CG8774 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG8774
Entrez Gene ID	41635
Full Name	CG8774 gene product from transcript CG8774-RB
Synonyms	Dmel\CG8774
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 87E4-87E5

mRNA and Protein(s)

mRNA	Protein	Name
NM_169502.2	NP_731785.1	uncharacterized protein, isoform B
NM_142017.3	NP_650274.2	uncharacterized protein, isoform A

Pathways from BioSystems

Homologies

Homo sapiens (human)	ENPEP	NP_001968.3
	ENPEP	XP_001090456.1
Mus musculus (house mouse)	Enpep	NP_031960.1
Drosophila melanogaster (fruit fly)	CG32473	NP_731786.1
Gallus gallus (chicken)	ENPEP	XP_426327.3
Xenopus tropicalis (tropical clawed frog)	enpep	XP_002934295.1
Pan troglodytes (chimpanzee)	ENPEP	XP_517397.2
Canis lupus familiaris (dog)	ENPEP	XP_535696.2
Drosophila melanogaster (fruit fly)	CG8773	NP_650273.2
Drosophila melanogaster (fruit fly)	CG8774	NP_650274.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003077	XP_003436384.1
Bos taurus (cattle)	ENPEP	NP_001033116.1
Rattus norvegicus (Norway rat)	Enpep	NP_071587.2
Danio rerio (zebrafish)	enpep	NP_001186660.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG8774 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG8774 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04786	NM_169502.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG8774), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa04787	NM_142017.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG8774), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG8774 ( NM_169502.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731785.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG8774), mRNA.

Price & Availability↑

CloneID	OFa04786
Clone ID Related Accession (Same CDS sequence)	NM_169502.2
Accession Version	NM_169502.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169502.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCGTG AAGATACGAC GGATTACCGC CTGCCCACGA ATCTGGTGCC CACGCACTAC 
GAGCTCTACT GGCATCCGGA TCTGGAGACA GGAAACTTCA CCGGACAACA GAGGATCAGC 
ATCAAGGTGG TGGAGGCCAC TAATCAGATT ATTCTGCACT CATATCTCCT GGATATCACA 
AGTGTCTATG TGCTGAATAG AGAGGTGGAA AAGTTTGAGC TTGAGGAGGA ACGTCAGTTC 
CTGATCATAA CTTTGACGGA AGAGCTGGCG GTGGACGCCA GCATTACTCT CGGAATCATT 
TTTGGTGGAC AGATGAAGGA TAAGCTTGTG GGTCTCTACA GCAGTACATA TCTAAACGAG 
GCAGGAGCTA CCAGAACCAT TTCTACGACG AAATTCGAAC CCACTTACGC GCGTCAGGCC 
TTCCCATGCT TCGATGAACC AGCCATGAAG GCCACATTCG CCATTACAGT GGTTCATCCA 
TCGGGATCCT ACCACGCCGT TTCCAACATG CAACAAACGG AATCCAATTA TCTGGGTGAC 
TATACTGAGG CCATCTTCGA GACGAGTGTG TCGATGAGCA CCTACCTGGT ATGCATCATT 
GTGTCCGACT TCGCTTCCCA ATCGACCACG GTGAAGGCCA ACGGAATTGG CGAGGACTTC 
TCCATGCAAG CCTATGCCAC CTCGCATCAG ATCAACAAGG TCGAGTTTGC TCTGGAGTTC 
GGACAAGCTG TCACGGAGTA CTATATTCAG TACTATAAGG TGCCCTATCC GCTGACCAAA 
CTGGACATGG CCGCCATTCC CGACTTTGCA TCTGGGGCCA TGGAGCACTG GGGATTGGTG 
ACTTACAGAG AGACCGCTCT GCTGTACGAT CCCAGTTACA GTTCCACAGC GAACAAACAA 
TCGATAGCCG GAACACTGGC CCATGAGATT GCACACCAGT GGTTCGGAAA CCTGGTCACC 
ATGAAGTGGT GGAACGATCT GTGGCTGAAC GAGGGATTCG CCCGCTACAT GCAGTACAAG 
GGAGTCAATG CTGTGCACCC GGACTGGGGA ATGCTGGAGC AATTCCAAAT CGTGGCCCTG 
CAACCCGTGT TGTTATACGA TGCCAAGCTA TCATCCCATC CGATTGTCCA GAAGGTAGAA 
AGCCCCGATG AAATTACGGC CATATTCGAC ACCATCAGTT ACGAAAAGGG AGGATCCGTG 
ATCCGCATGC TGGAGACACT TGTGGGCGCC GAAAAGTTTG AGGAGGCGGT GACCAATTAC 
CTGGTCAAGC ATCAGTTCAA TAACACGGTG ACCGACGATT TCCTCACCGA AGTCGAAGCT 
GTGGTAACTG ACTTGGATAT CAAGAAACTG ATGCTCACCT GGACCGAACA GATGGGTTAT 
CCGGTGTTGA ATGTCTCCAA GGTTGCAGAT GGTAGTTTTA AGGTTACCCA GCAGCGATTC 
CTCTCCAATC CAGCCAGTTA CGAGGAAGCC CCTTCAGATA GCGCATACGG ATACAAATGG 
AGTGTTCCGA TTACCTGGTT TGCTGATGAT GGTTCAGAGA ACAGCTTCAT ATACGACTAC 
GATGTGGACT CTGTGGGTAT TGCCGTGCCC AGCGAGGTGC AGTGGATCAA GTTGAATGTC 
AACCAAACGG GCTACTATCG GGTTAACTAC GAGGAGAGCC TGTGGGCCCT GCTCATCCAG 
CAGCTAACCA CCAGTCCCGC TCGATTCGAA ATCGCCGATC GTGGCCATCT TTTGAACGAT 
GCCTTCGCCC TGGCGGATGC CAACCAACTA TCCTACAAAA TTCCTCTGGA TATGACCGCT 
TACTTGGCCC AGGAGCGCGA CTTCGTGCCC TGGTATGTGG CCTCCAATAA GCTGAGGTCG 
CTCCATCGCA GTCTCATGTT CAGCGAGGGA TACGTGTCGT ACCTCACCTA TGCCAGAAGT 
CTGATCGCTG GCGTATACGA GGAGGTGGGT TGGACTGTGG ACGCCGACAA TCACTTGAAG 
AACCGCCTGC GTGTTTCCAT TTTGACCGCC GCCTGTGCCC TGGGTGTTCC AGATTGTCTG 
CAGCAGGCTT CCGAGCGCTT CAGCACCTTC CTGGAGAATC CGACCACTCG ACCTTCCCCC 
GATCTTCGCG AGATTGTCTA CTACTACGGC ATGCAGCAGT CCACCAGCCA ATCGAGCTGG 
GATCAGTTGT TCCAACTCTT TGTGGCCGAA ACCGATGCCA GCGAGAAACT GAAGCTGATG 
TACGGATTGT CTGGCGTACG GAACAGCCAG TATCTGTTCG ACTTCCTGGC ACAGGCCTCC 
AGTGATGAGA GCATCGTTCG CTCCCAGGAC TATTTCACCT GTGTGCAGTA CATAGCCGCC 
AATCCTGTGG GTGAACCGGT GGTCTGGGAA TTCTACCGCG AACAGTGGCC CCAACTCACC 
ACCCGTTTTG GCCTGAACAA CCGCAACTTT GGACGACTTA TCGCCCAGAT TACGGCCAAC 
TTTGCCAGCT CCGTGAAACT GGAGGAGGTT CAGCACTTCT TCTCCAAGTA CCCCGAATCG 
GGAGCTGGAG CTAACTCCCG GTTGGAGGCC GTGGAAACCA TCAAGTACAA CATCGAATGG 
CTATCCAGAA ACGAAGCTGA CATCACCGAC TGGCTAAGTG GAACGGCCTC GCCGCTGACC 
AAGAAAAACC AATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731785.1
CDS	329..2986
Translation	MKREDTTDYRLPTNLVPTHYELYWHPDLETGNFTGQQRISIKVVEATNQIILHSYLLDITSVYVLNREVEKFELEEERQFLIITLTEELAVDASITLGIIFGGQMKDKLVGLYSSTYLNEAGATRTISTTKFEPTYARQAFPCFDEPAMKATFAITVVHPSGSYHAVSNMQQTESNYLGDYTEAIFETSVSMSTYLVCIIVSDFASQSTTVKANGIGEDFSMQAYATSHQINKVEFALEFGQAVTEYYIQYYKVPYPLTKLDMAAIPDFASGAMEHWGLVTYRETALLYDPSYSSTANKQSIAGTLAHEIAHQWFGNLVTMKWWNDLWLNEGFARYMQYKGVNAVHPDWGMLEQFQIVALQPVLLYDAKLSSHPIVQKVESPDEITAIFDTISYEKGGSVIRMLETLVGAEKFEEAVTNYLVKHQFNNTVTDDFLTEVEAVVTDLDIKKLMLTWTEQMGYPVLNVSKVADGSFKVTQQRFLSNPASYEEAPSDSAYGYKWSVPITWFADDGSENSFIYDYDVDSVGIAVPSEVQWIKLNVNQTGYYRVNYEESLWALLIQQLTTSPARFEIADRGHLLNDAFALADANQLSYKIPLDMTAYLAQERDFVPWYVASNKLRSLHRSLMFSEGYVSYLTYARSLIAGVYEEVGWTVDADNHLKNRLRVSILTAACALGVPDCLQQASERFSTFLENPTTRPSPDLREIVYYYGMQQSTSQSSWDQLFQLFVAETDASEKLKLMYGLSGVRNSQYLFDFLAQASSDESIVRSQDYFTCVQYIAANPVGEPVVWEFYREQWPQLTTRFGLNNRNFGRLIAQITANFASSVKLEEVQHFFSKYPESGAGANSRLEAVETIKYNIEWLSRNEADITDWLSGTASPLTKKNQL

Target ORF information:

RefSeq Version	NM_169502.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG8774), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169502.2

ORF Insert Sequence:

ATGAAGCGTG AAGATACGAC GGATTACCGC CTGCCCACGA ATCTGGTGCC CACGCACTAC 
GAGCTCTACT GGCATCCGGA TCTGGAGACA GGAAACTTCA CCGGACAACA GAGGATCAGC 
ATCAAGGTGG TGGAGGCCAC TAATCAGATT ATTCTGCACT CATATCTCCT GGATATCACA 
AGTGTCTATG TGCTGAATAG AGAGGTGGAA AAGTTTGAGC TTGAGGAGGA ACGTCAGTTC 
CTGATCATAA CTTTGACGGA AGAGCTGGCG GTGGACGCCA GCATTACTCT CGGAATCATT 
TTTGGTGGAC AGATGAAGGA TAAGCTTGTG GGTCTCTACA GCAGTACATA TCTAAACGAG 
GCAGGAGCTA CCAGAACCAT TTCTACGACG AAATTCGAAC CCACTTACGC GCGTCAGGCC 
TTCCCATGCT TCGATGAACC AGCCATGAAG GCCACATTCG CCATTACAGT GGTTCATCCA 
TCGGGATCCT ACCACGCCGT TTCCAACATG CAACAAACGG AATCCAATTA TCTGGGTGAC 
TATACTGAGG CCATCTTCGA GACGAGTGTG TCGATGAGCA CCTACCTGGT ATGCATCATT 
GTGTCCGACT TCGCTTCCCA ATCGACCACG GTGAAGGCCA ACGGAATTGG CGAGGACTTC 
TCCATGCAAG CCTATGCCAC CTCGCATCAG ATCAACAAGG TCGAGTTTGC TCTGGAGTTC 
GGACAAGCTG TCACGGAGTA CTATATTCAG TACTATAAGG TGCCCTATCC GCTGACCAAA 
CTGGACATGG CCGCCATTCC CGACTTTGCA TCTGGGGCCA TGGAGCACTG GGGATTGGTG 
ACTTACAGAG AGACCGCTCT GCTGTACGAT CCCAGTTACA GTTCCACAGC GAACAAACAA 
TCGATAGCCG GAACACTGGC CCATGAGATT GCACACCAGT GGTTCGGAAA CCTGGTCACC 
ATGAAGTGGT GGAACGATCT GTGGCTGAAC GAGGGATTCG CCCGCTACAT GCAGTACAAG 
GGAGTCAATG CTGTGCACCC GGACTGGGGA ATGCTGGAGC AATTCCAAAT CGTGGCCCTG 
CAACCCGTGT TGTTATACGA TGCCAAGCTA TCATCCCATC CGATTGTCCA GAAGGTAGAA 
AGCCCCGATG AAATTACGGC CATATTCGAC ACCATCAGTT ACGAAAAGGG AGGATCCGTG 
ATCCGCATGC TGGAGACACT TGTGGGCGCC GAAAAGTTTG AGGAGGCGGT GACCAATTAC 
CTGGTCAAGC ATCAGTTCAA TAACACGGTG ACCGACGATT TCCTCACCGA AGTCGAAGCT 
GTGGTAACTG ACTTGGATAT CAAGAAACTG ATGCTCACCT GGACCGAACA GATGGGTTAT 
CCGGTGTTGA ATGTCTCCAA GGTTGCAGAT GGTAGTTTTA AGGTTACCCA GCAGCGATTC 
CTCTCCAATC CAGCCAGTTA CGAGGAAGCC CCTTCAGATA GCGCATACGG ATACAAATGG 
AGTGTTCCGA TTACCTGGTT TGCTGATGAT GGTTCAGAGA ACAGCTTCAT ATACGACTAC 
GATGTGGACT CTGTGGGTAT TGCCGTGCCC AGCGAGGTGC AGTGGATCAA GTTGAATGTC 
AACCAAACGG GCTACTATCG GGTTAACTAC GAGGAGAGCC TGTGGGCCCT GCTCATCCAG 
CAGCTAACCA CCAGTCCCGC TCGATTCGAA ATCGCCGATC GTGGCCATCT TTTGAACGAT 
GCCTTCGCCC TGGCGGATGC CAACCAACTA TCCTACAAAA TTCCTCTGGA TATGACCGCT 
TACTTGGCCC AGGAGCGCGA CTTCGTGCCC TGGTATGTGG CCTCCAATAA GCTGAGGTCG 
CTCCATCGCA GTCTCATGTT CAGCGAGGGA TACGTGTCGT ACCTCACCTA TGCCAGAAGT 
CTGATCGCTG GCGTATACGA GGAGGTGGGT TGGACTGTGG ACGCCGACAA TCACTTGAAG 
AACCGCCTGC GTGTTTCCAT TTTGACCGCC GCCTGTGCCC TGGGTGTTCC AGATTGTCTG 
CAGCAGGCTT CCGAGCGCTT CAGCACCTTC CTGGAGAATC CGACCACTCG ACCTTCCCCC 
GATCTTCGCG AGATTGTCTA CTACTACGGC ATGCAGCAGT CCACCAGCCA ATCGAGCTGG 
GATCAGTTGT TCCAACTCTT TGTGGCCGAA ACCGATGCCA GCGAGAAACT GAAGCTGATG 
TACGGATTGT CTGGCGTACG GAACAGCCAG TATCTGTTCG ACTTCCTGGC ACAGGCCTCC 
AGTGATGAGA GCATCGTTCG CTCCCAGGAC TATTTCACCT GTGTGCAGTA CATAGCCGCC 
AATCCTGTGG GTGAACCGGT GGTCTGGGAA TTCTACCGCG AACAGTGGCC CCAACTCACC 
ACCCGTTTTG GCCTGAACAA CCGCAACTTT GGACGACTTA TCGCCCAGAT TACGGCCAAC 
TTTGCCAGCT CCGTGAAACT GGAGGAGGTT CAGCACTTCT TCTCCAAGTA CCCCGAATCG 
GGAGCTGGAG CTAACTCCCG GTTGGAGGCC GTGGAAACCA TCAAGTACAA CATCGAATGG 
CTATCCAGAA ACGAAGCTGA CATCACCGAC TGGCTAAGTG GAACGGCCTC GCCGCTGACC 
AAGAAAAACC AATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG8774 ( NM_142017.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650274.2 Drosophila melanogaster uncharacterized protein, transcript variant A (CG8774), mRNA.

Price & Availability↑

CloneID	OFa04787
Clone ID Related Accession (Same CDS sequence)	NM_142017.3
Accession Version	NM_142017.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_142017.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTCCTCA GTGGCTATTG GTTGCAGGTG GTGCTCTCCT GGGCGGTGGT GGCCTTTGCC 
ACGGCCACGG TTGTGGTGGC GGTGCAGAAG GCTCAGCTGG AAAGGGACCT CCGCGATGTG 
CAGGACAAGA TCGATGTGTT CGAGGAGTGG AGCGGAGAGA GCCGCACTCG CATGAAGCGT 
GAAGATACGA CGGATTACCG CCTGCCCACG AATCTGGTGC CCACGCACTA CGAGCTCTAC 
TGGCATCCGG ATCTGGAGAC AGGAAACTTC ACCGGACAAC AGAGGATCAG CATCAAGGTG 
GTGGAGGCCA CTAATCAGAT TATTCTGCAC TCATATCTCC TGGATATCAC AAGTGTCTAT 
GTGCTGAATA GAGAGGTGGA AAAGTTTGAG CTTGAGGAGG AACGTCAGTT CCTGATCATA 
ACTTTGACGG AAGAGCTGGC GGTGGACGCC AGCATTACTC TCGGAATCAT TTTTGGTGGA 
CAGATGAAGG ATAAGCTTGT GGGTCTCTAC AGCAGTACAT ATCTAAACGA GGCAGGAGCT 
ACCAGAACCA TTTCTACGAC GAAATTCGAA CCCACTTACG CGCGTCAGGC CTTCCCATGC 
TTCGATGAAC CAGCCATGAA GGCCACATTC GCCATTACAG TGGTTCATCC ATCGGGATCC 
TACCACGCCG TTTCCAACAT GCAACAAACG GAATCCAATT ATCTGGGTGA CTATACTGAG 
GCCATCTTCG AGACGAGTGT GTCGATGAGC ACCTACCTGG TATGCATCAT TGTGTCCGAC 
TTCGCTTCCC AATCGACCAC GGTGAAGGCC AACGGAATTG GCGAGGACTT CTCCATGCAA 
GCCTATGCCA CCTCGCATCA GATCAACAAG GTCGAGTTTG CTCTGGAGTT CGGACAAGCT 
GTCACGGAGT ACTATATTCA GTACTATAAG GTGCCCTATC CGCTGACCAA ACTGGACATG 
GCCGCCATTC CCGACTTTGC ATCTGGGGCC ATGGAGCACT GGGGATTGGT GACTTACAGA 
GAGACCGCTC TGCTGTACGA TCCCAGTTAC AGTTCCACAG CGAACAAACA ATCGATAGCC 
GGAACACTGG CCCATGAGAT TGCACACCAG TGGTTCGGAA ACCTGGTCAC CATGAAGTGG 
TGGAACGATC TGTGGCTGAA CGAGGGATTC GCCCGCTACA TGCAGTACAA GGGAGTCAAT 
GCTGTGCACC CGGACTGGGG AATGCTGGAG CAATTCCAAA TCGTGGCCCT GCAACCCGTG 
TTGTTATACG ATGCCAAGCT ATCATCCCAT CCGATTGTCC AGAAGGTAGA AAGCCCCGAT 
GAAATTACGG CCATATTCGA CACCATCAGT TACGAAAAGG GAGGATCCGT GATCCGCATG 
CTGGAGACAC TTGTGGGCGC CGAAAAGTTT GAGGAGGCGG TGACCAATTA CCTGGTCAAG 
CATCAGTTCA ATAACACGGT GACCGACGAT TTCCTCACCG AAGTCGAAGC TGTGGTAACT 
GACTTGGATA TCAAGAAACT GATGCTCACC TGGACCGAAC AGATGGGTTA TCCGGTGTTG 
AATGTCTCCA AGGTTGCAGA TGGTAGTTTT AAGGTTACCC AGCAGCGATT CCTCTCCAAT 
CCAGCCAGTT ACGAGGAAGC CCCTTCAGAT AGCGCATACG GATACAAATG GAGTGTTCCG 
ATTACCTGGT TTGCTGATGA TGGTTCAGAG AACAGCTTCA TATACGACTA CGATGTGGAC 
TCTGTGGGTA TTGCCGTGCC CAGCGAGGTG CAGTGGATCA AGTTGAATGT CAACCAAACG 
GGCTACTATC GGGTTAACTA CGAGGAGAGC CTGTGGGCCC TGCTCATCCA GCAGCTAACC 
ACCAGTCCCG CTCGATTCGA AATCGCCGAT CGTGGCCATC TTTTGAACGA TGCCTTCGCC 
CTGGCGGATG CCAACCAACT ATCCTACAAA ATTCCTCTGG ATATGACCGC TTACTTGGCC 
CAGGAGCGCG ACTTCGTGCC CTGGTATGTG GCCTCCAATA AGCTGAGGTC GCTCCATCGC 
AGTCTCATGT TCAGCGAGGG ATACGTGTCG TACCTCACCT ATGCCAGAAG TCTGATCGCT 
GGCGTATACG AGGAGGTGGG TTGGACTGTG GACGCCGACA ATCACTTGAA GAACCGCCTG 
CGTGTTTCCA TTTTGACCGC CGCCTGTGCC CTGGGTGTTC CAGATTGTCT GCAGCAGGCT 
TCCGAGCGCT TCAGCACCTT CCTGGAGAAT CCGACCACTC GACCTTCCCC CGATCTTCGC 
GAGATTGTCT ACTACTACGG CATGCAGCAG TCCACCAGCC AATCGAGCTG GGATCAGTTG 
TTCCAACTCT TTGTGGCCGA AACCGATGCC AGCGAGAAAC TGAAGCTGAT GTACGGATTG 
TCTGGCGTAC GGAACAGCCA GTATCTGTTC GACTTCCTGG CACAGGCCTC CAGTGATGAG 
AGCATCGTTC GCTCCCAGGA CTATTTCACC TGTGTGCAGT ACATAGCCGC CAATCCTGTG 
GGTGAACCGG TGGTCTGGGA ATTCTACCGC GAACAGTGGC CCCAACTCAC CACCCGTTTT 
GGCCTGAACA ACCGCAACTT TGGACGACTT ATCGCCCAGA TTACGGCCAA CTTTGCCAGC 
TCCGTGAAAC TGGAGGAGGT TCAGCACTTC TTCTCCAAGT ACCCCGAATC GGGAGCTGGA 
GCTAACTCCC GGTTGGAGGC CGTGGAAACC ATCAAGTACA ACATCGAATG GCTATCCAGA 
AACGAAGCTG ACATCACCGA CTGGCTAAGT GGAACGGCCT CGCCGCTGAC CAAGAAAAAC 
CAATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650274.2
CDS	35..2863
Translation	MFLSGYWLQVVLSWAVVAFATATVVVAVQKAQLERDLRDVQDKIDVFEEWSGESRTRMKREDTTDYRLPTNLVPTHYELYWHPDLETGNFTGQQRISIKVVEATNQIILHSYLLDITSVYVLNREVEKFELEEERQFLIITLTEELAVDASITLGIIFGGQMKDKLVGLYSSTYLNEAGATRTISTTKFEPTYARQAFPCFDEPAMKATFAITVVHPSGSYHAVSNMQQTESNYLGDYTEAIFETSVSMSTYLVCIIVSDFASQSTTVKANGIGEDFSMQAYATSHQINKVEFALEFGQAVTEYYIQYYKVPYPLTKLDMAAIPDFASGAMEHWGLVTYRETALLYDPSYSSTANKQSIAGTLAHEIAHQWFGNLVTMKWWNDLWLNEGFARYMQYKGVNAVHPDWGMLEQFQIVALQPVLLYDAKLSSHPIVQKVESPDEITAIFDTISYEKGGSVIRMLETLVGAEKFEEAVTNYLVKHQFNNTVTDDFLTEVEAVVTDLDIKKLMLTWTEQMGYPVLNVSKVADGSFKVTQQRFLSNPASYEEAPSDSAYGYKWSVPITWFADDGSENSFIYDYDVDSVGIAVPSEVQWIKLNVNQTGYYRVNYEESLWALLIQQLTTSPARFEIADRGHLLNDAFALADANQLSYKIPLDMTAYLAQERDFVPWYVASNKLRSLHRSLMFSEGYVSYLTYARSLIAGVYEEVGWTVDADNHLKNRLRVSILTAACALGVPDCLQQASERFSTFLENPTTRPSPDLREIVYYYGMQQSTSQSSWDQLFQLFVAETDASEKLKLMYGLSGVRNSQYLFDFLAQASSDESIVRSQDYFTCVQYIAANPVGEPVVWEFYREQWPQLTTRFGLNNRNFGRLIAQITANFASSVKLEEVQHFFSKYPESGAGANSRLEAVETIKYNIEWLSRNEADITDWLSGTASPLTKKNQL

Target ORF information:

RefSeq Version	NM_142017.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG8774), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_142017.3

ORF Insert Sequence:

ATGTTCCTCA GTGGCTATTG GTTGCAGGTG GTGCTCTCCT GGGCGGTGGT GGCCTTTGCC 
ACGGCCACGG TTGTGGTGGC GGTGCAGAAG GCTCAGCTGG AAAGGGACCT CCGCGATGTG 
CAGGACAAGA TCGATGTGTT CGAGGAGTGG AGCGGAGAGA GCCGCACTCG CATGAAGCGT 
GAAGATACGA CGGATTACCG CCTGCCCACG AATCTGGTGC CCACGCACTA CGAGCTCTAC 
TGGCATCCGG ATCTGGAGAC AGGAAACTTC ACCGGACAAC AGAGGATCAG CATCAAGGTG 
GTGGAGGCCA CTAATCAGAT TATTCTGCAC TCATATCTCC TGGATATCAC AAGTGTCTAT 
GTGCTGAATA GAGAGGTGGA AAAGTTTGAG CTTGAGGAGG AACGTCAGTT CCTGATCATA 
ACTTTGACGG AAGAGCTGGC GGTGGACGCC AGCATTACTC TCGGAATCAT TTTTGGTGGA 
CAGATGAAGG ATAAGCTTGT GGGTCTCTAC AGCAGTACAT ATCTAAACGA GGCAGGAGCT 
ACCAGAACCA TTTCTACGAC GAAATTCGAA CCCACTTACG CGCGTCAGGC CTTCCCATGC 
TTCGATGAAC CAGCCATGAA GGCCACATTC GCCATTACAG TGGTTCATCC ATCGGGATCC 
TACCACGCCG TTTCCAACAT GCAACAAACG GAATCCAATT ATCTGGGTGA CTATACTGAG 
GCCATCTTCG AGACGAGTGT GTCGATGAGC ACCTACCTGG TATGCATCAT TGTGTCCGAC 
TTCGCTTCCC AATCGACCAC GGTGAAGGCC AACGGAATTG GCGAGGACTT CTCCATGCAA 
GCCTATGCCA CCTCGCATCA GATCAACAAG GTCGAGTTTG CTCTGGAGTT CGGACAAGCT 
GTCACGGAGT ACTATATTCA GTACTATAAG GTGCCCTATC CGCTGACCAA ACTGGACATG 
GCCGCCATTC CCGACTTTGC ATCTGGGGCC ATGGAGCACT GGGGATTGGT GACTTACAGA 
GAGACCGCTC TGCTGTACGA TCCCAGTTAC AGTTCCACAG CGAACAAACA ATCGATAGCC 
GGAACACTGG CCCATGAGAT TGCACACCAG TGGTTCGGAA ACCTGGTCAC CATGAAGTGG 
TGGAACGATC TGTGGCTGAA CGAGGGATTC GCCCGCTACA TGCAGTACAA GGGAGTCAAT 
GCTGTGCACC CGGACTGGGG AATGCTGGAG CAATTCCAAA TCGTGGCCCT GCAACCCGTG 
TTGTTATACG ATGCCAAGCT ATCATCCCAT CCGATTGTCC AGAAGGTAGA AAGCCCCGAT 
GAAATTACGG CCATATTCGA CACCATCAGT TACGAAAAGG GAGGATCCGT GATCCGCATG 
CTGGAGACAC TTGTGGGCGC CGAAAAGTTT GAGGAGGCGG TGACCAATTA CCTGGTCAAG 
CATCAGTTCA ATAACACGGT GACCGACGAT TTCCTCACCG AAGTCGAAGC TGTGGTAACT 
GACTTGGATA TCAAGAAACT GATGCTCACC TGGACCGAAC AGATGGGTTA TCCGGTGTTG 
AATGTCTCCA AGGTTGCAGA TGGTAGTTTT AAGGTTACCC AGCAGCGATT CCTCTCCAAT 
CCAGCCAGTT ACGAGGAAGC CCCTTCAGAT AGCGCATACG GATACAAATG GAGTGTTCCG 
ATTACCTGGT TTGCTGATGA TGGTTCAGAG AACAGCTTCA TATACGACTA CGATGTGGAC 
TCTGTGGGTA TTGCCGTGCC CAGCGAGGTG CAGTGGATCA AGTTGAATGT CAACCAAACG 
GGCTACTATC GGGTTAACTA CGAGGAGAGC CTGTGGGCCC TGCTCATCCA GCAGCTAACC 
ACCAGTCCCG CTCGATTCGA AATCGCCGAT CGTGGCCATC TTTTGAACGA TGCCTTCGCC 
CTGGCGGATG CCAACCAACT ATCCTACAAA ATTCCTCTGG ATATGACCGC TTACTTGGCC 
CAGGAGCGCG ACTTCGTGCC CTGGTATGTG GCCTCCAATA AGCTGAGGTC GCTCCATCGC 
AGTCTCATGT TCAGCGAGGG ATACGTGTCG TACCTCACCT ATGCCAGAAG TCTGATCGCT 
GGCGTATACG AGGAGGTGGG TTGGACTGTG GACGCCGACA ATCACTTGAA GAACCGCCTG 
CGTGTTTCCA TTTTGACCGC CGCCTGTGCC CTGGGTGTTC CAGATTGTCT GCAGCAGGCT 
TCCGAGCGCT TCAGCACCTT CCTGGAGAAT CCGACCACTC GACCTTCCCC CGATCTTCGC 
GAGATTGTCT ACTACTACGG CATGCAGCAG TCCACCAGCC AATCGAGCTG GGATCAGTTG 
TTCCAACTCT TTGTGGCCGA AACCGATGCC AGCGAGAAAC TGAAGCTGAT GTACGGATTG 
TCTGGCGTAC GGAACAGCCA GTATCTGTTC GACTTCCTGG CACAGGCCTC CAGTGATGAG 
AGCATCGTTC GCTCCCAGGA CTATTTCACC TGTGTGCAGT ACATAGCCGC CAATCCTGTG 
GGTGAACCGG TGGTCTGGGA ATTCTACCGC GAACAGTGGC CCCAACTCAC CACCCGTTTT 
GGCCTGAACA ACCGCAACTT TGGACGACTT ATCGCCCAGA TTACGGCCAA CTTTGCCAGC 
TCCGTGAAAC TGGAGGAGGT TCAGCACTTC TTCTCCAAGT ACCCCGAATC GGGAGCTGGA 
GCTAACTCCC GGTTGGAGGC CGTGGAAACC ATCAAGTACA ACATCGAATG GCTATCCAGA 
AACGAAGCTG ACATCACCGA CTGGCTAAGT GGAACGGCCT CGCCGCTGAC CAAGAAAAAC 
CAATTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG8774 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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