Vps15 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vps15
Entrez Gene ID	41096
Full Name	Vacuolar protein sorting 15
Synonyms	CG9746,Dmel\CG9746,Ird1,dVps15,dVps15/p150,ird,ird1,vps15
General protein information	Preferred Names Vacuolar protein sorting 15 Names CG9746 gene product from transcript CG9746-RA CG9746-PA CG9746-PB CG9746-PC Vps15-PA Vps15-PB Vps15-PC immune response deficient 1 immune response-deficient 1 vacuolar protein sorting 15
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 85D1-85D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_141611.2	NP_649868.1	vacuolar protein sorting 15, isoform A
NM_001170094.2	NP_001163565.1	vacuolar protein sorting 15, isoform B
NM_001275476.1	NP_001262405.1	vacuolar protein sorting 15, isoform C

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PIK3R4	XP_004939498.1
Danio rerio (zebrafish)	pik3r4	XP_001922676.1
Oryza sativa (rice)	Os02g0796700	NP_001048391.1
Canis lupus familiaris (dog)	PIK3R4	XP_534260.1
Bos taurus (cattle)	PIK3R4	NP_001093784.1
Eremothecium gossypii	AGOS_ADL316C	NP_983780.2
Magnaporthe oryzae (rice blast fungus)	MGG_06100	XP_003711966.1
Neurospora crassa	NCU06626	XP_962368.2
	PIK3R4	XP_002802990.1
Mus musculus (house mouse)	Pik3r4	NP_001074778.1
Rattus norvegicus (Norway rat)	Pik3r4	NP_001102247.1
Saccharomyces cerevisiae (baker's yeast)	VPS15	NP_009655.2
Schizosaccharomyces pombe (fission yeast)	ppk19	NP_595288.1
Xenopus tropicalis (tropical clawed frog)	pik3r4	NP_001107701.1
Pan troglodytes (chimpanzee)	PIK3R4	XP_516746.2
Drosophila melanogaster (fruit fly)	ird1	NP_649868.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000602	XP_310476.5
Arabidopsis thaliana (thale cress)	AT4G29380	NP_194667.1
Homo sapiens (human)	PIK3R4	NP_055417.1
Kluyveromyces lactis	KLLA0B11924g	XP_452060.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results provide the first genetic evidence for a function of Vps15 in autophagy in multicellular organisms and suggest that the Vps15-containing PI 3-kinase complex may play an important role in clearance of protein aggregates
Title: The PI 3-kinase regulator Vps15 is required for autophagic clearance of protein aggregates.

The following Vps15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vps15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04320	NM_001275476.1 Latest version!	Drosophila melanogaster vacuolar protein sorting 15, transcript variant C (Vps15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa04321	NM_001170094.2 Latest version!	Drosophila melanogaster vacuolar protein sorting 15, transcript variant B (Vps15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa08174	NM_141611.2 Latest version!	Drosophila melanogaster vacuolar protein sorting 15, transcript variant A (Vps15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vps15 ( NM_001275476.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262405.1 Drosophila melanogaster vacuolar protein sorting 15, transcript variant C (Vps15), mRNA.

Price & Availability↑

CloneID	OFa04320
Clone ID Related Accession (Same CDS sequence)	NM_001275476.1
Accession Version	NM_001275476.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar protein sorting 15, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAATC AACTGGTGGG CATTGCCCCT TCGCAAATTT ATGCGGTGGA ACACTATTTT 
TCCGGCCAGT TTGGATCCGA GATCGCCTTC AGTTCCAATA TGGGCAGCAC GAGATTCTTC 
AAGGTGGCCA AGGCTAAAAC GGATGAGGGG CAAATAGTGG TCAAGGTGTT TGTGAAGCAC 
GATCCCACTT TGCCACTGGA GGATCACAAG GAGCGATTGG AGGGCATCAA GAAGACACTC 
TCGCTGGCCA ATGCCGTCAA TTGTTTACCC TTTCAGCGGG TCGAGCTTAT CGACAAGGCA 
GCCTACATAA TGCGGGAGTA TGTGAAGCAC AGTCTCTATG ATCGCGTATC CACACGACCA 
TTTCTCACCG TATTGGAGAA AAAGTGGATA ACGTTCCAAA TTTTGTGTGC CCTGAATCAG 
TGCCACAAAC AGAAGATCTG CCACGGGGAC ATCAAGCTGG AGAACATCTT GATTACGTCT 
TGGAACTGGA TTCTGCTCTC CGACTTTGCC TCCTTTAAGC CCACATATTT GCCGGAGGAC 
AATCCGGCGG ATTATACGTA CTTCTTCGAT ACGAGTAGAA GGCGAACCTG CTATATTGCA 
CCCGAACGAT TCGTTAAAAC ACTGGCCTCC GATGATGATG GTGGCAATGG TAATATGTCG 
GTCATACACA CCGATTCCAT AATTCGACTT GCTCCTAGCT ATGCGGGTAA CACTTTGTTG 
CCAGCCATGG ACATCTTTTC GGCAGGCTGT GCCTTGCTGG AACTCTGGAC AGAGGGAACT 
GCGCCCTTTG AACTCTCCCA GCTGCTGGCT TACAGGCGTG GCGAGCGAGA TCTGGTGGAG 
AAACATCTGG CGGGCATCGA AAACGAAAGG TTGCGCAATC TGCTGGCCTC TATGATTGAC 
ATACACTCGA TGAACCGAAA GAGCGCCGAG GATTACTTGG ACCAGGAGCG CGGTCAGTTG 
TTTCCCGAAT ACTTCTACTC CTTCCTGCAG TCGTACTTGC AAATGTTCAG CTCAACACCG 
ATCATGTCGC CGGATGACAA GATTCAGTGT CTGCATAAGG ACATTGGGCA CTGTATTAAA 
GTGCTGACAC AGGCCCAGGA TTTAACACCG GAAGAGGAGG AAAAGGAAGA AAAGAAGGAT 
CCCACGTTGT GTGCCCGACT GCCACCGGAG CATGATGGTC TCATTTTGAT CATCACGGTA 
GTCACATCCT GCATACGAGG ACTGAAGCAG TCGAATACCA AAATTGCAGC CTTGGAACTT 
CTGCAAAAGC TGTCGAAGTA CACAACATCC GAGACCATCC TGGATCGCAT TTTGCCCTAC 
ATTCTCCATT TGGCCCAGAA ATCGCCAGCC AAGGTGCAGG TTCAGGCCAT CGAAACTCTG 
ACCGCCTGTT TGGGCATGGT AAAGGACATT CCACGCAGCG ATGCCAACGT GTTTCCCGAG 
TACATTCTGC CCTCCATCAC CGATTTGGCC AGCGAGACGA GTGCAGTTAT TGTTCGCGTG 
GCCTTTGCCC GGAATATAGC TGCACTGGCC AAGAGCGCTG TCTACTTTTT GGAGGAAACG 
CAACGAAATG CTCCTAACGA TATGCCCACT CCACGTTACG AGGCAGAACT GAACGCCCTC 
CATGACATAG TTAGACAGGC AGTACTCAGT CTGTTAACGG ATTCCCAACC GGTGGTCAAG 
CAAACACTGA TGGAGTCGGG CATCTGCGAT CTCTGTGCCT TCTTTGGCAA GGAAAAAGCG 
AACGATGTGA TACTCTCGCA CATCATGACG TTCCTTAATG ATGAGGACAA GAACTTGAGA 
GGAGCATTCT ATGACAACAT CGCCGGAGTG GCTGGCTATG TGGGCTGGCA GGCGTCGGAC 
ATTCTCGTGC CACTTCTGCA GCAAGGTTTC ACGGATCGCG AGGAGTTCGT CATTGCAAAA 
GCCATTCGTG CTGTGACCAT TCTTATCGAA CTGGGTCACA TTAAGAAGCC AGCCGTTACG 
GAAATTGTCC AAGAGACAGC CTGTTATCTG TGCCATCCAA ATCTTTGGGT TCGTCACGAA 
ATCTGCGGCA TGATTGCCAC CACGGCGCGT AATCTCAGCG CCATCGATGT GCAGTGCAAG 
ATTATGCCGG CGATTGGGGC ATTTCTCAAG GCCCCGCTCA TCCAGGTGGA GAAACCGCAC 
ATCCTGTTGG ACTGTGTGCA TCCGCCTGTG CCGCGGCAGA TCTTCGACAG CGTTCTGCGC 
TTCCAAGACA TTCATCATTT TATCAGAGCA CTGGAGGCAC GATCACGTGT TCGTAGTCAG 
ACGAGGCAGG GAGCTCTGCC GCAGTACGAG GAGATGGGAC AGACCTTGCG TAATCTCTTT 
AGACGCTTAA GCTCTGAGGG ATTAACGGAT CTAATTGAGA TGCAGCTACT GGCCATGAAC 
CCGTTCCTTA TCTCCATGAA GCACAAGGCC CTGCAGGATC TGGACGACAC CGCCTCGGGG 
AATGGACGCA TCGTGGTCAG TCGCAAGCAA GTCGCTTGTC ATGAATATCC GCTGGCGGAC 
AAAGCTAGCA AAATGCCCCT CGATAACCTT ATTTTTAGTT CCGGATTATG GGAATTTATT 
CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262405.1
CDS	117..2702
Translation	MGNQLVGIAPSQIYAVEHYFSGQFGSEIAFSSNMGSTRFFKVAKAKTDEGQIVVKVFVKHDPTLPLEDHKERLEGIKKTLSLANAVNCLPFQRVELIDKAAYIMREYVKHSLYDRVSTRPFLTVLEKKWITFQILCALNQCHKQKICHGDIKLENILITSWNWILLSDFASFKPTYLPEDNPADYTYFFDTSRRRTCYIAPERFVKTLASDDDGGNGNMSVIHTDSIIRLAPSYAGNTLLPAMDIFSAGCALLELWTEGTAPFELSQLLAYRRGERDLVEKHLAGIENERLRNLLASMIDIHSMNRKSAEDYLDQERGQLFPEYFYSFLQSYLQMFSSTPIMSPDDKIQCLHKDIGHCIKVLTQAQDLTPEEEEKEEKKDPTLCARLPPEHDGLILIITVVTSCIRGLKQSNTKIAALELLQKLSKYTTSETILDRILPYILHLAQKSPAKVQVQAIETLTACLGMVKDIPRSDANVFPEYILPSITDLASETSAVIVRVAFARNIAALAKSAVYFLEETQRNAPNDMPTPRYEAELNALHDIVRQAVLSLLTDSQPVVKQTLMESGICDLCAFFGKEKANDVILSHIMTFLNDEDKNLRGAFYDNIAGVAGYVGWQASDILVPLLQQGFTDREEFVIAKAIRAVTILIELGHIKKPAVTEIVQETACYLCHPNLWVRHEICGMIATTARNLSAIDVQCKIMPAIGAFLKAPLIQVEKPHILLDCVHPPVPRQIFDSVLRFQDIHHFIRALEARSRVRSQTRQGALPQYEEMGQTLRNLFRRLSSEGLTDLIEMQLLAMNPFLISMKHKALQDLDDTASGNGRIVVSRKQVACHEYPLADKASKMPLDNLIFSSGLWEFIR

Target ORF information:

RefSeq Version	NM_001275476.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar protein sorting 15, transcript variant C (Vps15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275476.1

ORF Insert Sequence:

ATGGGCAATC AACTGGTGGG CATTGCCCCT TCGCAAATTT ATGCGGTGGA ACACTATTTT 
TCCGGCCAGT TTGGATCCGA GATCGCCTTC AGTTCCAATA TGGGCAGCAC GAGATTCTTC 
AAGGTGGCCA AGGCTAAAAC GGATGAGGGG CAAATAGTGG TCAAGGTGTT TGTGAAGCAC 
GATCCCACTT TGCCACTGGA GGATCACAAG GAGCGATTGG AGGGCATCAA GAAGACACTC 
TCGCTGGCCA ATGCCGTCAA TTGTTTACCC TTTCAGCGGG TCGAGCTTAT CGACAAGGCA 
GCCTACATAA TGCGGGAGTA TGTGAAGCAC AGTCTCTATG ATCGCGTATC CACACGACCA 
TTTCTCACCG TATTGGAGAA AAAGTGGATA ACGTTCCAAA TTTTGTGTGC CCTGAATCAG 
TGCCACAAAC AGAAGATCTG CCACGGGGAC ATCAAGCTGG AGAACATCTT GATTACGTCT 
TGGAACTGGA TTCTGCTCTC CGACTTTGCC TCCTTTAAGC CCACATATTT GCCGGAGGAC 
AATCCGGCGG ATTATACGTA CTTCTTCGAT ACGAGTAGAA GGCGAACCTG CTATATTGCA 
CCCGAACGAT TCGTTAAAAC ACTGGCCTCC GATGATGATG GTGGCAATGG TAATATGTCG 
GTCATACACA CCGATTCCAT AATTCGACTT GCTCCTAGCT ATGCGGGTAA CACTTTGTTG 
CCAGCCATGG ACATCTTTTC GGCAGGCTGT GCCTTGCTGG AACTCTGGAC AGAGGGAACT 
GCGCCCTTTG AACTCTCCCA GCTGCTGGCT TACAGGCGTG GCGAGCGAGA TCTGGTGGAG 
AAACATCTGG CGGGCATCGA AAACGAAAGG TTGCGCAATC TGCTGGCCTC TATGATTGAC 
ATACACTCGA TGAACCGAAA GAGCGCCGAG GATTACTTGG ACCAGGAGCG CGGTCAGTTG 
TTTCCCGAAT ACTTCTACTC CTTCCTGCAG TCGTACTTGC AAATGTTCAG CTCAACACCG 
ATCATGTCGC CGGATGACAA GATTCAGTGT CTGCATAAGG ACATTGGGCA CTGTATTAAA 
GTGCTGACAC AGGCCCAGGA TTTAACACCG GAAGAGGAGG AAAAGGAAGA AAAGAAGGAT 
CCCACGTTGT GTGCCCGACT GCCACCGGAG CATGATGGTC TCATTTTGAT CATCACGGTA 
GTCACATCCT GCATACGAGG ACTGAAGCAG TCGAATACCA AAATTGCAGC CTTGGAACTT 
CTGCAAAAGC TGTCGAAGTA CACAACATCC GAGACCATCC TGGATCGCAT TTTGCCCTAC 
ATTCTCCATT TGGCCCAGAA ATCGCCAGCC AAGGTGCAGG TTCAGGCCAT CGAAACTCTG 
ACCGCCTGTT TGGGCATGGT AAAGGACATT CCACGCAGCG ATGCCAACGT GTTTCCCGAG 
TACATTCTGC CCTCCATCAC CGATTTGGCC AGCGAGACGA GTGCAGTTAT TGTTCGCGTG 
GCCTTTGCCC GGAATATAGC TGCACTGGCC AAGAGCGCTG TCTACTTTTT GGAGGAAACG 
CAACGAAATG CTCCTAACGA TATGCCCACT CCACGTTACG AGGCAGAACT GAACGCCCTC 
CATGACATAG TTAGACAGGC AGTACTCAGT CTGTTAACGG ATTCCCAACC GGTGGTCAAG 
CAAACACTGA TGGAGTCGGG CATCTGCGAT CTCTGTGCCT TCTTTGGCAA GGAAAAAGCG 
AACGATGTGA TACTCTCGCA CATCATGACG TTCCTTAATG ATGAGGACAA GAACTTGAGA 
GGAGCATTCT ATGACAACAT CGCCGGAGTG GCTGGCTATG TGGGCTGGCA GGCGTCGGAC 
ATTCTCGTGC CACTTCTGCA GCAAGGTTTC ACGGATCGCG AGGAGTTCGT CATTGCAAAA 
GCCATTCGTG CTGTGACCAT TCTTATCGAA CTGGGTCACA TTAAGAAGCC AGCCGTTACG 
GAAATTGTCC AAGAGACAGC CTGTTATCTG TGCCATCCAA ATCTTTGGGT TCGTCACGAA 
ATCTGCGGCA TGATTGCCAC CACGGCGCGT AATCTCAGCG CCATCGATGT GCAGTGCAAG 
ATTATGCCGG CGATTGGGGC ATTTCTCAAG GCCCCGCTCA TCCAGGTGGA GAAACCGCAC 
ATCCTGTTGG ACTGTGTGCA TCCGCCTGTG CCGCGGCAGA TCTTCGACAG CGTTCTGCGC 
TTCCAAGACA TTCATCATTT TATCAGAGCA CTGGAGGCAC GATCACGTGT TCGTAGTCAG 
ACGAGGCAGG GAGCTCTGCC GCAGTACGAG GAGATGGGAC AGACCTTGCG TAATCTCTTT 
AGACGCTTAA GCTCTGAGGG ATTAACGGAT CTAATTGAGA TGCAGCTACT GGCCATGAAC 
CCGTTCCTTA TCTCCATGAA GCACAAGGCC CTGCAGGATC TGGACGACAC CGCCTCGGGG 
AATGGACGCA TCGTGGTCAG TCGCAAGCAA GTCGCTTGTC ATGAATATCC GCTGGCGGAC 
AAAGCTAGCA AAATGCCCCT CGATAACCTT ATTTTTAGTT CCGGATTATG GGAATTTATT 
CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vps15 ( NM_001170094.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163565.1 Drosophila melanogaster vacuolar protein sorting 15, transcript variant B (Vps15), mRNA.

Price & Availability↑

CloneID	OFa04321
Clone ID Related Accession (Same CDS sequence)	NM_001170094.2
Accession Version	NM_001170094.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar protein sorting 15, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170094.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAATC AACTGGTGGG CATTGCCCCT TCGCAAATTT ATGCGGTGGA ACACTATTTT 
TCCGGCCAGT TTGGATCCGA GATCGCCTTC AGTTCCAATA TGGGCAGCAC GAGATTCTTC 
AAGGTGGCCA AGGCTAAAAC GGATGAGGGG CAAATAGTGG TCAAGGTGTT TGTGAAGCAC 
GATCCCACTT TGCCACTGGA GGATCACAAG GAGCGATTGG AGGGCATCAA GAAGACACTC 
TCGCTGGCCA ATGCCGTCAA TTGTTTACCC TTTCAGCGGG TCGAGCTTAT CGACAAGGCA 
GCCTACATAA TGCGGGAGTA TGTGAAGCAC AGTCTCTATG ATCGCGTATC CACACGACCA 
TTTCTCACCG TATTGGAGAA AAAGTGGATA ACGTTCCAAA TTTTGTGTGC CCTGAATCAG 
TGCCACAAAC AGAAGATCTG CCACGGGGAC ATCAAGCTGG AGAACATCTT GATTACGTCT 
TGGAACTGGA TTCTGCTCTC CGACTTTGCC TCCTTTAAGC CCACATATTT GCCGGAGGAC 
AATCCGGCGG ATTATACGTA CTTCTTCGAT ACGAGTAGAA GGCGAACCTG CTATATTGCA 
CCCGAACGAT TCGTTAAAAC ACTGGCCTCC GATGATGATG GTGGCAATGG TAATATGTCG 
GTCATACACA CCGATTCCAT AATTCGACTT GCTCCTAGCT ATGCGGGTAA CACTTTGTTG 
CCAGCCATGG ACATCTTTTC GGCAGGCTGT GCCTTGCTGG AACTCTGGAC AGAGGGAACT 
GCGCCCTTTG AACTCTCCCA GCTGCTGGCT TACAGGCGTG GCGAGCGAGA TCTGGTGGAG 
AAACATCTGG CGGGCATCGA AAACGAAAGG TTGCGCAATC TGCTGGCCTC TATGATTGAC 
ATACACTCGA TGAACCGAAA GAGCGCCGAG GATTACTTGG ACCAGGAGCG CGGTCAGTTG 
TTTCCCGAAT ACTTCTACTC CTTCCTGCAG TCGTACTTGC AAATGTTCAG CTCAACACCG 
ATCATGTCGC CGGATGACAA GATTCAGTGT CTGCATAAGG ACATTGGGCA CTGTATTAAA 
GTGCTGACAC AGGCCCAGGA TTTAACACCG GAAGAGGAGG AAAAGGAAGA AAAGAAGGAT 
CCCACGTTGT GTGCCCGACT GCCACCGGAG CATGATGGTC TCATTTTGAT CATCACGGTA 
GTCACATCCT GCATACGAGG ACTGAAGCAG TCGAATACCA AAATTGCAGC CTTGGAACTT 
CTGCAAAAGC TGTCGAAGTA CACAACATCC GAGACCATCC TGGATCGCAT TTTGCCCTAC 
ATTCTCCATT TGGCCCAGAA ATCGCCAGCC AAGGTGCAGG TTCAGGCCAT CGAAACTCTG 
ACCGCCTGTT TGGGCATGGT AAAGGACATT CCACGCAGCG ATGCCAACGT GTTTCCCGAG 
TACATTCTGC CCTCCATCAC CGATTTGGCC AGCGAGACGA GTGCAGTTAT TGTTCGCGTG 
GCCTTTGCCC GGAATATAGC TGCACTGGCC AAGAGCGCTG TCTACTTTTT GGAGGAAACG 
CAACGAAATG CTCCTAACGA TATGCCCACT CCACGTTACG AGGCAGAACT GAACGCCCTC 
CATGACATAG TTAGACAGGC AGTACTCAGT CTGTTAACGG ATTCCCAACC GGTGGTCAAG 
CAAACACTGA TGGAGTCGGG CATCTGCGAT CTCTGTGCCT TCTTTGGCAA GGAAAAAGCG 
AACGATGTGA TACTCTCGCA CATCATGACG TTCCTTAATG ATGAGGACAA GAACTTGAGA 
GGAGCATTCT ATGACAACAT CGCCGGAGTG GCTGGCTATG TGGGCTGGCA GGCGTCGGAC 
ATTCTCGTGC CACTTCTGCA GCAAGGTTTC ACGGATCGCG AGGAGTTCGT CATTGCAAAA 
GCCATTCGTG CTGTGACCAT TCTTATCGAA CTGGGTCACA TTAAGAAGCC AGCCGTTACG 
GAAATTGTCC AAGAGACAGC CTGTTATCTG TGCCATCCAA ATCTTTGGGT TCGTCACGAA 
ATCTGCGGCA TGATTGCCAC CACGGCGCGT AATCTCAGCG CCATCGATGT GCAGTGCAAG 
ATTATGCCGG CGATTGGGGC ATTTCTCAAG GCCCCGCTCA TCCAGGTGGA GAAACCGCAC 
ATCCTGTTGG ACTGTGTGCA TCCGCCTGTG CCGCGGCAGA TCTTCGACAG CGTTCTGCGC 
TTCCAAGACA TTCATCATTT TATCAGAGCA CTGGAGGCAC GATCACGTGT TCGTAGTCAG 
ACGAGGCAGG GAGCTCTGCC GCAGTACGAG GAGATGGGAC AGACCTTGCG TAATCTCTTT 
AGACGCTTAA GCTCTGAGGG ATTAACGGAT CTAATTGAGA TGCAGCTACT GGCCATGAAC 
CCGTTCCTTA TCTCCATGAA GCACAAGGCC CTGCAGGATC TGGACGACAC CGCCTCGGGG 
AATGGACGCA TCGTGGTCAG TCGCAAGCAA GTCGCTTGTC ATGAATATCC GCTGGCGGAC 
AAAGCTAGCA AAATGCCCCT CGATAACCGT AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163565.1
CDS	117..2672
Translation	MGNQLVGIAPSQIYAVEHYFSGQFGSEIAFSSNMGSTRFFKVAKAKTDEGQIVVKVFVKHDPTLPLEDHKERLEGIKKTLSLANAVNCLPFQRVELIDKAAYIMREYVKHSLYDRVSTRPFLTVLEKKWITFQILCALNQCHKQKICHGDIKLENILITSWNWILLSDFASFKPTYLPEDNPADYTYFFDTSRRRTCYIAPERFVKTLASDDDGGNGNMSVIHTDSIIRLAPSYAGNTLLPAMDIFSAGCALLELWTEGTAPFELSQLLAYRRGERDLVEKHLAGIENERLRNLLASMIDIHSMNRKSAEDYLDQERGQLFPEYFYSFLQSYLQMFSSTPIMSPDDKIQCLHKDIGHCIKVLTQAQDLTPEEEEKEEKKDPTLCARLPPEHDGLILIITVVTSCIRGLKQSNTKIAALELLQKLSKYTTSETILDRILPYILHLAQKSPAKVQVQAIETLTACLGMVKDIPRSDANVFPEYILPSITDLASETSAVIVRVAFARNIAALAKSAVYFLEETQRNAPNDMPTPRYEAELNALHDIVRQAVLSLLTDSQPVVKQTLMESGICDLCAFFGKEKANDVILSHIMTFLNDEDKNLRGAFYDNIAGVAGYVGWQASDILVPLLQQGFTDREEFVIAKAIRAVTILIELGHIKKPAVTEIVQETACYLCHPNLWVRHEICGMIATTARNLSAIDVQCKIMPAIGAFLKAPLIQVEKPHILLDCVHPPVPRQIFDSVLRFQDIHHFIRALEARSRVRSQTRQGALPQYEEMGQTLRNLFRRLSSEGLTDLIEMQLLAMNPFLISMKHKALQDLDDTASGNGRIVVSRKQVACHEYPLADKASKMPLDNRK

Target ORF information:

RefSeq Version	NM_001170094.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar protein sorting 15, transcript variant B (Vps15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170094.2

ORF Insert Sequence:

ATGGGCAATC AACTGGTGGG CATTGCCCCT TCGCAAATTT ATGCGGTGGA ACACTATTTT 
TCCGGCCAGT TTGGATCCGA GATCGCCTTC AGTTCCAATA TGGGCAGCAC GAGATTCTTC 
AAGGTGGCCA AGGCTAAAAC GGATGAGGGG CAAATAGTGG TCAAGGTGTT TGTGAAGCAC 
GATCCCACTT TGCCACTGGA GGATCACAAG GAGCGATTGG AGGGCATCAA GAAGACACTC 
TCGCTGGCCA ATGCCGTCAA TTGTTTACCC TTTCAGCGGG TCGAGCTTAT CGACAAGGCA 
GCCTACATAA TGCGGGAGTA TGTGAAGCAC AGTCTCTATG ATCGCGTATC CACACGACCA 
TTTCTCACCG TATTGGAGAA AAAGTGGATA ACGTTCCAAA TTTTGTGTGC CCTGAATCAG 
TGCCACAAAC AGAAGATCTG CCACGGGGAC ATCAAGCTGG AGAACATCTT GATTACGTCT 
TGGAACTGGA TTCTGCTCTC CGACTTTGCC TCCTTTAAGC CCACATATTT GCCGGAGGAC 
AATCCGGCGG ATTATACGTA CTTCTTCGAT ACGAGTAGAA GGCGAACCTG CTATATTGCA 
CCCGAACGAT TCGTTAAAAC ACTGGCCTCC GATGATGATG GTGGCAATGG TAATATGTCG 
GTCATACACA CCGATTCCAT AATTCGACTT GCTCCTAGCT ATGCGGGTAA CACTTTGTTG 
CCAGCCATGG ACATCTTTTC GGCAGGCTGT GCCTTGCTGG AACTCTGGAC AGAGGGAACT 
GCGCCCTTTG AACTCTCCCA GCTGCTGGCT TACAGGCGTG GCGAGCGAGA TCTGGTGGAG 
AAACATCTGG CGGGCATCGA AAACGAAAGG TTGCGCAATC TGCTGGCCTC TATGATTGAC 
ATACACTCGA TGAACCGAAA GAGCGCCGAG GATTACTTGG ACCAGGAGCG CGGTCAGTTG 
TTTCCCGAAT ACTTCTACTC CTTCCTGCAG TCGTACTTGC AAATGTTCAG CTCAACACCG 
ATCATGTCGC CGGATGACAA GATTCAGTGT CTGCATAAGG ACATTGGGCA CTGTATTAAA 
GTGCTGACAC AGGCCCAGGA TTTAACACCG GAAGAGGAGG AAAAGGAAGA AAAGAAGGAT 
CCCACGTTGT GTGCCCGACT GCCACCGGAG CATGATGGTC TCATTTTGAT CATCACGGTA 
GTCACATCCT GCATACGAGG ACTGAAGCAG TCGAATACCA AAATTGCAGC CTTGGAACTT 
CTGCAAAAGC TGTCGAAGTA CACAACATCC GAGACCATCC TGGATCGCAT TTTGCCCTAC 
ATTCTCCATT TGGCCCAGAA ATCGCCAGCC AAGGTGCAGG TTCAGGCCAT CGAAACTCTG 
ACCGCCTGTT TGGGCATGGT AAAGGACATT CCACGCAGCG ATGCCAACGT GTTTCCCGAG 
TACATTCTGC CCTCCATCAC CGATTTGGCC AGCGAGACGA GTGCAGTTAT TGTTCGCGTG 
GCCTTTGCCC GGAATATAGC TGCACTGGCC AAGAGCGCTG TCTACTTTTT GGAGGAAACG 
CAACGAAATG CTCCTAACGA TATGCCCACT CCACGTTACG AGGCAGAACT GAACGCCCTC 
CATGACATAG TTAGACAGGC AGTACTCAGT CTGTTAACGG ATTCCCAACC GGTGGTCAAG 
CAAACACTGA TGGAGTCGGG CATCTGCGAT CTCTGTGCCT TCTTTGGCAA GGAAAAAGCG 
AACGATGTGA TACTCTCGCA CATCATGACG TTCCTTAATG ATGAGGACAA GAACTTGAGA 
GGAGCATTCT ATGACAACAT CGCCGGAGTG GCTGGCTATG TGGGCTGGCA GGCGTCGGAC 
ATTCTCGTGC CACTTCTGCA GCAAGGTTTC ACGGATCGCG AGGAGTTCGT CATTGCAAAA 
GCCATTCGTG CTGTGACCAT TCTTATCGAA CTGGGTCACA TTAAGAAGCC AGCCGTTACG 
GAAATTGTCC AAGAGACAGC CTGTTATCTG TGCCATCCAA ATCTTTGGGT TCGTCACGAA 
ATCTGCGGCA TGATTGCCAC CACGGCGCGT AATCTCAGCG CCATCGATGT GCAGTGCAAG 
ATTATGCCGG CGATTGGGGC ATTTCTCAAG GCCCCGCTCA TCCAGGTGGA GAAACCGCAC 
ATCCTGTTGG ACTGTGTGCA TCCGCCTGTG CCGCGGCAGA TCTTCGACAG CGTTCTGCGC 
TTCCAAGACA TTCATCATTT TATCAGAGCA CTGGAGGCAC GATCACGTGT TCGTAGTCAG 
ACGAGGCAGG GAGCTCTGCC GCAGTACGAG GAGATGGGAC AGACCTTGCG TAATCTCTTT 
AGACGCTTAA GCTCTGAGGG ATTAACGGAT CTAATTGAGA TGCAGCTACT GGCCATGAAC 
CCGTTCCTTA TCTCCATGAA GCACAAGGCC CTGCAGGATC TGGACGACAC CGCCTCGGGG 
AATGGACGCA TCGTGGTCAG TCGCAAGCAA GTCGCTTGTC ATGAATATCC GCTGGCGGAC 
AAAGCTAGCA AAATGCCCCT CGATAACCGT AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vps15 ( NM_141611.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649868.1 Drosophila melanogaster vacuolar protein sorting 15, transcript variant A (Vps15), mRNA.

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CloneID	OFa08174
Clone ID Related Accession (Same CDS sequence)	NM_141611.2
Accession Version	NM_141611.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar protein sorting 15, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141611.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAATC AACTGGTGGG CATTGCCCCT TCGCAAATTT ATGCGGTGGA ACACTATTTT 
TCCGGCCAGT TTGGATCCGA GATCGCCTTC AGTTCCAATA TGGGCAGCAC GAGATTCTTC 
AAGGTGGCCA AGGCTAAAAC GGATGAGGGG CAAATAGTGG TCAAGGTGTT TGTGAAGCAC 
GATCCCACTT TGCCACTGGA GGATCACAAG GAGCGATTGG AGGGCATCAA GAAGACACTC 
TCGCTGGCCA ATGCCGTCAA TTGTTTACCC TTTCAGCGGG TCGAGCTTAT CGACAAGGCA 
GCCTACATAA TGCGGGAGTA TGTGAAGCAC AGTCTCTATG ATCGCGTATC CACACGACCA 
TTTCTCACCG TATTGGAGAA AAAGTGGATA ACGTTCCAAA TTTTGTGTGC CCTGAATCAG 
TGCCACAAAC AGAAGATCTG CCACGGGGAC ATCAAGCTGG AGAACATCTT GATTACGTCT 
TGGAACTGGA TTCTGCTCTC CGACTTTGCC TCCTTTAAGC CCACATATTT GCCGGAGGAC 
AATCCGGCGG ATTATACGTA CTTCTTCGAT ACGAGTAGAA GGCGAACCTG CTATATTGCA 
CCCGAACGAT TCGTTAAAAC ACTGGCCTCC GATGATGATG GTGGCAATGG TAATATGTCG 
GTCATACACA CCGATTCCAT AATTCGACTT GCTCCTAGCT ATGCGGGTAA CACTTTGTTG 
CCAGCCATGG ACATCTTTTC GGCAGGCTGT GCCTTGCTGG AACTCTGGAC AGAGGGAACT 
GCGCCCTTTG AACTCTCCCA GCTGCTGGCT TACAGGCGTG GCGAGCGAGA TCTGGTGGAG 
AAACATCTGG CGGGCATCGA AAACGAAAGG TTGCGCAATC TGCTGGCCTC TATGATTGAC 
ATACACTCGA TGAACCGAAA GAGCGCCGAG GATTACTTGG ACCAGGAGCG CGGTCAGTTG 
TTTCCCGAAT ACTTCTACTC CTTCCTGCAG TCGTACTTGC AAATGTTCAG CTCAACACCG 
ATCATGTCGC CGGATGACAA GATTCAGTGT CTGCATAAGG ACATTGGGCA CTGTATTAAA 
GTGCTGACAC AGGCCCAGGA TTTAACACCG GAAGAGGAGG AAAAGGAAGA AAAGAAGGAT 
CCCACGTTGT GTGCCCGACT GCCACCGGAG CATGATGGTC TCATTTTGAT CATCACGGTA 
GTCACATCCT GCATACGAGG ACTGAAGCAG TCGAATACCA AAATTGCAGC CTTGGAACTT 
CTGCAAAAGC TGTCGAAGTA CACAACATCC GAGACCATCC TGGATCGCAT TTTGCCCTAC 
ATTCTCCATT TGGCCCAGAA ATCGCCAGCC AAGGTGCAGG TTCAGGCCAT CGAAACTCTG 
ACCGCCTGTT TGGGCATGGT AAAGGACATT CCACGCAGCG ATGCCAACGT GTTTCCCGAG 
TACATTCTGC CCTCCATCAC CGATTTGGCC AGCGAGACGA GTGCAGTTAT TGTTCGCGTG 
GCCTTTGCCC GGAATATAGC TGCACTGGCC AAGAGCGCTG TCTACTTTTT GGAGGAAACG 
CAACGAAATG CTCCTAACGA TATGCCCACT CCACGTTACG AGGCAGAACT GAACGCCCTC 
CATGACATAG TTAGACAGGC AGTACTCAGT CTGTTAACGG ATTCCCAACC GGTGGTCAAG 
CAAACACTGA TGGAGTCGGG CATCTGCGAT CTCTGTGCCT TCTTTGGCAA GGAAAAAGCG 
AACGATGTGA TACTCTCGCA CATCATGACG TTCCTTAATG ATGAGGACAA GAACTTGAGA 
GGAGCATTCT ATGACAACAT CGCCGGAGTG GCTGGCTATG TGGGCTGGCA GGCGTCGGAC 
ATTCTCGTGC CACTTCTGCA GCAAGGTTTC ACGGATCGCG AGGAGTTCGT CATTGCAAAA 
GCCATTCGTG CTGTGACCAT TCTTATCGAA CTGGGTCACA TTAAGAAGCC AGCCGTTACG 
GAAATTGTCC AAGAGACAGC CTGTTATCTG TGCCATCCAA ATCTTTGGGT TCGTCACGAA 
ATCTGCGGCA TGATTGCCAC CACGGCGCGT AATCTCAGCG CCATCGATGT GCAGTGCAAG 
ATTATGCCGG CGATTGGGGC ATTTCTCAAG GCCCCGCTCA TCCAGGTGGA GAAACCGCAC 
ATCCTGTTGG ACTGTGTGCA TCCGCCTGTG CCGCGGCAGA TCTTCGACAG CGTTCTGCGC 
TTCCAAGACA TTCATCATTT TATCAGAGCA CTGGAGGCAC GATCACGTGT TCGTAGTCAG 
ACGAGGCAGG GAGCTCTGCC GCAGTACGAG GAGATGGGAC AGACCTTGCG TAATCTCTTT 
AGACGCTTAA GCTCTGAGGG ATTAACGGAT CTAATTGAGA TGCAGCTACT GGCCATGAAC 
CCGTTCCTTA TCTCCATGAA GCACAAGGCC CTGCAGGATC TGGACGACAC CGCCTCGGGG 
AATGGACGCA TCGTGGTCAG TCGCAAGCAA GTCGCTTGTC ATGAATATCC GCTGGCGGAC 
AAAGCTAGCA AAATGCCCCT CGATAACCGC TCCAGCGAGG GTCCAACTCT AGCTTCGCCC 
GCCTCGGATG CAGCAATCAC ACCGCTGGGA GCTGGCGGAC TGGCCGGAAG TCCGGCTTCT 
GGAGCCGTAG TCCTTGGAGT GCCAACTGCC ACAGTGAGTG CGGCCACTTC GCTGGGTGAA 
TACACAATGC CGGAACGAAA CTGTTGGCTG GAACGCTCCT CCGAGTGCCG CAAGGATCTC 
GAATCGCTGA CGGCCAAGAT TAAGAGTCGC TACAATGTGT TGGCCATCTC GCAGCGTTGC 
AGCTACGACA AGCTGGTGAC ACCCTATCCG CCAGGCTGGC GGATGACTGG CACTTTGGTG 
GCTCACCTGC ACGAGCATTC GGAATCCGTG ATCAAGCTGG CTTCATTGCG GCCCCACGGA 
TCACTCTTCG CCAGCGGCAG CATAGACGGC ACAGTGCGAT TGTGGGATTG CAGCAAACTG 
AATGGCAATC AGGGTGTAAA CAAATCGAGG CAGGTTTATT CGGCCAATAC ACCCATCTAT 
GCACTGGCGG CCTGTGATAG TGGACAATCG CTGGCGGTGG GCGGCAAGGA TGGCAGCATG 
CTGATGATGC GAATCGATCG GAATTCCGCC AAAATGACGC TTCAACAGGC CCTAAATCAA 
AATGATCATA AAGACGGTCC TGTTGTGGAC ATGCACGCCT ACGATCAGGC CACGCAAAGT 
GTCATCGTGT ACGCCACGCT ATATGGCGGC ATTGTGGCCT GGGACACGAG GATGCAGCAC 
AGCGCCTGGA GGCTACAGAA TGAACTCCGT CATGGGGTGA TCACAACGAT CTGTGCGGAT 
CCAACGGGAT CTTGGCTGGC CACCGGTACC AGCGGCGGTA AGCACATCTG CTGGGATCTA 
CGCTTCCGTC TGCCCATTGC GGAAATCAAG CATCCGGCGG ATTCGTGGAT ACGCAAGGTG 
GCCTGCCATC CCACAGAGCC TTCATACCTG ATCTCAGCCT CGCAGTCAAA CAATGAGGTA 
TCCGTGTGGA ATATAGAAAC GGGACAGCGC CAGACGGTTT TGTGGGCAAG TCCGGTGGCG 
GCTTTATCGA GCGCTAGTCC CAGCGATCCG GCCACCACGT GCGGCATTCT CAGTGGCGTG 
GTCGATGGTT CACCCTTTAT TCTCACCGGC AGTTCGGATC AGCGGATCCG GTACTGGGAC 
ATCACCAACC CAAAGAACTC ATCGCTTAAA GTACCCGCTG CCAATGACAA CCTGGCAGAT 
GTGGCTTTCA ACTATGGTGC CCGGTTAATC GATGGCAGCC AAGTGATCGA GGAGCAAATC 
ATTTCCATGG CCAGCACGGG CGACTCGCAG CAGCTGCGAT CGTCCACGGA GGAGAATCCC 
CGCTCGGGGC CGGATATGCC AACGGCCAGT CACCACGATG CCATCACGGA TCTGCTGATG 
TGCAAGGACA AGGGCCAGAT CTACATAGCA TCTGCATCGC GAAACGGTGT CATCAAGCTG 
TGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649868.1
CDS	117..4145
Translation	MGNQLVGIAPSQIYAVEHYFSGQFGSEIAFSSNMGSTRFFKVAKAKTDEGQIVVKVFVKHDPTLPLEDHKERLEGIKKTLSLANAVNCLPFQRVELIDKAAYIMREYVKHSLYDRVSTRPFLTVLEKKWITFQILCALNQCHKQKICHGDIKLENILITSWNWILLSDFASFKPTYLPEDNPADYTYFFDTSRRRTCYIAPERFVKTLASDDDGGNGNMSVIHTDSIIRLAPSYAGNTLLPAMDIFSAGCALLELWTEGTAPFELSQLLAYRRGERDLVEKHLAGIENERLRNLLASMIDIHSMNRKSAEDYLDQERGQLFPEYFYSFLQSYLQMFSSTPIMSPDDKIQCLHKDIGHCIKVLTQAQDLTPEEEEKEEKKDPTLCARLPPEHDGLILIITVVTSCIRGLKQSNTKIAALELLQKLSKYTTSETILDRILPYILHLAQKSPAKVQVQAIETLTACLGMVKDIPRSDANVFPEYILPSITDLASETSAVIVRVAFARNIAALAKSAVYFLEETQRNAPNDMPTPRYEAELNALHDIVRQAVLSLLTDSQPVVKQTLMESGICDLCAFFGKEKANDVILSHIMTFLNDEDKNLRGAFYDNIAGVAGYVGWQASDILVPLLQQGFTDREEFVIAKAIRAVTILIELGHIKKPAVTEIVQETACYLCHPNLWVRHEICGMIATTARNLSAIDVQCKIMPAIGAFLKAPLIQVEKPHILLDCVHPPVPRQIFDSVLRFQDIHHFIRALEARSRVRSQTRQGALPQYEEMGQTLRNLFRRLSSEGLTDLIEMQLLAMNPFLISMKHKALQDLDDTASGNGRIVVSRKQVACHEYPLADKASKMPLDNRSSEGPTLASPASDAAITPLGAGGLAGSPASGAVVLGVPTATVSAATSLGEYTMPERNCWLERSSECRKDLESLTAKIKSRYNVLAISQRCSYDKLVTPYPPGWRMTGTLVAHLHEHSESVIKLASLRPHGSLFASGSIDGTVRLWDCSKLNGNQGVNKSRQVYSANTPIYALAACDSGQSLAVGGKDGSMLMMRIDRNSAKMTLQQALNQNDHKDGPVVDMHAYDQATQSVIVYATLYGGIVAWDTRMQHSAWRLQNELRHGVITTICADPTGSWLATGTSGGKHICWDLRFRLPIAEIKHPADSWIRKVACHPTEPSYLISASQSNNEVSVWNIETGQRQTVLWASPVAALSSASPSDPATTCGILSGVVDGSPFILTGSSDQRIRYWDITNPKNSSLKVPAANDNLADVAFNYGARLIDGSQVIEEQIISMASTGDSQQLRSSTEENPRSGPDMPTASHHDAITDLLMCKDKGQIYIASASRNGVIKLWK

Target ORF information:

RefSeq Version	NM_141611.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar protein sorting 15, transcript variant A (Vps15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141611.2

ORF Insert Sequence:

ATGGGCAATC AACTGGTGGG CATTGCCCCT TCGCAAATTT ATGCGGTGGA ACACTATTTT 
TCCGGCCAGT TTGGATCCGA GATCGCCTTC AGTTCCAATA TGGGCAGCAC GAGATTCTTC 
AAGGTGGCCA AGGCTAAAAC GGATGAGGGG CAAATAGTGG TCAAGGTGTT TGTGAAGCAC 
GATCCCACTT TGCCACTGGA GGATCACAAG GAGCGATTGG AGGGCATCAA GAAGACACTC 
TCGCTGGCCA ATGCCGTCAA TTGTTTACCC TTTCAGCGGG TCGAGCTTAT CGACAAGGCA 
GCCTACATAA TGCGGGAGTA TGTGAAGCAC AGTCTCTATG ATCGCGTATC CACACGACCA 
TTTCTCACCG TATTGGAGAA AAAGTGGATA ACGTTCCAAA TTTTGTGTGC CCTGAATCAG 
TGCCACAAAC AGAAGATCTG CCACGGGGAC ATCAAGCTGG AGAACATCTT GATTACGTCT 
TGGAACTGGA TTCTGCTCTC CGACTTTGCC TCCTTTAAGC CCACATATTT GCCGGAGGAC 
AATCCGGCGG ATTATACGTA CTTCTTCGAT ACGAGTAGAA GGCGAACCTG CTATATTGCA 
CCCGAACGAT TCGTTAAAAC ACTGGCCTCC GATGATGATG GTGGCAATGG TAATATGTCG 
GTCATACACA CCGATTCCAT AATTCGACTT GCTCCTAGCT ATGCGGGTAA CACTTTGTTG 
CCAGCCATGG ACATCTTTTC GGCAGGCTGT GCCTTGCTGG AACTCTGGAC AGAGGGAACT 
GCGCCCTTTG AACTCTCCCA GCTGCTGGCT TACAGGCGTG GCGAGCGAGA TCTGGTGGAG 
AAACATCTGG CGGGCATCGA AAACGAAAGG TTGCGCAATC TGCTGGCCTC TATGATTGAC 
ATACACTCGA TGAACCGAAA GAGCGCCGAG GATTACTTGG ACCAGGAGCG CGGTCAGTTG 
TTTCCCGAAT ACTTCTACTC CTTCCTGCAG TCGTACTTGC AAATGTTCAG CTCAACACCG 
ATCATGTCGC CGGATGACAA GATTCAGTGT CTGCATAAGG ACATTGGGCA CTGTATTAAA 
GTGCTGACAC AGGCCCAGGA TTTAACACCG GAAGAGGAGG AAAAGGAAGA AAAGAAGGAT 
CCCACGTTGT GTGCCCGACT GCCACCGGAG CATGATGGTC TCATTTTGAT CATCACGGTA 
GTCACATCCT GCATACGAGG ACTGAAGCAG TCGAATACCA AAATTGCAGC CTTGGAACTT 
CTGCAAAAGC TGTCGAAGTA CACAACATCC GAGACCATCC TGGATCGCAT TTTGCCCTAC 
ATTCTCCATT TGGCCCAGAA ATCGCCAGCC AAGGTGCAGG TTCAGGCCAT CGAAACTCTG 
ACCGCCTGTT TGGGCATGGT AAAGGACATT CCACGCAGCG ATGCCAACGT GTTTCCCGAG 
TACATTCTGC CCTCCATCAC CGATTTGGCC AGCGAGACGA GTGCAGTTAT TGTTCGCGTG 
GCCTTTGCCC GGAATATAGC TGCACTGGCC AAGAGCGCTG TCTACTTTTT GGAGGAAACG 
CAACGAAATG CTCCTAACGA TATGCCCACT CCACGTTACG AGGCAGAACT GAACGCCCTC 
CATGACATAG TTAGACAGGC AGTACTCAGT CTGTTAACGG ATTCCCAACC GGTGGTCAAG 
CAAACACTGA TGGAGTCGGG CATCTGCGAT CTCTGTGCCT TCTTTGGCAA GGAAAAAGCG 
AACGATGTGA TACTCTCGCA CATCATGACG TTCCTTAATG ATGAGGACAA GAACTTGAGA 
GGAGCATTCT ATGACAACAT CGCCGGAGTG GCTGGCTATG TGGGCTGGCA GGCGTCGGAC 
ATTCTCGTGC CACTTCTGCA GCAAGGTTTC ACGGATCGCG AGGAGTTCGT CATTGCAAAA 
GCCATTCGTG CTGTGACCAT TCTTATCGAA CTGGGTCACA TTAAGAAGCC AGCCGTTACG 
GAAATTGTCC AAGAGACAGC CTGTTATCTG TGCCATCCAA ATCTTTGGGT TCGTCACGAA 
ATCTGCGGCA TGATTGCCAC CACGGCGCGT AATCTCAGCG CCATCGATGT GCAGTGCAAG 
ATTATGCCGG CGATTGGGGC ATTTCTCAAG GCCCCGCTCA TCCAGGTGGA GAAACCGCAC 
ATCCTGTTGG ACTGTGTGCA TCCGCCTGTG CCGCGGCAGA TCTTCGACAG CGTTCTGCGC 
TTCCAAGACA TTCATCATTT TATCAGAGCA CTGGAGGCAC GATCACGTGT TCGTAGTCAG 
ACGAGGCAGG GAGCTCTGCC GCAGTACGAG GAGATGGGAC AGACCTTGCG TAATCTCTTT 
AGACGCTTAA GCTCTGAGGG ATTAACGGAT CTAATTGAGA TGCAGCTACT GGCCATGAAC 
CCGTTCCTTA TCTCCATGAA GCACAAGGCC CTGCAGGATC TGGACGACAC CGCCTCGGGG 
AATGGACGCA TCGTGGTCAG TCGCAAGCAA GTCGCTTGTC ATGAATATCC GCTGGCGGAC 
AAAGCTAGCA AAATGCCCCT CGATAACCGC TCCAGCGAGG GTCCAACTCT AGCTTCGCCC 
GCCTCGGATG CAGCAATCAC ACCGCTGGGA GCTGGCGGAC TGGCCGGAAG TCCGGCTTCT 
GGAGCCGTAG TCCTTGGAGT GCCAACTGCC ACAGTGAGTG CGGCCACTTC GCTGGGTGAA 
TACACAATGC CGGAACGAAA CTGTTGGCTG GAACGCTCCT CCGAGTGCCG CAAGGATCTC 
GAATCGCTGA CGGCCAAGAT TAAGAGTCGC TACAATGTGT TGGCCATCTC GCAGCGTTGC 
AGCTACGACA AGCTGGTGAC ACCCTATCCG CCAGGCTGGC GGATGACTGG CACTTTGGTG 
GCTCACCTGC ACGAGCATTC GGAATCCGTG ATCAAGCTGG CTTCATTGCG GCCCCACGGA 
TCACTCTTCG CCAGCGGCAG CATAGACGGC ACAGTGCGAT TGTGGGATTG CAGCAAACTG 
AATGGCAATC AGGGTGTAAA CAAATCGAGG CAGGTTTATT CGGCCAATAC ACCCATCTAT 
GCACTGGCGG CCTGTGATAG TGGACAATCG CTGGCGGTGG GCGGCAAGGA TGGCAGCATG 
CTGATGATGC GAATCGATCG GAATTCCGCC AAAATGACGC TTCAACAGGC CCTAAATCAA 
AATGATCATA AAGACGGTCC TGTTGTGGAC ATGCACGCCT ACGATCAGGC CACGCAAAGT 
GTCATCGTGT ACGCCACGCT ATATGGCGGC ATTGTGGCCT GGGACACGAG GATGCAGCAC 
AGCGCCTGGA GGCTACAGAA TGAACTCCGT CATGGGGTGA TCACAACGAT CTGTGCGGAT 
CCAACGGGAT CTTGGCTGGC CACCGGTACC AGCGGCGGTA AGCACATCTG CTGGGATCTA 
CGCTTCCGTC TGCCCATTGC GGAAATCAAG CATCCGGCGG ATTCGTGGAT ACGCAAGGTG 
GCCTGCCATC CCACAGAGCC TTCATACCTG ATCTCAGCCT CGCAGTCAAA CAATGAGGTA 
TCCGTGTGGA ATATAGAAAC GGGACAGCGC CAGACGGTTT TGTGGGCAAG TCCGGTGGCG 
GCTTTATCGA GCGCTAGTCC CAGCGATCCG GCCACCACGT GCGGCATTCT CAGTGGCGTG 
GTCGATGGTT CACCCTTTAT TCTCACCGGC AGTTCGGATC AGCGGATCCG GTACTGGGAC 
ATCACCAACC CAAAGAACTC ATCGCTTAAA GTACCCGCTG CCAATGACAA CCTGGCAGAT 
GTGGCTTTCA ACTATGGTGC CCGGTTAATC GATGGCAGCC AAGTGATCGA GGAGCAAATC 
ATTTCCATGG CCAGCACGGG CGACTCGCAG CAGCTGCGAT CGTCCACGGA GGAGAATCCC 
CGCTCGGGGC CGGATATGCC AACGGCCAGT CACCACGATG CCATCACGGA TCTGCTGATG 
TGCAAGGACA AGGGCCAGAT CTACATAGCA TCTGCATCGC GAAACGGTGT CATCAAGCTG 
TGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Vps15 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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