Scm cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Scm
Entrez Gene ID	41168
Full Name	Sex comb on midleg
Synonyms	CG9495,Dmel\CG9495,SCM,Su(z)302,l(3)85Ef,scm
General protein information	Preferred Names Sex comb on midleg Names CG9495 gene product from transcript CG9495-RA CG9495-PA CG9495-PB Scm-PA Scm-PB Sex combs on midleg Sex combs on midlegs sex comb on midleg
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 85E2-85E2\|3-48.5 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001260077.2	NP_001247006.1	Sex comb on midleg, isoform B
NM_169299.2	NP_731385.1	Sex comb on midleg, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Scm coordinates PcG complexes and polymerizes to produce broad domains of PcG silencing
Title: Sex comb on midleg (Scm) is a functional link between PcG-repressive complexes in Drosophila.

loss-of-function mutations in Scm result in non-autonomous tissue overgrowth in Drosophila, and determine that Scm is essential for ommatidium development and important for cell survival in Drosophila
Title: A genetic screen in Drosophila implicates Sex comb on midleg (Scm) in tissue overgrowth and mechanisms of Scm degradation by Wds.

SUMO negatively regulates Scm function by impeding its recruitment to the Ubx major PRE.
Title: Small ubiquitin-like modifier (SUMO) conjugation impedes transcriptional silencing by the polycomb group repressor Sex Comb on Midleg.

Data show that Sex Comb on Midleg (SCM) associates with a Polycomb response element upstream of the Ubx gene which also binds PRC1, PRC2, and the DNA-binding PcG protein Pleiohomeotic.
Title: Comparative analysis of chromatin binding by Sex Comb on Midleg (SCM) and other polycomb group repressors at a Drosophila Hox gene.

Testing the role of this structure in male mating success in Drosophila melanogaster, the sex comb was removed by expressing the female-specific isoform of the sex determination gene transformer in the tarsal segments of male legs.
Title: Sex combs are important for male mating success in Drosophila melanogaster.

The following Scm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Scm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04359	NM_001260077.2 Latest version!	Drosophila melanogaster Sex comb on midleg (Scm), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa04359	NM_169299.2 Latest version!	Drosophila melanogaster Sex comb on midleg (Scm), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Scm ( NM_001260077.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247006.1 Drosophila melanogaster Sex comb on midleg (Scm), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa04359
Clone ID Related Accession (Same CDS sequence)	NM_001260077.2 , NM_169299.2
Accession Version	NM_001260077.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Sex comb on midleg, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001260077.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGGCG GACGTGATAG CAGTACCAGT AGCGGGAGCA ATTCTGCTGC GCCAGGGGCC 
AGTACGAATG CCACCTCCTC CGCATCCGCA TCCGCCTCCT CCACATCCAC ATCCGCATCC 
CCTGGAAGTA CAACATCGCC CGCGTCCACG CAACGGCAAC GAGGTAGACC GGCCAAACGA 
GCCACATGCA CCTGGTGCGG CGAGGGCAAG CTACCGCTGC AGTACGTCCT GCCCACGCAG 
ACCGGCAAGA AGGAGTTCTG CTCGGAAACA TGCATTGCCG AGTTCCGCAA GGCGTACAGC 
AAGGGGGCGT GCACCCAGTG CGACAACGTC ATCCGCGATG GGGCGCCCAA CAAGGAGTTC 
TGCTCGATCA TGTGCATGAA CAAGCACCAG AAGAAGAACT GCTCGACCAG GCACAGCGGC 
GGATCGGCAT CGGGCAAGGG TCTGGCGGAA AGCGAGCGCA AGTTGCTGGC CAGTGGAGCG 
CCCGCACCAA CGGGGCCCTT TCAATACGAG AGCTTCCACG TCTTCGACTG GGATGCCTAT 
CTAGAGGAAA CGGGTAGCGA AGCTGCTCCT GCAAAATGTT TCAAACAGGC TCAGAATCCT 
CCCAACAATG ATTTTAAAAT CGGAATGAAG CTGGAGGCTT TGGATCCGCG CAACGTCACT 
TCCACCTGCA TTGCCACCGT GGTCGGAGTG CTTGGCTCCA GACTGCGGCT ACGCCTGGAT 
GGAAGTGACT CCCAGAATGA CTTTTGGCGG CTGGTCGATT CTACGGAGAT CCATGCTATT 
GGACACTGTG AAAAGAACGG TGGCATGCTG CAGCCGCCTC TGGGATTCCG CATGAACGCA 
TCCAGCTGGC CCGGCTATCT GTGCAAGATC CTCAACAATG CCATGGTGGC GCCAGAAGAG 
ATCTTCCAGC CAGAGCCGCC AGAGCCAGAA GAAAACCTGT TCAAGGTGGG CCAGAAACTC 
GAGGCTGTCG ACAAGAAGAA CCCGCAGCTC ATCTGCTGTG CAACAGTAGA TGCAATCAAG 
GATGATCAGA TCCATGTAAC ATTCGATGGT TGGCGAGGGG CATTCGACTA CTGGTGCAAT 
TACCGATCGC GTGACATTTT TCCCGCCGGC TGGTGCGCTA GAAGTTGTCA TCCCATGCAG 
CCACCGGGCC ATAAATCGCG CATGGACTCC AGCTCTAGCA AACAGCGATG CCCTCGACCC 
CGCTACACTG TTGTAGCTGA GTCCGAGGCT ATGGTTCCCG CTTCCCCTGC CACAGCCCAC 
TTCCATCCGA ACTGCAAGGG CGGTCCGTTC ATCAACAATT CCAAACTGCC CTGCATGGTG 
ACGGGACCCA CCTACCAAAC GCTGGCCAAG CTTTGCCTGC AGGAGGTTTT AGCTGCCAGC 
ACGGATACGC AACAGCTCTC AAAGTTGCTG TTTGCCCTGG AAGGAGATGT TCATATCGTG 
ACGGCAGCGG GGAAAAATTT CACAGTAAAA ATACCATCGC CAATGCGAAT GAAGGATGAC 
GAAAGCCTCG CCCAATTCAT CGAAACGCTG TGCACTACAT GCCGGGCATG TGCGAATCTC 
ATTTCCTTGG TCCACGAGAC GGAGGAGTGC AAGAAGTGCG CCAATAGTCG GAAGCGTCAA 
CTGACTCAGT CAGCCACACC GCCTTCCTCG CCAGTGCTCG CCGACAAGCG AAACCGTCAA 
TCCAACTCGG CAACGACATC ACCCTCTGAG AAGATTATCA AGCAGGAATT GGCTGTCAAA 
TCGCCAGTGG AGTCCAAGTC GAAAACTTCG ACGAACAATG GCAAGGAGCC TGCAAGCCAG 
CAAAACAGCA ATCACAGTCT CAACAATAAC AACAACAGTG CCAGCAAGTC CAGCAACAAG 
GTGGTCATCA AATCGGAGCC CAACGGAGCG AACGCGCAGA CTTCCTCCAC AACGCAGGCT 
CTGCGCAAAG TACGCTTCCA ACACCACGCA AACACGAATA CGAATAGCTC TGCGACAAAC 
GGAAACCAGG ATACGAGCCA GACCACGCAC GTATCTACCA GCCATTGCAG CAGCTCGTCA 
ACGTCGTCCA GCACGAGCCT AGCCGGAGGA TCGGCGAACA CCAGCACAAT TGGCAAATAC 
CTGGCACCTC TTGTGGCAGA AGTTCATCCA GAGCAGGCCA ATGTAAAGCC CTCGAATAGC 
TACTACAAGA GTCCGACGAC GCTCTCGTCA AGCGCCTCGT TGCCGACATC CGTGTCCACG 
CCGTTCACGG GCTGCCAGTC AGCCTCGTCC ACTGCACTGG CTGCAGGTGG AGTTACAGCG 
GCTAAGGCGG CCACCGCGCC GGCTGGAGCA GCGGCAACTG CGGGAGCTTC TCCGAGCTAC 
ACCGCGATTA CCTCACCCGT GAGCACGCCC ACTTCTGCTT TGGCAAACTC ACATCTGCGG 
TCGCAGCCGA TCGACTGGAC CATCGAAGAG GTGATCCAGT ACATCGAGAG CAACGACAAC 
TCCCTTGCAG TTCATGGTGA TCTCTTCCGA AAGCACGAAA TCGATGGTAA AGCTTTATTA 
TTGCTAAATT CAGAGATGAT GATGAAGTAC ATGGGCCTGA AGCTAGGACC AGCCTTAAAA 
ATCTGCAATT TAGTCAATAA GGTCAATGGT CGTCGAAATA ATCTGGCACT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247006.1
CDS	457..3090
Translation	MSGGRDSSTSSGSNSAAPGASTNATSSASASASSTSTSASPGSTTSPASTQRQRGRPAKRATCTWCGEGKLPLQYVLPTQTGKKEFCSETCIAEFRKAYSKGACTQCDNVIRDGAPNKEFCSIMCMNKHQKKNCSTRHSGGSASGKGLAESERKLLASGAPAPTGPFQYESFHVFDWDAYLEETGSEAAPAKCFKQAQNPPNNDFKIGMKLEALDPRNVTSTCIATVVGVLGSRLRLRLDGSDSQNDFWRLVDSTEIHAIGHCEKNGGMLQPPLGFRMNASSWPGYLCKILNNAMVAPEEIFQPEPPEPEENLFKVGQKLEAVDKKNPQLICCATVDAIKDDQIHVTFDGWRGAFDYWCNYRSRDIFPAGWCARSCHPMQPPGHKSRMDSSSSKQRCPRPRYTVVAESEAMVPASPATAHFHPNCKGGPFINNSKLPCMVTGPTYQTLAKLCLQEVLAASTDTQQLSKLLFALEGDVHIVTAAGKNFTVKIPSPMRMKDDESLAQFIETLCTTCRACANLISLVHETEECKKCANSRKRQLTQSATPPSSPVLADKRNRQSNSATTSPSEKIIKQELAVKSPVESKSKTSTNNGKEPASQQNSNHSLNNNNNSASKSSNKVVIKSEPNGANAQTSSTTQALRKVRFQHHANTNTNSSATNGNQDTSQTTHVSTSHCSSSSTSSSTSLAGGSANTSTIGKYLAPLVAEVHPEQANVKPSNSYYKSPTTLSSSASLPTSVSTPFTGCQSASSTALAAGGVTAAKAATAPAGAAATAGASPSYTAITSPVSTPTSALANSHLRSQPIDWTIEEVIQYIESNDNSLAVHGDLFRKHEIDGKALLLLNSEMMMKYMGLKLGPALKICNLVNKVNGRRNNLAL

Target ORF information:

RefSeq Version	NM_001260077.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Sex comb on midleg (Scm), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260077.2

ORF Insert Sequence:

ATGTCGGGCG GACGTGATAG CAGTACCAGT AGCGGGAGCA ATTCTGCTGC GCCAGGGGCC 
AGTACGAATG CCACCTCCTC CGCATCCGCA TCCGCCTCCT CCACATCCAC ATCCGCATCC 
CCTGGAAGTA CAACATCGCC CGCGTCCACG CAACGGCAAC GAGGTAGACC GGCCAAACGA 
GCCACATGCA CCTGGTGCGG CGAGGGCAAG CTACCGCTGC AGTACGTCCT GCCCACGCAG 
ACCGGCAAGA AGGAGTTCTG CTCGGAAACA TGCATTGCCG AGTTCCGCAA GGCGTACAGC 
AAGGGGGCGT GCACCCAGTG CGACAACGTC ATCCGCGATG GGGCGCCCAA CAAGGAGTTC 
TGCTCGATCA TGTGCATGAA CAAGCACCAG AAGAAGAACT GCTCGACCAG GCACAGCGGC 
GGATCGGCAT CGGGCAAGGG TCTGGCGGAA AGCGAGCGCA AGTTGCTGGC CAGTGGAGCG 
CCCGCACCAA CGGGGCCCTT TCAATACGAG AGCTTCCACG TCTTCGACTG GGATGCCTAT 
CTAGAGGAAA CGGGTAGCGA AGCTGCTCCT GCAAAATGTT TCAAACAGGC TCAGAATCCT 
CCCAACAATG ATTTTAAAAT CGGAATGAAG CTGGAGGCTT TGGATCCGCG CAACGTCACT 
TCCACCTGCA TTGCCACCGT GGTCGGAGTG CTTGGCTCCA GACTGCGGCT ACGCCTGGAT 
GGAAGTGACT CCCAGAATGA CTTTTGGCGG CTGGTCGATT CTACGGAGAT CCATGCTATT 
GGACACTGTG AAAAGAACGG TGGCATGCTG CAGCCGCCTC TGGGATTCCG CATGAACGCA 
TCCAGCTGGC CCGGCTATCT GTGCAAGATC CTCAACAATG CCATGGTGGC GCCAGAAGAG 
ATCTTCCAGC CAGAGCCGCC AGAGCCAGAA GAAAACCTGT TCAAGGTGGG CCAGAAACTC 
GAGGCTGTCG ACAAGAAGAA CCCGCAGCTC ATCTGCTGTG CAACAGTAGA TGCAATCAAG 
GATGATCAGA TCCATGTAAC ATTCGATGGT TGGCGAGGGG CATTCGACTA CTGGTGCAAT 
TACCGATCGC GTGACATTTT TCCCGCCGGC TGGTGCGCTA GAAGTTGTCA TCCCATGCAG 
CCACCGGGCC ATAAATCGCG CATGGACTCC AGCTCTAGCA AACAGCGATG CCCTCGACCC 
CGCTACACTG TTGTAGCTGA GTCCGAGGCT ATGGTTCCCG CTTCCCCTGC CACAGCCCAC 
TTCCATCCGA ACTGCAAGGG CGGTCCGTTC ATCAACAATT CCAAACTGCC CTGCATGGTG 
ACGGGACCCA CCTACCAAAC GCTGGCCAAG CTTTGCCTGC AGGAGGTTTT AGCTGCCAGC 
ACGGATACGC AACAGCTCTC AAAGTTGCTG TTTGCCCTGG AAGGAGATGT TCATATCGTG 
ACGGCAGCGG GGAAAAATTT CACAGTAAAA ATACCATCGC CAATGCGAAT GAAGGATGAC 
GAAAGCCTCG CCCAATTCAT CGAAACGCTG TGCACTACAT GCCGGGCATG TGCGAATCTC 
ATTTCCTTGG TCCACGAGAC GGAGGAGTGC AAGAAGTGCG CCAATAGTCG GAAGCGTCAA 
CTGACTCAGT CAGCCACACC GCCTTCCTCG CCAGTGCTCG CCGACAAGCG AAACCGTCAA 
TCCAACTCGG CAACGACATC ACCCTCTGAG AAGATTATCA AGCAGGAATT GGCTGTCAAA 
TCGCCAGTGG AGTCCAAGTC GAAAACTTCG ACGAACAATG GCAAGGAGCC TGCAAGCCAG 
CAAAACAGCA ATCACAGTCT CAACAATAAC AACAACAGTG CCAGCAAGTC CAGCAACAAG 
GTGGTCATCA AATCGGAGCC CAACGGAGCG AACGCGCAGA CTTCCTCCAC AACGCAGGCT 
CTGCGCAAAG TACGCTTCCA ACACCACGCA AACACGAATA CGAATAGCTC TGCGACAAAC 
GGAAACCAGG ATACGAGCCA GACCACGCAC GTATCTACCA GCCATTGCAG CAGCTCGTCA 
ACGTCGTCCA GCACGAGCCT AGCCGGAGGA TCGGCGAACA CCAGCACAAT TGGCAAATAC 
CTGGCACCTC TTGTGGCAGA AGTTCATCCA GAGCAGGCCA ATGTAAAGCC CTCGAATAGC 
TACTACAAGA GTCCGACGAC GCTCTCGTCA AGCGCCTCGT TGCCGACATC CGTGTCCACG 
CCGTTCACGG GCTGCCAGTC AGCCTCGTCC ACTGCACTGG CTGCAGGTGG AGTTACAGCG 
GCTAAGGCGG CCACCGCGCC GGCTGGAGCA GCGGCAACTG CGGGAGCTTC TCCGAGCTAC 
ACCGCGATTA CCTCACCCGT GAGCACGCCC ACTTCTGCTT TGGCAAACTC ACATCTGCGG 
TCGCAGCCGA TCGACTGGAC CATCGAAGAG GTGATCCAGT ACATCGAGAG CAACGACAAC 
TCCCTTGCAG TTCATGGTGA TCTCTTCCGA AAGCACGAAA TCGATGGTAA AGCTTTATTA 
TTGCTAAATT CAGAGATGAT GATGAAGTAC ATGGGCCTGA AGCTAGGACC AGCCTTAAAA 
ATCTGCAATT TAGTCAATAA GGTCAATGGT CGTCGAAATA ATCTGGCACT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scm ( NM_169299.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731385.1 Drosophila melanogaster Sex comb on midleg (Scm), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa04359
Clone ID Related Accession (Same CDS sequence)	NM_001260077.2 , NM_169299.2
Accession Version	NM_169299.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Sex comb on midleg, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_169299.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGGGCG GACGTGATAG CAGTACCAGT AGCGGGAGCA ATTCTGCTGC GCCAGGGGCC 
AGTACGAATG CCACCTCCTC CGCATCCGCA TCCGCCTCCT CCACATCCAC ATCCGCATCC 
CCTGGAAGTA CAACATCGCC CGCGTCCACG CAACGGCAAC GAGGTAGACC GGCCAAACGA 
GCCACATGCA CCTGGTGCGG CGAGGGCAAG CTACCGCTGC AGTACGTCCT GCCCACGCAG 
ACCGGCAAGA AGGAGTTCTG CTCGGAAACA TGCATTGCCG AGTTCCGCAA GGCGTACAGC 
AAGGGGGCGT GCACCCAGTG CGACAACGTC ATCCGCGATG GGGCGCCCAA CAAGGAGTTC 
TGCTCGATCA TGTGCATGAA CAAGCACCAG AAGAAGAACT GCTCGACCAG GCACAGCGGC 
GGATCGGCAT CGGGCAAGGG TCTGGCGGAA AGCGAGCGCA AGTTGCTGGC CAGTGGAGCG 
CCCGCACCAA CGGGGCCCTT TCAATACGAG AGCTTCCACG TCTTCGACTG GGATGCCTAT 
CTAGAGGAAA CGGGTAGCGA AGCTGCTCCT GCAAAATGTT TCAAACAGGC TCAGAATCCT 
CCCAACAATG ATTTTAAAAT CGGAATGAAG CTGGAGGCTT TGGATCCGCG CAACGTCACT 
TCCACCTGCA TTGCCACCGT GGTCGGAGTG CTTGGCTCCA GACTGCGGCT ACGCCTGGAT 
GGAAGTGACT CCCAGAATGA CTTTTGGCGG CTGGTCGATT CTACGGAGAT CCATGCTATT 
GGACACTGTG AAAAGAACGG TGGCATGCTG CAGCCGCCTC TGGGATTCCG CATGAACGCA 
TCCAGCTGGC CCGGCTATCT GTGCAAGATC CTCAACAATG CCATGGTGGC GCCAGAAGAG 
ATCTTCCAGC CAGAGCCGCC AGAGCCAGAA GAAAACCTGT TCAAGGTGGG CCAGAAACTC 
GAGGCTGTCG ACAAGAAGAA CCCGCAGCTC ATCTGCTGTG CAACAGTAGA TGCAATCAAG 
GATGATCAGA TCCATGTAAC ATTCGATGGT TGGCGAGGGG CATTCGACTA CTGGTGCAAT 
TACCGATCGC GTGACATTTT TCCCGCCGGC TGGTGCGCTA GAAGTTGTCA TCCCATGCAG 
CCACCGGGCC ATAAATCGCG CATGGACTCC AGCTCTAGCA AACAGCGATG CCCTCGACCC 
CGCTACACTG TTGTAGCTGA GTCCGAGGCT ATGGTTCCCG CTTCCCCTGC CACAGCCCAC 
TTCCATCCGA ACTGCAAGGG CGGTCCGTTC ATCAACAATT CCAAACTGCC CTGCATGGTG 
ACGGGACCCA CCTACCAAAC GCTGGCCAAG CTTTGCCTGC AGGAGGTTTT AGCTGCCAGC 
ACGGATACGC AACAGCTCTC AAAGTTGCTG TTTGCCCTGG AAGGAGATGT TCATATCGTG 
ACGGCAGCGG GGAAAAATTT CACAGTAAAA ATACCATCGC CAATGCGAAT GAAGGATGAC 
GAAAGCCTCG CCCAATTCAT CGAAACGCTG TGCACTACAT GCCGGGCATG TGCGAATCTC 
ATTTCCTTGG TCCACGAGAC GGAGGAGTGC AAGAAGTGCG CCAATAGTCG GAAGCGTCAA 
CTGACTCAGT CAGCCACACC GCCTTCCTCG CCAGTGCTCG CCGACAAGCG AAACCGTCAA 
TCCAACTCGG CAACGACATC ACCCTCTGAG AAGATTATCA AGCAGGAATT GGCTGTCAAA 
TCGCCAGTGG AGTCCAAGTC GAAAACTTCG ACGAACAATG GCAAGGAGCC TGCAAGCCAG 
CAAAACAGCA ATCACAGTCT CAACAATAAC AACAACAGTG CCAGCAAGTC CAGCAACAAG 
GTGGTCATCA AATCGGAGCC CAACGGAGCG AACGCGCAGA CTTCCTCCAC AACGCAGGCT 
CTGCGCAAAG TACGCTTCCA ACACCACGCA AACACGAATA CGAATAGCTC TGCGACAAAC 
GGAAACCAGG ATACGAGCCA GACCACGCAC GTATCTACCA GCCATTGCAG CAGCTCGTCA 
ACGTCGTCCA GCACGAGCCT AGCCGGAGGA TCGGCGAACA CCAGCACAAT TGGCAAATAC 
CTGGCACCTC TTGTGGCAGA AGTTCATCCA GAGCAGGCCA ATGTAAAGCC CTCGAATAGC 
TACTACAAGA GTCCGACGAC GCTCTCGTCA AGCGCCTCGT TGCCGACATC CGTGTCCACG 
CCGTTCACGG GCTGCCAGTC AGCCTCGTCC ACTGCACTGG CTGCAGGTGG AGTTACAGCG 
GCTAAGGCGG CCACCGCGCC GGCTGGAGCA GCGGCAACTG CGGGAGCTTC TCCGAGCTAC 
ACCGCGATTA CCTCACCCGT GAGCACGCCC ACTTCTGCTT TGGCAAACTC ACATCTGCGG 
TCGCAGCCGA TCGACTGGAC CATCGAAGAG GTGATCCAGT ACATCGAGAG CAACGACAAC 
TCCCTTGCAG TTCATGGTGA TCTCTTCCGA AAGCACGAAA TCGATGGTAA AGCTTTATTA 
TTGCTAAATT CAGAGATGAT GATGAAGTAC ATGGGCCTGA AGCTAGGACC AGCCTTAAAA 
ATCTGCAATT TAGTCAATAA GGTCAATGGT CGTCGAAATA ATCTGGCACT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731385.1
CDS	457..3090
Translation	MSGGRDSSTSSGSNSAAPGASTNATSSASASASSTSTSASPGSTTSPASTQRQRGRPAKRATCTWCGEGKLPLQYVLPTQTGKKEFCSETCIAEFRKAYSKGACTQCDNVIRDGAPNKEFCSIMCMNKHQKKNCSTRHSGGSASGKGLAESERKLLASGAPAPTGPFQYESFHVFDWDAYLEETGSEAAPAKCFKQAQNPPNNDFKIGMKLEALDPRNVTSTCIATVVGVLGSRLRLRLDGSDSQNDFWRLVDSTEIHAIGHCEKNGGMLQPPLGFRMNASSWPGYLCKILNNAMVAPEEIFQPEPPEPEENLFKVGQKLEAVDKKNPQLICCATVDAIKDDQIHVTFDGWRGAFDYWCNYRSRDIFPAGWCARSCHPMQPPGHKSRMDSSSSKQRCPRPRYTVVAESEAMVPASPATAHFHPNCKGGPFINNSKLPCMVTGPTYQTLAKLCLQEVLAASTDTQQLSKLLFALEGDVHIVTAAGKNFTVKIPSPMRMKDDESLAQFIETLCTTCRACANLISLVHETEECKKCANSRKRQLTQSATPPSSPVLADKRNRQSNSATTSPSEKIIKQELAVKSPVESKSKTSTNNGKEPASQQNSNHSLNNNNNSASKSSNKVVIKSEPNGANAQTSSTTQALRKVRFQHHANTNTNSSATNGNQDTSQTTHVSTSHCSSSSTSSSTSLAGGSANTSTIGKYLAPLVAEVHPEQANVKPSNSYYKSPTTLSSSASLPTSVSTPFTGCQSASSTALAAGGVTAAKAATAPAGAAATAGASPSYTAITSPVSTPTSALANSHLRSQPIDWTIEEVIQYIESNDNSLAVHGDLFRKHEIDGKALLLLNSEMMMKYMGLKLGPALKICNLVNKVNGRRNNLAL

Target ORF information:

RefSeq Version	NM_169299.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Sex comb on midleg (Scm), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169299.2

ORF Insert Sequence:

ATGTCGGGCG GACGTGATAG CAGTACCAGT AGCGGGAGCA ATTCTGCTGC GCCAGGGGCC 
AGTACGAATG CCACCTCCTC CGCATCCGCA TCCGCCTCCT CCACATCCAC ATCCGCATCC 
CCTGGAAGTA CAACATCGCC CGCGTCCACG CAACGGCAAC GAGGTAGACC GGCCAAACGA 
GCCACATGCA CCTGGTGCGG CGAGGGCAAG CTACCGCTGC AGTACGTCCT GCCCACGCAG 
ACCGGCAAGA AGGAGTTCTG CTCGGAAACA TGCATTGCCG AGTTCCGCAA GGCGTACAGC 
AAGGGGGCGT GCACCCAGTG CGACAACGTC ATCCGCGATG GGGCGCCCAA CAAGGAGTTC 
TGCTCGATCA TGTGCATGAA CAAGCACCAG AAGAAGAACT GCTCGACCAG GCACAGCGGC 
GGATCGGCAT CGGGCAAGGG TCTGGCGGAA AGCGAGCGCA AGTTGCTGGC CAGTGGAGCG 
CCCGCACCAA CGGGGCCCTT TCAATACGAG AGCTTCCACG TCTTCGACTG GGATGCCTAT 
CTAGAGGAAA CGGGTAGCGA AGCTGCTCCT GCAAAATGTT TCAAACAGGC TCAGAATCCT 
CCCAACAATG ATTTTAAAAT CGGAATGAAG CTGGAGGCTT TGGATCCGCG CAACGTCACT 
TCCACCTGCA TTGCCACCGT GGTCGGAGTG CTTGGCTCCA GACTGCGGCT ACGCCTGGAT 
GGAAGTGACT CCCAGAATGA CTTTTGGCGG CTGGTCGATT CTACGGAGAT CCATGCTATT 
GGACACTGTG AAAAGAACGG TGGCATGCTG CAGCCGCCTC TGGGATTCCG CATGAACGCA 
TCCAGCTGGC CCGGCTATCT GTGCAAGATC CTCAACAATG CCATGGTGGC GCCAGAAGAG 
ATCTTCCAGC CAGAGCCGCC AGAGCCAGAA GAAAACCTGT TCAAGGTGGG CCAGAAACTC 
GAGGCTGTCG ACAAGAAGAA CCCGCAGCTC ATCTGCTGTG CAACAGTAGA TGCAATCAAG 
GATGATCAGA TCCATGTAAC ATTCGATGGT TGGCGAGGGG CATTCGACTA CTGGTGCAAT 
TACCGATCGC GTGACATTTT TCCCGCCGGC TGGTGCGCTA GAAGTTGTCA TCCCATGCAG 
CCACCGGGCC ATAAATCGCG CATGGACTCC AGCTCTAGCA AACAGCGATG CCCTCGACCC 
CGCTACACTG TTGTAGCTGA GTCCGAGGCT ATGGTTCCCG CTTCCCCTGC CACAGCCCAC 
TTCCATCCGA ACTGCAAGGG CGGTCCGTTC ATCAACAATT CCAAACTGCC CTGCATGGTG 
ACGGGACCCA CCTACCAAAC GCTGGCCAAG CTTTGCCTGC AGGAGGTTTT AGCTGCCAGC 
ACGGATACGC AACAGCTCTC AAAGTTGCTG TTTGCCCTGG AAGGAGATGT TCATATCGTG 
ACGGCAGCGG GGAAAAATTT CACAGTAAAA ATACCATCGC CAATGCGAAT GAAGGATGAC 
GAAAGCCTCG CCCAATTCAT CGAAACGCTG TGCACTACAT GCCGGGCATG TGCGAATCTC 
ATTTCCTTGG TCCACGAGAC GGAGGAGTGC AAGAAGTGCG CCAATAGTCG GAAGCGTCAA 
CTGACTCAGT CAGCCACACC GCCTTCCTCG CCAGTGCTCG CCGACAAGCG AAACCGTCAA 
TCCAACTCGG CAACGACATC ACCCTCTGAG AAGATTATCA AGCAGGAATT GGCTGTCAAA 
TCGCCAGTGG AGTCCAAGTC GAAAACTTCG ACGAACAATG GCAAGGAGCC TGCAAGCCAG 
CAAAACAGCA ATCACAGTCT CAACAATAAC AACAACAGTG CCAGCAAGTC CAGCAACAAG 
GTGGTCATCA AATCGGAGCC CAACGGAGCG AACGCGCAGA CTTCCTCCAC AACGCAGGCT 
CTGCGCAAAG TACGCTTCCA ACACCACGCA AACACGAATA CGAATAGCTC TGCGACAAAC 
GGAAACCAGG ATACGAGCCA GACCACGCAC GTATCTACCA GCCATTGCAG CAGCTCGTCA 
ACGTCGTCCA GCACGAGCCT AGCCGGAGGA TCGGCGAACA CCAGCACAAT TGGCAAATAC 
CTGGCACCTC TTGTGGCAGA AGTTCATCCA GAGCAGGCCA ATGTAAAGCC CTCGAATAGC 
TACTACAAGA GTCCGACGAC GCTCTCGTCA AGCGCCTCGT TGCCGACATC CGTGTCCACG 
CCGTTCACGG GCTGCCAGTC AGCCTCGTCC ACTGCACTGG CTGCAGGTGG AGTTACAGCG 
GCTAAGGCGG CCACCGCGCC GGCTGGAGCA GCGGCAACTG CGGGAGCTTC TCCGAGCTAC 
ACCGCGATTA CCTCACCCGT GAGCACGCCC ACTTCTGCTT TGGCAAACTC ACATCTGCGG 
TCGCAGCCGA TCGACTGGAC CATCGAAGAG GTGATCCAGT ACATCGAGAG CAACGACAAC 
TCCCTTGCAG TTCATGGTGA TCTCTTCCGA AAGCACGAAA TCGATGGTAA AGCTTTATTA 
TTGCTAAATT CAGAGATGAT GATGAAGTAC ATGGGCCTGA AGCTAGGACC AGCCTTAAAA 
ATCTGCAATT TAGTCAATAA GGTCAATGGT CGTCGAAATA ATCTGGCACT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Scm cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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