gpp cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	gpp
Entrez Gene ID	40793
Full Name	grappa
Synonyms	0732/14,BcDNA:SD05230,CG10272,CG33324,CG42803,DOT1L,Dmel\CG42803,Dmel_CG10272,Dmel_CG33324,Gpp,anon-WO0118547.319,dDOT1L,dDot1,grp,l(3)03342,l(3)S073214b,l(3)sA3484
General protein information	Preferred Names grappa Names CG42803 gene product from transcript CG42803-RF CG42803-PB CG42803-PC CG42803-PD CG42803-PE CG42803-PF gpp-PB gpp-PC gpp-PD gpp-PE gpp-PF lethal (3) S073214b
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 83E7-83E7

mRNA and Protein(s)

mRNA	Protein	Name
NM_169143.2	NP_731084.1	grappa, isoform B
NM_141398.4	NP_649655.1	grappa, isoform E
NM_001275387.1	NP_001262316.1	grappa, isoform F
NM_169144.2	NP_731085.2	grappa, isoform D
NM_169142.2	NP_731083.1	grappa, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

gpp is required for normal lifespan and stress resistance, and its overexpression increases stress resistance in Drosophila, without obvious induction of representative anti-oxidant genes.
Title: Overexpression of grappa encoding a histone methyltransferase enhances stress resistance in Drosophila.

The following gpp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gpp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04082	NM_169143.2 Latest version!	Drosophila melanogaster grappa (gpp), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa04082	NM_169142.2 Latest version!	Drosophila melanogaster grappa (gpp), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa04082	NM_141398.4 Latest version!	Drosophila melanogaster grappa (gpp), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa04083	NM_169144.2 Latest version!	Drosophila melanogaster grappa (gpp), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OFa07560	NM_001275387.1 Latest version!	Drosophila melanogaster grappa (gpp), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gpp ( NM_169143.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731084.1 Drosophila melanogaster grappa (gpp), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa04082
Clone ID Related Accession (Same CDS sequence)	NM_169143.2 , NM_141398.4 , NM_169142.2
Accession Version	NM_169143.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	grappa, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 31, 2011 this sequence version replaced NM_169143.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACAC CCCAAGTCAA GGACTTGGTG TTGCGCTCGC CCGCTGGCTC CTCCGATGTC 
ATTTCCTTCG CCTGGCCCCT GCAGATCGGA CACGGACAGG ATAAGCACGA CAATGGCATC 
GACATCATCG ACACCATTAA GTTCGTCTGC GATGAACTGC CATCGATGTC ATCGGCCTTC 
GAGGAAACCA ATCTCCACCA AATTGACACT GCCTGCTATA AGACTATGAC CGGTCTGGTC 
GATCGCTTCA ACAAGGCCGT CGACAGTATC GTTGCATTGG AAAAAGGAAC TTCACTGCCC 
GCCGAGCGCC TGAACAAGTT CGCTCACCCT AGCCTTCTAA GACACATATT GCAATTAGTT 
TACAATGCCG CCGTACTCGA TCCGGATAAA CTCAACCAGT ACGAGCCCTT CTCGCCAGAG 
GTTTACGGCG AGACGAGCTA TGAGCTGGTG CAGCAGATGC TTAAGCACGT CACTGTCAGC 
AAGGAGGACA CGTTTATCGA TCTCGGCTCG GGGGTGGGCC AGGTGGTTCT GCAGATGGCC 
GGATCGTTTC CCCTGAAAAC CTGCATCGGC ATCGAGAAGG CCGACACGCC GGCGCGATAT 
GCGGAACGCA TGGATGTTAT CTTCCGCCAG TATATGGGAT GGTTTGGAAA ACGCTTTTGC 
GAATACAAGC TGATAAAGGG TGACTTCCTG GTCGATGAGC ATCGTGAAAA CATCACCTCA 
TCGACGCTGG TTTTTGTAAA TAACTTCGCC TTTGGGCCAA CTGTGGACCA TCAGCTCAAG 
GAACGGTTCG CAGATCTTCG CGACGGAGCA CGGGTCGTGT CCTCCAAGTC GTTTTGCCCG 
CTGAACTTTC GGATCACAGA CAGAAACCTC AGTGACATTG GCACCATTAT GCACGTCAGC 
GAAATTCCGC CCCTCAAGGG GTCCGTTTCC TGGACCTGCA AGCCCGTTTC GTATTATCTG 
CATGTGATCG ATCGCACCAT ATTGGAGCGA TACTTTCAGC GCCTGAAAAC TAAGGGCGGC 
AACGATCACG AGAGCGTGGG AACTGTTCGC ACCACACGTG ATCGCGCCAA GCGAGAGGCA 
AACGTTGGCC AACATCATCA CAACAATCAT CATAGCAACA ACCACGCCAA CAGCAACAAC 
CATCAGAGGG ATCGGGAGCA GTCGAACGGA GCTACCGCAA CTGCAGCTCA TCAGCAACGG 
CATCAGTCCC AGTCGCCAGC TAATGTAAGC GGTGCGGGAA TAGTACTTGC AGCTAGCGGG 
CAACAAGCAG CTTCCAAAAC ACGCCAGCAA CTGCAGCATC AGCATAACCA GCAGCAACGC 
AGCTTGGACA TGGAAAGCAG CACGGAAAGC GATGGCGACG CAACGAATGG CAATGGTGGC 
AATACCACCA CTGCCACCAA TACCACCTCC GCCTCCAATG GCCCGATGAC TAGGAAAGTT 
TGGTCCGACT GGTGCAGTTC CAAAGGCAAG AGTTCGCAGT CTGACGACGA GGAGAACAAC 
AACTCGAACA GCAATGGCGG CAGTAATGGT GGTAGCATTG GAGGAGGATC AGTTGGCCGA 
CAGGCTCGGG CTACAACCCA GAAGAAGCGC AAAAAGTTGA CTAGGAAGGC TGCGATAGCC 
AGCAAATCTG CTGCCGCTGC CCAAAGGGAA GCGGAGGCGG CAGCGGCGGC GGCGGTATCC 
GTACCAAGCA AGGAGTCCAG TTCCAAAGAG GATCCACCAA GGGCTGCTAG CGCCGGACCT 
GGTCGCAAAG GACGCATGAA GAAGGGTGCG CGCGGCAGGA AGTCTTTAAA GATTGTTGGA 
CTAGAAGCTC TGCACAAGCA AACAGTGTTG AGTACCTCCT TGGATACCAT GACCAAGAAG 
TTGCCTGCAG CTCCTGGAAC CGTGGATCAA CAGCTTACTG CTTTGCTCAC GGAAAACATG 
TCGCATGCAG AGTTGGATAT TCCAACGGCG CCACAAGACA CGCCTTATGC CTTGCAGATC 
CTTCTAGACG TGTTCCGTTC GCAGTACACA TCGATGATCG AGCACATGAA GTCAAGTGCC 
TATGTGCCTC AAGTTCAGAA GCAGATTGCC CAAGAGCAGG AGCGGATGGC CAGGCTGAAA 
AACAGAGCCA GCCAGTTGGA TAAGCAGATA AAAGTGCTGA TCGATGACAG TGTGGCTTTA 
CTTAAAGTGC GAATGAACGA GCTGGGCATC CATGTAAACT CACCAAACGA TTTGATCGCC 
CAGGCAAAGG AGATTGTGGG TAGACATAAG GATCTACAGC ACACTGCAAG TCGAATGCGA 
AACGAAGTAA CCTTCTATGA GGGCGAACAG AAGCTGCTCC TTAACAAACA ACTAAAAAAT 
CTACCCGAGT ATCAAAAACT CTGTGGTACT GTTAATGGAA AGGTCAAGCT GGAGGTGCCC 
CCGGAACTTT CCGAGACCAC GGCCCAGGAG CTGGTGCTAA AGGAGATCGC CAACACGCTA 
AGTCAACGAA AGAAACTTTA CGCCCAGGTG TCCACCATTG AACAGGAGAC TTCGGTTTTG 
CAGAAGACAG CAGAGGAAAG GTCCACGGCA GCGACGCTCT TGGCACAAGG AACAAATATG 
ATTGTGTCGA CCGGATCATC TTCCTCCTCC TCTACCACTG TTTGTGCAAG TGCAGTTACG 
GCGCAGAGCA ACAAGTTGAA CTCCGTGAAG AACTCTCGTC GCAATCGCGA GCACCGAGCG 
CGTTCCCAGG AGTGGCCAGA GGTTCCCGAA GTGGGTAAAA TCCAGGAAAG CAATCCCGAA 
GTGCTGGCAC AGAAAATAGT CGAGACCTGC CGCCAAATCG AAGCTGGCAA GTTCCAAGGA 
GCAGGTGCGC CCTCATCGCA AGTGAATGGC AAGAATAAAG CAATTATAGA GGTCCCACCA 
CCTCCGGCCA CTGCTCCAGT GAGCATTAAA TCCTCGCCTG GGCACCACTA TAAAGACACA 
ACACTGATGC CAGCACCCAA ACAACAACAG CAACAACAGA TGACGCTTTC CCAGCTTCCG 
AAGTGTGAGC TGCCCGGACT ATCGACTAGT CGCAAACAGG AATCTCCCAA GGTTGCCAAC 
TTTGAGGATC GGTTAAAGAG TATCATCACC ACGGCCTTAA ACGAAGATCA GGAGCAGCGC 
AGCAAGGCAG TGGAGTCGTC ACCCTCTCCC TCACCTCTTC ACAGTCCAGC ACCCAAGCGA 
TCAAAGCAGC ATCCGGCTGG AGCGATAAAC CCTGCCCAGT CGCTGCCCAA CAATCTGCAC 
AACATCATCA CCGTCTCCAC GCAGGGTTTG ATGCACCTTA ATGCCAATAC AACAATATCC 
CCAATAACAC CACCACTGCC GGGTCCAGGT GCAGGAGCAA CCGCATCCAC TGCGCCGCCT 
CCCCCAGCGA ATCTTCCTTA TGGGGCTTAC GGCGGGGCAG TGGCAAAGAC AACGATATCG 
GGGAAGTACC AGGCAGCTAA GGAACCGAAG TATTCGCCAG TTAGACAGGC ACCATTACCT 
CCTCCCCCCT CCCACATGGC ATCTTTGTAT CCAGCTGGAC AGCAGACTAC TCCGGCGGAC 
CTAGGTTACC AGCGGCGACG CAGCTCTGTT AGTGCCACTA GCTACGAACA CTATATGGTA 
CAGCAGCAGC AGCAACTCCA GCAACAACAA CTCATGCTAG CAGCCGCTGC TCATGCGGCC 
CAGCGTCAAC AGATGCGCGT AGAAGAACAA CAGCAGCAGC AGCAGCACCA ACACCACCAC 
CACCACCACC ACCACCATCC CCAACACCGT CTGCCGCAGC ATGTGCAGCA TCAGCATCCG 
CATCAGCATC ATCCCAATGA GTTCAAGGCA CCGCCGGCTG ATAGTCATCT CCAGCGATCG 
AGCAGTCGTG AGCAATTAAT AGTGGAGCCC CCGCAGACGC AACCGCTGGA GCTGCTGCCT 
CGAGCAAGTT CCGCCAATTC GGATTACAGC GGATATCGCA TCCGTCCGCC AAGCAGGCCA 
AGCTCCAACT CCTCGCAACC AGATTACACG CAAGTTTCAC CCGCAAAGAT GGCTCTGCGG 
CGGCATCTGT CGCAGGAGAA GCTGAGCCAA CATGTCACGC CCCAGGCCAC ACCGCCGCTT 
CCTGGTCACG GAGGAGCACC CACATCAGGG AAGACCATTG GCGATTTGGT TAACGGGGAA 
ATCGAGCGAA CGCTTGAGAT TTCGCATCAG AGCATCATTA ATGCGGCTGT GAATATGAGC 
ACTAGTGGCG CTAGCTTCAT GGAGCGGGCC TTCCTTAATG AGCGCTCCAA CGATCGTTTG 
CTGATCAACT TGAATGCCCA ACGACCAGAG AGAGTTCATG TTCGTCCGCT CTCCGAAGAA 
TCGCAGGATC CACAGCCCAC CAGTTATGCT CAGGAGAGAG GACCTGGTCT AGGTGCTGGT 
GGAGCAGCAG CTGGAGGTAA CAGCAATCTG GCCACTTTGG CACATGTAGC CTACGCCCAA 
AAGGCACAGG GCGGCGCTCG AGCCAACGCA GGAACGGCAC CACCCGCAAC CCACTCCTCC 
TCTGCTCGTT CTGGACGTGA CTATCAGCCT GTGGCTTTGC CCCGTGCTGA GCTTAAGGGC 
AGCATTGAGG CCTATTTCCA CGAGGAGCAG CAGCAGAAGC AGTCGAAGGG CGCAGGATCA 
GCAGGATCGT CCTCTTTGCG GGGACCGCGT CTTAATGGTG CCAATCCTCC ATTGGAAGGT 
CTAGCAGCAT CGCTGCAGGA TCATGTCCGT GCCAGAAAGT ACAAGGAGGA GACCGAGGAG 
CGTCAACGAC GTGCAGCAGC CGCTGCCTCG TCTTCGGCGG GACCGCCTGC TGGCATGGAA 
CTGCCAACGC ACTATGCTCA TCAGGCGCCT CCAGCCCACA GCTACCACCA CCATGGTGCA 
AGCATCAATG GAACACCGCA CAAAGTAGAA CTGGGAATAA AACGCAGCTC ACCGCTGGCG 
CCGCATCAGC AACCGCCACG TCCCTCGAAA TTGGCACACT ACGAGCCGCC AACGACGCAG 
CAGCAGCATG CCCATGCCCA CCTGTACGCC AACGGACAAG TGCTACCTCC GCCTCCAGCT 
CATGATGCGA CCACGCCCTC CCCGACGCCC TCGTCATCTT CATCGTCCTG CGGACGTCGA 
AGCAACAGCA ACAATGGAAA GTTGCTCGTG GATCCACCTT TGCTAATGAG TCCCGAGATC 
AATTCACTGT TGGGCGACGA AAGGCCTTTG CAGCTGTCGC ACCACCAACA GCAGCAGCAG 
CAAATGCTGC ATCACCATCA ATCGCAGCAA CAGCAGCATC TGCAGCTGAC CCAACAGCAA 
CTGCGTGTAG CTCATCTTGG ACATGGTCTT AGTCACGGTC ACAGCACCAT GCCCACTTTG 
GGCGGACAAC GCAATGGCAA TGGCAATGCC GCCGACGATG TTAACGATTT GGCAACGCAA 
CGTACTATAA CTAATTACGA TCCCAGACGT CGCCTTCGCA CGACTTTGTC TGGCCCAACG 
AAACTATCAG CTGCCCATAG TAATCAAAAT CTGAACGGAT ATGTTATGGC CGATAGTAGT 
AGTAGTTGCC CCACAATACC CCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731084.1
CDS	1464..7010
Translation	MATPQVKDLVLRSPAGSSDVISFAWPLQIGHGQDKHDNGIDIIDTIKFVCDELPSMSSAFEETNLHQIDTACYKTMTGLVDRFNKAVDSIVALEKGTSLPAERLNKFAHPSLLRHILQLVYNAAVLDPDKLNQYEPFSPEVYGETSYELVQQMLKHVTVSKEDTFIDLGSGVGQVVLQMAGSFPLKTCIGIEKADTPARYAERMDVIFRQYMGWFGKRFCEYKLIKGDFLVDEHRENITSSTLVFVNNFAFGPTVDHQLKERFADLRDGARVVSSKSFCPLNFRITDRNLSDIGTIMHVSEIPPLKGSVSWTCKPVSYYLHVIDRTILERYFQRLKTKGGNDHESVGTVRTTRDRAKREANVGQHHHNNHHSNNHANSNNHQRDREQSNGATATAAHQQRHQSQSPANVSGAGIVLAASGQQAASKTRQQLQHQHNQQQRSLDMESSTESDGDATNGNGGNTTTATNTTSASNGPMTRKVWSDWCSSKGKSSQSDDEENNNSNSNGGSNGGSIGGGSVGRQARATTQKKRKKLTRKAAIASKSAAAAQREAEAAAAAAVSVPSKESSSKEDPPRAASAGPGRKGRMKKGARGRKSLKIVGLEALHKQTVLSTSLDTMTKKLPAAPGTVDQQLTALLTENMSHAELDIPTAPQDTPYALQILLDVFRSQYTSMIEHMKSSAYVPQVQKQIAQEQERMARLKNRASQLDKQIKVLIDDSVALLKVRMNELGIHVNSPNDLIAQAKEIVGRHKDLQHTASRMRNEVTFYEGEQKLLLNKQLKNLPEYQKLCGTVNGKVKLEVPPELSETTAQELVLKEIANTLSQRKKLYAQVSTIEQETSVLQKTAEERSTAATLLAQGTNMIVSTGSSSSSSTTVCASAVTAQSNKLNSVKNSRRNREHRARSQEWPEVPEVGKIQESNPEVLAQKIVETCRQIEAGKFQGAGAPSSQVNGKNKAIIEVPPPPATAPVSIKSSPGHHYKDTTLMPAPKQQQQQQMTLSQLPKCELPGLSTSRKQESPKVANFEDRLKSIITTALNEDQEQRSKAVESSPSPSPLHSPAPKRSKQHPAGAINPAQSLPNNLHNIITVSTQGLMHLNANTTISPITPPLPGPGAGATASTAPPPPANLPYGAYGGAVAKTTISGKYQAAKEPKYSPVRQAPLPPPPSHMASLYPAGQQTTPADLGYQRRRSSVSATSYEHYMVQQQQQLQQQQLMLAAAAHAAQRQQMRVEEQQQQQQHQHHHHHHHHHPQHRLPQHVQHQHPHQHHPNEFKAPPADSHLQRSSSREQLIVEPPQTQPLELLPRASSANSDYSGYRIRPPSRPSSNSSQPDYTQVSPAKMALRRHLSQEKLSQHVTPQATPPLPGHGGAPTSGKTIGDLVNGEIERTLEISHQSIINAAVNMSTSGASFMERAFLNERSNDRLLINLNAQRPERVHVRPLSEESQDPQPTSYAQERGPGLGAGGAAAGGNSNLATLAHVAYAQKAQGGARANAGTAPPATHSSSARSGRDYQPVALPRAELKGSIEAYFHEEQQQKQSKGAGSAGSSSLRGPRLNGANPPLEGLAASLQDHVRARKYKEETEERQRRAAAAASSSAGPPAGMELPTHYAHQAPPAHSYHHHGASINGTPHKVELGIKRSSPLAPHQQPPRPSKLAHYEPPTTQQQHAHAHLYANGQVLPPPPAHDATTPSPTPSSSSSSCGRRSNSNNGKLLVDPPLLMSPEINSLLGDERPLQLSHHQQQQQQMLHHHQSQQQQHLQLTQQQLRVAHLGHGLSHGHSTMPTLGGQRNGNGNAADDVNDLATQRTITNYDPRRRLRTTLSGPTKLSAAHSNQNLNGYVMADSSSSCPTIPQ

Target ORF information:

RefSeq Version	NM_169143.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster grappa (gpp), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169143.2

ORF Insert Sequence:

ATGGCCACAC CCCAAGTCAA GGACTTGGTG TTGCGCTCGC CCGCTGGCTC CTCCGATGTC 
ATTTCCTTCG CCTGGCCCCT GCAGATCGGA CACGGACAGG ATAAGCACGA CAATGGCATC 
GACATCATCG ACACCATTAA GTTCGTCTGC GATGAACTGC CATCGATGTC ATCGGCCTTC 
GAGGAAACCA ATCTCCACCA AATTGACACT GCCTGCTATA AGACTATGAC CGGTCTGGTC 
GATCGCTTCA ACAAGGCCGT CGACAGTATC GTTGCATTGG AAAAAGGAAC TTCACTGCCC 
GCCGAGCGCC TGAACAAGTT CGCTCACCCT AGCCTTCTAA GACACATATT GCAATTAGTT 
TACAATGCCG CCGTACTCGA TCCGGATAAA CTCAACCAGT ACGAGCCCTT CTCGCCAGAG 
GTTTACGGCG AGACGAGCTA TGAGCTGGTG CAGCAGATGC TTAAGCACGT CACTGTCAGC 
AAGGAGGACA CGTTTATCGA TCTCGGCTCG GGGGTGGGCC AGGTGGTTCT GCAGATGGCC 
GGATCGTTTC CCCTGAAAAC CTGCATCGGC ATCGAGAAGG CCGACACGCC GGCGCGATAT 
GCGGAACGCA TGGATGTTAT CTTCCGCCAG TATATGGGAT GGTTTGGAAA ACGCTTTTGC 
GAATACAAGC TGATAAAGGG TGACTTCCTG GTCGATGAGC ATCGTGAAAA CATCACCTCA 
TCGACGCTGG TTTTTGTAAA TAACTTCGCC TTTGGGCCAA CTGTGGACCA TCAGCTCAAG 
GAACGGTTCG CAGATCTTCG CGACGGAGCA CGGGTCGTGT CCTCCAAGTC GTTTTGCCCG 
CTGAACTTTC GGATCACAGA CAGAAACCTC AGTGACATTG GCACCATTAT GCACGTCAGC 
GAAATTCCGC CCCTCAAGGG GTCCGTTTCC TGGACCTGCA AGCCCGTTTC GTATTATCTG 
CATGTGATCG ATCGCACCAT ATTGGAGCGA TACTTTCAGC GCCTGAAAAC TAAGGGCGGC 
AACGATCACG AGAGCGTGGG AACTGTTCGC ACCACACGTG ATCGCGCCAA GCGAGAGGCA 
AACGTTGGCC AACATCATCA CAACAATCAT CATAGCAACA ACCACGCCAA CAGCAACAAC 
CATCAGAGGG ATCGGGAGCA GTCGAACGGA GCTACCGCAA CTGCAGCTCA TCAGCAACGG 
CATCAGTCCC AGTCGCCAGC TAATGTAAGC GGTGCGGGAA TAGTACTTGC AGCTAGCGGG 
CAACAAGCAG CTTCCAAAAC ACGCCAGCAA CTGCAGCATC AGCATAACCA GCAGCAACGC 
AGCTTGGACA TGGAAAGCAG CACGGAAAGC GATGGCGACG CAACGAATGG CAATGGTGGC 
AATACCACCA CTGCCACCAA TACCACCTCC GCCTCCAATG GCCCGATGAC TAGGAAAGTT 
TGGTCCGACT GGTGCAGTTC CAAAGGCAAG AGTTCGCAGT CTGACGACGA GGAGAACAAC 
AACTCGAACA GCAATGGCGG CAGTAATGGT GGTAGCATTG GAGGAGGATC AGTTGGCCGA 
CAGGCTCGGG CTACAACCCA GAAGAAGCGC AAAAAGTTGA CTAGGAAGGC TGCGATAGCC 
AGCAAATCTG CTGCCGCTGC CCAAAGGGAA GCGGAGGCGG CAGCGGCGGC GGCGGTATCC 
GTACCAAGCA AGGAGTCCAG TTCCAAAGAG GATCCACCAA GGGCTGCTAG CGCCGGACCT 
GGTCGCAAAG GACGCATGAA GAAGGGTGCG CGCGGCAGGA AGTCTTTAAA GATTGTTGGA 
CTAGAAGCTC TGCACAAGCA AACAGTGTTG AGTACCTCCT TGGATACCAT GACCAAGAAG 
TTGCCTGCAG CTCCTGGAAC CGTGGATCAA CAGCTTACTG CTTTGCTCAC GGAAAACATG 
TCGCATGCAG AGTTGGATAT TCCAACGGCG CCACAAGACA CGCCTTATGC CTTGCAGATC 
CTTCTAGACG TGTTCCGTTC GCAGTACACA TCGATGATCG AGCACATGAA GTCAAGTGCC 
TATGTGCCTC AAGTTCAGAA GCAGATTGCC CAAGAGCAGG AGCGGATGGC CAGGCTGAAA 
AACAGAGCCA GCCAGTTGGA TAAGCAGATA AAAGTGCTGA TCGATGACAG TGTGGCTTTA 
CTTAAAGTGC GAATGAACGA GCTGGGCATC CATGTAAACT CACCAAACGA TTTGATCGCC 
CAGGCAAAGG AGATTGTGGG TAGACATAAG GATCTACAGC ACACTGCAAG TCGAATGCGA 
AACGAAGTAA CCTTCTATGA GGGCGAACAG AAGCTGCTCC TTAACAAACA ACTAAAAAAT 
CTACCCGAGT ATCAAAAACT CTGTGGTACT GTTAATGGAA AGGTCAAGCT GGAGGTGCCC 
CCGGAACTTT CCGAGACCAC GGCCCAGGAG CTGGTGCTAA AGGAGATCGC CAACACGCTA 
AGTCAACGAA AGAAACTTTA CGCCCAGGTG TCCACCATTG AACAGGAGAC TTCGGTTTTG 
CAGAAGACAG CAGAGGAAAG GTCCACGGCA GCGACGCTCT TGGCACAAGG AACAAATATG 
ATTGTGTCGA CCGGATCATC TTCCTCCTCC TCTACCACTG TTTGTGCAAG TGCAGTTACG 
GCGCAGAGCA ACAAGTTGAA CTCCGTGAAG AACTCTCGTC GCAATCGCGA GCACCGAGCG 
CGTTCCCAGG AGTGGCCAGA GGTTCCCGAA GTGGGTAAAA TCCAGGAAAG CAATCCCGAA 
GTGCTGGCAC AGAAAATAGT CGAGACCTGC CGCCAAATCG AAGCTGGCAA GTTCCAAGGA 
GCAGGTGCGC CCTCATCGCA AGTGAATGGC AAGAATAAAG CAATTATAGA GGTCCCACCA 
CCTCCGGCCA CTGCTCCAGT GAGCATTAAA TCCTCGCCTG GGCACCACTA TAAAGACACA 
ACACTGATGC CAGCACCCAA ACAACAACAG CAACAACAGA TGACGCTTTC CCAGCTTCCG 
AAGTGTGAGC TGCCCGGACT ATCGACTAGT CGCAAACAGG AATCTCCCAA GGTTGCCAAC 
TTTGAGGATC GGTTAAAGAG TATCATCACC ACGGCCTTAA ACGAAGATCA GGAGCAGCGC 
AGCAAGGCAG TGGAGTCGTC ACCCTCTCCC TCACCTCTTC ACAGTCCAGC ACCCAAGCGA 
TCAAAGCAGC ATCCGGCTGG AGCGATAAAC CCTGCCCAGT CGCTGCCCAA CAATCTGCAC 
AACATCATCA CCGTCTCCAC GCAGGGTTTG ATGCACCTTA ATGCCAATAC AACAATATCC 
CCAATAACAC CACCACTGCC GGGTCCAGGT GCAGGAGCAA CCGCATCCAC TGCGCCGCCT 
CCCCCAGCGA ATCTTCCTTA TGGGGCTTAC GGCGGGGCAG TGGCAAAGAC AACGATATCG 
GGGAAGTACC AGGCAGCTAA GGAACCGAAG TATTCGCCAG TTAGACAGGC ACCATTACCT 
CCTCCCCCCT CCCACATGGC ATCTTTGTAT CCAGCTGGAC AGCAGACTAC TCCGGCGGAC 
CTAGGTTACC AGCGGCGACG CAGCTCTGTT AGTGCCACTA GCTACGAACA CTATATGGTA 
CAGCAGCAGC AGCAACTCCA GCAACAACAA CTCATGCTAG CAGCCGCTGC TCATGCGGCC 
CAGCGTCAAC AGATGCGCGT AGAAGAACAA CAGCAGCAGC AGCAGCACCA ACACCACCAC 
CACCACCACC ACCACCATCC CCAACACCGT CTGCCGCAGC ATGTGCAGCA TCAGCATCCG 
CATCAGCATC ATCCCAATGA GTTCAAGGCA CCGCCGGCTG ATAGTCATCT CCAGCGATCG 
AGCAGTCGTG AGCAATTAAT AGTGGAGCCC CCGCAGACGC AACCGCTGGA GCTGCTGCCT 
CGAGCAAGTT CCGCCAATTC GGATTACAGC GGATATCGCA TCCGTCCGCC AAGCAGGCCA 
AGCTCCAACT CCTCGCAACC AGATTACACG CAAGTTTCAC CCGCAAAGAT GGCTCTGCGG 
CGGCATCTGT CGCAGGAGAA GCTGAGCCAA CATGTCACGC CCCAGGCCAC ACCGCCGCTT 
CCTGGTCACG GAGGAGCACC CACATCAGGG AAGACCATTG GCGATTTGGT TAACGGGGAA 
ATCGAGCGAA CGCTTGAGAT TTCGCATCAG AGCATCATTA ATGCGGCTGT GAATATGAGC 
ACTAGTGGCG CTAGCTTCAT GGAGCGGGCC TTCCTTAATG AGCGCTCCAA CGATCGTTTG 
CTGATCAACT TGAATGCCCA ACGACCAGAG AGAGTTCATG TTCGTCCGCT CTCCGAAGAA 
TCGCAGGATC CACAGCCCAC CAGTTATGCT CAGGAGAGAG GACCTGGTCT AGGTGCTGGT 
GGAGCAGCAG CTGGAGGTAA CAGCAATCTG GCCACTTTGG CACATGTAGC CTACGCCCAA 
AAGGCACAGG GCGGCGCTCG AGCCAACGCA GGAACGGCAC CACCCGCAAC CCACTCCTCC 
TCTGCTCGTT CTGGACGTGA CTATCAGCCT GTGGCTTTGC CCCGTGCTGA GCTTAAGGGC 
AGCATTGAGG CCTATTTCCA CGAGGAGCAG CAGCAGAAGC AGTCGAAGGG CGCAGGATCA 
GCAGGATCGT CCTCTTTGCG GGGACCGCGT CTTAATGGTG CCAATCCTCC ATTGGAAGGT 
CTAGCAGCAT CGCTGCAGGA TCATGTCCGT GCCAGAAAGT ACAAGGAGGA GACCGAGGAG 
CGTCAACGAC GTGCAGCAGC CGCTGCCTCG TCTTCGGCGG GACCGCCTGC TGGCATGGAA 
CTGCCAACGC ACTATGCTCA TCAGGCGCCT CCAGCCCACA GCTACCACCA CCATGGTGCA 
AGCATCAATG GAACACCGCA CAAAGTAGAA CTGGGAATAA AACGCAGCTC ACCGCTGGCG 
CCGCATCAGC AACCGCCACG TCCCTCGAAA TTGGCACACT ACGAGCCGCC AACGACGCAG 
CAGCAGCATG CCCATGCCCA CCTGTACGCC AACGGACAAG TGCTACCTCC GCCTCCAGCT 
CATGATGCGA CCACGCCCTC CCCGACGCCC TCGTCATCTT CATCGTCCTG CGGACGTCGA 
AGCAACAGCA ACAATGGAAA GTTGCTCGTG GATCCACCTT TGCTAATGAG TCCCGAGATC 
AATTCACTGT TGGGCGACGA AAGGCCTTTG CAGCTGTCGC ACCACCAACA GCAGCAGCAG 
CAAATGCTGC ATCACCATCA ATCGCAGCAA CAGCAGCATC TGCAGCTGAC CCAACAGCAA 
CTGCGTGTAG CTCATCTTGG ACATGGTCTT AGTCACGGTC ACAGCACCAT GCCCACTTTG 
GGCGGACAAC GCAATGGCAA TGGCAATGCC GCCGACGATG TTAACGATTT GGCAACGCAA 
CGTACTATAA CTAATTACGA TCCCAGACGT CGCCTTCGCA CGACTTTGTC TGGCCCAACG 
AAACTATCAG CTGCCCATAG TAATCAAAAT CTGAACGGAT ATGTTATGGC CGATAGTAGT 
AGTAGTTGCC CCACAATACC CCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gpp ( NM_141398.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649655.1 Drosophila melanogaster grappa (gpp), transcript variant E, mRNA.

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CloneID	OFa04082
Clone ID Related Accession (Same CDS sequence)	NM_169143.2 , NM_141398.4 , NM_169142.2
Accession Version	NM_141398.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	grappa, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_141398.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACAC CCCAAGTCAA GGACTTGGTG TTGCGCTCGC CCGCTGGCTC CTCCGATGTC 
ATTTCCTTCG CCTGGCCCCT GCAGATCGGA CACGGACAGG ATAAGCACGA CAATGGCATC 
GACATCATCG ACACCATTAA GTTCGTCTGC GATGAACTGC CATCGATGTC ATCGGCCTTC 
GAGGAAACCA ATCTCCACCA AATTGACACT GCCTGCTATA AGACTATGAC CGGTCTGGTC 
GATCGCTTCA ACAAGGCCGT CGACAGTATC GTTGCATTGG AAAAAGGAAC TTCACTGCCC 
GCCGAGCGCC TGAACAAGTT CGCTCACCCT AGCCTTCTAA GACACATATT GCAATTAGTT 
TACAATGCCG CCGTACTCGA TCCGGATAAA CTCAACCAGT ACGAGCCCTT CTCGCCAGAG 
GTTTACGGCG AGACGAGCTA TGAGCTGGTG CAGCAGATGC TTAAGCACGT CACTGTCAGC 
AAGGAGGACA CGTTTATCGA TCTCGGCTCG GGGGTGGGCC AGGTGGTTCT GCAGATGGCC 
GGATCGTTTC CCCTGAAAAC CTGCATCGGC ATCGAGAAGG CCGACACGCC GGCGCGATAT 
GCGGAACGCA TGGATGTTAT CTTCCGCCAG TATATGGGAT GGTTTGGAAA ACGCTTTTGC 
GAATACAAGC TGATAAAGGG TGACTTCCTG GTCGATGAGC ATCGTGAAAA CATCACCTCA 
TCGACGCTGG TTTTTGTAAA TAACTTCGCC TTTGGGCCAA CTGTGGACCA TCAGCTCAAG 
GAACGGTTCG CAGATCTTCG CGACGGAGCA CGGGTCGTGT CCTCCAAGTC GTTTTGCCCG 
CTGAACTTTC GGATCACAGA CAGAAACCTC AGTGACATTG GCACCATTAT GCACGTCAGC 
GAAATTCCGC CCCTCAAGGG GTCCGTTTCC TGGACCTGCA AGCCCGTTTC GTATTATCTG 
CATGTGATCG ATCGCACCAT ATTGGAGCGA TACTTTCAGC GCCTGAAAAC TAAGGGCGGC 
AACGATCACG AGAGCGTGGG AACTGTTCGC ACCACACGTG ATCGCGCCAA GCGAGAGGCA 
AACGTTGGCC AACATCATCA CAACAATCAT CATAGCAACA ACCACGCCAA CAGCAACAAC 
CATCAGAGGG ATCGGGAGCA GTCGAACGGA GCTACCGCAA CTGCAGCTCA TCAGCAACGG 
CATCAGTCCC AGTCGCCAGC TAATGTAAGC GGTGCGGGAA TAGTACTTGC AGCTAGCGGG 
CAACAAGCAG CTTCCAAAAC ACGCCAGCAA CTGCAGCATC AGCATAACCA GCAGCAACGC 
AGCTTGGACA TGGAAAGCAG CACGGAAAGC GATGGCGACG CAACGAATGG CAATGGTGGC 
AATACCACCA CTGCCACCAA TACCACCTCC GCCTCCAATG GCCCGATGAC TAGGAAAGTT 
TGGTCCGACT GGTGCAGTTC CAAAGGCAAG AGTTCGCAGT CTGACGACGA GGAGAACAAC 
AACTCGAACA GCAATGGCGG CAGTAATGGT GGTAGCATTG GAGGAGGATC AGTTGGCCGA 
CAGGCTCGGG CTACAACCCA GAAGAAGCGC AAAAAGTTGA CTAGGAAGGC TGCGATAGCC 
AGCAAATCTG CTGCCGCTGC CCAAAGGGAA GCGGAGGCGG CAGCGGCGGC GGCGGTATCC 
GTACCAAGCA AGGAGTCCAG TTCCAAAGAG GATCCACCAA GGGCTGCTAG CGCCGGACCT 
GGTCGCAAAG GACGCATGAA GAAGGGTGCG CGCGGCAGGA AGTCTTTAAA GATTGTTGGA 
CTAGAAGCTC TGCACAAGCA AACAGTGTTG AGTACCTCCT TGGATACCAT GACCAAGAAG 
TTGCCTGCAG CTCCTGGAAC CGTGGATCAA CAGCTTACTG CTTTGCTCAC GGAAAACATG 
TCGCATGCAG AGTTGGATAT TCCAACGGCG CCACAAGACA CGCCTTATGC CTTGCAGATC 
CTTCTAGACG TGTTCCGTTC GCAGTACACA TCGATGATCG AGCACATGAA GTCAAGTGCC 
TATGTGCCTC AAGTTCAGAA GCAGATTGCC CAAGAGCAGG AGCGGATGGC CAGGCTGAAA 
AACAGAGCCA GCCAGTTGGA TAAGCAGATA AAAGTGCTGA TCGATGACAG TGTGGCTTTA 
CTTAAAGTGC GAATGAACGA GCTGGGCATC CATGTAAACT CACCAAACGA TTTGATCGCC 
CAGGCAAAGG AGATTGTGGG TAGACATAAG GATCTACAGC ACACTGCAAG TCGAATGCGA 
AACGAAGTAA CCTTCTATGA GGGCGAACAG AAGCTGCTCC TTAACAAACA ACTAAAAAAT 
CTACCCGAGT ATCAAAAACT CTGTGGTACT GTTAATGGAA AGGTCAAGCT GGAGGTGCCC 
CCGGAACTTT CCGAGACCAC GGCCCAGGAG CTGGTGCTAA AGGAGATCGC CAACACGCTA 
AGTCAACGAA AGAAACTTTA CGCCCAGGTG TCCACCATTG AACAGGAGAC TTCGGTTTTG 
CAGAAGACAG CAGAGGAAAG GTCCACGGCA GCGACGCTCT TGGCACAAGG AACAAATATG 
ATTGTGTCGA CCGGATCATC TTCCTCCTCC TCTACCACTG TTTGTGCAAG TGCAGTTACG 
GCGCAGAGCA ACAAGTTGAA CTCCGTGAAG AACTCTCGTC GCAATCGCGA GCACCGAGCG 
CGTTCCCAGG AGTGGCCAGA GGTTCCCGAA GTGGGTAAAA TCCAGGAAAG CAATCCCGAA 
GTGCTGGCAC AGAAAATAGT CGAGACCTGC CGCCAAATCG AAGCTGGCAA GTTCCAAGGA 
GCAGGTGCGC CCTCATCGCA AGTGAATGGC AAGAATAAAG CAATTATAGA GGTCCCACCA 
CCTCCGGCCA CTGCTCCAGT GAGCATTAAA TCCTCGCCTG GGCACCACTA TAAAGACACA 
ACACTGATGC CAGCACCCAA ACAACAACAG CAACAACAGA TGACGCTTTC CCAGCTTCCG 
AAGTGTGAGC TGCCCGGACT ATCGACTAGT CGCAAACAGG AATCTCCCAA GGTTGCCAAC 
TTTGAGGATC GGTTAAAGAG TATCATCACC ACGGCCTTAA ACGAAGATCA GGAGCAGCGC 
AGCAAGGCAG TGGAGTCGTC ACCCTCTCCC TCACCTCTTC ACAGTCCAGC ACCCAAGCGA 
TCAAAGCAGC ATCCGGCTGG AGCGATAAAC CCTGCCCAGT CGCTGCCCAA CAATCTGCAC 
AACATCATCA CCGTCTCCAC GCAGGGTTTG ATGCACCTTA ATGCCAATAC AACAATATCC 
CCAATAACAC CACCACTGCC GGGTCCAGGT GCAGGAGCAA CCGCATCCAC TGCGCCGCCT 
CCCCCAGCGA ATCTTCCTTA TGGGGCTTAC GGCGGGGCAG TGGCAAAGAC AACGATATCG 
GGGAAGTACC AGGCAGCTAA GGAACCGAAG TATTCGCCAG TTAGACAGGC ACCATTACCT 
CCTCCCCCCT CCCACATGGC ATCTTTGTAT CCAGCTGGAC AGCAGACTAC TCCGGCGGAC 
CTAGGTTACC AGCGGCGACG CAGCTCTGTT AGTGCCACTA GCTACGAACA CTATATGGTA 
CAGCAGCAGC AGCAACTCCA GCAACAACAA CTCATGCTAG CAGCCGCTGC TCATGCGGCC 
CAGCGTCAAC AGATGCGCGT AGAAGAACAA CAGCAGCAGC AGCAGCACCA ACACCACCAC 
CACCACCACC ACCACCATCC CCAACACCGT CTGCCGCAGC ATGTGCAGCA TCAGCATCCG 
CATCAGCATC ATCCCAATGA GTTCAAGGCA CCGCCGGCTG ATAGTCATCT CCAGCGATCG 
AGCAGTCGTG AGCAATTAAT AGTGGAGCCC CCGCAGACGC AACCGCTGGA GCTGCTGCCT 
CGAGCAAGTT CCGCCAATTC GGATTACAGC GGATATCGCA TCCGTCCGCC AAGCAGGCCA 
AGCTCCAACT CCTCGCAACC AGATTACACG CAAGTTTCAC CCGCAAAGAT GGCTCTGCGG 
CGGCATCTGT CGCAGGAGAA GCTGAGCCAA CATGTCACGC CCCAGGCCAC ACCGCCGCTT 
CCTGGTCACG GAGGAGCACC CACATCAGGG AAGACCATTG GCGATTTGGT TAACGGGGAA 
ATCGAGCGAA CGCTTGAGAT TTCGCATCAG AGCATCATTA ATGCGGCTGT GAATATGAGC 
ACTAGTGGCG CTAGCTTCAT GGAGCGGGCC TTCCTTAATG AGCGCTCCAA CGATCGTTTG 
CTGATCAACT TGAATGCCCA ACGACCAGAG AGAGTTCATG TTCGTCCGCT CTCCGAAGAA 
TCGCAGGATC CACAGCCCAC CAGTTATGCT CAGGAGAGAG GACCTGGTCT AGGTGCTGGT 
GGAGCAGCAG CTGGAGGTAA CAGCAATCTG GCCACTTTGG CACATGTAGC CTACGCCCAA 
AAGGCACAGG GCGGCGCTCG AGCCAACGCA GGAACGGCAC CACCCGCAAC CCACTCCTCC 
TCTGCTCGTT CTGGACGTGA CTATCAGCCT GTGGCTTTGC CCCGTGCTGA GCTTAAGGGC 
AGCATTGAGG CCTATTTCCA CGAGGAGCAG CAGCAGAAGC AGTCGAAGGG CGCAGGATCA 
GCAGGATCGT CCTCTTTGCG GGGACCGCGT CTTAATGGTG CCAATCCTCC ATTGGAAGGT 
CTAGCAGCAT CGCTGCAGGA TCATGTCCGT GCCAGAAAGT ACAAGGAGGA GACCGAGGAG 
CGTCAACGAC GTGCAGCAGC CGCTGCCTCG TCTTCGGCGG GACCGCCTGC TGGCATGGAA 
CTGCCAACGC ACTATGCTCA TCAGGCGCCT CCAGCCCACA GCTACCACCA CCATGGTGCA 
AGCATCAATG GAACACCGCA CAAAGTAGAA CTGGGAATAA AACGCAGCTC ACCGCTGGCG 
CCGCATCAGC AACCGCCACG TCCCTCGAAA TTGGCACACT ACGAGCCGCC AACGACGCAG 
CAGCAGCATG CCCATGCCCA CCTGTACGCC AACGGACAAG TGCTACCTCC GCCTCCAGCT 
CATGATGCGA CCACGCCCTC CCCGACGCCC TCGTCATCTT CATCGTCCTG CGGACGTCGA 
AGCAACAGCA ACAATGGAAA GTTGCTCGTG GATCCACCTT TGCTAATGAG TCCCGAGATC 
AATTCACTGT TGGGCGACGA AAGGCCTTTG CAGCTGTCGC ACCACCAACA GCAGCAGCAG 
CAAATGCTGC ATCACCATCA ATCGCAGCAA CAGCAGCATC TGCAGCTGAC CCAACAGCAA 
CTGCGTGTAG CTCATCTTGG ACATGGTCTT AGTCACGGTC ACAGCACCAT GCCCACTTTG 
GGCGGACAAC GCAATGGCAA TGGCAATGCC GCCGACGATG TTAACGATTT GGCAACGCAA 
CGTACTATAA CTAATTACGA TCCCAGACGT CGCCTTCGCA CGACTTTGTC TGGCCCAACG 
AAACTATCAG CTGCCCATAG TAATCAAAAT CTGAACGGAT ATGTTATGGC CGATAGTAGT 
AGTAGTTGCC CCACAATACC CCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649655.1
CDS	1464..7010
Translation	MATPQVKDLVLRSPAGSSDVISFAWPLQIGHGQDKHDNGIDIIDTIKFVCDELPSMSSAFEETNLHQIDTACYKTMTGLVDRFNKAVDSIVALEKGTSLPAERLNKFAHPSLLRHILQLVYNAAVLDPDKLNQYEPFSPEVYGETSYELVQQMLKHVTVSKEDTFIDLGSGVGQVVLQMAGSFPLKTCIGIEKADTPARYAERMDVIFRQYMGWFGKRFCEYKLIKGDFLVDEHRENITSSTLVFVNNFAFGPTVDHQLKERFADLRDGARVVSSKSFCPLNFRITDRNLSDIGTIMHVSEIPPLKGSVSWTCKPVSYYLHVIDRTILERYFQRLKTKGGNDHESVGTVRTTRDRAKREANVGQHHHNNHHSNNHANSNNHQRDREQSNGATATAAHQQRHQSQSPANVSGAGIVLAASGQQAASKTRQQLQHQHNQQQRSLDMESSTESDGDATNGNGGNTTTATNTTSASNGPMTRKVWSDWCSSKGKSSQSDDEENNNSNSNGGSNGGSIGGGSVGRQARATTQKKRKKLTRKAAIASKSAAAAQREAEAAAAAAVSVPSKESSSKEDPPRAASAGPGRKGRMKKGARGRKSLKIVGLEALHKQTVLSTSLDTMTKKLPAAPGTVDQQLTALLTENMSHAELDIPTAPQDTPYALQILLDVFRSQYTSMIEHMKSSAYVPQVQKQIAQEQERMARLKNRASQLDKQIKVLIDDSVALLKVRMNELGIHVNSPNDLIAQAKEIVGRHKDLQHTASRMRNEVTFYEGEQKLLLNKQLKNLPEYQKLCGTVNGKVKLEVPPELSETTAQELVLKEIANTLSQRKKLYAQVSTIEQETSVLQKTAEERSTAATLLAQGTNMIVSTGSSSSSSTTVCASAVTAQSNKLNSVKNSRRNREHRARSQEWPEVPEVGKIQESNPEVLAQKIVETCRQIEAGKFQGAGAPSSQVNGKNKAIIEVPPPPATAPVSIKSSPGHHYKDTTLMPAPKQQQQQQMTLSQLPKCELPGLSTSRKQESPKVANFEDRLKSIITTALNEDQEQRSKAVESSPSPSPLHSPAPKRSKQHPAGAINPAQSLPNNLHNIITVSTQGLMHLNANTTISPITPPLPGPGAGATASTAPPPPANLPYGAYGGAVAKTTISGKYQAAKEPKYSPVRQAPLPPPPSHMASLYPAGQQTTPADLGYQRRRSSVSATSYEHYMVQQQQQLQQQQLMLAAAAHAAQRQQMRVEEQQQQQQHQHHHHHHHHHPQHRLPQHVQHQHPHQHHPNEFKAPPADSHLQRSSSREQLIVEPPQTQPLELLPRASSANSDYSGYRIRPPSRPSSNSSQPDYTQVSPAKMALRRHLSQEKLSQHVTPQATPPLPGHGGAPTSGKTIGDLVNGEIERTLEISHQSIINAAVNMSTSGASFMERAFLNERSNDRLLINLNAQRPERVHVRPLSEESQDPQPTSYAQERGPGLGAGGAAAGGNSNLATLAHVAYAQKAQGGARANAGTAPPATHSSSARSGRDYQPVALPRAELKGSIEAYFHEEQQQKQSKGAGSAGSSSLRGPRLNGANPPLEGLAASLQDHVRARKYKEETEERQRRAAAAASSSAGPPAGMELPTHYAHQAPPAHSYHHHGASINGTPHKVELGIKRSSPLAPHQQPPRPSKLAHYEPPTTQQQHAHAHLYANGQVLPPPPAHDATTPSPTPSSSSSSCGRRSNSNNGKLLVDPPLLMSPEINSLLGDERPLQLSHHQQQQQQMLHHHQSQQQQHLQLTQQQLRVAHLGHGLSHGHSTMPTLGGQRNGNGNAADDVNDLATQRTITNYDPRRRLRTTLSGPTKLSAAHSNQNLNGYVMADSSSSCPTIPQ

Target ORF information:

RefSeq Version	NM_141398.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster grappa (gpp), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141398.4

ORF Insert Sequence:

ATGGCCACAC CCCAAGTCAA GGACTTGGTG TTGCGCTCGC CCGCTGGCTC CTCCGATGTC 
ATTTCCTTCG CCTGGCCCCT GCAGATCGGA CACGGACAGG ATAAGCACGA CAATGGCATC 
GACATCATCG ACACCATTAA GTTCGTCTGC GATGAACTGC CATCGATGTC ATCGGCCTTC 
GAGGAAACCA ATCTCCACCA AATTGACACT GCCTGCTATA AGACTATGAC CGGTCTGGTC 
GATCGCTTCA ACAAGGCCGT CGACAGTATC GTTGCATTGG AAAAAGGAAC TTCACTGCCC 
GCCGAGCGCC TGAACAAGTT CGCTCACCCT AGCCTTCTAA GACACATATT GCAATTAGTT 
TACAATGCCG CCGTACTCGA TCCGGATAAA CTCAACCAGT ACGAGCCCTT CTCGCCAGAG 
GTTTACGGCG AGACGAGCTA TGAGCTGGTG CAGCAGATGC TTAAGCACGT CACTGTCAGC 
AAGGAGGACA CGTTTATCGA TCTCGGCTCG GGGGTGGGCC AGGTGGTTCT GCAGATGGCC 
GGATCGTTTC CCCTGAAAAC CTGCATCGGC ATCGAGAAGG CCGACACGCC GGCGCGATAT 
GCGGAACGCA TGGATGTTAT CTTCCGCCAG TATATGGGAT GGTTTGGAAA ACGCTTTTGC 
GAATACAAGC TGATAAAGGG TGACTTCCTG GTCGATGAGC ATCGTGAAAA CATCACCTCA 
TCGACGCTGG TTTTTGTAAA TAACTTCGCC TTTGGGCCAA CTGTGGACCA TCAGCTCAAG 
GAACGGTTCG CAGATCTTCG CGACGGAGCA CGGGTCGTGT CCTCCAAGTC GTTTTGCCCG 
CTGAACTTTC GGATCACAGA CAGAAACCTC AGTGACATTG GCACCATTAT GCACGTCAGC 
GAAATTCCGC CCCTCAAGGG GTCCGTTTCC TGGACCTGCA AGCCCGTTTC GTATTATCTG 
CATGTGATCG ATCGCACCAT ATTGGAGCGA TACTTTCAGC GCCTGAAAAC TAAGGGCGGC 
AACGATCACG AGAGCGTGGG AACTGTTCGC ACCACACGTG ATCGCGCCAA GCGAGAGGCA 
AACGTTGGCC AACATCATCA CAACAATCAT CATAGCAACA ACCACGCCAA CAGCAACAAC 
CATCAGAGGG ATCGGGAGCA GTCGAACGGA GCTACCGCAA CTGCAGCTCA TCAGCAACGG 
CATCAGTCCC AGTCGCCAGC TAATGTAAGC GGTGCGGGAA TAGTACTTGC AGCTAGCGGG 
CAACAAGCAG CTTCCAAAAC ACGCCAGCAA CTGCAGCATC AGCATAACCA GCAGCAACGC 
AGCTTGGACA TGGAAAGCAG CACGGAAAGC GATGGCGACG CAACGAATGG CAATGGTGGC 
AATACCACCA CTGCCACCAA TACCACCTCC GCCTCCAATG GCCCGATGAC TAGGAAAGTT 
TGGTCCGACT GGTGCAGTTC CAAAGGCAAG AGTTCGCAGT CTGACGACGA GGAGAACAAC 
AACTCGAACA GCAATGGCGG CAGTAATGGT GGTAGCATTG GAGGAGGATC AGTTGGCCGA 
CAGGCTCGGG CTACAACCCA GAAGAAGCGC AAAAAGTTGA CTAGGAAGGC TGCGATAGCC 
AGCAAATCTG CTGCCGCTGC CCAAAGGGAA GCGGAGGCGG CAGCGGCGGC GGCGGTATCC 
GTACCAAGCA AGGAGTCCAG TTCCAAAGAG GATCCACCAA GGGCTGCTAG CGCCGGACCT 
GGTCGCAAAG GACGCATGAA GAAGGGTGCG CGCGGCAGGA AGTCTTTAAA GATTGTTGGA 
CTAGAAGCTC TGCACAAGCA AACAGTGTTG AGTACCTCCT TGGATACCAT GACCAAGAAG 
TTGCCTGCAG CTCCTGGAAC CGTGGATCAA CAGCTTACTG CTTTGCTCAC GGAAAACATG 
TCGCATGCAG AGTTGGATAT TCCAACGGCG CCACAAGACA CGCCTTATGC CTTGCAGATC 
CTTCTAGACG TGTTCCGTTC GCAGTACACA TCGATGATCG AGCACATGAA GTCAAGTGCC 
TATGTGCCTC AAGTTCAGAA GCAGATTGCC CAAGAGCAGG AGCGGATGGC CAGGCTGAAA 
AACAGAGCCA GCCAGTTGGA TAAGCAGATA AAAGTGCTGA TCGATGACAG TGTGGCTTTA 
CTTAAAGTGC GAATGAACGA GCTGGGCATC CATGTAAACT CACCAAACGA TTTGATCGCC 
CAGGCAAAGG AGATTGTGGG TAGACATAAG GATCTACAGC ACACTGCAAG TCGAATGCGA 
AACGAAGTAA CCTTCTATGA GGGCGAACAG AAGCTGCTCC TTAACAAACA ACTAAAAAAT 
CTACCCGAGT ATCAAAAACT CTGTGGTACT GTTAATGGAA AGGTCAAGCT GGAGGTGCCC 
CCGGAACTTT CCGAGACCAC GGCCCAGGAG CTGGTGCTAA AGGAGATCGC CAACACGCTA 
AGTCAACGAA AGAAACTTTA CGCCCAGGTG TCCACCATTG AACAGGAGAC TTCGGTTTTG 
CAGAAGACAG CAGAGGAAAG GTCCACGGCA GCGACGCTCT TGGCACAAGG AACAAATATG 
ATTGTGTCGA CCGGATCATC TTCCTCCTCC TCTACCACTG TTTGTGCAAG TGCAGTTACG 
GCGCAGAGCA ACAAGTTGAA CTCCGTGAAG AACTCTCGTC GCAATCGCGA GCACCGAGCG 
CGTTCCCAGG AGTGGCCAGA GGTTCCCGAA GTGGGTAAAA TCCAGGAAAG CAATCCCGAA 
GTGCTGGCAC AGAAAATAGT CGAGACCTGC CGCCAAATCG AAGCTGGCAA GTTCCAAGGA 
GCAGGTGCGC CCTCATCGCA AGTGAATGGC AAGAATAAAG CAATTATAGA GGTCCCACCA 
CCTCCGGCCA CTGCTCCAGT GAGCATTAAA TCCTCGCCTG GGCACCACTA TAAAGACACA 
ACACTGATGC CAGCACCCAA ACAACAACAG CAACAACAGA TGACGCTTTC CCAGCTTCCG 
AAGTGTGAGC TGCCCGGACT ATCGACTAGT CGCAAACAGG AATCTCCCAA GGTTGCCAAC 
TTTGAGGATC GGTTAAAGAG TATCATCACC ACGGCCTTAA ACGAAGATCA GGAGCAGCGC 
AGCAAGGCAG TGGAGTCGTC ACCCTCTCCC TCACCTCTTC ACAGTCCAGC ACCCAAGCGA 
TCAAAGCAGC ATCCGGCTGG AGCGATAAAC CCTGCCCAGT CGCTGCCCAA CAATCTGCAC 
AACATCATCA CCGTCTCCAC GCAGGGTTTG ATGCACCTTA ATGCCAATAC AACAATATCC 
CCAATAACAC CACCACTGCC GGGTCCAGGT GCAGGAGCAA CCGCATCCAC TGCGCCGCCT 
CCCCCAGCGA ATCTTCCTTA TGGGGCTTAC GGCGGGGCAG TGGCAAAGAC AACGATATCG 
GGGAAGTACC AGGCAGCTAA GGAACCGAAG TATTCGCCAG TTAGACAGGC ACCATTACCT 
CCTCCCCCCT CCCACATGGC ATCTTTGTAT CCAGCTGGAC AGCAGACTAC TCCGGCGGAC 
CTAGGTTACC AGCGGCGACG CAGCTCTGTT AGTGCCACTA GCTACGAACA CTATATGGTA 
CAGCAGCAGC AGCAACTCCA GCAACAACAA CTCATGCTAG CAGCCGCTGC TCATGCGGCC 
CAGCGTCAAC AGATGCGCGT AGAAGAACAA CAGCAGCAGC AGCAGCACCA ACACCACCAC 
CACCACCACC ACCACCATCC CCAACACCGT CTGCCGCAGC ATGTGCAGCA TCAGCATCCG 
CATCAGCATC ATCCCAATGA GTTCAAGGCA CCGCCGGCTG ATAGTCATCT CCAGCGATCG 
AGCAGTCGTG AGCAATTAAT AGTGGAGCCC CCGCAGACGC AACCGCTGGA GCTGCTGCCT 
CGAGCAAGTT CCGCCAATTC GGATTACAGC GGATATCGCA TCCGTCCGCC AAGCAGGCCA 
AGCTCCAACT CCTCGCAACC AGATTACACG CAAGTTTCAC CCGCAAAGAT GGCTCTGCGG 
CGGCATCTGT CGCAGGAGAA GCTGAGCCAA CATGTCACGC CCCAGGCCAC ACCGCCGCTT 
CCTGGTCACG GAGGAGCACC CACATCAGGG AAGACCATTG GCGATTTGGT TAACGGGGAA 
ATCGAGCGAA CGCTTGAGAT TTCGCATCAG AGCATCATTA ATGCGGCTGT GAATATGAGC 
ACTAGTGGCG CTAGCTTCAT GGAGCGGGCC TTCCTTAATG AGCGCTCCAA CGATCGTTTG 
CTGATCAACT TGAATGCCCA ACGACCAGAG AGAGTTCATG TTCGTCCGCT CTCCGAAGAA 
TCGCAGGATC CACAGCCCAC CAGTTATGCT CAGGAGAGAG GACCTGGTCT AGGTGCTGGT 
GGAGCAGCAG CTGGAGGTAA CAGCAATCTG GCCACTTTGG CACATGTAGC CTACGCCCAA 
AAGGCACAGG GCGGCGCTCG AGCCAACGCA GGAACGGCAC CACCCGCAAC CCACTCCTCC 
TCTGCTCGTT CTGGACGTGA CTATCAGCCT GTGGCTTTGC CCCGTGCTGA GCTTAAGGGC 
AGCATTGAGG CCTATTTCCA CGAGGAGCAG CAGCAGAAGC AGTCGAAGGG CGCAGGATCA 
GCAGGATCGT CCTCTTTGCG GGGACCGCGT CTTAATGGTG CCAATCCTCC ATTGGAAGGT 
CTAGCAGCAT CGCTGCAGGA TCATGTCCGT GCCAGAAAGT ACAAGGAGGA GACCGAGGAG 
CGTCAACGAC GTGCAGCAGC CGCTGCCTCG TCTTCGGCGG GACCGCCTGC TGGCATGGAA 
CTGCCAACGC ACTATGCTCA TCAGGCGCCT CCAGCCCACA GCTACCACCA CCATGGTGCA 
AGCATCAATG GAACACCGCA CAAAGTAGAA CTGGGAATAA AACGCAGCTC ACCGCTGGCG 
CCGCATCAGC AACCGCCACG TCCCTCGAAA TTGGCACACT ACGAGCCGCC AACGACGCAG 
CAGCAGCATG CCCATGCCCA CCTGTACGCC AACGGACAAG TGCTACCTCC GCCTCCAGCT 
CATGATGCGA CCACGCCCTC CCCGACGCCC TCGTCATCTT CATCGTCCTG CGGACGTCGA 
AGCAACAGCA ACAATGGAAA GTTGCTCGTG GATCCACCTT TGCTAATGAG TCCCGAGATC 
AATTCACTGT TGGGCGACGA AAGGCCTTTG CAGCTGTCGC ACCACCAACA GCAGCAGCAG 
CAAATGCTGC ATCACCATCA ATCGCAGCAA CAGCAGCATC TGCAGCTGAC CCAACAGCAA 
CTGCGTGTAG CTCATCTTGG ACATGGTCTT AGTCACGGTC ACAGCACCAT GCCCACTTTG 
GGCGGACAAC GCAATGGCAA TGGCAATGCC GCCGACGATG TTAACGATTT GGCAACGCAA 
CGTACTATAA CTAATTACGA TCCCAGACGT CGCCTTCGCA CGACTTTGTC TGGCCCAACG 
AAACTATCAG CTGCCCATAG TAATCAAAAT CTGAACGGAT ATGTTATGGC CGATAGTAGT 
AGTAGTTGCC CCACAATACC CCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gpp ( NM_169142.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731083.1 Drosophila melanogaster grappa (gpp), transcript variant C, mRNA.

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CloneID	OFa04082
Clone ID Related Accession (Same CDS sequence)	NM_169143.2 , NM_141398.4 , NM_169142.2
Accession Version	NM_169142.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	grappa, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 31, 2011 this sequence version replaced NM_169142.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACAC CCCAAGTCAA GGACTTGGTG TTGCGCTCGC CCGCTGGCTC CTCCGATGTC 
ATTTCCTTCG CCTGGCCCCT GCAGATCGGA CACGGACAGG ATAAGCACGA CAATGGCATC 
GACATCATCG ACACCATTAA GTTCGTCTGC GATGAACTGC CATCGATGTC ATCGGCCTTC 
GAGGAAACCA ATCTCCACCA AATTGACACT GCCTGCTATA AGACTATGAC CGGTCTGGTC 
GATCGCTTCA ACAAGGCCGT CGACAGTATC GTTGCATTGG AAAAAGGAAC TTCACTGCCC 
GCCGAGCGCC TGAACAAGTT CGCTCACCCT AGCCTTCTAA GACACATATT GCAATTAGTT 
TACAATGCCG CCGTACTCGA TCCGGATAAA CTCAACCAGT ACGAGCCCTT CTCGCCAGAG 
GTTTACGGCG AGACGAGCTA TGAGCTGGTG CAGCAGATGC TTAAGCACGT CACTGTCAGC 
AAGGAGGACA CGTTTATCGA TCTCGGCTCG GGGGTGGGCC AGGTGGTTCT GCAGATGGCC 
GGATCGTTTC CCCTGAAAAC CTGCATCGGC ATCGAGAAGG CCGACACGCC GGCGCGATAT 
GCGGAACGCA TGGATGTTAT CTTCCGCCAG TATATGGGAT GGTTTGGAAA ACGCTTTTGC 
GAATACAAGC TGATAAAGGG TGACTTCCTG GTCGATGAGC ATCGTGAAAA CATCACCTCA 
TCGACGCTGG TTTTTGTAAA TAACTTCGCC TTTGGGCCAA CTGTGGACCA TCAGCTCAAG 
GAACGGTTCG CAGATCTTCG CGACGGAGCA CGGGTCGTGT CCTCCAAGTC GTTTTGCCCG 
CTGAACTTTC GGATCACAGA CAGAAACCTC AGTGACATTG GCACCATTAT GCACGTCAGC 
GAAATTCCGC CCCTCAAGGG GTCCGTTTCC TGGACCTGCA AGCCCGTTTC GTATTATCTG 
CATGTGATCG ATCGCACCAT ATTGGAGCGA TACTTTCAGC GCCTGAAAAC TAAGGGCGGC 
AACGATCACG AGAGCGTGGG AACTGTTCGC ACCACACGTG ATCGCGCCAA GCGAGAGGCA 
AACGTTGGCC AACATCATCA CAACAATCAT CATAGCAACA ACCACGCCAA CAGCAACAAC 
CATCAGAGGG ATCGGGAGCA GTCGAACGGA GCTACCGCAA CTGCAGCTCA TCAGCAACGG 
CATCAGTCCC AGTCGCCAGC TAATGTAAGC GGTGCGGGAA TAGTACTTGC AGCTAGCGGG 
CAACAAGCAG CTTCCAAAAC ACGCCAGCAA CTGCAGCATC AGCATAACCA GCAGCAACGC 
AGCTTGGACA TGGAAAGCAG CACGGAAAGC GATGGCGACG CAACGAATGG CAATGGTGGC 
AATACCACCA CTGCCACCAA TACCACCTCC GCCTCCAATG GCCCGATGAC TAGGAAAGTT 
TGGTCCGACT GGTGCAGTTC CAAAGGCAAG AGTTCGCAGT CTGACGACGA GGAGAACAAC 
AACTCGAACA GCAATGGCGG CAGTAATGGT GGTAGCATTG GAGGAGGATC AGTTGGCCGA 
CAGGCTCGGG CTACAACCCA GAAGAAGCGC AAAAAGTTGA CTAGGAAGGC TGCGATAGCC 
AGCAAATCTG CTGCCGCTGC CCAAAGGGAA GCGGAGGCGG CAGCGGCGGC GGCGGTATCC 
GTACCAAGCA AGGAGTCCAG TTCCAAAGAG GATCCACCAA GGGCTGCTAG CGCCGGACCT 
GGTCGCAAAG GACGCATGAA GAAGGGTGCG CGCGGCAGGA AGTCTTTAAA GATTGTTGGA 
CTAGAAGCTC TGCACAAGCA AACAGTGTTG AGTACCTCCT TGGATACCAT GACCAAGAAG 
TTGCCTGCAG CTCCTGGAAC CGTGGATCAA CAGCTTACTG CTTTGCTCAC GGAAAACATG 
TCGCATGCAG AGTTGGATAT TCCAACGGCG CCACAAGACA CGCCTTATGC CTTGCAGATC 
CTTCTAGACG TGTTCCGTTC GCAGTACACA TCGATGATCG AGCACATGAA GTCAAGTGCC 
TATGTGCCTC AAGTTCAGAA GCAGATTGCC CAAGAGCAGG AGCGGATGGC CAGGCTGAAA 
AACAGAGCCA GCCAGTTGGA TAAGCAGATA AAAGTGCTGA TCGATGACAG TGTGGCTTTA 
CTTAAAGTGC GAATGAACGA GCTGGGCATC CATGTAAACT CACCAAACGA TTTGATCGCC 
CAGGCAAAGG AGATTGTGGG TAGACATAAG GATCTACAGC ACACTGCAAG TCGAATGCGA 
AACGAAGTAA CCTTCTATGA GGGCGAACAG AAGCTGCTCC TTAACAAACA ACTAAAAAAT 
CTACCCGAGT ATCAAAAACT CTGTGGTACT GTTAATGGAA AGGTCAAGCT GGAGGTGCCC 
CCGGAACTTT CCGAGACCAC GGCCCAGGAG CTGGTGCTAA AGGAGATCGC CAACACGCTA 
AGTCAACGAA AGAAACTTTA CGCCCAGGTG TCCACCATTG AACAGGAGAC TTCGGTTTTG 
CAGAAGACAG CAGAGGAAAG GTCCACGGCA GCGACGCTCT TGGCACAAGG AACAAATATG 
ATTGTGTCGA CCGGATCATC TTCCTCCTCC TCTACCACTG TTTGTGCAAG TGCAGTTACG 
GCGCAGAGCA ACAAGTTGAA CTCCGTGAAG AACTCTCGTC GCAATCGCGA GCACCGAGCG 
CGTTCCCAGG AGTGGCCAGA GGTTCCCGAA GTGGGTAAAA TCCAGGAAAG CAATCCCGAA 
GTGCTGGCAC AGAAAATAGT CGAGACCTGC CGCCAAATCG AAGCTGGCAA GTTCCAAGGA 
GCAGGTGCGC CCTCATCGCA AGTGAATGGC AAGAATAAAG CAATTATAGA GGTCCCACCA 
CCTCCGGCCA CTGCTCCAGT GAGCATTAAA TCCTCGCCTG GGCACCACTA TAAAGACACA 
ACACTGATGC CAGCACCCAA ACAACAACAG CAACAACAGA TGACGCTTTC CCAGCTTCCG 
AAGTGTGAGC TGCCCGGACT ATCGACTAGT CGCAAACAGG AATCTCCCAA GGTTGCCAAC 
TTTGAGGATC GGTTAAAGAG TATCATCACC ACGGCCTTAA ACGAAGATCA GGAGCAGCGC 
AGCAAGGCAG TGGAGTCGTC ACCCTCTCCC TCACCTCTTC ACAGTCCAGC ACCCAAGCGA 
TCAAAGCAGC ATCCGGCTGG AGCGATAAAC CCTGCCCAGT CGCTGCCCAA CAATCTGCAC 
AACATCATCA CCGTCTCCAC GCAGGGTTTG ATGCACCTTA ATGCCAATAC AACAATATCC 
CCAATAACAC CACCACTGCC GGGTCCAGGT GCAGGAGCAA CCGCATCCAC TGCGCCGCCT 
CCCCCAGCGA ATCTTCCTTA TGGGGCTTAC GGCGGGGCAG TGGCAAAGAC AACGATATCG 
GGGAAGTACC AGGCAGCTAA GGAACCGAAG TATTCGCCAG TTAGACAGGC ACCATTACCT 
CCTCCCCCCT CCCACATGGC ATCTTTGTAT CCAGCTGGAC AGCAGACTAC TCCGGCGGAC 
CTAGGTTACC AGCGGCGACG CAGCTCTGTT AGTGCCACTA GCTACGAACA CTATATGGTA 
CAGCAGCAGC AGCAACTCCA GCAACAACAA CTCATGCTAG CAGCCGCTGC TCATGCGGCC 
CAGCGTCAAC AGATGCGCGT AGAAGAACAA CAGCAGCAGC AGCAGCACCA ACACCACCAC 
CACCACCACC ACCACCATCC CCAACACCGT CTGCCGCAGC ATGTGCAGCA TCAGCATCCG 
CATCAGCATC ATCCCAATGA GTTCAAGGCA CCGCCGGCTG ATAGTCATCT CCAGCGATCG 
AGCAGTCGTG AGCAATTAAT AGTGGAGCCC CCGCAGACGC AACCGCTGGA GCTGCTGCCT 
CGAGCAAGTT CCGCCAATTC GGATTACAGC GGATATCGCA TCCGTCCGCC AAGCAGGCCA 
AGCTCCAACT CCTCGCAACC AGATTACACG CAAGTTTCAC CCGCAAAGAT GGCTCTGCGG 
CGGCATCTGT CGCAGGAGAA GCTGAGCCAA CATGTCACGC CCCAGGCCAC ACCGCCGCTT 
CCTGGTCACG GAGGAGCACC CACATCAGGG AAGACCATTG GCGATTTGGT TAACGGGGAA 
ATCGAGCGAA CGCTTGAGAT TTCGCATCAG AGCATCATTA ATGCGGCTGT GAATATGAGC 
ACTAGTGGCG CTAGCTTCAT GGAGCGGGCC TTCCTTAATG AGCGCTCCAA CGATCGTTTG 
CTGATCAACT TGAATGCCCA ACGACCAGAG AGAGTTCATG TTCGTCCGCT CTCCGAAGAA 
TCGCAGGATC CACAGCCCAC CAGTTATGCT CAGGAGAGAG GACCTGGTCT AGGTGCTGGT 
GGAGCAGCAG CTGGAGGTAA CAGCAATCTG GCCACTTTGG CACATGTAGC CTACGCCCAA 
AAGGCACAGG GCGGCGCTCG AGCCAACGCA GGAACGGCAC CACCCGCAAC CCACTCCTCC 
TCTGCTCGTT CTGGACGTGA CTATCAGCCT GTGGCTTTGC CCCGTGCTGA GCTTAAGGGC 
AGCATTGAGG CCTATTTCCA CGAGGAGCAG CAGCAGAAGC AGTCGAAGGG CGCAGGATCA 
GCAGGATCGT CCTCTTTGCG GGGACCGCGT CTTAATGGTG CCAATCCTCC ATTGGAAGGT 
CTAGCAGCAT CGCTGCAGGA TCATGTCCGT GCCAGAAAGT ACAAGGAGGA GACCGAGGAG 
CGTCAACGAC GTGCAGCAGC CGCTGCCTCG TCTTCGGCGG GACCGCCTGC TGGCATGGAA 
CTGCCAACGC ACTATGCTCA TCAGGCGCCT CCAGCCCACA GCTACCACCA CCATGGTGCA 
AGCATCAATG GAACACCGCA CAAAGTAGAA CTGGGAATAA AACGCAGCTC ACCGCTGGCG 
CCGCATCAGC AACCGCCACG TCCCTCGAAA TTGGCACACT ACGAGCCGCC AACGACGCAG 
CAGCAGCATG CCCATGCCCA CCTGTACGCC AACGGACAAG TGCTACCTCC GCCTCCAGCT 
CATGATGCGA CCACGCCCTC CCCGACGCCC TCGTCATCTT CATCGTCCTG CGGACGTCGA 
AGCAACAGCA ACAATGGAAA GTTGCTCGTG GATCCACCTT TGCTAATGAG TCCCGAGATC 
AATTCACTGT TGGGCGACGA AAGGCCTTTG CAGCTGTCGC ACCACCAACA GCAGCAGCAG 
CAAATGCTGC ATCACCATCA ATCGCAGCAA CAGCAGCATC TGCAGCTGAC CCAACAGCAA 
CTGCGTGTAG CTCATCTTGG ACATGGTCTT AGTCACGGTC ACAGCACCAT GCCCACTTTG 
GGCGGACAAC GCAATGGCAA TGGCAATGCC GCCGACGATG TTAACGATTT GGCAACGCAA 
CGTACTATAA CTAATTACGA TCCCAGACGT CGCCTTCGCA CGACTTTGTC TGGCCCAACG 
AAACTATCAG CTGCCCATAG TAATCAAAAT CTGAACGGAT ATGTTATGGC CGATAGTAGT 
AGTAGTTGCC CCACAATACC CCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731083.1
CDS	1850..7396
Translation	MATPQVKDLVLRSPAGSSDVISFAWPLQIGHGQDKHDNGIDIIDTIKFVCDELPSMSSAFEETNLHQIDTACYKTMTGLVDRFNKAVDSIVALEKGTSLPAERLNKFAHPSLLRHILQLVYNAAVLDPDKLNQYEPFSPEVYGETSYELVQQMLKHVTVSKEDTFIDLGSGVGQVVLQMAGSFPLKTCIGIEKADTPARYAERMDVIFRQYMGWFGKRFCEYKLIKGDFLVDEHRENITSSTLVFVNNFAFGPTVDHQLKERFADLRDGARVVSSKSFCPLNFRITDRNLSDIGTIMHVSEIPPLKGSVSWTCKPVSYYLHVIDRTILERYFQRLKTKGGNDHESVGTVRTTRDRAKREANVGQHHHNNHHSNNHANSNNHQRDREQSNGATATAAHQQRHQSQSPANVSGAGIVLAASGQQAASKTRQQLQHQHNQQQRSLDMESSTESDGDATNGNGGNTTTATNTTSASNGPMTRKVWSDWCSSKGKSSQSDDEENNNSNSNGGSNGGSIGGGSVGRQARATTQKKRKKLTRKAAIASKSAAAAQREAEAAAAAAVSVPSKESSSKEDPPRAASAGPGRKGRMKKGARGRKSLKIVGLEALHKQTVLSTSLDTMTKKLPAAPGTVDQQLTALLTENMSHAELDIPTAPQDTPYALQILLDVFRSQYTSMIEHMKSSAYVPQVQKQIAQEQERMARLKNRASQLDKQIKVLIDDSVALLKVRMNELGIHVNSPNDLIAQAKEIVGRHKDLQHTASRMRNEVTFYEGEQKLLLNKQLKNLPEYQKLCGTVNGKVKLEVPPELSETTAQELVLKEIANTLSQRKKLYAQVSTIEQETSVLQKTAEERSTAATLLAQGTNMIVSTGSSSSSSTTVCASAVTAQSNKLNSVKNSRRNREHRARSQEWPEVPEVGKIQESNPEVLAQKIVETCRQIEAGKFQGAGAPSSQVNGKNKAIIEVPPPPATAPVSIKSSPGHHYKDTTLMPAPKQQQQQQMTLSQLPKCELPGLSTSRKQESPKVANFEDRLKSIITTALNEDQEQRSKAVESSPSPSPLHSPAPKRSKQHPAGAINPAQSLPNNLHNIITVSTQGLMHLNANTTISPITPPLPGPGAGATASTAPPPPANLPYGAYGGAVAKTTISGKYQAAKEPKYSPVRQAPLPPPPSHMASLYPAGQQTTPADLGYQRRRSSVSATSYEHYMVQQQQQLQQQQLMLAAAAHAAQRQQMRVEEQQQQQQHQHHHHHHHHHPQHRLPQHVQHQHPHQHHPNEFKAPPADSHLQRSSSREQLIVEPPQTQPLELLPRASSANSDYSGYRIRPPSRPSSNSSQPDYTQVSPAKMALRRHLSQEKLSQHVTPQATPPLPGHGGAPTSGKTIGDLVNGEIERTLEISHQSIINAAVNMSTSGASFMERAFLNERSNDRLLINLNAQRPERVHVRPLSEESQDPQPTSYAQERGPGLGAGGAAAGGNSNLATLAHVAYAQKAQGGARANAGTAPPATHSSSARSGRDYQPVALPRAELKGSIEAYFHEEQQQKQSKGAGSAGSSSLRGPRLNGANPPLEGLAASLQDHVRARKYKEETEERQRRAAAAASSSAGPPAGMELPTHYAHQAPPAHSYHHHGASINGTPHKVELGIKRSSPLAPHQQPPRPSKLAHYEPPTTQQQHAHAHLYANGQVLPPPPAHDATTPSPTPSSSSSSCGRRSNSNNGKLLVDPPLLMSPEINSLLGDERPLQLSHHQQQQQQMLHHHQSQQQQHLQLTQQQLRVAHLGHGLSHGHSTMPTLGGQRNGNGNAADDVNDLATQRTITNYDPRRRLRTTLSGPTKLSAAHSNQNLNGYVMADSSSSCPTIPQ

Target ORF information:

RefSeq Version	NM_169142.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster grappa (gpp), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169142.2

ORF Insert Sequence:

ATGGCCACAC CCCAAGTCAA GGACTTGGTG TTGCGCTCGC CCGCTGGCTC CTCCGATGTC 
ATTTCCTTCG CCTGGCCCCT GCAGATCGGA CACGGACAGG ATAAGCACGA CAATGGCATC 
GACATCATCG ACACCATTAA GTTCGTCTGC GATGAACTGC CATCGATGTC ATCGGCCTTC 
GAGGAAACCA ATCTCCACCA AATTGACACT GCCTGCTATA AGACTATGAC CGGTCTGGTC 
GATCGCTTCA ACAAGGCCGT CGACAGTATC GTTGCATTGG AAAAAGGAAC TTCACTGCCC 
GCCGAGCGCC TGAACAAGTT CGCTCACCCT AGCCTTCTAA GACACATATT GCAATTAGTT 
TACAATGCCG CCGTACTCGA TCCGGATAAA CTCAACCAGT ACGAGCCCTT CTCGCCAGAG 
GTTTACGGCG AGACGAGCTA TGAGCTGGTG CAGCAGATGC TTAAGCACGT CACTGTCAGC 
AAGGAGGACA CGTTTATCGA TCTCGGCTCG GGGGTGGGCC AGGTGGTTCT GCAGATGGCC 
GGATCGTTTC CCCTGAAAAC CTGCATCGGC ATCGAGAAGG CCGACACGCC GGCGCGATAT 
GCGGAACGCA TGGATGTTAT CTTCCGCCAG TATATGGGAT GGTTTGGAAA ACGCTTTTGC 
GAATACAAGC TGATAAAGGG TGACTTCCTG GTCGATGAGC ATCGTGAAAA CATCACCTCA 
TCGACGCTGG TTTTTGTAAA TAACTTCGCC TTTGGGCCAA CTGTGGACCA TCAGCTCAAG 
GAACGGTTCG CAGATCTTCG CGACGGAGCA CGGGTCGTGT CCTCCAAGTC GTTTTGCCCG 
CTGAACTTTC GGATCACAGA CAGAAACCTC AGTGACATTG GCACCATTAT GCACGTCAGC 
GAAATTCCGC CCCTCAAGGG GTCCGTTTCC TGGACCTGCA AGCCCGTTTC GTATTATCTG 
CATGTGATCG ATCGCACCAT ATTGGAGCGA TACTTTCAGC GCCTGAAAAC TAAGGGCGGC 
AACGATCACG AGAGCGTGGG AACTGTTCGC ACCACACGTG ATCGCGCCAA GCGAGAGGCA 
AACGTTGGCC AACATCATCA CAACAATCAT CATAGCAACA ACCACGCCAA CAGCAACAAC 
CATCAGAGGG ATCGGGAGCA GTCGAACGGA GCTACCGCAA CTGCAGCTCA TCAGCAACGG 
CATCAGTCCC AGTCGCCAGC TAATGTAAGC GGTGCGGGAA TAGTACTTGC AGCTAGCGGG 
CAACAAGCAG CTTCCAAAAC ACGCCAGCAA CTGCAGCATC AGCATAACCA GCAGCAACGC 
AGCTTGGACA TGGAAAGCAG CACGGAAAGC GATGGCGACG CAACGAATGG CAATGGTGGC 
AATACCACCA CTGCCACCAA TACCACCTCC GCCTCCAATG GCCCGATGAC TAGGAAAGTT 
TGGTCCGACT GGTGCAGTTC CAAAGGCAAG AGTTCGCAGT CTGACGACGA GGAGAACAAC 
AACTCGAACA GCAATGGCGG CAGTAATGGT GGTAGCATTG GAGGAGGATC AGTTGGCCGA 
CAGGCTCGGG CTACAACCCA GAAGAAGCGC AAAAAGTTGA CTAGGAAGGC TGCGATAGCC 
AGCAAATCTG CTGCCGCTGC CCAAAGGGAA GCGGAGGCGG CAGCGGCGGC GGCGGTATCC 
GTACCAAGCA AGGAGTCCAG TTCCAAAGAG GATCCACCAA GGGCTGCTAG CGCCGGACCT 
GGTCGCAAAG GACGCATGAA GAAGGGTGCG CGCGGCAGGA AGTCTTTAAA GATTGTTGGA 
CTAGAAGCTC TGCACAAGCA AACAGTGTTG AGTACCTCCT TGGATACCAT GACCAAGAAG 
TTGCCTGCAG CTCCTGGAAC CGTGGATCAA CAGCTTACTG CTTTGCTCAC GGAAAACATG 
TCGCATGCAG AGTTGGATAT TCCAACGGCG CCACAAGACA CGCCTTATGC CTTGCAGATC 
CTTCTAGACG TGTTCCGTTC GCAGTACACA TCGATGATCG AGCACATGAA GTCAAGTGCC 
TATGTGCCTC AAGTTCAGAA GCAGATTGCC CAAGAGCAGG AGCGGATGGC CAGGCTGAAA 
AACAGAGCCA GCCAGTTGGA TAAGCAGATA AAAGTGCTGA TCGATGACAG TGTGGCTTTA 
CTTAAAGTGC GAATGAACGA GCTGGGCATC CATGTAAACT CACCAAACGA TTTGATCGCC 
CAGGCAAAGG AGATTGTGGG TAGACATAAG GATCTACAGC ACACTGCAAG TCGAATGCGA 
AACGAAGTAA CCTTCTATGA GGGCGAACAG AAGCTGCTCC TTAACAAACA ACTAAAAAAT 
CTACCCGAGT ATCAAAAACT CTGTGGTACT GTTAATGGAA AGGTCAAGCT GGAGGTGCCC 
CCGGAACTTT CCGAGACCAC GGCCCAGGAG CTGGTGCTAA AGGAGATCGC CAACACGCTA 
AGTCAACGAA AGAAACTTTA CGCCCAGGTG TCCACCATTG AACAGGAGAC TTCGGTTTTG 
CAGAAGACAG CAGAGGAAAG GTCCACGGCA GCGACGCTCT TGGCACAAGG AACAAATATG 
ATTGTGTCGA CCGGATCATC TTCCTCCTCC TCTACCACTG TTTGTGCAAG TGCAGTTACG 
GCGCAGAGCA ACAAGTTGAA CTCCGTGAAG AACTCTCGTC GCAATCGCGA GCACCGAGCG 
CGTTCCCAGG AGTGGCCAGA GGTTCCCGAA GTGGGTAAAA TCCAGGAAAG CAATCCCGAA 
GTGCTGGCAC AGAAAATAGT CGAGACCTGC CGCCAAATCG AAGCTGGCAA GTTCCAAGGA 
GCAGGTGCGC CCTCATCGCA AGTGAATGGC AAGAATAAAG CAATTATAGA GGTCCCACCA 
CCTCCGGCCA CTGCTCCAGT GAGCATTAAA TCCTCGCCTG GGCACCACTA TAAAGACACA 
ACACTGATGC CAGCACCCAA ACAACAACAG CAACAACAGA TGACGCTTTC CCAGCTTCCG 
AAGTGTGAGC TGCCCGGACT ATCGACTAGT CGCAAACAGG AATCTCCCAA GGTTGCCAAC 
TTTGAGGATC GGTTAAAGAG TATCATCACC ACGGCCTTAA ACGAAGATCA GGAGCAGCGC 
AGCAAGGCAG TGGAGTCGTC ACCCTCTCCC TCACCTCTTC ACAGTCCAGC ACCCAAGCGA 
TCAAAGCAGC ATCCGGCTGG AGCGATAAAC CCTGCCCAGT CGCTGCCCAA CAATCTGCAC 
AACATCATCA CCGTCTCCAC GCAGGGTTTG ATGCACCTTA ATGCCAATAC AACAATATCC 
CCAATAACAC CACCACTGCC GGGTCCAGGT GCAGGAGCAA CCGCATCCAC TGCGCCGCCT 
CCCCCAGCGA ATCTTCCTTA TGGGGCTTAC GGCGGGGCAG TGGCAAAGAC AACGATATCG 
GGGAAGTACC AGGCAGCTAA GGAACCGAAG TATTCGCCAG TTAGACAGGC ACCATTACCT 
CCTCCCCCCT CCCACATGGC ATCTTTGTAT CCAGCTGGAC AGCAGACTAC TCCGGCGGAC 
CTAGGTTACC AGCGGCGACG CAGCTCTGTT AGTGCCACTA GCTACGAACA CTATATGGTA 
CAGCAGCAGC AGCAACTCCA GCAACAACAA CTCATGCTAG CAGCCGCTGC TCATGCGGCC 
CAGCGTCAAC AGATGCGCGT AGAAGAACAA CAGCAGCAGC AGCAGCACCA ACACCACCAC 
CACCACCACC ACCACCATCC CCAACACCGT CTGCCGCAGC ATGTGCAGCA TCAGCATCCG 
CATCAGCATC ATCCCAATGA GTTCAAGGCA CCGCCGGCTG ATAGTCATCT CCAGCGATCG 
AGCAGTCGTG AGCAATTAAT AGTGGAGCCC CCGCAGACGC AACCGCTGGA GCTGCTGCCT 
CGAGCAAGTT CCGCCAATTC GGATTACAGC GGATATCGCA TCCGTCCGCC AAGCAGGCCA 
AGCTCCAACT CCTCGCAACC AGATTACACG CAAGTTTCAC CCGCAAAGAT GGCTCTGCGG 
CGGCATCTGT CGCAGGAGAA GCTGAGCCAA CATGTCACGC CCCAGGCCAC ACCGCCGCTT 
CCTGGTCACG GAGGAGCACC CACATCAGGG AAGACCATTG GCGATTTGGT TAACGGGGAA 
ATCGAGCGAA CGCTTGAGAT TTCGCATCAG AGCATCATTA ATGCGGCTGT GAATATGAGC 
ACTAGTGGCG CTAGCTTCAT GGAGCGGGCC TTCCTTAATG AGCGCTCCAA CGATCGTTTG 
CTGATCAACT TGAATGCCCA ACGACCAGAG AGAGTTCATG TTCGTCCGCT CTCCGAAGAA 
TCGCAGGATC CACAGCCCAC CAGTTATGCT CAGGAGAGAG GACCTGGTCT AGGTGCTGGT 
GGAGCAGCAG CTGGAGGTAA CAGCAATCTG GCCACTTTGG CACATGTAGC CTACGCCCAA 
AAGGCACAGG GCGGCGCTCG AGCCAACGCA GGAACGGCAC CACCCGCAAC CCACTCCTCC 
TCTGCTCGTT CTGGACGTGA CTATCAGCCT GTGGCTTTGC CCCGTGCTGA GCTTAAGGGC 
AGCATTGAGG CCTATTTCCA CGAGGAGCAG CAGCAGAAGC AGTCGAAGGG CGCAGGATCA 
GCAGGATCGT CCTCTTTGCG GGGACCGCGT CTTAATGGTG CCAATCCTCC ATTGGAAGGT 
CTAGCAGCAT CGCTGCAGGA TCATGTCCGT GCCAGAAAGT ACAAGGAGGA GACCGAGGAG 
CGTCAACGAC GTGCAGCAGC CGCTGCCTCG TCTTCGGCGG GACCGCCTGC TGGCATGGAA 
CTGCCAACGC ACTATGCTCA TCAGGCGCCT CCAGCCCACA GCTACCACCA CCATGGTGCA 
AGCATCAATG GAACACCGCA CAAAGTAGAA CTGGGAATAA AACGCAGCTC ACCGCTGGCG 
CCGCATCAGC AACCGCCACG TCCCTCGAAA TTGGCACACT ACGAGCCGCC AACGACGCAG 
CAGCAGCATG CCCATGCCCA CCTGTACGCC AACGGACAAG TGCTACCTCC GCCTCCAGCT 
CATGATGCGA CCACGCCCTC CCCGACGCCC TCGTCATCTT CATCGTCCTG CGGACGTCGA 
AGCAACAGCA ACAATGGAAA GTTGCTCGTG GATCCACCTT TGCTAATGAG TCCCGAGATC 
AATTCACTGT TGGGCGACGA AAGGCCTTTG CAGCTGTCGC ACCACCAACA GCAGCAGCAG 
CAAATGCTGC ATCACCATCA ATCGCAGCAA CAGCAGCATC TGCAGCTGAC CCAACAGCAA 
CTGCGTGTAG CTCATCTTGG ACATGGTCTT AGTCACGGTC ACAGCACCAT GCCCACTTTG 
GGCGGACAAC GCAATGGCAA TGGCAATGCC GCCGACGATG TTAACGATTT GGCAACGCAA 
CGTACTATAA CTAATTACGA TCCCAGACGT CGCCTTCGCA CGACTTTGTC TGGCCCAACG 
AAACTATCAG CTGCCCATAG TAATCAAAAT CTGAACGGAT ATGTTATGGC CGATAGTAGT 
AGTAGTTGCC CCACAATACC CCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gpp ( NM_169144.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731085.2 Drosophila melanogaster grappa (gpp), transcript variant D, mRNA.

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CloneID	OFa04083
Clone ID Related Accession (Same CDS sequence)	NM_169144.2
Accession Version	NM_169144.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	grappa, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 31, 2011 this sequence version replaced NM_169144.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACAC CCCAAGTCAA GGACTTGGTG TTGCGCTCGC CCGCTGGCTC CTCCGATGTC 
ATTTCCTTCG CCTGGCCCCT GCAGATCGGA CACGGACAGG ATAAGCACGA CAATGGCATC 
GACATCATCG ACACCATTAA GTTCGTCTGC GATGAACTGC CATCGATGTC ATCGGCCTTC 
GAGGAAACCA ATCTCCACCA AATTGACACT GCCTGCTATA AGACTATGAC CGGTCTGGTC 
GATCGCTTCA ACAAGGCCGT CGACAGTATC GTTGCATTGG AAAAAGGAAC TTCACTGCCC 
GCCGAGCGCC TGAACAAGTT CGCTCACCCT AGCCTTCTAA GACACATATT GCAATTAGTT 
TACAATGCCG CCGTACTCGA TCCGGATAAA CTCAACCAGT ACGAGCCCTT CTCGCCAGAG 
GTTTACGGCG AGACGAGCTA TGAGCTGGTG CAGCAGATGC TTAAGCACGT CACTGTCAGC 
AAGGAGGACA CGTTTATCGA TCTCGGCTCG GGGGTGGGCC AGGTGGTTCT GCAGATGGCC 
GGATCGTTTC CCCTGAAAAC CTGCATCGGC ATCGAGAAGG CCGACACGCC GGCGCGATAT 
GCGGAACGCA TGGATGTTAT CTTCCGCCAG TATATGGGAT GGTTTGGAAA ACGCTTTTGC 
GAATACAAGC TGATAAAGGG TGACTTCCTG GTCGATGAGC ATCGTGAAAA CATCACCTCA 
TCGACGCTGG TTTTTGTAAA TAACTTCGCC TTTGGGCCAA CTGTGGACCA TCAGCTCAAG 
GAACGGTTCG CAGATCTTCG CGACGGAGCA CGGGTCGTGT CCTCCAAGTC GTTTTGCCCG 
CTGAACTTTC GGATCACAGA CAGAAACCTC AGTGACATTG GCACCATTAT GCACGTCAGC 
GAAATTCCGC CCCTCAAGGG GTCCGTTTCC TGGACCTGCA AGCCCGTTTC GTATTATCTG 
CATGTGATCG ATCGCACCAT ATTGGAGCGA TACTTTCAGC GCCTGAAAAC TAAGGGCGGC 
AACGATCACG AGAGCGTGGG AACTGTTCGC ACCACACGTG ATCGCGCCAA GCGAGAGGCA 
AACGTTGGCC AACATCATCA CAACAATCAT CATAGCAACA ACCACGCCAA CAGCAACAAC 
CATCAGAGGG ATCGGGAGCA GTCGAACGGA GCTACCGCAA CTGCAGCTCA TCAGCAACGG 
CATCAGTCCC AGTCGCCAGC TAATGTAAGC GGTGCGGGAA TAGTACTTGC AGCTAGCGGG 
CAACAAGCAG CTTCCAAAAC ACGCCAGCAA CTGCAGCATC AGCATAACCA GCAGCAACGC 
AGCTTGGACA TGGAAAGCAG CACGGAAAGC GATGGCGACG CAACGAATGG CAATGGTGGC 
AATACCACCA CTGCCACCAA TACCACCTCC GCCTCCAATG GCCCGATGAC TAGGAAAGTT 
TGGTCCGACT GGTGCAGTTC CAAAGGCAAG AGTTCGCAGT CTGACGACGA GGAGAACAAC 
AACTCGAACA GCAATGGCGG CAGTAATGGT GGTAGCATTG GAGGAGGATC AGTTGGCCGA 
CAGGCTCGGG CTACAACCCA GAAGAAGCGC AAAAAGTTGA CTAGGAAGGC TGCGATAGCC 
AGCAAATCTG CTGCCGCTGC CCAAAGGGAA GCGGAGGCGG CAGCGGCGGC GGCGGTATCC 
GTACCAAGCA AGGAGTCCAG TTCCAAAGAG GATCCACCAA GGGCTGCTAG CGCCGGACCT 
GGTCGCAAAG GACGCATGAA GAAGGGTGCG CGCGGCAGGA AGTCTTTAAA GATTGTTGGA 
CTAGAAGCTC TGCACAAGCA AACAGTGTTG AGTACCTCCT TGGATACCAT GACCAAGAAG 
TTGCCTGCAG CTCCTGGAAC CGTGGATCAA CAGCTTACTG CTTTGCTCAC GGAAAACATG 
TCGCATGCAG AGTTGGATAT TCCAACGGCG CCACAAGACA CGCCTTATGC CTTGCAGATC 
CTTCTAGACG TGTTCCGTTC GCAGTACACA TCGATGATCG AGCACATGAA GTCAAGTGCC 
TATGTGCCTC AAGTTCAGAA GCAGATTGCC CAAGAGCAGG AGCGGATGGC CAGGCTGAAA 
AACAGAGCCA GCCAGTTGGA TAAGCAGATA AAAGTGCTGA TCGATGACAG TGTGGCTTTA 
CTTAAAGTGC GAATGAACGA GCTGGGCATC CATGTAAACT CACCAAACGA TTTGATCGCC 
CAGGCAAAGG AGATTGTGGG TAGACATAAG GATCTACAGC ACACTGCAAG TCGAATGCGA 
AACGAAGTAA CCTTCTATGA GGGCGAACAG AAGCTGCTCC TTAACAAACA ACTAAAAAAT 
CTACCCGAGT ATCAAAAACT CTGTGGTACT GTTAATGGAA AGGTCAAGCT GGAGGTGCCC 
CCGGAACTTT CCGAGACCAC GGCCCAGGAG CTGGTGCTAA AGGAGATCGC CAACACGCTA 
AGTCAACGAA AGAAACTTTA CGCCCAGGTG TCCACCATTG AACAGGAGAC TTCGGTTTTG 
CAGAAGACAG CAGAGGAAAG GTCCACGGCA GCGACGCTCT TGGCACAAGG AACAAATATG 
ATTGTGTCGA CCGGATCATC TTCCTCCTCC TCTACCACTG TTTGTGCAAG TGCAGTTACG 
GCGCAGAGCA ACAAGTTGAA CTCCGTGAAG AACTCTCGTC GCAATCGCGA GCACCGAGCG 
CGTTCCCAGG AGTGGCCAGA GGTTCCCGAA GTGGGTAAAA TCCAGGAAAG CAATCCCGAA 
GTGCTGGCAC AGAAAATAGT CGAGACCTGC CGCCAAATCG AAGCTGGCAA GTTCCAAGGA 
GCAGGTGCGC CCTCATCGCA AGTGAATGGC AAGAATAAAG CAATTATAGA GGTCCCACCA 
CCTCCGGCCA CTGCTCCAGT GAGCATTAAA TCCTCGCCTG GGCACCACTA TAAAGACACA 
ACACTGATGC CAGCACCCAA ACAACAACAG CAACAACAGA TGACGCTTTC CCAGCTTCCG 
AAGTGTGAGC TGCCCGGACT ATCGACTAGT CGCAAACAGG AATCTCCCAA GGTTGCCAAC 
TTTGAGGATC GGTTAAAGAG TATCATCACC ACGGCCTTAA ACGAAGATCA GGAGCAGCGC 
AGCAAGGCAG TGGAGTCGTC ACCCTCTCCC TCACCTCTTC ACAGTCCAGC ACCCAAGCGA 
TCAAAGCAGC ATCCGGCTGG AGCGATAAAC CCTGCCCAGT CGCTGCCCAA CAATCTGCAC 
AACATCATCA CCGTCTCCAC GCAGGGTTTG ATGCACCTTA ATGCCAATAC AACAATATCC 
CCAATAACAC CACCACTGCC GGGTCCAGGT GCAGGAGCAA CCGCATCCAC TGCGCCGCCT 
CCCCCAGCGA ATCTTCCTTA TGGGGCTTAC GGCGGGGCAG TGGCAAAGAC AACGATATCG 
GGGAAGTACC AGGCAGCTAA GGAACCGAAG TATTCGCCAG TTAGACAGGC ACCATTACCT 
CCTCCCCCCT CCCACATGGC ATCTTTGTAT CCAGCTGGAC AGCAGACTAC TCCGGCGGAC 
CTAGGTTACC AGCGGCGACG CAGCTCTGTT AGTGCCACTA GCTACGAACA CTATATGGTA 
CAGCAGCAGC AGCAACTCCA GCAACAACAA CTCATGCTAG CAGCCGCTGC TCATGCGGCC 
CAGCGTCAAC AGATGCGCGT AGAAGAACAA CAGCAGCAGC AGCAGCACCA ACACCACCAC 
CACCACCACC ACCACCATCC CCAACACCGT CTGCCGCAGC ATGTGCAGCA TCAGCATCCG 
CATCAGCATC ATCCCAATGA GTTCAAGGCA CCGCCGGCTG ATAGTCATCT CCAGCGATCG 
AGCAGTCGTG AGCAATTAAT AGTGGAGCCC CCGCAGACGC AACCGCTGGA GCTGCTGCCT 
CGAGCAAGTT CCGCCAATTC GGATTACAGC GGATATCGCA TCCGTCCGCC AAGCAGGCCA 
AGCTCCAACT CCTCGCAACC AGATTACACG CAAGTTTCAC CCGCAAAGAT GGCTCTGCGG 
CGGCATCTGT CGCAGGAGAA GCTGAGCCAA CATGTCACGC CCCAGGCCAC ACCGCCGCTT 
CCTGGTCACG GAGGAGCACC CACATCAGGG AAGACCATTG GCGATTTGGT TAACGGGGAA 
ATCGAGCGAA CGCTTGAGAT TTCGCATCAG AGCATCATTA ATGCGGCTGT GAATATGAGC 
ACTAGTGGCG CTAGCTTCAT GGAGCGGGCC TTCCTTAATG AGCGCTCCAA CGATCGTTTG 
CTGATCAACT TGAATGCCCA ACGACCAGAG AGAGTTCATG TTCGTCCGCT CTCCGAAGAA 
TCGCAGGATC CACAGCCCAC CAGTTATGCT CAGGAGAGAG GACCTGGTCT AGGTGCTGGT 
GGAGCAGCAG CTGGAGGTAA CAGCAATCTG GCCACTTTGG CACATGTAGC CTACGCCCAA 
AAGGCACAGG GCGGCGCTCG AGCCAACGCA GGAACGGCAC CACCCGCAAC CCACTCCTCC 
TCTGCTCGTT CTGGACGTGA CTATCAGCCT GTGGCTTTGC CCCGTGCTGA GCTTAAGGGC 
AGCATTGAGG CCTATTTCCA CGAGGAGCAG CAGCAGAAGC AGTCGAAGGG CGCAGGATCA 
GCAGGATCGT CCTCTTTGCG GGGACCGCGT CTTAATGGTG CCAATCCTCC ATTGGAAGGT 
CTAGCAGCAT CGCTGCAGGA TCATGTCCGT GCCAGAAAGT ACAAGGAGGA GACCGAGGAG 
CGTCAACGAC GTGCAGCAGC CGCTGCCTCG TCTTCGGCGG GACCGCCTGC TGGCATGGAA 
CTGCCAACGC ACTATGCTCA TCAGGCGCCT CCAGCCCACA GCTACCACCA CCATGGTGCA 
AGCATCAATG GAACACCGCA CAAAGTAGAA CTGGGAATAA AACGCAGCTC ACCGCTGGCG 
CCGCATCAGC AACCGCCACG TCCCTCGAAA TTGGCACACT ACGAGCCGCC AACGACGCAG 
CAGCAGCATG CCCATGCCCA CCTGTACGCC AACGGACAAG TGCTACCTCC GCCTCCAGCT 
CATGATGCGA CCACGCCCTC CCCGACGCCC TCGTCATCTT CATCGTCCTG CGGACGTCGA 
AGCAACAGCA ACAATGGAAA GTTGCTCGTG GATCCACCTT TGCTAATGAG TCCCGAGATC 
AATTCACTGT TGGGCGACGA AAGGCCTTTG CAGCTGTCGC ACCACCAACA GCAGCAGCAG 
CAAATGCTGC ATCACCATCA ATCGCAGCAA CAGCAGCATC TGCAGCTGAC CCAACAGCAA 
CTGCGTGTAG CTCATCTTGG ACATGGTCTT AGTCACGGTC ACAGCACCAT GCCCACTTTG 
GGCGGACAAC GCAATGGCAA TGGCAATGCC GCCGACGATG ACGATGTTAT TGCCGCTGAT 
GATGAGACCC ACTGGCAGCA TCGAGTCAGC TCTGGTTTCG ACCGCCTCGT GGCCTTCGCC 
AGCACTGAAT TGGATAAGAC CAGGCGAAGC ATCGATGGCG ATACCGTGCC GGCCTCGATT 
AGTTGCAACA CGTCTCCGGA CTCGGGCATT ACGCACAGCA GCAGCAACTC AGATGCAGTG 
CGCACATTTT TGTCCACCTC GTCCAGCTCC TCGCAGCTGG AGCTGCCCAT AGGAAGCGGT 
GGTAGTAGCT GCAACAGCCT CTACAACCAT CATGCCCAGC ACAACAACAT TGGCATTGGC 
GGCAGCGGAG GCAGCCACCC ACATCAGCAA CAGCAACAGT TGCAGGCGCA GCACCAACAG 
CAGCAGCACC ATCTTTCCGA TCACATGAGC GACAGTAGCA TGAAATCCTC GGCATCCTCC 
TCGCTGCATG CTGGTAGCTC CCTTAAGACC GTGTCGCCAG TGGATCCCAG TGCCATCGAG 
TCGCCGCCAC TCTCGGACGT TGGATTGCCC AGAACTCCCA GTCCCAGTGC AGCCAGTGTG 
GCCACTCCAC CACTGGCCAG TGGACCACCC GTGTCCGGGG ACTTAGGTGG TATACCTCTG 
AAGTACCAGC GCAAGTCCAA GACCAGTTCG GAAAAGCACT ACAAAAAGAA GTTCTGCGAA 
AGAAACTGGG AGTACGACTG TGATGAACTG CTGGCCTACT CCAACTCCAG TGCACCGCCG 
ACGGCAGCAG ATGCAGCGGG CAATGGGCCA CTTAACTTGG ATGCTCCCAA TACTGTGGGC 
GGAGCACCTG CACCGCTCTT GGGAAAATCG GGAAAGTCAC CCAAGGAAAA GTCTTCACCG 
CACCACACGG GCTTAAACCA TCAGCAGCAG CACCATCACA AGTCATCCAA ATTTCGGCCA 
AAGGGCAAGG ACTGGGACTG GTCAATGGAC AGTCGGAGCC AAACGGGCGA GCTGCTGGCC 
GCCAGTAACA ACATGAACAT TAACAACAAC TCAGCCACCA CAACGAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731085.2
CDS	1464..7877
Translation	MATPQVKDLVLRSPAGSSDVISFAWPLQIGHGQDKHDNGIDIIDTIKFVCDELPSMSSAFEETNLHQIDTACYKTMTGLVDRFNKAVDSIVALEKGTSLPAERLNKFAHPSLLRHILQLVYNAAVLDPDKLNQYEPFSPEVYGETSYELVQQMLKHVTVSKEDTFIDLGSGVGQVVLQMAGSFPLKTCIGIEKADTPARYAERMDVIFRQYMGWFGKRFCEYKLIKGDFLVDEHRENITSSTLVFVNNFAFGPTVDHQLKERFADLRDGARVVSSKSFCPLNFRITDRNLSDIGTIMHVSEIPPLKGSVSWTCKPVSYYLHVIDRTILERYFQRLKTKGGNDHESVGTVRTTRDRAKREANVGQHHHNNHHSNNHANSNNHQRDREQSNGATATAAHQQRHQSQSPANVSGAGIVLAASGQQAASKTRQQLQHQHNQQQRSLDMESSTESDGDATNGNGGNTTTATNTTSASNGPMTRKVWSDWCSSKGKSSQSDDEENNNSNSNGGSNGGSIGGGSVGRQARATTQKKRKKLTRKAAIASKSAAAAQREAEAAAAAAVSVPSKESSSKEDPPRAASAGPGRKGRMKKGARGRKSLKIVGLEALHKQTVLSTSLDTMTKKLPAAPGTVDQQLTALLTENMSHAELDIPTAPQDTPYALQILLDVFRSQYTSMIEHMKSSAYVPQVQKQIAQEQERMARLKNRASQLDKQIKVLIDDSVALLKVRMNELGIHVNSPNDLIAQAKEIVGRHKDLQHTASRMRNEVTFYEGEQKLLLNKQLKNLPEYQKLCGTVNGKVKLEVPPELSETTAQELVLKEIANTLSQRKKLYAQVSTIEQETSVLQKTAEERSTAATLLAQGTNMIVSTGSSSSSSTTVCASAVTAQSNKLNSVKNSRRNREHRARSQEWPEVPEVGKIQESNPEVLAQKIVETCRQIEAGKFQGAGAPSSQVNGKNKAIIEVPPPPATAPVSIKSSPGHHYKDTTLMPAPKQQQQQQMTLSQLPKCELPGLSTSRKQESPKVANFEDRLKSIITTALNEDQEQRSKAVESSPSPSPLHSPAPKRSKQHPAGAINPAQSLPNNLHNIITVSTQGLMHLNANTTISPITPPLPGPGAGATASTAPPPPANLPYGAYGGAVAKTTISGKYQAAKEPKYSPVRQAPLPPPPSHMASLYPAGQQTTPADLGYQRRRSSVSATSYEHYMVQQQQQLQQQQLMLAAAAHAAQRQQMRVEEQQQQQQHQHHHHHHHHHPQHRLPQHVQHQHPHQHHPNEFKAPPADSHLQRSSSREQLIVEPPQTQPLELLPRASSANSDYSGYRIRPPSRPSSNSSQPDYTQVSPAKMALRRHLSQEKLSQHVTPQATPPLPGHGGAPTSGKTIGDLVNGEIERTLEISHQSIINAAVNMSTSGASFMERAFLNERSNDRLLINLNAQRPERVHVRPLSEESQDPQPTSYAQERGPGLGAGGAAAGGNSNLATLAHVAYAQKAQGGARANAGTAPPATHSSSARSGRDYQPVALPRAELKGSIEAYFHEEQQQKQSKGAGSAGSSSLRGPRLNGANPPLEGLAASLQDHVRARKYKEETEERQRRAAAAASSSAGPPAGMELPTHYAHQAPPAHSYHHHGASINGTPHKVELGIKRSSPLAPHQQPPRPSKLAHYEPPTTQQQHAHAHLYANGQVLPPPPAHDATTPSPTPSSSSSSCGRRSNSNNGKLLVDPPLLMSPEINSLLGDERPLQLSHHQQQQQQMLHHHQSQQQQHLQLTQQQLRVAHLGHGLSHGHSTMPTLGGQRNGNGNAADDDDVIAADDETHWQHRVSSGFDRLVAFASTELDKTRRSIDGDTVPASISCNTSPDSGITHSSSNSDAVRTFLSTSSSSSQLELPIGSGGSSCNSLYNHHAQHNNIGIGGSGGSHPHQQQQQLQAQHQQQQHHLSDHMSDSSMKSSASSSLHAGSSLKTVSPVDPSAIESPPLSDVGLPRTPSPSAASVATPPLASGPPVSGDLGGIPLKYQRKSKTSSEKHYKKKFCERNWEYDCDELLAYSNSSAPPTAADAAGNGPLNLDAPNTVGGAPAPLLGKSGKSPKEKSSPHHTGLNHQQQHHHKSSKFRPKGKDWDWSMDSRSQTGELLAASNNMNINNNSATTTSN

Target ORF information:

RefSeq Version	NM_169144.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster grappa (gpp), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169144.2

ORF Insert Sequence:

ATGGCCACAC CCCAAGTCAA GGACTTGGTG TTGCGCTCGC CCGCTGGCTC CTCCGATGTC 
ATTTCCTTCG CCTGGCCCCT GCAGATCGGA CACGGACAGG ATAAGCACGA CAATGGCATC 
GACATCATCG ACACCATTAA GTTCGTCTGC GATGAACTGC CATCGATGTC ATCGGCCTTC 
GAGGAAACCA ATCTCCACCA AATTGACACT GCCTGCTATA AGACTATGAC CGGTCTGGTC 
GATCGCTTCA ACAAGGCCGT CGACAGTATC GTTGCATTGG AAAAAGGAAC TTCACTGCCC 
GCCGAGCGCC TGAACAAGTT CGCTCACCCT AGCCTTCTAA GACACATATT GCAATTAGTT 
TACAATGCCG CCGTACTCGA TCCGGATAAA CTCAACCAGT ACGAGCCCTT CTCGCCAGAG 
GTTTACGGCG AGACGAGCTA TGAGCTGGTG CAGCAGATGC TTAAGCACGT CACTGTCAGC 
AAGGAGGACA CGTTTATCGA TCTCGGCTCG GGGGTGGGCC AGGTGGTTCT GCAGATGGCC 
GGATCGTTTC CCCTGAAAAC CTGCATCGGC ATCGAGAAGG CCGACACGCC GGCGCGATAT 
GCGGAACGCA TGGATGTTAT CTTCCGCCAG TATATGGGAT GGTTTGGAAA ACGCTTTTGC 
GAATACAAGC TGATAAAGGG TGACTTCCTG GTCGATGAGC ATCGTGAAAA CATCACCTCA 
TCGACGCTGG TTTTTGTAAA TAACTTCGCC TTTGGGCCAA CTGTGGACCA TCAGCTCAAG 
GAACGGTTCG CAGATCTTCG CGACGGAGCA CGGGTCGTGT CCTCCAAGTC GTTTTGCCCG 
CTGAACTTTC GGATCACAGA CAGAAACCTC AGTGACATTG GCACCATTAT GCACGTCAGC 
GAAATTCCGC CCCTCAAGGG GTCCGTTTCC TGGACCTGCA AGCCCGTTTC GTATTATCTG 
CATGTGATCG ATCGCACCAT ATTGGAGCGA TACTTTCAGC GCCTGAAAAC TAAGGGCGGC 
AACGATCACG AGAGCGTGGG AACTGTTCGC ACCACACGTG ATCGCGCCAA GCGAGAGGCA 
AACGTTGGCC AACATCATCA CAACAATCAT CATAGCAACA ACCACGCCAA CAGCAACAAC 
CATCAGAGGG ATCGGGAGCA GTCGAACGGA GCTACCGCAA CTGCAGCTCA TCAGCAACGG 
CATCAGTCCC AGTCGCCAGC TAATGTAAGC GGTGCGGGAA TAGTACTTGC AGCTAGCGGG 
CAACAAGCAG CTTCCAAAAC ACGCCAGCAA CTGCAGCATC AGCATAACCA GCAGCAACGC 
AGCTTGGACA TGGAAAGCAG CACGGAAAGC GATGGCGACG CAACGAATGG CAATGGTGGC 
AATACCACCA CTGCCACCAA TACCACCTCC GCCTCCAATG GCCCGATGAC TAGGAAAGTT 
TGGTCCGACT GGTGCAGTTC CAAAGGCAAG AGTTCGCAGT CTGACGACGA GGAGAACAAC 
AACTCGAACA GCAATGGCGG CAGTAATGGT GGTAGCATTG GAGGAGGATC AGTTGGCCGA 
CAGGCTCGGG CTACAACCCA GAAGAAGCGC AAAAAGTTGA CTAGGAAGGC TGCGATAGCC 
AGCAAATCTG CTGCCGCTGC CCAAAGGGAA GCGGAGGCGG CAGCGGCGGC GGCGGTATCC 
GTACCAAGCA AGGAGTCCAG TTCCAAAGAG GATCCACCAA GGGCTGCTAG CGCCGGACCT 
GGTCGCAAAG GACGCATGAA GAAGGGTGCG CGCGGCAGGA AGTCTTTAAA GATTGTTGGA 
CTAGAAGCTC TGCACAAGCA AACAGTGTTG AGTACCTCCT TGGATACCAT GACCAAGAAG 
TTGCCTGCAG CTCCTGGAAC CGTGGATCAA CAGCTTACTG CTTTGCTCAC GGAAAACATG 
TCGCATGCAG AGTTGGATAT TCCAACGGCG CCACAAGACA CGCCTTATGC CTTGCAGATC 
CTTCTAGACG TGTTCCGTTC GCAGTACACA TCGATGATCG AGCACATGAA GTCAAGTGCC 
TATGTGCCTC AAGTTCAGAA GCAGATTGCC CAAGAGCAGG AGCGGATGGC CAGGCTGAAA 
AACAGAGCCA GCCAGTTGGA TAAGCAGATA AAAGTGCTGA TCGATGACAG TGTGGCTTTA 
CTTAAAGTGC GAATGAACGA GCTGGGCATC CATGTAAACT CACCAAACGA TTTGATCGCC 
CAGGCAAAGG AGATTGTGGG TAGACATAAG GATCTACAGC ACACTGCAAG TCGAATGCGA 
AACGAAGTAA CCTTCTATGA GGGCGAACAG AAGCTGCTCC TTAACAAACA ACTAAAAAAT 
CTACCCGAGT ATCAAAAACT CTGTGGTACT GTTAATGGAA AGGTCAAGCT GGAGGTGCCC 
CCGGAACTTT CCGAGACCAC GGCCCAGGAG CTGGTGCTAA AGGAGATCGC CAACACGCTA 
AGTCAACGAA AGAAACTTTA CGCCCAGGTG TCCACCATTG AACAGGAGAC TTCGGTTTTG 
CAGAAGACAG CAGAGGAAAG GTCCACGGCA GCGACGCTCT TGGCACAAGG AACAAATATG 
ATTGTGTCGA CCGGATCATC TTCCTCCTCC TCTACCACTG TTTGTGCAAG TGCAGTTACG 
GCGCAGAGCA ACAAGTTGAA CTCCGTGAAG AACTCTCGTC GCAATCGCGA GCACCGAGCG 
CGTTCCCAGG AGTGGCCAGA GGTTCCCGAA GTGGGTAAAA TCCAGGAAAG CAATCCCGAA 
GTGCTGGCAC AGAAAATAGT CGAGACCTGC CGCCAAATCG AAGCTGGCAA GTTCCAAGGA 
GCAGGTGCGC CCTCATCGCA AGTGAATGGC AAGAATAAAG CAATTATAGA GGTCCCACCA 
CCTCCGGCCA CTGCTCCAGT GAGCATTAAA TCCTCGCCTG GGCACCACTA TAAAGACACA 
ACACTGATGC CAGCACCCAA ACAACAACAG CAACAACAGA TGACGCTTTC CCAGCTTCCG 
AAGTGTGAGC TGCCCGGACT ATCGACTAGT CGCAAACAGG AATCTCCCAA GGTTGCCAAC 
TTTGAGGATC GGTTAAAGAG TATCATCACC ACGGCCTTAA ACGAAGATCA GGAGCAGCGC 
AGCAAGGCAG TGGAGTCGTC ACCCTCTCCC TCACCTCTTC ACAGTCCAGC ACCCAAGCGA 
TCAAAGCAGC ATCCGGCTGG AGCGATAAAC CCTGCCCAGT CGCTGCCCAA CAATCTGCAC 
AACATCATCA CCGTCTCCAC GCAGGGTTTG ATGCACCTTA ATGCCAATAC AACAATATCC 
CCAATAACAC CACCACTGCC GGGTCCAGGT GCAGGAGCAA CCGCATCCAC TGCGCCGCCT 
CCCCCAGCGA ATCTTCCTTA TGGGGCTTAC GGCGGGGCAG TGGCAAAGAC AACGATATCG 
GGGAAGTACC AGGCAGCTAA GGAACCGAAG TATTCGCCAG TTAGACAGGC ACCATTACCT 
CCTCCCCCCT CCCACATGGC ATCTTTGTAT CCAGCTGGAC AGCAGACTAC TCCGGCGGAC 
CTAGGTTACC AGCGGCGACG CAGCTCTGTT AGTGCCACTA GCTACGAACA CTATATGGTA 
CAGCAGCAGC AGCAACTCCA GCAACAACAA CTCATGCTAG CAGCCGCTGC TCATGCGGCC 
CAGCGTCAAC AGATGCGCGT AGAAGAACAA CAGCAGCAGC AGCAGCACCA ACACCACCAC 
CACCACCACC ACCACCATCC CCAACACCGT CTGCCGCAGC ATGTGCAGCA TCAGCATCCG 
CATCAGCATC ATCCCAATGA GTTCAAGGCA CCGCCGGCTG ATAGTCATCT CCAGCGATCG 
AGCAGTCGTG AGCAATTAAT AGTGGAGCCC CCGCAGACGC AACCGCTGGA GCTGCTGCCT 
CGAGCAAGTT CCGCCAATTC GGATTACAGC GGATATCGCA TCCGTCCGCC AAGCAGGCCA 
AGCTCCAACT CCTCGCAACC AGATTACACG CAAGTTTCAC CCGCAAAGAT GGCTCTGCGG 
CGGCATCTGT CGCAGGAGAA GCTGAGCCAA CATGTCACGC CCCAGGCCAC ACCGCCGCTT 
CCTGGTCACG GAGGAGCACC CACATCAGGG AAGACCATTG GCGATTTGGT TAACGGGGAA 
ATCGAGCGAA CGCTTGAGAT TTCGCATCAG AGCATCATTA ATGCGGCTGT GAATATGAGC 
ACTAGTGGCG CTAGCTTCAT GGAGCGGGCC TTCCTTAATG AGCGCTCCAA CGATCGTTTG 
CTGATCAACT TGAATGCCCA ACGACCAGAG AGAGTTCATG TTCGTCCGCT CTCCGAAGAA 
TCGCAGGATC CACAGCCCAC CAGTTATGCT CAGGAGAGAG GACCTGGTCT AGGTGCTGGT 
GGAGCAGCAG CTGGAGGTAA CAGCAATCTG GCCACTTTGG CACATGTAGC CTACGCCCAA 
AAGGCACAGG GCGGCGCTCG AGCCAACGCA GGAACGGCAC CACCCGCAAC CCACTCCTCC 
TCTGCTCGTT CTGGACGTGA CTATCAGCCT GTGGCTTTGC CCCGTGCTGA GCTTAAGGGC 
AGCATTGAGG CCTATTTCCA CGAGGAGCAG CAGCAGAAGC AGTCGAAGGG CGCAGGATCA 
GCAGGATCGT CCTCTTTGCG GGGACCGCGT CTTAATGGTG CCAATCCTCC ATTGGAAGGT 
CTAGCAGCAT CGCTGCAGGA TCATGTCCGT GCCAGAAAGT ACAAGGAGGA GACCGAGGAG 
CGTCAACGAC GTGCAGCAGC CGCTGCCTCG TCTTCGGCGG GACCGCCTGC TGGCATGGAA 
CTGCCAACGC ACTATGCTCA TCAGGCGCCT CCAGCCCACA GCTACCACCA CCATGGTGCA 
AGCATCAATG GAACACCGCA CAAAGTAGAA CTGGGAATAA AACGCAGCTC ACCGCTGGCG 
CCGCATCAGC AACCGCCACG TCCCTCGAAA TTGGCACACT ACGAGCCGCC AACGACGCAG 
CAGCAGCATG CCCATGCCCA CCTGTACGCC AACGGACAAG TGCTACCTCC GCCTCCAGCT 
CATGATGCGA CCACGCCCTC CCCGACGCCC TCGTCATCTT CATCGTCCTG CGGACGTCGA 
AGCAACAGCA ACAATGGAAA GTTGCTCGTG GATCCACCTT TGCTAATGAG TCCCGAGATC 
AATTCACTGT TGGGCGACGA AAGGCCTTTG CAGCTGTCGC ACCACCAACA GCAGCAGCAG 
CAAATGCTGC ATCACCATCA ATCGCAGCAA CAGCAGCATC TGCAGCTGAC CCAACAGCAA 
CTGCGTGTAG CTCATCTTGG ACATGGTCTT AGTCACGGTC ACAGCACCAT GCCCACTTTG 
GGCGGACAAC GCAATGGCAA TGGCAATGCC GCCGACGATG ACGATGTTAT TGCCGCTGAT 
GATGAGACCC ACTGGCAGCA TCGAGTCAGC TCTGGTTTCG ACCGCCTCGT GGCCTTCGCC 
AGCACTGAAT TGGATAAGAC CAGGCGAAGC ATCGATGGCG ATACCGTGCC GGCCTCGATT 
AGTTGCAACA CGTCTCCGGA CTCGGGCATT ACGCACAGCA GCAGCAACTC AGATGCAGTG 
CGCACATTTT TGTCCACCTC GTCCAGCTCC TCGCAGCTGG AGCTGCCCAT AGGAAGCGGT 
GGTAGTAGCT GCAACAGCCT CTACAACCAT CATGCCCAGC ACAACAACAT TGGCATTGGC 
GGCAGCGGAG GCAGCCACCC ACATCAGCAA CAGCAACAGT TGCAGGCGCA GCACCAACAG 
CAGCAGCACC ATCTTTCCGA TCACATGAGC GACAGTAGCA TGAAATCCTC GGCATCCTCC 
TCGCTGCATG CTGGTAGCTC CCTTAAGACC GTGTCGCCAG TGGATCCCAG TGCCATCGAG 
TCGCCGCCAC TCTCGGACGT TGGATTGCCC AGAACTCCCA GTCCCAGTGC AGCCAGTGTG 
GCCACTCCAC CACTGGCCAG TGGACCACCC GTGTCCGGGG ACTTAGGTGG TATACCTCTG 
AAGTACCAGC GCAAGTCCAA GACCAGTTCG GAAAAGCACT ACAAAAAGAA GTTCTGCGAA 
AGAAACTGGG AGTACGACTG TGATGAACTG CTGGCCTACT CCAACTCCAG TGCACCGCCG 
ACGGCAGCAG ATGCAGCGGG CAATGGGCCA CTTAACTTGG ATGCTCCCAA TACTGTGGGC 
GGAGCACCTG CACCGCTCTT GGGAAAATCG GGAAAGTCAC CCAAGGAAAA GTCTTCACCG 
CACCACACGG GCTTAAACCA TCAGCAGCAG CACCATCACA AGTCATCCAA ATTTCGGCCA 
AAGGGCAAGG ACTGGGACTG GTCAATGGAC AGTCGGAGCC AAACGGGCGA GCTGCTGGCC 
GCCAGTAACA ACATGAACAT TAACAACAAC TCAGCCACCA CAACGAGTAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gpp ( NM_001275387.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262316.1 Drosophila melanogaster grappa (gpp), transcript variant F, mRNA.

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CloneID	OFa07560
Clone ID Related Accession (Same CDS sequence)	NM_001275387.1
Accession Version	NM_001275387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5697bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	grappa, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACAC CCCAAGTCAA GGACTTGGTG TTGCGCTCGC CCGCTGGCTC CTCCGATGTC 
ATTTCCTTCG CCTGGCCCCT GCAGATCGGA CACGGACAGG ATAAGCACGA CAATGGCATC 
GACATCATCG ACACCATTAA GTTCGTCTGC GATGAACTGC CATCGATGTC ATCGGCCTTC 
GAGGAAACCA ATCTCCACCA AATTGACACT GCCTGCTATA AGACTATGAC CGGTCTGGTC 
GATCGCTTCA ACAAGGCCGT CGACAGTATC GTTGCATTGG AAAAAGGAAC TTCACTGCCC 
GCCGAGCGCC TGAACAAGTT CGCTCACCCT AGCCTTCTAA GACACATATT GCAATTAGTT 
TACAATGCCG CCGTACTCGA TCCGGATAAA CTCAACCAGT ACGAGCCCTT CTCGCCAGAG 
GTTTACGGCG AGACGAGCTA TGAGCTGGTG CAGCAGATGC TTAAGCACGT CACTGTCAGC 
AAGGAGGACA CGTTTATCGA TCTCGGCTCG GGGGTGGGCC AGGTGGTTCT GCAGATGGCC 
GGATCGTTTC CCCTGAAAAC CTGCATCGGC ATCGAGAAGG CCGACACGCC GGCGCGATAT 
GCGGAACGCA TGGATGTTAT CTTCCGCCAG TATATGGGAT GGTTTGGAAA ACGCTTTTGC 
GAATACAAGC TGATAAAGGG TGACTTCCTG GTCGATGAGC ATCGTGAAAA CATCACCTCA 
TCGACGCTGG TTTTTGTAAA TAACTTCGCC TTTGGGCCAA CTGTGGACCA TCAGCTCAAG 
GAACGGTTCG CAGATCTTCG CGACGGAGCA CGGGTCGTGT CCTCCAAGTC GTTTTGCCCG 
CTGAACTTTC GGATCACAGA CAGAAACCTC AGTGACATTG GCACCATTAT GCACGTCAGC 
GAAATTCCGC CCCTCAAGGG GTCCGTTTCC TGGACCTGCA AGCCCGTTTC GTATTATCTG 
CATGTGATCG ATCGCACCAT ATTGGAGCGA TACTTTCAGC GCCTGAAAAC TAAGGGCGGC 
AACGATCACG AGAGCGTGGG AACTGTTCGC ACCACACGTG ATCGCGCCAA GCGAGAGGCA 
AACGTTGGCC AACATCATCA CAACAATCAT CATAGCAACA ACCACGCCAA CAGCAACAAC 
CATCAGAGGG ATCGGGAGCA GTCGAACGGA GCTACCGCAA CTGCAGCTCA TCAGCAACGG 
CATCAGTCCC AGTCGCCAGC TAATGTAAGC GGTGCGGGAA TAGTACTTGC AGCTAGCGGG 
CAACAAGCAG CTTCCAAAAC ACGCCAGCAA CTGCAGCATC AGCATAACCA GCAGCAACGC 
AGCTTGGACA TGGAAAGCAG CACGGAAAGC GATGGCGACG CAACGAATGG CAATGGTGGC 
AATACCACCA CTGCCACCAA TACCACCTCC GCCTCCAATG GCCCGATGAC TAGGAAAGTT 
TGGTCCGACT GGTGCAGTTC CAAAGGCAAG AGTTCGCAGT CTGACGACGA GGAGAACAAC 
AACTCGAACA GCAATGGCGG CAGTAATGGT GGTAGCATTG GAGGAGGATC AGTTGGCCGA 
CAGGCTCGGG CTACAACCCA GAAGAAGCGC AAAAAGTTGA CTAGGAAGGC TGCGATAGCC 
AGCAAATCTG CTGCCGCTGC CCAAAGGGAA GCGGAGGCGG CAGCGGCGGC GGCGGTATCC 
GTACCAAGCA AGGAGTCCAG TTCCAAAGAG GATCCACCAA GGGCTGCTAG CGCCGGACCT 
GGTCGCAAAG GACGCATGAA GAAGGGTGCG CGCGGCAGGA AGTCTTTAAA GATTGTTGGA 
CTAGAAGCTC TGCACAAGCA AACAGTGTTG AGTACCTCCT TGGATACCAT GACCAAGAAG 
TTGCCTGCAG CTCCTGGAAC CGTGGATCAA CAGCTTACTG CTTTGCTCAC GGAAAACATG 
TCGCATGCAG AGTTGGATAT TCCAACGGCG CCACAAGACA CGCCTTATGC CTTGCAGATC 
CTTCTAGACG TGTTCCGTTC GCAGTACACA TCGATGATCG AGCACATGAA GTCAAGTGCC 
TATGTGCCTC AAGTTCAGAA GCAGATTGCC CAAGAGCAGG AGCGGATGGC CAGGCTGAAA 
AACAGAGCCA GCCAGTTGGA TAAGCAGATA AAAGTGCTGA TCGATGACAG TGTGGCTTTA 
CTTAAAGTGC GAATGAACGA GCTGGGCATC CATGTAAACT CACCAAACGA TTTGATCGCC 
CAGGCAAAGG AGATTGTGGG TAGACATAAG GATCTACAGC ACACTGCAAG TCGAATGCGA 
AACGAAGTAA CCTTCTATGA GGGCGAACAG AAGCTGCTCC TTAACAAACA ACTAAAAAAT 
CTACCCGAGT ATCAAAAACT CTGTGGTACT GTTAATGGAA AGGTCAAGCT GGAGGTGCCC 
CCGGAACTTT CCGAGACCAC GGCCCAGGAG CTGGTGCTAA AGGAGATCGC CAACACGCTA 
AGTCAACGAA AGAAACTTTA CGCCCAGGTG TCCACCATTG AACAGGAGAC TTCGGTTTTG 
CAGAAGACAG CAGAGGAAAG GTCCACGGCA GCGACGCTCT TGGCACAAGG AACAAATATG 
ATTGTGTCGA CCGGATCATC TTCCTCCTCC TCTACCACTG TTTGTGCAAG TGCAGTTACG 
GCGCAGAGCA ACAAGTTGAA CTCCGTGAAG AACTCTCGTC GCAATCGCGA GCACCGAGCG 
CGTTCCCAGG AGTGGCCAGA GGTTCCCGAA GTGGGTAAAA TCCAGGAAAG CAATCCCGAA 
GTGCTGGCAC AGAAAATAGT CGAGACCTGC CGCCAAATCG AAGCTGGCAA GTTCCAAGGA 
GCAGGTGCGC CCTCATCGCA AGTGAATGGC AAGAATAAAG CAATTATAGA GGTCCCACCA 
CCTCCGGCCA CTGCTCCAGT GAGCATTAAA TCCTCGCCTG GGCACCACTA TAAAGACACA 
ACACTGATGC CAGCACCCAA ACAACAACAG CAACAACAGA TGACGCTTTC CCAGCTTCCG 
AAGTGTGAGC TGCCCGGACT ATCGACTAGT CGCAAACAGG AATCTCCCAA GGTTGCCAAC 
TTTGAGGATC GGTTAAAGAG TATCATCACC ACGGCCTTAA ACGAAGATCA GGAGCAGCGC 
AGCAAGGCAG TGGAGTCGTC ACCCTCTCCC TCACCTCTTC ACAGTCCAGC ACCCAAGCGA 
TCAAAGCAGC ATCCGGCTGG AGCGATAAAC CCTGCCCAGT CGCTGCCCAA CAATCTGCAC 
AACATCATCA CCGTCTCCAC GCAGGGTTTG ATGCACCTTA ATGCCAATAC AACAATATCC 
CCAATAACAC CACCACTGCC GGGTCCAGGT GCAGGAGCAA CCGCATCCAC TGCGCCGCCT 
CCCCCAGCGA ATCTTCCTTA TGGGGCTTAC GGCGGGGCAG TGGCAAAGAC AACGATATCG 
GGGAAGTACC AGGCAGCTAA GGAACCGAAG TATTCGCCAG TTAGACAGGC ACCATTACCT 
CCTCCCCCCT CCCACATGGC ATCTTTGTAT CCAGCTGGAC AGCAGACTAC TCCGGCGGAC 
CTAGGTTACC AGCGGCGACG CAGCTCTGTT AGTGCCACTA GCTACGAACA CTATATGGTA 
CAGCAGCAGC AGCAACTCCA GCAACAACAA CTCATGCTAG CAGCCGCTGC TCATGCGGCC 
CAGCGTCAAC AGATGCGCGT AGAAGAACAA CAGCAGCAGC AGCAGCACCA ACACCACCAC 
CACCACCACC ACCACCATCC CCAACACCGT CTGCCGCAGC ATGTGCAGCA TCAGCATCCG 
CATCAGCATC ATCCCAATGA GTTCAAGGCA CCGCCGGCTG ATAGTCATCT CCAGCGATCG 
AGCAGTCGTG AGCAATTAAT AGTGGAGCCC CCGCAGACGC AACCGCTGGA GCTGCTGCCT 
CGAGCAAGTT CCGCCAATTC GGATTACAGC GGATATCGCA TCCGTCCGCC AAGCAGGCCA 
AGCTCCAACT CCTCGCAACC AGATTACACG CAAGTTTCAC CCGCAAAGAT GGCTCTGCGG 
CGGCATCTGT CGCAGGAGAA GCTGAGCCAA CATGTCACGC CCCAGGCCAC ACCGCCGCTT 
CCTGGTCACG GAGGAGCACC CACATCAGGG AAGACCATTG GCGATTTGGT TAACGGGGAA 
ATCGAGCGAA CGCTTGAGAT TTCGCATCAG AGCATCATTA ATGCGGCTGT GAATATGAGC 
ACTAGTGGCG CTAGCTTCAT GGAGCGGGCC TTCCTTAATG AGCGCTCCAA CGATCGTTTG 
CTGATCAACT TGAATGCCCA ACGACCAGAG AGAGTTCATG TTCGTCCGCT CTCCGAAGAA 
TCGCAGGATC CACAGCCCAC CAGTTATGCT CAGGAGAGAG GACCTGGTCT AGGTGCTGGT 
GGAGCAGCAG CTGGAGGTAA CAGCAATCTG GCCACTTTGG CACATGTAGC CTACGCCCAA 
AAGGCACAGG GCGGCGCTCG AGCCAACGCA GGAACGGCAC CACCCGCAAC CCACTCCTCC 
TCTGCTCGTT CTGGACGTGA CTATCAGCCT GTGGCTTTGC CCCGTGCTGA GCTTAAGGGC 
AGCATTGAGG CCTATTTCCA CGAGGAGCAG CAGCAGAAGC AGTCGAAGGG CGCAGGATCA 
GCAGGATCGT CCTCTTTGCG GGGACCGCGT CTTAATGGTG CCAATCCTCC ATTGGAAGGT 
CTAGCAGCAT CGCTGCAGGA TCATGTCCGT GCCAGAAAGT ACAAGGAGGA GACCGAGGAG 
CGTCAACGAC GTGCAGCAGC CGCTGCCTCG TCTTCGGCGG GACCGCCTGC TGGCATGGAA 
CTGCCAACGC ACTATGCTCA TCAGGCGCCT CCAGCCCACA GCTACCACCA CCATGGTGCA 
AGCATCAATG GAACACCGCA CAAAGTAGAA CTGGGAATAA AACGCAGCTC ACCGCTGGCG 
CCGCATCAGC AACCGCCACG TCCCTCGAAA TTGGCACACT ACGAGCCGCC AACGACGCAG 
CAGCAGCATG CCCATGCCCA CCTGTACGCC AACGGACAAG TGCTACCTCC GCCTCCAGCT 
CATGATGCGA CCACGCCCTC CCCGACGCCC TCGTCATCTT CATCGTCCTG CGGACGTCGA 
AGCAACAGCA ACAATGGAAA GTTGCTCGTG GATCCACCTT TGCTAATGAG TCCCGAGATC 
AATTCACTGT TGGGCGACGA AAGGCCTTTG CAGCTGTCGC ACCACCAACA GCAGCAGCAG 
CAAATGCTGC ATCACCATCA ATCGCAGCAA CAGCAGCATC TGCAGCTGAC CCAACAGCAA 
CTGCGTGTAG CTCATCTTGG ACATGGTCTT AGTCACGGTC ACAGCACCAT GCCCACTTTG 
GGCGGACAAC GCAATGGCAA TGGCAATGCC GCCGACGATG GTAAGCTATA CGTAGACCAA 
GCAAAAAACT CGACCAGGGT ATCTGTACCC AAATATGCAC CACAACAAGC ACCACCACCT 
GCACACGTAG CATTCCCGCT AGAGCCACAT ATTCTTCACC AGCACAAATA CCAACGACTA 
GCCACCCAAA CGGTGGCAGA AGATCTTACC ATGAAGCGAA AAGAGCAGGA ATCGGATTCA 
GAACTCGAGC CGGAAGTGGT TGCTGCGTTT GTCGAAGATG GAGGTTCCCA TAGCGGATCC 
TCATCGTCGA GTTCCAGCTC GCACTCAGGA TCTGATTCCA GTTCTGACGT GCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262316.1
CDS	1852..7548
Translation	MATPQVKDLVLRSPAGSSDVISFAWPLQIGHGQDKHDNGIDIIDTIKFVCDELPSMSSAFEETNLHQIDTACYKTMTGLVDRFNKAVDSIVALEKGTSLPAERLNKFAHPSLLRHILQLVYNAAVLDPDKLNQYEPFSPEVYGETSYELVQQMLKHVTVSKEDTFIDLGSGVGQVVLQMAGSFPLKTCIGIEKADTPARYAERMDVIFRQYMGWFGKRFCEYKLIKGDFLVDEHRENITSSTLVFVNNFAFGPTVDHQLKERFADLRDGARVVSSKSFCPLNFRITDRNLSDIGTIMHVSEIPPLKGSVSWTCKPVSYYLHVIDRTILERYFQRLKTKGGNDHESVGTVRTTRDRAKREANVGQHHHNNHHSNNHANSNNHQRDREQSNGATATAAHQQRHQSQSPANVSGAGIVLAASGQQAASKTRQQLQHQHNQQQRSLDMESSTESDGDATNGNGGNTTTATNTTSASNGPMTRKVWSDWCSSKGKSSQSDDEENNNSNSNGGSNGGSIGGGSVGRQARATTQKKRKKLTRKAAIASKSAAAAQREAEAAAAAAVSVPSKESSSKEDPPRAASAGPGRKGRMKKGARGRKSLKIVGLEALHKQTVLSTSLDTMTKKLPAAPGTVDQQLTALLTENMSHAELDIPTAPQDTPYALQILLDVFRSQYTSMIEHMKSSAYVPQVQKQIAQEQERMARLKNRASQLDKQIKVLIDDSVALLKVRMNELGIHVNSPNDLIAQAKEIVGRHKDLQHTASRMRNEVTFYEGEQKLLLNKQLKNLPEYQKLCGTVNGKVKLEVPPELSETTAQELVLKEIANTLSQRKKLYAQVSTIEQETSVLQKTAEERSTAATLLAQGTNMIVSTGSSSSSSTTVCASAVTAQSNKLNSVKNSRRNREHRARSQEWPEVPEVGKIQESNPEVLAQKIVETCRQIEAGKFQGAGAPSSQVNGKNKAIIEVPPPPATAPVSIKSSPGHHYKDTTLMPAPKQQQQQQMTLSQLPKCELPGLSTSRKQESPKVANFEDRLKSIITTALNEDQEQRSKAVESSPSPSPLHSPAPKRSKQHPAGAINPAQSLPNNLHNIITVSTQGLMHLNANTTISPITPPLPGPGAGATASTAPPPPANLPYGAYGGAVAKTTISGKYQAAKEPKYSPVRQAPLPPPPSHMASLYPAGQQTTPADLGYQRRRSSVSATSYEHYMVQQQQQLQQQQLMLAAAAHAAQRQQMRVEEQQQQQQHQHHHHHHHHHPQHRLPQHVQHQHPHQHHPNEFKAPPADSHLQRSSSREQLIVEPPQTQPLELLPRASSANSDYSGYRIRPPSRPSSNSSQPDYTQVSPAKMALRRHLSQEKLSQHVTPQATPPLPGHGGAPTSGKTIGDLVNGEIERTLEISHQSIINAAVNMSTSGASFMERAFLNERSNDRLLINLNAQRPERVHVRPLSEESQDPQPTSYAQERGPGLGAGGAAAGGNSNLATLAHVAYAQKAQGGARANAGTAPPATHSSSARSGRDYQPVALPRAELKGSIEAYFHEEQQQKQSKGAGSAGSSSLRGPRLNGANPPLEGLAASLQDHVRARKYKEETEERQRRAAAAASSSAGPPAGMELPTHYAHQAPPAHSYHHHGASINGTPHKVELGIKRSSPLAPHQQPPRPSKLAHYEPPTTQQQHAHAHLYANGQVLPPPPAHDATTPSPTPSSSSSSCGRRSNSNNGKLLVDPPLLMSPEINSLLGDERPLQLSHHQQQQQQMLHHHQSQQQQHLQLTQQQLRVAHLGHGLSHGHSTMPTLGGQRNGNGNAADDGKLYVDQAKNSTRVSVPKYAPQQAPPPAHVAFPLEPHILHQHKYQRLATQTVAEDLTMKRKEQESDSELEPEVVAAFVEDGGSHSGSSSSSSSSHSGSDSSSDVP

Target ORF information:

RefSeq Version	NM_001275387.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster grappa (gpp), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275387.1

ORF Insert Sequence:

ATGGCCACAC CCCAAGTCAA GGACTTGGTG TTGCGCTCGC CCGCTGGCTC CTCCGATGTC 
ATTTCCTTCG CCTGGCCCCT GCAGATCGGA CACGGACAGG ATAAGCACGA CAATGGCATC 
GACATCATCG ACACCATTAA GTTCGTCTGC GATGAACTGC CATCGATGTC ATCGGCCTTC 
GAGGAAACCA ATCTCCACCA AATTGACACT GCCTGCTATA AGACTATGAC CGGTCTGGTC 
GATCGCTTCA ACAAGGCCGT CGACAGTATC GTTGCATTGG AAAAAGGAAC TTCACTGCCC 
GCCGAGCGCC TGAACAAGTT CGCTCACCCT AGCCTTCTAA GACACATATT GCAATTAGTT 
TACAATGCCG CCGTACTCGA TCCGGATAAA CTCAACCAGT ACGAGCCCTT CTCGCCAGAG 
GTTTACGGCG AGACGAGCTA TGAGCTGGTG CAGCAGATGC TTAAGCACGT CACTGTCAGC 
AAGGAGGACA CGTTTATCGA TCTCGGCTCG GGGGTGGGCC AGGTGGTTCT GCAGATGGCC 
GGATCGTTTC CCCTGAAAAC CTGCATCGGC ATCGAGAAGG CCGACACGCC GGCGCGATAT 
GCGGAACGCA TGGATGTTAT CTTCCGCCAG TATATGGGAT GGTTTGGAAA ACGCTTTTGC 
GAATACAAGC TGATAAAGGG TGACTTCCTG GTCGATGAGC ATCGTGAAAA CATCACCTCA 
TCGACGCTGG TTTTTGTAAA TAACTTCGCC TTTGGGCCAA CTGTGGACCA TCAGCTCAAG 
GAACGGTTCG CAGATCTTCG CGACGGAGCA CGGGTCGTGT CCTCCAAGTC GTTTTGCCCG 
CTGAACTTTC GGATCACAGA CAGAAACCTC AGTGACATTG GCACCATTAT GCACGTCAGC 
GAAATTCCGC CCCTCAAGGG GTCCGTTTCC TGGACCTGCA AGCCCGTTTC GTATTATCTG 
CATGTGATCG ATCGCACCAT ATTGGAGCGA TACTTTCAGC GCCTGAAAAC TAAGGGCGGC 
AACGATCACG AGAGCGTGGG AACTGTTCGC ACCACACGTG ATCGCGCCAA GCGAGAGGCA 
AACGTTGGCC AACATCATCA CAACAATCAT CATAGCAACA ACCACGCCAA CAGCAACAAC 
CATCAGAGGG ATCGGGAGCA GTCGAACGGA GCTACCGCAA CTGCAGCTCA TCAGCAACGG 
CATCAGTCCC AGTCGCCAGC TAATGTAAGC GGTGCGGGAA TAGTACTTGC AGCTAGCGGG 
CAACAAGCAG CTTCCAAAAC ACGCCAGCAA CTGCAGCATC AGCATAACCA GCAGCAACGC 
AGCTTGGACA TGGAAAGCAG CACGGAAAGC GATGGCGACG CAACGAATGG CAATGGTGGC 
AATACCACCA CTGCCACCAA TACCACCTCC GCCTCCAATG GCCCGATGAC TAGGAAAGTT 
TGGTCCGACT GGTGCAGTTC CAAAGGCAAG AGTTCGCAGT CTGACGACGA GGAGAACAAC 
AACTCGAACA GCAATGGCGG CAGTAATGGT GGTAGCATTG GAGGAGGATC AGTTGGCCGA 
CAGGCTCGGG CTACAACCCA GAAGAAGCGC AAAAAGTTGA CTAGGAAGGC TGCGATAGCC 
AGCAAATCTG CTGCCGCTGC CCAAAGGGAA GCGGAGGCGG CAGCGGCGGC GGCGGTATCC 
GTACCAAGCA AGGAGTCCAG TTCCAAAGAG GATCCACCAA GGGCTGCTAG CGCCGGACCT 
GGTCGCAAAG GACGCATGAA GAAGGGTGCG CGCGGCAGGA AGTCTTTAAA GATTGTTGGA 
CTAGAAGCTC TGCACAAGCA AACAGTGTTG AGTACCTCCT TGGATACCAT GACCAAGAAG 
TTGCCTGCAG CTCCTGGAAC CGTGGATCAA CAGCTTACTG CTTTGCTCAC GGAAAACATG 
TCGCATGCAG AGTTGGATAT TCCAACGGCG CCACAAGACA CGCCTTATGC CTTGCAGATC 
CTTCTAGACG TGTTCCGTTC GCAGTACACA TCGATGATCG AGCACATGAA GTCAAGTGCC 
TATGTGCCTC AAGTTCAGAA GCAGATTGCC CAAGAGCAGG AGCGGATGGC CAGGCTGAAA 
AACAGAGCCA GCCAGTTGGA TAAGCAGATA AAAGTGCTGA TCGATGACAG TGTGGCTTTA 
CTTAAAGTGC GAATGAACGA GCTGGGCATC CATGTAAACT CACCAAACGA TTTGATCGCC 
CAGGCAAAGG AGATTGTGGG TAGACATAAG GATCTACAGC ACACTGCAAG TCGAATGCGA 
AACGAAGTAA CCTTCTATGA GGGCGAACAG AAGCTGCTCC TTAACAAACA ACTAAAAAAT 
CTACCCGAGT ATCAAAAACT CTGTGGTACT GTTAATGGAA AGGTCAAGCT GGAGGTGCCC 
CCGGAACTTT CCGAGACCAC GGCCCAGGAG CTGGTGCTAA AGGAGATCGC CAACACGCTA 
AGTCAACGAA AGAAACTTTA CGCCCAGGTG TCCACCATTG AACAGGAGAC TTCGGTTTTG 
CAGAAGACAG CAGAGGAAAG GTCCACGGCA GCGACGCTCT TGGCACAAGG AACAAATATG 
ATTGTGTCGA CCGGATCATC TTCCTCCTCC TCTACCACTG TTTGTGCAAG TGCAGTTACG 
GCGCAGAGCA ACAAGTTGAA CTCCGTGAAG AACTCTCGTC GCAATCGCGA GCACCGAGCG 
CGTTCCCAGG AGTGGCCAGA GGTTCCCGAA GTGGGTAAAA TCCAGGAAAG CAATCCCGAA 
GTGCTGGCAC AGAAAATAGT CGAGACCTGC CGCCAAATCG AAGCTGGCAA GTTCCAAGGA 
GCAGGTGCGC CCTCATCGCA AGTGAATGGC AAGAATAAAG CAATTATAGA GGTCCCACCA 
CCTCCGGCCA CTGCTCCAGT GAGCATTAAA TCCTCGCCTG GGCACCACTA TAAAGACACA 
ACACTGATGC CAGCACCCAA ACAACAACAG CAACAACAGA TGACGCTTTC CCAGCTTCCG 
AAGTGTGAGC TGCCCGGACT ATCGACTAGT CGCAAACAGG AATCTCCCAA GGTTGCCAAC 
TTTGAGGATC GGTTAAAGAG TATCATCACC ACGGCCTTAA ACGAAGATCA GGAGCAGCGC 
AGCAAGGCAG TGGAGTCGTC ACCCTCTCCC TCACCTCTTC ACAGTCCAGC ACCCAAGCGA 
TCAAAGCAGC ATCCGGCTGG AGCGATAAAC CCTGCCCAGT CGCTGCCCAA CAATCTGCAC 
AACATCATCA CCGTCTCCAC GCAGGGTTTG ATGCACCTTA ATGCCAATAC AACAATATCC 
CCAATAACAC CACCACTGCC GGGTCCAGGT GCAGGAGCAA CCGCATCCAC TGCGCCGCCT 
CCCCCAGCGA ATCTTCCTTA TGGGGCTTAC GGCGGGGCAG TGGCAAAGAC AACGATATCG 
GGGAAGTACC AGGCAGCTAA GGAACCGAAG TATTCGCCAG TTAGACAGGC ACCATTACCT 
CCTCCCCCCT CCCACATGGC ATCTTTGTAT CCAGCTGGAC AGCAGACTAC TCCGGCGGAC 
CTAGGTTACC AGCGGCGACG CAGCTCTGTT AGTGCCACTA GCTACGAACA CTATATGGTA 
CAGCAGCAGC AGCAACTCCA GCAACAACAA CTCATGCTAG CAGCCGCTGC TCATGCGGCC 
CAGCGTCAAC AGATGCGCGT AGAAGAACAA CAGCAGCAGC AGCAGCACCA ACACCACCAC 
CACCACCACC ACCACCATCC CCAACACCGT CTGCCGCAGC ATGTGCAGCA TCAGCATCCG 
CATCAGCATC ATCCCAATGA GTTCAAGGCA CCGCCGGCTG ATAGTCATCT CCAGCGATCG 
AGCAGTCGTG AGCAATTAAT AGTGGAGCCC CCGCAGACGC AACCGCTGGA GCTGCTGCCT 
CGAGCAAGTT CCGCCAATTC GGATTACAGC GGATATCGCA TCCGTCCGCC AAGCAGGCCA 
AGCTCCAACT CCTCGCAACC AGATTACACG CAAGTTTCAC CCGCAAAGAT GGCTCTGCGG 
CGGCATCTGT CGCAGGAGAA GCTGAGCCAA CATGTCACGC CCCAGGCCAC ACCGCCGCTT 
CCTGGTCACG GAGGAGCACC CACATCAGGG AAGACCATTG GCGATTTGGT TAACGGGGAA 
ATCGAGCGAA CGCTTGAGAT TTCGCATCAG AGCATCATTA ATGCGGCTGT GAATATGAGC 
ACTAGTGGCG CTAGCTTCAT GGAGCGGGCC TTCCTTAATG AGCGCTCCAA CGATCGTTTG 
CTGATCAACT TGAATGCCCA ACGACCAGAG AGAGTTCATG TTCGTCCGCT CTCCGAAGAA 
TCGCAGGATC CACAGCCCAC CAGTTATGCT CAGGAGAGAG GACCTGGTCT AGGTGCTGGT 
GGAGCAGCAG CTGGAGGTAA CAGCAATCTG GCCACTTTGG CACATGTAGC CTACGCCCAA 
AAGGCACAGG GCGGCGCTCG AGCCAACGCA GGAACGGCAC CACCCGCAAC CCACTCCTCC 
TCTGCTCGTT CTGGACGTGA CTATCAGCCT GTGGCTTTGC CCCGTGCTGA GCTTAAGGGC 
AGCATTGAGG CCTATTTCCA CGAGGAGCAG CAGCAGAAGC AGTCGAAGGG CGCAGGATCA 
GCAGGATCGT CCTCTTTGCG GGGACCGCGT CTTAATGGTG CCAATCCTCC ATTGGAAGGT 
CTAGCAGCAT CGCTGCAGGA TCATGTCCGT GCCAGAAAGT ACAAGGAGGA GACCGAGGAG 
CGTCAACGAC GTGCAGCAGC CGCTGCCTCG TCTTCGGCGG GACCGCCTGC TGGCATGGAA 
CTGCCAACGC ACTATGCTCA TCAGGCGCCT CCAGCCCACA GCTACCACCA CCATGGTGCA 
AGCATCAATG GAACACCGCA CAAAGTAGAA CTGGGAATAA AACGCAGCTC ACCGCTGGCG 
CCGCATCAGC AACCGCCACG TCCCTCGAAA TTGGCACACT ACGAGCCGCC AACGACGCAG 
CAGCAGCATG CCCATGCCCA CCTGTACGCC AACGGACAAG TGCTACCTCC GCCTCCAGCT 
CATGATGCGA CCACGCCCTC CCCGACGCCC TCGTCATCTT CATCGTCCTG CGGACGTCGA 
AGCAACAGCA ACAATGGAAA GTTGCTCGTG GATCCACCTT TGCTAATGAG TCCCGAGATC 
AATTCACTGT TGGGCGACGA AAGGCCTTTG CAGCTGTCGC ACCACCAACA GCAGCAGCAG 
CAAATGCTGC ATCACCATCA ATCGCAGCAA CAGCAGCATC TGCAGCTGAC CCAACAGCAA 
CTGCGTGTAG CTCATCTTGG ACATGGTCTT AGTCACGGTC ACAGCACCAT GCCCACTTTG 
GGCGGACAAC GCAATGGCAA TGGCAATGCC GCCGACGATG GTAAGCTATA CGTAGACCAA 
GCAAAAAACT CGACCAGGGT ATCTGTACCC AAATATGCAC CACAACAAGC ACCACCACCT 
GCACACGTAG CATTCCCGCT AGAGCCACAT ATTCTTCACC AGCACAAATA CCAACGACTA 
GCCACCCAAA CGGTGGCAGA AGATCTTACC ATGAAGCGAA AAGAGCAGGA ATCGGATTCA 
GAACTCGAGC CGGAAGTGGT TGCTGCGTTT GTCGAAGATG GAGGTTCCCA TAGCGGATCC 
TCATCGTCGA GTTCCAGCTC GCACTCAGGA TCTGATTCCA GTTCTGACGT GCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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gpp cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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