msps cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	msps
Entrez Gene ID	41952
Full Name	mini spindles
Synonyms	CG5000,Dis1,Dmel\CG5000,MSPS,Msps,TOG,XMAP215,anon-WO0140519.136
General protein information	Preferred Names mini spindles Names CG5000 gene product from transcript CG5000-RD CG5000-PB CG5000-PC CG5000-PD CG5000-PE CG5000-PF mini-spindles minispindles msps-PB msps-PC msps-PD msps-PE msps-PF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 89B1-89B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001260201.2	NP_001247130.1	mini spindles, isoform E
NM_001260202.1	NP_001247131.1	mini spindles, isoform F
NM_001260200.2	NP_001247129.1	mini spindles, isoform D
NM_169698.3	NP_732105.2	mini spindles, isoform C
NM_001170156.2	NP_001163627.1	mini spindles, isoform B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CKAP5	XP_005631330.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011162	XP_309487.4
Rattus norvegicus (Norway rat)	Ckap5	XP_006234666.1
Xenopus tropicalis (tropical clawed frog)	ckap5	NP_001120559.1
Homo sapiens (human)	CKAP5	NP_001008938.1
Pan troglodytes (chimpanzee)	CKAP5	XP_001165890.1
Oryza sativa (rice)	Os01g0816400	NP_001044618.1
Arabidopsis thaliana (thale cress)	MOR1	NP_565811.2
	CKAP5	XP_002808118.1
Bos taurus (cattle)	CKAP5	NP_001178292.1
Mus musculus (house mouse)	Ckap5	NP_001159461.1
Gallus gallus (chicken)	CKAP5	NP_001186575.1
Danio rerio (zebrafish)	ckap5	NP_001032756.2
Drosophila melanogaster (fruit fly)	msps	NP_001163627.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Msps tubulin overexpressed gene domains have distinct architectures that bind either free or polymerized tubulin, and that a polarized array drives microtubule polymerization.
Title: TOG-tubulin binding specificity promotes microtubule dynamics and mitotic spindle formation.

Drosophila melanogaster mini spindles TOG3 utilizes unique structural elements to promote domain stability and maintain a TOG1- and TOG2-like tubulin-binding surface.
Title: Drosophila melanogaster mini spindles TOG3 utilizes unique structural elements to promote domain stability and maintain a TOG1- and TOG2-like tubulin-binding surface.

Findings suggest that Msps is a novel Rac effector that acts, in partnership with Orbit, to regionally regulate microtubule dynamics.
Title: TOG Proteins Are Spatially Regulated by Rac-GSK3? to Control Interphase Microtubule Dynamics.

MT dynamics are promoted by the independent as well as the cooperative action of XMAP215(msps) polymerase and the EB1-Sentin duo.
Title: Reconstitution of dynamic microtubules with Drosophila XMAP215, EB1, and Sentin.

Msps exhibits EB1-dependent and spatially regulated microtubule localization, targeting to microtubule plus ends in the cell interior and decorating the lattice of growing and shrinking microtubules in the cell periphery
Title: The microtubule lattice and plus-end association of Drosophila Mini spindles is spatially regulated to fine-tune microtubule dynamics.

The following msps gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the msps cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa05078	NM_169698.3 Latest version!	Drosophila melanogaster mini spindles (msps), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa08419	NM_001260200.2 Latest version!	Drosophila melanogaster mini spindles (msps), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa08420	NM_001260201.2 Latest version!	Drosophila melanogaster mini spindles (msps), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa08421	NM_001260202.1 Latest version!	Drosophila melanogaster mini spindles (msps), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OFa08421	NM_001170156.2 Latest version!	Drosophila melanogaster mini spindles (msps), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

msps ( NM_169698.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732105.2 Drosophila melanogaster mini spindles (msps), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa05078
Clone ID Related Accession (Same CDS sequence)	NM_169698.3
Accession Version	NM_169698.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mini spindles, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169698.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGAGG ACACAGAGTA CAAGAAGTTG CCGGTGGAGG AGCGGTGTGT CCATAAACTA 
TGGAAAGCCC GCGTCGATGG TTACGAGGAG GCCGCAAAGA TCTTTCGCGA GCTGGATGAC 
GAGAAGTCGC CGGAATGGTC TAAATTTGCA GGGCTCATAA AGAAGATGGT AGTGGACTCT 
AATGCTCTGG CCCAGGAAAA GGGTCTAGAA GCGGCCCTCA TTTTTGTGGA GAACAGCGGG 
CTGGCGGGAC GCACAGTAGG CGACGTGATG ACAGGCATCG TTCAGAAGTG CATTGCGGCG 
CCAAAGACCA AAACCAAGGA GCTCTCCGTC CAAGTGGCTC TAATGTATGT GGAGATAGAG 
AAGCAGGAGG CCGTCGTCGA AGAGCTGGTT AAAGGTATGG AGGCCAAGAA CCCTAAGATT 
GTGTCTGCCT GCGTGGCAGC CACCACACTG GCCCTCCGCG AGTTCGGACA CAAGGTGATC 
GGCGTCAAGC CGTTAATTAA AAAGCTTGCT CCGCTAATGT CTGACCGAGA CAAGACGGTC 
CGAGATGAGG GCAAGCAGCT GGCTGTTGAG ATTTACCGTT GGATCGGAGC TGCAATGAAG 
GCACAGATCT CAACGCTACC ACAGGTAACG CTAAAGGAGC TGGAGGATGA ATTCGACAAA 
CTCAAAGGTG AACGCGTTGA ACCCTCCAGA TATCTAAAGT CCCAGCAGGA AAAGCAAGCG 
AAGATCGCCG ATGCTGCAGC GACCGAGGAT GCATATAATG AGGATGATGG CGAGGCTGGC 
GTTGAAGAAA TCGATCCTAT GGATCTTCTA GACCCCGTTG ACATTCTCTC CAAAATGCCC 
AAGGACTTCT ACGATAAGCT GGAGGAAAAG AAATGGACTC TGCGCAAAGA ATCGTTGGAA 
GTCCTGGAGA AACTGCTCAC TGACCACCCC AAGCTAGAGA ATGGCGAGTA TGGAGCGCTG 
GTAAGCGCTC TTAAAAAGGT TATAACCAAG GACTCCAACG TAGTCCTCGT TGCAATGGCC 
GGAAAATGTC TAGCCTTGCT AGCCAAAGGA TTGGCAAAGC GATTCTCAAA TTACGCATCC 
GCTTGTGTCC CATCCCTGTT GGAAAAATTT AAAGAGAAGA AACCTAATGT TGTGACAGCG 
CTGCGTGAAG CTATTGATGC CATATACGCC TCCACTTCTC TAGAGGCCCA GCAAGAGTCC 
ATTGTAGAGT CCCTGTCCAA CAAAAATCCA AGTGTTAAGT CCGAAACGGC CTTGTTTATT 
GCCCGTGCCC TAACCCGCAC CCAACCAACC GCGCTAAACA AAAAGCTGCT TAAACTTCTG 
ACCACCAGTC TAGTGAAAAC GCTTAACGAA CCAGATCCAA CCGTACGCGA CAGCAGCGCC 
GAAGCTCTTG GTACTTTAAT AAAGCTAATG GGAGACAAAG CGGTGACTCC TTTGCTCGCC 
GACGTCGATC CACTCAAAAT GGCCAAGATA AAGGAGTGCC AAGAAAAGGC GGAGATTAAA 
ATAAAGGTTG CAGGTCCAAA GAAGGAGACA CGACCTGCAT CTGCACCGAC TGCGAAAGCT 
GCTGCTCCGG CTAAGACAGT GGCTGGTAGT GTGGACCCTA AGCCAGTAAC CCGACCGGCC 
ACTACAGGGG CACGTAAGGT CTTGAAAAAA CCGGCGACGG TTAGTGGAGG AGGAGCTACA 
TCTGCCCCAA CTGCAGCTCT TAAAGCGGGT GGAAAACCCC TAGCCACGGA ACGCGAAATA 
ACCCCCGAGG AATTGCAAGA AAAATCCGAA GAAATTCTGC CAGCCGAAAT TCTTAATGGG 
CTGGTGGATT CCAACTGGAA GAACCGCCTT GCTGCTGTGG AACAGCTTCT GGGCGAAATA 
AGCGGTTTTG ATGCAAAACA GGCTGGCATA TCACAGATCC TGATCCGAAC AATCAGTGGA 
CGAAAGCCAG GCCTCAAAGA AATGAATTTC CAGGTACTCA AGTTTAAGCT AGACATAATC 
CGCAGTGTGG CTGAAAACTA TCCACTGACC ACGACAACCG TTGATCTGGT GATTAACGAA 
ATAATCGAGA AGCTAGCAGA CGCCAAGAAT GGTGCAGCAG CTGCAGATGT TTTGTCTGCC 
TTTGCCGAAG CCACTAAACT GGAGTATGTT GTGGGCAAAG TATTGAGCTT TGCTTTTGAA 
CAAAAATCGC CTAAAGTGCA ATCAGAGGCC TTTAACTGGG TTAACAGGTC AATCATAGAA 
TTCGGCTTCC AATTGCAGCC AAAGACACTT ATTGAAGATG TACGAAAAGG CGTGCAGAGC 
ACGAATCCAA CGGTTCGTGC TTCCGCCATT CAAATGGTAG GCACCATGTC TATGTATATG 
GGCAAAGCTC TAATGATGTT CTTCGACAGC GAGAAGCCTG CACTAAAGTC CCAGATCCAG 
GTAGAGTTTG ACAAGAATGT GGGCGAAAAA CCACCCAAGC CTGTTAGAGG AGTTCAACGT 
AGCAGCGGTG GAACCGCTGG TAATTCCCCA GACAATGAGG ACGATGATGG TGGGGCAGCC 
GGAGAAGAGG AGCCCATTAA TATGGCTGAT CTTCTACCAC GCGTTGATAT TGCTCCACAG 
ATTACGGAGG CATTGCTGAA GGAGATGTCC GACAAGGACT GGAAAACTCG CAACGAGGGT 
CTGACTAAGC TGCAGGCTAT TATTTCAGAG GCGCGGCTAA TCAAACCCAG CATTGGCGAC 
TTGGCTCCTG CTCTGGCCCA CCGTCTGGTA GATTCCAATG CTAAGATTGC CCAAACGACA 
CTTGCCATTT GCGAGCAGCT TGCCACAGCC ATGGGGGCTG GTTGTCGAAA TCACGTGCGG 
AACTTGTTCC CTGGCTTTTT ACACGCTCTG GGTGATAACA AGAGTTTTGT AAGAGCCGCT 
GCCCTCAATT GCATCAATAG TTTTGGCGAA AAGGGCGGCT ATAAGGAATT TTTTGAGAGC 
GAAATGATAG CCGATGCCCT GAAAGGAGGA TCCCCAGCTC TAAAGACAGA ATTGTGGGCT 
TGGCTGGCAG ATAAGCTGCC TGGTCTTCCC CCCAAGTCAG TATCGAAGGA GGATATCCAT 
TCAATGGTGC CGCACTTATA TGCTCACATC TGCGATCGAA ACGCCGATGT CCGGAAAAAT 
GCCAACGAGG CGGTGTTGGG CATTATGATT CACCTTGGAT TTGATGCAAT GAACCGCGCT 
CTGGACAAAC AGAAGCCCGC GTCCAAAAAG GATATACTTG CAGCACTCGA AAAAGCGCGT 
CCTAATCTTC CGGTAAAACC GCTGCCCAAA GGCAAGCATC AGGCTCCAAT ACCTGAGGAG 
CCGAAGCTAA AAACAGTGCG CGGCGGTGGA GCAGGTGGTG CGCCTGGAAT CCAGAAGAGT 
GCTACAGCCC GGGTTGCCGG AGGACAAGAC AAGCAGGTAC CAGCGCGCAA AAAGGACGAA 
GATATCGACA CATCGCCGCT ACTGTGCGCC AACAGTGCTA AAAACCAGCG GCTGCTAGAC 
GAGCAAAAAA TGAAGGTACT AAAGTGGACT TTTGTAACCC CAAGAGAGGA ATTCACCGAA 
CTGTTACGCG ACCAAATGAT GACCGCAAAC GTAAATAAAG CACTGATAGC CAACATGTTC 
CACGACGATT TTCGCTACCA CTTGAAAGTC ATCGAGCAGT TGAGCGAAGA TCTGGCTGGT 
AACAGTAAGG CGCTGGTGTG TAATCTGGAC CTAATACTTA AATGGCTGAC GCTGCGATTT 
TACGATACGA ACCCTTCTGT GTTGATTAAG GGTCTCGAAT ATCTAGTACA GGTTTTCCAG 
GTACTCATTG ACGAGGAATA CATTCTGGCC GAGAACGAGG GAAGCAGTTT TGTCCCACAT 
CTGCTTTTAA AGATCGGAGA TCCAAAGGAT GCTGTTCGAA ATGGCGTACG CCGCGTTCTG 
CGGCAAGTAA TATTGGTCTT CCCTTTCGTC AAGGTCTTTG GCTATGTGAT GGAGGGTCTA 
AAATCGAAGA ATGCCCGTCA GCGTACTGAG TGCCTTGACG AGTTAACCTT CCTAATTGAA 
AGCTATGGCA TGAACATCTG TCCCCAATCG GCGGTGCGCG AGATTGCTCG CCAGATTTCC 
GACCGGGATA ATTCCGTACG CAACGCAGCA CTCAACTGCA TCGTCCAGGT CTTCTTTTTG 
TCCGGGGAAA AGACTTACAA GATGATTGGC CATCTTAACG AAAAGGACCT CTCAATGCTA 
GATGAGCGCA TCAAGCGAGC CAAAAAGACT AAGAAGCCCA CGCCACCGCC ATCTGTTGAT 
GTGCCGGCAC CTCAACGACA TGACAGCATC GAAATCGAAG ATGCTGAAGT GGGCAACGGT 
TGCGACGAGC TTCCACCACC AGATGAGGAC GGAACTTTCG ACCAGGCACC TTCGTCACAG 
CTGCTTCTAC TCCAGCAACA GCTTCAACAG TTACAACAGC AGGCCCAGCA ACAGAAGCCC 
AGCGGACCAT TCGGCTTGGA CTCGCAGGTA ATCAGCGAGA TCGAAAAAGA TTGGGTCCGA 
GTGGACCAAA TGGAGCAGAA GCCGCTACTG AACGTAGATA TCTCATCGCT GGACGAGCCT 
ATTAAGGTGA GGCCCACGCG GGCCGGCATT CACTACCCGC AGGAGAAGTT CGATCGGCTG 
ATTTCTCGAC AACACTATAT GCAGCAGACT TTAACGACGT CCCCGTCATC CACGGCTGGA 
ATGACCAGTG GAGTCTCCCC GTATCGCAGT CCTATGCGCT TGCAGCACCA GCAGCCTCAA 
CAGCAGCTGG AGAACAATAT ACCAAATTTG GCGGATGTTT TGCCAAAGCA CGATCCGCAG 
CTGGTAAAAG TTATAAAAGG TGTAAGCAGC ACGGACACTC TAAAGGCGCG CGCGGCTATT 
AACGAGCTTG CCGCCATCAT CGAGGCTCCA GAGAAGCAGG CTGTGCTGCG CGACTATGAA 
GAAATATTTA TTCAGAATGT GCTTGCACAG TTTAAGAATC TCTCACAAAT ACCATCGGCT 
CAATCCGTAG TTGTTTACCA GCCGCTATTA TCCATTCTGT ATACATTTTT CCATGCCAAC 
ATTCTGGGCA AAACGCTGAG CGTGGCCTGC ATCAAAAATC TAATGTCGGC GCTGTTGAAC 
CTTATGGCTG ATCCGAAGTT GGCCGTGGGT GATGATAGTC AGTATAACAA GGTTATTAAT 
GGCATATGTC TAAAAGTGTT GGACAAGGTA GATTTTACGA ATTTAAACTG TGCTTTGATA 
CGCCTATTAC GGGAGACTTG TCCGGAGGCA AAGCTGCCGA AGTTTACAGA CCTGCTGATG 
AAGTGCATAT GGCGCAACGT TAAGATGCTA CCCGAACGAA GCAACGAGCT GAACTATGAC 
GCCGTAATTC TCGAGGTGCA CGAGTTCATG CTCGCGCTGC CTAGCACTTG GTGGCAAAAC 
CGACCGTCGG ACACTCCAAT GCGCACGATT AAGACCATTC TGCATAACAT GGCAAAGGTG 
AAGGGGAATG CTATTCTGCA GCATCTCAAC CAGATTCCAA CACACTCGGA GCTACATACT 
TATTTGATAC GCATTCTCAA GAATTTCCAA AAGGATGGCT CTGCTTCAGG AATTGGTGCC 
TCCCCCCAGA GGGCCAAGGA GATTGCCTCC AAGCGAATTT CGCACCAAAC ACATGATACA 
GTATCACAGA TCTTTAAGCT GATCTCAGAT AGAGATACCA AACAGCAAGG TCTGCAGAAA 
CTTTACGACT TTAAGCAACA AAATCCTGAT ATCGATCTAA GTACCTTTCT GCAAGGCTCC 
AGTGCCCCAT TTCACAAGTA CATCGAGGAG GGTTTAGCCG AGATTGAGAG GAATCAGAAC 
GCTGGATCCA CACAGGATAA TCGGACGGAT GTCAACTACC AGAACAACGG TCCCGATCCC 
GATTTCTGGA TGGACCGCCT GCAGTATCAT ATGACCGGAG GGGCCGCCAA GCTAGCATCT 
GCACGTTCAG CAGACGATGG ATCCCATATG CTGGACAATA AGGTGGTTGA CGAGAACCTC 
TGCCTGAACG GAATGAACGC GCAAAAGGCG TCGCTCATTA AGCGAGAAAA GCGGGACATG 
TCACCAAATC GCTTGCAGCA TCTCCAGGCA AAGCTGGCTC AAATCAAGAA AGAGAATCAT 
GCCCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732105.2
CDS	447..6575
Translation	MAEDTEYKKLPVEERCVHKLWKARVDGYEEAAKIFRELDDEKSPEWSKFAGLIKKMVVDSNALAQEKGLEAALIFVENSGLAGRTVGDVMTGIVQKCIAAPKTKTKELSVQVALMYVEIEKQEAVVEELVKGMEAKNPKIVSACVAATTLALREFGHKVIGVKPLIKKLAPLMSDRDKTVRDEGKQLAVEIYRWIGAAMKAQISTLPQVTLKELEDEFDKLKGERVEPSRYLKSQQEKQAKIADAAATEDAYNEDDGEAGVEEIDPMDLLDPVDILSKMPKDFYDKLEEKKWTLRKESLEVLEKLLTDHPKLENGEYGALVSALKKVITKDSNVVLVAMAGKCLALLAKGLAKRFSNYASACVPSLLEKFKEKKPNVVTALREAIDAIYASTSLEAQQESIVESLSNKNPSVKSETALFIARALTRTQPTALNKKLLKLLTTSLVKTLNEPDPTVRDSSAEALGTLIKLMGDKAVTPLLADVDPLKMAKIKECQEKAEIKIKVAGPKKETRPASAPTAKAAAPAKTVAGSVDPKPVTRPATTGARKVLKKPATVSGGGATSAPTAALKAGGKPLATEREITPEELQEKSEEILPAEILNGLVDSNWKNRLAAVEQLLGEISGFDAKQAGISQILIRTISGRKPGLKEMNFQVLKFKLDIIRSVAENYPLTTTTVDLVINEIIEKLADAKNGAAAADVLSAFAEATKLEYVVGKVLSFAFEQKSPKVQSEAFNWVNRSIIEFGFQLQPKTLIEDVRKGVQSTNPTVRASAIQMVGTMSMYMGKALMMFFDSEKPALKSQIQVEFDKNVGEKPPKPVRGVQRSSGGTAGNSPDNEDDDGGAAGEEEPINMADLLPRVDIAPQITEALLKEMSDKDWKTRNEGLTKLQAIISEARLIKPSIGDLAPALAHRLVDSNAKIAQTTLAICEQLATAMGAGCRNHVRNLFPGFLHALGDNKSFVRAAALNCINSFGEKGGYKEFFESEMIADALKGGSPALKTELWAWLADKLPGLPPKSVSKEDIHSMVPHLYAHICDRNADVRKNANEAVLGIMIHLGFDAMNRALDKQKPASKKDILAALEKARPNLPVKPLPKGKHQAPIPEEPKLKTVRGGGAGGAPGIQKSATARVAGGQDKQVPARKKDEDIDTSPLLCANSAKNQRLLDEQKMKVLKWTFVTPREEFTELLRDQMMTANVNKALIANMFHDDFRYHLKVIEQLSEDLAGNSKALVCNLDLILKWLTLRFYDTNPSVLIKGLEYLVQVFQVLIDEEYILAENEGSSFVPHLLLKIGDPKDAVRNGVRRVLRQVILVFPFVKVFGYVMEGLKSKNARQRTECLDELTFLIESYGMNICPQSAVREIARQISDRDNSVRNAALNCIVQVFFLSGEKTYKMIGHLNEKDLSMLDERIKRAKKTKKPTPPPSVDVPAPQRHDSIEIEDAEVGNGCDELPPPDEDGTFDQAPSSQLLLLQQQLQQLQQQAQQQKPSGPFGLDSQVISEIEKDWVRVDQMEQKPLLNVDISSLDEPIKVRPTRAGIHYPQEKFDRLISRQHYMQQTLTTSPSSTAGMTSGVSPYRSPMRLQHQQPQQQLENNIPNLADVLPKHDPQLVKVIKGVSSTDTLKARAAINELAAIIEAPEKQAVLRDYEEIFIQNVLAQFKNLSQIPSAQSVVVYQPLLSILYTFFHANILGKTLSVACIKNLMSALLNLMADPKLAVGDDSQYNKVINGICLKVLDKVDFTNLNCALIRLLRETCPEAKLPKFTDLLMKCIWRNVKMLPERSNELNYDAVILEVHEFMLALPSTWWQNRPSDTPMRTIKTILHNMAKVKGNAILQHLNQIPTHSELHTYLIRILKNFQKDGSASGIGASPQRAKEIASKRISHQTHDTVSQIFKLISDRDTKQQGLQKLYDFKQQNPDIDLSTFLQGSSAPFHKYIEEGLAEIERNQNAGSTQDNRTDVNYQNNGPDPDFWMDRLQYHMTGGAAKLASARSADDGSHMLDNKVVDENLCLNGMNAQKASLIKREKRDMSPNRLQHLQAKLAQIKKENHAQ

Target ORF information:

RefSeq Version	NM_169698.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mini spindles (msps), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169698.3

ORF Insert Sequence:

ATGGCCGAGG ACACAGAGTA CAAGAAGTTG CCGGTGGAGG AGCGGTGTGT CCATAAACTA 
TGGAAAGCCC GCGTCGATGG TTACGAGGAG GCCGCAAAGA TCTTTCGCGA GCTGGATGAC 
GAGAAGTCGC CGGAATGGTC TAAATTTGCA GGGCTCATAA AGAAGATGGT AGTGGACTCT 
AATGCTCTGG CCCAGGAAAA GGGTCTAGAA GCGGCCCTCA TTTTTGTGGA GAACAGCGGG 
CTGGCGGGAC GCACAGTAGG CGACGTGATG ACAGGCATCG TTCAGAAGTG CATTGCGGCG 
CCAAAGACCA AAACCAAGGA GCTCTCCGTC CAAGTGGCTC TAATGTATGT GGAGATAGAG 
AAGCAGGAGG CCGTCGTCGA AGAGCTGGTT AAAGGTATGG AGGCCAAGAA CCCTAAGATT 
GTGTCTGCCT GCGTGGCAGC CACCACACTG GCCCTCCGCG AGTTCGGACA CAAGGTGATC 
GGCGTCAAGC CGTTAATTAA AAAGCTTGCT CCGCTAATGT CTGACCGAGA CAAGACGGTC 
CGAGATGAGG GCAAGCAGCT GGCTGTTGAG ATTTACCGTT GGATCGGAGC TGCAATGAAG 
GCACAGATCT CAACGCTACC ACAGGTAACG CTAAAGGAGC TGGAGGATGA ATTCGACAAA 
CTCAAAGGTG AACGCGTTGA ACCCTCCAGA TATCTAAAGT CCCAGCAGGA AAAGCAAGCG 
AAGATCGCCG ATGCTGCAGC GACCGAGGAT GCATATAATG AGGATGATGG CGAGGCTGGC 
GTTGAAGAAA TCGATCCTAT GGATCTTCTA GACCCCGTTG ACATTCTCTC CAAAATGCCC 
AAGGACTTCT ACGATAAGCT GGAGGAAAAG AAATGGACTC TGCGCAAAGA ATCGTTGGAA 
GTCCTGGAGA AACTGCTCAC TGACCACCCC AAGCTAGAGA ATGGCGAGTA TGGAGCGCTG 
GTAAGCGCTC TTAAAAAGGT TATAACCAAG GACTCCAACG TAGTCCTCGT TGCAATGGCC 
GGAAAATGTC TAGCCTTGCT AGCCAAAGGA TTGGCAAAGC GATTCTCAAA TTACGCATCC 
GCTTGTGTCC CATCCCTGTT GGAAAAATTT AAAGAGAAGA AACCTAATGT TGTGACAGCG 
CTGCGTGAAG CTATTGATGC CATATACGCC TCCACTTCTC TAGAGGCCCA GCAAGAGTCC 
ATTGTAGAGT CCCTGTCCAA CAAAAATCCA AGTGTTAAGT CCGAAACGGC CTTGTTTATT 
GCCCGTGCCC TAACCCGCAC CCAACCAACC GCGCTAAACA AAAAGCTGCT TAAACTTCTG 
ACCACCAGTC TAGTGAAAAC GCTTAACGAA CCAGATCCAA CCGTACGCGA CAGCAGCGCC 
GAAGCTCTTG GTACTTTAAT AAAGCTAATG GGAGACAAAG CGGTGACTCC TTTGCTCGCC 
GACGTCGATC CACTCAAAAT GGCCAAGATA AAGGAGTGCC AAGAAAAGGC GGAGATTAAA 
ATAAAGGTTG CAGGTCCAAA GAAGGAGACA CGACCTGCAT CTGCACCGAC TGCGAAAGCT 
GCTGCTCCGG CTAAGACAGT GGCTGGTAGT GTGGACCCTA AGCCAGTAAC CCGACCGGCC 
ACTACAGGGG CACGTAAGGT CTTGAAAAAA CCGGCGACGG TTAGTGGAGG AGGAGCTACA 
TCTGCCCCAA CTGCAGCTCT TAAAGCGGGT GGAAAACCCC TAGCCACGGA ACGCGAAATA 
ACCCCCGAGG AATTGCAAGA AAAATCCGAA GAAATTCTGC CAGCCGAAAT TCTTAATGGG 
CTGGTGGATT CCAACTGGAA GAACCGCCTT GCTGCTGTGG AACAGCTTCT GGGCGAAATA 
AGCGGTTTTG ATGCAAAACA GGCTGGCATA TCACAGATCC TGATCCGAAC AATCAGTGGA 
CGAAAGCCAG GCCTCAAAGA AATGAATTTC CAGGTACTCA AGTTTAAGCT AGACATAATC 
CGCAGTGTGG CTGAAAACTA TCCACTGACC ACGACAACCG TTGATCTGGT GATTAACGAA 
ATAATCGAGA AGCTAGCAGA CGCCAAGAAT GGTGCAGCAG CTGCAGATGT TTTGTCTGCC 
TTTGCCGAAG CCACTAAACT GGAGTATGTT GTGGGCAAAG TATTGAGCTT TGCTTTTGAA 
CAAAAATCGC CTAAAGTGCA ATCAGAGGCC TTTAACTGGG TTAACAGGTC AATCATAGAA 
TTCGGCTTCC AATTGCAGCC AAAGACACTT ATTGAAGATG TACGAAAAGG CGTGCAGAGC 
ACGAATCCAA CGGTTCGTGC TTCCGCCATT CAAATGGTAG GCACCATGTC TATGTATATG 
GGCAAAGCTC TAATGATGTT CTTCGACAGC GAGAAGCCTG CACTAAAGTC CCAGATCCAG 
GTAGAGTTTG ACAAGAATGT GGGCGAAAAA CCACCCAAGC CTGTTAGAGG AGTTCAACGT 
AGCAGCGGTG GAACCGCTGG TAATTCCCCA GACAATGAGG ACGATGATGG TGGGGCAGCC 
GGAGAAGAGG AGCCCATTAA TATGGCTGAT CTTCTACCAC GCGTTGATAT TGCTCCACAG 
ATTACGGAGG CATTGCTGAA GGAGATGTCC GACAAGGACT GGAAAACTCG CAACGAGGGT 
CTGACTAAGC TGCAGGCTAT TATTTCAGAG GCGCGGCTAA TCAAACCCAG CATTGGCGAC 
TTGGCTCCTG CTCTGGCCCA CCGTCTGGTA GATTCCAATG CTAAGATTGC CCAAACGACA 
CTTGCCATTT GCGAGCAGCT TGCCACAGCC ATGGGGGCTG GTTGTCGAAA TCACGTGCGG 
AACTTGTTCC CTGGCTTTTT ACACGCTCTG GGTGATAACA AGAGTTTTGT AAGAGCCGCT 
GCCCTCAATT GCATCAATAG TTTTGGCGAA AAGGGCGGCT ATAAGGAATT TTTTGAGAGC 
GAAATGATAG CCGATGCCCT GAAAGGAGGA TCCCCAGCTC TAAAGACAGA ATTGTGGGCT 
TGGCTGGCAG ATAAGCTGCC TGGTCTTCCC CCCAAGTCAG TATCGAAGGA GGATATCCAT 
TCAATGGTGC CGCACTTATA TGCTCACATC TGCGATCGAA ACGCCGATGT CCGGAAAAAT 
GCCAACGAGG CGGTGTTGGG CATTATGATT CACCTTGGAT TTGATGCAAT GAACCGCGCT 
CTGGACAAAC AGAAGCCCGC GTCCAAAAAG GATATACTTG CAGCACTCGA AAAAGCGCGT 
CCTAATCTTC CGGTAAAACC GCTGCCCAAA GGCAAGCATC AGGCTCCAAT ACCTGAGGAG 
CCGAAGCTAA AAACAGTGCG CGGCGGTGGA GCAGGTGGTG CGCCTGGAAT CCAGAAGAGT 
GCTACAGCCC GGGTTGCCGG AGGACAAGAC AAGCAGGTAC CAGCGCGCAA AAAGGACGAA 
GATATCGACA CATCGCCGCT ACTGTGCGCC AACAGTGCTA AAAACCAGCG GCTGCTAGAC 
GAGCAAAAAA TGAAGGTACT AAAGTGGACT TTTGTAACCC CAAGAGAGGA ATTCACCGAA 
CTGTTACGCG ACCAAATGAT GACCGCAAAC GTAAATAAAG CACTGATAGC CAACATGTTC 
CACGACGATT TTCGCTACCA CTTGAAAGTC ATCGAGCAGT TGAGCGAAGA TCTGGCTGGT 
AACAGTAAGG CGCTGGTGTG TAATCTGGAC CTAATACTTA AATGGCTGAC GCTGCGATTT 
TACGATACGA ACCCTTCTGT GTTGATTAAG GGTCTCGAAT ATCTAGTACA GGTTTTCCAG 
GTACTCATTG ACGAGGAATA CATTCTGGCC GAGAACGAGG GAAGCAGTTT TGTCCCACAT 
CTGCTTTTAA AGATCGGAGA TCCAAAGGAT GCTGTTCGAA ATGGCGTACG CCGCGTTCTG 
CGGCAAGTAA TATTGGTCTT CCCTTTCGTC AAGGTCTTTG GCTATGTGAT GGAGGGTCTA 
AAATCGAAGA ATGCCCGTCA GCGTACTGAG TGCCTTGACG AGTTAACCTT CCTAATTGAA 
AGCTATGGCA TGAACATCTG TCCCCAATCG GCGGTGCGCG AGATTGCTCG CCAGATTTCC 
GACCGGGATA ATTCCGTACG CAACGCAGCA CTCAACTGCA TCGTCCAGGT CTTCTTTTTG 
TCCGGGGAAA AGACTTACAA GATGATTGGC CATCTTAACG AAAAGGACCT CTCAATGCTA 
GATGAGCGCA TCAAGCGAGC CAAAAAGACT AAGAAGCCCA CGCCACCGCC ATCTGTTGAT 
GTGCCGGCAC CTCAACGACA TGACAGCATC GAAATCGAAG ATGCTGAAGT GGGCAACGGT 
TGCGACGAGC TTCCACCACC AGATGAGGAC GGAACTTTCG ACCAGGCACC TTCGTCACAG 
CTGCTTCTAC TCCAGCAACA GCTTCAACAG TTACAACAGC AGGCCCAGCA ACAGAAGCCC 
AGCGGACCAT TCGGCTTGGA CTCGCAGGTA ATCAGCGAGA TCGAAAAAGA TTGGGTCCGA 
GTGGACCAAA TGGAGCAGAA GCCGCTACTG AACGTAGATA TCTCATCGCT GGACGAGCCT 
ATTAAGGTGA GGCCCACGCG GGCCGGCATT CACTACCCGC AGGAGAAGTT CGATCGGCTG 
ATTTCTCGAC AACACTATAT GCAGCAGACT TTAACGACGT CCCCGTCATC CACGGCTGGA 
ATGACCAGTG GAGTCTCCCC GTATCGCAGT CCTATGCGCT TGCAGCACCA GCAGCCTCAA 
CAGCAGCTGG AGAACAATAT ACCAAATTTG GCGGATGTTT TGCCAAAGCA CGATCCGCAG 
CTGGTAAAAG TTATAAAAGG TGTAAGCAGC ACGGACACTC TAAAGGCGCG CGCGGCTATT 
AACGAGCTTG CCGCCATCAT CGAGGCTCCA GAGAAGCAGG CTGTGCTGCG CGACTATGAA 
GAAATATTTA TTCAGAATGT GCTTGCACAG TTTAAGAATC TCTCACAAAT ACCATCGGCT 
CAATCCGTAG TTGTTTACCA GCCGCTATTA TCCATTCTGT ATACATTTTT CCATGCCAAC 
ATTCTGGGCA AAACGCTGAG CGTGGCCTGC ATCAAAAATC TAATGTCGGC GCTGTTGAAC 
CTTATGGCTG ATCCGAAGTT GGCCGTGGGT GATGATAGTC AGTATAACAA GGTTATTAAT 
GGCATATGTC TAAAAGTGTT GGACAAGGTA GATTTTACGA ATTTAAACTG TGCTTTGATA 
CGCCTATTAC GGGAGACTTG TCCGGAGGCA AAGCTGCCGA AGTTTACAGA CCTGCTGATG 
AAGTGCATAT GGCGCAACGT TAAGATGCTA CCCGAACGAA GCAACGAGCT GAACTATGAC 
GCCGTAATTC TCGAGGTGCA CGAGTTCATG CTCGCGCTGC CTAGCACTTG GTGGCAAAAC 
CGACCGTCGG ACACTCCAAT GCGCACGATT AAGACCATTC TGCATAACAT GGCAAAGGTG 
AAGGGGAATG CTATTCTGCA GCATCTCAAC CAGATTCCAA CACACTCGGA GCTACATACT 
TATTTGATAC GCATTCTCAA GAATTTCCAA AAGGATGGCT CTGCTTCAGG AATTGGTGCC 
TCCCCCCAGA GGGCCAAGGA GATTGCCTCC AAGCGAATTT CGCACCAAAC ACATGATACA 
GTATCACAGA TCTTTAAGCT GATCTCAGAT AGAGATACCA AACAGCAAGG TCTGCAGAAA 
CTTTACGACT TTAAGCAACA AAATCCTGAT ATCGATCTAA GTACCTTTCT GCAAGGCTCC 
AGTGCCCCAT TTCACAAGTA CATCGAGGAG GGTTTAGCCG AGATTGAGAG GAATCAGAAC 
GCTGGATCCA CACAGGATAA TCGGACGGAT GTCAACTACC AGAACAACGG TCCCGATCCC 
GATTTCTGGA TGGACCGCCT GCAGTATCAT ATGACCGGAG GGGCCGCCAA GCTAGCATCT 
GCACGTTCAG CAGACGATGG ATCCCATATG CTGGACAATA AGGTGGTTGA CGAGAACCTC 
TGCCTGAACG GAATGAACGC GCAAAAGGCG TCGCTCATTA AGCGAGAAAA GCGGGACATG 
TCACCAAATC GCTTGCAGCA TCTCCAGGCA AAGCTGGCTC AAATCAAGAA AGAGAATCAT 
GCCCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

msps ( NM_001260200.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247129.1 Drosophila melanogaster mini spindles (msps), transcript variant D, mRNA.

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CloneID	OFa08419
Clone ID Related Accession (Same CDS sequence)	NM_001260200.2
Accession Version	NM_001260200.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mini spindles, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260200.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGAGG ACACAGAGTA CAAGAAGTTG CCGGTGGAGG AGCGGTGTGT CCATAAACTA 
TGGAAAGCCC GCGTCGATGG TTACGAGGAG GCCGCAAAGA TCTTTCGCGA GCTGGATGAC 
GAGAAGTCGC CGGAATGGTC TAAATTTGCA GGGCTCATAA AGAAGATGGT AGTGGACTCT 
AATGCTCTGG CCCAGGAAAA GGGTCTAGAA GCGGCCCTCA TTTTTGTGGA GAACAGCGGG 
CTGGCGGGAC GCACAGTAGG CGACGTGATG ACAGGCATCG TTCAGAAGTG CATTGCGGCG 
CCAAAGACCA AAACCAAGGA GCTCTCCGTC CAAGTGGCTC TAATGTATGT GGAGATAGAG 
AAGCAGGAGG CCGTCGTCGA AGAGCTGGTT AAAGGTATGG AGGCCAAGAA CCCTAAGATT 
GTGTCTGCCT GCGTGGCAGC CACCACACTG GCCCTCCGCG AGTTCGGACA CAAGGTGATC 
GGCGTCAAGC CGTTAATTAA AAAGCTTGCT CCGCTAATGT CTGACCGAGA CAAGACGGTC 
CGAGATGAGG GCAAGCAGCT GGCTGTTGAG ATTTACCGTT GGATCGGAGC TGCAATGAAG 
GCACAGATCT CAACGCTACC ACAGGTAACG CTAAAGGAGC TGGAGGATGA ATTCGACAAA 
CTCAAAGGTG AACGCGTTGA ACCCTCCAGA TATCTAAAGT CCCAGCAGGA AAAGCAAGCG 
AAGATCGCCG ATGCTGCAGC GACCGAGGAT GCATATAATG AGGATGATGG CGAGGCTGGC 
GTTGAAGAAA TCGATCCTAT GGATCTTCTA GACCCCGTTG ACATTCTCTC CAAAATGCCC 
AAGGACTTCT ACGATAAGCT GGAGGAAAAG AAATGGACTC TGCGCAAAGA ATCGTTGGAA 
GTCCTGGAGA AACTGCTCAC TGACCACCCC AAGCTAGAGA ATGGCGAGTA TGGAGCGCTG 
GTAAGCGCTC TTAAAAAGGT TATAACCAAG GACTCCAACG TAGTCCTCGT TGCAATGGCC 
GGAAAATGTC TAGCCTTGCT AGCCAAAGGA TTGGCAAAGC GATTCTCAAA TTACGCATCC 
GCTTGTGTCC CATCCCTGTT GGAAAAATTT AAAGAGAAGA AACCTAATGT TGTGACAGCG 
CTGCGTGAAG CTATTGATGC CATATACGCC TCCACTTCTC TAGAGGCCCA GCAAGAGTCC 
ATTGTAGAGT CCCTGTCCAA CAAAAATCCA AGTGTTAAGT CCGAAACGGC CTTGTTTATT 
GCCCGTGCCC TAACCCGCAC CCAACCAACC GCGCTAAACA AAAAGCTGCT TAAACTTCTG 
ACCACCAGTC TAGTGAAAAC GCTTAACGAA CCAGATCCAA CCGTACGCGA CAGCAGCGCC 
GAAGCTCTTG GTACTTTAAT AAAGCTAATG GGAGACAAAG CGGTGACTCC TTTGCTCGCC 
GACGTCGATC CACTCAAAAT GGCCAAGATA AAGGAGTGCC AAGAAAAGGC GGAGATTAAA 
ATAAAGGTTG CAGGTCCAAA GAAGGAGACA CGACCTGCAT CTGCACCGAC TGCGAAAGCT 
GCTGCTCCGG CTAAGACAGT GGCTGGTAGT GTGGACCCTA AGCCAGTAAC CCGACCGGCC 
ACTACAGGGG CACGTAAGGT CTTGAAAAAA CCGGCGACGG TTAGTGGAGG AGGAGCTACA 
TCTGCCCCAA CTGCAGCTCT TAAAGCGGGT GGAAAACCCC TAGCCACGGA ACGCGAAATA 
ACCCCCGAGG AATTGCAAGA AAAATCCGAA GAAATTCTGC CAGCCGAAAT TCTTAATGGG 
CTGGTGGATT CCAACTGGAA GAACCGCCTT GCTGCTGTGG AACAGCTTCT GGGCGAAATA 
AGCGGTTTTG ATGCAAAACA GGCTGGCATA TCACAGATCC TGATCCGAAC AATCAGTGGA 
CGAAAGCCAG GCCTCAAAGA AATGAATTTC CAGGTACTCA AGTTTAAGCT AGACATAATC 
CGCAGTGTGG CTGAAAACTA TCCACTGACC ACGACAACCG TTGATCTGGT GATTAACGAA 
ATAATCGAGA AGCTAGCAGA CGCCAAGAAT GGTGCAGCAG CTGCAGATGT TTTGTCTGCC 
TTTGCCGAAG CCACTAAACT GGAGTATGTT GTGGGCAAAG TATTGAGCTT TGCTTTTGAA 
CAAAAATCGC CTAAAGTGCA ATCAGAGGCC TTTAACTGGG TTAACAGGTC AATCATAGAA 
TTCGGCTTCC AATTGCAGCC AAAGACACTT ATTGAAGATG TACGAAAAGG CGTGCAGAGC 
ACGAATCCAA CGGTTCGTGC TTCCGCCATT CAAATGGTAG GCACCATGTC TATGTATATG 
GGCAAAGCTC TAATGATGTT CTTCGACAGC GAGAAGCCTG CACTAAAGTC CCAGATCCAG 
GTAGAGTTTG ACAAGAATGT GGGCGAAAAA CCACCCAAGC CTGTTAGAGG AGTTCAACGT 
AGCAGCGGTG GAACCGCTGG TAATTCCCCA GACAATGAGG ACGATGATGG TGGGGCAGCC 
GGAGAAGAGG AGCCCATTAA TATGGCTGAT CTTCTACCAC GCGTTGATAT TGCTCCACAG 
ATTACGGAGG CATTGCTGAA GGAGATGTCC GACAAGGACT GGAAAACTCG CAACGAGGGT 
CTGACTAAGC TGCAGGCTAT TATTTCAGAG GCGCGGCTAA TCAAACCCAG CATTGGCGAC 
TTGGCTCCTG CTCTGGCCCA CCGTCTGGTA GATTCCAATG CTAAGATTGC CCAAACGACA 
CTTGCCATTT GCGAGCAGCT TGCCACAGCC ATGGGGGCTG GTTGTCGAAA TCACGTGCGG 
AACTTGTTCC CTGGCTTTTT ACACGCTCTG GGTGATAACA AGAGTTTTGT AAGAGCCGCT 
GCCCTCAATT GCATCAATAG TTTTGGCGAA AAGGGCGGCT ATAAGGAATT TTTTGAGAGC 
GAAATGATAG CCGATGCCCT GAAAGGAGGA TCCCCAGCTC TAAAGACAGA ATTGTGGGCT 
TGGCTGGCAG ATAAGCTGCC TGGTCTTCCC CCCAAGTCAG TATCGAAGGA GGATATCCAT 
TCAATGGTGC CGCACTTATA TGCTCACATC TGCGATCGAA ACGCCGATGT CCGGAAAAAT 
GCCAACGAGG CGGTGTTGGG CATTATGATT CACCTTGGAT TTGATGCAAT GAACCGCGCT 
CTGGACAAAC AGAAGCCCGC GTCCAAAAAG GATATACTTG CAGCACTCGA AAAAGCGCGT 
CCTAATCTTC CGGTAAAACC GCTGCCCAAA GGCAAGCATC AGGCTCCAAT ACCTGAGGAG 
CCGAAGCTAA AAACAGTGCG CGGCGGTGGA GCAGGTGGTG CGCCTGGAAT CCAGAAGAGT 
GCTACAGCCC GGGTTGCCGG AGGACAAGAC AAGCAGGTAC CAGCGCGCAA AAAGGACGAA 
GATATCGACA CATCGCCGCT ACTGTGCGCC AACAGTGCTA AAAACCAGCG GCTGCTAGAC 
GAGCAAAAAA TGAAGGTACT AAAGTGGACT TTTGTAACCC CAAGAGAGGA ATTCACCGAA 
CTGTTACGCG ACCAAATGAT GACCGCAAAC GTAAATAAAG CACTGATAGC CAACATGTTC 
CACGACGATT TTCGCTACCA CTTGAAAGTC ATCGAGCAGT TGAGCGAAGA TCTGGCTGGT 
AACAGTAAGG CGCTGGTGTG TAATCTGGAC CTAATACTTA AATGGCTGAC GCTGCGATTT 
TACGATACGA ACCCTTCTGT GTTGATTAAG GGTCTCGAAT ATCTAGTACA GGTTTTCCAG 
GTACTCATTG ACGAGGAATA CATTCTGGCC GAGAACGAGG GAAGCAGTTT TGTCCCACAT 
CTGCTTTTAA AGATCGGAGA TCCAAAGGAT GCTGTTCGAA ATGGCGTACG CCGCGTTCTG 
CGGCAAGTAA TATTGGTCTT CCCTTTCGTC AAGGTCTTTG GCTATGTGAT GGAGGGTCTA 
AAATCGAAGA ATGCCCGTCA GCGTACTGAG TGCCTTGACG AGTTAACCTT CCTAATTGAA 
AGCTATGGCA TGAACATCTG TCCCCAATCG GCGGTGCGCG AGATTGCTCG CCAGATTTCC 
GACCGGGATA ATTCCGTACG CAACGCAGCA CTCAACTGCA TCGTCCAGGT CTTCTTTTTG 
TCCGGGGAAA AGACTTACAA GATGATTGGC CATCTTAACG AAAAGGACCT CTCAATGCTA 
GATGAGCGCA TCAAGCGAGC CAAAAAGACT AAGAAGCCCA CGCCACCGCC ATCTGTTGAT 
GTGCCGGCAC CTCAACGACA TGACAGCATC GAAATCGAAG ATGCTGAAGT GGGCAACGGT 
TGCGACGAGC TTCCACCACC AGATGAGGAC GGTTTGCAGC GAGAACTAAA TGCGCGTGGG 
CAGAACAACA TGACAGATCG GAGCGCCACA AACGTGGTTG CCTACTTGAG CTCCCTGACT 
CGACAGGCAA CTTTCGACCA GGCACCTTCG TCACAGCTGC TTCTACTCCA GCAACAGCTT 
CAACAGTTAC AACAGCAGGC CCAGCAACAG AAGCCCAGCG GACCATTCGG CTTGGACTCG 
CAGGTAATCA GCGAGATCGA AAAAGATTGG GTCCGAGTGG ACCAAATGGA GCAGAAGCCG 
CTACTGAACG TAGATATCTC ATCGCTGGAC GAGCCTATTA AGGTGAGGCC CACGCGGGCC 
GGCATTCACT ACCCGCAGGA GAAGTTCGAT CGGCTGATTT CTCGACAACA CTATATGCAG 
CAGACTTTAA CGACGTCCCC GTCATCCACG GCTGGAATGA CCAGTGGAGT CTCCCCGTAT 
CGCAGTCCTA TGCGCTTGCA GCACCAGCAG CCTCAACAGC AGCTGGAGAA CAATATACCA 
AATTTGGCGG ATGTTTTGCC AAAGCACGAT CCGCAGCTGG TAAAAGTTAT AAAAGGTGTA 
AGCAGCACGG ACACTCTAAA GGCGCGCGCG GCTATTAACG AGCTTGCCGC CATCATCGAG 
GCTCCAGAGA AGCAGGCTGT GCTGCGCGAC TATGAAGAAA TATTTATTCA GAATGTGCTT 
GCACAGTTTA AGAATCTCTC ACAAATACCA TCGGCTCAAT CCGTAGTTGT TTACCAGCCG 
CTATTATCCA TTCTGTATAC ATTTTTCCAT GCCAACATTC TGGGCAAAAC GCTGAGCGTG 
GCCTGCATCA AAAATCTAAT GTCGGCGCTG TTGAACCTTA TGGCTGATCC GAAGTTGGCC 
GTGGGTGATG ATAGTCAGTA TAACAAGGTT ATTAATGGCA TATGTCTAAA AGTGTTGGAC 
AAGGTAGATT TTACGAATTT AAACTGTGCT TTGATACGCC TATTACGGGA GACTTGTCCG 
GAGGCAAAGC TGCCGAAGTT TACAGACCTG CTGATGAAGT GCATATGGCG CAACGTTAAG 
ATGCTACCCG AACGAAGCAA CGAGCTGAAC TATGACGCCG TAATTCTCGA GGTGCACGAG 
TTCATGCTCG CGCTGCCTAG CACTTGGTGG CAAAACCGAC CGTCGGACAC TCCAATGCGC 
ACGATTAAGA CCATTCTGCA TAACATGGCA AAGGTGAAGG GGAATGCTAT TCTGCAGCAT 
CTCAACCAGA TTCCAACACA CTCGGAGCTA CATACTTATT TGATACGCAT TCTCAAGAAT 
TTCCAAAAGG ATGGCTCTGC TTCAGGAATT GGTGCCTCCC CCCAGAGGGC CAAGGAGATT 
GCCTCCAAGC GAATTTCGCA CCAAACACAT GATACAGTAT CACAGATCTT TAAGCTGATC 
TCAGATAGAG ATACCAAACA GCAAGGTCTG CAGAAACTTT ACGACTTTAA GCAACAAAAT 
CCTGATATCG ATCTAAGTAC CTTTCTGCAA GGCTCCAGTG CCCCATTTCA CAAGTACATC 
GAGGAGGGTT TAGCCGAGAT TGAGAGGAAT CAGAACGCTG GATCCACACA GGATAATCGG 
ACGGCAGCTA CTCGTTCTTA TCTCACAGAT GTCAACTACC AGAACAACGG TCCCGATCCC 
GATTTCTGGA TGGACCGCCT GCAGTATCAT ATGACCGGAG GGGCCGCCAA GCTAGCATCT 
GCACGTTCAG CAGACGATGG ATCCCATATG CTGGACAATA AGGTGGTTGA CGAGAACCTC 
TGCCTGAACG GAATGAACGC GCAAAAGGCG TCGCTCATTA AGCGAGAAAA GCGGGACATG 
TCACCAAATC GCTTGCAGCA TCTCCAGGCA AAGCTGGCTC AAATCAAGAA AGAGAATCAT 
GCCCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247129.1
CDS	447..6695
Translation	MAEDTEYKKLPVEERCVHKLWKARVDGYEEAAKIFRELDDEKSPEWSKFAGLIKKMVVDSNALAQEKGLEAALIFVENSGLAGRTVGDVMTGIVQKCIAAPKTKTKELSVQVALMYVEIEKQEAVVEELVKGMEAKNPKIVSACVAATTLALREFGHKVIGVKPLIKKLAPLMSDRDKTVRDEGKQLAVEIYRWIGAAMKAQISTLPQVTLKELEDEFDKLKGERVEPSRYLKSQQEKQAKIADAAATEDAYNEDDGEAGVEEIDPMDLLDPVDILSKMPKDFYDKLEEKKWTLRKESLEVLEKLLTDHPKLENGEYGALVSALKKVITKDSNVVLVAMAGKCLALLAKGLAKRFSNYASACVPSLLEKFKEKKPNVVTALREAIDAIYASTSLEAQQESIVESLSNKNPSVKSETALFIARALTRTQPTALNKKLLKLLTTSLVKTLNEPDPTVRDSSAEALGTLIKLMGDKAVTPLLADVDPLKMAKIKECQEKAEIKIKVAGPKKETRPASAPTAKAAAPAKTVAGSVDPKPVTRPATTGARKVLKKPATVSGGGATSAPTAALKAGGKPLATEREITPEELQEKSEEILPAEILNGLVDSNWKNRLAAVEQLLGEISGFDAKQAGISQILIRTISGRKPGLKEMNFQVLKFKLDIIRSVAENYPLTTTTVDLVINEIIEKLADAKNGAAAADVLSAFAEATKLEYVVGKVLSFAFEQKSPKVQSEAFNWVNRSIIEFGFQLQPKTLIEDVRKGVQSTNPTVRASAIQMVGTMSMYMGKALMMFFDSEKPALKSQIQVEFDKNVGEKPPKPVRGVQRSSGGTAGNSPDNEDDDGGAAGEEEPINMADLLPRVDIAPQITEALLKEMSDKDWKTRNEGLTKLQAIISEARLIKPSIGDLAPALAHRLVDSNAKIAQTTLAICEQLATAMGAGCRNHVRNLFPGFLHALGDNKSFVRAAALNCINSFGEKGGYKEFFESEMIADALKGGSPALKTELWAWLADKLPGLPPKSVSKEDIHSMVPHLYAHICDRNADVRKNANEAVLGIMIHLGFDAMNRALDKQKPASKKDILAALEKARPNLPVKPLPKGKHQAPIPEEPKLKTVRGGGAGGAPGIQKSATARVAGGQDKQVPARKKDEDIDTSPLLCANSAKNQRLLDEQKMKVLKWTFVTPREEFTELLRDQMMTANVNKALIANMFHDDFRYHLKVIEQLSEDLAGNSKALVCNLDLILKWLTLRFYDTNPSVLIKGLEYLVQVFQVLIDEEYILAENEGSSFVPHLLLKIGDPKDAVRNGVRRVLRQVILVFPFVKVFGYVMEGLKSKNARQRTECLDELTFLIESYGMNICPQSAVREIARQISDRDNSVRNAALNCIVQVFFLSGEKTYKMIGHLNEKDLSMLDERIKRAKKTKKPTPPPSVDVPAPQRHDSIEIEDAEVGNGCDELPPPDEDGLQRELNARGQNNMTDRSATNVVAYLSSLTRQATFDQAPSSQLLLLQQQLQQLQQQAQQQKPSGPFGLDSQVISEIEKDWVRVDQMEQKPLLNVDISSLDEPIKVRPTRAGIHYPQEKFDRLISRQHYMQQTLTTSPSSTAGMTSGVSPYRSPMRLQHQQPQQQLENNIPNLADVLPKHDPQLVKVIKGVSSTDTLKARAAINELAAIIEAPEKQAVLRDYEEIFIQNVLAQFKNLSQIPSAQSVVVYQPLLSILYTFFHANILGKTLSVACIKNLMSALLNLMADPKLAVGDDSQYNKVINGICLKVLDKVDFTNLNCALIRLLRETCPEAKLPKFTDLLMKCIWRNVKMLPERSNELNYDAVILEVHEFMLALPSTWWQNRPSDTPMRTIKTILHNMAKVKGNAILQHLNQIPTHSELHTYLIRILKNFQKDGSASGIGASPQRAKEIASKRISHQTHDTVSQIFKLISDRDTKQQGLQKLYDFKQQNPDIDLSTFLQGSSAPFHKYIEEGLAEIERNQNAGSTQDNRTAATRSYLTDVNYQNNGPDPDFWMDRLQYHMTGGAAKLASARSADDGSHMLDNKVVDENLCLNGMNAQKASLIKREKRDMSPNRLQHLQAKLAQIKKENHAQ

Target ORF information:

RefSeq Version	NM_001260200.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mini spindles (msps), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260200.2

ORF Insert Sequence:

ATGGCCGAGG ACACAGAGTA CAAGAAGTTG CCGGTGGAGG AGCGGTGTGT CCATAAACTA 
TGGAAAGCCC GCGTCGATGG TTACGAGGAG GCCGCAAAGA TCTTTCGCGA GCTGGATGAC 
GAGAAGTCGC CGGAATGGTC TAAATTTGCA GGGCTCATAA AGAAGATGGT AGTGGACTCT 
AATGCTCTGG CCCAGGAAAA GGGTCTAGAA GCGGCCCTCA TTTTTGTGGA GAACAGCGGG 
CTGGCGGGAC GCACAGTAGG CGACGTGATG ACAGGCATCG TTCAGAAGTG CATTGCGGCG 
CCAAAGACCA AAACCAAGGA GCTCTCCGTC CAAGTGGCTC TAATGTATGT GGAGATAGAG 
AAGCAGGAGG CCGTCGTCGA AGAGCTGGTT AAAGGTATGG AGGCCAAGAA CCCTAAGATT 
GTGTCTGCCT GCGTGGCAGC CACCACACTG GCCCTCCGCG AGTTCGGACA CAAGGTGATC 
GGCGTCAAGC CGTTAATTAA AAAGCTTGCT CCGCTAATGT CTGACCGAGA CAAGACGGTC 
CGAGATGAGG GCAAGCAGCT GGCTGTTGAG ATTTACCGTT GGATCGGAGC TGCAATGAAG 
GCACAGATCT CAACGCTACC ACAGGTAACG CTAAAGGAGC TGGAGGATGA ATTCGACAAA 
CTCAAAGGTG AACGCGTTGA ACCCTCCAGA TATCTAAAGT CCCAGCAGGA AAAGCAAGCG 
AAGATCGCCG ATGCTGCAGC GACCGAGGAT GCATATAATG AGGATGATGG CGAGGCTGGC 
GTTGAAGAAA TCGATCCTAT GGATCTTCTA GACCCCGTTG ACATTCTCTC CAAAATGCCC 
AAGGACTTCT ACGATAAGCT GGAGGAAAAG AAATGGACTC TGCGCAAAGA ATCGTTGGAA 
GTCCTGGAGA AACTGCTCAC TGACCACCCC AAGCTAGAGA ATGGCGAGTA TGGAGCGCTG 
GTAAGCGCTC TTAAAAAGGT TATAACCAAG GACTCCAACG TAGTCCTCGT TGCAATGGCC 
GGAAAATGTC TAGCCTTGCT AGCCAAAGGA TTGGCAAAGC GATTCTCAAA TTACGCATCC 
GCTTGTGTCC CATCCCTGTT GGAAAAATTT AAAGAGAAGA AACCTAATGT TGTGACAGCG 
CTGCGTGAAG CTATTGATGC CATATACGCC TCCACTTCTC TAGAGGCCCA GCAAGAGTCC 
ATTGTAGAGT CCCTGTCCAA CAAAAATCCA AGTGTTAAGT CCGAAACGGC CTTGTTTATT 
GCCCGTGCCC TAACCCGCAC CCAACCAACC GCGCTAAACA AAAAGCTGCT TAAACTTCTG 
ACCACCAGTC TAGTGAAAAC GCTTAACGAA CCAGATCCAA CCGTACGCGA CAGCAGCGCC 
GAAGCTCTTG GTACTTTAAT AAAGCTAATG GGAGACAAAG CGGTGACTCC TTTGCTCGCC 
GACGTCGATC CACTCAAAAT GGCCAAGATA AAGGAGTGCC AAGAAAAGGC GGAGATTAAA 
ATAAAGGTTG CAGGTCCAAA GAAGGAGACA CGACCTGCAT CTGCACCGAC TGCGAAAGCT 
GCTGCTCCGG CTAAGACAGT GGCTGGTAGT GTGGACCCTA AGCCAGTAAC CCGACCGGCC 
ACTACAGGGG CACGTAAGGT CTTGAAAAAA CCGGCGACGG TTAGTGGAGG AGGAGCTACA 
TCTGCCCCAA CTGCAGCTCT TAAAGCGGGT GGAAAACCCC TAGCCACGGA ACGCGAAATA 
ACCCCCGAGG AATTGCAAGA AAAATCCGAA GAAATTCTGC CAGCCGAAAT TCTTAATGGG 
CTGGTGGATT CCAACTGGAA GAACCGCCTT GCTGCTGTGG AACAGCTTCT GGGCGAAATA 
AGCGGTTTTG ATGCAAAACA GGCTGGCATA TCACAGATCC TGATCCGAAC AATCAGTGGA 
CGAAAGCCAG GCCTCAAAGA AATGAATTTC CAGGTACTCA AGTTTAAGCT AGACATAATC 
CGCAGTGTGG CTGAAAACTA TCCACTGACC ACGACAACCG TTGATCTGGT GATTAACGAA 
ATAATCGAGA AGCTAGCAGA CGCCAAGAAT GGTGCAGCAG CTGCAGATGT TTTGTCTGCC 
TTTGCCGAAG CCACTAAACT GGAGTATGTT GTGGGCAAAG TATTGAGCTT TGCTTTTGAA 
CAAAAATCGC CTAAAGTGCA ATCAGAGGCC TTTAACTGGG TTAACAGGTC AATCATAGAA 
TTCGGCTTCC AATTGCAGCC AAAGACACTT ATTGAAGATG TACGAAAAGG CGTGCAGAGC 
ACGAATCCAA CGGTTCGTGC TTCCGCCATT CAAATGGTAG GCACCATGTC TATGTATATG 
GGCAAAGCTC TAATGATGTT CTTCGACAGC GAGAAGCCTG CACTAAAGTC CCAGATCCAG 
GTAGAGTTTG ACAAGAATGT GGGCGAAAAA CCACCCAAGC CTGTTAGAGG AGTTCAACGT 
AGCAGCGGTG GAACCGCTGG TAATTCCCCA GACAATGAGG ACGATGATGG TGGGGCAGCC 
GGAGAAGAGG AGCCCATTAA TATGGCTGAT CTTCTACCAC GCGTTGATAT TGCTCCACAG 
ATTACGGAGG CATTGCTGAA GGAGATGTCC GACAAGGACT GGAAAACTCG CAACGAGGGT 
CTGACTAAGC TGCAGGCTAT TATTTCAGAG GCGCGGCTAA TCAAACCCAG CATTGGCGAC 
TTGGCTCCTG CTCTGGCCCA CCGTCTGGTA GATTCCAATG CTAAGATTGC CCAAACGACA 
CTTGCCATTT GCGAGCAGCT TGCCACAGCC ATGGGGGCTG GTTGTCGAAA TCACGTGCGG 
AACTTGTTCC CTGGCTTTTT ACACGCTCTG GGTGATAACA AGAGTTTTGT AAGAGCCGCT 
GCCCTCAATT GCATCAATAG TTTTGGCGAA AAGGGCGGCT ATAAGGAATT TTTTGAGAGC 
GAAATGATAG CCGATGCCCT GAAAGGAGGA TCCCCAGCTC TAAAGACAGA ATTGTGGGCT 
TGGCTGGCAG ATAAGCTGCC TGGTCTTCCC CCCAAGTCAG TATCGAAGGA GGATATCCAT 
TCAATGGTGC CGCACTTATA TGCTCACATC TGCGATCGAA ACGCCGATGT CCGGAAAAAT 
GCCAACGAGG CGGTGTTGGG CATTATGATT CACCTTGGAT TTGATGCAAT GAACCGCGCT 
CTGGACAAAC AGAAGCCCGC GTCCAAAAAG GATATACTTG CAGCACTCGA AAAAGCGCGT 
CCTAATCTTC CGGTAAAACC GCTGCCCAAA GGCAAGCATC AGGCTCCAAT ACCTGAGGAG 
CCGAAGCTAA AAACAGTGCG CGGCGGTGGA GCAGGTGGTG CGCCTGGAAT CCAGAAGAGT 
GCTACAGCCC GGGTTGCCGG AGGACAAGAC AAGCAGGTAC CAGCGCGCAA AAAGGACGAA 
GATATCGACA CATCGCCGCT ACTGTGCGCC AACAGTGCTA AAAACCAGCG GCTGCTAGAC 
GAGCAAAAAA TGAAGGTACT AAAGTGGACT TTTGTAACCC CAAGAGAGGA ATTCACCGAA 
CTGTTACGCG ACCAAATGAT GACCGCAAAC GTAAATAAAG CACTGATAGC CAACATGTTC 
CACGACGATT TTCGCTACCA CTTGAAAGTC ATCGAGCAGT TGAGCGAAGA TCTGGCTGGT 
AACAGTAAGG CGCTGGTGTG TAATCTGGAC CTAATACTTA AATGGCTGAC GCTGCGATTT 
TACGATACGA ACCCTTCTGT GTTGATTAAG GGTCTCGAAT ATCTAGTACA GGTTTTCCAG 
GTACTCATTG ACGAGGAATA CATTCTGGCC GAGAACGAGG GAAGCAGTTT TGTCCCACAT 
CTGCTTTTAA AGATCGGAGA TCCAAAGGAT GCTGTTCGAA ATGGCGTACG CCGCGTTCTG 
CGGCAAGTAA TATTGGTCTT CCCTTTCGTC AAGGTCTTTG GCTATGTGAT GGAGGGTCTA 
AAATCGAAGA ATGCCCGTCA GCGTACTGAG TGCCTTGACG AGTTAACCTT CCTAATTGAA 
AGCTATGGCA TGAACATCTG TCCCCAATCG GCGGTGCGCG AGATTGCTCG CCAGATTTCC 
GACCGGGATA ATTCCGTACG CAACGCAGCA CTCAACTGCA TCGTCCAGGT CTTCTTTTTG 
TCCGGGGAAA AGACTTACAA GATGATTGGC CATCTTAACG AAAAGGACCT CTCAATGCTA 
GATGAGCGCA TCAAGCGAGC CAAAAAGACT AAGAAGCCCA CGCCACCGCC ATCTGTTGAT 
GTGCCGGCAC CTCAACGACA TGACAGCATC GAAATCGAAG ATGCTGAAGT GGGCAACGGT 
TGCGACGAGC TTCCACCACC AGATGAGGAC GGTTTGCAGC GAGAACTAAA TGCGCGTGGG 
CAGAACAACA TGACAGATCG GAGCGCCACA AACGTGGTTG CCTACTTGAG CTCCCTGACT 
CGACAGGCAA CTTTCGACCA GGCACCTTCG TCACAGCTGC TTCTACTCCA GCAACAGCTT 
CAACAGTTAC AACAGCAGGC CCAGCAACAG AAGCCCAGCG GACCATTCGG CTTGGACTCG 
CAGGTAATCA GCGAGATCGA AAAAGATTGG GTCCGAGTGG ACCAAATGGA GCAGAAGCCG 
CTACTGAACG TAGATATCTC ATCGCTGGAC GAGCCTATTA AGGTGAGGCC CACGCGGGCC 
GGCATTCACT ACCCGCAGGA GAAGTTCGAT CGGCTGATTT CTCGACAACA CTATATGCAG 
CAGACTTTAA CGACGTCCCC GTCATCCACG GCTGGAATGA CCAGTGGAGT CTCCCCGTAT 
CGCAGTCCTA TGCGCTTGCA GCACCAGCAG CCTCAACAGC AGCTGGAGAA CAATATACCA 
AATTTGGCGG ATGTTTTGCC AAAGCACGAT CCGCAGCTGG TAAAAGTTAT AAAAGGTGTA 
AGCAGCACGG ACACTCTAAA GGCGCGCGCG GCTATTAACG AGCTTGCCGC CATCATCGAG 
GCTCCAGAGA AGCAGGCTGT GCTGCGCGAC TATGAAGAAA TATTTATTCA GAATGTGCTT 
GCACAGTTTA AGAATCTCTC ACAAATACCA TCGGCTCAAT CCGTAGTTGT TTACCAGCCG 
CTATTATCCA TTCTGTATAC ATTTTTCCAT GCCAACATTC TGGGCAAAAC GCTGAGCGTG 
GCCTGCATCA AAAATCTAAT GTCGGCGCTG TTGAACCTTA TGGCTGATCC GAAGTTGGCC 
GTGGGTGATG ATAGTCAGTA TAACAAGGTT ATTAATGGCA TATGTCTAAA AGTGTTGGAC 
AAGGTAGATT TTACGAATTT AAACTGTGCT TTGATACGCC TATTACGGGA GACTTGTCCG 
GAGGCAAAGC TGCCGAAGTT TACAGACCTG CTGATGAAGT GCATATGGCG CAACGTTAAG 
ATGCTACCCG AACGAAGCAA CGAGCTGAAC TATGACGCCG TAATTCTCGA GGTGCACGAG 
TTCATGCTCG CGCTGCCTAG CACTTGGTGG CAAAACCGAC CGTCGGACAC TCCAATGCGC 
ACGATTAAGA CCATTCTGCA TAACATGGCA AAGGTGAAGG GGAATGCTAT TCTGCAGCAT 
CTCAACCAGA TTCCAACACA CTCGGAGCTA CATACTTATT TGATACGCAT TCTCAAGAAT 
TTCCAAAAGG ATGGCTCTGC TTCAGGAATT GGTGCCTCCC CCCAGAGGGC CAAGGAGATT 
GCCTCCAAGC GAATTTCGCA CCAAACACAT GATACAGTAT CACAGATCTT TAAGCTGATC 
TCAGATAGAG ATACCAAACA GCAAGGTCTG CAGAAACTTT ACGACTTTAA GCAACAAAAT 
CCTGATATCG ATCTAAGTAC CTTTCTGCAA GGCTCCAGTG CCCCATTTCA CAAGTACATC 
GAGGAGGGTT TAGCCGAGAT TGAGAGGAAT CAGAACGCTG GATCCACACA GGATAATCGG 
ACGGCAGCTA CTCGTTCTTA TCTCACAGAT GTCAACTACC AGAACAACGG TCCCGATCCC 
GATTTCTGGA TGGACCGCCT GCAGTATCAT ATGACCGGAG GGGCCGCCAA GCTAGCATCT 
GCACGTTCAG CAGACGATGG ATCCCATATG CTGGACAATA AGGTGGTTGA CGAGAACCTC 
TGCCTGAACG GAATGAACGC GCAAAAGGCG TCGCTCATTA AGCGAGAAAA GCGGGACATG 
TCACCAAATC GCTTGCAGCA TCTCCAGGCA AAGCTGGCTC AAATCAAGAA AGAGAATCAT 
GCCCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

msps ( NM_001260201.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247130.1 Drosophila melanogaster mini spindles (msps), transcript variant E, mRNA.

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CloneID	OFa08420
Clone ID Related Accession (Same CDS sequence)	NM_001260201.2
Accession Version	NM_001260201.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mini spindles, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260201.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGAGG ACACAGAGTA CAAGAAGTTG CCGGTGGAGG AGCGGTGTGT CCATAAACTA 
TGGAAAGCCC GCGTCGATGG TTACGAGGAG GCCGCAAAGA TCTTTCGCGA GCTGGATGAC 
GAGAAGTCGC CGGAATGGTC TAAATTTGCA GGGCTCATAA AGAAGATGGT AGTGGACTCT 
AATGCTCTGG CCCAGGAAAA GGGTCTAGAA GCGGCCCTCA TTTTTGTGGA GAACAGCGGG 
CTGGCGGGAC GCACAGTAGG CGACGTGATG ACAGGCATCG TTCAGAAGTG CATTGCGGCG 
CCAAAGACCA AAACCAAGGA GCTCTCCGTC CAAGTGGCTC TAATGTATGT GGAGATAGAG 
AAGCAGGAGG CCGTCGTCGA AGAGCTGGTT AAAGGTATGG AGGCCAAGAA CCCTAAGATT 
GTGTCTGCCT GCGTGGCAGC CACCACACTG GCCCTCCGCG AGTTCGGACA CAAGGTGATC 
GGCGTCAAGC CGTTAATTAA AAAGCTTGCT CCGCTAATGT CTGACCGAGA CAAGACGGTC 
CGAGATGAGG GCAAGCAGCT GGCTGTTGAG ATTTACCGTT GGATCGGAGC TGCAATGAAG 
GCACAGATCT CAACGCTACC ACAGGTAACG CTAAAGGAGC TGGAGGATGA ATTCGACAAA 
CTCAAAGGTG AACGCGTTGA ACCCTCCAGA TATCTAAAGT CCCAGCAGGA AAAGCAAGCG 
AAGATCGCCG ATGCTGCAGC GACCGAGGAT GCATATAATG AGGATGATGG CGAGGCTGGC 
GTTGAAGAAA TCGATCCTAT GGATCTTCTA GACCCCGTTG ACATTCTCTC CAAAATGCCC 
AAGGACTTCT ACGATAAGCT GGAGGAAAAG AAATGGACTC TGCGCAAAGA ATCGTTGGAA 
GTCCTGGAGA AACTGCTCAC TGACCACCCC AAGCTAGAGA ATGGCGAGTA TGGAGCGCTG 
GTAAGCGCTC TTAAAAAGGT TATAACCAAG GACTCCAACG TAGTCCTCGT TGCAATGGCC 
GGAAAATGTC TAGCCTTGCT AGCCAAAGGA TTGGCAAAGC GATTCTCAAA TTACGCATCC 
GCTTGTGTCC CATCCCTGTT GGAAAAATTT AAAGAGAAGA AACCTAATGT TGTGACAGCG 
CTGCGTGAAG CTATTGATGC CATATACGCC TCCACTTCTC TAGAGGCCCA GCAAGAGTCC 
ATTGTAGAGT CCCTGTCCAA CAAAAATCCA AGTGTTAAGT CCGAAACGGC CTTGTTTATT 
GCCCGTGCCC TAACCCGCAC CCAACCAACC GCGCTAAACA AAAAGCTGCT TAAACTTCTG 
ACCACCAGTC TAGTGAAAAC GCTTAACGAA CCAGATCCAA CCGTACGCGA CAGCAGCGCC 
GAAGCTCTTG GTACTTTAAT AAAGCTAATG GGAGACAAAG CGGTGACTCC TTTGCTCGCC 
GACGTCGATC CACTCAAAAT GGCCAAGATA AAGGAGTGCC AAGAAAAGGC GGAGATTAAA 
ATAAAGGTTG CAGGTCCAAA GAAGGAGACA CGACCTGCAT CTGCACCGAC TGCGAAAGCT 
GCTGCTCCGG CTAAGACAGT GGCTGGTAGT GTGGACCCTA AGCCAGTAAC CCGACCGGCC 
ACTACAGGGG CACGTAAGGT CTTGAAAAAA CCGGCGACGG TTAGTGGAGG AGGAGCTACA 
TCTGCCCCAA CTGCAGCTCT TAAAGCGGGT GGAAAACCCC TAGCCACGGA ACGCGAAATA 
ACCCCCGAGG AATTGCAAGA AAAATCCGAA GAAATTCTGC CAGCCGAAAT TCTTAATGGG 
CTGGTGGATT CCAACTGGAA GAACCGCCTT GCTGCTGTGG AACAGCTTCT GGGCGAAATA 
AGCGGTTTTG ATGCAAAACA GGCTGGCATA TCACAGATCC TGATCCGAAC AATCAGTGGA 
CGAAAGCCAG GCCTCAAAGA AATGAATTTC CAGGTACTCA AGTTTAAGCT AGACATAATC 
CGCAGTGTGG CTGAAAACTA TCCACTGACC ACGACAACCG TTGATCTGGT GATTAACGAA 
ATAATCGAGA AGCTAGCAGA CGCCAAGAAT GGTGCAGCAG CTGCAGATGT TTTGTCTGCC 
TTTGCCGAAG CCACTAAACT GGAGTATGTT GTGGGCAAAG TATTGAGCTT TGCTTTTGAA 
CAAAAATCGC CTAAAGTGCA ATCAGAGGCC TTTAACTGGG TTAACAGGTC AATCATAGAA 
TTCGGCTTCC AATTGCAGCC AAAGACACTT ATTGAAGATG TACGAAAAGG CGTGCAGAGC 
ACGAATCCAA CGGTTCGTGC TTCCGCCATT CAAATGGTAG GCACCATGTC TATGTATATG 
GGCAAAGCTC TAATGATGTT CTTCGACAGC GAGAAGCCTG CACTAAAGTC CCAGATCCAG 
GTAGAGTTTG ACAAGAATGT GGGCGAAAAA CCACCCAAGC CTGTTAGAGG AGTTCAACGT 
AGCAGCGGTG GAACCGCTGG TAATTCCCCA GACAATGAGG ACGATGATGG TGGGGCAGCC 
GGAGAAGAGG AGCCCATTAA TATGGCTGAT CTTCTACCAC GCGTTGATAT TGCTCCACAG 
ATTACGGAGG CATTGCTGAA GGAGATGTCC GACAAGGACT GGAAAACTCG CAACGAGGGT 
CTGACTAAGC TGCAGGCTAT TATTTCAGAG GCGCGGCTAA TCAAACCCAG CATTGGCGAC 
TTGGCTCCTG CTCTGGCCCA CCGTCTGGTA GATTCCAATG CTAAGATTGC CCAAACGACA 
CTTGCCATTT GCGAGCAGCT TGCCACAGCC ATGGGGGCTG GTTGTCGAAA TCACGTGCGG 
AACTTGTTCC CTGGCTTTTT ACACGCTCTG GGTGATAACA AGAGTTTTGT AAGAGCCGCT 
GCCCTCAATT GCATCAATAG TTTTGGCGAA AAGGGCGGCT ATAAGGAATT TTTTGAGAGC 
GAAATGATAG CCGATGCCCT GAAAGGAGGA TCCCCAGCTC TAAAGACAGA ATTGTGGGCT 
TGGCTGGCAG ATAAGCTGCC TGGTCTTCCC CCCAAGTCAG TATCGAAGGA GGATATCCAT 
TCAATGGTGC CGCACTTATA TGCTCACATC TGCGATCGAA ACGCCGATGT CCGGAAAAAT 
GCCAACGAGG CGGTGTTGGG CATTATGATT CACCTTGGAT TTGATGCAAT GAACCGCGCT 
CTGGACAAAC AGAAGCCCGC GTCCAAAAAG GATATACTTG CAGCACTCGA AAAAGCGCGT 
CCTAATCTTC CGGTAAAACC GCTGCCCAAA GGCAAGCATC AGGCTCCAAT ACCTGAGGAG 
CCGAAGCTAA AAACAGTGCG CGGCGGTGGA GCAGGTGGTG CGCCTGGAAT CCAGAAGAGT 
GCTACAGCCC GGGTTGCCGG AGGACAAGAC AAGCAGGTAC CAGCGCGCAA AAAGGACGAA 
GATATCGACA CATCGCCGCT ACTGTGCGCC AACAGTGCTA AAAACCAGCG GCTGCTAGAC 
GAGCAAAAAA TGAAGGTACT AAAGTGGACT TTTGTAACCC CAAGAGAGGA ATTCACCGAA 
CTGTTACGCG ACCAAATGAT GACCGCAAAC GTAAATAAAG CACTGATAGC CAACATGTTC 
CACGACGATT TTCGCTACCA CTTGAAAGTC ATCGAGCAGT TGAGCGAAGA TCTGGCTGGT 
AACAGTAAGG CGCTGGTGTG TAATCTGGAC CTAATACTTA AATGGCTGAC GCTGCGATTT 
TACGATACGA ACCCTTCTGT GTTGATTAAG GGTCTCGAAT ATCTAGTACA GGTTTTCCAG 
GTACTCATTG ACGAGGAATA CATTCTGGCC GAGAACGAGG GAAGCAGTTT TGTCCCACAT 
CTGCTTTTAA AGATCGGAGA TCCAAAGGAT GCTGTTCGAA ATGGCGTACG CCGCGTTCTG 
CGGCAAGTAA TATTGGTCTT CCCTTTCGTC AAGGTCTTTG GCTATGTGAT GGAGGGTCTA 
AAATCGAAGA ATGCCCGTCA GCGTACTGAG TGCCTTGACG AGTTAACCTT CCTAATTGAA 
AGCTATGGCA TGAACATCTG TCCCCAATCG GCGGTGCGCG AGATTGCTCG CCAGATTTCC 
GACCGGGATA ATTCCGTACG CAACGCAGCA CTCAACTGCA TCGTCCAGGT CTTCTTTTTG 
TCCGGGGAAA AGACTTACAA GATGATTGGC CATCTTAACG AAAAGGACCT CTCAATGCTA 
GATGAGCGCA TCAAGCGAGC CAAAAAGACT AAGAAGCCCA CGCCACCGCC ATCTGTTGAT 
GTGCCGGCAC CTCAACGACA TGACAGCATC GAAATCGAAG ATGCTGAAGT GGGCAACGGT 
TGCGACGAGC TTCCACCACC AGATGAGGAC GGTTTGCAGC GAGAACTAAA TGCGCGTGGG 
CAGAACAACA TGACAGATCG GAGCGCCACA AACGTGGTTG CCTACTTGAG CTCCCTGACT 
CGACAGGCAA CTTTCGACCA GGCACCTTCG TCACAGCTGC TTCTACTCCA GCAACAGCTT 
CAACAGTTAC AACAGCAGGC CCAGCAACAG AAGCCCAGCG GACCATTCGG CTTGGACTCG 
CAGGTAATCA GCGAGATCGA AAAAGATTGG GTCCGAGTGG ACCAAATGGA GCAGAAGCCG 
CTACTGAACG TAGATATCTC ATCGCTGGAC GAGCCTATTA AGGTGAGGCC CACGCGGGCC 
GGCATTCACT ACCCGCAGGA GAAGTTCGAT CGGCTGATTT CTCGACAACA CTATATGCAG 
CAGACTTTAA CGACGTCCCC GTCATCCACG GCTGGAATGA CCAGTGGAGT CTCCCCGTAT 
CGCAGTCCTA TGCGCTTGCA GCACCAGCAG CCTCAACAGC AGCTGGAGAA CAATATACCA 
AATTTGGCGG ATGTTTTGCC AAAGCACGAT CCGCAGCTGG TAAAAGTTAT AAAAGGTGTA 
AGCAGCACGG ACACTCTAAA GGCGCGCGCG GCTATTAACG AGCTTGCCGC CATCATCGAG 
GCTCCAGAGA AGCAGGCTGT GCTGCGCGAC TATGAAGAAA TATTTATTCA GAATGTGCTT 
GCACAGTTTA AGAATCTCTC ACAAATACCA TCGGCTCAAT CCGTAGTTGT TTACCAGCCG 
CTATTATCCA TTCTGTATAC ATTTTTCCAT GCCAACATTC TGGGCAAAAC GCTGAGCGTG 
GCCTGCATCA AAAATCTAAT GTCGGCGCTG TTGAACCTTA TGGCTGATCC GAAGTTGGCC 
GTGGGTGATG ATAGTCAGTA TAACAAGGTT ATTAATGGCA TATGTCTAAA AGTGTTGGAC 
AAGGTAGATT TTACGAATTT AAACTGTGCT TTGATACGCC TATTACGGGA GACTTGTCCG 
GAGGCAAAGC TGCCGAAGTT TACAGACCTG CTGATGAAGT GCATATGGCG CAACGTTAAG 
ATGCTACCCG AACGAAGCAA CGAGCTGAAC TATGACGCCG TAATTCTCGA GGTGCACGAG 
TTCATGCTCG CGCTGCCTAG CACTTGGTGG CAAAACCGAC CGTCGGACAC TCCAATGCGC 
ACGATTAAGA CCATTCTGCA TAACATGGCA AAGGTGAAGG GGAATGCTAT TCTGCAGCAT 
CTCAACCAGA TTCCAACACA CTCGGAGCTA CATACTTATT TGATACGCAT TCTCAAGAAT 
TTCCAAAAGG ATGGCTCTGC TTCAGGAATT GGTGCCTCCC CCCAGAGGGC CAAGGAGATT 
GCCTCCAAGC GAATTTCGCA CCAAACACAT GATACAGTAT CACAGATCTT TAAGCTGATC 
TCAGATAGAG ATACCAAACA GCAAGGTCTG CAGAAACTTT ACGACTTTAA GCAACAAAAT 
CCTGATATCG ATCTAAGTAC CTTTCTGCAA GGCTCCAGTG CCCCATTTCA CAAGTACATC 
GAGGAGGGTT TAGCCGAGAT TGAGAGGAAT CAGAACGCTG GATCCACACA GGATAATCGG 
ACGGATGTCA ACTACCAGAA CAACGGTCCC GATCCCGATT TCTGGATGGA CCGCCTGCAG 
TATCATATGA CCGGAGGGGC CGCCAAGCTA GCATCTGCAC GTTCAGCAGA CGATGGATCC 
CATATGCTGG ACAATAAGGT GGTTGACGAG AACCTCTGCC TGAACGGAAT GAACGCGCAA 
AAGGCGTCGC TCATTAAGCG AGAAAAGCGG GACATGTCAC CAAATCGCTT GCAGCATCTC 
CAGGCAAAGC TGGCTCAAAT CAAGAAAGAG AATCATGCCC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247130.1
CDS	447..6671
Translation	MAEDTEYKKLPVEERCVHKLWKARVDGYEEAAKIFRELDDEKSPEWSKFAGLIKKMVVDSNALAQEKGLEAALIFVENSGLAGRTVGDVMTGIVQKCIAAPKTKTKELSVQVALMYVEIEKQEAVVEELVKGMEAKNPKIVSACVAATTLALREFGHKVIGVKPLIKKLAPLMSDRDKTVRDEGKQLAVEIYRWIGAAMKAQISTLPQVTLKELEDEFDKLKGERVEPSRYLKSQQEKQAKIADAAATEDAYNEDDGEAGVEEIDPMDLLDPVDILSKMPKDFYDKLEEKKWTLRKESLEVLEKLLTDHPKLENGEYGALVSALKKVITKDSNVVLVAMAGKCLALLAKGLAKRFSNYASACVPSLLEKFKEKKPNVVTALREAIDAIYASTSLEAQQESIVESLSNKNPSVKSETALFIARALTRTQPTALNKKLLKLLTTSLVKTLNEPDPTVRDSSAEALGTLIKLMGDKAVTPLLADVDPLKMAKIKECQEKAEIKIKVAGPKKETRPASAPTAKAAAPAKTVAGSVDPKPVTRPATTGARKVLKKPATVSGGGATSAPTAALKAGGKPLATEREITPEELQEKSEEILPAEILNGLVDSNWKNRLAAVEQLLGEISGFDAKQAGISQILIRTISGRKPGLKEMNFQVLKFKLDIIRSVAENYPLTTTTVDLVINEIIEKLADAKNGAAAADVLSAFAEATKLEYVVGKVLSFAFEQKSPKVQSEAFNWVNRSIIEFGFQLQPKTLIEDVRKGVQSTNPTVRASAIQMVGTMSMYMGKALMMFFDSEKPALKSQIQVEFDKNVGEKPPKPVRGVQRSSGGTAGNSPDNEDDDGGAAGEEEPINMADLLPRVDIAPQITEALLKEMSDKDWKTRNEGLTKLQAIISEARLIKPSIGDLAPALAHRLVDSNAKIAQTTLAICEQLATAMGAGCRNHVRNLFPGFLHALGDNKSFVRAAALNCINSFGEKGGYKEFFESEMIADALKGGSPALKTELWAWLADKLPGLPPKSVSKEDIHSMVPHLYAHICDRNADVRKNANEAVLGIMIHLGFDAMNRALDKQKPASKKDILAALEKARPNLPVKPLPKGKHQAPIPEEPKLKTVRGGGAGGAPGIQKSATARVAGGQDKQVPARKKDEDIDTSPLLCANSAKNQRLLDEQKMKVLKWTFVTPREEFTELLRDQMMTANVNKALIANMFHDDFRYHLKVIEQLSEDLAGNSKALVCNLDLILKWLTLRFYDTNPSVLIKGLEYLVQVFQVLIDEEYILAENEGSSFVPHLLLKIGDPKDAVRNGVRRVLRQVILVFPFVKVFGYVMEGLKSKNARQRTECLDELTFLIESYGMNICPQSAVREIARQISDRDNSVRNAALNCIVQVFFLSGEKTYKMIGHLNEKDLSMLDERIKRAKKTKKPTPPPSVDVPAPQRHDSIEIEDAEVGNGCDELPPPDEDGLQRELNARGQNNMTDRSATNVVAYLSSLTRQATFDQAPSSQLLLLQQQLQQLQQQAQQQKPSGPFGLDSQVISEIEKDWVRVDQMEQKPLLNVDISSLDEPIKVRPTRAGIHYPQEKFDRLISRQHYMQQTLTTSPSSTAGMTSGVSPYRSPMRLQHQQPQQQLENNIPNLADVLPKHDPQLVKVIKGVSSTDTLKARAAINELAAIIEAPEKQAVLRDYEEIFIQNVLAQFKNLSQIPSAQSVVVYQPLLSILYTFFHANILGKTLSVACIKNLMSALLNLMADPKLAVGDDSQYNKVINGICLKVLDKVDFTNLNCALIRLLRETCPEAKLPKFTDLLMKCIWRNVKMLPERSNELNYDAVILEVHEFMLALPSTWWQNRPSDTPMRTIKTILHNMAKVKGNAILQHLNQIPTHSELHTYLIRILKNFQKDGSASGIGASPQRAKEIASKRISHQTHDTVSQIFKLISDRDTKQQGLQKLYDFKQQNPDIDLSTFLQGSSAPFHKYIEEGLAEIERNQNAGSTQDNRTDVNYQNNGPDPDFWMDRLQYHMTGGAAKLASARSADDGSHMLDNKVVDENLCLNGMNAQKASLIKREKRDMSPNRLQHLQAKLAQIKKENHAQ

Target ORF information:

RefSeq Version	NM_001260201.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mini spindles (msps), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260201.2

ORF Insert Sequence:

ATGGCCGAGG ACACAGAGTA CAAGAAGTTG CCGGTGGAGG AGCGGTGTGT CCATAAACTA 
TGGAAAGCCC GCGTCGATGG TTACGAGGAG GCCGCAAAGA TCTTTCGCGA GCTGGATGAC 
GAGAAGTCGC CGGAATGGTC TAAATTTGCA GGGCTCATAA AGAAGATGGT AGTGGACTCT 
AATGCTCTGG CCCAGGAAAA GGGTCTAGAA GCGGCCCTCA TTTTTGTGGA GAACAGCGGG 
CTGGCGGGAC GCACAGTAGG CGACGTGATG ACAGGCATCG TTCAGAAGTG CATTGCGGCG 
CCAAAGACCA AAACCAAGGA GCTCTCCGTC CAAGTGGCTC TAATGTATGT GGAGATAGAG 
AAGCAGGAGG CCGTCGTCGA AGAGCTGGTT AAAGGTATGG AGGCCAAGAA CCCTAAGATT 
GTGTCTGCCT GCGTGGCAGC CACCACACTG GCCCTCCGCG AGTTCGGACA CAAGGTGATC 
GGCGTCAAGC CGTTAATTAA AAAGCTTGCT CCGCTAATGT CTGACCGAGA CAAGACGGTC 
CGAGATGAGG GCAAGCAGCT GGCTGTTGAG ATTTACCGTT GGATCGGAGC TGCAATGAAG 
GCACAGATCT CAACGCTACC ACAGGTAACG CTAAAGGAGC TGGAGGATGA ATTCGACAAA 
CTCAAAGGTG AACGCGTTGA ACCCTCCAGA TATCTAAAGT CCCAGCAGGA AAAGCAAGCG 
AAGATCGCCG ATGCTGCAGC GACCGAGGAT GCATATAATG AGGATGATGG CGAGGCTGGC 
GTTGAAGAAA TCGATCCTAT GGATCTTCTA GACCCCGTTG ACATTCTCTC CAAAATGCCC 
AAGGACTTCT ACGATAAGCT GGAGGAAAAG AAATGGACTC TGCGCAAAGA ATCGTTGGAA 
GTCCTGGAGA AACTGCTCAC TGACCACCCC AAGCTAGAGA ATGGCGAGTA TGGAGCGCTG 
GTAAGCGCTC TTAAAAAGGT TATAACCAAG GACTCCAACG TAGTCCTCGT TGCAATGGCC 
GGAAAATGTC TAGCCTTGCT AGCCAAAGGA TTGGCAAAGC GATTCTCAAA TTACGCATCC 
GCTTGTGTCC CATCCCTGTT GGAAAAATTT AAAGAGAAGA AACCTAATGT TGTGACAGCG 
CTGCGTGAAG CTATTGATGC CATATACGCC TCCACTTCTC TAGAGGCCCA GCAAGAGTCC 
ATTGTAGAGT CCCTGTCCAA CAAAAATCCA AGTGTTAAGT CCGAAACGGC CTTGTTTATT 
GCCCGTGCCC TAACCCGCAC CCAACCAACC GCGCTAAACA AAAAGCTGCT TAAACTTCTG 
ACCACCAGTC TAGTGAAAAC GCTTAACGAA CCAGATCCAA CCGTACGCGA CAGCAGCGCC 
GAAGCTCTTG GTACTTTAAT AAAGCTAATG GGAGACAAAG CGGTGACTCC TTTGCTCGCC 
GACGTCGATC CACTCAAAAT GGCCAAGATA AAGGAGTGCC AAGAAAAGGC GGAGATTAAA 
ATAAAGGTTG CAGGTCCAAA GAAGGAGACA CGACCTGCAT CTGCACCGAC TGCGAAAGCT 
GCTGCTCCGG CTAAGACAGT GGCTGGTAGT GTGGACCCTA AGCCAGTAAC CCGACCGGCC 
ACTACAGGGG CACGTAAGGT CTTGAAAAAA CCGGCGACGG TTAGTGGAGG AGGAGCTACA 
TCTGCCCCAA CTGCAGCTCT TAAAGCGGGT GGAAAACCCC TAGCCACGGA ACGCGAAATA 
ACCCCCGAGG AATTGCAAGA AAAATCCGAA GAAATTCTGC CAGCCGAAAT TCTTAATGGG 
CTGGTGGATT CCAACTGGAA GAACCGCCTT GCTGCTGTGG AACAGCTTCT GGGCGAAATA 
AGCGGTTTTG ATGCAAAACA GGCTGGCATA TCACAGATCC TGATCCGAAC AATCAGTGGA 
CGAAAGCCAG GCCTCAAAGA AATGAATTTC CAGGTACTCA AGTTTAAGCT AGACATAATC 
CGCAGTGTGG CTGAAAACTA TCCACTGACC ACGACAACCG TTGATCTGGT GATTAACGAA 
ATAATCGAGA AGCTAGCAGA CGCCAAGAAT GGTGCAGCAG CTGCAGATGT TTTGTCTGCC 
TTTGCCGAAG CCACTAAACT GGAGTATGTT GTGGGCAAAG TATTGAGCTT TGCTTTTGAA 
CAAAAATCGC CTAAAGTGCA ATCAGAGGCC TTTAACTGGG TTAACAGGTC AATCATAGAA 
TTCGGCTTCC AATTGCAGCC AAAGACACTT ATTGAAGATG TACGAAAAGG CGTGCAGAGC 
ACGAATCCAA CGGTTCGTGC TTCCGCCATT CAAATGGTAG GCACCATGTC TATGTATATG 
GGCAAAGCTC TAATGATGTT CTTCGACAGC GAGAAGCCTG CACTAAAGTC CCAGATCCAG 
GTAGAGTTTG ACAAGAATGT GGGCGAAAAA CCACCCAAGC CTGTTAGAGG AGTTCAACGT 
AGCAGCGGTG GAACCGCTGG TAATTCCCCA GACAATGAGG ACGATGATGG TGGGGCAGCC 
GGAGAAGAGG AGCCCATTAA TATGGCTGAT CTTCTACCAC GCGTTGATAT TGCTCCACAG 
ATTACGGAGG CATTGCTGAA GGAGATGTCC GACAAGGACT GGAAAACTCG CAACGAGGGT 
CTGACTAAGC TGCAGGCTAT TATTTCAGAG GCGCGGCTAA TCAAACCCAG CATTGGCGAC 
TTGGCTCCTG CTCTGGCCCA CCGTCTGGTA GATTCCAATG CTAAGATTGC CCAAACGACA 
CTTGCCATTT GCGAGCAGCT TGCCACAGCC ATGGGGGCTG GTTGTCGAAA TCACGTGCGG 
AACTTGTTCC CTGGCTTTTT ACACGCTCTG GGTGATAACA AGAGTTTTGT AAGAGCCGCT 
GCCCTCAATT GCATCAATAG TTTTGGCGAA AAGGGCGGCT ATAAGGAATT TTTTGAGAGC 
GAAATGATAG CCGATGCCCT GAAAGGAGGA TCCCCAGCTC TAAAGACAGA ATTGTGGGCT 
TGGCTGGCAG ATAAGCTGCC TGGTCTTCCC CCCAAGTCAG TATCGAAGGA GGATATCCAT 
TCAATGGTGC CGCACTTATA TGCTCACATC TGCGATCGAA ACGCCGATGT CCGGAAAAAT 
GCCAACGAGG CGGTGTTGGG CATTATGATT CACCTTGGAT TTGATGCAAT GAACCGCGCT 
CTGGACAAAC AGAAGCCCGC GTCCAAAAAG GATATACTTG CAGCACTCGA AAAAGCGCGT 
CCTAATCTTC CGGTAAAACC GCTGCCCAAA GGCAAGCATC AGGCTCCAAT ACCTGAGGAG 
CCGAAGCTAA AAACAGTGCG CGGCGGTGGA GCAGGTGGTG CGCCTGGAAT CCAGAAGAGT 
GCTACAGCCC GGGTTGCCGG AGGACAAGAC AAGCAGGTAC CAGCGCGCAA AAAGGACGAA 
GATATCGACA CATCGCCGCT ACTGTGCGCC AACAGTGCTA AAAACCAGCG GCTGCTAGAC 
GAGCAAAAAA TGAAGGTACT AAAGTGGACT TTTGTAACCC CAAGAGAGGA ATTCACCGAA 
CTGTTACGCG ACCAAATGAT GACCGCAAAC GTAAATAAAG CACTGATAGC CAACATGTTC 
CACGACGATT TTCGCTACCA CTTGAAAGTC ATCGAGCAGT TGAGCGAAGA TCTGGCTGGT 
AACAGTAAGG CGCTGGTGTG TAATCTGGAC CTAATACTTA AATGGCTGAC GCTGCGATTT 
TACGATACGA ACCCTTCTGT GTTGATTAAG GGTCTCGAAT ATCTAGTACA GGTTTTCCAG 
GTACTCATTG ACGAGGAATA CATTCTGGCC GAGAACGAGG GAAGCAGTTT TGTCCCACAT 
CTGCTTTTAA AGATCGGAGA TCCAAAGGAT GCTGTTCGAA ATGGCGTACG CCGCGTTCTG 
CGGCAAGTAA TATTGGTCTT CCCTTTCGTC AAGGTCTTTG GCTATGTGAT GGAGGGTCTA 
AAATCGAAGA ATGCCCGTCA GCGTACTGAG TGCCTTGACG AGTTAACCTT CCTAATTGAA 
AGCTATGGCA TGAACATCTG TCCCCAATCG GCGGTGCGCG AGATTGCTCG CCAGATTTCC 
GACCGGGATA ATTCCGTACG CAACGCAGCA CTCAACTGCA TCGTCCAGGT CTTCTTTTTG 
TCCGGGGAAA AGACTTACAA GATGATTGGC CATCTTAACG AAAAGGACCT CTCAATGCTA 
GATGAGCGCA TCAAGCGAGC CAAAAAGACT AAGAAGCCCA CGCCACCGCC ATCTGTTGAT 
GTGCCGGCAC CTCAACGACA TGACAGCATC GAAATCGAAG ATGCTGAAGT GGGCAACGGT 
TGCGACGAGC TTCCACCACC AGATGAGGAC GGTTTGCAGC GAGAACTAAA TGCGCGTGGG 
CAGAACAACA TGACAGATCG GAGCGCCACA AACGTGGTTG CCTACTTGAG CTCCCTGACT 
CGACAGGCAA CTTTCGACCA GGCACCTTCG TCACAGCTGC TTCTACTCCA GCAACAGCTT 
CAACAGTTAC AACAGCAGGC CCAGCAACAG AAGCCCAGCG GACCATTCGG CTTGGACTCG 
CAGGTAATCA GCGAGATCGA AAAAGATTGG GTCCGAGTGG ACCAAATGGA GCAGAAGCCG 
CTACTGAACG TAGATATCTC ATCGCTGGAC GAGCCTATTA AGGTGAGGCC CACGCGGGCC 
GGCATTCACT ACCCGCAGGA GAAGTTCGAT CGGCTGATTT CTCGACAACA CTATATGCAG 
CAGACTTTAA CGACGTCCCC GTCATCCACG GCTGGAATGA CCAGTGGAGT CTCCCCGTAT 
CGCAGTCCTA TGCGCTTGCA GCACCAGCAG CCTCAACAGC AGCTGGAGAA CAATATACCA 
AATTTGGCGG ATGTTTTGCC AAAGCACGAT CCGCAGCTGG TAAAAGTTAT AAAAGGTGTA 
AGCAGCACGG ACACTCTAAA GGCGCGCGCG GCTATTAACG AGCTTGCCGC CATCATCGAG 
GCTCCAGAGA AGCAGGCTGT GCTGCGCGAC TATGAAGAAA TATTTATTCA GAATGTGCTT 
GCACAGTTTA AGAATCTCTC ACAAATACCA TCGGCTCAAT CCGTAGTTGT TTACCAGCCG 
CTATTATCCA TTCTGTATAC ATTTTTCCAT GCCAACATTC TGGGCAAAAC GCTGAGCGTG 
GCCTGCATCA AAAATCTAAT GTCGGCGCTG TTGAACCTTA TGGCTGATCC GAAGTTGGCC 
GTGGGTGATG ATAGTCAGTA TAACAAGGTT ATTAATGGCA TATGTCTAAA AGTGTTGGAC 
AAGGTAGATT TTACGAATTT AAACTGTGCT TTGATACGCC TATTACGGGA GACTTGTCCG 
GAGGCAAAGC TGCCGAAGTT TACAGACCTG CTGATGAAGT GCATATGGCG CAACGTTAAG 
ATGCTACCCG AACGAAGCAA CGAGCTGAAC TATGACGCCG TAATTCTCGA GGTGCACGAG 
TTCATGCTCG CGCTGCCTAG CACTTGGTGG CAAAACCGAC CGTCGGACAC TCCAATGCGC 
ACGATTAAGA CCATTCTGCA TAACATGGCA AAGGTGAAGG GGAATGCTAT TCTGCAGCAT 
CTCAACCAGA TTCCAACACA CTCGGAGCTA CATACTTATT TGATACGCAT TCTCAAGAAT 
TTCCAAAAGG ATGGCTCTGC TTCAGGAATT GGTGCCTCCC CCCAGAGGGC CAAGGAGATT 
GCCTCCAAGC GAATTTCGCA CCAAACACAT GATACAGTAT CACAGATCTT TAAGCTGATC 
TCAGATAGAG ATACCAAACA GCAAGGTCTG CAGAAACTTT ACGACTTTAA GCAACAAAAT 
CCTGATATCG ATCTAAGTAC CTTTCTGCAA GGCTCCAGTG CCCCATTTCA CAAGTACATC 
GAGGAGGGTT TAGCCGAGAT TGAGAGGAAT CAGAACGCTG GATCCACACA GGATAATCGG 
ACGGATGTCA ACTACCAGAA CAACGGTCCC GATCCCGATT TCTGGATGGA CCGCCTGCAG 
TATCATATGA CCGGAGGGGC CGCCAAGCTA GCATCTGCAC GTTCAGCAGA CGATGGATCC 
CATATGCTGG ACAATAAGGT GGTTGACGAG AACCTCTGCC TGAACGGAAT GAACGCGCAA 
AAGGCGTCGC TCATTAAGCG AGAAAAGCGG GACATGTCAC CAAATCGCTT GCAGCATCTC 
CAGGCAAAGC TGGCTCAAAT CAAGAAAGAG AATCATGCCC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

msps ( NM_001260202.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247131.1 Drosophila melanogaster mini spindles (msps), transcript variant F, mRNA.

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CloneID	OFa08421
Clone ID Related Accession (Same CDS sequence)	NM_001260202.1 , NM_001170156.2
Accession Version	NM_001260202.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mini spindles, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGAGG ACACAGAGTA CAAGAAGTTG CCGGTGGAGG AGCGGTGTGT CCATAAACTA 
TGGAAAGCCC GCGTCGATGG TTACGAGGAG GCCGCAAAGA TCTTTCGCGA GCTGGATGAC 
GAGAAGTCGC CGGAATGGTC TAAATTTGCA GGGCTCATAA AGAAGATGGT AGTGGACTCT 
AATGCTCTGG CCCAGGAAAA GGGTCTAGAA GCGGCCCTCA TTTTTGTGGA GAACAGCGGG 
CTGGCGGGAC GCACAGTAGG CGACGTGATG ACAGGCATCG TTCAGAAGTG CATTGCGGCG 
CCAAAGACCA AAACCAAGGA GCTCTCCGTC CAAGTGGCTC TAATGTATGT GGAGATAGAG 
AAGCAGGAGG CCGTCGTCGA AGAGCTGGTT AAAGGTATGG AGGCCAAGAA CCCTAAGATT 
GTGTCTGCCT GCGTGGCAGC CACCACACTG GCCCTCCGCG AGTTCGGACA CAAGGTGATC 
GGCGTCAAGC CGTTAATTAA AAAGCTTGCT CCGCTAATGT CTGACCGAGA CAAGACGGTC 
CGAGATGAGG GCAAGCAGCT GGCTGTTGAG ATTTACCGTT GGATCGGAGC TGCAATGAAG 
GCACAGATCT CAACGCTACC ACAGGTAACG CTAAAGGAGC TGGAGGATGA ATTCGACAAA 
CTCAAAGGTG AACGCGTTGA ACCCTCCAGA TATCTAAAGT CCCAGCAGGA AAAGCAAGCG 
AAGATCGCCG ATGCTGCAGC GACCGAGGAT GCATATAATG AGGATGATGG CGAGGCTGGC 
GTTGAAGAAA TCGATCCTAT GGATCTTCTA GACCCCGTTG ACATTCTCTC CAAAATGCCC 
AAGGACTTCT ACGATAAGCT GGAGGAAAAG AAATGGACTC TGCGCAAAGA ATCGTTGGAA 
GTCCTGGAGA AACTGCTCAC TGACCACCCC AAGCTAGAGA ATGGCGAGTA TGGAGCGCTG 
GTAAGCGCTC TTAAAAAGGT TATAACCAAG GACTCCAACG TAGTCCTCGT TGCAATGGCC 
GGAAAATGTC TAGCCTTGCT AGCCAAAGGA TTGGCAAAGC GATTCTCAAA TTACGCATCC 
GCTTGTGTCC CATCCCTGTT GGAAAAATTT AAAGAGAAGA AACCTAATGT TGTGACAGCG 
CTGCGTGAAG CTATTGATGC CATATACGCC TCCACTTCTC TAGAGGCCCA GCAAGAGTCC 
ATTGTAGAGT CCCTGTCCAA CAAAAATCCA AGTGTTAAGT CCGAAACGGC CTTGTTTATT 
GCCCGTGCCC TAACCCGCAC CCAACCAACC GCGCTAAACA AAAAGCTGCT TAAACTTCTG 
ACCACCAGTC TAGTGAAAAC GCTTAACGAA CCAGATCCAA CCGTACGCGA CAGCAGCGCC 
GAAGCTCTTG GTACTTTAAT AAAGCTAATG GGAGACAAAG CGGTGACTCC TTTGCTCGCC 
GACGTCGATC CACTCAAAAT GGCCAAGATA AAGGAGTGCC AAGAAAAGGC GGAGATTAAA 
ATAAAGGTTG CAGGTCCAAA GAAGGAGACA CGACCTGCAT CTGCACCGAC TGCGAAAGCT 
GCTGCTCCGG CTAAGACAGT GGCTGGTAGT GTGGACCCTA AGCCAGTAAC CCGACCGGCC 
ACTACAGGGG CACGTAAGGT CTTGAAAAAA CCGGCGACGG TTAGTGGAGG AGGAGCTACA 
TCTGCCCCAA CTGCAGCTCT TAAAGCGGGT GGAAAACCCC TAGCCACGGA ACGCGAAATA 
ACCCCCGAGG AATTGCAAGA AAAATCCGAA GAAATTCTGC CAGCCGAAAT TCTTAATGGG 
CTGGTGGATT CCAACTGGAA GAACCGCCTT GCTGCTGTGG AACAGCTTCT GGGCGAAATA 
AGCGGTTTTG ATGCAAAACA GGCTGGCATA TCACAGATCC TGATCCGAAC AATCAGTGGA 
CGAAAGCCAG GCCTCAAAGA AATGAATTTC CAGGTACTCA AGTTTAAGCT AGACATAATC 
CGCAGTGTGG CTGAAAACTA TCCACTGACC ACGACAACCG TTGATCTGGT GATTAACGAA 
ATAATCGAGA AGCTAGCAGA CGCCAAGAAT GGTGCAGCAG CTGCAGATGT TTTGTCTGCC 
TTTGCCGAAG CCACTAAACT GGAGTATGTT GTGGGCAAAG TATTGAGCTT TGCTTTTGAA 
CAAAAATCGC CTAAAGTGCA ATCAGAGGCC TTTAACTGGG TTAACAGGTC AATCATAGAA 
TTCGGCTTCC AATTGCAGCC AAAGACACTT ATTGAAGATG TACGAAAAGG CGTGCAGAGC 
ACGAATCCAA CGGTTCGTGC TTCCGCCATT CAAATGGTAG GCACCATGTC TATGTATATG 
GGCAAAGCTC TAATGATGTT CTTCGACAGC GAGAAGCCTG CACTAAAGTC CCAGATCCAG 
GTAGAGTTTG ACAAGAATGT GGGCGAAAAA CCACCCAAGC CTGTTAGAGG AGTTCAACGT 
AGCAGCGGTG GAACCGCTGG TAATTCCCCA GACAATGAGG ACGATGATGG TGGGGCAGCC 
GGAGAAGAGG AGCCCATTAA TATGGCTGAT CTTCTACCAC GCGTTGATAT TGCTCCACAG 
ATTACGGAGG CATTGCTGAA GGAGATGTCC GACAAGGACT GGAAAACTCG CAACGAGGGT 
CTGACTAAGC TGCAGGCTAT TATTTCAGAG GCGCGGCTAA TCAAACCCAG CATTGGCGAC 
TTGGCTCCTG CTCTGGCCCA CCGTCTGGTA GATTCCAATG CTAAGATTGC CCAAACGACA 
CTTGCCATTT GCGAGCAGCT TGCCACAGCC ATGGGGGCTG GTTGTCGAAA TCACGTGCGG 
AACTTGTTCC CTGGCTTTTT ACACGCTCTG GGTGATAACA AGAGTTTTGT AAGAGCCGCT 
GCCCTCAATT GCATCAATAG TTTTGGCGAA AAGGGCGGCT ATAAGGAATT TTTTGAGAGC 
GAAATGATAG CCGATGCCCT GAAAGGAGGA TCCCCAGCTC TAAAGACAGA ATTGTGGGCT 
TGGCTGGCAG ATAAGCTGCC TGGTCTTCCC CCCAAGTCAG TATCGAAGGA GGATATCCAT 
TCAATGGTGC CGCACTTATA TGCTCACATC TGCGATCGAA ACGCCGATGT CCGGAAAAAT 
GCCAACGAGG CGGTGTTGGG CATTATGATT CACCTTGGAT TTGATGCAAT GAACCGCGCT 
CTGGACAAAC AGAAGCCCGC GTCCAAAAAG GATATACTTG CAGCACTCGA AAAAGCGCGT 
CCTAATCTTC CGGTAAAACC GCTGCCCAAA GGCAAGCATC AGGCTCCAAT ACCTGAGGAG 
CCGAAGCTAA AAACAGTGCG CGGCGGTGGA GCAGGTGGTG CGCCTGGAAT CCAGAAGAGT 
GCTACAGCCC GGGTTGCCGG AGGACAAGAC AAGCAGGTAC CAGCGCGCAA AAAGGACGAA 
GATATCGACA CATCGCCGCT ACTGTGCGCC AACAGTGCTA AAAACCAGCG GCTGCTAGAC 
GAGCAAAAAA TGAAGGTACT AAAGTGGACT TTTGTAACCC CAAGAGAGGA ATTCACCGAA 
CTGTTACGCG ACCAAATGAT GACCGCAAAC GTAAATAAAG CACTGATAGC CAACATGTTC 
CACGACGATT TTCGCTACCA CTTGAAAGTC ATCGAGCAGT TGAGCGAAGA TCTGGCTGGT 
AACAGTAAGG CGCTGGTGTG TAATCTGGAC CTAATACTTA AATGGCTGAC GCTGCGATTT 
TACGATACGA ACCCTTCTGT GTTGATTAAG GGTCTCGAAT ATCTAGTACA GGTTTTCCAG 
GTACTCATTG ACGAGGAATA CATTCTGGCC GAGAACGAGG GAAGCAGTTT TGTCCCACAT 
CTGCTTTTAA AGATCGGAGA TCCAAAGGAT GCTGTTCGAA ATGGCGTACG CCGCGTTCTG 
CGGCAAGTAA TATTGGTCTT CCCTTTCGTC AAGGTCTTTG GCTATGTGAT GGAGGGTCTA 
AAATCGAAGA ATGCCCGTCA GCGTACTGAG TGCCTTGACG AGTTAACCTT CCTAATTGAA 
AGCTATGGCA TGAACATCTG TCCCCAATCG GCGGTGCGCG AGATTGCTCG CCAGATTTCC 
GACCGGGATA ATTCCGTACG CAACGCAGCA CTCAACTGCA TCGTCCAGGT CTTCTTTTTG 
TCCGGGGAAA AGACTTACAA GATGATTGGC CATCTTAACG AAAAGGACCT CTCAATGCTA 
GATGAGCGCA TCAAGCGAGC CAAAAAGACT AAGAAGCCCA CGCCACCGCC ATCTGTTGAT 
GTGCCGGCAC CTCAACGACA TGACAGCATC GAAATCGAAG ATGCTGAAGT GGGCAACGGT 
TGCGACGAGC TTCCACCACC AGATGAGGAC GGAACTTTCG ACCAGGCACC TTCGTCACAG 
CTGCTTCTAC TCCAGCAACA GCTTCAACAG TTACAACAGC AGGCCCAGCA ACAGAAGCCC 
AGCGGACCAT TCGGCTTGGA CTCGCAGGTA ATCAGCGAGA TCGAAAAAGA TTGGGTCCGA 
GTGGACCAAA TGGAGCAGAA GCCGCTACTG AACGTAGATA TCTCATCGCT GGACGAGCCT 
ATTAAGGTGA GGCCCACGCG GGCCGGCATT CACTACCCGC AGGAGAAGTT CGATCGGCTG 
ATTTCTCGAC AACACTATAT GCAGCAGACT TTAACGACGT CCCCGTCATC CACGGCTGGA 
ATGACCAGTG GAGTCTCCCC GTATCGCAGT CCTATGCGCT TGCAGCACCA GCAGCCTCAA 
CAGCAGCTGG AGAACAATAT ACCAAATTTG GCGGATGTTT TGCCAAAGCA CGATCCGCAG 
CTGGTAAAAG TTATAAAAGG TGTAAGCAGC ACGGACACTC TAAAGGCGCG CGCGGCTATT 
AACGAGCTTG CCGCCATCAT CGAGGCTCCA GAGAAGCAGG CTGTGCTGCG CGACTATGAA 
GAAATATTTA TTCAGAATGT GCTTGCACAG TTTAAGAATC TCTCACAAAT ACCATCGGCT 
CAATCCGTAG TTGTTTACCA GCCGCTATTA TCCATTCTGT ATACATTTTT CCATGCCAAC 
ATTCTGGGCA AAACGCTGAG CGTGGCCTGC ATCAAAAATC TAATGTCGGC GCTGTTGAAC 
CTTATGGCTG ATCCGAAGTT GGCCGTGGGT GATGATAGTC AGTATAACAA GGTTATTAAT 
GGCATATGTC TAAAAGTGTT GGACAAGGTA GATTTTACGA ATTTAAACTG TGCTTTGATA 
CGCCTATTAC GGGAGACTTG TCCGGAGGCA AAGCTGCCGA AGTTTACAGA CCTGCTGATG 
AAGTGCATAT GGCGCAACGT TAAGATGCTA CCCGAACGAA GCAACGAGCT GAACTATGAC 
GCCGTAATTC TCGAGGTGCA CGAGTTCATG CTCGCGCTGC CTAGCACTTG GTGGCAAAAC 
CGACCGTCGG ACACTCCAAT GCGCACGATT AAGACCATTC TGCATAACAT GGCAAAGGTG 
AAGGGGAATG CTATTCTGCA GCATCTCAAC CAGATTCCAA CACACTCGGA GCTACATACT 
TATTTGATAC GCATTCTCAA GAATTTCCAA AAGGATGGCT CTGCTTCAGG AATTGGTGCC 
TCCCCCCAGA GGGCCAAGGA GATTGCCTCC AAGCGAATTT CGCACCAAAC ACATGATACA 
GTATCACAGA TCTTTAAGCT GATCTCAGAT AGAGATACCA AACAGCAAGG TCTGCAGAAA 
CTTTACGACT TTAAGCAACA AAATCCTGAT ATCGATCTAA GTACCTTTCT GCAAGGCTCC 
AGTGCCCCAT TTCACAAGTA CATCGAGGAG GGTTTAGCCG AGATTGAGAG GAATCAGAAC 
GCTGGATCCA CACAGGATAA TCGGACGGCA GCTACTCGTT CTTATCTCAC AGATGTCAAC 
TACCAGAACA ACGGTCCCGA TCCCGATTTC TGGATGGACC GCCTGCAGTA TCATATGACC 
GGAGGGGCCG CCAAGCTAGC ATCTGCACGT TCAGCAGACG ATGGATCCCA TATGCTGGAC 
AATAAGGTGG TTGACGAGAA CCTCTGCCTG AACGGAATGA ACGCGCAAAA GGCGTCGCTC 
ATTAAGCGAG AAAAGCGGGA CATGTCACCA AATCGCTTGC AGCATCTCCA GGCAAAGCTG 
GCTCAAATCA AGAAAGAGAA TCATGCCCAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247131.1
CDS	110..6262
Translation	MAEDTEYKKLPVEERCVHKLWKARVDGYEEAAKIFRELDDEKSPEWSKFAGLIKKMVVDSNALAQEKGLEAALIFVENSGLAGRTVGDVMTGIVQKCIAAPKTKTKELSVQVALMYVEIEKQEAVVEELVKGMEAKNPKIVSACVAATTLALREFGHKVIGVKPLIKKLAPLMSDRDKTVRDEGKQLAVEIYRWIGAAMKAQISTLPQVTLKELEDEFDKLKGERVEPSRYLKSQQEKQAKIADAAATEDAYNEDDGEAGVEEIDPMDLLDPVDILSKMPKDFYDKLEEKKWTLRKESLEVLEKLLTDHPKLENGEYGALVSALKKVITKDSNVVLVAMAGKCLALLAKGLAKRFSNYASACVPSLLEKFKEKKPNVVTALREAIDAIYASTSLEAQQESIVESLSNKNPSVKSETALFIARALTRTQPTALNKKLLKLLTTSLVKTLNEPDPTVRDSSAEALGTLIKLMGDKAVTPLLADVDPLKMAKIKECQEKAEIKIKVAGPKKETRPASAPTAKAAAPAKTVAGSVDPKPVTRPATTGARKVLKKPATVSGGGATSAPTAALKAGGKPLATEREITPEELQEKSEEILPAEILNGLVDSNWKNRLAAVEQLLGEISGFDAKQAGISQILIRTISGRKPGLKEMNFQVLKFKLDIIRSVAENYPLTTTTVDLVINEIIEKLADAKNGAAAADVLSAFAEATKLEYVVGKVLSFAFEQKSPKVQSEAFNWVNRSIIEFGFQLQPKTLIEDVRKGVQSTNPTVRASAIQMVGTMSMYMGKALMMFFDSEKPALKSQIQVEFDKNVGEKPPKPVRGVQRSSGGTAGNSPDNEDDDGGAAGEEEPINMADLLPRVDIAPQITEALLKEMSDKDWKTRNEGLTKLQAIISEARLIKPSIGDLAPALAHRLVDSNAKIAQTTLAICEQLATAMGAGCRNHVRNLFPGFLHALGDNKSFVRAAALNCINSFGEKGGYKEFFESEMIADALKGGSPALKTELWAWLADKLPGLPPKSVSKEDIHSMVPHLYAHICDRNADVRKNANEAVLGIMIHLGFDAMNRALDKQKPASKKDILAALEKARPNLPVKPLPKGKHQAPIPEEPKLKTVRGGGAGGAPGIQKSATARVAGGQDKQVPARKKDEDIDTSPLLCANSAKNQRLLDEQKMKVLKWTFVTPREEFTELLRDQMMTANVNKALIANMFHDDFRYHLKVIEQLSEDLAGNSKALVCNLDLILKWLTLRFYDTNPSVLIKGLEYLVQVFQVLIDEEYILAENEGSSFVPHLLLKIGDPKDAVRNGVRRVLRQVILVFPFVKVFGYVMEGLKSKNARQRTECLDELTFLIESYGMNICPQSAVREIARQISDRDNSVRNAALNCIVQVFFLSGEKTYKMIGHLNEKDLSMLDERIKRAKKTKKPTPPPSVDVPAPQRHDSIEIEDAEVGNGCDELPPPDEDGTFDQAPSSQLLLLQQQLQQLQQQAQQQKPSGPFGLDSQVISEIEKDWVRVDQMEQKPLLNVDISSLDEPIKVRPTRAGIHYPQEKFDRLISRQHYMQQTLTTSPSSTAGMTSGVSPYRSPMRLQHQQPQQQLENNIPNLADVLPKHDPQLVKVIKGVSSTDTLKARAAINELAAIIEAPEKQAVLRDYEEIFIQNVLAQFKNLSQIPSAQSVVVYQPLLSILYTFFHANILGKTLSVACIKNLMSALLNLMADPKLAVGDDSQYNKVINGICLKVLDKVDFTNLNCALIRLLRETCPEAKLPKFTDLLMKCIWRNVKMLPERSNELNYDAVILEVHEFMLALPSTWWQNRPSDTPMRTIKTILHNMAKVKGNAILQHLNQIPTHSELHTYLIRILKNFQKDGSASGIGASPQRAKEIASKRISHQTHDTVSQIFKLISDRDTKQQGLQKLYDFKQQNPDIDLSTFLQGSSAPFHKYIEEGLAEIERNQNAGSTQDNRTAATRSYLTDVNYQNNGPDPDFWMDRLQYHMTGGAAKLASARSADDGSHMLDNKVVDENLCLNGMNAQKASLIKREKRDMSPNRLQHLQAKLAQIKKENHAQ

Target ORF information:

RefSeq Version	NM_001260202.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mini spindles (msps), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260202.1

ORF Insert Sequence:

ATGGCCGAGG ACACAGAGTA CAAGAAGTTG CCGGTGGAGG AGCGGTGTGT CCATAAACTA 
TGGAAAGCCC GCGTCGATGG TTACGAGGAG GCCGCAAAGA TCTTTCGCGA GCTGGATGAC 
GAGAAGTCGC CGGAATGGTC TAAATTTGCA GGGCTCATAA AGAAGATGGT AGTGGACTCT 
AATGCTCTGG CCCAGGAAAA GGGTCTAGAA GCGGCCCTCA TTTTTGTGGA GAACAGCGGG 
CTGGCGGGAC GCACAGTAGG CGACGTGATG ACAGGCATCG TTCAGAAGTG CATTGCGGCG 
CCAAAGACCA AAACCAAGGA GCTCTCCGTC CAAGTGGCTC TAATGTATGT GGAGATAGAG 
AAGCAGGAGG CCGTCGTCGA AGAGCTGGTT AAAGGTATGG AGGCCAAGAA CCCTAAGATT 
GTGTCTGCCT GCGTGGCAGC CACCACACTG GCCCTCCGCG AGTTCGGACA CAAGGTGATC 
GGCGTCAAGC CGTTAATTAA AAAGCTTGCT CCGCTAATGT CTGACCGAGA CAAGACGGTC 
CGAGATGAGG GCAAGCAGCT GGCTGTTGAG ATTTACCGTT GGATCGGAGC TGCAATGAAG 
GCACAGATCT CAACGCTACC ACAGGTAACG CTAAAGGAGC TGGAGGATGA ATTCGACAAA 
CTCAAAGGTG AACGCGTTGA ACCCTCCAGA TATCTAAAGT CCCAGCAGGA AAAGCAAGCG 
AAGATCGCCG ATGCTGCAGC GACCGAGGAT GCATATAATG AGGATGATGG CGAGGCTGGC 
GTTGAAGAAA TCGATCCTAT GGATCTTCTA GACCCCGTTG ACATTCTCTC CAAAATGCCC 
AAGGACTTCT ACGATAAGCT GGAGGAAAAG AAATGGACTC TGCGCAAAGA ATCGTTGGAA 
GTCCTGGAGA AACTGCTCAC TGACCACCCC AAGCTAGAGA ATGGCGAGTA TGGAGCGCTG 
GTAAGCGCTC TTAAAAAGGT TATAACCAAG GACTCCAACG TAGTCCTCGT TGCAATGGCC 
GGAAAATGTC TAGCCTTGCT AGCCAAAGGA TTGGCAAAGC GATTCTCAAA TTACGCATCC 
GCTTGTGTCC CATCCCTGTT GGAAAAATTT AAAGAGAAGA AACCTAATGT TGTGACAGCG 
CTGCGTGAAG CTATTGATGC CATATACGCC TCCACTTCTC TAGAGGCCCA GCAAGAGTCC 
ATTGTAGAGT CCCTGTCCAA CAAAAATCCA AGTGTTAAGT CCGAAACGGC CTTGTTTATT 
GCCCGTGCCC TAACCCGCAC CCAACCAACC GCGCTAAACA AAAAGCTGCT TAAACTTCTG 
ACCACCAGTC TAGTGAAAAC GCTTAACGAA CCAGATCCAA CCGTACGCGA CAGCAGCGCC 
GAAGCTCTTG GTACTTTAAT AAAGCTAATG GGAGACAAAG CGGTGACTCC TTTGCTCGCC 
GACGTCGATC CACTCAAAAT GGCCAAGATA AAGGAGTGCC AAGAAAAGGC GGAGATTAAA 
ATAAAGGTTG CAGGTCCAAA GAAGGAGACA CGACCTGCAT CTGCACCGAC TGCGAAAGCT 
GCTGCTCCGG CTAAGACAGT GGCTGGTAGT GTGGACCCTA AGCCAGTAAC CCGACCGGCC 
ACTACAGGGG CACGTAAGGT CTTGAAAAAA CCGGCGACGG TTAGTGGAGG AGGAGCTACA 
TCTGCCCCAA CTGCAGCTCT TAAAGCGGGT GGAAAACCCC TAGCCACGGA ACGCGAAATA 
ACCCCCGAGG AATTGCAAGA AAAATCCGAA GAAATTCTGC CAGCCGAAAT TCTTAATGGG 
CTGGTGGATT CCAACTGGAA GAACCGCCTT GCTGCTGTGG AACAGCTTCT GGGCGAAATA 
AGCGGTTTTG ATGCAAAACA GGCTGGCATA TCACAGATCC TGATCCGAAC AATCAGTGGA 
CGAAAGCCAG GCCTCAAAGA AATGAATTTC CAGGTACTCA AGTTTAAGCT AGACATAATC 
CGCAGTGTGG CTGAAAACTA TCCACTGACC ACGACAACCG TTGATCTGGT GATTAACGAA 
ATAATCGAGA AGCTAGCAGA CGCCAAGAAT GGTGCAGCAG CTGCAGATGT TTTGTCTGCC 
TTTGCCGAAG CCACTAAACT GGAGTATGTT GTGGGCAAAG TATTGAGCTT TGCTTTTGAA 
CAAAAATCGC CTAAAGTGCA ATCAGAGGCC TTTAACTGGG TTAACAGGTC AATCATAGAA 
TTCGGCTTCC AATTGCAGCC AAAGACACTT ATTGAAGATG TACGAAAAGG CGTGCAGAGC 
ACGAATCCAA CGGTTCGTGC TTCCGCCATT CAAATGGTAG GCACCATGTC TATGTATATG 
GGCAAAGCTC TAATGATGTT CTTCGACAGC GAGAAGCCTG CACTAAAGTC CCAGATCCAG 
GTAGAGTTTG ACAAGAATGT GGGCGAAAAA CCACCCAAGC CTGTTAGAGG AGTTCAACGT 
AGCAGCGGTG GAACCGCTGG TAATTCCCCA GACAATGAGG ACGATGATGG TGGGGCAGCC 
GGAGAAGAGG AGCCCATTAA TATGGCTGAT CTTCTACCAC GCGTTGATAT TGCTCCACAG 
ATTACGGAGG CATTGCTGAA GGAGATGTCC GACAAGGACT GGAAAACTCG CAACGAGGGT 
CTGACTAAGC TGCAGGCTAT TATTTCAGAG GCGCGGCTAA TCAAACCCAG CATTGGCGAC 
TTGGCTCCTG CTCTGGCCCA CCGTCTGGTA GATTCCAATG CTAAGATTGC CCAAACGACA 
CTTGCCATTT GCGAGCAGCT TGCCACAGCC ATGGGGGCTG GTTGTCGAAA TCACGTGCGG 
AACTTGTTCC CTGGCTTTTT ACACGCTCTG GGTGATAACA AGAGTTTTGT AAGAGCCGCT 
GCCCTCAATT GCATCAATAG TTTTGGCGAA AAGGGCGGCT ATAAGGAATT TTTTGAGAGC 
GAAATGATAG CCGATGCCCT GAAAGGAGGA TCCCCAGCTC TAAAGACAGA ATTGTGGGCT 
TGGCTGGCAG ATAAGCTGCC TGGTCTTCCC CCCAAGTCAG TATCGAAGGA GGATATCCAT 
TCAATGGTGC CGCACTTATA TGCTCACATC TGCGATCGAA ACGCCGATGT CCGGAAAAAT 
GCCAACGAGG CGGTGTTGGG CATTATGATT CACCTTGGAT TTGATGCAAT GAACCGCGCT 
CTGGACAAAC AGAAGCCCGC GTCCAAAAAG GATATACTTG CAGCACTCGA AAAAGCGCGT 
CCTAATCTTC CGGTAAAACC GCTGCCCAAA GGCAAGCATC AGGCTCCAAT ACCTGAGGAG 
CCGAAGCTAA AAACAGTGCG CGGCGGTGGA GCAGGTGGTG CGCCTGGAAT CCAGAAGAGT 
GCTACAGCCC GGGTTGCCGG AGGACAAGAC AAGCAGGTAC CAGCGCGCAA AAAGGACGAA 
GATATCGACA CATCGCCGCT ACTGTGCGCC AACAGTGCTA AAAACCAGCG GCTGCTAGAC 
GAGCAAAAAA TGAAGGTACT AAAGTGGACT TTTGTAACCC CAAGAGAGGA ATTCACCGAA 
CTGTTACGCG ACCAAATGAT GACCGCAAAC GTAAATAAAG CACTGATAGC CAACATGTTC 
CACGACGATT TTCGCTACCA CTTGAAAGTC ATCGAGCAGT TGAGCGAAGA TCTGGCTGGT 
AACAGTAAGG CGCTGGTGTG TAATCTGGAC CTAATACTTA AATGGCTGAC GCTGCGATTT 
TACGATACGA ACCCTTCTGT GTTGATTAAG GGTCTCGAAT ATCTAGTACA GGTTTTCCAG 
GTACTCATTG ACGAGGAATA CATTCTGGCC GAGAACGAGG GAAGCAGTTT TGTCCCACAT 
CTGCTTTTAA AGATCGGAGA TCCAAAGGAT GCTGTTCGAA ATGGCGTACG CCGCGTTCTG 
CGGCAAGTAA TATTGGTCTT CCCTTTCGTC AAGGTCTTTG GCTATGTGAT GGAGGGTCTA 
AAATCGAAGA ATGCCCGTCA GCGTACTGAG TGCCTTGACG AGTTAACCTT CCTAATTGAA 
AGCTATGGCA TGAACATCTG TCCCCAATCG GCGGTGCGCG AGATTGCTCG CCAGATTTCC 
GACCGGGATA ATTCCGTACG CAACGCAGCA CTCAACTGCA TCGTCCAGGT CTTCTTTTTG 
TCCGGGGAAA AGACTTACAA GATGATTGGC CATCTTAACG AAAAGGACCT CTCAATGCTA 
GATGAGCGCA TCAAGCGAGC CAAAAAGACT AAGAAGCCCA CGCCACCGCC ATCTGTTGAT 
GTGCCGGCAC CTCAACGACA TGACAGCATC GAAATCGAAG ATGCTGAAGT GGGCAACGGT 
TGCGACGAGC TTCCACCACC AGATGAGGAC GGAACTTTCG ACCAGGCACC TTCGTCACAG 
CTGCTTCTAC TCCAGCAACA GCTTCAACAG TTACAACAGC AGGCCCAGCA ACAGAAGCCC 
AGCGGACCAT TCGGCTTGGA CTCGCAGGTA ATCAGCGAGA TCGAAAAAGA TTGGGTCCGA 
GTGGACCAAA TGGAGCAGAA GCCGCTACTG AACGTAGATA TCTCATCGCT GGACGAGCCT 
ATTAAGGTGA GGCCCACGCG GGCCGGCATT CACTACCCGC AGGAGAAGTT CGATCGGCTG 
ATTTCTCGAC AACACTATAT GCAGCAGACT TTAACGACGT CCCCGTCATC CACGGCTGGA 
ATGACCAGTG GAGTCTCCCC GTATCGCAGT CCTATGCGCT TGCAGCACCA GCAGCCTCAA 
CAGCAGCTGG AGAACAATAT ACCAAATTTG GCGGATGTTT TGCCAAAGCA CGATCCGCAG 
CTGGTAAAAG TTATAAAAGG TGTAAGCAGC ACGGACACTC TAAAGGCGCG CGCGGCTATT 
AACGAGCTTG CCGCCATCAT CGAGGCTCCA GAGAAGCAGG CTGTGCTGCG CGACTATGAA 
GAAATATTTA TTCAGAATGT GCTTGCACAG TTTAAGAATC TCTCACAAAT ACCATCGGCT 
CAATCCGTAG TTGTTTACCA GCCGCTATTA TCCATTCTGT ATACATTTTT CCATGCCAAC 
ATTCTGGGCA AAACGCTGAG CGTGGCCTGC ATCAAAAATC TAATGTCGGC GCTGTTGAAC 
CTTATGGCTG ATCCGAAGTT GGCCGTGGGT GATGATAGTC AGTATAACAA GGTTATTAAT 
GGCATATGTC TAAAAGTGTT GGACAAGGTA GATTTTACGA ATTTAAACTG TGCTTTGATA 
CGCCTATTAC GGGAGACTTG TCCGGAGGCA AAGCTGCCGA AGTTTACAGA CCTGCTGATG 
AAGTGCATAT GGCGCAACGT TAAGATGCTA CCCGAACGAA GCAACGAGCT GAACTATGAC 
GCCGTAATTC TCGAGGTGCA CGAGTTCATG CTCGCGCTGC CTAGCACTTG GTGGCAAAAC 
CGACCGTCGG ACACTCCAAT GCGCACGATT AAGACCATTC TGCATAACAT GGCAAAGGTG 
AAGGGGAATG CTATTCTGCA GCATCTCAAC CAGATTCCAA CACACTCGGA GCTACATACT 
TATTTGATAC GCATTCTCAA GAATTTCCAA AAGGATGGCT CTGCTTCAGG AATTGGTGCC 
TCCCCCCAGA GGGCCAAGGA GATTGCCTCC AAGCGAATTT CGCACCAAAC ACATGATACA 
GTATCACAGA TCTTTAAGCT GATCTCAGAT AGAGATACCA AACAGCAAGG TCTGCAGAAA 
CTTTACGACT TTAAGCAACA AAATCCTGAT ATCGATCTAA GTACCTTTCT GCAAGGCTCC 
AGTGCCCCAT TTCACAAGTA CATCGAGGAG GGTTTAGCCG AGATTGAGAG GAATCAGAAC 
GCTGGATCCA CACAGGATAA TCGGACGGCA GCTACTCGTT CTTATCTCAC AGATGTCAAC 
TACCAGAACA ACGGTCCCGA TCCCGATTTC TGGATGGACC GCCTGCAGTA TCATATGACC 
GGAGGGGCCG CCAAGCTAGC ATCTGCACGT TCAGCAGACG ATGGATCCCA TATGCTGGAC 
AATAAGGTGG TTGACGAGAA CCTCTGCCTG AACGGAATGA ACGCGCAAAA GGCGTCGCTC 
ATTAAGCGAG AAAAGCGGGA CATGTCACCA AATCGCTTGC AGCATCTCCA GGCAAAGCTG 
GCTCAAATCA AGAAAGAGAA TCATGCCCAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

msps ( NM_001170156.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163627.1 Drosophila melanogaster mini spindles (msps), transcript variant B, mRNA.

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CloneID	OFa08421
Clone ID Related Accession (Same CDS sequence)	NM_001260202.1 , NM_001170156.2
Accession Version	NM_001170156.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mini spindles, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170156.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGAGG ACACAGAGTA CAAGAAGTTG CCGGTGGAGG AGCGGTGTGT CCATAAACTA 
TGGAAAGCCC GCGTCGATGG TTACGAGGAG GCCGCAAAGA TCTTTCGCGA GCTGGATGAC 
GAGAAGTCGC CGGAATGGTC TAAATTTGCA GGGCTCATAA AGAAGATGGT AGTGGACTCT 
AATGCTCTGG CCCAGGAAAA GGGTCTAGAA GCGGCCCTCA TTTTTGTGGA GAACAGCGGG 
CTGGCGGGAC GCACAGTAGG CGACGTGATG ACAGGCATCG TTCAGAAGTG CATTGCGGCG 
CCAAAGACCA AAACCAAGGA GCTCTCCGTC CAAGTGGCTC TAATGTATGT GGAGATAGAG 
AAGCAGGAGG CCGTCGTCGA AGAGCTGGTT AAAGGTATGG AGGCCAAGAA CCCTAAGATT 
GTGTCTGCCT GCGTGGCAGC CACCACACTG GCCCTCCGCG AGTTCGGACA CAAGGTGATC 
GGCGTCAAGC CGTTAATTAA AAAGCTTGCT CCGCTAATGT CTGACCGAGA CAAGACGGTC 
CGAGATGAGG GCAAGCAGCT GGCTGTTGAG ATTTACCGTT GGATCGGAGC TGCAATGAAG 
GCACAGATCT CAACGCTACC ACAGGTAACG CTAAAGGAGC TGGAGGATGA ATTCGACAAA 
CTCAAAGGTG AACGCGTTGA ACCCTCCAGA TATCTAAAGT CCCAGCAGGA AAAGCAAGCG 
AAGATCGCCG ATGCTGCAGC GACCGAGGAT GCATATAATG AGGATGATGG CGAGGCTGGC 
GTTGAAGAAA TCGATCCTAT GGATCTTCTA GACCCCGTTG ACATTCTCTC CAAAATGCCC 
AAGGACTTCT ACGATAAGCT GGAGGAAAAG AAATGGACTC TGCGCAAAGA ATCGTTGGAA 
GTCCTGGAGA AACTGCTCAC TGACCACCCC AAGCTAGAGA ATGGCGAGTA TGGAGCGCTG 
GTAAGCGCTC TTAAAAAGGT TATAACCAAG GACTCCAACG TAGTCCTCGT TGCAATGGCC 
GGAAAATGTC TAGCCTTGCT AGCCAAAGGA TTGGCAAAGC GATTCTCAAA TTACGCATCC 
GCTTGTGTCC CATCCCTGTT GGAAAAATTT AAAGAGAAGA AACCTAATGT TGTGACAGCG 
CTGCGTGAAG CTATTGATGC CATATACGCC TCCACTTCTC TAGAGGCCCA GCAAGAGTCC 
ATTGTAGAGT CCCTGTCCAA CAAAAATCCA AGTGTTAAGT CCGAAACGGC CTTGTTTATT 
GCCCGTGCCC TAACCCGCAC CCAACCAACC GCGCTAAACA AAAAGCTGCT TAAACTTCTG 
ACCACCAGTC TAGTGAAAAC GCTTAACGAA CCAGATCCAA CCGTACGCGA CAGCAGCGCC 
GAAGCTCTTG GTACTTTAAT AAAGCTAATG GGAGACAAAG CGGTGACTCC TTTGCTCGCC 
GACGTCGATC CACTCAAAAT GGCCAAGATA AAGGAGTGCC AAGAAAAGGC GGAGATTAAA 
ATAAAGGTTG CAGGTCCAAA GAAGGAGACA CGACCTGCAT CTGCACCGAC TGCGAAAGCT 
GCTGCTCCGG CTAAGACAGT GGCTGGTAGT GTGGACCCTA AGCCAGTAAC CCGACCGGCC 
ACTACAGGGG CACGTAAGGT CTTGAAAAAA CCGGCGACGG TTAGTGGAGG AGGAGCTACA 
TCTGCCCCAA CTGCAGCTCT TAAAGCGGGT GGAAAACCCC TAGCCACGGA ACGCGAAATA 
ACCCCCGAGG AATTGCAAGA AAAATCCGAA GAAATTCTGC CAGCCGAAAT TCTTAATGGG 
CTGGTGGATT CCAACTGGAA GAACCGCCTT GCTGCTGTGG AACAGCTTCT GGGCGAAATA 
AGCGGTTTTG ATGCAAAACA GGCTGGCATA TCACAGATCC TGATCCGAAC AATCAGTGGA 
CGAAAGCCAG GCCTCAAAGA AATGAATTTC CAGGTACTCA AGTTTAAGCT AGACATAATC 
CGCAGTGTGG CTGAAAACTA TCCACTGACC ACGACAACCG TTGATCTGGT GATTAACGAA 
ATAATCGAGA AGCTAGCAGA CGCCAAGAAT GGTGCAGCAG CTGCAGATGT TTTGTCTGCC 
TTTGCCGAAG CCACTAAACT GGAGTATGTT GTGGGCAAAG TATTGAGCTT TGCTTTTGAA 
CAAAAATCGC CTAAAGTGCA ATCAGAGGCC TTTAACTGGG TTAACAGGTC AATCATAGAA 
TTCGGCTTCC AATTGCAGCC AAAGACACTT ATTGAAGATG TACGAAAAGG CGTGCAGAGC 
ACGAATCCAA CGGTTCGTGC TTCCGCCATT CAAATGGTAG GCACCATGTC TATGTATATG 
GGCAAAGCTC TAATGATGTT CTTCGACAGC GAGAAGCCTG CACTAAAGTC CCAGATCCAG 
GTAGAGTTTG ACAAGAATGT GGGCGAAAAA CCACCCAAGC CTGTTAGAGG AGTTCAACGT 
AGCAGCGGTG GAACCGCTGG TAATTCCCCA GACAATGAGG ACGATGATGG TGGGGCAGCC 
GGAGAAGAGG AGCCCATTAA TATGGCTGAT CTTCTACCAC GCGTTGATAT TGCTCCACAG 
ATTACGGAGG CATTGCTGAA GGAGATGTCC GACAAGGACT GGAAAACTCG CAACGAGGGT 
CTGACTAAGC TGCAGGCTAT TATTTCAGAG GCGCGGCTAA TCAAACCCAG CATTGGCGAC 
TTGGCTCCTG CTCTGGCCCA CCGTCTGGTA GATTCCAATG CTAAGATTGC CCAAACGACA 
CTTGCCATTT GCGAGCAGCT TGCCACAGCC ATGGGGGCTG GTTGTCGAAA TCACGTGCGG 
AACTTGTTCC CTGGCTTTTT ACACGCTCTG GGTGATAACA AGAGTTTTGT AAGAGCCGCT 
GCCCTCAATT GCATCAATAG TTTTGGCGAA AAGGGCGGCT ATAAGGAATT TTTTGAGAGC 
GAAATGATAG CCGATGCCCT GAAAGGAGGA TCCCCAGCTC TAAAGACAGA ATTGTGGGCT 
TGGCTGGCAG ATAAGCTGCC TGGTCTTCCC CCCAAGTCAG TATCGAAGGA GGATATCCAT 
TCAATGGTGC CGCACTTATA TGCTCACATC TGCGATCGAA ACGCCGATGT CCGGAAAAAT 
GCCAACGAGG CGGTGTTGGG CATTATGATT CACCTTGGAT TTGATGCAAT GAACCGCGCT 
CTGGACAAAC AGAAGCCCGC GTCCAAAAAG GATATACTTG CAGCACTCGA AAAAGCGCGT 
CCTAATCTTC CGGTAAAACC GCTGCCCAAA GGCAAGCATC AGGCTCCAAT ACCTGAGGAG 
CCGAAGCTAA AAACAGTGCG CGGCGGTGGA GCAGGTGGTG CGCCTGGAAT CCAGAAGAGT 
GCTACAGCCC GGGTTGCCGG AGGACAAGAC AAGCAGGTAC CAGCGCGCAA AAAGGACGAA 
GATATCGACA CATCGCCGCT ACTGTGCGCC AACAGTGCTA AAAACCAGCG GCTGCTAGAC 
GAGCAAAAAA TGAAGGTACT AAAGTGGACT TTTGTAACCC CAAGAGAGGA ATTCACCGAA 
CTGTTACGCG ACCAAATGAT GACCGCAAAC GTAAATAAAG CACTGATAGC CAACATGTTC 
CACGACGATT TTCGCTACCA CTTGAAAGTC ATCGAGCAGT TGAGCGAAGA TCTGGCTGGT 
AACAGTAAGG CGCTGGTGTG TAATCTGGAC CTAATACTTA AATGGCTGAC GCTGCGATTT 
TACGATACGA ACCCTTCTGT GTTGATTAAG GGTCTCGAAT ATCTAGTACA GGTTTTCCAG 
GTACTCATTG ACGAGGAATA CATTCTGGCC GAGAACGAGG GAAGCAGTTT TGTCCCACAT 
CTGCTTTTAA AGATCGGAGA TCCAAAGGAT GCTGTTCGAA ATGGCGTACG CCGCGTTCTG 
CGGCAAGTAA TATTGGTCTT CCCTTTCGTC AAGGTCTTTG GCTATGTGAT GGAGGGTCTA 
AAATCGAAGA ATGCCCGTCA GCGTACTGAG TGCCTTGACG AGTTAACCTT CCTAATTGAA 
AGCTATGGCA TGAACATCTG TCCCCAATCG GCGGTGCGCG AGATTGCTCG CCAGATTTCC 
GACCGGGATA ATTCCGTACG CAACGCAGCA CTCAACTGCA TCGTCCAGGT CTTCTTTTTG 
TCCGGGGAAA AGACTTACAA GATGATTGGC CATCTTAACG AAAAGGACCT CTCAATGCTA 
GATGAGCGCA TCAAGCGAGC CAAAAAGACT AAGAAGCCCA CGCCACCGCC ATCTGTTGAT 
GTGCCGGCAC CTCAACGACA TGACAGCATC GAAATCGAAG ATGCTGAAGT GGGCAACGGT 
TGCGACGAGC TTCCACCACC AGATGAGGAC GGAACTTTCG ACCAGGCACC TTCGTCACAG 
CTGCTTCTAC TCCAGCAACA GCTTCAACAG TTACAACAGC AGGCCCAGCA ACAGAAGCCC 
AGCGGACCAT TCGGCTTGGA CTCGCAGGTA ATCAGCGAGA TCGAAAAAGA TTGGGTCCGA 
GTGGACCAAA TGGAGCAGAA GCCGCTACTG AACGTAGATA TCTCATCGCT GGACGAGCCT 
ATTAAGGTGA GGCCCACGCG GGCCGGCATT CACTACCCGC AGGAGAAGTT CGATCGGCTG 
ATTTCTCGAC AACACTATAT GCAGCAGACT TTAACGACGT CCCCGTCATC CACGGCTGGA 
ATGACCAGTG GAGTCTCCCC GTATCGCAGT CCTATGCGCT TGCAGCACCA GCAGCCTCAA 
CAGCAGCTGG AGAACAATAT ACCAAATTTG GCGGATGTTT TGCCAAAGCA CGATCCGCAG 
CTGGTAAAAG TTATAAAAGG TGTAAGCAGC ACGGACACTC TAAAGGCGCG CGCGGCTATT 
AACGAGCTTG CCGCCATCAT CGAGGCTCCA GAGAAGCAGG CTGTGCTGCG CGACTATGAA 
GAAATATTTA TTCAGAATGT GCTTGCACAG TTTAAGAATC TCTCACAAAT ACCATCGGCT 
CAATCCGTAG TTGTTTACCA GCCGCTATTA TCCATTCTGT ATACATTTTT CCATGCCAAC 
ATTCTGGGCA AAACGCTGAG CGTGGCCTGC ATCAAAAATC TAATGTCGGC GCTGTTGAAC 
CTTATGGCTG ATCCGAAGTT GGCCGTGGGT GATGATAGTC AGTATAACAA GGTTATTAAT 
GGCATATGTC TAAAAGTGTT GGACAAGGTA GATTTTACGA ATTTAAACTG TGCTTTGATA 
CGCCTATTAC GGGAGACTTG TCCGGAGGCA AAGCTGCCGA AGTTTACAGA CCTGCTGATG 
AAGTGCATAT GGCGCAACGT TAAGATGCTA CCCGAACGAA GCAACGAGCT GAACTATGAC 
GCCGTAATTC TCGAGGTGCA CGAGTTCATG CTCGCGCTGC CTAGCACTTG GTGGCAAAAC 
CGACCGTCGG ACACTCCAAT GCGCACGATT AAGACCATTC TGCATAACAT GGCAAAGGTG 
AAGGGGAATG CTATTCTGCA GCATCTCAAC CAGATTCCAA CACACTCGGA GCTACATACT 
TATTTGATAC GCATTCTCAA GAATTTCCAA AAGGATGGCT CTGCTTCAGG AATTGGTGCC 
TCCCCCCAGA GGGCCAAGGA GATTGCCTCC AAGCGAATTT CGCACCAAAC ACATGATACA 
GTATCACAGA TCTTTAAGCT GATCTCAGAT AGAGATACCA AACAGCAAGG TCTGCAGAAA 
CTTTACGACT TTAAGCAACA AAATCCTGAT ATCGATCTAA GTACCTTTCT GCAAGGCTCC 
AGTGCCCCAT TTCACAAGTA CATCGAGGAG GGTTTAGCCG AGATTGAGAG GAATCAGAAC 
GCTGGATCCA CACAGGATAA TCGGACGGCA GCTACTCGTT CTTATCTCAC AGATGTCAAC 
TACCAGAACA ACGGTCCCGA TCCCGATTTC TGGATGGACC GCCTGCAGTA TCATATGACC 
GGAGGGGCCG CCAAGCTAGC ATCTGCACGT TCAGCAGACG ATGGATCCCA TATGCTGGAC 
AATAAGGTGG TTGACGAGAA CCTCTGCCTG AACGGAATGA ACGCGCAAAA GGCGTCGCTC 
ATTAAGCGAG AAAAGCGGGA CATGTCACCA AATCGCTTGC AGCATCTCCA GGCAAAGCTG 
GCTCAAATCA AGAAAGAGAA TCATGCCCAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163627.1
CDS	447..6599
Translation	MAEDTEYKKLPVEERCVHKLWKARVDGYEEAAKIFRELDDEKSPEWSKFAGLIKKMVVDSNALAQEKGLEAALIFVENSGLAGRTVGDVMTGIVQKCIAAPKTKTKELSVQVALMYVEIEKQEAVVEELVKGMEAKNPKIVSACVAATTLALREFGHKVIGVKPLIKKLAPLMSDRDKTVRDEGKQLAVEIYRWIGAAMKAQISTLPQVTLKELEDEFDKLKGERVEPSRYLKSQQEKQAKIADAAATEDAYNEDDGEAGVEEIDPMDLLDPVDILSKMPKDFYDKLEEKKWTLRKESLEVLEKLLTDHPKLENGEYGALVSALKKVITKDSNVVLVAMAGKCLALLAKGLAKRFSNYASACVPSLLEKFKEKKPNVVTALREAIDAIYASTSLEAQQESIVESLSNKNPSVKSETALFIARALTRTQPTALNKKLLKLLTTSLVKTLNEPDPTVRDSSAEALGTLIKLMGDKAVTPLLADVDPLKMAKIKECQEKAEIKIKVAGPKKETRPASAPTAKAAAPAKTVAGSVDPKPVTRPATTGARKVLKKPATVSGGGATSAPTAALKAGGKPLATEREITPEELQEKSEEILPAEILNGLVDSNWKNRLAAVEQLLGEISGFDAKQAGISQILIRTISGRKPGLKEMNFQVLKFKLDIIRSVAENYPLTTTTVDLVINEIIEKLADAKNGAAAADVLSAFAEATKLEYVVGKVLSFAFEQKSPKVQSEAFNWVNRSIIEFGFQLQPKTLIEDVRKGVQSTNPTVRASAIQMVGTMSMYMGKALMMFFDSEKPALKSQIQVEFDKNVGEKPPKPVRGVQRSSGGTAGNSPDNEDDDGGAAGEEEPINMADLLPRVDIAPQITEALLKEMSDKDWKTRNEGLTKLQAIISEARLIKPSIGDLAPALAHRLVDSNAKIAQTTLAICEQLATAMGAGCRNHVRNLFPGFLHALGDNKSFVRAAALNCINSFGEKGGYKEFFESEMIADALKGGSPALKTELWAWLADKLPGLPPKSVSKEDIHSMVPHLYAHICDRNADVRKNANEAVLGIMIHLGFDAMNRALDKQKPASKKDILAALEKARPNLPVKPLPKGKHQAPIPEEPKLKTVRGGGAGGAPGIQKSATARVAGGQDKQVPARKKDEDIDTSPLLCANSAKNQRLLDEQKMKVLKWTFVTPREEFTELLRDQMMTANVNKALIANMFHDDFRYHLKVIEQLSEDLAGNSKALVCNLDLILKWLTLRFYDTNPSVLIKGLEYLVQVFQVLIDEEYILAENEGSSFVPHLLLKIGDPKDAVRNGVRRVLRQVILVFPFVKVFGYVMEGLKSKNARQRTECLDELTFLIESYGMNICPQSAVREIARQISDRDNSVRNAALNCIVQVFFLSGEKTYKMIGHLNEKDLSMLDERIKRAKKTKKPTPPPSVDVPAPQRHDSIEIEDAEVGNGCDELPPPDEDGTFDQAPSSQLLLLQQQLQQLQQQAQQQKPSGPFGLDSQVISEIEKDWVRVDQMEQKPLLNVDISSLDEPIKVRPTRAGIHYPQEKFDRLISRQHYMQQTLTTSPSSTAGMTSGVSPYRSPMRLQHQQPQQQLENNIPNLADVLPKHDPQLVKVIKGVSSTDTLKARAAINELAAIIEAPEKQAVLRDYEEIFIQNVLAQFKNLSQIPSAQSVVVYQPLLSILYTFFHANILGKTLSVACIKNLMSALLNLMADPKLAVGDDSQYNKVINGICLKVLDKVDFTNLNCALIRLLRETCPEAKLPKFTDLLMKCIWRNVKMLPERSNELNYDAVILEVHEFMLALPSTWWQNRPSDTPMRTIKTILHNMAKVKGNAILQHLNQIPTHSELHTYLIRILKNFQKDGSASGIGASPQRAKEIASKRISHQTHDTVSQIFKLISDRDTKQQGLQKLYDFKQQNPDIDLSTFLQGSSAPFHKYIEEGLAEIERNQNAGSTQDNRTAATRSYLTDVNYQNNGPDPDFWMDRLQYHMTGGAAKLASARSADDGSHMLDNKVVDENLCLNGMNAQKASLIKREKRDMSPNRLQHLQAKLAQIKKENHAQ

Target ORF information:

RefSeq Version	NM_001170156.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mini spindles (msps), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170156.2

ORF Insert Sequence:

ATGGCCGAGG ACACAGAGTA CAAGAAGTTG CCGGTGGAGG AGCGGTGTGT CCATAAACTA 
TGGAAAGCCC GCGTCGATGG TTACGAGGAG GCCGCAAAGA TCTTTCGCGA GCTGGATGAC 
GAGAAGTCGC CGGAATGGTC TAAATTTGCA GGGCTCATAA AGAAGATGGT AGTGGACTCT 
AATGCTCTGG CCCAGGAAAA GGGTCTAGAA GCGGCCCTCA TTTTTGTGGA GAACAGCGGG 
CTGGCGGGAC GCACAGTAGG CGACGTGATG ACAGGCATCG TTCAGAAGTG CATTGCGGCG 
CCAAAGACCA AAACCAAGGA GCTCTCCGTC CAAGTGGCTC TAATGTATGT GGAGATAGAG 
AAGCAGGAGG CCGTCGTCGA AGAGCTGGTT AAAGGTATGG AGGCCAAGAA CCCTAAGATT 
GTGTCTGCCT GCGTGGCAGC CACCACACTG GCCCTCCGCG AGTTCGGACA CAAGGTGATC 
GGCGTCAAGC CGTTAATTAA AAAGCTTGCT CCGCTAATGT CTGACCGAGA CAAGACGGTC 
CGAGATGAGG GCAAGCAGCT GGCTGTTGAG ATTTACCGTT GGATCGGAGC TGCAATGAAG 
GCACAGATCT CAACGCTACC ACAGGTAACG CTAAAGGAGC TGGAGGATGA ATTCGACAAA 
CTCAAAGGTG AACGCGTTGA ACCCTCCAGA TATCTAAAGT CCCAGCAGGA AAAGCAAGCG 
AAGATCGCCG ATGCTGCAGC GACCGAGGAT GCATATAATG AGGATGATGG CGAGGCTGGC 
GTTGAAGAAA TCGATCCTAT GGATCTTCTA GACCCCGTTG ACATTCTCTC CAAAATGCCC 
AAGGACTTCT ACGATAAGCT GGAGGAAAAG AAATGGACTC TGCGCAAAGA ATCGTTGGAA 
GTCCTGGAGA AACTGCTCAC TGACCACCCC AAGCTAGAGA ATGGCGAGTA TGGAGCGCTG 
GTAAGCGCTC TTAAAAAGGT TATAACCAAG GACTCCAACG TAGTCCTCGT TGCAATGGCC 
GGAAAATGTC TAGCCTTGCT AGCCAAAGGA TTGGCAAAGC GATTCTCAAA TTACGCATCC 
GCTTGTGTCC CATCCCTGTT GGAAAAATTT AAAGAGAAGA AACCTAATGT TGTGACAGCG 
CTGCGTGAAG CTATTGATGC CATATACGCC TCCACTTCTC TAGAGGCCCA GCAAGAGTCC 
ATTGTAGAGT CCCTGTCCAA CAAAAATCCA AGTGTTAAGT CCGAAACGGC CTTGTTTATT 
GCCCGTGCCC TAACCCGCAC CCAACCAACC GCGCTAAACA AAAAGCTGCT TAAACTTCTG 
ACCACCAGTC TAGTGAAAAC GCTTAACGAA CCAGATCCAA CCGTACGCGA CAGCAGCGCC 
GAAGCTCTTG GTACTTTAAT AAAGCTAATG GGAGACAAAG CGGTGACTCC TTTGCTCGCC 
GACGTCGATC CACTCAAAAT GGCCAAGATA AAGGAGTGCC AAGAAAAGGC GGAGATTAAA 
ATAAAGGTTG CAGGTCCAAA GAAGGAGACA CGACCTGCAT CTGCACCGAC TGCGAAAGCT 
GCTGCTCCGG CTAAGACAGT GGCTGGTAGT GTGGACCCTA AGCCAGTAAC CCGACCGGCC 
ACTACAGGGG CACGTAAGGT CTTGAAAAAA CCGGCGACGG TTAGTGGAGG AGGAGCTACA 
TCTGCCCCAA CTGCAGCTCT TAAAGCGGGT GGAAAACCCC TAGCCACGGA ACGCGAAATA 
ACCCCCGAGG AATTGCAAGA AAAATCCGAA GAAATTCTGC CAGCCGAAAT TCTTAATGGG 
CTGGTGGATT CCAACTGGAA GAACCGCCTT GCTGCTGTGG AACAGCTTCT GGGCGAAATA 
AGCGGTTTTG ATGCAAAACA GGCTGGCATA TCACAGATCC TGATCCGAAC AATCAGTGGA 
CGAAAGCCAG GCCTCAAAGA AATGAATTTC CAGGTACTCA AGTTTAAGCT AGACATAATC 
CGCAGTGTGG CTGAAAACTA TCCACTGACC ACGACAACCG TTGATCTGGT GATTAACGAA 
ATAATCGAGA AGCTAGCAGA CGCCAAGAAT GGTGCAGCAG CTGCAGATGT TTTGTCTGCC 
TTTGCCGAAG CCACTAAACT GGAGTATGTT GTGGGCAAAG TATTGAGCTT TGCTTTTGAA 
CAAAAATCGC CTAAAGTGCA ATCAGAGGCC TTTAACTGGG TTAACAGGTC AATCATAGAA 
TTCGGCTTCC AATTGCAGCC AAAGACACTT ATTGAAGATG TACGAAAAGG CGTGCAGAGC 
ACGAATCCAA CGGTTCGTGC TTCCGCCATT CAAATGGTAG GCACCATGTC TATGTATATG 
GGCAAAGCTC TAATGATGTT CTTCGACAGC GAGAAGCCTG CACTAAAGTC CCAGATCCAG 
GTAGAGTTTG ACAAGAATGT GGGCGAAAAA CCACCCAAGC CTGTTAGAGG AGTTCAACGT 
AGCAGCGGTG GAACCGCTGG TAATTCCCCA GACAATGAGG ACGATGATGG TGGGGCAGCC 
GGAGAAGAGG AGCCCATTAA TATGGCTGAT CTTCTACCAC GCGTTGATAT TGCTCCACAG 
ATTACGGAGG CATTGCTGAA GGAGATGTCC GACAAGGACT GGAAAACTCG CAACGAGGGT 
CTGACTAAGC TGCAGGCTAT TATTTCAGAG GCGCGGCTAA TCAAACCCAG CATTGGCGAC 
TTGGCTCCTG CTCTGGCCCA CCGTCTGGTA GATTCCAATG CTAAGATTGC CCAAACGACA 
CTTGCCATTT GCGAGCAGCT TGCCACAGCC ATGGGGGCTG GTTGTCGAAA TCACGTGCGG 
AACTTGTTCC CTGGCTTTTT ACACGCTCTG GGTGATAACA AGAGTTTTGT AAGAGCCGCT 
GCCCTCAATT GCATCAATAG TTTTGGCGAA AAGGGCGGCT ATAAGGAATT TTTTGAGAGC 
GAAATGATAG CCGATGCCCT GAAAGGAGGA TCCCCAGCTC TAAAGACAGA ATTGTGGGCT 
TGGCTGGCAG ATAAGCTGCC TGGTCTTCCC CCCAAGTCAG TATCGAAGGA GGATATCCAT 
TCAATGGTGC CGCACTTATA TGCTCACATC TGCGATCGAA ACGCCGATGT CCGGAAAAAT 
GCCAACGAGG CGGTGTTGGG CATTATGATT CACCTTGGAT TTGATGCAAT GAACCGCGCT 
CTGGACAAAC AGAAGCCCGC GTCCAAAAAG GATATACTTG CAGCACTCGA AAAAGCGCGT 
CCTAATCTTC CGGTAAAACC GCTGCCCAAA GGCAAGCATC AGGCTCCAAT ACCTGAGGAG 
CCGAAGCTAA AAACAGTGCG CGGCGGTGGA GCAGGTGGTG CGCCTGGAAT CCAGAAGAGT 
GCTACAGCCC GGGTTGCCGG AGGACAAGAC AAGCAGGTAC CAGCGCGCAA AAAGGACGAA 
GATATCGACA CATCGCCGCT ACTGTGCGCC AACAGTGCTA AAAACCAGCG GCTGCTAGAC 
GAGCAAAAAA TGAAGGTACT AAAGTGGACT TTTGTAACCC CAAGAGAGGA ATTCACCGAA 
CTGTTACGCG ACCAAATGAT GACCGCAAAC GTAAATAAAG CACTGATAGC CAACATGTTC 
CACGACGATT TTCGCTACCA CTTGAAAGTC ATCGAGCAGT TGAGCGAAGA TCTGGCTGGT 
AACAGTAAGG CGCTGGTGTG TAATCTGGAC CTAATACTTA AATGGCTGAC GCTGCGATTT 
TACGATACGA ACCCTTCTGT GTTGATTAAG GGTCTCGAAT ATCTAGTACA GGTTTTCCAG 
GTACTCATTG ACGAGGAATA CATTCTGGCC GAGAACGAGG GAAGCAGTTT TGTCCCACAT 
CTGCTTTTAA AGATCGGAGA TCCAAAGGAT GCTGTTCGAA ATGGCGTACG CCGCGTTCTG 
CGGCAAGTAA TATTGGTCTT CCCTTTCGTC AAGGTCTTTG GCTATGTGAT GGAGGGTCTA 
AAATCGAAGA ATGCCCGTCA GCGTACTGAG TGCCTTGACG AGTTAACCTT CCTAATTGAA 
AGCTATGGCA TGAACATCTG TCCCCAATCG GCGGTGCGCG AGATTGCTCG CCAGATTTCC 
GACCGGGATA ATTCCGTACG CAACGCAGCA CTCAACTGCA TCGTCCAGGT CTTCTTTTTG 
TCCGGGGAAA AGACTTACAA GATGATTGGC CATCTTAACG AAAAGGACCT CTCAATGCTA 
GATGAGCGCA TCAAGCGAGC CAAAAAGACT AAGAAGCCCA CGCCACCGCC ATCTGTTGAT 
GTGCCGGCAC CTCAACGACA TGACAGCATC GAAATCGAAG ATGCTGAAGT GGGCAACGGT 
TGCGACGAGC TTCCACCACC AGATGAGGAC GGAACTTTCG ACCAGGCACC TTCGTCACAG 
CTGCTTCTAC TCCAGCAACA GCTTCAACAG TTACAACAGC AGGCCCAGCA ACAGAAGCCC 
AGCGGACCAT TCGGCTTGGA CTCGCAGGTA ATCAGCGAGA TCGAAAAAGA TTGGGTCCGA 
GTGGACCAAA TGGAGCAGAA GCCGCTACTG AACGTAGATA TCTCATCGCT GGACGAGCCT 
ATTAAGGTGA GGCCCACGCG GGCCGGCATT CACTACCCGC AGGAGAAGTT CGATCGGCTG 
ATTTCTCGAC AACACTATAT GCAGCAGACT TTAACGACGT CCCCGTCATC CACGGCTGGA 
ATGACCAGTG GAGTCTCCCC GTATCGCAGT CCTATGCGCT TGCAGCACCA GCAGCCTCAA 
CAGCAGCTGG AGAACAATAT ACCAAATTTG GCGGATGTTT TGCCAAAGCA CGATCCGCAG 
CTGGTAAAAG TTATAAAAGG TGTAAGCAGC ACGGACACTC TAAAGGCGCG CGCGGCTATT 
AACGAGCTTG CCGCCATCAT CGAGGCTCCA GAGAAGCAGG CTGTGCTGCG CGACTATGAA 
GAAATATTTA TTCAGAATGT GCTTGCACAG TTTAAGAATC TCTCACAAAT ACCATCGGCT 
CAATCCGTAG TTGTTTACCA GCCGCTATTA TCCATTCTGT ATACATTTTT CCATGCCAAC 
ATTCTGGGCA AAACGCTGAG CGTGGCCTGC ATCAAAAATC TAATGTCGGC GCTGTTGAAC 
CTTATGGCTG ATCCGAAGTT GGCCGTGGGT GATGATAGTC AGTATAACAA GGTTATTAAT 
GGCATATGTC TAAAAGTGTT GGACAAGGTA GATTTTACGA ATTTAAACTG TGCTTTGATA 
CGCCTATTAC GGGAGACTTG TCCGGAGGCA AAGCTGCCGA AGTTTACAGA CCTGCTGATG 
AAGTGCATAT GGCGCAACGT TAAGATGCTA CCCGAACGAA GCAACGAGCT GAACTATGAC 
GCCGTAATTC TCGAGGTGCA CGAGTTCATG CTCGCGCTGC CTAGCACTTG GTGGCAAAAC 
CGACCGTCGG ACACTCCAAT GCGCACGATT AAGACCATTC TGCATAACAT GGCAAAGGTG 
AAGGGGAATG CTATTCTGCA GCATCTCAAC CAGATTCCAA CACACTCGGA GCTACATACT 
TATTTGATAC GCATTCTCAA GAATTTCCAA AAGGATGGCT CTGCTTCAGG AATTGGTGCC 
TCCCCCCAGA GGGCCAAGGA GATTGCCTCC AAGCGAATTT CGCACCAAAC ACATGATACA 
GTATCACAGA TCTTTAAGCT GATCTCAGAT AGAGATACCA AACAGCAAGG TCTGCAGAAA 
CTTTACGACT TTAAGCAACA AAATCCTGAT ATCGATCTAA GTACCTTTCT GCAAGGCTCC 
AGTGCCCCAT TTCACAAGTA CATCGAGGAG GGTTTAGCCG AGATTGAGAG GAATCAGAAC 
GCTGGATCCA CACAGGATAA TCGGACGGCA GCTACTCGTT CTTATCTCAC AGATGTCAAC 
TACCAGAACA ACGGTCCCGA TCCCGATTTC TGGATGGACC GCCTGCAGTA TCATATGACC 
GGAGGGGCCG CCAAGCTAGC ATCTGCACGT TCAGCAGACG ATGGATCCCA TATGCTGGAC 
AATAAGGTGG TTGACGAGAA CCTCTGCCTG AACGGAATGA ACGCGCAAAA GGCGTCGCTC 
ATTAAGCGAG AAAAGCGGGA CATGTCACCA AATCGCTTGC AGCATCTCCA GGCAAAGCTG 
GCTCAAATCA AGAAAGAGAA TCATGCCCAA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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msps cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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