msps cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following msps gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the msps cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa05078 NM_169698.3
Latest version!
Drosophila melanogaster mini spindles (msps), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OFa08419 NM_001260200.2
Latest version!
Drosophila melanogaster mini spindles (msps), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OFa08420 NM_001260201.2
Latest version!
Drosophila melanogaster mini spindles (msps), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OFa08421 NM_001260202.1
Latest version!
Drosophila melanogaster mini spindles (msps), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OFa08421 NM_001170156.2
Latest version!
Drosophila melanogaster mini spindles (msps), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa05078
    Clone ID Related Accession (Same CDS sequence) NM_169698.3
    Accession Version NM_169698.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6129bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product mini spindles, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169698.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCGAGG ACACAGAGTA CAAGAAGTTG CCGGTGGAGG AGCGGTGTGT CCATAAACTA 
    TGGAAAGCCC GCGTCGATGG TTACGAGGAG GCCGCAAAGA TCTTTCGCGA GCTGGATGAC
    GAGAAGTCGC CGGAATGGTC TAAATTTGCA GGGCTCATAA AGAAGATGGT AGTGGACTCT
    AATGCTCTGG CCCAGGAAAA GGGTCTAGAA GCGGCCCTCA TTTTTGTGGA GAACAGCGGG
    CTGGCGGGAC GCACAGTAGG CGACGTGATG ACAGGCATCG TTCAGAAGTG CATTGCGGCG
    CCAAAGACCA AAACCAAGGA GCTCTCCGTC CAAGTGGCTC TAATGTATGT GGAGATAGAG
    AAGCAGGAGG CCGTCGTCGA AGAGCTGGTT AAAGGTATGG AGGCCAAGAA CCCTAAGATT
    GTGTCTGCCT GCGTGGCAGC CACCACACTG GCCCTCCGCG AGTTCGGACA CAAGGTGATC
    GGCGTCAAGC CGTTAATTAA AAAGCTTGCT CCGCTAATGT CTGACCGAGA CAAGACGGTC
    CGAGATGAGG GCAAGCAGCT GGCTGTTGAG ATTTACCGTT GGATCGGAGC TGCAATGAAG
    GCACAGATCT CAACGCTACC ACAGGTAACG CTAAAGGAGC TGGAGGATGA ATTCGACAAA
    CTCAAAGGTG AACGCGTTGA ACCCTCCAGA TATCTAAAGT CCCAGCAGGA AAAGCAAGCG
    AAGATCGCCG ATGCTGCAGC GACCGAGGAT GCATATAATG AGGATGATGG CGAGGCTGGC
    GTTGAAGAAA TCGATCCTAT GGATCTTCTA GACCCCGTTG ACATTCTCTC CAAAATGCCC
    AAGGACTTCT ACGATAAGCT GGAGGAAAAG AAATGGACTC TGCGCAAAGA ATCGTTGGAA
    GTCCTGGAGA AACTGCTCAC TGACCACCCC AAGCTAGAGA ATGGCGAGTA TGGAGCGCTG
    GTAAGCGCTC TTAAAAAGGT TATAACCAAG GACTCCAACG TAGTCCTCGT TGCAATGGCC
    GGAAAATGTC TAGCCTTGCT AGCCAAAGGA TTGGCAAAGC GATTCTCAAA TTACGCATCC
    GCTTGTGTCC CATCCCTGTT GGAAAAATTT AAAGAGAAGA AACCTAATGT TGTGACAGCG
    CTGCGTGAAG CTATTGATGC CATATACGCC TCCACTTCTC TAGAGGCCCA GCAAGAGTCC
    ATTGTAGAGT CCCTGTCCAA CAAAAATCCA AGTGTTAAGT CCGAAACGGC CTTGTTTATT
    GCCCGTGCCC TAACCCGCAC CCAACCAACC GCGCTAAACA AAAAGCTGCT TAAACTTCTG
    ACCACCAGTC TAGTGAAAAC GCTTAACGAA CCAGATCCAA CCGTACGCGA CAGCAGCGCC
    GAAGCTCTTG GTACTTTAAT AAAGCTAATG GGAGACAAAG CGGTGACTCC TTTGCTCGCC
    GACGTCGATC CACTCAAAAT GGCCAAGATA AAGGAGTGCC AAGAAAAGGC GGAGATTAAA
    ATAAAGGTTG CAGGTCCAAA GAAGGAGACA CGACCTGCAT CTGCACCGAC TGCGAAAGCT
    GCTGCTCCGG CTAAGACAGT GGCTGGTAGT GTGGACCCTA AGCCAGTAAC CCGACCGGCC
    ACTACAGGGG CACGTAAGGT CTTGAAAAAA CCGGCGACGG TTAGTGGAGG AGGAGCTACA
    TCTGCCCCAA CTGCAGCTCT TAAAGCGGGT GGAAAACCCC TAGCCACGGA ACGCGAAATA
    ACCCCCGAGG AATTGCAAGA AAAATCCGAA GAAATTCTGC CAGCCGAAAT TCTTAATGGG
    CTGGTGGATT CCAACTGGAA GAACCGCCTT GCTGCTGTGG AACAGCTTCT GGGCGAAATA
    AGCGGTTTTG ATGCAAAACA GGCTGGCATA TCACAGATCC TGATCCGAAC AATCAGTGGA
    CGAAAGCCAG GCCTCAAAGA AATGAATTTC CAGGTACTCA AGTTTAAGCT AGACATAATC
    CGCAGTGTGG CTGAAAACTA TCCACTGACC ACGACAACCG TTGATCTGGT GATTAACGAA
    ATAATCGAGA AGCTAGCAGA CGCCAAGAAT GGTGCAGCAG CTGCAGATGT TTTGTCTGCC
    TTTGCCGAAG CCACTAAACT GGAGTATGTT GTGGGCAAAG TATTGAGCTT TGCTTTTGAA
    CAAAAATCGC CTAAAGTGCA ATCAGAGGCC TTTAACTGGG TTAACAGGTC AATCATAGAA
    TTCGGCTTCC AATTGCAGCC AAAGACACTT ATTGAAGATG TACGAAAAGG CGTGCAGAGC
    ACGAATCCAA CGGTTCGTGC TTCCGCCATT CAAATGGTAG GCACCATGTC TATGTATATG
    GGCAAAGCTC TAATGATGTT CTTCGACAGC GAGAAGCCTG CACTAAAGTC CCAGATCCAG
    GTAGAGTTTG ACAAGAATGT GGGCGAAAAA CCACCCAAGC CTGTTAGAGG AGTTCAACGT
    AGCAGCGGTG GAACCGCTGG TAATTCCCCA GACAATGAGG ACGATGATGG TGGGGCAGCC
    GGAGAAGAGG AGCCCATTAA TATGGCTGAT CTTCTACCAC GCGTTGATAT TGCTCCACAG
    ATTACGGAGG CATTGCTGAA GGAGATGTCC GACAAGGACT GGAAAACTCG CAACGAGGGT
    CTGACTAAGC TGCAGGCTAT TATTTCAGAG GCGCGGCTAA TCAAACCCAG CATTGGCGAC
    TTGGCTCCTG CTCTGGCCCA CCGTCTGGTA GATTCCAATG CTAAGATTGC CCAAACGACA
    CTTGCCATTT GCGAGCAGCT TGCCACAGCC ATGGGGGCTG GTTGTCGAAA TCACGTGCGG
    AACTTGTTCC CTGGCTTTTT ACACGCTCTG GGTGATAACA AGAGTTTTGT AAGAGCCGCT
    GCCCTCAATT GCATCAATAG TTTTGGCGAA AAGGGCGGCT ATAAGGAATT TTTTGAGAGC
    GAAATGATAG CCGATGCCCT GAAAGGAGGA TCCCCAGCTC TAAAGACAGA ATTGTGGGCT
    TGGCTGGCAG ATAAGCTGCC TGGTCTTCCC CCCAAGTCAG TATCGAAGGA GGATATCCAT
    TCAATGGTGC CGCACTTATA TGCTCACATC TGCGATCGAA ACGCCGATGT CCGGAAAAAT
    GCCAACGAGG CGGTGTTGGG CATTATGATT CACCTTGGAT TTGATGCAAT GAACCGCGCT
    CTGGACAAAC AGAAGCCCGC GTCCAAAAAG GATATACTTG CAGCACTCGA AAAAGCGCGT
    CCTAATCTTC CGGTAAAACC GCTGCCCAAA GGCAAGCATC AGGCTCCAAT ACCTGAGGAG
    CCGAAGCTAA AAACAGTGCG CGGCGGTGGA GCAGGTGGTG CGCCTGGAAT CCAGAAGAGT
    GCTACAGCCC GGGTTGCCGG AGGACAAGAC AAGCAGGTAC CAGCGCGCAA AAAGGACGAA
    GATATCGACA CATCGCCGCT ACTGTGCGCC AACAGTGCTA AAAACCAGCG GCTGCTAGAC
    GAGCAAAAAA TGAAGGTACT AAAGTGGACT TTTGTAACCC CAAGAGAGGA ATTCACCGAA
    CTGTTACGCG ACCAAATGAT GACCGCAAAC GTAAATAAAG CACTGATAGC CAACATGTTC
    CACGACGATT TTCGCTACCA CTTGAAAGTC ATCGAGCAGT TGAGCGAAGA TCTGGCTGGT
    AACAGTAAGG CGCTGGTGTG TAATCTGGAC CTAATACTTA AATGGCTGAC GCTGCGATTT
    TACGATACGA ACCCTTCTGT GTTGATTAAG GGTCTCGAAT ATCTAGTACA GGTTTTCCAG
    GTACTCATTG ACGAGGAATA CATTCTGGCC GAGAACGAGG GAAGCAGTTT TGTCCCACAT
    CTGCTTTTAA AGATCGGAGA TCCAAAGGAT GCTGTTCGAA ATGGCGTACG CCGCGTTCTG
    CGGCAAGTAA TATTGGTCTT CCCTTTCGTC AAGGTCTTTG GCTATGTGAT GGAGGGTCTA
    AAATCGAAGA ATGCCCGTCA GCGTACTGAG TGCCTTGACG AGTTAACCTT CCTAATTGAA
    AGCTATGGCA TGAACATCTG TCCCCAATCG GCGGTGCGCG AGATTGCTCG CCAGATTTCC
    GACCGGGATA ATTCCGTACG CAACGCAGCA CTCAACTGCA TCGTCCAGGT CTTCTTTTTG
    TCCGGGGAAA AGACTTACAA GATGATTGGC CATCTTAACG AAAAGGACCT CTCAATGCTA
    GATGAGCGCA TCAAGCGAGC CAAAAAGACT AAGAAGCCCA CGCCACCGCC ATCTGTTGAT
    GTGCCGGCAC CTCAACGACA TGACAGCATC GAAATCGAAG ATGCTGAAGT GGGCAACGGT
    TGCGACGAGC TTCCACCACC AGATGAGGAC GGAACTTTCG ACCAGGCACC TTCGTCACAG
    CTGCTTCTAC TCCAGCAACA GCTTCAACAG TTACAACAGC AGGCCCAGCA ACAGAAGCCC
    AGCGGACCAT TCGGCTTGGA CTCGCAGGTA ATCAGCGAGA TCGAAAAAGA TTGGGTCCGA
    GTGGACCAAA TGGAGCAGAA GCCGCTACTG AACGTAGATA TCTCATCGCT GGACGAGCCT
    ATTAAGGTGA GGCCCACGCG GGCCGGCATT CACTACCCGC AGGAGAAGTT CGATCGGCTG
    ATTTCTCGAC AACACTATAT GCAGCAGACT TTAACGACGT CCCCGTCATC CACGGCTGGA
    ATGACCAGTG GAGTCTCCCC GTATCGCAGT CCTATGCGCT TGCAGCACCA GCAGCCTCAA
    CAGCAGCTGG AGAACAATAT ACCAAATTTG GCGGATGTTT TGCCAAAGCA CGATCCGCAG
    CTGGTAAAAG TTATAAAAGG TGTAAGCAGC ACGGACACTC TAAAGGCGCG CGCGGCTATT
    AACGAGCTTG CCGCCATCAT CGAGGCTCCA GAGAAGCAGG CTGTGCTGCG CGACTATGAA
    GAAATATTTA TTCAGAATGT GCTTGCACAG TTTAAGAATC TCTCACAAAT ACCATCGGCT
    CAATCCGTAG TTGTTTACCA GCCGCTATTA TCCATTCTGT ATACATTTTT CCATGCCAAC
    ATTCTGGGCA AAACGCTGAG CGTGGCCTGC ATCAAAAATC TAATGTCGGC GCTGTTGAAC
    CTTATGGCTG ATCCGAAGTT GGCCGTGGGT GATGATAGTC AGTATAACAA GGTTATTAAT
    GGCATATGTC TAAAAGTGTT GGACAAGGTA GATTTTACGA ATTTAAACTG TGCTTTGATA
    CGCCTATTAC GGGAGACTTG TCCGGAGGCA AAGCTGCCGA AGTTTACAGA CCTGCTGATG
    AAGTGCATAT GGCGCAACGT TAAGATGCTA CCCGAACGAA GCAACGAGCT GAACTATGAC
    GCCGTAATTC TCGAGGTGCA CGAGTTCATG CTCGCGCTGC CTAGCACTTG GTGGCAAAAC
    CGACCGTCGG ACACTCCAAT GCGCACGATT AAGACCATTC TGCATAACAT GGCAAAGGTG
    AAGGGGAATG CTATTCTGCA GCATCTCAAC CAGATTCCAA CACACTCGGA GCTACATACT
    TATTTGATAC GCATTCTCAA GAATTTCCAA AAGGATGGCT CTGCTTCAGG AATTGGTGCC
    TCCCCCCAGA GGGCCAAGGA GATTGCCTCC AAGCGAATTT CGCACCAAAC ACATGATACA
    GTATCACAGA TCTTTAAGCT GATCTCAGAT AGAGATACCA AACAGCAAGG TCTGCAGAAA
    CTTTACGACT TTAAGCAACA AAATCCTGAT ATCGATCTAA GTACCTTTCT GCAAGGCTCC
    AGTGCCCCAT TTCACAAGTA CATCGAGGAG GGTTTAGCCG AGATTGAGAG GAATCAGAAC
    GCTGGATCCA CACAGGATAA TCGGACGGAT GTCAACTACC AGAACAACGG TCCCGATCCC
    GATTTCTGGA TGGACCGCCT GCAGTATCAT ATGACCGGAG GGGCCGCCAA GCTAGCATCT
    GCACGTTCAG CAGACGATGG ATCCCATATG CTGGACAATA AGGTGGTTGA CGAGAACCTC
    TGCCTGAACG GAATGAACGC GCAAAAGGCG TCGCTCATTA AGCGAGAAAA GCGGGACATG
    TCACCAAATC GCTTGCAGCA TCTCCAGGCA AAGCTGGCTC AAATCAAGAA AGAGAATCAT
    GCCCAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_732105.2
    CDS447..6575
    Translation

    Target ORF information:

    RefSeq Version NM_169698.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster mini spindles (msps), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_169698.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCGAGG ACACAGAGTA CAAGAAGTTG CCGGTGGAGG AGCGGTGTGT CCATAAACTA 
    TGGAAAGCCC GCGTCGATGG TTACGAGGAG GCCGCAAAGA TCTTTCGCGA GCTGGATGAC
    GAGAAGTCGC CGGAATGGTC TAAATTTGCA GGGCTCATAA AGAAGATGGT AGTGGACTCT
    AATGCTCTGG CCCAGGAAAA GGGTCTAGAA GCGGCCCTCA TTTTTGTGGA GAACAGCGGG
    CTGGCGGGAC GCACAGTAGG CGACGTGATG ACAGGCATCG TTCAGAAGTG CATTGCGGCG
    CCAAAGACCA AAACCAAGGA GCTCTCCGTC CAAGTGGCTC TAATGTATGT GGAGATAGAG
    AAGCAGGAGG CCGTCGTCGA AGAGCTGGTT AAAGGTATGG AGGCCAAGAA CCCTAAGATT
    GTGTCTGCCT GCGTGGCAGC CACCACACTG GCCCTCCGCG AGTTCGGACA CAAGGTGATC
    GGCGTCAAGC CGTTAATTAA AAAGCTTGCT CCGCTAATGT CTGACCGAGA CAAGACGGTC
    CGAGATGAGG GCAAGCAGCT GGCTGTTGAG ATTTACCGTT GGATCGGAGC TGCAATGAAG
    GCACAGATCT CAACGCTACC ACAGGTAACG CTAAAGGAGC TGGAGGATGA ATTCGACAAA
    CTCAAAGGTG AACGCGTTGA ACCCTCCAGA TATCTAAAGT CCCAGCAGGA AAAGCAAGCG
    AAGATCGCCG ATGCTGCAGC GACCGAGGAT GCATATAATG AGGATGATGG CGAGGCTGGC
    GTTGAAGAAA TCGATCCTAT GGATCTTCTA GACCCCGTTG ACATTCTCTC CAAAATGCCC
    AAGGACTTCT ACGATAAGCT GGAGGAAAAG AAATGGACTC TGCGCAAAGA ATCGTTGGAA
    GTCCTGGAGA AACTGCTCAC TGACCACCCC AAGCTAGAGA ATGGCGAGTA TGGAGCGCTG
    GTAAGCGCTC TTAAAAAGGT TATAACCAAG GACTCCAACG TAGTCCTCGT TGCAATGGCC
    GGAAAATGTC TAGCCTTGCT AGCCAAAGGA TTGGCAAAGC GATTCTCAAA TTACGCATCC
    GCTTGTGTCC CATCCCTGTT GGAAAAATTT AAAGAGAAGA AACCTAATGT TGTGACAGCG
    CTGCGTGAAG CTATTGATGC CATATACGCC TCCACTTCTC TAGAGGCCCA GCAAGAGTCC
    ATTGTAGAGT CCCTGTCCAA CAAAAATCCA AGTGTTAAGT CCGAAACGGC CTTGTTTATT
    GCCCGTGCCC TAACCCGCAC CCAACCAACC GCGCTAAACA AAAAGCTGCT TAAACTTCTG
    ACCACCAGTC TAGTGAAAAC GCTTAACGAA CCAGATCCAA CCGTACGCGA CAGCAGCGCC
    GAAGCTCTTG GTACTTTAAT AAAGCTAATG GGAGACAAAG CGGTGACTCC TTTGCTCGCC
    GACGTCGATC CACTCAAAAT GGCCAAGATA AAGGAGTGCC AAGAAAAGGC GGAGATTAAA
    ATAAAGGTTG CAGGTCCAAA GAAGGAGACA CGACCTGCAT CTGCACCGAC TGCGAAAGCT
    GCTGCTCCGG CTAAGACAGT GGCTGGTAGT GTGGACCCTA AGCCAGTAAC CCGACCGGCC
    ACTACAGGGG CACGTAAGGT CTTGAAAAAA CCGGCGACGG TTAGTGGAGG AGGAGCTACA
    TCTGCCCCAA CTGCAGCTCT TAAAGCGGGT GGAAAACCCC TAGCCACGGA ACGCGAAATA
    ACCCCCGAGG AATTGCAAGA AAAATCCGAA GAAATTCTGC CAGCCGAAAT TCTTAATGGG
    CTGGTGGATT CCAACTGGAA GAACCGCCTT GCTGCTGTGG AACAGCTTCT GGGCGAAATA
    AGCGGTTTTG ATGCAAAACA GGCTGGCATA TCACAGATCC TGATCCGAAC AATCAGTGGA
    CGAAAGCCAG GCCTCAAAGA AATGAATTTC CAGGTACTCA AGTTTAAGCT AGACATAATC
    CGCAGTGTGG CTGAAAACTA TCCACTGACC ACGACAACCG TTGATCTGGT GATTAACGAA
    ATAATCGAGA AGCTAGCAGA CGCCAAGAAT GGTGCAGCAG CTGCAGATGT TTTGTCTGCC
    TTTGCCGAAG CCACTAAACT GGAGTATGTT GTGGGCAAAG TATTGAGCTT TGCTTTTGAA
    CAAAAATCGC CTAAAGTGCA ATCAGAGGCC TTTAACTGGG TTAACAGGTC AATCATAGAA
    TTCGGCTTCC AATTGCAGCC AAAGACACTT ATTGAAGATG TACGAAAAGG CGTGCAGAGC
    ACGAATCCAA CGGTTCGTGC TTCCGCCATT CAAATGGTAG GCACCATGTC TATGTATATG
    GGCAAAGCTC TAATGATGTT CTTCGACAGC GAGAAGCCTG CACTAAAGTC CCAGATCCAG
    GTAGAGTTTG ACAAGAATGT GGGCGAAAAA CCACCCAAGC CTGTTAGAGG AGTTCAACGT
    AGCAGCGGTG GAACCGCTGG TAATTCCCCA GACAATGAGG ACGATGATGG TGGGGCAGCC
    GGAGAAGAGG AGCCCATTAA TATGGCTGAT CTTCTACCAC GCGTTGATAT TGCTCCACAG
    ATTACGGAGG CATTGCTGAA GGAGATGTCC GACAAGGACT GGAAAACTCG CAACGAGGGT
    CTGACTAAGC TGCAGGCTAT TATTTCAGAG GCGCGGCTAA TCAAACCCAG CATTGGCGAC
    TTGGCTCCTG CTCTGGCCCA CCGTCTGGTA GATTCCAATG CTAAGATTGC CCAAACGACA
    CTTGCCATTT GCGAGCAGCT TGCCACAGCC ATGGGGGCTG GTTGTCGAAA TCACGTGCGG
    AACTTGTTCC CTGGCTTTTT ACACGCTCTG GGTGATAACA AGAGTTTTGT AAGAGCCGCT
    GCCCTCAATT GCATCAATAG TTTTGGCGAA AAGGGCGGCT ATAAGGAATT TTTTGAGAGC
    GAAATGATAG CCGATGCCCT GAAAGGAGGA TCCCCAGCTC TAAAGACAGA ATTGTGGGCT
    TGGCTGGCAG ATAAGCTGCC TGGTCTTCCC CCCAAGTCAG TATCGAAGGA GGATATCCAT
    TCAATGGTGC CGCACTTATA TGCTCACATC TGCGATCGAA ACGCCGATGT CCGGAAAAAT
    GCCAACGAGG CGGTGTTGGG CATTATGATT CACCTTGGAT TTGATGCAAT GAACCGCGCT
    CTGGACAAAC AGAAGCCCGC GTCCAAAAAG GATATACTTG CAGCACTCGA AAAAGCGCGT
    CCTAATCTTC CGGTAAAACC GCTGCCCAAA GGCAAGCATC AGGCTCCAAT ACCTGAGGAG
    CCGAAGCTAA AAACAGTGCG CGGCGGTGGA GCAGGTGGTG CGCCTGGAAT CCAGAAGAGT
    GCTACAGCCC GGGTTGCCGG AGGACAAGAC AAGCAGGTAC CAGCGCGCAA AAAGGACGAA
    GATATCGACA CATCGCCGCT ACTGTGCGCC AACAGTGCTA AAAACCAGCG GCTGCTAGAC
    GAGCAAAAAA TGAAGGTACT AAAGTGGACT TTTGTAACCC CAAGAGAGGA ATTCACCGAA
    CTGTTACGCG ACCAAATGAT GACCGCAAAC GTAAATAAAG CACTGATAGC CAACATGTTC
    CACGACGATT TTCGCTACCA CTTGAAAGTC ATCGAGCAGT TGAGCGAAGA TCTGGCTGGT
    AACAGTAAGG CGCTGGTGTG TAATCTGGAC CTAATACTTA AATGGCTGAC GCTGCGATTT
    TACGATACGA ACCCTTCTGT GTTGATTAAG GGTCTCGAAT ATCTAGTACA GGTTTTCCAG
    GTACTCATTG ACGAGGAATA CATTCTGGCC GAGAACGAGG GAAGCAGTTT TGTCCCACAT
    CTGCTTTTAA AGATCGGAGA TCCAAAGGAT GCTGTTCGAA ATGGCGTACG CCGCGTTCTG
    CGGCAAGTAA TATTGGTCTT CCCTTTCGTC AAGGTCTTTG GCTATGTGAT GGAGGGTCTA
    AAATCGAAGA ATGCCCGTCA GCGTACTGAG TGCCTTGACG AGTTAACCTT CCTAATTGAA
    AGCTATGGCA TGAACATCTG TCCCCAATCG GCGGTGCGCG AGATTGCTCG CCAGATTTCC
    GACCGGGATA ATTCCGTACG CAACGCAGCA CTCAACTGCA TCGTCCAGGT CTTCTTTTTG
    TCCGGGGAAA AGACTTACAA GATGATTGGC CATCTTAACG AAAAGGACCT CTCAATGCTA
    GATGAGCGCA TCAAGCGAGC CAAAAAGACT AAGAAGCCCA CGCCACCGCC ATCTGTTGAT
    GTGCCGGCAC CTCAACGACA TGACAGCATC GAAATCGAAG ATGCTGAAGT GGGCAACGGT
    TGCGACGAGC TTCCACCACC AGATGAGGAC GGAACTTTCG ACCAGGCACC TTCGTCACAG
    CTGCTTCTAC TCCAGCAACA GCTTCAACAG TTACAACAGC AGGCCCAGCA ACAGAAGCCC
    AGCGGACCAT TCGGCTTGGA CTCGCAGGTA ATCAGCGAGA TCGAAAAAGA TTGGGTCCGA
    GTGGACCAAA TGGAGCAGAA GCCGCTACTG AACGTAGATA TCTCATCGCT GGACGAGCCT
    ATTAAGGTGA GGCCCACGCG GGCCGGCATT CACTACCCGC AGGAGAAGTT CGATCGGCTG
    ATTTCTCGAC AACACTATAT GCAGCAGACT TTAACGACGT CCCCGTCATC CACGGCTGGA
    ATGACCAGTG GAGTCTCCCC GTATCGCAGT CCTATGCGCT TGCAGCACCA GCAGCCTCAA
    CAGCAGCTGG AGAACAATAT ACCAAATTTG GCGGATGTTT TGCCAAAGCA CGATCCGCAG
    CTGGTAAAAG TTATAAAAGG TGTAAGCAGC ACGGACACTC TAAAGGCGCG CGCGGCTATT
    AACGAGCTTG CCGCCATCAT CGAGGCTCCA GAGAAGCAGG CTGTGCTGCG CGACTATGAA
    GAAATATTTA TTCAGAATGT GCTTGCACAG TTTAAGAATC TCTCACAAAT ACCATCGGCT
    CAATCCGTAG TTGTTTACCA GCCGCTATTA TCCATTCTGT ATACATTTTT CCATGCCAAC
    ATTCTGGGCA AAACGCTGAG CGTGGCCTGC ATCAAAAATC TAATGTCGGC GCTGTTGAAC
    CTTATGGCTG ATCCGAAGTT GGCCGTGGGT GATGATAGTC AGTATAACAA GGTTATTAAT
    GGCATATGTC TAAAAGTGTT GGACAAGGTA GATTTTACGA ATTTAAACTG TGCTTTGATA
    CGCCTATTAC GGGAGACTTG TCCGGAGGCA AAGCTGCCGA AGTTTACAGA CCTGCTGATG
    AAGTGCATAT GGCGCAACGT TAAGATGCTA CCCGAACGAA GCAACGAGCT GAACTATGAC
    GCCGTAATTC TCGAGGTGCA CGAGTTCATG CTCGCGCTGC CTAGCACTTG GTGGCAAAAC
    CGACCGTCGG ACACTCCAAT GCGCACGATT AAGACCATTC TGCATAACAT GGCAAAGGTG
    AAGGGGAATG CTATTCTGCA GCATCTCAAC CAGATTCCAA CACACTCGGA GCTACATACT
    TATTTGATAC GCATTCTCAA GAATTTCCAA AAGGATGGCT CTGCTTCAGG AATTGGTGCC
    TCCCCCCAGA GGGCCAAGGA GATTGCCTCC AAGCGAATTT CGCACCAAAC ACATGATACA
    GTATCACAGA TCTTTAAGCT GATCTCAGAT AGAGATACCA AACAGCAAGG TCTGCAGAAA
    CTTTACGACT TTAAGCAACA AAATCCTGAT ATCGATCTAA GTACCTTTCT GCAAGGCTCC
    AGTGCCCCAT TTCACAAGTA CATCGAGGAG GGTTTAGCCG AGATTGAGAG GAATCAGAAC
    GCTGGATCCA CACAGGATAA TCGGACGGAT GTCAACTACC AGAACAACGG TCCCGATCCC
    GATTTCTGGA TGGACCGCCT GCAGTATCAT ATGACCGGAG GGGCCGCCAA GCTAGCATCT
    GCACGTTCAG CAGACGATGG ATCCCATATG CTGGACAATA AGGTGGTTGA CGAGAACCTC
    TGCCTGAACG GAATGAACGC GCAAAAGGCG TCGCTCATTA AGCGAGAAAA GCGGGACATG
    TCACCAAATC GCTTGCAGCA TCTCCAGGCA AAGCTGGCTC AAATCAAGAA AGAGAATCAT
    GCCCAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08419
    Clone ID Related Accession (Same CDS sequence) NM_001260200.2
    Accession Version NM_001260200.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product mini spindles, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260200.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCCGAGG ACACAGAGTA CAAGAAGTTG CCGGTGGAGG AGCGGTGTGT CCATAAACTA 
    TGGAAAGCCC GCGTCGATGG TTACGAGGAG GCCGCAAAGA TCTTTCGCGA GCTGGATGAC
    GAGAAGTCGC CGGAATGGTC TAAATTTGCA GGGCTCATAA AGAAGATGGT AGTGGACTCT
    AATGCTCTGG CCCAGGAAAA GGGTCTAGAA GCGGCCCTCA TTTTTGTGGA GAACAGCGGG
    CTGGCGGGAC GCACAGTAGG CGACGTGATG ACAGGCATCG TTCAGAAGTG CATTGCGGCG
    CCAAAGACCA AAACCAAGGA GCTCTCCGTC CAAGTGGCTC TAATGTATGT GGAGATAGAG
    AAGCAGGAGG CCGTCGTCGA AGAGCTGGTT AAAGGTATGG AGGCCAAGAA CCCTAAGATT
    GTGTCTGCCT GCGTGGCAGC CACCACACTG GCCCTCCGCG AGTTCGGACA CAAGGTGATC
    GGCGTCAAGC CGTTAATTAA AAAGCTTGCT CCGCTAATGT CTGACCGAGA CAAGACGGTC
    CGAGATGAGG GCAAGCAGCT GGCTGTTGAG ATTTACCGTT GGATCGGAGC TGCAATGAAG
    GCACAGATCT CAACGCTACC ACAGGTAACG CTAAAGGAGC TGGAGGATGA ATTCGACAAA
    CTCAAAGGTG AACGCGTTGA ACCCTCCAGA TATCTAAAGT CCCAGCAGGA AAAGCAAGCG
    AAGATCGCCG ATGCTGCAGC GACCGAGGAT GCATATAATG AGGATGATGG CGAGGCTGGC
    GTTGAAGAAA TCGATCCTAT GGATCTTCTA GACCCCGTTG ACATTCTCTC CAAAATGCCC
    AAGGACTTCT ACGATAAGCT GGAGGAAAAG AAATGGACTC TGCGCAAAGA ATCGTTGGAA
    GTCCTGGAGA AACTGCTCAC TGACCACCCC AAGCTAGAGA ATGGCGAGTA TGGAGCGCTG
    GTAAGCGCTC TTAAAAAGGT TATAACCAAG GACTCCAACG TAGTCCTCGT TGCAATGGCC
    GGAAAATGTC TAGCCTTGCT AGCCAAAGGA TTGGCAAAGC GATTCTCAAA TTACGCATCC
    GCTTGTGTCC CATCCCTGTT GGAAAAATTT AAAGAGAAGA AACCTAATGT TGTGACAGCG
    CTGCGTGAAG CTATTGATGC CATATACGCC TCCACTTCTC TAGAGGCCCA GCAAGAGTCC
    ATTGTAGAGT CCCTGTCCAA CAAAAATCCA AGTGTTAAGT CCGAAACGGC CTTGTTTATT
    GCCCGTGCCC TAACCCGCAC CCAACCAACC GCGCTAAACA AAAAGCTGCT TAAACTTCTG
    ACCACCAGTC TAGTGAAAAC GCTTAACGAA CCAGATCCAA CCGTACGCGA CAGCAGCGCC
    GAAGCTCTTG GTACTTTAAT AAAGCTAATG GGAGACAAAG CGGTGACTCC TTTGCTCGCC
    GACGTCGATC CACTCAAAAT GGCCAAGATA AAGGAGTGCC AAGAAAAGGC GGAGATTAAA
    ATAAAGGTTG CAGGTCCAAA GAAGGAGACA CGACCTGCAT CTGCACCGAC TGCGAAAGCT
    GCTGCTCCGG CTAAGACAGT GGCTGGTAGT GTGGACCCTA AGCCAGTAAC CCGACCGGCC
    ACTACAGGGG CACGTAAGGT CTTGAAAAAA CCGGCGACGG TTAGTGGAGG AGGAGCTACA
    TCTGCCCCAA CTGCAGCTCT TAAAGCGGGT GGAAAACCCC TAGCCACGGA ACGCGAAATA
    ACCCCCGAGG AATTGCAAGA AAAATCCGAA GAAATTCTGC CAGCCGAAAT TCTTAATGGG
    CTGGTGGATT CCAACTGGAA GAACCGCCTT GCTGCTGTGG AACAGCTTCT GGGCGAAATA
    AGCGGTTTTG ATGCAAAACA GGCTGGCATA TCACAGATCC TGATCCGAAC AATCAGTGGA
    CGAAAGCCAG GCCTCAAAGA AATGAATTTC CAGGTACTCA AGTTTAAGCT AGACATAATC
    CGCAGTGTGG CTGAAAACTA TCCACTGACC ACGACAACCG TTGATCTGGT GATTAACGAA
    ATAATCGAGA AGCTAGCAGA CGCCAAGAAT GGTGCAGCAG CTGCAGATGT TTTGTCTGCC
    TTTGCCGAAG CCACTAAACT GGAGTATGTT GTGGGCAAAG TATTGAGCTT TGCTTTTGAA
    CAAAAATCGC CTAAAGTGCA ATCAGAGGCC TTTAACTGGG TTAACAGGTC AATCATAGAA
    TTCGGCTTCC AATTGCAGCC AAAGACACTT ATTGAAGATG TACGAAAAGG CGTGCAGAGC
    ACGAATCCAA CGGTTCGTGC TTCCGCCATT CAAATGGTAG GCACCATGTC TATGTATATG
    GGCAAAGCTC TAATGATGTT CTTCGACAGC GAGAAGCCTG CACTAAAGTC CCAGATCCAG
    GTAGAGTTTG ACAAGAATGT GGGCGAAAAA CCACCCAAGC CTGTTAGAGG AGTTCAACGT
    AGCAGCGGTG GAACCGCTGG TAATTCCCCA GACAATGAGG ACGATGATGG TGGGGCAGCC
    GGAGAAGAGG AGCCCATTAA TATGGCTGAT CTTCTACCAC GCGTTGATAT TGCTCCACAG
    ATTACGGAGG CATTGCTGAA GGAGATGTCC GACAAGGACT GGAAAACTCG CAACGAGGGT
    CTGACTAAGC TGCAGGCTAT TATTTCAGAG GCGCGGCTAA TCAAACCCAG CATTGGCGAC
    TTGGCTCCTG CTCTGGCCCA CCGTCTGGTA GATTCCAATG CTAAGATTGC CCAAACGACA
    CTTGCCATTT GCGAGCAGCT TGCCACAGCC ATGGGGGCTG GTTGTCGAAA TCACGTGCGG
    AACTTGTTCC CTGGCTTTTT ACACGCTCTG GGTGATAACA AGAGTTTTGT AAGAGCCGCT
    GCCCTCAATT GCATCAATAG TTTTGGCGAA AAGGGCGGCT ATAAGGAATT TTTTGAGAGC
    GAAATGATAG CCGATGCCCT GAAAGGAGGA TCCCCAGCTC TAAAGACAGA ATTGTGGGCT
    TGGCTGGCAG ATAAGCTGCC TGGTCTTCCC CCCAAGTCAG TATCGAAGGA GGATATCCAT
    TCAATGGTGC CGCACTTATA TGCTCACATC TGCGATCGAA ACGCCGATGT CCGGAAAAAT
    GCCAACGAGG CGGTGTTGGG CATTATGATT CACCTTGGAT TTGATGCAAT GAACCGCGCT
    CTGGACAAAC AGAAGCCCGC GTCCAAAAAG GATATACTTG CAGCACTCGA AAAAGCGCGT
    CCTAATCTTC CGGTAAAACC GCTGCCCAAA GGCAAGCATC AGGCTCCAAT ACCTGAGGAG
    CCGAAGCTAA AAACAGTGCG CGGCGGTGGA GCAGGTGGTG CGCCTGGAAT CCAGAAGAGT
    GCTACAGCCC GGGTTGCCGG AGGACAAGAC AAGCAGGTAC CAGCGCGCAA AAAGGACGAA
    GATATCGACA CATCGCCGCT ACTGTGCGCC AACAGTGCTA AAAACCAGCG GCTGCTAGAC
    GAGCAAAAAA TGAAGGTACT AAAGTGGACT TTTGTAACCC CAAGAGAGGA ATTCACCGAA
    CTGTTACGCG ACCAAATGAT GACCGCAAAC GTAAATAAAG CACTGATAGC CAACATGTTC
    CACGACGATT TTCGCTACCA CTTGAAAGTC ATCGAGCAGT TGAGCGAAGA TCTGGCTGGT
    AACAGTAAGG CGCTGGTGTG TAATCTGGAC CTAATACTTA AATGGCTGAC GCTGCGATTT
    TACGATACGA ACCCTTCTGT GTTGATTAAG GGTCTCGAAT ATCTAGTACA GGTTTTCCAG
    GTACTCATTG ACGAGGAATA CATTCTGGCC GAGAACGAGG GAAGCAGTTT TGTCCCACAT
    CTGCTTTTAA AGATCGGAGA TCCAAAGGAT GCTGTTCGAA ATGGCGTACG CCGCGTTCTG
    CGGCAAGTAA TATTGGTCTT CCCTTTCGTC AAGGTCTTTG GCTATGTGAT GGAGGGTCTA
    AAATCGAAGA ATGCCCGTCA GCGTACTGAG TGCCTTGACG AGTTAACCTT CCTAATTGAA
    AGCTATGGCA TGAACATCTG TCCCCAATCG GCGGTGCGCG AGATTGCTCG CCAGATTTCC
    GACCGGGATA ATTCCGTACG CAACGCAGCA CTCAACTGCA TCGTCCAGGT CTTCTTTTTG
    TCCGGGGAAA AGACTTACAA GATGATTGGC CATCTTAACG AAAAGGACCT CTCAATGCTA
    GATGAGCGCA TCAAGCGAGC CAAAAAGACT AAGAAGCCCA CGCCACCGCC ATCTGTTGAT
    GTGCCGGCAC CTCAACGACA TGACAGCATC GAAATCGAAG ATGCTGAAGT GGGCAACGGT
    TGCGACGAGC TTCCACCACC AGATGAGGAC GGTTTGCAGC GAGAACTAAA TGCGCGTGGG
    CAGAACAACA TGACAGATCG GAGCGCCACA AACGTGGTTG CCTACTTGAG CTCCCTGACT
    CGACAGGCAA CTTTCGACCA GGCACCTTCG TCACAGCTGC TTCTACTCCA GCAACAGCTT
    CAACAGTTAC AACAGCAGGC CCAGCAACAG AAGCCCAGCG GACCATTCGG CTTGGACTCG
    CAGGTAATCA GCGAGATCGA AAAAGATTGG GTCCGAGTGG ACCAAATGGA GCAGAAGCCG
    CTACTGAACG TAGATATCTC ATCGCTGGAC GAGCCTATTA AGGTGAGGCC CACGCGGGCC
    GGCATTCACT ACCCGCAGGA GAAGTTCGAT CGGCTGATTT CTCGACAACA CTATATGCAG
    CAGACTTTAA CGACGTCCCC GTCATCCACG GCTGGAATGA CCAGTGGAGT CTCCCCGTAT
    CGCAGTCCTA TGCGCTTGCA GCACCAGCAG CCTCAACAGC AGCTGGAGAA CAATATACCA
    AATTTGGCGG ATGTTTTGCC AAAGCACGAT CCGCAGCTGG TAAAAGTTAT AAAAGGTGTA
    AGCAGCACGG ACACTCTAAA GGCGCGCGCG GCTATTAACG AGCTTGCCGC CATCATCGAG
    GCTCCAGAGA AGCAGGCTGT GCTGCGCGAC TATGAAGAAA TATTTATTCA GAATGTGCTT
    GCACAGTTTA AGAATCTCTC ACAAATACCA TCGGCTCAAT CCGTAGTTGT TTACCAGCCG
    CTATTATCCA TTCTGTATAC ATTTTTCCAT GCCAACATTC TGGGCAAAAC GCTGAGCGTG
    GCCTGCATCA AAAATCTAAT GTCGGCGCTG TTGAACCTTA TGGCTGATCC GAAGTTGGCC
    GTGGGTGATG ATAGTCAGTA TAACAAGGTT ATTAATGGCA TATGTCTAAA AGTGTTGGAC
    AAGGTAGATT TTACGAATTT AAACTGTGCT TTGATACGCC TATTACGGGA GACTTGTCCG
    GAGGCAAAGC TGCCGAAGTT TACAGACCTG CTGATGAAGT GCATATGGCG CAACGTTAAG
    ATGCTACCCG AACGAAGCAA CGAGCTGAAC TATGACGCCG TAATTCTCGA GGTGCACGAG
    TTCATGCTCG CGCTGCCTAG CACTTGGTGG CAAAACCGAC CGTCGGACAC TCCAATGCGC
    ACGATTAAGA CCATTCTGCA TAACATGGCA AAGGTGAAGG GGAATGCTAT TCTGCAGCAT
    CTCAACCAGA TTCCAACACA CTCGGAGCTA CATACTTATT TGATACGCAT TCTCAAGAAT
    TTCCAAAAGG ATGGCTCTGC TTCAGGAATT GGTGCCTCCC CCCAGAGGGC CAAGGAGATT
    GCCTCCAAGC GAATTTCGCA CCAAACACAT GATACAGTAT CACAGATCTT TAAGCTGATC
    TCAGATAGAG ATACCAAACA GCAAGGTCTG CAGAAACTTT ACGACTTTAA GCAACAAAAT
    CCTGATATCG ATCTAAGTAC CTTTCTGCAA GGCTCCAGTG CCCCATTTCA CAAGTACATC
    GAGGAGGGTT TAGCCGAGAT TGAGAGGAAT CAGAACGCTG GATCCACACA GGATAATCGG
    ACGGCAGCTA CTCGTTCTTA TCTCACAGAT GTCAACTACC AGAACAACGG TCCCGATCCC
    GATTTCTGGA TGGACCGCCT GCAGTATCAT ATGACCGGAG GGGCCGCCAA GCTAGCATCT
    GCACGTTCAG CAGACGATGG ATCCCATATG CTGGACAATA AGGTGGTTGA CGAGAACCTC
    TGCCTGAACG GAATGAACGC GCAAAAGGCG TCGCTCATTA AGCGAGAAAA GCGGGACATG
    TCACCAAATC GCTTGCAGCA TCTCCAGGCA AAGCTGGCTC AAATCAAGAA AGAGAATCAT
    GCCCAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001247129.1
    CDS447..6695
    Translation

    Target ORF information:

    RefSeq Version NM_001260200.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster mini spindles (msps), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001260200.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCCGAGG ACACAGAGTA CAAGAAGTTG CCGGTGGAGG AGCGGTGTGT CCATAAACTA 
    TGGAAAGCCC GCGTCGATGG TTACGAGGAG GCCGCAAAGA TCTTTCGCGA GCTGGATGAC
    GAGAAGTCGC CGGAATGGTC TAAATTTGCA GGGCTCATAA AGAAGATGGT AGTGGACTCT
    AATGCTCTGG CCCAGGAAAA GGGTCTAGAA GCGGCCCTCA TTTTTGTGGA GAACAGCGGG
    CTGGCGGGAC GCACAGTAGG CGACGTGATG ACAGGCATCG TTCAGAAGTG CATTGCGGCG
    CCAAAGACCA AAACCAAGGA GCTCTCCGTC CAAGTGGCTC TAATGTATGT GGAGATAGAG
    AAGCAGGAGG CCGTCGTCGA AGAGCTGGTT AAAGGTATGG AGGCCAAGAA CCCTAAGATT
    GTGTCTGCCT GCGTGGCAGC CACCACACTG GCCCTCCGCG AGTTCGGACA CAAGGTGATC
    GGCGTCAAGC CGTTAATTAA AAAGCTTGCT CCGCTAATGT CTGACCGAGA CAAGACGGTC
    CGAGATGAGG GCAAGCAGCT GGCTGTTGAG ATTTACCGTT GGATCGGAGC TGCAATGAAG
    GCACAGATCT CAACGCTACC ACAGGTAACG CTAAAGGAGC TGGAGGATGA ATTCGACAAA
    CTCAAAGGTG AACGCGTTGA ACCCTCCAGA TATCTAAAGT CCCAGCAGGA AAAGCAAGCG
    AAGATCGCCG ATGCTGCAGC GACCGAGGAT GCATATAATG AGGATGATGG CGAGGCTGGC
    GTTGAAGAAA TCGATCCTAT GGATCTTCTA GACCCCGTTG ACATTCTCTC CAAAATGCCC
    AAGGACTTCT ACGATAAGCT GGAGGAAAAG AAATGGACTC TGCGCAAAGA ATCGTTGGAA
    GTCCTGGAGA AACTGCTCAC TGACCACCCC AAGCTAGAGA ATGGCGAGTA TGGAGCGCTG
    GTAAGCGCTC TTAAAAAGGT TATAACCAAG GACTCCAACG TAGTCCTCGT TGCAATGGCC
    GGAAAATGTC TAGCCTTGCT AGCCAAAGGA TTGGCAAAGC GATTCTCAAA TTACGCATCC
    GCTTGTGTCC CATCCCTGTT GGAAAAATTT AAAGAGAAGA AACCTAATGT TGTGACAGCG
    CTGCGTGAAG CTATTGATGC CATATACGCC TCCACTTCTC TAGAGGCCCA GCAAGAGTCC
    ATTGTAGAGT CCCTGTCCAA CAAAAATCCA AGTGTTAAGT CCGAAACGGC CTTGTTTATT
    GCCCGTGCCC TAACCCGCAC CCAACCAACC GCGCTAAACA AAAAGCTGCT TAAACTTCTG
    ACCACCAGTC TAGTGAAAAC GCTTAACGAA CCAGATCCAA CCGTACGCGA CAGCAGCGCC
    GAAGCTCTTG GTACTTTAAT AAAGCTAATG GGAGACAAAG CGGTGACTCC TTTGCTCGCC
    GACGTCGATC CACTCAAAAT GGCCAAGATA AAGGAGTGCC AAGAAAAGGC GGAGATTAAA
    ATAAAGGTTG CAGGTCCAAA GAAGGAGACA CGACCTGCAT CTGCACCGAC TGCGAAAGCT
    GCTGCTCCGG CTAAGACAGT GGCTGGTAGT GTGGACCCTA AGCCAGTAAC CCGACCGGCC
    ACTACAGGGG CACGTAAGGT CTTGAAAAAA CCGGCGACGG TTAGTGGAGG AGGAGCTACA
    TCTGCCCCAA CTGCAGCTCT TAAAGCGGGT GGAAAACCCC TAGCCACGGA ACGCGAAATA
    ACCCCCGAGG AATTGCAAGA AAAATCCGAA GAAATTCTGC CAGCCGAAAT TCTTAATGGG
    CTGGTGGATT CCAACTGGAA GAACCGCCTT GCTGCTGTGG AACAGCTTCT GGGCGAAATA
    AGCGGTTTTG ATGCAAAACA GGCTGGCATA TCACAGATCC TGATCCGAAC AATCAGTGGA
    CGAAAGCCAG GCCTCAAAGA AATGAATTTC CAGGTACTCA AGTTTAAGCT AGACATAATC
    CGCAGTGTGG CTGAAAACTA TCCACTGACC ACGACAACCG TTGATCTGGT GATTAACGAA
    ATAATCGAGA AGCTAGCAGA CGCCAAGAAT GGTGCAGCAG CTGCAGATGT TTTGTCTGCC
    TTTGCCGAAG CCACTAAACT GGAGTATGTT GTGGGCAAAG TATTGAGCTT TGCTTTTGAA
    CAAAAATCGC CTAAAGTGCA ATCAGAGGCC TTTAACTGGG TTAACAGGTC AATCATAGAA
    TTCGGCTTCC AATTGCAGCC AAAGACACTT ATTGAAGATG TACGAAAAGG CGTGCAGAGC
    ACGAATCCAA CGGTTCGTGC TTCCGCCATT CAAATGGTAG GCACCATGTC TATGTATATG
    GGCAAAGCTC TAATGATGTT CTTCGACAGC GAGAAGCCTG CACTAAAGTC CCAGATCCAG
    GTAGAGTTTG ACAAGAATGT GGGCGAAAAA CCACCCAAGC CTGTTAGAGG AGTTCAACGT
    AGCAGCGGTG GAACCGCTGG TAATTCCCCA GACAATGAGG ACGATGATGG TGGGGCAGCC
    GGAGAAGAGG AGCCCATTAA TATGGCTGAT CTTCTACCAC GCGTTGATAT TGCTCCACAG
    ATTACGGAGG CATTGCTGAA GGAGATGTCC GACAAGGACT GGAAAACTCG CAACGAGGGT
    CTGACTAAGC TGCAGGCTAT TATTTCAGAG GCGCGGCTAA TCAAACCCAG CATTGGCGAC
    TTGGCTCCTG CTCTGGCCCA CCGTCTGGTA GATTCCAATG CTAAGATTGC CCAAACGACA
    CTTGCCATTT GCGAGCAGCT TGCCACAGCC ATGGGGGCTG GTTGTCGAAA TCACGTGCGG
    AACTTGTTCC CTGGCTTTTT ACACGCTCTG GGTGATAACA AGAGTTTTGT AAGAGCCGCT
    GCCCTCAATT GCATCAATAG TTTTGGCGAA AAGGGCGGCT ATAAGGAATT TTTTGAGAGC
    GAAATGATAG CCGATGCCCT GAAAGGAGGA TCCCCAGCTC TAAAGACAGA ATTGTGGGCT
    TGGCTGGCAG ATAAGCTGCC TGGTCTTCCC CCCAAGTCAG TATCGAAGGA GGATATCCAT
    TCAATGGTGC CGCACTTATA TGCTCACATC TGCGATCGAA ACGCCGATGT CCGGAAAAAT
    GCCAACGAGG CGGTGTTGGG CATTATGATT CACCTTGGAT TTGATGCAAT GAACCGCGCT
    CTGGACAAAC AGAAGCCCGC GTCCAAAAAG GATATACTTG CAGCACTCGA AAAAGCGCGT
    CCTAATCTTC CGGTAAAACC GCTGCCCAAA GGCAAGCATC AGGCTCCAAT ACCTGAGGAG
    CCGAAGCTAA AAACAGTGCG CGGCGGTGGA GCAGGTGGTG CGCCTGGAAT CCAGAAGAGT
    GCTACAGCCC GGGTTGCCGG AGGACAAGAC AAGCAGGTAC CAGCGCGCAA AAAGGACGAA
    GATATCGACA CATCGCCGCT ACTGTGCGCC AACAGTGCTA AAAACCAGCG GCTGCTAGAC
    GAGCAAAAAA TGAAGGTACT AAAGTGGACT TTTGTAACCC CAAGAGAGGA ATTCACCGAA
    CTGTTACGCG ACCAAATGAT GACCGCAAAC GTAAATAAAG CACTGATAGC CAACATGTTC
    CACGACGATT TTCGCTACCA CTTGAAAGTC ATCGAGCAGT TGAGCGAAGA TCTGGCTGGT
    AACAGTAAGG CGCTGGTGTG TAATCTGGAC CTAATACTTA AATGGCTGAC GCTGCGATTT
    TACGATACGA ACCCTTCTGT GTTGATTAAG GGTCTCGAAT ATCTAGTACA GGTTTTCCAG
    GTACTCATTG ACGAGGAATA CATTCTGGCC GAGAACGAGG GAAGCAGTTT TGTCCCACAT
    CTGCTTTTAA AGATCGGAGA TCCAAAGGAT GCTGTTCGAA ATGGCGTACG CCGCGTTCTG
    CGGCAAGTAA TATTGGTCTT CCCTTTCGTC AAGGTCTTTG GCTATGTGAT GGAGGGTCTA
    AAATCGAAGA ATGCCCGTCA GCGTACTGAG TGCCTTGACG AGTTAACCTT CCTAATTGAA
    AGCTATGGCA TGAACATCTG TCCCCAATCG GCGGTGCGCG AGATTGCTCG CCAGATTTCC
    GACCGGGATA ATTCCGTACG CAACGCAGCA CTCAACTGCA TCGTCCAGGT CTTCTTTTTG
    TCCGGGGAAA AGACTTACAA GATGATTGGC CATCTTAACG AAAAGGACCT CTCAATGCTA
    GATGAGCGCA TCAAGCGAGC CAAAAAGACT AAGAAGCCCA CGCCACCGCC ATCTGTTGAT
    GTGCCGGCAC CTCAACGACA TGACAGCATC GAAATCGAAG ATGCTGAAGT GGGCAACGGT
    TGCGACGAGC TTCCACCACC AGATGAGGAC GGTTTGCAGC GAGAACTAAA TGCGCGTGGG
    CAGAACAACA TGACAGATCG GAGCGCCACA AACGTGGTTG CCTACTTGAG CTCCCTGACT
    CGACAGGCAA CTTTCGACCA GGCACCTTCG TCACAGCTGC TTCTACTCCA GCAACAGCTT
    CAACAGTTAC AACAGCAGGC CCAGCAACAG AAGCCCAGCG GACCATTCGG CTTGGACTCG
    CAGGTAATCA GCGAGATCGA AAAAGATTGG GTCCGAGTGG ACCAAATGGA GCAGAAGCCG
    CTACTGAACG TAGATATCTC ATCGCTGGAC GAGCCTATTA AGGTGAGGCC CACGCGGGCC
    GGCATTCACT ACCCGCAGGA GAAGTTCGAT CGGCTGATTT CTCGACAACA CTATATGCAG
    CAGACTTTAA CGACGTCCCC GTCATCCACG GCTGGAATGA CCAGTGGAGT CTCCCCGTAT
    CGCAGTCCTA TGCGCTTGCA GCACCAGCAG CCTCAACAGC AGCTGGAGAA CAATATACCA
    AATTTGGCGG ATGTTTTGCC AAAGCACGAT CCGCAGCTGG TAAAAGTTAT AAAAGGTGTA
    AGCAGCACGG ACACTCTAAA GGCGCGCGCG GCTATTAACG AGCTTGCCGC CATCATCGAG
    GCTCCAGAGA AGCAGGCTGT GCTGCGCGAC TATGAAGAAA TATTTATTCA GAATGTGCTT
    GCACAGTTTA AGAATCTCTC ACAAATACCA TCGGCTCAAT CCGTAGTTGT TTACCAGCCG
    CTATTATCCA TTCTGTATAC ATTTTTCCAT GCCAACATTC TGGGCAAAAC GCTGAGCGTG
    GCCTGCATCA AAAATCTAAT GTCGGCGCTG TTGAACCTTA TGGCTGATCC GAAGTTGGCC
    GTGGGTGATG ATAGTCAGTA TAACAAGGTT ATTAATGGCA TATGTCTAAA AGTGTTGGAC
    AAGGTAGATT TTACGAATTT AAACTGTGCT TTGATACGCC TATTACGGGA GACTTGTCCG
    GAGGCAAAGC TGCCGAAGTT TACAGACCTG CTGATGAAGT GCATATGGCG CAACGTTAAG
    ATGCTACCCG AACGAAGCAA CGAGCTGAAC TATGACGCCG TAATTCTCGA GGTGCACGAG
    TTCATGCTCG CGCTGCCTAG CACTTGGTGG CAAAACCGAC CGTCGGACAC TCCAATGCGC
    ACGATTAAGA CCATTCTGCA TAACATGGCA AAGGTGAAGG GGAATGCTAT TCTGCAGCAT
    CTCAACCAGA TTCCAACACA CTCGGAGCTA CATACTTATT TGATACGCAT TCTCAAGAAT
    TTCCAAAAGG ATGGCTCTGC TTCAGGAATT GGTGCCTCCC CCCAGAGGGC CAAGGAGATT
    GCCTCCAAGC GAATTTCGCA CCAAACACAT GATACAGTAT CACAGATCTT TAAGCTGATC
    TCAGATAGAG ATACCAAACA GCAAGGTCTG CAGAAACTTT ACGACTTTAA GCAACAAAAT
    CCTGATATCG ATCTAAGTAC CTTTCTGCAA GGCTCCAGTG CCCCATTTCA CAAGTACATC
    GAGGAGGGTT TAGCCGAGAT TGAGAGGAAT CAGAACGCTG GATCCACACA GGATAATCGG
    ACGGCAGCTA CTCGTTCTTA TCTCACAGAT GTCAACTACC AGAACAACGG TCCCGATCCC
    GATTTCTGGA TGGACCGCCT GCAGTATCAT ATGACCGGAG GGGCCGCCAA GCTAGCATCT
    GCACGTTCAG CAGACGATGG ATCCCATATG CTGGACAATA AGGTGGTTGA CGAGAACCTC
    TGCCTGAACG GAATGAACGC GCAAAAGGCG TCGCTCATTA AGCGAGAAAA GCGGGACATG
    TCACCAAATC GCTTGCAGCA TCTCCAGGCA AAGCTGGCTC AAATCAAGAA AGAGAATCAT
    GCCCAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08420
    Clone ID Related Accession (Same CDS sequence) NM_001260201.2
    Accession Version NM_001260201.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6225bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product mini spindles, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001260201.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCCGAGG ACACAGAGTA CAAGAAGTTG CCGGTGGAGG AGCGGTGTGT CCATAAACTA 
    TGGAAAGCCC GCGTCGATGG TTACGAGGAG GCCGCAAAGA TCTTTCGCGA GCTGGATGAC
    GAGAAGTCGC CGGAATGGTC TAAATTTGCA GGGCTCATAA AGAAGATGGT AGTGGACTCT
    AATGCTCTGG CCCAGGAAAA GGGTCTAGAA GCGGCCCTCA TTTTTGTGGA GAACAGCGGG
    CTGGCGGGAC GCACAGTAGG CGACGTGATG ACAGGCATCG TTCAGAAGTG CATTGCGGCG
    CCAAAGACCA AAACCAAGGA GCTCTCCGTC CAAGTGGCTC TAATGTATGT GGAGATAGAG
    AAGCAGGAGG CCGTCGTCGA AGAGCTGGTT AAAGGTATGG AGGCCAAGAA CCCTAAGATT
    GTGTCTGCCT GCGTGGCAGC CACCACACTG GCCCTCCGCG AGTTCGGACA CAAGGTGATC
    GGCGTCAAGC CGTTAATTAA AAAGCTTGCT CCGCTAATGT CTGACCGAGA CAAGACGGTC
    CGAGATGAGG GCAAGCAGCT GGCTGTTGAG ATTTACCGTT GGATCGGAGC TGCAATGAAG
    GCACAGATCT CAACGCTACC ACAGGTAACG CTAAAGGAGC TGGAGGATGA ATTCGACAAA
    CTCAAAGGTG AACGCGTTGA ACCCTCCAGA TATCTAAAGT CCCAGCAGGA AAAGCAAGCG
    AAGATCGCCG ATGCTGCAGC GACCGAGGAT GCATATAATG AGGATGATGG CGAGGCTGGC
    GTTGAAGAAA TCGATCCTAT GGATCTTCTA GACCCCGTTG ACATTCTCTC CAAAATGCCC
    AAGGACTTCT ACGATAAGCT GGAGGAAAAG AAATGGACTC TGCGCAAAGA ATCGTTGGAA
    GTCCTGGAGA AACTGCTCAC TGACCACCCC AAGCTAGAGA ATGGCGAGTA TGGAGCGCTG
    GTAAGCGCTC TTAAAAAGGT TATAACCAAG GACTCCAACG TAGTCCTCGT TGCAATGGCC
    GGAAAATGTC TAGCCTTGCT AGCCAAAGGA TTGGCAAAGC GATTCTCAAA TTACGCATCC
    GCTTGTGTCC CATCCCTGTT GGAAAAATTT AAAGAGAAGA AACCTAATGT TGTGACAGCG
    CTGCGTGAAG CTATTGATGC CATATACGCC TCCACTTCTC TAGAGGCCCA GCAAGAGTCC
    ATTGTAGAGT CCCTGTCCAA CAAAAATCCA AGTGTTAAGT CCGAAACGGC CTTGTTTATT
    GCCCGTGCCC TAACCCGCAC CCAACCAACC GCGCTAAACA AAAAGCTGCT TAAACTTCTG
    ACCACCAGTC TAGTGAAAAC GCTTAACGAA CCAGATCCAA CCGTACGCGA CAGCAGCGCC
    GAAGCTCTTG GTACTTTAAT AAAGCTAATG GGAGACAAAG CGGTGACTCC TTTGCTCGCC
    GACGTCGATC CACTCAAAAT GGCCAAGATA AAGGAGTGCC AAGAAAAGGC GGAGATTAAA
    ATAAAGGTTG CAGGTCCAAA GAAGGAGACA CGACCTGCAT CTGCACCGAC TGCGAAAGCT
    GCTGCTCCGG CTAAGACAGT GGCTGGTAGT GTGGACCCTA AGCCAGTAAC CCGACCGGCC
    ACTACAGGGG CACGTAAGGT CTTGAAAAAA CCGGCGACGG TTAGTGGAGG AGGAGCTACA
    TCTGCCCCAA CTGCAGCTCT TAAAGCGGGT GGAAAACCCC TAGCCACGGA ACGCGAAATA
    ACCCCCGAGG AATTGCAAGA AAAATCCGAA GAAATTCTGC CAGCCGAAAT TCTTAATGGG
    CTGGTGGATT CCAACTGGAA GAACCGCCTT GCTGCTGTGG AACAGCTTCT GGGCGAAATA
    AGCGGTTTTG ATGCAAAACA GGCTGGCATA TCACAGATCC TGATCCGAAC AATCAGTGGA
    CGAAAGCCAG GCCTCAAAGA AATGAATTTC CAGGTACTCA AGTTTAAGCT AGACATAATC
    CGCAGTGTGG CTGAAAACTA TCCACTGACC ACGACAACCG TTGATCTGGT GATTAACGAA
    ATAATCGAGA AGCTAGCAGA CGCCAAGAAT GGTGCAGCAG CTGCAGATGT TTTGTCTGCC
    TTTGCCGAAG CCACTAAACT GGAGTATGTT GTGGGCAAAG TATTGAGCTT TGCTTTTGAA
    CAAAAATCGC CTAAAGTGCA ATCAGAGGCC TTTAACTGGG TTAACAGGTC AATCATAGAA
    TTCGGCTTCC AATTGCAGCC AAAGACACTT ATTGAAGATG TACGAAAAGG CGTGCAGAGC
    ACGAATCCAA CGGTTCGTGC TTCCGCCATT CAAATGGTAG GCACCATGTC TATGTATATG
    GGCAAAGCTC TAATGATGTT CTTCGACAGC GAGAAGCCTG CACTAAAGTC CCAGATCCAG
    GTAGAGTTTG ACAAGAATGT GGGCGAAAAA CCACCCAAGC CTGTTAGAGG AGTTCAACGT
    AGCAGCGGTG GAACCGCTGG TAATTCCCCA GACAATGAGG ACGATGATGG TGGGGCAGCC
    GGAGAAGAGG AGCCCATTAA TATGGCTGAT CTTCTACCAC GCGTTGATAT TGCTCCACAG
    ATTACGGAGG CATTGCTGAA GGAGATGTCC GACAAGGACT GGAAAACTCG CAACGAGGGT
    CTGACTAAGC TGCAGGCTAT TATTTCAGAG GCGCGGCTAA TCAAACCCAG CATTGGCGAC
    TTGGCTCCTG CTCTGGCCCA CCGTCTGGTA GATTCCAATG CTAAGATTGC CCAAACGACA
    CTTGCCATTT GCGAGCAGCT TGCCACAGCC ATGGGGGCTG GTTGTCGAAA TCACGTGCGG
    AACTTGTTCC CTGGCTTTTT ACACGCTCTG GGTGATAACA AGAGTTTTGT AAGAGCCGCT
    GCCCTCAATT GCATCAATAG TTTTGGCGAA AAGGGCGGCT ATAAGGAATT TTTTGAGAGC
    GAAATGATAG CCGATGCCCT GAAAGGAGGA TCCCCAGCTC TAAAGACAGA ATTGTGGGCT
    TGGCTGGCAG ATAAGCTGCC TGGTCTTCCC CCCAAGTCAG TATCGAAGGA GGATATCCAT
    TCAATGGTGC CGCACTTATA TGCTCACATC TGCGATCGAA ACGCCGATGT CCGGAAAAAT
    GCCAACGAGG CGGTGTTGGG CATTATGATT CACCTTGGAT TTGATGCAAT GAACCGCGCT
    CTGGACAAAC AGAAGCCCGC GTCCAAAAAG GATATACTTG CAGCACTCGA AAAAGCGCGT
    CCTAATCTTC CGGTAAAACC GCTGCCCAAA GGCAAGCATC AGGCTCCAAT ACCTGAGGAG
    CCGAAGCTAA AAACAGTGCG CGGCGGTGGA GCAGGTGGTG CGCCTGGAAT CCAGAAGAGT
    GCTACAGCCC GGGTTGCCGG AGGACAAGAC AAGCAGGTAC CAGCGCGCAA AAAGGACGAA
    GATATCGACA CATCGCCGCT ACTGTGCGCC AACAGTGCTA AAAACCAGCG GCTGCTAGAC
    GAGCAAAAAA TGAAGGTACT AAAGTGGACT TTTGTAACCC CAAGAGAGGA ATTCACCGAA
    CTGTTACGCG ACCAAATGAT GACCGCAAAC GTAAATAAAG CACTGATAGC CAACATGTTC
    CACGACGATT TTCGCTACCA CTTGAAAGTC ATCGAGCAGT TGAGCGAAGA TCTGGCTGGT
    AACAGTAAGG CGCTGGTGTG TAATCTGGAC CTAATACTTA AATGGCTGAC GCTGCGATTT
    TACGATACGA ACCCTTCTGT GTTGATTAAG GGTCTCGAAT ATCTAGTACA GGTTTTCCAG
    GTACTCATTG ACGAGGAATA CATTCTGGCC GAGAACGAGG GAAGCAGTTT TGTCCCACAT
    CTGCTTTTAA AGATCGGAGA TCCAAAGGAT GCTGTTCGAA ATGGCGTACG CCGCGTTCTG
    CGGCAAGTAA TATTGGTCTT CCCTTTCGTC AAGGTCTTTG GCTATGTGAT GGAGGGTCTA
    AAATCGAAGA ATGCCCGTCA GCGTACTGAG TGCCTTGACG AGTTAACCTT CCTAATTGAA
    AGCTATGGCA TGAACATCTG TCCCCAATCG GCGGTGCGCG AGATTGCTCG CCAGATTTCC
    GACCGGGATA ATTCCGTACG CAACGCAGCA CTCAACTGCA TCGTCCAGGT CTTCTTTTTG
    TCCGGGGAAA AGACTTACAA GATGATTGGC CATCTTAACG AAAAGGACCT CTCAATGCTA
    GATGAGCGCA TCAAGCGAGC CAAAAAGACT AAGAAGCCCA CGCCACCGCC ATCTGTTGAT
    GTGCCGGCAC CTCAACGACA TGACAGCATC GAAATCGAAG ATGCTGAAGT GGGCAACGGT
    TGCGACGAGC TTCCACCACC AGATGAGGAC GGTTTGCAGC GAGAACTAAA TGCGCGTGGG
    CAGAACAACA TGACAGATCG GAGCGCCACA AACGTGGTTG CCTACTTGAG CTCCCTGACT
    CGACAGGCAA CTTTCGACCA GGCACCTTCG TCACAGCTGC TTCTACTCCA GCAACAGCTT
    CAACAGTTAC AACAGCAGGC CCAGCAACAG AAGCCCAGCG GACCATTCGG CTTGGACTCG
    CAGGTAATCA GCGAGATCGA AAAAGATTGG GTCCGAGTGG ACCAAATGGA GCAGAAGCCG
    CTACTGAACG TAGATATCTC ATCGCTGGAC GAGCCTATTA AGGTGAGGCC CACGCGGGCC
    GGCATTCACT ACCCGCAGGA GAAGTTCGAT CGGCTGATTT CTCGACAACA CTATATGCAG
    CAGACTTTAA CGACGTCCCC GTCATCCACG GCTGGAATGA CCAGTGGAGT CTCCCCGTAT
    CGCAGTCCTA TGCGCTTGCA GCACCAGCAG CCTCAACAGC AGCTGGAGAA CAATATACCA
    AATTTGGCGG ATGTTTTGCC AAAGCACGAT CCGCAGCTGG TAAAAGTTAT AAAAGGTGTA
    AGCAGCACGG ACACTCTAAA GGCGCGCGCG GCTATTAACG AGCTTGCCGC CATCATCGAG
    GCTCCAGAGA AGCAGGCTGT GCTGCGCGAC TATGAAGAAA TATTTATTCA GAATGTGCTT
    GCACAGTTTA AGAATCTCTC ACAAATACCA TCGGCTCAAT CCGTAGTTGT TTACCAGCCG
    CTATTATCCA TTCTGTATAC ATTTTTCCAT GCCAACATTC TGGGCAAAAC GCTGAGCGTG
    GCCTGCATCA AAAATCTAAT GTCGGCGCTG TTGAACCTTA TGGCTGATCC GAAGTTGGCC
    GTGGGTGATG ATAGTCAGTA TAACAAGGTT ATTAATGGCA TATGTCTAAA AGTGTTGGAC
    AAGGTAGATT TTACGAATTT AAACTGTGCT TTGATACGCC TATTACGGGA GACTTGTCCG
    GAGGCAAAGC TGCCGAAGTT TACAGACCTG CTGATGAAGT GCATATGGCG CAACGTTAAG
    ATGCTACCCG AACGAAGCAA CGAGCTGAAC TATGACGCCG TAATTCTCGA GGTGCACGAG
    TTCATGCTCG CGCTGCCTAG CACTTGGTGG CAAAACCGAC CGTCGGACAC TCCAATGCGC
    ACGATTAAGA CCATTCTGCA TAACATGGCA AAGGTGAAGG GGAATGCTAT TCTGCAGCAT
    CTCAACCAGA TTCCAACACA CTCGGAGCTA CATACTTATT TGATACGCAT TCTCAAGAAT
    TTCCAAAAGG ATGGCTCTGC TTCAGGAATT GGTGCCTCCC CCCAGAGGGC CAAGGAGATT
    GCCTCCAAGC GAATTTCGCA CCAAACACAT GATACAGTAT CACAGATCTT TAAGCTGATC
    TCAGATAGAG ATACCAAACA GCAAGGTCTG CAGAAACTTT ACGACTTTAA GCAACAAAAT
    CCTGATATCG ATCTAAGTAC CTTTCTGCAA GGCTCCAGTG CCCCATTTCA CAAGTACATC
    GAGGAGGGTT TAGCCGAGAT TGAGAGGAAT CAGAACGCTG GATCCACACA GGATAATCGG
    ACGGATGTCA ACTACCAGAA CAACGGTCCC GATCCCGATT TCTGGATGGA CCGCCTGCAG
    TATCATATGA CCGGAGGGGC CGCCAAGCTA GCATCTGCAC GTTCAGCAGA CGATGGATCC
    CATATGCTGG ACAATAAGGT GGTTGACGAG AACCTCTGCC TGAACGGAAT GAACGCGCAA
    AAGGCGTCGC TCATTAAGCG AGAAAAGCGG GACATGTCAC CAAATCGCTT GCAGCATCTC
    CAGGCAAAGC TGGCTCAAAT CAAGAAAGAG AATCATGCCC AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001247130.1
    CDS447..6671
    Translation

    Target ORF information:

    RefSeq Version NM_001260201.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster mini spindles (msps), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001260201.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGGCCGAGG ACACAGAGTA CAAGAAGTTG CCGGTGGAGG AGCGGTGTGT CCATAAACTA 
    TGGAAAGCCC GCGTCGATGG TTACGAGGAG GCCGCAAAGA TCTTTCGCGA GCTGGATGAC
    GAGAAGTCGC CGGAATGGTC TAAATTTGCA GGGCTCATAA AGAAGATGGT AGTGGACTCT
    AATGCTCTGG CCCAGGAAAA GGGTCTAGAA GCGGCCCTCA TTTTTGTGGA GAACAGCGGG
    CTGGCGGGAC GCACAGTAGG CGACGTGATG ACAGGCATCG TTCAGAAGTG CATTGCGGCG
    CCAAAGACCA AAACCAAGGA GCTCTCCGTC CAAGTGGCTC TAATGTATGT GGAGATAGAG
    AAGCAGGAGG CCGTCGTCGA AGAGCTGGTT AAAGGTATGG AGGCCAAGAA CCCTAAGATT
    GTGTCTGCCT GCGTGGCAGC CACCACACTG GCCCTCCGCG AGTTCGGACA CAAGGTGATC
    GGCGTCAAGC CGTTAATTAA AAAGCTTGCT CCGCTAATGT CTGACCGAGA CAAGACGGTC
    CGAGATGAGG GCAAGCAGCT GGCTGTTGAG ATTTACCGTT GGATCGGAGC TGCAATGAAG
    GCACAGATCT CAACGCTACC ACAGGTAACG CTAAAGGAGC TGGAGGATGA ATTCGACAAA
    CTCAAAGGTG AACGCGTTGA ACCCTCCAGA TATCTAAAGT CCCAGCAGGA AAAGCAAGCG
    AAGATCGCCG ATGCTGCAGC GACCGAGGAT GCATATAATG AGGATGATGG CGAGGCTGGC
    GTTGAAGAAA TCGATCCTAT GGATCTTCTA GACCCCGTTG ACATTCTCTC CAAAATGCCC
    AAGGACTTCT ACGATAAGCT GGAGGAAAAG AAATGGACTC TGCGCAAAGA ATCGTTGGAA
    GTCCTGGAGA AACTGCTCAC TGACCACCCC AAGCTAGAGA ATGGCGAGTA TGGAGCGCTG
    GTAAGCGCTC TTAAAAAGGT TATAACCAAG GACTCCAACG TAGTCCTCGT TGCAATGGCC
    GGAAAATGTC TAGCCTTGCT AGCCAAAGGA TTGGCAAAGC GATTCTCAAA TTACGCATCC
    GCTTGTGTCC CATCCCTGTT GGAAAAATTT AAAGAGAAGA AACCTAATGT TGTGACAGCG
    CTGCGTGAAG CTATTGATGC CATATACGCC TCCACTTCTC TAGAGGCCCA GCAAGAGTCC
    ATTGTAGAGT CCCTGTCCAA CAAAAATCCA AGTGTTAAGT CCGAAACGGC CTTGTTTATT
    GCCCGTGCCC TAACCCGCAC CCAACCAACC GCGCTAAACA AAAAGCTGCT TAAACTTCTG
    ACCACCAGTC TAGTGAAAAC GCTTAACGAA CCAGATCCAA CCGTACGCGA CAGCAGCGCC
    GAAGCTCTTG GTACTTTAAT AAAGCTAATG GGAGACAAAG CGGTGACTCC TTTGCTCGCC
    GACGTCGATC CACTCAAAAT GGCCAAGATA AAGGAGTGCC AAGAAAAGGC GGAGATTAAA
    ATAAAGGTTG CAGGTCCAAA GAAGGAGACA CGACCTGCAT CTGCACCGAC TGCGAAAGCT
    GCTGCTCCGG CTAAGACAGT GGCTGGTAGT GTGGACCCTA AGCCAGTAAC CCGACCGGCC
    ACTACAGGGG CACGTAAGGT CTTGAAAAAA CCGGCGACGG TTAGTGGAGG AGGAGCTACA
    TCTGCCCCAA CTGCAGCTCT TAAAGCGGGT GGAAAACCCC TAGCCACGGA ACGCGAAATA
    ACCCCCGAGG AATTGCAAGA AAAATCCGAA GAAATTCTGC CAGCCGAAAT TCTTAATGGG
    CTGGTGGATT CCAACTGGAA GAACCGCCTT GCTGCTGTGG AACAGCTTCT GGGCGAAATA
    AGCGGTTTTG ATGCAAAACA GGCTGGCATA TCACAGATCC TGATCCGAAC AATCAGTGGA
    CGAAAGCCAG GCCTCAAAGA AATGAATTTC CAGGTACTCA AGTTTAAGCT AGACATAATC
    CGCAGTGTGG CTGAAAACTA TCCACTGACC ACGACAACCG TTGATCTGGT GATTAACGAA
    ATAATCGAGA AGCTAGCAGA CGCCAAGAAT GGTGCAGCAG CTGCAGATGT TTTGTCTGCC
    TTTGCCGAAG CCACTAAACT GGAGTATGTT GTGGGCAAAG TATTGAGCTT TGCTTTTGAA
    CAAAAATCGC CTAAAGTGCA ATCAGAGGCC TTTAACTGGG TTAACAGGTC AATCATAGAA
    TTCGGCTTCC AATTGCAGCC AAAGACACTT ATTGAAGATG TACGAAAAGG CGTGCAGAGC
    ACGAATCCAA CGGTTCGTGC TTCCGCCATT CAAATGGTAG GCACCATGTC TATGTATATG
    GGCAAAGCTC TAATGATGTT CTTCGACAGC GAGAAGCCTG CACTAAAGTC CCAGATCCAG
    GTAGAGTTTG ACAAGAATGT GGGCGAAAAA CCACCCAAGC CTGTTAGAGG AGTTCAACGT
    AGCAGCGGTG GAACCGCTGG TAATTCCCCA GACAATGAGG ACGATGATGG TGGGGCAGCC
    GGAGAAGAGG AGCCCATTAA TATGGCTGAT CTTCTACCAC GCGTTGATAT TGCTCCACAG
    ATTACGGAGG CATTGCTGAA GGAGATGTCC GACAAGGACT GGAAAACTCG CAACGAGGGT
    CTGACTAAGC TGCAGGCTAT TATTTCAGAG GCGCGGCTAA TCAAACCCAG CATTGGCGAC
    TTGGCTCCTG CTCTGGCCCA CCGTCTGGTA GATTCCAATG CTAAGATTGC CCAAACGACA
    CTTGCCATTT GCGAGCAGCT TGCCACAGCC ATGGGGGCTG GTTGTCGAAA TCACGTGCGG
    AACTTGTTCC CTGGCTTTTT ACACGCTCTG GGTGATAACA AGAGTTTTGT AAGAGCCGCT
    GCCCTCAATT GCATCAATAG TTTTGGCGAA AAGGGCGGCT ATAAGGAATT TTTTGAGAGC
    GAAATGATAG CCGATGCCCT GAAAGGAGGA TCCCCAGCTC TAAAGACAGA ATTGTGGGCT
    TGGCTGGCAG ATAAGCTGCC TGGTCTTCCC CCCAAGTCAG TATCGAAGGA GGATATCCAT
    TCAATGGTGC CGCACTTATA TGCTCACATC TGCGATCGAA ACGCCGATGT CCGGAAAAAT
    GCCAACGAGG CGGTGTTGGG CATTATGATT CACCTTGGAT TTGATGCAAT GAACCGCGCT
    CTGGACAAAC AGAAGCCCGC GTCCAAAAAG GATATACTTG CAGCACTCGA AAAAGCGCGT
    CCTAATCTTC CGGTAAAACC GCTGCCCAAA GGCAAGCATC AGGCTCCAAT ACCTGAGGAG
    CCGAAGCTAA AAACAGTGCG CGGCGGTGGA GCAGGTGGTG CGCCTGGAAT CCAGAAGAGT
    GCTACAGCCC GGGTTGCCGG AGGACAAGAC AAGCAGGTAC CAGCGCGCAA AAAGGACGAA
    GATATCGACA CATCGCCGCT ACTGTGCGCC AACAGTGCTA AAAACCAGCG GCTGCTAGAC
    GAGCAAAAAA TGAAGGTACT AAAGTGGACT TTTGTAACCC CAAGAGAGGA ATTCACCGAA
    CTGTTACGCG ACCAAATGAT GACCGCAAAC GTAAATAAAG CACTGATAGC CAACATGTTC
    CACGACGATT TTCGCTACCA CTTGAAAGTC ATCGAGCAGT TGAGCGAAGA TCTGGCTGGT
    AACAGTAAGG CGCTGGTGTG TAATCTGGAC CTAATACTTA AATGGCTGAC GCTGCGATTT
    TACGATACGA ACCCTTCTGT GTTGATTAAG GGTCTCGAAT ATCTAGTACA GGTTTTCCAG
    GTACTCATTG ACGAGGAATA CATTCTGGCC GAGAACGAGG GAAGCAGTTT TGTCCCACAT
    CTGCTTTTAA AGATCGGAGA TCCAAAGGAT GCTGTTCGAA ATGGCGTACG CCGCGTTCTG
    CGGCAAGTAA TATTGGTCTT CCCTTTCGTC AAGGTCTTTG GCTATGTGAT GGAGGGTCTA
    AAATCGAAGA ATGCCCGTCA GCGTACTGAG TGCCTTGACG AGTTAACCTT CCTAATTGAA
    AGCTATGGCA TGAACATCTG TCCCCAATCG GCGGTGCGCG AGATTGCTCG CCAGATTTCC
    GACCGGGATA ATTCCGTACG CAACGCAGCA CTCAACTGCA TCGTCCAGGT CTTCTTTTTG
    TCCGGGGAAA AGACTTACAA GATGATTGGC CATCTTAACG AAAAGGACCT CTCAATGCTA
    GATGAGCGCA TCAAGCGAGC CAAAAAGACT AAGAAGCCCA CGCCACCGCC ATCTGTTGAT
    GTGCCGGCAC CTCAACGACA TGACAGCATC GAAATCGAAG ATGCTGAAGT GGGCAACGGT
    TGCGACGAGC TTCCACCACC AGATGAGGAC GGTTTGCAGC GAGAACTAAA TGCGCGTGGG
    CAGAACAACA TGACAGATCG GAGCGCCACA AACGTGGTTG CCTACTTGAG CTCCCTGACT
    CGACAGGCAA CTTTCGACCA GGCACCTTCG TCACAGCTGC TTCTACTCCA GCAACAGCTT
    CAACAGTTAC AACAGCAGGC CCAGCAACAG AAGCCCAGCG GACCATTCGG CTTGGACTCG
    CAGGTAATCA GCGAGATCGA AAAAGATTGG GTCCGAGTGG ACCAAATGGA GCAGAAGCCG
    CTACTGAACG TAGATATCTC ATCGCTGGAC GAGCCTATTA AGGTGAGGCC CACGCGGGCC
    GGCATTCACT ACCCGCAGGA GAAGTTCGAT CGGCTGATTT CTCGACAACA CTATATGCAG
    CAGACTTTAA CGACGTCCCC GTCATCCACG GCTGGAATGA CCAGTGGAGT CTCCCCGTAT
    CGCAGTCCTA TGCGCTTGCA GCACCAGCAG CCTCAACAGC AGCTGGAGAA CAATATACCA
    AATTTGGCGG ATGTTTTGCC AAAGCACGAT CCGCAGCTGG TAAAAGTTAT AAAAGGTGTA
    AGCAGCACGG ACACTCTAAA GGCGCGCGCG GCTATTAACG AGCTTGCCGC CATCATCGAG
    GCTCCAGAGA AGCAGGCTGT GCTGCGCGAC TATGAAGAAA TATTTATTCA GAATGTGCTT
    GCACAGTTTA AGAATCTCTC ACAAATACCA TCGGCTCAAT CCGTAGTTGT TTACCAGCCG
    CTATTATCCA TTCTGTATAC ATTTTTCCAT GCCAACATTC TGGGCAAAAC GCTGAGCGTG
    GCCTGCATCA AAAATCTAAT GTCGGCGCTG TTGAACCTTA TGGCTGATCC GAAGTTGGCC
    GTGGGTGATG ATAGTCAGTA TAACAAGGTT ATTAATGGCA TATGTCTAAA AGTGTTGGAC
    AAGGTAGATT TTACGAATTT AAACTGTGCT TTGATACGCC TATTACGGGA GACTTGTCCG
    GAGGCAAAGC TGCCGAAGTT TACAGACCTG CTGATGAAGT GCATATGGCG CAACGTTAAG
    ATGCTACCCG AACGAAGCAA CGAGCTGAAC TATGACGCCG TAATTCTCGA GGTGCACGAG
    TTCATGCTCG CGCTGCCTAG CACTTGGTGG CAAAACCGAC CGTCGGACAC TCCAATGCGC
    ACGATTAAGA CCATTCTGCA TAACATGGCA AAGGTGAAGG GGAATGCTAT TCTGCAGCAT
    CTCAACCAGA TTCCAACACA CTCGGAGCTA CATACTTATT TGATACGCAT TCTCAAGAAT
    TTCCAAAAGG ATGGCTCTGC TTCAGGAATT GGTGCCTCCC CCCAGAGGGC CAAGGAGATT
    GCCTCCAAGC GAATTTCGCA CCAAACACAT GATACAGTAT CACAGATCTT TAAGCTGATC
    TCAGATAGAG ATACCAAACA GCAAGGTCTG CAGAAACTTT ACGACTTTAA GCAACAAAAT
    CCTGATATCG ATCTAAGTAC CTTTCTGCAA GGCTCCAGTG CCCCATTTCA CAAGTACATC
    GAGGAGGGTT TAGCCGAGAT TGAGAGGAAT CAGAACGCTG GATCCACACA GGATAATCGG
    ACGGATGTCA ACTACCAGAA CAACGGTCCC GATCCCGATT TCTGGATGGA CCGCCTGCAG
    TATCATATGA CCGGAGGGGC CGCCAAGCTA GCATCTGCAC GTTCAGCAGA CGATGGATCC
    CATATGCTGG ACAATAAGGT GGTTGACGAG AACCTCTGCC TGAACGGAAT GAACGCGCAA
    AAGGCGTCGC TCATTAAGCG AGAAAAGCGG GACATGTCAC CAAATCGCTT GCAGCATCTC
    CAGGCAAAGC TGGCTCAAAT CAAGAAAGAG AATCATGCCC AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08421
    Clone ID Related Accession (Same CDS sequence) NM_001260202.1 , NM_001170156.2
    Accession Version NM_001260202.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6153bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product mini spindles, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCGAGG ACACAGAGTA CAAGAAGTTG CCGGTGGAGG AGCGGTGTGT CCATAAACTA 
    TGGAAAGCCC GCGTCGATGG TTACGAGGAG GCCGCAAAGA TCTTTCGCGA GCTGGATGAC
    GAGAAGTCGC CGGAATGGTC TAAATTTGCA GGGCTCATAA AGAAGATGGT AGTGGACTCT
    AATGCTCTGG CCCAGGAAAA GGGTCTAGAA GCGGCCCTCA TTTTTGTGGA GAACAGCGGG
    CTGGCGGGAC GCACAGTAGG CGACGTGATG ACAGGCATCG TTCAGAAGTG CATTGCGGCG
    CCAAAGACCA AAACCAAGGA GCTCTCCGTC CAAGTGGCTC TAATGTATGT GGAGATAGAG
    AAGCAGGAGG CCGTCGTCGA AGAGCTGGTT AAAGGTATGG AGGCCAAGAA CCCTAAGATT
    GTGTCTGCCT GCGTGGCAGC CACCACACTG GCCCTCCGCG AGTTCGGACA CAAGGTGATC
    GGCGTCAAGC CGTTAATTAA AAAGCTTGCT CCGCTAATGT CTGACCGAGA CAAGACGGTC
    CGAGATGAGG GCAAGCAGCT GGCTGTTGAG ATTTACCGTT GGATCGGAGC TGCAATGAAG
    GCACAGATCT CAACGCTACC ACAGGTAACG CTAAAGGAGC TGGAGGATGA ATTCGACAAA
    CTCAAAGGTG AACGCGTTGA ACCCTCCAGA TATCTAAAGT CCCAGCAGGA AAAGCAAGCG
    AAGATCGCCG ATGCTGCAGC GACCGAGGAT GCATATAATG AGGATGATGG CGAGGCTGGC
    GTTGAAGAAA TCGATCCTAT GGATCTTCTA GACCCCGTTG ACATTCTCTC CAAAATGCCC
    AAGGACTTCT ACGATAAGCT GGAGGAAAAG AAATGGACTC TGCGCAAAGA ATCGTTGGAA
    GTCCTGGAGA AACTGCTCAC TGACCACCCC AAGCTAGAGA ATGGCGAGTA TGGAGCGCTG
    GTAAGCGCTC TTAAAAAGGT TATAACCAAG GACTCCAACG TAGTCCTCGT TGCAATGGCC
    GGAAAATGTC TAGCCTTGCT AGCCAAAGGA TTGGCAAAGC GATTCTCAAA TTACGCATCC
    GCTTGTGTCC CATCCCTGTT GGAAAAATTT AAAGAGAAGA AACCTAATGT TGTGACAGCG
    CTGCGTGAAG CTATTGATGC CATATACGCC TCCACTTCTC TAGAGGCCCA GCAAGAGTCC
    ATTGTAGAGT CCCTGTCCAA CAAAAATCCA AGTGTTAAGT CCGAAACGGC CTTGTTTATT
    GCCCGTGCCC TAACCCGCAC CCAACCAACC GCGCTAAACA AAAAGCTGCT TAAACTTCTG
    ACCACCAGTC TAGTGAAAAC GCTTAACGAA CCAGATCCAA CCGTACGCGA CAGCAGCGCC
    GAAGCTCTTG GTACTTTAAT AAAGCTAATG GGAGACAAAG CGGTGACTCC TTTGCTCGCC
    GACGTCGATC CACTCAAAAT GGCCAAGATA AAGGAGTGCC AAGAAAAGGC GGAGATTAAA
    ATAAAGGTTG CAGGTCCAAA GAAGGAGACA CGACCTGCAT CTGCACCGAC TGCGAAAGCT
    GCTGCTCCGG CTAAGACAGT GGCTGGTAGT GTGGACCCTA AGCCAGTAAC CCGACCGGCC
    ACTACAGGGG CACGTAAGGT CTTGAAAAAA CCGGCGACGG TTAGTGGAGG AGGAGCTACA
    TCTGCCCCAA CTGCAGCTCT TAAAGCGGGT GGAAAACCCC TAGCCACGGA ACGCGAAATA
    ACCCCCGAGG AATTGCAAGA AAAATCCGAA GAAATTCTGC CAGCCGAAAT TCTTAATGGG
    CTGGTGGATT CCAACTGGAA GAACCGCCTT GCTGCTGTGG AACAGCTTCT GGGCGAAATA
    AGCGGTTTTG ATGCAAAACA GGCTGGCATA TCACAGATCC TGATCCGAAC AATCAGTGGA
    CGAAAGCCAG GCCTCAAAGA AATGAATTTC CAGGTACTCA AGTTTAAGCT AGACATAATC
    CGCAGTGTGG CTGAAAACTA TCCACTGACC ACGACAACCG TTGATCTGGT GATTAACGAA
    ATAATCGAGA AGCTAGCAGA CGCCAAGAAT GGTGCAGCAG CTGCAGATGT TTTGTCTGCC
    TTTGCCGAAG CCACTAAACT GGAGTATGTT GTGGGCAAAG TATTGAGCTT TGCTTTTGAA
    CAAAAATCGC CTAAAGTGCA ATCAGAGGCC TTTAACTGGG TTAACAGGTC AATCATAGAA
    TTCGGCTTCC AATTGCAGCC AAAGACACTT ATTGAAGATG TACGAAAAGG CGTGCAGAGC
    ACGAATCCAA CGGTTCGTGC TTCCGCCATT CAAATGGTAG GCACCATGTC TATGTATATG
    GGCAAAGCTC TAATGATGTT CTTCGACAGC GAGAAGCCTG CACTAAAGTC CCAGATCCAG
    GTAGAGTTTG ACAAGAATGT GGGCGAAAAA CCACCCAAGC CTGTTAGAGG AGTTCAACGT
    AGCAGCGGTG GAACCGCTGG TAATTCCCCA GACAATGAGG ACGATGATGG TGGGGCAGCC
    GGAGAAGAGG AGCCCATTAA TATGGCTGAT CTTCTACCAC GCGTTGATAT TGCTCCACAG
    ATTACGGAGG CATTGCTGAA GGAGATGTCC GACAAGGACT GGAAAACTCG CAACGAGGGT
    CTGACTAAGC TGCAGGCTAT TATTTCAGAG GCGCGGCTAA TCAAACCCAG CATTGGCGAC
    TTGGCTCCTG CTCTGGCCCA CCGTCTGGTA GATTCCAATG CTAAGATTGC CCAAACGACA
    CTTGCCATTT GCGAGCAGCT TGCCACAGCC ATGGGGGCTG GTTGTCGAAA TCACGTGCGG
    AACTTGTTCC CTGGCTTTTT ACACGCTCTG GGTGATAACA AGAGTTTTGT AAGAGCCGCT
    GCCCTCAATT GCATCAATAG TTTTGGCGAA AAGGGCGGCT ATAAGGAATT TTTTGAGAGC
    GAAATGATAG CCGATGCCCT GAAAGGAGGA TCCCCAGCTC TAAAGACAGA ATTGTGGGCT
    TGGCTGGCAG ATAAGCTGCC TGGTCTTCCC CCCAAGTCAG TATCGAAGGA GGATATCCAT
    TCAATGGTGC CGCACTTATA TGCTCACATC TGCGATCGAA ACGCCGATGT CCGGAAAAAT
    GCCAACGAGG CGGTGTTGGG CATTATGATT CACCTTGGAT TTGATGCAAT GAACCGCGCT
    CTGGACAAAC AGAAGCCCGC GTCCAAAAAG GATATACTTG CAGCACTCGA AAAAGCGCGT
    CCTAATCTTC CGGTAAAACC GCTGCCCAAA GGCAAGCATC AGGCTCCAAT ACCTGAGGAG
    CCGAAGCTAA AAACAGTGCG CGGCGGTGGA GCAGGTGGTG CGCCTGGAAT CCAGAAGAGT
    GCTACAGCCC GGGTTGCCGG AGGACAAGAC AAGCAGGTAC CAGCGCGCAA AAAGGACGAA
    GATATCGACA CATCGCCGCT ACTGTGCGCC AACAGTGCTA AAAACCAGCG GCTGCTAGAC
    GAGCAAAAAA TGAAGGTACT AAAGTGGACT TTTGTAACCC CAAGAGAGGA ATTCACCGAA
    CTGTTACGCG ACCAAATGAT GACCGCAAAC GTAAATAAAG CACTGATAGC CAACATGTTC
    CACGACGATT TTCGCTACCA CTTGAAAGTC ATCGAGCAGT TGAGCGAAGA TCTGGCTGGT
    AACAGTAAGG CGCTGGTGTG TAATCTGGAC CTAATACTTA AATGGCTGAC GCTGCGATTT
    TACGATACGA ACCCTTCTGT GTTGATTAAG GGTCTCGAAT ATCTAGTACA GGTTTTCCAG
    GTACTCATTG ACGAGGAATA CATTCTGGCC GAGAACGAGG GAAGCAGTTT TGTCCCACAT
    CTGCTTTTAA AGATCGGAGA TCCAAAGGAT GCTGTTCGAA ATGGCGTACG CCGCGTTCTG
    CGGCAAGTAA TATTGGTCTT CCCTTTCGTC AAGGTCTTTG GCTATGTGAT GGAGGGTCTA
    AAATCGAAGA ATGCCCGTCA GCGTACTGAG TGCCTTGACG AGTTAACCTT CCTAATTGAA
    AGCTATGGCA TGAACATCTG TCCCCAATCG GCGGTGCGCG AGATTGCTCG CCAGATTTCC
    GACCGGGATA ATTCCGTACG CAACGCAGCA CTCAACTGCA TCGTCCAGGT CTTCTTTTTG
    TCCGGGGAAA AGACTTACAA GATGATTGGC CATCTTAACG AAAAGGACCT CTCAATGCTA
    GATGAGCGCA TCAAGCGAGC CAAAAAGACT AAGAAGCCCA CGCCACCGCC ATCTGTTGAT
    GTGCCGGCAC CTCAACGACA TGACAGCATC GAAATCGAAG ATGCTGAAGT GGGCAACGGT
    TGCGACGAGC TTCCACCACC AGATGAGGAC GGAACTTTCG ACCAGGCACC TTCGTCACAG
    CTGCTTCTAC TCCAGCAACA GCTTCAACAG TTACAACAGC AGGCCCAGCA ACAGAAGCCC
    AGCGGACCAT TCGGCTTGGA CTCGCAGGTA ATCAGCGAGA TCGAAAAAGA TTGGGTCCGA
    GTGGACCAAA TGGAGCAGAA GCCGCTACTG AACGTAGATA TCTCATCGCT GGACGAGCCT
    ATTAAGGTGA GGCCCACGCG GGCCGGCATT CACTACCCGC AGGAGAAGTT CGATCGGCTG
    ATTTCTCGAC AACACTATAT GCAGCAGACT TTAACGACGT CCCCGTCATC CACGGCTGGA
    ATGACCAGTG GAGTCTCCCC GTATCGCAGT CCTATGCGCT TGCAGCACCA GCAGCCTCAA
    CAGCAGCTGG AGAACAATAT ACCAAATTTG GCGGATGTTT TGCCAAAGCA CGATCCGCAG
    CTGGTAAAAG TTATAAAAGG TGTAAGCAGC ACGGACACTC TAAAGGCGCG CGCGGCTATT
    AACGAGCTTG CCGCCATCAT CGAGGCTCCA GAGAAGCAGG CTGTGCTGCG CGACTATGAA
    GAAATATTTA TTCAGAATGT GCTTGCACAG TTTAAGAATC TCTCACAAAT ACCATCGGCT
    CAATCCGTAG TTGTTTACCA GCCGCTATTA TCCATTCTGT ATACATTTTT CCATGCCAAC
    ATTCTGGGCA AAACGCTGAG CGTGGCCTGC ATCAAAAATC TAATGTCGGC GCTGTTGAAC
    CTTATGGCTG ATCCGAAGTT GGCCGTGGGT GATGATAGTC AGTATAACAA GGTTATTAAT
    GGCATATGTC TAAAAGTGTT GGACAAGGTA GATTTTACGA ATTTAAACTG TGCTTTGATA
    CGCCTATTAC GGGAGACTTG TCCGGAGGCA AAGCTGCCGA AGTTTACAGA CCTGCTGATG
    AAGTGCATAT GGCGCAACGT TAAGATGCTA CCCGAACGAA GCAACGAGCT GAACTATGAC
    GCCGTAATTC TCGAGGTGCA CGAGTTCATG CTCGCGCTGC CTAGCACTTG GTGGCAAAAC
    CGACCGTCGG ACACTCCAAT GCGCACGATT AAGACCATTC TGCATAACAT GGCAAAGGTG
    AAGGGGAATG CTATTCTGCA GCATCTCAAC CAGATTCCAA CACACTCGGA GCTACATACT
    TATTTGATAC GCATTCTCAA GAATTTCCAA AAGGATGGCT CTGCTTCAGG AATTGGTGCC
    TCCCCCCAGA GGGCCAAGGA GATTGCCTCC AAGCGAATTT CGCACCAAAC ACATGATACA
    GTATCACAGA TCTTTAAGCT GATCTCAGAT AGAGATACCA AACAGCAAGG TCTGCAGAAA
    CTTTACGACT TTAAGCAACA AAATCCTGAT ATCGATCTAA GTACCTTTCT GCAAGGCTCC
    AGTGCCCCAT TTCACAAGTA CATCGAGGAG GGTTTAGCCG AGATTGAGAG GAATCAGAAC
    GCTGGATCCA CACAGGATAA TCGGACGGCA GCTACTCGTT CTTATCTCAC AGATGTCAAC
    TACCAGAACA ACGGTCCCGA TCCCGATTTC TGGATGGACC GCCTGCAGTA TCATATGACC
    GGAGGGGCCG CCAAGCTAGC ATCTGCACGT TCAGCAGACG ATGGATCCCA TATGCTGGAC
    AATAAGGTGG TTGACGAGAA CCTCTGCCTG AACGGAATGA ACGCGCAAAA GGCGTCGCTC
    ATTAAGCGAG AAAAGCGGGA CATGTCACCA AATCGCTTGC AGCATCTCCA GGCAAAGCTG
    GCTCAAATCA AGAAAGAGAA TCATGCCCAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001247131.1
    CDS110..6262
    Translation

    Target ORF information:

    RefSeq Version NM_001260202.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster mini spindles (msps), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001260202.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCGAGG ACACAGAGTA CAAGAAGTTG CCGGTGGAGG AGCGGTGTGT CCATAAACTA 
    TGGAAAGCCC GCGTCGATGG TTACGAGGAG GCCGCAAAGA TCTTTCGCGA GCTGGATGAC
    GAGAAGTCGC CGGAATGGTC TAAATTTGCA GGGCTCATAA AGAAGATGGT AGTGGACTCT
    AATGCTCTGG CCCAGGAAAA GGGTCTAGAA GCGGCCCTCA TTTTTGTGGA GAACAGCGGG
    CTGGCGGGAC GCACAGTAGG CGACGTGATG ACAGGCATCG TTCAGAAGTG CATTGCGGCG
    CCAAAGACCA AAACCAAGGA GCTCTCCGTC CAAGTGGCTC TAATGTATGT GGAGATAGAG
    AAGCAGGAGG CCGTCGTCGA AGAGCTGGTT AAAGGTATGG AGGCCAAGAA CCCTAAGATT
    GTGTCTGCCT GCGTGGCAGC CACCACACTG GCCCTCCGCG AGTTCGGACA CAAGGTGATC
    GGCGTCAAGC CGTTAATTAA AAAGCTTGCT CCGCTAATGT CTGACCGAGA CAAGACGGTC
    CGAGATGAGG GCAAGCAGCT GGCTGTTGAG ATTTACCGTT GGATCGGAGC TGCAATGAAG
    GCACAGATCT CAACGCTACC ACAGGTAACG CTAAAGGAGC TGGAGGATGA ATTCGACAAA
    CTCAAAGGTG AACGCGTTGA ACCCTCCAGA TATCTAAAGT CCCAGCAGGA AAAGCAAGCG
    AAGATCGCCG ATGCTGCAGC GACCGAGGAT GCATATAATG AGGATGATGG CGAGGCTGGC
    GTTGAAGAAA TCGATCCTAT GGATCTTCTA GACCCCGTTG ACATTCTCTC CAAAATGCCC
    AAGGACTTCT ACGATAAGCT GGAGGAAAAG AAATGGACTC TGCGCAAAGA ATCGTTGGAA
    GTCCTGGAGA AACTGCTCAC TGACCACCCC AAGCTAGAGA ATGGCGAGTA TGGAGCGCTG
    GTAAGCGCTC TTAAAAAGGT TATAACCAAG GACTCCAACG TAGTCCTCGT TGCAATGGCC
    GGAAAATGTC TAGCCTTGCT AGCCAAAGGA TTGGCAAAGC GATTCTCAAA TTACGCATCC
    GCTTGTGTCC CATCCCTGTT GGAAAAATTT AAAGAGAAGA AACCTAATGT TGTGACAGCG
    CTGCGTGAAG CTATTGATGC CATATACGCC TCCACTTCTC TAGAGGCCCA GCAAGAGTCC
    ATTGTAGAGT CCCTGTCCAA CAAAAATCCA AGTGTTAAGT CCGAAACGGC CTTGTTTATT
    GCCCGTGCCC TAACCCGCAC CCAACCAACC GCGCTAAACA AAAAGCTGCT TAAACTTCTG
    ACCACCAGTC TAGTGAAAAC GCTTAACGAA CCAGATCCAA CCGTACGCGA CAGCAGCGCC
    GAAGCTCTTG GTACTTTAAT AAAGCTAATG GGAGACAAAG CGGTGACTCC TTTGCTCGCC
    GACGTCGATC CACTCAAAAT GGCCAAGATA AAGGAGTGCC AAGAAAAGGC GGAGATTAAA
    ATAAAGGTTG CAGGTCCAAA GAAGGAGACA CGACCTGCAT CTGCACCGAC TGCGAAAGCT
    GCTGCTCCGG CTAAGACAGT GGCTGGTAGT GTGGACCCTA AGCCAGTAAC CCGACCGGCC
    ACTACAGGGG CACGTAAGGT CTTGAAAAAA CCGGCGACGG TTAGTGGAGG AGGAGCTACA
    TCTGCCCCAA CTGCAGCTCT TAAAGCGGGT GGAAAACCCC TAGCCACGGA ACGCGAAATA
    ACCCCCGAGG AATTGCAAGA AAAATCCGAA GAAATTCTGC CAGCCGAAAT TCTTAATGGG
    CTGGTGGATT CCAACTGGAA GAACCGCCTT GCTGCTGTGG AACAGCTTCT GGGCGAAATA
    AGCGGTTTTG ATGCAAAACA GGCTGGCATA TCACAGATCC TGATCCGAAC AATCAGTGGA
    CGAAAGCCAG GCCTCAAAGA AATGAATTTC CAGGTACTCA AGTTTAAGCT AGACATAATC
    CGCAGTGTGG CTGAAAACTA TCCACTGACC ACGACAACCG TTGATCTGGT GATTAACGAA
    ATAATCGAGA AGCTAGCAGA CGCCAAGAAT GGTGCAGCAG CTGCAGATGT TTTGTCTGCC
    TTTGCCGAAG CCACTAAACT GGAGTATGTT GTGGGCAAAG TATTGAGCTT TGCTTTTGAA
    CAAAAATCGC CTAAAGTGCA ATCAGAGGCC TTTAACTGGG TTAACAGGTC AATCATAGAA
    TTCGGCTTCC AATTGCAGCC AAAGACACTT ATTGAAGATG TACGAAAAGG CGTGCAGAGC
    ACGAATCCAA CGGTTCGTGC TTCCGCCATT CAAATGGTAG GCACCATGTC TATGTATATG
    GGCAAAGCTC TAATGATGTT CTTCGACAGC GAGAAGCCTG CACTAAAGTC CCAGATCCAG
    GTAGAGTTTG ACAAGAATGT GGGCGAAAAA CCACCCAAGC CTGTTAGAGG AGTTCAACGT
    AGCAGCGGTG GAACCGCTGG TAATTCCCCA GACAATGAGG ACGATGATGG TGGGGCAGCC
    GGAGAAGAGG AGCCCATTAA TATGGCTGAT CTTCTACCAC GCGTTGATAT TGCTCCACAG
    ATTACGGAGG CATTGCTGAA GGAGATGTCC GACAAGGACT GGAAAACTCG CAACGAGGGT
    CTGACTAAGC TGCAGGCTAT TATTTCAGAG GCGCGGCTAA TCAAACCCAG CATTGGCGAC
    TTGGCTCCTG CTCTGGCCCA CCGTCTGGTA GATTCCAATG CTAAGATTGC CCAAACGACA
    CTTGCCATTT GCGAGCAGCT TGCCACAGCC ATGGGGGCTG GTTGTCGAAA TCACGTGCGG
    AACTTGTTCC CTGGCTTTTT ACACGCTCTG GGTGATAACA AGAGTTTTGT AAGAGCCGCT
    GCCCTCAATT GCATCAATAG TTTTGGCGAA AAGGGCGGCT ATAAGGAATT TTTTGAGAGC
    GAAATGATAG CCGATGCCCT GAAAGGAGGA TCCCCAGCTC TAAAGACAGA ATTGTGGGCT
    TGGCTGGCAG ATAAGCTGCC TGGTCTTCCC CCCAAGTCAG TATCGAAGGA GGATATCCAT
    TCAATGGTGC CGCACTTATA TGCTCACATC TGCGATCGAA ACGCCGATGT CCGGAAAAAT
    GCCAACGAGG CGGTGTTGGG CATTATGATT CACCTTGGAT TTGATGCAAT GAACCGCGCT
    CTGGACAAAC AGAAGCCCGC GTCCAAAAAG GATATACTTG CAGCACTCGA AAAAGCGCGT
    CCTAATCTTC CGGTAAAACC GCTGCCCAAA GGCAAGCATC AGGCTCCAAT ACCTGAGGAG
    CCGAAGCTAA AAACAGTGCG CGGCGGTGGA GCAGGTGGTG CGCCTGGAAT CCAGAAGAGT
    GCTACAGCCC GGGTTGCCGG AGGACAAGAC AAGCAGGTAC CAGCGCGCAA AAAGGACGAA
    GATATCGACA CATCGCCGCT ACTGTGCGCC AACAGTGCTA AAAACCAGCG GCTGCTAGAC
    GAGCAAAAAA TGAAGGTACT AAAGTGGACT TTTGTAACCC CAAGAGAGGA ATTCACCGAA
    CTGTTACGCG ACCAAATGAT GACCGCAAAC GTAAATAAAG CACTGATAGC CAACATGTTC
    CACGACGATT TTCGCTACCA CTTGAAAGTC ATCGAGCAGT TGAGCGAAGA TCTGGCTGGT
    AACAGTAAGG CGCTGGTGTG TAATCTGGAC CTAATACTTA AATGGCTGAC GCTGCGATTT
    TACGATACGA ACCCTTCTGT GTTGATTAAG GGTCTCGAAT ATCTAGTACA GGTTTTCCAG
    GTACTCATTG ACGAGGAATA CATTCTGGCC GAGAACGAGG GAAGCAGTTT TGTCCCACAT
    CTGCTTTTAA AGATCGGAGA TCCAAAGGAT GCTGTTCGAA ATGGCGTACG CCGCGTTCTG
    CGGCAAGTAA TATTGGTCTT CCCTTTCGTC AAGGTCTTTG GCTATGTGAT GGAGGGTCTA
    AAATCGAAGA ATGCCCGTCA GCGTACTGAG TGCCTTGACG AGTTAACCTT CCTAATTGAA
    AGCTATGGCA TGAACATCTG TCCCCAATCG GCGGTGCGCG AGATTGCTCG CCAGATTTCC
    GACCGGGATA ATTCCGTACG CAACGCAGCA CTCAACTGCA TCGTCCAGGT CTTCTTTTTG
    TCCGGGGAAA AGACTTACAA GATGATTGGC CATCTTAACG AAAAGGACCT CTCAATGCTA
    GATGAGCGCA TCAAGCGAGC CAAAAAGACT AAGAAGCCCA CGCCACCGCC ATCTGTTGAT
    GTGCCGGCAC CTCAACGACA TGACAGCATC GAAATCGAAG ATGCTGAAGT GGGCAACGGT
    TGCGACGAGC TTCCACCACC AGATGAGGAC GGAACTTTCG ACCAGGCACC TTCGTCACAG
    CTGCTTCTAC TCCAGCAACA GCTTCAACAG TTACAACAGC AGGCCCAGCA ACAGAAGCCC
    AGCGGACCAT TCGGCTTGGA CTCGCAGGTA ATCAGCGAGA TCGAAAAAGA TTGGGTCCGA
    GTGGACCAAA TGGAGCAGAA GCCGCTACTG AACGTAGATA TCTCATCGCT GGACGAGCCT
    ATTAAGGTGA GGCCCACGCG GGCCGGCATT CACTACCCGC AGGAGAAGTT CGATCGGCTG
    ATTTCTCGAC AACACTATAT GCAGCAGACT TTAACGACGT CCCCGTCATC CACGGCTGGA
    ATGACCAGTG GAGTCTCCCC GTATCGCAGT CCTATGCGCT TGCAGCACCA GCAGCCTCAA
    CAGCAGCTGG AGAACAATAT ACCAAATTTG GCGGATGTTT TGCCAAAGCA CGATCCGCAG
    CTGGTAAAAG TTATAAAAGG TGTAAGCAGC ACGGACACTC TAAAGGCGCG CGCGGCTATT
    AACGAGCTTG CCGCCATCAT CGAGGCTCCA GAGAAGCAGG CTGTGCTGCG CGACTATGAA
    GAAATATTTA TTCAGAATGT GCTTGCACAG TTTAAGAATC TCTCACAAAT ACCATCGGCT
    CAATCCGTAG TTGTTTACCA GCCGCTATTA TCCATTCTGT ATACATTTTT CCATGCCAAC
    ATTCTGGGCA AAACGCTGAG CGTGGCCTGC ATCAAAAATC TAATGTCGGC GCTGTTGAAC
    CTTATGGCTG ATCCGAAGTT GGCCGTGGGT GATGATAGTC AGTATAACAA GGTTATTAAT
    GGCATATGTC TAAAAGTGTT GGACAAGGTA GATTTTACGA ATTTAAACTG TGCTTTGATA
    CGCCTATTAC GGGAGACTTG TCCGGAGGCA AAGCTGCCGA AGTTTACAGA CCTGCTGATG
    AAGTGCATAT GGCGCAACGT TAAGATGCTA CCCGAACGAA GCAACGAGCT GAACTATGAC
    GCCGTAATTC TCGAGGTGCA CGAGTTCATG CTCGCGCTGC CTAGCACTTG GTGGCAAAAC
    CGACCGTCGG ACACTCCAAT GCGCACGATT AAGACCATTC TGCATAACAT GGCAAAGGTG
    AAGGGGAATG CTATTCTGCA GCATCTCAAC CAGATTCCAA CACACTCGGA GCTACATACT
    TATTTGATAC GCATTCTCAA GAATTTCCAA AAGGATGGCT CTGCTTCAGG AATTGGTGCC
    TCCCCCCAGA GGGCCAAGGA GATTGCCTCC AAGCGAATTT CGCACCAAAC ACATGATACA
    GTATCACAGA TCTTTAAGCT GATCTCAGAT AGAGATACCA AACAGCAAGG TCTGCAGAAA
    CTTTACGACT TTAAGCAACA AAATCCTGAT ATCGATCTAA GTACCTTTCT GCAAGGCTCC
    AGTGCCCCAT TTCACAAGTA CATCGAGGAG GGTTTAGCCG AGATTGAGAG GAATCAGAAC
    GCTGGATCCA CACAGGATAA TCGGACGGCA GCTACTCGTT CTTATCTCAC AGATGTCAAC
    TACCAGAACA ACGGTCCCGA TCCCGATTTC TGGATGGACC GCCTGCAGTA TCATATGACC
    GGAGGGGCCG CCAAGCTAGC ATCTGCACGT TCAGCAGACG ATGGATCCCA TATGCTGGAC
    AATAAGGTGG TTGACGAGAA CCTCTGCCTG AACGGAATGA ACGCGCAAAA GGCGTCGCTC
    ATTAAGCGAG AAAAGCGGGA CATGTCACCA AATCGCTTGC AGCATCTCCA GGCAAAGCTG
    GCTCAAATCA AGAAAGAGAA TCATGCCCAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08421
    Clone ID Related Accession (Same CDS sequence) NM_001260202.1 , NM_001170156.2
    Accession Version NM_001170156.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6153bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product mini spindles, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170156.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCGAGG ACACAGAGTA CAAGAAGTTG CCGGTGGAGG AGCGGTGTGT CCATAAACTA 
    TGGAAAGCCC GCGTCGATGG TTACGAGGAG GCCGCAAAGA TCTTTCGCGA GCTGGATGAC
    GAGAAGTCGC CGGAATGGTC TAAATTTGCA GGGCTCATAA AGAAGATGGT AGTGGACTCT
    AATGCTCTGG CCCAGGAAAA GGGTCTAGAA GCGGCCCTCA TTTTTGTGGA GAACAGCGGG
    CTGGCGGGAC GCACAGTAGG CGACGTGATG ACAGGCATCG TTCAGAAGTG CATTGCGGCG
    CCAAAGACCA AAACCAAGGA GCTCTCCGTC CAAGTGGCTC TAATGTATGT GGAGATAGAG
    AAGCAGGAGG CCGTCGTCGA AGAGCTGGTT AAAGGTATGG AGGCCAAGAA CCCTAAGATT
    GTGTCTGCCT GCGTGGCAGC CACCACACTG GCCCTCCGCG AGTTCGGACA CAAGGTGATC
    GGCGTCAAGC CGTTAATTAA AAAGCTTGCT CCGCTAATGT CTGACCGAGA CAAGACGGTC
    CGAGATGAGG GCAAGCAGCT GGCTGTTGAG ATTTACCGTT GGATCGGAGC TGCAATGAAG
    GCACAGATCT CAACGCTACC ACAGGTAACG CTAAAGGAGC TGGAGGATGA ATTCGACAAA
    CTCAAAGGTG AACGCGTTGA ACCCTCCAGA TATCTAAAGT CCCAGCAGGA AAAGCAAGCG
    AAGATCGCCG ATGCTGCAGC GACCGAGGAT GCATATAATG AGGATGATGG CGAGGCTGGC
    GTTGAAGAAA TCGATCCTAT GGATCTTCTA GACCCCGTTG ACATTCTCTC CAAAATGCCC
    AAGGACTTCT ACGATAAGCT GGAGGAAAAG AAATGGACTC TGCGCAAAGA ATCGTTGGAA
    GTCCTGGAGA AACTGCTCAC TGACCACCCC AAGCTAGAGA ATGGCGAGTA TGGAGCGCTG
    GTAAGCGCTC TTAAAAAGGT TATAACCAAG GACTCCAACG TAGTCCTCGT TGCAATGGCC
    GGAAAATGTC TAGCCTTGCT AGCCAAAGGA TTGGCAAAGC GATTCTCAAA TTACGCATCC
    GCTTGTGTCC CATCCCTGTT GGAAAAATTT AAAGAGAAGA AACCTAATGT TGTGACAGCG
    CTGCGTGAAG CTATTGATGC CATATACGCC TCCACTTCTC TAGAGGCCCA GCAAGAGTCC
    ATTGTAGAGT CCCTGTCCAA CAAAAATCCA AGTGTTAAGT CCGAAACGGC CTTGTTTATT
    GCCCGTGCCC TAACCCGCAC CCAACCAACC GCGCTAAACA AAAAGCTGCT TAAACTTCTG
    ACCACCAGTC TAGTGAAAAC GCTTAACGAA CCAGATCCAA CCGTACGCGA CAGCAGCGCC
    GAAGCTCTTG GTACTTTAAT AAAGCTAATG GGAGACAAAG CGGTGACTCC TTTGCTCGCC
    GACGTCGATC CACTCAAAAT GGCCAAGATA AAGGAGTGCC AAGAAAAGGC GGAGATTAAA
    ATAAAGGTTG CAGGTCCAAA GAAGGAGACA CGACCTGCAT CTGCACCGAC TGCGAAAGCT
    GCTGCTCCGG CTAAGACAGT GGCTGGTAGT GTGGACCCTA AGCCAGTAAC CCGACCGGCC
    ACTACAGGGG CACGTAAGGT CTTGAAAAAA CCGGCGACGG TTAGTGGAGG AGGAGCTACA
    TCTGCCCCAA CTGCAGCTCT TAAAGCGGGT GGAAAACCCC TAGCCACGGA ACGCGAAATA
    ACCCCCGAGG AATTGCAAGA AAAATCCGAA GAAATTCTGC CAGCCGAAAT TCTTAATGGG
    CTGGTGGATT CCAACTGGAA GAACCGCCTT GCTGCTGTGG AACAGCTTCT GGGCGAAATA
    AGCGGTTTTG ATGCAAAACA GGCTGGCATA TCACAGATCC TGATCCGAAC AATCAGTGGA
    CGAAAGCCAG GCCTCAAAGA AATGAATTTC CAGGTACTCA AGTTTAAGCT AGACATAATC
    CGCAGTGTGG CTGAAAACTA TCCACTGACC ACGACAACCG TTGATCTGGT GATTAACGAA
    ATAATCGAGA AGCTAGCAGA CGCCAAGAAT GGTGCAGCAG CTGCAGATGT TTTGTCTGCC
    TTTGCCGAAG CCACTAAACT GGAGTATGTT GTGGGCAAAG TATTGAGCTT TGCTTTTGAA
    CAAAAATCGC CTAAAGTGCA ATCAGAGGCC TTTAACTGGG TTAACAGGTC AATCATAGAA
    TTCGGCTTCC AATTGCAGCC AAAGACACTT ATTGAAGATG TACGAAAAGG CGTGCAGAGC
    ACGAATCCAA CGGTTCGTGC TTCCGCCATT CAAATGGTAG GCACCATGTC TATGTATATG
    GGCAAAGCTC TAATGATGTT CTTCGACAGC GAGAAGCCTG CACTAAAGTC CCAGATCCAG
    GTAGAGTTTG ACAAGAATGT GGGCGAAAAA CCACCCAAGC CTGTTAGAGG AGTTCAACGT
    AGCAGCGGTG GAACCGCTGG TAATTCCCCA GACAATGAGG ACGATGATGG TGGGGCAGCC
    GGAGAAGAGG AGCCCATTAA TATGGCTGAT CTTCTACCAC GCGTTGATAT TGCTCCACAG
    ATTACGGAGG CATTGCTGAA GGAGATGTCC GACAAGGACT GGAAAACTCG CAACGAGGGT
    CTGACTAAGC TGCAGGCTAT TATTTCAGAG GCGCGGCTAA TCAAACCCAG CATTGGCGAC
    TTGGCTCCTG CTCTGGCCCA CCGTCTGGTA GATTCCAATG CTAAGATTGC CCAAACGACA
    CTTGCCATTT GCGAGCAGCT TGCCACAGCC ATGGGGGCTG GTTGTCGAAA TCACGTGCGG
    AACTTGTTCC CTGGCTTTTT ACACGCTCTG GGTGATAACA AGAGTTTTGT AAGAGCCGCT
    GCCCTCAATT GCATCAATAG TTTTGGCGAA AAGGGCGGCT ATAAGGAATT TTTTGAGAGC
    GAAATGATAG CCGATGCCCT GAAAGGAGGA TCCCCAGCTC TAAAGACAGA ATTGTGGGCT
    TGGCTGGCAG ATAAGCTGCC TGGTCTTCCC CCCAAGTCAG TATCGAAGGA GGATATCCAT
    TCAATGGTGC CGCACTTATA TGCTCACATC TGCGATCGAA ACGCCGATGT CCGGAAAAAT
    GCCAACGAGG CGGTGTTGGG CATTATGATT CACCTTGGAT TTGATGCAAT GAACCGCGCT
    CTGGACAAAC AGAAGCCCGC GTCCAAAAAG GATATACTTG CAGCACTCGA AAAAGCGCGT
    CCTAATCTTC CGGTAAAACC GCTGCCCAAA GGCAAGCATC AGGCTCCAAT ACCTGAGGAG
    CCGAAGCTAA AAACAGTGCG CGGCGGTGGA GCAGGTGGTG CGCCTGGAAT CCAGAAGAGT
    GCTACAGCCC GGGTTGCCGG AGGACAAGAC AAGCAGGTAC CAGCGCGCAA AAAGGACGAA
    GATATCGACA CATCGCCGCT ACTGTGCGCC AACAGTGCTA AAAACCAGCG GCTGCTAGAC
    GAGCAAAAAA TGAAGGTACT AAAGTGGACT TTTGTAACCC CAAGAGAGGA ATTCACCGAA
    CTGTTACGCG ACCAAATGAT GACCGCAAAC GTAAATAAAG CACTGATAGC CAACATGTTC
    CACGACGATT TTCGCTACCA CTTGAAAGTC ATCGAGCAGT TGAGCGAAGA TCTGGCTGGT
    AACAGTAAGG CGCTGGTGTG TAATCTGGAC CTAATACTTA AATGGCTGAC GCTGCGATTT
    TACGATACGA ACCCTTCTGT GTTGATTAAG GGTCTCGAAT ATCTAGTACA GGTTTTCCAG
    GTACTCATTG ACGAGGAATA CATTCTGGCC GAGAACGAGG GAAGCAGTTT TGTCCCACAT
    CTGCTTTTAA AGATCGGAGA TCCAAAGGAT GCTGTTCGAA ATGGCGTACG CCGCGTTCTG
    CGGCAAGTAA TATTGGTCTT CCCTTTCGTC AAGGTCTTTG GCTATGTGAT GGAGGGTCTA
    AAATCGAAGA ATGCCCGTCA GCGTACTGAG TGCCTTGACG AGTTAACCTT CCTAATTGAA
    AGCTATGGCA TGAACATCTG TCCCCAATCG GCGGTGCGCG AGATTGCTCG CCAGATTTCC
    GACCGGGATA ATTCCGTACG CAACGCAGCA CTCAACTGCA TCGTCCAGGT CTTCTTTTTG
    TCCGGGGAAA AGACTTACAA GATGATTGGC CATCTTAACG AAAAGGACCT CTCAATGCTA
    GATGAGCGCA TCAAGCGAGC CAAAAAGACT AAGAAGCCCA CGCCACCGCC ATCTGTTGAT
    GTGCCGGCAC CTCAACGACA TGACAGCATC GAAATCGAAG ATGCTGAAGT GGGCAACGGT
    TGCGACGAGC TTCCACCACC AGATGAGGAC GGAACTTTCG ACCAGGCACC TTCGTCACAG
    CTGCTTCTAC TCCAGCAACA GCTTCAACAG TTACAACAGC AGGCCCAGCA ACAGAAGCCC
    AGCGGACCAT TCGGCTTGGA CTCGCAGGTA ATCAGCGAGA TCGAAAAAGA TTGGGTCCGA
    GTGGACCAAA TGGAGCAGAA GCCGCTACTG AACGTAGATA TCTCATCGCT GGACGAGCCT
    ATTAAGGTGA GGCCCACGCG GGCCGGCATT CACTACCCGC AGGAGAAGTT CGATCGGCTG
    ATTTCTCGAC AACACTATAT GCAGCAGACT TTAACGACGT CCCCGTCATC CACGGCTGGA
    ATGACCAGTG GAGTCTCCCC GTATCGCAGT CCTATGCGCT TGCAGCACCA GCAGCCTCAA
    CAGCAGCTGG AGAACAATAT ACCAAATTTG GCGGATGTTT TGCCAAAGCA CGATCCGCAG
    CTGGTAAAAG TTATAAAAGG TGTAAGCAGC ACGGACACTC TAAAGGCGCG CGCGGCTATT
    AACGAGCTTG CCGCCATCAT CGAGGCTCCA GAGAAGCAGG CTGTGCTGCG CGACTATGAA
    GAAATATTTA TTCAGAATGT GCTTGCACAG TTTAAGAATC TCTCACAAAT ACCATCGGCT
    CAATCCGTAG TTGTTTACCA GCCGCTATTA TCCATTCTGT ATACATTTTT CCATGCCAAC
    ATTCTGGGCA AAACGCTGAG CGTGGCCTGC ATCAAAAATC TAATGTCGGC GCTGTTGAAC
    CTTATGGCTG ATCCGAAGTT GGCCGTGGGT GATGATAGTC AGTATAACAA GGTTATTAAT
    GGCATATGTC TAAAAGTGTT GGACAAGGTA GATTTTACGA ATTTAAACTG TGCTTTGATA
    CGCCTATTAC GGGAGACTTG TCCGGAGGCA AAGCTGCCGA AGTTTACAGA CCTGCTGATG
    AAGTGCATAT GGCGCAACGT TAAGATGCTA CCCGAACGAA GCAACGAGCT GAACTATGAC
    GCCGTAATTC TCGAGGTGCA CGAGTTCATG CTCGCGCTGC CTAGCACTTG GTGGCAAAAC
    CGACCGTCGG ACACTCCAAT GCGCACGATT AAGACCATTC TGCATAACAT GGCAAAGGTG
    AAGGGGAATG CTATTCTGCA GCATCTCAAC CAGATTCCAA CACACTCGGA GCTACATACT
    TATTTGATAC GCATTCTCAA GAATTTCCAA AAGGATGGCT CTGCTTCAGG AATTGGTGCC
    TCCCCCCAGA GGGCCAAGGA GATTGCCTCC AAGCGAATTT CGCACCAAAC ACATGATACA
    GTATCACAGA TCTTTAAGCT GATCTCAGAT AGAGATACCA AACAGCAAGG TCTGCAGAAA
    CTTTACGACT TTAAGCAACA AAATCCTGAT ATCGATCTAA GTACCTTTCT GCAAGGCTCC
    AGTGCCCCAT TTCACAAGTA CATCGAGGAG GGTTTAGCCG AGATTGAGAG GAATCAGAAC
    GCTGGATCCA CACAGGATAA TCGGACGGCA GCTACTCGTT CTTATCTCAC AGATGTCAAC
    TACCAGAACA ACGGTCCCGA TCCCGATTTC TGGATGGACC GCCTGCAGTA TCATATGACC
    GGAGGGGCCG CCAAGCTAGC ATCTGCACGT TCAGCAGACG ATGGATCCCA TATGCTGGAC
    AATAAGGTGG TTGACGAGAA CCTCTGCCTG AACGGAATGA ACGCGCAAAA GGCGTCGCTC
    ATTAAGCGAG AAAAGCGGGA CATGTCACCA AATCGCTTGC AGCATCTCCA GGCAAAGCTG
    GCTCAAATCA AGAAAGAGAA TCATGCCCAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163627.1
    CDS447..6599
    Translation

    Target ORF information:

    RefSeq Version NM_001170156.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster mini spindles (msps), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001170156.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCCGAGG ACACAGAGTA CAAGAAGTTG CCGGTGGAGG AGCGGTGTGT CCATAAACTA 
    TGGAAAGCCC GCGTCGATGG TTACGAGGAG GCCGCAAAGA TCTTTCGCGA GCTGGATGAC
    GAGAAGTCGC CGGAATGGTC TAAATTTGCA GGGCTCATAA AGAAGATGGT AGTGGACTCT
    AATGCTCTGG CCCAGGAAAA GGGTCTAGAA GCGGCCCTCA TTTTTGTGGA GAACAGCGGG
    CTGGCGGGAC GCACAGTAGG CGACGTGATG ACAGGCATCG TTCAGAAGTG CATTGCGGCG
    CCAAAGACCA AAACCAAGGA GCTCTCCGTC CAAGTGGCTC TAATGTATGT GGAGATAGAG
    AAGCAGGAGG CCGTCGTCGA AGAGCTGGTT AAAGGTATGG AGGCCAAGAA CCCTAAGATT
    GTGTCTGCCT GCGTGGCAGC CACCACACTG GCCCTCCGCG AGTTCGGACA CAAGGTGATC
    GGCGTCAAGC CGTTAATTAA AAAGCTTGCT CCGCTAATGT CTGACCGAGA CAAGACGGTC
    CGAGATGAGG GCAAGCAGCT GGCTGTTGAG ATTTACCGTT GGATCGGAGC TGCAATGAAG
    GCACAGATCT CAACGCTACC ACAGGTAACG CTAAAGGAGC TGGAGGATGA ATTCGACAAA
    CTCAAAGGTG AACGCGTTGA ACCCTCCAGA TATCTAAAGT CCCAGCAGGA AAAGCAAGCG
    AAGATCGCCG ATGCTGCAGC GACCGAGGAT GCATATAATG AGGATGATGG CGAGGCTGGC
    GTTGAAGAAA TCGATCCTAT GGATCTTCTA GACCCCGTTG ACATTCTCTC CAAAATGCCC
    AAGGACTTCT ACGATAAGCT GGAGGAAAAG AAATGGACTC TGCGCAAAGA ATCGTTGGAA
    GTCCTGGAGA AACTGCTCAC TGACCACCCC AAGCTAGAGA ATGGCGAGTA TGGAGCGCTG
    GTAAGCGCTC TTAAAAAGGT TATAACCAAG GACTCCAACG TAGTCCTCGT TGCAATGGCC
    GGAAAATGTC TAGCCTTGCT AGCCAAAGGA TTGGCAAAGC GATTCTCAAA TTACGCATCC
    GCTTGTGTCC CATCCCTGTT GGAAAAATTT AAAGAGAAGA AACCTAATGT TGTGACAGCG
    CTGCGTGAAG CTATTGATGC CATATACGCC TCCACTTCTC TAGAGGCCCA GCAAGAGTCC
    ATTGTAGAGT CCCTGTCCAA CAAAAATCCA AGTGTTAAGT CCGAAACGGC CTTGTTTATT
    GCCCGTGCCC TAACCCGCAC CCAACCAACC GCGCTAAACA AAAAGCTGCT TAAACTTCTG
    ACCACCAGTC TAGTGAAAAC GCTTAACGAA CCAGATCCAA CCGTACGCGA CAGCAGCGCC
    GAAGCTCTTG GTACTTTAAT AAAGCTAATG GGAGACAAAG CGGTGACTCC TTTGCTCGCC
    GACGTCGATC CACTCAAAAT GGCCAAGATA AAGGAGTGCC AAGAAAAGGC GGAGATTAAA
    ATAAAGGTTG CAGGTCCAAA GAAGGAGACA CGACCTGCAT CTGCACCGAC TGCGAAAGCT
    GCTGCTCCGG CTAAGACAGT GGCTGGTAGT GTGGACCCTA AGCCAGTAAC CCGACCGGCC
    ACTACAGGGG CACGTAAGGT CTTGAAAAAA CCGGCGACGG TTAGTGGAGG AGGAGCTACA
    TCTGCCCCAA CTGCAGCTCT TAAAGCGGGT GGAAAACCCC TAGCCACGGA ACGCGAAATA
    ACCCCCGAGG AATTGCAAGA AAAATCCGAA GAAATTCTGC CAGCCGAAAT TCTTAATGGG
    CTGGTGGATT CCAACTGGAA GAACCGCCTT GCTGCTGTGG AACAGCTTCT GGGCGAAATA
    AGCGGTTTTG ATGCAAAACA GGCTGGCATA TCACAGATCC TGATCCGAAC AATCAGTGGA
    CGAAAGCCAG GCCTCAAAGA AATGAATTTC CAGGTACTCA AGTTTAAGCT AGACATAATC
    CGCAGTGTGG CTGAAAACTA TCCACTGACC ACGACAACCG TTGATCTGGT GATTAACGAA
    ATAATCGAGA AGCTAGCAGA CGCCAAGAAT GGTGCAGCAG CTGCAGATGT TTTGTCTGCC
    TTTGCCGAAG CCACTAAACT GGAGTATGTT GTGGGCAAAG TATTGAGCTT TGCTTTTGAA
    CAAAAATCGC CTAAAGTGCA ATCAGAGGCC TTTAACTGGG TTAACAGGTC AATCATAGAA
    TTCGGCTTCC AATTGCAGCC AAAGACACTT ATTGAAGATG TACGAAAAGG CGTGCAGAGC
    ACGAATCCAA CGGTTCGTGC TTCCGCCATT CAAATGGTAG GCACCATGTC TATGTATATG
    GGCAAAGCTC TAATGATGTT CTTCGACAGC GAGAAGCCTG CACTAAAGTC CCAGATCCAG
    GTAGAGTTTG ACAAGAATGT GGGCGAAAAA CCACCCAAGC CTGTTAGAGG AGTTCAACGT
    AGCAGCGGTG GAACCGCTGG TAATTCCCCA GACAATGAGG ACGATGATGG TGGGGCAGCC
    GGAGAAGAGG AGCCCATTAA TATGGCTGAT CTTCTACCAC GCGTTGATAT TGCTCCACAG
    ATTACGGAGG CATTGCTGAA GGAGATGTCC GACAAGGACT GGAAAACTCG CAACGAGGGT
    CTGACTAAGC TGCAGGCTAT TATTTCAGAG GCGCGGCTAA TCAAACCCAG CATTGGCGAC
    TTGGCTCCTG CTCTGGCCCA CCGTCTGGTA GATTCCAATG CTAAGATTGC CCAAACGACA
    CTTGCCATTT GCGAGCAGCT TGCCACAGCC ATGGGGGCTG GTTGTCGAAA TCACGTGCGG
    AACTTGTTCC CTGGCTTTTT ACACGCTCTG GGTGATAACA AGAGTTTTGT AAGAGCCGCT
    GCCCTCAATT GCATCAATAG TTTTGGCGAA AAGGGCGGCT ATAAGGAATT TTTTGAGAGC
    GAAATGATAG CCGATGCCCT GAAAGGAGGA TCCCCAGCTC TAAAGACAGA ATTGTGGGCT
    TGGCTGGCAG ATAAGCTGCC TGGTCTTCCC CCCAAGTCAG TATCGAAGGA GGATATCCAT
    TCAATGGTGC CGCACTTATA TGCTCACATC TGCGATCGAA ACGCCGATGT CCGGAAAAAT
    GCCAACGAGG CGGTGTTGGG CATTATGATT CACCTTGGAT TTGATGCAAT GAACCGCGCT
    CTGGACAAAC AGAAGCCCGC GTCCAAAAAG GATATACTTG CAGCACTCGA AAAAGCGCGT
    CCTAATCTTC CGGTAAAACC GCTGCCCAAA GGCAAGCATC AGGCTCCAAT ACCTGAGGAG
    CCGAAGCTAA AAACAGTGCG CGGCGGTGGA GCAGGTGGTG CGCCTGGAAT CCAGAAGAGT
    GCTACAGCCC GGGTTGCCGG AGGACAAGAC AAGCAGGTAC CAGCGCGCAA AAAGGACGAA
    GATATCGACA CATCGCCGCT ACTGTGCGCC AACAGTGCTA AAAACCAGCG GCTGCTAGAC
    GAGCAAAAAA TGAAGGTACT AAAGTGGACT TTTGTAACCC CAAGAGAGGA ATTCACCGAA
    CTGTTACGCG ACCAAATGAT GACCGCAAAC GTAAATAAAG CACTGATAGC CAACATGTTC
    CACGACGATT TTCGCTACCA CTTGAAAGTC ATCGAGCAGT TGAGCGAAGA TCTGGCTGGT
    AACAGTAAGG CGCTGGTGTG TAATCTGGAC CTAATACTTA AATGGCTGAC GCTGCGATTT
    TACGATACGA ACCCTTCTGT GTTGATTAAG GGTCTCGAAT ATCTAGTACA GGTTTTCCAG
    GTACTCATTG ACGAGGAATA CATTCTGGCC GAGAACGAGG GAAGCAGTTT TGTCCCACAT
    CTGCTTTTAA AGATCGGAGA TCCAAAGGAT GCTGTTCGAA ATGGCGTACG CCGCGTTCTG
    CGGCAAGTAA TATTGGTCTT CCCTTTCGTC AAGGTCTTTG GCTATGTGAT GGAGGGTCTA
    AAATCGAAGA ATGCCCGTCA GCGTACTGAG TGCCTTGACG AGTTAACCTT CCTAATTGAA
    AGCTATGGCA TGAACATCTG TCCCCAATCG GCGGTGCGCG AGATTGCTCG CCAGATTTCC
    GACCGGGATA ATTCCGTACG CAACGCAGCA CTCAACTGCA TCGTCCAGGT CTTCTTTTTG
    TCCGGGGAAA AGACTTACAA GATGATTGGC CATCTTAACG AAAAGGACCT CTCAATGCTA
    GATGAGCGCA TCAAGCGAGC CAAAAAGACT AAGAAGCCCA CGCCACCGCC ATCTGTTGAT
    GTGCCGGCAC CTCAACGACA TGACAGCATC GAAATCGAAG ATGCTGAAGT GGGCAACGGT
    TGCGACGAGC TTCCACCACC AGATGAGGAC GGAACTTTCG ACCAGGCACC TTCGTCACAG
    CTGCTTCTAC TCCAGCAACA GCTTCAACAG TTACAACAGC AGGCCCAGCA ACAGAAGCCC
    AGCGGACCAT TCGGCTTGGA CTCGCAGGTA ATCAGCGAGA TCGAAAAAGA TTGGGTCCGA
    GTGGACCAAA TGGAGCAGAA GCCGCTACTG AACGTAGATA TCTCATCGCT GGACGAGCCT
    ATTAAGGTGA GGCCCACGCG GGCCGGCATT CACTACCCGC AGGAGAAGTT CGATCGGCTG
    ATTTCTCGAC AACACTATAT GCAGCAGACT TTAACGACGT CCCCGTCATC CACGGCTGGA
    ATGACCAGTG GAGTCTCCCC GTATCGCAGT CCTATGCGCT TGCAGCACCA GCAGCCTCAA
    CAGCAGCTGG AGAACAATAT ACCAAATTTG GCGGATGTTT TGCCAAAGCA CGATCCGCAG
    CTGGTAAAAG TTATAAAAGG TGTAAGCAGC ACGGACACTC TAAAGGCGCG CGCGGCTATT
    AACGAGCTTG CCGCCATCAT CGAGGCTCCA GAGAAGCAGG CTGTGCTGCG CGACTATGAA
    GAAATATTTA TTCAGAATGT GCTTGCACAG TTTAAGAATC TCTCACAAAT ACCATCGGCT
    CAATCCGTAG TTGTTTACCA GCCGCTATTA TCCATTCTGT ATACATTTTT CCATGCCAAC
    ATTCTGGGCA AAACGCTGAG CGTGGCCTGC ATCAAAAATC TAATGTCGGC GCTGTTGAAC
    CTTATGGCTG ATCCGAAGTT GGCCGTGGGT GATGATAGTC AGTATAACAA GGTTATTAAT
    GGCATATGTC TAAAAGTGTT GGACAAGGTA GATTTTACGA ATTTAAACTG TGCTTTGATA
    CGCCTATTAC GGGAGACTTG TCCGGAGGCA AAGCTGCCGA AGTTTACAGA CCTGCTGATG
    AAGTGCATAT GGCGCAACGT TAAGATGCTA CCCGAACGAA GCAACGAGCT GAACTATGAC
    GCCGTAATTC TCGAGGTGCA CGAGTTCATG CTCGCGCTGC CTAGCACTTG GTGGCAAAAC
    CGACCGTCGG ACACTCCAAT GCGCACGATT AAGACCATTC TGCATAACAT GGCAAAGGTG
    AAGGGGAATG CTATTCTGCA GCATCTCAAC CAGATTCCAA CACACTCGGA GCTACATACT
    TATTTGATAC GCATTCTCAA GAATTTCCAA AAGGATGGCT CTGCTTCAGG AATTGGTGCC
    TCCCCCCAGA GGGCCAAGGA GATTGCCTCC AAGCGAATTT CGCACCAAAC ACATGATACA
    GTATCACAGA TCTTTAAGCT GATCTCAGAT AGAGATACCA AACAGCAAGG TCTGCAGAAA
    CTTTACGACT TTAAGCAACA AAATCCTGAT ATCGATCTAA GTACCTTTCT GCAAGGCTCC
    AGTGCCCCAT TTCACAAGTA CATCGAGGAG GGTTTAGCCG AGATTGAGAG GAATCAGAAC
    GCTGGATCCA CACAGGATAA TCGGACGGCA GCTACTCGTT CTTATCTCAC AGATGTCAAC
    TACCAGAACA ACGGTCCCGA TCCCGATTTC TGGATGGACC GCCTGCAGTA TCATATGACC
    GGAGGGGCCG CCAAGCTAGC ATCTGCACGT TCAGCAGACG ATGGATCCCA TATGCTGGAC
    AATAAGGTGG TTGACGAGAA CCTCTGCCTG AACGGAATGA ACGCGCAAAA GGCGTCGCTC
    ATTAAGCGAG AAAAGCGGGA CATGTCACCA AATCGCTTGC AGCATCTCCA GGCAAAGCTG
    GCTCAAATCA AGAAAGAGAA TCATGCCCAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.