by cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	by
Entrez Gene ID	41144
Full Name	blistery
Synonyms	3R-B,BcDNA:RE65113,CG9379,CT26639,Dmel\CG9379
General protein information	Preferred Names blistery Names CG9379 gene product from transcript CG9379-RA 3R-B CG9379-PA CG9379-PC CG9379-PD by-PA by-PC by-PD tensin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 85D19-85D22\|3-48.7 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001170099.3	NP_001163570.1	blistery, isoform C
NM_141644.4	NP_649901.1	blistery, isoform A
NM_001170098.2	NP_001163569.1	blistery, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study suggest tensin is recruited to sites of integrin adhesion via its PTB and N-terminal domains, localizing the SH2 domain so that it can interact with phosphotyrosine-containing proteins, which stabilize the integrin link to the cytoskeleton.
Title: Tensin stabilizes integrin adhesive contacts in Drosophila.

The following by gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the by cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04333	NM_001170099.3 Latest version!	Drosophila melanogaster blistery (by), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa04333	NM_001170098.2 Latest version!	Drosophila melanogaster blistery (by), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa04333	NM_141644.4 Latest version!	Drosophila melanogaster blistery (by), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

by ( NM_001170099.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163570.1 Drosophila melanogaster blistery (by), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa04333
Clone ID Related Accession (Same CDS sequence)	NM_001170099.3 , NM_141644.4 , NM_001170098.2
Accession Version	NM_001170099.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	blistery, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170099.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGGACGC CGTACGAAGA AAGCGCTGCT GCCGCCGACC ACAAATATCA ATACCAATAC 
ACCGCGGCCA ATAATAACAA TAATTACTAT AAAAACTATA ATGTGAATGG CAATGGGCAG 
GCGAATGGAA ATGGGAATCT AAGTGGAATT GGTAATGGAA ATGGAATTGG AAACGGCAAT 
GGATATGGAA TACCTTATCA CGCGCGCGAG AACTCGCTGC CATTTAGCTA TGGCATAGCC 
AAAACTTCGA CACCGTTGCG AAAAACTCCG ACACCGACGC GAGTGGATAG TTTTCTAGAC 
TTTGATTTCG GGCCAACGAA TACGAATGTG AATTCGAATA GTTCTAGCAA TAATGGCGGT 
ACTGGCGATA TGATAAAGCC ACCAACACGT CTAACGAGCC CTTTGCTGGT GCGCAAAACG 
CTGGGCAATG CCGTTAGTCC CACCAGTTCG ACCAATACCA ACACCATAGC CAATACATAC 
CACTACACGT CGAGCAGCGC AACGCCGACG CCGGTGAGAG ATAATTTCGA GCAGCTGCTG 
CGGGAGCGAC GCGAAAAGGT CTACAATGAA TATAGGAATC TGGATAGGGA GTCGTCCGGG 
GAGCGGGCCA GAGTGGCACC CGCTCCGCCG CAGAGGCAAT ACTACCCGAG CGATGGCTAT 
GGAATTGGGC TGAAGAACGA TAGCAATGGA ACCCTATACC GCAAGAGCCC CTCGTCTAAT 
GGGAAATCAA CGAAATACAA CTACGATTTT GAGTTCAATC TGAATCTGGA TGATGTTAGG 
CCCGAACCTA CACCGTATAC CACGAGGAAT ATTCACTTCG AGGATCTGCG GCTGGATCAG 
GATGTGCCAG ATGGCGTGGT CAATCGGCGG ACCATGGAAC GCAATTATCA CACAATTAAC 
AGCTTGGAAA CGACCCACAA ACGCCAGCAG TTGGAGCCGT TGGAGCAACT GGGCGCCGTT 
TATGGCCAGA ACCACATGGA GGAGACACTC ACGCGCACTC GTCGCGATCT CTCCGCATCC 
ACATCGACAT CCAAGTCCAC CTCGCCATTG CCGGCAATAG GAGTACCAGC TATAGCACCA 
GCTTCGCCCA TTTATGCCAC CAGTACGAAG AGAGTGAGCA ACCCAAATAC CAATGGCCAA 
CAGCAGATGA CGCCTAGTCC CACCAGTAGG CCAGAAACGC CCGCCTTTCC GGTCACGCCG 
CGCACGCCTT TTGGGGCATC ATCATCATCA TCTGCGTCTC TGGCGCCGAC GACCCAGTTG 
CCCCCAGAGT CGATCTACCA AACAGGGCCA CGTCGTGGCG TACCGCCTGC ACTGCAGCCA 
CTCGAGGTGT CCGCCGATTC CGTACAATTT GCCCGTAGTT CGTCCCAGTT CTGGTACAAA 
CCAAATCTCA CTCGCGAAGA TGCCATCGCT CTGCTGGCCT CTGCCCAGCC GGGCACGTTC 
CTGGTCAGGG ACTCCACCAC GTACAAGGAT TCCTATGGCC TGGTGGTGCG GGTGTCCCAA 
CCGCCTCCCG GTTCACAGGA GCTGGTGCGT CACTTCCTCA TCGAGCCCAC GAAGGGGGGA 
GTGCATCTGA AGGGCTGCGA TGACGAGCCG GTGTTCACTT CACTGTCCGC CCTCGTCTTC 
GAGCATTCCA TAAGCCAGCT GGCGTTGCCA TGTCTCCTCC GTCTGCCCGA TCGGGATATA 
GTACCACCGG TGCGGGCCAC AACCCCTGCC CAGCAGCATC TATTGGCCCA CGGAGCAGCC 
ACCAATGTGC TGTGGCTCTA CTCCTGCGAC ACGGAATCGC TGACAGGAAA CGAGGCCATC 
CGCAAGGCCA TCCGCCAGAT GTACGGTCAG CAACCCTTGC CGCAGCCCAC GGAGGTGCAC 
TTCAAGGTCT CCTCGCAGGG CATCACGCTC ACGGACAATA CGCGCAAGAA GTTCTTCCGG 
AAGCACTACA AGGCGGATGT TATCTCGCAC TGTGCCATCG ATCCGGAGAA CAGGATGTGG 
ACCAGTGAGG GCGAGTCTGA CAAGAAGACC ATCTTCGCCT TCGTGGCCAG GAGGTCGCAC 
AGCTCCACGG ACAACCAGTG CCATGTGTTC TGCGACCTGT CCGTCAGCCA GCCGGCGGCG 
GCGATTGTCT CGTTCGCCAA TCGGACACTG CCCACCGAAA AGCTGCGCAA CTACGTCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163570.1
CDS	28..2190
Translation	MRTPYEESAAAADHKYQYQYTAANNNNNYYKNYNVNGNGQANGNGNLSGIGNGNGIGNGNGYGIPYHARENSLPFSYGIAKTSTPLRKTPTPTRVDSFLDFDFGPTNTNVNSNSSSNNGGTGDMIKPPTRLTSPLLVRKTLGNAVSPTSSTNTNTIANTYHYTSSSATPTPVRDNFEQLLRERREKVYNEYRNLDRESSGERARVAPAPPQRQYYPSDGYGIGLKNDSNGTLYRKSPSSNGKSTKYNYDFEFNLNLDDVRPEPTPYTTRNIHFEDLRLDQDVPDGVVNRRTMERNYHTINSLETTHKRQQLEPLEQLGAVYGQNHMEETLTRTRRDLSASTSTSKSTSPLPAIGVPAIAPASPIYATSTKRVSNPNTNGQQQMTPSPTSRPETPAFPVTPRTPFGASSSSSASLAPTTQLPPESIYQTGPRRGVPPALQPLEVSADSVQFARSSSQFWYKPNLTREDAIALLASAQPGTFLVRDSTTYKDSYGLVVRVSQPPPGSQELVRHFLIEPTKGGVHLKGCDDEPVFTSLSALVFEHSISQLALPCLLRLPDRDIVPPVRATTPAQQHLLAHGAATNVLWLYSCDTESLTGNEAIRKAIRQMYGQQPLPQPTEVHFKVSSQGITLTDNTRKKFFRKHYKADVISHCAIDPENRMWTSEGESDKKTIFAFVARRSHSSTDNQCHVFCDLSVSQPAAAIVSFANRTLPTEKLRNYVL

Target ORF information:

RefSeq Version	NM_001170099.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster blistery (by), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170099.3

ORF Insert Sequence:

ATGCGGACGC CGTACGAAGA AAGCGCTGCT GCCGCCGACC ACAAATATCA ATACCAATAC 
ACCGCGGCCA ATAATAACAA TAATTACTAT AAAAACTATA ATGTGAATGG CAATGGGCAG 
GCGAATGGAA ATGGGAATCT AAGTGGAATT GGTAATGGAA ATGGAATTGG AAACGGCAAT 
GGATATGGAA TACCTTATCA CGCGCGCGAG AACTCGCTGC CATTTAGCTA TGGCATAGCC 
AAAACTTCGA CACCGTTGCG AAAAACTCCG ACACCGACGC GAGTGGATAG TTTTCTAGAC 
TTTGATTTCG GGCCAACGAA TACGAATGTG AATTCGAATA GTTCTAGCAA TAATGGCGGT 
ACTGGCGATA TGATAAAGCC ACCAACACGT CTAACGAGCC CTTTGCTGGT GCGCAAAACG 
CTGGGCAATG CCGTTAGTCC CACCAGTTCG ACCAATACCA ACACCATAGC CAATACATAC 
CACTACACGT CGAGCAGCGC AACGCCGACG CCGGTGAGAG ATAATTTCGA GCAGCTGCTG 
CGGGAGCGAC GCGAAAAGGT CTACAATGAA TATAGGAATC TGGATAGGGA GTCGTCCGGG 
GAGCGGGCCA GAGTGGCACC CGCTCCGCCG CAGAGGCAAT ACTACCCGAG CGATGGCTAT 
GGAATTGGGC TGAAGAACGA TAGCAATGGA ACCCTATACC GCAAGAGCCC CTCGTCTAAT 
GGGAAATCAA CGAAATACAA CTACGATTTT GAGTTCAATC TGAATCTGGA TGATGTTAGG 
CCCGAACCTA CACCGTATAC CACGAGGAAT ATTCACTTCG AGGATCTGCG GCTGGATCAG 
GATGTGCCAG ATGGCGTGGT CAATCGGCGG ACCATGGAAC GCAATTATCA CACAATTAAC 
AGCTTGGAAA CGACCCACAA ACGCCAGCAG TTGGAGCCGT TGGAGCAACT GGGCGCCGTT 
TATGGCCAGA ACCACATGGA GGAGACACTC ACGCGCACTC GTCGCGATCT CTCCGCATCC 
ACATCGACAT CCAAGTCCAC CTCGCCATTG CCGGCAATAG GAGTACCAGC TATAGCACCA 
GCTTCGCCCA TTTATGCCAC CAGTACGAAG AGAGTGAGCA ACCCAAATAC CAATGGCCAA 
CAGCAGATGA CGCCTAGTCC CACCAGTAGG CCAGAAACGC CCGCCTTTCC GGTCACGCCG 
CGCACGCCTT TTGGGGCATC ATCATCATCA TCTGCGTCTC TGGCGCCGAC GACCCAGTTG 
CCCCCAGAGT CGATCTACCA AACAGGGCCA CGTCGTGGCG TACCGCCTGC ACTGCAGCCA 
CTCGAGGTGT CCGCCGATTC CGTACAATTT GCCCGTAGTT CGTCCCAGTT CTGGTACAAA 
CCAAATCTCA CTCGCGAAGA TGCCATCGCT CTGCTGGCCT CTGCCCAGCC GGGCACGTTC 
CTGGTCAGGG ACTCCACCAC GTACAAGGAT TCCTATGGCC TGGTGGTGCG GGTGTCCCAA 
CCGCCTCCCG GTTCACAGGA GCTGGTGCGT CACTTCCTCA TCGAGCCCAC GAAGGGGGGA 
GTGCATCTGA AGGGCTGCGA TGACGAGCCG GTGTTCACTT CACTGTCCGC CCTCGTCTTC 
GAGCATTCCA TAAGCCAGCT GGCGTTGCCA TGTCTCCTCC GTCTGCCCGA TCGGGATATA 
GTACCACCGG TGCGGGCCAC AACCCCTGCC CAGCAGCATC TATTGGCCCA CGGAGCAGCC 
ACCAATGTGC TGTGGCTCTA CTCCTGCGAC ACGGAATCGC TGACAGGAAA CGAGGCCATC 
CGCAAGGCCA TCCGCCAGAT GTACGGTCAG CAACCCTTGC CGCAGCCCAC GGAGGTGCAC 
TTCAAGGTCT CCTCGCAGGG CATCACGCTC ACGGACAATA CGCGCAAGAA GTTCTTCCGG 
AAGCACTACA AGGCGGATGT TATCTCGCAC TGTGCCATCG ATCCGGAGAA CAGGATGTGG 
ACCAGTGAGG GCGAGTCTGA CAAGAAGACC ATCTTCGCCT TCGTGGCCAG GAGGTCGCAC 
AGCTCCACGG ACAACCAGTG CCATGTGTTC TGCGACCTGT CCGTCAGCCA GCCGGCGGCG 
GCGATTGTCT CGTTCGCCAA TCGGACACTG CCCACCGAAA AGCTGCGCAA CTACGTCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

by ( NM_141644.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649901.1 Drosophila melanogaster blistery (by), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa04333
Clone ID Related Accession (Same CDS sequence)	NM_001170099.3 , NM_141644.4 , NM_001170098.2
Accession Version	NM_141644.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	blistery, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141644.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGGACGC CGTACGAAGA AAGCGCTGCT GCCGCCGACC ACAAATATCA ATACCAATAC 
ACCGCGGCCA ATAATAACAA TAATTACTAT AAAAACTATA ATGTGAATGG CAATGGGCAG 
GCGAATGGAA ATGGGAATCT AAGTGGAATT GGTAATGGAA ATGGAATTGG AAACGGCAAT 
GGATATGGAA TACCTTATCA CGCGCGCGAG AACTCGCTGC CATTTAGCTA TGGCATAGCC 
AAAACTTCGA CACCGTTGCG AAAAACTCCG ACACCGACGC GAGTGGATAG TTTTCTAGAC 
TTTGATTTCG GGCCAACGAA TACGAATGTG AATTCGAATA GTTCTAGCAA TAATGGCGGT 
ACTGGCGATA TGATAAAGCC ACCAACACGT CTAACGAGCC CTTTGCTGGT GCGCAAAACG 
CTGGGCAATG CCGTTAGTCC CACCAGTTCG ACCAATACCA ACACCATAGC CAATACATAC 
CACTACACGT CGAGCAGCGC AACGCCGACG CCGGTGAGAG ATAATTTCGA GCAGCTGCTG 
CGGGAGCGAC GCGAAAAGGT CTACAATGAA TATAGGAATC TGGATAGGGA GTCGTCCGGG 
GAGCGGGCCA GAGTGGCACC CGCTCCGCCG CAGAGGCAAT ACTACCCGAG CGATGGCTAT 
GGAATTGGGC TGAAGAACGA TAGCAATGGA ACCCTATACC GCAAGAGCCC CTCGTCTAAT 
GGGAAATCAA CGAAATACAA CTACGATTTT GAGTTCAATC TGAATCTGGA TGATGTTAGG 
CCCGAACCTA CACCGTATAC CACGAGGAAT ATTCACTTCG AGGATCTGCG GCTGGATCAG 
GATGTGCCAG ATGGCGTGGT CAATCGGCGG ACCATGGAAC GCAATTATCA CACAATTAAC 
AGCTTGGAAA CGACCCACAA ACGCCAGCAG TTGGAGCCGT TGGAGCAACT GGGCGCCGTT 
TATGGCCAGA ACCACATGGA GGAGACACTC ACGCGCACTC GTCGCGATCT CTCCGCATCC 
ACATCGACAT CCAAGTCCAC CTCGCCATTG CCGGCAATAG GAGTACCAGC TATAGCACCA 
GCTTCGCCCA TTTATGCCAC CAGTACGAAG AGAGTGAGCA ACCCAAATAC CAATGGCCAA 
CAGCAGATGA CGCCTAGTCC CACCAGTAGG CCAGAAACGC CCGCCTTTCC GGTCACGCCG 
CGCACGCCTT TTGGGGCATC ATCATCATCA TCTGCGTCTC TGGCGCCGAC GACCCAGTTG 
CCCCCAGAGT CGATCTACCA AACAGGGCCA CGTCGTGGCG TACCGCCTGC ACTGCAGCCA 
CTCGAGGTGT CCGCCGATTC CGTACAATTT GCCCGTAGTT CGTCCCAGTT CTGGTACAAA 
CCAAATCTCA CTCGCGAAGA TGCCATCGCT CTGCTGGCCT CTGCCCAGCC GGGCACGTTC 
CTGGTCAGGG ACTCCACCAC GTACAAGGAT TCCTATGGCC TGGTGGTGCG GGTGTCCCAA 
CCGCCTCCCG GTTCACAGGA GCTGGTGCGT CACTTCCTCA TCGAGCCCAC GAAGGGGGGA 
GTGCATCTGA AGGGCTGCGA TGACGAGCCG GTGTTCACTT CACTGTCCGC CCTCGTCTTC 
GAGCATTCCA TAAGCCAGCT GGCGTTGCCA TGTCTCCTCC GTCTGCCCGA TCGGGATATA 
GTACCACCGG TGCGGGCCAC AACCCCTGCC CAGCAGCATC TATTGGCCCA CGGAGCAGCC 
ACCAATGTGC TGTGGCTCTA CTCCTGCGAC ACGGAATCGC TGACAGGAAA CGAGGCCATC 
CGCAAGGCCA TCCGCCAGAT GTACGGTCAG CAACCCTTGC CGCAGCCCAC GGAGGTGCAC 
TTCAAGGTCT CCTCGCAGGG CATCACGCTC ACGGACAATA CGCGCAAGAA GTTCTTCCGG 
AAGCACTACA AGGCGGATGT TATCTCGCAC TGTGCCATCG ATCCGGAGAA CAGGATGTGG 
ACCAGTGAGG GCGAGTCTGA CAAGAAGACC ATCTTCGCCT TCGTGGCCAG GAGGTCGCAC 
AGCTCCACGG ACAACCAGTG CCATGTGTTC TGCGACCTGT CCGTCAGCCA GCCGGCGGCG 
GCGATTGTCT CGTTCGCCAA TCGGACACTG CCCACCGAAA AGCTGCGCAA CTACGTCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649901.1
CDS	28..2190
Translation	MRTPYEESAAAADHKYQYQYTAANNNNNYYKNYNVNGNGQANGNGNLSGIGNGNGIGNGNGYGIPYHARENSLPFSYGIAKTSTPLRKTPTPTRVDSFLDFDFGPTNTNVNSNSSSNNGGTGDMIKPPTRLTSPLLVRKTLGNAVSPTSSTNTNTIANTYHYTSSSATPTPVRDNFEQLLRERREKVYNEYRNLDRESSGERARVAPAPPQRQYYPSDGYGIGLKNDSNGTLYRKSPSSNGKSTKYNYDFEFNLNLDDVRPEPTPYTTRNIHFEDLRLDQDVPDGVVNRRTMERNYHTINSLETTHKRQQLEPLEQLGAVYGQNHMEETLTRTRRDLSASTSTSKSTSPLPAIGVPAIAPASPIYATSTKRVSNPNTNGQQQMTPSPTSRPETPAFPVTPRTPFGASSSSSASLAPTTQLPPESIYQTGPRRGVPPALQPLEVSADSVQFARSSSQFWYKPNLTREDAIALLASAQPGTFLVRDSTTYKDSYGLVVRVSQPPPGSQELVRHFLIEPTKGGVHLKGCDDEPVFTSLSALVFEHSISQLALPCLLRLPDRDIVPPVRATTPAQQHLLAHGAATNVLWLYSCDTESLTGNEAIRKAIRQMYGQQPLPQPTEVHFKVSSQGITLTDNTRKKFFRKHYKADVISHCAIDPENRMWTSEGESDKKTIFAFVARRSHSSTDNQCHVFCDLSVSQPAAAIVSFANRTLPTEKLRNYVL

Target ORF information:

RefSeq Version	NM_141644.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster blistery (by), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141644.4

ORF Insert Sequence:

ATGCGGACGC CGTACGAAGA AAGCGCTGCT GCCGCCGACC ACAAATATCA ATACCAATAC 
ACCGCGGCCA ATAATAACAA TAATTACTAT AAAAACTATA ATGTGAATGG CAATGGGCAG 
GCGAATGGAA ATGGGAATCT AAGTGGAATT GGTAATGGAA ATGGAATTGG AAACGGCAAT 
GGATATGGAA TACCTTATCA CGCGCGCGAG AACTCGCTGC CATTTAGCTA TGGCATAGCC 
AAAACTTCGA CACCGTTGCG AAAAACTCCG ACACCGACGC GAGTGGATAG TTTTCTAGAC 
TTTGATTTCG GGCCAACGAA TACGAATGTG AATTCGAATA GTTCTAGCAA TAATGGCGGT 
ACTGGCGATA TGATAAAGCC ACCAACACGT CTAACGAGCC CTTTGCTGGT GCGCAAAACG 
CTGGGCAATG CCGTTAGTCC CACCAGTTCG ACCAATACCA ACACCATAGC CAATACATAC 
CACTACACGT CGAGCAGCGC AACGCCGACG CCGGTGAGAG ATAATTTCGA GCAGCTGCTG 
CGGGAGCGAC GCGAAAAGGT CTACAATGAA TATAGGAATC TGGATAGGGA GTCGTCCGGG 
GAGCGGGCCA GAGTGGCACC CGCTCCGCCG CAGAGGCAAT ACTACCCGAG CGATGGCTAT 
GGAATTGGGC TGAAGAACGA TAGCAATGGA ACCCTATACC GCAAGAGCCC CTCGTCTAAT 
GGGAAATCAA CGAAATACAA CTACGATTTT GAGTTCAATC TGAATCTGGA TGATGTTAGG 
CCCGAACCTA CACCGTATAC CACGAGGAAT ATTCACTTCG AGGATCTGCG GCTGGATCAG 
GATGTGCCAG ATGGCGTGGT CAATCGGCGG ACCATGGAAC GCAATTATCA CACAATTAAC 
AGCTTGGAAA CGACCCACAA ACGCCAGCAG TTGGAGCCGT TGGAGCAACT GGGCGCCGTT 
TATGGCCAGA ACCACATGGA GGAGACACTC ACGCGCACTC GTCGCGATCT CTCCGCATCC 
ACATCGACAT CCAAGTCCAC CTCGCCATTG CCGGCAATAG GAGTACCAGC TATAGCACCA 
GCTTCGCCCA TTTATGCCAC CAGTACGAAG AGAGTGAGCA ACCCAAATAC CAATGGCCAA 
CAGCAGATGA CGCCTAGTCC CACCAGTAGG CCAGAAACGC CCGCCTTTCC GGTCACGCCG 
CGCACGCCTT TTGGGGCATC ATCATCATCA TCTGCGTCTC TGGCGCCGAC GACCCAGTTG 
CCCCCAGAGT CGATCTACCA AACAGGGCCA CGTCGTGGCG TACCGCCTGC ACTGCAGCCA 
CTCGAGGTGT CCGCCGATTC CGTACAATTT GCCCGTAGTT CGTCCCAGTT CTGGTACAAA 
CCAAATCTCA CTCGCGAAGA TGCCATCGCT CTGCTGGCCT CTGCCCAGCC GGGCACGTTC 
CTGGTCAGGG ACTCCACCAC GTACAAGGAT TCCTATGGCC TGGTGGTGCG GGTGTCCCAA 
CCGCCTCCCG GTTCACAGGA GCTGGTGCGT CACTTCCTCA TCGAGCCCAC GAAGGGGGGA 
GTGCATCTGA AGGGCTGCGA TGACGAGCCG GTGTTCACTT CACTGTCCGC CCTCGTCTTC 
GAGCATTCCA TAAGCCAGCT GGCGTTGCCA TGTCTCCTCC GTCTGCCCGA TCGGGATATA 
GTACCACCGG TGCGGGCCAC AACCCCTGCC CAGCAGCATC TATTGGCCCA CGGAGCAGCC 
ACCAATGTGC TGTGGCTCTA CTCCTGCGAC ACGGAATCGC TGACAGGAAA CGAGGCCATC 
CGCAAGGCCA TCCGCCAGAT GTACGGTCAG CAACCCTTGC CGCAGCCCAC GGAGGTGCAC 
TTCAAGGTCT CCTCGCAGGG CATCACGCTC ACGGACAATA CGCGCAAGAA GTTCTTCCGG 
AAGCACTACA AGGCGGATGT TATCTCGCAC TGTGCCATCG ATCCGGAGAA CAGGATGTGG 
ACCAGTGAGG GCGAGTCTGA CAAGAAGACC ATCTTCGCCT TCGTGGCCAG GAGGTCGCAC 
AGCTCCACGG ACAACCAGTG CCATGTGTTC TGCGACCTGT CCGTCAGCCA GCCGGCGGCG 
GCGATTGTCT CGTTCGCCAA TCGGACACTG CCCACCGAAA AGCTGCGCAA CTACGTCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

by ( NM_001170098.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163569.1 Drosophila melanogaster blistery (by), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa04333
Clone ID Related Accession (Same CDS sequence)	NM_001170099.3 , NM_141644.4 , NM_001170098.2
Accession Version	NM_001170098.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	blistery, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170098.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGGACGC CGTACGAAGA AAGCGCTGCT GCCGCCGACC ACAAATATCA ATACCAATAC 
ACCGCGGCCA ATAATAACAA TAATTACTAT AAAAACTATA ATGTGAATGG CAATGGGCAG 
GCGAATGGAA ATGGGAATCT AAGTGGAATT GGTAATGGAA ATGGAATTGG AAACGGCAAT 
GGATATGGAA TACCTTATCA CGCGCGCGAG AACTCGCTGC CATTTAGCTA TGGCATAGCC 
AAAACTTCGA CACCGTTGCG AAAAACTCCG ACACCGACGC GAGTGGATAG TTTTCTAGAC 
TTTGATTTCG GGCCAACGAA TACGAATGTG AATTCGAATA GTTCTAGCAA TAATGGCGGT 
ACTGGCGATA TGATAAAGCC ACCAACACGT CTAACGAGCC CTTTGCTGGT GCGCAAAACG 
CTGGGCAATG CCGTTAGTCC CACCAGTTCG ACCAATACCA ACACCATAGC CAATACATAC 
CACTACACGT CGAGCAGCGC AACGCCGACG CCGGTGAGAG ATAATTTCGA GCAGCTGCTG 
CGGGAGCGAC GCGAAAAGGT CTACAATGAA TATAGGAATC TGGATAGGGA GTCGTCCGGG 
GAGCGGGCCA GAGTGGCACC CGCTCCGCCG CAGAGGCAAT ACTACCCGAG CGATGGCTAT 
GGAATTGGGC TGAAGAACGA TAGCAATGGA ACCCTATACC GCAAGAGCCC CTCGTCTAAT 
GGGAAATCAA CGAAATACAA CTACGATTTT GAGTTCAATC TGAATCTGGA TGATGTTAGG 
CCCGAACCTA CACCGTATAC CACGAGGAAT ATTCACTTCG AGGATCTGCG GCTGGATCAG 
GATGTGCCAG ATGGCGTGGT CAATCGGCGG ACCATGGAAC GCAATTATCA CACAATTAAC 
AGCTTGGAAA CGACCCACAA ACGCCAGCAG TTGGAGCCGT TGGAGCAACT GGGCGCCGTT 
TATGGCCAGA ACCACATGGA GGAGACACTC ACGCGCACTC GTCGCGATCT CTCCGCATCC 
ACATCGACAT CCAAGTCCAC CTCGCCATTG CCGGCAATAG GAGTACCAGC TATAGCACCA 
GCTTCGCCCA TTTATGCCAC CAGTACGAAG AGAGTGAGCA ACCCAAATAC CAATGGCCAA 
CAGCAGATGA CGCCTAGTCC CACCAGTAGG CCAGAAACGC CCGCCTTTCC GGTCACGCCG 
CGCACGCCTT TTGGGGCATC ATCATCATCA TCTGCGTCTC TGGCGCCGAC GACCCAGTTG 
CCCCCAGAGT CGATCTACCA AACAGGGCCA CGTCGTGGCG TACCGCCTGC ACTGCAGCCA 
CTCGAGGTGT CCGCCGATTC CGTACAATTT GCCCGTAGTT CGTCCCAGTT CTGGTACAAA 
CCAAATCTCA CTCGCGAAGA TGCCATCGCT CTGCTGGCCT CTGCCCAGCC GGGCACGTTC 
CTGGTCAGGG ACTCCACCAC GTACAAGGAT TCCTATGGCC TGGTGGTGCG GGTGTCCCAA 
CCGCCTCCCG GTTCACAGGA GCTGGTGCGT CACTTCCTCA TCGAGCCCAC GAAGGGGGGA 
GTGCATCTGA AGGGCTGCGA TGACGAGCCG GTGTTCACTT CACTGTCCGC CCTCGTCTTC 
GAGCATTCCA TAAGCCAGCT GGCGTTGCCA TGTCTCCTCC GTCTGCCCGA TCGGGATATA 
GTACCACCGG TGCGGGCCAC AACCCCTGCC CAGCAGCATC TATTGGCCCA CGGAGCAGCC 
ACCAATGTGC TGTGGCTCTA CTCCTGCGAC ACGGAATCGC TGACAGGAAA CGAGGCCATC 
CGCAAGGCCA TCCGCCAGAT GTACGGTCAG CAACCCTTGC CGCAGCCCAC GGAGGTGCAC 
TTCAAGGTCT CCTCGCAGGG CATCACGCTC ACGGACAATA CGCGCAAGAA GTTCTTCCGG 
AAGCACTACA AGGCGGATGT TATCTCGCAC TGTGCCATCG ATCCGGAGAA CAGGATGTGG 
ACCAGTGAGG GCGAGTCTGA CAAGAAGACC ATCTTCGCCT TCGTGGCCAG GAGGTCGCAC 
AGCTCCACGG ACAACCAGTG CCATGTGTTC TGCGACCTGT CCGTCAGCCA GCCGGCGGCG 
GCGATTGTCT CGTTCGCCAA TCGGACACTG CCCACCGAAA AGCTGCGCAA CTACGTCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163569.1
CDS	28..2190
Translation	MRTPYEESAAAADHKYQYQYTAANNNNNYYKNYNVNGNGQANGNGNLSGIGNGNGIGNGNGYGIPYHARENSLPFSYGIAKTSTPLRKTPTPTRVDSFLDFDFGPTNTNVNSNSSSNNGGTGDMIKPPTRLTSPLLVRKTLGNAVSPTSSTNTNTIANTYHYTSSSATPTPVRDNFEQLLRERREKVYNEYRNLDRESSGERARVAPAPPQRQYYPSDGYGIGLKNDSNGTLYRKSPSSNGKSTKYNYDFEFNLNLDDVRPEPTPYTTRNIHFEDLRLDQDVPDGVVNRRTMERNYHTINSLETTHKRQQLEPLEQLGAVYGQNHMEETLTRTRRDLSASTSTSKSTSPLPAIGVPAIAPASPIYATSTKRVSNPNTNGQQQMTPSPTSRPETPAFPVTPRTPFGASSSSSASLAPTTQLPPESIYQTGPRRGVPPALQPLEVSADSVQFARSSSQFWYKPNLTREDAIALLASAQPGTFLVRDSTTYKDSYGLVVRVSQPPPGSQELVRHFLIEPTKGGVHLKGCDDEPVFTSLSALVFEHSISQLALPCLLRLPDRDIVPPVRATTPAQQHLLAHGAATNVLWLYSCDTESLTGNEAIRKAIRQMYGQQPLPQPTEVHFKVSSQGITLTDNTRKKFFRKHYKADVISHCAIDPENRMWTSEGESDKKTIFAFVARRSHSSTDNQCHVFCDLSVSQPAAAIVSFANRTLPTEKLRNYVL

Target ORF information:

RefSeq Version	NM_001170098.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster blistery (by), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170098.2

ORF Insert Sequence:

ATGCGGACGC CGTACGAAGA AAGCGCTGCT GCCGCCGACC ACAAATATCA ATACCAATAC 
ACCGCGGCCA ATAATAACAA TAATTACTAT AAAAACTATA ATGTGAATGG CAATGGGCAG 
GCGAATGGAA ATGGGAATCT AAGTGGAATT GGTAATGGAA ATGGAATTGG AAACGGCAAT 
GGATATGGAA TACCTTATCA CGCGCGCGAG AACTCGCTGC CATTTAGCTA TGGCATAGCC 
AAAACTTCGA CACCGTTGCG AAAAACTCCG ACACCGACGC GAGTGGATAG TTTTCTAGAC 
TTTGATTTCG GGCCAACGAA TACGAATGTG AATTCGAATA GTTCTAGCAA TAATGGCGGT 
ACTGGCGATA TGATAAAGCC ACCAACACGT CTAACGAGCC CTTTGCTGGT GCGCAAAACG 
CTGGGCAATG CCGTTAGTCC CACCAGTTCG ACCAATACCA ACACCATAGC CAATACATAC 
CACTACACGT CGAGCAGCGC AACGCCGACG CCGGTGAGAG ATAATTTCGA GCAGCTGCTG 
CGGGAGCGAC GCGAAAAGGT CTACAATGAA TATAGGAATC TGGATAGGGA GTCGTCCGGG 
GAGCGGGCCA GAGTGGCACC CGCTCCGCCG CAGAGGCAAT ACTACCCGAG CGATGGCTAT 
GGAATTGGGC TGAAGAACGA TAGCAATGGA ACCCTATACC GCAAGAGCCC CTCGTCTAAT 
GGGAAATCAA CGAAATACAA CTACGATTTT GAGTTCAATC TGAATCTGGA TGATGTTAGG 
CCCGAACCTA CACCGTATAC CACGAGGAAT ATTCACTTCG AGGATCTGCG GCTGGATCAG 
GATGTGCCAG ATGGCGTGGT CAATCGGCGG ACCATGGAAC GCAATTATCA CACAATTAAC 
AGCTTGGAAA CGACCCACAA ACGCCAGCAG TTGGAGCCGT TGGAGCAACT GGGCGCCGTT 
TATGGCCAGA ACCACATGGA GGAGACACTC ACGCGCACTC GTCGCGATCT CTCCGCATCC 
ACATCGACAT CCAAGTCCAC CTCGCCATTG CCGGCAATAG GAGTACCAGC TATAGCACCA 
GCTTCGCCCA TTTATGCCAC CAGTACGAAG AGAGTGAGCA ACCCAAATAC CAATGGCCAA 
CAGCAGATGA CGCCTAGTCC CACCAGTAGG CCAGAAACGC CCGCCTTTCC GGTCACGCCG 
CGCACGCCTT TTGGGGCATC ATCATCATCA TCTGCGTCTC TGGCGCCGAC GACCCAGTTG 
CCCCCAGAGT CGATCTACCA AACAGGGCCA CGTCGTGGCG TACCGCCTGC ACTGCAGCCA 
CTCGAGGTGT CCGCCGATTC CGTACAATTT GCCCGTAGTT CGTCCCAGTT CTGGTACAAA 
CCAAATCTCA CTCGCGAAGA TGCCATCGCT CTGCTGGCCT CTGCCCAGCC GGGCACGTTC 
CTGGTCAGGG ACTCCACCAC GTACAAGGAT TCCTATGGCC TGGTGGTGCG GGTGTCCCAA 
CCGCCTCCCG GTTCACAGGA GCTGGTGCGT CACTTCCTCA TCGAGCCCAC GAAGGGGGGA 
GTGCATCTGA AGGGCTGCGA TGACGAGCCG GTGTTCACTT CACTGTCCGC CCTCGTCTTC 
GAGCATTCCA TAAGCCAGCT GGCGTTGCCA TGTCTCCTCC GTCTGCCCGA TCGGGATATA 
GTACCACCGG TGCGGGCCAC AACCCCTGCC CAGCAGCATC TATTGGCCCA CGGAGCAGCC 
ACCAATGTGC TGTGGCTCTA CTCCTGCGAC ACGGAATCGC TGACAGGAAA CGAGGCCATC 
CGCAAGGCCA TCCGCCAGAT GTACGGTCAG CAACCCTTGC CGCAGCCCAC GGAGGTGCAC 
TTCAAGGTCT CCTCGCAGGG CATCACGCTC ACGGACAATA CGCGCAAGAA GTTCTTCCGG 
AAGCACTACA AGGCGGATGT TATCTCGCAC TGTGCCATCG ATCCGGAGAA CAGGATGTGG 
ACCAGTGAGG GCGAGTCTGA CAAGAAGACC ATCTTCGCCT TCGTGGCCAG GAGGTCGCAC 
AGCTCCACGG ACAACCAGTG CCATGTGTTC TGCGACCTGT CCGTCAGCCA GCCGGCGGCG 
GCGATTGTCT CGTTCGCCAA TCGGACACTG CCCACCGAAA AGCTGCGCAA CTACGTCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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by cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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