Osi2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Osi2
Entrez Gene ID	40756
Full Name	Osiris 2
Synonyms	CG1148,Dmel\CG1148
General protein information	Preferred Names Osiris 2 Names CG1148 gene product from transcript CG1148-RC CG1148-PA CG1148-PB CG1148-PC Osi2-PA Osi2-PB Osi2-PC osiris osiris 2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 83E1-83E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275374.1	NP_001262303.1	osiris 2, isoform C
NM_169130.2	NP_731052.2	osiris 2, isoform A
NM_141364.2	NP_649621.1	osiris 2, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Osi2	NP_649621.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004125	XP_313002.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Osi2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Osi2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa04044	NM_141364.2 Latest version!	Drosophila melanogaster osiris 2, transcript variant B (Osi2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa04044	NM_001275374.1 Latest version!	Drosophila melanogaster osiris 2, transcript variant C (Osi2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa04044	NM_169130.2 Latest version!	Drosophila melanogaster osiris 2, transcript variant A (Osi2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Osi2 ( NM_001275374.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262303.1 Drosophila melanogaster osiris 2, transcript variant C (Osi2), mRNA.

Price & Availability↑

CloneID	OFa04044
Clone ID Related Accession (Same CDS sequence)	NM_001275374.1 , NM_169130.2 , NM_141364.2
Accession Version	NM_001275374.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	osiris 2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAATGA GAGCGCTCAT CTTTCTGGCC CTCGCCACTC TGGTGGCTGG CGAAGGTCTT 
CGGCTTCCGG ACCAGCAGTC GTCCAACAAC ATCCAGCAAG TCTACGCACC GCAGCCACCG 
GCTCAGCAGA TCCAGCAACC CCAGCAGCAA CAACAGTTTC CACAGGCACA GCAGCCAGGA 
GTTGGCGAGG AACGCGGACG CTCCTCCCTG CTCAGCATCT TTGGCCTGGG CAACGACAAC 
GATCCCTTCC TGGCCAGGAC CAACAGCAAC TGCCTCGGCG GCGACCTCTC CGAGTGCTTC 
AAGACTCAGG CGCTCAACAC CTTCGACGAG ATCTTCTTCA AGGACCAGTA CAAGCTCTCC 
GACTTTGCTC GTGTGGTGCG ACTGCCAGAA ACCCAGCAGA GGTCCTTGCT GCAGGAGCCA 
TTCGAGTACT CCGAGGAGCC ACGTGGCGAC GACGACGAGT GGAATCAGCT GCTGAAATAT 
GGACTCCGAA GGGCCGAACG CTTCATCAAG TCCACGGCTC TAGAGGTCGA GTGGCCCGAG 
GAACTCACCG AAGCCGGTCG TTATGAAGCA CGCTTCATCG GAAACGACAT TGATGGCGAA 
CTGGACCTCA TTGATGATGG ACAGCGGGCT GGACATTTCT CGCGTAAGAA GTTGAAGAAG 
ATGATCATTC CCCTGCTGCT GGTGCTGAAG ATCTTCAAGC TGAAGTTGCT GCTCTTCCTG 
CCCTTCATCC TTGGAATCGC TGGCCTCAAG AAGATCCTTG GATTGGCCGC TATTGTCCTG 
CCCGGTCTGT TCGCCTACTT CAAGCTGTGC CGTCCGCCAG GTGGTGTTGG AGGAGCATTC 
GGCGGTGGAC TGTCGGGCCT GTTTGGCGGC AAGAACACCT TCCCCGAGTA CAACCCCCAG 
GGAGTGGGAG CTGCCACCTA CTACCACCAC CACGAGCACT TTGAGGGCGG CCATGGAGGC 
GCTCCAGGAC CTTACTACCG CCAGGAGCCA AGCTTTGCCA AGCCCTACAC CGACTACTAC 
AGCAAGAGCT ACCAGGGCCA GCAGGTCCAG GGCCAGCAGG TTGGCGGCAA CTCCGTCAGC 
TTCGGGGATC CGCAGGAGGC GGCCTACAAC GGATACTATG GACGAAACTC CGGCAAGGAC 
ATTGTCGCCG AGCAACAGCC TCAGAAGAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262303.1
CDS	107..1279
Translation	MAMRALIFLALATLVAGEGLRLPDQQSSNNIQQVYAPQPPAQQIQQPQQQQQFPQAQQPGVGEERGRSSLLSIFGLGNDNDPFLARTNSNCLGGDLSECFKTQALNTFDEIFFKDQYKLSDFARVVRLPETQQRSLLQEPFEYSEEPRGDDDEWNQLLKYGLRRAERFIKSTALEVEWPEELTEAGRYEARFIGNDIDGELDLIDDGQRAGHFSRKKLKKMIIPLLLVLKIFKLKLLLFLPFILGIAGLKKILGLAAIVLPGLFAYFKLCRPPGGVGGAFGGGLSGLFGGKNTFPEYNPQGVGAATYYHHHEHFEGGHGGAPGPYYRQEPSFAKPYTDYYSKSYQGQQVQGQQVGGNSVSFGDPQEAAYNGYYGRNSGKDIVAEQQPQKS

Target ORF information:

RefSeq Version	NM_001275374.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster osiris 2, transcript variant C (Osi2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275374.1

ORF Insert Sequence:

ATGGCAATGA GAGCGCTCAT CTTTCTGGCC CTCGCCACTC TGGTGGCTGG CGAAGGTCTT 
CGGCTTCCGG ACCAGCAGTC GTCCAACAAC ATCCAGCAAG TCTACGCACC GCAGCCACCG 
GCTCAGCAGA TCCAGCAACC CCAGCAGCAA CAACAGTTTC CACAGGCACA GCAGCCAGGA 
GTTGGCGAGG AACGCGGACG CTCCTCCCTG CTCAGCATCT TTGGCCTGGG CAACGACAAC 
GATCCCTTCC TGGCCAGGAC CAACAGCAAC TGCCTCGGCG GCGACCTCTC CGAGTGCTTC 
AAGACTCAGG CGCTCAACAC CTTCGACGAG ATCTTCTTCA AGGACCAGTA CAAGCTCTCC 
GACTTTGCTC GTGTGGTGCG ACTGCCAGAA ACCCAGCAGA GGTCCTTGCT GCAGGAGCCA 
TTCGAGTACT CCGAGGAGCC ACGTGGCGAC GACGACGAGT GGAATCAGCT GCTGAAATAT 
GGACTCCGAA GGGCCGAACG CTTCATCAAG TCCACGGCTC TAGAGGTCGA GTGGCCCGAG 
GAACTCACCG AAGCCGGTCG TTATGAAGCA CGCTTCATCG GAAACGACAT TGATGGCGAA 
CTGGACCTCA TTGATGATGG ACAGCGGGCT GGACATTTCT CGCGTAAGAA GTTGAAGAAG 
ATGATCATTC CCCTGCTGCT GGTGCTGAAG ATCTTCAAGC TGAAGTTGCT GCTCTTCCTG 
CCCTTCATCC TTGGAATCGC TGGCCTCAAG AAGATCCTTG GATTGGCCGC TATTGTCCTG 
CCCGGTCTGT TCGCCTACTT CAAGCTGTGC CGTCCGCCAG GTGGTGTTGG AGGAGCATTC 
GGCGGTGGAC TGTCGGGCCT GTTTGGCGGC AAGAACACCT TCCCCGAGTA CAACCCCCAG 
GGAGTGGGAG CTGCCACCTA CTACCACCAC CACGAGCACT TTGAGGGCGG CCATGGAGGC 
GCTCCAGGAC CTTACTACCG CCAGGAGCCA AGCTTTGCCA AGCCCTACAC CGACTACTAC 
AGCAAGAGCT ACCAGGGCCA GCAGGTCCAG GGCCAGCAGG TTGGCGGCAA CTCCGTCAGC 
TTCGGGGATC CGCAGGAGGC GGCCTACAAC GGATACTATG GACGAAACTC CGGCAAGGAC 
ATTGTCGCCG AGCAACAGCC TCAGAAGAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Osi2 ( NM_169130.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_731052.2 Drosophila melanogaster osiris 2, transcript variant A (Osi2), mRNA.

Price & Availability↑

CloneID	OFa04044
Clone ID Related Accession (Same CDS sequence)	NM_001275374.1 , NM_169130.2 , NM_141364.2
Accession Version	NM_169130.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	osiris 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169130.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAATGA GAGCGCTCAT CTTTCTGGCC CTCGCCACTC TGGTGGCTGG CGAAGGTCTT 
CGGCTTCCGG ACCAGCAGTC GTCCAACAAC ATCCAGCAAG TCTACGCACC GCAGCCACCG 
GCTCAGCAGA TCCAGCAACC CCAGCAGCAA CAACAGTTTC CACAGGCACA GCAGCCAGGA 
GTTGGCGAGG AACGCGGACG CTCCTCCCTG CTCAGCATCT TTGGCCTGGG CAACGACAAC 
GATCCCTTCC TGGCCAGGAC CAACAGCAAC TGCCTCGGCG GCGACCTCTC CGAGTGCTTC 
AAGACTCAGG CGCTCAACAC CTTCGACGAG ATCTTCTTCA AGGACCAGTA CAAGCTCTCC 
GACTTTGCTC GTGTGGTGCG ACTGCCAGAA ACCCAGCAGA GGTCCTTGCT GCAGGAGCCA 
TTCGAGTACT CCGAGGAGCC ACGTGGCGAC GACGACGAGT GGAATCAGCT GCTGAAATAT 
GGACTCCGAA GGGCCGAACG CTTCATCAAG TCCACGGCTC TAGAGGTCGA GTGGCCCGAG 
GAACTCACCG AAGCCGGTCG TTATGAAGCA CGCTTCATCG GAAACGACAT TGATGGCGAA 
CTGGACCTCA TTGATGATGG ACAGCGGGCT GGACATTTCT CGCGTAAGAA GTTGAAGAAG 
ATGATCATTC CCCTGCTGCT GGTGCTGAAG ATCTTCAAGC TGAAGTTGCT GCTCTTCCTG 
CCCTTCATCC TTGGAATCGC TGGCCTCAAG AAGATCCTTG GATTGGCCGC TATTGTCCTG 
CCCGGTCTGT TCGCCTACTT CAAGCTGTGC CGTCCGCCAG GTGGTGTTGG AGGAGCATTC 
GGCGGTGGAC TGTCGGGCCT GTTTGGCGGC AAGAACACCT TCCCCGAGTA CAACCCCCAG 
GGAGTGGGAG CTGCCACCTA CTACCACCAC CACGAGCACT TTGAGGGCGG CCATGGAGGC 
GCTCCAGGAC CTTACTACCG CCAGGAGCCA AGCTTTGCCA AGCCCTACAC CGACTACTAC 
AGCAAGAGCT ACCAGGGCCA GCAGGTCCAG GGCCAGCAGG TTGGCGGCAA CTCCGTCAGC 
TTCGGGGATC CGCAGGAGGC GGCCTACAAC GGATACTATG GACGAAACTC CGGCAAGGAC 
ATTGTCGCCG AGCAACAGCC TCAGAAGAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_731052.2
CDS	190..1362
Translation	MAMRALIFLALATLVAGEGLRLPDQQSSNNIQQVYAPQPPAQQIQQPQQQQQFPQAQQPGVGEERGRSSLLSIFGLGNDNDPFLARTNSNCLGGDLSECFKTQALNTFDEIFFKDQYKLSDFARVVRLPETQQRSLLQEPFEYSEEPRGDDDEWNQLLKYGLRRAERFIKSTALEVEWPEELTEAGRYEARFIGNDIDGELDLIDDGQRAGHFSRKKLKKMIIPLLLVLKIFKLKLLLFLPFILGIAGLKKILGLAAIVLPGLFAYFKLCRPPGGVGGAFGGGLSGLFGGKNTFPEYNPQGVGAATYYHHHEHFEGGHGGAPGPYYRQEPSFAKPYTDYYSKSYQGQQVQGQQVGGNSVSFGDPQEAAYNGYYGRNSGKDIVAEQQPQKS

Target ORF information:

RefSeq Version	NM_169130.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster osiris 2, transcript variant A (Osi2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_169130.2

ORF Insert Sequence:

ATGGCAATGA GAGCGCTCAT CTTTCTGGCC CTCGCCACTC TGGTGGCTGG CGAAGGTCTT 
CGGCTTCCGG ACCAGCAGTC GTCCAACAAC ATCCAGCAAG TCTACGCACC GCAGCCACCG 
GCTCAGCAGA TCCAGCAACC CCAGCAGCAA CAACAGTTTC CACAGGCACA GCAGCCAGGA 
GTTGGCGAGG AACGCGGACG CTCCTCCCTG CTCAGCATCT TTGGCCTGGG CAACGACAAC 
GATCCCTTCC TGGCCAGGAC CAACAGCAAC TGCCTCGGCG GCGACCTCTC CGAGTGCTTC 
AAGACTCAGG CGCTCAACAC CTTCGACGAG ATCTTCTTCA AGGACCAGTA CAAGCTCTCC 
GACTTTGCTC GTGTGGTGCG ACTGCCAGAA ACCCAGCAGA GGTCCTTGCT GCAGGAGCCA 
TTCGAGTACT CCGAGGAGCC ACGTGGCGAC GACGACGAGT GGAATCAGCT GCTGAAATAT 
GGACTCCGAA GGGCCGAACG CTTCATCAAG TCCACGGCTC TAGAGGTCGA GTGGCCCGAG 
GAACTCACCG AAGCCGGTCG TTATGAAGCA CGCTTCATCG GAAACGACAT TGATGGCGAA 
CTGGACCTCA TTGATGATGG ACAGCGGGCT GGACATTTCT CGCGTAAGAA GTTGAAGAAG 
ATGATCATTC CCCTGCTGCT GGTGCTGAAG ATCTTCAAGC TGAAGTTGCT GCTCTTCCTG 
CCCTTCATCC TTGGAATCGC TGGCCTCAAG AAGATCCTTG GATTGGCCGC TATTGTCCTG 
CCCGGTCTGT TCGCCTACTT CAAGCTGTGC CGTCCGCCAG GTGGTGTTGG AGGAGCATTC 
GGCGGTGGAC TGTCGGGCCT GTTTGGCGGC AAGAACACCT TCCCCGAGTA CAACCCCCAG 
GGAGTGGGAG CTGCCACCTA CTACCACCAC CACGAGCACT TTGAGGGCGG CCATGGAGGC 
GCTCCAGGAC CTTACTACCG CCAGGAGCCA AGCTTTGCCA AGCCCTACAC CGACTACTAC 
AGCAAGAGCT ACCAGGGCCA GCAGGTCCAG GGCCAGCAGG TTGGCGGCAA CTCCGTCAGC 
TTCGGGGATC CGCAGGAGGC GGCCTACAAC GGATACTATG GACGAAACTC CGGCAAGGAC 
ATTGTCGCCG AGCAACAGCC TCAGAAGAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Osi2 ( NM_141364.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649621.1 Drosophila melanogaster osiris 2, transcript variant B (Osi2), mRNA.

Price & Availability↑

CloneID	OFa04044
Clone ID Related Accession (Same CDS sequence)	NM_001275374.1 , NM_169130.2 , NM_141364.2
Accession Version	NM_141364.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	osiris 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141364.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCAATGA GAGCGCTCAT CTTTCTGGCC CTCGCCACTC TGGTGGCTGG CGAAGGTCTT 
CGGCTTCCGG ACCAGCAGTC GTCCAACAAC ATCCAGCAAG TCTACGCACC GCAGCCACCG 
GCTCAGCAGA TCCAGCAACC CCAGCAGCAA CAACAGTTTC CACAGGCACA GCAGCCAGGA 
GTTGGCGAGG AACGCGGACG CTCCTCCCTG CTCAGCATCT TTGGCCTGGG CAACGACAAC 
GATCCCTTCC TGGCCAGGAC CAACAGCAAC TGCCTCGGCG GCGACCTCTC CGAGTGCTTC 
AAGACTCAGG CGCTCAACAC CTTCGACGAG ATCTTCTTCA AGGACCAGTA CAAGCTCTCC 
GACTTTGCTC GTGTGGTGCG ACTGCCAGAA ACCCAGCAGA GGTCCTTGCT GCAGGAGCCA 
TTCGAGTACT CCGAGGAGCC ACGTGGCGAC GACGACGAGT GGAATCAGCT GCTGAAATAT 
GGACTCCGAA GGGCCGAACG CTTCATCAAG TCCACGGCTC TAGAGGTCGA GTGGCCCGAG 
GAACTCACCG AAGCCGGTCG TTATGAAGCA CGCTTCATCG GAAACGACAT TGATGGCGAA 
CTGGACCTCA TTGATGATGG ACAGCGGGCT GGACATTTCT CGCGTAAGAA GTTGAAGAAG 
ATGATCATTC CCCTGCTGCT GGTGCTGAAG ATCTTCAAGC TGAAGTTGCT GCTCTTCCTG 
CCCTTCATCC TTGGAATCGC TGGCCTCAAG AAGATCCTTG GATTGGCCGC TATTGTCCTG 
CCCGGTCTGT TCGCCTACTT CAAGCTGTGC CGTCCGCCAG GTGGTGTTGG AGGAGCATTC 
GGCGGTGGAC TGTCGGGCCT GTTTGGCGGC AAGAACACCT TCCCCGAGTA CAACCCCCAG 
GGAGTGGGAG CTGCCACCTA CTACCACCAC CACGAGCACT TTGAGGGCGG CCATGGAGGC 
GCTCCAGGAC CTTACTACCG CCAGGAGCCA AGCTTTGCCA AGCCCTACAC CGACTACTAC 
AGCAAGAGCT ACCAGGGCCA GCAGGTCCAG GGCCAGCAGG TTGGCGGCAA CTCCGTCAGC 
TTCGGGGATC CGCAGGAGGC GGCCTACAAC GGATACTATG GACGAAACTC CGGCAAGGAC 
ATTGTCGCCG AGCAACAGCC TCAGAAGAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649621.1
CDS	165..1337
Translation	MAMRALIFLALATLVAGEGLRLPDQQSSNNIQQVYAPQPPAQQIQQPQQQQQFPQAQQPGVGEERGRSSLLSIFGLGNDNDPFLARTNSNCLGGDLSECFKTQALNTFDEIFFKDQYKLSDFARVVRLPETQQRSLLQEPFEYSEEPRGDDDEWNQLLKYGLRRAERFIKSTALEVEWPEELTEAGRYEARFIGNDIDGELDLIDDGQRAGHFSRKKLKKMIIPLLLVLKIFKLKLLLFLPFILGIAGLKKILGLAAIVLPGLFAYFKLCRPPGGVGGAFGGGLSGLFGGKNTFPEYNPQGVGAATYYHHHEHFEGGHGGAPGPYYRQEPSFAKPYTDYYSKSYQGQQVQGQQVGGNSVSFGDPQEAAYNGYYGRNSGKDIVAEQQPQKS

Target ORF information:

RefSeq Version	NM_141364.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster osiris 2, transcript variant B (Osi2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141364.2

ORF Insert Sequence:

ATGGCAATGA GAGCGCTCAT CTTTCTGGCC CTCGCCACTC TGGTGGCTGG CGAAGGTCTT 
CGGCTTCCGG ACCAGCAGTC GTCCAACAAC ATCCAGCAAG TCTACGCACC GCAGCCACCG 
GCTCAGCAGA TCCAGCAACC CCAGCAGCAA CAACAGTTTC CACAGGCACA GCAGCCAGGA 
GTTGGCGAGG AACGCGGACG CTCCTCCCTG CTCAGCATCT TTGGCCTGGG CAACGACAAC 
GATCCCTTCC TGGCCAGGAC CAACAGCAAC TGCCTCGGCG GCGACCTCTC CGAGTGCTTC 
AAGACTCAGG CGCTCAACAC CTTCGACGAG ATCTTCTTCA AGGACCAGTA CAAGCTCTCC 
GACTTTGCTC GTGTGGTGCG ACTGCCAGAA ACCCAGCAGA GGTCCTTGCT GCAGGAGCCA 
TTCGAGTACT CCGAGGAGCC ACGTGGCGAC GACGACGAGT GGAATCAGCT GCTGAAATAT 
GGACTCCGAA GGGCCGAACG CTTCATCAAG TCCACGGCTC TAGAGGTCGA GTGGCCCGAG 
GAACTCACCG AAGCCGGTCG TTATGAAGCA CGCTTCATCG GAAACGACAT TGATGGCGAA 
CTGGACCTCA TTGATGATGG ACAGCGGGCT GGACATTTCT CGCGTAAGAA GTTGAAGAAG 
ATGATCATTC CCCTGCTGCT GGTGCTGAAG ATCTTCAAGC TGAAGTTGCT GCTCTTCCTG 
CCCTTCATCC TTGGAATCGC TGGCCTCAAG AAGATCCTTG GATTGGCCGC TATTGTCCTG 
CCCGGTCTGT TCGCCTACTT CAAGCTGTGC CGTCCGCCAG GTGGTGTTGG AGGAGCATTC 
GGCGGTGGAC TGTCGGGCCT GTTTGGCGGC AAGAACACCT TCCCCGAGTA CAACCCCCAG 
GGAGTGGGAG CTGCCACCTA CTACCACCAC CACGAGCACT TTGAGGGCGG CCATGGAGGC 
GCTCCAGGAC CTTACTACCG CCAGGAGCCA AGCTTTGCCA AGCCCTACAC CGACTACTAC 
AGCAAGAGCT ACCAGGGCCA GCAGGTCCAG GGCCAGCAGG TTGGCGGCAA CTCCGTCAGC 
TTCGGGGATC CGCAGGAGGC GGCCTACAAC GGATACTATG GACGAAACTC CGGCAAGGAC 
ATTGTCGCCG AGCAACAGCC TCAGAAGAGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Osi2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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