sas cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	sas
Entrez Gene ID	40861
Full Name	stranded at second
Synonyms	CG2507,Dmel\CG2507,SAS,Sas,l(3)84Cd,rfd
General protein information	Preferred Names stranded at second Names CG2507 gene product from transcript CG2507-RD CG2507-PA CG2507-PB CG2507-PC CG2507-PD responsible for dominant phenotype sas-PA sas-PB sas-PC sas-PD stranded-at-second
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 84C8-84D1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275407.2	NP_001262336.1	stranded at second, isoform C
NM_169177.3	NP_731141.1	stranded at second, isoform B
NM_001275408.2	NP_001262337.1	stranded at second, isoform D
NM_057263.5	NP_476611.1	stranded at second, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

through a genetic screen in Drosophila, we identify the ligand Sas and the receptor-type tyrosine phosphatase PTP10D as the cell-surface ligand-receptor system that drives tumour-suppressive cell competition
Title: The ligand Sas and its receptor PTP10D drive tumour-suppressive cell competition.

The study of this study showed that neuronal Ptp10D restrains signaling by overexpressed glial Sas, which would otherwise produce strong glial and axonal phenotypes.
Title: Interactions between a receptor tyrosine phosphatase and a cell surface ligand regulate axon guidance and glial-neuronal communication.

The following sas gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sas cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04168	NM_001275407.2 Latest version!	Drosophila melanogaster stranded at second (sas), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa04168	NM_057263.5 Latest version!	Drosophila melanogaster stranded at second (sas), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa04169	NM_169177.3 Latest version!	Drosophila melanogaster stranded at second (sas), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa04169	NM_001275408.2 Latest version!	Drosophila melanogaster stranded at second (sas), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sas ( NM_001275407.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262336.1 Drosophila melanogaster stranded at second (sas), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa04168
Clone ID Related Accession (Same CDS sequence)	NM_001275407.2 , NM_057263.5
Accession Version	NM_001275407.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	stranded at second, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Oct 19, 2015 this sequence version replaced NM_001275407.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAACGT GTAGAAGAAG AAAAGCCTCC GGCGGCCAAT CCACGATCAA GTGGAGTAGA 
ATGTGCCTGG CCACTCTCTG CGGATTACTT TTGCTTGGCA TTCAAATTGA GCGTGCGGCG 
TCTGCGCCCG CAGGCGAAGA CGCAGCGGCC ACAACGATGC CACCTTTGGA TACCACAACA 
GATGCCCCAG ACGCCGTTGC AGCCACCACC ACTCCAGCCA CAACTGCCGC AGAACAAAGC 
AGCAGCATCA GCAGCATAAC CACCGAGGCG GCGGATGGTT CAACGACTTC CACGACGACA 
ACAACTGAGG CGGCCAACAA ATCCAATGCG ACCGAAACTG ATTTTACGAC AAATGTGCCG 
GTGGCAAGCA GCCTGCCAGA GGAGACCAGC GTGCGATCGA CGAGCATTGA ACCCATCACC 
TCCACGGAGC CCACGACAAC GCCCCGCCAG GAAACGGAGG GACCCGATCA GCACATGGTC 
TTCTCCAACA CGGAACCAGA TCAGAGCCAC ATTCAGCACA TTCCGCTGCG GGATGAGCAC 
GCCGAGAGCA GTGGCGCCGA CGATGCCACC ACCGAGATGC AACGGCAGCG TGAGCAGGAT 
CAGCAGCAGA ATGAGCTTAA TCAGATCTCT AATGAGCAGG ACGATGTGGT CAAGGATCTC 
AACAATTTCC GACATCCGGC CACGCTCATA ACGGCCAGCA ACAGCAACAG CGAGGAGAAC 
GTCGAGATCG AAAGTGACAA ACAAGTTGAG ACAACGACGA CGGCGGTGCC GGCAGCAGCA 
ACCTCCACAT CCACAGAGGC AACAGGTACA CCGCCAACAG GTACACCAGC AACATCCACA 
TCCACAGTCC CGAACGAACG CGAAGAAGAT CCCTATCATG TGCATATACT GTCCGAGAAT 
CATGATCGCC TGGCCGAACA CGAAGATTAT CAAATGCTCT CGACCAGCAC CGAGGAATCG 
TCAACAACCT CTACCACTTC GACTACTAAC AGCACCACAG AGTCGGGCAT TGTGGCTGGT 
ATTGTTGTCA GTCAGGAGAA CAAGGCAACC GCTGAGCCAT CAACTGCAAC CGAGTCTACA 
TCCATATCCA CATCCACAAC AACTGCAGCA ACAGCCGCAA CTTCAACCAC ATCGCGAGCA 
CGCGCCATGC ATATGAATGA TCCAGAAGAT GAAGCGGCCA CCACAATAAT GCCGGACAGC 
GAGTCGGTGC CAGTGATTAA CATTGTTGAA GGACAACACA TGCTGCAGCA GGAGGATCAG 
AAGGATGAGG AGGAGGAGGG GGTGGTCAAG GAGTCGGAGA GCAGTTCCAC CACCGAGGCG 
TCGACCACCA CCACCGAGCC ATCGCCATTT GTGGCCTTTG CTGGCGAGGG ACGATCGGCG 
GGTGGCGGCA ATGATATCGA GCTGTTTCTG CACCACAATG GCTCTACACA CGAGCAGCTC 
ATGGATCTTA GTGATGTCAG CATGGACGGA GATCAGAACG AGGGCAGCAG CAAAACAGAG 
AGCAGCACTA CTAGCACCAC CACGACCACT GCTCAGCCGG AAACGGAAAT GCCGAAAATT 
GTGGAGATCA CTGCCAGCGG GGATACCATG CAGCGGGAAT GCCTGGCCAA CAACAAGAGC 
TATAAGCACG GCGAGTTGAT GGAGCGGGAT TGCGACGAGC GTTGCACCTG CAACCGCGGC 
GACTGGATGT GCGAGCCAAG GTGCAGGGGA CTAAGTTATC CGCGCGGCAG CCAGCGCAGC 
ATGGCCAATC CCAACTGTCT GGAGAAGATG GTGGAGGAGG ACGAATGCTG TCGGGTGATG 
GAATGCAGTG AGCCGCAGCT GGAGCCCACG GTGGTAGCCA CAGAGGGTGC TGCACCTTCC 
ACCAATGGAA CGGGAGAATC GGCTGTGACC CTGCCCACGA CCGATGATGA AGCCACGCCC 
AAGCCCCGAA CTGATTGCCA CTACAACAGC GGTGTCTACA AATTCCGGGA GCGTCTGGAG 
ATCGGGTGCG AGCAGATCTG TCATTGTGCC GAAGGAGGCG TCATGGATTG CAGGCCACGC 
TGCCCGGAAC GGAATCACAC GCGTCTGGAC AAGTGTGTGT ATGTAAAGGA CCCGAAGGAC 
GTGTGCTGCC AACTGGAGCT CTGCGATGTC ACGCTGGACG ATCACGAACA GCAGCCAACG 
CCGCTGCAGA GCAACAACAA CGAAGATCCA GAGGAGATCG ACCCCTTCCG CTTCCAGGAG 
CAGGCTCGCG ACGCCGGAGG CGCCAAGCCC ACTTGCACAT TCAAGGGCGC AGAATATGAT 
GTGGGTCAGC AGTTCCGCGA TGGCTGCGAC CAGTTGTGCA TTTGCAATGA GCAGGGCATT 
CACTGTGCCA AGCTGGAGTG CCCCTCGAAC TTTGGCCTGG ATGTCCAGGA TCCGCACTGC 
ATACGCTGGG AACCGGTTCC GGCGGACTTC AAGCCCTCGC CGCCGAATTG CTGTCCGGAG 
AGCATGCGTT GCGTCGACAA TGGCACATGC AGTTACCAAG GCGTCCAGAT CGAGAACTGG 
TCCCCTGTCC CAGCCAACCT GACAGGTTGC GATCAGCATT GTTATTGCGA GAATGGACGG 
GTAGAGTGCA GGGCAGCTTG TCCTCCGGTT CCAGCTCTTC CTCCGGCGGA CTTGCCCTGC 
CATCCAGCCT TGGCCCGCCT GCTGCCCATT CCCGATGACG AGTGCTGCAA GCACTGGATG 
TGTGCCCCCC AAATCCCGAA AATCGGAGGT GCGGGTCAGG ACGAAGAGAC GGAAGCTACT 
TCAACCCATT CCTCGATTCC AGCAAATGGA GCCAACAACC TGCAACCTGA TATCGAAGTT 
CACACACTGG AGGCCATCGA TCCTCGTTCC ATTCGCATCG TCTTCACCGT TCCCCAGGTC 
TATGTGAACC TCCATGGACG CGTGGAGCTG CGCTACTCGA ATGGACCCAG TAACGATACA 
TCCACCTGGG AACAACAGAT TTTTGCTCCG CCCGAGGACC TCATTGCCAC ATCCCAGATG 
GAGTTCGATC TGCCCAGTCT GGAACCAAAC TCACTGTACA AGGTGAAGAT CACCTTAATC 
CTTCGTGATC TAAACTCGCA GCCCACGAGC AGTATCTACA CCGTGAAGAC GCCACCTGAA 
AGGACCATCA CTCCGCCACC TCCGTTCCCC GATTACAGGC CAGACTTCCA GGACATCTTT 
AAGAACGTTG AGGATCCAGA ACTGACGGTC AGCGAAACGA ATGCCAGCTG GTTGCAGTTG 
ACATGGAAGA AACTTGGAGA CGACCAAATG GAATATGTGG ACGGAGTTCA GCTGCGCTAC 
AAGGAACTGA CGGGCATGAT CTACTCCTCA ACGCCTCTGA TCCATCGCAC GTTGACCAGC 
TACACCATCC AGAACCTTCA GCCGGATACG GGCTACGAGA TTGGGCTGTA CTACATCCCA 
TTGGCTGGAC ATGGAGCTGA ATTGCGTGCC GGACACATGA TTAAGGTGCG AACTGCCCAG 
AAGGTGGACG TGTATGGCTT CGATGTGACC GTTAACGTAA CCAAGGTGAA GACCCAGAGT 
GTCGAGATCT CATGGAACGG AGTGCCCTAT CCGGAGGACA AGTTCGTGCA CATTTATCGT 
GCCATCTACC AGAGCGACGC TGGCAAGGAG GACTCCAGCG TCTTCAAGGT GGCCAAGCGG 
GACAGCACCA CTGGTACCCT GATCATGGAT CTCAAACCAG GCACCAAGTA CCGCCTCTGG 
CTGGAAATGT ACCTGACCAA CGGCAACACC AAGAAGAGCA ACGTTGTCAA CTTCATCACG 
AAGCCAGGTG GTCCAGCCAC TCCCGGAAAG ACTGGAAAAC TCCTAACGGC GGGAACGGAC 
CAACCCGTAG GCGATTACTA CGGCCCCCTT GTGGTGGTTT CTGTGATCGC CGCTCTGGCG 
ATCATGTCTA CTTTGGCCCT GCTACTTATT ATCACCAGGA GACGAGTTCA TCAAACGGCA 
TCCATTACGC CACCACGAAA AAGCGACGCT GCCTACGATA ATCCCTCATA CAAGGTGGAG 
ATCCAACAGG AGACTATGAA TCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262336.1
CDS	126..4172
Translation	MQTCRRRKASGGQSTIKWSRMCLATLCGLLLLGIQIERAASAPAGEDAAATTMPPLDTTTDAPDAVAATTTPATTAAEQSSSISSITTEAADGSTTSTTTTTEAANKSNATETDFTTNVPVASSLPEETSVRSTSIEPITSTEPTTTPRQETEGPDQHMVFSNTEPDQSHIQHIPLRDEHAESSGADDATTEMQRQREQDQQQNELNQISNEQDDVVKDLNNFRHPATLITASNSNSEENVEIESDKQVETTTTAVPAAATSTSTEATGTPPTGTPATSTSTVPNEREEDPYHVHILSENHDRLAEHEDYQMLSTSTEESSTTSTTSTTNSTTESGIVAGIVVSQENKATAEPSTATESTSISTSTTTAATAATSTTSRARAMHMNDPEDEAATTIMPDSESVPVINIVEGQHMLQQEDQKDEEEEGVVKESESSSTTEASTTTTEPSPFVAFAGEGRSAGGGNDIELFLHHNGSTHEQLMDLSDVSMDGDQNEGSSKTESSTTSTTTTTAQPETEMPKIVEITASGDTMQRECLANNKSYKHGELMERDCDERCTCNRGDWMCEPRCRGLSYPRGSQRSMANPNCLEKMVEEDECCRVMECSEPQLEPTVVATEGAAPSTNGTGESAVTLPTTDDEATPKPRTDCHYNSGVYKFRERLEIGCEQICHCAEGGVMDCRPRCPERNHTRLDKCVYVKDPKDVCCQLELCDVTLDDHEQQPTPLQSNNNEDPEEIDPFRFQEQARDAGGAKPTCTFKGAEYDVGQQFRDGCDQLCICNEQGIHCAKLECPSNFGLDVQDPHCIRWEPVPADFKPSPPNCCPESMRCVDNGTCSYQGVQIENWSPVPANLTGCDQHCYCENGRVECRAACPPVPALPPADLPCHPALARLLPIPDDECCKHWMCAPQIPKIGGAGQDEETEATSTHSSIPANGANNLQPDIEVHTLEAIDPRSIRIVFTVPQVYVNLHGRVELRYSNGPSNDTSTWEQQIFAPPEDLIATSQMEFDLPSLEPNSLYKVKITLILRDLNSQPTSSIYTVKTPPERTITPPPPFPDYRPDFQDIFKNVEDPELTVSETNASWLQLTWKKLGDDQMEYVDGVQLRYKELTGMIYSSTPLIHRTLTSYTIQNLQPDTGYEIGLYYIPLAGHGAELRAGHMIKVRTAQKVDVYGFDVTVNVTKVKTQSVEISWNGVPYPEDKFVHIYRAIYQSDAGKEDSSVFKVAKRDSTTGTLIMDLKPGTKYRLWLEMYLTNGNTKKSNVVNFITKPGGPATPGKTGKLLTAGTDQPVGDYYGPLVVVSVIAALAIMSTLALLLIITRRRVHQTASITPPRKSDAAYDNPSYKVEIQQETMNL

Target ORF information:

RefSeq Version	NM_001275407.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster stranded at second (sas), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275407.2

ORF Insert Sequence:

ATGCAAACGT GTAGAAGAAG AAAAGCCTCC GGCGGCCAAT CCACGATCAA GTGGAGTAGA 
ATGTGCCTGG CCACTCTCTG CGGATTACTT TTGCTTGGCA TTCAAATTGA GCGTGCGGCG 
TCTGCGCCCG CAGGCGAAGA CGCAGCGGCC ACAACGATGC CACCTTTGGA TACCACAACA 
GATGCCCCAG ACGCCGTTGC AGCCACCACC ACTCCAGCCA CAACTGCCGC AGAACAAAGC 
AGCAGCATCA GCAGCATAAC CACCGAGGCG GCGGATGGTT CAACGACTTC CACGACGACA 
ACAACTGAGG CGGCCAACAA ATCCAATGCG ACCGAAACTG ATTTTACGAC AAATGTGCCG 
GTGGCAAGCA GCCTGCCAGA GGAGACCAGC GTGCGATCGA CGAGCATTGA ACCCATCACC 
TCCACGGAGC CCACGACAAC GCCCCGCCAG GAAACGGAGG GACCCGATCA GCACATGGTC 
TTCTCCAACA CGGAACCAGA TCAGAGCCAC ATTCAGCACA TTCCGCTGCG GGATGAGCAC 
GCCGAGAGCA GTGGCGCCGA CGATGCCACC ACCGAGATGC AACGGCAGCG TGAGCAGGAT 
CAGCAGCAGA ATGAGCTTAA TCAGATCTCT AATGAGCAGG ACGATGTGGT CAAGGATCTC 
AACAATTTCC GACATCCGGC CACGCTCATA ACGGCCAGCA ACAGCAACAG CGAGGAGAAC 
GTCGAGATCG AAAGTGACAA ACAAGTTGAG ACAACGACGA CGGCGGTGCC GGCAGCAGCA 
ACCTCCACAT CCACAGAGGC AACAGGTACA CCGCCAACAG GTACACCAGC AACATCCACA 
TCCACAGTCC CGAACGAACG CGAAGAAGAT CCCTATCATG TGCATATACT GTCCGAGAAT 
CATGATCGCC TGGCCGAACA CGAAGATTAT CAAATGCTCT CGACCAGCAC CGAGGAATCG 
TCAACAACCT CTACCACTTC GACTACTAAC AGCACCACAG AGTCGGGCAT TGTGGCTGGT 
ATTGTTGTCA GTCAGGAGAA CAAGGCAACC GCTGAGCCAT CAACTGCAAC CGAGTCTACA 
TCCATATCCA CATCCACAAC AACTGCAGCA ACAGCCGCAA CTTCAACCAC ATCGCGAGCA 
CGCGCCATGC ATATGAATGA TCCAGAAGAT GAAGCGGCCA CCACAATAAT GCCGGACAGC 
GAGTCGGTGC CAGTGATTAA CATTGTTGAA GGACAACACA TGCTGCAGCA GGAGGATCAG 
AAGGATGAGG AGGAGGAGGG GGTGGTCAAG GAGTCGGAGA GCAGTTCCAC CACCGAGGCG 
TCGACCACCA CCACCGAGCC ATCGCCATTT GTGGCCTTTG CTGGCGAGGG ACGATCGGCG 
GGTGGCGGCA ATGATATCGA GCTGTTTCTG CACCACAATG GCTCTACACA CGAGCAGCTC 
ATGGATCTTA GTGATGTCAG CATGGACGGA GATCAGAACG AGGGCAGCAG CAAAACAGAG 
AGCAGCACTA CTAGCACCAC CACGACCACT GCTCAGCCGG AAACGGAAAT GCCGAAAATT 
GTGGAGATCA CTGCCAGCGG GGATACCATG CAGCGGGAAT GCCTGGCCAA CAACAAGAGC 
TATAAGCACG GCGAGTTGAT GGAGCGGGAT TGCGACGAGC GTTGCACCTG CAACCGCGGC 
GACTGGATGT GCGAGCCAAG GTGCAGGGGA CTAAGTTATC CGCGCGGCAG CCAGCGCAGC 
ATGGCCAATC CCAACTGTCT GGAGAAGATG GTGGAGGAGG ACGAATGCTG TCGGGTGATG 
GAATGCAGTG AGCCGCAGCT GGAGCCCACG GTGGTAGCCA CAGAGGGTGC TGCACCTTCC 
ACCAATGGAA CGGGAGAATC GGCTGTGACC CTGCCCACGA CCGATGATGA AGCCACGCCC 
AAGCCCCGAA CTGATTGCCA CTACAACAGC GGTGTCTACA AATTCCGGGA GCGTCTGGAG 
ATCGGGTGCG AGCAGATCTG TCATTGTGCC GAAGGAGGCG TCATGGATTG CAGGCCACGC 
TGCCCGGAAC GGAATCACAC GCGTCTGGAC AAGTGTGTGT ATGTAAAGGA CCCGAAGGAC 
GTGTGCTGCC AACTGGAGCT CTGCGATGTC ACGCTGGACG ATCACGAACA GCAGCCAACG 
CCGCTGCAGA GCAACAACAA CGAAGATCCA GAGGAGATCG ACCCCTTCCG CTTCCAGGAG 
CAGGCTCGCG ACGCCGGAGG CGCCAAGCCC ACTTGCACAT TCAAGGGCGC AGAATATGAT 
GTGGGTCAGC AGTTCCGCGA TGGCTGCGAC CAGTTGTGCA TTTGCAATGA GCAGGGCATT 
CACTGTGCCA AGCTGGAGTG CCCCTCGAAC TTTGGCCTGG ATGTCCAGGA TCCGCACTGC 
ATACGCTGGG AACCGGTTCC GGCGGACTTC AAGCCCTCGC CGCCGAATTG CTGTCCGGAG 
AGCATGCGTT GCGTCGACAA TGGCACATGC AGTTACCAAG GCGTCCAGAT CGAGAACTGG 
TCCCCTGTCC CAGCCAACCT GACAGGTTGC GATCAGCATT GTTATTGCGA GAATGGACGG 
GTAGAGTGCA GGGCAGCTTG TCCTCCGGTT CCAGCTCTTC CTCCGGCGGA CTTGCCCTGC 
CATCCAGCCT TGGCCCGCCT GCTGCCCATT CCCGATGACG AGTGCTGCAA GCACTGGATG 
TGTGCCCCCC AAATCCCGAA AATCGGAGGT GCGGGTCAGG ACGAAGAGAC GGAAGCTACT 
TCAACCCATT CCTCGATTCC AGCAAATGGA GCCAACAACC TGCAACCTGA TATCGAAGTT 
CACACACTGG AGGCCATCGA TCCTCGTTCC ATTCGCATCG TCTTCACCGT TCCCCAGGTC 
TATGTGAACC TCCATGGACG CGTGGAGCTG CGCTACTCGA ATGGACCCAG TAACGATACA 
TCCACCTGGG AACAACAGAT TTTTGCTCCG CCCGAGGACC TCATTGCCAC ATCCCAGATG 
GAGTTCGATC TGCCCAGTCT GGAACCAAAC TCACTGTACA AGGTGAAGAT CACCTTAATC 
CTTCGTGATC TAAACTCGCA GCCCACGAGC AGTATCTACA CCGTGAAGAC GCCACCTGAA 
AGGACCATCA CTCCGCCACC TCCGTTCCCC GATTACAGGC CAGACTTCCA GGACATCTTT 
AAGAACGTTG AGGATCCAGA ACTGACGGTC AGCGAAACGA ATGCCAGCTG GTTGCAGTTG 
ACATGGAAGA AACTTGGAGA CGACCAAATG GAATATGTGG ACGGAGTTCA GCTGCGCTAC 
AAGGAACTGA CGGGCATGAT CTACTCCTCA ACGCCTCTGA TCCATCGCAC GTTGACCAGC 
TACACCATCC AGAACCTTCA GCCGGATACG GGCTACGAGA TTGGGCTGTA CTACATCCCA 
TTGGCTGGAC ATGGAGCTGA ATTGCGTGCC GGACACATGA TTAAGGTGCG AACTGCCCAG 
AAGGTGGACG TGTATGGCTT CGATGTGACC GTTAACGTAA CCAAGGTGAA GACCCAGAGT 
GTCGAGATCT CATGGAACGG AGTGCCCTAT CCGGAGGACA AGTTCGTGCA CATTTATCGT 
GCCATCTACC AGAGCGACGC TGGCAAGGAG GACTCCAGCG TCTTCAAGGT GGCCAAGCGG 
GACAGCACCA CTGGTACCCT GATCATGGAT CTCAAACCAG GCACCAAGTA CCGCCTCTGG 
CTGGAAATGT ACCTGACCAA CGGCAACACC AAGAAGAGCA ACGTTGTCAA CTTCATCACG 
AAGCCAGGTG GTCCAGCCAC TCCCGGAAAG ACTGGAAAAC TCCTAACGGC GGGAACGGAC 
CAACCCGTAG GCGATTACTA CGGCCCCCTT GTGGTGGTTT CTGTGATCGC CGCTCTGGCG 
ATCATGTCTA CTTTGGCCCT GCTACTTATT ATCACCAGGA GACGAGTTCA TCAAACGGCA 
TCCATTACGC CACCACGAAA AAGCGACGCT GCCTACGATA ATCCCTCATA CAAGGTGGAG 
ATCCAACAGG AGACTATGAA TCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sas ( NM_057263.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476611.1 Drosophila melanogaster stranded at second (sas), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa04168
Clone ID Related Accession (Same CDS sequence)	NM_001275407.2 , NM_057263.5
Accession Version	NM_057263.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	stranded at second, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Oct 19, 2015 this sequence version replaced NM_057263.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAACGT GTAGAAGAAG AAAAGCCTCC GGCGGCCAAT CCACGATCAA GTGGAGTAGA 
ATGTGCCTGG CCACTCTCTG CGGATTACTT TTGCTTGGCA TTCAAATTGA GCGTGCGGCG 
TCTGCGCCCG CAGGCGAAGA CGCAGCGGCC ACAACGATGC CACCTTTGGA TACCACAACA 
GATGCCCCAG ACGCCGTTGC AGCCACCACC ACTCCAGCCA CAACTGCCGC AGAACAAAGC 
AGCAGCATCA GCAGCATAAC CACCGAGGCG GCGGATGGTT CAACGACTTC CACGACGACA 
ACAACTGAGG CGGCCAACAA ATCCAATGCG ACCGAAACTG ATTTTACGAC AAATGTGCCG 
GTGGCAAGCA GCCTGCCAGA GGAGACCAGC GTGCGATCGA CGAGCATTGA ACCCATCACC 
TCCACGGAGC CCACGACAAC GCCCCGCCAG GAAACGGAGG GACCCGATCA GCACATGGTC 
TTCTCCAACA CGGAACCAGA TCAGAGCCAC ATTCAGCACA TTCCGCTGCG GGATGAGCAC 
GCCGAGAGCA GTGGCGCCGA CGATGCCACC ACCGAGATGC AACGGCAGCG TGAGCAGGAT 
CAGCAGCAGA ATGAGCTTAA TCAGATCTCT AATGAGCAGG ACGATGTGGT CAAGGATCTC 
AACAATTTCC GACATCCGGC CACGCTCATA ACGGCCAGCA ACAGCAACAG CGAGGAGAAC 
GTCGAGATCG AAAGTGACAA ACAAGTTGAG ACAACGACGA CGGCGGTGCC GGCAGCAGCA 
ACCTCCACAT CCACAGAGGC AACAGGTACA CCGCCAACAG GTACACCAGC AACATCCACA 
TCCACAGTCC CGAACGAACG CGAAGAAGAT CCCTATCATG TGCATATACT GTCCGAGAAT 
CATGATCGCC TGGCCGAACA CGAAGATTAT CAAATGCTCT CGACCAGCAC CGAGGAATCG 
TCAACAACCT CTACCACTTC GACTACTAAC AGCACCACAG AGTCGGGCAT TGTGGCTGGT 
ATTGTTGTCA GTCAGGAGAA CAAGGCAACC GCTGAGCCAT CAACTGCAAC CGAGTCTACA 
TCCATATCCA CATCCACAAC AACTGCAGCA ACAGCCGCAA CTTCAACCAC ATCGCGAGCA 
CGCGCCATGC ATATGAATGA TCCAGAAGAT GAAGCGGCCA CCACAATAAT GCCGGACAGC 
GAGTCGGTGC CAGTGATTAA CATTGTTGAA GGACAACACA TGCTGCAGCA GGAGGATCAG 
AAGGATGAGG AGGAGGAGGG GGTGGTCAAG GAGTCGGAGA GCAGTTCCAC CACCGAGGCG 
TCGACCACCA CCACCGAGCC ATCGCCATTT GTGGCCTTTG CTGGCGAGGG ACGATCGGCG 
GGTGGCGGCA ATGATATCGA GCTGTTTCTG CACCACAATG GCTCTACACA CGAGCAGCTC 
ATGGATCTTA GTGATGTCAG CATGGACGGA GATCAGAACG AGGGCAGCAG CAAAACAGAG 
AGCAGCACTA CTAGCACCAC CACGACCACT GCTCAGCCGG AAACGGAAAT GCCGAAAATT 
GTGGAGATCA CTGCCAGCGG GGATACCATG CAGCGGGAAT GCCTGGCCAA CAACAAGAGC 
TATAAGCACG GCGAGTTGAT GGAGCGGGAT TGCGACGAGC GTTGCACCTG CAACCGCGGC 
GACTGGATGT GCGAGCCAAG GTGCAGGGGA CTAAGTTATC CGCGCGGCAG CCAGCGCAGC 
ATGGCCAATC CCAACTGTCT GGAGAAGATG GTGGAGGAGG ACGAATGCTG TCGGGTGATG 
GAATGCAGTG AGCCGCAGCT GGAGCCCACG GTGGTAGCCA CAGAGGGTGC TGCACCTTCC 
ACCAATGGAA CGGGAGAATC GGCTGTGACC CTGCCCACGA CCGATGATGA AGCCACGCCC 
AAGCCCCGAA CTGATTGCCA CTACAACAGC GGTGTCTACA AATTCCGGGA GCGTCTGGAG 
ATCGGGTGCG AGCAGATCTG TCATTGTGCC GAAGGAGGCG TCATGGATTG CAGGCCACGC 
TGCCCGGAAC GGAATCACAC GCGTCTGGAC AAGTGTGTGT ATGTAAAGGA CCCGAAGGAC 
GTGTGCTGCC AACTGGAGCT CTGCGATGTC ACGCTGGACG ATCACGAACA GCAGCCAACG 
CCGCTGCAGA GCAACAACAA CGAAGATCCA GAGGAGATCG ACCCCTTCCG CTTCCAGGAG 
CAGGCTCGCG ACGCCGGAGG CGCCAAGCCC ACTTGCACAT TCAAGGGCGC AGAATATGAT 
GTGGGTCAGC AGTTCCGCGA TGGCTGCGAC CAGTTGTGCA TTTGCAATGA GCAGGGCATT 
CACTGTGCCA AGCTGGAGTG CCCCTCGAAC TTTGGCCTGG ATGTCCAGGA TCCGCACTGC 
ATACGCTGGG AACCGGTTCC GGCGGACTTC AAGCCCTCGC CGCCGAATTG CTGTCCGGAG 
AGCATGCGTT GCGTCGACAA TGGCACATGC AGTTACCAAG GCGTCCAGAT CGAGAACTGG 
TCCCCTGTCC CAGCCAACCT GACAGGTTGC GATCAGCATT GTTATTGCGA GAATGGACGG 
GTAGAGTGCA GGGCAGCTTG TCCTCCGGTT CCAGCTCTTC CTCCGGCGGA CTTGCCCTGC 
CATCCAGCCT TGGCCCGCCT GCTGCCCATT CCCGATGACG AGTGCTGCAA GCACTGGATG 
TGTGCCCCCC AAATCCCGAA AATCGGAGGT GCGGGTCAGG ACGAAGAGAC GGAAGCTACT 
TCAACCCATT CCTCGATTCC AGCAAATGGA GCCAACAACC TGCAACCTGA TATCGAAGTT 
CACACACTGG AGGCCATCGA TCCTCGTTCC ATTCGCATCG TCTTCACCGT TCCCCAGGTC 
TATGTGAACC TCCATGGACG CGTGGAGCTG CGCTACTCGA ATGGACCCAG TAACGATACA 
TCCACCTGGG AACAACAGAT TTTTGCTCCG CCCGAGGACC TCATTGCCAC ATCCCAGATG 
GAGTTCGATC TGCCCAGTCT GGAACCAAAC TCACTGTACA AGGTGAAGAT CACCTTAATC 
CTTCGTGATC TAAACTCGCA GCCCACGAGC AGTATCTACA CCGTGAAGAC GCCACCTGAA 
AGGACCATCA CTCCGCCACC TCCGTTCCCC GATTACAGGC CAGACTTCCA GGACATCTTT 
AAGAACGTTG AGGATCCAGA ACTGACGGTC AGCGAAACGA ATGCCAGCTG GTTGCAGTTG 
ACATGGAAGA AACTTGGAGA CGACCAAATG GAATATGTGG ACGGAGTTCA GCTGCGCTAC 
AAGGAACTGA CGGGCATGAT CTACTCCTCA ACGCCTCTGA TCCATCGCAC GTTGACCAGC 
TACACCATCC AGAACCTTCA GCCGGATACG GGCTACGAGA TTGGGCTGTA CTACATCCCA 
TTGGCTGGAC ATGGAGCTGA ATTGCGTGCC GGACACATGA TTAAGGTGCG AACTGCCCAG 
AAGGTGGACG TGTATGGCTT CGATGTGACC GTTAACGTAA CCAAGGTGAA GACCCAGAGT 
GTCGAGATCT CATGGAACGG AGTGCCCTAT CCGGAGGACA AGTTCGTGCA CATTTATCGT 
GCCATCTACC AGAGCGACGC TGGCAAGGAG GACTCCAGCG TCTTCAAGGT GGCCAAGCGG 
GACAGCACCA CTGGTACCCT GATCATGGAT CTCAAACCAG GCACCAAGTA CCGCCTCTGG 
CTGGAAATGT ACCTGACCAA CGGCAACACC AAGAAGAGCA ACGTTGTCAA CTTCATCACG 
AAGCCAGGTG GTCCAGCCAC TCCCGGAAAG ACTGGAAAAC TCCTAACGGC GGGAACGGAC 
CAACCCGTAG GCGATTACTA CGGCCCCCTT GTGGTGGTTT CTGTGATCGC CGCTCTGGCG 
ATCATGTCTA CTTTGGCCCT GCTACTTATT ATCACCAGGA GACGAGTTCA TCAAACGGCA 
TCCATTACGC CACCACGAAA AAGCGACGCT GCCTACGATA ATCCCTCATA CAAGGTGGAG 
ATCCAACAGG AGACTATGAA TCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476611.1
CDS	478..4524
Translation	MQTCRRRKASGGQSTIKWSRMCLATLCGLLLLGIQIERAASAPAGEDAAATTMPPLDTTTDAPDAVAATTTPATTAAEQSSSISSITTEAADGSTTSTTTTTEAANKSNATETDFTTNVPVASSLPEETSVRSTSIEPITSTEPTTTPRQETEGPDQHMVFSNTEPDQSHIQHIPLRDEHAESSGADDATTEMQRQREQDQQQNELNQISNEQDDVVKDLNNFRHPATLITASNSNSEENVEIESDKQVETTTTAVPAAATSTSTEATGTPPTGTPATSTSTVPNEREEDPYHVHILSENHDRLAEHEDYQMLSTSTEESSTTSTTSTTNSTTESGIVAGIVVSQENKATAEPSTATESTSISTSTTTAATAATSTTSRARAMHMNDPEDEAATTIMPDSESVPVINIVEGQHMLQQEDQKDEEEEGVVKESESSSTTEASTTTTEPSPFVAFAGEGRSAGGGNDIELFLHHNGSTHEQLMDLSDVSMDGDQNEGSSKTESSTTSTTTTTAQPETEMPKIVEITASGDTMQRECLANNKSYKHGELMERDCDERCTCNRGDWMCEPRCRGLSYPRGSQRSMANPNCLEKMVEEDECCRVMECSEPQLEPTVVATEGAAPSTNGTGESAVTLPTTDDEATPKPRTDCHYNSGVYKFRERLEIGCEQICHCAEGGVMDCRPRCPERNHTRLDKCVYVKDPKDVCCQLELCDVTLDDHEQQPTPLQSNNNEDPEEIDPFRFQEQARDAGGAKPTCTFKGAEYDVGQQFRDGCDQLCICNEQGIHCAKLECPSNFGLDVQDPHCIRWEPVPADFKPSPPNCCPESMRCVDNGTCSYQGVQIENWSPVPANLTGCDQHCYCENGRVECRAACPPVPALPPADLPCHPALARLLPIPDDECCKHWMCAPQIPKIGGAGQDEETEATSTHSSIPANGANNLQPDIEVHTLEAIDPRSIRIVFTVPQVYVNLHGRVELRYSNGPSNDTSTWEQQIFAPPEDLIATSQMEFDLPSLEPNSLYKVKITLILRDLNSQPTSSIYTVKTPPERTITPPPPFPDYRPDFQDIFKNVEDPELTVSETNASWLQLTWKKLGDDQMEYVDGVQLRYKELTGMIYSSTPLIHRTLTSYTIQNLQPDTGYEIGLYYIPLAGHGAELRAGHMIKVRTAQKVDVYGFDVTVNVTKVKTQSVEISWNGVPYPEDKFVHIYRAIYQSDAGKEDSSVFKVAKRDSTTGTLIMDLKPGTKYRLWLEMYLTNGNTKKSNVVNFITKPGGPATPGKTGKLLTAGTDQPVGDYYGPLVVVSVIAALAIMSTLALLLIITRRRVHQTASITPPRKSDAAYDNPSYKVEIQQETMNL

Target ORF information:

RefSeq Version	NM_057263.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster stranded at second (sas), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057263.5

ORF Insert Sequence:

ATGCAAACGT GTAGAAGAAG AAAAGCCTCC GGCGGCCAAT CCACGATCAA GTGGAGTAGA 
ATGTGCCTGG CCACTCTCTG CGGATTACTT TTGCTTGGCA TTCAAATTGA GCGTGCGGCG 
TCTGCGCCCG CAGGCGAAGA CGCAGCGGCC ACAACGATGC CACCTTTGGA TACCACAACA 
GATGCCCCAG ACGCCGTTGC AGCCACCACC ACTCCAGCCA CAACTGCCGC AGAACAAAGC 
AGCAGCATCA GCAGCATAAC CACCGAGGCG GCGGATGGTT CAACGACTTC CACGACGACA 
ACAACTGAGG CGGCCAACAA ATCCAATGCG ACCGAAACTG ATTTTACGAC AAATGTGCCG 
GTGGCAAGCA GCCTGCCAGA GGAGACCAGC GTGCGATCGA CGAGCATTGA ACCCATCACC 
TCCACGGAGC CCACGACAAC GCCCCGCCAG GAAACGGAGG GACCCGATCA GCACATGGTC 
TTCTCCAACA CGGAACCAGA TCAGAGCCAC ATTCAGCACA TTCCGCTGCG GGATGAGCAC 
GCCGAGAGCA GTGGCGCCGA CGATGCCACC ACCGAGATGC AACGGCAGCG TGAGCAGGAT 
CAGCAGCAGA ATGAGCTTAA TCAGATCTCT AATGAGCAGG ACGATGTGGT CAAGGATCTC 
AACAATTTCC GACATCCGGC CACGCTCATA ACGGCCAGCA ACAGCAACAG CGAGGAGAAC 
GTCGAGATCG AAAGTGACAA ACAAGTTGAG ACAACGACGA CGGCGGTGCC GGCAGCAGCA 
ACCTCCACAT CCACAGAGGC AACAGGTACA CCGCCAACAG GTACACCAGC AACATCCACA 
TCCACAGTCC CGAACGAACG CGAAGAAGAT CCCTATCATG TGCATATACT GTCCGAGAAT 
CATGATCGCC TGGCCGAACA CGAAGATTAT CAAATGCTCT CGACCAGCAC CGAGGAATCG 
TCAACAACCT CTACCACTTC GACTACTAAC AGCACCACAG AGTCGGGCAT TGTGGCTGGT 
ATTGTTGTCA GTCAGGAGAA CAAGGCAACC GCTGAGCCAT CAACTGCAAC CGAGTCTACA 
TCCATATCCA CATCCACAAC AACTGCAGCA ACAGCCGCAA CTTCAACCAC ATCGCGAGCA 
CGCGCCATGC ATATGAATGA TCCAGAAGAT GAAGCGGCCA CCACAATAAT GCCGGACAGC 
GAGTCGGTGC CAGTGATTAA CATTGTTGAA GGACAACACA TGCTGCAGCA GGAGGATCAG 
AAGGATGAGG AGGAGGAGGG GGTGGTCAAG GAGTCGGAGA GCAGTTCCAC CACCGAGGCG 
TCGACCACCA CCACCGAGCC ATCGCCATTT GTGGCCTTTG CTGGCGAGGG ACGATCGGCG 
GGTGGCGGCA ATGATATCGA GCTGTTTCTG CACCACAATG GCTCTACACA CGAGCAGCTC 
ATGGATCTTA GTGATGTCAG CATGGACGGA GATCAGAACG AGGGCAGCAG CAAAACAGAG 
AGCAGCACTA CTAGCACCAC CACGACCACT GCTCAGCCGG AAACGGAAAT GCCGAAAATT 
GTGGAGATCA CTGCCAGCGG GGATACCATG CAGCGGGAAT GCCTGGCCAA CAACAAGAGC 
TATAAGCACG GCGAGTTGAT GGAGCGGGAT TGCGACGAGC GTTGCACCTG CAACCGCGGC 
GACTGGATGT GCGAGCCAAG GTGCAGGGGA CTAAGTTATC CGCGCGGCAG CCAGCGCAGC 
ATGGCCAATC CCAACTGTCT GGAGAAGATG GTGGAGGAGG ACGAATGCTG TCGGGTGATG 
GAATGCAGTG AGCCGCAGCT GGAGCCCACG GTGGTAGCCA CAGAGGGTGC TGCACCTTCC 
ACCAATGGAA CGGGAGAATC GGCTGTGACC CTGCCCACGA CCGATGATGA AGCCACGCCC 
AAGCCCCGAA CTGATTGCCA CTACAACAGC GGTGTCTACA AATTCCGGGA GCGTCTGGAG 
ATCGGGTGCG AGCAGATCTG TCATTGTGCC GAAGGAGGCG TCATGGATTG CAGGCCACGC 
TGCCCGGAAC GGAATCACAC GCGTCTGGAC AAGTGTGTGT ATGTAAAGGA CCCGAAGGAC 
GTGTGCTGCC AACTGGAGCT CTGCGATGTC ACGCTGGACG ATCACGAACA GCAGCCAACG 
CCGCTGCAGA GCAACAACAA CGAAGATCCA GAGGAGATCG ACCCCTTCCG CTTCCAGGAG 
CAGGCTCGCG ACGCCGGAGG CGCCAAGCCC ACTTGCACAT TCAAGGGCGC AGAATATGAT 
GTGGGTCAGC AGTTCCGCGA TGGCTGCGAC CAGTTGTGCA TTTGCAATGA GCAGGGCATT 
CACTGTGCCA AGCTGGAGTG CCCCTCGAAC TTTGGCCTGG ATGTCCAGGA TCCGCACTGC 
ATACGCTGGG AACCGGTTCC GGCGGACTTC AAGCCCTCGC CGCCGAATTG CTGTCCGGAG 
AGCATGCGTT GCGTCGACAA TGGCACATGC AGTTACCAAG GCGTCCAGAT CGAGAACTGG 
TCCCCTGTCC CAGCCAACCT GACAGGTTGC GATCAGCATT GTTATTGCGA GAATGGACGG 
GTAGAGTGCA GGGCAGCTTG TCCTCCGGTT CCAGCTCTTC CTCCGGCGGA CTTGCCCTGC 
CATCCAGCCT TGGCCCGCCT GCTGCCCATT CCCGATGACG AGTGCTGCAA GCACTGGATG 
TGTGCCCCCC AAATCCCGAA AATCGGAGGT GCGGGTCAGG ACGAAGAGAC GGAAGCTACT 
TCAACCCATT CCTCGATTCC AGCAAATGGA GCCAACAACC TGCAACCTGA TATCGAAGTT 
CACACACTGG AGGCCATCGA TCCTCGTTCC ATTCGCATCG TCTTCACCGT TCCCCAGGTC 
TATGTGAACC TCCATGGACG CGTGGAGCTG CGCTACTCGA ATGGACCCAG TAACGATACA 
TCCACCTGGG AACAACAGAT TTTTGCTCCG CCCGAGGACC TCATTGCCAC ATCCCAGATG 
GAGTTCGATC TGCCCAGTCT GGAACCAAAC TCACTGTACA AGGTGAAGAT CACCTTAATC 
CTTCGTGATC TAAACTCGCA GCCCACGAGC AGTATCTACA CCGTGAAGAC GCCACCTGAA 
AGGACCATCA CTCCGCCACC TCCGTTCCCC GATTACAGGC CAGACTTCCA GGACATCTTT 
AAGAACGTTG AGGATCCAGA ACTGACGGTC AGCGAAACGA ATGCCAGCTG GTTGCAGTTG 
ACATGGAAGA AACTTGGAGA CGACCAAATG GAATATGTGG ACGGAGTTCA GCTGCGCTAC 
AAGGAACTGA CGGGCATGAT CTACTCCTCA ACGCCTCTGA TCCATCGCAC GTTGACCAGC 
TACACCATCC AGAACCTTCA GCCGGATACG GGCTACGAGA TTGGGCTGTA CTACATCCCA 
TTGGCTGGAC ATGGAGCTGA ATTGCGTGCC GGACACATGA TTAAGGTGCG AACTGCCCAG 
AAGGTGGACG TGTATGGCTT CGATGTGACC GTTAACGTAA CCAAGGTGAA GACCCAGAGT 
GTCGAGATCT CATGGAACGG AGTGCCCTAT CCGGAGGACA AGTTCGTGCA CATTTATCGT 
GCCATCTACC AGAGCGACGC TGGCAAGGAG GACTCCAGCG TCTTCAAGGT GGCCAAGCGG 
GACAGCACCA CTGGTACCCT GATCATGGAT CTCAAACCAG GCACCAAGTA CCGCCTCTGG 
CTGGAAATGT ACCTGACCAA CGGCAACACC AAGAAGAGCA ACGTTGTCAA CTTCATCACG 
AAGCCAGGTG GTCCAGCCAC TCCCGGAAAG ACTGGAAAAC TCCTAACGGC GGGAACGGAC 
CAACCCGTAG GCGATTACTA CGGCCCCCTT GTGGTGGTTT CTGTGATCGC CGCTCTGGCG 
ATCATGTCTA CTTTGGCCCT GCTACTTATT ATCACCAGGA GACGAGTTCA TCAAACGGCA 
TCCATTACGC CACCACGAAA AAGCGACGCT GCCTACGATA ATCCCTCATA CAAGGTGGAG 
ATCCAACAGG AGACTATGAA TCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sas ( NM_169177.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731141.1 Drosophila melanogaster stranded at second (sas), transcript variant B, mRNA.

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CloneID	OFa04169
Clone ID Related Accession (Same CDS sequence)	NM_169177.3 , NM_001275408.2
Accession Version	NM_169177.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	stranded at second, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Oct 19, 2015 this sequence version replaced NM_169177.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAACGT GTAGAAGAAG AAAAGCCTCC GGCGGCCAAT CCACGATCAA GTGGAGTAGA 
ATGTGCCTGG CCACTCTCTG CGGATTACTT TTGCTTGGCA TTCAAATTGA GCGTGCGGCG 
TCTGCGCCCG CAGGCGAAGA CGCAGCGGCC ACAACGATGC CACCTTTGGA TACCACAACA 
GATGCCCCAG ACGCCGTTGC AGCCACCACC ACTCCAGCCA CAACTGCCGC AGAACAAAGC 
AGCAGCATCA GCAGCATAAC CACCGAGGCG GCGGATGGTT CAACGACTTC CACGACGACA 
ACAACTGAGG CGGCCAACAA ATCCAATGCG ACCGAAACTG ATTTTACGAC AAATGTGCCG 
GTGGCAAGCA GCCTGCCAGA GGAGACCAGC GTGCGATCGA CGAGCATTGA ACCCATCACC 
TCCACGGAGC CCACGACAAC GCCCCGCCAG GAAACGGAGG GACCCGATCA GCACATGGTC 
TTCTCCAACA CGGAACCAGA TCAGAGCCAC ATTCAGCACA TTCCGCTGCG GGATGAGCAC 
GCCGAGAGCA GTGGCGCCGA CGATGCCACC ACCGAGATGC AACGGCAGCG TGAGCAGGAT 
CAGCAGCAGA ATGAGCTTAA TCAGATCTCT AATGAGCAGG ACGATGTGGT CAAGGATCTC 
AACAATTTCC GACATCCGGC CACGCTCATA ACGGCCAGCA ACAGCAACAG CGAGGAGAAC 
GTCGAGATCG AAAGTGACAA ACAAGTTGAG ACAACGACGA CGGCGGTGCC GGCAGCAGCA 
ACCTCCACAT CCACAGAGGC AACAGGTACA CCGCCAACAG GTACACCAGC AACATCCACA 
TCCACAGTCC CGAACGAACG CGAAGAAGAT CCCTATCATG TGCATATACT GTCCGAGAAT 
CATGATCGCC TGGCCGAACA CGAAGATTAT CAAATGCTCT CGACCAGCAC CGAGGAATCG 
TCAACAACCT CTACCACTTC GACTACTAAC AGCACCACAG AGTCGGGCAT TGTGGCTGGT 
ATTGTTGTCA GTCAGGAGAA CAAGGCAACC GCTGAGCCAT CAACTGCAAC CGAGTCTACA 
TCCATATCCA CATCCACAAC AACTGCAGCA ACAGCCGCAA CTTCAACCAC ATCGCGAGCA 
CGCGCCATGC ATATGAATGA TCCAGAAGAT GAAGCGGCCA CCACAATAAT GCCGGACAGC 
GAGTCGGTGC CAGTGATTAA CATTGTTGAA GGACAACACA TGCTGCAGCA GGAGGATCAG 
AAGGATGAGG AGGAGGAGGG GGTGGTCAAG GAGTCGGAGA GCAGTTCCAC CACCGAGGCG 
TCGACCACCA CCACCGAGCC ATCGCCATTT GTGGCCTTTG CTGGCGAGGG ACGATCGGCG 
GGTGGCGGCA ATGATATCGA GCTGTTTCTG CACCACAATG GCTCTACACA CGAGCAGCTC 
ATGGATCTTA GTGATGTCAG CATGGACGGA GATCAGAACG AGGGCAGCAG CAAAACAGAG 
AGCAGCACTA CTAGCACCAC CACGACCACT GCTCAGCCGG AAACGGAAAT GCCGAAAATT 
GTGGAGATCA CTGCCAGCGG GGATACCATG CAGCGGGAAT GCCTGGCCAA CAACAAGAGC 
TATAAGCACG GCGAGTTGAT GGAGCGGGAT TGCGACGAGC GTTGCACCTG CAACCGCGGC 
GACTGGATGT GCGAGCCAAG GTGCAGGGGA CTAAGTTATC CGCGCGGCAG CCAGCGCAGC 
ATGGCCAATC CCAACTGTCT GGAGAAGATG GTGGAGGAGG ACGAATGCTG TCGGGTGATG 
GAATGCAGTG AGCCGCAGCT GGAGCCCACG GTGGTAGCCA CAGAGGGTGC TGCACCTTCC 
ACCAATGGAA CGGGAGAATC GGCTGTGACC CTGCCCACGA CCGATGATGA AGCCACGCCC 
AAGCCCCGAA CTGATTGCCA CTACAACAGC GGTGTCTACA AATTCCGGGA GCGTCTGGAG 
ATCGGGTGCG AGCAGATCTG TCATTGTGCC GAAGGAGGCG TCATGGATTG CAGGCCACGC 
TGCCCGGAAC GGAATCACAC GCGTCTGGAC AAGTGTGTGT ATGTAAAGGA CCCGAAGGAC 
GTGTGCTGCC AACTGGAGCT CTGCGATGTC ACGCTGGACG ATCACGAACA GCAGCCAACG 
CCGCTGCAGA GCAACAACAA CGAAGATCCA GAGGAGATCG ACCCCTTCCG CTTCCAGGAG 
CAGGCTCGCG ACGCCGGAGG CGCCAAGCCC ACTTGCACAT TCAAGGGCGC AGAATATGAT 
GTGGGTCAGC AGTTCCGCGA TGGCTGCGAC CAGTTGTGCA TTTGCAATGA GCAGGGCATT 
CACTGTGCCA AGCTGGAGTG CCCCTCGAAC TTTGGCCTGG ATGTCCAGGA TCCGCACTGC 
ATACGCTGGG AACCGGTTCC GGCGGACTTC AAGCCCTCGC CGCCGAATTG CTGTCCGGAG 
AGCATGCGTT GCGTCGACAA TGGCACATGC AGTTACCAAG GCGTCCAGAT CGAGAACTGG 
TCCCCTGTCC CAGCCAACCT GACAGGTTGC GATCAGCATT GTTATTGCGA GAATGGACGG 
GTAGAGTGCA GGGCAGCTTG TCCTCCGGTT CCAGCTCTTC CTCCGGCGGA CTTGCCCTGC 
CATCCAGCCT TGGCCCGCCT GCTGCCCATT CCCGATGACG AGTGCTGCAA GCACTGGATG 
TGTGCCCCCC AAATCCCGAA AATCGGAGGT GCGGGTCAGG ACGAAGAGAC GGAAGCTACT 
TCAACCCATT CCTCGATTCC AGCAAATGAA ACAACAACAA CGACAGCGAC AGCAAATAAA 
TCGACCAGTA TACCTAGCAA AGTACCCCAA ATCAAGAAGG ACGAGGAGAA GAGACCACCA 
GCAAGTGGCG CCTTCTATCC AACCTTGGAT GGCAAGCCAC CCAAGTCGAT TGGTGGTCTT 
GGTATCTTCG AGAAGCCGGA AAAACCAGAG AAGGCCCACA AGAAAGTGCA ACATCAACAG 
CAGCAGCATC AGCAGCAGGA GCAGCAGGAG CAGCAGCAGC ACCAGAATGA TGTTATATTC 
GACGGTGATC GCACAGAGGA GCAGGAGGAG CCTTTGCCAC CGAACGGCGG TTTTGTGCCC 
TTCCAATTCG GCCAGCAGCA TCCGCATCAG CCACATCTTG GTCCGTATGG CTTCTACAAT 
CCCGTGAAGC CCGTTTACGA GGACTATAAT CCCTATGAGC CGTACGACAT CAATCCCAAT 
GGCACACCAC AGGGCAAACC GCCTCCAGTG CCCACTAGTC AGTCCGATTT GTTCAATATA 
TTAGGTGCGG AACAACCAGG ACACCCGGTT CATCCTGGCC ATGGTGGACC CCCCCGCATT 
CATCCTGGGC AAACGCAAAA AGACAATCAC AACCTGGGAC CACAAGTTAG AATCGAACAG 
ATACTGCAGC ACCTGCAGCA AACTGTTCCA GGTGGACCAC CACCACCTCC GCCCCACCAG 
CAACACCAAT CCCTGACACC GCAGCTGCAT CCGCAGCAGC AACAAATTTC GCAGCAACAT 
CCTGGTCATT ATGTGCCCAT CGTGCACAGT GGAGTGCCGC CACCGCCACC AGGACATGGC 
ATTGCCATTG TCGATGGGCA AACAGTGGCC TATGAGAGTT ATCCTGTGAT CCCGGGACTG 
GGAGTACCAC AGCACCATCC CCAGCAGCAC CAGACGACCC CGCAGCAACA CTTGCAGCAG 
ACAATCCTGC CTAGCTCGAG CACCACCTCC GGACTCTCGA CGCAGGCTAG TGAGCACAGT 
CTGCACCAGA ACCAGGGCAA GCTGGCCAAA CAGCAGCAGT CAGGAGCCAA CAACCTGCAA 
CCTGATATCG AAGTTCACAC ACTGGAGGCC ATCGATCCTC GTTCCATTCG CATCGTCTTC 
ACCGTTCCCC AGGTCTATGT GAACCTCCAT GGACGCGTGG AGCTGCGCTA CTCGAATGGA 
CCCAGTAACG ATACATCCAC CTGGGAACAA CAGATTTTTG CTCCGCCCGA GGACCTCATT 
GCCACATCCC AGATGGAGTT CGATCTGCCC AGTCTGGAAC CAAACTCACT GTACAAGGTG 
AAGATCACCT TAATCCTTCG TGATCTAAAC TCGCAGCCCA CGAGCAGTAT CTACACCGTG 
AAGACGCCAC CTGAAAGGAC CATCACTCCG CCACCTCCGT TCCCCGATTA CAGGCCAGAC 
TTCCAGGACA TCTTTAAGAA CGTTGAGGAT CCAGAACTGA CGGTCAGCGA AACGAATGCC 
AGCTGGTTGC AGTTGACATG GAAGAAACTT GGAGACGACC AAATGGAATA TGTGGACGGA 
GTTCAGCTGC GCTACAAGGA ACTGACGGGC ATGATCTACT CCTCAACGCC TCTGATCCAT 
CGCACGTTGA CCAGCTACAC CATCCAGAAC CTTCAGCCGG ATACGGGCTA CGAGATTGGG 
CTGTACTACA TCCCATTGGC TGGACATGGA GCTGAATTGC GTGCCGGACA CATGATTAAG 
GTGCGAACTG CCCAGAAGGT GGACGTGTAT GGCTTCGATG TGACCGTTAA CGTAACCAAG 
GTGAAGACCC AGAGTGTCGA GATCTCATGG AACGGAGTGC CCTATCCGGA GGACAAGTTC 
GTGCACATTT ATCGTGCCAT CTACCAGAGC GACGCTGGCA AGGAGGACTC CAGCGTCTTC 
AAGGTGGCCA AGCGGGACAG CACCACTGGT ACCCTGATCA TGGATCTCAA ACCAGGCACC 
AAGTACCGCC TCTGGCTGGA AATGTACCTG ACCAACGGCA ACACCAAGAA GAGCAACGTT 
GTCAACTTCA TCACGAAGCC AGGTGGTCCA GCCACTCCCG GAAAGACTGG AAAACTCCTA 
ACGGCGGGAA CGGACCAACC CGTAGGCGAT TACTACGGCC CCCTTGTGGT GGTTTCTGTG 
ATCGCCGCTC TGGCGATCAT GTCTACTTTG GCCCTGCTAC TTATTATCAC CAGGAGACGA 
GTTCATCAAA CGGCATCCAT TACGCCACCA CGAAAAAGCG ACGCTGCCTA CGATAATCCC 
TCATACAAGG TGGAGATCCA ACAGGAGACT ATGAATCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731141.1
CDS	244..5325
Translation	MQTCRRRKASGGQSTIKWSRMCLATLCGLLLLGIQIERAASAPAGEDAAATTMPPLDTTTDAPDAVAATTTPATTAAEQSSSISSITTEAADGSTTSTTTTTEAANKSNATETDFTTNVPVASSLPEETSVRSTSIEPITSTEPTTTPRQETEGPDQHMVFSNTEPDQSHIQHIPLRDEHAESSGADDATTEMQRQREQDQQQNELNQISNEQDDVVKDLNNFRHPATLITASNSNSEENVEIESDKQVETTTTAVPAAATSTSTEATGTPPTGTPATSTSTVPNEREEDPYHVHILSENHDRLAEHEDYQMLSTSTEESSTTSTTSTTNSTTESGIVAGIVVSQENKATAEPSTATESTSISTSTTTAATAATSTTSRARAMHMNDPEDEAATTIMPDSESVPVINIVEGQHMLQQEDQKDEEEEGVVKESESSSTTEASTTTTEPSPFVAFAGEGRSAGGGNDIELFLHHNGSTHEQLMDLSDVSMDGDQNEGSSKTESSTTSTTTTTAQPETEMPKIVEITASGDTMQRECLANNKSYKHGELMERDCDERCTCNRGDWMCEPRCRGLSYPRGSQRSMANPNCLEKMVEEDECCRVMECSEPQLEPTVVATEGAAPSTNGTGESAVTLPTTDDEATPKPRTDCHYNSGVYKFRERLEIGCEQICHCAEGGVMDCRPRCPERNHTRLDKCVYVKDPKDVCCQLELCDVTLDDHEQQPTPLQSNNNEDPEEIDPFRFQEQARDAGGAKPTCTFKGAEYDVGQQFRDGCDQLCICNEQGIHCAKLECPSNFGLDVQDPHCIRWEPVPADFKPSPPNCCPESMRCVDNGTCSYQGVQIENWSPVPANLTGCDQHCYCENGRVECRAACPPVPALPPADLPCHPALARLLPIPDDECCKHWMCAPQIPKIGGAGQDEETEATSTHSSIPANETTTTTATANKSTSIPSKVPQIKKDEEKRPPASGAFYPTLDGKPPKSIGGLGIFEKPEKPEKAHKKVQHQQQQHQQQEQQEQQQHQNDVIFDGDRTEEQEEPLPPNGGFVPFQFGQQHPHQPHLGPYGFYNPVKPVYEDYNPYEPYDINPNGTPQGKPPPVPTSQSDLFNILGAEQPGHPVHPGHGGPPRIHPGQTQKDNHNLGPQVRIEQILQHLQQTVPGGPPPPPPHQQHQSLTPQLHPQQQQISQQHPGHYVPIVHSGVPPPPPGHGIAIVDGQTVAYESYPVIPGLGVPQHHPQQHQTTPQQHLQQTILPSSSTTSGLSTQASEHSLHQNQGKLAKQQQSGANNLQPDIEVHTLEAIDPRSIRIVFTVPQVYVNLHGRVELRYSNGPSNDTSTWEQQIFAPPEDLIATSQMEFDLPSLEPNSLYKVKITLILRDLNSQPTSSIYTVKTPPERTITPPPPFPDYRPDFQDIFKNVEDPELTVSETNASWLQLTWKKLGDDQMEYVDGVQLRYKELTGMIYSSTPLIHRTLTSYTIQNLQPDTGYEIGLYYIPLAGHGAELRAGHMIKVRTAQKVDVYGFDVTVNVTKVKTQSVEISWNGVPYPEDKFVHIYRAIYQSDAGKEDSSVFKVAKRDSTTGTLIMDLKPGTKYRLWLEMYLTNGNTKKSNVVNFITKPGGPATPGKTGKLLTAGTDQPVGDYYGPLVVVSVIAALAIMSTLALLLIITRRRVHQTASITPPRKSDAAYDNPSYKVEIQQETMNL

Target ORF information:

RefSeq Version	NM_169177.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster stranded at second (sas), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169177.3

ORF Insert Sequence:

ATGCAAACGT GTAGAAGAAG AAAAGCCTCC GGCGGCCAAT CCACGATCAA GTGGAGTAGA 
ATGTGCCTGG CCACTCTCTG CGGATTACTT TTGCTTGGCA TTCAAATTGA GCGTGCGGCG 
TCTGCGCCCG CAGGCGAAGA CGCAGCGGCC ACAACGATGC CACCTTTGGA TACCACAACA 
GATGCCCCAG ACGCCGTTGC AGCCACCACC ACTCCAGCCA CAACTGCCGC AGAACAAAGC 
AGCAGCATCA GCAGCATAAC CACCGAGGCG GCGGATGGTT CAACGACTTC CACGACGACA 
ACAACTGAGG CGGCCAACAA ATCCAATGCG ACCGAAACTG ATTTTACGAC AAATGTGCCG 
GTGGCAAGCA GCCTGCCAGA GGAGACCAGC GTGCGATCGA CGAGCATTGA ACCCATCACC 
TCCACGGAGC CCACGACAAC GCCCCGCCAG GAAACGGAGG GACCCGATCA GCACATGGTC 
TTCTCCAACA CGGAACCAGA TCAGAGCCAC ATTCAGCACA TTCCGCTGCG GGATGAGCAC 
GCCGAGAGCA GTGGCGCCGA CGATGCCACC ACCGAGATGC AACGGCAGCG TGAGCAGGAT 
CAGCAGCAGA ATGAGCTTAA TCAGATCTCT AATGAGCAGG ACGATGTGGT CAAGGATCTC 
AACAATTTCC GACATCCGGC CACGCTCATA ACGGCCAGCA ACAGCAACAG CGAGGAGAAC 
GTCGAGATCG AAAGTGACAA ACAAGTTGAG ACAACGACGA CGGCGGTGCC GGCAGCAGCA 
ACCTCCACAT CCACAGAGGC AACAGGTACA CCGCCAACAG GTACACCAGC AACATCCACA 
TCCACAGTCC CGAACGAACG CGAAGAAGAT CCCTATCATG TGCATATACT GTCCGAGAAT 
CATGATCGCC TGGCCGAACA CGAAGATTAT CAAATGCTCT CGACCAGCAC CGAGGAATCG 
TCAACAACCT CTACCACTTC GACTACTAAC AGCACCACAG AGTCGGGCAT TGTGGCTGGT 
ATTGTTGTCA GTCAGGAGAA CAAGGCAACC GCTGAGCCAT CAACTGCAAC CGAGTCTACA 
TCCATATCCA CATCCACAAC AACTGCAGCA ACAGCCGCAA CTTCAACCAC ATCGCGAGCA 
CGCGCCATGC ATATGAATGA TCCAGAAGAT GAAGCGGCCA CCACAATAAT GCCGGACAGC 
GAGTCGGTGC CAGTGATTAA CATTGTTGAA GGACAACACA TGCTGCAGCA GGAGGATCAG 
AAGGATGAGG AGGAGGAGGG GGTGGTCAAG GAGTCGGAGA GCAGTTCCAC CACCGAGGCG 
TCGACCACCA CCACCGAGCC ATCGCCATTT GTGGCCTTTG CTGGCGAGGG ACGATCGGCG 
GGTGGCGGCA ATGATATCGA GCTGTTTCTG CACCACAATG GCTCTACACA CGAGCAGCTC 
ATGGATCTTA GTGATGTCAG CATGGACGGA GATCAGAACG AGGGCAGCAG CAAAACAGAG 
AGCAGCACTA CTAGCACCAC CACGACCACT GCTCAGCCGG AAACGGAAAT GCCGAAAATT 
GTGGAGATCA CTGCCAGCGG GGATACCATG CAGCGGGAAT GCCTGGCCAA CAACAAGAGC 
TATAAGCACG GCGAGTTGAT GGAGCGGGAT TGCGACGAGC GTTGCACCTG CAACCGCGGC 
GACTGGATGT GCGAGCCAAG GTGCAGGGGA CTAAGTTATC CGCGCGGCAG CCAGCGCAGC 
ATGGCCAATC CCAACTGTCT GGAGAAGATG GTGGAGGAGG ACGAATGCTG TCGGGTGATG 
GAATGCAGTG AGCCGCAGCT GGAGCCCACG GTGGTAGCCA CAGAGGGTGC TGCACCTTCC 
ACCAATGGAA CGGGAGAATC GGCTGTGACC CTGCCCACGA CCGATGATGA AGCCACGCCC 
AAGCCCCGAA CTGATTGCCA CTACAACAGC GGTGTCTACA AATTCCGGGA GCGTCTGGAG 
ATCGGGTGCG AGCAGATCTG TCATTGTGCC GAAGGAGGCG TCATGGATTG CAGGCCACGC 
TGCCCGGAAC GGAATCACAC GCGTCTGGAC AAGTGTGTGT ATGTAAAGGA CCCGAAGGAC 
GTGTGCTGCC AACTGGAGCT CTGCGATGTC ACGCTGGACG ATCACGAACA GCAGCCAACG 
CCGCTGCAGA GCAACAACAA CGAAGATCCA GAGGAGATCG ACCCCTTCCG CTTCCAGGAG 
CAGGCTCGCG ACGCCGGAGG CGCCAAGCCC ACTTGCACAT TCAAGGGCGC AGAATATGAT 
GTGGGTCAGC AGTTCCGCGA TGGCTGCGAC CAGTTGTGCA TTTGCAATGA GCAGGGCATT 
CACTGTGCCA AGCTGGAGTG CCCCTCGAAC TTTGGCCTGG ATGTCCAGGA TCCGCACTGC 
ATACGCTGGG AACCGGTTCC GGCGGACTTC AAGCCCTCGC CGCCGAATTG CTGTCCGGAG 
AGCATGCGTT GCGTCGACAA TGGCACATGC AGTTACCAAG GCGTCCAGAT CGAGAACTGG 
TCCCCTGTCC CAGCCAACCT GACAGGTTGC GATCAGCATT GTTATTGCGA GAATGGACGG 
GTAGAGTGCA GGGCAGCTTG TCCTCCGGTT CCAGCTCTTC CTCCGGCGGA CTTGCCCTGC 
CATCCAGCCT TGGCCCGCCT GCTGCCCATT CCCGATGACG AGTGCTGCAA GCACTGGATG 
TGTGCCCCCC AAATCCCGAA AATCGGAGGT GCGGGTCAGG ACGAAGAGAC GGAAGCTACT 
TCAACCCATT CCTCGATTCC AGCAAATGAA ACAACAACAA CGACAGCGAC AGCAAATAAA 
TCGACCAGTA TACCTAGCAA AGTACCCCAA ATCAAGAAGG ACGAGGAGAA GAGACCACCA 
GCAAGTGGCG CCTTCTATCC AACCTTGGAT GGCAAGCCAC CCAAGTCGAT TGGTGGTCTT 
GGTATCTTCG AGAAGCCGGA AAAACCAGAG AAGGCCCACA AGAAAGTGCA ACATCAACAG 
CAGCAGCATC AGCAGCAGGA GCAGCAGGAG CAGCAGCAGC ACCAGAATGA TGTTATATTC 
GACGGTGATC GCACAGAGGA GCAGGAGGAG CCTTTGCCAC CGAACGGCGG TTTTGTGCCC 
TTCCAATTCG GCCAGCAGCA TCCGCATCAG CCACATCTTG GTCCGTATGG CTTCTACAAT 
CCCGTGAAGC CCGTTTACGA GGACTATAAT CCCTATGAGC CGTACGACAT CAATCCCAAT 
GGCACACCAC AGGGCAAACC GCCTCCAGTG CCCACTAGTC AGTCCGATTT GTTCAATATA 
TTAGGTGCGG AACAACCAGG ACACCCGGTT CATCCTGGCC ATGGTGGACC CCCCCGCATT 
CATCCTGGGC AAACGCAAAA AGACAATCAC AACCTGGGAC CACAAGTTAG AATCGAACAG 
ATACTGCAGC ACCTGCAGCA AACTGTTCCA GGTGGACCAC CACCACCTCC GCCCCACCAG 
CAACACCAAT CCCTGACACC GCAGCTGCAT CCGCAGCAGC AACAAATTTC GCAGCAACAT 
CCTGGTCATT ATGTGCCCAT CGTGCACAGT GGAGTGCCGC CACCGCCACC AGGACATGGC 
ATTGCCATTG TCGATGGGCA AACAGTGGCC TATGAGAGTT ATCCTGTGAT CCCGGGACTG 
GGAGTACCAC AGCACCATCC CCAGCAGCAC CAGACGACCC CGCAGCAACA CTTGCAGCAG 
ACAATCCTGC CTAGCTCGAG CACCACCTCC GGACTCTCGA CGCAGGCTAG TGAGCACAGT 
CTGCACCAGA ACCAGGGCAA GCTGGCCAAA CAGCAGCAGT CAGGAGCCAA CAACCTGCAA 
CCTGATATCG AAGTTCACAC ACTGGAGGCC ATCGATCCTC GTTCCATTCG CATCGTCTTC 
ACCGTTCCCC AGGTCTATGT GAACCTCCAT GGACGCGTGG AGCTGCGCTA CTCGAATGGA 
CCCAGTAACG ATACATCCAC CTGGGAACAA CAGATTTTTG CTCCGCCCGA GGACCTCATT 
GCCACATCCC AGATGGAGTT CGATCTGCCC AGTCTGGAAC CAAACTCACT GTACAAGGTG 
AAGATCACCT TAATCCTTCG TGATCTAAAC TCGCAGCCCA CGAGCAGTAT CTACACCGTG 
AAGACGCCAC CTGAAAGGAC CATCACTCCG CCACCTCCGT TCCCCGATTA CAGGCCAGAC 
TTCCAGGACA TCTTTAAGAA CGTTGAGGAT CCAGAACTGA CGGTCAGCGA AACGAATGCC 
AGCTGGTTGC AGTTGACATG GAAGAAACTT GGAGACGACC AAATGGAATA TGTGGACGGA 
GTTCAGCTGC GCTACAAGGA ACTGACGGGC ATGATCTACT CCTCAACGCC TCTGATCCAT 
CGCACGTTGA CCAGCTACAC CATCCAGAAC CTTCAGCCGG ATACGGGCTA CGAGATTGGG 
CTGTACTACA TCCCATTGGC TGGACATGGA GCTGAATTGC GTGCCGGACA CATGATTAAG 
GTGCGAACTG CCCAGAAGGT GGACGTGTAT GGCTTCGATG TGACCGTTAA CGTAACCAAG 
GTGAAGACCC AGAGTGTCGA GATCTCATGG AACGGAGTGC CCTATCCGGA GGACAAGTTC 
GTGCACATTT ATCGTGCCAT CTACCAGAGC GACGCTGGCA AGGAGGACTC CAGCGTCTTC 
AAGGTGGCCA AGCGGGACAG CACCACTGGT ACCCTGATCA TGGATCTCAA ACCAGGCACC 
AAGTACCGCC TCTGGCTGGA AATGTACCTG ACCAACGGCA ACACCAAGAA GAGCAACGTT 
GTCAACTTCA TCACGAAGCC AGGTGGTCCA GCCACTCCCG GAAAGACTGG AAAACTCCTA 
ACGGCGGGAA CGGACCAACC CGTAGGCGAT TACTACGGCC CCCTTGTGGT GGTTTCTGTG 
ATCGCCGCTC TGGCGATCAT GTCTACTTTG GCCCTGCTAC TTATTATCAC CAGGAGACGA 
GTTCATCAAA CGGCATCCAT TACGCCACCA CGAAAAAGCG ACGCTGCCTA CGATAATCCC 
TCATACAAGG TGGAGATCCA ACAGGAGACT ATGAATCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sas ( NM_001275408.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262337.1 Drosophila melanogaster stranded at second (sas), transcript variant D, mRNA.

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CloneID	OFa04169
Clone ID Related Accession (Same CDS sequence)	NM_169177.3 , NM_001275408.2
Accession Version	NM_001275408.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	stranded at second, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Oct 19, 2015 this sequence version replaced NM_001275408.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAAACGT GTAGAAGAAG AAAAGCCTCC GGCGGCCAAT CCACGATCAA GTGGAGTAGA 
ATGTGCCTGG CCACTCTCTG CGGATTACTT TTGCTTGGCA TTCAAATTGA GCGTGCGGCG 
TCTGCGCCCG CAGGCGAAGA CGCAGCGGCC ACAACGATGC CACCTTTGGA TACCACAACA 
GATGCCCCAG ACGCCGTTGC AGCCACCACC ACTCCAGCCA CAACTGCCGC AGAACAAAGC 
AGCAGCATCA GCAGCATAAC CACCGAGGCG GCGGATGGTT CAACGACTTC CACGACGACA 
ACAACTGAGG CGGCCAACAA ATCCAATGCG ACCGAAACTG ATTTTACGAC AAATGTGCCG 
GTGGCAAGCA GCCTGCCAGA GGAGACCAGC GTGCGATCGA CGAGCATTGA ACCCATCACC 
TCCACGGAGC CCACGACAAC GCCCCGCCAG GAAACGGAGG GACCCGATCA GCACATGGTC 
TTCTCCAACA CGGAACCAGA TCAGAGCCAC ATTCAGCACA TTCCGCTGCG GGATGAGCAC 
GCCGAGAGCA GTGGCGCCGA CGATGCCACC ACCGAGATGC AACGGCAGCG TGAGCAGGAT 
CAGCAGCAGA ATGAGCTTAA TCAGATCTCT AATGAGCAGG ACGATGTGGT CAAGGATCTC 
AACAATTTCC GACATCCGGC CACGCTCATA ACGGCCAGCA ACAGCAACAG CGAGGAGAAC 
GTCGAGATCG AAAGTGACAA ACAAGTTGAG ACAACGACGA CGGCGGTGCC GGCAGCAGCA 
ACCTCCACAT CCACAGAGGC AACAGGTACA CCGCCAACAG GTACACCAGC AACATCCACA 
TCCACAGTCC CGAACGAACG CGAAGAAGAT CCCTATCATG TGCATATACT GTCCGAGAAT 
CATGATCGCC TGGCCGAACA CGAAGATTAT CAAATGCTCT CGACCAGCAC CGAGGAATCG 
TCAACAACCT CTACCACTTC GACTACTAAC AGCACCACAG AGTCGGGCAT TGTGGCTGGT 
ATTGTTGTCA GTCAGGAGAA CAAGGCAACC GCTGAGCCAT CAACTGCAAC CGAGTCTACA 
TCCATATCCA CATCCACAAC AACTGCAGCA ACAGCCGCAA CTTCAACCAC ATCGCGAGCA 
CGCGCCATGC ATATGAATGA TCCAGAAGAT GAAGCGGCCA CCACAATAAT GCCGGACAGC 
GAGTCGGTGC CAGTGATTAA CATTGTTGAA GGACAACACA TGCTGCAGCA GGAGGATCAG 
AAGGATGAGG AGGAGGAGGG GGTGGTCAAG GAGTCGGAGA GCAGTTCCAC CACCGAGGCG 
TCGACCACCA CCACCGAGCC ATCGCCATTT GTGGCCTTTG CTGGCGAGGG ACGATCGGCG 
GGTGGCGGCA ATGATATCGA GCTGTTTCTG CACCACAATG GCTCTACACA CGAGCAGCTC 
ATGGATCTTA GTGATGTCAG CATGGACGGA GATCAGAACG AGGGCAGCAG CAAAACAGAG 
AGCAGCACTA CTAGCACCAC CACGACCACT GCTCAGCCGG AAACGGAAAT GCCGAAAATT 
GTGGAGATCA CTGCCAGCGG GGATACCATG CAGCGGGAAT GCCTGGCCAA CAACAAGAGC 
TATAAGCACG GCGAGTTGAT GGAGCGGGAT TGCGACGAGC GTTGCACCTG CAACCGCGGC 
GACTGGATGT GCGAGCCAAG GTGCAGGGGA CTAAGTTATC CGCGCGGCAG CCAGCGCAGC 
ATGGCCAATC CCAACTGTCT GGAGAAGATG GTGGAGGAGG ACGAATGCTG TCGGGTGATG 
GAATGCAGTG AGCCGCAGCT GGAGCCCACG GTGGTAGCCA CAGAGGGTGC TGCACCTTCC 
ACCAATGGAA CGGGAGAATC GGCTGTGACC CTGCCCACGA CCGATGATGA AGCCACGCCC 
AAGCCCCGAA CTGATTGCCA CTACAACAGC GGTGTCTACA AATTCCGGGA GCGTCTGGAG 
ATCGGGTGCG AGCAGATCTG TCATTGTGCC GAAGGAGGCG TCATGGATTG CAGGCCACGC 
TGCCCGGAAC GGAATCACAC GCGTCTGGAC AAGTGTGTGT ATGTAAAGGA CCCGAAGGAC 
GTGTGCTGCC AACTGGAGCT CTGCGATGTC ACGCTGGACG ATCACGAACA GCAGCCAACG 
CCGCTGCAGA GCAACAACAA CGAAGATCCA GAGGAGATCG ACCCCTTCCG CTTCCAGGAG 
CAGGCTCGCG ACGCCGGAGG CGCCAAGCCC ACTTGCACAT TCAAGGGCGC AGAATATGAT 
GTGGGTCAGC AGTTCCGCGA TGGCTGCGAC CAGTTGTGCA TTTGCAATGA GCAGGGCATT 
CACTGTGCCA AGCTGGAGTG CCCCTCGAAC TTTGGCCTGG ATGTCCAGGA TCCGCACTGC 
ATACGCTGGG AACCGGTTCC GGCGGACTTC AAGCCCTCGC CGCCGAATTG CTGTCCGGAG 
AGCATGCGTT GCGTCGACAA TGGCACATGC AGTTACCAAG GCGTCCAGAT CGAGAACTGG 
TCCCCTGTCC CAGCCAACCT GACAGGTTGC GATCAGCATT GTTATTGCGA GAATGGACGG 
GTAGAGTGCA GGGCAGCTTG TCCTCCGGTT CCAGCTCTTC CTCCGGCGGA CTTGCCCTGC 
CATCCAGCCT TGGCCCGCCT GCTGCCCATT CCCGATGACG AGTGCTGCAA GCACTGGATG 
TGTGCCCCCC AAATCCCGAA AATCGGAGGT GCGGGTCAGG ACGAAGAGAC GGAAGCTACT 
TCAACCCATT CCTCGATTCC AGCAAATGAA ACAACAACAA CGACAGCGAC AGCAAATAAA 
TCGACCAGTA TACCTAGCAA AGTACCCCAA ATCAAGAAGG ACGAGGAGAA GAGACCACCA 
GCAAGTGGCG CCTTCTATCC AACCTTGGAT GGCAAGCCAC CCAAGTCGAT TGGTGGTCTT 
GGTATCTTCG AGAAGCCGGA AAAACCAGAG AAGGCCCACA AGAAAGTGCA ACATCAACAG 
CAGCAGCATC AGCAGCAGGA GCAGCAGGAG CAGCAGCAGC ACCAGAATGA TGTTATATTC 
GACGGTGATC GCACAGAGGA GCAGGAGGAG CCTTTGCCAC CGAACGGCGG TTTTGTGCCC 
TTCCAATTCG GCCAGCAGCA TCCGCATCAG CCACATCTTG GTCCGTATGG CTTCTACAAT 
CCCGTGAAGC CCGTTTACGA GGACTATAAT CCCTATGAGC CGTACGACAT CAATCCCAAT 
GGCACACCAC AGGGCAAACC GCCTCCAGTG CCCACTAGTC AGTCCGATTT GTTCAATATA 
TTAGGTGCGG AACAACCAGG ACACCCGGTT CATCCTGGCC ATGGTGGACC CCCCCGCATT 
CATCCTGGGC AAACGCAAAA AGACAATCAC AACCTGGGAC CACAAGTTAG AATCGAACAG 
ATACTGCAGC ACCTGCAGCA AACTGTTCCA GGTGGACCAC CACCACCTCC GCCCCACCAG 
CAACACCAAT CCCTGACACC GCAGCTGCAT CCGCAGCAGC AACAAATTTC GCAGCAACAT 
CCTGGTCATT ATGTGCCCAT CGTGCACAGT GGAGTGCCGC CACCGCCACC AGGACATGGC 
ATTGCCATTG TCGATGGGCA AACAGTGGCC TATGAGAGTT ATCCTGTGAT CCCGGGACTG 
GGAGTACCAC AGCACCATCC CCAGCAGCAC CAGACGACCC CGCAGCAACA CTTGCAGCAG 
ACAATCCTGC CTAGCTCGAG CACCACCTCC GGACTCTCGA CGCAGGCTAG TGAGCACAGT 
CTGCACCAGA ACCAGGGCAA GCTGGCCAAA CAGCAGCAGT CAGGAGCCAA CAACCTGCAA 
CCTGATATCG AAGTTCACAC ACTGGAGGCC ATCGATCCTC GTTCCATTCG CATCGTCTTC 
ACCGTTCCCC AGGTCTATGT GAACCTCCAT GGACGCGTGG AGCTGCGCTA CTCGAATGGA 
CCCAGTAACG ATACATCCAC CTGGGAACAA CAGATTTTTG CTCCGCCCGA GGACCTCATT 
GCCACATCCC AGATGGAGTT CGATCTGCCC AGTCTGGAAC CAAACTCACT GTACAAGGTG 
AAGATCACCT TAATCCTTCG TGATCTAAAC TCGCAGCCCA CGAGCAGTAT CTACACCGTG 
AAGACGCCAC CTGAAAGGAC CATCACTCCG CCACCTCCGT TCCCCGATTA CAGGCCAGAC 
TTCCAGGACA TCTTTAAGAA CGTTGAGGAT CCAGAACTGA CGGTCAGCGA AACGAATGCC 
AGCTGGTTGC AGTTGACATG GAAGAAACTT GGAGACGACC AAATGGAATA TGTGGACGGA 
GTTCAGCTGC GCTACAAGGA ACTGACGGGC ATGATCTACT CCTCAACGCC TCTGATCCAT 
CGCACGTTGA CCAGCTACAC CATCCAGAAC CTTCAGCCGG ATACGGGCTA CGAGATTGGG 
CTGTACTACA TCCCATTGGC TGGACATGGA GCTGAATTGC GTGCCGGACA CATGATTAAG 
GTGCGAACTG CCCAGAAGGT GGACGTGTAT GGCTTCGATG TGACCGTTAA CGTAACCAAG 
GTGAAGACCC AGAGTGTCGA GATCTCATGG AACGGAGTGC CCTATCCGGA GGACAAGTTC 
GTGCACATTT ATCGTGCCAT CTACCAGAGC GACGCTGGCA AGGAGGACTC CAGCGTCTTC 
AAGGTGGCCA AGCGGGACAG CACCACTGGT ACCCTGATCA TGGATCTCAA ACCAGGCACC 
AAGTACCGCC TCTGGCTGGA AATGTACCTG ACCAACGGCA ACACCAAGAA GAGCAACGTT 
GTCAACTTCA TCACGAAGCC AGGTGGTCCA GCCACTCCCG GAAAGACTGG AAAACTCCTA 
ACGGCGGGAA CGGACCAACC CGTAGGCGAT TACTACGGCC CCCTTGTGGT GGTTTCTGTG 
ATCGCCGCTC TGGCGATCAT GTCTACTTTG GCCCTGCTAC TTATTATCAC CAGGAGACGA 
GTTCATCAAA CGGCATCCAT TACGCCACCA CGAAAAAGCG ACGCTGCCTA CGATAATCCC 
TCATACAAGG TGGAGATCCA ACAGGAGACT ATGAATCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262337.1
CDS	564..5645
Translation	MQTCRRRKASGGQSTIKWSRMCLATLCGLLLLGIQIERAASAPAGEDAAATTMPPLDTTTDAPDAVAATTTPATTAAEQSSSISSITTEAADGSTTSTTTTTEAANKSNATETDFTTNVPVASSLPEETSVRSTSIEPITSTEPTTTPRQETEGPDQHMVFSNTEPDQSHIQHIPLRDEHAESSGADDATTEMQRQREQDQQQNELNQISNEQDDVVKDLNNFRHPATLITASNSNSEENVEIESDKQVETTTTAVPAAATSTSTEATGTPPTGTPATSTSTVPNEREEDPYHVHILSENHDRLAEHEDYQMLSTSTEESSTTSTTSTTNSTTESGIVAGIVVSQENKATAEPSTATESTSISTSTTTAATAATSTTSRARAMHMNDPEDEAATTIMPDSESVPVINIVEGQHMLQQEDQKDEEEEGVVKESESSSTTEASTTTTEPSPFVAFAGEGRSAGGGNDIELFLHHNGSTHEQLMDLSDVSMDGDQNEGSSKTESSTTSTTTTTAQPETEMPKIVEITASGDTMQRECLANNKSYKHGELMERDCDERCTCNRGDWMCEPRCRGLSYPRGSQRSMANPNCLEKMVEEDECCRVMECSEPQLEPTVVATEGAAPSTNGTGESAVTLPTTDDEATPKPRTDCHYNSGVYKFRERLEIGCEQICHCAEGGVMDCRPRCPERNHTRLDKCVYVKDPKDVCCQLELCDVTLDDHEQQPTPLQSNNNEDPEEIDPFRFQEQARDAGGAKPTCTFKGAEYDVGQQFRDGCDQLCICNEQGIHCAKLECPSNFGLDVQDPHCIRWEPVPADFKPSPPNCCPESMRCVDNGTCSYQGVQIENWSPVPANLTGCDQHCYCENGRVECRAACPPVPALPPADLPCHPALARLLPIPDDECCKHWMCAPQIPKIGGAGQDEETEATSTHSSIPANETTTTTATANKSTSIPSKVPQIKKDEEKRPPASGAFYPTLDGKPPKSIGGLGIFEKPEKPEKAHKKVQHQQQQHQQQEQQEQQQHQNDVIFDGDRTEEQEEPLPPNGGFVPFQFGQQHPHQPHLGPYGFYNPVKPVYEDYNPYEPYDINPNGTPQGKPPPVPTSQSDLFNILGAEQPGHPVHPGHGGPPRIHPGQTQKDNHNLGPQVRIEQILQHLQQTVPGGPPPPPPHQQHQSLTPQLHPQQQQISQQHPGHYVPIVHSGVPPPPPGHGIAIVDGQTVAYESYPVIPGLGVPQHHPQQHQTTPQQHLQQTILPSSSTTSGLSTQASEHSLHQNQGKLAKQQQSGANNLQPDIEVHTLEAIDPRSIRIVFTVPQVYVNLHGRVELRYSNGPSNDTSTWEQQIFAPPEDLIATSQMEFDLPSLEPNSLYKVKITLILRDLNSQPTSSIYTVKTPPERTITPPPPFPDYRPDFQDIFKNVEDPELTVSETNASWLQLTWKKLGDDQMEYVDGVQLRYKELTGMIYSSTPLIHRTLTSYTIQNLQPDTGYEIGLYYIPLAGHGAELRAGHMIKVRTAQKVDVYGFDVTVNVTKVKTQSVEISWNGVPYPEDKFVHIYRAIYQSDAGKEDSSVFKVAKRDSTTGTLIMDLKPGTKYRLWLEMYLTNGNTKKSNVVNFITKPGGPATPGKTGKLLTAGTDQPVGDYYGPLVVVSVIAALAIMSTLALLLIITRRRVHQTASITPPRKSDAAYDNPSYKVEIQQETMNL

Target ORF information:

RefSeq Version	NM_001275408.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster stranded at second (sas), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275408.2

ORF Insert Sequence:

ATGCAAACGT GTAGAAGAAG AAAAGCCTCC GGCGGCCAAT CCACGATCAA GTGGAGTAGA 
ATGTGCCTGG CCACTCTCTG CGGATTACTT TTGCTTGGCA TTCAAATTGA GCGTGCGGCG 
TCTGCGCCCG CAGGCGAAGA CGCAGCGGCC ACAACGATGC CACCTTTGGA TACCACAACA 
GATGCCCCAG ACGCCGTTGC AGCCACCACC ACTCCAGCCA CAACTGCCGC AGAACAAAGC 
AGCAGCATCA GCAGCATAAC CACCGAGGCG GCGGATGGTT CAACGACTTC CACGACGACA 
ACAACTGAGG CGGCCAACAA ATCCAATGCG ACCGAAACTG ATTTTACGAC AAATGTGCCG 
GTGGCAAGCA GCCTGCCAGA GGAGACCAGC GTGCGATCGA CGAGCATTGA ACCCATCACC 
TCCACGGAGC CCACGACAAC GCCCCGCCAG GAAACGGAGG GACCCGATCA GCACATGGTC 
TTCTCCAACA CGGAACCAGA TCAGAGCCAC ATTCAGCACA TTCCGCTGCG GGATGAGCAC 
GCCGAGAGCA GTGGCGCCGA CGATGCCACC ACCGAGATGC AACGGCAGCG TGAGCAGGAT 
CAGCAGCAGA ATGAGCTTAA TCAGATCTCT AATGAGCAGG ACGATGTGGT CAAGGATCTC 
AACAATTTCC GACATCCGGC CACGCTCATA ACGGCCAGCA ACAGCAACAG CGAGGAGAAC 
GTCGAGATCG AAAGTGACAA ACAAGTTGAG ACAACGACGA CGGCGGTGCC GGCAGCAGCA 
ACCTCCACAT CCACAGAGGC AACAGGTACA CCGCCAACAG GTACACCAGC AACATCCACA 
TCCACAGTCC CGAACGAACG CGAAGAAGAT CCCTATCATG TGCATATACT GTCCGAGAAT 
CATGATCGCC TGGCCGAACA CGAAGATTAT CAAATGCTCT CGACCAGCAC CGAGGAATCG 
TCAACAACCT CTACCACTTC GACTACTAAC AGCACCACAG AGTCGGGCAT TGTGGCTGGT 
ATTGTTGTCA GTCAGGAGAA CAAGGCAACC GCTGAGCCAT CAACTGCAAC CGAGTCTACA 
TCCATATCCA CATCCACAAC AACTGCAGCA ACAGCCGCAA CTTCAACCAC ATCGCGAGCA 
CGCGCCATGC ATATGAATGA TCCAGAAGAT GAAGCGGCCA CCACAATAAT GCCGGACAGC 
GAGTCGGTGC CAGTGATTAA CATTGTTGAA GGACAACACA TGCTGCAGCA GGAGGATCAG 
AAGGATGAGG AGGAGGAGGG GGTGGTCAAG GAGTCGGAGA GCAGTTCCAC CACCGAGGCG 
TCGACCACCA CCACCGAGCC ATCGCCATTT GTGGCCTTTG CTGGCGAGGG ACGATCGGCG 
GGTGGCGGCA ATGATATCGA GCTGTTTCTG CACCACAATG GCTCTACACA CGAGCAGCTC 
ATGGATCTTA GTGATGTCAG CATGGACGGA GATCAGAACG AGGGCAGCAG CAAAACAGAG 
AGCAGCACTA CTAGCACCAC CACGACCACT GCTCAGCCGG AAACGGAAAT GCCGAAAATT 
GTGGAGATCA CTGCCAGCGG GGATACCATG CAGCGGGAAT GCCTGGCCAA CAACAAGAGC 
TATAAGCACG GCGAGTTGAT GGAGCGGGAT TGCGACGAGC GTTGCACCTG CAACCGCGGC 
GACTGGATGT GCGAGCCAAG GTGCAGGGGA CTAAGTTATC CGCGCGGCAG CCAGCGCAGC 
ATGGCCAATC CCAACTGTCT GGAGAAGATG GTGGAGGAGG ACGAATGCTG TCGGGTGATG 
GAATGCAGTG AGCCGCAGCT GGAGCCCACG GTGGTAGCCA CAGAGGGTGC TGCACCTTCC 
ACCAATGGAA CGGGAGAATC GGCTGTGACC CTGCCCACGA CCGATGATGA AGCCACGCCC 
AAGCCCCGAA CTGATTGCCA CTACAACAGC GGTGTCTACA AATTCCGGGA GCGTCTGGAG 
ATCGGGTGCG AGCAGATCTG TCATTGTGCC GAAGGAGGCG TCATGGATTG CAGGCCACGC 
TGCCCGGAAC GGAATCACAC GCGTCTGGAC AAGTGTGTGT ATGTAAAGGA CCCGAAGGAC 
GTGTGCTGCC AACTGGAGCT CTGCGATGTC ACGCTGGACG ATCACGAACA GCAGCCAACG 
CCGCTGCAGA GCAACAACAA CGAAGATCCA GAGGAGATCG ACCCCTTCCG CTTCCAGGAG 
CAGGCTCGCG ACGCCGGAGG CGCCAAGCCC ACTTGCACAT TCAAGGGCGC AGAATATGAT 
GTGGGTCAGC AGTTCCGCGA TGGCTGCGAC CAGTTGTGCA TTTGCAATGA GCAGGGCATT 
CACTGTGCCA AGCTGGAGTG CCCCTCGAAC TTTGGCCTGG ATGTCCAGGA TCCGCACTGC 
ATACGCTGGG AACCGGTTCC GGCGGACTTC AAGCCCTCGC CGCCGAATTG CTGTCCGGAG 
AGCATGCGTT GCGTCGACAA TGGCACATGC AGTTACCAAG GCGTCCAGAT CGAGAACTGG 
TCCCCTGTCC CAGCCAACCT GACAGGTTGC GATCAGCATT GTTATTGCGA GAATGGACGG 
GTAGAGTGCA GGGCAGCTTG TCCTCCGGTT CCAGCTCTTC CTCCGGCGGA CTTGCCCTGC 
CATCCAGCCT TGGCCCGCCT GCTGCCCATT CCCGATGACG AGTGCTGCAA GCACTGGATG 
TGTGCCCCCC AAATCCCGAA AATCGGAGGT GCGGGTCAGG ACGAAGAGAC GGAAGCTACT 
TCAACCCATT CCTCGATTCC AGCAAATGAA ACAACAACAA CGACAGCGAC AGCAAATAAA 
TCGACCAGTA TACCTAGCAA AGTACCCCAA ATCAAGAAGG ACGAGGAGAA GAGACCACCA 
GCAAGTGGCG CCTTCTATCC AACCTTGGAT GGCAAGCCAC CCAAGTCGAT TGGTGGTCTT 
GGTATCTTCG AGAAGCCGGA AAAACCAGAG AAGGCCCACA AGAAAGTGCA ACATCAACAG 
CAGCAGCATC AGCAGCAGGA GCAGCAGGAG CAGCAGCAGC ACCAGAATGA TGTTATATTC 
GACGGTGATC GCACAGAGGA GCAGGAGGAG CCTTTGCCAC CGAACGGCGG TTTTGTGCCC 
TTCCAATTCG GCCAGCAGCA TCCGCATCAG CCACATCTTG GTCCGTATGG CTTCTACAAT 
CCCGTGAAGC CCGTTTACGA GGACTATAAT CCCTATGAGC CGTACGACAT CAATCCCAAT 
GGCACACCAC AGGGCAAACC GCCTCCAGTG CCCACTAGTC AGTCCGATTT GTTCAATATA 
TTAGGTGCGG AACAACCAGG ACACCCGGTT CATCCTGGCC ATGGTGGACC CCCCCGCATT 
CATCCTGGGC AAACGCAAAA AGACAATCAC AACCTGGGAC CACAAGTTAG AATCGAACAG 
ATACTGCAGC ACCTGCAGCA AACTGTTCCA GGTGGACCAC CACCACCTCC GCCCCACCAG 
CAACACCAAT CCCTGACACC GCAGCTGCAT CCGCAGCAGC AACAAATTTC GCAGCAACAT 
CCTGGTCATT ATGTGCCCAT CGTGCACAGT GGAGTGCCGC CACCGCCACC AGGACATGGC 
ATTGCCATTG TCGATGGGCA AACAGTGGCC TATGAGAGTT ATCCTGTGAT CCCGGGACTG 
GGAGTACCAC AGCACCATCC CCAGCAGCAC CAGACGACCC CGCAGCAACA CTTGCAGCAG 
ACAATCCTGC CTAGCTCGAG CACCACCTCC GGACTCTCGA CGCAGGCTAG TGAGCACAGT 
CTGCACCAGA ACCAGGGCAA GCTGGCCAAA CAGCAGCAGT CAGGAGCCAA CAACCTGCAA 
CCTGATATCG AAGTTCACAC ACTGGAGGCC ATCGATCCTC GTTCCATTCG CATCGTCTTC 
ACCGTTCCCC AGGTCTATGT GAACCTCCAT GGACGCGTGG AGCTGCGCTA CTCGAATGGA 
CCCAGTAACG ATACATCCAC CTGGGAACAA CAGATTTTTG CTCCGCCCGA GGACCTCATT 
GCCACATCCC AGATGGAGTT CGATCTGCCC AGTCTGGAAC CAAACTCACT GTACAAGGTG 
AAGATCACCT TAATCCTTCG TGATCTAAAC TCGCAGCCCA CGAGCAGTAT CTACACCGTG 
AAGACGCCAC CTGAAAGGAC CATCACTCCG CCACCTCCGT TCCCCGATTA CAGGCCAGAC 
TTCCAGGACA TCTTTAAGAA CGTTGAGGAT CCAGAACTGA CGGTCAGCGA AACGAATGCC 
AGCTGGTTGC AGTTGACATG GAAGAAACTT GGAGACGACC AAATGGAATA TGTGGACGGA 
GTTCAGCTGC GCTACAAGGA ACTGACGGGC ATGATCTACT CCTCAACGCC TCTGATCCAT 
CGCACGTTGA CCAGCTACAC CATCCAGAAC CTTCAGCCGG ATACGGGCTA CGAGATTGGG 
CTGTACTACA TCCCATTGGC TGGACATGGA GCTGAATTGC GTGCCGGACA CATGATTAAG 
GTGCGAACTG CCCAGAAGGT GGACGTGTAT GGCTTCGATG TGACCGTTAA CGTAACCAAG 
GTGAAGACCC AGAGTGTCGA GATCTCATGG AACGGAGTGC CCTATCCGGA GGACAAGTTC 
GTGCACATTT ATCGTGCCAT CTACCAGAGC GACGCTGGCA AGGAGGACTC CAGCGTCTTC 
AAGGTGGCCA AGCGGGACAG CACCACTGGT ACCCTGATCA TGGATCTCAA ACCAGGCACC 
AAGTACCGCC TCTGGCTGGA AATGTACCTG ACCAACGGCA ACACCAAGAA GAGCAACGTT 
GTCAACTTCA TCACGAAGCC AGGTGGTCCA GCCACTCCCG GAAAGACTGG AAAACTCCTA 
ACGGCGGGAA CGGACCAACC CGTAGGCGAT TACTACGGCC CCCTTGTGGT GGTTTCTGTG 
ATCGCCGCTC TGGCGATCAT GTCTACTTTG GCCCTGCTAC TTATTATCAC CAGGAGACGA 
GTTCATCAAA CGGCATCCAT TACGCCACCA CGAAAAAGCG ACGCTGCCTA CGATAATCCC 
TCATACAAGG TGGAGATCCA ACAGGAGACT ATGAATCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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sas cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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