CG6325 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG6325
Entrez Gene ID	41268
Full Name	CG6325 gene product from transcript CG6325-RA
Synonyms	Dmel\CG6325
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 86B4-86B4

mRNA and Protein(s)

mRNA	Protein	Name
NM_141743.3	NP_650000.1	uncharacterized protein

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG6325	NP_650000.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004062	XP_309614.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG6325 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG6325 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa08286	NM_141743.3 Latest version!	Drosophila melanogaster uncharacterized protein (CG6325), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG6325 ( NM_141743.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_650000.1 Drosophila melanogaster uncharacterized protein (CG6325), mRNA.

Price & Availability↑

CloneID	OFa08286
Clone ID Related Accession (Same CDS sequence)	NM_141743.3
Accession Version	NM_141743.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2679bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141743.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGACTG ACTACCAGAA CTACGTGGAG TCGCTGTCGG GTGTGTCCTC CGACGAGGAC 
GACTTCTCCA CGGTGGACAG CTCGGACGAG TCTTCGGAAG AGCCAGTTCC CACGCCATCA 
TCAATCCCAA GGACACGAAA TCTGGTGCAG ATGCTCTACG ACAGAGAGCG CACTGGACAC 
TTCTCAGGCT CCTCCAGGGC TGGCCAAAAG CAACAGCTTC CTTACGTCAG AGTGCCAAAT 
AGGTTAAAGT TATATCTTAA GGATGTTATG GCCAGCAATT TGATGGAGGG ACACATTTTC 
ATGGGTCTAA CTGCCTGCGG CCAGTACATG CTAAGCCATC GGGTGATGTG CAACGACAGC 
TCTTCGCTGA ACCACTACTC CTTTAACATG GGCTACAAAT ACACTCTGTA CTTCTGGCTT 
TTCCAACCGC ACAAGAGGCT GCGCCACTTT TTCTCGCGTC GCCTCTTTGA CGATCATGTG 
GTGGACAACA TAAAGACTGT AACCATGACG CAGTGGAAAA ATGCGCAGCA CCAAATCCTA 
GTTGTCCACG GCGCAGCCAC GGAGGAGGGT GAAGATAGCT ATATCACATA TGTCAAAGTT 
CCTAAACTGG GTTGCTTGGA GTGCAAGAAA CTTAATGACG ACGGTCCATA TTCCTTTTAC 
AATGCGCACT GCCTGACCTG TCACTTAACA GTGCACACAA AATACTCCTC GACTGAAACT 
GATCCGGCTT TCGATCCGAA AATAAATCTG CTTTGCCCGG AGCGCATTCT GATTATCTCC 
AATGGATTTA TGCACATGCT GCGCATCGAT ATGGACACGC CGGCCGTGAC CTCGGGGTAC 
CTTCCCCAGC AGCAGAGCCT TATCTTGCCC AGTCTTCAAG CGCAACCTCA ATCGCAATCT 
TTCTTGCTAC CCAGATCGAT TTTGGCCAGC GAAGAGGATC GCTCCTCAGC TGCATTCACA 
GGTTCCGGAT CGGAGGCAGA GTCTTTAAAT GAACAGAGTG TTGTGGCCCG CATAATTGCC 
GACTTTAGCG ACATTGAAAC CGAGCAGCGC TCTAGTCAAC CCGGTCATAA CCAAAATGAG 
TCGCAGTCCG GGAGCAACGT TTACTCTTCG CCAAAGGCAT ACGATAAGCT AATTTTCACG 
CCACATTGCA GTCCAACTCT GGTTACGGGC CCTAATAGTA TGACTCTGCC GGAGACCACG 
CTGCCGGTCA CAAAACCACT TGTCAACGAA TCTAGTCCGC CACGCAGGCG CAACCATCGG 
ATAGTCACTA AGTTCCGAAA TGGCATTACA ACCATTGATT TGCAGAGCCC TCCCATTGCC 
AATTCGATGA CGGACAAATC GAATGCATAT GAATTCTCAG AGGACAACGA GAAGTACGAA 
AAGATATCCA CTTTCCGAAA GCGACGTTTG GCGGAAAAGA AATATGAGTT CTCGGAAGAC 
AACTCGGAGA ATATCATACC TTTTACCAAG GTGCGATTGG CGGGTAGGGC TTTCAACGCC 
TCCACTGCCA GCACAACGTC TCGCAGCTTC CATCGTTTTT CGCCCACGGC ACACTTCTTT 
CACAACTCGC CCTGTGCTTC GCCCTCCTCT TCGCCACATC CCTCCCAGCC GGCACAGACG 
CCTCCGCATC TGGGATTCCG TTCACCACCG TCCAGTTCCA TGCTAATGCG TTCGCCAAGT 
CGGAATGCAA ACTCGGCTCA GATGTTTAAC CAGAAATCGC CACCATTATC CCAGGCCACA 
CAGCAAATGC TTAGTTACAA GCCAGTGGGT CCACTGGGAG CACTTTCTCC ACTCCAGATG 
TCGTTGGCCA AGCGTTTCGA TATGGGTGGC TCCATGTCCC CAAACGCTGG ATTGAGCTTC 
CTGTCACCGC GACGCGATGA GCCACGTATT GTGGAGATGC CTGTGCAGGG CGGTGTAATG 
CCAGAGAAGC CTGTGTGCAC AAAGAAGCTA AGAAGACGCT ATGTAGAGGA GGACGATGCA 
ACCTCCGTCA TCACCAGCGA AGAGGACGAC TGCATATCAC CGGGCTATCA CACATCGCTG 
CCAATGGAAG TGCACGGCTC ATGTTACTCG GAGATGCAAA TGATTTCACA GGCGTCGTAT 
CATCGGCTGC GCTGTAGTAG CGTCGTAATC ACCCAGCACT CGTTTGATCT GGAGACCTTC 
ACGTACTACG TGATCAGCCT TCTGTGCCAG AAGCATCAGA AGATCTACAA CGTGTTCTAC 
GACTGGGCGT ATGAGGTGAT CAGCGTGTGT CCACTTAGTC AGACTATCGT CTGCATGCTG 
ACGGCCCAAT TCTCGGCGCG GGATCAGCCG CTGGCGATTT GCATACACTG CACTGGCCGA 
ATGGACTGTG TTTTTCACAA CAGGCAGTAC GAAAGCCGCA TCTTGTTCAC CTGGAATATG 
GAGAGCGGTC AATGGCTGGT GCTCGATTAT GGTGAACTTA AAGAGGTGCA CGAGCAGTTC 
AACCCACTAA AGCGGCTTGG CAAGGCACGG CTCACGTTTG CCCAGATGGC CAAGAAGATG 
GGTAAGGAGA TGGCCGTCAG CTTGGGTCTA AACCTGCCCG ACTACACCTC TAATCTGCGC 
GTGCTCAACT CGGATATCCG AAAGTCCAAG ATGTTCATCA GTGATCTCGA CAACATGGTA 
GAGTTCCACT TAAAGCGCAC ACACCACGAG ACACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_650000.1
CDS	269..2947
Translation	METDYQNYVESLSGVSSDEDDFSTVDSSDESSEEPVPTPSSIPRTRNLVQMLYDRERTGHFSGSSRAGQKQQLPYVRVPNRLKLYLKDVMASNLMEGHIFMGLTACGQYMLSHRVMCNDSSSLNHYSFNMGYKYTLYFWLFQPHKRLRHFFSRRLFDDHVVDNIKTVTMTQWKNAQHQILVVHGAATEEGEDSYITYVKVPKLGCLECKKLNDDGPYSFYNAHCLTCHLTVHTKYSSTETDPAFDPKINLLCPERILIISNGFMHMLRIDMDTPAVTSGYLPQQQSLILPSLQAQPQSQSFLLPRSILASEEDRSSAAFTGSGSEAESLNEQSVVARIIADFSDIETEQRSSQPGHNQNESQSGSNVYSSPKAYDKLIFTPHCSPTLVTGPNSMTLPETTLPVTKPLVNESSPPRRRNHRIVTKFRNGITTIDLQSPPIANSMTDKSNAYEFSEDNEKYEKISTFRKRRLAEKKYEFSEDNSENIIPFTKVRLAGRAFNASTASTTSRSFHRFSPTAHFFHNSPCASPSSSPHPSQPAQTPPHLGFRSPPSSSMLMRSPSRNANSAQMFNQKSPPLSQATQQMLSYKPVGPLGALSPLQMSLAKRFDMGGSMSPNAGLSFLSPRRDEPRIVEMPVQGGVMPEKPVCTKKLRRRYVEEDDATSVITSEEDDCISPGYHTSLPMEVHGSCYSEMQMISQASYHRLRCSSVVITQHSFDLETFTYYVISLLCQKHQKIYNVFYDWAYEVISVCPLSQTIVCMLTAQFSARDQPLAICIHCTGRMDCVFHNRQYESRILFTWNMESGQWLVLDYGELKEVHEQFNPLKRLGKARLTFAQMAKKMGKEMAVSLGLNLPDYTSNLRVLNSDIRKSKMFISDLDNMVEFHLKRTHHETL

Target ORF information:

RefSeq Version	NM_141743.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein (CG6325), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141743.3

ORF Insert Sequence:

ATGGAGACTG ACTACCAGAA CTACGTGGAG TCGCTGTCGG GTGTGTCCTC CGACGAGGAC 
GACTTCTCCA CGGTGGACAG CTCGGACGAG TCTTCGGAAG AGCCAGTTCC CACGCCATCA 
TCAATCCCAA GGACACGAAA TCTGGTGCAG ATGCTCTACG ACAGAGAGCG CACTGGACAC 
TTCTCAGGCT CCTCCAGGGC TGGCCAAAAG CAACAGCTTC CTTACGTCAG AGTGCCAAAT 
AGGTTAAAGT TATATCTTAA GGATGTTATG GCCAGCAATT TGATGGAGGG ACACATTTTC 
ATGGGTCTAA CTGCCTGCGG CCAGTACATG CTAAGCCATC GGGTGATGTG CAACGACAGC 
TCTTCGCTGA ACCACTACTC CTTTAACATG GGCTACAAAT ACACTCTGTA CTTCTGGCTT 
TTCCAACCGC ACAAGAGGCT GCGCCACTTT TTCTCGCGTC GCCTCTTTGA CGATCATGTG 
GTGGACAACA TAAAGACTGT AACCATGACG CAGTGGAAAA ATGCGCAGCA CCAAATCCTA 
GTTGTCCACG GCGCAGCCAC GGAGGAGGGT GAAGATAGCT ATATCACATA TGTCAAAGTT 
CCTAAACTGG GTTGCTTGGA GTGCAAGAAA CTTAATGACG ACGGTCCATA TTCCTTTTAC 
AATGCGCACT GCCTGACCTG TCACTTAACA GTGCACACAA AATACTCCTC GACTGAAACT 
GATCCGGCTT TCGATCCGAA AATAAATCTG CTTTGCCCGG AGCGCATTCT GATTATCTCC 
AATGGATTTA TGCACATGCT GCGCATCGAT ATGGACACGC CGGCCGTGAC CTCGGGGTAC 
CTTCCCCAGC AGCAGAGCCT TATCTTGCCC AGTCTTCAAG CGCAACCTCA ATCGCAATCT 
TTCTTGCTAC CCAGATCGAT TTTGGCCAGC GAAGAGGATC GCTCCTCAGC TGCATTCACA 
GGTTCCGGAT CGGAGGCAGA GTCTTTAAAT GAACAGAGTG TTGTGGCCCG CATAATTGCC 
GACTTTAGCG ACATTGAAAC CGAGCAGCGC TCTAGTCAAC CCGGTCATAA CCAAAATGAG 
TCGCAGTCCG GGAGCAACGT TTACTCTTCG CCAAAGGCAT ACGATAAGCT AATTTTCACG 
CCACATTGCA GTCCAACTCT GGTTACGGGC CCTAATAGTA TGACTCTGCC GGAGACCACG 
CTGCCGGTCA CAAAACCACT TGTCAACGAA TCTAGTCCGC CACGCAGGCG CAACCATCGG 
ATAGTCACTA AGTTCCGAAA TGGCATTACA ACCATTGATT TGCAGAGCCC TCCCATTGCC 
AATTCGATGA CGGACAAATC GAATGCATAT GAATTCTCAG AGGACAACGA GAAGTACGAA 
AAGATATCCA CTTTCCGAAA GCGACGTTTG GCGGAAAAGA AATATGAGTT CTCGGAAGAC 
AACTCGGAGA ATATCATACC TTTTACCAAG GTGCGATTGG CGGGTAGGGC TTTCAACGCC 
TCCACTGCCA GCACAACGTC TCGCAGCTTC CATCGTTTTT CGCCCACGGC ACACTTCTTT 
CACAACTCGC CCTGTGCTTC GCCCTCCTCT TCGCCACATC CCTCCCAGCC GGCACAGACG 
CCTCCGCATC TGGGATTCCG TTCACCACCG TCCAGTTCCA TGCTAATGCG TTCGCCAAGT 
CGGAATGCAA ACTCGGCTCA GATGTTTAAC CAGAAATCGC CACCATTATC CCAGGCCACA 
CAGCAAATGC TTAGTTACAA GCCAGTGGGT CCACTGGGAG CACTTTCTCC ACTCCAGATG 
TCGTTGGCCA AGCGTTTCGA TATGGGTGGC TCCATGTCCC CAAACGCTGG ATTGAGCTTC 
CTGTCACCGC GACGCGATGA GCCACGTATT GTGGAGATGC CTGTGCAGGG CGGTGTAATG 
CCAGAGAAGC CTGTGTGCAC AAAGAAGCTA AGAAGACGCT ATGTAGAGGA GGACGATGCA 
ACCTCCGTCA TCACCAGCGA AGAGGACGAC TGCATATCAC CGGGCTATCA CACATCGCTG 
CCAATGGAAG TGCACGGCTC ATGTTACTCG GAGATGCAAA TGATTTCACA GGCGTCGTAT 
CATCGGCTGC GCTGTAGTAG CGTCGTAATC ACCCAGCACT CGTTTGATCT GGAGACCTTC 
ACGTACTACG TGATCAGCCT TCTGTGCCAG AAGCATCAGA AGATCTACAA CGTGTTCTAC 
GACTGGGCGT ATGAGGTGAT CAGCGTGTGT CCACTTAGTC AGACTATCGT CTGCATGCTG 
ACGGCCCAAT TCTCGGCGCG GGATCAGCCG CTGGCGATTT GCATACACTG CACTGGCCGA 
ATGGACTGTG TTTTTCACAA CAGGCAGTAC GAAAGCCGCA TCTTGTTCAC CTGGAATATG 
GAGAGCGGTC AATGGCTGGT GCTCGATTAT GGTGAACTTA AAGAGGTGCA CGAGCAGTTC 
AACCCACTAA AGCGGCTTGG CAAGGCACGG CTCACGTTTG CCCAGATGGC CAAGAAGATG 
GGTAAGGAGA TGGCCGTCAG CTTGGGTCTA AACCTGCCCG ACTACACCTC TAATCTGCGC 
GTGCTCAACT CGGATATCCG AAAGTCCAAG ATGTTCATCA GTGATCTCGA CAACATGGTA 
GAGTTCCACT TAAAGCGCAC ACACCACGAG ACACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG6325 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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