rn cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	rn
Entrez Gene ID	40879
Full Name	rotund
Synonyms	5.3,CG10040,CG14600,CG14601,CG14603,CG32466,CG32467,CG42277,Dipr,Dmel\CG42277,Dmel_CG32466,Dmel_CG32467,P5.3,Rn,roe,tod,tot
General protein information	Preferred Names rotund Names CG42277 gene product from transcript CG42277-RE CG42277-PC CG42277-PE CG42277-PF CG42277-PG Distal-into-proximal T-one deficient rn-PC rn-PE rn-PF rn-PG roughened eye t-one transformed
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 84D3-84D3\|3-47.6 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001144549.2	NP_001138021.1	rotund, isoform F
NM_001275414.2	NP_001262343.1	rotund, isoform G
NM_206456.2	NP_996178.1	rotund, isoform C
NM_141458.3	NP_649715.2	rotund, isoform E

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we find that C15 interacts physically with the Dll activator through contacts between their homeodomain and binds competitively with Dll to adjacent cognate sites on LAE, adding potential new layers of regulation by C15. Lastly, we show that C15 and Bowl activities regulate also rn expression
Title: Tissue-specific enhancer repression through molecular integration of cell signaling inputs.

Joint ectopic expression of Dll and Rn is sufficient to cell-autonomously activate endogenous bab2 and LAE-driven reporter expression in wing and haltere cells
Title: Drosophila distal-less and Rotund bind a single enhancer ensuring reliable and robust bric-a-brac2 expression in distinct limb morphogenetic fields.

Roe acts as a transcriptional repressor in a negative-feedback loop of the N pathway.
Title: Self-modulation of Notch signaling during ommatidial development via the Roughened eye transcriptional repressor.

Squeeze and Rotund are regulated by Nab and have roles in Drosophila development
Title: Nab controls the activity of the zinc-finger transcription factors Squeeze and Rotund in Drosophila development.

The Drosophila rotund gene codes for two distinct zinc finger proteins.
Title: Control of Drosophila imaginal disc development by rotund and roughened eye: differentially expressed transcripts of the same gene encoding functionally distinct zinc finger proteins.

The following rn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04188	NM_141458.3 Latest version!	Drosophila melanogaster rotund (rn), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa04189	NM_206456.2 Latest version!	Drosophila melanogaster rotund (rn), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa07660	NM_001275414.2 Latest version!	Drosophila melanogaster rotund (rn), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa07661	NM_001144549.2 Latest version!	Drosophila melanogaster rotund (rn), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rn ( NM_141458.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649715.2 Drosophila melanogaster rotund (rn), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa04188
Clone ID Related Accession (Same CDS sequence)	NM_141458.3
Accession Version	NM_141458.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rotund, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_141458.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTATGC ATGGTACCTG GTACAAGGGA CCTCAGTTGG CGCACCATCC TCACTCGAAT 
CCGCATAACA GTTCGCACGG CCACAGTGAC TACCACCAGT TCCGCTCGTA CCCCTCGTTC 
GTTGTAACGC CCTACCACGA TGGCCATGTC CAGGGCCCGG CCACGCCCAC TCCATGCACG 
GCCCCGCCAA CGCCCTGCAA CGGTCCAGTT CCAGTTTCAG TTCCAGTTTC GATTCCCGTC 
TGCCACACCG GCGGCCAAGG TTCGCCAGTA ATTGTCGAGA GCTCATTTGC AATCGCCGCC 
GCCGCCGCTG CAGCGGCTGC TGCCGTCGCC GTCGGAGGAT CCAGTGCCAC GCCCCTGCTG 
CCCAGTCCGC CCATCAAGAT CGAGCAGGTC TTTGGCGAGC CCTCTTCGCT TGGAGCCGTC 
GTTGAGGATT ACGCGCTGGG ACTAGATTGC CCCGTGGAAC ACACGTTTCG AAAGCCGCAC 
AACAACAACG GTTACTCGTG GTCCACCGGC AACAACAACG AGGTTGTCAG TCATAGCAGC 
AACGGCCACA CCAACAACCA CCCAACAACA CCACCCACTC CGCCCAACGA GGCCAGCGCC 
ACCCAAGCGA CTTCAGTGTC CGCAATTAAT TTGAATCAAG CCCAACCCGC ATCACAGACG 
CGTTCAAACT TTGGCAGCTC CATAAAGTCA CCACCATCAG AACCGGACGC GGTCACTGCT 
GCCACGTGCA AAAGTCAGGA GAATAACTCC GGCCAGAACC CCACCCCCAA CTCCCTCTCC 
GATCACAACC CGGCTCACAA CCTTAACTCG AATTCAACGG CCGCTGCCGT GGCCGCCGCT 
GCCGCCGCCG CCGCCGCTGC CAACATGCCG ATCGGCGGCG TCCAGGGTCA GAATCCCACC 
CAGGGCCTGG TCCACTGGAT GAGTGCCGTG ATGGCCGAGC ACATGACCGG CCAGACGCAC 
CACGATCCTG GCGCCGTAGG CATGCACTAC ATGTGGAACG GGAACGTTGA CCATGCCAAA 
GACATAAGCG ATTACAATCT TTGGCCGCCA ACGCCGCGCA GTCATCAGCA TGCCAGCGAG 
CATCACCCGA TGTCCCTGAA GCAGGAGTAC GAGGCCAAAA TGAACGACCA CCATCACAAC 
AATCTGCAGA AAGGTCACTT TCTGGATGAC AATCGTCTGG AGCATCACGC GGTTACCGGA 
CAAGGTGGCC TGGGTCTGGG CGCATCCAAC GGAGTTGGCG GAGGAGGCGG TGGCGCCTCC 
GTGGTTGGAA ATTCCAGTCT GGGCGCCAGC TCCCACCACG CCGTCGCCGC CGCCCACCAT 
CACAACCAGG CGGTGGCAGC TGCTTCGGCG GCCGCTTTAC TGGTGGTGCC CCAGCCGATC 
AATGCCAGCA AAATGGGCGG ACCCGGCGGA GTCTCCTCGG TGGCGGGTGG ACATGCCACC 
GGCGGAGGGA GTGGACGCAA GTATCAGTGC AAAATGTGCC CCCAGATTTT TAGCTCGAAG 
GCAGATCTCC AGCTGCACAC GCAGATCCAC ATGCGGGAGG CGAAGCCGTA TAAGTGCACC 
CAGTGCTCGA AGGCCTTCGC CAACTCGTCG TATCTGTCGC AGCACACCCG GATCCACCTG 
GGCATCAAGC CGTACCGCTG TGAGATCTGC CAGCGAAAGT TCACGCAGCT CTCGCATTTG 
CAGCAGCACA TCCGGACGCA TACGGGCGAC AAGCCGTACA AGTGCAGGCA TCCCGGCTGC 
CAGAAGGCCT TCTCGCAGCT CTCCAACCTG CAGTCGCACT CGCGGTGCCA CCAGACGGAC 
AAGCCGTTCA AGTGCAACTC CTGCTACAAG TGCTTCTCCG ACGAGCCTTC GCTGCTGGAG 
CACATCCCCA AGCACAAGGA GTCGAAGCAC CTGAAGACGC ACATCTGCCA GTACTGCGGC 
AAGTCCTACA CCCAGGAGAC CTACCTGACC AAGCACATGC AGAAGCACGC GGAGCGGACG 
GACAAGCGGC CACCTATCGT GCCAGGCAGT GCGGCGGCCA TTGCAGCGGC GGCCGCGGCA 
GCTGCCGGCG GATCCGCCAA CCCGGCAAAT GGACCGCCTC CACCTCCCAA TCCCGCCCAG 
CATCAGCGCA ACAACCTGGG CCTGCCCCCA GTCTCGATTG CCCCCAGCGA CAACGGCTAC 
TGGCCAAAGG TAAGTCCGGA CTCGGCCGCT GCAGCCAACG CCATGGAGGT GATGCACCAG 
CAGCAGCAGC AGCAACAACA ACAGCAGCAG CAGCAGCAGC AGCAGCAGCA ACAGCAGCAG 
CAGCAGGCGC ACCACCACCA TCCGCAACAC GGAGTCCCGC CGCAGCAACA TGTCCCGCCC 
CAGCAGCAGC AGCAGCAACA ACAACAGCAG CAACACCACC ATCCGCAGCA GCAACCTCCT 
CCGCAGCACA GCATGGAAGC CCACTACGCT CAGCCGACGG CTCCCAACGG ATCCCAAAGC 
AACGGACACG GCGCGGAGTT GCAGCCCCAT CATCGCATGC CCGATCCGGT GCGGGAGGAT 
ATTGCCTCGA CCCCCAGTGC CGTGGGCCCC TATGACGCCG CATCAATAAC GAAAACCACC 
AGCAACTCGG CGTTCACGCC GATAAACTCG ATGCCACCGC ACCTGAACTC GCTGAGCCAC 
CACCCGATGG CCCAGCGGCC CTATCTCTAC GATGCCATCA GCTTCCCGAA CAAGAACGTG 
AACCAGAACA ACGCGAACGC GTTCCCCAAC CAGCTGATCT CGCTGCACCA GATCCGCAAC 
TACGCCCACC AGCCGGCGGG ACTGATGGCG GGGGAGCACC TGCTGGGCGT GAGCGTGGGT 
CCTGGGAAGG ACAAGGGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649715.2
CDS	749..3589
Translation	MIMHGTWYKGPQLAHHPHSNPHNSSHGHSDYHQFRSYPSFVVTPYHDGHVQGPATPTPCTAPPTPCNGPVPVSVPVSIPVCHTGGQGSPVIVESSFAIAAAAAAAAAAVAVGGSSATPLLPSPPIKIEQVFGEPSSLGAVVEDYALGLDCPVEHTFRKPHNNNGYSWSTGNNNEVVSHSSNGHTNNHPTTPPTPPNEASATQATSVSAINLNQAQPASQTRSNFGSSIKSPPSEPDAVTAATCKSQENNSGQNPTPNSLSDHNPAHNLNSNSTAAAVAAAAAAAAAANMPIGGVQGQNPTQGLVHWMSAVMAEHMTGQTHHDPGAVGMHYMWNGNVDHAKDISDYNLWPPTPRSHQHASEHHPMSLKQEYEAKMNDHHHNNLQKGHFLDDNRLEHHAVTGQGGLGLGASNGVGGGGGGASVVGNSSLGASSHHAVAAAHHHNQAVAAASAAALLVVPQPINASKMGGPGGVSSVAGGHATGGGSGRKYQCKMCPQIFSSKADLQLHTQIHMREAKPYKCTQCSKAFANSSYLSQHTRIHLGIKPYRCEICQRKFTQLSHLQQHIRTHTGDKPYKCRHPGCQKAFSQLSNLQSHSRCHQTDKPFKCNSCYKCFSDEPSLLEHIPKHKESKHLKTHICQYCGKSYTQETYLTKHMQKHAERTDKRPPIVPGSAAAIAAAAAAAAGGSANPANGPPPPPNPAQHQRNNLGLPPVSIAPSDNGYWPKVSPDSAAAANAMEVMHQQQQQQQQQQQQQQQQQQQQQQQAHHHHPQHGVPPQQHVPPQQQQQQQQQQQHHHPQQQPPPQHSMEAHYAQPTAPNGSQSNGHGAELQPHHRMPDPVREDIASTPSAVGPYDAASITKTTSNSAFTPINSMPPHLNSLSHHPMAQRPYLYDAISFPNKNVNQNNANAFPNQLISLHQIRNYAHQPAGLMAGEHLLGVSVGPGKDKG

Target ORF information:

RefSeq Version	NM_141458.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rotund (rn), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141458.3

ORF Insert Sequence:

ATGATTATGC ATGGTACCTG GTACAAGGGA CCTCAGTTGG CGCACCATCC TCACTCGAAT 
CCGCATAACA GTTCGCACGG CCACAGTGAC TACCACCAGT TCCGCTCGTA CCCCTCGTTC 
GTTGTAACGC CCTACCACGA TGGCCATGTC CAGGGCCCGG CCACGCCCAC TCCATGCACG 
GCCCCGCCAA CGCCCTGCAA CGGTCCAGTT CCAGTTTCAG TTCCAGTTTC GATTCCCGTC 
TGCCACACCG GCGGCCAAGG TTCGCCAGTA ATTGTCGAGA GCTCATTTGC AATCGCCGCC 
GCCGCCGCTG CAGCGGCTGC TGCCGTCGCC GTCGGAGGAT CCAGTGCCAC GCCCCTGCTG 
CCCAGTCCGC CCATCAAGAT CGAGCAGGTC TTTGGCGAGC CCTCTTCGCT TGGAGCCGTC 
GTTGAGGATT ACGCGCTGGG ACTAGATTGC CCCGTGGAAC ACACGTTTCG AAAGCCGCAC 
AACAACAACG GTTACTCGTG GTCCACCGGC AACAACAACG AGGTTGTCAG TCATAGCAGC 
AACGGCCACA CCAACAACCA CCCAACAACA CCACCCACTC CGCCCAACGA GGCCAGCGCC 
ACCCAAGCGA CTTCAGTGTC CGCAATTAAT TTGAATCAAG CCCAACCCGC ATCACAGACG 
CGTTCAAACT TTGGCAGCTC CATAAAGTCA CCACCATCAG AACCGGACGC GGTCACTGCT 
GCCACGTGCA AAAGTCAGGA GAATAACTCC GGCCAGAACC CCACCCCCAA CTCCCTCTCC 
GATCACAACC CGGCTCACAA CCTTAACTCG AATTCAACGG CCGCTGCCGT GGCCGCCGCT 
GCCGCCGCCG CCGCCGCTGC CAACATGCCG ATCGGCGGCG TCCAGGGTCA GAATCCCACC 
CAGGGCCTGG TCCACTGGAT GAGTGCCGTG ATGGCCGAGC ACATGACCGG CCAGACGCAC 
CACGATCCTG GCGCCGTAGG CATGCACTAC ATGTGGAACG GGAACGTTGA CCATGCCAAA 
GACATAAGCG ATTACAATCT TTGGCCGCCA ACGCCGCGCA GTCATCAGCA TGCCAGCGAG 
CATCACCCGA TGTCCCTGAA GCAGGAGTAC GAGGCCAAAA TGAACGACCA CCATCACAAC 
AATCTGCAGA AAGGTCACTT TCTGGATGAC AATCGTCTGG AGCATCACGC GGTTACCGGA 
CAAGGTGGCC TGGGTCTGGG CGCATCCAAC GGAGTTGGCG GAGGAGGCGG TGGCGCCTCC 
GTGGTTGGAA ATTCCAGTCT GGGCGCCAGC TCCCACCACG CCGTCGCCGC CGCCCACCAT 
CACAACCAGG CGGTGGCAGC TGCTTCGGCG GCCGCTTTAC TGGTGGTGCC CCAGCCGATC 
AATGCCAGCA AAATGGGCGG ACCCGGCGGA GTCTCCTCGG TGGCGGGTGG ACATGCCACC 
GGCGGAGGGA GTGGACGCAA GTATCAGTGC AAAATGTGCC CCCAGATTTT TAGCTCGAAG 
GCAGATCTCC AGCTGCACAC GCAGATCCAC ATGCGGGAGG CGAAGCCGTA TAAGTGCACC 
CAGTGCTCGA AGGCCTTCGC CAACTCGTCG TATCTGTCGC AGCACACCCG GATCCACCTG 
GGCATCAAGC CGTACCGCTG TGAGATCTGC CAGCGAAAGT TCACGCAGCT CTCGCATTTG 
CAGCAGCACA TCCGGACGCA TACGGGCGAC AAGCCGTACA AGTGCAGGCA TCCCGGCTGC 
CAGAAGGCCT TCTCGCAGCT CTCCAACCTG CAGTCGCACT CGCGGTGCCA CCAGACGGAC 
AAGCCGTTCA AGTGCAACTC CTGCTACAAG TGCTTCTCCG ACGAGCCTTC GCTGCTGGAG 
CACATCCCCA AGCACAAGGA GTCGAAGCAC CTGAAGACGC ACATCTGCCA GTACTGCGGC 
AAGTCCTACA CCCAGGAGAC CTACCTGACC AAGCACATGC AGAAGCACGC GGAGCGGACG 
GACAAGCGGC CACCTATCGT GCCAGGCAGT GCGGCGGCCA TTGCAGCGGC GGCCGCGGCA 
GCTGCCGGCG GATCCGCCAA CCCGGCAAAT GGACCGCCTC CACCTCCCAA TCCCGCCCAG 
CATCAGCGCA ACAACCTGGG CCTGCCCCCA GTCTCGATTG CCCCCAGCGA CAACGGCTAC 
TGGCCAAAGG TAAGTCCGGA CTCGGCCGCT GCAGCCAACG CCATGGAGGT GATGCACCAG 
CAGCAGCAGC AGCAACAACA ACAGCAGCAG CAGCAGCAGC AGCAGCAGCA ACAGCAGCAG 
CAGCAGGCGC ACCACCACCA TCCGCAACAC GGAGTCCCGC CGCAGCAACA TGTCCCGCCC 
CAGCAGCAGC AGCAGCAACA ACAACAGCAG CAACACCACC ATCCGCAGCA GCAACCTCCT 
CCGCAGCACA GCATGGAAGC CCACTACGCT CAGCCGACGG CTCCCAACGG ATCCCAAAGC 
AACGGACACG GCGCGGAGTT GCAGCCCCAT CATCGCATGC CCGATCCGGT GCGGGAGGAT 
ATTGCCTCGA CCCCCAGTGC CGTGGGCCCC TATGACGCCG CATCAATAAC GAAAACCACC 
AGCAACTCGG CGTTCACGCC GATAAACTCG ATGCCACCGC ACCTGAACTC GCTGAGCCAC 
CACCCGATGG CCCAGCGGCC CTATCTCTAC GATGCCATCA GCTTCCCGAA CAAGAACGTG 
AACCAGAACA ACGCGAACGC GTTCCCCAAC CAGCTGATCT CGCTGCACCA GATCCGCAAC 
TACGCCCACC AGCCGGCGGG ACTGATGGCG GGGGAGCACC TGCTGGGCGT GAGCGTGGGT 
CCTGGGAAGG ACAAGGGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rn ( NM_206456.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996178.1 Drosophila melanogaster rotund (rn), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa04189
Clone ID Related Accession (Same CDS sequence)	NM_206456.2
Accession Version	NM_206456.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rotund, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_206456.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAAGGCC GTCTCATCGA GTATCGTCCG ATAGGCGGCG GCCTGGACTA CCATCACAAC 
TCGCCGCTGA TTGGGGAGAT TCCGCCCGCT GGCGGGGATT ACTCCCATGC CGTGCATCGC 
TCTATCGATC ACCTGCGGAA CAGCCTCAAC GAACGCGTGG CCCTGGGTCC ACTTAGTGTG 
TTCTCCAACA CGGCCGACTT GAGGAACTCC GTCCTTAGCT ACAGCCAACA GCCCCACCAG 
CAGCCCCAGC CACCGCAGCA GCAGCAGCAA CAGCAGCAGC AGCAGCATCA CCAGCAGCAG 
CAACACCAGT ATCAGCTGGT GCCGGAGCCC AAGCCGAGTA TCACCAATCT GGAGAGCAGT 
GTGGCCTCCT CGGCGGTCAG TGGATCGGTG GCAAGTGGCC AAAAGCACGT AAACGACGCA 
GTGGTCAGCA GCAGCAGTGG GAGCAGCAGC ACCGCTGGAC CCGCCCCCAC CGGATCCACC 
AGGGGCCGCA AGTCCCGGAT CTACCCCAAT CCCAACCAGC ACATCATAGT GACCAGCAAC 
AGCGTGGACA ATGGCGGCAT CCGCATGCAG AATGCGACGT TGAACGAAAG AAATTCTCAA 
AATTCCTCTG CGGGGGCTGC TGGCGTGGGC AACGTGACCA CGGCAGCAGG ATCCGGCAAT 
GGCATATCAT CCTCCACCAG CTCGCCGCAC CACATGACCC AGTTGGACGT GAAGGACACC 
AAGATTTTTA GCTCGAAGGC AGATCTCCAG CTGCACACGC AGATCCACAT GCGGGAGGCG 
AAGCCGTATA AGTGCACCCA GTGCTCGAAG GCCTTCGCCA ACTCGTCGTA TCTGTCGCAG 
CACACCCGGA TCCACCTGGG CATCAAGCCG TACCGCTGTG AGATCTGCCA GCGAAAGTTC 
ACGCAGCTCT CGCATTTGCA GCAGCACATC CGGACGCATA CGGGCGACAA GCCGTACAAG 
TGCAGGCATC CCGGCTGCCA GAAGGCCTTC TCGCAGCTCT CCAACCTGCA GTCGCACTCG 
CGGTGCCACC AGACGGACAA GCCGTTCAAG TGCAACTCCT GCTACAAGTG CTTCTCCGAC 
GAGCCTTCGC TGCTGGAGCA CATCCCCAAG CACAAGGAGT CGAAGCACCT GAAGACGCAC 
ATCTGCCAGT ACTGCGGCAA GTCCTACACC CAGGAGACCT ACCTGACCAA GCACATGCAG 
AAGCACGCGG AGCGGACGGA CAAGCGGCCA CCTATCGTGC CAGGCAGTGC GGCGGCCATT 
GCAGCGGCGG CCGCGGCAGC TGCCGGCGGA TCCGCCAACC CGGCAAATGG ACCGCCTCCA 
CCTCCCAATC CCGCCCAGCA TCAGCGCAAC AACCTGGGCC TGCCCCCAGT CTCGATTGCC 
CCCAGCGACA ACGGCTACTG GCCAAAGGTA AGTCCGGACT CGGCCGCTGC AGCCAACGCC 
ATGGAGGTGA TGCACCAGCA GCAGCAGCAG CAACAACAAC AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAAC AGCAGCAGCA GCAGGCGCAC CACCACCATC CGCAACACGG AGTCCCGCCG 
CAGCAACATG TCCCGCCCCA GCAGCAGCAG CAGCAACAAC AACAGCAGCA ACACCACCAT 
CCGCAGCAGC AACCTCCTCC GCAGCACAGC ATGGAAGCCC ACTACGCTCA GCCGACGGCT 
CCCAACGGAT CCCAAAGCAA CGGACACGGC GCGGAGTTGC AGCCCCATCA TCGCATGCCC 
GATCCGGTGC GGGAGGATAT TGCCTCGACC CCCAGTGCCG TGGGCCCCTA TGACGCCGCA 
TCAATAACGA AAACCACCAG CAACTCGGCG TTCACGCCGA TAAACTCGAT GCCACCGCAC 
CTGAACTCGC TGAGCCACCA CCCGATGGCC CAGCGGCCCT ATCTCTACGA TGCCATCAGC 
TTCCCGAACA AGAACGTGAA CCAGAACAAC GCGAACGCGT TCCCCAACCA GCTGATCTCG 
CTGCACCAGA TCCGCAACTA CGCCCACCAG CCGGCGGGAC TGATGGCGGG GGAGCACCTG 
CTGGGCGTGA GCGTGGGTCC TGGGAAGGAC AAGGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996178.1
CDS	62..2140
Translation	MEGRLIEYRPIGGGLDYHHNSPLIGEIPPAGGDYSHAVHRSIDHLRNSLNERVALGPLSVFSNTADLRNSVLSYSQQPHQQPQPPQQQQQQQQQQHHQQQQHQYQLVPEPKPSITNLESSVASSAVSGSVASGQKHVNDAVVSSSSGSSSTAGPAPTGSTRGRKSRIYPNPNQHIIVTSNSVDNGGIRMQNATLNERNSQNSSAGAAGVGNVTTAAGSGNGISSSTSSPHHMTQLDVKDTKIFSSKADLQLHTQIHMREAKPYKCTQCSKAFANSSYLSQHTRIHLGIKPYRCEICQRKFTQLSHLQQHIRTHTGDKPYKCRHPGCQKAFSQLSNLQSHSRCHQTDKPFKCNSCYKCFSDEPSLLEHIPKHKESKHLKTHICQYCGKSYTQETYLTKHMQKHAERTDKRPPIVPGSAAAIAAAAAAAAGGSANPANGPPPPPNPAQHQRNNLGLPPVSIAPSDNGYWPKVSPDSAAAANAMEVMHQQQQQQQQQQQQQQQQQQQQQQQAHHHHPQHGVPPQQHVPPQQQQQQQQQQQHHHPQQQPPPQHSMEAHYAQPTAPNGSQSNGHGAELQPHHRMPDPVREDIASTPSAVGPYDAASITKTTSNSAFTPINSMPPHLNSLSHHPMAQRPYLYDAISFPNKNVNQNNANAFPNQLISLHQIRNYAHQPAGLMAGEHLLGVSVGPGKDKG

Target ORF information:

RefSeq Version	NM_206456.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rotund (rn), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206456.2

ORF Insert Sequence:

ATGGAAGGCC GTCTCATCGA GTATCGTCCG ATAGGCGGCG GCCTGGACTA CCATCACAAC 
TCGCCGCTGA TTGGGGAGAT TCCGCCCGCT GGCGGGGATT ACTCCCATGC CGTGCATCGC 
TCTATCGATC ACCTGCGGAA CAGCCTCAAC GAACGCGTGG CCCTGGGTCC ACTTAGTGTG 
TTCTCCAACA CGGCCGACTT GAGGAACTCC GTCCTTAGCT ACAGCCAACA GCCCCACCAG 
CAGCCCCAGC CACCGCAGCA GCAGCAGCAA CAGCAGCAGC AGCAGCATCA CCAGCAGCAG 
CAACACCAGT ATCAGCTGGT GCCGGAGCCC AAGCCGAGTA TCACCAATCT GGAGAGCAGT 
GTGGCCTCCT CGGCGGTCAG TGGATCGGTG GCAAGTGGCC AAAAGCACGT AAACGACGCA 
GTGGTCAGCA GCAGCAGTGG GAGCAGCAGC ACCGCTGGAC CCGCCCCCAC CGGATCCACC 
AGGGGCCGCA AGTCCCGGAT CTACCCCAAT CCCAACCAGC ACATCATAGT GACCAGCAAC 
AGCGTGGACA ATGGCGGCAT CCGCATGCAG AATGCGACGT TGAACGAAAG AAATTCTCAA 
AATTCCTCTG CGGGGGCTGC TGGCGTGGGC AACGTGACCA CGGCAGCAGG ATCCGGCAAT 
GGCATATCAT CCTCCACCAG CTCGCCGCAC CACATGACCC AGTTGGACGT GAAGGACACC 
AAGATTTTTA GCTCGAAGGC AGATCTCCAG CTGCACACGC AGATCCACAT GCGGGAGGCG 
AAGCCGTATA AGTGCACCCA GTGCTCGAAG GCCTTCGCCA ACTCGTCGTA TCTGTCGCAG 
CACACCCGGA TCCACCTGGG CATCAAGCCG TACCGCTGTG AGATCTGCCA GCGAAAGTTC 
ACGCAGCTCT CGCATTTGCA GCAGCACATC CGGACGCATA CGGGCGACAA GCCGTACAAG 
TGCAGGCATC CCGGCTGCCA GAAGGCCTTC TCGCAGCTCT CCAACCTGCA GTCGCACTCG 
CGGTGCCACC AGACGGACAA GCCGTTCAAG TGCAACTCCT GCTACAAGTG CTTCTCCGAC 
GAGCCTTCGC TGCTGGAGCA CATCCCCAAG CACAAGGAGT CGAAGCACCT GAAGACGCAC 
ATCTGCCAGT ACTGCGGCAA GTCCTACACC CAGGAGACCT ACCTGACCAA GCACATGCAG 
AAGCACGCGG AGCGGACGGA CAAGCGGCCA CCTATCGTGC CAGGCAGTGC GGCGGCCATT 
GCAGCGGCGG CCGCGGCAGC TGCCGGCGGA TCCGCCAACC CGGCAAATGG ACCGCCTCCA 
CCTCCCAATC CCGCCCAGCA TCAGCGCAAC AACCTGGGCC TGCCCCCAGT CTCGATTGCC 
CCCAGCGACA ACGGCTACTG GCCAAAGGTA AGTCCGGACT CGGCCGCTGC AGCCAACGCC 
ATGGAGGTGA TGCACCAGCA GCAGCAGCAG CAACAACAAC AGCAGCAGCA GCAGCAGCAG 
CAGCAGCAAC AGCAGCAGCA GCAGGCGCAC CACCACCATC CGCAACACGG AGTCCCGCCG 
CAGCAACATG TCCCGCCCCA GCAGCAGCAG CAGCAACAAC AACAGCAGCA ACACCACCAT 
CCGCAGCAGC AACCTCCTCC GCAGCACAGC ATGGAAGCCC ACTACGCTCA GCCGACGGCT 
CCCAACGGAT CCCAAAGCAA CGGACACGGC GCGGAGTTGC AGCCCCATCA TCGCATGCCC 
GATCCGGTGC GGGAGGATAT TGCCTCGACC CCCAGTGCCG TGGGCCCCTA TGACGCCGCA 
TCAATAACGA AAACCACCAG CAACTCGGCG TTCACGCCGA TAAACTCGAT GCCACCGCAC 
CTGAACTCGC TGAGCCACCA CCCGATGGCC CAGCGGCCCT ATCTCTACGA TGCCATCAGC 
TTCCCGAACA AGAACGTGAA CCAGAACAAC GCGAACGCGT TCCCCAACCA GCTGATCTCG 
CTGCACCAGA TCCGCAACTA CGCCCACCAG CCGGCGGGAC TGATGGCGGG GGAGCACCTG 
CTGGGCGTGA GCGTGGGTCC TGGGAAGGAC AAGGGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rn ( NM_001275414.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262343.1 Drosophila melanogaster rotund (rn), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa07660
Clone ID Related Accession (Same CDS sequence)	NM_001275414.2
Accession Version	NM_001275414.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2859bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rotund, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001275414.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATTATGC ATGGTACCTG GTACAAGGGA CCTCAGTTGG CGCACCATCC TCACTCGAAT 
CCGCATAACA GTTCGCACGG CCACAGTGAC TACCACCAGT TCCGCTCGTA CCCCTCGTTC 
GTTGTAACGC CCTACCACGA TGGCCATGTC CAGGGCCCGG CCACGCCCAC TCCATGCACG 
GCCCCGCCAA CGCCCTGCAA CGGTCCAGTT CCAGTTTCAG TTCCAGTTTC GATTCCCGTC 
TGCCACACCG GCGGCCAAGG TTCGCCAGTA ATTGTCGAGA GCTCATTTGC AATCGCCGCC 
GCCGCCGCTG CAGCGGCTGC TGCCGTCGCC GTCGGAGGAT CCAGTGCCAC GCCCCTGCTG 
CCCAGTCCGC CCATCAAGAT CGAGCAGGTC TTTGGCGAGC CCTCTTCGCT TGGAGCCGTC 
GTTGAGGATT ACGCGCTGGG ACTAGATTGC CCCGTGGAAC ACACGTTTCG AAAGCCGCAC 
AACAACAACG GTTACTCGTG GTCCACCGGC AACAACAACG AGGTTGTCAG TCATAGCAGC 
AACGGCCACA CCAACAACCA CCCAACAACA CCACCCACTC CGCCCAACGA GGCCAGCGCC 
ACCCAAGCGA CTTCAGTGTC CGCAATTAAT TTGAATCAAG CCCAACCCGC ATCACAGACG 
CGTTCAAACT TTGGCAGCTC CATAAAGTCA CCACCATCAG AACCGGACGC GGTCACTGCT 
GCCACGTGCA AAAGTCAGGA GAATAACTCC GGCCAGAACC CCACCCCCAA CTCCCTCTCC 
GATCACAACC CGGCTCACAA CCTTAACTCG AATTCAACGG CCGCTGCCGT GGCCGCCGCT 
GCCGCCGCCG CCGCCGCTGC CAACATGCCG ATCGGCGGCG TCCAGGGTCA GAATCCCACC 
CAGGGCCTGG TCCACTGGAT GAGTGCCGTG ATGGCCGAGC ACATGACCGG CCAGACGCAC 
CACGATCCTG GCGCCGTAGG CATGCACTAC ATGTGGAACG GGAACGTTGA CCATGCCAAA 
GACATAAGCG ATTACAATCT TTGGCCGCCA ACGCCGCGCA GTCATCAGCA TGCCAGCGAG 
CATCACCCGA TGTCCCTGAA GCAGGAGTAC GAGGCCAAAA TGAACGACCA CCATCACAAC 
AATCTGCAGA AAGGTCACTT TCTGGATGAC AATCGTCTGG AGCATCACGC GGTTACCGGA 
CAAGGTGGCC TGGGTCTGGG CGCATCCAAC GGAGTTGGCG GAGGAGGCGG TGGCGCCTCC 
GTGGTTGGAA ATTCCAGTCT GGGCGCCAGC TCCCACCACG CCGTCGCCGC CGCCCACCAT 
CACAACCAGG CGGTGGCAGC TGCTTCGGCG GCCGCTTTAC TGGTGGTGCC CCAGCCGATC 
AATGCCAGCA AAATGGGCGG ACCCGGCGGA GTCTCCTCGG TGGCGGGTGG ACATGCCACC 
GGCGGAGGGA GTGGACGCAA GTATCAGTGC AAAATGTGCC CCCAGATTTT TAGCTCGAAG 
GCAGATCTCC AGCTGCACAC GCAGATCCAC ATGCGGGAGG CGAAGCCGTA TAAGTGCACC 
CAGTGCTCGA AGGCCTTCGC CAACTCGTCG TATCTGTCGC AGCACACCCG GATCCACCTG 
GGCATCAAGC CGTACCGCTG TGAGATCTGC CAGCGAAAGT TCACGCAGCT CTCGCATTTG 
CAGCAGCACA TCCGGACGCA TACGGGCGAC AAGCCGTACA AGTGCAGGCA TCCCGGCTGC 
CAGAAGGCCT TCTCGCAGCT CTCCAACCTG CAGTCGCACT CGCGGTGCCA CCAGACGGAC 
AAGCCGTTCA AGTGCAACTC CTGCTACAAG TGCTTCTCCG ACGAGCCTTC GCTGCTGGAG 
CACATCCCCA AGCACAAGGA GTCGAAGCAC CTGAAGACGC ACATCTGCCA GTACTGCGGC 
AAGTCCTACA CCCAGGAGAC CTACCTGACC AAGCACATGC AGAAGCACGC GGAGCGGACG 
GACAAGCGGC CACCTATCGT GCCAGGCAGT GCGGCGGCCA TTGCAGCGGC GGCCGCGGCA 
GCTGCCGGCG GATCCGCCAA CCCGGCAAAT GGACCGCCTC CACCTCCCAA TCCCGCCCAG 
CATCAGCGCA ACAACCTGGG CCTGCCCCCA GTCTCGATTG CCCCCAGCGA CAACGGCTAC 
TGGCCAAAGG TAAGTCCGGA CTCGGCCGCT GCAGCCAACG CCATGGAGGT GATGCACCAG 
CAGCAGCAGC AGCAACAACA ACAGCAGCAG CAGCAGCAGC AGCAGCAGCA ACAGCAGCAG 
CAGCAGGCGC ACCACCACCA TCCGCAACAC GGAGTCCCGC CGCAGCAACA TGTCCCGCCC 
CAGCAGCAGC AGCAGCAACA ACAACAGCAG CAACACCACC ATCCGCAGCA GCAACCTCCT 
CCGCAGCACA GCATGGAAGC CCACTACGCT CAGCCGACGG CTCCCAACGG ATCCCAAAGC 
AACGGACACG GCGCGGAGTT GCAGCCCCAT CATCGCATGC CCGATCCGGT GCGGGAGGAT 
ATTGCCTCGA CCCCCAGTGC CGTGGGCCCC TATGACGCCG CATCAATAAC GAAAACCACC 
AGCAACTCGG CGTTCACGCC GATAAACTCG ATGCCACCGC ACCTGAACTC GCTGAGCCAC 
CACCCGATGG CCCAGCGGCC CTATCTCTAC GATGCCATCA GCTTCCCGAA CAAGAACGTG 
AACCAGAACA ACGCGAACGC GTTCCCCAAC CAGCTGATCT CGCTGCACCA GATCCGCAAC 
TACGCCCACC AGCCGGCGGG ACTGATGGCG GGGGAGCACC TGCTGGGCGT GAGCGTGGGT 
CCTGGGAAGG ACAAGGGATA GCTAGGGGCC TACTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262343.1
CDS	749..3607
Translation	MIMHGTWYKGPQLAHHPHSNPHNSSHGHSDYHQFRSYPSFVVTPYHDGHVQGPATPTPCTAPPTPCNGPVPVSVPVSIPVCHTGGQGSPVIVESSFAIAAAAAAAAAAVAVGGSSATPLLPSPPIKIEQVFGEPSSLGAVVEDYALGLDCPVEHTFRKPHNNNGYSWSTGNNNEVVSHSSNGHTNNHPTTPPTPPNEASATQATSVSAINLNQAQPASQTRSNFGSSIKSPPSEPDAVTAATCKSQENNSGQNPTPNSLSDHNPAHNLNSNSTAAAVAAAAAAAAAANMPIGGVQGQNPTQGLVHWMSAVMAEHMTGQTHHDPGAVGMHYMWNGNVDHAKDISDYNLWPPTPRSHQHASEHHPMSLKQEYEAKMNDHHHNNLQKGHFLDDNRLEHHAVTGQGGLGLGASNGVGGGGGGASVVGNSSLGASSHHAVAAAHHHNQAVAAASAAALLVVPQPINASKMGGPGGVSSVAGGHATGGGSGRKYQCKMCPQIFSSKADLQLHTQIHMREAKPYKCTQCSKAFANSSYLSQHTRIHLGIKPYRCEICQRKFTQLSHLQQHIRTHTGDKPYKCRHPGCQKAFSQLSNLQSHSRCHQTDKPFKCNSCYKCFSDEPSLLEHIPKHKESKHLKTHICQYCGKSYTQETYLTKHMQKHAERTDKRPPIVPGSAAAIAAAAAAAAGGSANPANGPPPPPNPAQHQRNNLGLPPVSIAPSDNGYWPKVSPDSAAAANAMEVMHQQQQQQQQQQQQQQQQQQQQQQQAHHHHPQHGVPPQQHVPPQQQQQQQQQQQHHHPQQQPPPQHSMEAHYAQPTAPNGSQSNGHGAELQPHHRMPDPVREDIASTPSAVGPYDAASITKTTSNSAFTPINSMPPHLNSLSHHPMAQRPYLYDAISFPNKNVNQNNANAFPNQLISLHQIRNYAHQPAGLMAGEHLLGVSVGPGKDKG*LGAYF

Target ORF information:

RefSeq Version	NM_001275414.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rotund (rn), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275414.2

ORF Insert Sequence:

ATGATTATGC ATGGTACCTG GTACAAGGGA CCTCAGTTGG CGCACCATCC TCACTCGAAT 
CCGCATAACA GTTCGCACGG CCACAGTGAC TACCACCAGT TCCGCTCGTA CCCCTCGTTC 
GTTGTAACGC CCTACCACGA TGGCCATGTC CAGGGCCCGG CCACGCCCAC TCCATGCACG 
GCCCCGCCAA CGCCCTGCAA CGGTCCAGTT CCAGTTTCAG TTCCAGTTTC GATTCCCGTC 
TGCCACACCG GCGGCCAAGG TTCGCCAGTA ATTGTCGAGA GCTCATTTGC AATCGCCGCC 
GCCGCCGCTG CAGCGGCTGC TGCCGTCGCC GTCGGAGGAT CCAGTGCCAC GCCCCTGCTG 
CCCAGTCCGC CCATCAAGAT CGAGCAGGTC TTTGGCGAGC CCTCTTCGCT TGGAGCCGTC 
GTTGAGGATT ACGCGCTGGG ACTAGATTGC CCCGTGGAAC ACACGTTTCG AAAGCCGCAC 
AACAACAACG GTTACTCGTG GTCCACCGGC AACAACAACG AGGTTGTCAG TCATAGCAGC 
AACGGCCACA CCAACAACCA CCCAACAACA CCACCCACTC CGCCCAACGA GGCCAGCGCC 
ACCCAAGCGA CTTCAGTGTC CGCAATTAAT TTGAATCAAG CCCAACCCGC ATCACAGACG 
CGTTCAAACT TTGGCAGCTC CATAAAGTCA CCACCATCAG AACCGGACGC GGTCACTGCT 
GCCACGTGCA AAAGTCAGGA GAATAACTCC GGCCAGAACC CCACCCCCAA CTCCCTCTCC 
GATCACAACC CGGCTCACAA CCTTAACTCG AATTCAACGG CCGCTGCCGT GGCCGCCGCT 
GCCGCCGCCG CCGCCGCTGC CAACATGCCG ATCGGCGGCG TCCAGGGTCA GAATCCCACC 
CAGGGCCTGG TCCACTGGAT GAGTGCCGTG ATGGCCGAGC ACATGACCGG CCAGACGCAC 
CACGATCCTG GCGCCGTAGG CATGCACTAC ATGTGGAACG GGAACGTTGA CCATGCCAAA 
GACATAAGCG ATTACAATCT TTGGCCGCCA ACGCCGCGCA GTCATCAGCA TGCCAGCGAG 
CATCACCCGA TGTCCCTGAA GCAGGAGTAC GAGGCCAAAA TGAACGACCA CCATCACAAC 
AATCTGCAGA AAGGTCACTT TCTGGATGAC AATCGTCTGG AGCATCACGC GGTTACCGGA 
CAAGGTGGCC TGGGTCTGGG CGCATCCAAC GGAGTTGGCG GAGGAGGCGG TGGCGCCTCC 
GTGGTTGGAA ATTCCAGTCT GGGCGCCAGC TCCCACCACG CCGTCGCCGC CGCCCACCAT 
CACAACCAGG CGGTGGCAGC TGCTTCGGCG GCCGCTTTAC TGGTGGTGCC CCAGCCGATC 
AATGCCAGCA AAATGGGCGG ACCCGGCGGA GTCTCCTCGG TGGCGGGTGG ACATGCCACC 
GGCGGAGGGA GTGGACGCAA GTATCAGTGC AAAATGTGCC CCCAGATTTT TAGCTCGAAG 
GCAGATCTCC AGCTGCACAC GCAGATCCAC ATGCGGGAGG CGAAGCCGTA TAAGTGCACC 
CAGTGCTCGA AGGCCTTCGC CAACTCGTCG TATCTGTCGC AGCACACCCG GATCCACCTG 
GGCATCAAGC CGTACCGCTG TGAGATCTGC CAGCGAAAGT TCACGCAGCT CTCGCATTTG 
CAGCAGCACA TCCGGACGCA TACGGGCGAC AAGCCGTACA AGTGCAGGCA TCCCGGCTGC 
CAGAAGGCCT TCTCGCAGCT CTCCAACCTG CAGTCGCACT CGCGGTGCCA CCAGACGGAC 
AAGCCGTTCA AGTGCAACTC CTGCTACAAG TGCTTCTCCG ACGAGCCTTC GCTGCTGGAG 
CACATCCCCA AGCACAAGGA GTCGAAGCAC CTGAAGACGC ACATCTGCCA GTACTGCGGC 
AAGTCCTACA CCCAGGAGAC CTACCTGACC AAGCACATGC AGAAGCACGC GGAGCGGACG 
GACAAGCGGC CACCTATCGT GCCAGGCAGT GCGGCGGCCA TTGCAGCGGC GGCCGCGGCA 
GCTGCCGGCG GATCCGCCAA CCCGGCAAAT GGACCGCCTC CACCTCCCAA TCCCGCCCAG 
CATCAGCGCA ACAACCTGGG CCTGCCCCCA GTCTCGATTG CCCCCAGCGA CAACGGCTAC 
TGGCCAAAGG TAAGTCCGGA CTCGGCCGCT GCAGCCAACG CCATGGAGGT GATGCACCAG 
CAGCAGCAGC AGCAACAACA ACAGCAGCAG CAGCAGCAGC AGCAGCAGCA ACAGCAGCAG 
CAGCAGGCGC ACCACCACCA TCCGCAACAC GGAGTCCCGC CGCAGCAACA TGTCCCGCCC 
CAGCAGCAGC AGCAGCAACA ACAACAGCAG CAACACCACC ATCCGCAGCA GCAACCTCCT 
CCGCAGCACA GCATGGAAGC CCACTACGCT CAGCCGACGG CTCCCAACGG ATCCCAAAGC 
AACGGACACG GCGCGGAGTT GCAGCCCCAT CATCGCATGC CCGATCCGGT GCGGGAGGAT 
ATTGCCTCGA CCCCCAGTGC CGTGGGCCCC TATGACGCCG CATCAATAAC GAAAACCACC 
AGCAACTCGG CGTTCACGCC GATAAACTCG ATGCCACCGC ACCTGAACTC GCTGAGCCAC 
CACCCGATGG CCCAGCGGCC CTATCTCTAC GATGCCATCA GCTTCCCGAA CAAGAACGTG 
AACCAGAACA ACGCGAACGC GTTCCCCAAC CAGCTGATCT CGCTGCACCA GATCCGCAAC 
TACGCCCACC AGCCGGCGGG ACTGATGGCG GGGGAGCACC TGCTGGGCGT GAGCGTGGGT 
CCTGGGAAGG ACAAGGGATA GCTAGGGGCC TACTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rn ( NM_001144549.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001138021.1 Drosophila melanogaster rotund (rn), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa07661
Clone ID Related Accession (Same CDS sequence)	NM_001144549.2
Accession Version	NM_001144549.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	rotund, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_001144549.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCACGAAC TCATTGGCAA ATACAGCCAT TCGGACATGG ACATGCTGAA GGATCAGCTG 
AAGCGTGGGG GCTACATGGG CGCCCTTGGT CCGCCCACGC CCGGCACATC CGCCCCCTCG 
CCCTTCCTGA TGCCCGCCGA ATTCTACAAA AGCCTGCTCG CCAGCGCCGT TCTGCAACAG 
CAACAGCAGC ACAAGTTCTA CGCCGGCACG CCCTCACCCA GTCCCGCCCC CAGTTTGTCA 
CCCACCTCAG GTCGCCACTT GCTGTTTTCC ACGGCAGCTG CGGCAACCGC TTGTGCTCCG 
GAGAATGGTC ACGTGAAGAC GGAGCTCGAA GAGCAGGACC AGCAGAACGG GATGCCGGAA 
ACCGTCGGAC ATGAGGAGCA GAAACCCATC ATCGAAGAGA ACCCAACGCC GAATAAGCCG 
CACAACAACA ACGGTTACTC GTGGTCCACC GGCAACAACA ACGAGGTTGT CAGTCATAGC 
AGCAACGGCC ACACCAACAA CCACCCAACA ACACCACCCA CTCCGCCCAA CGAGGCCAGC 
GCCACCCAAG CGACTTCAGT GTCCGCAATT AATTTGAATC AAGCCCAACC CGCATCACAG 
ACGCGTTCAA ACTTTGGCAG CTCCATAAAG TCACCACCAT CAGAACCGGA CGCGGTCACT 
GCTGCCACGT GCAAAAGTCA GGAGAATAAC TCCGGCCAGA ACCCCACCCC CAACTCCCTC 
TCCGATCACA ACCCGGCTCA CAACCTTAAC TCGAATTCAA CGGCCGCTGC CGTGGCCGCC 
GCTGCCGCCG CCGCCGCCGC TGCCAACATG CCGATCGGCG GCGTCCAGGG TCAGAATCCC 
ACCCAGGGCC TGGTCCACTG GATGAGTGCC GTGATGGCCG AGCACATGAC CGGCCAGACG 
CACCACGATC CTGGCGCCGT AGGCATGCAC TACATGTGGA ACGGGAACGT TGACCATGCC 
AAAGACATAA GCGATTACAA TCTTTGGCCG CCAACGCCGC GCAGTCATCA GCATGCCAGC 
GAGCATCACC CGATGTCCCT GAAGCAGGAG TACGAGGCCA AAATGAACGA CCACCATCAC 
AACAATCTGC AGAAAGGTCA CTTTCTGGAT GACAATCGTC TGGAGCATCA CGCGGTTACC 
GGACAAGGTG GCCTGGGTCT GGGCGCATCC AACGGAGTTG GCGGAGGAGG CGGTGGCGCC 
TCCGTGGTTG GAAATTCCAG TCTGGGCGCC AGCTCCCACC ACGCCGTCGC CGCCGCCCAC 
CATCACAACC AGGCGGTGGC AGCTGCTTCG GCGGCCGCTT TACTGGTGGT GCCCCAGCCG 
ATCAATGCCA GCAAAATGGG CGGACCCGGC GGAGTCTCCT CGGTGGCGGG TGGACATGCC 
ACCGGCGGAG GGAGTGGACG CAAGTATCAG TGCAAAATGT GCCCCCAGAT TTTTAGCTCG 
AAGGCAGATC TCCAGCTGCA CACGCAGATC CACATGCGGG AGGCGAAGCC GTATAAGTGC 
ACCCAGTGCT CGAAGGCCTT CGCCAACTCG TCGTATCTGT CGCAGCACAC CCGGATCCAC 
CTGGGCATCA AGCCGTACCG CTGTGAGATC TGCCAGCGAA AGTTCACGCA GCTCTCGCAT 
TTGCAGCAGC ACATCCGGAC GCATACGGGC GACAAGCCGT ACAAGTGCAG GCATCCCGGC 
TGCCAGAAGG CCTTCTCGCA GCTCTCCAAC CTGCAGTCGC ACTCGCGGTG CCACCAGACG 
GACAAGCCGT TCAAGTGCAA CTCCTGCTAC AAGTGCTTCT CCGACGAGCC TTCGCTGCTG 
GAGCACATCC CCAAGCACAA GGAGTCGAAG CACCTGAAGA CGCACATCTG CCAGTACTGC 
GGCAAGTCCT ACACCCAGGA GACCTACCTG ACCAAGCACA TGCAGAAGCA CGCGGAGCGG 
ACGGACAAGC GGCCACCTAT CGTGCCAGGC AGTGCGGCGG CCATTGCAGC GGCGGCCGCG 
GCAGCTGCCG GCGGATCCGC CAACCCGGCA AATGGACCGC CTCCACCTCC CAATCCCGCC 
CAGCATCAGC GCAACAACCT GGGCCTGCCC CCAGTCTCGA TTGCCCCCAG CGACAACGGC 
TACTGGCCAA AGGTAAGTCC GGACTCGGCC GCTGCAGCCA ACGCCATGGA GGTGATGCAC 
CAGCAGCAGC AGCAGCAACA ACAACAGCAG CAGCAGCAGC AGCAGCAGCA GCAACAGCAG 
CAGCAGCAGG CGCACCACCA CCATCCGCAA CACGGAGTCC CGCCGCAGCA ACATGTCCCG 
CCCCAGCAGC AGCAGCAGCA ACAACAACAG CAGCAACACC ACCATCCGCA GCAGCAACCT 
CCTCCGCAGC ACAGCATGGA AGCCCACTAC GCTCAGCCGA CGGCTCCCAA CGGATCCCAA 
AGCAACGGAC ACGGCGCGGA GTTGCAGCCC CATCATCGCA TGCCCGATCC GGTGCGGGAG 
GATATTGCCT CGACCCCCAG TGCCGTGGGC CCCTATGACG CCGCATCAAT AACGAAAACC 
ACCAGCAACT CGGCGTTCAC GCCGATAAAC TCGATGCCAC CGCACCTGAA CTCGCTGAGC 
CACCACCCGA TGGCCCAGCG GCCCTATCTC TACGATGCCA TCAGCTTCCC GAACAAGAAC 
GTGAACCAGA ACAACGCGAA CGCGTTCCCC AACCAGCTGA TCTCGCTGCA CCAGATCCGC 
AACTACGCCC ACCAGCCGGC GGGACTGATG GCGGGGGAGC ACCTGCTGGG CGTGAGCGTG 
GGTCCTGGGA AGGACAAGGG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138021.1
CDS	62..2845
Translation	MHELIGKYSHSDMDMLKDQLKRGGYMGALGPPTPGTSAPSPFLMPAEFYKSLLASAVLQQQQQHKFYAGTPSPSPAPSLSPTSGRHLLFSTAAAATACAPENGHVKTELEEQDQQNGMPETVGHEEQKPIIEENPTPNKPHNNNGYSWSTGNNNEVVSHSSNGHTNNHPTTPPTPPNEASATQATSVSAINLNQAQPASQTRSNFGSSIKSPPSEPDAVTAATCKSQENNSGQNPTPNSLSDHNPAHNLNSNSTAAAVAAAAAAAAAANMPIGGVQGQNPTQGLVHWMSAVMAEHMTGQTHHDPGAVGMHYMWNGNVDHAKDISDYNLWPPTPRSHQHASEHHPMSLKQEYEAKMNDHHHNNLQKGHFLDDNRLEHHAVTGQGGLGLGASNGVGGGGGGASVVGNSSLGASSHHAVAAAHHHNQAVAAASAAALLVVPQPINASKMGGPGGVSSVAGGHATGGGSGRKYQCKMCPQIFSSKADLQLHTQIHMREAKPYKCTQCSKAFANSSYLSQHTRIHLGIKPYRCEICQRKFTQLSHLQQHIRTHTGDKPYKCRHPGCQKAFSQLSNLQSHSRCHQTDKPFKCNSCYKCFSDEPSLLEHIPKHKESKHLKTHICQYCGKSYTQETYLTKHMQKHAERTDKRPPIVPGSAAAIAAAAAAAAGGSANPANGPPPPPNPAQHQRNNLGLPPVSIAPSDNGYWPKVSPDSAAAANAMEVMHQQQQQQQQQQQQQQQQQQQQQQQAHHHHPQHGVPPQQHVPPQQQQQQQQQQQHHHPQQQPPPQHSMEAHYAQPTAPNGSQSNGHGAELQPHHRMPDPVREDIASTPSAVGPYDAASITKTTSNSAFTPINSMPPHLNSLSHHPMAQRPYLYDAISFPNKNVNQNNANAFPNQLISLHQIRNYAHQPAGLMAGEHLLGVSVGPGKDKG

Target ORF information:

RefSeq Version	NM_001144549.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster rotund (rn), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144549.2

ORF Insert Sequence:

ATGCACGAAC TCATTGGCAA ATACAGCCAT TCGGACATGG ACATGCTGAA GGATCAGCTG 
AAGCGTGGGG GCTACATGGG CGCCCTTGGT CCGCCCACGC CCGGCACATC CGCCCCCTCG 
CCCTTCCTGA TGCCCGCCGA ATTCTACAAA AGCCTGCTCG CCAGCGCCGT TCTGCAACAG 
CAACAGCAGC ACAAGTTCTA CGCCGGCACG CCCTCACCCA GTCCCGCCCC CAGTTTGTCA 
CCCACCTCAG GTCGCCACTT GCTGTTTTCC ACGGCAGCTG CGGCAACCGC TTGTGCTCCG 
GAGAATGGTC ACGTGAAGAC GGAGCTCGAA GAGCAGGACC AGCAGAACGG GATGCCGGAA 
ACCGTCGGAC ATGAGGAGCA GAAACCCATC ATCGAAGAGA ACCCAACGCC GAATAAGCCG 
CACAACAACA ACGGTTACTC GTGGTCCACC GGCAACAACA ACGAGGTTGT CAGTCATAGC 
AGCAACGGCC ACACCAACAA CCACCCAACA ACACCACCCA CTCCGCCCAA CGAGGCCAGC 
GCCACCCAAG CGACTTCAGT GTCCGCAATT AATTTGAATC AAGCCCAACC CGCATCACAG 
ACGCGTTCAA ACTTTGGCAG CTCCATAAAG TCACCACCAT CAGAACCGGA CGCGGTCACT 
GCTGCCACGT GCAAAAGTCA GGAGAATAAC TCCGGCCAGA ACCCCACCCC CAACTCCCTC 
TCCGATCACA ACCCGGCTCA CAACCTTAAC TCGAATTCAA CGGCCGCTGC CGTGGCCGCC 
GCTGCCGCCG CCGCCGCCGC TGCCAACATG CCGATCGGCG GCGTCCAGGG TCAGAATCCC 
ACCCAGGGCC TGGTCCACTG GATGAGTGCC GTGATGGCCG AGCACATGAC CGGCCAGACG 
CACCACGATC CTGGCGCCGT AGGCATGCAC TACATGTGGA ACGGGAACGT TGACCATGCC 
AAAGACATAA GCGATTACAA TCTTTGGCCG CCAACGCCGC GCAGTCATCA GCATGCCAGC 
GAGCATCACC CGATGTCCCT GAAGCAGGAG TACGAGGCCA AAATGAACGA CCACCATCAC 
AACAATCTGC AGAAAGGTCA CTTTCTGGAT GACAATCGTC TGGAGCATCA CGCGGTTACC 
GGACAAGGTG GCCTGGGTCT GGGCGCATCC AACGGAGTTG GCGGAGGAGG CGGTGGCGCC 
TCCGTGGTTG GAAATTCCAG TCTGGGCGCC AGCTCCCACC ACGCCGTCGC CGCCGCCCAC 
CATCACAACC AGGCGGTGGC AGCTGCTTCG GCGGCCGCTT TACTGGTGGT GCCCCAGCCG 
ATCAATGCCA GCAAAATGGG CGGACCCGGC GGAGTCTCCT CGGTGGCGGG TGGACATGCC 
ACCGGCGGAG GGAGTGGACG CAAGTATCAG TGCAAAATGT GCCCCCAGAT TTTTAGCTCG 
AAGGCAGATC TCCAGCTGCA CACGCAGATC CACATGCGGG AGGCGAAGCC GTATAAGTGC 
ACCCAGTGCT CGAAGGCCTT CGCCAACTCG TCGTATCTGT CGCAGCACAC CCGGATCCAC 
CTGGGCATCA AGCCGTACCG CTGTGAGATC TGCCAGCGAA AGTTCACGCA GCTCTCGCAT 
TTGCAGCAGC ACATCCGGAC GCATACGGGC GACAAGCCGT ACAAGTGCAG GCATCCCGGC 
TGCCAGAAGG CCTTCTCGCA GCTCTCCAAC CTGCAGTCGC ACTCGCGGTG CCACCAGACG 
GACAAGCCGT TCAAGTGCAA CTCCTGCTAC AAGTGCTTCT CCGACGAGCC TTCGCTGCTG 
GAGCACATCC CCAAGCACAA GGAGTCGAAG CACCTGAAGA CGCACATCTG CCAGTACTGC 
GGCAAGTCCT ACACCCAGGA GACCTACCTG ACCAAGCACA TGCAGAAGCA CGCGGAGCGG 
ACGGACAAGC GGCCACCTAT CGTGCCAGGC AGTGCGGCGG CCATTGCAGC GGCGGCCGCG 
GCAGCTGCCG GCGGATCCGC CAACCCGGCA AATGGACCGC CTCCACCTCC CAATCCCGCC 
CAGCATCAGC GCAACAACCT GGGCCTGCCC CCAGTCTCGA TTGCCCCCAG CGACAACGGC 
TACTGGCCAA AGGTAAGTCC GGACTCGGCC GCTGCAGCCA ACGCCATGGA GGTGATGCAC 
CAGCAGCAGC AGCAGCAACA ACAACAGCAG CAGCAGCAGC AGCAGCAGCA GCAACAGCAG 
CAGCAGCAGG CGCACCACCA CCATCCGCAA CACGGAGTCC CGCCGCAGCA ACATGTCCCG 
CCCCAGCAGC AGCAGCAGCA ACAACAACAG CAGCAACACC ACCATCCGCA GCAGCAACCT 
CCTCCGCAGC ACAGCATGGA AGCCCACTAC GCTCAGCCGA CGGCTCCCAA CGGATCCCAA 
AGCAACGGAC ACGGCGCGGA GTTGCAGCCC CATCATCGCA TGCCCGATCC GGTGCGGGAG 
GATATTGCCT CGACCCCCAG TGCCGTGGGC CCCTATGACG CCGCATCAAT AACGAAAACC 
ACCAGCAACT CGGCGTTCAC GCCGATAAAC TCGATGCCAC CGCACCTGAA CTCGCTGAGC 
CACCACCCGA TGGCCCAGCG GCCCTATCTC TACGATGCCA TCAGCTTCCC GAACAAGAAC 
GTGAACCAGA ACAACGCGAA CGCGTTCCCC AACCAGCTGA TCTCGCTGCA CCAGATCCGC 
AACTACGCCC ACCAGCCGGC GGGACTGATG GCGGGGGAGC ACCTGCTGGG CGTGAGCGTG 
GGTCCTGGGA AGGACAAGGG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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rn cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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