smash cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following smash gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smash cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa08056 NM_001259982.2
Latest version!
Drosophila melanogaster smallish (smash), transcript variant N, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OFa03850 NM_176400.1
Latest version!
Drosophila melanogaster smallish (smash), transcript variant J, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa03851 NM_141231.4
Latest version!
Drosophila melanogaster smallish (smash), transcript variant I, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa03849 NM_169015.3
Latest version!
Drosophila melanogaster smallish (smash), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa03849 NM_169014.3
Latest version!
Drosophila melanogaster smallish (smash), transcript variant E, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa03849 NM_176399.3
Latest version!
Drosophila melanogaster smallish (smash), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa08054 NM_001259984.2
Latest version!
Drosophila melanogaster smallish (smash), transcript variant P, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OFa08055 NM_001259983.2
Latest version!
Drosophila melanogaster smallish (smash), transcript variant O, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OFa08057 NM_001144532.3
Latest version!
Drosophila melanogaster smallish (smash), transcript variant H, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OFa08058 NM_001144533.3
Latest version!
Drosophila melanogaster smallish (smash), transcript variant M, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa08056
    Clone ID Related Accession (Same CDS sequence) NM_001259982.2
    Accession Version NM_001259982.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product smallish, isoform N
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001259982.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGGCCA CTGATCAGGA GGTCATGCTG GAGGGCAGGG AAGACCTCAT GTCCACATCG 
    GCGACATCGT CCACAAATGG CGGGGACACT AATGGTTGCG GCAAAAAATT GTCCGTAACC
    TCTCCGGATC CCACACCGTA CGTGACCAAG CAGCGAGTGA CGCGTCCAAC GCCTCCCGCT
    CCTTCAAAGA ATCCGATGCA ATTCGTCCAG ATCAAGCCCT GCAACCTCTA CCAGACCGCG
    CAGGAGCAGC TGAAGAAGGC CGAGGAGGTC AAGAAGCTGA AGGAGGTGAA GAAGGAGGAG
    CCAGAGGAGT GGCAGAACAA CCTTGACAAT TGGAAATCAT CGAGACGAAA ACGTGTCGAA
    CACATCATCG ATCGAGCGGT GGAGACCAAG AAACTCGAGC TGGAGGAGCA TGATCGGTTG
    CGCCGAAAGT CGAAAACATT CACCGAAATG ATGGAGGAGA GGGCTGAAAG GGGCGGACCT
    CGGGGGCGTG CCAAGCTGGC TTCCCTGGCC GTATACAACG AGGATGAGAC GAACGACCTG
    AGTGATTTGG GGATCGGAAC CAGCAGTGCC AGCGGAAAGA GCAGCCTGTC CGGGGACTAT
    GACAATAACA GTGTTATGAG CGACCATGCG GCGGAGCTGG ATAAGGCGAT TGGAGCAGCT
    AGCTCTGTAG CTGCTGGAGT AACCGCCCAA AACGTGGATG AGCAGCTGAA TCACATAAAC
    CGCAACGGTA GTAATGGGAA CCACGGGAAC GGAGCGGCTG TTGGCCAGAC AGGACCATCA
    AATTCCTCCA AGTCAGCAGG GCGGCAATAC ATCTCCTCGC CAGGATACGA TACGTCCTCC
    AGTAACGCGC AAGCCAGTTC TCCGGATCTC TGTGAATACA CCTACGAAGG AGCCATCCAG
    GACTACAAGC AAAGAGTTCA ACGGGCCAGC AGCAATGGCA ATGGAAATGC CAACGGCAAG
    TTGATTGGAG AACATATTGC TTATCCCACG AGGCGAGGCT CTAAAATTGA GGATCGATTG
    AGTGGTTTTG AGGTGACATC GCCAAGCGAC ACGCAGGAGG GTGTAGAGAA GCAAAAGGTG
    GACGTGCCCA AGGTTGACAT ATCCAAACGC AAGGAGATCT TCGAGCAGGC CAAGGCGGAA
    GTCTCAAACG GTGGTGCTCC AGCTGCCCCA AAACTCGTAT TTCGTGATGG TCTTACCAAT
    GGAAATTCAG CTACCACTGC TCCCAAATCG GAGGTGAGGC GTCTTTCTGG TGACATCTCC
    AGCATTCGAG ATCGCATGCA GAGTTTGGAA CAGCAGCGGA AGGCTTTCAG TTCCAGCAAG
    AGTGTGGATG TGCCGGTGCC GCCCCTTAAA CAGCGTCTGA ATAGTCTGCA GCAGTCCGTT
    GCCAAAGAGG AGCAGAAGAA GCCACCGTTG GTGGCGCTCA TCGATGCCAG ACAACTGGAG
    ATCATGAGGG GAGAAGAGGA ACGTATGCGC CAGCAGCAAC AAAAAGAGAA GCATTCACCA
    AGCCAAACTA CATTGTGTGC ACCTAAGCCA GCTCCTCCTG CCCTTATTAT TGAGGAACCG
    CCGGTGGCAG ACACCAACGA CGACAGCGGC ATTCAGGAGG ACACCGCTGA TGAACTGCAG
    CAGCAGCAGC AACTCAACGC AGCCATCGCC GCCTTGGCGC TCGAGGAGCG CCAGTTGGAG
    GAAGCAGCCA ATGCGGTCAA CCAAATTGAA GCGGAGTTCG ATGAGCTGAC TGATCTGCAT
    CCCTCGCCAT TGCCACCATC TCCAGCTCTT CCGGCGGTTC AACAAAGCCT CCAACAACCG
    ACAGTCGCTC AGGTTCCAGC ACAAGCGGTC AATCAGGCGG CTGCTCCTCC ATTGCGGGAC
    ATGGAATTTA GTTTACATAG TTTACATGCT TACTGCCAGC AAAAGTTGTT GCAAGTATAC
    AATTTCCGAG ATCAACGAAG CTCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246911.1
    CDS763..2709
    Translation

    Target ORF information:

    RefSeq Version NM_001259982.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster smallish (smash), transcript variant N, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259982.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGGCCA CTGATCAGGA GGTCATGCTG GAGGGCAGGG AAGACCTCAT GTCCACATCG 
    GCGACATCGT CCACAAATGG CGGGGACACT AATGGTTGCG GCAAAAAATT GTCCGTAACC
    TCTCCGGATC CCACACCGTA CGTGACCAAG CAGCGAGTGA CGCGTCCAAC GCCTCCCGCT
    CCTTCAAAGA ATCCGATGCA ATTCGTCCAG ATCAAGCCCT GCAACCTCTA CCAGACCGCG
    CAGGAGCAGC TGAAGAAGGC CGAGGAGGTC AAGAAGCTGA AGGAGGTGAA GAAGGAGGAG
    CCAGAGGAGT GGCAGAACAA CCTTGACAAT TGGAAATCAT CGAGACGAAA ACGTGTCGAA
    CACATCATCG ATCGAGCGGT GGAGACCAAG AAACTCGAGC TGGAGGAGCA TGATCGGTTG
    CGCCGAAAGT CGAAAACATT CACCGAAATG ATGGAGGAGA GGGCTGAAAG GGGCGGACCT
    CGGGGGCGTG CCAAGCTGGC TTCCCTGGCC GTATACAACG AGGATGAGAC GAACGACCTG
    AGTGATTTGG GGATCGGAAC CAGCAGTGCC AGCGGAAAGA GCAGCCTGTC CGGGGACTAT
    GACAATAACA GTGTTATGAG CGACCATGCG GCGGAGCTGG ATAAGGCGAT TGGAGCAGCT
    AGCTCTGTAG CTGCTGGAGT AACCGCCCAA AACGTGGATG AGCAGCTGAA TCACATAAAC
    CGCAACGGTA GTAATGGGAA CCACGGGAAC GGAGCGGCTG TTGGCCAGAC AGGACCATCA
    AATTCCTCCA AGTCAGCAGG GCGGCAATAC ATCTCCTCGC CAGGATACGA TACGTCCTCC
    AGTAACGCGC AAGCCAGTTC TCCGGATCTC TGTGAATACA CCTACGAAGG AGCCATCCAG
    GACTACAAGC AAAGAGTTCA ACGGGCCAGC AGCAATGGCA ATGGAAATGC CAACGGCAAG
    TTGATTGGAG AACATATTGC TTATCCCACG AGGCGAGGCT CTAAAATTGA GGATCGATTG
    AGTGGTTTTG AGGTGACATC GCCAAGCGAC ACGCAGGAGG GTGTAGAGAA GCAAAAGGTG
    GACGTGCCCA AGGTTGACAT ATCCAAACGC AAGGAGATCT TCGAGCAGGC CAAGGCGGAA
    GTCTCAAACG GTGGTGCTCC AGCTGCCCCA AAACTCGTAT TTCGTGATGG TCTTACCAAT
    GGAAATTCAG CTACCACTGC TCCCAAATCG GAGGTGAGGC GTCTTTCTGG TGACATCTCC
    AGCATTCGAG ATCGCATGCA GAGTTTGGAA CAGCAGCGGA AGGCTTTCAG TTCCAGCAAG
    AGTGTGGATG TGCCGGTGCC GCCCCTTAAA CAGCGTCTGA ATAGTCTGCA GCAGTCCGTT
    GCCAAAGAGG AGCAGAAGAA GCCACCGTTG GTGGCGCTCA TCGATGCCAG ACAACTGGAG
    ATCATGAGGG GAGAAGAGGA ACGTATGCGC CAGCAGCAAC AAAAAGAGAA GCATTCACCA
    AGCCAAACTA CATTGTGTGC ACCTAAGCCA GCTCCTCCTG CCCTTATTAT TGAGGAACCG
    CCGGTGGCAG ACACCAACGA CGACAGCGGC ATTCAGGAGG ACACCGCTGA TGAACTGCAG
    CAGCAGCAGC AACTCAACGC AGCCATCGCC GCCTTGGCGC TCGAGGAGCG CCAGTTGGAG
    GAAGCAGCCA ATGCGGTCAA CCAAATTGAA GCGGAGTTCG ATGAGCTGAC TGATCTGCAT
    CCCTCGCCAT TGCCACCATC TCCAGCTCTT CCGGCGGTTC AACAAAGCCT CCAACAACCG
    ACAGTCGCTC AGGTTCCAGC ACAAGCGGTC AATCAGGCGG CTGCTCCTCC ATTGCGGGAC
    ATGGAATTTA GTTTACATAG TTTACATGCT TACTGCCAGC AAAAGTTGTT GCAAGTATAC
    AATTTCCGAG ATCAACGAAG CTCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa03850
    Clone ID Related Accession (Same CDS sequence) NM_176400.1
    Accession Version NM_176400.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2550bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product smallish, isoform J
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGACAAGG CACAACATCC ACAGAAGCGG GAACAGAAGG AGGAGCTGGG AGGTAAGCCG 
    GAGGAGAAGG AGCCAATTTG CGAGGAGCAG CACGACGAAG AGTCCAACGA GCAGGGGGAA
    ACACACAACG AGGGTGGGGA AATGCCAAAT AAAACCGATA AGCAAATGCA GGGGGGACGA
    GAGCCCATCT ATGAGAATGT CAGCTCGACT ATATCTATGC ATGAAGTAGA TAATGAACAG
    ATAGCAACTA TGACGATAAC CACACAGACC CAAGCGACGC GAATAAGTCA CAGGAGAAGC
    ATTCCAAGCC AGCAAACCAG CAACAGTAAC GAGAATAATC AAAGCACTAA TCAAACCAGC
    AACAGCAGGA ATCAAAAAGA GAGCAGCACA GCAGCAACAT CGGCAGCGAA CCCCGTGCCA
    CCAACAACAC CGGGGGAACA AGCAGAGCCC TACTACCAAG TGCCGAAGGC CACGGAGCCC
    TACTACGATG CCCCCAAGCA TTTAAGGCCG GTGCCGGTAT ACGAAAATGT CGAGATCTTT
    TACTCGGGCC TGGAGATTAG TCAGGGATCT GTGGGAGCGC CAGTAGGGCT GATGGAGCCA
    CCCAAGGAAA AGCCACCACC ACCGCCCACC GAGAGTCCGA TTCCGCTGGA CGAGGGCCAT
    GACGACGAGC TGGATGGACT GGGCAACAGT CTGGGCCACA ACACAGACAC CTGGTCATCG
    GACAACACCT ACGAGACCAT ATCGAACGGC ACTCGCCGGC ACCTTCAGGG ACAACCGGAA
    CCCGCCCAAC CCTCGCCGCC CATCAAGAGG ATGAACTCGA CCAAACGGAT CAAGAAGGAG
    TTGCGCAACA AGCGCTCCAG CTTCCTGGGC ATCGAAAACG ACGGCGATCT TGACGACATG
    GAGACCTATC TCGAGCTAAC AGTGGCCCCG CCTCCGGATA TGGCGCAGCT CTTGCAGGAG
    GAGCGGCGAC TGGAGAAGCA GCTGTACATC AAGGCGGGAC TCTGCGACAG TTCCGACACA
    GGTGAAAGCC GCGATTCTGG AGTTTCTGAA AACCACTCTC GCCAGAGCAG TGAGCACTAC
    ACGAACTCCT CTGAGGAGAA CGATACACAG TCAGAGGCCA CACCGCCGCC ATTGCCTCCA
    CCACCATCGA CCGCCCAAGT GGGTGAAGTC ATATACCAAA ATGAGAGCCT CCTGGCTGCC
    CAGACGCCGC TTCTCCAGAC GGTAAAAGGA AACTCCGCCG AGTCCTGGAC CGAATCAGCA
    ACGGCCGCAG CGGCGGCCAC GCAATCATTG AGCGAAGCCA CAGCAACGGC CAATGCCAAG
    ATGCAGTCCA TCGAGGATAA GATCCGGGAG CAGGGAGAGA TGTTGCGGGT GGAACGCGAG
    CTACTGCACT TCTCGCAAGA GGAACTGAAA CGGCAACGTG AAAATCTTGT GCTTCGAGAA
    AATATTGCTC GACGAGAGTT GCAACACGGA GCCAAGATGC TGATGTCGAA CAACCGGCGC
    TCACTGCAGG ATCTGCACCA CGGTCTAGGA ATCGGAAACG GAATGCTCAG TGCCTTCCAG
    CCACCGCAAC ACCACCAGGT TCAAATGCCA CCACCGCATC CGCAACAGAT TTACGCCAAT
    GTGCCGCAGC AGCAGCAACA GATGGTTCTG CATGCATATC ACCAGATGGA CACGGACTAC
    CGCAAGTCCA TGTCGGATCT AAACGAGTTC TCCAACTGCC TGATACTGCC GCCAACGCCG
    CCCACAAAGC CATTGAGGGC TATGCAATTG AACGCCAATG GCCATGGCCT GGAGCCAGAT
    TACGCTGTTA GCACGAGGCA GCGCCAACCA CCGGCTCCGT ACGGTGGCTC TCTGGTAAAG
    ATTGCTGGAG CACCAATGCC GCCACGGCTC CCCGGTCAAG GATATCCCAT TCAAGCGCCG
    ACAGCGCCAG CATACCATCA CCAGTCGGCC CAAAACTTAA GCAATATGTC CAGAAACACA
    TTGCTCGCTT TGAGTGCCAC CCCCAAACCA AAATACGCGG ACGGATGGGT GCAGGTGCAG
    CAGCGGAAGA GTTATGACAG CCAACAGACC AGCGATGCGG CTTGGCTGGC CGCTCAGCAG
    CAAAAGCGCA AATCCATGCC TGACTACGGC GGAGCACTCT ACAATAACAA CCACTGGCTA
    CTGCAGGAGG CGGAGCAGCG ACGCATCGAG CAACTTAATC GGCGATCTAT ACCAGCCAGC
    AAGTCGATGG GCAAACCGCT GCCCGATTCC ATCATACAAA CTCTTACGGA GCGGGTCCAG
    AGCAAAGGAA TCGGCGATCG AAAGCGCTTC GATAGCAACG GGAACTATAG CCAGGTAAAT
    GGCAACAACA TCTACCAGCA GCAACAGCAG CAGAAGAATG TGACGAATGG CAGCAGCATC
    AATGGCAACA GCAGCCAGGG GCAGGAGAAG GTGCTCAGCG TTAGTGGCAA GAAGAAGTGC
    TCCCATTGCG GCGACGAATT AGATCTGGGC TGCACTCACG GCAATTGTCG GCAGGTGCAC
    AAAGCGCTAA AATATATTGT TTGGGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788577.1
    CDS281..2830
    Translation

    Target ORF information:

    RefSeq Version NM_176400.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster smallish (smash), transcript variant J, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176400.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGACAAGG CACAACATCC ACAGAAGCGG GAACAGAAGG AGGAGCTGGG AGGTAAGCCG 
    GAGGAGAAGG AGCCAATTTG CGAGGAGCAG CACGACGAAG AGTCCAACGA GCAGGGGGAA
    ACACACAACG AGGGTGGGGA AATGCCAAAT AAAACCGATA AGCAAATGCA GGGGGGACGA
    GAGCCCATCT ATGAGAATGT CAGCTCGACT ATATCTATGC ATGAAGTAGA TAATGAACAG
    ATAGCAACTA TGACGATAAC CACACAGACC CAAGCGACGC GAATAAGTCA CAGGAGAAGC
    ATTCCAAGCC AGCAAACCAG CAACAGTAAC GAGAATAATC AAAGCACTAA TCAAACCAGC
    AACAGCAGGA ATCAAAAAGA GAGCAGCACA GCAGCAACAT CGGCAGCGAA CCCCGTGCCA
    CCAACAACAC CGGGGGAACA AGCAGAGCCC TACTACCAAG TGCCGAAGGC CACGGAGCCC
    TACTACGATG CCCCCAAGCA TTTAAGGCCG GTGCCGGTAT ACGAAAATGT CGAGATCTTT
    TACTCGGGCC TGGAGATTAG TCAGGGATCT GTGGGAGCGC CAGTAGGGCT GATGGAGCCA
    CCCAAGGAAA AGCCACCACC ACCGCCCACC GAGAGTCCGA TTCCGCTGGA CGAGGGCCAT
    GACGACGAGC TGGATGGACT GGGCAACAGT CTGGGCCACA ACACAGACAC CTGGTCATCG
    GACAACACCT ACGAGACCAT ATCGAACGGC ACTCGCCGGC ACCTTCAGGG ACAACCGGAA
    CCCGCCCAAC CCTCGCCGCC CATCAAGAGG ATGAACTCGA CCAAACGGAT CAAGAAGGAG
    TTGCGCAACA AGCGCTCCAG CTTCCTGGGC ATCGAAAACG ACGGCGATCT TGACGACATG
    GAGACCTATC TCGAGCTAAC AGTGGCCCCG CCTCCGGATA TGGCGCAGCT CTTGCAGGAG
    GAGCGGCGAC TGGAGAAGCA GCTGTACATC AAGGCGGGAC TCTGCGACAG TTCCGACACA
    GGTGAAAGCC GCGATTCTGG AGTTTCTGAA AACCACTCTC GCCAGAGCAG TGAGCACTAC
    ACGAACTCCT CTGAGGAGAA CGATACACAG TCAGAGGCCA CACCGCCGCC ATTGCCTCCA
    CCACCATCGA CCGCCCAAGT GGGTGAAGTC ATATACCAAA ATGAGAGCCT CCTGGCTGCC
    CAGACGCCGC TTCTCCAGAC GGTAAAAGGA AACTCCGCCG AGTCCTGGAC CGAATCAGCA
    ACGGCCGCAG CGGCGGCCAC GCAATCATTG AGCGAAGCCA CAGCAACGGC CAATGCCAAG
    ATGCAGTCCA TCGAGGATAA GATCCGGGAG CAGGGAGAGA TGTTGCGGGT GGAACGCGAG
    CTACTGCACT TCTCGCAAGA GGAACTGAAA CGGCAACGTG AAAATCTTGT GCTTCGAGAA
    AATATTGCTC GACGAGAGTT GCAACACGGA GCCAAGATGC TGATGTCGAA CAACCGGCGC
    TCACTGCAGG ATCTGCACCA CGGTCTAGGA ATCGGAAACG GAATGCTCAG TGCCTTCCAG
    CCACCGCAAC ACCACCAGGT TCAAATGCCA CCACCGCATC CGCAACAGAT TTACGCCAAT
    GTGCCGCAGC AGCAGCAACA GATGGTTCTG CATGCATATC ACCAGATGGA CACGGACTAC
    CGCAAGTCCA TGTCGGATCT AAACGAGTTC TCCAACTGCC TGATACTGCC GCCAACGCCG
    CCCACAAAGC CATTGAGGGC TATGCAATTG AACGCCAATG GCCATGGCCT GGAGCCAGAT
    TACGCTGTTA GCACGAGGCA GCGCCAACCA CCGGCTCCGT ACGGTGGCTC TCTGGTAAAG
    ATTGCTGGAG CACCAATGCC GCCACGGCTC CCCGGTCAAG GATATCCCAT TCAAGCGCCG
    ACAGCGCCAG CATACCATCA CCAGTCGGCC CAAAACTTAA GCAATATGTC CAGAAACACA
    TTGCTCGCTT TGAGTGCCAC CCCCAAACCA AAATACGCGG ACGGATGGGT GCAGGTGCAG
    CAGCGGAAGA GTTATGACAG CCAACAGACC AGCGATGCGG CTTGGCTGGC CGCTCAGCAG
    CAAAAGCGCA AATCCATGCC TGACTACGGC GGAGCACTCT ACAATAACAA CCACTGGCTA
    CTGCAGGAGG CGGAGCAGCG ACGCATCGAG CAACTTAATC GGCGATCTAT ACCAGCCAGC
    AAGTCGATGG GCAAACCGCT GCCCGATTCC ATCATACAAA CTCTTACGGA GCGGGTCCAG
    AGCAAAGGAA TCGGCGATCG AAAGCGCTTC GATAGCAACG GGAACTATAG CCAGGTAAAT
    GGCAACAACA TCTACCAGCA GCAACAGCAG CAGAAGAATG TGACGAATGG CAGCAGCATC
    AATGGCAACA GCAGCCAGGG GCAGGAGAAG GTGCTCAGCG TTAGTGGCAA GAAGAAGTGC
    TCCCATTGCG GCGACGAATT AGATCTGGGC TGCACTCACG GCAATTGTCG GCAGGTGCAC
    AAAGCGCTAA AATATATTGT TTGGGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa03851
    Clone ID Related Accession (Same CDS sequence) NM_141231.4
    Accession Version NM_141231.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2670bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product smallish, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_141231.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACAAGG CACAACATCC ACAGAAGCGG GAACAGAAGG AGGAGCTGGG AGGTAAGCCG 
    GAGGAGAAGG AGCCAATTTG CGAGGAGCAG CACGACGAAG AGTCCAACGA GCAGGGGGAA
    ACACACAACG AGGGTGGGGA AATGCCAAAT AAAACCGATA AGCAAATGCA GGGGGGACGA
    GAGCCCATCT ATGAGAATGT CAGCTCGACT ATATCTATGC ATGAAGTAGA TAATGAACAG
    ATAGCAACTA TGACGATAAC CACACAGACC CAAGCGACGC GAATAAGTCA CAGGAGAAGC
    ATTCCAAGCC AGCAAACCAG CAACAGTAAC GAGAATAATC AAAGCACTAA TCAAACCAGC
    AACAGCAGGA ATCAAAAAGA GAGCAGCACA GCAGCAACAT CGGCAGCGAA CCCCGTGCCA
    CCAACAACAC CGGGGGAACA AGCAGAGCCC TACTACCAAG TGCCGAAGGC CACGGAGCCC
    TACTACGATG CCCCCAAGCA TTTAAGGCCG GTGCCGGTAT ACGAAAATGT CGAGATCTTT
    TACTCGGGCC TGGAGATTAG TCAGGGATCT GTGGGAGCGC CAGTAGGGCT GATGGAGCCA
    CCCAAGGAAA AGCCACCACC ACCGCCCACC GAGAGTCCGA TTCCGCTGGA CGAGGGCCAT
    GACGACGAGC TGGATGGACT GGGCAACAGT CTGGGCCACA ACACAGACAC CTGGTCATCG
    GACAACACCT ACGAGACCAT ATCGAACGGC ACTCGCCGGC ACCTTCAGGG ACAACCGGAA
    CCCGCCCAAC CCTCGCCGCC CATCAAGAGG ATGAACTCGA CCAAACGGAT CAAGAAGGAG
    TTGCGCAACA AGCGCTCCAG CTTCCTGGGC ATCGAAAACG ACGGCGATCT TGACGACATG
    GAGACCTATC TCGAGCTAAC AGTGGCCCCG CCTCCGGATA TGGCGCAGCT CTTGCAGGAG
    GAGCGGCGAC TGGAGAAGCA GCTGTACATC AAGGCGGGAC TCTGCGACAG TTCCGACACA
    GGTGAAAGCC GCGATTCTGG AGTTTCTGAA AACCACTCTC GCCAGAGCAG TGAGCACTAC
    ACGAACTCCT CTGAGGAGAA CGATACACAG TCAGAGGCCA CACCGCCGCC ATTGCCTCCA
    CCACCATCGA CCGCCCAAGT GGGTGAAGTC ATATACCAAA ATGAGAGCCT CCTGGCTGCC
    CAGACGCCGC TTCTCCAGAC GGTAAAAGGA AACTCCGCCG AGTCCTGGAC CGAATCAGCA
    ACGGCCGCAG CGGCGGCCAC GCAATCATTG AGCGAAGCCA CAGCAACGGC CAATGCCAAG
    ATGCAGTCCA TCGAGGATAA GATCCGGGAG CAGGGAGAGA TGTTGCGGGT GGAACGCGAG
    CTACTGCACT TCTCGCAAGA GGAACTGAAA CGGCAACGTG AAAATCTTGT GCTTCGAGAA
    AATATTGCTC GACGAGAGTT GCAACACGGA GCCAAGATGC TGATGTCGAA CAACCGGCGC
    TCACTGCAGG ATCTGCACCA CGGTCTAGGA ATCGGAAACG GAATGCTCAG TGCCTTCCAG
    CCACCGCAAC ACCACCAGGT TCAAATGCCA CCACCGCATC CGCAACAGAT TTACGCCAAT
    GTGCCGCAGC AGCAGCAACA GATGGTTCTG CATGCATATC ACCAGATGGA CACGGACTAC
    CGCAAGTCCA TGTCGGATCT AAACGAGTTC TCCAACTGCC TGATACTGCC GCCAACGCCG
    CCCACAAAGC CATTGAGGGC TATGCAATTG AACGCCAATG GCCATGGCCT GGAGCCAGAT
    TACGCTGTTA GCACGAGGCA GCGCCAACCA CCGGCTCCGT ACGGTGGCTC TCTGGTAAAG
    ATTGCTGGAG CACCAATGCC GCCACGGCTC CCCGGTCAAG GATATCCCAT TCAAGCGCCG
    ACAGCGCCAG CATACCATCA CCAGTCGGCC CAAAACTTAA GCAATATGTC CAGAAACACA
    TTGCTCGCTT TGAGTGCCAC CCCCAAACCA AAATACGCGG ACGGATGGGT GCAGGTGCAG
    CAGCGGAAGA GTTATGACAG CCAACAGACC AGCGATGCGG CTTGGCTGGC CGCTCAGCAG
    CAAAAGCGCA AATCCATGCC TGACTACGGC GGAGCACTCT ACAATAACAA CCACTGGCTA
    CTGCAGGAGG CGGAGCAGCG ACGCATCGAG CAACTTAATC GGCGATCTAT ACCAGCCAGC
    AAGTCGATGG GCAAACCGCT GCCCGATTCC ATCATACAAA CTCTTACGGA GCGGGTCCAG
    AGCAAAGGAA TCGGCGATCG AAAGCGCTTC GATAGCAACG GGAACTATAG CCAGGTAAAT
    GGCAACAACA TCTACCAGCA GCAACAGCAG CAGAAGAATG TGACGAATGG CAGCAGCATC
    AATGGCAACA GCAGCCAGGG GCAGGAGAAG GTGCTCAGCG TTAGTGGCAA GAAGAAGTGC
    TCCCATTGCG GCGACGAATT AGGTCGCGGC GCTGCCATGA TCATCGAATC GCTTCTGCTG
    TTCTACCACA TCAACTGCTT CAAGTGTTGC GTCTGCCACG TCCAGCTGGG AGATGGCCTC
    AATGGAACCG ATGTCCGGGT GCGAAACCAC AAGCTGCATT GCCAAAACTG CTACTCCAGC
    GATGACGGCA TTAAGTTCAG CTGCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_649488.2
    CDS281..2950
    Translation

    Target ORF information:

    RefSeq Version NM_141231.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster smallish (smash), transcript variant I, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_141231.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACAAGG CACAACATCC ACAGAAGCGG GAACAGAAGG AGGAGCTGGG AGGTAAGCCG 
    GAGGAGAAGG AGCCAATTTG CGAGGAGCAG CACGACGAAG AGTCCAACGA GCAGGGGGAA
    ACACACAACG AGGGTGGGGA AATGCCAAAT AAAACCGATA AGCAAATGCA GGGGGGACGA
    GAGCCCATCT ATGAGAATGT CAGCTCGACT ATATCTATGC ATGAAGTAGA TAATGAACAG
    ATAGCAACTA TGACGATAAC CACACAGACC CAAGCGACGC GAATAAGTCA CAGGAGAAGC
    ATTCCAAGCC AGCAAACCAG CAACAGTAAC GAGAATAATC AAAGCACTAA TCAAACCAGC
    AACAGCAGGA ATCAAAAAGA GAGCAGCACA GCAGCAACAT CGGCAGCGAA CCCCGTGCCA
    CCAACAACAC CGGGGGAACA AGCAGAGCCC TACTACCAAG TGCCGAAGGC CACGGAGCCC
    TACTACGATG CCCCCAAGCA TTTAAGGCCG GTGCCGGTAT ACGAAAATGT CGAGATCTTT
    TACTCGGGCC TGGAGATTAG TCAGGGATCT GTGGGAGCGC CAGTAGGGCT GATGGAGCCA
    CCCAAGGAAA AGCCACCACC ACCGCCCACC GAGAGTCCGA TTCCGCTGGA CGAGGGCCAT
    GACGACGAGC TGGATGGACT GGGCAACAGT CTGGGCCACA ACACAGACAC CTGGTCATCG
    GACAACACCT ACGAGACCAT ATCGAACGGC ACTCGCCGGC ACCTTCAGGG ACAACCGGAA
    CCCGCCCAAC CCTCGCCGCC CATCAAGAGG ATGAACTCGA CCAAACGGAT CAAGAAGGAG
    TTGCGCAACA AGCGCTCCAG CTTCCTGGGC ATCGAAAACG ACGGCGATCT TGACGACATG
    GAGACCTATC TCGAGCTAAC AGTGGCCCCG CCTCCGGATA TGGCGCAGCT CTTGCAGGAG
    GAGCGGCGAC TGGAGAAGCA GCTGTACATC AAGGCGGGAC TCTGCGACAG TTCCGACACA
    GGTGAAAGCC GCGATTCTGG AGTTTCTGAA AACCACTCTC GCCAGAGCAG TGAGCACTAC
    ACGAACTCCT CTGAGGAGAA CGATACACAG TCAGAGGCCA CACCGCCGCC ATTGCCTCCA
    CCACCATCGA CCGCCCAAGT GGGTGAAGTC ATATACCAAA ATGAGAGCCT CCTGGCTGCC
    CAGACGCCGC TTCTCCAGAC GGTAAAAGGA AACTCCGCCG AGTCCTGGAC CGAATCAGCA
    ACGGCCGCAG CGGCGGCCAC GCAATCATTG AGCGAAGCCA CAGCAACGGC CAATGCCAAG
    ATGCAGTCCA TCGAGGATAA GATCCGGGAG CAGGGAGAGA TGTTGCGGGT GGAACGCGAG
    CTACTGCACT TCTCGCAAGA GGAACTGAAA CGGCAACGTG AAAATCTTGT GCTTCGAGAA
    AATATTGCTC GACGAGAGTT GCAACACGGA GCCAAGATGC TGATGTCGAA CAACCGGCGC
    TCACTGCAGG ATCTGCACCA CGGTCTAGGA ATCGGAAACG GAATGCTCAG TGCCTTCCAG
    CCACCGCAAC ACCACCAGGT TCAAATGCCA CCACCGCATC CGCAACAGAT TTACGCCAAT
    GTGCCGCAGC AGCAGCAACA GATGGTTCTG CATGCATATC ACCAGATGGA CACGGACTAC
    CGCAAGTCCA TGTCGGATCT AAACGAGTTC TCCAACTGCC TGATACTGCC GCCAACGCCG
    CCCACAAAGC CATTGAGGGC TATGCAATTG AACGCCAATG GCCATGGCCT GGAGCCAGAT
    TACGCTGTTA GCACGAGGCA GCGCCAACCA CCGGCTCCGT ACGGTGGCTC TCTGGTAAAG
    ATTGCTGGAG CACCAATGCC GCCACGGCTC CCCGGTCAAG GATATCCCAT TCAAGCGCCG
    ACAGCGCCAG CATACCATCA CCAGTCGGCC CAAAACTTAA GCAATATGTC CAGAAACACA
    TTGCTCGCTT TGAGTGCCAC CCCCAAACCA AAATACGCGG ACGGATGGGT GCAGGTGCAG
    CAGCGGAAGA GTTATGACAG CCAACAGACC AGCGATGCGG CTTGGCTGGC CGCTCAGCAG
    CAAAAGCGCA AATCCATGCC TGACTACGGC GGAGCACTCT ACAATAACAA CCACTGGCTA
    CTGCAGGAGG CGGAGCAGCG ACGCATCGAG CAACTTAATC GGCGATCTAT ACCAGCCAGC
    AAGTCGATGG GCAAACCGCT GCCCGATTCC ATCATACAAA CTCTTACGGA GCGGGTCCAG
    AGCAAAGGAA TCGGCGATCG AAAGCGCTTC GATAGCAACG GGAACTATAG CCAGGTAAAT
    GGCAACAACA TCTACCAGCA GCAACAGCAG CAGAAGAATG TGACGAATGG CAGCAGCATC
    AATGGCAACA GCAGCCAGGG GCAGGAGAAG GTGCTCAGCG TTAGTGGCAA GAAGAAGTGC
    TCCCATTGCG GCGACGAATT AGGTCGCGGC GCTGCCATGA TCATCGAATC GCTTCTGCTG
    TTCTACCACA TCAACTGCTT CAAGTGTTGC GTCTGCCACG TCCAGCTGGG AGATGGCCTC
    AATGGAACCG ATGTCCGGGT GCGAAACCAC AAGCTGCATT GCCAAAACTG CTACTCCAGC
    GATGACGGCA TTAAGTTCAG CTGCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa03849
    Clone ID Related Accession (Same CDS sequence) NM_169014.3 , NM_176399.3 , NM_169015.3
    Accession Version NM_169014.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4119bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product smallish, isoform E
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169014.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGGCCA CTGATCAGGA GGTCATGCTG GAGGGCAGGG AAGACCTCAT GTCCACATCG 
    GCGACATCGT CCACAAATGG CGGGGACACT AATGGTTGCG GCAAAAAATT GTCCGTAACC
    TCTCCGGATC CCACACCGTA CGTGACCAAG CAGCGAGTGA CGCGTCCAAC GCCTCCCGCT
    CCTTCAAAGA ATCCGATGCA ATTCGTCCAG ATCAAGCCCT GCAACCTCTA CCAGACCGCG
    CAGGAGCAGC TGAAGAAGGC CGAGGAGGTC AAGAAGCTGA AGGAGGTGAA GAAGGAGGAG
    CCAGAGGAGT GGCAGAACAA CCTTGACAAT TGGAAATCAT CGAGACGAAA ACGTGTCGAA
    CACATCATCG ATCGAGCGGT GGAGACCAAG AAACTCGAGC TGGAGGAGCA TGATCGGTTG
    CGCCGAAAGT CGAAAACATT CACCGAAATG ATGGAGGAGA GGGCTGAAAG GGGCGGACCT
    CGGGGGCGTG CCAAGCTGGC TTCCCTGGCC GTATACAACG AGGATGAGAC GAACGACCTG
    AGTGATTTGG GGATCGGAAC CAGCAGTGCC AGCGGAAAGA GCAGCCTGTC CGGGGACTAT
    GACAATAACA GTGTTATGAG CGACCATGCG GCGGAGCTGG ATAAGGCGAT TGGAGCAGCT
    AGCTCTGTAG CTGCTGGAGT AACCGCCCAA AACGTGGATG AGCAGCTGAA TCACATAAAC
    CGCAACGGTA GTAATGGGAA CCACGGGAAC GGAGCGGCTG TTGGCCAGAC AGGACCATCA
    AATTCCTCCA AGTCAGCAGG GCGGCAATAC ATCTCCTCGC CAGGATACGA TACGTCCTCC
    AGTAACGCGC AAGCCAGTTC TCCGGATCTC TGTGAATACA CCTACGAAGG AGCCATCCAG
    GACTACAAGC AAAGAGTTCA ACGGGCCAGC AGCAATGGCA ATGGAAATGC CAACGGCAAG
    TTGATTGGAG AACATATTGC TTATCCCACG AGGCGAGGCT CTAAAATTGA GGATCGATTG
    AGTGGTTTTG AGGTGACATC GCCAAGCGAC ACGCAGGAGG GTGTAGAGAA GCAAAAGGTG
    GACGTGCCCA AGGTTGACAT ATCCAAACGC AAGGAGATCT TCGAGCAGGC CAAGGCGGAA
    GTCTCAAACG GTGGTGCTCC AGCTGCCCCA AAACTCGTAT TTCGTGATGG TCTTACCAAT
    GGAAATTCAG CTACCACTGC TCCCAAATCG GAGGTGAGGC GTCTTTCTGG TGACATCTCC
    AGCATTCGAG ATCGCATGCA GAGTTTGGAA CAGCAGCGGA AGGCTTTCAG TTCCAGCAAG
    AGTGTGGATG TGCCGGTGCC GCCCCTTAAA CAGCGTCTGA ATAGTCTGCA GCAGTCCGTT
    GCCAAAGAGG AGCAGAAGAA GCCACCGTTG GTGGCGCTCA TCGATGCCAG ACAACTGGAG
    ATCATGAGGG GAGAAGAGGA ACGTATGCGC CAGCAGCAAC AAAAAGAGAA GCATTCACCA
    AGCCAAACTA CATTGTGTGC ACCTAAGCCA GCTCCTCCTG CCCTTATTAT TGAGGAACCG
    CCGGTGGCAG ACACCAACGA CGACAGCGGC ATTCAGGAGG ACACCGCTGA TGAACTGCAG
    CAGCAGCAGC AACTCAACGC AGCCATCGCC GCCTTGGCGC TCGAGGAGCG CCAGTTGGAG
    GAAGCAGCCA ATGCGGTCAA CCAAATTGAA GCGGAGTTCG ATGAGCTGAC TGATCTGCAT
    CCCTCGCCAT TGCCACCATC TCCAGCTCTT CCGGCGGTTC AACAAAGCCT CCAACAACCG
    ACAGTCGCTC AGGTTCCAGC ACAAGCGGTC AATCAGGCGG CTGCTCCTCC ATTGCGGGAC
    ATGGAATTTA GTGTAAGTTG TACTAACAAT TTTATCGAAC CCGCTAAGAT AAGTCCTTGT
    GATGTTAATG TAGTAGCTGC TTCAGCGGTG TCGGTGTCCA AATCTGGCCC CACTCCCAAT
    CCCACATCCA CTCCAAATAT GGTATCCTCC TCTCCCAATT GTAACTTGCG TAGAAAGCCC
    TCCAATGAAA TGGTCCATCA TCGCAATTTG CTTAAAATGT TCAAGGAAAC CTTTCAAAAC
    GACGACGATC TGAAAGAGGT CTGCGAGATA GTCACTTCGC CTGGTCAGTT TACCCAGATC
    ATTGACTTTT CCAAGCGGCC AAGTAATCCC CCCATACCAA TACCTACTCT ACTACCTACC
    TCTACTCCGA TTCCTACCCA GATACCGCGA CCACTGGGCA AGCCGCCCAT GTCGCCCAAG
    GCTGTGAGGT TAGCGTCCAG TCCTAAGGGA CATATAAATC CTTTAAGTTC GCCCACGAAC
    TCCGGGTTTT CACCCCCCCA ATTCAGTATC AATGGGCGTG TGGAGATTTT TAATTCACGG
    CAACTGGACA AGCCGCCCAC GCCACCAGCT CCTCCCAAGA AAACTGTAAG GAGTGAGCAT
    CCTCTTAAGG AAGCGGTTCC TTCACCAGAA GTGGTGATGT GCGCTCCTCC TATGACCATC
    GTTGATCCCG ACGAGCTGCC CAAACCAAAT ACTGTAAAGG CCCTTTCAAA TTGCTTCAGC
    CAGAGCGATT CCAAGTCGCT AACATCACCC ATAATGTCGC CGAAACCTTT GCCCAGTGGT
    CGCATTCCCC AGATCACACC ACCTGCTTCT CCAAAGCCCC CGAAACGAGT TCAGCTTGAC
    GAGGTGGATC GTAGCTCTGG GGACTATGCC CCAGTTGTTG GTCAAACCAA GGCAATTGTA
    CACACGCCCC CTAGCAAAAT AAATGCCCGG GCAAACCAAA CTCTTGATCC CCTAGCACCC
    AACGACTCAC TGTGCCTGCA CACATCTTCG CCCAGCGAGA TACCTTCCTT GAAAGCCTGC
    TCCCCGAATG AATCCAGTTT GTTCAAGCTG GCTTCGTCAC GACCCACCTC GCCTTCAGTG
    GATGTGGCTT CGTGTCAGAA ATTTTTGAAT AGAGAAGCAC CAAAGGATAT CCAACTGGCC
    GAAGAAGACC GCAAGAGAAT GGATCAGTTT GCTGTGGAGG AGATGCCCTG TGATAAGATC
    GATGTGACCA AATCACCCCA GCCCAATACT GGTTTCCAGA TTGTGACCGA GGATCTAGTA
    AAAAAATGTG ATGGCAAAAA CATATCCGAA GCGGTAAGGG ATCTAGACGT CCCACCCAGC
    CAAGAAGATA TTGCCCTGTG TGCGGGACTA CTAGACTGTT TGGCTCCAGC TCAGGAGCCT
    ATTTGTGACA GCATCGATGT GAGGAATGGT CCAGTGCCGG CAACTGGATT TCAGGACATC
    AGTGAGGAGG AGGTGAAAAA GTGTGAAAAG CATAATAAAA ATTATGTTAT CACAAACAGG
    CCAAGCTCAC CCATTGGCAG TGTCAGAGCA GCTCACTCGA AGGCTTTGAT GGACACTTGT
    GAGCAGATCA TTGCAGAGGA GCGCAAAGCT AGCAGTCAGA TGATGAAGAC TTCGCTGCCG
    GAGTCGGTGG AGAAACCGTC GACACCCCTC CCAGGTCGCA AATCCAAGAT TCCCATACCC
    AAGCCTTGCT GTATTTCGGC ATCTGGAACT GATTCAATAA GTAGAGCGGG CTATGTACCA
    ACTGCACCGT CACACCTCTC AGGAAGTGCC CAGCAGCCGT TAATAGCACG CACCCATGTG
    GTGGAAACAG AGATCAAGGT CAAGGAGATC TCCCCACCCA GTATACCTAA GACACCGGAA
    CAAACTCAGC TCCATGCATC ACCAACTAAG GAGAAGAAAG CCAAGAACAT ATTTGATTTC
    CTGCGCAGAA ACTTTGGGCA TCAGGAAGAG CCGCAGCATC ATTCCACTCT GAATGAGACC
    CTTGAGAAAA AGGTGGTGCT CACCAGCACC AAGAGTGGAG TAGATGTGGT CAATGCCGAT
    GAGTTTGTTC GGGTAGACAA TTCAAAGTTC TATTTGGCAA GTGAGCTGGA GGACGTACCA
    GTGCCACCTC CTCTGCCCAA GACACCTGCT CCTGTCAACA TTGAGATTCG AAAAAAGATC
    ACTACAAATG AGATATTAGA GGAAAATACA ACAGAACAGG CGTTAACCCA GGAAATAAGC
    GATTTGCTAG ACGACGAGAT CATGAAACTG GAGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_730864.1
    CDS614..4732
    Translation

    Target ORF information:

    RefSeq Version NM_169014.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster smallish (smash), transcript variant E, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_169014.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGGCCA CTGATCAGGA GGTCATGCTG GAGGGCAGGG AAGACCTCAT GTCCACATCG 
    GCGACATCGT CCACAAATGG CGGGGACACT AATGGTTGCG GCAAAAAATT GTCCGTAACC
    TCTCCGGATC CCACACCGTA CGTGACCAAG CAGCGAGTGA CGCGTCCAAC GCCTCCCGCT
    CCTTCAAAGA ATCCGATGCA ATTCGTCCAG ATCAAGCCCT GCAACCTCTA CCAGACCGCG
    CAGGAGCAGC TGAAGAAGGC CGAGGAGGTC AAGAAGCTGA AGGAGGTGAA GAAGGAGGAG
    CCAGAGGAGT GGCAGAACAA CCTTGACAAT TGGAAATCAT CGAGACGAAA ACGTGTCGAA
    CACATCATCG ATCGAGCGGT GGAGACCAAG AAACTCGAGC TGGAGGAGCA TGATCGGTTG
    CGCCGAAAGT CGAAAACATT CACCGAAATG ATGGAGGAGA GGGCTGAAAG GGGCGGACCT
    CGGGGGCGTG CCAAGCTGGC TTCCCTGGCC GTATACAACG AGGATGAGAC GAACGACCTG
    AGTGATTTGG GGATCGGAAC CAGCAGTGCC AGCGGAAAGA GCAGCCTGTC CGGGGACTAT
    GACAATAACA GTGTTATGAG CGACCATGCG GCGGAGCTGG ATAAGGCGAT TGGAGCAGCT
    AGCTCTGTAG CTGCTGGAGT AACCGCCCAA AACGTGGATG AGCAGCTGAA TCACATAAAC
    CGCAACGGTA GTAATGGGAA CCACGGGAAC GGAGCGGCTG TTGGCCAGAC AGGACCATCA
    AATTCCTCCA AGTCAGCAGG GCGGCAATAC ATCTCCTCGC CAGGATACGA TACGTCCTCC
    AGTAACGCGC AAGCCAGTTC TCCGGATCTC TGTGAATACA CCTACGAAGG AGCCATCCAG
    GACTACAAGC AAAGAGTTCA ACGGGCCAGC AGCAATGGCA ATGGAAATGC CAACGGCAAG
    TTGATTGGAG AACATATTGC TTATCCCACG AGGCGAGGCT CTAAAATTGA GGATCGATTG
    AGTGGTTTTG AGGTGACATC GCCAAGCGAC ACGCAGGAGG GTGTAGAGAA GCAAAAGGTG
    GACGTGCCCA AGGTTGACAT ATCCAAACGC AAGGAGATCT TCGAGCAGGC CAAGGCGGAA
    GTCTCAAACG GTGGTGCTCC AGCTGCCCCA AAACTCGTAT TTCGTGATGG TCTTACCAAT
    GGAAATTCAG CTACCACTGC TCCCAAATCG GAGGTGAGGC GTCTTTCTGG TGACATCTCC
    AGCATTCGAG ATCGCATGCA GAGTTTGGAA CAGCAGCGGA AGGCTTTCAG TTCCAGCAAG
    AGTGTGGATG TGCCGGTGCC GCCCCTTAAA CAGCGTCTGA ATAGTCTGCA GCAGTCCGTT
    GCCAAAGAGG AGCAGAAGAA GCCACCGTTG GTGGCGCTCA TCGATGCCAG ACAACTGGAG
    ATCATGAGGG GAGAAGAGGA ACGTATGCGC CAGCAGCAAC AAAAAGAGAA GCATTCACCA
    AGCCAAACTA CATTGTGTGC ACCTAAGCCA GCTCCTCCTG CCCTTATTAT TGAGGAACCG
    CCGGTGGCAG ACACCAACGA CGACAGCGGC ATTCAGGAGG ACACCGCTGA TGAACTGCAG
    CAGCAGCAGC AACTCAACGC AGCCATCGCC GCCTTGGCGC TCGAGGAGCG CCAGTTGGAG
    GAAGCAGCCA ATGCGGTCAA CCAAATTGAA GCGGAGTTCG ATGAGCTGAC TGATCTGCAT
    CCCTCGCCAT TGCCACCATC TCCAGCTCTT CCGGCGGTTC AACAAAGCCT CCAACAACCG
    ACAGTCGCTC AGGTTCCAGC ACAAGCGGTC AATCAGGCGG CTGCTCCTCC ATTGCGGGAC
    ATGGAATTTA GTGTAAGTTG TACTAACAAT TTTATCGAAC CCGCTAAGAT AAGTCCTTGT
    GATGTTAATG TAGTAGCTGC TTCAGCGGTG TCGGTGTCCA AATCTGGCCC CACTCCCAAT
    CCCACATCCA CTCCAAATAT GGTATCCTCC TCTCCCAATT GTAACTTGCG TAGAAAGCCC
    TCCAATGAAA TGGTCCATCA TCGCAATTTG CTTAAAATGT TCAAGGAAAC CTTTCAAAAC
    GACGACGATC TGAAAGAGGT CTGCGAGATA GTCACTTCGC CTGGTCAGTT TACCCAGATC
    ATTGACTTTT CCAAGCGGCC AAGTAATCCC CCCATACCAA TACCTACTCT ACTACCTACC
    TCTACTCCGA TTCCTACCCA GATACCGCGA CCACTGGGCA AGCCGCCCAT GTCGCCCAAG
    GCTGTGAGGT TAGCGTCCAG TCCTAAGGGA CATATAAATC CTTTAAGTTC GCCCACGAAC
    TCCGGGTTTT CACCCCCCCA ATTCAGTATC AATGGGCGTG TGGAGATTTT TAATTCACGG
    CAACTGGACA AGCCGCCCAC GCCACCAGCT CCTCCCAAGA AAACTGTAAG GAGTGAGCAT
    CCTCTTAAGG AAGCGGTTCC TTCACCAGAA GTGGTGATGT GCGCTCCTCC TATGACCATC
    GTTGATCCCG ACGAGCTGCC CAAACCAAAT ACTGTAAAGG CCCTTTCAAA TTGCTTCAGC
    CAGAGCGATT CCAAGTCGCT AACATCACCC ATAATGTCGC CGAAACCTTT GCCCAGTGGT
    CGCATTCCCC AGATCACACC ACCTGCTTCT CCAAAGCCCC CGAAACGAGT TCAGCTTGAC
    GAGGTGGATC GTAGCTCTGG GGACTATGCC CCAGTTGTTG GTCAAACCAA GGCAATTGTA
    CACACGCCCC CTAGCAAAAT AAATGCCCGG GCAAACCAAA CTCTTGATCC CCTAGCACCC
    AACGACTCAC TGTGCCTGCA CACATCTTCG CCCAGCGAGA TACCTTCCTT GAAAGCCTGC
    TCCCCGAATG AATCCAGTTT GTTCAAGCTG GCTTCGTCAC GACCCACCTC GCCTTCAGTG
    GATGTGGCTT CGTGTCAGAA ATTTTTGAAT AGAGAAGCAC CAAAGGATAT CCAACTGGCC
    GAAGAAGACC GCAAGAGAAT GGATCAGTTT GCTGTGGAGG AGATGCCCTG TGATAAGATC
    GATGTGACCA AATCACCCCA GCCCAATACT GGTTTCCAGA TTGTGACCGA GGATCTAGTA
    AAAAAATGTG ATGGCAAAAA CATATCCGAA GCGGTAAGGG ATCTAGACGT CCCACCCAGC
    CAAGAAGATA TTGCCCTGTG TGCGGGACTA CTAGACTGTT TGGCTCCAGC TCAGGAGCCT
    ATTTGTGACA GCATCGATGT GAGGAATGGT CCAGTGCCGG CAACTGGATT TCAGGACATC
    AGTGAGGAGG AGGTGAAAAA GTGTGAAAAG CATAATAAAA ATTATGTTAT CACAAACAGG
    CCAAGCTCAC CCATTGGCAG TGTCAGAGCA GCTCACTCGA AGGCTTTGAT GGACACTTGT
    GAGCAGATCA TTGCAGAGGA GCGCAAAGCT AGCAGTCAGA TGATGAAGAC TTCGCTGCCG
    GAGTCGGTGG AGAAACCGTC GACACCCCTC CCAGGTCGCA AATCCAAGAT TCCCATACCC
    AAGCCTTGCT GTATTTCGGC ATCTGGAACT GATTCAATAA GTAGAGCGGG CTATGTACCA
    ACTGCACCGT CACACCTCTC AGGAAGTGCC CAGCAGCCGT TAATAGCACG CACCCATGTG
    GTGGAAACAG AGATCAAGGT CAAGGAGATC TCCCCACCCA GTATACCTAA GACACCGGAA
    CAAACTCAGC TCCATGCATC ACCAACTAAG GAGAAGAAAG CCAAGAACAT ATTTGATTTC
    CTGCGCAGAA ACTTTGGGCA TCAGGAAGAG CCGCAGCATC ATTCCACTCT GAATGAGACC
    CTTGAGAAAA AGGTGGTGCT CACCAGCACC AAGAGTGGAG TAGATGTGGT CAATGCCGAT
    GAGTTTGTTC GGGTAGACAA TTCAAAGTTC TATTTGGCAA GTGAGCTGGA GGACGTACCA
    GTGCCACCTC CTCTGCCCAA GACACCTGCT CCTGTCAACA TTGAGATTCG AAAAAAGATC
    ACTACAAATG AGATATTAGA GGAAAATACA ACAGAACAGG CGTTAACCCA GGAAATAAGC
    GATTTGCTAG ACGACGAGAT CATGAAACTG GAGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa03849
    Clone ID Related Accession (Same CDS sequence) NM_169014.3 , NM_176399.3 , NM_169015.3
    Accession Version NM_176399.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4119bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product smallish, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_176399.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGGCCA CTGATCAGGA GGTCATGCTG GAGGGCAGGG AAGACCTCAT GTCCACATCG 
    GCGACATCGT CCACAAATGG CGGGGACACT AATGGTTGCG GCAAAAAATT GTCCGTAACC
    TCTCCGGATC CCACACCGTA CGTGACCAAG CAGCGAGTGA CGCGTCCAAC GCCTCCCGCT
    CCTTCAAAGA ATCCGATGCA ATTCGTCCAG ATCAAGCCCT GCAACCTCTA CCAGACCGCG
    CAGGAGCAGC TGAAGAAGGC CGAGGAGGTC AAGAAGCTGA AGGAGGTGAA GAAGGAGGAG
    CCAGAGGAGT GGCAGAACAA CCTTGACAAT TGGAAATCAT CGAGACGAAA ACGTGTCGAA
    CACATCATCG ATCGAGCGGT GGAGACCAAG AAACTCGAGC TGGAGGAGCA TGATCGGTTG
    CGCCGAAAGT CGAAAACATT CACCGAAATG ATGGAGGAGA GGGCTGAAAG GGGCGGACCT
    CGGGGGCGTG CCAAGCTGGC TTCCCTGGCC GTATACAACG AGGATGAGAC GAACGACCTG
    AGTGATTTGG GGATCGGAAC CAGCAGTGCC AGCGGAAAGA GCAGCCTGTC CGGGGACTAT
    GACAATAACA GTGTTATGAG CGACCATGCG GCGGAGCTGG ATAAGGCGAT TGGAGCAGCT
    AGCTCTGTAG CTGCTGGAGT AACCGCCCAA AACGTGGATG AGCAGCTGAA TCACATAAAC
    CGCAACGGTA GTAATGGGAA CCACGGGAAC GGAGCGGCTG TTGGCCAGAC AGGACCATCA
    AATTCCTCCA AGTCAGCAGG GCGGCAATAC ATCTCCTCGC CAGGATACGA TACGTCCTCC
    AGTAACGCGC AAGCCAGTTC TCCGGATCTC TGTGAATACA CCTACGAAGG AGCCATCCAG
    GACTACAAGC AAAGAGTTCA ACGGGCCAGC AGCAATGGCA ATGGAAATGC CAACGGCAAG
    TTGATTGGAG AACATATTGC TTATCCCACG AGGCGAGGCT CTAAAATTGA GGATCGATTG
    AGTGGTTTTG AGGTGACATC GCCAAGCGAC ACGCAGGAGG GTGTAGAGAA GCAAAAGGTG
    GACGTGCCCA AGGTTGACAT ATCCAAACGC AAGGAGATCT TCGAGCAGGC CAAGGCGGAA
    GTCTCAAACG GTGGTGCTCC AGCTGCCCCA AAACTCGTAT TTCGTGATGG TCTTACCAAT
    GGAAATTCAG CTACCACTGC TCCCAAATCG GAGGTGAGGC GTCTTTCTGG TGACATCTCC
    AGCATTCGAG ATCGCATGCA GAGTTTGGAA CAGCAGCGGA AGGCTTTCAG TTCCAGCAAG
    AGTGTGGATG TGCCGGTGCC GCCCCTTAAA CAGCGTCTGA ATAGTCTGCA GCAGTCCGTT
    GCCAAAGAGG AGCAGAAGAA GCCACCGTTG GTGGCGCTCA TCGATGCCAG ACAACTGGAG
    ATCATGAGGG GAGAAGAGGA ACGTATGCGC CAGCAGCAAC AAAAAGAGAA GCATTCACCA
    AGCCAAACTA CATTGTGTGC ACCTAAGCCA GCTCCTCCTG CCCTTATTAT TGAGGAACCG
    CCGGTGGCAG ACACCAACGA CGACAGCGGC ATTCAGGAGG ACACCGCTGA TGAACTGCAG
    CAGCAGCAGC AACTCAACGC AGCCATCGCC GCCTTGGCGC TCGAGGAGCG CCAGTTGGAG
    GAAGCAGCCA ATGCGGTCAA CCAAATTGAA GCGGAGTTCG ATGAGCTGAC TGATCTGCAT
    CCCTCGCCAT TGCCACCATC TCCAGCTCTT CCGGCGGTTC AACAAAGCCT CCAACAACCG
    ACAGTCGCTC AGGTTCCAGC ACAAGCGGTC AATCAGGCGG CTGCTCCTCC ATTGCGGGAC
    ATGGAATTTA GTGTAAGTTG TACTAACAAT TTTATCGAAC CCGCTAAGAT AAGTCCTTGT
    GATGTTAATG TAGTAGCTGC TTCAGCGGTG TCGGTGTCCA AATCTGGCCC CACTCCCAAT
    CCCACATCCA CTCCAAATAT GGTATCCTCC TCTCCCAATT GTAACTTGCG TAGAAAGCCC
    TCCAATGAAA TGGTCCATCA TCGCAATTTG CTTAAAATGT TCAAGGAAAC CTTTCAAAAC
    GACGACGATC TGAAAGAGGT CTGCGAGATA GTCACTTCGC CTGGTCAGTT TACCCAGATC
    ATTGACTTTT CCAAGCGGCC AAGTAATCCC CCCATACCAA TACCTACTCT ACTACCTACC
    TCTACTCCGA TTCCTACCCA GATACCGCGA CCACTGGGCA AGCCGCCCAT GTCGCCCAAG
    GCTGTGAGGT TAGCGTCCAG TCCTAAGGGA CATATAAATC CTTTAAGTTC GCCCACGAAC
    TCCGGGTTTT CACCCCCCCA ATTCAGTATC AATGGGCGTG TGGAGATTTT TAATTCACGG
    CAACTGGACA AGCCGCCCAC GCCACCAGCT CCTCCCAAGA AAACTGTAAG GAGTGAGCAT
    CCTCTTAAGG AAGCGGTTCC TTCACCAGAA GTGGTGATGT GCGCTCCTCC TATGACCATC
    GTTGATCCCG ACGAGCTGCC CAAACCAAAT ACTGTAAAGG CCCTTTCAAA TTGCTTCAGC
    CAGAGCGATT CCAAGTCGCT AACATCACCC ATAATGTCGC CGAAACCTTT GCCCAGTGGT
    CGCATTCCCC AGATCACACC ACCTGCTTCT CCAAAGCCCC CGAAACGAGT TCAGCTTGAC
    GAGGTGGATC GTAGCTCTGG GGACTATGCC CCAGTTGTTG GTCAAACCAA GGCAATTGTA
    CACACGCCCC CTAGCAAAAT AAATGCCCGG GCAAACCAAA CTCTTGATCC CCTAGCACCC
    AACGACTCAC TGTGCCTGCA CACATCTTCG CCCAGCGAGA TACCTTCCTT GAAAGCCTGC
    TCCCCGAATG AATCCAGTTT GTTCAAGCTG GCTTCGTCAC GACCCACCTC GCCTTCAGTG
    GATGTGGCTT CGTGTCAGAA ATTTTTGAAT AGAGAAGCAC CAAAGGATAT CCAACTGGCC
    GAAGAAGACC GCAAGAGAAT GGATCAGTTT GCTGTGGAGG AGATGCCCTG TGATAAGATC
    GATGTGACCA AATCACCCCA GCCCAATACT GGTTTCCAGA TTGTGACCGA GGATCTAGTA
    AAAAAATGTG ATGGCAAAAA CATATCCGAA GCGGTAAGGG ATCTAGACGT CCCACCCAGC
    CAAGAAGATA TTGCCCTGTG TGCGGGACTA CTAGACTGTT TGGCTCCAGC TCAGGAGCCT
    ATTTGTGACA GCATCGATGT GAGGAATGGT CCAGTGCCGG CAACTGGATT TCAGGACATC
    AGTGAGGAGG AGGTGAAAAA GTGTGAAAAG CATAATAAAA ATTATGTTAT CACAAACAGG
    CCAAGCTCAC CCATTGGCAG TGTCAGAGCA GCTCACTCGA AGGCTTTGAT GGACACTTGT
    GAGCAGATCA TTGCAGAGGA GCGCAAAGCT AGCAGTCAGA TGATGAAGAC TTCGCTGCCG
    GAGTCGGTGG AGAAACCGTC GACACCCCTC CCAGGTCGCA AATCCAAGAT TCCCATACCC
    AAGCCTTGCT GTATTTCGGC ATCTGGAACT GATTCAATAA GTAGAGCGGG CTATGTACCA
    ACTGCACCGT CACACCTCTC AGGAAGTGCC CAGCAGCCGT TAATAGCACG CACCCATGTG
    GTGGAAACAG AGATCAAGGT CAAGGAGATC TCCCCACCCA GTATACCTAA GACACCGGAA
    CAAACTCAGC TCCATGCATC ACCAACTAAG GAGAAGAAAG CCAAGAACAT ATTTGATTTC
    CTGCGCAGAA ACTTTGGGCA TCAGGAAGAG CCGCAGCATC ATTCCACTCT GAATGAGACC
    CTTGAGAAAA AGGTGGTGCT CACCAGCACC AAGAGTGGAG TAGATGTGGT CAATGCCGAT
    GAGTTTGTTC GGGTAGACAA TTCAAAGTTC TATTTGGCAA GTGAGCTGGA GGACGTACCA
    GTGCCACCTC CTCTGCCCAA GACACCTGCT CCTGTCAACA TTGAGATTCG AAAAAAGATC
    ACTACAAATG AGATATTAGA GGAAAATACA ACAGAACAGG CGTTAACCCA GGAAATAAGC
    GATTTGCTAG ACGACGAGAT CATGAAACTG GAGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_788576.1
    CDS831..4949
    Translation

    Target ORF information:

    RefSeq Version NM_176399.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster smallish (smash), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_176399.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGGCCA CTGATCAGGA GGTCATGCTG GAGGGCAGGG AAGACCTCAT GTCCACATCG 
    GCGACATCGT CCACAAATGG CGGGGACACT AATGGTTGCG GCAAAAAATT GTCCGTAACC
    TCTCCGGATC CCACACCGTA CGTGACCAAG CAGCGAGTGA CGCGTCCAAC GCCTCCCGCT
    CCTTCAAAGA ATCCGATGCA ATTCGTCCAG ATCAAGCCCT GCAACCTCTA CCAGACCGCG
    CAGGAGCAGC TGAAGAAGGC CGAGGAGGTC AAGAAGCTGA AGGAGGTGAA GAAGGAGGAG
    CCAGAGGAGT GGCAGAACAA CCTTGACAAT TGGAAATCAT CGAGACGAAA ACGTGTCGAA
    CACATCATCG ATCGAGCGGT GGAGACCAAG AAACTCGAGC TGGAGGAGCA TGATCGGTTG
    CGCCGAAAGT CGAAAACATT CACCGAAATG ATGGAGGAGA GGGCTGAAAG GGGCGGACCT
    CGGGGGCGTG CCAAGCTGGC TTCCCTGGCC GTATACAACG AGGATGAGAC GAACGACCTG
    AGTGATTTGG GGATCGGAAC CAGCAGTGCC AGCGGAAAGA GCAGCCTGTC CGGGGACTAT
    GACAATAACA GTGTTATGAG CGACCATGCG GCGGAGCTGG ATAAGGCGAT TGGAGCAGCT
    AGCTCTGTAG CTGCTGGAGT AACCGCCCAA AACGTGGATG AGCAGCTGAA TCACATAAAC
    CGCAACGGTA GTAATGGGAA CCACGGGAAC GGAGCGGCTG TTGGCCAGAC AGGACCATCA
    AATTCCTCCA AGTCAGCAGG GCGGCAATAC ATCTCCTCGC CAGGATACGA TACGTCCTCC
    AGTAACGCGC AAGCCAGTTC TCCGGATCTC TGTGAATACA CCTACGAAGG AGCCATCCAG
    GACTACAAGC AAAGAGTTCA ACGGGCCAGC AGCAATGGCA ATGGAAATGC CAACGGCAAG
    TTGATTGGAG AACATATTGC TTATCCCACG AGGCGAGGCT CTAAAATTGA GGATCGATTG
    AGTGGTTTTG AGGTGACATC GCCAAGCGAC ACGCAGGAGG GTGTAGAGAA GCAAAAGGTG
    GACGTGCCCA AGGTTGACAT ATCCAAACGC AAGGAGATCT TCGAGCAGGC CAAGGCGGAA
    GTCTCAAACG GTGGTGCTCC AGCTGCCCCA AAACTCGTAT TTCGTGATGG TCTTACCAAT
    GGAAATTCAG CTACCACTGC TCCCAAATCG GAGGTGAGGC GTCTTTCTGG TGACATCTCC
    AGCATTCGAG ATCGCATGCA GAGTTTGGAA CAGCAGCGGA AGGCTTTCAG TTCCAGCAAG
    AGTGTGGATG TGCCGGTGCC GCCCCTTAAA CAGCGTCTGA ATAGTCTGCA GCAGTCCGTT
    GCCAAAGAGG AGCAGAAGAA GCCACCGTTG GTGGCGCTCA TCGATGCCAG ACAACTGGAG
    ATCATGAGGG GAGAAGAGGA ACGTATGCGC CAGCAGCAAC AAAAAGAGAA GCATTCACCA
    AGCCAAACTA CATTGTGTGC ACCTAAGCCA GCTCCTCCTG CCCTTATTAT TGAGGAACCG
    CCGGTGGCAG ACACCAACGA CGACAGCGGC ATTCAGGAGG ACACCGCTGA TGAACTGCAG
    CAGCAGCAGC AACTCAACGC AGCCATCGCC GCCTTGGCGC TCGAGGAGCG CCAGTTGGAG
    GAAGCAGCCA ATGCGGTCAA CCAAATTGAA GCGGAGTTCG ATGAGCTGAC TGATCTGCAT
    CCCTCGCCAT TGCCACCATC TCCAGCTCTT CCGGCGGTTC AACAAAGCCT CCAACAACCG
    ACAGTCGCTC AGGTTCCAGC ACAAGCGGTC AATCAGGCGG CTGCTCCTCC ATTGCGGGAC
    ATGGAATTTA GTGTAAGTTG TACTAACAAT TTTATCGAAC CCGCTAAGAT AAGTCCTTGT
    GATGTTAATG TAGTAGCTGC TTCAGCGGTG TCGGTGTCCA AATCTGGCCC CACTCCCAAT
    CCCACATCCA CTCCAAATAT GGTATCCTCC TCTCCCAATT GTAACTTGCG TAGAAAGCCC
    TCCAATGAAA TGGTCCATCA TCGCAATTTG CTTAAAATGT TCAAGGAAAC CTTTCAAAAC
    GACGACGATC TGAAAGAGGT CTGCGAGATA GTCACTTCGC CTGGTCAGTT TACCCAGATC
    ATTGACTTTT CCAAGCGGCC AAGTAATCCC CCCATACCAA TACCTACTCT ACTACCTACC
    TCTACTCCGA TTCCTACCCA GATACCGCGA CCACTGGGCA AGCCGCCCAT GTCGCCCAAG
    GCTGTGAGGT TAGCGTCCAG TCCTAAGGGA CATATAAATC CTTTAAGTTC GCCCACGAAC
    TCCGGGTTTT CACCCCCCCA ATTCAGTATC AATGGGCGTG TGGAGATTTT TAATTCACGG
    CAACTGGACA AGCCGCCCAC GCCACCAGCT CCTCCCAAGA AAACTGTAAG GAGTGAGCAT
    CCTCTTAAGG AAGCGGTTCC TTCACCAGAA GTGGTGATGT GCGCTCCTCC TATGACCATC
    GTTGATCCCG ACGAGCTGCC CAAACCAAAT ACTGTAAAGG CCCTTTCAAA TTGCTTCAGC
    CAGAGCGATT CCAAGTCGCT AACATCACCC ATAATGTCGC CGAAACCTTT GCCCAGTGGT
    CGCATTCCCC AGATCACACC ACCTGCTTCT CCAAAGCCCC CGAAACGAGT TCAGCTTGAC
    GAGGTGGATC GTAGCTCTGG GGACTATGCC CCAGTTGTTG GTCAAACCAA GGCAATTGTA
    CACACGCCCC CTAGCAAAAT AAATGCCCGG GCAAACCAAA CTCTTGATCC CCTAGCACCC
    AACGACTCAC TGTGCCTGCA CACATCTTCG CCCAGCGAGA TACCTTCCTT GAAAGCCTGC
    TCCCCGAATG AATCCAGTTT GTTCAAGCTG GCTTCGTCAC GACCCACCTC GCCTTCAGTG
    GATGTGGCTT CGTGTCAGAA ATTTTTGAAT AGAGAAGCAC CAAAGGATAT CCAACTGGCC
    GAAGAAGACC GCAAGAGAAT GGATCAGTTT GCTGTGGAGG AGATGCCCTG TGATAAGATC
    GATGTGACCA AATCACCCCA GCCCAATACT GGTTTCCAGA TTGTGACCGA GGATCTAGTA
    AAAAAATGTG ATGGCAAAAA CATATCCGAA GCGGTAAGGG ATCTAGACGT CCCACCCAGC
    CAAGAAGATA TTGCCCTGTG TGCGGGACTA CTAGACTGTT TGGCTCCAGC TCAGGAGCCT
    ATTTGTGACA GCATCGATGT GAGGAATGGT CCAGTGCCGG CAACTGGATT TCAGGACATC
    AGTGAGGAGG AGGTGAAAAA GTGTGAAAAG CATAATAAAA ATTATGTTAT CACAAACAGG
    CCAAGCTCAC CCATTGGCAG TGTCAGAGCA GCTCACTCGA AGGCTTTGAT GGACACTTGT
    GAGCAGATCA TTGCAGAGGA GCGCAAAGCT AGCAGTCAGA TGATGAAGAC TTCGCTGCCG
    GAGTCGGTGG AGAAACCGTC GACACCCCTC CCAGGTCGCA AATCCAAGAT TCCCATACCC
    AAGCCTTGCT GTATTTCGGC ATCTGGAACT GATTCAATAA GTAGAGCGGG CTATGTACCA
    ACTGCACCGT CACACCTCTC AGGAAGTGCC CAGCAGCCGT TAATAGCACG CACCCATGTG
    GTGGAAACAG AGATCAAGGT CAAGGAGATC TCCCCACCCA GTATACCTAA GACACCGGAA
    CAAACTCAGC TCCATGCATC ACCAACTAAG GAGAAGAAAG CCAAGAACAT ATTTGATTTC
    CTGCGCAGAA ACTTTGGGCA TCAGGAAGAG CCGCAGCATC ATTCCACTCT GAATGAGACC
    CTTGAGAAAA AGGTGGTGCT CACCAGCACC AAGAGTGGAG TAGATGTGGT CAATGCCGAT
    GAGTTTGTTC GGGTAGACAA TTCAAAGTTC TATTTGGCAA GTGAGCTGGA GGACGTACCA
    GTGCCACCTC CTCTGCCCAA GACACCTGCT CCTGTCAACA TTGAGATTCG AAAAAAGATC
    ACTACAAATG AGATATTAGA GGAAAATACA ACAGAACAGG CGTTAACCCA GGAAATAAGC
    GATTTGCTAG ACGACGAGAT CATGAAACTG GAGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa03849
    Clone ID Related Accession (Same CDS sequence) NM_169014.3 , NM_176399.3 , NM_169015.3
    Accession Version NM_169015.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4119bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product smallish, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_169015.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGGCCA CTGATCAGGA GGTCATGCTG GAGGGCAGGG AAGACCTCAT GTCCACATCG 
    GCGACATCGT CCACAAATGG CGGGGACACT AATGGTTGCG GCAAAAAATT GTCCGTAACC
    TCTCCGGATC CCACACCGTA CGTGACCAAG CAGCGAGTGA CGCGTCCAAC GCCTCCCGCT
    CCTTCAAAGA ATCCGATGCA ATTCGTCCAG ATCAAGCCCT GCAACCTCTA CCAGACCGCG
    CAGGAGCAGC TGAAGAAGGC CGAGGAGGTC AAGAAGCTGA AGGAGGTGAA GAAGGAGGAG
    CCAGAGGAGT GGCAGAACAA CCTTGACAAT TGGAAATCAT CGAGACGAAA ACGTGTCGAA
    CACATCATCG ATCGAGCGGT GGAGACCAAG AAACTCGAGC TGGAGGAGCA TGATCGGTTG
    CGCCGAAAGT CGAAAACATT CACCGAAATG ATGGAGGAGA GGGCTGAAAG GGGCGGACCT
    CGGGGGCGTG CCAAGCTGGC TTCCCTGGCC GTATACAACG AGGATGAGAC GAACGACCTG
    AGTGATTTGG GGATCGGAAC CAGCAGTGCC AGCGGAAAGA GCAGCCTGTC CGGGGACTAT
    GACAATAACA GTGTTATGAG CGACCATGCG GCGGAGCTGG ATAAGGCGAT TGGAGCAGCT
    AGCTCTGTAG CTGCTGGAGT AACCGCCCAA AACGTGGATG AGCAGCTGAA TCACATAAAC
    CGCAACGGTA GTAATGGGAA CCACGGGAAC GGAGCGGCTG TTGGCCAGAC AGGACCATCA
    AATTCCTCCA AGTCAGCAGG GCGGCAATAC ATCTCCTCGC CAGGATACGA TACGTCCTCC
    AGTAACGCGC AAGCCAGTTC TCCGGATCTC TGTGAATACA CCTACGAAGG AGCCATCCAG
    GACTACAAGC AAAGAGTTCA ACGGGCCAGC AGCAATGGCA ATGGAAATGC CAACGGCAAG
    TTGATTGGAG AACATATTGC TTATCCCACG AGGCGAGGCT CTAAAATTGA GGATCGATTG
    AGTGGTTTTG AGGTGACATC GCCAAGCGAC ACGCAGGAGG GTGTAGAGAA GCAAAAGGTG
    GACGTGCCCA AGGTTGACAT ATCCAAACGC AAGGAGATCT TCGAGCAGGC CAAGGCGGAA
    GTCTCAAACG GTGGTGCTCC AGCTGCCCCA AAACTCGTAT TTCGTGATGG TCTTACCAAT
    GGAAATTCAG CTACCACTGC TCCCAAATCG GAGGTGAGGC GTCTTTCTGG TGACATCTCC
    AGCATTCGAG ATCGCATGCA GAGTTTGGAA CAGCAGCGGA AGGCTTTCAG TTCCAGCAAG
    AGTGTGGATG TGCCGGTGCC GCCCCTTAAA CAGCGTCTGA ATAGTCTGCA GCAGTCCGTT
    GCCAAAGAGG AGCAGAAGAA GCCACCGTTG GTGGCGCTCA TCGATGCCAG ACAACTGGAG
    ATCATGAGGG GAGAAGAGGA ACGTATGCGC CAGCAGCAAC AAAAAGAGAA GCATTCACCA
    AGCCAAACTA CATTGTGTGC ACCTAAGCCA GCTCCTCCTG CCCTTATTAT TGAGGAACCG
    CCGGTGGCAG ACACCAACGA CGACAGCGGC ATTCAGGAGG ACACCGCTGA TGAACTGCAG
    CAGCAGCAGC AACTCAACGC AGCCATCGCC GCCTTGGCGC TCGAGGAGCG CCAGTTGGAG
    GAAGCAGCCA ATGCGGTCAA CCAAATTGAA GCGGAGTTCG ATGAGCTGAC TGATCTGCAT
    CCCTCGCCAT TGCCACCATC TCCAGCTCTT CCGGCGGTTC AACAAAGCCT CCAACAACCG
    ACAGTCGCTC AGGTTCCAGC ACAAGCGGTC AATCAGGCGG CTGCTCCTCC ATTGCGGGAC
    ATGGAATTTA GTGTAAGTTG TACTAACAAT TTTATCGAAC CCGCTAAGAT AAGTCCTTGT
    GATGTTAATG TAGTAGCTGC TTCAGCGGTG TCGGTGTCCA AATCTGGCCC CACTCCCAAT
    CCCACATCCA CTCCAAATAT GGTATCCTCC TCTCCCAATT GTAACTTGCG TAGAAAGCCC
    TCCAATGAAA TGGTCCATCA TCGCAATTTG CTTAAAATGT TCAAGGAAAC CTTTCAAAAC
    GACGACGATC TGAAAGAGGT CTGCGAGATA GTCACTTCGC CTGGTCAGTT TACCCAGATC
    ATTGACTTTT CCAAGCGGCC AAGTAATCCC CCCATACCAA TACCTACTCT ACTACCTACC
    TCTACTCCGA TTCCTACCCA GATACCGCGA CCACTGGGCA AGCCGCCCAT GTCGCCCAAG
    GCTGTGAGGT TAGCGTCCAG TCCTAAGGGA CATATAAATC CTTTAAGTTC GCCCACGAAC
    TCCGGGTTTT CACCCCCCCA ATTCAGTATC AATGGGCGTG TGGAGATTTT TAATTCACGG
    CAACTGGACA AGCCGCCCAC GCCACCAGCT CCTCCCAAGA AAACTGTAAG GAGTGAGCAT
    CCTCTTAAGG AAGCGGTTCC TTCACCAGAA GTGGTGATGT GCGCTCCTCC TATGACCATC
    GTTGATCCCG ACGAGCTGCC CAAACCAAAT ACTGTAAAGG CCCTTTCAAA TTGCTTCAGC
    CAGAGCGATT CCAAGTCGCT AACATCACCC ATAATGTCGC CGAAACCTTT GCCCAGTGGT
    CGCATTCCCC AGATCACACC ACCTGCTTCT CCAAAGCCCC CGAAACGAGT TCAGCTTGAC
    GAGGTGGATC GTAGCTCTGG GGACTATGCC CCAGTTGTTG GTCAAACCAA GGCAATTGTA
    CACACGCCCC CTAGCAAAAT AAATGCCCGG GCAAACCAAA CTCTTGATCC CCTAGCACCC
    AACGACTCAC TGTGCCTGCA CACATCTTCG CCCAGCGAGA TACCTTCCTT GAAAGCCTGC
    TCCCCGAATG AATCCAGTTT GTTCAAGCTG GCTTCGTCAC GACCCACCTC GCCTTCAGTG
    GATGTGGCTT CGTGTCAGAA ATTTTTGAAT AGAGAAGCAC CAAAGGATAT CCAACTGGCC
    GAAGAAGACC GCAAGAGAAT GGATCAGTTT GCTGTGGAGG AGATGCCCTG TGATAAGATC
    GATGTGACCA AATCACCCCA GCCCAATACT GGTTTCCAGA TTGTGACCGA GGATCTAGTA
    AAAAAATGTG ATGGCAAAAA CATATCCGAA GCGGTAAGGG ATCTAGACGT CCCACCCAGC
    CAAGAAGATA TTGCCCTGTG TGCGGGACTA CTAGACTGTT TGGCTCCAGC TCAGGAGCCT
    ATTTGTGACA GCATCGATGT GAGGAATGGT CCAGTGCCGG CAACTGGATT TCAGGACATC
    AGTGAGGAGG AGGTGAAAAA GTGTGAAAAG CATAATAAAA ATTATGTTAT CACAAACAGG
    CCAAGCTCAC CCATTGGCAG TGTCAGAGCA GCTCACTCGA AGGCTTTGAT GGACACTTGT
    GAGCAGATCA TTGCAGAGGA GCGCAAAGCT AGCAGTCAGA TGATGAAGAC TTCGCTGCCG
    GAGTCGGTGG AGAAACCGTC GACACCCCTC CCAGGTCGCA AATCCAAGAT TCCCATACCC
    AAGCCTTGCT GTATTTCGGC ATCTGGAACT GATTCAATAA GTAGAGCGGG CTATGTACCA
    ACTGCACCGT CACACCTCTC AGGAAGTGCC CAGCAGCCGT TAATAGCACG CACCCATGTG
    GTGGAAACAG AGATCAAGGT CAAGGAGATC TCCCCACCCA GTATACCTAA GACACCGGAA
    CAAACTCAGC TCCATGCATC ACCAACTAAG GAGAAGAAAG CCAAGAACAT ATTTGATTTC
    CTGCGCAGAA ACTTTGGGCA TCAGGAAGAG CCGCAGCATC ATTCCACTCT GAATGAGACC
    CTTGAGAAAA AGGTGGTGCT CACCAGCACC AAGAGTGGAG TAGATGTGGT CAATGCCGAT
    GAGTTTGTTC GGGTAGACAA TTCAAAGTTC TATTTGGCAA GTGAGCTGGA GGACGTACCA
    GTGCCACCTC CTCTGCCCAA GACACCTGCT CCTGTCAACA TTGAGATTCG AAAAAAGATC
    ACTACAAATG AGATATTAGA GGAAAATACA ACAGAACAGG CGTTAACCCA GGAAATAAGC
    GATTTGCTAG ACGACGAGAT CATGAAACTG GAGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_730865.1
    CDS1176..5294
    Translation

    Target ORF information:

    RefSeq Version NM_169015.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster smallish (smash), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_169015.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGAGGCCA CTGATCAGGA GGTCATGCTG GAGGGCAGGG AAGACCTCAT GTCCACATCG 
    GCGACATCGT CCACAAATGG CGGGGACACT AATGGTTGCG GCAAAAAATT GTCCGTAACC
    TCTCCGGATC CCACACCGTA CGTGACCAAG CAGCGAGTGA CGCGTCCAAC GCCTCCCGCT
    CCTTCAAAGA ATCCGATGCA ATTCGTCCAG ATCAAGCCCT GCAACCTCTA CCAGACCGCG
    CAGGAGCAGC TGAAGAAGGC CGAGGAGGTC AAGAAGCTGA AGGAGGTGAA GAAGGAGGAG
    CCAGAGGAGT GGCAGAACAA CCTTGACAAT TGGAAATCAT CGAGACGAAA ACGTGTCGAA
    CACATCATCG ATCGAGCGGT GGAGACCAAG AAACTCGAGC TGGAGGAGCA TGATCGGTTG
    CGCCGAAAGT CGAAAACATT CACCGAAATG ATGGAGGAGA GGGCTGAAAG GGGCGGACCT
    CGGGGGCGTG CCAAGCTGGC TTCCCTGGCC GTATACAACG AGGATGAGAC GAACGACCTG
    AGTGATTTGG GGATCGGAAC CAGCAGTGCC AGCGGAAAGA GCAGCCTGTC CGGGGACTAT
    GACAATAACA GTGTTATGAG CGACCATGCG GCGGAGCTGG ATAAGGCGAT TGGAGCAGCT
    AGCTCTGTAG CTGCTGGAGT AACCGCCCAA AACGTGGATG AGCAGCTGAA TCACATAAAC
    CGCAACGGTA GTAATGGGAA CCACGGGAAC GGAGCGGCTG TTGGCCAGAC AGGACCATCA
    AATTCCTCCA AGTCAGCAGG GCGGCAATAC ATCTCCTCGC CAGGATACGA TACGTCCTCC
    AGTAACGCGC AAGCCAGTTC TCCGGATCTC TGTGAATACA CCTACGAAGG AGCCATCCAG
    GACTACAAGC AAAGAGTTCA ACGGGCCAGC AGCAATGGCA ATGGAAATGC CAACGGCAAG
    TTGATTGGAG AACATATTGC TTATCCCACG AGGCGAGGCT CTAAAATTGA GGATCGATTG
    AGTGGTTTTG AGGTGACATC GCCAAGCGAC ACGCAGGAGG GTGTAGAGAA GCAAAAGGTG
    GACGTGCCCA AGGTTGACAT ATCCAAACGC AAGGAGATCT TCGAGCAGGC CAAGGCGGAA
    GTCTCAAACG GTGGTGCTCC AGCTGCCCCA AAACTCGTAT TTCGTGATGG TCTTACCAAT
    GGAAATTCAG CTACCACTGC TCCCAAATCG GAGGTGAGGC GTCTTTCTGG TGACATCTCC
    AGCATTCGAG ATCGCATGCA GAGTTTGGAA CAGCAGCGGA AGGCTTTCAG TTCCAGCAAG
    AGTGTGGATG TGCCGGTGCC GCCCCTTAAA CAGCGTCTGA ATAGTCTGCA GCAGTCCGTT
    GCCAAAGAGG AGCAGAAGAA GCCACCGTTG GTGGCGCTCA TCGATGCCAG ACAACTGGAG
    ATCATGAGGG GAGAAGAGGA ACGTATGCGC CAGCAGCAAC AAAAAGAGAA GCATTCACCA
    AGCCAAACTA CATTGTGTGC ACCTAAGCCA GCTCCTCCTG CCCTTATTAT TGAGGAACCG
    CCGGTGGCAG ACACCAACGA CGACAGCGGC ATTCAGGAGG ACACCGCTGA TGAACTGCAG
    CAGCAGCAGC AACTCAACGC AGCCATCGCC GCCTTGGCGC TCGAGGAGCG CCAGTTGGAG
    GAAGCAGCCA ATGCGGTCAA CCAAATTGAA GCGGAGTTCG ATGAGCTGAC TGATCTGCAT
    CCCTCGCCAT TGCCACCATC TCCAGCTCTT CCGGCGGTTC AACAAAGCCT CCAACAACCG
    ACAGTCGCTC AGGTTCCAGC ACAAGCGGTC AATCAGGCGG CTGCTCCTCC ATTGCGGGAC
    ATGGAATTTA GTGTAAGTTG TACTAACAAT TTTATCGAAC CCGCTAAGAT AAGTCCTTGT
    GATGTTAATG TAGTAGCTGC TTCAGCGGTG TCGGTGTCCA AATCTGGCCC CACTCCCAAT
    CCCACATCCA CTCCAAATAT GGTATCCTCC TCTCCCAATT GTAACTTGCG TAGAAAGCCC
    TCCAATGAAA TGGTCCATCA TCGCAATTTG CTTAAAATGT TCAAGGAAAC CTTTCAAAAC
    GACGACGATC TGAAAGAGGT CTGCGAGATA GTCACTTCGC CTGGTCAGTT TACCCAGATC
    ATTGACTTTT CCAAGCGGCC AAGTAATCCC CCCATACCAA TACCTACTCT ACTACCTACC
    TCTACTCCGA TTCCTACCCA GATACCGCGA CCACTGGGCA AGCCGCCCAT GTCGCCCAAG
    GCTGTGAGGT TAGCGTCCAG TCCTAAGGGA CATATAAATC CTTTAAGTTC GCCCACGAAC
    TCCGGGTTTT CACCCCCCCA ATTCAGTATC AATGGGCGTG TGGAGATTTT TAATTCACGG
    CAACTGGACA AGCCGCCCAC GCCACCAGCT CCTCCCAAGA AAACTGTAAG GAGTGAGCAT
    CCTCTTAAGG AAGCGGTTCC TTCACCAGAA GTGGTGATGT GCGCTCCTCC TATGACCATC
    GTTGATCCCG ACGAGCTGCC CAAACCAAAT ACTGTAAAGG CCCTTTCAAA TTGCTTCAGC
    CAGAGCGATT CCAAGTCGCT AACATCACCC ATAATGTCGC CGAAACCTTT GCCCAGTGGT
    CGCATTCCCC AGATCACACC ACCTGCTTCT CCAAAGCCCC CGAAACGAGT TCAGCTTGAC
    GAGGTGGATC GTAGCTCTGG GGACTATGCC CCAGTTGTTG GTCAAACCAA GGCAATTGTA
    CACACGCCCC CTAGCAAAAT AAATGCCCGG GCAAACCAAA CTCTTGATCC CCTAGCACCC
    AACGACTCAC TGTGCCTGCA CACATCTTCG CCCAGCGAGA TACCTTCCTT GAAAGCCTGC
    TCCCCGAATG AATCCAGTTT GTTCAAGCTG GCTTCGTCAC GACCCACCTC GCCTTCAGTG
    GATGTGGCTT CGTGTCAGAA ATTTTTGAAT AGAGAAGCAC CAAAGGATAT CCAACTGGCC
    GAAGAAGACC GCAAGAGAAT GGATCAGTTT GCTGTGGAGG AGATGCCCTG TGATAAGATC
    GATGTGACCA AATCACCCCA GCCCAATACT GGTTTCCAGA TTGTGACCGA GGATCTAGTA
    AAAAAATGTG ATGGCAAAAA CATATCCGAA GCGGTAAGGG ATCTAGACGT CCCACCCAGC
    CAAGAAGATA TTGCCCTGTG TGCGGGACTA CTAGACTGTT TGGCTCCAGC TCAGGAGCCT
    ATTTGTGACA GCATCGATGT GAGGAATGGT CCAGTGCCGG CAACTGGATT TCAGGACATC
    AGTGAGGAGG AGGTGAAAAA GTGTGAAAAG CATAATAAAA ATTATGTTAT CACAAACAGG
    CCAAGCTCAC CCATTGGCAG TGTCAGAGCA GCTCACTCGA AGGCTTTGAT GGACACTTGT
    GAGCAGATCA TTGCAGAGGA GCGCAAAGCT AGCAGTCAGA TGATGAAGAC TTCGCTGCCG
    GAGTCGGTGG AGAAACCGTC GACACCCCTC CCAGGTCGCA AATCCAAGAT TCCCATACCC
    AAGCCTTGCT GTATTTCGGC ATCTGGAACT GATTCAATAA GTAGAGCGGG CTATGTACCA
    ACTGCACCGT CACACCTCTC AGGAAGTGCC CAGCAGCCGT TAATAGCACG CACCCATGTG
    GTGGAAACAG AGATCAAGGT CAAGGAGATC TCCCCACCCA GTATACCTAA GACACCGGAA
    CAAACTCAGC TCCATGCATC ACCAACTAAG GAGAAGAAAG CCAAGAACAT ATTTGATTTC
    CTGCGCAGAA ACTTTGGGCA TCAGGAAGAG CCGCAGCATC ATTCCACTCT GAATGAGACC
    CTTGAGAAAA AGGTGGTGCT CACCAGCACC AAGAGTGGAG TAGATGTGGT CAATGCCGAT
    GAGTTTGTTC GGGTAGACAA TTCAAAGTTC TATTTGGCAA GTGAGCTGGA GGACGTACCA
    GTGCCACCTC CTCTGCCCAA GACACCTGCT CCTGTCAACA TTGAGATTCG AAAAAAGATC
    ACTACAAATG AGATATTAGA GGAAAATACA ACAGAACAGG CGTTAACCCA GGAAATAAGC
    GATTTGCTAG ACGACGAGAT CATGAAACTG GAGAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08054
    Clone ID Related Accession (Same CDS sequence) NM_001259984.2
    Accession Version NM_001259984.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4506bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product smallish, isoform P
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001259984.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAGGCCA CTGATCAGGA GGTCATGCTG GAGGGCAGGG AAGACCTCAT GTCCACATCG 
    GCGACATCGT CCACAAATGG CGGGGACACT AATGGTTGCG GCAAAAAATT GTCCGTAACC
    TCTCCGGATC CCACACCGTA CGTGACCAAG CAGCGAGTGA CGCGTCCAAC GCCTCCCGCT
    CCTTCAAAGA ATCCGATGCA ATTCGTCCAG ATCAAGCCCT GCAACCTCTA CCAGACCGCG
    CAGGAGCAGC TGAAGAAGGC CGAGGAGGTC AAGAAGCTGA AGGAGGTGAA GAAGGAGGAG
    CCAGAGGAGT GGCAGAACAA CCTTGACAAT TGGAAATCAT CGAGACGAAA ACGTGTCGAA
    CACATCATCG ATCGAGCGGT GGAGACCAAG AAACTCGAGC TGGAGGAGCA TGATCGGTTG
    CGCCGAAAGT CGAAAACATT CACCGAAATG ATGGAGGAGA GGGCTGAAAG GGGCGGACCT
    CGGGGGCGTG CCAAGCTGGC TTCCCTGGCC GTATACAACG AGGATGAGAC GAACGACCTG
    AGTGATTTGG GGATCGGAAC CAGCAGTGCC AGCGGAAAGA GCAGCCTGTC CGGGGACTAT
    GACAATAACA GTGTTATGAG CGACCATGCG GCGGAGCTGG ATAAGGCGAT TGGAGCAGCT
    AGCTCTGTAG CTGCTGGAGT AACCGCCCAA AACGTGGATG AGCAGCTGAA TCACATAAAC
    CGCAACGGTA GTAATGGGAA CCACGGGAAC GGAGCGGCTG TTGGCCAGAC AGGACCATCA
    AATTCCTCCA AGTCAGCAGG GCGGCAATAC ATCTCCTCGC CAGGATACGA TACGTCCTCC
    AGTAACGCGC AAGCCAGTTC TCCGGATCTC TGTGAATACA CCTACGAAGG AGCCATCCAG
    GACTACAAGC AAAGAGTTCA ACGGGCCAGC AGCAATGGCA ATGGAAATGC CAACGGCAAG
    TTGATTGGAG AACATATTGC TTATCCCACG AGGCGAGGCT CTAAAATTGA GGATCGATTG
    AGTGGTTTTG AGGTGACATC GCCAAGCGAC ACGCAGGAGG GTGTAGAGAA GCAAAAGGTG
    GACGTGCCCA AGGTTGACAT ATCCAAACGC AAGGAGATCT TCGAGCAGGC CAAGGCGGAA
    GTCTCAAACG GTGGTGCTCC AGCTGCCCCA AAACTCGTAT TTCGTGATGG TCTTACCAAT
    GGAAATTCAG CTACCACTGC TCCCAAATCG GAGGTGAGGC GTCTTTCTGG TGACATCTCC
    AGCATTCGAG ATCGCATGCA GAGTTTGGAA CAGCAGCGGA AGGCTTTCAG TTCCAGCAAG
    AGTGTGGATG TGCCGGTGCC GCCCCTTAAA CAGCGTCTGA ATAGTCTGCA GCAGTCCGTT
    GCCAAAGAGG AGCAGAAGAA GCCACCGTTG GTGGCGCTCA TCGATGCCAG ACAACTGGAG
    ATCATGAGGG GAGAAGAGGA ACGTATGCGC CAGCAGCAAC AAAAAGAGAA GCATTCACCA
    AGCCAAACTA CATTGTGTGC ACCTAAGCCA GCTCCTCCTG CCCTTATTAT TGAGGAACCG
    CCGGTGGCAG ACACCAACGA CGACAGCGGC ATTCAGGAGG ACACCGCTGA TGAACTGCAG
    CAGCAGCAGC AACTCAACGC AGCCATCGCC GCCTTGGCGC TCGAGGAGCG CCAGTTGGAG
    GAAGCAGCCA ATGCGGTCAA CCAAATTGAA GCGGAGTTCG ATGAGCTGAC TGATCTGCAT
    CCCTCGCCAT TGCCACCATC TCCAGCTCTT CCGGCGGTTC AACAAAGCCT CCAACAACCG
    ACAGTCGCTC AGGTTCCAGC ACAAGCGGTC AATCAGGCGG CTGCTCCTCC ATTGCGGGAC
    ATGGAATTTA GTATCAACGA AGCTCTTGAA TTGGCGCTGC AAGCTATTGA TCGCGAGGCG
    ACAACCAAGC CAATGGACAA GGCACAACAT CCACAGAAGC GGGAACAGAA GGAGGAGCTG
    GGAGGTAAGC CGGAGGAGAA GGAGCCAATT TGCGAGGAGC AGCACGACGA AGAGTCCAAC
    GAGCAGGGGG AAACACACAA CGAGGGTGGG GAAATGCCAA ATAAAACCGA TAAGCAAATG
    CAGGGGGGAC GAGAGCCCAT CTATGAGAAT GTCAGCTCGA CTATATCTAT GCATGAAGTA
    GATAATGAAC AGATAGCAAC TATGACGATA ACCACACAGA CCCAAGCGAC GCGAATAAGT
    CACAGGAGAA GCATTCCAAG CCAGCAAACC AGCAACAGTA ACGAGAATAA TCAAAGCACT
    AATCAAACCA GCAACAGCAG GAATCAAAAA GAGAGCAGCA CAGCAGCAAC ATCGGCAGCG
    AACCCCGTGC CACCAACAAC ACCGGGGGAA CAAGCAGAGC CCTACTACCA AGTGCCGAAG
    GCCACGGAGC CCTACTACGA TGCCCCCAAG CATTTAAGGC CGGTGCCGGT ATACGAAAAT
    GTCGAGATCT TTTACTCGGG CCTGGAGATT AGTCAGGGAT CTGTGGGAGC GCCAGTAGGG
    CTGATGGAGC CACCCAAGGA AAAGCCACCA CCACCGCCCA CCGAGAGTCC GATTCCGCTG
    GACGAGGGCC ATGACGACGA GCTGGATGGA CTGGGCAACA GTCTGGGCCA CAACACAGAC
    ACCTGGTCAT CGGACAACAC CTACGAGACC ATATCGAACG GCACTCGCCG GCACCTTCAG
    GGACAACCGG AACCCGCCCA ACCCTCGCCG CCCATCAAGA GGATGAACTC GACCAAACGG
    ATCAAGAAGG AGTTGCGCAA CAAGCGCTCC AGCTTCCTGG GCATCGAAAA CGACGGCGAT
    CTTGACGACA TGGAGACCTA TCTCGAGCTA ACAGTGGCCC CGCCTCCGGA TATGGCGCAG
    CTCTTGCAGG AGGAGCGGCG ACTGGAGAAG CAGCTGTACA TCAAGGCGGG ACTCTGCGAC
    AGTTCCGACA CAGGTGAAAG CCGCGATTCT GGAGTTTCTG AAAACCACTC TCGCCAGAGC
    AGTGAGCACT ACACGAACTC CTCTGAGGAG AACGATACAC AGTCAGAGGC CACACCGCCG
    CCATTGCCTC CACCACCATC GACCGCCCAA GTGGGTGAAG TCATATACCA AAATGAGAGC
    CTCCTGGCTG CCCAGACGCC GCTTCTCCAG ACGGTAAAAG GAAACTCCGC CGAGTCCTGG
    ACCGAATCAG CAACGGCCGC AGCGGCGGCC ACGCAATCAT TGAGCGAAGC CACAGCAACG
    GCCAATGCCA AGATGCAGTC CATCGAGGAT AAGATCCGGG AGCAGGGAGA GATGTTGCGG
    GTGGAACGCG AGCTACTGCA CTTCTCGCAA GAGGAACTGA AACGGCAACG TGAAAATCTT
    GTGCTTCGAG AAAATATTGC TCGACGAGAG TTGCAACACG GAGCCAAGAT GCTGATGTCG
    AACAACCGGC GCTCACTGCA GGATCTGCAC CACGGTCTAG GAATCGGAAA CGGAATGCTC
    AGTGCCTTCC AGCCACCGCA ACACCACCAG GTTCAAATGC CACCACCGCA TCCGCAACAG
    ATTTACGCCA ATGTGCCGCA GCAGCAGCAA CAGATGGTTC TGCATGCATA TCACCAGATG
    GACACGGACT ACCGCAAGTC CATGTCGGAT CTAAACGAGT TCTCCAACTG CCTGATACTG
    CCGCCAACGC CGCCCACAAA GCCATTGAGG GCTATGCAAT TGAACGCCAA TGGCCATGGC
    CTGGAGCCAG ATTACGCTGT TAGCACGAGG CAGCGCCAAC CACCGGCTCC GTACGGTGGC
    TCTCTGGTAA AGATTGCTGG AGCACCAATG CCGCCACGGC TCCCCGGTCA AGGATATCCC
    ATTCAAGCGC CGACAGCGCC AGCATACCAT CACCAGTCGG CCCAAAACTT AAGCAATATG
    TCCAGAAACA CATTGCTCGC TTTGAGTGCC ACCCCCAAAC CAAAATACGC GGACGGATGG
    GTGCAGGTGC AGCAGCGGAA GAGTTATGAC AGCCAACAGA CCAGCGATGC GGCTTGGCTG
    GCCGCTCAGC AGCAAAAGCG CAAATCCATG CCTGACTACG GCGGAGCACT CTACAATAAC
    AACCACTGGC TACTGCAGGA GGCGGAGCAG CGACGCATCG AGCAACTTAA TCGGCGATCT
    ATACCAGCCA GCAAGTCGAT GGGCAAACCG CTGCCCGATT CCATCATACA AACTCTTACG
    GAGCGGGTCC AGAGCAAAGG AATCGGCGAT CGAAAGCGCT TCGATAGCAA CGGGAACTAT
    AGCCAGGTAA ATGGCAACAA CATCTACCAG CAGCAACAGC AGCAGAAGAA TGTGACGAAT
    GGCAGCAGCA TCAATGGCAA CAGCAGCCAG GGGCAGGAGA AGGTGCTCAG CGTTAGTGGC
    AAGAAGAAGT GCTCCCATTG CGGCGACGAA TTAGGTAAAC TTTGCTACTA CGTTGGTTAT
    CTGGGCTGCA CTCACGGCAA TTGTCGGCAG GTGCACAAAG CGCTAAAATA TATTGTTTGG
    GTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246913.1
    CDS1176..5681
    Translation

    Target ORF information:

    RefSeq Version NM_001259984.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster smallish (smash), transcript variant P, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259984.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAGGCCA CTGATCAGGA GGTCATGCTG GAGGGCAGGG AAGACCTCAT GTCCACATCG 
    GCGACATCGT CCACAAATGG CGGGGACACT AATGGTTGCG GCAAAAAATT GTCCGTAACC
    TCTCCGGATC CCACACCGTA CGTGACCAAG CAGCGAGTGA CGCGTCCAAC GCCTCCCGCT
    CCTTCAAAGA ATCCGATGCA ATTCGTCCAG ATCAAGCCCT GCAACCTCTA CCAGACCGCG
    CAGGAGCAGC TGAAGAAGGC CGAGGAGGTC AAGAAGCTGA AGGAGGTGAA GAAGGAGGAG
    CCAGAGGAGT GGCAGAACAA CCTTGACAAT TGGAAATCAT CGAGACGAAA ACGTGTCGAA
    CACATCATCG ATCGAGCGGT GGAGACCAAG AAACTCGAGC TGGAGGAGCA TGATCGGTTG
    CGCCGAAAGT CGAAAACATT CACCGAAATG ATGGAGGAGA GGGCTGAAAG GGGCGGACCT
    CGGGGGCGTG CCAAGCTGGC TTCCCTGGCC GTATACAACG AGGATGAGAC GAACGACCTG
    AGTGATTTGG GGATCGGAAC CAGCAGTGCC AGCGGAAAGA GCAGCCTGTC CGGGGACTAT
    GACAATAACA GTGTTATGAG CGACCATGCG GCGGAGCTGG ATAAGGCGAT TGGAGCAGCT
    AGCTCTGTAG CTGCTGGAGT AACCGCCCAA AACGTGGATG AGCAGCTGAA TCACATAAAC
    CGCAACGGTA GTAATGGGAA CCACGGGAAC GGAGCGGCTG TTGGCCAGAC AGGACCATCA
    AATTCCTCCA AGTCAGCAGG GCGGCAATAC ATCTCCTCGC CAGGATACGA TACGTCCTCC
    AGTAACGCGC AAGCCAGTTC TCCGGATCTC TGTGAATACA CCTACGAAGG AGCCATCCAG
    GACTACAAGC AAAGAGTTCA ACGGGCCAGC AGCAATGGCA ATGGAAATGC CAACGGCAAG
    TTGATTGGAG AACATATTGC TTATCCCACG AGGCGAGGCT CTAAAATTGA GGATCGATTG
    AGTGGTTTTG AGGTGACATC GCCAAGCGAC ACGCAGGAGG GTGTAGAGAA GCAAAAGGTG
    GACGTGCCCA AGGTTGACAT ATCCAAACGC AAGGAGATCT TCGAGCAGGC CAAGGCGGAA
    GTCTCAAACG GTGGTGCTCC AGCTGCCCCA AAACTCGTAT TTCGTGATGG TCTTACCAAT
    GGAAATTCAG CTACCACTGC TCCCAAATCG GAGGTGAGGC GTCTTTCTGG TGACATCTCC
    AGCATTCGAG ATCGCATGCA GAGTTTGGAA CAGCAGCGGA AGGCTTTCAG TTCCAGCAAG
    AGTGTGGATG TGCCGGTGCC GCCCCTTAAA CAGCGTCTGA ATAGTCTGCA GCAGTCCGTT
    GCCAAAGAGG AGCAGAAGAA GCCACCGTTG GTGGCGCTCA TCGATGCCAG ACAACTGGAG
    ATCATGAGGG GAGAAGAGGA ACGTATGCGC CAGCAGCAAC AAAAAGAGAA GCATTCACCA
    AGCCAAACTA CATTGTGTGC ACCTAAGCCA GCTCCTCCTG CCCTTATTAT TGAGGAACCG
    CCGGTGGCAG ACACCAACGA CGACAGCGGC ATTCAGGAGG ACACCGCTGA TGAACTGCAG
    CAGCAGCAGC AACTCAACGC AGCCATCGCC GCCTTGGCGC TCGAGGAGCG CCAGTTGGAG
    GAAGCAGCCA ATGCGGTCAA CCAAATTGAA GCGGAGTTCG ATGAGCTGAC TGATCTGCAT
    CCCTCGCCAT TGCCACCATC TCCAGCTCTT CCGGCGGTTC AACAAAGCCT CCAACAACCG
    ACAGTCGCTC AGGTTCCAGC ACAAGCGGTC AATCAGGCGG CTGCTCCTCC ATTGCGGGAC
    ATGGAATTTA GTATCAACGA AGCTCTTGAA TTGGCGCTGC AAGCTATTGA TCGCGAGGCG
    ACAACCAAGC CAATGGACAA GGCACAACAT CCACAGAAGC GGGAACAGAA GGAGGAGCTG
    GGAGGTAAGC CGGAGGAGAA GGAGCCAATT TGCGAGGAGC AGCACGACGA AGAGTCCAAC
    GAGCAGGGGG AAACACACAA CGAGGGTGGG GAAATGCCAA ATAAAACCGA TAAGCAAATG
    CAGGGGGGAC GAGAGCCCAT CTATGAGAAT GTCAGCTCGA CTATATCTAT GCATGAAGTA
    GATAATGAAC AGATAGCAAC TATGACGATA ACCACACAGA CCCAAGCGAC GCGAATAAGT
    CACAGGAGAA GCATTCCAAG CCAGCAAACC AGCAACAGTA ACGAGAATAA TCAAAGCACT
    AATCAAACCA GCAACAGCAG GAATCAAAAA GAGAGCAGCA CAGCAGCAAC ATCGGCAGCG
    AACCCCGTGC CACCAACAAC ACCGGGGGAA CAAGCAGAGC CCTACTACCA AGTGCCGAAG
    GCCACGGAGC CCTACTACGA TGCCCCCAAG CATTTAAGGC CGGTGCCGGT ATACGAAAAT
    GTCGAGATCT TTTACTCGGG CCTGGAGATT AGTCAGGGAT CTGTGGGAGC GCCAGTAGGG
    CTGATGGAGC CACCCAAGGA AAAGCCACCA CCACCGCCCA CCGAGAGTCC GATTCCGCTG
    GACGAGGGCC ATGACGACGA GCTGGATGGA CTGGGCAACA GTCTGGGCCA CAACACAGAC
    ACCTGGTCAT CGGACAACAC CTACGAGACC ATATCGAACG GCACTCGCCG GCACCTTCAG
    GGACAACCGG AACCCGCCCA ACCCTCGCCG CCCATCAAGA GGATGAACTC GACCAAACGG
    ATCAAGAAGG AGTTGCGCAA CAAGCGCTCC AGCTTCCTGG GCATCGAAAA CGACGGCGAT
    CTTGACGACA TGGAGACCTA TCTCGAGCTA ACAGTGGCCC CGCCTCCGGA TATGGCGCAG
    CTCTTGCAGG AGGAGCGGCG ACTGGAGAAG CAGCTGTACA TCAAGGCGGG ACTCTGCGAC
    AGTTCCGACA CAGGTGAAAG CCGCGATTCT GGAGTTTCTG AAAACCACTC TCGCCAGAGC
    AGTGAGCACT ACACGAACTC CTCTGAGGAG AACGATACAC AGTCAGAGGC CACACCGCCG
    CCATTGCCTC CACCACCATC GACCGCCCAA GTGGGTGAAG TCATATACCA AAATGAGAGC
    CTCCTGGCTG CCCAGACGCC GCTTCTCCAG ACGGTAAAAG GAAACTCCGC CGAGTCCTGG
    ACCGAATCAG CAACGGCCGC AGCGGCGGCC ACGCAATCAT TGAGCGAAGC CACAGCAACG
    GCCAATGCCA AGATGCAGTC CATCGAGGAT AAGATCCGGG AGCAGGGAGA GATGTTGCGG
    GTGGAACGCG AGCTACTGCA CTTCTCGCAA GAGGAACTGA AACGGCAACG TGAAAATCTT
    GTGCTTCGAG AAAATATTGC TCGACGAGAG TTGCAACACG GAGCCAAGAT GCTGATGTCG
    AACAACCGGC GCTCACTGCA GGATCTGCAC CACGGTCTAG GAATCGGAAA CGGAATGCTC
    AGTGCCTTCC AGCCACCGCA ACACCACCAG GTTCAAATGC CACCACCGCA TCCGCAACAG
    ATTTACGCCA ATGTGCCGCA GCAGCAGCAA CAGATGGTTC TGCATGCATA TCACCAGATG
    GACACGGACT ACCGCAAGTC CATGTCGGAT CTAAACGAGT TCTCCAACTG CCTGATACTG
    CCGCCAACGC CGCCCACAAA GCCATTGAGG GCTATGCAAT TGAACGCCAA TGGCCATGGC
    CTGGAGCCAG ATTACGCTGT TAGCACGAGG CAGCGCCAAC CACCGGCTCC GTACGGTGGC
    TCTCTGGTAA AGATTGCTGG AGCACCAATG CCGCCACGGC TCCCCGGTCA AGGATATCCC
    ATTCAAGCGC CGACAGCGCC AGCATACCAT CACCAGTCGG CCCAAAACTT AAGCAATATG
    TCCAGAAACA CATTGCTCGC TTTGAGTGCC ACCCCCAAAC CAAAATACGC GGACGGATGG
    GTGCAGGTGC AGCAGCGGAA GAGTTATGAC AGCCAACAGA CCAGCGATGC GGCTTGGCTG
    GCCGCTCAGC AGCAAAAGCG CAAATCCATG CCTGACTACG GCGGAGCACT CTACAATAAC
    AACCACTGGC TACTGCAGGA GGCGGAGCAG CGACGCATCG AGCAACTTAA TCGGCGATCT
    ATACCAGCCA GCAAGTCGAT GGGCAAACCG CTGCCCGATT CCATCATACA AACTCTTACG
    GAGCGGGTCC AGAGCAAAGG AATCGGCGAT CGAAAGCGCT TCGATAGCAA CGGGAACTAT
    AGCCAGGTAA ATGGCAACAA CATCTACCAG CAGCAACAGC AGCAGAAGAA TGTGACGAAT
    GGCAGCAGCA TCAATGGCAA CAGCAGCCAG GGGCAGGAGA AGGTGCTCAG CGTTAGTGGC
    AAGAAGAAGT GCTCCCATTG CGGCGACGAA TTAGGTAAAC TTTGCTACTA CGTTGGTTAT
    CTGGGCTGCA CTCACGGCAA TTGTCGGCAG GTGCACAAAG CGCTAAAATA TATTGTTTGG
    GTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08055
    Clone ID Related Accession (Same CDS sequence) NM_001259983.2
    Accession Version NM_001259983.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4626bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product smallish, isoform O
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001259983.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGGCCA CTGATCAGGA GGTCATGCTG GAGGGCAGGG AAGACCTCAT GTCCACATCG 
    GCGACATCGT CCACAAATGG CGGGGACACT AATGGTTGCG GCAAAAAATT GTCCGTAACC
    TCTCCGGATC CCACACCGTA CGTGACCAAG CAGCGAGTGA CGCGTCCAAC GCCTCCCGCT
    CCTTCAAAGA ATCCGATGCA ATTCGTCCAG ATCAAGCCCT GCAACCTCTA CCAGACCGCG
    CAGGAGCAGC TGAAGAAGGC CGAGGAGGTC AAGAAGCTGA AGGAGGTGAA GAAGGAGGAG
    CCAGAGGAGT GGCAGAACAA CCTTGACAAT TGGAAATCAT CGAGACGAAA ACGTGTCGAA
    CACATCATCG ATCGAGCGGT GGAGACCAAG AAACTCGAGC TGGAGGAGCA TGATCGGTTG
    CGCCGAAAGT CGAAAACATT CACCGAAATG ATGGAGGAGA GGGCTGAAAG GGGCGGACCT
    CGGGGGCGTG CCAAGCTGGC TTCCCTGGCC GTATACAACG AGGATGAGAC GAACGACCTG
    AGTGATTTGG GGATCGGAAC CAGCAGTGCC AGCGGAAAGA GCAGCCTGTC CGGGGACTAT
    GACAATAACA GTGTTATGAG CGACCATGCG GCGGAGCTGG ATAAGGCGAT TGGAGCAGCT
    AGCTCTGTAG CTGCTGGAGT AACCGCCCAA AACGTGGATG AGCAGCTGAA TCACATAAAC
    CGCAACGGTA GTAATGGGAA CCACGGGAAC GGAGCGGCTG TTGGCCAGAC AGGACCATCA
    AATTCCTCCA AGTCAGCAGG GCGGCAATAC ATCTCCTCGC CAGGATACGA TACGTCCTCC
    AGTAACGCGC AAGCCAGTTC TCCGGATCTC TGTGAATACA CCTACGAAGG AGCCATCCAG
    GACTACAAGC AAAGAGTTCA ACGGGCCAGC AGCAATGGCA ATGGAAATGC CAACGGCAAG
    TTGATTGGAG AACATATTGC TTATCCCACG AGGCGAGGCT CTAAAATTGA GGATCGATTG
    AGTGGTTTTG AGGTGACATC GCCAAGCGAC ACGCAGGAGG GTGTAGAGAA GCAAAAGGTG
    GACGTGCCCA AGGTTGACAT ATCCAAACGC AAGGAGATCT TCGAGCAGGC CAAGGCGGAA
    GTCTCAAACG GTGGTGCTCC AGCTGCCCCA AAACTCGTAT TTCGTGATGG TCTTACCAAT
    GGAAATTCAG CTACCACTGC TCCCAAATCG GAGGTGAGGC GTCTTTCTGG TGACATCTCC
    AGCATTCGAG ATCGCATGCA GAGTTTGGAA CAGCAGCGGA AGGCTTTCAG TTCCAGCAAG
    AGTGTGGATG TGCCGGTGCC GCCCCTTAAA CAGCGTCTGA ATAGTCTGCA GCAGTCCGTT
    GCCAAAGAGG AGCAGAAGAA GCCACCGTTG GTGGCGCTCA TCGATGCCAG ACAACTGGAG
    ATCATGAGGG GAGAAGAGGA ACGTATGCGC CAGCAGCAAC AAAAAGAGAA GCATTCACCA
    AGCCAAACTA CATTGTGTGC ACCTAAGCCA GCTCCTCCTG CCCTTATTAT TGAGGAACCG
    CCGGTGGCAG ACACCAACGA CGACAGCGGC ATTCAGGAGG ACACCGCTGA TGAACTGCAG
    CAGCAGCAGC AACTCAACGC AGCCATCGCC GCCTTGGCGC TCGAGGAGCG CCAGTTGGAG
    GAAGCAGCCA ATGCGGTCAA CCAAATTGAA GCGGAGTTCG ATGAGCTGAC TGATCTGCAT
    CCCTCGCCAT TGCCACCATC TCCAGCTCTT CCGGCGGTTC AACAAAGCCT CCAACAACCG
    ACAGTCGCTC AGGTTCCAGC ACAAGCGGTC AATCAGGCGG CTGCTCCTCC ATTGCGGGAC
    ATGGAATTTA GTATCAACGA AGCTCTTGAA TTGGCGCTGC AAGCTATTGA TCGCGAGGCG
    ACAACCAAGC CAATGGACAA GGCACAACAT CCACAGAAGC GGGAACAGAA GGAGGAGCTG
    GGAGGTAAGC CGGAGGAGAA GGAGCCAATT TGCGAGGAGC AGCACGACGA AGAGTCCAAC
    GAGCAGGGGG AAACACACAA CGAGGGTGGG GAAATGCCAA ATAAAACCGA TAAGCAAATG
    CAGGGGGGAC GAGAGCCCAT CTATGAGAAT GTCAGCTCGA CTATATCTAT GCATGAAGTA
    GATAATGAAC AGATAGCAAC TATGACGATA ACCACACAGA CCCAAGCGAC GCGAATAAGT
    CACAGGAGAA GCATTCCAAG CCAGCAAACC AGCAACAGTA ACGAGAATAA TCAAAGCACT
    AATCAAACCA GCAACAGCAG GAATCAAAAA GAGAGCAGCA CAGCAGCAAC ATCGGCAGCG
    AACCCCGTGC CACCAACAAC ACCGGGGGAA CAAGCAGAGC CCTACTACCA AGTGCCGAAG
    GCCACGGAGC CCTACTACGA TGCCCCCAAG CATTTAAGGC CGGTGCCGGT ATACGAAAAT
    GTCGAGATCT TTTACTCGGG CCTGGAGATT AGTCAGGGAT CTGTGGGAGC GCCAGTAGGG
    CTGATGGAGC CACCCAAGGA AAAGCCACCA CCACCGCCCA CCGAGAGTCC GATTCCGCTG
    GACGAGGGCC ATGACGACGA GCTGGATGGA CTGGGCAACA GTCTGGGCCA CAACACAGAC
    ACCTGGTCAT CGGACAACAC CTACGAGACC ATATCGAACG GCACTCGCCG GCACCTTCAG
    GGACAACCGG AACCCGCCCA ACCCTCGCCG CCCATCAAGA GGATGAACTC GACCAAACGG
    ATCAAGAAGG AGTTGCGCAA CAAGCGCTCC AGCTTCCTGG GCATCGAAAA CGACGGCGAT
    CTTGACGACA TGGAGACCTA TCTCGAGCTA ACAGTGGCCC CGCCTCCGGA TATGGCGCAG
    CTCTTGCAGG AGGAGCGGCG ACTGGAGAAG CAGCTGTACA TCAAGGCGGG ACTCTGCGAC
    AGTTCCGACA CAGGTGAAAG CCGCGATTCT GGAGTTTCTG AAAACCACTC TCGCCAGAGC
    AGTGAGCACT ACACGAACTC CTCTGAGGAG AACGATACAC AGTCAGAGGC CACACCGCCG
    CCATTGCCTC CACCACCATC GACCGCCCAA GTGGGTGAAG TCATATACCA AAATGAGAGC
    CTCCTGGCTG CCCAGACGCC GCTTCTCCAG ACGGTAAAAG GAAACTCCGC CGAGTCCTGG
    ACCGAATCAG CAACGGCCGC AGCGGCGGCC ACGCAATCAT TGAGCGAAGC CACAGCAACG
    GCCAATGCCA AGATGCAGTC CATCGAGGAT AAGATCCGGG AGCAGGGAGA GATGTTGCGG
    GTGGAACGCG AGCTACTGCA CTTCTCGCAA GAGGAACTGA AACGGCAACG TGAAAATCTT
    GTGCTTCGAG AAAATATTGC TCGACGAGAG TTGCAACACG GAGCCAAGAT GCTGATGTCG
    AACAACCGGC GCTCACTGCA GGATCTGCAC CACGGTCTAG GAATCGGAAA CGGAATGCTC
    AGTGCCTTCC AGCCACCGCA ACACCACCAG GTTCAAATGC CACCACCGCA TCCGCAACAG
    ATTTACGCCA ATGTGCCGCA GCAGCAGCAA CAGATGGTTC TGCATGCATA TCACCAGATG
    GACACGGACT ACCGCAAGTC CATGTCGGAT CTAAACGAGT TCTCCAACTG CCTGATACTG
    CCGCCAACGC CGCCCACAAA GCCATTGAGG GCTATGCAAT TGAACGCCAA TGGCCATGGC
    CTGGAGCCAG ATTACGCTGT TAGCACGAGG CAGCGCCAAC CACCGGCTCC GTACGGTGGC
    TCTCTGGTAA AGATTGCTGG AGCACCAATG CCGCCACGGC TCCCCGGTCA AGGATATCCC
    ATTCAAGCGC CGACAGCGCC AGCATACCAT CACCAGTCGG CCCAAAACTT AAGCAATATG
    TCCAGAAACA CATTGCTCGC TTTGAGTGCC ACCCCCAAAC CAAAATACGC GGACGGATGG
    GTGCAGGTGC AGCAGCGGAA GAGTTATGAC AGCCAACAGA CCAGCGATGC GGCTTGGCTG
    GCCGCTCAGC AGCAAAAGCG CAAATCCATG CCTGACTACG GCGGAGCACT CTACAATAAC
    AACCACTGGC TACTGCAGGA GGCGGAGCAG CGACGCATCG AGCAACTTAA TCGGCGATCT
    ATACCAGCCA GCAAGTCGAT GGGCAAACCG CTGCCCGATT CCATCATACA AACTCTTACG
    GAGCGGGTCC AGAGCAAAGG AATCGGCGAT CGAAAGCGCT TCGATAGCAA CGGGAACTAT
    AGCCAGGTAA ATGGCAACAA CATCTACCAG CAGCAACAGC AGCAGAAGAA TGTGACGAAT
    GGCAGCAGCA TCAATGGCAA CAGCAGCCAG GGGCAGGAGA AGGTGCTCAG CGTTAGTGGC
    AAGAAGAAGT GCTCCCATTG CGGCGACGAA TTAGGTAAAC TTTGCTACTA CGTTGGTTGT
    CGCGGCGCTG CCATGATCAT CGAATCGCTT CTGCTGTTCT ACCACATCAA CTGCTTCAAG
    TGTTGCGTCT GCCACGTCCA GCTGGGAGAT GGCCTCAATG GAACCGATGT CCGGGTGCGA
    AACCACAAGC TGCATTGCCA AAACTGCTAC TCCAGCGATG ACGGCATTAA GTTCAGCTGC
    GTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001246912.1
    CDS1527..6152
    Translation

    Target ORF information:

    RefSeq Version NM_001259983.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster smallish (smash), transcript variant O, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001259983.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGGCCA CTGATCAGGA GGTCATGCTG GAGGGCAGGG AAGACCTCAT GTCCACATCG 
    GCGACATCGT CCACAAATGG CGGGGACACT AATGGTTGCG GCAAAAAATT GTCCGTAACC
    TCTCCGGATC CCACACCGTA CGTGACCAAG CAGCGAGTGA CGCGTCCAAC GCCTCCCGCT
    CCTTCAAAGA ATCCGATGCA ATTCGTCCAG ATCAAGCCCT GCAACCTCTA CCAGACCGCG
    CAGGAGCAGC TGAAGAAGGC CGAGGAGGTC AAGAAGCTGA AGGAGGTGAA GAAGGAGGAG
    CCAGAGGAGT GGCAGAACAA CCTTGACAAT TGGAAATCAT CGAGACGAAA ACGTGTCGAA
    CACATCATCG ATCGAGCGGT GGAGACCAAG AAACTCGAGC TGGAGGAGCA TGATCGGTTG
    CGCCGAAAGT CGAAAACATT CACCGAAATG ATGGAGGAGA GGGCTGAAAG GGGCGGACCT
    CGGGGGCGTG CCAAGCTGGC TTCCCTGGCC GTATACAACG AGGATGAGAC GAACGACCTG
    AGTGATTTGG GGATCGGAAC CAGCAGTGCC AGCGGAAAGA GCAGCCTGTC CGGGGACTAT
    GACAATAACA GTGTTATGAG CGACCATGCG GCGGAGCTGG ATAAGGCGAT TGGAGCAGCT
    AGCTCTGTAG CTGCTGGAGT AACCGCCCAA AACGTGGATG AGCAGCTGAA TCACATAAAC
    CGCAACGGTA GTAATGGGAA CCACGGGAAC GGAGCGGCTG TTGGCCAGAC AGGACCATCA
    AATTCCTCCA AGTCAGCAGG GCGGCAATAC ATCTCCTCGC CAGGATACGA TACGTCCTCC
    AGTAACGCGC AAGCCAGTTC TCCGGATCTC TGTGAATACA CCTACGAAGG AGCCATCCAG
    GACTACAAGC AAAGAGTTCA ACGGGCCAGC AGCAATGGCA ATGGAAATGC CAACGGCAAG
    TTGATTGGAG AACATATTGC TTATCCCACG AGGCGAGGCT CTAAAATTGA GGATCGATTG
    AGTGGTTTTG AGGTGACATC GCCAAGCGAC ACGCAGGAGG GTGTAGAGAA GCAAAAGGTG
    GACGTGCCCA AGGTTGACAT ATCCAAACGC AAGGAGATCT TCGAGCAGGC CAAGGCGGAA
    GTCTCAAACG GTGGTGCTCC AGCTGCCCCA AAACTCGTAT TTCGTGATGG TCTTACCAAT
    GGAAATTCAG CTACCACTGC TCCCAAATCG GAGGTGAGGC GTCTTTCTGG TGACATCTCC
    AGCATTCGAG ATCGCATGCA GAGTTTGGAA CAGCAGCGGA AGGCTTTCAG TTCCAGCAAG
    AGTGTGGATG TGCCGGTGCC GCCCCTTAAA CAGCGTCTGA ATAGTCTGCA GCAGTCCGTT
    GCCAAAGAGG AGCAGAAGAA GCCACCGTTG GTGGCGCTCA TCGATGCCAG ACAACTGGAG
    ATCATGAGGG GAGAAGAGGA ACGTATGCGC CAGCAGCAAC AAAAAGAGAA GCATTCACCA
    AGCCAAACTA CATTGTGTGC ACCTAAGCCA GCTCCTCCTG CCCTTATTAT TGAGGAACCG
    CCGGTGGCAG ACACCAACGA CGACAGCGGC ATTCAGGAGG ACACCGCTGA TGAACTGCAG
    CAGCAGCAGC AACTCAACGC AGCCATCGCC GCCTTGGCGC TCGAGGAGCG CCAGTTGGAG
    GAAGCAGCCA ATGCGGTCAA CCAAATTGAA GCGGAGTTCG ATGAGCTGAC TGATCTGCAT
    CCCTCGCCAT TGCCACCATC TCCAGCTCTT CCGGCGGTTC AACAAAGCCT CCAACAACCG
    ACAGTCGCTC AGGTTCCAGC ACAAGCGGTC AATCAGGCGG CTGCTCCTCC ATTGCGGGAC
    ATGGAATTTA GTATCAACGA AGCTCTTGAA TTGGCGCTGC AAGCTATTGA TCGCGAGGCG
    ACAACCAAGC CAATGGACAA GGCACAACAT CCACAGAAGC GGGAACAGAA GGAGGAGCTG
    GGAGGTAAGC CGGAGGAGAA GGAGCCAATT TGCGAGGAGC AGCACGACGA AGAGTCCAAC
    GAGCAGGGGG AAACACACAA CGAGGGTGGG GAAATGCCAA ATAAAACCGA TAAGCAAATG
    CAGGGGGGAC GAGAGCCCAT CTATGAGAAT GTCAGCTCGA CTATATCTAT GCATGAAGTA
    GATAATGAAC AGATAGCAAC TATGACGATA ACCACACAGA CCCAAGCGAC GCGAATAAGT
    CACAGGAGAA GCATTCCAAG CCAGCAAACC AGCAACAGTA ACGAGAATAA TCAAAGCACT
    AATCAAACCA GCAACAGCAG GAATCAAAAA GAGAGCAGCA CAGCAGCAAC ATCGGCAGCG
    AACCCCGTGC CACCAACAAC ACCGGGGGAA CAAGCAGAGC CCTACTACCA AGTGCCGAAG
    GCCACGGAGC CCTACTACGA TGCCCCCAAG CATTTAAGGC CGGTGCCGGT ATACGAAAAT
    GTCGAGATCT TTTACTCGGG CCTGGAGATT AGTCAGGGAT CTGTGGGAGC GCCAGTAGGG
    CTGATGGAGC CACCCAAGGA AAAGCCACCA CCACCGCCCA CCGAGAGTCC GATTCCGCTG
    GACGAGGGCC ATGACGACGA GCTGGATGGA CTGGGCAACA GTCTGGGCCA CAACACAGAC
    ACCTGGTCAT CGGACAACAC CTACGAGACC ATATCGAACG GCACTCGCCG GCACCTTCAG
    GGACAACCGG AACCCGCCCA ACCCTCGCCG CCCATCAAGA GGATGAACTC GACCAAACGG
    ATCAAGAAGG AGTTGCGCAA CAAGCGCTCC AGCTTCCTGG GCATCGAAAA CGACGGCGAT
    CTTGACGACA TGGAGACCTA TCTCGAGCTA ACAGTGGCCC CGCCTCCGGA TATGGCGCAG
    CTCTTGCAGG AGGAGCGGCG ACTGGAGAAG CAGCTGTACA TCAAGGCGGG ACTCTGCGAC
    AGTTCCGACA CAGGTGAAAG CCGCGATTCT GGAGTTTCTG AAAACCACTC TCGCCAGAGC
    AGTGAGCACT ACACGAACTC CTCTGAGGAG AACGATACAC AGTCAGAGGC CACACCGCCG
    CCATTGCCTC CACCACCATC GACCGCCCAA GTGGGTGAAG TCATATACCA AAATGAGAGC
    CTCCTGGCTG CCCAGACGCC GCTTCTCCAG ACGGTAAAAG GAAACTCCGC CGAGTCCTGG
    ACCGAATCAG CAACGGCCGC AGCGGCGGCC ACGCAATCAT TGAGCGAAGC CACAGCAACG
    GCCAATGCCA AGATGCAGTC CATCGAGGAT AAGATCCGGG AGCAGGGAGA GATGTTGCGG
    GTGGAACGCG AGCTACTGCA CTTCTCGCAA GAGGAACTGA AACGGCAACG TGAAAATCTT
    GTGCTTCGAG AAAATATTGC TCGACGAGAG TTGCAACACG GAGCCAAGAT GCTGATGTCG
    AACAACCGGC GCTCACTGCA GGATCTGCAC CACGGTCTAG GAATCGGAAA CGGAATGCTC
    AGTGCCTTCC AGCCACCGCA ACACCACCAG GTTCAAATGC CACCACCGCA TCCGCAACAG
    ATTTACGCCA ATGTGCCGCA GCAGCAGCAA CAGATGGTTC TGCATGCATA TCACCAGATG
    GACACGGACT ACCGCAAGTC CATGTCGGAT CTAAACGAGT TCTCCAACTG CCTGATACTG
    CCGCCAACGC CGCCCACAAA GCCATTGAGG GCTATGCAAT TGAACGCCAA TGGCCATGGC
    CTGGAGCCAG ATTACGCTGT TAGCACGAGG CAGCGCCAAC CACCGGCTCC GTACGGTGGC
    TCTCTGGTAA AGATTGCTGG AGCACCAATG CCGCCACGGC TCCCCGGTCA AGGATATCCC
    ATTCAAGCGC CGACAGCGCC AGCATACCAT CACCAGTCGG CCCAAAACTT AAGCAATATG
    TCCAGAAACA CATTGCTCGC TTTGAGTGCC ACCCCCAAAC CAAAATACGC GGACGGATGG
    GTGCAGGTGC AGCAGCGGAA GAGTTATGAC AGCCAACAGA CCAGCGATGC GGCTTGGCTG
    GCCGCTCAGC AGCAAAAGCG CAAATCCATG CCTGACTACG GCGGAGCACT CTACAATAAC
    AACCACTGGC TACTGCAGGA GGCGGAGCAG CGACGCATCG AGCAACTTAA TCGGCGATCT
    ATACCAGCCA GCAAGTCGAT GGGCAAACCG CTGCCCGATT CCATCATACA AACTCTTACG
    GAGCGGGTCC AGAGCAAAGG AATCGGCGAT CGAAAGCGCT TCGATAGCAA CGGGAACTAT
    AGCCAGGTAA ATGGCAACAA CATCTACCAG CAGCAACAGC AGCAGAAGAA TGTGACGAAT
    GGCAGCAGCA TCAATGGCAA CAGCAGCCAG GGGCAGGAGA AGGTGCTCAG CGTTAGTGGC
    AAGAAGAAGT GCTCCCATTG CGGCGACGAA TTAGGTAAAC TTTGCTACTA CGTTGGTTGT
    CGCGGCGCTG CCATGATCAT CGAATCGCTT CTGCTGTTCT ACCACATCAA CTGCTTCAAG
    TGTTGCGTCT GCCACGTCCA GCTGGGAGAT GGCCTCAATG GAACCGATGT CCGGGTGCGA
    AACCACAAGC TGCATTGCCA AAACTGCTAC TCCAGCGATG ACGGCATTAA GTTCAGCTGC
    GTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08057
    Clone ID Related Accession (Same CDS sequence) NM_001144532.3
    Accession Version NM_001144532.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product smallish, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001144532.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGGCCA CTGATCAGGA GGTCATGCTG GAGGGCAGGG AAGACCTCAT GTCCACATCG 
    GCGACATCGT CCACAAATGG CGGGGACACT AATGGTTGCG GCAAAAAATT GTCCGTAACC
    TCTCCGGATC CCACACCGTA CGTGACCAAG CAGCGAGTGA CGCGTCCAAC GCCTCCCGCT
    CCTTCAAAGA ATCCGATGCA ATTCGTCCAG ATCAAGCCCT GCAACCTCTA CCAGACCGCG
    CAGGAGCAGC TGAAGAAGGC CGAGGAGGTC AAGAAGCTGA AGGAGGTGAA GAAGGAGGAG
    CCAGAGGAGT GGCAGAACAA CCTTGACAAT TGGAAATCAT CGAGACGAAA ACGTGTCGAA
    CACATCATCG ATCGAGCGGT GGAGACCAAG AAACTCGAGC TGGAGGAGCA TGATCGGTTG
    CGCCGAAAGT CGAAAACATT CACCGAAATG ATGGAGGAGA GGGCTGAAAG GGGCGGACCT
    CGGGGGCGTG CCAAGCTGGC TTCCCTGGCC GTATACAACG AGGATGAGAC GAACGACCTG
    AGTGATTTGG GGATCGGAAC CAGCAGTGCC AGCGGAAAGA GCAGCCTGTC CGGGGACTAT
    GACAATAACA GTGTTATGAG CGACCATGCG GCGGAGCTGG ATAAGGCGAT TGGAGCAGCT
    AGCTCTGTAG CTGCTGGAGT AACCGCCCAA AACGTGGATG AGCAGCTGAA TCACATAAAC
    CGCAACGGTA GTAATGGGAA CCACGGGAAC GGAGCGGCTG TTGGCCAGAC AGGACCATCA
    AATTCCTCCA AGTCAGCAGG GCGGCAATAC ATCTCCTCGC CAGGATACGA TACGTCCTCC
    AGTAACGCGC AAGCCAGTTC TCCGGATCTC TGTGAATACA CCTACGAAGG AGCCATCCAG
    GACTACAAGC AAAGAGTTCA ACGGGCCAGC AGCAATGGCA ATGGAAATGC CAACGGCAAG
    TTGATTGGAG AACATATTGC TTATCCCACG AGGCGAGGCT CTAAAATTGA GGATCGATTG
    AGTGGTTTTG AGGTGACATC GCCAAGCGAC ACGCAGGAGG GTGTAGAGAA GCAAAAGGTG
    GACGTGCCCA AGGTTGACAT ATCCAAACGC AAGGAGATCT TCGAGCAGGC CAAGGCGGAA
    GTCTCAAACG GTGGTGCTCC AGCTGCCCCA AAACTCGTAT TTCGTGATGG TCTTACCAAT
    GGAAATTCAG CTACCACTGC TCCCAAATCG GAGGTGAGGC GTCTTTCTGG TGACATCTCC
    AGCATTCGAG ATCGCATGCA GAGTTTGGAA CAGCAGCGGA AGGCTTTCAG TTCCAGCAAG
    AGTGTGGATG TGCCGGTGCC GCCCCTTAAA CAGCGTCTGA ATAGTCTGCA GCAGTCCGTT
    GCCAAAGAGG AGCAGAAGAA GCCACCGTTG GTGGCGCTCA TCGATGCCAG ACAACTGGAG
    ATCATGAGGG GAGAAGAGGA ACGTATGCGC CAGCAGCAAC AAAAAGAGAA GCATTCACCA
    AGCCAAACTA CATTGTGTGC ACCTAAGCCA GCTCCTCCTG CCCTTATTAT TGAGGAACCG
    CCGGTGGCAG ACACCAACGA CGACAGCGGC ATTCAGGAGG ACACCGCTGA TGAACTGCAG
    CAGCAGCAGC AACTCAACGC AGCCATCGCC GCCTTGGCGC TCGAGGAGCG CCAGTTGGAG
    GAAGCAGCCA ATGCGGTCAA CCAAATTGAA GCGGAGTTCG ATGAGCTGAC TGATCTGCAT
    CCCTCGCCAT TGCCACCATC TCCAGCTCTT CCGGCGGTTC AACAAAGCCT CCAACAACCG
    ACAGTCGCTC AGGTTCCAGC ACAAGCGGTC AATCAGGCGG CTGCTCCTCC ATTGCGGGAC
    ATGGAATTTA GTATCAACGA AGCTCTTGAA TTGGCGCTGC AAGCTATTGA TCGCGAGGCG
    ACAACCAAGC CAATGGACAA GGCACAACAT CCACAGAAGC GGGAACAGAA GGAGGAGCTG
    GGAGGTAAGC CGGAGGAGAA GGAGCCAATT TGCGAGGAGC AGCACGACGA AGAGTCCAAC
    GAGCAGGGGG AAACACACAA CGAGGGTGGG GAAATGCCAA ATAAAACCGA TAAGCAAATG
    CAGGGGGGAC GAGAGCCCAT CTATGAGAAT GTCAGCTCGA CTATATCTAT GCATGAAGTA
    GATAATGAAC AGATAGCAAC TATGACGATA ACCACACAGA CCCAAGCGAC GCGAATAAGT
    CACAGGAGAA GCATTCCAAG CCAGCAAACC AGCAACAGTA ACGAGAATAA TCAAAGCACT
    AATCAAACCA GCAACAGCAG GAATCAAAAA GAGAGCAGCA CAGCAGCAAC ATCGGCAGCG
    AACCCCGTGC CACCAACAAC ACCGGGGGAA CAAGCAGAGC CCTACTACCA AGTGCCGAAG
    GCCACGGAGC CCTACTACGA TGCCCCCAAG CATTTAAGGC CGGTGCCGGT ATACGAAAAT
    GTCGAGATCT TTTACTCGGG CCTGGAGATT AGTCAGGGAT CTGTGGGAGC GCCAGTAGGG
    CTGATGGAGC CACCCAAGGA AAAGCCACCA CCACCGCCCA CCGAGAGTCC GATTCCGCTG
    GACGAGGGCC ATGACGACGA GCTGGATGGA CTGGGCAACA GTCTGGGCCA CAACACAGAC
    ACCTGGTCAT CGGACAACAC CTACGAGACC ATATCGAACG GCACTCGCCG GCACCTTCAG
    GGACAACCGG AACCCGCCCA ACCCTCGCCG CCCATCAAGA GGATGAACTC GACCAAACGG
    ATCAAGAAGG AGTTGCGCAA CAAGCGCTCC AGCTTCCTGG GCATCGAAAA CGACGGCGAT
    CTTGACGACA TGGAGACCTA TCTCGAGCTA ACAGTGGCCC CGCCTCCGGA TATGGCGCAG
    CTCTTGCAGG AGGAGCGGCG ACTGGAGAAG CAGCTGTACA TCAAGGCGGG ACTCTGCGAC
    AGTTCCGACA CAGGTGAAAG CCGCGATTCT GGAGTTTCTG AAAACCACTC TCGCCAGAGC
    AGTGAGCACT ACACGAACTC CTCTGAGGAG AACGATACAC AGTCAGAGGC CACACCGCCG
    CCATTGCCTC CACCACCATC GACCGCCCAA GTGGGTGAAG TCATATACCA AAATGAGAGC
    CTCCTGGCTG CCCAGACGCC GCTTCTCCAG ACGGTAAAAG GAAACTCCGC CGAGTCCTGG
    ACCGAATCAG CAACGGCCGC AGCGGCGGCC ACGCAATCAT TGAGCGAAGC CACAGCAACG
    GCCAATGCCA AGATGCAGTC CATCGAGGAT AAGATCCGGG AGCAGGGAGA GATGTTGCGG
    GTGGAACGCG AGCTACTGCA CTTCTCGCAA GAGGAACTGA AACGGCAACG TGAAAATCTT
    GTGCTTCGAG AAAATATTGC TCGACGAGAG TTGCAACACG GAGCCAAGAT GCTGATGTCG
    AACAACCGGC GCTCACTGCA GGATCTGCAC CACGGTCTAG GAATCGGAAA CGGAATGCTC
    AGTGCCTTCC AGCCACCGCA ACACCACCAG GTTCAAATGC CACCACCGCA TCCGCAACAG
    ATTTACGCCA ATGTGCCGCA GCAGCAGCAA CAGATGGTTC TGCATGCATA TCACCAGATG
    GACACGGACT ACCGCAAGTC CATGTCGGAT CTAAACGAGT TCTCCAACTG CCTGATACTG
    CCGCCAACGC CGCCCACAAA GCCATTGAGG GCTATGCAAT TGAACGCCAA TGGCCATGGC
    CTGGAGCCAG ATTACGCTGT TAGCACGAGG CAGCGCCAAC CACCGGCTCC GTACGGTGGC
    TCTCTGGTAA AGATTGCTGG AGCACCAATG CCGCCACGGC TCCCCGGTCA AGGATATCCC
    ATTCAAGCGC CGACAGCGCC AGCATACCAT CACCAGTCGG CCCAAAACTT AAGCAATATG
    TCCAGAAACA CATTGCTCGC TTTGAGTGCC ACCCCCAAAC CAAAATACGC GGACGGATGG
    GTGCAGGTGC AGCAGCGGAA GAGTTATGAC AGCCAACAGA CCAGCGATGC GGCTTGGCTG
    GCCGCTCAGC AGCAAAAGCG CAAATCCATG CCTGACTACG GCGGAGCACT CTACAATAAC
    AACCACTGGC TACTGCAGGA GGCGGAGCAG CGACGCATCG AGCAACTTAA TCGGCGATCT
    ATACCAGCCA GCAAGTCGAT GGGCAAACCG CTGCCCGATT CCATCATACA AACTCTTACG
    GAGCGGGTCC AGAGCAAAGG AATCGGCGAT CGAAAGCGCT TCGATAGCAA CGGGAACTAT
    AGCCAGGTAA ATGGCAACAA CATCTACCAG CAGCAACAGC AGCAGAAGAA TGTGACGAAT
    GGCAGCAGCA TCAATGGCAA CAGCAGCCAG GGGCAGGAGA AGGTGCTCAG CGTTAGTGGC
    AAGAAGAAGT GCTCCCATTG CGGCGACGAA TTAGATCTGG GCTGCACTCA CGGCAATTGT
    CGGCAGGTGC ACAAAGCGCT AAAATATATT GTTTGGGTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138004.2
    CDS614..5095
    Translation

    Target ORF information:

    RefSeq Version NM_001144532.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster smallish (smash), transcript variant H, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144532.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGGCCA CTGATCAGGA GGTCATGCTG GAGGGCAGGG AAGACCTCAT GTCCACATCG 
    GCGACATCGT CCACAAATGG CGGGGACACT AATGGTTGCG GCAAAAAATT GTCCGTAACC
    TCTCCGGATC CCACACCGTA CGTGACCAAG CAGCGAGTGA CGCGTCCAAC GCCTCCCGCT
    CCTTCAAAGA ATCCGATGCA ATTCGTCCAG ATCAAGCCCT GCAACCTCTA CCAGACCGCG
    CAGGAGCAGC TGAAGAAGGC CGAGGAGGTC AAGAAGCTGA AGGAGGTGAA GAAGGAGGAG
    CCAGAGGAGT GGCAGAACAA CCTTGACAAT TGGAAATCAT CGAGACGAAA ACGTGTCGAA
    CACATCATCG ATCGAGCGGT GGAGACCAAG AAACTCGAGC TGGAGGAGCA TGATCGGTTG
    CGCCGAAAGT CGAAAACATT CACCGAAATG ATGGAGGAGA GGGCTGAAAG GGGCGGACCT
    CGGGGGCGTG CCAAGCTGGC TTCCCTGGCC GTATACAACG AGGATGAGAC GAACGACCTG
    AGTGATTTGG GGATCGGAAC CAGCAGTGCC AGCGGAAAGA GCAGCCTGTC CGGGGACTAT
    GACAATAACA GTGTTATGAG CGACCATGCG GCGGAGCTGG ATAAGGCGAT TGGAGCAGCT
    AGCTCTGTAG CTGCTGGAGT AACCGCCCAA AACGTGGATG AGCAGCTGAA TCACATAAAC
    CGCAACGGTA GTAATGGGAA CCACGGGAAC GGAGCGGCTG TTGGCCAGAC AGGACCATCA
    AATTCCTCCA AGTCAGCAGG GCGGCAATAC ATCTCCTCGC CAGGATACGA TACGTCCTCC
    AGTAACGCGC AAGCCAGTTC TCCGGATCTC TGTGAATACA CCTACGAAGG AGCCATCCAG
    GACTACAAGC AAAGAGTTCA ACGGGCCAGC AGCAATGGCA ATGGAAATGC CAACGGCAAG
    TTGATTGGAG AACATATTGC TTATCCCACG AGGCGAGGCT CTAAAATTGA GGATCGATTG
    AGTGGTTTTG AGGTGACATC GCCAAGCGAC ACGCAGGAGG GTGTAGAGAA GCAAAAGGTG
    GACGTGCCCA AGGTTGACAT ATCCAAACGC AAGGAGATCT TCGAGCAGGC CAAGGCGGAA
    GTCTCAAACG GTGGTGCTCC AGCTGCCCCA AAACTCGTAT TTCGTGATGG TCTTACCAAT
    GGAAATTCAG CTACCACTGC TCCCAAATCG GAGGTGAGGC GTCTTTCTGG TGACATCTCC
    AGCATTCGAG ATCGCATGCA GAGTTTGGAA CAGCAGCGGA AGGCTTTCAG TTCCAGCAAG
    AGTGTGGATG TGCCGGTGCC GCCCCTTAAA CAGCGTCTGA ATAGTCTGCA GCAGTCCGTT
    GCCAAAGAGG AGCAGAAGAA GCCACCGTTG GTGGCGCTCA TCGATGCCAG ACAACTGGAG
    ATCATGAGGG GAGAAGAGGA ACGTATGCGC CAGCAGCAAC AAAAAGAGAA GCATTCACCA
    AGCCAAACTA CATTGTGTGC ACCTAAGCCA GCTCCTCCTG CCCTTATTAT TGAGGAACCG
    CCGGTGGCAG ACACCAACGA CGACAGCGGC ATTCAGGAGG ACACCGCTGA TGAACTGCAG
    CAGCAGCAGC AACTCAACGC AGCCATCGCC GCCTTGGCGC TCGAGGAGCG CCAGTTGGAG
    GAAGCAGCCA ATGCGGTCAA CCAAATTGAA GCGGAGTTCG ATGAGCTGAC TGATCTGCAT
    CCCTCGCCAT TGCCACCATC TCCAGCTCTT CCGGCGGTTC AACAAAGCCT CCAACAACCG
    ACAGTCGCTC AGGTTCCAGC ACAAGCGGTC AATCAGGCGG CTGCTCCTCC ATTGCGGGAC
    ATGGAATTTA GTATCAACGA AGCTCTTGAA TTGGCGCTGC AAGCTATTGA TCGCGAGGCG
    ACAACCAAGC CAATGGACAA GGCACAACAT CCACAGAAGC GGGAACAGAA GGAGGAGCTG
    GGAGGTAAGC CGGAGGAGAA GGAGCCAATT TGCGAGGAGC AGCACGACGA AGAGTCCAAC
    GAGCAGGGGG AAACACACAA CGAGGGTGGG GAAATGCCAA ATAAAACCGA TAAGCAAATG
    CAGGGGGGAC GAGAGCCCAT CTATGAGAAT GTCAGCTCGA CTATATCTAT GCATGAAGTA
    GATAATGAAC AGATAGCAAC TATGACGATA ACCACACAGA CCCAAGCGAC GCGAATAAGT
    CACAGGAGAA GCATTCCAAG CCAGCAAACC AGCAACAGTA ACGAGAATAA TCAAAGCACT
    AATCAAACCA GCAACAGCAG GAATCAAAAA GAGAGCAGCA CAGCAGCAAC ATCGGCAGCG
    AACCCCGTGC CACCAACAAC ACCGGGGGAA CAAGCAGAGC CCTACTACCA AGTGCCGAAG
    GCCACGGAGC CCTACTACGA TGCCCCCAAG CATTTAAGGC CGGTGCCGGT ATACGAAAAT
    GTCGAGATCT TTTACTCGGG CCTGGAGATT AGTCAGGGAT CTGTGGGAGC GCCAGTAGGG
    CTGATGGAGC CACCCAAGGA AAAGCCACCA CCACCGCCCA CCGAGAGTCC GATTCCGCTG
    GACGAGGGCC ATGACGACGA GCTGGATGGA CTGGGCAACA GTCTGGGCCA CAACACAGAC
    ACCTGGTCAT CGGACAACAC CTACGAGACC ATATCGAACG GCACTCGCCG GCACCTTCAG
    GGACAACCGG AACCCGCCCA ACCCTCGCCG CCCATCAAGA GGATGAACTC GACCAAACGG
    ATCAAGAAGG AGTTGCGCAA CAAGCGCTCC AGCTTCCTGG GCATCGAAAA CGACGGCGAT
    CTTGACGACA TGGAGACCTA TCTCGAGCTA ACAGTGGCCC CGCCTCCGGA TATGGCGCAG
    CTCTTGCAGG AGGAGCGGCG ACTGGAGAAG CAGCTGTACA TCAAGGCGGG ACTCTGCGAC
    AGTTCCGACA CAGGTGAAAG CCGCGATTCT GGAGTTTCTG AAAACCACTC TCGCCAGAGC
    AGTGAGCACT ACACGAACTC CTCTGAGGAG AACGATACAC AGTCAGAGGC CACACCGCCG
    CCATTGCCTC CACCACCATC GACCGCCCAA GTGGGTGAAG TCATATACCA AAATGAGAGC
    CTCCTGGCTG CCCAGACGCC GCTTCTCCAG ACGGTAAAAG GAAACTCCGC CGAGTCCTGG
    ACCGAATCAG CAACGGCCGC AGCGGCGGCC ACGCAATCAT TGAGCGAAGC CACAGCAACG
    GCCAATGCCA AGATGCAGTC CATCGAGGAT AAGATCCGGG AGCAGGGAGA GATGTTGCGG
    GTGGAACGCG AGCTACTGCA CTTCTCGCAA GAGGAACTGA AACGGCAACG TGAAAATCTT
    GTGCTTCGAG AAAATATTGC TCGACGAGAG TTGCAACACG GAGCCAAGAT GCTGATGTCG
    AACAACCGGC GCTCACTGCA GGATCTGCAC CACGGTCTAG GAATCGGAAA CGGAATGCTC
    AGTGCCTTCC AGCCACCGCA ACACCACCAG GTTCAAATGC CACCACCGCA TCCGCAACAG
    ATTTACGCCA ATGTGCCGCA GCAGCAGCAA CAGATGGTTC TGCATGCATA TCACCAGATG
    GACACGGACT ACCGCAAGTC CATGTCGGAT CTAAACGAGT TCTCCAACTG CCTGATACTG
    CCGCCAACGC CGCCCACAAA GCCATTGAGG GCTATGCAAT TGAACGCCAA TGGCCATGGC
    CTGGAGCCAG ATTACGCTGT TAGCACGAGG CAGCGCCAAC CACCGGCTCC GTACGGTGGC
    TCTCTGGTAA AGATTGCTGG AGCACCAATG CCGCCACGGC TCCCCGGTCA AGGATATCCC
    ATTCAAGCGC CGACAGCGCC AGCATACCAT CACCAGTCGG CCCAAAACTT AAGCAATATG
    TCCAGAAACA CATTGCTCGC TTTGAGTGCC ACCCCCAAAC CAAAATACGC GGACGGATGG
    GTGCAGGTGC AGCAGCGGAA GAGTTATGAC AGCCAACAGA CCAGCGATGC GGCTTGGCTG
    GCCGCTCAGC AGCAAAAGCG CAAATCCATG CCTGACTACG GCGGAGCACT CTACAATAAC
    AACCACTGGC TACTGCAGGA GGCGGAGCAG CGACGCATCG AGCAACTTAA TCGGCGATCT
    ATACCAGCCA GCAAGTCGAT GGGCAAACCG CTGCCCGATT CCATCATACA AACTCTTACG
    GAGCGGGTCC AGAGCAAAGG AATCGGCGAT CGAAAGCGCT TCGATAGCAA CGGGAACTAT
    AGCCAGGTAA ATGGCAACAA CATCTACCAG CAGCAACAGC AGCAGAAGAA TGTGACGAAT
    GGCAGCAGCA TCAATGGCAA CAGCAGCCAG GGGCAGGAGA AGGTGCTCAG CGTTAGTGGC
    AAGAAGAAGT GCTCCCATTG CGGCGACGAA TTAGATCTGG GCTGCACTCA CGGCAATTGT
    CGGCAGGTGC ACAAAGCGCT AAAATATATT GTTTGGGTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa08058
    Clone ID Related Accession (Same CDS sequence) NM_001144533.3
    Accession Version NM_001144533.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4602bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product smallish, isoform M
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001144533.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGCCA CTGATCAGGA GGTCATGCTG GAGGGCAGGG AAGACCTCAT GTCCACATCG 
    GCGACATCGT CCACAAATGG CGGGGACACT AATGGTTGCG GCAAAAAATT GTCCGTAACC
    TCTCCGGATC CCACACCGTA CGTGACCAAG CAGCGAGTGA CGCGTCCAAC GCCTCCCGCT
    CCTTCAAAGA ATCCGATGCA ATTCGTCCAG ATCAAGCCCT GCAACCTCTA CCAGACCGCG
    CAGGAGCAGC TGAAGAAGGC CGAGGAGGTC AAGAAGCTGA AGGAGGTGAA GAAGGAGGAG
    CCAGAGGAGT GGCAGAACAA CCTTGACAAT TGGAAATCAT CGAGACGAAA ACGTGTCGAA
    CACATCATCG ATCGAGCGGT GGAGACCAAG AAACTCGAGC TGGAGGAGCA TGATCGGTTG
    CGCCGAAAGT CGAAAACATT CACCGAAATG ATGGAGGAGA GGGCTGAAAG GGGCGGACCT
    CGGGGGCGTG CCAAGCTGGC TTCCCTGGCC GTATACAACG AGGATGAGAC GAACGACCTG
    AGTGATTTGG GGATCGGAAC CAGCAGTGCC AGCGGAAAGA GCAGCCTGTC CGGGGACTAT
    GACAATAACA GTGTTATGAG CGACCATGCG GCGGAGCTGG ATAAGGCGAT TGGAGCAGCT
    AGCTCTGTAG CTGCTGGAGT AACCGCCCAA AACGTGGATG AGCAGCTGAA TCACATAAAC
    CGCAACGGTA GTAATGGGAA CCACGGGAAC GGAGCGGCTG TTGGCCAGAC AGGACCATCA
    AATTCCTCCA AGTCAGCAGG GCGGCAATAC ATCTCCTCGC CAGGATACGA TACGTCCTCC
    AGTAACGCGC AAGCCAGTTC TCCGGATCTC TGTGAATACA CCTACGAAGG AGCCATCCAG
    GACTACAAGC AAAGAGTTCA ACGGGCCAGC AGCAATGGCA ATGGAAATGC CAACGGCAAG
    TTGATTGGAG AACATATTGC TTATCCCACG AGGCGAGGCT CTAAAATTGA GGATCGATTG
    AGTGGTTTTG AGGTGACATC GCCAAGCGAC ACGCAGGAGG GTGTAGAGAA GCAAAAGGTG
    GACGTGCCCA AGGTTGACAT ATCCAAACGC AAGGAGATCT TCGAGCAGGC CAAGGCGGAA
    GTCTCAAACG GTGGTGCTCC AGCTGCCCCA AAACTCGTAT TTCGTGATGG TCTTACCAAT
    GGAAATTCAG CTACCACTGC TCCCAAATCG GAGGTGAGGC GTCTTTCTGG TGACATCTCC
    AGCATTCGAG ATCGCATGCA GAGTTTGGAA CAGCAGCGGA AGGCTTTCAG TTCCAGCAAG
    AGTGTGGATG TGCCGGTGCC GCCCCTTAAA CAGCGTCTGA ATAGTCTGCA GCAGTCCGTT
    GCCAAAGAGG AGCAGAAGAA GCCACCGTTG GTGGCGCTCA TCGATGCCAG ACAACTGGAG
    ATCATGAGGG GAGAAGAGGA ACGTATGCGC CAGCAGCAAC AAAAAGAGAA GCATTCACCA
    AGCCAAACTA CATTGTGTGC ACCTAAGCCA GCTCCTCCTG CCCTTATTAT TGAGGAACCG
    CCGGTGGCAG ACACCAACGA CGACAGCGGC ATTCAGGAGG ACACCGCTGA TGAACTGCAG
    CAGCAGCAGC AACTCAACGC AGCCATCGCC GCCTTGGCGC TCGAGGAGCG CCAGTTGGAG
    GAAGCAGCCA ATGCGGTCAA CCAAATTGAA GCGGAGTTCG ATGAGCTGAC TGATCTGCAT
    CCCTCGCCAT TGCCACCATC TCCAGCTCTT CCGGCGGTTC AACAAAGCCT CCAACAACCG
    ACAGTCGCTC AGGTTCCAGC ACAAGCGGTC AATCAGGCGG CTGCTCCTCC ATTGCGGGAC
    ATGGAATTTA GTATCAACGA AGCTCTTGAA TTGGCGCTGC AAGCTATTGA TCGCGAGGCG
    ACAACCAAGC CAATGGACAA GGCACAACAT CCACAGAAGC GGGAACAGAA GGAGGAGCTG
    GGAGGTAAGC CGGAGGAGAA GGAGCCAATT TGCGAGGAGC AGCACGACGA AGAGTCCAAC
    GAGCAGGGGG AAACACACAA CGAGGGTGGG GAAATGCCAA ATAAAACCGA TAAGCAAATG
    CAGGGGGGAC GAGAGCCCAT CTATGAGAAT GTCAGCTCGA CTATATCTAT GCATGAAGTA
    GATAATGAAC AGATAGCAAC TATGACGATA ACCACACAGA CCCAAGCGAC GCGAATAAGT
    CACAGGAGAA GCATTCCAAG CCAGCAAACC AGCAACAGTA ACGAGAATAA TCAAAGCACT
    AATCAAACCA GCAACAGCAG GAATCAAAAA GAGAGCAGCA CAGCAGCAAC ATCGGCAGCG
    AACCCCGTGC CACCAACAAC ACCGGGGGAA CAAGCAGAGC CCTACTACCA AGTGCCGAAG
    GCCACGGAGC CCTACTACGA TGCCCCCAAG CATTTAAGGC CGGTGCCGGT ATACGAAAAT
    GTCGAGATCT TTTACTCGGG CCTGGAGATT AGTCAGGGAT CTGTGGGAGC GCCAGTAGGG
    CTGATGGAGC CACCCAAGGA AAAGCCACCA CCACCGCCCA CCGAGAGTCC GATTCCGCTG
    GACGAGGGCC ATGACGACGA GCTGGATGGA CTGGGCAACA GTCTGGGCCA CAACACAGAC
    ACCTGGTCAT CGGACAACAC CTACGAGACC ATATCGAACG GCACTCGCCG GCACCTTCAG
    GGACAACCGG AACCCGCCCA ACCCTCGCCG CCCATCAAGA GGATGAACTC GACCAAACGG
    ATCAAGAAGG AGTTGCGCAA CAAGCGCTCC AGCTTCCTGG GCATCGAAAA CGACGGCGAT
    CTTGACGACA TGGAGACCTA TCTCGAGCTA ACAGTGGCCC CGCCTCCGGA TATGGCGCAG
    CTCTTGCAGG AGGAGCGGCG ACTGGAGAAG CAGCTGTACA TCAAGGCGGG ACTCTGCGAC
    AGTTCCGACA CAGGTGAAAG CCGCGATTCT GGAGTTTCTG AAAACCACTC TCGCCAGAGC
    AGTGAGCACT ACACGAACTC CTCTGAGGAG AACGATACAC AGTCAGAGGC CACACCGCCG
    CCATTGCCTC CACCACCATC GACCGCCCAA GTGGGTGAAG TCATATACCA AAATGAGAGC
    CTCCTGGCTG CCCAGACGCC GCTTCTCCAG ACGGTAAAAG GAAACTCCGC CGAGTCCTGG
    ACCGAATCAG CAACGGCCGC AGCGGCGGCC ACGCAATCAT TGAGCGAAGC CACAGCAACG
    GCCAATGCCA AGATGCAGTC CATCGAGGAT AAGATCCGGG AGCAGGGAGA GATGTTGCGG
    GTGGAACGCG AGCTACTGCA CTTCTCGCAA GAGGAACTGA AACGGCAACG TGAAAATCTT
    GTGCTTCGAG AAAATATTGC TCGACGAGAG TTGCAACACG GAGCCAAGAT GCTGATGTCG
    AACAACCGGC GCTCACTGCA GGATCTGCAC CACGGTCTAG GAATCGGAAA CGGAATGCTC
    AGTGCCTTCC AGCCACCGCA ACACCACCAG GTTCAAATGC CACCACCGCA TCCGCAACAG
    ATTTACGCCA ATGTGCCGCA GCAGCAGCAA CAGATGGTTC TGCATGCATA TCACCAGATG
    GACACGGACT ACCGCAAGTC CATGTCGGAT CTAAACGAGT TCTCCAACTG CCTGATACTG
    CCGCCAACGC CGCCCACAAA GCCATTGAGG GCTATGCAAT TGAACGCCAA TGGCCATGGC
    CTGGAGCCAG ATTACGCTGT TAGCACGAGG CAGCGCCAAC CACCGGCTCC GTACGGTGGC
    TCTCTGGTAA AGATTGCTGG AGCACCAATG CCGCCACGGC TCCCCGGTCA AGGATATCCC
    ATTCAAGCGC CGACAGCGCC AGCATACCAT CACCAGTCGG CCCAAAACTT AAGCAATATG
    TCCAGAAACA CATTGCTCGC TTTGAGTGCC ACCCCCAAAC CAAAATACGC GGACGGATGG
    GTGCAGGTGC AGCAGCGGAA GAGTTATGAC AGCCAACAGA CCAGCGATGC GGCTTGGCTG
    GCCGCTCAGC AGCAAAAGCG CAAATCCATG CCTGACTACG GCGGAGCACT CTACAATAAC
    AACCACTGGC TACTGCAGGA GGCGGAGCAG CGACGCATCG AGCAACTTAA TCGGCGATCT
    ATACCAGCCA GCAAGTCGAT GGGCAAACCG CTGCCCGATT CCATCATACA AACTCTTACG
    GAGCGGGTCC AGAGCAAAGG AATCGGCGAT CGAAAGCGCT TCGATAGCAA CGGGAACTAT
    AGCCAGGTAA ATGGCAACAA CATCTACCAG CAGCAACAGC AGCAGAAGAA TGTGACGAAT
    GGCAGCAGCA TCAATGGCAA CAGCAGCCAG GGGCAGGAGA AGGTGCTCAG CGTTAGTGGC
    AAGAAGAAGT GCTCCCATTG CGGCGACGAA TTAGGTCGCG GCGCTGCCAT GATCATCGAA
    TCGCTTCTGC TGTTCTACCA CATCAACTGC TTCAAGTGTT GCGTCTGCCA CGTCCAGCTG
    GGAGATGGCC TCAATGGAAC CGATGTCCGG GTGCGAAACC ACAAGCTGCA TTGCCAAAAC
    TGCTACTCCA GCGATGACGG CATTAAGTTC AGCTGCGTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138005.2
    CDS614..5215
    Translation

    Target ORF information:

    RefSeq Version NM_001144533.3
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster smallish (smash), transcript variant M, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144533.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGAGGCCA CTGATCAGGA GGTCATGCTG GAGGGCAGGG AAGACCTCAT GTCCACATCG 
    GCGACATCGT CCACAAATGG CGGGGACACT AATGGTTGCG GCAAAAAATT GTCCGTAACC
    TCTCCGGATC CCACACCGTA CGTGACCAAG CAGCGAGTGA CGCGTCCAAC GCCTCCCGCT
    CCTTCAAAGA ATCCGATGCA ATTCGTCCAG ATCAAGCCCT GCAACCTCTA CCAGACCGCG
    CAGGAGCAGC TGAAGAAGGC CGAGGAGGTC AAGAAGCTGA AGGAGGTGAA GAAGGAGGAG
    CCAGAGGAGT GGCAGAACAA CCTTGACAAT TGGAAATCAT CGAGACGAAA ACGTGTCGAA
    CACATCATCG ATCGAGCGGT GGAGACCAAG AAACTCGAGC TGGAGGAGCA TGATCGGTTG
    CGCCGAAAGT CGAAAACATT CACCGAAATG ATGGAGGAGA GGGCTGAAAG GGGCGGACCT
    CGGGGGCGTG CCAAGCTGGC TTCCCTGGCC GTATACAACG AGGATGAGAC GAACGACCTG
    AGTGATTTGG GGATCGGAAC CAGCAGTGCC AGCGGAAAGA GCAGCCTGTC CGGGGACTAT
    GACAATAACA GTGTTATGAG CGACCATGCG GCGGAGCTGG ATAAGGCGAT TGGAGCAGCT
    AGCTCTGTAG CTGCTGGAGT AACCGCCCAA AACGTGGATG AGCAGCTGAA TCACATAAAC
    CGCAACGGTA GTAATGGGAA CCACGGGAAC GGAGCGGCTG TTGGCCAGAC AGGACCATCA
    AATTCCTCCA AGTCAGCAGG GCGGCAATAC ATCTCCTCGC CAGGATACGA TACGTCCTCC
    AGTAACGCGC AAGCCAGTTC TCCGGATCTC TGTGAATACA CCTACGAAGG AGCCATCCAG
    GACTACAAGC AAAGAGTTCA ACGGGCCAGC AGCAATGGCA ATGGAAATGC CAACGGCAAG
    TTGATTGGAG AACATATTGC TTATCCCACG AGGCGAGGCT CTAAAATTGA GGATCGATTG
    AGTGGTTTTG AGGTGACATC GCCAAGCGAC ACGCAGGAGG GTGTAGAGAA GCAAAAGGTG
    GACGTGCCCA AGGTTGACAT ATCCAAACGC AAGGAGATCT TCGAGCAGGC CAAGGCGGAA
    GTCTCAAACG GTGGTGCTCC AGCTGCCCCA AAACTCGTAT TTCGTGATGG TCTTACCAAT
    GGAAATTCAG CTACCACTGC TCCCAAATCG GAGGTGAGGC GTCTTTCTGG TGACATCTCC
    AGCATTCGAG ATCGCATGCA GAGTTTGGAA CAGCAGCGGA AGGCTTTCAG TTCCAGCAAG
    AGTGTGGATG TGCCGGTGCC GCCCCTTAAA CAGCGTCTGA ATAGTCTGCA GCAGTCCGTT
    GCCAAAGAGG AGCAGAAGAA GCCACCGTTG GTGGCGCTCA TCGATGCCAG ACAACTGGAG
    ATCATGAGGG GAGAAGAGGA ACGTATGCGC CAGCAGCAAC AAAAAGAGAA GCATTCACCA
    AGCCAAACTA CATTGTGTGC ACCTAAGCCA GCTCCTCCTG CCCTTATTAT TGAGGAACCG
    CCGGTGGCAG ACACCAACGA CGACAGCGGC ATTCAGGAGG ACACCGCTGA TGAACTGCAG
    CAGCAGCAGC AACTCAACGC AGCCATCGCC GCCTTGGCGC TCGAGGAGCG CCAGTTGGAG
    GAAGCAGCCA ATGCGGTCAA CCAAATTGAA GCGGAGTTCG ATGAGCTGAC TGATCTGCAT
    CCCTCGCCAT TGCCACCATC TCCAGCTCTT CCGGCGGTTC AACAAAGCCT CCAACAACCG
    ACAGTCGCTC AGGTTCCAGC ACAAGCGGTC AATCAGGCGG CTGCTCCTCC ATTGCGGGAC
    ATGGAATTTA GTATCAACGA AGCTCTTGAA TTGGCGCTGC AAGCTATTGA TCGCGAGGCG
    ACAACCAAGC CAATGGACAA GGCACAACAT CCACAGAAGC GGGAACAGAA GGAGGAGCTG
    GGAGGTAAGC CGGAGGAGAA GGAGCCAATT TGCGAGGAGC AGCACGACGA AGAGTCCAAC
    GAGCAGGGGG AAACACACAA CGAGGGTGGG GAAATGCCAA ATAAAACCGA TAAGCAAATG
    CAGGGGGGAC GAGAGCCCAT CTATGAGAAT GTCAGCTCGA CTATATCTAT GCATGAAGTA
    GATAATGAAC AGATAGCAAC TATGACGATA ACCACACAGA CCCAAGCGAC GCGAATAAGT
    CACAGGAGAA GCATTCCAAG CCAGCAAACC AGCAACAGTA ACGAGAATAA TCAAAGCACT
    AATCAAACCA GCAACAGCAG GAATCAAAAA GAGAGCAGCA CAGCAGCAAC ATCGGCAGCG
    AACCCCGTGC CACCAACAAC ACCGGGGGAA CAAGCAGAGC CCTACTACCA AGTGCCGAAG
    GCCACGGAGC CCTACTACGA TGCCCCCAAG CATTTAAGGC CGGTGCCGGT ATACGAAAAT
    GTCGAGATCT TTTACTCGGG CCTGGAGATT AGTCAGGGAT CTGTGGGAGC GCCAGTAGGG
    CTGATGGAGC CACCCAAGGA AAAGCCACCA CCACCGCCCA CCGAGAGTCC GATTCCGCTG
    GACGAGGGCC ATGACGACGA GCTGGATGGA CTGGGCAACA GTCTGGGCCA CAACACAGAC
    ACCTGGTCAT CGGACAACAC CTACGAGACC ATATCGAACG GCACTCGCCG GCACCTTCAG
    GGACAACCGG AACCCGCCCA ACCCTCGCCG CCCATCAAGA GGATGAACTC GACCAAACGG
    ATCAAGAAGG AGTTGCGCAA CAAGCGCTCC AGCTTCCTGG GCATCGAAAA CGACGGCGAT
    CTTGACGACA TGGAGACCTA TCTCGAGCTA ACAGTGGCCC CGCCTCCGGA TATGGCGCAG
    CTCTTGCAGG AGGAGCGGCG ACTGGAGAAG CAGCTGTACA TCAAGGCGGG ACTCTGCGAC
    AGTTCCGACA CAGGTGAAAG CCGCGATTCT GGAGTTTCTG AAAACCACTC TCGCCAGAGC
    AGTGAGCACT ACACGAACTC CTCTGAGGAG AACGATACAC AGTCAGAGGC CACACCGCCG
    CCATTGCCTC CACCACCATC GACCGCCCAA GTGGGTGAAG TCATATACCA AAATGAGAGC
    CTCCTGGCTG CCCAGACGCC GCTTCTCCAG ACGGTAAAAG GAAACTCCGC CGAGTCCTGG
    ACCGAATCAG CAACGGCCGC AGCGGCGGCC ACGCAATCAT TGAGCGAAGC CACAGCAACG
    GCCAATGCCA AGATGCAGTC CATCGAGGAT AAGATCCGGG AGCAGGGAGA GATGTTGCGG
    GTGGAACGCG AGCTACTGCA CTTCTCGCAA GAGGAACTGA AACGGCAACG TGAAAATCTT
    GTGCTTCGAG AAAATATTGC TCGACGAGAG TTGCAACACG GAGCCAAGAT GCTGATGTCG
    AACAACCGGC GCTCACTGCA GGATCTGCAC CACGGTCTAG GAATCGGAAA CGGAATGCTC
    AGTGCCTTCC AGCCACCGCA ACACCACCAG GTTCAAATGC CACCACCGCA TCCGCAACAG
    ATTTACGCCA ATGTGCCGCA GCAGCAGCAA CAGATGGTTC TGCATGCATA TCACCAGATG
    GACACGGACT ACCGCAAGTC CATGTCGGAT CTAAACGAGT TCTCCAACTG CCTGATACTG
    CCGCCAACGC CGCCCACAAA GCCATTGAGG GCTATGCAAT TGAACGCCAA TGGCCATGGC
    CTGGAGCCAG ATTACGCTGT TAGCACGAGG CAGCGCCAAC CACCGGCTCC GTACGGTGGC
    TCTCTGGTAA AGATTGCTGG AGCACCAATG CCGCCACGGC TCCCCGGTCA AGGATATCCC
    ATTCAAGCGC CGACAGCGCC AGCATACCAT CACCAGTCGG CCCAAAACTT AAGCAATATG
    TCCAGAAACA CATTGCTCGC TTTGAGTGCC ACCCCCAAAC CAAAATACGC GGACGGATGG
    GTGCAGGTGC AGCAGCGGAA GAGTTATGAC AGCCAACAGA CCAGCGATGC GGCTTGGCTG
    GCCGCTCAGC AGCAAAAGCG CAAATCCATG CCTGACTACG GCGGAGCACT CTACAATAAC
    AACCACTGGC TACTGCAGGA GGCGGAGCAG CGACGCATCG AGCAACTTAA TCGGCGATCT
    ATACCAGCCA GCAAGTCGAT GGGCAAACCG CTGCCCGATT CCATCATACA AACTCTTACG
    GAGCGGGTCC AGAGCAAAGG AATCGGCGAT CGAAAGCGCT TCGATAGCAA CGGGAACTAT
    AGCCAGGTAA ATGGCAACAA CATCTACCAG CAGCAACAGC AGCAGAAGAA TGTGACGAAT
    GGCAGCAGCA TCAATGGCAA CAGCAGCCAG GGGCAGGAGA AGGTGCTCAG CGTTAGTGGC
    AAGAAGAAGT GCTCCCATTG CGGCGACGAA TTAGGTCGCG GCGCTGCCAT GATCATCGAA
    TCGCTTCTGC TGTTCTACCA CATCAACTGC TTCAAGTGTT GCGTCTGCCA CGTCCAGCTG
    GGAGATGGCC TCAATGGAAC CGATGTCCGG GTGCGAAACC ACAAGCTGCA TTGCCAAAAC
    TGCTACTCCA GCGATGACGG CATTAAGTTC AGCTGCGTCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.