ClC-a cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ClC-a
Entrez Gene ID	41428
Full Name	Chloride channel-a
Synonyms	CG14726,CG31116,CG6942,CLC-2,Clc-a,DmClC-2,DmClC-a,DmClCa,Dmel\CG31116,FBgn0037951
General protein information	Preferred Names Chloride channel-a Names CG31116 gene product from transcript CG31116-RG CG31116-PA CG31116-PC CG31116-PD CG31116-PF CG31116-PG CG31116-PH CG31116-PI ClC-a-PA ClC-a-PC ClC-a-PD ClC-a-PF ClC-a-PG ClC-a-PH ClC-a-PI chloride channel-a
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 86F8-86F8

mRNA and Protein(s)

mRNA	Protein	Name
NM_169432.3	NP_731635.2	chloride channel-a, isoform A
NM_169431.3	NP_731634.2	chloride channel-a, isoform C
NM_176462.2	NP_788639.1	chloride channel-a, isoform D
NM_001104281.2	NP_001097751.2	chloride channel-a, isoform G
NM_001275558.1	NP_001262487.1	chloride channel-a, isoform I
NM_001170121.1	NP_001163592.1	chloride channel-a, isoform H
NM_001104282.3	NP_001097752.1	chloride channel-a, isoform F

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	clh-4	NP_001024885.1
Pan troglodytes (chimpanzee)	CLCN2	XP_003950294.1
Rattus norvegicus (Norway rat)	LOC100912455	XP_003751129.1
Caenorhabditis elegans (roundworm)	clh-3	NP_001022060.1
Canis lupus familiaris (dog)	CLCN2	XP_005639832.1
Bos taurus (cattle)	CLCN2	NP_001095957.1
Rattus norvegicus (Norway rat)	Clcn2	NP_058833.1
Xenopus tropicalis (tropical clawed frog)	clcn2	XP_004914466.1
	CLCN2	XP_002808378.1
Mus musculus (house mouse)	Clcn2	NP_034030.2
Danio rerio (zebrafish)	LOC100333644	XP_002667721.3
Drosophila melanogaster (fruit fly)	ClC-a	NP_001097752.1
Homo sapiens (human)	CLCN2	NP_004357.3
Gallus gallus (chicken)	CLCN2	XP_423073.4
Anopheles gambiae (African malaria mosquito)	GPRMGL4	XP_003436287.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ClC-a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ClC-a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa07781	NM_001275558.1 Latest version!	Drosophila melanogaster chloride channel-a, transcript variant I (ClC-a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa04584	NM_001104281.2 Latest version!	Drosophila melanogaster chloride channel-a, transcript variant G (ClC-a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa04585	NM_001104282.3 Latest version!	Drosophila melanogaster chloride channel-a, transcript variant F (ClC-a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa04586	NM_176462.2 Latest version!	Drosophila melanogaster chloride channel-a, transcript variant D (ClC-a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa04587	NM_169431.3 Latest version!	Drosophila melanogaster chloride channel-a, transcript variant C (ClC-a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa04588	NM_169432.3 Latest version!	Drosophila melanogaster chloride channel-a, transcript variant A (ClC-a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa07780	NM_001170121.1 Latest version!	Drosophila melanogaster chloride channel-a, transcript variant H (ClC-a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ClC-a ( NM_001275558.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262487.1 Drosophila melanogaster chloride channel-a, transcript variant I (ClC-a), mRNA.

Price & Availability↑

CloneID	OFa07781
Clone ID Related Accession (Same CDS sequence)	NM_001275558.1
Accession Version	NM_001275558.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chloride channel-a, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAGCGA CCAGGAACGG CGGCCAGCTG CTCATCGCTC CTTCGCCGCA GACTGCTGCC 
CGTCTGCTGC CCCTGCGCAC CTACTCCAAC GCCTCCTCGC TGGGCCACCA CCATGACAGG 
AGCACCCTGT CCGACGGCGA CGAGGAGGAG GGCGGCCTTG GCTACACCCA CACGCTGATG 
TATGGTCGCT ATACCAAAGA TCTAGGAGAA TTTGCCAAGG ATGAAGCCAG AAAGCTCAAA 
ATACTCGAAA AGCGACGAAA GCAGGAAGAT AAGCAAAGGA ATAAGTGCTG GGAAATATTT 
ACAGGAACTG CTGGGCAAAC ATTCGACCCG GGCGAAGCGG GTATCATCAT GGATCTGGAG 
GCACACGGTG GCCCGACTGG GCGAGGATTG GGTATTCCTG GCCCTACTGG GCATCATTAT 
GGCCCTGCTC TCGTTCATCA TGGACAAGGG CATATCCATA TGTACAAACG CGCGAATTTG 
GCTGTATCGC GATCTGACGT CACAGCCTTT TGTTCAGTAC ATCGCATGGG TCTCACTGCC 
GGTCTGTTTG ATATTGTTCT CAGCCGGCTT TGTCCATCTC ATCGCACCGC AAAGTATAGG 
TTCTGGTATA CCCGAAATGA AGACCATACT GCGCGGCGTT CAATTGAAAG AGTATCTCAC 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262487.1
CDS	809..1474
Translation	MKATRNGGQLLIAPSPQTAARLLPLRTYSNASSLGHHHDRSTLSDGDEEEGGLGYTHTLMYGRYTKDLGEFAKDEARKLKILEKRRKQEDKQRNKCWEIFTGTAGQTFDPGEAGIIMDLEAHGGPTGRGLGIPGPTGHHYGPALVHHGQGHIHMYKRANLAVSRSDVTAFCSVHRMGLTAGLFDIVLSRLCPSHRTAKYRFWYTRNEDHTARRSIERVSHI

Target ORF information:

RefSeq Version	NM_001275558.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chloride channel-a, transcript variant I (ClC-a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275558.1

ORF Insert Sequence:

ATGAAAGCGA CCAGGAACGG CGGCCAGCTG CTCATCGCTC CTTCGCCGCA GACTGCTGCC 
CGTCTGCTGC CCCTGCGCAC CTACTCCAAC GCCTCCTCGC TGGGCCACCA CCATGACAGG 
AGCACCCTGT CCGACGGCGA CGAGGAGGAG GGCGGCCTTG GCTACACCCA CACGCTGATG 
TATGGTCGCT ATACCAAAGA TCTAGGAGAA TTTGCCAAGG ATGAAGCCAG AAAGCTCAAA 
ATACTCGAAA AGCGACGAAA GCAGGAAGAT AAGCAAAGGA ATAAGTGCTG GGAAATATTT 
ACAGGAACTG CTGGGCAAAC ATTCGACCCG GGCGAAGCGG GTATCATCAT GGATCTGGAG 
GCACACGGTG GCCCGACTGG GCGAGGATTG GGTATTCCTG GCCCTACTGG GCATCATTAT 
GGCCCTGCTC TCGTTCATCA TGGACAAGGG CATATCCATA TGTACAAACG CGCGAATTTG 
GCTGTATCGC GATCTGACGT CACAGCCTTT TGTTCAGTAC ATCGCATGGG TCTCACTGCC 
GGTCTGTTTG ATATTGTTCT CAGCCGGCTT TGTCCATCTC ATCGCACCGC AAAGTATAGG 
TTCTGGTATA CCCGAAATGA AGACCATACT GCGCGGCGTT CAATTGAAAG AGTATCTCAC 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ClC-a ( NM_001104281.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097751.2 Drosophila melanogaster chloride channel-a, transcript variant G (ClC-a), mRNA.

Price & Availability↑

CloneID	OFa04584
Clone ID Related Accession (Same CDS sequence)	NM_001104281.2
Accession Version	NM_001104281.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chloride channel-a, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_001104281.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAGCGA CCAGGAACGG CGGCCAGCTG CTCATCGCTC CTTCGCCGCA GACTGCTGCC 
CGTCTGCTGC CCCTGCGCAC CTACTCCAAC GCCTCCTCGC TGGGCCACCA CCATGACAGG 
AGCACCCTGT CCGACGGCGA CGAGGAGGAG GGCGGCCTTG GCTACACCCA CACGCTGATG 
TATGGTCGCT ATACCAAAGA TCTAGGAGAA TTTGCCAAGG ATGAAGCCAG AAAGCTCAAA 
ATACTCGAAA AGCGACGAAA GCAGGAAGAT AAGCAAAGGA ATAAGGAACT GCTGGGCAAA 
CATTCGACCC GGGCGAAGCG GGTATCATCA TGGATCTGGA GGCACACGGT GGCCCGACTG 
GGCGAGGATT GGGTATTCCT GGCCCTACTG GGCATCATTA TGGCCCTGCT CTCGTTCATC 
ATGGACAAGG GCATATCCAT ATGTACAAAC GCGCGAATTT GGCTGTATCG CGATCTGACG 
TCACAGCCTT TTGTTCAGTA CATCGCATGG GTCTCACTGC CGGTCTGTTT GATATTGTTC 
TCAGCCGGCT TTGTCCATCT CATCGCACCG CAAAGTATAG GTTCTGGTAT ACCCGAAATG 
AAGACCATAC TGCGCGGCGT TCAATTGAAA GAGTATCTCA CATTTAAGAC ACTAGTGGCC 
AAGGTAATTG GTTTAACGGC AACTCTGGGC AGCGGTATGC CATTAGGAAA GGAAGGTCCT 
TTCGTACATA TAGCAAGTAT TGTAGCACAA TTATTAAGTA AACTTGTCAC ATCATTCCAA 
GGCATATATG AGAATGAGTC GCGAAACTCT GAAATGTTGG CGGCAGCCTG TGCTGTCGGT 
GTGGGCGCCT GTTTTGCCGC TCCAGTCGGT GGTGTGCTCT TCAGCATAGA GGTCACCACC 
ACGTACTTCG CTGTGCGTAA CTACTGGCGC GGATTCTTTG CGGCTGTGTG CGGCGCCACT 
GTCTTTCGGC TCCTGGCCGT TTGGTTCCAA AACGCGGACA CCGTCCGCGC CCTGTTCCTC 
ACGAACTTCA CCACCGAGTT TCCCTTCGAT CCTCAGGAGC TGTTCGTCTT TGCTTTGATT 
GGACTTGTGT GCGGCTTGGG TGGCGCATCA TATGTGTGGG TCCATCGGCG ATATGTCCTC 
TTCATGAGAT CCAACAAGCG GATGAACAAG TTCCTGCAGA AAAATCGCTT TTTGTATCCG 
GGATTCCTGG CTCTGCTGGT GTCCAGCATC TCGTTTCCAC TGGGCACGGG TCAGTTCCTG 
GCCGGCGAAC TAAGTACCCA CGAGCAGGTG ACACAGCTTT TTAGCAATTT CACGTGGTCA 
CGAGATGATC TTACTGTGGA GCAGGCGGCT GTAGTGACCC ACTGGATGAC CAGTTACACC 
AGCGTCTTTG GCAACCTTGT CATCTACACC CTCTTTACGT TTGTGGTCTC CATTATCGCA 
TCCACGATAC CAGTTCCGTC GGGCATGTTC ATTCCGGTTT TCAAGATCGG TGCTGGCTTT 
GGTCGTCTGG TGGGCGAGTT CATGGCGGTG ACATTTCCCC ACGGCGTCCG GTATGGCGGT 
CGTCTCTCGC CCATCATGCC CGGAGGCTAT GCCGTCGTCG GAGCGGCCGC CTTTTCTGGC 
TCCGTGACCC ACACGGTGTC TGTGGCGGTG ATCATTTTCG AGATGACTGG TCAGATTACG 
CACGTGGTAC CCGTCATGAT TGCTGTGCTG GTGGCCAATG CCGTGGCGGC GCTGCTCCAA 
CCGTCGATAT ACGACAGTAT TATATTGATT AAGAAGCTGC CCTACCTGCC GGATCTGCTG 
CCCTCCAGTT CCGGGATGTA CAGCATATTC GTCGAGGACT TTATGGTGCG GGATGTAAAG 
TACATATGGC ACGGCATCTC GTATCAGAAG CTCAAAGAAG TCCTCAAGCT GAACAAGACG 
CTGAGATCCT TGCCACTGGT GGACAGCCCG GACAACATGA TCCTGCTGGG TTCCGTGCAG 
CGGTACGAGC TGATCAAAAT GATCGAGAAG CACATTGGAC GCGAGAAGCG AATGGAGGTG 
GCCCAGAAGT GGCAGAAGGA GGCTCAGGAA AGAGCCCTCG AGGAGGAGAA GAAGAAACAG 
GAAGTGGAGC TCAAGATGCG GCGACCATCT CGATTCGAGG TCCTTCCAGC TCCGGATATT 
CTCAGTTTAC GCCAGATTGC CAACGACGAA ATGCTGCCGC CCAAGAAAAG GGCGGAGACA 
ATGCACGGTT CGCTGGCGCC CAGGAAGTCC ATTTTGAAGA AGACCAACTC CTTCAACCTG 
AAGACCTACG CGCAGCCCAT GGGGCACAGT CCCAGCATCA CGCCCTACAC CACGATCACC 
GGCAATTCCG AGTTCCGCAT TCGCTCAGCC TTCGAGGCCA TATTTAAGAA GTCCACCACG 
CTCCAGGACG TACAGCCGGA TCCGGAAACG GGTTCCCTCT CCCCGGCGGC AAGCAACCAC 
GAGGTGGAGG TGCCGCGAAC TCCTAGCACT CCCGGTGTTT CCAAAAAGGT TCAGCTGCCC 
AGGGAACGTG TTATTGACAT GTCACCGGAG GATCAGAAGC AATGGGAGCT CGAGGAAATG 
TTGAAGCCCA TCGATTTGCA GAAGGCCAAT GTCCACATCG ATCCCTCGCC ATTTCAGCTG 
GTGGAACGCA CCTCCATACT CAAGGTTCAC TCGTTGTTTT CCATGGTGGG TATTAACCAC 
GCCTATGTCA CCAAAATCGG AAGACTTGTT GGTGTCGTGG GACTCAAAGA ATTGCGAAAA 
GCCATCGAGG ACATTAATAG CAATAGTTTC GTGCCCCCCA CACGGGATGA GGATGCGGAT 
GAGAAGCCGG CGGTGGAGAA ACCTCTGCTG TCGACGAACT CTAGTGATAA GGCCGTGGAC 
ATGACCGTCA CCTCCATGGA CTCGGCACTA TCCAATTCCG AGAACTGTTC GGACATTGAA 
ATGGAGCACA TAAAGCACAC GGATAAGGGC ACTGTATCGC TCACCATGCC GCCACAGGAA 
TCTAAGCAAA GTCCATCGGC GGACAAAAGT AACACAGAAA ACGGAAATCA TGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097751.2
CDS	777..3833
Translation	MKATRNGGQLLIAPSPQTAARLLPLRTYSNASSLGHHHDRSTLSDGDEEEGGLGYTHTLMYGRYTKDLGEFAKDEARKLKILEKRRKQEDKQRNKELLGKHSTRAKRVSSWIWRHTVARLGEDWVFLALLGIIMALLSFIMDKGISICTNARIWLYRDLTSQPFVQYIAWVSLPVCLILFSAGFVHLIAPQSIGSGIPEMKTILRGVQLKEYLTFKTLVAKVIGLTATLGSGMPLGKEGPFVHIASIVAQLLSKLVTSFQGIYENESRNSEMLAAACAVGVGACFAAPVGGVLFSIEVTTTYFAVRNYWRGFFAAVCGATVFRLLAVWFQNADTVRALFLTNFTTEFPFDPQELFVFALIGLVCGLGGASYVWVHRRYVLFMRSNKRMNKFLQKNRFLYPGFLALLVSSISFPLGTGQFLAGELSTHEQVTQLFSNFTWSRDDLTVEQAAVVTHWMTSYTSVFGNLVIYTLFTFVVSIIASTIPVPSGMFIPVFKIGAGFGRLVGEFMAVTFPHGVRYGGRLSPIMPGGYAVVGAAAFSGSVTHTVSVAVIIFEMTGQITHVVPVMIAVLVANAVAALLQPSIYDSIILIKKLPYLPDLLPSSSGMYSIFVEDFMVRDVKYIWHGISYQKLKEVLKLNKTLRSLPLVDSPDNMILLGSVQRYELIKMIEKHIGREKRMEVAQKWQKEAQERALEEEKKKQEVELKMRRPSRFEVLPAPDILSLRQIANDEMLPPKKRAETMHGSLAPRKSILKKTNSFNLKTYAQPMGHSPSITPYTTITGNSEFRIRSAFEAIFKKSTTLQDVQPDPETGSLSPAASNHEVEVPRTPSTPGVSKKVQLPRERVIDMSPEDQKQWELEEMLKPIDLQKANVHIDPSPFQLVERTSILKVHSLFSMVGINHAYVTKIGRLVGVVGLKELRKAIEDINSNSFVPPTRDEDADEKPAVEKPLLSTNSSDKAVDMTVTSMDSALSNSENCSDIEMEHIKHTDKGTVSLTMPPQESKQSPSADKSNTENGNHA

Target ORF information:

RefSeq Version	NM_001104281.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chloride channel-a, transcript variant G (ClC-a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104281.2

ORF Insert Sequence:

ATGAAAGCGA CCAGGAACGG CGGCCAGCTG CTCATCGCTC CTTCGCCGCA GACTGCTGCC 
CGTCTGCTGC CCCTGCGCAC CTACTCCAAC GCCTCCTCGC TGGGCCACCA CCATGACAGG 
AGCACCCTGT CCGACGGCGA CGAGGAGGAG GGCGGCCTTG GCTACACCCA CACGCTGATG 
TATGGTCGCT ATACCAAAGA TCTAGGAGAA TTTGCCAAGG ATGAAGCCAG AAAGCTCAAA 
ATACTCGAAA AGCGACGAAA GCAGGAAGAT AAGCAAAGGA ATAAGGAACT GCTGGGCAAA 
CATTCGACCC GGGCGAAGCG GGTATCATCA TGGATCTGGA GGCACACGGT GGCCCGACTG 
GGCGAGGATT GGGTATTCCT GGCCCTACTG GGCATCATTA TGGCCCTGCT CTCGTTCATC 
ATGGACAAGG GCATATCCAT ATGTACAAAC GCGCGAATTT GGCTGTATCG CGATCTGACG 
TCACAGCCTT TTGTTCAGTA CATCGCATGG GTCTCACTGC CGGTCTGTTT GATATTGTTC 
TCAGCCGGCT TTGTCCATCT CATCGCACCG CAAAGTATAG GTTCTGGTAT ACCCGAAATG 
AAGACCATAC TGCGCGGCGT TCAATTGAAA GAGTATCTCA CATTTAAGAC ACTAGTGGCC 
AAGGTAATTG GTTTAACGGC AACTCTGGGC AGCGGTATGC CATTAGGAAA GGAAGGTCCT 
TTCGTACATA TAGCAAGTAT TGTAGCACAA TTATTAAGTA AACTTGTCAC ATCATTCCAA 
GGCATATATG AGAATGAGTC GCGAAACTCT GAAATGTTGG CGGCAGCCTG TGCTGTCGGT 
GTGGGCGCCT GTTTTGCCGC TCCAGTCGGT GGTGTGCTCT TCAGCATAGA GGTCACCACC 
ACGTACTTCG CTGTGCGTAA CTACTGGCGC GGATTCTTTG CGGCTGTGTG CGGCGCCACT 
GTCTTTCGGC TCCTGGCCGT TTGGTTCCAA AACGCGGACA CCGTCCGCGC CCTGTTCCTC 
ACGAACTTCA CCACCGAGTT TCCCTTCGAT CCTCAGGAGC TGTTCGTCTT TGCTTTGATT 
GGACTTGTGT GCGGCTTGGG TGGCGCATCA TATGTGTGGG TCCATCGGCG ATATGTCCTC 
TTCATGAGAT CCAACAAGCG GATGAACAAG TTCCTGCAGA AAAATCGCTT TTTGTATCCG 
GGATTCCTGG CTCTGCTGGT GTCCAGCATC TCGTTTCCAC TGGGCACGGG TCAGTTCCTG 
GCCGGCGAAC TAAGTACCCA CGAGCAGGTG ACACAGCTTT TTAGCAATTT CACGTGGTCA 
CGAGATGATC TTACTGTGGA GCAGGCGGCT GTAGTGACCC ACTGGATGAC CAGTTACACC 
AGCGTCTTTG GCAACCTTGT CATCTACACC CTCTTTACGT TTGTGGTCTC CATTATCGCA 
TCCACGATAC CAGTTCCGTC GGGCATGTTC ATTCCGGTTT TCAAGATCGG TGCTGGCTTT 
GGTCGTCTGG TGGGCGAGTT CATGGCGGTG ACATTTCCCC ACGGCGTCCG GTATGGCGGT 
CGTCTCTCGC CCATCATGCC CGGAGGCTAT GCCGTCGTCG GAGCGGCCGC CTTTTCTGGC 
TCCGTGACCC ACACGGTGTC TGTGGCGGTG ATCATTTTCG AGATGACTGG TCAGATTACG 
CACGTGGTAC CCGTCATGAT TGCTGTGCTG GTGGCCAATG CCGTGGCGGC GCTGCTCCAA 
CCGTCGATAT ACGACAGTAT TATATTGATT AAGAAGCTGC CCTACCTGCC GGATCTGCTG 
CCCTCCAGTT CCGGGATGTA CAGCATATTC GTCGAGGACT TTATGGTGCG GGATGTAAAG 
TACATATGGC ACGGCATCTC GTATCAGAAG CTCAAAGAAG TCCTCAAGCT GAACAAGACG 
CTGAGATCCT TGCCACTGGT GGACAGCCCG GACAACATGA TCCTGCTGGG TTCCGTGCAG 
CGGTACGAGC TGATCAAAAT GATCGAGAAG CACATTGGAC GCGAGAAGCG AATGGAGGTG 
GCCCAGAAGT GGCAGAAGGA GGCTCAGGAA AGAGCCCTCG AGGAGGAGAA GAAGAAACAG 
GAAGTGGAGC TCAAGATGCG GCGACCATCT CGATTCGAGG TCCTTCCAGC TCCGGATATT 
CTCAGTTTAC GCCAGATTGC CAACGACGAA ATGCTGCCGC CCAAGAAAAG GGCGGAGACA 
ATGCACGGTT CGCTGGCGCC CAGGAAGTCC ATTTTGAAGA AGACCAACTC CTTCAACCTG 
AAGACCTACG CGCAGCCCAT GGGGCACAGT CCCAGCATCA CGCCCTACAC CACGATCACC 
GGCAATTCCG AGTTCCGCAT TCGCTCAGCC TTCGAGGCCA TATTTAAGAA GTCCACCACG 
CTCCAGGACG TACAGCCGGA TCCGGAAACG GGTTCCCTCT CCCCGGCGGC AAGCAACCAC 
GAGGTGGAGG TGCCGCGAAC TCCTAGCACT CCCGGTGTTT CCAAAAAGGT TCAGCTGCCC 
AGGGAACGTG TTATTGACAT GTCACCGGAG GATCAGAAGC AATGGGAGCT CGAGGAAATG 
TTGAAGCCCA TCGATTTGCA GAAGGCCAAT GTCCACATCG ATCCCTCGCC ATTTCAGCTG 
GTGGAACGCA CCTCCATACT CAAGGTTCAC TCGTTGTTTT CCATGGTGGG TATTAACCAC 
GCCTATGTCA CCAAAATCGG AAGACTTGTT GGTGTCGTGG GACTCAAAGA ATTGCGAAAA 
GCCATCGAGG ACATTAATAG CAATAGTTTC GTGCCCCCCA CACGGGATGA GGATGCGGAT 
GAGAAGCCGG CGGTGGAGAA ACCTCTGCTG TCGACGAACT CTAGTGATAA GGCCGTGGAC 
ATGACCGTCA CCTCCATGGA CTCGGCACTA TCCAATTCCG AGAACTGTTC GGACATTGAA 
ATGGAGCACA TAAAGCACAC GGATAAGGGC ACTGTATCGC TCACCATGCC GCCACAGGAA 
TCTAAGCAAA GTCCATCGGC GGACAAAAGT AACACAGAAA ACGGAAATCA TGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ClC-a ( NM_001104282.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097752.1 Drosophila melanogaster chloride channel-a, transcript variant F (ClC-a), mRNA.

Price & Availability↑

CloneID	OFa04585
Clone ID Related Accession (Same CDS sequence)	NM_001104282.3
Accession Version	NM_001104282.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chloride channel-a, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001104282.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTTATT TCGGCGATCG GCAACGCGAT CGCAATCGCG ATCGGAGTAA TCAGAAAGTC 
GAGCGAATCA TTCACGATGA AGAGTTTGGC GAGGAGAACG TCGAGTTGGT GGACTCGGAG 
TGGGCGGACT TCGAGAAGTT CATTTGCCAG TTGCGCAAGC GGCGGAACAG TGCTATGTCC 
ATGGAGGAGG AACTGCGTCA CGTGCAGCGG CATCCGAAGA TCAAGTCGCA CGCCTTCTAT 
CCGTGCCCGC CGCCAGCTGA AAATGCTCGG GATTCGGACT CCTCGGACGA TGATGATCCC 
ATCGGATACA TCGATACTCT TATGTATGGT CGCTATACCA AAGATCTAGG AGAATTTGCC 
AAGGATGAAG CCAGAAAGCT CAAAATACTC GAAAAGCGAC GAAAGCAGGA AGATAAGCAA 
AGGAATAAGG AACTGCTGGG CAAACATTCG ACCCGGGCGA AGCGGGTATC ATCATGGATC 
TGGAGGCACA CGGTGGCCCG ACTGGGCGAG GATTGGGTAT TCCTGGCCCT ACTGGGCATC 
ATTATGGCCC TGCTCTCGTT CATCATGGAC AAGGGCATAT CCATATGTAC AAACGCGCGA 
ATTTGGCTGT ATCGCGATCT GACGTCACAG CCTTTTGTTC AGTACATCGC ATGGGTCTCA 
CTGCCGGTCT GTTTGATATT GTTCTCAGCC GGCTTTGTCC ATCTCATCGC ACCGCAAAGT 
ATAGGTTCTG GTATACCCGA AATGAAGACC ATACTGCGCG GCGTTCAATT GAAAGAGTAT 
CTCACATTTA AGACACTAGT GGCCAAGGTA ATTGGTTTAA CGGCAACTCT GGGCAGCGGT 
ATGCCATTAG GAAAGGAAGG TCCTTTCGTA CATATAGCAA GTATTGTAGC ACAATTATTA 
AGTAAACTTG TCACATCATT CCAAGGCATA TATGAGAATG AGTCGCGAAA CTCTGAAATG 
TTGGCGGCAG CCTGTGCTGT CGGTGTGGGC GCCTGTTTTG CCGCTCCAGT CGGTGGTGTG 
CTCTTCAGCA TAGAGGTCAC CACCACGTAC TTCGCTGTGC GTAACTACTG GCGCGGATTC 
TTTGCGGCTG TGTGCGGCGC CACTGTCTTT CGGCTCCTGG CCGTTTGGTT CCAAAACGCG 
GACACCGTCC GCGCCCTGTT CCTCACGAAC TTCACCACCG AGTTTCCCTT CGATCCTCAG 
GAGCTGTTCG TCTTTGCTTT GATTGGACTT GTGTGCGGCT TGGGTGGCGC ATCATATGTG 
TGGGTCCATC GGCGATATGT CCTCTTCATG AGATCCAACA AGCGGATGAA CAAGTTCCTG 
CAGAAAAATC GCTTTTTGTA TCCGGGATTC CTGGCTCTGC TGGTGTCCAG CATCTCGTTT 
CCACTGGGCA CGGGTCAGTT CCTGGCCGGC GAACTAAGTA CCCACGAGCA GGTGACACAG 
CTTTTTAGCA ATTTCACGTG GTCACGAGAT GATCTTACTG TGGAGCAGGC GGCTGTAGTG 
ACCCACTGGA TGACCAGTTA CACCAGCGTC TTTGGCAACC TTGTCATCTA CACCCTCTTT 
ACGTTTGTGG TCTCCATTAT CGCATCCACG ATACCAGTTC CGTCGGGCAT GTTCATTCCG 
GTTTTCAAGA TCGGTGCTGG CTTTGGTCGT CTGGTGGGCG AGTTCATGGC GGTGACATTT 
CCCCACGGCG TCCGGTATGG CGGTCGTCTC TCGCCCATCA TGCCCGGAGG CTATGCCGTC 
GTCGGAGCGG CCGCCTTTTC TGGCTCCGTG ACCCACACGG TGTCTGTGGC GGTGATCATT 
TTCGAGATGA CTGGTCAGAT TACGCACGTG GTACCCGTCA TGATTGCTGT GCTGGTGGCC 
AATGCCGTGG CGGCGCTGCT CCAACCGTCG ATATACGACA GTATTATATT GATTAAGAAG 
CTGCCCTACC TGCCGGATCT GCTGCCCTCC AGTTCCGGGA TGTACAGCAT ATTCGTCGAG 
GACTTTATGG TGCGGGATGT AAAGTACATA TGGCACGGCA TCTCGTATCA GAAGCTCAAA 
GAAGTCCTCA AGCTGAACAA GACGCTGAGA TCCTTGCCAC TGGTGGACAG CCCGGACAAC 
ATGATCCTGC TGGGTTCCGT GCAGCGGTAC GAGCTGATCA AAATGATCGA GAAGCACATT 
GGACGCGAGA AGCGAATGGA GGTGGCCCAG AAGTGGCAGA AGGAGGCTCA GGAAAGAGCC 
CTCGAGGAGG AGAAGAAGAA ACAGGAAGTG GAGCTCAAGA TGCGGCGACC ATCTCGATTC 
GAGGTCCTTC CAGCTCCGGA TATTCTCAGT TTACGCCAGA TTGCCAACGA CGAAATGCTG 
CCGCCCAAGA AAAGGGCGGA GACAATGCAC GGTTCGCTGG CGCCCAGGAA GTCCATTTTG 
AAGAAGACCA ACTCCTTCAA CCTGAAGACC TACGCGCAGC CCATGGGGCA CAGTCCCAGC 
ATCACGCCCT ACACCACGAT CACCGGCAAT TCCGAGTTCC GCATTCGCTC AGCCTTCGAG 
GCCATATTTA AGAAGTCCAC CACGCTCCAG GACGTACAGC CGGATCCGGA AACGGGTTCC 
CTCTCCCCGG CGGCAAGCAA CCACGAGGTG GAGGTGCCGC GAACTCCTAG CACTCCCGGT 
GTTTCCAAAA AGGTTCAGCT GCCCAGGGAA CGTGTTATTG ACATGTCACC GGAGGATCAG 
AAGCAATGGG AGCTCGAGGA AATGTTGAAG CCCATCGATT TGCAGAAGGC CAATGTCCAC 
ATCGATCCCT CGCCATTTCA GCTGGTGGAA CGCACCTCCA TACTCAAGGT TCACTCGTTG 
TTTTCCATGG TGGGTATTAA CCACGCCTAT GTCACCAAAA TCGGAAGACT TGTTGGTGTC 
GTGGGACTCA AAGAATTGCG AAAAGCCATC GAGGACATTA ATAGCAATAG TTTCGTGCCC 
CCCACACGGG ATGAGGATGC GGATGAGAAG CCGGCGGTGG AGAAACCTCT GCTGTCGACG 
AACTCTAGTG ATAAGGCCGT GGACATGACC GTCACCTCCA TGGACTCGGC ACTATCCAAT 
TCCGAGAACT GTTCGGACAT TGAAATGGAG CACATAAAGC ACACGGATAA GGGCACTGTA 
TCGCTCACCA TGCCGCCACA GGAATCTAAG CAAAGTCCAT CGGCGGACAA AAGTAACACA 
GAAAACGGAA ATCATGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097752.1
CDS	215..3415
Translation	MVYFGDRQRDRNRDRSNQKVERIIHDEEFGEENVELVDSEWADFEKFICQLRKRRNSAMSMEEELRHVQRHPKIKSHAFYPCPPPAENARDSDSSDDDDPIGYIDTLMYGRYTKDLGEFAKDEARKLKILEKRRKQEDKQRNKELLGKHSTRAKRVSSWIWRHTVARLGEDWVFLALLGIIMALLSFIMDKGISICTNARIWLYRDLTSQPFVQYIAWVSLPVCLILFSAGFVHLIAPQSIGSGIPEMKTILRGVQLKEYLTFKTLVAKVIGLTATLGSGMPLGKEGPFVHIASIVAQLLSKLVTSFQGIYENESRNSEMLAAACAVGVGACFAAPVGGVLFSIEVTTTYFAVRNYWRGFFAAVCGATVFRLLAVWFQNADTVRALFLTNFTTEFPFDPQELFVFALIGLVCGLGGASYVWVHRRYVLFMRSNKRMNKFLQKNRFLYPGFLALLVSSISFPLGTGQFLAGELSTHEQVTQLFSNFTWSRDDLTVEQAAVVTHWMTSYTSVFGNLVIYTLFTFVVSIIASTIPVPSGMFIPVFKIGAGFGRLVGEFMAVTFPHGVRYGGRLSPIMPGGYAVVGAAAFSGSVTHTVSVAVIIFEMTGQITHVVPVMIAVLVANAVAALLQPSIYDSIILIKKLPYLPDLLPSSSGMYSIFVEDFMVRDVKYIWHGISYQKLKEVLKLNKTLRSLPLVDSPDNMILLGSVQRYELIKMIEKHIGREKRMEVAQKWQKEAQERALEEEKKKQEVELKMRRPSRFEVLPAPDILSLRQIANDEMLPPKKRAETMHGSLAPRKSILKKTNSFNLKTYAQPMGHSPSITPYTTITGNSEFRIRSAFEAIFKKSTTLQDVQPDPETGSLSPAASNHEVEVPRTPSTPGVSKKVQLPRERVIDMSPEDQKQWELEEMLKPIDLQKANVHIDPSPFQLVERTSILKVHSLFSMVGINHAYVTKIGRLVGVVGLKELRKAIEDINSNSFVPPTRDEDADEKPAVEKPLLSTNSSDKAVDMTVTSMDSALSNSENCSDIEMEHIKHTDKGTVSLTMPPQESKQSPSADKSNTENGNHA

Target ORF information:

RefSeq Version	NM_001104282.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chloride channel-a, transcript variant F (ClC-a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104282.3

ORF Insert Sequence:

ATGGTTTATT TCGGCGATCG GCAACGCGAT CGCAATCGCG ATCGGAGTAA TCAGAAAGTC 
GAGCGAATCA TTCACGATGA AGAGTTTGGC GAGGAGAACG TCGAGTTGGT GGACTCGGAG 
TGGGCGGACT TCGAGAAGTT CATTTGCCAG TTGCGCAAGC GGCGGAACAG TGCTATGTCC 
ATGGAGGAGG AACTGCGTCA CGTGCAGCGG CATCCGAAGA TCAAGTCGCA CGCCTTCTAT 
CCGTGCCCGC CGCCAGCTGA AAATGCTCGG GATTCGGACT CCTCGGACGA TGATGATCCC 
ATCGGATACA TCGATACTCT TATGTATGGT CGCTATACCA AAGATCTAGG AGAATTTGCC 
AAGGATGAAG CCAGAAAGCT CAAAATACTC GAAAAGCGAC GAAAGCAGGA AGATAAGCAA 
AGGAATAAGG AACTGCTGGG CAAACATTCG ACCCGGGCGA AGCGGGTATC ATCATGGATC 
TGGAGGCACA CGGTGGCCCG ACTGGGCGAG GATTGGGTAT TCCTGGCCCT ACTGGGCATC 
ATTATGGCCC TGCTCTCGTT CATCATGGAC AAGGGCATAT CCATATGTAC AAACGCGCGA 
ATTTGGCTGT ATCGCGATCT GACGTCACAG CCTTTTGTTC AGTACATCGC ATGGGTCTCA 
CTGCCGGTCT GTTTGATATT GTTCTCAGCC GGCTTTGTCC ATCTCATCGC ACCGCAAAGT 
ATAGGTTCTG GTATACCCGA AATGAAGACC ATACTGCGCG GCGTTCAATT GAAAGAGTAT 
CTCACATTTA AGACACTAGT GGCCAAGGTA ATTGGTTTAA CGGCAACTCT GGGCAGCGGT 
ATGCCATTAG GAAAGGAAGG TCCTTTCGTA CATATAGCAA GTATTGTAGC ACAATTATTA 
AGTAAACTTG TCACATCATT CCAAGGCATA TATGAGAATG AGTCGCGAAA CTCTGAAATG 
TTGGCGGCAG CCTGTGCTGT CGGTGTGGGC GCCTGTTTTG CCGCTCCAGT CGGTGGTGTG 
CTCTTCAGCA TAGAGGTCAC CACCACGTAC TTCGCTGTGC GTAACTACTG GCGCGGATTC 
TTTGCGGCTG TGTGCGGCGC CACTGTCTTT CGGCTCCTGG CCGTTTGGTT CCAAAACGCG 
GACACCGTCC GCGCCCTGTT CCTCACGAAC TTCACCACCG AGTTTCCCTT CGATCCTCAG 
GAGCTGTTCG TCTTTGCTTT GATTGGACTT GTGTGCGGCT TGGGTGGCGC ATCATATGTG 
TGGGTCCATC GGCGATATGT CCTCTTCATG AGATCCAACA AGCGGATGAA CAAGTTCCTG 
CAGAAAAATC GCTTTTTGTA TCCGGGATTC CTGGCTCTGC TGGTGTCCAG CATCTCGTTT 
CCACTGGGCA CGGGTCAGTT CCTGGCCGGC GAACTAAGTA CCCACGAGCA GGTGACACAG 
CTTTTTAGCA ATTTCACGTG GTCACGAGAT GATCTTACTG TGGAGCAGGC GGCTGTAGTG 
ACCCACTGGA TGACCAGTTA CACCAGCGTC TTTGGCAACC TTGTCATCTA CACCCTCTTT 
ACGTTTGTGG TCTCCATTAT CGCATCCACG ATACCAGTTC CGTCGGGCAT GTTCATTCCG 
GTTTTCAAGA TCGGTGCTGG CTTTGGTCGT CTGGTGGGCG AGTTCATGGC GGTGACATTT 
CCCCACGGCG TCCGGTATGG CGGTCGTCTC TCGCCCATCA TGCCCGGAGG CTATGCCGTC 
GTCGGAGCGG CCGCCTTTTC TGGCTCCGTG ACCCACACGG TGTCTGTGGC GGTGATCATT 
TTCGAGATGA CTGGTCAGAT TACGCACGTG GTACCCGTCA TGATTGCTGT GCTGGTGGCC 
AATGCCGTGG CGGCGCTGCT CCAACCGTCG ATATACGACA GTATTATATT GATTAAGAAG 
CTGCCCTACC TGCCGGATCT GCTGCCCTCC AGTTCCGGGA TGTACAGCAT ATTCGTCGAG 
GACTTTATGG TGCGGGATGT AAAGTACATA TGGCACGGCA TCTCGTATCA GAAGCTCAAA 
GAAGTCCTCA AGCTGAACAA GACGCTGAGA TCCTTGCCAC TGGTGGACAG CCCGGACAAC 
ATGATCCTGC TGGGTTCCGT GCAGCGGTAC GAGCTGATCA AAATGATCGA GAAGCACATT 
GGACGCGAGA AGCGAATGGA GGTGGCCCAG AAGTGGCAGA AGGAGGCTCA GGAAAGAGCC 
CTCGAGGAGG AGAAGAAGAA ACAGGAAGTG GAGCTCAAGA TGCGGCGACC ATCTCGATTC 
GAGGTCCTTC CAGCTCCGGA TATTCTCAGT TTACGCCAGA TTGCCAACGA CGAAATGCTG 
CCGCCCAAGA AAAGGGCGGA GACAATGCAC GGTTCGCTGG CGCCCAGGAA GTCCATTTTG 
AAGAAGACCA ACTCCTTCAA CCTGAAGACC TACGCGCAGC CCATGGGGCA CAGTCCCAGC 
ATCACGCCCT ACACCACGAT CACCGGCAAT TCCGAGTTCC GCATTCGCTC AGCCTTCGAG 
GCCATATTTA AGAAGTCCAC CACGCTCCAG GACGTACAGC CGGATCCGGA AACGGGTTCC 
CTCTCCCCGG CGGCAAGCAA CCACGAGGTG GAGGTGCCGC GAACTCCTAG CACTCCCGGT 
GTTTCCAAAA AGGTTCAGCT GCCCAGGGAA CGTGTTATTG ACATGTCACC GGAGGATCAG 
AAGCAATGGG AGCTCGAGGA AATGTTGAAG CCCATCGATT TGCAGAAGGC CAATGTCCAC 
ATCGATCCCT CGCCATTTCA GCTGGTGGAA CGCACCTCCA TACTCAAGGT TCACTCGTTG 
TTTTCCATGG TGGGTATTAA CCACGCCTAT GTCACCAAAA TCGGAAGACT TGTTGGTGTC 
GTGGGACTCA AAGAATTGCG AAAAGCCATC GAGGACATTA ATAGCAATAG TTTCGTGCCC 
CCCACACGGG ATGAGGATGC GGATGAGAAG CCGGCGGTGG AGAAACCTCT GCTGTCGACG 
AACTCTAGTG ATAAGGCCGT GGACATGACC GTCACCTCCA TGGACTCGGC ACTATCCAAT 
TCCGAGAACT GTTCGGACAT TGAAATGGAG CACATAAAGC ACACGGATAA GGGCACTGTA 
TCGCTCACCA TGCCGCCACA GGAATCTAAG CAAAGTCCAT CGGCGGACAA AAGTAACACA 
GAAAACGGAA ATCATGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ClC-a ( NM_176462.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_788639.1 Drosophila melanogaster chloride channel-a, transcript variant D (ClC-a), mRNA.

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CloneID	OFa04586
Clone ID Related Accession (Same CDS sequence)	NM_176462.2
Accession Version	NM_176462.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chloride channel-a, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_176462.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTTATT TCGGCGATCG GCAACGCGAT CGCAATCGCG ATCGGAGTAA TCAGAAAGTC 
GAGCGAATCA TTCACGATGA AGAGTTTGGC GAGGAGAACG TCGAGTTGGT GGACTCGGAG 
TGGGCGGACT TCGAGAAGTT CATTTGCCAG TTGCGCAAGC GGCGGAACAG TGCTATGTCC 
ATGGAGGAGG AACTGCGTCA CGTGCAGCGG CATCCGAAGA TCAAGTCGCA CGCCTTCTAT 
CCGTGCCCGC CGCCAGCTGA AAATGCTCGG GATTCGGACT CCTCGGACGA TGATGATCCC 
ATCGGATACA TCGATACTCT TATGTATGGT CGCTATACCA AAGATCTAGG AGAATTTGCC 
AAGGATGAAG CCAGAAAGCT CAAAATACTC GAAAAGCGAC GAAAGCAGGA AGATAAGCAA 
AGGAATAAGG AACTGCTGGG CAAACATTCG ACCCGGGCGA AGCGGGTATC ATCATGGATC 
TGGAGGCACA CGGTGGCCCG ACTGGGCGAG GATTGGGTAT TCCTGGCCCT ACTGGGCATC 
ATTATGGCCC TGCTCTCGTT CATCATGGAC AAGGGCATAT CCATATGTAC AAACGCGCGA 
ATTTGGCTGT ATCGCGATCT GACGTCACAG CCTTTTGTTC AGTACATCGC ATGGGTCTCA 
CTGCCGGTCT GTTTGATATT GTTCTCAGCC GGCTTTGTCC ATCTCATCGC ACCGCAAAGT 
ATAGGTTCTG GTATACCCGA AATGAAGACC ATACTGCGCG GCGTTCAATT GAAAGAGTAT 
CTCACATTTA AGACACTAGT GGCCAAGGTA ATTGGTTTAA CGGCAACTCT GGGCAGCGGT 
ATGCCATTAG GAAAGGAAGG TCCTTTCGTA CATATAGCAA GTATTGTAGC ACAATTATTA 
AGTAAACTTG TCACATCATT CCAAGGCATA TATGAGAATG AGTCGCGAAA CTCTGAAATG 
TTGGCGGCAG CCTGTGCTGT CGGTGTGGGC GCCTGTTTTG CCGCTCCAGT CGGTGGTGTG 
CTCTTCAGCA TAGAGGTCAC CACCACGTAC TTCGCTGTGC GTAACTACTG GCGCGGATTC 
TTTGCGGCTG TGTGCGGCGC CACTGTCTTT CGGCTCCTGG CCGTTTGGTT CCAAAACGCG 
GACACCGTCC GCGCCCTGTT CCTCACGAAC TTCACCACCG AGTTTCCCTT CGATCCTCAG 
GAGCTGTTCG TCTTTGCTTT GATTGGACTT GTGTGCGGCT TGGGTGGCGC ATCATATGTG 
TGGGTCCATC GGCGATATGT CCTCTTCATG AGATCCAACA AGCGGATGAA CAAGTTCCTG 
CAGAAAAATC GCTTTTTGTA TCCGGGATTC CTGGCTCTGC TGGTGTCCAG CATCTCGTTT 
CCACTGGGCA CGGGTCAGTT CCTGGCCGGC GAACTAAGTA CCCACGAGCA GGTGACACAG 
CTTTTTAGCA ATTTCACGTG GTCACGAGAT GATCTTACTG TGGAGCAGGC GGCTGTAGTG 
ACCCACTGGA TGACCAGTTA CACCAGCGTC TTTGGCAACC TTGTCATCTA CACCCTCTTT 
ACGTTTGTGG TCTCCATTAT CGCATCCACG ATACCAGTTC CGTCGGGCAT GTTCATTCCG 
GTTTTCAAGA TCGGTGCTGG CTTTGGTCGT CTGGTGGGCG AGTTCATGGC GGTGACATTT 
CCCCACGGCG TCCGGTATGG CGGTCGTCTC TCGCCCATCA TGCCCGGAGG CTATGCCGTC 
GTCGGAGCGG CCGCCTTTTC TGGCTCCGTG ACCCACACGG TGTCTGTGGC GGTGATCATT 
TTCGAGATGA CTGGTCAGAT TACGCACGTG GTACCCGTCA TGATTGCTGT GCTGGTGGCC 
AATGCCGTGG CGGCGCTGCT CCAACCGTCG ATATACGACA GTATTATATT GATTAAGAAG 
CTGCCCTACC TGCCGGATCT GCTGCCCTCC AGTTCCGGGA TGTACAGCAT ATTCGTCGAG 
GACTTTATGG TGCGGGATGT AAAGTACATA TGGCACGGCA TCTCGTATCA GAAGCTCAAA 
GAAGTCCTCA AGCTGAACAA GACGCTGAGA TCCTTGCCAC TGGTGGACAG CCCGGACAAC 
ATGATCCTGC TGGGTTCCGT GCAGCGGTAC GAGCTGATCA AAATGATCGA GAAGCACATT 
GGACGCGAGA AGCGAATGGA GGTGGCCCAG AAGTGGCAGA AGGAGGCTCA GGAAAGAGCC 
CTCGAGGAGG AGAAGAAGAA ACAGGAAGTG GAGCTCAAGA TGCGGCGACC ATCTCGATTC 
GAGGTCCTTC CAGCTCCGGA TATTCTCAGT TTACGCCAGA TTGCCAACGA CGAAATGCTG 
CCGCCCAAGA AAAGGGCGGA GACAATGCAC GGTTCGCTGG CGCCCAGGAA GTCCATTTTG 
AAGAAGACCA ACTCCTTCAA CCTGAAGACC TACGCGCAGC CCATGGGGCA CAGTCCCAGC 
ATCACGCCCT ACACCACGAT CACCGGCAAT TCCGAGTTCC GCATTCGCTC AGCCTTCGAG 
GCCATATTTA AGAAGTCCAC CACGCTCCAG GACGTACAGC CGGATCCGGA AACGGGTTCC 
CTCTCCCCGG CGGCAAGCAA CCACGAGGTG GAGGTGCCGC GAACTCCTAG CACTCCCGGT 
GTTTCCAAAA AGGTTCAGCT GCCTGCACAA AGTAATTGGG ATTTTGTAAC AGATCAAATT 
ATGCTGCAAG TAAACCCTAT TTCAACGGAA TCAAATAAAA TGCCTGCCGA GAAAGATTTC 
ACGGATAAAG CATTATCAAA TAATGGAGAT AGTTCAAAGC AATCACCGAG CATTAAATTC 
AAAGCAAATA AAGTGGCAGA TGTAAATAGA TCTCCTCAGA CGGCCAAGAG GTGCTCGAAA 
ATAAGGTTTG CTAACGAAGT TGGAGTAAAT GGTTCGCCCA CTCGAACGAA ATGTAAAATA 
AAACCAGAAA ATGTATTGGG TTACTCTATA GAGGATGTGG ATGAAACAAC AAATCCAGAA 
TCGCTGGCTG AGAGCATTCA AAAGCCGAAG TCGGTGCGAT TGCCCAGGGA ACGTGTTATT 
GACATGTCAC CGGAGGATCA GAAGCAATGG GAGCTCGAGG AAATGTTGAA GCCCATCGAT 
TTGCAGAAGG CCAATGTCCA CATCGATCCC TCGCCATTTC AGCTGGTGGA ACGCACCTCC 
ATACTCAAGG TTCACTCGTT GTTTTCCATG GTGGGTATTA ACCACGCCTA TGTCACCAAA 
ATCGGAAGAC TTGTTGGTGT CGTGGGACTC AAAGAATTGC GAAAAGCCAT CGAGGACATT 
AATAGCAATA GTTTCGTGCC CCCCACACGG GATGAGGATG CGGATGAGAA GCCGGCGGTG 
GAGAAACCTC TGCTGTCGAC GAACTCTAGT GATAAGGCCG TGGACATGAC CGTCACCTCC 
ATGGACTCGG CACTATCCAA TTCCGAGAAC TGTTCGGACA TTGAAATGGA GCACATAAAG 
CACACGGATA AGGGCACTGT ATCGCTCACC ATGCCGCCAC AGGAATCTAA GCAAAGTCCA 
TCGGCGGACA AAAGTAACAC AGAAAACGGA AATCATGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788639.1
CDS	215..3796
Translation	MVYFGDRQRDRNRDRSNQKVERIIHDEEFGEENVELVDSEWADFEKFICQLRKRRNSAMSMEEELRHVQRHPKIKSHAFYPCPPPAENARDSDSSDDDDPIGYIDTLMYGRYTKDLGEFAKDEARKLKILEKRRKQEDKQRNKELLGKHSTRAKRVSSWIWRHTVARLGEDWVFLALLGIIMALLSFIMDKGISICTNARIWLYRDLTSQPFVQYIAWVSLPVCLILFSAGFVHLIAPQSIGSGIPEMKTILRGVQLKEYLTFKTLVAKVIGLTATLGSGMPLGKEGPFVHIASIVAQLLSKLVTSFQGIYENESRNSEMLAAACAVGVGACFAAPVGGVLFSIEVTTTYFAVRNYWRGFFAAVCGATVFRLLAVWFQNADTVRALFLTNFTTEFPFDPQELFVFALIGLVCGLGGASYVWVHRRYVLFMRSNKRMNKFLQKNRFLYPGFLALLVSSISFPLGTGQFLAGELSTHEQVTQLFSNFTWSRDDLTVEQAAVVTHWMTSYTSVFGNLVIYTLFTFVVSIIASTIPVPSGMFIPVFKIGAGFGRLVGEFMAVTFPHGVRYGGRLSPIMPGGYAVVGAAAFSGSVTHTVSVAVIIFEMTGQITHVVPVMIAVLVANAVAALLQPSIYDSIILIKKLPYLPDLLPSSSGMYSIFVEDFMVRDVKYIWHGISYQKLKEVLKLNKTLRSLPLVDSPDNMILLGSVQRYELIKMIEKHIGREKRMEVAQKWQKEAQERALEEEKKKQEVELKMRRPSRFEVLPAPDILSLRQIANDEMLPPKKRAETMHGSLAPRKSILKKTNSFNLKTYAQPMGHSPSITPYTTITGNSEFRIRSAFEAIFKKSTTLQDVQPDPETGSLSPAASNHEVEVPRTPSTPGVSKKVQLPAQSNWDFVTDQIMLQVNPISTESNKMPAEKDFTDKALSNNGDSSKQSPSIKFKANKVADVNRSPQTAKRCSKIRFANEVGVNGSPTRTKCKIKPENVLGYSIEDVDETTNPESLAESIQKPKSVRLPRERVIDMSPEDQKQWELEEMLKPIDLQKANVHIDPSPFQLVERTSILKVHSLFSMVGINHAYVTKIGRLVGVVGLKELRKAIEDINSNSFVPPTRDEDADEKPAVEKPLLSTNSSDKAVDMTVTSMDSALSNSENCSDIEMEHIKHTDKGTVSLTMPPQESKQSPSADKSNTENGNHA

Target ORF information:

RefSeq Version	NM_176462.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chloride channel-a, transcript variant D (ClC-a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176462.2

ORF Insert Sequence:

ATGGTTTATT TCGGCGATCG GCAACGCGAT CGCAATCGCG ATCGGAGTAA TCAGAAAGTC 
GAGCGAATCA TTCACGATGA AGAGTTTGGC GAGGAGAACG TCGAGTTGGT GGACTCGGAG 
TGGGCGGACT TCGAGAAGTT CATTTGCCAG TTGCGCAAGC GGCGGAACAG TGCTATGTCC 
ATGGAGGAGG AACTGCGTCA CGTGCAGCGG CATCCGAAGA TCAAGTCGCA CGCCTTCTAT 
CCGTGCCCGC CGCCAGCTGA AAATGCTCGG GATTCGGACT CCTCGGACGA TGATGATCCC 
ATCGGATACA TCGATACTCT TATGTATGGT CGCTATACCA AAGATCTAGG AGAATTTGCC 
AAGGATGAAG CCAGAAAGCT CAAAATACTC GAAAAGCGAC GAAAGCAGGA AGATAAGCAA 
AGGAATAAGG AACTGCTGGG CAAACATTCG ACCCGGGCGA AGCGGGTATC ATCATGGATC 
TGGAGGCACA CGGTGGCCCG ACTGGGCGAG GATTGGGTAT TCCTGGCCCT ACTGGGCATC 
ATTATGGCCC TGCTCTCGTT CATCATGGAC AAGGGCATAT CCATATGTAC AAACGCGCGA 
ATTTGGCTGT ATCGCGATCT GACGTCACAG CCTTTTGTTC AGTACATCGC ATGGGTCTCA 
CTGCCGGTCT GTTTGATATT GTTCTCAGCC GGCTTTGTCC ATCTCATCGC ACCGCAAAGT 
ATAGGTTCTG GTATACCCGA AATGAAGACC ATACTGCGCG GCGTTCAATT GAAAGAGTAT 
CTCACATTTA AGACACTAGT GGCCAAGGTA ATTGGTTTAA CGGCAACTCT GGGCAGCGGT 
ATGCCATTAG GAAAGGAAGG TCCTTTCGTA CATATAGCAA GTATTGTAGC ACAATTATTA 
AGTAAACTTG TCACATCATT CCAAGGCATA TATGAGAATG AGTCGCGAAA CTCTGAAATG 
TTGGCGGCAG CCTGTGCTGT CGGTGTGGGC GCCTGTTTTG CCGCTCCAGT CGGTGGTGTG 
CTCTTCAGCA TAGAGGTCAC CACCACGTAC TTCGCTGTGC GTAACTACTG GCGCGGATTC 
TTTGCGGCTG TGTGCGGCGC CACTGTCTTT CGGCTCCTGG CCGTTTGGTT CCAAAACGCG 
GACACCGTCC GCGCCCTGTT CCTCACGAAC TTCACCACCG AGTTTCCCTT CGATCCTCAG 
GAGCTGTTCG TCTTTGCTTT GATTGGACTT GTGTGCGGCT TGGGTGGCGC ATCATATGTG 
TGGGTCCATC GGCGATATGT CCTCTTCATG AGATCCAACA AGCGGATGAA CAAGTTCCTG 
CAGAAAAATC GCTTTTTGTA TCCGGGATTC CTGGCTCTGC TGGTGTCCAG CATCTCGTTT 
CCACTGGGCA CGGGTCAGTT CCTGGCCGGC GAACTAAGTA CCCACGAGCA GGTGACACAG 
CTTTTTAGCA ATTTCACGTG GTCACGAGAT GATCTTACTG TGGAGCAGGC GGCTGTAGTG 
ACCCACTGGA TGACCAGTTA CACCAGCGTC TTTGGCAACC TTGTCATCTA CACCCTCTTT 
ACGTTTGTGG TCTCCATTAT CGCATCCACG ATACCAGTTC CGTCGGGCAT GTTCATTCCG 
GTTTTCAAGA TCGGTGCTGG CTTTGGTCGT CTGGTGGGCG AGTTCATGGC GGTGACATTT 
CCCCACGGCG TCCGGTATGG CGGTCGTCTC TCGCCCATCA TGCCCGGAGG CTATGCCGTC 
GTCGGAGCGG CCGCCTTTTC TGGCTCCGTG ACCCACACGG TGTCTGTGGC GGTGATCATT 
TTCGAGATGA CTGGTCAGAT TACGCACGTG GTACCCGTCA TGATTGCTGT GCTGGTGGCC 
AATGCCGTGG CGGCGCTGCT CCAACCGTCG ATATACGACA GTATTATATT GATTAAGAAG 
CTGCCCTACC TGCCGGATCT GCTGCCCTCC AGTTCCGGGA TGTACAGCAT ATTCGTCGAG 
GACTTTATGG TGCGGGATGT AAAGTACATA TGGCACGGCA TCTCGTATCA GAAGCTCAAA 
GAAGTCCTCA AGCTGAACAA GACGCTGAGA TCCTTGCCAC TGGTGGACAG CCCGGACAAC 
ATGATCCTGC TGGGTTCCGT GCAGCGGTAC GAGCTGATCA AAATGATCGA GAAGCACATT 
GGACGCGAGA AGCGAATGGA GGTGGCCCAG AAGTGGCAGA AGGAGGCTCA GGAAAGAGCC 
CTCGAGGAGG AGAAGAAGAA ACAGGAAGTG GAGCTCAAGA TGCGGCGACC ATCTCGATTC 
GAGGTCCTTC CAGCTCCGGA TATTCTCAGT TTACGCCAGA TTGCCAACGA CGAAATGCTG 
CCGCCCAAGA AAAGGGCGGA GACAATGCAC GGTTCGCTGG CGCCCAGGAA GTCCATTTTG 
AAGAAGACCA ACTCCTTCAA CCTGAAGACC TACGCGCAGC CCATGGGGCA CAGTCCCAGC 
ATCACGCCCT ACACCACGAT CACCGGCAAT TCCGAGTTCC GCATTCGCTC AGCCTTCGAG 
GCCATATTTA AGAAGTCCAC CACGCTCCAG GACGTACAGC CGGATCCGGA AACGGGTTCC 
CTCTCCCCGG CGGCAAGCAA CCACGAGGTG GAGGTGCCGC GAACTCCTAG CACTCCCGGT 
GTTTCCAAAA AGGTTCAGCT GCCTGCACAA AGTAATTGGG ATTTTGTAAC AGATCAAATT 
ATGCTGCAAG TAAACCCTAT TTCAACGGAA TCAAATAAAA TGCCTGCCGA GAAAGATTTC 
ACGGATAAAG CATTATCAAA TAATGGAGAT AGTTCAAAGC AATCACCGAG CATTAAATTC 
AAAGCAAATA AAGTGGCAGA TGTAAATAGA TCTCCTCAGA CGGCCAAGAG GTGCTCGAAA 
ATAAGGTTTG CTAACGAAGT TGGAGTAAAT GGTTCGCCCA CTCGAACGAA ATGTAAAATA 
AAACCAGAAA ATGTATTGGG TTACTCTATA GAGGATGTGG ATGAAACAAC AAATCCAGAA 
TCGCTGGCTG AGAGCATTCA AAAGCCGAAG TCGGTGCGAT TGCCCAGGGA ACGTGTTATT 
GACATGTCAC CGGAGGATCA GAAGCAATGG GAGCTCGAGG AAATGTTGAA GCCCATCGAT 
TTGCAGAAGG CCAATGTCCA CATCGATCCC TCGCCATTTC AGCTGGTGGA ACGCACCTCC 
ATACTCAAGG TTCACTCGTT GTTTTCCATG GTGGGTATTA ACCACGCCTA TGTCACCAAA 
ATCGGAAGAC TTGTTGGTGT CGTGGGACTC AAAGAATTGC GAAAAGCCAT CGAGGACATT 
AATAGCAATA GTTTCGTGCC CCCCACACGG GATGAGGATG CGGATGAGAA GCCGGCGGTG 
GAGAAACCTC TGCTGTCGAC GAACTCTAGT GATAAGGCCG TGGACATGAC CGTCACCTCC 
ATGGACTCGG CACTATCCAA TTCCGAGAAC TGTTCGGACA TTGAAATGGA GCACATAAAG 
CACACGGATA AGGGCACTGT ATCGCTCACC ATGCCGCCAC AGGAATCTAA GCAAAGTCCA 
TCGGCGGACA AAAGTAACAC AGAAAACGGA AATCATGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ClC-a ( NM_169431.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731634.2 Drosophila melanogaster chloride channel-a, transcript variant C (ClC-a), mRNA.

Price & Availability↑

CloneID	OFa04587
Clone ID Related Accession (Same CDS sequence)	NM_169431.3
Accession Version	NM_169431.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chloride channel-a, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169431.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTAACA ACAGCCACTC GCACCAGGAT GAGTACATTC GCGAATACGC CTTCGAGCCG 
GAGCTGCTGA TCAATCGCGA GGACTACCAG CGCAAGGAGG AGCGCTCGCA GAAGTCCACA 
GCCGCATCCT CCGCCTCCAC GCCTGTCCGC CAGCGCTGGG ATGAGGTCAT TGCCAAGCAG 
AAGGAGCAGC AACGCAAGCA GAGCATCGAG ATCGATCCGC CCGGCGATGA TAAGAACCAG 
ATCGAGATCG AGATCGAGGC GTTCTACTAT ATGTATGGTC GCTATACCAA AGATCTAGGA 
GAATTTGCCA AGGATGAAGC CAGAAAGCTC AAAATACTCG AAAAGCGACG AAAGCAGGAA 
GATAAGCAAA GGAATAAGGA ACTGCTGGGC AAACATTCGA CCCGGGCGAA GCGGGTATCA 
TCATGGATCT GGAGGCACAC GGTGGCCCGA CTGGGCGAGG ATTGGGTATT CCTGGCCCTA 
CTGGGCATCA TTATGGCCCT GCTCTCGTTC ATCATGGACA AGGGCATATC CATATGTACA 
AACGCGCGAA TTTGGCTGTA TCGCGATCTG ACGTCACAGC CTTTTGTTCA GTACATCGCA 
TGGGTCTCAC TGCCGGTCTG TTTGATATTG TTCTCAGCCG GCTTTGTCCA TCTCATCGCA 
CCGCAAAGTA TAGGTTCTGG TATACCCGAA ATGAAGACCA TACTGCGCGG CGTTCAATTG 
AAAGAGTATC TCACATTTAA GACACTAGTG GCCAAGGTAA TTGGTTTAAC GGCAACTCTG 
GGCAGCGGTA TGCCATTAGG AAAGGAAGGT CCTTTCGTAC ATATAGCAAG TATTGTAGCA 
CAATTATTAA GTAAACTTGT CACATCATTC CAAGGCATAT ATGAGAATGA GTCGCGAAAC 
TCTGAAATGT TGGCGGCAGC CTGTGCTGTC GGTGTGGGCG CCTGTTTTGC CGCTCCAGTC 
GGTGGTGTGC TCTTCAGCAT AGAGGTCACC ACCACGTACT TCGCTGTGCG TAACTACTGG 
CGCGGATTCT TTGCGGCTGT GTGCGGCGCC ACTGTCTTTC GGCTCCTGGC CGTTTGGTTC 
CAAAACGCGG ACACCGTCCG CGCCCTGTTC CTCACGAACT TCACCACCGA GTTTCCCTTC 
GATCCTCAGG AGCTGTTCGT CTTTGCTTTG ATTGGACTTG TGTGCGGCTT GGGTGGCGCA 
TCATATGTGT GGGTCCATCG GCGATATGTC CTCTTCATGA GATCCAACAA GCGGATGAAC 
AAGTTCCTGC AGAAAAATCG CTTTTTGTAT CCGGGATTCC TGGCTCTGCT GGTGTCCAGC 
ATCTCGTTTC CACTGGGCAC GGGTCAGTTC CTGGCCGGCG AACTAAGTAC CCACGAGCAG 
GTGACACAGC TTTTTAGCAA TTTCACGTGG TCACGAGATG ATCTTACTGT GGAGCAGGCG 
GCTGTAGTGA CCCACTGGAT GACCAGTTAC ACCAGCGTCT TTGGCAACCT TGTCATCTAC 
ACCCTCTTTA CGTTTGTGGT CTCCATTATC GCATCCACGA TACCAGTTCC GTCGGGCATG 
TTCATTCCGG TTTTCAAGAT CGGTGCTGGC TTTGGTCGTC TGGTGGGCGA GTTCATGGCG 
GTGACATTTC CCCACGGCGT CCGGTATGGC GGTCGTCTCT CGCCCATCAT GCCCGGAGGC 
TATGCCGTCG TCGGAGCGGC CGCCTTTTCT GGCTCCGTGA CCCACACGGT GTCTGTGGCG 
GTGATCATTT TCGAGATGAC TGGTCAGATT ACGCACGTGG TACCCGTCAT GATTGCTGTG 
CTGGTGGCCA ATGCCGTGGC GGCGCTGCTC CAACCGTCGA TATACGACAG TATTATATTG 
ATTAAGAAGC TGCCCTACCT GCCGGATCTG CTGCCCTCCA GTTCCGGGAT GTACAGCATA 
TTCGTCGAGG ACTTTATGGT GCGGGATGTA AAGTACATAT GGCACGGCAT CTCGTATCAG 
AAGCTCAAAG AAGTCCTCAA GCTGAACAAG ACGCTGAGAT CCTTGCCACT GGTGGACAGC 
CCGGACAACA TGATCCTGCT GGGTTCCGTG CAGCGGTACG AGCTGATCAA AATGATCGAG 
AAGCACATTG GACGCGAGAA GCGAATGGAG GTGGCCCAGA AGTGGCAGAA GGAGGCTCAG 
GAAAGAGCCC TCGAGGAGGA GAAGAAGAAA CAGGAAGTGG AGCTCAAGAT GCGGCGACCA 
TCTCGATTCG AGGTCCTTCC AGCTCCGGAT ATTCTCAGTT TACGCCAGAT TGCCAACGAC 
GAAATGCTGC CGCCCAAGAA AAGGGCGGAG ACAATGCACG GTTCGCTGGC GCCCAGGAAG 
TCCATTTTGA AGAAGACCAA CTCCTTCAAC CTGAAGACCT ACGCGCAGCC CATGGGGCAC 
AGTCCCAGCA TCACGCCCTA CACCACGATC ACCGGCAATT CCGAGTTCCG CATTCGCTCA 
GCCTTCGAGG CCATATTTAA GAAGTCCACC ACGCTCCAGG ACGTACAGCC GGATCCGGAA 
ACGGGTTCCC TCTCCCCGGC GGCAAGCAAC CACGAGGTGG AGGTGCCGCG AACTCCTAGC 
ACTCCCGGTG TTTCCAAAAA GGTTCAGCTG CCCAGGGAAC GTGTTATTGA CATGTCACCG 
GAGGATCAGA AGCAATGGGA GCTCGAGGAA ATGTTGAAGC CCATCGATTT GCAGAAGGCC 
AATGTCCACA TCGATCCCTC GCCATTTCAG CTGGTGGAAC GCACCTCCAT ACTCAAGGTT 
CACTCGTTGT TTTCCATGGT GGGTATTAAC CACGCCTATG TCACCAAAAT CGGAAGACTT 
GTTGGTGTCG TGGGACTCAA AGAATTGCGA AAAGCCATCG AGGACATTAA TAGCAATAGT 
TTCGTGCCCC CCACACGGGA TGAGGATGCG GATGAGAAGC CGGCGGTGGA GAAACCTCTG 
CTGTCGACGA ACTCTAGTGA TAAGGCCGTG GACATGACCG TCACCTCCAT GGACTCGGCA 
CTATCCAATT CCGAGAACTG TTCGGACATT GAAATGGAGC ACATAAAGCA CACGGATAAG 
GGCACTGTAT CGCTCACCAT GCCGCCACAG GAATCTAAGC AAAGTCCATC GGCGGACAAA 
AGTAACACAG AAAACGGAAA TCATGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731634.2
CDS	625..3774
Translation	MFNNSHSHQDEYIREYAFEPELLINREDYQRKEERSQKSTAASSASTPVRQRWDEVIAKQKEQQRKQSIEIDPPGDDKNQIEIEIEAFYYMYGRYTKDLGEFAKDEARKLKILEKRRKQEDKQRNKELLGKHSTRAKRVSSWIWRHTVARLGEDWVFLALLGIIMALLSFIMDKGISICTNARIWLYRDLTSQPFVQYIAWVSLPVCLILFSAGFVHLIAPQSIGSGIPEMKTILRGVQLKEYLTFKTLVAKVIGLTATLGSGMPLGKEGPFVHIASIVAQLLSKLVTSFQGIYENESRNSEMLAAACAVGVGACFAAPVGGVLFSIEVTTTYFAVRNYWRGFFAAVCGATVFRLLAVWFQNADTVRALFLTNFTTEFPFDPQELFVFALIGLVCGLGGASYVWVHRRYVLFMRSNKRMNKFLQKNRFLYPGFLALLVSSISFPLGTGQFLAGELSTHEQVTQLFSNFTWSRDDLTVEQAAVVTHWMTSYTSVFGNLVIYTLFTFVVSIIASTIPVPSGMFIPVFKIGAGFGRLVGEFMAVTFPHGVRYGGRLSPIMPGGYAVVGAAAFSGSVTHTVSVAVIIFEMTGQITHVVPVMIAVLVANAVAALLQPSIYDSIILIKKLPYLPDLLPSSSGMYSIFVEDFMVRDVKYIWHGISYQKLKEVLKLNKTLRSLPLVDSPDNMILLGSVQRYELIKMIEKHIGREKRMEVAQKWQKEAQERALEEEKKKQEVELKMRRPSRFEVLPAPDILSLRQIANDEMLPPKKRAETMHGSLAPRKSILKKTNSFNLKTYAQPMGHSPSITPYTTITGNSEFRIRSAFEAIFKKSTTLQDVQPDPETGSLSPAASNHEVEVPRTPSTPGVSKKVQLPRERVIDMSPEDQKQWELEEMLKPIDLQKANVHIDPSPFQLVERTSILKVHSLFSMVGINHAYVTKIGRLVGVVGLKELRKAIEDINSNSFVPPTRDEDADEKPAVEKPLLSTNSSDKAVDMTVTSMDSALSNSENCSDIEMEHIKHTDKGTVSLTMPPQESKQSPSADKSNTENGNHA

Target ORF information:

RefSeq Version	NM_169431.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chloride channel-a, transcript variant C (ClC-a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169431.3

ORF Insert Sequence:

ATGTTTAACA ACAGCCACTC GCACCAGGAT GAGTACATTC GCGAATACGC CTTCGAGCCG 
GAGCTGCTGA TCAATCGCGA GGACTACCAG CGCAAGGAGG AGCGCTCGCA GAAGTCCACA 
GCCGCATCCT CCGCCTCCAC GCCTGTCCGC CAGCGCTGGG ATGAGGTCAT TGCCAAGCAG 
AAGGAGCAGC AACGCAAGCA GAGCATCGAG ATCGATCCGC CCGGCGATGA TAAGAACCAG 
ATCGAGATCG AGATCGAGGC GTTCTACTAT ATGTATGGTC GCTATACCAA AGATCTAGGA 
GAATTTGCCA AGGATGAAGC CAGAAAGCTC AAAATACTCG AAAAGCGACG AAAGCAGGAA 
GATAAGCAAA GGAATAAGGA ACTGCTGGGC AAACATTCGA CCCGGGCGAA GCGGGTATCA 
TCATGGATCT GGAGGCACAC GGTGGCCCGA CTGGGCGAGG ATTGGGTATT CCTGGCCCTA 
CTGGGCATCA TTATGGCCCT GCTCTCGTTC ATCATGGACA AGGGCATATC CATATGTACA 
AACGCGCGAA TTTGGCTGTA TCGCGATCTG ACGTCACAGC CTTTTGTTCA GTACATCGCA 
TGGGTCTCAC TGCCGGTCTG TTTGATATTG TTCTCAGCCG GCTTTGTCCA TCTCATCGCA 
CCGCAAAGTA TAGGTTCTGG TATACCCGAA ATGAAGACCA TACTGCGCGG CGTTCAATTG 
AAAGAGTATC TCACATTTAA GACACTAGTG GCCAAGGTAA TTGGTTTAAC GGCAACTCTG 
GGCAGCGGTA TGCCATTAGG AAAGGAAGGT CCTTTCGTAC ATATAGCAAG TATTGTAGCA 
CAATTATTAA GTAAACTTGT CACATCATTC CAAGGCATAT ATGAGAATGA GTCGCGAAAC 
TCTGAAATGT TGGCGGCAGC CTGTGCTGTC GGTGTGGGCG CCTGTTTTGC CGCTCCAGTC 
GGTGGTGTGC TCTTCAGCAT AGAGGTCACC ACCACGTACT TCGCTGTGCG TAACTACTGG 
CGCGGATTCT TTGCGGCTGT GTGCGGCGCC ACTGTCTTTC GGCTCCTGGC CGTTTGGTTC 
CAAAACGCGG ACACCGTCCG CGCCCTGTTC CTCACGAACT TCACCACCGA GTTTCCCTTC 
GATCCTCAGG AGCTGTTCGT CTTTGCTTTG ATTGGACTTG TGTGCGGCTT GGGTGGCGCA 
TCATATGTGT GGGTCCATCG GCGATATGTC CTCTTCATGA GATCCAACAA GCGGATGAAC 
AAGTTCCTGC AGAAAAATCG CTTTTTGTAT CCGGGATTCC TGGCTCTGCT GGTGTCCAGC 
ATCTCGTTTC CACTGGGCAC GGGTCAGTTC CTGGCCGGCG AACTAAGTAC CCACGAGCAG 
GTGACACAGC TTTTTAGCAA TTTCACGTGG TCACGAGATG ATCTTACTGT GGAGCAGGCG 
GCTGTAGTGA CCCACTGGAT GACCAGTTAC ACCAGCGTCT TTGGCAACCT TGTCATCTAC 
ACCCTCTTTA CGTTTGTGGT CTCCATTATC GCATCCACGA TACCAGTTCC GTCGGGCATG 
TTCATTCCGG TTTTCAAGAT CGGTGCTGGC TTTGGTCGTC TGGTGGGCGA GTTCATGGCG 
GTGACATTTC CCCACGGCGT CCGGTATGGC GGTCGTCTCT CGCCCATCAT GCCCGGAGGC 
TATGCCGTCG TCGGAGCGGC CGCCTTTTCT GGCTCCGTGA CCCACACGGT GTCTGTGGCG 
GTGATCATTT TCGAGATGAC TGGTCAGATT ACGCACGTGG TACCCGTCAT GATTGCTGTG 
CTGGTGGCCA ATGCCGTGGC GGCGCTGCTC CAACCGTCGA TATACGACAG TATTATATTG 
ATTAAGAAGC TGCCCTACCT GCCGGATCTG CTGCCCTCCA GTTCCGGGAT GTACAGCATA 
TTCGTCGAGG ACTTTATGGT GCGGGATGTA AAGTACATAT GGCACGGCAT CTCGTATCAG 
AAGCTCAAAG AAGTCCTCAA GCTGAACAAG ACGCTGAGAT CCTTGCCACT GGTGGACAGC 
CCGGACAACA TGATCCTGCT GGGTTCCGTG CAGCGGTACG AGCTGATCAA AATGATCGAG 
AAGCACATTG GACGCGAGAA GCGAATGGAG GTGGCCCAGA AGTGGCAGAA GGAGGCTCAG 
GAAAGAGCCC TCGAGGAGGA GAAGAAGAAA CAGGAAGTGG AGCTCAAGAT GCGGCGACCA 
TCTCGATTCG AGGTCCTTCC AGCTCCGGAT ATTCTCAGTT TACGCCAGAT TGCCAACGAC 
GAAATGCTGC CGCCCAAGAA AAGGGCGGAG ACAATGCACG GTTCGCTGGC GCCCAGGAAG 
TCCATTTTGA AGAAGACCAA CTCCTTCAAC CTGAAGACCT ACGCGCAGCC CATGGGGCAC 
AGTCCCAGCA TCACGCCCTA CACCACGATC ACCGGCAATT CCGAGTTCCG CATTCGCTCA 
GCCTTCGAGG CCATATTTAA GAAGTCCACC ACGCTCCAGG ACGTACAGCC GGATCCGGAA 
ACGGGTTCCC TCTCCCCGGC GGCAAGCAAC CACGAGGTGG AGGTGCCGCG AACTCCTAGC 
ACTCCCGGTG TTTCCAAAAA GGTTCAGCTG CCCAGGGAAC GTGTTATTGA CATGTCACCG 
GAGGATCAGA AGCAATGGGA GCTCGAGGAA ATGTTGAAGC CCATCGATTT GCAGAAGGCC 
AATGTCCACA TCGATCCCTC GCCATTTCAG CTGGTGGAAC GCACCTCCAT ACTCAAGGTT 
CACTCGTTGT TTTCCATGGT GGGTATTAAC CACGCCTATG TCACCAAAAT CGGAAGACTT 
GTTGGTGTCG TGGGACTCAA AGAATTGCGA AAAGCCATCG AGGACATTAA TAGCAATAGT 
TTCGTGCCCC CCACACGGGA TGAGGATGCG GATGAGAAGC CGGCGGTGGA GAAACCTCTG 
CTGTCGACGA ACTCTAGTGA TAAGGCCGTG GACATGACCG TCACCTCCAT GGACTCGGCA 
CTATCCAATT CCGAGAACTG TTCGGACATT GAAATGGAGC ACATAAAGCA CACGGATAAG 
GGCACTGTAT CGCTCACCAT GCCGCCACAG GAATCTAAGC AAAGTCCATC GGCGGACAAA 
AGTAACACAG AAAACGGAAA TCATGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ClC-a ( NM_169432.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731635.2 Drosophila melanogaster chloride channel-a, transcript variant A (ClC-a), mRNA.

Price & Availability↑

CloneID	OFa04588
Clone ID Related Accession (Same CDS sequence)	NM_169432.3
Accession Version	NM_169432.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chloride channel-a, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169432.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTAACA ACAGCCACTC GCACCAGGAT GAGTACATTC GCGAATACGC CTTCGAGCCG 
GAGCTGCTGA TCAATCGCGA GGACTACCAG CGCAAGGAGG AGCGCTCGCA GAAGTCCACA 
GCCGCATCCT CCGCCTCCAC GCCTGTCCGC CAGCGCTGGG ATGAGGTCAT TGCCAAGCAG 
AAGGAGCAGC AACGCAAGCA GAGCATCGAG ATCGATCCGC CCGGCGATGA TAAGAACCAG 
ATCGAGATCG AGATCGAGGC GTTCTACTAT ATGTATGGTC GCTATACCAA AGATCTAGGA 
GAATTTGCCA AGGATGAAGC CAGAAAGCTC AAAATACTCG AAAAGCGACG AAAGCAGGAA 
GATAAGCAAA GGAATAAGGA ACTGCTGGGC AAACATTCGA CCCGGGCGAA GCGGGTATCA 
TCATGGATCT GGAGGCACAC GGTGGCCCGA CTGGGCGAGG ATTGGGTATT CCTGGCCCTA 
CTGGGCATCA TTATGGCCCT GCTCTCGTTC ATCATGGACA AGGGCATATC CATATGTACA 
AACGCGCGAA TTTGGCTGTA TCGCGATCTG ACGTCACAGC CTTTTGTTCA GTACATCGCA 
TGGGTCTCAC TGCCGGTCTG TTTGATATTG TTCTCAGCCG GCTTTGTCCA TCTCATCGCA 
CCGCAAAGTA TAGGTTCTGG TATACCCGAA ATGAAGACCA TACTGCGCGG CGTTCAATTG 
AAAGAGTATC TCACATTTAA GACACTAGTG GCCAAGGTAA TTGGTTTAAC GGCAACTCTG 
GGCAGCGGTA TGCCATTAGG AAAGGAAGGT CCTTTCGTAC ATATAGCAAG TATTGTAGCA 
CAATTATTAA GTAAACTTGT CACATCATTC CAAGGCATAT ATGAGAATGA GTCGCGAAAC 
TCTGAAATGT TGGCGGCAGC CTGTGCTGTC GGTGTGGGCG CCTGTTTTGC CGCTCCAGTC 
GGTGGTGTGC TCTTCAGCAT AGAGGTCACC ACCACGTACT TCGCTGTGCG TAACTACTGG 
CGCGGATTCT TTGCGGCTGT GTGCGGCGCC ACTGTCTTTC GGCTCCTGGC CGTTTGGTTC 
CAAAACGCGG ACACCGTCCG CGCCCTGTTC CTCACGAACT TCACCACCGA GTTTCCCTTC 
GATCCTCAGG AGCTGTTCGT CTTTGCTTTG ATTGGACTTG TGTGCGGCTT GGGTGGCGCA 
TCATATGTGT GGGTCCATCG GCGATATGTC CTCTTCATGA GATCCAACAA GCGGATGAAC 
AAGTTCCTGC AGAAAAATCG CTTTTTGTAT CCGGGATTCC TGGCTCTGCT GGTGTCCAGC 
ATCTCGTTTC CACTGGGCAC GGGTCAGTTC CTGGCCGGCG AACTAAGTAC CCACGAGCAG 
GTGACACAGC TTTTTAGCAA TTTCACGTGG TCACGAGATG ATCTTACTGT GGAGCAGGCG 
GCTGTAGTGA CCCACTGGAT GACCAGTTAC ACCAGCGTCT TTGGCAACCT TGTCATCTAC 
ACCCTCTTTA CGTTTGTGGT CTCCATTATC GCATCCACGA TACCAGTTCC GTCGGGCATG 
TTCATTCCGG TTTTCAAGAT CGGTGCTGGC TTTGGTCGTC TGGTGGGCGA GTTCATGGCG 
GTGACATTTC CCCACGGCGT CCGGTATGGC GGTCGTCTCT CGCCCATCAT GCCCGGAGGC 
TATGCCGTCG TCGGAGCGGC CGCCTTTTCT GGCTCCGTGA CCCACACGGT GTCTGTGGCG 
GTGATCATTT TCGAGATGAC TGGTCAGATT ACGCACGTGG TACCCGTCAT GATTGCTGTG 
CTGGTGGCCA ATGCCGTGGC GGCGCTGCTC CAACCGTCGA TATACGACAG TATTATATTG 
ATTAAGAAGC TGCCCTACCT GCCGGATCTG CTGCCCTCCA GTTCCGGGAT GTACAGCATA 
TTCGTCGAGG ACTTTATGGT GCGGGATGTA AAGTACATAT GGCACGGCAT CTCGTATCAG 
AAGCTCAAAG AAGTCCTCAA GCTGAACAAG ACGCTGAGAT CCTTGCCACT GGTGGACAGC 
CCGGACAACA TGATCCTGCT GGGTTCCGTG CAGCGGTACG AGCTGATCAA AATGATCGAG 
AAGCACATTG GACGCGAGAA GCGAATGGAG GTGGCCCAGA AGTGGCAGAA GGAGGCTCAG 
GAAAGAGCCC TCGAGGAGGA GAAGAAGAAA CAGGAAGTGG AGCTCAAGAT GCGGCGACCA 
TCTCGATTCG AGGTCCTTCC AGCTCCGGAT ATTCTCAGTT TACGCCAGAT TGCCAACGAC 
GAAATGCTGC CGCCCAAGAA AAGGGCGGAG ACAATGCACG GTTCGCTGGC GCCCAGGAAG 
TCCATTTTGA AGAAGACCAA CTCCTTCAAC CTGAAGACCT ACGCGCAGCC CATGGGGCAC 
AGTCCCAGCA TCACGCCCTA CACCACGATC ACCGGCAATT CCGAGTTCCG CATTCGCTCA 
GCCTTCGAGG CCATATTTAA GAAGTCCACC ACGCTCCAGG ACGTACAGCC GGATCCGGAA 
ACGGGTTCCC TCTCCCCGGC GGCAAGCAAC CACGAGGTGG AGGTGCCGCG AACTCCTAGC 
ACTCCCGGTG TTTCCAAAAA GGTTCAGCTG CCTGCACAAA GTAATTGGGA TTTTGTAACA 
GATCAAATTA TGCTGCAAGT AAACCCTATT TCAACGGAAT CAAATAAAAT GCCTGCCGAG 
AAAGATTTCA CGGATAAAGC ATTATCAAAT AATGGAGATA GTTCAAAGCA ATCACCGAGC 
ATTAAATTCA AAGCAAATAA AGTGGCAGAT GTAAATAGAT CTCCTCAGAC GGCCAAGAGG 
TGCTCGAAAA TAAGGTTTGC TAACGAAGTT GGAGTAAATG GTTCGCCCAC TCGAACGAAA 
TGTAAAATAA AACCAGAAAA TGTATTGGGT TACTCTATAG AGGATGTGGA TGAAACAACA 
AATCCAGAAT CGCTGGCTGA GAGCATTCAA AAGCCGAAGT CGGTGCGATT GCCCAGGGAA 
CGTGTTATTG ACATGTCACC GGAGGATCAG AAGCAATGGG AGCTCGAGGA AATGTTGAAG 
CCCATCGATT TGCAGAAGGC CAATGTCCAC ATCGATCCCT CGCCATTTCA GCTGGTGGAA 
CGCACCTCCA TACTCAAGGT TCACTCGTTG TTTTCCATGG TGGGTATTAA CCACGCCTAT 
GTCACCAAAA TCGGAAGACT TGTTGGTGTC GTGGGACTCA AAGAATTGCG AAAAGCCATC 
GAGGACATTA ATAGCAATAG TTTCGTGCCC CCCACACGGG ATGAGGATGC GGATGAGAAG 
CCGGCGGTGG AGAAACCTCT GCTGTCGACG AACTCTAGTG ATAAGGCCGT GGACATGACC 
GTCACCTCCA TGGACTCGGC ACTATCCAAT TCCGAGAACT GTTCGGACAT TGAAATGGAG 
CACATAAAGC ACACGGATAA GGGCACTGTA TCGCTCACCA TGCCGCCACA GGAATCTAAG 
CAAAGTCCAT CGGCGGACAA AAGTAACACA GAAAACGGAA ATCATGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731635.2
CDS	625..4155
Translation	MFNNSHSHQDEYIREYAFEPELLINREDYQRKEERSQKSTAASSASTPVRQRWDEVIAKQKEQQRKQSIEIDPPGDDKNQIEIEIEAFYYMYGRYTKDLGEFAKDEARKLKILEKRRKQEDKQRNKELLGKHSTRAKRVSSWIWRHTVARLGEDWVFLALLGIIMALLSFIMDKGISICTNARIWLYRDLTSQPFVQYIAWVSLPVCLILFSAGFVHLIAPQSIGSGIPEMKTILRGVQLKEYLTFKTLVAKVIGLTATLGSGMPLGKEGPFVHIASIVAQLLSKLVTSFQGIYENESRNSEMLAAACAVGVGACFAAPVGGVLFSIEVTTTYFAVRNYWRGFFAAVCGATVFRLLAVWFQNADTVRALFLTNFTTEFPFDPQELFVFALIGLVCGLGGASYVWVHRRYVLFMRSNKRMNKFLQKNRFLYPGFLALLVSSISFPLGTGQFLAGELSTHEQVTQLFSNFTWSRDDLTVEQAAVVTHWMTSYTSVFGNLVIYTLFTFVVSIIASTIPVPSGMFIPVFKIGAGFGRLVGEFMAVTFPHGVRYGGRLSPIMPGGYAVVGAAAFSGSVTHTVSVAVIIFEMTGQITHVVPVMIAVLVANAVAALLQPSIYDSIILIKKLPYLPDLLPSSSGMYSIFVEDFMVRDVKYIWHGISYQKLKEVLKLNKTLRSLPLVDSPDNMILLGSVQRYELIKMIEKHIGREKRMEVAQKWQKEAQERALEEEKKKQEVELKMRRPSRFEVLPAPDILSLRQIANDEMLPPKKRAETMHGSLAPRKSILKKTNSFNLKTYAQPMGHSPSITPYTTITGNSEFRIRSAFEAIFKKSTTLQDVQPDPETGSLSPAASNHEVEVPRTPSTPGVSKKVQLPAQSNWDFVTDQIMLQVNPISTESNKMPAEKDFTDKALSNNGDSSKQSPSIKFKANKVADVNRSPQTAKRCSKIRFANEVGVNGSPTRTKCKIKPENVLGYSIEDVDETTNPESLAESIQKPKSVRLPRERVIDMSPEDQKQWELEEMLKPIDLQKANVHIDPSPFQLVERTSILKVHSLFSMVGINHAYVTKIGRLVGVVGLKELRKAIEDINSNSFVPPTRDEDADEKPAVEKPLLSTNSSDKAVDMTVTSMDSALSNSENCSDIEMEHIKHTDKGTVSLTMPPQESKQSPSADKSNTENGNHA

Target ORF information:

RefSeq Version	NM_169432.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chloride channel-a, transcript variant A (ClC-a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169432.3

ORF Insert Sequence:

ATGTTTAACA ACAGCCACTC GCACCAGGAT GAGTACATTC GCGAATACGC CTTCGAGCCG 
GAGCTGCTGA TCAATCGCGA GGACTACCAG CGCAAGGAGG AGCGCTCGCA GAAGTCCACA 
GCCGCATCCT CCGCCTCCAC GCCTGTCCGC CAGCGCTGGG ATGAGGTCAT TGCCAAGCAG 
AAGGAGCAGC AACGCAAGCA GAGCATCGAG ATCGATCCGC CCGGCGATGA TAAGAACCAG 
ATCGAGATCG AGATCGAGGC GTTCTACTAT ATGTATGGTC GCTATACCAA AGATCTAGGA 
GAATTTGCCA AGGATGAAGC CAGAAAGCTC AAAATACTCG AAAAGCGACG AAAGCAGGAA 
GATAAGCAAA GGAATAAGGA ACTGCTGGGC AAACATTCGA CCCGGGCGAA GCGGGTATCA 
TCATGGATCT GGAGGCACAC GGTGGCCCGA CTGGGCGAGG ATTGGGTATT CCTGGCCCTA 
CTGGGCATCA TTATGGCCCT GCTCTCGTTC ATCATGGACA AGGGCATATC CATATGTACA 
AACGCGCGAA TTTGGCTGTA TCGCGATCTG ACGTCACAGC CTTTTGTTCA GTACATCGCA 
TGGGTCTCAC TGCCGGTCTG TTTGATATTG TTCTCAGCCG GCTTTGTCCA TCTCATCGCA 
CCGCAAAGTA TAGGTTCTGG TATACCCGAA ATGAAGACCA TACTGCGCGG CGTTCAATTG 
AAAGAGTATC TCACATTTAA GACACTAGTG GCCAAGGTAA TTGGTTTAAC GGCAACTCTG 
GGCAGCGGTA TGCCATTAGG AAAGGAAGGT CCTTTCGTAC ATATAGCAAG TATTGTAGCA 
CAATTATTAA GTAAACTTGT CACATCATTC CAAGGCATAT ATGAGAATGA GTCGCGAAAC 
TCTGAAATGT TGGCGGCAGC CTGTGCTGTC GGTGTGGGCG CCTGTTTTGC CGCTCCAGTC 
GGTGGTGTGC TCTTCAGCAT AGAGGTCACC ACCACGTACT TCGCTGTGCG TAACTACTGG 
CGCGGATTCT TTGCGGCTGT GTGCGGCGCC ACTGTCTTTC GGCTCCTGGC CGTTTGGTTC 
CAAAACGCGG ACACCGTCCG CGCCCTGTTC CTCACGAACT TCACCACCGA GTTTCCCTTC 
GATCCTCAGG AGCTGTTCGT CTTTGCTTTG ATTGGACTTG TGTGCGGCTT GGGTGGCGCA 
TCATATGTGT GGGTCCATCG GCGATATGTC CTCTTCATGA GATCCAACAA GCGGATGAAC 
AAGTTCCTGC AGAAAAATCG CTTTTTGTAT CCGGGATTCC TGGCTCTGCT GGTGTCCAGC 
ATCTCGTTTC CACTGGGCAC GGGTCAGTTC CTGGCCGGCG AACTAAGTAC CCACGAGCAG 
GTGACACAGC TTTTTAGCAA TTTCACGTGG TCACGAGATG ATCTTACTGT GGAGCAGGCG 
GCTGTAGTGA CCCACTGGAT GACCAGTTAC ACCAGCGTCT TTGGCAACCT TGTCATCTAC 
ACCCTCTTTA CGTTTGTGGT CTCCATTATC GCATCCACGA TACCAGTTCC GTCGGGCATG 
TTCATTCCGG TTTTCAAGAT CGGTGCTGGC TTTGGTCGTC TGGTGGGCGA GTTCATGGCG 
GTGACATTTC CCCACGGCGT CCGGTATGGC GGTCGTCTCT CGCCCATCAT GCCCGGAGGC 
TATGCCGTCG TCGGAGCGGC CGCCTTTTCT GGCTCCGTGA CCCACACGGT GTCTGTGGCG 
GTGATCATTT TCGAGATGAC TGGTCAGATT ACGCACGTGG TACCCGTCAT GATTGCTGTG 
CTGGTGGCCA ATGCCGTGGC GGCGCTGCTC CAACCGTCGA TATACGACAG TATTATATTG 
ATTAAGAAGC TGCCCTACCT GCCGGATCTG CTGCCCTCCA GTTCCGGGAT GTACAGCATA 
TTCGTCGAGG ACTTTATGGT GCGGGATGTA AAGTACATAT GGCACGGCAT CTCGTATCAG 
AAGCTCAAAG AAGTCCTCAA GCTGAACAAG ACGCTGAGAT CCTTGCCACT GGTGGACAGC 
CCGGACAACA TGATCCTGCT GGGTTCCGTG CAGCGGTACG AGCTGATCAA AATGATCGAG 
AAGCACATTG GACGCGAGAA GCGAATGGAG GTGGCCCAGA AGTGGCAGAA GGAGGCTCAG 
GAAAGAGCCC TCGAGGAGGA GAAGAAGAAA CAGGAAGTGG AGCTCAAGAT GCGGCGACCA 
TCTCGATTCG AGGTCCTTCC AGCTCCGGAT ATTCTCAGTT TACGCCAGAT TGCCAACGAC 
GAAATGCTGC CGCCCAAGAA AAGGGCGGAG ACAATGCACG GTTCGCTGGC GCCCAGGAAG 
TCCATTTTGA AGAAGACCAA CTCCTTCAAC CTGAAGACCT ACGCGCAGCC CATGGGGCAC 
AGTCCCAGCA TCACGCCCTA CACCACGATC ACCGGCAATT CCGAGTTCCG CATTCGCTCA 
GCCTTCGAGG CCATATTTAA GAAGTCCACC ACGCTCCAGG ACGTACAGCC GGATCCGGAA 
ACGGGTTCCC TCTCCCCGGC GGCAAGCAAC CACGAGGTGG AGGTGCCGCG AACTCCTAGC 
ACTCCCGGTG TTTCCAAAAA GGTTCAGCTG CCTGCACAAA GTAATTGGGA TTTTGTAACA 
GATCAAATTA TGCTGCAAGT AAACCCTATT TCAACGGAAT CAAATAAAAT GCCTGCCGAG 
AAAGATTTCA CGGATAAAGC ATTATCAAAT AATGGAGATA GTTCAAAGCA ATCACCGAGC 
ATTAAATTCA AAGCAAATAA AGTGGCAGAT GTAAATAGAT CTCCTCAGAC GGCCAAGAGG 
TGCTCGAAAA TAAGGTTTGC TAACGAAGTT GGAGTAAATG GTTCGCCCAC TCGAACGAAA 
TGTAAAATAA AACCAGAAAA TGTATTGGGT TACTCTATAG AGGATGTGGA TGAAACAACA 
AATCCAGAAT CGCTGGCTGA GAGCATTCAA AAGCCGAAGT CGGTGCGATT GCCCAGGGAA 
CGTGTTATTG ACATGTCACC GGAGGATCAG AAGCAATGGG AGCTCGAGGA AATGTTGAAG 
CCCATCGATT TGCAGAAGGC CAATGTCCAC ATCGATCCCT CGCCATTTCA GCTGGTGGAA 
CGCACCTCCA TACTCAAGGT TCACTCGTTG TTTTCCATGG TGGGTATTAA CCACGCCTAT 
GTCACCAAAA TCGGAAGACT TGTTGGTGTC GTGGGACTCA AAGAATTGCG AAAAGCCATC 
GAGGACATTA ATAGCAATAG TTTCGTGCCC CCCACACGGG ATGAGGATGC GGATGAGAAG 
CCGGCGGTGG AGAAACCTCT GCTGTCGACG AACTCTAGTG ATAAGGCCGT GGACATGACC 
GTCACCTCCA TGGACTCGGC ACTATCCAAT TCCGAGAACT GTTCGGACAT TGAAATGGAG 
CACATAAAGC ACACGGATAA GGGCACTGTA TCGCTCACCA TGCCGCCACA GGAATCTAAG 
CAAAGTCCAT CGGCGGACAA AAGTAACACA GAAAACGGAA ATCATGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ClC-a ( NM_001170121.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163592.1 Drosophila melanogaster chloride channel-a, transcript variant H (ClC-a), mRNA.

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CloneID	OFa07780
Clone ID Related Accession (Same CDS sequence)	NM_001170121.1
Accession Version	NM_001170121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	chloride channel-a, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAGCGA CCAGGAACGG CGGCCAGCTG CTCATCGCTC CTTCGCCGCA GACTGCTGCC 
CGTCTGCTGC CCCTGCGCAC CTACTCCAAC GCCTCCTCGC TGGGCCACCA CCATGACAGG 
AGCACCCTGT CCGACGGCGA CGAGGAGGAG GGCGGCCTTG GCTACACCCA CACGCTGATG 
TATGGTCGCT ATACCAAAGA TCTAGGAGAA TTTGCCAAGG ATGAAGCCAG AAAGCTCAAA 
ATACTCGAAA AGCGACGAAA GCAGGAAGAT AAGCAAAGGA ATAAGGAACT GCTGGGCAAA 
CATTCGACCC GGGCGAAGCG GGTATCATCA TGGATCTGGA GGCACACGGT GGCCCGACTG 
GGCGAGGATT GGGTATTCCT GGCCCTACTG GGCATCATTA TGGCCCTGCT CTCGTTCATC 
ATGGACAAGG GCATATCCAT ATGTACAAAC GCGCGAATTT GGCTGTATCG CGATCTGACG 
TCACAGCCTT TTGTTCAGTA CATCGCATGG GTCTCACTGC CGGTCTGTTT GATATTGTTC 
TCAGCCGGCT TTGTCCATCT CATCGCACCG CAAAGTATAG GTTCTGGTAT ACCCGAAATG 
AAGACCATAC TGCGCGGCGT TCAATTGAAA GAGTATCTCA CATTTAAGAC ACTAGTGGCC 
AAGGTAATTG GTTTAACGGC AACTCTGGGC AGCGGTATGC CATTAGGAAA GGAAGGTCCT 
TTCGTACATA TAGCAAGTAT TGTAGCACAA TTATTAAGTA AACTTGTCAC ATCATTCCAA 
GGCATATATG AGAATGAGTC GCGAAACTCT GAAATGTTGG CGGCAGCCTG TGCTGTCGGT 
GTGGGCGCCT GTTTTGCCGC TCCAGTCGGT GGTGTGCTCT TCAGCATAGA GGTCACCACC 
ACGTACTTCG CTGTGCGTAA CTACTGGCGC GGATTCTTTG CGGCTGTGTG CGGCGCCACT 
GTCTTTCGGC TCCTGGCCGT TTGGTTCCAA AACGCGGACA CCGTCCGCGC CCTGTTCCTC 
ACGAACTTCA CCACCGAGTT TCCCTTCGAT CCTCAGGAGC TGTTCGTCTT TGCTTTGATT 
GGACTTGTGT GCGGCTTGGG TGGCGCATCA TATGTGTGGG TCCATCGGCG ATATGTCCTC 
TTCATGAGAT CCAACAAGCG GATGAACAAG TTCCTGCAGA AAAATCGCTT TTTGTATCCG 
GGATTCCTGG CTCTGCTGGT GTCCAGCATC TCGTTTCCAC TGGGCACGGG TCAGTTCCTG 
GCCGGCGAAC TAAGTACCCA CGAGCAGGTG ACACAGCTTT TTAGCAATTT CACGTGGTCA 
CGAGATGATC TTACTGTGGA GCAGGCGGCT GTAGTGACCC ACTGGATGAC CAGTTACACC 
AGCGTCTTTG GCAACCTTGT CATCTACACC CTCTTTACGT TTGTGGTCTC CATTATCGCA 
TCCACGATAC CAGTTCCGTC GGGCATGTTC ATTCCGGTTT TCAAGATCGG TGCTGGCTTT 
GGTCGTCTGG TGGGCGAGTT CATGGCGGTG ACATTTCCCC ACGGCGTCCG GTATGGCGGT 
CGTCTCTCGC CCATCATGCC CGGAGGCTAT GCCGTCGTCG GAGCGGCCGC CTTTTCTGGC 
TCCGTGACCC ACACGGTGTC TGTGGCGGTG ATCATTTTCG AGATGACTGG TCAGATTACG 
CACGTGGTAC CCGTCATGAT TGCTGTGCTG GTGGCCAATG CCGTGGCGGC GCTGCTCCAA 
CCGTCGATAT ACGACAGTAT TATATTGATT AAGAAGCTGC CCTACCTGCC GGATCTGCTG 
CCCTCCAGTT CCGGGATGTA CAGCATATTC GTCGAGGACT TTATGGTGCG GGATGTAAAG 
TACATATGGC ACGGCATCTC GTATCAGAAG CTCAAAGAAG TCCTCAAGCT GAACAAGACG 
CTGAGATCCT TGCCACTGGT GGACAGCCCG GACAACATGA TCCTGCTGGG TTCCGTGCAG 
CGGTACGAGC TGATCAAAAT GATCGAGAAG CACATTGGAC GCGAGAAGCG AATGGAGGTG 
GCCCAGAAGT GGCAGAAGGA GGCTCAGGAA AGAGCCCTCG AGGAGGAGAA GAAGAAACAG 
GAAGTGGAGC TCAAGATGCG GCGACCATCT CGATTCGAGG TCCTTCCAGC TCCGGATATT 
CTCAGTTTAC GCCAGATTGC CAACGACGAA ATGCTGCCGC CCAAGAAAAG GGCGGAGACA 
ATGCACGGTT CGCTGGCGCC CAGGAAGTCC ATTTTGAAGA AGACCAACTC CTTCAACCTG 
AAGACCTACG CGCAGCCCAT GGGGCACAGT CCCAGCATCA CGCCCTACAC CACGATCACC 
GGCAATTCCG AGTTCCGCAT TCGCTCAGCC TTCGAGGCCA TATTTAAGAA GTCCACCACG 
CTCCAGGACG TACAGCCGGA TCCGGAAACG GGTTCCCTCT CCCCGGCGGC AAGCAACCAC 
GAGGTGGAGG TGCCGCGAAC TCCTAGCACT CCCGGTGTTT CCAAAAAGGT TCAGCTGCCT 
GCACAAAGTA ATTGGGATTT TGTAACAGAT CAAATTATGC TGCAAGTAAA CCCTATTTCA 
ACGGAATCAA ATAAAATGCC TGCCGAGAAA GATTTCACGG ATAAAGCATT ATCAAATAAT 
GGAGATAGTT CAAAGCAATC ACCGAGCATT AAATTCAAAG CAAATAAAGT GGCAGATGTA 
AATAGATCTC CTCAGACGGC CAAGAGGTGC TCGAAAATAA GGTTTGCTAA CGAAGTTGGA 
GTAAATGGTT CGCCCACTCG AACGAAATGT AAAATAAAAC CAGAAAATGT ATTGGGTTAC 
TCTATAGAGG ATGTGGATGA AACAACAAAT CCAGAATCGC TGGCTGAGAG CATTCAAAAG 
CCGAAGTCGG TGCGATTGCC CAGGGAACGT GTTATTGACA TGTCACCGGA GGATCAGAAG 
CAATGGGAGC TCGAGGAAAT GTTGAAGCCC ATCGATTTGC AGAAGGCCAA TGTCCACATC 
GATCCCTCGC CATTTCAGCT GGTGGAACGC ACCTCCATAC TCAAGGTTCA CTCGTTGTTT 
TCCATGGTGG GTATTAACCA CGCCTATGTC ACCAAAATCG GAAGACTTGT TGGTGTCGTG 
GGACTCAAAG AATTGCGAAA AGCCATCGAG GACATTAATA GCAATAGTTT CGTGCCCCCC 
ACACGGGATG AGGATGCGGA TGAGAAGCCG GCGGTGGAGA AACCTCTGCT GTCGACGAAC 
TCTAGTGATA AGGCCGTGGA CATGACCGTC ACCTCCATGG ACTCGGCACT ATCCAATTCC 
GAGAACTGTT CGGACATTGA AATGGAGCAC ATAAAGCACA CGGATAAGGG CACTGTATCG 
CTCACCATGC CGCCACAGGA ATCTAAGCAA AGTCCATCGG CGGACAAAAG TAACACAGAA 
AACGGAAATC ATGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163592.1
CDS	809..4246
Translation	MKATRNGGQLLIAPSPQTAARLLPLRTYSNASSLGHHHDRSTLSDGDEEEGGLGYTHTLMYGRYTKDLGEFAKDEARKLKILEKRRKQEDKQRNKELLGKHSTRAKRVSSWIWRHTVARLGEDWVFLALLGIIMALLSFIMDKGISICTNARIWLYRDLTSQPFVQYIAWVSLPVCLILFSAGFVHLIAPQSIGSGIPEMKTILRGVQLKEYLTFKTLVAKVIGLTATLGSGMPLGKEGPFVHIASIVAQLLSKLVTSFQGIYENESRNSEMLAAACAVGVGACFAAPVGGVLFSIEVTTTYFAVRNYWRGFFAAVCGATVFRLLAVWFQNADTVRALFLTNFTTEFPFDPQELFVFALIGLVCGLGGASYVWVHRRYVLFMRSNKRMNKFLQKNRFLYPGFLALLVSSISFPLGTGQFLAGELSTHEQVTQLFSNFTWSRDDLTVEQAAVVTHWMTSYTSVFGNLVIYTLFTFVVSIIASTIPVPSGMFIPVFKIGAGFGRLVGEFMAVTFPHGVRYGGRLSPIMPGGYAVVGAAAFSGSVTHTVSVAVIIFEMTGQITHVVPVMIAVLVANAVAALLQPSIYDSIILIKKLPYLPDLLPSSSGMYSIFVEDFMVRDVKYIWHGISYQKLKEVLKLNKTLRSLPLVDSPDNMILLGSVQRYELIKMIEKHIGREKRMEVAQKWQKEAQERALEEEKKKQEVELKMRRPSRFEVLPAPDILSLRQIANDEMLPPKKRAETMHGSLAPRKSILKKTNSFNLKTYAQPMGHSPSITPYTTITGNSEFRIRSAFEAIFKKSTTLQDVQPDPETGSLSPAASNHEVEVPRTPSTPGVSKKVQLPAQSNWDFVTDQIMLQVNPISTESNKMPAEKDFTDKALSNNGDSSKQSPSIKFKANKVADVNRSPQTAKRCSKIRFANEVGVNGSPTRTKCKIKPENVLGYSIEDVDETTNPESLAESIQKPKSVRLPRERVIDMSPEDQKQWELEEMLKPIDLQKANVHIDPSPFQLVERTSILKVHSLFSMVGINHAYVTKIGRLVGVVGLKELRKAIEDINSNSFVPPTRDEDADEKPAVEKPLLSTNSSDKAVDMTVTSMDSALSNSENCSDIEMEHIKHTDKGTVSLTMPPQESKQSPSADKSNTENGNHA

Target ORF information:

RefSeq Version	NM_001170121.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster chloride channel-a, transcript variant H (ClC-a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170121.1

ORF Insert Sequence:

ATGAAAGCGA CCAGGAACGG CGGCCAGCTG CTCATCGCTC CTTCGCCGCA GACTGCTGCC 
CGTCTGCTGC CCCTGCGCAC CTACTCCAAC GCCTCCTCGC TGGGCCACCA CCATGACAGG 
AGCACCCTGT CCGACGGCGA CGAGGAGGAG GGCGGCCTTG GCTACACCCA CACGCTGATG 
TATGGTCGCT ATACCAAAGA TCTAGGAGAA TTTGCCAAGG ATGAAGCCAG AAAGCTCAAA 
ATACTCGAAA AGCGACGAAA GCAGGAAGAT AAGCAAAGGA ATAAGGAACT GCTGGGCAAA 
CATTCGACCC GGGCGAAGCG GGTATCATCA TGGATCTGGA GGCACACGGT GGCCCGACTG 
GGCGAGGATT GGGTATTCCT GGCCCTACTG GGCATCATTA TGGCCCTGCT CTCGTTCATC 
ATGGACAAGG GCATATCCAT ATGTACAAAC GCGCGAATTT GGCTGTATCG CGATCTGACG 
TCACAGCCTT TTGTTCAGTA CATCGCATGG GTCTCACTGC CGGTCTGTTT GATATTGTTC 
TCAGCCGGCT TTGTCCATCT CATCGCACCG CAAAGTATAG GTTCTGGTAT ACCCGAAATG 
AAGACCATAC TGCGCGGCGT TCAATTGAAA GAGTATCTCA CATTTAAGAC ACTAGTGGCC 
AAGGTAATTG GTTTAACGGC AACTCTGGGC AGCGGTATGC CATTAGGAAA GGAAGGTCCT 
TTCGTACATA TAGCAAGTAT TGTAGCACAA TTATTAAGTA AACTTGTCAC ATCATTCCAA 
GGCATATATG AGAATGAGTC GCGAAACTCT GAAATGTTGG CGGCAGCCTG TGCTGTCGGT 
GTGGGCGCCT GTTTTGCCGC TCCAGTCGGT GGTGTGCTCT TCAGCATAGA GGTCACCACC 
ACGTACTTCG CTGTGCGTAA CTACTGGCGC GGATTCTTTG CGGCTGTGTG CGGCGCCACT 
GTCTTTCGGC TCCTGGCCGT TTGGTTCCAA AACGCGGACA CCGTCCGCGC CCTGTTCCTC 
ACGAACTTCA CCACCGAGTT TCCCTTCGAT CCTCAGGAGC TGTTCGTCTT TGCTTTGATT 
GGACTTGTGT GCGGCTTGGG TGGCGCATCA TATGTGTGGG TCCATCGGCG ATATGTCCTC 
TTCATGAGAT CCAACAAGCG GATGAACAAG TTCCTGCAGA AAAATCGCTT TTTGTATCCG 
GGATTCCTGG CTCTGCTGGT GTCCAGCATC TCGTTTCCAC TGGGCACGGG TCAGTTCCTG 
GCCGGCGAAC TAAGTACCCA CGAGCAGGTG ACACAGCTTT TTAGCAATTT CACGTGGTCA 
CGAGATGATC TTACTGTGGA GCAGGCGGCT GTAGTGACCC ACTGGATGAC CAGTTACACC 
AGCGTCTTTG GCAACCTTGT CATCTACACC CTCTTTACGT TTGTGGTCTC CATTATCGCA 
TCCACGATAC CAGTTCCGTC GGGCATGTTC ATTCCGGTTT TCAAGATCGG TGCTGGCTTT 
GGTCGTCTGG TGGGCGAGTT CATGGCGGTG ACATTTCCCC ACGGCGTCCG GTATGGCGGT 
CGTCTCTCGC CCATCATGCC CGGAGGCTAT GCCGTCGTCG GAGCGGCCGC CTTTTCTGGC 
TCCGTGACCC ACACGGTGTC TGTGGCGGTG ATCATTTTCG AGATGACTGG TCAGATTACG 
CACGTGGTAC CCGTCATGAT TGCTGTGCTG GTGGCCAATG CCGTGGCGGC GCTGCTCCAA 
CCGTCGATAT ACGACAGTAT TATATTGATT AAGAAGCTGC CCTACCTGCC GGATCTGCTG 
CCCTCCAGTT CCGGGATGTA CAGCATATTC GTCGAGGACT TTATGGTGCG GGATGTAAAG 
TACATATGGC ACGGCATCTC GTATCAGAAG CTCAAAGAAG TCCTCAAGCT GAACAAGACG 
CTGAGATCCT TGCCACTGGT GGACAGCCCG GACAACATGA TCCTGCTGGG TTCCGTGCAG 
CGGTACGAGC TGATCAAAAT GATCGAGAAG CACATTGGAC GCGAGAAGCG AATGGAGGTG 
GCCCAGAAGT GGCAGAAGGA GGCTCAGGAA AGAGCCCTCG AGGAGGAGAA GAAGAAACAG 
GAAGTGGAGC TCAAGATGCG GCGACCATCT CGATTCGAGG TCCTTCCAGC TCCGGATATT 
CTCAGTTTAC GCCAGATTGC CAACGACGAA ATGCTGCCGC CCAAGAAAAG GGCGGAGACA 
ATGCACGGTT CGCTGGCGCC CAGGAAGTCC ATTTTGAAGA AGACCAACTC CTTCAACCTG 
AAGACCTACG CGCAGCCCAT GGGGCACAGT CCCAGCATCA CGCCCTACAC CACGATCACC 
GGCAATTCCG AGTTCCGCAT TCGCTCAGCC TTCGAGGCCA TATTTAAGAA GTCCACCACG 
CTCCAGGACG TACAGCCGGA TCCGGAAACG GGTTCCCTCT CCCCGGCGGC AAGCAACCAC 
GAGGTGGAGG TGCCGCGAAC TCCTAGCACT CCCGGTGTTT CCAAAAAGGT TCAGCTGCCT 
GCACAAAGTA ATTGGGATTT TGTAACAGAT CAAATTATGC TGCAAGTAAA CCCTATTTCA 
ACGGAATCAA ATAAAATGCC TGCCGAGAAA GATTTCACGG ATAAAGCATT ATCAAATAAT 
GGAGATAGTT CAAAGCAATC ACCGAGCATT AAATTCAAAG CAAATAAAGT GGCAGATGTA 
AATAGATCTC CTCAGACGGC CAAGAGGTGC TCGAAAATAA GGTTTGCTAA CGAAGTTGGA 
GTAAATGGTT CGCCCACTCG AACGAAATGT AAAATAAAAC CAGAAAATGT ATTGGGTTAC 
TCTATAGAGG ATGTGGATGA AACAACAAAT CCAGAATCGC TGGCTGAGAG CATTCAAAAG 
CCGAAGTCGG TGCGATTGCC CAGGGAACGT GTTATTGACA TGTCACCGGA GGATCAGAAG 
CAATGGGAGC TCGAGGAAAT GTTGAAGCCC ATCGATTTGC AGAAGGCCAA TGTCCACATC 
GATCCCTCGC CATTTCAGCT GGTGGAACGC ACCTCCATAC TCAAGGTTCA CTCGTTGTTT 
TCCATGGTGG GTATTAACCA CGCCTATGTC ACCAAAATCG GAAGACTTGT TGGTGTCGTG 
GGACTCAAAG AATTGCGAAA AGCCATCGAG GACATTAATA GCAATAGTTT CGTGCCCCCC 
ACACGGGATG AGGATGCGGA TGAGAAGCCG GCGGTGGAGA AACCTCTGCT GTCGACGAAC 
TCTAGTGATA AGGCCGTGGA CATGACCGTC ACCTCCATGG ACTCGGCACT ATCCAATTCC 
GAGAACTGTT CGGACATTGA AATGGAGCAC ATAAAGCACA CGGATAAGGG CACTGTATCG 
CTCACCATGC CGCCACAGGA ATCTAAGCAA AGTCCATCGG CGGACAAAAG TAACACAGAA 
AACGGAAATC ATGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ClC-a cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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