mia cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mia
Entrez Gene ID	40667
Full Name	meiosis I arrest
Synonyms	CG10390,Dmel\CG10390,Mia,TAF,TAF60-2,TAF6L,TAF[[II]],TAF[[II]]60-2,TFIID,Taf60-2,Taf6L,dTAF6L,dTAF[[II]]60,dTAF[[II]]60-2,dmTAF6b,ms(3)570
General protein information	Preferred Names meiosis I arrest Names CG10390 gene product from transcript CG10390-RA CG10390-PA CG10390-PB CG10390-PC TAF6-like TBP-associated factor 6-like TBP-associated factor 60kD-2 mia-PA mia-PB mia-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 83B1-83B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_141290.6	NP_649547.3	meiosis I arrest, isoform A
NM_001144538.2	NP_001138010.1	meiosis I arrest, isoform B
NM_001300252.1	NP_001287181.1	meiosis I arrest, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mia gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mia cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa03946	NM_141290.6 Latest version!	Drosophila melanogaster meiosis I arrest (mia), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa03946	NM_001144538.2 Latest version!	Drosophila melanogaster meiosis I arrest (mia), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa03946	NM_001300252.1 Latest version!	Drosophila melanogaster meiosis I arrest (mia), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mia ( NM_141290.6 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649547.3 Drosophila melanogaster meiosis I arrest (mia), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa03946
Clone ID Related Accession (Same CDS sequence)	NM_141290.6 , NM_001144538.2 , NM_001300252.1
Accession Version	NM_141290.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	meiosis I arrest, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_141290.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGCAGT TTGAAAACAA AAACGCAACT AAAATGAAAA CGTCTCAGAA TGAAATGCCC 
GACAAGGACA AAAGTTTTCG CAGACATTCT TTGTCGCCGC GTTCTCTATC CGCGATATTC 
TTCCACAGCA CTGGTCTGCA TCTGGACGAC GGAGTAGCAC AGTGGCTATC CATCAATGTT 
AAGGAAGACA TTACAAATCT GCTAAACGAG GCTGGAAAAT ACATGCGGCG CATCCGGGAT 
CGGCGATTGC AACTGTCTCA CATCCAGCAT GCCGTCCGAA TGCACGACGA CCTCTGCTAT 
GACATCTTTT TTAGGCTGGT CCATTGCGAC GATTGCAAAA TGCCTCCTTC CCAAAAAGTA 
TTAAAAACCG TCCGCGAAGC TGTAACCGCC GAAAAAAAGG ATGAACTTCT CGTTTCCTAC 
CCGGAATCCG TGCAGGAATC TGTGCCGGAA CCCGTGCCGG AGACTTCATT GGAGCCGCCT 
CCCATGCACA CCGGGTGGCT AAAGGTGGAG CAGGTGCTGC TAAAGCCCAG CAAGCGCTAT 
CCGCTGAGCA TGGAACAGCA GAACTTCTTC GAGTTGGTAA CCGAGGCCTG CGTAGGCGAC 
TTGGAATCCC GGCGCGTGCT TGCTCTCAAA GCCATCTCCA CTGATCCCTC GCTGGAGGAG 
TTGCTGCCCA GGTTGACCAA GTTCATTGCG GACGCAGTGG CTATCAACGT GGCCCAGCAG 
AACCTGCCGC TGCTCCTTTA CCTTATGCGC ATGGTCCGAG CTCTTTTGGG AAACCAGCGC 
TTCAGCCTAT TGCAATATCT CCACCTGCTC CTGCCAGCCG TTCTATCCTG CCTGCTGGCC 
AAACAAGTGT GCGCATCTCC GAACTCGGAG GATCACTGGG CCTTAAGGGA ATACTCCGGA 
AACATAATGG CCCACATAGT GCGCCAGTTC GACGCTGCCG ACAACGGCAT CCTGCCCAGA 
GTCATTGGTG TTTACAACAA GGCGCTATTG AAGAAGCCAC TGACAACGGT GTTTGGGGCC 
GTCATAGGCC TGGGAAAGAT GGGGAATCAT GCGGTCCGCG CATGCATTTT GCCTCAATTG 
AAGTACCTAT CTGAACACAT CGATTCACAC ATGGCCGCCA GTAATGACTC TCCCAGCTCC 
AGCGTAGACA GGCAAGCAGT TAAGTACATT CGTCATCGGT TGATGAAGAT GTGCTCTCCC 
GTGCTGATGG GCATACATCA ACCCCCTGAT CTGCCGGAAG AGTTTATGGA GAGATATGGA 
TCTTTGGGCA CGCTGATGAG CGATGGCGTC ACCGTGATGC GTACGAAGAA ACAAGCAGTG 
GCTGCAGCCA AGGCGGAAGC AGAACTGAAG TTGAAAAATA TGGCTACAGA ACACCTTAAC 
TCCAGAGGGA AAGGATCTCG ATCCGTCGGC TCCAACGATC TTGTCTCTGG CACCCAGCTG 
CCCAAGAATG GTATTCGTCC CACAAGCCAA GCACCCAAAT CTGTCTATTC AATTTCTGTG 
AATGGACAGA CCGTCAAAAT TTCGACGCCA AATCTTTGCA ATACAATGCT TCAGCATCAA 
AAGAAGGATT TATTTTCCAA ACCATCGGAC TTTGTGGTTC CTCAATTAGT GGTTAATCAA 
TTCAAAAACG GCTTAATCCC GTCCAATTCC CAGCGATCCA GTTCGCCGGC CAGGAACGTG 
ATACATTTGA AACCTAACAA CGACCATCCT TCAAAAATGG CTTTGTTCCT ACGTCAAGCA 
ATAACGGCAG ATTGTCCAAT AATTACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649547.3
CDS	13..1782
Translation	MWQFENKNATKMKTSQNEMPDKDKSFRRHSLSPRSLSAIFFHSTGLHLDDGVAQWLSINVKEDITNLLNEAGKYMRRIRDRRLQLSHIQHAVRMHDDLCYDIFFRLVHCDDCKMPPSQKVLKTVREAVTAEKKDELLVSYPESVQESVPEPVPETSLEPPPMHTGWLKVEQVLLKPSKRYPLSMEQQNFFELVTEACVGDLESRRVLALKAISTDPSLEELLPRLTKFIADAVAINVAQQNLPLLLYLMRMVRALLGNQRFSLLQYLHLLLPAVLSCLLAKQVCASPNSEDHWALREYSGNIMAHIVRQFDAADNGILPRVIGVYNKALLKKPLTTVFGAVIGLGKMGNHAVRACILPQLKYLSEHIDSHMAASNDSPSSSVDRQAVKYIRHRLMKMCSPVLMGIHQPPDLPEEFMERYGSLGTLMSDGVTVMRTKKQAVAAAKAEAELKLKNMATEHLNSRGKGSRSVGSNDLVSGTQLPKNGIRPTSQAPKSVYSISVNGQTVKISTPNLCNTMLQHQKKDLFSKPSDFVVPQLVVNQFKNGLIPSNSQRSSSPARNVIHLKPNNDHPSKMALFLRQAITADCPIIT

Target ORF information:

RefSeq Version	NM_141290.6
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster meiosis I arrest (mia), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141290.6

ORF Insert Sequence:

ATGTGGCAGT TTGAAAACAA AAACGCAACT AAAATGAAAA CGTCTCAGAA TGAAATGCCC 
GACAAGGACA AAAGTTTTCG CAGACATTCT TTGTCGCCGC GTTCTCTATC CGCGATATTC 
TTCCACAGCA CTGGTCTGCA TCTGGACGAC GGAGTAGCAC AGTGGCTATC CATCAATGTT 
AAGGAAGACA TTACAAATCT GCTAAACGAG GCTGGAAAAT ACATGCGGCG CATCCGGGAT 
CGGCGATTGC AACTGTCTCA CATCCAGCAT GCCGTCCGAA TGCACGACGA CCTCTGCTAT 
GACATCTTTT TTAGGCTGGT CCATTGCGAC GATTGCAAAA TGCCTCCTTC CCAAAAAGTA 
TTAAAAACCG TCCGCGAAGC TGTAACCGCC GAAAAAAAGG ATGAACTTCT CGTTTCCTAC 
CCGGAATCCG TGCAGGAATC TGTGCCGGAA CCCGTGCCGG AGACTTCATT GGAGCCGCCT 
CCCATGCACA CCGGGTGGCT AAAGGTGGAG CAGGTGCTGC TAAAGCCCAG CAAGCGCTAT 
CCGCTGAGCA TGGAACAGCA GAACTTCTTC GAGTTGGTAA CCGAGGCCTG CGTAGGCGAC 
TTGGAATCCC GGCGCGTGCT TGCTCTCAAA GCCATCTCCA CTGATCCCTC GCTGGAGGAG 
TTGCTGCCCA GGTTGACCAA GTTCATTGCG GACGCAGTGG CTATCAACGT GGCCCAGCAG 
AACCTGCCGC TGCTCCTTTA CCTTATGCGC ATGGTCCGAG CTCTTTTGGG AAACCAGCGC 
TTCAGCCTAT TGCAATATCT CCACCTGCTC CTGCCAGCCG TTCTATCCTG CCTGCTGGCC 
AAACAAGTGT GCGCATCTCC GAACTCGGAG GATCACTGGG CCTTAAGGGA ATACTCCGGA 
AACATAATGG CCCACATAGT GCGCCAGTTC GACGCTGCCG ACAACGGCAT CCTGCCCAGA 
GTCATTGGTG TTTACAACAA GGCGCTATTG AAGAAGCCAC TGACAACGGT GTTTGGGGCC 
GTCATAGGCC TGGGAAAGAT GGGGAATCAT GCGGTCCGCG CATGCATTTT GCCTCAATTG 
AAGTACCTAT CTGAACACAT CGATTCACAC ATGGCCGCCA GTAATGACTC TCCCAGCTCC 
AGCGTAGACA GGCAAGCAGT TAAGTACATT CGTCATCGGT TGATGAAGAT GTGCTCTCCC 
GTGCTGATGG GCATACATCA ACCCCCTGAT CTGCCGGAAG AGTTTATGGA GAGATATGGA 
TCTTTGGGCA CGCTGATGAG CGATGGCGTC ACCGTGATGC GTACGAAGAA ACAAGCAGTG 
GCTGCAGCCA AGGCGGAAGC AGAACTGAAG TTGAAAAATA TGGCTACAGA ACACCTTAAC 
TCCAGAGGGA AAGGATCTCG ATCCGTCGGC TCCAACGATC TTGTCTCTGG CACCCAGCTG 
CCCAAGAATG GTATTCGTCC CACAAGCCAA GCACCCAAAT CTGTCTATTC AATTTCTGTG 
AATGGACAGA CCGTCAAAAT TTCGACGCCA AATCTTTGCA ATACAATGCT TCAGCATCAA 
AAGAAGGATT TATTTTCCAA ACCATCGGAC TTTGTGGTTC CTCAATTAGT GGTTAATCAA 
TTCAAAAACG GCTTAATCCC GTCCAATTCC CAGCGATCCA GTTCGCCGGC CAGGAACGTG 
ATACATTTGA AACCTAACAA CGACCATCCT TCAAAAATGG CTTTGTTCCT ACGTCAAGCA 
ATAACGGCAG ATTGTCCAAT AATTACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mia ( NM_001144538.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001138010.1 Drosophila melanogaster meiosis I arrest (mia), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa03946
Clone ID Related Accession (Same CDS sequence)	NM_141290.6 , NM_001144538.2 , NM_001300252.1
Accession Version	NM_001144538.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	meiosis I arrest, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001144538.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGCAGT TTGAAAACAA AAACGCAACT AAAATGAAAA CGTCTCAGAA TGAAATGCCC 
GACAAGGACA AAAGTTTTCG CAGACATTCT TTGTCGCCGC GTTCTCTATC CGCGATATTC 
TTCCACAGCA CTGGTCTGCA TCTGGACGAC GGAGTAGCAC AGTGGCTATC CATCAATGTT 
AAGGAAGACA TTACAAATCT GCTAAACGAG GCTGGAAAAT ACATGCGGCG CATCCGGGAT 
CGGCGATTGC AACTGTCTCA CATCCAGCAT GCCGTCCGAA TGCACGACGA CCTCTGCTAT 
GACATCTTTT TTAGGCTGGT CCATTGCGAC GATTGCAAAA TGCCTCCTTC CCAAAAAGTA 
TTAAAAACCG TCCGCGAAGC TGTAACCGCC GAAAAAAAGG ATGAACTTCT CGTTTCCTAC 
CCGGAATCCG TGCAGGAATC TGTGCCGGAA CCCGTGCCGG AGACTTCATT GGAGCCGCCT 
CCCATGCACA CCGGGTGGCT AAAGGTGGAG CAGGTGCTGC TAAAGCCCAG CAAGCGCTAT 
CCGCTGAGCA TGGAACAGCA GAACTTCTTC GAGTTGGTAA CCGAGGCCTG CGTAGGCGAC 
TTGGAATCCC GGCGCGTGCT TGCTCTCAAA GCCATCTCCA CTGATCCCTC GCTGGAGGAG 
TTGCTGCCCA GGTTGACCAA GTTCATTGCG GACGCAGTGG CTATCAACGT GGCCCAGCAG 
AACCTGCCGC TGCTCCTTTA CCTTATGCGC ATGGTCCGAG CTCTTTTGGG AAACCAGCGC 
TTCAGCCTAT TGCAATATCT CCACCTGCTC CTGCCAGCCG TTCTATCCTG CCTGCTGGCC 
AAACAAGTGT GCGCATCTCC GAACTCGGAG GATCACTGGG CCTTAAGGGA ATACTCCGGA 
AACATAATGG CCCACATAGT GCGCCAGTTC GACGCTGCCG ACAACGGCAT CCTGCCCAGA 
GTCATTGGTG TTTACAACAA GGCGCTATTG AAGAAGCCAC TGACAACGGT GTTTGGGGCC 
GTCATAGGCC TGGGAAAGAT GGGGAATCAT GCGGTCCGCG CATGCATTTT GCCTCAATTG 
AAGTACCTAT CTGAACACAT CGATTCACAC ATGGCCGCCA GTAATGACTC TCCCAGCTCC 
AGCGTAGACA GGCAAGCAGT TAAGTACATT CGTCATCGGT TGATGAAGAT GTGCTCTCCC 
GTGCTGATGG GCATACATCA ACCCCCTGAT CTGCCGGAAG AGTTTATGGA GAGATATGGA 
TCTTTGGGCA CGCTGATGAG CGATGGCGTC ACCGTGATGC GTACGAAGAA ACAAGCAGTG 
GCTGCAGCCA AGGCGGAAGC AGAACTGAAG TTGAAAAATA TGGCTACAGA ACACCTTAAC 
TCCAGAGGGA AAGGATCTCG ATCCGTCGGC TCCAACGATC TTGTCTCTGG CACCCAGCTG 
CCCAAGAATG GTATTCGTCC CACAAGCCAA GCACCCAAAT CTGTCTATTC AATTTCTGTG 
AATGGACAGA CCGTCAAAAT TTCGACGCCA AATCTTTGCA ATACAATGCT TCAGCATCAA 
AAGAAGGATT TATTTTCCAA ACCATCGGAC TTTGTGGTTC CTCAATTAGT GGTTAATCAA 
TTCAAAAACG GCTTAATCCC GTCCAATTCC CAGCGATCCA GTTCGCCGGC CAGGAACGTG 
ATACATTTGA AACCTAACAA CGACCATCCT TCAAAAATGG CTTTGTTCCT ACGTCAAGCA 
ATAACGGCAG ATTGTCCAAT AATTACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138010.1
CDS	13..1782
Translation	MWQFENKNATKMKTSQNEMPDKDKSFRRHSLSPRSLSAIFFHSTGLHLDDGVAQWLSINVKEDITNLLNEAGKYMRRIRDRRLQLSHIQHAVRMHDDLCYDIFFRLVHCDDCKMPPSQKVLKTVREAVTAEKKDELLVSYPESVQESVPEPVPETSLEPPPMHTGWLKVEQVLLKPSKRYPLSMEQQNFFELVTEACVGDLESRRVLALKAISTDPSLEELLPRLTKFIADAVAINVAQQNLPLLLYLMRMVRALLGNQRFSLLQYLHLLLPAVLSCLLAKQVCASPNSEDHWALREYSGNIMAHIVRQFDAADNGILPRVIGVYNKALLKKPLTTVFGAVIGLGKMGNHAVRACILPQLKYLSEHIDSHMAASNDSPSSSVDRQAVKYIRHRLMKMCSPVLMGIHQPPDLPEEFMERYGSLGTLMSDGVTVMRTKKQAVAAAKAEAELKLKNMATEHLNSRGKGSRSVGSNDLVSGTQLPKNGIRPTSQAPKSVYSISVNGQTVKISTPNLCNTMLQHQKKDLFSKPSDFVVPQLVVNQFKNGLIPSNSQRSSSPARNVIHLKPNNDHPSKMALFLRQAITADCPIIT

Target ORF information:

RefSeq Version	NM_001144538.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster meiosis I arrest (mia), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144538.2

ORF Insert Sequence:

ATGTGGCAGT TTGAAAACAA AAACGCAACT AAAATGAAAA CGTCTCAGAA TGAAATGCCC 
GACAAGGACA AAAGTTTTCG CAGACATTCT TTGTCGCCGC GTTCTCTATC CGCGATATTC 
TTCCACAGCA CTGGTCTGCA TCTGGACGAC GGAGTAGCAC AGTGGCTATC CATCAATGTT 
AAGGAAGACA TTACAAATCT GCTAAACGAG GCTGGAAAAT ACATGCGGCG CATCCGGGAT 
CGGCGATTGC AACTGTCTCA CATCCAGCAT GCCGTCCGAA TGCACGACGA CCTCTGCTAT 
GACATCTTTT TTAGGCTGGT CCATTGCGAC GATTGCAAAA TGCCTCCTTC CCAAAAAGTA 
TTAAAAACCG TCCGCGAAGC TGTAACCGCC GAAAAAAAGG ATGAACTTCT CGTTTCCTAC 
CCGGAATCCG TGCAGGAATC TGTGCCGGAA CCCGTGCCGG AGACTTCATT GGAGCCGCCT 
CCCATGCACA CCGGGTGGCT AAAGGTGGAG CAGGTGCTGC TAAAGCCCAG CAAGCGCTAT 
CCGCTGAGCA TGGAACAGCA GAACTTCTTC GAGTTGGTAA CCGAGGCCTG CGTAGGCGAC 
TTGGAATCCC GGCGCGTGCT TGCTCTCAAA GCCATCTCCA CTGATCCCTC GCTGGAGGAG 
TTGCTGCCCA GGTTGACCAA GTTCATTGCG GACGCAGTGG CTATCAACGT GGCCCAGCAG 
AACCTGCCGC TGCTCCTTTA CCTTATGCGC ATGGTCCGAG CTCTTTTGGG AAACCAGCGC 
TTCAGCCTAT TGCAATATCT CCACCTGCTC CTGCCAGCCG TTCTATCCTG CCTGCTGGCC 
AAACAAGTGT GCGCATCTCC GAACTCGGAG GATCACTGGG CCTTAAGGGA ATACTCCGGA 
AACATAATGG CCCACATAGT GCGCCAGTTC GACGCTGCCG ACAACGGCAT CCTGCCCAGA 
GTCATTGGTG TTTACAACAA GGCGCTATTG AAGAAGCCAC TGACAACGGT GTTTGGGGCC 
GTCATAGGCC TGGGAAAGAT GGGGAATCAT GCGGTCCGCG CATGCATTTT GCCTCAATTG 
AAGTACCTAT CTGAACACAT CGATTCACAC ATGGCCGCCA GTAATGACTC TCCCAGCTCC 
AGCGTAGACA GGCAAGCAGT TAAGTACATT CGTCATCGGT TGATGAAGAT GTGCTCTCCC 
GTGCTGATGG GCATACATCA ACCCCCTGAT CTGCCGGAAG AGTTTATGGA GAGATATGGA 
TCTTTGGGCA CGCTGATGAG CGATGGCGTC ACCGTGATGC GTACGAAGAA ACAAGCAGTG 
GCTGCAGCCA AGGCGGAAGC AGAACTGAAG TTGAAAAATA TGGCTACAGA ACACCTTAAC 
TCCAGAGGGA AAGGATCTCG ATCCGTCGGC TCCAACGATC TTGTCTCTGG CACCCAGCTG 
CCCAAGAATG GTATTCGTCC CACAAGCCAA GCACCCAAAT CTGTCTATTC AATTTCTGTG 
AATGGACAGA CCGTCAAAAT TTCGACGCCA AATCTTTGCA ATACAATGCT TCAGCATCAA 
AAGAAGGATT TATTTTCCAA ACCATCGGAC TTTGTGGTTC CTCAATTAGT GGTTAATCAA 
TTCAAAAACG GCTTAATCCC GTCCAATTCC CAGCGATCCA GTTCGCCGGC CAGGAACGTG 
ATACATTTGA AACCTAACAA CGACCATCCT TCAAAAATGG CTTTGTTCCT ACGTCAAGCA 
ATAACGGCAG ATTGTCCAAT AATTACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mia ( NM_001300252.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287181.1 Drosophila melanogaster meiosis I arrest (mia), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa03946
Clone ID Related Accession (Same CDS sequence)	NM_141290.6 , NM_001144538.2 , NM_001300252.1
Accession Version	NM_001300252.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	meiosis I arrest, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGGCAGT TTGAAAACAA AAACGCAACT AAAATGAAAA CGTCTCAGAA TGAAATGCCC 
GACAAGGACA AAAGTTTTCG CAGACATTCT TTGTCGCCGC GTTCTCTATC CGCGATATTC 
TTCCACAGCA CTGGTCTGCA TCTGGACGAC GGAGTAGCAC AGTGGCTATC CATCAATGTT 
AAGGAAGACA TTACAAATCT GCTAAACGAG GCTGGAAAAT ACATGCGGCG CATCCGGGAT 
CGGCGATTGC AACTGTCTCA CATCCAGCAT GCCGTCCGAA TGCACGACGA CCTCTGCTAT 
GACATCTTTT TTAGGCTGGT CCATTGCGAC GATTGCAAAA TGCCTCCTTC CCAAAAAGTA 
TTAAAAACCG TCCGCGAAGC TGTAACCGCC GAAAAAAAGG ATGAACTTCT CGTTTCCTAC 
CCGGAATCCG TGCAGGAATC TGTGCCGGAA CCCGTGCCGG AGACTTCATT GGAGCCGCCT 
CCCATGCACA CCGGGTGGCT AAAGGTGGAG CAGGTGCTGC TAAAGCCCAG CAAGCGCTAT 
CCGCTGAGCA TGGAACAGCA GAACTTCTTC GAGTTGGTAA CCGAGGCCTG CGTAGGCGAC 
TTGGAATCCC GGCGCGTGCT TGCTCTCAAA GCCATCTCCA CTGATCCCTC GCTGGAGGAG 
TTGCTGCCCA GGTTGACCAA GTTCATTGCG GACGCAGTGG CTATCAACGT GGCCCAGCAG 
AACCTGCCGC TGCTCCTTTA CCTTATGCGC ATGGTCCGAG CTCTTTTGGG AAACCAGCGC 
TTCAGCCTAT TGCAATATCT CCACCTGCTC CTGCCAGCCG TTCTATCCTG CCTGCTGGCC 
AAACAAGTGT GCGCATCTCC GAACTCGGAG GATCACTGGG CCTTAAGGGA ATACTCCGGA 
AACATAATGG CCCACATAGT GCGCCAGTTC GACGCTGCCG ACAACGGCAT CCTGCCCAGA 
GTCATTGGTG TTTACAACAA GGCGCTATTG AAGAAGCCAC TGACAACGGT GTTTGGGGCC 
GTCATAGGCC TGGGAAAGAT GGGGAATCAT GCGGTCCGCG CATGCATTTT GCCTCAATTG 
AAGTACCTAT CTGAACACAT CGATTCACAC ATGGCCGCCA GTAATGACTC TCCCAGCTCC 
AGCGTAGACA GGCAAGCAGT TAAGTACATT CGTCATCGGT TGATGAAGAT GTGCTCTCCC 
GTGCTGATGG GCATACATCA ACCCCCTGAT CTGCCGGAAG AGTTTATGGA GAGATATGGA 
TCTTTGGGCA CGCTGATGAG CGATGGCGTC ACCGTGATGC GTACGAAGAA ACAAGCAGTG 
GCTGCAGCCA AGGCGGAAGC AGAACTGAAG TTGAAAAATA TGGCTACAGA ACACCTTAAC 
TCCAGAGGGA AAGGATCTCG ATCCGTCGGC TCCAACGATC TTGTCTCTGG CACCCAGCTG 
CCCAAGAATG GTATTCGTCC CACAAGCCAA GCACCCAAAT CTGTCTATTC AATTTCTGTG 
AATGGACAGA CCGTCAAAAT TTCGACGCCA AATCTTTGCA ATACAATGCT TCAGCATCAA 
AAGAAGGATT TATTTTCCAA ACCATCGGAC TTTGTGGTTC CTCAATTAGT GGTTAATCAA 
TTCAAAAACG GCTTAATCCC GTCCAATTCC CAGCGATCCA GTTCGCCGGC CAGGAACGTG 
ATACATTTGA AACCTAACAA CGACCATCCT TCAAAAATGG CTTTGTTCCT ACGTCAAGCA 
ATAACGGCAG ATTGTCCAAT AATTACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287181.1
CDS	549..2318
Translation	MWQFENKNATKMKTSQNEMPDKDKSFRRHSLSPRSLSAIFFHSTGLHLDDGVAQWLSINVKEDITNLLNEAGKYMRRIRDRRLQLSHIQHAVRMHDDLCYDIFFRLVHCDDCKMPPSQKVLKTVREAVTAEKKDELLVSYPESVQESVPEPVPETSLEPPPMHTGWLKVEQVLLKPSKRYPLSMEQQNFFELVTEACVGDLESRRVLALKAISTDPSLEELLPRLTKFIADAVAINVAQQNLPLLLYLMRMVRALLGNQRFSLLQYLHLLLPAVLSCLLAKQVCASPNSEDHWALREYSGNIMAHIVRQFDAADNGILPRVIGVYNKALLKKPLTTVFGAVIGLGKMGNHAVRACILPQLKYLSEHIDSHMAASNDSPSSSVDRQAVKYIRHRLMKMCSPVLMGIHQPPDLPEEFMERYGSLGTLMSDGVTVMRTKKQAVAAAKAEAELKLKNMATEHLNSRGKGSRSVGSNDLVSGTQLPKNGIRPTSQAPKSVYSISVNGQTVKISTPNLCNTMLQHQKKDLFSKPSDFVVPQLVVNQFKNGLIPSNSQRSSSPARNVIHLKPNNDHPSKMALFLRQAITADCPIIT

Target ORF information:

RefSeq Version	NM_001300252.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster meiosis I arrest (mia), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300252.1

ORF Insert Sequence:

ATGTGGCAGT TTGAAAACAA AAACGCAACT AAAATGAAAA CGTCTCAGAA TGAAATGCCC 
GACAAGGACA AAAGTTTTCG CAGACATTCT TTGTCGCCGC GTTCTCTATC CGCGATATTC 
TTCCACAGCA CTGGTCTGCA TCTGGACGAC GGAGTAGCAC AGTGGCTATC CATCAATGTT 
AAGGAAGACA TTACAAATCT GCTAAACGAG GCTGGAAAAT ACATGCGGCG CATCCGGGAT 
CGGCGATTGC AACTGTCTCA CATCCAGCAT GCCGTCCGAA TGCACGACGA CCTCTGCTAT 
GACATCTTTT TTAGGCTGGT CCATTGCGAC GATTGCAAAA TGCCTCCTTC CCAAAAAGTA 
TTAAAAACCG TCCGCGAAGC TGTAACCGCC GAAAAAAAGG ATGAACTTCT CGTTTCCTAC 
CCGGAATCCG TGCAGGAATC TGTGCCGGAA CCCGTGCCGG AGACTTCATT GGAGCCGCCT 
CCCATGCACA CCGGGTGGCT AAAGGTGGAG CAGGTGCTGC TAAAGCCCAG CAAGCGCTAT 
CCGCTGAGCA TGGAACAGCA GAACTTCTTC GAGTTGGTAA CCGAGGCCTG CGTAGGCGAC 
TTGGAATCCC GGCGCGTGCT TGCTCTCAAA GCCATCTCCA CTGATCCCTC GCTGGAGGAG 
TTGCTGCCCA GGTTGACCAA GTTCATTGCG GACGCAGTGG CTATCAACGT GGCCCAGCAG 
AACCTGCCGC TGCTCCTTTA CCTTATGCGC ATGGTCCGAG CTCTTTTGGG AAACCAGCGC 
TTCAGCCTAT TGCAATATCT CCACCTGCTC CTGCCAGCCG TTCTATCCTG CCTGCTGGCC 
AAACAAGTGT GCGCATCTCC GAACTCGGAG GATCACTGGG CCTTAAGGGA ATACTCCGGA 
AACATAATGG CCCACATAGT GCGCCAGTTC GACGCTGCCG ACAACGGCAT CCTGCCCAGA 
GTCATTGGTG TTTACAACAA GGCGCTATTG AAGAAGCCAC TGACAACGGT GTTTGGGGCC 
GTCATAGGCC TGGGAAAGAT GGGGAATCAT GCGGTCCGCG CATGCATTTT GCCTCAATTG 
AAGTACCTAT CTGAACACAT CGATTCACAC ATGGCCGCCA GTAATGACTC TCCCAGCTCC 
AGCGTAGACA GGCAAGCAGT TAAGTACATT CGTCATCGGT TGATGAAGAT GTGCTCTCCC 
GTGCTGATGG GCATACATCA ACCCCCTGAT CTGCCGGAAG AGTTTATGGA GAGATATGGA 
TCTTTGGGCA CGCTGATGAG CGATGGCGTC ACCGTGATGC GTACGAAGAA ACAAGCAGTG 
GCTGCAGCCA AGGCGGAAGC AGAACTGAAG TTGAAAAATA TGGCTACAGA ACACCTTAAC 
TCCAGAGGGA AAGGATCTCG ATCCGTCGGC TCCAACGATC TTGTCTCTGG CACCCAGCTG 
CCCAAGAATG GTATTCGTCC CACAAGCCAA GCACCCAAAT CTGTCTATTC AATTTCTGTG 
AATGGACAGA CCGTCAAAAT TTCGACGCCA AATCTTTGCA ATACAATGCT TCAGCATCAA 
AAGAAGGATT TATTTTCCAA ACCATCGGAC TTTGTGGTTC CTCAATTAGT GGTTAATCAA 
TTCAAAAACG GCTTAATCCC GTCCAATTCC CAGCGATCCA GTTCGCCGGC CAGGAACGTG 
ATACATTTGA AACCTAACAA CGACCATCCT TCAAAAATGG CTTTGTTCCT ACGTCAAGCA 
ATAACGGCAG ATTGTCCAAT AATTACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mia cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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