RpII140 cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following RpII140 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RpII140 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa04837 NM_057358.4
Latest version!
Drosophila melanogaster RNA polymerase II 140kD subunit (RpII140), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OFa04837 NM_001300394.1
Latest version!
Drosophila melanogaster RNA polymerase II 140kD subunit (RpII140), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa04837
    Clone ID Related Accession (Same CDS sequence) NM_057358.4 , NM_001300394.1
    Accession Version NM_057358.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product RNA polymerase II 140kD subunit, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_057358.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGTACG ACAACGAGGA AGAACTGTAC GAGGAGGAGA ATGCCGAGGA GATCTCTCAC 
    GAGCTGTGGC AGGAAGCCTG CTGGATCGTG ATTAACGCGT ACTTCGACGA GAAGGGCTTG
    GTGCGGCAGC AGCTGGACAG TTTCGATGAG TTCATTCAGA TGTCGGTGCA GCGCATTGTG
    GAGGATTCCC CCGCCATTGA GTTGCAGGCG GAAGCGCAGC ACACATCCGG AGAGGTGGAA
    ACCCCGCCCA GATTCTCGCT GAAATTCGAG CAAATCTATC TTTCCAAGCC AACACATTGG
    GAAAAGGATG GTTCGCCCAG TCCTATGATG CCGAACGAGG CCCGCCTGCG AAATCTAACT
    TACTCCGCTC CTCTCTACGT GGACATTACG AAAACCAAGA ATGTCGAGGG CCTGGATCCC
    GTGGAAACGC AGCATCAAAA GACTTTCATA GGCAAGATTC CCATCATGTT GAGATCAACG
    TACTGTTTGC TATCACAGCT TACTGATCGT GATCTTACGG AGCTGAACGA GTGCCCGTTG
    GATCCCGGTG GCTACTTCAT CATCAACGGC TCCGAGAAGG TGCTAATTGC CCAAGAGAAA
    ATGGCCACCA ACACAGTGTA CGTGTTCAGC ATGAAAGATG GCAAGTATGC CTTTAAGACT
    GAGATTCGGT CGTGTTTGGA GCACAGTTCC AGACCCACGT CTACACTGTG GGTAAACATG
    ATGGCAAGAG GATCGCAAAA CATAAAAAAG TCAGCCATTG GTCAGAGGAT TATTGCCATC
    TTACCGTACA TCAAACAGGA GATACCCATT ATGATTGTGT TTCGAGCATT GGGCTTCGTG
    GCCGATCGCG ATATTCTGGA GCATATCATC TATGACTTCG ACGATCCCGA GATGATGGAA
    ATGGTGAAAC CTTCTCTGGA CGAGGCTTTT GTAGTCCAAG AGCAGAATGT TGCCTTGAAT
    TTCATTGGAG CTAGAGGAGC ACGTCCCGGA GTCACAAAAG ACAAGCGTAT AAAGTACGCT
    AAGGAAATTT TGCAAAAGGA AATGCTGCCC CACGTTGGAG TATCCGATTT CTGCGAGACC
    AAGAAGGCAT ACTTTTTGGG CTACATGGTT CACCGGCTGC TCTTAGCCTC TTTGGGCAGG
    AGAGAACTCG ATGATCGAGA TCACTATGGA AACAAGCGAC TTGATCTCGC TGGACCCCTC
    CTGGCCTTCC TCTTCCGTGG TCTGTTCAAA AACCTGATGA AAGAGGTGCG CATGTACACA
    CAGAAGTTCA TCGATCGCGG AAAGGACTTC AACCTTGAAC TGGCCATCAA GACTAATATT
    ATTACTGATG GTTTAAGGTA TTCCTTGGCC ACTGGCAATT GGGGCGACCA GAAGAAGGCG
    CATCAGGCGA GAGCCGGTGT CTCCCAGGTG TTGAATCGCT TGACCTTCGC CTCTACTCTT
    TCCCATTTGC GGCGTGTGAA TTCGCCAATT GGCAGAGACG GTAAACTGGC CAAACCTCGT
    CAGTTGCACA ATACTCTTTG GGGTATGTTG TGTCCCGCTG AAACTCCTGA GGGAGCAGCT
    GTTGGTCTGG TGAAGAACCT GGCGCTCATG GCTTACATTT CTGTGGGATC ACAACCTTCT
    CCCATTCTGG AGTTCTTGGA GGAGTGGTCT ATGGAAAACC TGGAGGAAAT TGCTCCTTCT
    GCCATCGCTG ATGCTACAAA GATCTTTGTG AACGGCTGCT GGGTGGGCAT ACATCGTGAT
    CCTGAGCAGC TCATGGCAAC GCTTCGTAAG TTGCGTCGCC AGATGGATAT CATCGTGTCA
    GAGGTGTCTA TGATTCGAGA CATTCGAGAT CGTGAGATCC GAATCTATAC AGATGCTGGT
    CGCATTTGTC GGCCACTTCT CATCGTGGAA AACGGATCGC TGCTGCTTAA AAAGACTCAC
    GTGGAGATGT TAAAGGAGCG AGACTACAAC AACTACAGTT GGCAGGTCCT TGTAGCCTCT
    GGAGTGGTGG AGTACATTGA TACCCTTGAG GAGGAAACGG TCATGATCGC TATGTCTCCC
    TACGATCTGA AGCAGGACAA GGATTATGCT TATTGTACCA CATACACACA TTGTGAGATC
    CATCCAGCCA TGATCCTGGG CGTGTGCGCG TCCATTATTC CCTTCCCCGA TCACAATCAG
    AGTCCGCGTA ACACCTATCA AAGCGCTATG GGTAAGCAAG CTATGGGCGT TTATATTACC
    AACTTCCACG TGCGTATGGA CACACTAGCC CACGTACTTT ACTATCCGAT GAAGCCACTT
    GTGACCACAC GTTCCATGGA GTACCTGCGT TTCCGAGAAC TACCAGCTGG CATCAATTCA
    ATCGTGGCTA TTCTCTGCTA CACTGGCTAC AACCAGGAGG ATTCTGTCAT TCTGAATGCA
    TCCGCTGTGG AGCGTGGATT CTTCCGCTCT GTGTTCTACC GATCATACAA GGATTCGGAG
    AACAAACGTG TCGGTGATCA GGAAGAGAAC TTCGAGAAGC CTCATCGCGG CACCTGCCAA
    GGAATGAGAA ATGCTCACTA CGACAAGTTA GACGATGATG GAATTATTGC CCCAGGAATT
    CGTGTATCTG GCGACGATGT GGTTATTGGC AAGACCATTA CCCTTCCTGA AAACGACGAC
    GAACTGGACA GCAACACGAA GCGTTTCTCC AAACGAGATG CCTCCACTTT CCTGCGAAAC
    TCTGAGACGG GTATTGTGGA CCAGGTGATG CTTACGCTCA ACAGCGAGGG CTACAAGTTC
    TGCAAGATTC GAGTGCGCTC GGTGCGTATT CCGCAGATCG GCGATAAGTT CGCTTCTCGT
    CACGGACAAA AGGGAACGTG CGGCATCCAG TACCGTCAGG AGGACATGGC CTTCACATGC
    GAGGGATTGG CACCCGATAT CATTATTAAC CCCCACGCTA TCCCATCTCG TATGACAATT
    GGTCACTTGA TCGAGTGTCT GCAGGGAAAA CTGGGATCCA ACAAGGGAGA GATCGGTGAT
    GCTACACCCT TCAACGATGC CGTCAACGTG CAAAAGATCT CCACCTTCCT TCAGGAGTAC
    GGCTATCATC TTCGAGGCAA TGAGGTCATG TACAATGGCC ACACTGGACG AAAGATTAAT
    GCTCAGGTCT TCCTGGGGCC CACCTACTAT CAGCGTCTTA AGCACATGGT GGACGACAAG
    ATTCACTCTC GTGCTCGAGG CCCCGTCCAG ATCCTGGTGC GTCAGCCTAT GGAAGGTCGT
    GCTCGTGATG GTGGCTTGCG TTTCGGTGAG ATGGAGCGTG ATTGCCAGAT CTCCCACGGC
    GCCGCTCAGT TCCTGCGAGA ACGTTTATTC GAGGTCTCCG ATCCGTACCG CGTACACATC
    TGCAACTTCT GTGGCCTGAT TGCCATCGCC AATCTGCGCA ACAATACGTT CGAGTGCAAG
    GGCTGCAAGA ACAAGACGCA GATTTCCCAG GTGCGACTCC CATATGCGGC CAAGCTTCTC
    TTCCAGGAGC TGATGTCAAT GAACATTGCG CCGCGCCTGA TGGTCACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_476706.1
    CDS157..3687
    Translation

    Target ORF information:

    RefSeq Version NM_057358.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster RNA polymerase II 140kD subunit (RpII140), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_057358.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGTACG ACAACGAGGA AGAACTGTAC GAGGAGGAGA ATGCCGAGGA GATCTCTCAC 
    GAGCTGTGGC AGGAAGCCTG CTGGATCGTG ATTAACGCGT ACTTCGACGA GAAGGGCTTG
    GTGCGGCAGC AGCTGGACAG TTTCGATGAG TTCATTCAGA TGTCGGTGCA GCGCATTGTG
    GAGGATTCCC CCGCCATTGA GTTGCAGGCG GAAGCGCAGC ACACATCCGG AGAGGTGGAA
    ACCCCGCCCA GATTCTCGCT GAAATTCGAG CAAATCTATC TTTCCAAGCC AACACATTGG
    GAAAAGGATG GTTCGCCCAG TCCTATGATG CCGAACGAGG CCCGCCTGCG AAATCTAACT
    TACTCCGCTC CTCTCTACGT GGACATTACG AAAACCAAGA ATGTCGAGGG CCTGGATCCC
    GTGGAAACGC AGCATCAAAA GACTTTCATA GGCAAGATTC CCATCATGTT GAGATCAACG
    TACTGTTTGC TATCACAGCT TACTGATCGT GATCTTACGG AGCTGAACGA GTGCCCGTTG
    GATCCCGGTG GCTACTTCAT CATCAACGGC TCCGAGAAGG TGCTAATTGC CCAAGAGAAA
    ATGGCCACCA ACACAGTGTA CGTGTTCAGC ATGAAAGATG GCAAGTATGC CTTTAAGACT
    GAGATTCGGT CGTGTTTGGA GCACAGTTCC AGACCCACGT CTACACTGTG GGTAAACATG
    ATGGCAAGAG GATCGCAAAA CATAAAAAAG TCAGCCATTG GTCAGAGGAT TATTGCCATC
    TTACCGTACA TCAAACAGGA GATACCCATT ATGATTGTGT TTCGAGCATT GGGCTTCGTG
    GCCGATCGCG ATATTCTGGA GCATATCATC TATGACTTCG ACGATCCCGA GATGATGGAA
    ATGGTGAAAC CTTCTCTGGA CGAGGCTTTT GTAGTCCAAG AGCAGAATGT TGCCTTGAAT
    TTCATTGGAG CTAGAGGAGC ACGTCCCGGA GTCACAAAAG ACAAGCGTAT AAAGTACGCT
    AAGGAAATTT TGCAAAAGGA AATGCTGCCC CACGTTGGAG TATCCGATTT CTGCGAGACC
    AAGAAGGCAT ACTTTTTGGG CTACATGGTT CACCGGCTGC TCTTAGCCTC TTTGGGCAGG
    AGAGAACTCG ATGATCGAGA TCACTATGGA AACAAGCGAC TTGATCTCGC TGGACCCCTC
    CTGGCCTTCC TCTTCCGTGG TCTGTTCAAA AACCTGATGA AAGAGGTGCG CATGTACACA
    CAGAAGTTCA TCGATCGCGG AAAGGACTTC AACCTTGAAC TGGCCATCAA GACTAATATT
    ATTACTGATG GTTTAAGGTA TTCCTTGGCC ACTGGCAATT GGGGCGACCA GAAGAAGGCG
    CATCAGGCGA GAGCCGGTGT CTCCCAGGTG TTGAATCGCT TGACCTTCGC CTCTACTCTT
    TCCCATTTGC GGCGTGTGAA TTCGCCAATT GGCAGAGACG GTAAACTGGC CAAACCTCGT
    CAGTTGCACA ATACTCTTTG GGGTATGTTG TGTCCCGCTG AAACTCCTGA GGGAGCAGCT
    GTTGGTCTGG TGAAGAACCT GGCGCTCATG GCTTACATTT CTGTGGGATC ACAACCTTCT
    CCCATTCTGG AGTTCTTGGA GGAGTGGTCT ATGGAAAACC TGGAGGAAAT TGCTCCTTCT
    GCCATCGCTG ATGCTACAAA GATCTTTGTG AACGGCTGCT GGGTGGGCAT ACATCGTGAT
    CCTGAGCAGC TCATGGCAAC GCTTCGTAAG TTGCGTCGCC AGATGGATAT CATCGTGTCA
    GAGGTGTCTA TGATTCGAGA CATTCGAGAT CGTGAGATCC GAATCTATAC AGATGCTGGT
    CGCATTTGTC GGCCACTTCT CATCGTGGAA AACGGATCGC TGCTGCTTAA AAAGACTCAC
    GTGGAGATGT TAAAGGAGCG AGACTACAAC AACTACAGTT GGCAGGTCCT TGTAGCCTCT
    GGAGTGGTGG AGTACATTGA TACCCTTGAG GAGGAAACGG TCATGATCGC TATGTCTCCC
    TACGATCTGA AGCAGGACAA GGATTATGCT TATTGTACCA CATACACACA TTGTGAGATC
    CATCCAGCCA TGATCCTGGG CGTGTGCGCG TCCATTATTC CCTTCCCCGA TCACAATCAG
    AGTCCGCGTA ACACCTATCA AAGCGCTATG GGTAAGCAAG CTATGGGCGT TTATATTACC
    AACTTCCACG TGCGTATGGA CACACTAGCC CACGTACTTT ACTATCCGAT GAAGCCACTT
    GTGACCACAC GTTCCATGGA GTACCTGCGT TTCCGAGAAC TACCAGCTGG CATCAATTCA
    ATCGTGGCTA TTCTCTGCTA CACTGGCTAC AACCAGGAGG ATTCTGTCAT TCTGAATGCA
    TCCGCTGTGG AGCGTGGATT CTTCCGCTCT GTGTTCTACC GATCATACAA GGATTCGGAG
    AACAAACGTG TCGGTGATCA GGAAGAGAAC TTCGAGAAGC CTCATCGCGG CACCTGCCAA
    GGAATGAGAA ATGCTCACTA CGACAAGTTA GACGATGATG GAATTATTGC CCCAGGAATT
    CGTGTATCTG GCGACGATGT GGTTATTGGC AAGACCATTA CCCTTCCTGA AAACGACGAC
    GAACTGGACA GCAACACGAA GCGTTTCTCC AAACGAGATG CCTCCACTTT CCTGCGAAAC
    TCTGAGACGG GTATTGTGGA CCAGGTGATG CTTACGCTCA ACAGCGAGGG CTACAAGTTC
    TGCAAGATTC GAGTGCGCTC GGTGCGTATT CCGCAGATCG GCGATAAGTT CGCTTCTCGT
    CACGGACAAA AGGGAACGTG CGGCATCCAG TACCGTCAGG AGGACATGGC CTTCACATGC
    GAGGGATTGG CACCCGATAT CATTATTAAC CCCCACGCTA TCCCATCTCG TATGACAATT
    GGTCACTTGA TCGAGTGTCT GCAGGGAAAA CTGGGATCCA ACAAGGGAGA GATCGGTGAT
    GCTACACCCT TCAACGATGC CGTCAACGTG CAAAAGATCT CCACCTTCCT TCAGGAGTAC
    GGCTATCATC TTCGAGGCAA TGAGGTCATG TACAATGGCC ACACTGGACG AAAGATTAAT
    GCTCAGGTCT TCCTGGGGCC CACCTACTAT CAGCGTCTTA AGCACATGGT GGACGACAAG
    ATTCACTCTC GTGCTCGAGG CCCCGTCCAG ATCCTGGTGC GTCAGCCTAT GGAAGGTCGT
    GCTCGTGATG GTGGCTTGCG TTTCGGTGAG ATGGAGCGTG ATTGCCAGAT CTCCCACGGC
    GCCGCTCAGT TCCTGCGAGA ACGTTTATTC GAGGTCTCCG ATCCGTACCG CGTACACATC
    TGCAACTTCT GTGGCCTGAT TGCCATCGCC AATCTGCGCA ACAATACGTT CGAGTGCAAG
    GGCTGCAAGA ACAAGACGCA GATTTCCCAG GTGCGACTCC CATATGCGGC CAAGCTTCTC
    TTCCAGGAGC TGATGTCAAT GAACATTGCG CCGCGCCTGA TGGTCACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa04837
    Clone ID Related Accession (Same CDS sequence) NM_057358.4 , NM_001300394.1
    Accession Version NM_001300394.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3531bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product RNA polymerase II 140kD subunit, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGTACG ACAACGAGGA AGAACTGTAC GAGGAGGAGA ATGCCGAGGA GATCTCTCAC 
    GAGCTGTGGC AGGAAGCCTG CTGGATCGTG ATTAACGCGT ACTTCGACGA GAAGGGCTTG
    GTGCGGCAGC AGCTGGACAG TTTCGATGAG TTCATTCAGA TGTCGGTGCA GCGCATTGTG
    GAGGATTCCC CCGCCATTGA GTTGCAGGCG GAAGCGCAGC ACACATCCGG AGAGGTGGAA
    ACCCCGCCCA GATTCTCGCT GAAATTCGAG CAAATCTATC TTTCCAAGCC AACACATTGG
    GAAAAGGATG GTTCGCCCAG TCCTATGATG CCGAACGAGG CCCGCCTGCG AAATCTAACT
    TACTCCGCTC CTCTCTACGT GGACATTACG AAAACCAAGA ATGTCGAGGG CCTGGATCCC
    GTGGAAACGC AGCATCAAAA GACTTTCATA GGCAAGATTC CCATCATGTT GAGATCAACG
    TACTGTTTGC TATCACAGCT TACTGATCGT GATCTTACGG AGCTGAACGA GTGCCCGTTG
    GATCCCGGTG GCTACTTCAT CATCAACGGC TCCGAGAAGG TGCTAATTGC CCAAGAGAAA
    ATGGCCACCA ACACAGTGTA CGTGTTCAGC ATGAAAGATG GCAAGTATGC CTTTAAGACT
    GAGATTCGGT CGTGTTTGGA GCACAGTTCC AGACCCACGT CTACACTGTG GGTAAACATG
    ATGGCAAGAG GATCGCAAAA CATAAAAAAG TCAGCCATTG GTCAGAGGAT TATTGCCATC
    TTACCGTACA TCAAACAGGA GATACCCATT ATGATTGTGT TTCGAGCATT GGGCTTCGTG
    GCCGATCGCG ATATTCTGGA GCATATCATC TATGACTTCG ACGATCCCGA GATGATGGAA
    ATGGTGAAAC CTTCTCTGGA CGAGGCTTTT GTAGTCCAAG AGCAGAATGT TGCCTTGAAT
    TTCATTGGAG CTAGAGGAGC ACGTCCCGGA GTCACAAAAG ACAAGCGTAT AAAGTACGCT
    AAGGAAATTT TGCAAAAGGA AATGCTGCCC CACGTTGGAG TATCCGATTT CTGCGAGACC
    AAGAAGGCAT ACTTTTTGGG CTACATGGTT CACCGGCTGC TCTTAGCCTC TTTGGGCAGG
    AGAGAACTCG ATGATCGAGA TCACTATGGA AACAAGCGAC TTGATCTCGC TGGACCCCTC
    CTGGCCTTCC TCTTCCGTGG TCTGTTCAAA AACCTGATGA AAGAGGTGCG CATGTACACA
    CAGAAGTTCA TCGATCGCGG AAAGGACTTC AACCTTGAAC TGGCCATCAA GACTAATATT
    ATTACTGATG GTTTAAGGTA TTCCTTGGCC ACTGGCAATT GGGGCGACCA GAAGAAGGCG
    CATCAGGCGA GAGCCGGTGT CTCCCAGGTG TTGAATCGCT TGACCTTCGC CTCTACTCTT
    TCCCATTTGC GGCGTGTGAA TTCGCCAATT GGCAGAGACG GTAAACTGGC CAAACCTCGT
    CAGTTGCACA ATACTCTTTG GGGTATGTTG TGTCCCGCTG AAACTCCTGA GGGAGCAGCT
    GTTGGTCTGG TGAAGAACCT GGCGCTCATG GCTTACATTT CTGTGGGATC ACAACCTTCT
    CCCATTCTGG AGTTCTTGGA GGAGTGGTCT ATGGAAAACC TGGAGGAAAT TGCTCCTTCT
    GCCATCGCTG ATGCTACAAA GATCTTTGTG AACGGCTGCT GGGTGGGCAT ACATCGTGAT
    CCTGAGCAGC TCATGGCAAC GCTTCGTAAG TTGCGTCGCC AGATGGATAT CATCGTGTCA
    GAGGTGTCTA TGATTCGAGA CATTCGAGAT CGTGAGATCC GAATCTATAC AGATGCTGGT
    CGCATTTGTC GGCCACTTCT CATCGTGGAA AACGGATCGC TGCTGCTTAA AAAGACTCAC
    GTGGAGATGT TAAAGGAGCG AGACTACAAC AACTACAGTT GGCAGGTCCT TGTAGCCTCT
    GGAGTGGTGG AGTACATTGA TACCCTTGAG GAGGAAACGG TCATGATCGC TATGTCTCCC
    TACGATCTGA AGCAGGACAA GGATTATGCT TATTGTACCA CATACACACA TTGTGAGATC
    CATCCAGCCA TGATCCTGGG CGTGTGCGCG TCCATTATTC CCTTCCCCGA TCACAATCAG
    AGTCCGCGTA ACACCTATCA AAGCGCTATG GGTAAGCAAG CTATGGGCGT TTATATTACC
    AACTTCCACG TGCGTATGGA CACACTAGCC CACGTACTTT ACTATCCGAT GAAGCCACTT
    GTGACCACAC GTTCCATGGA GTACCTGCGT TTCCGAGAAC TACCAGCTGG CATCAATTCA
    ATCGTGGCTA TTCTCTGCTA CACTGGCTAC AACCAGGAGG ATTCTGTCAT TCTGAATGCA
    TCCGCTGTGG AGCGTGGATT CTTCCGCTCT GTGTTCTACC GATCATACAA GGATTCGGAG
    AACAAACGTG TCGGTGATCA GGAAGAGAAC TTCGAGAAGC CTCATCGCGG CACCTGCCAA
    GGAATGAGAA ATGCTCACTA CGACAAGTTA GACGATGATG GAATTATTGC CCCAGGAATT
    CGTGTATCTG GCGACGATGT GGTTATTGGC AAGACCATTA CCCTTCCTGA AAACGACGAC
    GAACTGGACA GCAACACGAA GCGTTTCTCC AAACGAGATG CCTCCACTTT CCTGCGAAAC
    TCTGAGACGG GTATTGTGGA CCAGGTGATG CTTACGCTCA ACAGCGAGGG CTACAAGTTC
    TGCAAGATTC GAGTGCGCTC GGTGCGTATT CCGCAGATCG GCGATAAGTT CGCTTCTCGT
    CACGGACAAA AGGGAACGTG CGGCATCCAG TACCGTCAGG AGGACATGGC CTTCACATGC
    GAGGGATTGG CACCCGATAT CATTATTAAC CCCCACGCTA TCCCATCTCG TATGACAATT
    GGTCACTTGA TCGAGTGTCT GCAGGGAAAA CTGGGATCCA ACAAGGGAGA GATCGGTGAT
    GCTACACCCT TCAACGATGC CGTCAACGTG CAAAAGATCT CCACCTTCCT TCAGGAGTAC
    GGCTATCATC TTCGAGGCAA TGAGGTCATG TACAATGGCC ACACTGGACG AAAGATTAAT
    GCTCAGGTCT TCCTGGGGCC CACCTACTAT CAGCGTCTTA AGCACATGGT GGACGACAAG
    ATTCACTCTC GTGCTCGAGG CCCCGTCCAG ATCCTGGTGC GTCAGCCTAT GGAAGGTCGT
    GCTCGTGATG GTGGCTTGCG TTTCGGTGAG ATGGAGCGTG ATTGCCAGAT CTCCCACGGC
    GCCGCTCAGT TCCTGCGAGA ACGTTTATTC GAGGTCTCCG ATCCGTACCG CGTACACATC
    TGCAACTTCT GTGGCCTGAT TGCCATCGCC AATCTGCGCA ACAATACGTT CGAGTGCAAG
    GGCTGCAAGA ACAAGACGCA GATTTCCCAG GTGCGACTCC CATATGCGGC CAAGCTTCTC
    TTCCAGGAGC TGATGTCAAT GAACATTGCG CCGCGCCTGA TGGTCACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001287323.1
    CDS157..3687
    Translation

    Target ORF information:

    RefSeq Version NM_001300394.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster RNA polymerase II 140kD subunit (RpII140), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001300394.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGATGTACG ACAACGAGGA AGAACTGTAC GAGGAGGAGA ATGCCGAGGA GATCTCTCAC 
    GAGCTGTGGC AGGAAGCCTG CTGGATCGTG ATTAACGCGT ACTTCGACGA GAAGGGCTTG
    GTGCGGCAGC AGCTGGACAG TTTCGATGAG TTCATTCAGA TGTCGGTGCA GCGCATTGTG
    GAGGATTCCC CCGCCATTGA GTTGCAGGCG GAAGCGCAGC ACACATCCGG AGAGGTGGAA
    ACCCCGCCCA GATTCTCGCT GAAATTCGAG CAAATCTATC TTTCCAAGCC AACACATTGG
    GAAAAGGATG GTTCGCCCAG TCCTATGATG CCGAACGAGG CCCGCCTGCG AAATCTAACT
    TACTCCGCTC CTCTCTACGT GGACATTACG AAAACCAAGA ATGTCGAGGG CCTGGATCCC
    GTGGAAACGC AGCATCAAAA GACTTTCATA GGCAAGATTC CCATCATGTT GAGATCAACG
    TACTGTTTGC TATCACAGCT TACTGATCGT GATCTTACGG AGCTGAACGA GTGCCCGTTG
    GATCCCGGTG GCTACTTCAT CATCAACGGC TCCGAGAAGG TGCTAATTGC CCAAGAGAAA
    ATGGCCACCA ACACAGTGTA CGTGTTCAGC ATGAAAGATG GCAAGTATGC CTTTAAGACT
    GAGATTCGGT CGTGTTTGGA GCACAGTTCC AGACCCACGT CTACACTGTG GGTAAACATG
    ATGGCAAGAG GATCGCAAAA CATAAAAAAG TCAGCCATTG GTCAGAGGAT TATTGCCATC
    TTACCGTACA TCAAACAGGA GATACCCATT ATGATTGTGT TTCGAGCATT GGGCTTCGTG
    GCCGATCGCG ATATTCTGGA GCATATCATC TATGACTTCG ACGATCCCGA GATGATGGAA
    ATGGTGAAAC CTTCTCTGGA CGAGGCTTTT GTAGTCCAAG AGCAGAATGT TGCCTTGAAT
    TTCATTGGAG CTAGAGGAGC ACGTCCCGGA GTCACAAAAG ACAAGCGTAT AAAGTACGCT
    AAGGAAATTT TGCAAAAGGA AATGCTGCCC CACGTTGGAG TATCCGATTT CTGCGAGACC
    AAGAAGGCAT ACTTTTTGGG CTACATGGTT CACCGGCTGC TCTTAGCCTC TTTGGGCAGG
    AGAGAACTCG ATGATCGAGA TCACTATGGA AACAAGCGAC TTGATCTCGC TGGACCCCTC
    CTGGCCTTCC TCTTCCGTGG TCTGTTCAAA AACCTGATGA AAGAGGTGCG CATGTACACA
    CAGAAGTTCA TCGATCGCGG AAAGGACTTC AACCTTGAAC TGGCCATCAA GACTAATATT
    ATTACTGATG GTTTAAGGTA TTCCTTGGCC ACTGGCAATT GGGGCGACCA GAAGAAGGCG
    CATCAGGCGA GAGCCGGTGT CTCCCAGGTG TTGAATCGCT TGACCTTCGC CTCTACTCTT
    TCCCATTTGC GGCGTGTGAA TTCGCCAATT GGCAGAGACG GTAAACTGGC CAAACCTCGT
    CAGTTGCACA ATACTCTTTG GGGTATGTTG TGTCCCGCTG AAACTCCTGA GGGAGCAGCT
    GTTGGTCTGG TGAAGAACCT GGCGCTCATG GCTTACATTT CTGTGGGATC ACAACCTTCT
    CCCATTCTGG AGTTCTTGGA GGAGTGGTCT ATGGAAAACC TGGAGGAAAT TGCTCCTTCT
    GCCATCGCTG ATGCTACAAA GATCTTTGTG AACGGCTGCT GGGTGGGCAT ACATCGTGAT
    CCTGAGCAGC TCATGGCAAC GCTTCGTAAG TTGCGTCGCC AGATGGATAT CATCGTGTCA
    GAGGTGTCTA TGATTCGAGA CATTCGAGAT CGTGAGATCC GAATCTATAC AGATGCTGGT
    CGCATTTGTC GGCCACTTCT CATCGTGGAA AACGGATCGC TGCTGCTTAA AAAGACTCAC
    GTGGAGATGT TAAAGGAGCG AGACTACAAC AACTACAGTT GGCAGGTCCT TGTAGCCTCT
    GGAGTGGTGG AGTACATTGA TACCCTTGAG GAGGAAACGG TCATGATCGC TATGTCTCCC
    TACGATCTGA AGCAGGACAA GGATTATGCT TATTGTACCA CATACACACA TTGTGAGATC
    CATCCAGCCA TGATCCTGGG CGTGTGCGCG TCCATTATTC CCTTCCCCGA TCACAATCAG
    AGTCCGCGTA ACACCTATCA AAGCGCTATG GGTAAGCAAG CTATGGGCGT TTATATTACC
    AACTTCCACG TGCGTATGGA CACACTAGCC CACGTACTTT ACTATCCGAT GAAGCCACTT
    GTGACCACAC GTTCCATGGA GTACCTGCGT TTCCGAGAAC TACCAGCTGG CATCAATTCA
    ATCGTGGCTA TTCTCTGCTA CACTGGCTAC AACCAGGAGG ATTCTGTCAT TCTGAATGCA
    TCCGCTGTGG AGCGTGGATT CTTCCGCTCT GTGTTCTACC GATCATACAA GGATTCGGAG
    AACAAACGTG TCGGTGATCA GGAAGAGAAC TTCGAGAAGC CTCATCGCGG CACCTGCCAA
    GGAATGAGAA ATGCTCACTA CGACAAGTTA GACGATGATG GAATTATTGC CCCAGGAATT
    CGTGTATCTG GCGACGATGT GGTTATTGGC AAGACCATTA CCCTTCCTGA AAACGACGAC
    GAACTGGACA GCAACACGAA GCGTTTCTCC AAACGAGATG CCTCCACTTT CCTGCGAAAC
    TCTGAGACGG GTATTGTGGA CCAGGTGATG CTTACGCTCA ACAGCGAGGG CTACAAGTTC
    TGCAAGATTC GAGTGCGCTC GGTGCGTATT CCGCAGATCG GCGATAAGTT CGCTTCTCGT
    CACGGACAAA AGGGAACGTG CGGCATCCAG TACCGTCAGG AGGACATGGC CTTCACATGC
    GAGGGATTGG CACCCGATAT CATTATTAAC CCCCACGCTA TCCCATCTCG TATGACAATT
    GGTCACTTGA TCGAGTGTCT GCAGGGAAAA CTGGGATCCA ACAAGGGAGA GATCGGTGAT
    GCTACACCCT TCAACGATGC CGTCAACGTG CAAAAGATCT CCACCTTCCT TCAGGAGTAC
    GGCTATCATC TTCGAGGCAA TGAGGTCATG TACAATGGCC ACACTGGACG AAAGATTAAT
    GCTCAGGTCT TCCTGGGGCC CACCTACTAT CAGCGTCTTA AGCACATGGT GGACGACAAG
    ATTCACTCTC GTGCTCGAGG CCCCGTCCAG ATCCTGGTGC GTCAGCCTAT GGAAGGTCGT
    GCTCGTGATG GTGGCTTGCG TTTCGGTGAG ATGGAGCGTG ATTGCCAGAT CTCCCACGGC
    GCCGCTCAGT TCCTGCGAGA ACGTTTATTC GAGGTCTCCG ATCCGTACCG CGTACACATC
    TGCAACTTCT GTGGCCTGAT TGCCATCGCC AATCTGCGCA ACAATACGTT CGAGTGCAAG
    GGCTGCAAGA ACAAGACGCA GATTTCCCAG GTGCGACTCC CATATGCGGC CAAGCTTCTC
    TTCCAGGAGC TGATGTCAAT GAACATTGCG CCGCGCCTGA TGGTCACCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.