTCATC CACACCAAGG CGGGGCCCAC GCTGCTCGTG 
GTCAATCCCA TGGCACCGCT GTCCCTGTAC TCCGAGAAGG TTGTCTCCAT GTTCCGCGGC 
TGCAAGACGG AGGATATGCC GCCCCACGTC TATTCACTGG CCCAGACCGC CTACAGGAGC 
CTGGTCGAGA CGCGGCGCGA CCAGAGCTTG ATATTCATGG GCCGATCTGG TTCTGGCAAA 
TCCACTAGCT TCAAGCATGC ACTCAACTAC CTGGCGTTGG CAGCTGGTGC CTATAATAAT 
TTTATAAACG CGGAGAAGGT CAATGCCCTG TGCACCATTC TGGAGGCTTT TGGAAATACG 
AAAACTTGCC TAAACTCGAA TGCCACGCGG ATGACGCAGC TGTTAAGCCT GGATTTCGAT 
CAAACCGGCC AGATTGCATC TGCCTCATTG CAAGTTCTCC TCCCAGAAAG ACAACGAGCT 
GGCCGACGAC TTGGTCATGA ACATAGCTTC CACATCATGA CGAGACTTTT GGCTGGCGCT 
GCTGGCTTGC TACAGAAGGA GCTGCACCTG GAGAACATAA CCTCTGAGGA CAGCCATCCT 
TTTATATCGT TATCCCAAAA GCTGGAAGAT CGGCATCGAG CTGCCAATGA CTTTATGCGC 
ACTGTTCAGG CCTTTGAGAC GCTGAACATT GATGCGAAGG CGGTACGTGG GATCTGGAGC 
ATTTTGGCGG CCATTTACCA TCTGGGCATT GCTGGCGTCA CAAAACTGGG CACTGGCTCC 
ACAGCCCGAA CTCAGTTTGC CAATCCCACG GCCGCCCGAA AGGCCTCGGG CTTGTTGGGC 
GTGAATCTGG AGGATCTGTC ATCAGCTGCC TTTGGTCTTA CCCAACCAAA CGCCCCCAAT 
GGCGGTCTGA GTCCATCGAA ATCGCCCACC TCGGACACTG GACACGAATG GGCCTGGGAA 
TGTCTCGAGG CGTTGGTCAT TGGTCTATAT TCGGAGGCCT TGGCTGCAGT GGTGGCATTG 
ATCAATCGTC AGATCTGCAC ATCGTCCCAC ACCATAGCCT CAATTATGCT AATTGACACG 
CCCGGCTTCC AAAATCCAGC CAGCTGTGGC CAGCAAGTGG GTGCTACTCT AGCGGATCTG 
CGTCACAACT ATCTACAGGA GCGTCTACAG ATGCTCTTCC ATCACACCAC ACTGGTGGCT 
CCCCGCGATC GCTACGCCCA GGAGCTGGTA GAAATCGAGA TGGACCTGGC ATCGGAGTGT 
CATCCGGGAC CGTTGATCTC GCTTATCGAC AAGGCACCTC AGAATCATGT AGTGCGCTCG 
TCCCAGCGAG ATTTGCGAGA GCATGATCGT CGAGGAATGT TGTGGCTACT GGATGAGGAA 
GCTATCTACC CCAACTCCAA CGATGACACA TTCCTTGAAC GTTTGTTCTC CCACTACGGT 
GACCGGGAAC ATCATAGCCT GCTGCGCAAA TGTGCAGGTC CGCGACAATT TGTACTCCAC 
CATCTGCAAG GCACCAATCC CGTTCTCTAC GCTGTCGATG GATGGGTGCG TCATAGCCGG 
GAGCACCCAG GTATTCGGAA TGCTGTATCC CTACTACAGG ACAGCAGTCG GGAGGAGATC 
AATCGCCTAT ATATCGGCTC ACTGACTCGA GGATCGGGAG CAATGGTGTT TTGTGGCTCT 
TTTGCTGGAC TGGAAGGCAC TCAGTCACTT CGTCGCGTGT CCAGTATTCG CCGCTCCTTC 
ACAACGGCCG GAGTAAAGCG AAACTCCATA ATGCTGCAGG TGAAGTTCAC AGTGGATGGA 
ATTATCGACA CTTTGCGCCG CACTGGAACT CACTTTGTGC ACTGCTATCT GCTGCAACAT 
AACGCAGGCA AACATACCAA GTACACCGCC AATGGATCGC CCAGCTCAGC TGCCGGTCAG 
GTGTCCAGCG AGGAGGAAAT GGTCAACGTG CCACTGTTAA GGAGTCAGCT ACGTGGTTCA 
CAAGTCCTAG AAGCGGCTCG CTTGCATCGC CTTGGTTTTC CCGAATCAGT GCCATTGTTG 
GAATTTGTCA GGCGTTTCGG GCTTTTGGCC GGGGATTTGG CTAGCAATAA GGATGTGAGC 
GTGGAGCAGA TATTAGCTGT CAATGAACTG GATGTGGCCA GCTATCGCAT AGGACCCAGC 
CAGATCCTCT TCCGCTCAGG AGTTCTTAGT GAACTGGAAG CCAAGCGCGA TGTCCTGCTC 
TCAGATCGCA TTATACAGCT GCAAGCATTT TGCCGCGGCT ACTTGGCACG CAAGAAGATG 
TCCCAACGAA GGGTCCAGGA ACTGGCAGTG CGTTGCATTC AACGCAATGT GAAGGCATTC 
CTTGCCGTTC GCGACTGGCC GTGGTGGCGT CTCCTGGTGC GGGTCACTCC CCTGCTCAAC 
GTTCATCGCA CCGAGGAGCA GCTGAAGACG GCTAACGAGG AGCTCCTAAT GCTGCGAGCC 
AAGCTGGAGA AGATCGAGTG TGATCGCAGT GAGGTCAAGG CAGAAAACCA AAAGCTGGAA 
GCCAAGCTAT CCGAGCTGAC GGTGGACCTG GCCGAGGAGC GATCCACGGC TCATATAGCT 
ACCGAGCGGC TGGAGGCGGA AACCGCCGAA CGCTTGAAGC TGGAGAAGGA GCTGGGCGAT 
CAAACCAACA AGGTGAAGAA CCTTCAGGAG ACGACGGAGA AGCTGGAAAT GGAGCTGATA 
TGCGCCAAGT CCGATCTGAA TGGCATCTCT GAGGACGAGG ATGCAGAGAA CGAGGATGGG 
GTCGGCGGCG GCGTCTACAA GCTTAAGTAC GAGCGGGTGG CCAGGGAGCT GGAGTTCACC 
AAAAGGCGGT TACACACGCA GCATGAGCAC GATCTGGAAC AGCTGGTCGC GCTCAAGAAG 
CATTTGGAGA TGAAGCTTTC CGATGCCTAC GAAGAAGTTG TGGAACAACG TCAGGTTGTG 
GGCCAATGGA AGCGCAAGGC ACAGAAGATG ACCAACGAGA TGAACGATCT GCGCATGCTG 
CTCGAGGAGC AAAATGCGCG CAACAATTTG CTCGAGAAGA AGCAGCGCAA GTTCGACGCC 
GAGTGCCAGT CCCTGCAGGA TGCAGTGCGT CAGGAGCGAC AGGCCAAAGA GCGCTACGGT 
CGCGAAAAGG ACGTCCTGCA GGCCGAGAAG TTCACACTGG AGCAAACGCT GGCGGACACC 
CGTCTGGATC TTGAATTCAA GGAGGAGAAA CTTGCATCGC TACAGCGCGA GCTGGAGGAG 
ATGACCTTTG GTGGCGGCAC CGAGGAGGAG TTCGCCCAAC TGCGGCGCTC CAAAAATGAA 
ACAGAGCGTC GGGCCAAGGA ACAGGAGGAA GAACTGGACG AGATGGCCGG TCAGATACAG 
CTGCTCGAAC AAGCCAAGCT TCGCCTGGAG ATGACCCTGG AAACAATGCG CAAAGAAGCG 
CGCCGTGAGT CCCAGCAGCG CGACGAAGAG CTGGAAGAAG TACGCGGTAA CGGCTACAAG 
AAGATTAAAG CCCTTGAATG CCAGCTGGAA ACTGAGCACG AGGAGCGGAC TCTGCTGCTG 
CGCGAGAAGC ACGAGCTAGA GCGACGTCTC TCCTCCATGG AGGATCGCGA TCGTGTTGAT 
CGCGATGCCG AGGAAGCGCT CAACCAAAAA CTGCGACGCG ATCTTCGCAA ATACAAGGCC 
CTGCTCAAGG ACGCCCAGAC ACAGTTGGAG CGGCTTAAGG CTGACACGCC TGGCAAGACG 
CTTATAAGAC AACTGCGTAA TCAACTGGAG GATGCCGAAT CTGCTCGTTC GCTGGCTATG 
AAAGCGCGAC AAACTGCGGA AGCCGAACTT ACTGAAGTGC AGGCCATGTT CGATGAGTCG 
CATCGAGCTA GGAATGACGC GGAGGAGCGA GCCAATGCCG CACACAGAGA TCGGGCTGAG 
CTGCAGGCCC AGATCGAGGA GAACGAGGAG GAGCTGGGCG AGCTGATGAA GAAGTACAGC 
GCCACGGTAA AGCAACTAAA TACAGAGCAA ATCAACGTAT CCGAGGCTGA GTTTAAACTC 
AATGAAATGG AAGCAGAGCG CAATAACCTC AAGGAGCAGG TGGCCGAGCT GCAGCACCGA 
CTGGACAACG TTGAGAACTT GGGGGATCCA TCTATGGCCA TGATGTCCAA GCGACTGGAG 
CTGCGCACCA AGGAATTAGA GTCTCGACTG GAACTAGAGC AGGCCACTCG GGCTCGCCTT 
GAAGTGCAGG TGAACCGTCA CAAAGAGGCT CTGGAAAAAC TGCAGAACGA GGTGACGCAG 
TCAAAGATGC GTGAGATGCA GGCCCAGGAT GTAATCAAAA AATCGCAAAA GAGTCTGCGC 
GACATGCGCG AAGAGTTCCA CGCTGTCTCC AGCCGCGAGC AGGAGTCGCT CACAAGGCGC 
AAGGACCTGG AGAAGAAGGT GGAGCAAATG GAGTCGGAGG GAGCGGCGCT TAAAAACGAT 
CTACGACTGG CTTTACAGCG AATAGCTGAT CTACAGCAGG CAATGGAGGA AGAGGGCGAG 
GAGGAGCTGA GCGAAAGTGA TGAAAGCCTC AGTTCGGTGG GCTCTATCAG TGATCTGGAA 
GATCGACTTC GGCCAGTTCA TGTAAAACGC AGCTCACAGC AGTCCTTGAA CGGCAGCATC 
GGCGGCGGAG GCGGAAGCGT TGTCAGCTCC ACCCGCACCG TGGTGTTCGA AAAGGACGAC 
AACAGCCCGA GGTACGCTGA TAACGAAGTC AACTCGAACA GCAGAAAGCA GCACAAGCAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mhcl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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