Ten-m cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ten-m
Entrez Gene ID	40464
Full Name	Tenascin major
Synonyms	2017,5301,BcDNA:AT25108,CG11452,CG5723,CT16449,Dmel\CG5723,Odz,Ten-mc,Ten79E,Ten[m],l(3)00844,l(3)05301,l(3)05309,l(3)rJ307,l(3)rL201,l(3)rP126,odd(Oz),odz,odz/ten-m,ten(m),ten-m,ten-m/odz,ten[m]
General protein information	Preferred Names Tenascin major Names CG5723 gene product from transcript CG5723-RB CG5723-PB CG5723-PD CG5723-PE Ten-m-PB Ten-m-PD Ten-m-PE odd Oz odd-Oz tenascin major tenascin-like molecule major tenascin-major teneurin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 79D4-79E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001275282.1	NP_001262211.1	tenascin major, isoform E
NM_001104191.3	NP_001097661.2	tenascin major, isoform D
NM_079491.3	NP_524215.2	tenascin major, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	TENM2	NP_001116151.1
Rattus norvegicus (Norway rat)	Tenm2	NP_064473.1
Drosophila melanogaster (fruit fly)	Ten-m	NP_524215.2
Xenopus tropicalis (tropical clawed frog)	tenm2	XP_002934407.2
Bos taurus (cattle)	TENM2	XP_002689409.2
Mus musculus (house mouse)	Tenm2	NP_035986.3
Caenorhabditis elegans (roundworm)	ten-1	NP_001022723.2
	ODZ2	XP_001090578.2
Danio rerio (zebrafish)	odz2	XP_691651.5
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011034	XP_308102.4
Gallus gallus (chicken)	ODZ2	NP_989428.2
Canis lupus familiaris (dog)	TENM2	XP_546253.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ten-m/Odz may have had an ancient role in cell-cell communication, parallel to the Notch and wnt pathways. The Ten-m protein binds to the membrane with properties which resemble other membrane-based biochemical oscillators. The oscillatory properties of Ten-m may play a seminal role in arthropod segmentation.
Title: Ancient role of

Ten-m acts in projection neurons autonomously to regulate acetylcholine receptor cluster number and transsynaptically to regulate olfactory receptor neurons active zone number.
Title: Synaptic organization of the Drosophila antennal lobe and its regulation by the Teneurins.

study identifies the Teneurins as a key bi-directional trans-synaptic signal involved in general synapse organization, and demonstrates that proteins such as these can also regulate target selection
Title: Trans-synaptic Teneurin signalling in neuromuscular synapse organization and target choice.

identification of two evolutionarily conserved, epidermal growth factor (EGF)-repeat containing transmembrane Teneurin proteins, Ten-m and Ten-a, as synaptic-partner-matching molecules between projection neuron dendrites and olfactory receptor neuron axons
Title: Teneurins instruct synaptic partner matching in an olfactory map.

Data indicate that during embryonic development, the expression of Filamin and Ten-m partially overlap in ectodermal cells.
Title: Drosophila Ten-m and filamin affect motor neuron growth cone guidance.

The following Ten-m gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ten-m cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19267	NM_079491.3 Latest version!	Drosophila melanogaster tenascin major, transcript variant B (Ten-m), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa19268	NM_001104191.3 Latest version!	Drosophila melanogaster tenascin major, transcript variant D (Ten-m), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa20341	NM_001275282.1 Latest version!	Drosophila melanogaster tenascin major, transcript variant E (Ten-m), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ten-m ( NM_079491.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524215.2 Drosophila melanogaster tenascin major, transcript variant B (Ten-m), mRNA.

Price & Availability↑

CloneID	OFa19267
Clone ID Related Accession (Same CDS sequence)	NM_079491.3
Accession Version	NM_079491.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tenascin major, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079491.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCCCT ACGAGTATGA GTCGACTCTA GACTGCCGCG ACGTGGGCGG CGGCCCCACT 
CCGGCTCACG CCCACCCACA CGCCCAAGGA CGTACGCTCC CCATGAGCGG CCACGGGCGG 
CCGACGACGG ACCTCGGTCC GGTCCACGGC AGCCAGACGC TCCAGCACCA AAACCAACAG 
AACCTGCAGG CCGCCCAAGC CGCCGCCCAG TCGTCCCACT ACGACTACGA GTACCAGCAC 
TTGGCCCACC GTCCGCCGGA CACGGCCAAC AACACAGCGC AGCGGACACA CGGAAGACAA 
GGCTTCCTCC TGGAAGGAGT CACGCCAACG GCACCACCGG ATGTTCCGCC GCGTAATCCG 
ACAATGAGTC GCATGCAAAA TGGTCGTTTA ACCGTTAACA ATCCCAATGA CGCTGACTTT 
GAGCCATCTT GTTTGGTGCG AACGCCATCG GGCAACGTTT ACATTCCTAG TGGAAATCTA 
AACATCAACA AGGGATCACC CATCGACTTC AAGAGCGGCT CGGCCTGCTC CACACCCACG 
AAGGATACGC TGAAGGGCTA CGAGCGGAGC ACGCAGGGCT GCATGGGTCC TGTGCTGCCG 
CAGCGCAGCG TCATGAACGG ACTGCCCGCA CACCACTACT CGGCGCCGAT GAACTTCCGC 
AAGGACCTGG TCGCGCGCTG CTCCTCGCCG TGGTTCGGTA TAGGATCCAT CTCGGTGCTC 
TTCGCCTTCG TGGTCATGCT TATCCTGCTA ACCACCACCG GCGTTATAAA ATGGAATCAG 
TCACCACCCT GCTCCGTTCT AGTCGGCAAC GAGGCCTCTG AGGTCACGGC TGCCAAGAGC 
ACGAACACGG ACCTCTCCAA GCTGCACAAT TCATCGGTGC GCGCGAAGAA CGGACAAGGA 
ATCGGACTGG CCCAGGGACA ATCGGGACTC GGAGCAGCAG GAGTGGGATC CGGCGGTGGA 
TCTTCCGCGG CCACTGTGAC GACGGCCACC TCGAACAGCG GCACCGCCCA AGGACTGCAG 
TCGACGTCGG CCTCCGCGGA GGCCACGTCC TCGGCGGCCA CGTCTTCCTC CCAATCCTCT 
CTAACGCCTT CGCTGTCCTC GTCCCTGGCG AATGCCAATA ATGGAGGAGC CCGAACATTC 
CCTGCACGCA GTTTCCCACC GGACGGCACT ACCTTTGGCC AGATTACTCT CGGCCAGAAG 
CTGACCAAGG AGATCCAGCC GTACAGCTAC TGGAACATGC AGTTTTACCA GTCGGAACCT 
GCCTACGTTA AGTTCGACTA CACGATTCCG AGGGGCGCCT CCATCGGTGT ATACGGCCGA 
CGCAACGCCC TACCCACCCA CACGCAGTAC CACTTTAAGG AAGTGCTCAG TGGATTTAGT 
GCCAGCACAC GAACGGCCCG GGCCGCTCAC CTGTCGATAA CTCGGGAGGT GACACGCTAT 
ATGGAGCCGG GGCACTGGTT CGTCTCGCTT TACAACGACG ACGGGGACGT GCAGGAGCTG 
ACCTTCTACG CGGCCGTAGC CGAGGACATG ACCCAAAACT GTCCCAACGG CTGCTCCGGC 
AACGGTCAGT GCCTGCTGGG TCACTGCCAG TGCAACCCCG GCTTCGGGGG CGACGACTGC 
AGCGAGAGCG TGTGCCCCGT ACTGTGCTCC CAACATGGCG AGTACACCAA CGGAGAGTGC 
ATCTGTAACC CGGGCTGGAA GGGCAAGGAG TGCTCTCTGC GCCACGACGA GTGCGAGGTG 
GCCGACTGCA GTGGGCACGG CCACTGTGTC AGTGGAAAAT GCCAATGCAT GCGCGGCTAC 
AAAGGCAAAT TCTGCGAAGA AGTTGACTGC CCACATCCGA ACTGCTCAGG CCACGGATTC 
TGCGCCGATG GCACTTGCAT ATGCAAGAAG GGCTGGAAGG GACCTGACTG CGCTACCATG 
GACCAGGATG CGCTGCAGTG CCTCCCCGAC TGTTCCGGCC ACGGCACCTT CGACCTGGAC 
ACACAGACCT GTACCTGCGA GGCAAAGTGG AGTGGGGACG ATTGTTCCAA GGAGCTGTGC 
GACTTGGACT GCGGTCAGCA CGGACGTTGT GAAGGTGACG CTTGTGCATG CGACCCGGAG 
TGGGGTGGCG AGTATTGCAA CACCCGGTTG TGCGACGTCC GCTGTAACGA GCATGGCCAG 
TGCAAGAATG GTACTTGTCT GTGCGTTACT GGATGGAACG GAAAGCACTG CACCATCGAG 
GGCTGTCCCA ATAGCTGCGC CGGGCATGGC CAGTGCCGTG TAAGTGGCGA GGGTCAATGG 
GAGTGTCGCT GCTACGAGGG CTGGGACGGG CCGGACTGTG GGATTGCACT GGAGTTGAAC 
TGTGGCGACA GCAAGGACAA CGACAAGGAT GGCTTGGTTG ATTGCGAGGA TCCTGAGTGC 
TGTGCCAGCC ACGTATGCAA GACCTCCCAA CTATGTGTTT CAGCTCCGAA GCCCATTGAT 
GTGCTGCTCA GAAAACAGCC GCCAGCTATT ACGGCCTCTT TCTTTGAGCG GATGAAGTTC 
CTCATCGACG AGAGCAGCCT GCAGAACTAC GCCAAACTGG AAACCTTTAA CGAAAGTCGA 
TCAGCGGTGA TCAGAGGCCG CGTTGTTACA TCTCTGGGCA TGGGACTGGT GGGTGTGCGA 
GTGTCCACCA CCACGCTCCT AGAGGGCTTC ACCCTGACCC GAGATGATGG ATGGTTCGAC 
CTAATGGTTA ACGGTGGTGG AGCCGTAACG CTGCAGTTCG GACGCGCACC TTTCCGGCCG 
CAGTCGCGCA TCGTGCAAGT TCCATGGAAC GAGGTCGTGA TTATTGACTT GGTCGTCATG 
AGTATGTCCG AGGAAAAGGG ATTGGCCGTA ACTACGACAC ACACATGCTT TGCACACGAC 
TACGACCTGA TGAAGCCCGT GGTGCTGGCT TCGTGGAAGC ACGGGTTTCA GGGAGCCTGT 
CCCGATCGCA GCGCCATTCT TGCTGAATCT CAAGTGATTC AGGAGTCGTT GCAGATCCCG 
GGTACAGGCC TCAACCTGGT TTACCACTCA TCGCGTGCTG CCGGCTACCT TTCCACTATT 
AAGCTGCAGT TAACTCCGGA TGTGATTCCG ACATCGCTAC ATCTTATCCA TCTCCGCATA 
ACAATCGAGG GAATACTCTT TGAACGAATA TTCGAAGCAG ATCCGGGAAT TAAGTTCACG 
TACGCCTGGA ACCGACTCAA CATCTACCGG CAACGAGTCT ACGGTGTAAC CACGGCCGTG 
GTGAAGGTTG GCTACCAGTA CACCGACTGC ACTGACATTG TGTGGGACAT TCAAACAACT 
AAGCTAAGCG GTCACGACAT GTCGATCTCA GAAGTGGGTG GGTGGAACCT GGACATCCAT 
CACCGCTACA ACTTCCACGA GGGAATTCTG CAAAAGGGCG ACGGCTCAAA CATATATCTG 
CGCAATAAGC CGCGAATCAT CCTGACCACA ATGGGAGATG GCCACCAGCG GCCTCTGGAG 
TGTCCCGATT GCGACGGCCA GGCGACAAAG CAGCGACTTC TGGCACCCGT CGCACTGGCC 
GCCGCTCCTG ACGGCAGTCT TTTCGTCGGT GATTTTAATT ACATCCGCCG CATCATGACC 
GACGGCAGCA TCCGCACTGT GGTCAAGCTG AACGCGACTC GCGTCTCGTA CCGCTACCAC 
ATGGCCCTCA GCCCGCTCGA CGGAACTCTT TATGTTTCGG ATCCAGAATC ACACCAAATC 
ATTCGGGTAC GCGACACCAA CGACTACTCG CAACCGGAAC TAAACTGGGA AGCGGTTGTG 
GGCTCCGGGG AGCGCTGTCT TCCTGGTGAC GAAGCGCACT GCGGCGATGG GGCACTGGCA 
AAGGATGCCA AATTGGCTTA TCCGAAAGGC ATTGCGATTT CGAGCGACAA CATCTTGTAC 
TTTGCAGACG GAACTAATAT CCGTATGGTG GACCGGGATG GAATTGTAAG CACTCTGATC 
GGCAACCACA TGCACAAGTC CCACTGGAAG CCAATTCCCT GCGAGGGAAC CCTGAAGCTG 
GAGGAGATGC ATCTTCGCTG GCCCACTGAG CTAGCAGTCT CCCCGATGGA CAACACGCTG 
CACATAATCG ACGACCACAT GATCCTGCGG ATGACACCAG ACGGTCGTGT GCGCGTCATC 
TCCGGCCGTC CCCTACATTG CGCCACAGCC TCCACTGCCT ACGATACGGA TCTGGCTACC 
CACGCTACCC TGGTGATGCC GCAGTCTATC GCGTTCGGTC CACTCGGTGA GCTTTACGTG 
GCGGAAAGCG ACTCTCAACG AATAAACCGG GTGCGCGTGA TCGGCACGGA CGGAAGGATC 
GCTCCGTTTG CTGGTGCCGA ATCCAAGTGC AATTGCCTGG AGCGCGGTTG CGACTGCTTC 
GAAGCGGAAC ACTATCTGGC CACCAGTGCC AAGTTCAACA CGATCGCCGC CCTTGCTGTC 
ACACCCGATA GCCATGTACA CATCGCGGAC CAGGCCAACT ACCGTATCCG CTCCGTTATG 
TCGAGCATCC CAGAGGCAAG TCCGTCGCGC GAGTACGAGA TCTACGCACC TGACATGCAG 
GAGATATACA TTTTCAACCG ATTCGGACAG CACGTGTCCA CACGCAACAT CCTGACCGGA 
GAGACGACCT ATGTGTTTAC CTACAACGTA AACACCTCCA ACGGAAAACT GAGCACCGTA 
ACAGACGCGG CTGGCAACAA AGTGTTCCTG CTTCGAGATT ACACTTCTCA GGTAAATTCC 
ATCGAGAACA CGAAGGGTCA AAAGTGCCGC CTGCGCATGA CCAGGATGAA AATGCTGCAC 
GAGCTGAGCA CTCCAGACAA CTACAATGTG ACCTACGAAT ATCACGGCCC CACCGGTCTG 
CTACGAACCA AGCTGGACTC CACCGGACGA TCCTACGTTT ACAACTACGA TGAGTTTGGT 
CGCCTCACTT CTGCAGTGAC TCCCACCGGT CGTGTCATCG AGCTGAGCTT CGACCTGAGC 
GTGAAGGGAG CCCAGGTAAA GGTTTCGGAG AACGCCCAGA AGGAAATGTC ACTGCTGATC 
CAAGGTGCCA CCGTTATTGT GCGCAACGGA GCCGCCGAGT CGCGTACCAC CGTTGACATG 
GACGGCTCCA CCACAAGCAT CACTCCATGG GGCCACAACC TGCAGATGGA AGTGGCACCT 
TATACCATTC TGGCAGAACA AAGTCCACTG CTTGGCGAGA GCTACCCGGT CCCGGCTAAG 
CAACGCACTG AGATTGCTGG TGACTTGGCC AACAGGTTTG AGTGGCGCTA CTTTGTGCGC 
CGGCAGCAGC CGCTGCAAGC GGGAAAACAG AGCAAGGGAC CGCCACGTCC CGTAACCGAA 
GTTGGACGTA AGCTGCGTGT GAACGGAGAC AACGTGCTGA CTTTGGAGTA CGACCGCGAG 
ACCCAGTCGG TGGTCGTTAT GGTGGACGAC AAGCAGGAGC TGCTTAACGT GACCTACGAT 
CGCACTTCCC GCCCCATCAG CTTCCGACCG CAGTCCGGCG ACTACGCGGA CGTGGACTTG 
GAGTACGATC GGTTCGGACG TCTGGTCAGT TGGAAGTGGG GTGTCCTGCA AGAAGCCTAC 
TCTTTCGACC GCAATGGCCG TCTGAACGAG ATCAAGTACG GCGATGGCTC AACAATGGTC 
TACGCATTCA AGGACATGTT TGGCTCGTTA CCGCTGAAGG TGACTACGCC TAGACGTTCT 
GATTATCTCC TGCAGTACGA TGATGCAGGT GCACTCCAGA GCCTGACGAC ACCTCGTGGA 
CACATTCACG CATTCTCCCT GCAAACTTCT CTTGGCTTCT TTAAGTACCA ATACTATTCA 
CCAATCAACC GACACCCCTT CGAGATTCTA TATAACGACG AAGGACAGAT CTTGGCCAAA 
ATCCACCCCC ACCAATCTGG CAAGGTGGCA TTCGTACATG ATACTGCCGG ACGACTGGAG 
ACGATCCTCG CAGGATTGTC CAGCACCCAC TATACCTACC AGGATACAAC TAGCCTGGTA 
AAGTCCGTGG AGGTGCAGGA GCCGGGCTTC GAGTTGCGCC GAGAGTTTAA ATACCATGCA 
GGTATCCTAA AGGACGAGAA GTTGCGCTTC GGCTCCAAGA ACTCGCTGGC CTCGGCGCGC 
TACAAGTACG CCTACGACGG AAACGCCCGT CTCAGCGGCA TCGAGATGGC CATCGACGAT 
AAGGAATTAC CAACCACGCG GTACAAGTAC AGCCAAAACT TGGGACAACT GGAGGTCGTG 
CAGGACCTGA AGATCACGCG CAACGCTTTT AACCGAACAG TCATACAGGA CTCTGCCAAA 
CAATTCTTCG CCATTGTGGA CTACGACCAG CATGGACGGG TTAAGAGCGT GCTAATGAAC 
GTGAAGAACA TTGATGTGTT CCGCCTTGAG TTGGACTACG ATCTTCGCAA CCGCATCAAG 
TCGCAAAAGA CGACCTTCGG TAGATCGACA GCCTTTGATA AGATTAACTA CAATGCCGAC 
GGACATGTTG TTGAGGTCCT GGGCACCAAC AACTGGAAGT ATCTGTTCGA CGAGAACGGC 
AACACTGTTG GCGTGGTTGA CCAGGGTGAG AAGTTCAACC TGGGCTACGA CATCGGAGAC 
CGGGTGATCA AGGTAGGCGA TGTGGAGTTC AACAACTACG ATGCCCGCGG CTTCGTTGTG 
AAGCGCGGTG AGCAGAAGTA CCGCTACAAC AACCGCGGAC AACTGATTCA CTCGTTCGAA 
AGGGAACGTT TCCAGAGCTG GTACTACTAC GACGACCGCA GCCGCCTCGT GGCCTGGCAT 
GATAACAAAG GTAACACCAC GCAGTACTAC TACGCCAATC CTCGCACTCC TCATCTCGTG 
ACCCACGTTC ACTTCCCGAA GATCAGCCGC ACCATGAAGC TGTTCTACGA TGACCGCGAC 
ATGCTAATCG CTTTGGAGCA TGAGGACCAG CGCTACTACG TGGCCACCGA CCAGAACGGC 
TCGCCACTAG CCTTCTTTGA TCAGAATGGC AGCATTGTCA AGGAAATGAA GCGAACGCCC 
TTCGGCCGAA TCATTAAGGA CACTAAGCCG GAGTTCTTTG TGCCCATCGA CTTCCACGGC 
GGACTGATCG ACCCACACAC AAAGCTGGTG TACACCGAGC AGCGGCAGTA CGACCCGCAC 
GTTGGCCAAT GGATGACCCC GTTGTGGGAG ACGCTCGCCA CCGAGATGTC ACACCCGACG 
GATGTTTTTA TATACCGCTA CCACAACAAC GACCCCATCA ACCCGAACAA GCCCCAAAAC 
TATATGATCG ATCTGGATTC CTGGCTCCAG TTATTCGGCT ACGATTTGAA CAACATGCAA 
AGCAGCCGCT ACACCAAGCT GGCCCAGTAT ACGCCGCAGG CTTCCATCAA GTCAAACACG 
TTGGCCCCCG ACTTTGGCGT GATCTCCGGC CTGGAGTGCA TCGTGGAAAA AACAAGTGAG 
AAGTTCAGTG ATTTTGACTT TGTGCCCAAA CCGCTGCTGA AAATGGAGCC AAAGATGCGC 
AACTTGCTCC CGCGTGTCAG CTACCGGCGC GGTGTGTTCG GCGAAGGTGT ACTCCTCTCG 
AGAATCGGCG GACGGGCGTT AGTTAGCGTG GTCGACGGAT CAAACAGCGT GGTGCAGGAC 
GTAGTGAGCA GCGTGTTCAA CAACTCTTAC TTCCTTGACC TACACTTCAG CATTCACGAC 
CAAGACGTAT TTTACTTCGT AAAGGATAAT GTCCTGAAGC TGCGCGACGA CAACGAGGAG 
CTGCGTCGCC TTGGCGGCAT GTTCAACATA TCAACCCATG AAATCAGCGA CCACGGAGGC 
AGCGCCGCTA AGGAGCTGCG TCTTCACGGC CCAGACGCCG TTGTCATTAT CAAATATGGC 
GTTGATCCCG AGCAGGAGCG CCACCGCATC CTAAAGCATG CCCACAAGCG GGCAGTGGAG 
CGGGCTTGGG AGTTGGAGAA GCAGCTGGTG GCTGCCGGCT TCCAAGGCCG TGGCGACTGG 
ACCGAGGAGG AGAAAGAGGA GCTCGTCCAA CACGGTGACG TCGACGGCTG GAACGGAATC 
GATATCCACA GCATACACAA GTATCCGCAG CTGGCCGACG ACCCCGGTAA CGTGGCTTTC 
CAGCGGGACG CGAAGCGTAA GCGACGCAAG ACCGGCAGCA GCCATCGGAG TGCCTCCAAC 
CGCCGCCAGC TCAAGTTCGG CGAGCTGAGT GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524215.2
CDS	672..8867
Translation	MNPYEYESTLDCRDVGGGPTPAHAHPHAQGRTLPMSGHGRPTTDLGPVHGSQTLQHQNQQNLQAAQAAAQSSHYDYEYQHLAHRPPDTANNTAQRTHGRQGFLLEGVTPTAPPDVPPRNPTMSRMQNGRLTVNNPNDADFEPSCLVRTPSGNVYIPSGNLNINKGSPIDFKSGSACSTPTKDTLKGYERSTQGCMGPVLPQRSVMNGLPAHHYSAPMNFRKDLVARCSSPWFGIGSISVLFAFVVMLILLTTTGVIKWNQSPPCSVLVGNEASEVTAAKSTNTDLSKLHNSSVRAKNGQGIGLAQGQSGLGAAGVGSGGGSSAATVTTATSNSGTAQGLQSTSASAEATSSAATSSSQSSLTPSLSSSLANANNGGARTFPARSFPPDGTTFGQITLGQKLTKEIQPYSYWNMQFYQSEPAYVKFDYTIPRGASIGVYGRRNALPTHTQYHFKEVLSGFSASTRTARAAHLSITREVTRYMEPGHWFVSLYNDDGDVQELTFYAAVAEDMTQNCPNGCSGNGQCLLGHCQCNPGFGGDDCSESVCPVLCSQHGEYTNGECICNPGWKGKECSLRHDECEVADCSGHGHCVSGKCQCMRGYKGKFCEEVDCPHPNCSGHGFCADGTCICKKGWKGPDCATMDQDALQCLPDCSGHGTFDLDTQTCTCEAKWSGDDCSKELCDLDCGQHGRCEGDACACDPEWGGEYCNTRLCDVRCNEHGQCKNGTCLCVTGWNGKHCTIEGCPNSCAGHGQCRVSGEGQWECRCYEGWDGPDCGIALELNCGDSKDNDKDGLVDCEDPECCASHVCKTSQLCVSAPKPIDVLLRKQPPAITASFFERMKFLIDESSLQNYAKLETFNESRSAVIRGRVVTSLGMGLVGVRVSTTTLLEGFTLTRDDGWFDLMVNGGGAVTLQFGRAPFRPQSRIVQVPWNEVVIIDLVVMSMSEEKGLAVTTTHTCFAHDYDLMKPVVLASWKHGFQGACPDRSAILAESQVIQESLQIPGTGLNLVYHSSRAAGYLSTIKLQLTPDVIPTSLHLIHLRITIEGILFERIFEADPGIKFTYAWNRLNIYRQRVYGVTTAVVKVGYQYTDCTDIVWDIQTTKLSGHDMSISEVGGWNLDIHHRYNFHEGILQKGDGSNIYLRNKPRIILTTMGDGHQRPLECPDCDGQATKQRLLAPVALAAAPDGSLFVGDFNYIRRIMTDGSIRTVVKLNATRVSYRYHMALSPLDGTLYVSDPESHQIIRVRDTNDYSQPELNWEAVVGSGERCLPGDEAHCGDGALAKDAKLAYPKGIAISSDNILYFADGTNIRMVDRDGIVSTLIGNHMHKSHWKPIPCEGTLKLEEMHLRWPTELAVSPMDNTLHIIDDHMILRMTPDGRVRVISGRPLHCATASTAYDTDLATHATLVMPQSIAFGPLGELYVAESDSQRINRVRVIGTDGRIAPFAGAESKCNCLERGCDCFEAEHYLATSAKFNTIAALAVTPDSHVHIADQANYRIRSVMSSIPEASPSREYEIYAPDMQEIYIFNRFGQHVSTRNILTGETTYVFTYNVNTSNGKLSTVTDAAGNKVFLLRDYTSQVNSIENTKGQKCRLRMTRMKMLHELSTPDNYNVTYEYHGPTGLLRTKLDSTGRSYVYNYDEFGRLTSAVTPTGRVIELSFDLSVKGAQVKVSENAQKEMSLLIQGATVIVRNGAAESRTTVDMDGSTTSITPWGHNLQMEVAPYTILAEQSPLLGESYPVPAKQRTEIAGDLANRFEWRYFVRRQQPLQAGKQSKGPPRPVTEVGRKLRVNGDNVLTLEYDRETQSVVVMVDDKQELLNVTYDRTSRPISFRPQSGDYADVDLEYDRFGRLVSWKWGVLQEAYSFDRNGRLNEIKYGDGSTMVYAFKDMFGSLPLKVTTPRRSDYLLQYDDAGALQSLTTPRGHIHAFSLQTSLGFFKYQYYSPINRHPFEILYNDEGQILAKIHPHQSGKVAFVHDTAGRLETILAGLSSTHYTYQDTTSLVKSVEVQEPGFELRREFKYHAGILKDEKLRFGSKNSLASARYKYAYDGNARLSGIEMAIDDKELPTTRYKYSQNLGQLEVVQDLKITRNAFNRTVIQDSAKQFFAIVDYDQHGRVKSVLMNVKNIDVFRLELDYDLRNRIKSQKTTFGRSTAFDKINYNADGHVVEVLGTNNWKYLFDENGNTVGVVDQGEKFNLGYDIGDRVIKVGDVEFNNYDARGFVVKRGEQKYRYNNRGQLIHSFERERFQSWYYYDDRSRLVAWHDNKGNTTQYYYANPRTPHLVTHVHFPKISRTMKLFYDDRDMLIALEHEDQRYYVATDQNGSPLAFFDQNGSIVKEMKRTPFGRIIKDTKPEFFVPIDFHGGLIDPHTKLVYTEQRQYDPHVGQWMTPLWETLATEMSHPTDVFIYRYHNNDPINPNKPQNYMIDLDSWLQLFGYDLNNMQSSRYTKLAQYTPQASIKSNTLAPDFGVISGLECIVEKTSEKFSDFDFVPKPLLKMEPKMRNLLPRVSYRRGVFGEGVLLSRIGGRALVSVVDGSNSVVQDVVSSVFNNSYFLDLHFSIHDQDVFYFVKDNVLKLRDDNEELRRLGGMFNISTHEISDHGGSAAKELRLHGPDAVVIIKYGVDPEQERHRILKHAHKRAVERAWELEKQLVAAGFQGRGDWTEEEKEELVQHGDVDGWNGIDIHSIHKYPQLADDPGNVAFQRDAKRKRRKTGSSHRSASNRRQLKFGELSA

Target ORF information:

RefSeq Version	NM_079491.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tenascin major, transcript variant B (Ten-m), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079491.3

ORF Insert Sequence:

ATGAATCCCT ACGAGTATGA GTCGACTCTA GACTGCCGCG ACGTGGGCGG CGGCCCCACT 
CCGGCTCACG CCCACCCACA CGCCCAAGGA CGTACGCTCC CCATGAGCGG CCACGGGCGG 
CCGACGACGG ACCTCGGTCC GGTCCACGGC AGCCAGACGC TCCAGCACCA AAACCAACAG 
AACCTGCAGG CCGCCCAAGC CGCCGCCCAG TCGTCCCACT ACGACTACGA GTACCAGCAC 
TTGGCCCACC GTCCGCCGGA CACGGCCAAC AACACAGCGC AGCGGACACA CGGAAGACAA 
GGCTTCCTCC TGGAAGGAGT CACGCCAACG GCACCACCGG ATGTTCCGCC GCGTAATCCG 
ACAATGAGTC GCATGCAAAA TGGTCGTTTA ACCGTTAACA ATCCCAATGA CGCTGACTTT 
GAGCCATCTT GTTTGGTGCG AACGCCATCG GGCAACGTTT ACATTCCTAG TGGAAATCTA 
AACATCAACA AGGGATCACC CATCGACTTC AAGAGCGGCT CGGCCTGCTC CACACCCACG 
AAGGATACGC TGAAGGGCTA CGAGCGGAGC ACGCAGGGCT GCATGGGTCC TGTGCTGCCG 
CAGCGCAGCG TCATGAACGG ACTGCCCGCA CACCACTACT CGGCGCCGAT GAACTTCCGC 
AAGGACCTGG TCGCGCGCTG CTCCTCGCCG TGGTTCGGTA TAGGATCCAT CTCGGTGCTC 
TTCGCCTTCG TGGTCATGCT TATCCTGCTA ACCACCACCG GCGTTATAAA ATGGAATCAG 
TCACCACCCT GCTCCGTTCT AGTCGGCAAC GAGGCCTCTG AGGTCACGGC TGCCAAGAGC 
ACGAACACGG ACCTCTCCAA GCTGCACAAT TCATCGGTGC GCGCGAAGAA CGGACAAGGA 
ATCGGACTGG CCCAGGGACA ATCGGGACTC GGAGCAGCAG GAGTGGGATC CGGCGGTGGA 
TCTTCCGCGG CCACTGTGAC GACGGCCACC TCGAACAGCG GCACCGCCCA AGGACTGCAG 
TCGACGTCGG CCTCCGCGGA GGCCACGTCC TCGGCGGCCA CGTCTTCCTC CCAATCCTCT 
CTAACGCCTT CGCTGTCCTC GTCCCTGGCG AATGCCAATA ATGGAGGAGC CCGAACATTC 
CCTGCACGCA GTTTCCCACC GGACGGCACT ACCTTTGGCC AGATTACTCT CGGCCAGAAG 
CTGACCAAGG AGATCCAGCC GTACAGCTAC TGGAACATGC AGTTTTACCA GTCGGAACCT 
GCCTACGTTA AGTTCGACTA CACGATTCCG AGGGGCGCCT CCATCGGTGT ATACGGCCGA 
CGCAACGCCC TACCCACCCA CACGCAGTAC CACTTTAAGG AAGTGCTCAG TGGATTTAGT 
GCCAGCACAC GAACGGCCCG GGCCGCTCAC CTGTCGATAA CTCGGGAGGT GACACGCTAT 
ATGGAGCCGG GGCACTGGTT CGTCTCGCTT TACAACGACG ACGGGGACGT GCAGGAGCTG 
ACCTTCTACG CGGCCGTAGC CGAGGACATG ACCCAAAACT GTCCCAACGG CTGCTCCGGC 
AACGGTCAGT GCCTGCTGGG TCACTGCCAG TGCAACCCCG GCTTCGGGGG CGACGACTGC 
AGCGAGAGCG TGTGCCCCGT ACTGTGCTCC CAACATGGCG AGTACACCAA CGGAGAGTGC 
ATCTGTAACC CGGGCTGGAA GGGCAAGGAG TGCTCTCTGC GCCACGACGA GTGCGAGGTG 
GCCGACTGCA GTGGGCACGG CCACTGTGTC AGTGGAAAAT GCCAATGCAT GCGCGGCTAC 
AAAGGCAAAT TCTGCGAAGA AGTTGACTGC CCACATCCGA ACTGCTCAGG CCACGGATTC 
TGCGCCGATG GCACTTGCAT ATGCAAGAAG GGCTGGAAGG GACCTGACTG CGCTACCATG 
GACCAGGATG CGCTGCAGTG CCTCCCCGAC TGTTCCGGCC ACGGCACCTT CGACCTGGAC 
ACACAGACCT GTACCTGCGA GGCAAAGTGG AGTGGGGACG ATTGTTCCAA GGAGCTGTGC 
GACTTGGACT GCGGTCAGCA CGGACGTTGT GAAGGTGACG CTTGTGCATG CGACCCGGAG 
TGGGGTGGCG AGTATTGCAA CACCCGGTTG TGCGACGTCC GCTGTAACGA GCATGGCCAG 
TGCAAGAATG GTACTTGTCT GTGCGTTACT GGATGGAACG GAAAGCACTG CACCATCGAG 
GGCTGTCCCA ATAGCTGCGC CGGGCATGGC CAGTGCCGTG TAAGTGGCGA GGGTCAATGG 
GAGTGTCGCT GCTACGAGGG CTGGGACGGG CCGGACTGTG GGATTGCACT GGAGTTGAAC 
TGTGGCGACA GCAAGGACAA CGACAAGGAT GGCTTGGTTG ATTGCGAGGA TCCTGAGTGC 
TGTGCCAGCC ACGTATGCAA GACCTCCCAA CTATGTGTTT CAGCTCCGAA GCCCATTGAT 
GTGCTGCTCA GAAAACAGCC GCCAGCTATT ACGGCCTCTT TCTTTGAGCG GATGAAGTTC 
CTCATCGACG AGAGCAGCCT GCAGAACTAC GCCAAACTGG AAACCTTTAA CGAAAGTCGA 
TCAGCGGTGA TCAGAGGCCG CGTTGTTACA TCTCTGGGCA TGGGACTGGT GGGTGTGCGA 
GTGTCCACCA CCACGCTCCT AGAGGGCTTC ACCCTGACCC GAGATGATGG ATGGTTCGAC 
CTAATGGTTA ACGGTGGTGG AGCCGTAACG CTGCAGTTCG GACGCGCACC TTTCCGGCCG 
CAGTCGCGCA TCGTGCAAGT TCCATGGAAC GAGGTCGTGA TTATTGACTT GGTCGTCATG 
AGTATGTCCG AGGAAAAGGG ATTGGCCGTA ACTACGACAC ACACATGCTT TGCACACGAC 
TACGACCTGA TGAAGCCCGT GGTGCTGGCT TCGTGGAAGC ACGGGTTTCA GGGAGCCTGT 
CCCGATCGCA GCGCCATTCT TGCTGAATCT CAAGTGATTC AGGAGTCGTT GCAGATCCCG 
GGTACAGGCC TCAACCTGGT TTACCACTCA TCGCGTGCTG CCGGCTACCT TTCCACTATT 
AAGCTGCAGT TAACTCCGGA TGTGATTCCG ACATCGCTAC ATCTTATCCA TCTCCGCATA 
ACAATCGAGG GAATACTCTT TGAACGAATA TTCGAAGCAG ATCCGGGAAT TAAGTTCACG 
TACGCCTGGA ACCGACTCAA CATCTACCGG CAACGAGTCT ACGGTGTAAC CACGGCCGTG 
GTGAAGGTTG GCTACCAGTA CACCGACTGC ACTGACATTG TGTGGGACAT TCAAACAACT 
AAGCTAAGCG GTCACGACAT GTCGATCTCA GAAGTGGGTG GGTGGAACCT GGACATCCAT 
CACCGCTACA ACTTCCACGA GGGAATTCTG CAAAAGGGCG ACGGCTCAAA CATATATCTG 
CGCAATAAGC CGCGAATCAT CCTGACCACA ATGGGAGATG GCCACCAGCG GCCTCTGGAG 
TGTCCCGATT GCGACGGCCA GGCGACAAAG CAGCGACTTC TGGCACCCGT CGCACTGGCC 
GCCGCTCCTG ACGGCAGTCT TTTCGTCGGT GATTTTAATT ACATCCGCCG CATCATGACC 
GACGGCAGCA TCCGCACTGT GGTCAAGCTG AACGCGACTC GCGTCTCGTA CCGCTACCAC 
ATGGCCCTCA GCCCGCTCGA CGGAACTCTT TATGTTTCGG ATCCAGAATC ACACCAAATC 
ATTCGGGTAC GCGACACCAA CGACTACTCG CAACCGGAAC TAAACTGGGA AGCGGTTGTG 
GGCTCCGGGG AGCGCTGTCT TCCTGGTGAC GAAGCGCACT GCGGCGATGG GGCACTGGCA 
AAGGATGCCA AATTGGCTTA TCCGAAAGGC ATTGCGATTT CGAGCGACAA CATCTTGTAC 
TTTGCAGACG GAACTAATAT CCGTATGGTG GACCGGGATG GAATTGTAAG CACTCTGATC 
GGCAACCACA TGCACAAGTC CCACTGGAAG CCAATTCCCT GCGAGGGAAC CCTGAAGCTG 
GAGGAGATGC ATCTTCGCTG GCCCACTGAG CTAGCAGTCT CCCCGATGGA CAACACGCTG 
CACATAATCG ACGACCACAT GATCCTGCGG ATGACACCAG ACGGTCGTGT GCGCGTCATC 
TCCGGCCGTC CCCTACATTG CGCCACAGCC TCCACTGCCT ACGATACGGA TCTGGCTACC 
CACGCTACCC TGGTGATGCC GCAGTCTATC GCGTTCGGTC CACTCGGTGA GCTTTACGTG 
GCGGAAAGCG ACTCTCAACG AATAAACCGG GTGCGCGTGA TCGGCACGGA CGGAAGGATC 
GCTCCGTTTG CTGGTGCCGA ATCCAAGTGC AATTGCCTGG AGCGCGGTTG CGACTGCTTC 
GAAGCGGAAC ACTATCTGGC CACCAGTGCC AAGTTCAACA CGATCGCCGC CCTTGCTGTC 
ACACCCGATA GCCATGTACA CATCGCGGAC CAGGCCAACT ACCGTATCCG CTCCGTTATG 
TCGAGCATCC CAGAGGCAAG TCCGTCGCGC GAGTACGAGA TCTACGCACC TGACATGCAG 
GAGATATACA TTTTCAACCG ATTCGGACAG CACGTGTCCA CACGCAACAT CCTGACCGGA 
GAGACGACCT ATGTGTTTAC CTACAACGTA AACACCTCCA ACGGAAAACT GAGCACCGTA 
ACAGACGCGG CTGGCAACAA AGTGTTCCTG CTTCGAGATT ACACTTCTCA GGTAAATTCC 
ATCGAGAACA CGAAGGGTCA AAAGTGCCGC CTGCGCATGA CCAGGATGAA AATGCTGCAC 
GAGCTGAGCA CTCCAGACAA CTACAATGTG ACCTACGAAT ATCACGGCCC CACCGGTCTG 
CTACGAACCA AGCTGGACTC CACCGGACGA TCCTACGTTT ACAACTACGA TGAGTTTGGT 
CGCCTCACTT CTGCAGTGAC TCCCACCGGT CGTGTCATCG AGCTGAGCTT CGACCTGAGC 
GTGAAGGGAG CCCAGGTAAA GGTTTCGGAG AACGCCCAGA AGGAAATGTC ACTGCTGATC 
CAAGGTGCCA CCGTTATTGT GCGCAACGGA GCCGCCGAGT CGCGTACCAC CGTTGACATG 
GACGGCTCCA CCACAAGCAT CACTCCATGG GGCCACAACC TGCAGATGGA AGTGGCACCT 
TATACCATTC TGGCAGAACA AAGTCCACTG CTTGGCGAGA GCTACCCGGT CCCGGCTAAG 
CAACGCACTG AGATTGCTGG TGACTTGGCC AACAGGTTTG AGTGGCGCTA CTTTGTGCGC 
CGGCAGCAGC CGCTGCAAGC GGGAAAACAG AGCAAGGGAC CGCCACGTCC CGTAACCGAA 
GTTGGACGTA AGCTGCGTGT GAACGGAGAC AACGTGCTGA CTTTGGAGTA CGACCGCGAG 
ACCCAGTCGG TGGTCGTTAT GGTGGACGAC AAGCAGGAGC TGCTTAACGT GACCTACGAT 
CGCACTTCCC GCCCCATCAG CTTCCGACCG CAGTCCGGCG ACTACGCGGA CGTGGACTTG 
GAGTACGATC GGTTCGGACG TCTGGTCAGT TGGAAGTGGG GTGTCCTGCA AGAAGCCTAC 
TCTTTCGACC GCAATGGCCG TCTGAACGAG ATCAAGTACG GCGATGGCTC AACAATGGTC 
TACGCATTCA AGGACATGTT TGGCTCGTTA CCGCTGAAGG TGACTACGCC TAGACGTTCT 
GATTATCTCC TGCAGTACGA TGATGCAGGT GCACTCCAGA GCCTGACGAC ACCTCGTGGA 
CACATTCACG CATTCTCCCT GCAAACTTCT CTTGGCTTCT TTAAGTACCA ATACTATTCA 
CCAATCAACC GACACCCCTT CGAGATTCTA TATAACGACG AAGGACAGAT CTTGGCCAAA 
ATCCACCCCC ACCAATCTGG CAAGGTGGCA TTCGTACATG ATACTGCCGG ACGACTGGAG 
ACGATCCTCG CAGGATTGTC CAGCACCCAC TATACCTACC AGGATACAAC TAGCCTGGTA 
AAGTCCGTGG AGGTGCAGGA GCCGGGCTTC GAGTTGCGCC GAGAGTTTAA ATACCATGCA 
GGTATCCTAA AGGACGAGAA GTTGCGCTTC GGCTCCAAGA ACTCGCTGGC CTCGGCGCGC 
TACAAGTACG CCTACGACGG AAACGCCCGT CTCAGCGGCA TCGAGATGGC CATCGACGAT 
AAGGAATTAC CAACCACGCG GTACAAGTAC AGCCAAAACT TGGGACAACT GGAGGTCGTG 
CAGGACCTGA AGATCACGCG CAACGCTTTT AACCGAACAG TCATACAGGA CTCTGCCAAA 
CAATTCTTCG CCATTGTGGA CTACGACCAG CATGGACGGG TTAAGAGCGT GCTAATGAAC 
GTGAAGAACA TTGATGTGTT CCGCCTTGAG TTGGACTACG ATCTTCGCAA CCGCATCAAG 
TCGCAAAAGA CGACCTTCGG TAGATCGACA GCCTTTGATA AGATTAACTA CAATGCCGAC 
GGACATGTTG TTGAGGTCCT GGGCACCAAC AACTGGAAGT ATCTGTTCGA CGAGAACGGC 
AACACTGTTG GCGTGGTTGA CCAGGGTGAG AAGTTCAACC TGGGCTACGA CATCGGAGAC 
CGGGTGATCA AGGTAGGCGA TGTGGAGTTC AACAACTACG ATGCCCGCGG CTTCGTTGTG 
AAGCGCGGTG AGCAGAAGTA CCGCTACAAC AACCGCGGAC AACTGATTCA CTCGTTCGAA 
AGGGAACGTT TCCAGAGCTG GTACTACTAC GACGACCGCA GCCGCCTCGT GGCCTGGCAT 
GATAACAAAG GTAACACCAC GCAGTACTAC TACGCCAATC CTCGCACTCC TCATCTCGTG 
ACCCACGTTC ACTTCCCGAA GATCAGCCGC ACCATGAAGC TGTTCTACGA TGACCGCGAC 
ATGCTAATCG CTTTGGAGCA TGAGGACCAG CGCTACTACG TGGCCACCGA CCAGAACGGC 
TCGCCACTAG CCTTCTTTGA TCAGAATGGC AGCATTGTCA AGGAAATGAA GCGAACGCCC 
TTCGGCCGAA TCATTAAGGA CACTAAGCCG GAGTTCTTTG TGCCCATCGA CTTCCACGGC 
GGACTGATCG ACCCACACAC AAAGCTGGTG TACACCGAGC AGCGGCAGTA CGACCCGCAC 
GTTGGCCAAT GGATGACCCC GTTGTGGGAG ACGCTCGCCA CCGAGATGTC ACACCCGACG 
GATGTTTTTA TATACCGCTA CCACAACAAC GACCCCATCA ACCCGAACAA GCCCCAAAAC 
TATATGATCG ATCTGGATTC CTGGCTCCAG TTATTCGGCT ACGATTTGAA CAACATGCAA 
AGCAGCCGCT ACACCAAGCT GGCCCAGTAT ACGCCGCAGG CTTCCATCAA GTCAAACACG 
TTGGCCCCCG ACTTTGGCGT GATCTCCGGC CTGGAGTGCA TCGTGGAAAA AACAAGTGAG 
AAGTTCAGTG ATTTTGACTT TGTGCCCAAA CCGCTGCTGA AAATGGAGCC AAAGATGCGC 
AACTTGCTCC CGCGTGTCAG CTACCGGCGC GGTGTGTTCG GCGAAGGTGT ACTCCTCTCG 
AGAATCGGCG GACGGGCGTT AGTTAGCGTG GTCGACGGAT CAAACAGCGT GGTGCAGGAC 
GTAGTGAGCA GCGTGTTCAA CAACTCTTAC TTCCTTGACC TACACTTCAG CATTCACGAC 
CAAGACGTAT TTTACTTCGT AAAGGATAAT GTCCTGAAGC TGCGCGACGA CAACGAGGAG 
CTGCGTCGCC TTGGCGGCAT GTTCAACATA TCAACCCATG AAATCAGCGA CCACGGAGGC 
AGCGCCGCTA AGGAGCTGCG TCTTCACGGC CCAGACGCCG TTGTCATTAT CAAATATGGC 
GTTGATCCCG AGCAGGAGCG CCACCGCATC CTAAAGCATG CCCACAAGCG GGCAGTGGAG 
CGGGCTTGGG AGTTGGAGAA GCAGCTGGTG GCTGCCGGCT TCCAAGGCCG TGGCGACTGG 
ACCGAGGAGG AGAAAGAGGA GCTCGTCCAA CACGGTGACG TCGACGGCTG GAACGGAATC 
GATATCCACA GCATACACAA GTATCCGCAG CTGGCCGACG ACCCCGGTAA CGTGGCTTTC 
CAGCGGGACG CGAAGCGTAA GCGACGCAAG ACCGGCAGCA GCCATCGGAG TGCCTCCAAC 
CGCCGCCAGC TCAAGTTCGG CGAGCTGAGT GCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ten-m ( NM_001104191.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097661.2 Drosophila melanogaster tenascin major, transcript variant D (Ten-m), mRNA.

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CloneID	OFa19268
Clone ID Related Accession (Same CDS sequence)	NM_001104191.3
Accession Version	NM_001104191.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tenascin major, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001104191.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCCCT ACGAGTATGA GTCGACTCTA GACTGCCGCG ACGTGGGCGG CGGCCCCACT 
CCGGCTCACG CCCACCCACA CGCCCAAGGA CGTACGCTCC CCATGAGCGG CCACGGGCGG 
CCGACGACGG ACCTCGGTCC GGTCCACGGC AGCCAGACGC TCCAGCACCA AAACCAACAG 
AACCTGCAGG CCGCCCAAGC CGCCGCCCAG TCGTCCCACT ACGACTACGA GTACCAGCAC 
TTGGCCCACC GTCCGCCGGA CACGGCCAAC AACACAGCGC AGCGGACACA CGGAAGACAA 
GGCTTCCTCC TGGAAGGAGT CACGCCAACG GCACCACCGG ATGTTCCGCC GCGTAATCCG 
ACAATGAGTC GCATGCAAAA TGGTCGTTTA ACCGTTAACA ATCCCAATGA CGCTGACTTT 
GAGCCATCTT GTTTGGTGCG AACGCCATCG GGCAACGTTT ACATTCCTAG TGGAAATCTA 
AACATCAACA AGGGATCACC CATCGACTTC AAGAGCGGCT CGGCCTGCTC CACACCCACG 
AAGGATACGC TGAAGGGCTA CGAGCGGAGC ACGCAGGGCT GCATGGGTCC TGTGCTGCCG 
CAGCGCAGCG TCATGAACGG ACTGCCCGCA CACCACTACT CGGCGCCGAT GAACTTCCGC 
AAGGACCTGG TCGCGCGCTG CTCCTCGCCG TGGTTCGGTA TAGGATCCAT CTCGGTGCTC 
TTCGCCTTCG TGGTCATGCT TATCCTGCTA ACCACCACCG GCGTTATAAA ATGGAATCAG 
TCACCACCCT GCTCCGTTCT AGTCGGCAAC GAGGCCTCTG AGGTCACGGC TGCCAAGAGC 
ACGAACACGG ACCTCTCCAA GCTGCACAAT TCATCGGTGC GCGCGAAGAA CGGACAAGGA 
ATCGGACTGG CCCAGGGACA ATCGGGACTC GGAGCAGCAG GAGTGGGATC CGGCGGTGGA 
TCTTCCGCGG CCACTGTGAC GACGGCCACC TCGAACAGCG GCACCGCCCA AGGACTGCAG 
TCGACGTCGG CCTCCGCGGA GGCCACGTCC TCGGCGGCCA CGTCTTCCTC CCAATCCTCT 
CTAACGCCTT CGCTGTCCTC GTCCCTGGCG AATGCCAATA ATGGAGGACG AGCGATGTCA 
ACGCGGCTGT CAGTCCGTGG GGCAGGAGAA AGAGGACGTC GACATCGCCG AAGCCTAAAC 
GAGGAGCAGG GCGAAGATGA TGTGGCTACA GATGGAACTT TCAGTGACTT AATTACAAAC 
GAAAGCCTCA ACCAGCAGGC GGCTGAAAAG TATTTGGCAA CTACCCTAGC AAAAAGCCCG 
ACTGATGTTC ACGGCAGTGG CAATAAAACT TTACCCCGAA TGGATGGAGT ATACGGTACC 
CAAAGGAGCG AAGACACCCC GGACACCAGC TACGACTATG TCTATGAAGA CGAAGTTGAG 
CCGGAGACCA CCCCTTCGCT CATCCGTAGA ACCAAGACGG GGCAACAATT TGGCAAATCA 
TTAAACAGCA ATTTACGCAG CGCTGCTAAA ACACTGGTCA ACAAGAGGAG GAAATATGAC 
CACGGAACGG TGGAAGCGGA GCATATTAAG CATGAGGAGG AGGAGGAGGA GGATGAGCAG 
AAGCTGGAGC GCCACGAAGC GATCGGGATG GCAACGGAGC TGACGACGGA GTCGGAGACC 
AGCACATTGC CTGCTGTTAT TGATGACGAT AATCAAAGTG ACAACAGCAG CTCAGGCCCA 
ACGCCGGAGA CGACTGTAAG GAGCGACACC GATGACATTG TTGAAATCAA CACCCCCCCA 
TCACAGACAG CTCAAAGGAC TTTCGCCGCC GTCTCGCACC AGCCTGCCAT TGAACATGAT 
TTTCAAATCA AAGGGACCGA TGCTGGGGGC TTGCAGACGG AGAAACCTGC CACTGATGAT 
ATCAATAATG AGCGTGATTT AGCTGACAAC TATGAGGTAG ACAGTAAGGA GCCGACCTCG 
CCGGGCACAC CCCCACAAGG TAAGGTGAGT CAGCAAACTG GAAAAGCGTC ACTACAATCA 
CTTCAATCTG AATCGGATCT GATGATGAAT GATGCTTCGC ACTATGAGGA TATCGATATA 
GTAAAACTAG ATGGATTGAC GATCTCCCAC GAGGAGGAGA TTTACAAGAC GGCAGATAAG 
GAAAATATGG CACCAAAAAA TCAACCATCT CAGCATATAG ATAGAAGTCA AAACGAGGTA 
CTGAAAGGCC ATCAGCAGGG TGACGAAAAA CAACCCCAAC TAGAGCCCCT TAAACCGTAT 
GTGAGTGAGC GAGTTGATCT GCCGGGCAAA CGCATATTCC TGAATCTGAC TATAGCCACA 
GACGAGGGCA GTGACTCCGT TTACACCTTG CACGTCGAAG TGCCCACGGG CGGAGGACCC 
CACTTCATCA AGGAGGTCCT GACGCATGAG AAACCGACTG CCCAGGCAGA CAGTTGTGTT 
CCGGAACCAC CACCGCGCAT GCCTGACTGC CCGTGCAGTT GCCTCCCGCC TCCAGCGCCC 
ATCTATCTGG ATGACACCGT TGACATCGAC TCTGCCCCAC CAGCCAAAAC TGTTACGACG 
AGCACGATCT CGGCACCTAT TAACCCCTTC CATAGCGAGG AGGAGGATGA CGAAGACGGG 
GTTAGAGATG AGGAGCAAAC ACCTTCTTCA AGCACGGCTA CTAACCTCCC TAGCACTGAG 
ATCGATAACC ACATTGCCGC CTTTACTGAA CCCGCTGTTG GTGCTGGTGG TGTGCCGTTT 
GCATGCCCTG ATGTGATGCC AGTACTAATA CTGGAAGGAG CCCGAACATT CCCTGCACGC 
AGTTTCCCAC CGGACGGCAC TACCTTTGGC CAGATTACTC TCGGCCAGAA GCTGACCAAG 
GAGATCCAGC CGTACAGCTA CTGGAACATG CAGTTTTACC AGTCGGAACC TGCCTACGTT 
AAGTTCGACT ACACGATTCC GAGGGGCGCC TCCATCGGTG TATACGGCCG ACGCAACGCC 
CTACCCACCC ACACGCAGTA CCACTTTAAG GAAGTGCTCA GTGGATTTAG TGCCAGCACA 
CGAACGGCCC GGGCCGCTCA CCTGTCGATA ACTCGGGAGG TGACACGCTA TATGGAGCCG 
GGGCACTGGT TCGTCTCGCT TTACAACGAC GACGGGGACG TGCAGGAGCT GACCTTCTAC 
GCGGCCGTAG CCGAGGACAT GACCCAAAAC TGTCCCAACG GCTGCTCCGG CAACGGTCAG 
TGCCTGCTGG GTCACTGCCA GTGCAACCCC GGCTTCGGGG GCGACGACTG CAGCGAGAGC 
GTGTGCCCCG TACTGTGCTC CCAACATGGC GAGTACACCA ACGGAGAGTG CATCTGTAAC 
CCGGGCTGGA AGGGCAAGGA GTGCTCTCTG CGCCACGACG AGTGCGAGGT GGCCGACTGC 
AGTGGGCACG GCCACTGTGT CAGTGGAAAA TGCCAATGCA TGCGCGGCTA CAAAGGCAAA 
TTCTGCGAAG AAGTTGACTG CCCACATCCG AACTGCTCAG GCCACGGATT CTGCGCCGAT 
GGCACTTGCA TATGCAAGAA GGGCTGGAAG GGACCTGACT GCGCTACCAT GGACCAGGAT 
GCGCTGCAGT GCCTCCCCGA CTGTTCCGGC CACGGCACCT TCGACCTGGA CACACAGACC 
TGTACCTGCG AGGCAAAGTG GAGTGGGGAC GATTGTTCCA AGGAGCTGTG CGACTTGGAC 
TGCGGTCAGC ACGGACGTTG TGAAGGTGAC GCTTGTGCAT GCGACCCGGA GTGGGGTGGC 
GAGTATTGCA ACACCCGGTT GTGCGACGTC CGCTGTAACG AGCATGGCCA GTGCAAGAAT 
GGTACTTGTC TGTGCGTTAC TGGATGGAAC GGAAAGCACT GCACCATCGA GGGCTGTCCC 
AATAGCTGCG CCGGGCATGG CCAGTGCCGT GTAAGTGGCG AGGGTCAATG GGAGTGTCGC 
TGCTACGAGG GCTGGGACGG GCCGGACTGT GGGATTGCAC TGGAGTTGAA CTGTGGCGAC 
AGCAAGGACA ACGACAAGGA TGGCTTGGTT GATTGCGAGG ATCCTGAGTG CTGTGCCAGC 
CACGTATGCA AGACCTCCCA ACTATGTGTT TCAGCTCCGA AGCCCATTGA TGTGCTGCTC 
AGAAAACAGC CGCCAGCTAT TACGGCCTCT TTCTTTGAGC GGATGAAGTT CCTCATCGAC 
GAGAGCAGCC TGCAGAACTA CGCCAAACTG GAAACCTTTA ACGAAAGCAT TTTTTGGAAT 
TATTTCAATG CAAGTCGATC AGCGGTGATC AGAGGCCGCG TTGTTACATC TCTGGGCATG 
GGACTGGTGG GTGTGCGAGT GTCCACCACC ACGCTCCTAG AGGGCTTCAC CCTGACCCGA 
GATGATGGAT GGTTCGACCT AATGGTTAAC GGTGGTGGAG CCGTAACGCT GCAGTTCGGA 
CGCGCACCTT TCCGGCCGCA GTCGCGCATC GTGCAAGTTC CATGGAACGA GGTCGTGATT 
ATTGACTTGG TCGTCATGAG TATGTCCGAG GAAAAGGGAT TGGCCGTAAC TACGACACAC 
ACATGCTTTG CACACGACTA CGACCTGATG AAGCCCGTGG TGCTGGCTTC GTGGAAGCAC 
GGGTTTCAGG GAGCCTGTCC CGATCGCAGC GCCATTCTTG CTGAATCTCA AGTGATTCAG 
GAGTCGTTGC AGATCCCGGG TACAGGCCTC AACCTGGTTT ACCACTCATC GCGTGCTGCC 
GGCTACCTTT CCACTATTAA GCTGCAGTTA ACTCCGGATG TGATTCCGAC ATCGCTACAT 
CTTATCCATC TCCGCATAAC AATCGAGGGA ATACTCTTTG AACGAATATT CGAAGCAGAT 
CCGGGAATTA AGTTCACGTA CGCCTGGAAC CGACTCAACA TCTACCGGCA ACGAGTCTAC 
GGTGTAACCA CGGCCGTGGT GAAGGTTGGC TACCAGTACA CCGACTGCAC TGACATTGTG 
TGGGACATTC AAACAACTAA GCTAAGCGGT CACGACATGT CGATCTCAGA AGTGGGTGGG 
TGGAACCTGG ACATCCATCA CCGCTACAAC TTCCACGAGG GAATTCTGCA AAAGGGCGAC 
GGCTCAAACA TATATCTGCG CAATAAGCCG CGAATCATCC TGACCACAAT GGGAGATGGC 
CACCAGCGGC CTCTGGAGTG TCCCGATTGC GACGGCCAGG CGACAAAGCA GCGACTTCTG 
GCACCCGTCG CACTGGCCGC CGCTCCTGAC GGCAGTCTTT TCGTCGGTGA TTTTAATTAC 
ATCCGCCGCA TCATGACCGA CGGCAGCATC CGCACTGTGG TCAAGCTGAA CGCGACTCGC 
GTCTCGTACC GCTACCACAT GGCCCTCAGC CCGCTCGACG GAACTCTTTA TGTTTCGGAT 
CCAGAATCAC ACCAAATCAT TCGGGTACGC GACACCAACG ACTACTCGCA ACCGGAACTA 
AACTGGGAAG CGGTTGTGGG CTCCGGGGAG CGCTGTCTTC CTGGTGACGA AGCGCACTGC 
GGCGATGGGG CACTGGCAAA GGATGCCAAA TTGGCTTATC CGAAAGGCAT TGCGATTTCG 
AGCGACAACA TCTTGTACTT TGCAGACGGA ACTAATATCC GTATGGTGGA CCGGGATGGA 
ATTGTAAGCA CTCTGATCGG CAACCACATG CACAAGTCCC ACTGGAAGCC AATTCCCTGC 
GAGGGAACCC TGAAGCTGGA GGAGATGCAT CTTCGCTGGC CCACTGAGCT AGCAGTCTCC 
CCGATGGACA ACACGCTGCA CATAATCGAC GACCACATGA TCCTGCGGAT GACACCAGAC 
GGTCGTGTGC GCGTCATCTC CGGCCGTCCC CTACATTGCG CCACAGCCTC CACTGCCTAC 
GATACGGATC TGGCTACCCA CGCTACCCTG GTGATGCCGC AGTCTATCGC GTTCGGTCCA 
CTCGGTGAGC TTTACGTGGC GGAAAGCGAC TCTCAACGAA TAAACCGGGT GCGCGTGATC 
GGCACGGACG GAAGGATCGC TCCGTTTGCT GGTGCCGAAT CCAAGTGCAA TTGCCTGGAG 
CGCGGTTGCG ACTGCTTCGA AGCGGAACAC TATCTGGCCA CCAGTGCCAA GTTCAACACG 
ATCGCCGCCC TTGCTGTCAC ACCCGATAGC CATGTACACA TCGCGGACCA GGCCAACTAC 
CGTATCCGCT CCGTTATGTC GAGCATCCCA GAGGCAAGTC CGTCGCGCGA GTACGAGATC 
TACGCACCTG ACATGCAGGA GATATACATT TTCAACCGAT TCGGACAGCA CGTGTCCACA 
CGCAACATCC TGACCGGAGA GACGACCTAT GTGTTTACCT ACAACGTAAA CACCTCCAAC 
GGAAAACTGA GCACCGTAAC AGACGCGGCT GGCAACAAAG TGTTCCTGCT TCGAGATTAC 
ACTTCTCAGG TAAATTCCAT CGAGAACACG AAGGGTCAAA AGTGCCGCCT GCGCATGACC 
AGGATGAAAA TGCTGCACGA GCTGAGCACT CCAGACAACT ACAATGTGAC CTACGAATAT 
CACGGCCCCA CCGGTCTGCT ACGAACCAAG CTGGACTCCA CCGGACGATC CTACGTTTAC 
AACTACGATG AGTTTGGTCG CCTCACTTCT GCAGTGACTC CCACCGGTCG TGTCATCGAG 
CTGAGCTTCG ACCTGAGCGT GAAGGGAGCC CAGGTAAAGG TTTCGGAGAA CGCCCAGAAG 
GAAATGTCAC TGCTGATCCA AGGTGCCACC GTTATTGTGC GCAACGGAGC CGCCGAGTCG 
CGTACCACCG TTGACATGGA CGGCTCCACC ACAAGCATCA CTCCATGGGG CCACAACCTG 
CAGATGGAAG TGGCACCTTA TACCATTCTG GCAGAACAAA GTCCACTGCT TGGCGAGAGC 
TACCCGGTCC CGGCTAAGCA ACGCACTGAG ATTGCTGGTG ACTTGGCCAA CAGGTTTGAG 
TGGCGCTACT TTGTGCGCCG GCAGCAGCCG CTGCAAGCGG GAAAACAGAG CAAGGGACCG 
CCACGTCCCG TAACCGAAGT TGGACGTAAG CTGCGTGTGA ACGGAGACAA CGTGCTGACT 
TTGGAGTACG ACCGCGAGAC CCAGTCGGTG GTCGTTATGG TGGACGACAA GCAGGAGCTG 
CTTAACGTGA CCTACGATCG CACTTCCCGC CCCATCAGCT TCCGACCGCA GTCCGGCGAC 
TACGCGGACG TGGACTTGGA GTACGATCGG TTCGGACGTC TGGTCAGTTG GAAGTGGGGT 
GTCCTGCAAG AAGCCTACTC TTTCGACCGC AATGGCCGTC TGAACGAGAT CAAGTACGGC 
GATGGCTCAA CAATGGTCTA CGCATTCAAG GACATGTTTG GCTCGTTACC GCTGAAGGTG 
ACTACGCCTA GACGTTCTGA TTATCTCCTG CAGTACGATG ATGCAGGTGC ACTCCAGAGC 
CTGACGACAC CTCGTGGACA CATTCACGCA TTCTCCCTGC AAACTTCTCT TGGCTTCTTT 
AAGTACCAAT ACTATTCACC AATCAACCGA CACCCCTTCG AGATTCTATA TAACGACGAA 
GGACAGATCT TGGCCAAAAT CCACCCCCAC CAATCTGGCA AGGTGGCATT CGTACATGAT 
ACTGCCGGAC GACTGGAGAC GATCCTCGCA GGATTGTCCA GCACCCACTA TACCTACCAG 
GATACAACTA GCCTGGTAAA GTCCGTGGAG GTGCAGGAGC CGGGCTTCGA GTTGCGCCGA 
GAGTTTAAAT ACCATGCAGG TATCCTAAAG GACGAGAAGT TGCGCTTCGG CTCCAAGAAC 
TCGCTGGCCT CGGCGCGCTA CAAGTACGCC TACGACGGAA ACGCCCGTCT CAGCGGCATC 
GAGATGGCCA TCGACGATAA GGAATTACCA ACCACGCGGT ACAAGTACAG CCAAAACTTG 
GGACAACTGG AGGTCGTGCA GGACCTGAAG ATCACGCGCA ACGCTTTTAA CCGAACAGTC 
ATACAGGACT CTGCCAAACA ATTCTTCGCC ATTGTGGACT ACGACCAGCA TGGACGGGTT 
AAGAGCGTGC TAATGAACGT GAAGAACATT GATGTGTTCC GCCTTGAGTT GGACTACGAT 
CTTCGCAACC GCATCAAGTC GCAAAAGACG ACCTTCGGTA GATCGACAGC CTTTGATAAG 
ATTAACTACA ATGCCGACGG ACATGTTGTT GAGGTCCTGG GCACCAACAA CTGGAAGTAT 
CTGTTCGACG AGAACGGCAA CACTGTTGGC GTGGTTGACC AGGGTGAGAA GTTCAACCTG 
GGCTACGACA TCGGAGACCG GGTGATCAAG GTAGGCGATG TGGAGTTCAA CAACTACGAT 
GCCCGCGGCT TCGTTGTGAA GCGCGGTGAG CAGAAGTACC GCTACAACAA CCGCGGACAA 
CTGATTCACT CGTTCGAAAG GGAACGTTTC CAGAGCTGGT ACTACTACGA CGACCGCAGC 
CGCCTCGTGG CCTGGCATGA TAACAAAGGT AACACCACGC AGTACTACTA CGCCAATCCT 
CGCACTCCTC ATCTCGTGAC CCACGTTCAC TTCCCGAAGA TCAGCCGCAC CATGAAGCTG 
TTCTACGATG ACCGCGACAT GCTAATCGCT TTGGAGCATG AGGACCAGCG CTACTACGTG 
GCCACCGACC AGAACGGCTC GCCACTAGCC TTCTTTGATC AGAATGGCAG CATTGTCAAG 
GAAATGAAGC GAACGCCCTT CGGCCGAATC ATTAAGGACA CTAAGCCGGA GTTCTTTGTG 
CCCATCGACT TCCACGGCGG ACTGATCGAC CCACACACAA AGCTGGTGTA CACCGAGCAG 
CGGCAGTACG ACCCGCACGT TGGCCAATGG ATGACCCCGT TGTGGGAGAC GCTCGCCACC 
GAGATGTCAC ACCCGACGGA TGTTTTTATA TACCGCTACC ACAACAACGA CCCCATCAAC 
CCGAACAAGC CCCAAAACTA TATGATCGAT CTGGATTCCT GGCTCCAGTT ATTCGGCTAC 
GATTTGAACA ACATGCAAAG CAGCCGCTAC ACCAAGCTGG CCCAGTATAC GCCGCAGGCT 
TCCATCAAGT CAAACACGTT GGCCCCCGAC TTTGGCGTGA TCTCCGGCCT GGAGTGCATC 
GTGGAAAAAA CAAGTGAGAA GTTCAGTGAT TTTGACTTTG TGCCCAAACC GCTGCTGAAA 
ATGGAGCCAA AGATGCGCAA CTTGCTCCCG CGTGTCAGCT ACCGGCGCGG TGTGTTCGGC 
GAAGGTGTAC TCCTCTCGAG AATCGGCGGA CGGGCGTTAG TTAGCGTGGT CGACGGATCA 
AACAGCGTGG TGCAGGACGT AGTGAGCAGC GTGTTCAACA ACTCTTACTT CCTTGACCTA 
CACTTCAGCA TTCACGACCA AGACGTATTT TACTTCGTAA AGGATAATGT CCTGAAGCTG 
CGCGACGACA ACGAGGAGCT GCGTCGCCTT GGCGGCATGT TCAACATATC AACCCATGAA 
ATCAGCGACC ACGGAGGCAG CGCCGCTAAG GAGCTGCGTC TTCACGGCCC AGACGCCGTT 
GTCATTATCA AATATGGCGT TGATCCCGAG CAGGAGCGCC ACCGCATCCT AAAGCATGCC 
CACAAGCGGG CAGTGGAGCG GGCTTGGGAG TTGGAGAAGC AGCTGGTGGC TGCCGGCTTC 
CAAGGCCGTG GCGACTGGAC CGAGGAGGAG AAAGAGGAGC TCGTCCAACA CGGTGACGTC 
GACGGCTGGA ACGGAATCGA TATCCACAGC ATACACAAGT ATCCGCAGCT GGCCGACGAC 
CCCGGTAACG TGGCTTTCCA GCGGGACGCG AAGCGTAAGC GACGCAAGAC CGGCAGCAGC 
CATCGGAGTG CCTCCAACCG CCGCCAGCTC AAGTTCGGCG AGCTGAGTGC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097661.2
CDS	672..10565
Translation	MNPYEYESTLDCRDVGGGPTPAHAHPHAQGRTLPMSGHGRPTTDLGPVHGSQTLQHQNQQNLQAAQAAAQSSHYDYEYQHLAHRPPDTANNTAQRTHGRQGFLLEGVTPTAPPDVPPRNPTMSRMQNGRLTVNNPNDADFEPSCLVRTPSGNVYIPSGNLNINKGSPIDFKSGSACSTPTKDTLKGYERSTQGCMGPVLPQRSVMNGLPAHHYSAPMNFRKDLVARCSSPWFGIGSISVLFAFVVMLILLTTTGVIKWNQSPPCSVLVGNEASEVTAAKSTNTDLSKLHNSSVRAKNGQGIGLAQGQSGLGAAGVGSGGGSSAATVTTATSNSGTAQGLQSTSASAEATSSAATSSSQSSLTPSLSSSLANANNGGRAMSTRLSVRGAGERGRRHRRSLNEEQGEDDVATDGTFSDLITNESLNQQAAEKYLATTLAKSPTDVHGSGNKTLPRMDGVYGTQRSEDTPDTSYDYVYEDEVEPETTPSLIRRTKTGQQFGKSLNSNLRSAAKTLVNKRRKYDHGTVEAEHIKHEEEEEEDEQKLERHEAIGMATELTTESETSTLPAVIDDDNQSDNSSSGPTPETTVRSDTDDIVEINTPPSQTAQRTFAAVSHQPAIEHDFQIKGTDAGGLQTEKPATDDINNERDLADNYEVDSKEPTSPGTPPQGKVSQQTGKASLQSLQSESDLMMNDASHYEDIDIVKLDGLTISHEEEIYKTADKENMAPKNQPSQHIDRSQNEVLKGHQQGDEKQPQLEPLKPYVSERVDLPGKRIFLNLTIATDEGSDSVYTLHVEVPTGGGPHFIKEVLTHEKPTAQADSCVPEPPPRMPDCPCSCLPPPAPIYLDDTVDIDSAPPAKTVTTSTISAPINPFHSEEEDDEDGVRDEEQTPSSSTATNLPSTEIDNHIAAFTEPAVGAGGVPFACPDVMPVLILEGARTFPARSFPPDGTTFGQITLGQKLTKEIQPYSYWNMQFYQSEPAYVKFDYTIPRGASIGVYGRRNALPTHTQYHFKEVLSGFSASTRTARAAHLSITREVTRYMEPGHWFVSLYNDDGDVQELTFYAAVAEDMTQNCPNGCSGNGQCLLGHCQCNPGFGGDDCSESVCPVLCSQHGEYTNGECICNPGWKGKECSLRHDECEVADCSGHGHCVSGKCQCMRGYKGKFCEEVDCPHPNCSGHGFCADGTCICKKGWKGPDCATMDQDALQCLPDCSGHGTFDLDTQTCTCEAKWSGDDCSKELCDLDCGQHGRCEGDACACDPEWGGEYCNTRLCDVRCNEHGQCKNGTCLCVTGWNGKHCTIEGCPNSCAGHGQCRVSGEGQWECRCYEGWDGPDCGIALELNCGDSKDNDKDGLVDCEDPECCASHVCKTSQLCVSAPKPIDVLLRKQPPAITASFFERMKFLIDESSLQNYAKLETFNESIFWNYFNASRSAVIRGRVVTSLGMGLVGVRVSTTTLLEGFTLTRDDGWFDLMVNGGGAVTLQFGRAPFRPQSRIVQVPWNEVVIIDLVVMSMSEEKGLAVTTTHTCFAHDYDLMKPVVLASWKHGFQGACPDRSAILAESQVIQESLQIPGTGLNLVYHSSRAAGYLSTIKLQLTPDVIPTSLHLIHLRITIEGILFERIFEADPGIKFTYAWNRLNIYRQRVYGVTTAVVKVGYQYTDCTDIVWDIQTTKLSGHDMSISEVGGWNLDIHHRYNFHEGILQKGDGSNIYLRNKPRIILTTMGDGHQRPLECPDCDGQATKQRLLAPVALAAAPDGSLFVGDFNYIRRIMTDGSIRTVVKLNATRVSYRYHMALSPLDGTLYVSDPESHQIIRVRDTNDYSQPELNWEAVVGSGERCLPGDEAHCGDGALAKDAKLAYPKGIAISSDNILYFADGTNIRMVDRDGIVSTLIGNHMHKSHWKPIPCEGTLKLEEMHLRWPTELAVSPMDNTLHIIDDHMILRMTPDGRVRVISGRPLHCATASTAYDTDLATHATLVMPQSIAFGPLGELYVAESDSQRINRVRVIGTDGRIAPFAGAESKCNCLERGCDCFEAEHYLATSAKFNTIAALAVTPDSHVHIADQANYRIRSVMSSIPEASPSREYEIYAPDMQEIYIFNRFGQHVSTRNILTGETTYVFTYNVNTSNGKLSTVTDAAGNKVFLLRDYTSQVNSIENTKGQKCRLRMTRMKMLHELSTPDNYNVTYEYHGPTGLLRTKLDSTGRSYVYNYDEFGRLTSAVTPTGRVIELSFDLSVKGAQVKVSENAQKEMSLLIQGATVIVRNGAAESRTTVDMDGSTTSITPWGHNLQMEVAPYTILAEQSPLLGESYPVPAKQRTEIAGDLANRFEWRYFVRRQQPLQAGKQSKGPPRPVTEVGRKLRVNGDNVLTLEYDRETQSVVVMVDDKQELLNVTYDRTSRPISFRPQSGDYADVDLEYDRFGRLVSWKWGVLQEAYSFDRNGRLNEIKYGDGSTMVYAFKDMFGSLPLKVTTPRRSDYLLQYDDAGALQSLTTPRGHIHAFSLQTSLGFFKYQYYSPINRHPFEILYNDEGQILAKIHPHQSGKVAFVHDTAGRLETILAGLSSTHYTYQDTTSLVKSVEVQEPGFELRREFKYHAGILKDEKLRFGSKNSLASARYKYAYDGNARLSGIEMAIDDKELPTTRYKYSQNLGQLEVVQDLKITRNAFNRTVIQDSAKQFFAIVDYDQHGRVKSVLMNVKNIDVFRLELDYDLRNRIKSQKTTFGRSTAFDKINYNADGHVVEVLGTNNWKYLFDENGNTVGVVDQGEKFNLGYDIGDRVIKVGDVEFNNYDARGFVVKRGEQKYRYNNRGQLIHSFERERFQSWYYYDDRSRLVAWHDNKGNTTQYYYANPRTPHLVTHVHFPKISRTMKLFYDDRDMLIALEHEDQRYYVATDQNGSPLAFFDQNGSIVKEMKRTPFGRIIKDTKPEFFVPIDFHGGLIDPHTKLVYTEQRQYDPHVGQWMTPLWETLATEMSHPTDVFIYRYHNNDPINPNKPQNYMIDLDSWLQLFGYDLNNMQSSRYTKLAQYTPQASIKSNTLAPDFGVISGLECIVEKTSEKFSDFDFVPKPLLKMEPKMRNLLPRVSYRRGVFGEGVLLSRIGGRALVSVVDGSNSVVQDVVSSVFNNSYFLDLHFSIHDQDVFYFVKDNVLKLRDDNEELRRLGGMFNISTHEISDHGGSAAKELRLHGPDAVVIIKYGVDPEQERHRILKHAHKRAVERAWELEKQLVAAGFQGRGDWTEEEKEELVQHGDVDGWNGIDIHSIHKYPQLADDPGNVAFQRDAKRKRRKTGSSHRSASNRRQLKFGELSA

Target ORF information:

RefSeq Version	NM_001104191.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tenascin major, transcript variant D (Ten-m), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001104191.3

ORF Insert Sequence:

ATGAATCCCT ACGAGTATGA GTCGACTCTA GACTGCCGCG ACGTGGGCGG CGGCCCCACT 
CCGGCTCACG CCCACCCACA CGCCCAAGGA CGTACGCTCC CCATGAGCGG CCACGGGCGG 
CCGACGACGG ACCTCGGTCC GGTCCACGGC AGCCAGACGC TCCAGCACCA AAACCAACAG 
AACCTGCAGG CCGCCCAAGC CGCCGCCCAG TCGTCCCACT ACGACTACGA GTACCAGCAC 
TTGGCCCACC GTCCGCCGGA CACGGCCAAC AACACAGCGC AGCGGACACA CGGAAGACAA 
GGCTTCCTCC TGGAAGGAGT CACGCCAACG GCACCACCGG ATGTTCCGCC GCGTAATCCG 
ACAATGAGTC GCATGCAAAA TGGTCGTTTA ACCGTTAACA ATCCCAATGA CGCTGACTTT 
GAGCCATCTT GTTTGGTGCG AACGCCATCG GGCAACGTTT ACATTCCTAG TGGAAATCTA 
AACATCAACA AGGGATCACC CATCGACTTC AAGAGCGGCT CGGCCTGCTC CACACCCACG 
AAGGATACGC TGAAGGGCTA CGAGCGGAGC ACGCAGGGCT GCATGGGTCC TGTGCTGCCG 
CAGCGCAGCG TCATGAACGG ACTGCCCGCA CACCACTACT CGGCGCCGAT GAACTTCCGC 
AAGGACCTGG TCGCGCGCTG CTCCTCGCCG TGGTTCGGTA TAGGATCCAT CTCGGTGCTC 
TTCGCCTTCG TGGTCATGCT TATCCTGCTA ACCACCACCG GCGTTATAAA ATGGAATCAG 
TCACCACCCT GCTCCGTTCT AGTCGGCAAC GAGGCCTCTG AGGTCACGGC TGCCAAGAGC 
ACGAACACGG ACCTCTCCAA GCTGCACAAT TCATCGGTGC GCGCGAAGAA CGGACAAGGA 
ATCGGACTGG CCCAGGGACA ATCGGGACTC GGAGCAGCAG GAGTGGGATC CGGCGGTGGA 
TCTTCCGCGG CCACTGTGAC GACGGCCACC TCGAACAGCG GCACCGCCCA AGGACTGCAG 
TCGACGTCGG CCTCCGCGGA GGCCACGTCC TCGGCGGCCA CGTCTTCCTC CCAATCCTCT 
CTAACGCCTT CGCTGTCCTC GTCCCTGGCG AATGCCAATA ATGGAGGACG AGCGATGTCA 
ACGCGGCTGT CAGTCCGTGG GGCAGGAGAA AGAGGACGTC GACATCGCCG AAGCCTAAAC 
GAGGAGCAGG GCGAAGATGA TGTGGCTACA GATGGAACTT TCAGTGACTT AATTACAAAC 
GAAAGCCTCA ACCAGCAGGC GGCTGAAAAG TATTTGGCAA CTACCCTAGC AAAAAGCCCG 
ACTGATGTTC ACGGCAGTGG CAATAAAACT TTACCCCGAA TGGATGGAGT ATACGGTACC 
CAAAGGAGCG AAGACACCCC GGACACCAGC TACGACTATG TCTATGAAGA CGAAGTTGAG 
CCGGAGACCA CCCCTTCGCT CATCCGTAGA ACCAAGACGG GGCAACAATT TGGCAAATCA 
TTAAACAGCA ATTTACGCAG CGCTGCTAAA ACACTGGTCA ACAAGAGGAG GAAATATGAC 
CACGGAACGG TGGAAGCGGA GCATATTAAG CATGAGGAGG AGGAGGAGGA GGATGAGCAG 
AAGCTGGAGC GCCACGAAGC GATCGGGATG GCAACGGAGC TGACGACGGA GTCGGAGACC 
AGCACATTGC CTGCTGTTAT TGATGACGAT AATCAAAGTG ACAACAGCAG CTCAGGCCCA 
ACGCCGGAGA CGACTGTAAG GAGCGACACC GATGACATTG TTGAAATCAA CACCCCCCCA 
TCACAGACAG CTCAAAGGAC TTTCGCCGCC GTCTCGCACC AGCCTGCCAT TGAACATGAT 
TTTCAAATCA AAGGGACCGA TGCTGGGGGC TTGCAGACGG AGAAACCTGC CACTGATGAT 
ATCAATAATG AGCGTGATTT AGCTGACAAC TATGAGGTAG ACAGTAAGGA GCCGACCTCG 
CCGGGCACAC CCCCACAAGG TAAGGTGAGT CAGCAAACTG GAAAAGCGTC ACTACAATCA 
CTTCAATCTG AATCGGATCT GATGATGAAT GATGCTTCGC ACTATGAGGA TATCGATATA 
GTAAAACTAG ATGGATTGAC GATCTCCCAC GAGGAGGAGA TTTACAAGAC GGCAGATAAG 
GAAAATATGG CACCAAAAAA TCAACCATCT CAGCATATAG ATAGAAGTCA AAACGAGGTA 
CTGAAAGGCC ATCAGCAGGG TGACGAAAAA CAACCCCAAC TAGAGCCCCT TAAACCGTAT 
GTGAGTGAGC GAGTTGATCT GCCGGGCAAA CGCATATTCC TGAATCTGAC TATAGCCACA 
GACGAGGGCA GTGACTCCGT TTACACCTTG CACGTCGAAG TGCCCACGGG CGGAGGACCC 
CACTTCATCA AGGAGGTCCT GACGCATGAG AAACCGACTG CCCAGGCAGA CAGTTGTGTT 
CCGGAACCAC CACCGCGCAT GCCTGACTGC CCGTGCAGTT GCCTCCCGCC TCCAGCGCCC 
ATCTATCTGG ATGACACCGT TGACATCGAC TCTGCCCCAC CAGCCAAAAC TGTTACGACG 
AGCACGATCT CGGCACCTAT TAACCCCTTC CATAGCGAGG AGGAGGATGA CGAAGACGGG 
GTTAGAGATG AGGAGCAAAC ACCTTCTTCA AGCACGGCTA CTAACCTCCC TAGCACTGAG 
ATCGATAACC ACATTGCCGC CTTTACTGAA CCCGCTGTTG GTGCTGGTGG TGTGCCGTTT 
GCATGCCCTG ATGTGATGCC AGTACTAATA CTGGAAGGAG CCCGAACATT CCCTGCACGC 
AGTTTCCCAC CGGACGGCAC TACCTTTGGC CAGATTACTC TCGGCCAGAA GCTGACCAAG 
GAGATCCAGC CGTACAGCTA CTGGAACATG CAGTTTTACC AGTCGGAACC TGCCTACGTT 
AAGTTCGACT ACACGATTCC GAGGGGCGCC TCCATCGGTG TATACGGCCG ACGCAACGCC 
CTACCCACCC ACACGCAGTA CCACTTTAAG GAAGTGCTCA GTGGATTTAG TGCCAGCACA 
CGAACGGCCC GGGCCGCTCA CCTGTCGATA ACTCGGGAGG TGACACGCTA TATGGAGCCG 
GGGCACTGGT TCGTCTCGCT TTACAACGAC GACGGGGACG TGCAGGAGCT GACCTTCTAC 
GCGGCCGTAG CCGAGGACAT GACCCAAAAC TGTCCCAACG GCTGCTCCGG CAACGGTCAG 
TGCCTGCTGG GTCACTGCCA GTGCAACCCC GGCTTCGGGG GCGACGACTG CAGCGAGAGC 
GTGTGCCCCG TACTGTGCTC CCAACATGGC GAGTACACCA ACGGAGAGTG CATCTGTAAC 
CCGGGCTGGA AGGGCAAGGA GTGCTCTCTG CGCCACGACG AGTGCGAGGT GGCCGACTGC 
AGTGGGCACG GCCACTGTGT CAGTGGAAAA TGCCAATGCA TGCGCGGCTA CAAAGGCAAA 
TTCTGCGAAG AAGTTGACTG CCCACATCCG AACTGCTCAG GCCACGGATT CTGCGCCGAT 
GGCACTTGCA TATGCAAGAA GGGCTGGAAG GGACCTGACT GCGCTACCAT GGACCAGGAT 
GCGCTGCAGT GCCTCCCCGA CTGTTCCGGC CACGGCACCT TCGACCTGGA CACACAGACC 
TGTACCTGCG AGGCAAAGTG GAGTGGGGAC GATTGTTCCA AGGAGCTGTG CGACTTGGAC 
TGCGGTCAGC ACGGACGTTG TGAAGGTGAC GCTTGTGCAT GCGACCCGGA GTGGGGTGGC 
GAGTATTGCA ACACCCGGTT GTGCGACGTC CGCTGTAACG AGCATGGCCA GTGCAAGAAT 
GGTACTTGTC TGTGCGTTAC TGGATGGAAC GGAAAGCACT GCACCATCGA GGGCTGTCCC 
AATAGCTGCG CCGGGCATGG CCAGTGCCGT GTAAGTGGCG AGGGTCAATG GGAGTGTCGC 
TGCTACGAGG GCTGGGACGG GCCGGACTGT GGGATTGCAC TGGAGTTGAA CTGTGGCGAC 
AGCAAGGACA ACGACAAGGA TGGCTTGGTT GATTGCGAGG ATCCTGAGTG CTGTGCCAGC 
CACGTATGCA AGACCTCCCA ACTATGTGTT TCAGCTCCGA AGCCCATTGA TGTGCTGCTC 
AGAAAACAGC CGCCAGCTAT TACGGCCTCT TTCTTTGAGC GGATGAAGTT CCTCATCGAC 
GAGAGCAGCC TGCAGAACTA CGCCAAACTG GAAACCTTTA ACGAAAGCAT TTTTTGGAAT 
TATTTCAATG CAAGTCGATC AGCGGTGATC AGAGGCCGCG TTGTTACATC TCTGGGCATG 
GGACTGGTGG GTGTGCGAGT GTCCACCACC ACGCTCCTAG AGGGCTTCAC CCTGACCCGA 
GATGATGGAT GGTTCGACCT AATGGTTAAC GGTGGTGGAG CCGTAACGCT GCAGTTCGGA 
CGCGCACCTT TCCGGCCGCA GTCGCGCATC GTGCAAGTTC CATGGAACGA GGTCGTGATT 
ATTGACTTGG TCGTCATGAG TATGTCCGAG GAAAAGGGAT TGGCCGTAAC TACGACACAC 
ACATGCTTTG CACACGACTA CGACCTGATG AAGCCCGTGG TGCTGGCTTC GTGGAAGCAC 
GGGTTTCAGG GAGCCTGTCC CGATCGCAGC GCCATTCTTG CTGAATCTCA AGTGATTCAG 
GAGTCGTTGC AGATCCCGGG TACAGGCCTC AACCTGGTTT ACCACTCATC GCGTGCTGCC 
GGCTACCTTT CCACTATTAA GCTGCAGTTA ACTCCGGATG TGATTCCGAC ATCGCTACAT 
CTTATCCATC TCCGCATAAC AATCGAGGGA ATACTCTTTG AACGAATATT CGAAGCAGAT 
CCGGGAATTA AGTTCACGTA CGCCTGGAAC CGACTCAACA TCTACCGGCA ACGAGTCTAC 
GGTGTAACCA CGGCCGTGGT GAAGGTTGGC TACCAGTACA CCGACTGCAC TGACATTGTG 
TGGGACATTC AAACAACTAA GCTAAGCGGT CACGACATGT CGATCTCAGA AGTGGGTGGG 
TGGAACCTGG ACATCCATCA CCGCTACAAC TTCCACGAGG GAATTCTGCA AAAGGGCGAC 
GGCTCAAACA TATATCTGCG CAATAAGCCG CGAATCATCC TGACCACAAT GGGAGATGGC 
CACCAGCGGC CTCTGGAGTG TCCCGATTGC GACGGCCAGG CGACAAAGCA GCGACTTCTG 
GCACCCGTCG CACTGGCCGC CGCTCCTGAC GGCAGTCTTT TCGTCGGTGA TTTTAATTAC 
ATCCGCCGCA TCATGACCGA CGGCAGCATC CGCACTGTGG TCAAGCTGAA CGCGACTCGC 
GTCTCGTACC GCTACCACAT GGCCCTCAGC CCGCTCGACG GAACTCTTTA TGTTTCGGAT 
CCAGAATCAC ACCAAATCAT TCGGGTACGC GACACCAACG ACTACTCGCA ACCGGAACTA 
AACTGGGAAG CGGTTGTGGG CTCCGGGGAG CGCTGTCTTC CTGGTGACGA AGCGCACTGC 
GGCGATGGGG CACTGGCAAA GGATGCCAAA TTGGCTTATC CGAAAGGCAT TGCGATTTCG 
AGCGACAACA TCTTGTACTT TGCAGACGGA ACTAATATCC GTATGGTGGA CCGGGATGGA 
ATTGTAAGCA CTCTGATCGG CAACCACATG CACAAGTCCC ACTGGAAGCC AATTCCCTGC 
GAGGGAACCC TGAAGCTGGA GGAGATGCAT CTTCGCTGGC CCACTGAGCT AGCAGTCTCC 
CCGATGGACA ACACGCTGCA CATAATCGAC GACCACATGA TCCTGCGGAT GACACCAGAC 
GGTCGTGTGC GCGTCATCTC CGGCCGTCCC CTACATTGCG CCACAGCCTC CACTGCCTAC 
GATACGGATC TGGCTACCCA CGCTACCCTG GTGATGCCGC AGTCTATCGC GTTCGGTCCA 
CTCGGTGAGC TTTACGTGGC GGAAAGCGAC TCTCAACGAA TAAACCGGGT GCGCGTGATC 
GGCACGGACG GAAGGATCGC TCCGTTTGCT GGTGCCGAAT CCAAGTGCAA TTGCCTGGAG 
CGCGGTTGCG ACTGCTTCGA AGCGGAACAC TATCTGGCCA CCAGTGCCAA GTTCAACACG 
ATCGCCGCCC TTGCTGTCAC ACCCGATAGC CATGTACACA TCGCGGACCA GGCCAACTAC 
CGTATCCGCT CCGTTATGTC GAGCATCCCA GAGGCAAGTC CGTCGCGCGA GTACGAGATC 
TACGCACCTG ACATGCAGGA GATATACATT TTCAACCGAT TCGGACAGCA CGTGTCCACA 
CGCAACATCC TGACCGGAGA GACGACCTAT GTGTTTACCT ACAACGTAAA CACCTCCAAC 
GGAAAACTGA GCACCGTAAC AGACGCGGCT GGCAACAAAG TGTTCCTGCT TCGAGATTAC 
ACTTCTCAGG TAAATTCCAT CGAGAACACG AAGGGTCAAA AGTGCCGCCT GCGCATGACC 
AGGATGAAAA TGCTGCACGA GCTGAGCACT CCAGACAACT ACAATGTGAC CTACGAATAT 
CACGGCCCCA CCGGTCTGCT ACGAACCAAG CTGGACTCCA CCGGACGATC CTACGTTTAC 
AACTACGATG AGTTTGGTCG CCTCACTTCT GCAGTGACTC CCACCGGTCG TGTCATCGAG 
CTGAGCTTCG ACCTGAGCGT GAAGGGAGCC CAGGTAAAGG TTTCGGAGAA CGCCCAGAAG 
GAAATGTCAC TGCTGATCCA AGGTGCCACC GTTATTGTGC GCAACGGAGC CGCCGAGTCG 
CGTACCACCG TTGACATGGA CGGCTCCACC ACAAGCATCA CTCCATGGGG CCACAACCTG 
CAGATGGAAG TGGCACCTTA TACCATTCTG GCAGAACAAA GTCCACTGCT TGGCGAGAGC 
TACCCGGTCC CGGCTAAGCA ACGCACTGAG ATTGCTGGTG ACTTGGCCAA CAGGTTTGAG 
TGGCGCTACT TTGTGCGCCG GCAGCAGCCG CTGCAAGCGG GAAAACAGAG CAAGGGACCG 
CCACGTCCCG TAACCGAAGT TGGACGTAAG CTGCGTGTGA ACGGAGACAA CGTGCTGACT 
TTGGAGTACG ACCGCGAGAC CCAGTCGGTG GTCGTTATGG TGGACGACAA GCAGGAGCTG 
CTTAACGTGA CCTACGATCG CACTTCCCGC CCCATCAGCT TCCGACCGCA GTCCGGCGAC 
TACGCGGACG TGGACTTGGA GTACGATCGG TTCGGACGTC TGGTCAGTTG GAAGTGGGGT 
GTCCTGCAAG AAGCCTACTC TTTCGACCGC AATGGCCGTC TGAACGAGAT CAAGTACGGC 
GATGGCTCAA CAATGGTCTA CGCATTCAAG GACATGTTTG GCTCGTTACC GCTGAAGGTG 
ACTACGCCTA GACGTTCTGA TTATCTCCTG CAGTACGATG ATGCAGGTGC ACTCCAGAGC 
CTGACGACAC CTCGTGGACA CATTCACGCA TTCTCCCTGC AAACTTCTCT TGGCTTCTTT 
AAGTACCAAT ACTATTCACC AATCAACCGA CACCCCTTCG AGATTCTATA TAACGACGAA 
GGACAGATCT TGGCCAAAAT CCACCCCCAC CAATCTGGCA AGGTGGCATT CGTACATGAT 
ACTGCCGGAC GACTGGAGAC GATCCTCGCA GGATTGTCCA GCACCCACTA TACCTACCAG 
GATACAACTA GCCTGGTAAA GTCCGTGGAG GTGCAGGAGC CGGGCTTCGA GTTGCGCCGA 
GAGTTTAAAT ACCATGCAGG TATCCTAAAG GACGAGAAGT TGCGCTTCGG CTCCAAGAAC 
TCGCTGGCCT CGGCGCGCTA CAAGTACGCC TACGACGGAA ACGCCCGTCT CAGCGGCATC 
GAGATGGCCA TCGACGATAA GGAATTACCA ACCACGCGGT ACAAGTACAG CCAAAACTTG 
GGACAACTGG AGGTCGTGCA GGACCTGAAG ATCACGCGCA ACGCTTTTAA CCGAACAGTC 
ATACAGGACT CTGCCAAACA ATTCTTCGCC ATTGTGGACT ACGACCAGCA TGGACGGGTT 
AAGAGCGTGC TAATGAACGT GAAGAACATT GATGTGTTCC GCCTTGAGTT GGACTACGAT 
CTTCGCAACC GCATCAAGTC GCAAAAGACG ACCTTCGGTA GATCGACAGC CTTTGATAAG 
ATTAACTACA ATGCCGACGG ACATGTTGTT GAGGTCCTGG GCACCAACAA CTGGAAGTAT 
CTGTTCGACG AGAACGGCAA CACTGTTGGC GTGGTTGACC AGGGTGAGAA GTTCAACCTG 
GGCTACGACA TCGGAGACCG GGTGATCAAG GTAGGCGATG TGGAGTTCAA CAACTACGAT 
GCCCGCGGCT TCGTTGTGAA GCGCGGTGAG CAGAAGTACC GCTACAACAA CCGCGGACAA 
CTGATTCACT CGTTCGAAAG GGAACGTTTC CAGAGCTGGT ACTACTACGA CGACCGCAGC 
CGCCTCGTGG CCTGGCATGA TAACAAAGGT AACACCACGC AGTACTACTA CGCCAATCCT 
CGCACTCCTC ATCTCGTGAC CCACGTTCAC TTCCCGAAGA TCAGCCGCAC CATGAAGCTG 
TTCTACGATG ACCGCGACAT GCTAATCGCT TTGGAGCATG AGGACCAGCG CTACTACGTG 
GCCACCGACC AGAACGGCTC GCCACTAGCC TTCTTTGATC AGAATGGCAG CATTGTCAAG 
GAAATGAAGC GAACGCCCTT CGGCCGAATC ATTAAGGACA CTAAGCCGGA GTTCTTTGTG 
CCCATCGACT TCCACGGCGG ACTGATCGAC CCACACACAA AGCTGGTGTA CACCGAGCAG 
CGGCAGTACG ACCCGCACGT TGGCCAATGG ATGACCCCGT TGTGGGAGAC GCTCGCCACC 
GAGATGTCAC ACCCGACGGA TGTTTTTATA TACCGCTACC ACAACAACGA CCCCATCAAC 
CCGAACAAGC CCCAAAACTA TATGATCGAT CTGGATTCCT GGCTCCAGTT ATTCGGCTAC 
GATTTGAACA ACATGCAAAG CAGCCGCTAC ACCAAGCTGG CCCAGTATAC GCCGCAGGCT 
TCCATCAAGT CAAACACGTT GGCCCCCGAC TTTGGCGTGA TCTCCGGCCT GGAGTGCATC 
GTGGAAAAAA CAAGTGAGAA GTTCAGTGAT TTTGACTTTG TGCCCAAACC GCTGCTGAAA 
ATGGAGCCAA AGATGCGCAA CTTGCTCCCG CGTGTCAGCT ACCGGCGCGG TGTGTTCGGC 
GAAGGTGTAC TCCTCTCGAG AATCGGCGGA CGGGCGTTAG TTAGCGTGGT CGACGGATCA 
AACAGCGTGG TGCAGGACGT AGTGAGCAGC GTGTTCAACA ACTCTTACTT CCTTGACCTA 
CACTTCAGCA TTCACGACCA AGACGTATTT TACTTCGTAA AGGATAATGT CCTGAAGCTG 
CGCGACGACA ACGAGGAGCT GCGTCGCCTT GGCGGCATGT TCAACATATC AACCCATGAA 
ATCAGCGACC ACGGAGGCAG CGCCGCTAAG GAGCTGCGTC TTCACGGCCC AGACGCCGTT 
GTCATTATCA AATATGGCGT TGATCCCGAG CAGGAGCGCC ACCGCATCCT AAAGCATGCC 
CACAAGCGGG CAGTGGAGCG GGCTTGGGAG TTGGAGAAGC AGCTGGTGGC TGCCGGCTTC 
CAAGGCCGTG GCGACTGGAC CGAGGAGGAG AAAGAGGAGC TCGTCCAACA CGGTGACGTC 
GACGGCTGGA ACGGAATCGA TATCCACAGC ATACACAAGT ATCCGCAGCT GGCCGACGAC 
CCCGGTAACG TGGCTTTCCA GCGGGACGCG AAGCGTAAGC GACGCAAGAC CGGCAGCAGC 
CATCGGAGTG CCTCCAACCG CCGCCAGCTC AAGTTCGGCG AGCTGAGTGC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ten-m ( NM_001275282.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262211.1 Drosophila melanogaster tenascin major, transcript variant E (Ten-m), mRNA.

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CloneID	OFa20341
Clone ID Related Accession (Same CDS sequence)	NM_001275282.1
Accession Version	NM_001275282.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tenascin major, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATCCCT ACGAGTATGA GTCGACTCTA GACTGCCGCG ACGTGGGCGG CGGCCCCACT 
CCGGCTCACG CCCACCCACA CGCCCAAGGA CGTACGCTCC CCATGAGCGG CCACGGGCGG 
CCGACGACGG ACCTCGGTCC GGTCCACGGC AGCCAGACGC TCCAGCACCA AAACCAACAG 
AACCTGCAGG CCGCCCAAGC CGCCGCCCAG TCGTCCCACT ACGACTACGA GTACCAGCAC 
TTGGCCCACC GTCCGCCGGA CACGGCCAAC AACACAGCGC AGCGGACACA CGGAAGACAA 
GGCTTCCTCC TGGAAGGAGT CACGCCAACG GCACCACCGG ATGTTCCGCC GCGTAATCCG 
ACAATGAGTC GCATGCAAAA TGGTCGTTTA ACCGTTAACA ATCCCAATGA CGCTGACTTT 
GAGCCATCTT GTTTGGTGCG AACGCCATCG GGCAACGTTT ACATTCCTAG TGGAAATCTA 
AACATCAACA AGGGATCACC CATCGACTTC AAGAGCGGCT CGGCCTGCTC CACACCCACG 
AAGGATACGC TGAAGGGCTA CGAGCGGAGC ACGCAGGGCT GCATGGGTCC TGTGCTGCCG 
CAGCGCAGCG TCATGAACGG ACTGCCCGCA CACCACTACT CGGCGCCGAT GAACTTCCGC 
AAGGACCTGG TCGCGCGCTG CTCCTCGCCG TGGTTCGGTA TAGGATCCAT CTCGGTGCTC 
TTCGCCTTCG TGGTCATGCT TATCCTGCTA ACCACCACCG GCGTTATAAA ATGGAATCAG 
TCACCACCCT GCTCCGTTCT AGTCGGCAAC GAGGCCTCTG AGGTCACGGC TGCCAAGAGC 
ACGAACACGG ACCTCTCCAA GCTGCACAAT TCATCGGTGC GCGCGAAGAA CGGACAAGGA 
ATCGGACTGG CCCAGGGACA ATCGGGACTC GGAGCAGCAG GAGTGGGATC CGGCGGTGGA 
TCTTCCGCGG CCACTGTGAC GACGGCCACC TCGAACAGCG GCACCGCCCA AGGACTGCAG 
TCGACGTCGG CCTCCGCGGA GGCCACGTCC TCGGCGGCCA CGTCTTCCTC CCAATCCTCT 
CTAACGCCTT CGCTGTCCTC GTCCCTGGCG AATGCCAATA ATGGAGGACG AGCGATGTCA 
ACGCGGCTGT CAGTCCGTGG GGCAGGAGAA AGAGGACGTC GACATCGCCG AAGCCTAAAC 
GAGGAGCAGG GCGAAGATGA TGTGGCTACA GATGGAACTT TCAGTGACTT AATTACAAAC 
GAAAGCCTCA ACCAGCAGGC GGCTGAAAAG TATTTGGCAA CTACCCTAGC AAAAAGCCCG 
ACTGATGTTC ACGGCAGTGG CAATAAAACT TTACCCCGAA TGGATGGAGT ATACGGTACC 
CAAAGGAGCG AAGACACCCC GGACACCAGC TACGACTATG TCTATGAAGA CGAAGTTGAG 
CCGGAGACCA CCCCTTCGCT CATCCGTAGA ACCAAGACGG GGCAACAATT TGGCAAATCA 
TTAAACAGCA ATTTACGCAG CGCTGCTAAA ACACTGGTCA ACAAGAGGAG GAAATATGAC 
CACGGAACGG TGGAAGCGGA GCATATTAAG CATGAGGAGG AGGAGGAGGA GGATGAGCAG 
AAGCTGGAGC GCCACGAAGC GATCGGGATG GCAACGGAGC TGACGACGGA GTCGGAGACC 
AGCACATTGC CTGCTGTTAT TGATGACGAT AATCAAAGTG ACAACAGCAG CTCAGGCCCA 
ACGCCGGAGA CGACTGTAAG GAGCGACACC GATGACATTG TTGAAATCAA CACCCCCCCA 
TCACAGACAG CTCAAAGGAC TTTCGCCGCC GTCTCGCACC AGCCTGCCAT TGAACATGAT 
TTTCAAATCA AAGGGACCGA TGCTGGGGGC TTGCAGACGG AGAAACCTGC CACTGATGAT 
ATCAATAATG AGCGTGATTT AGCTGACAAC TATGAGGTAG ACAGTAAGGA GCCGACCTCG 
CCGGGCACAC CCCCACAAGG TAAGGTGAGT CAGCAAACTG GAAAAGCGTC ACTACAATCA 
CTTCAATCTG AATCGGATCT GATGATGAAT GATGCTTCGC ACTATGAGGA TATCGATATA 
GTAAAACTAG ATGGATTGAC GATCTCCCAC GAGGAGGAGA TTTACAAGAC GGCAGATAAG 
GAAAATATGG CACCAAAAAA TCAACCATCT CAGCATATAG ATAGAAGTCA AAACGAGGTA 
CTGAAAGGCC ATCAGCAGGG TGACGAAAAA CAACCCCAAC TAGAGCCCCT TAAACCGTAT 
GTGAGTGAGC GAGTTGATCT GCCGGGCAAA CGCATATTCC TGAATCTGAC TATAGCCACA 
GACGAGGGCA GTGACTCCGT TTACACCTTG CACGTCGAAG TGCCCACGGG CGGAGGACCC 
CACTTCATCA AGGAGGTCCT GACGCATGAG AAACCGACTG CCCAGGCAGA CAGTTGTGTT 
CCGGAACCAC CACCGCGCAT GCCTGACTGC CCGTGCAGTT GCCTCCCGCC TCCAGCGCCC 
ATCTATCTGG ATGACACCGT TGACATCGAC TCTGCCCCAC CAGCCAAAAC TGTTACGACG 
AGCACGATCT CGGCACCTAT TAACCCCTTC CATAGCGAGG AGGAGGATGA CGAAGACGGG 
GTTAGAGATG AGGAGCAAAC ACCTTCTTCA AGCACGGCTA CTAACCTCCC TAGCACTGAG 
ATCGATAACC ACATTGCCGC CTTTACTGAA CCCGCTGTTG GTGCTGGTGG TGTGCCGTTT 
GCATGCCCTG ATGTGATGCC AGTACTAATA CTGGAAGGAG CCCGAACATT CCCTGCACGC 
AGTTTCCCAC CGGACGGCAC TACCTTTGGC CAGATTACTC TCGGCCAGAA GCTGACCAAG 
GAGATCCAGC CGTACAGCTA CTGGAACATG CAGTTTTACC AGTCGGAACC TGCCTACGTT 
AAGTTCGACT ACACGATTCC GAGGGGCGCC TCCATCGGTG TATACGGCCG ACGCAACGCC 
CTACCCACCC ACACGCAGTA CCACTTTAAG GAAGTGCTCA GTGGATTTAG TGCCAGCACA 
CGAACGGCCC GGGCCGCTCA CCTGTCGATA ACTCGGGAGG TGACACGCTA TATGGAGCCG 
GGGCACTGGT TCGTCTCGCT TTACAACGAC GACGGGGACG TGCAGGAGCT GACCTTCTAC 
GCGGCCGTAG CCGAGGACAT GACCCAAAAC TGTCCCAACG GCTGCTCCGG CAACGGTCAG 
TGCCTGCTGG GTCACTGCCA GTGCAACCCC GGCTTCGGGG GCGACGACTG CAGCGAGAGC 
GTGTGCCCCG TACTGTGCTC CCAACATGGC GAGTACACCA ACGGAGAGTG CATCTGTAAC 
CCGGGCTGGA AGGGCAAGGA GTGCTCTCTG CGCCACGACG AGTGCGAGGT GGCCGACTGC 
AGTGGGCACG GCCACTGTGT CAGTGGAAAA TGCCAATGCA TGCGCGGCTA CAAAGGCAAA 
TTCTGCGAAG AAGTTGACTG CCCACATCCG AACTGCTCAG GCCACGGATT CTGCGCCGAT 
GGCACTTGCA TATGCAAGAA GGGCTGGAAG GGACCTGACT GCGCTACCAT GGACCAGGAT 
GCGCTGCAGT GCCTCCCCGA CTGTTCCGGC CACGGCACCT TCGACCTGGA CACACAGACC 
TGTACCTGCG AGGCAAAGTG GAGTGGGGAC GATTGTTCCA AGGAGCTGTG CGACTTGGAC 
TGCGGTCAGC ACGGACGTTG TGAAGGTGAC GCTTGTGCAT GCGACCCGGA GTGGGGTGGC 
GAGTATTGCA ACACCCGGTT GTGCGACGTC CGCTGTAACG AGCATGGCCA GTGCAAGAAT 
GGTACTTGTC TGTGCGTTAC TGGATGGAAC GGAAAGCACT GCACCATCGA GGGCTGTCCC 
AATAGCTGCG CCGGGCATGG CCAGTGCCGT GTAAGTGGCG AGGGTCAATG GGAGTGTCGC 
TGCTACGAGG GCTGGGACGG GCCGGACTGT GGGATTGCAC TGGAGTTGAA CTGTGGCGAC 
AGCAAGGACA ACGACAAGGA TGGCTTGGTT GATTGCGAGG ATCCTGAGTG CTGTGCCAGC 
CACGTATGCA AGACCTCCCA ACTATGTGTT TCAGCTCCGA AGCCCATTGA TGTGCTGCTC 
AGAAAACAGC CGCCAGCTAT TACGGCCTCT TTCTTTGAGC GGATGAAGTT CCTCATCGAC 
GAGAGCAGCC TGCAGAACTA CGCCAAACTG GAAACCTTTA ACGAAAGCAT TTTTTGGAAT 
TATTTCAATG CAAGTCGATC AGCGGTGATC AGAGGCCGCG TTGTTACATC TCTGGGCATG 
GGACTGGTGG GTGTGCGAGT GTCCACCACC ACGCTCCTAG AGGGCTTCAC CCTGACCCGA 
GATGATGGAT GGTTCGACCT AATGGTTAAC GGTGGTGGAG CCGTAACGCT GCAGTTCGGA 
CGCGCACCTT TCCGGCCGCA GTCGCGCATC GTGCAAGTTC CATGGAACGA GGTCGTGATT 
ATTGACTTGG TCGTCATGAG TATGTCCGAG GAAAAGGGAT TGGCCGTAAC TACGACACAC 
ACATGCTTTG CACACGACTA CGACCTGATG AAGCCCGTGG TGCTGGCTTC GTGGAAGCAC 
GGGTTTCAGG GAGCCTGTCC CGATCGCAGC GCCATTCTTG CTGAATCTCA AGTGATTCAG 
GAGTCGTTGC AGATCCCGGG TACAGGCCTC AACCTGGTTT ACCACTCATC GCGTGCTGCC 
GGCTACCTTT CCACTATTAA GCTGCAGTTA ACTCCGGATG TGATTCCGAC ATCGCTACAT 
CTTATCCATC TCCGCATAAC AATCGAGGGA ATACTCTTTG AACGAATATT CGAAGCAGAT 
CCGGGAATTA AGTTCACGTA CGCCTGGAAC CGACTCAACA TCTACCGGCA ACGAGTCTAC 
GGTGTAACCA CGGCCGTGGT GAAGGTTGGC TACCAGTACA CCGACTGCAC TGACATTGTG 
TGGGACATTC AAACAACTAA GCTAAGCGGT CACGACATGT CGATCTCAGA AGTGGGTGGG 
TGGAACCTGG ACATCCATCA CCGCTACAAC TTCCACGAGG GAATTCTGCA AAAGGGCGAC 
GGCTCAAACA TATATCTGCG CAATAAGCCG CGAATCATCC TGACCACAAT GGGAGATGGC 
CACCAGCGGC CTCTGGAGTG TCCCGATTGC GACGGCCAGG CGACAAAGCA GCGACTTCTG 
GCACCCGTCG CACTGGCCGC CGCTCCTGAC GGCAGTCTTT TCGTCGGTGA TTTTAATTAC 
ATCCGCCGCA TCATGACCGA CGGCAGCATC CGCACTGTGG TCAAGCTGAA CGCGACTCGC 
GTCTCGTACC GCTACCACAT GGCCCTCAGC CCGCTCGACG GAACTCTTTA TGTTTCGGAT 
CCAGAATCAC ACCAAATCAT TCGGGTACGC GACACCAACG ACTACTCGCA ACCGGAACTA 
AACTGGGAAG CGGTTGTGGG CTCCGGGGAG CGCTGTCTTC CTGGTGACGA AGCGCACTGC 
GGCGATGGGG CACTGGCAAA GGATGCCAAA TTGGCTTATC CGAAAGGCAT TGCGATTTCG 
AGCGACAACA TCTTGTACTT TGCAGACGGA ACTAATATCC GTATGGTGGA CCGGGATGGA 
ATTGTAAGCA CTCTGATCGG CAACCACATG CACAAGTCCC ACTGGAAGCC AATTCCCTGC 
GAGGGAACCC TGAAGCTGGA GGAGATGCAT CTTCGCTGGC CCACTGAGCT AGCAGTCTCC 
CCGATGGACA ACACGCTGCA CATAATCGAC GACCACATGA TCCTGCGGAT GACACCAGAC 
GGTCGTGTGC GCGTCATCTC CGGCCGTCCC CTACATTGCG CCACAGCCTC CACTGCCTAC 
GATACGGATC TGGCTACCCA CGCTACCCTG GTGATGCCGC AGTCTATCGC GTTCGGTCCA 
CTCGGTGAGC TTTACGTGGC GGAAAGCGAC TCTCAACGAA TAAACCGGGT GCGCGTGATC 
GGCACGGACG GAAGGATCGC TCCGTTTGCT GGTGCCGAAT CCAAGTGCAA TTGCCTGGAG 
CGCGGTTGCG ACTGCTTCGA AGCGGAACAC TATCTGGCCA CCAGTGCCAA GTTCAACACG 
ATCGCCGCCC TTGCTGTCAC ACCCGATAGC CATGTACACA TCGCGGACCA GGCCAACTAC 
CGTATCCGCT CCGTTATGTC GAGCATCCCA GAGGCAAGTC CGTCGCGCGA GTACGAGATC 
TACGCACCTG ACATGCAGGA GATATACATT TTCAACCGAT TCGGACAGCA CGTGTCCACA 
CGCAACATCC TGACCGGAGA GACGACCTAT GTGTTTACCT ACAACGTAAA CACCTCCAAC 
GGAAAACTGA GCACCGTAAC AGACGCGGCT GGCAACAAAG TGTTCCTGCT TCGAGATTAC 
ACTTCTCAGG TAAATTCCAT CGAGAACACG AAGGGTCAAA AGTGCCGCCT GCGCATGACC 
AGGATGAAAA TGCTGCACGA GCTGAGCACT CCAGACAACT ACAATGTGAC CTACGAATAT 
CACGGCCCCA CCGGTCTGCT ACGAACCAAG CTGGACTCCA CCGGACGATC CTACGTTTAC 
AACTACGATG AGTTTGGTCG CCTCACTTCT GCAGTGACTC CCACCGGTCG TGTCATCGAG 
CTGAGCTTCG ACCTGAGCGT GAAGGGAGCC CAGGTAAAGG TTTCGGAGAA CGCCCAGAAG 
GAAATGTCAC TGCTGATCCA AGGTGCCACC GTTATTGTGC GCAACGGAGC CGCCGAGTCG 
CGTACCACCG TTGACATGGA CGGCTCCACC ACAAGCATCA CTCCATGGGG CCACAACCTG 
CAGATGGAAG TGGCACCTTA TACCATTCTG GCAGAACAAA GTCCACTGCT TGGCGAGAGC 
TACCCGGTCC CGGCTAAGCA ACGCACTGAG ATTGCTGGTG ACTTGGCCAA CAGGTTTGAG 
TGGCGCTACT TTGTGCGCCG GCAGCAGCCG CTGCAAGCGG GAAAACAGAG CAAGGGACCG 
CCACGTCCCG TAACCGAAGT TGGACGTAAG CTGCGTGTGA ACGGAGACAA CGTGCTGACT 
TTGGAGTACG ACCGCGAGAC CCAGTCGGTG GTCGTTATGG TGGACGACAA GCAGGAGCTG 
CTTAACGTGA CCTACGATCG CACTTCCCGC CCCATCAGCT TCCGACCGCA GTCCGGCGAC 
TACGCGGACG TGGACTTGGA GTACGATCGG TTCGGACGTC TGGTCAGTTG GAAGTGGGGT 
GTCCTGCAAG AAGCCTACTC TTTCGACCGC AATGGCCGTC TGAACGAGAT CAAGTACGGC 
GATGGCTCAA CAATGGTCTA CGCATTCAAG GACATGTTTG GCTCGTTACC GCTGAAGGTG 
ACTACGCCTA GACGTTCTGA TTATCTCCTG CAGTACGATG ATGCAGGTGC ACTCCAGAGC 
CTGACGACAC CTCGTGGACA CATTCACGCA TTCTCCCTGC AAACTTCTCT TGGCTTCTTT 
AAGTACCAAT ACTATTCACC AATCAACCGA CACCCCTTCG AGATTCTATA TAACGACGAA 
GGACAGATCT TGGCCAAAAT CCACCCCCAC CAATCTGGCA AGGTGGCATT CGTACATGAT 
ACTGCCGGAC GACTGGAGAC GATCCTCGCA GGATTGTCCA GCACCCACTA TACCTACCAG 
GATACAACTA GCCTGGTAAA GTCCGTGGAG GTGCAGGAGC CGGGCTTCGA GTTGCGCCGA 
GAGTTTAAAT ACCATGCAGG TATCCTAAAG GACGAGAAGT TGCGCTTCGG CTCCAAGAAC 
TCGCTGGCCT CGGCGCGCTA CAAGTACGCC TACGACGGAA ACGCCCGTCT CAGCGGCATC 
GAGATGGCCA TCGACGATAA GGAATTACCA ACCACGCGGT ACAAGTACAG CCAAAACTTG 
GGACAACTGG AGGTCGTGCA GGACCTGAAG ATCACGCGCA ACGCTTTTAA CCGAACAGTC 
ATACAGGACT CTGCCAAACA ATTCTTCGCC ATTGTGGACT ACGACCAGCA TGGACGGGTT 
AAGAGCGTGC TAATGAACGT GAAGAACATT GATGTGTTCC GCCTTGAGTT GGACTACGAT 
CTTCGCAACC GCATCAAGTC GCAAAAGACG ACCTTCGGTA GATCGACAGC CTTTGATAAG 
ATTAACTACA ATGCCGACGG ACATGTTGTT GAGGTCCTGG GCACCAACAA CTGGAAGTAT 
CTGTTCGACG AGAACGGCAA CACTGTTGGC GTGGTTGACC AGGGTGAGAA GTTCAACCTG 
GGCTACGACA TCGGAGACCG GGTGATCAAG GTAGGCGATG TGGAGTTCAA CAACTACGAT 
GCCCGCGGCT TCGTTGTGAA GCGCGGTGAG CAGAAGTACC GCTACAACAA CCGCGGACAA 
CTGATTCACT CGTTCGAAAG GGAACGTTTC CAGAGCTGGT ACTACTACGA CGACCGCAGC 
CGCCTCGTGG CCTGGCATGA TAACAAAGGT AACACCACGC AGTACTACTA CGCCAATCCT 
CGCACTCCTC ATCTCGTGAC CCACGTTCAC TTCCCGAAGA TCAGCCGCAC CATGAAGCTG 
TTCTACGATG ACCGCGACAT GCTAATCGCT TTGGAGCATG AGGACCAGCG CTACTACGTG 
GCCACCGACC AGAACGGCTC GCCACTAGCC TTCTTTGATC AGAATGGCAG CATTGTCAAG 
GAAATGAAGC GAACGCCCTT CGGCCGAATC ATTAAGGACA CTAAGCCGGA GTTCTTTGTG 
CCCATCGACT TCCACGGCGG ACTGATCGAC CCACACACAA AGCTGGTGTA CACCGAGCAG 
CGGCAGTACG ACCCGCACGT TGGCCAATGG ATGACCCCGT TGTGGGAGAC GCTCGCCACC 
GAGATGTCAC ACCCGACGGA TGTTTTTATA TACCGCTACC ACAACAACGA CCCCATCAAC 
CCGAACAAGC CCCAAAACTA TATGATCGAT CTGGATTCCT GGCTCCAGTT ATTCGGCTAC 
GATTTGAACA ACATGCAAAG CAGCCGCTAC ACCAAGCTGG CCCAGTATAC GCCGCAGGCT 
TCCATCAAGT CAAACACGTT GGCCCCCGAC TTTGGCGTGA TCTCCGGCCT GGAGTGCATC 
GTGGAAAAAA CAAGTGAGAA GTTCAGTGAT TTTGACTTTG TGCCCAAACC GCTGCTGAAA 
ATGGAGCCAA AGATGCGCAA CTTGCTCCCG CGTGTCAGCT ACCGGCGCGG TGTGTTCGGC 
GAAGGTGTAC TCCTCTCGAG AATCGGCGGA CGGGCGTTAG TTAGCGTGGT CGACGGATCA 
AACAGCGTGG TGCAGGACGT AGTGAGCAGC GTGTTCAACA ACTCTTACTT CCTTGACCTA 
CACTTCAGCA TTCACGACCA AGACGTATTT TACTTCGTAA AGGATAATGT CCTGAAGCTG 
CGCGACGACA ACGAGGAGCT GCGTCGCCTT GGCGGCATGT TCAACATATC AACCCATGAA 
ATCAGCGACC ACGGAGGCAG CGCCGCTAAG GAGCTGCGTC TTCACGGCCC AGACGCCGTT 
GTCATTATCA AATATGGCGT TGATCCCGAG CAGGAGCGCC ACCGCATCCT AAAGCATGCC 
CACAAGCGGG CAGTGGAGCG GGCTTGGGAG TTGGAGAAGC AGCTGGTGGC TGCCGGCTTC 
CAAGGCCGTG GCGACTGGAC CGAGGAGGAG AAAGAGGAGC TCGTCCAACA CGGTGACGTC 
GACGGCTGGA ACGGAATCGA TATCCACAGC ATACACAAGT ATCCGCAGCT GGCCGACGAC 
CCCGGTAACG TGGCTTTCCA GCGGGACGCG AAGCGTAAGC GACGCAAGAC CGGCAGCAGC 
CATCGGAGTG CCTCCAACCG CCGCCAGCTC AAGTTCGGCG AGCTGAGTGC GTGAGTGGAG 
GCATATAGAG AGAAGCTGGT GGCGGAGACG AAGCCAGAGG AGAGTGACAT GACCGACGAA 
GAATACGGTG ACCAGGAGGA CTACCTAATT GCCGTGGGCA AAATGGAGCC AAGGGACAGC 
AGTGAGGCAC TTGACGAGCA GATTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262211.1
CDS	672..10721
Translation	MNPYEYESTLDCRDVGGGPTPAHAHPHAQGRTLPMSGHGRPTTDLGPVHGSQTLQHQNQQNLQAAQAAAQSSHYDYEYQHLAHRPPDTANNTAQRTHGRQGFLLEGVTPTAPPDVPPRNPTMSRMQNGRLTVNNPNDADFEPSCLVRTPSGNVYIPSGNLNINKGSPIDFKSGSACSTPTKDTLKGYERSTQGCMGPVLPQRSVMNGLPAHHYSAPMNFRKDLVARCSSPWFGIGSISVLFAFVVMLILLTTTGVIKWNQSPPCSVLVGNEASEVTAAKSTNTDLSKLHNSSVRAKNGQGIGLAQGQSGLGAAGVGSGGGSSAATVTTATSNSGTAQGLQSTSASAEATSSAATSSSQSSLTPSLSSSLANANNGGRAMSTRLSVRGAGERGRRHRRSLNEEQGEDDVATDGTFSDLITNESLNQQAAEKYLATTLAKSPTDVHGSGNKTLPRMDGVYGTQRSEDTPDTSYDYVYEDEVEPETTPSLIRRTKTGQQFGKSLNSNLRSAAKTLVNKRRKYDHGTVEAEHIKHEEEEEEDEQKLERHEAIGMATELTTESETSTLPAVIDDDNQSDNSSSGPTPETTVRSDTDDIVEINTPPSQTAQRTFAAVSHQPAIEHDFQIKGTDAGGLQTEKPATDDINNERDLADNYEVDSKEPTSPGTPPQGKVSQQTGKASLQSLQSESDLMMNDASHYEDIDIVKLDGLTISHEEEIYKTADKENMAPKNQPSQHIDRSQNEVLKGHQQGDEKQPQLEPLKPYVSERVDLPGKRIFLNLTIATDEGSDSVYTLHVEVPTGGGPHFIKEVLTHEKPTAQADSCVPEPPPRMPDCPCSCLPPPAPIYLDDTVDIDSAPPAKTVTTSTISAPINPFHSEEEDDEDGVRDEEQTPSSSTATNLPSTEIDNHIAAFTEPAVGAGGVPFACPDVMPVLILEGARTFPARSFPPDGTTFGQITLGQKLTKEIQPYSYWNMQFYQSEPAYVKFDYTIPRGASIGVYGRRNALPTHTQYHFKEVLSGFSASTRTARAAHLSITREVTRYMEPGHWFVSLYNDDGDVQELTFYAAVAEDMTQNCPNGCSGNGQCLLGHCQCNPGFGGDDCSESVCPVLCSQHGEYTNGECICNPGWKGKECSLRHDECEVADCSGHGHCVSGKCQCMRGYKGKFCEEVDCPHPNCSGHGFCADGTCICKKGWKGPDCATMDQDALQCLPDCSGHGTFDLDTQTCTCEAKWSGDDCSKELCDLDCGQHGRCEGDACACDPEWGGEYCNTRLCDVRCNEHGQCKNGTCLCVTGWNGKHCTIEGCPNSCAGHGQCRVSGEGQWECRCYEGWDGPDCGIALELNCGDSKDNDKDGLVDCEDPECCASHVCKTSQLCVSAPKPIDVLLRKQPPAITASFFERMKFLIDESSLQNYAKLETFNESIFWNYFNASRSAVIRGRVVTSLGMGLVGVRVSTTTLLEGFTLTRDDGWFDLMVNGGGAVTLQFGRAPFRPQSRIVQVPWNEVVIIDLVVMSMSEEKGLAVTTTHTCFAHDYDLMKPVVLASWKHGFQGACPDRSAILAESQVIQESLQIPGTGLNLVYHSSRAAGYLSTIKLQLTPDVIPTSLHLIHLRITIEGILFERIFEADPGIKFTYAWNRLNIYRQRVYGVTTAVVKVGYQYTDCTDIVWDIQTTKLSGHDMSISEVGGWNLDIHHRYNFHEGILQKGDGSNIYLRNKPRIILTTMGDGHQRPLECPDCDGQATKQRLLAPVALAAAPDGSLFVGDFNYIRRIMTDGSIRTVVKLNATRVSYRYHMALSPLDGTLYVSDPESHQIIRVRDTNDYSQPELNWEAVVGSGERCLPGDEAHCGDGALAKDAKLAYPKGIAISSDNILYFADGTNIRMVDRDGIVSTLIGNHMHKSHWKPIPCEGTLKLEEMHLRWPTELAVSPMDNTLHIIDDHMILRMTPDGRVRVISGRPLHCATASTAYDTDLATHATLVMPQSIAFGPLGELYVAESDSQRINRVRVIGTDGRIAPFAGAESKCNCLERGCDCFEAEHYLATSAKFNTIAALAVTPDSHVHIADQANYRIRSVMSSIPEASPSREYEIYAPDMQEIYIFNRFGQHVSTRNILTGETTYVFTYNVNTSNGKLSTVTDAAGNKVFLLRDYTSQVNSIENTKGQKCRLRMTRMKMLHELSTPDNYNVTYEYHGPTGLLRTKLDSTGRSYVYNYDEFGRLTSAVTPTGRVIELSFDLSVKGAQVKVSENAQKEMSLLIQGATVIVRNGAAESRTTVDMDGSTTSITPWGHNLQMEVAPYTILAEQSPLLGESYPVPAKQRTEIAGDLANRFEWRYFVRRQQPLQAGKQSKGPPRPVTEVGRKLRVNGDNVLTLEYDRETQSVVVMVDDKQELLNVTYDRTSRPISFRPQSGDYADVDLEYDRFGRLVSWKWGVLQEAYSFDRNGRLNEIKYGDGSTMVYAFKDMFGSLPLKVTTPRRSDYLLQYDDAGALQSLTTPRGHIHAFSLQTSLGFFKYQYYSPINRHPFEILYNDEGQILAKIHPHQSGKVAFVHDTAGRLETILAGLSSTHYTYQDTTSLVKSVEVQEPGFELRREFKYHAGILKDEKLRFGSKNSLASARYKYAYDGNARLSGIEMAIDDKELPTTRYKYSQNLGQLEVVQDLKITRNAFNRTVIQDSAKQFFAIVDYDQHGRVKSVLMNVKNIDVFRLELDYDLRNRIKSQKTTFGRSTAFDKINYNADGHVVEVLGTNNWKYLFDENGNTVGVVDQGEKFNLGYDIGDRVIKVGDVEFNNYDARGFVVKRGEQKYRYNNRGQLIHSFERERFQSWYYYDDRSRLVAWHDNKGNTTQYYYANPRTPHLVTHVHFPKISRTMKLFYDDRDMLIALEHEDQRYYVATDQNGSPLAFFDQNGSIVKEMKRTPFGRIIKDTKPEFFVPIDFHGGLIDPHTKLVYTEQRQYDPHVGQWMTPLWETLATEMSHPTDVFIYRYHNNDPINPNKPQNYMIDLDSWLQLFGYDLNNMQSSRYTKLAQYTPQASIKSNTLAPDFGVISGLECIVEKTSEKFSDFDFVPKPLLKMEPKMRNLLPRVSYRRGVFGEGVLLSRIGGRALVSVVDGSNSVVQDVVSSVFNNSYFLDLHFSIHDQDVFYFVKDNVLKLRDDNEELRRLGGMFNISTHEISDHGGSAAKELRLHGPDAVVIIKYGVDPEQERHRILKHAHKRAVERAWELEKQLVAAGFQGRGDWTEEEKEELVQHGDVDGWNGIDIHSIHKYPQLADDPGNVAFQRDAKRKRRKTGSSHRSASNRRQLKFGELSA*VEAYREKLVAETKPEESDMTDEEYGDQEDYLIAVGKMEPRDSSEALDEQIL

Target ORF information:

RefSeq Version	NM_001275282.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tenascin major, transcript variant E (Ten-m), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275282.1

ORF Insert Sequence:

ATGAATCCCT ACGAGTATGA GTCGACTCTA GACTGCCGCG ACGTGGGCGG CGGCCCCACT 
CCGGCTCACG CCCACCCACA CGCCCAAGGA CGTACGCTCC CCATGAGCGG CCACGGGCGG 
CCGACGACGG ACCTCGGTCC GGTCCACGGC AGCCAGACGC TCCAGCACCA AAACCAACAG 
AACCTGCAGG CCGCCCAAGC CGCCGCCCAG TCGTCCCACT ACGACTACGA GTACCAGCAC 
TTGGCCCACC GTCCGCCGGA CACGGCCAAC AACACAGCGC AGCGGACACA CGGAAGACAA 
GGCTTCCTCC TGGAAGGAGT CACGCCAACG GCACCACCGG ATGTTCCGCC GCGTAATCCG 
ACAATGAGTC GCATGCAAAA TGGTCGTTTA ACCGTTAACA ATCCCAATGA CGCTGACTTT 
GAGCCATCTT GTTTGGTGCG AACGCCATCG GGCAACGTTT ACATTCCTAG TGGAAATCTA 
AACATCAACA AGGGATCACC CATCGACTTC AAGAGCGGCT CGGCCTGCTC CACACCCACG 
AAGGATACGC TGAAGGGCTA CGAGCGGAGC ACGCAGGGCT GCATGGGTCC TGTGCTGCCG 
CAGCGCAGCG TCATGAACGG ACTGCCCGCA CACCACTACT CGGCGCCGAT GAACTTCCGC 
AAGGACCTGG TCGCGCGCTG CTCCTCGCCG TGGTTCGGTA TAGGATCCAT CTCGGTGCTC 
TTCGCCTTCG TGGTCATGCT TATCCTGCTA ACCACCACCG GCGTTATAAA ATGGAATCAG 
TCACCACCCT GCTCCGTTCT AGTCGGCAAC GAGGCCTCTG AGGTCACGGC TGCCAAGAGC 
ACGAACACGG ACCTCTCCAA GCTGCACAAT TCATCGGTGC GCGCGAAGAA CGGACAAGGA 
ATCGGACTGG CCCAGGGACA ATCGGGACTC GGAGCAGCAG GAGTGGGATC CGGCGGTGGA 
TCTTCCGCGG CCACTGTGAC GACGGCCACC TCGAACAGCG GCACCGCCCA AGGACTGCAG 
TCGACGTCGG CCTCCGCGGA GGCCACGTCC TCGGCGGCCA CGTCTTCCTC CCAATCCTCT 
CTAACGCCTT CGCTGTCCTC GTCCCTGGCG AATGCCAATA ATGGAGGACG AGCGATGTCA 
ACGCGGCTGT CAGTCCGTGG GGCAGGAGAA AGAGGACGTC GACATCGCCG AAGCCTAAAC 
GAGGAGCAGG GCGAAGATGA TGTGGCTACA GATGGAACTT TCAGTGACTT AATTACAAAC 
GAAAGCCTCA ACCAGCAGGC GGCTGAAAAG TATTTGGCAA CTACCCTAGC AAAAAGCCCG 
ACTGATGTTC ACGGCAGTGG CAATAAAACT TTACCCCGAA TGGATGGAGT ATACGGTACC 
CAAAGGAGCG AAGACACCCC GGACACCAGC TACGACTATG TCTATGAAGA CGAAGTTGAG 
CCGGAGACCA CCCCTTCGCT CATCCGTAGA ACCAAGACGG GGCAACAATT TGGCAAATCA 
TTAAACAGCA ATTTACGCAG CGCTGCTAAA ACACTGGTCA ACAAGAGGAG GAAATATGAC 
CACGGAACGG TGGAAGCGGA GCATATTAAG CATGAGGAGG AGGAGGAGGA GGATGAGCAG 
AAGCTGGAGC GCCACGAAGC GATCGGGATG GCAACGGAGC TGACGACGGA GTCGGAGACC 
AGCACATTGC CTGCTGTTAT TGATGACGAT AATCAAAGTG ACAACAGCAG CTCAGGCCCA 
ACGCCGGAGA CGACTGTAAG GAGCGACACC GATGACATTG TTGAAATCAA CACCCCCCCA 
TCACAGACAG CTCAAAGGAC TTTCGCCGCC GTCTCGCACC AGCCTGCCAT TGAACATGAT 
TTTCAAATCA AAGGGACCGA TGCTGGGGGC TTGCAGACGG AGAAACCTGC CACTGATGAT 
ATCAATAATG AGCGTGATTT AGCTGACAAC TATGAGGTAG ACAGTAAGGA GCCGACCTCG 
CCGGGCACAC CCCCACAAGG TAAGGTGAGT CAGCAAACTG GAAAAGCGTC ACTACAATCA 
CTTCAATCTG AATCGGATCT GATGATGAAT GATGCTTCGC ACTATGAGGA TATCGATATA 
GTAAAACTAG ATGGATTGAC GATCTCCCAC GAGGAGGAGA TTTACAAGAC GGCAGATAAG 
GAAAATATGG CACCAAAAAA TCAACCATCT CAGCATATAG ATAGAAGTCA AAACGAGGTA 
CTGAAAGGCC ATCAGCAGGG TGACGAAAAA CAACCCCAAC TAGAGCCCCT TAAACCGTAT 
GTGAGTGAGC GAGTTGATCT GCCGGGCAAA CGCATATTCC TGAATCTGAC TATAGCCACA 
GACGAGGGCA GTGACTCCGT TTACACCTTG CACGTCGAAG TGCCCACGGG CGGAGGACCC 
CACTTCATCA AGGAGGTCCT GACGCATGAG AAACCGACTG CCCAGGCAGA CAGTTGTGTT 
CCGGAACCAC CACCGCGCAT GCCTGACTGC CCGTGCAGTT GCCTCCCGCC TCCAGCGCCC 
ATCTATCTGG ATGACACCGT TGACATCGAC TCTGCCCCAC CAGCCAAAAC TGTTACGACG 
AGCACGATCT CGGCACCTAT TAACCCCTTC CATAGCGAGG AGGAGGATGA CGAAGACGGG 
GTTAGAGATG AGGAGCAAAC ACCTTCTTCA AGCACGGCTA CTAACCTCCC TAGCACTGAG 
ATCGATAACC ACATTGCCGC CTTTACTGAA CCCGCTGTTG GTGCTGGTGG TGTGCCGTTT 
GCATGCCCTG ATGTGATGCC AGTACTAATA CTGGAAGGAG CCCGAACATT CCCTGCACGC 
AGTTTCCCAC CGGACGGCAC TACCTTTGGC CAGATTACTC TCGGCCAGAA GCTGACCAAG 
GAGATCCAGC CGTACAGCTA CTGGAACATG CAGTTTTACC AGTCGGAACC TGCCTACGTT 
AAGTTCGACT ACACGATTCC GAGGGGCGCC TCCATCGGTG TATACGGCCG ACGCAACGCC 
CTACCCACCC ACACGCAGTA CCACTTTAAG GAAGTGCTCA GTGGATTTAG TGCCAGCACA 
CGAACGGCCC GGGCCGCTCA CCTGTCGATA ACTCGGGAGG TGACACGCTA TATGGAGCCG 
GGGCACTGGT TCGTCTCGCT TTACAACGAC GACGGGGACG TGCAGGAGCT GACCTTCTAC 
GCGGCCGTAG CCGAGGACAT GACCCAAAAC TGTCCCAACG GCTGCTCCGG CAACGGTCAG 
TGCCTGCTGG GTCACTGCCA GTGCAACCCC GGCTTCGGGG GCGACGACTG CAGCGAGAGC 
GTGTGCCCCG TACTGTGCTC CCAACATGGC GAGTACACCA ACGGAGAGTG CATCTGTAAC 
CCGGGCTGGA AGGGCAAGGA GTGCTCTCTG CGCCACGACG AGTGCGAGGT GGCCGACTGC 
AGTGGGCACG GCCACTGTGT CAGTGGAAAA TGCCAATGCA TGCGCGGCTA CAAAGGCAAA 
TTCTGCGAAG AAGTTGACTG CCCACATCCG AACTGCTCAG GCCACGGATT CTGCGCCGAT 
GGCACTTGCA TATGCAAGAA GGGCTGGAAG GGACCTGACT GCGCTACCAT GGACCAGGAT 
GCGCTGCAGT GCCTCCCCGA CTGTTCCGGC CACGGCACCT TCGACCTGGA CACACAGACC 
TGTACCTGCG AGGCAAAGTG GAGTGGGGAC GATTGTTCCA AGGAGCTGTG CGACTTGGAC 
TGCGGTCAGC ACGGACGTTG TGAAGGTGAC GCTTGTGCAT GCGACCCGGA GTGGGGTGGC 
GAGTATTGCA ACACCCGGTT GTGCGACGTC CGCTGTAACG AGCATGGCCA GTGCAAGAAT 
GGTACTTGTC TGTGCGTTAC TGGATGGAAC GGAAAGCACT GCACCATCGA GGGCTGTCCC 
AATAGCTGCG CCGGGCATGG CCAGTGCCGT GTAAGTGGCG AGGGTCAATG GGAGTGTCGC 
TGCTACGAGG GCTGGGACGG GCCGGACTGT GGGATTGCAC TGGAGTTGAA CTGTGGCGAC 
AGCAAGGACA ACGACAAGGA TGGCTTGGTT GATTGCGAGG ATCCTGAGTG CTGTGCCAGC 
CACGTATGCA AGACCTCCCA ACTATGTGTT TCAGCTCCGA AGCCCATTGA TGTGCTGCTC 
AGAAAACAGC CGCCAGCTAT TACGGCCTCT TTCTTTGAGC GGATGAAGTT CCTCATCGAC 
GAGAGCAGCC TGCAGAACTA CGCCAAACTG GAAACCTTTA ACGAAAGCAT TTTTTGGAAT 
TATTTCAATG CAAGTCGATC AGCGGTGATC AGAGGCCGCG TTGTTACATC TCTGGGCATG 
GGACTGGTGG GTGTGCGAGT GTCCACCACC ACGCTCCTAG AGGGCTTCAC CCTGACCCGA 
GATGATGGAT GGTTCGACCT AATGGTTAAC GGTGGTGGAG CCGTAACGCT GCAGTTCGGA 
CGCGCACCTT TCCGGCCGCA GTCGCGCATC GTGCAAGTTC CATGGAACGA GGTCGTGATT 
ATTGACTTGG TCGTCATGAG TATGTCCGAG GAAAAGGGAT TGGCCGTAAC TACGACACAC 
ACATGCTTTG CACACGACTA CGACCTGATG AAGCCCGTGG TGCTGGCTTC GTGGAAGCAC 
GGGTTTCAGG GAGCCTGTCC CGATCGCAGC GCCATTCTTG CTGAATCTCA AGTGATTCAG 
GAGTCGTTGC AGATCCCGGG TACAGGCCTC AACCTGGTTT ACCACTCATC GCGTGCTGCC 
GGCTACCTTT CCACTATTAA GCTGCAGTTA ACTCCGGATG TGATTCCGAC ATCGCTACAT 
CTTATCCATC TCCGCATAAC AATCGAGGGA ATACTCTTTG AACGAATATT CGAAGCAGAT 
CCGGGAATTA AGTTCACGTA CGCCTGGAAC CGACTCAACA TCTACCGGCA ACGAGTCTAC 
GGTGTAACCA CGGCCGTGGT GAAGGTTGGC TACCAGTACA CCGACTGCAC TGACATTGTG 
TGGGACATTC AAACAACTAA GCTAAGCGGT CACGACATGT CGATCTCAGA AGTGGGTGGG 
TGGAACCTGG ACATCCATCA CCGCTACAAC TTCCACGAGG GAATTCTGCA AAAGGGCGAC 
GGCTCAAACA TATATCTGCG CAATAAGCCG CGAATCATCC TGACCACAAT GGGAGATGGC 
CACCAGCGGC CTCTGGAGTG TCCCGATTGC GACGGCCAGG CGACAAAGCA GCGACTTCTG 
GCACCCGTCG CACTGGCCGC CGCTCCTGAC GGCAGTCTTT TCGTCGGTGA TTTTAATTAC 
ATCCGCCGCA TCATGACCGA CGGCAGCATC CGCACTGTGG TCAAGCTGAA CGCGACTCGC 
GTCTCGTACC GCTACCACAT GGCCCTCAGC CCGCTCGACG GAACTCTTTA TGTTTCGGAT 
CCAGAATCAC ACCAAATCAT TCGGGTACGC GACACCAACG ACTACTCGCA ACCGGAACTA 
AACTGGGAAG CGGTTGTGGG CTCCGGGGAG CGCTGTCTTC CTGGTGACGA AGCGCACTGC 
GGCGATGGGG CACTGGCAAA GGATGCCAAA TTGGCTTATC CGAAAGGCAT TGCGATTTCG 
AGCGACAACA TCTTGTACTT TGCAGACGGA ACTAATATCC GTATGGTGGA CCGGGATGGA 
ATTGTAAGCA CTCTGATCGG CAACCACATG CACAAGTCCC ACTGGAAGCC AATTCCCTGC 
GAGGGAACCC TGAAGCTGGA GGAGATGCAT CTTCGCTGGC CCACTGAGCT AGCAGTCTCC 
CCGATGGACA ACACGCTGCA CATAATCGAC GACCACATGA TCCTGCGGAT GACACCAGAC 
GGTCGTGTGC GCGTCATCTC CGGCCGTCCC CTACATTGCG CCACAGCCTC CACTGCCTAC 
GATACGGATC TGGCTACCCA CGCTACCCTG GTGATGCCGC AGTCTATCGC GTTCGGTCCA 
CTCGGTGAGC TTTACGTGGC GGAAAGCGAC TCTCAACGAA TAAACCGGGT GCGCGTGATC 
GGCACGGACG GAAGGATCGC TCCGTTTGCT GGTGCCGAAT CCAAGTGCAA TTGCCTGGAG 
CGCGGTTGCG ACTGCTTCGA AGCGGAACAC TATCTGGCCA CCAGTGCCAA GTTCAACACG 
ATCGCCGCCC TTGCTGTCAC ACCCGATAGC CATGTACACA TCGCGGACCA GGCCAACTAC 
CGTATCCGCT CCGTTATGTC GAGCATCCCA GAGGCAAGTC CGTCGCGCGA GTACGAGATC 
TACGCACCTG ACATGCAGGA GATATACATT TTCAACCGAT TCGGACAGCA CGTGTCCACA 
CGCAACATCC TGACCGGAGA GACGACCTAT GTGTTTACCT ACAACGTAAA CACCTCCAAC 
GGAAAACTGA GCACCGTAAC AGACGCGGCT GGCAACAAAG TGTTCCTGCT TCGAGATTAC 
ACTTCTCAGG TAAATTCCAT CGAGAACACG AAGGGTCAAA AGTGCCGCCT GCGCATGACC 
AGGATGAAAA TGCTGCACGA GCTGAGCACT CCAGACAACT ACAATGTGAC CTACGAATAT 
CACGGCCCCA CCGGTCTGCT ACGAACCAAG CTGGACTCCA CCGGACGATC CTACGTTTAC 
AACTACGATG AGTTTGGTCG CCTCACTTCT GCAGTGACTC CCACCGGTCG TGTCATCGAG 
CTGAGCTTCG ACCTGAGCGT GAAGGGAGCC CAGGTAAAGG TTTCGGAGAA CGCCCAGAAG 
GAAATGTCAC TGCTGATCCA AGGTGCCACC GTTATTGTGC GCAACGGAGC CGCCGAGTCG 
CGTACCACCG TTGACATGGA CGGCTCCACC ACAAGCATCA CTCCATGGGG CCACAACCTG 
CAGATGGAAG TGGCACCTTA TACCATTCTG GCAGAACAAA GTCCACTGCT TGGCGAGAGC 
TACCCGGTCC CGGCTAAGCA ACGCACTGAG ATTGCTGGTG ACTTGGCCAA CAGGTTTGAG 
TGGCGCTACT TTGTGCGCCG GCAGCAGCCG CTGCAAGCGG GAAAACAGAG CAAGGGACCG 
CCACGTCCCG TAACCGAAGT TGGACGTAAG CTGCGTGTGA ACGGAGACAA CGTGCTGACT 
TTGGAGTACG ACCGCGAGAC CCAGTCGGTG GTCGTTATGG TGGACGACAA GCAGGAGCTG 
CTTAACGTGA CCTACGATCG CACTTCCCGC CCCATCAGCT TCCGACCGCA GTCCGGCGAC 
TACGCGGACG TGGACTTGGA GTACGATCGG TTCGGACGTC TGGTCAGTTG GAAGTGGGGT 
GTCCTGCAAG AAGCCTACTC TTTCGACCGC AATGGCCGTC TGAACGAGAT CAAGTACGGC 
GATGGCTCAA CAATGGTCTA CGCATTCAAG GACATGTTTG GCTCGTTACC GCTGAAGGTG 
ACTACGCCTA GACGTTCTGA TTATCTCCTG CAGTACGATG ATGCAGGTGC ACTCCAGAGC 
CTGACGACAC CTCGTGGACA CATTCACGCA TTCTCCCTGC AAACTTCTCT TGGCTTCTTT 
AAGTACCAAT ACTATTCACC AATCAACCGA CACCCCTTCG AGATTCTATA TAACGACGAA 
GGACAGATCT TGGCCAAAAT CCACCCCCAC CAATCTGGCA AGGTGGCATT CGTACATGAT 
ACTGCCGGAC GACTGGAGAC GATCCTCGCA GGATTGTCCA GCACCCACTA TACCTACCAG 
GATACAACTA GCCTGGTAAA GTCCGTGGAG GTGCAGGAGC CGGGCTTCGA GTTGCGCCGA 
GAGTTTAAAT ACCATGCAGG TATCCTAAAG GACGAGAAGT TGCGCTTCGG CTCCAAGAAC 
TCGCTGGCCT CGGCGCGCTA CAAGTACGCC TACGACGGAA ACGCCCGTCT CAGCGGCATC 
GAGATGGCCA TCGACGATAA GGAATTACCA ACCACGCGGT ACAAGTACAG CCAAAACTTG 
GGACAACTGG AGGTCGTGCA GGACCTGAAG ATCACGCGCA ACGCTTTTAA CCGAACAGTC 
ATACAGGACT CTGCCAAACA ATTCTTCGCC ATTGTGGACT ACGACCAGCA TGGACGGGTT 
AAGAGCGTGC TAATGAACGT GAAGAACATT GATGTGTTCC GCCTTGAGTT GGACTACGAT 
CTTCGCAACC GCATCAAGTC GCAAAAGACG ACCTTCGGTA GATCGACAGC CTTTGATAAG 
ATTAACTACA ATGCCGACGG ACATGTTGTT GAGGTCCTGG GCACCAACAA CTGGAAGTAT 
CTGTTCGACG AGAACGGCAA CACTGTTGGC GTGGTTGACC AGGGTGAGAA GTTCAACCTG 
GGCTACGACA TCGGAGACCG GGTGATCAAG GTAGGCGATG TGGAGTTCAA CAACTACGAT 
GCCCGCGGCT TCGTTGTGAA GCGCGGTGAG CAGAAGTACC GCTACAACAA CCGCGGACAA 
CTGATTCACT CGTTCGAAAG GGAACGTTTC CAGAGCTGGT ACTACTACGA CGACCGCAGC 
CGCCTCGTGG CCTGGCATGA TAACAAAGGT AACACCACGC AGTACTACTA CGCCAATCCT 
CGCACTCCTC ATCTCGTGAC CCACGTTCAC TTCCCGAAGA TCAGCCGCAC CATGAAGCTG 
TTCTACGATG ACCGCGACAT GCTAATCGCT TTGGAGCATG AGGACCAGCG CTACTACGTG 
GCCACCGACC AGAACGGCTC GCCACTAGCC TTCTTTGATC AGAATGGCAG CATTGTCAAG 
GAAATGAAGC GAACGCCCTT CGGCCGAATC ATTAAGGACA CTAAGCCGGA GTTCTTTGTG 
CCCATCGACT TCCACGGCGG ACTGATCGAC CCACACACAA AGCTGGTGTA CACCGAGCAG 
CGGCAGTACG ACCCGCACGT TGGCCAATGG ATGACCCCGT TGTGGGAGAC GCTCGCCACC 
GAGATGTCAC ACCCGACGGA TGTTTTTATA TACCGCTACC ACAACAACGA CCCCATCAAC 
CCGAACAAGC CCCAAAACTA TATGATCGAT CTGGATTCCT GGCTCCAGTT ATTCGGCTAC 
GATTTGAACA ACATGCAAAG CAGCCGCTAC ACCAAGCTGG CCCAGTATAC GCCGCAGGCT 
TCCATCAAGT CAAACACGTT GGCCCCCGAC TTTGGCGTGA TCTCCGGCCT GGAGTGCATC 
GTGGAAAAAA CAAGTGAGAA GTTCAGTGAT TTTGACTTTG TGCCCAAACC GCTGCTGAAA 
ATGGAGCCAA AGATGCGCAA CTTGCTCCCG CGTGTCAGCT ACCGGCGCGG TGTGTTCGGC 
GAAGGTGTAC TCCTCTCGAG AATCGGCGGA CGGGCGTTAG TTAGCGTGGT CGACGGATCA 
AACAGCGTGG TGCAGGACGT AGTGAGCAGC GTGTTCAACA ACTCTTACTT CCTTGACCTA 
CACTTCAGCA TTCACGACCA AGACGTATTT TACTTCGTAA AGGATAATGT CCTGAAGCTG 
CGCGACGACA ACGAGGAGCT GCGTCGCCTT GGCGGCATGT TCAACATATC AACCCATGAA 
ATCAGCGACC ACGGAGGCAG CGCCGCTAAG GAGCTGCGTC TTCACGGCCC AGACGCCGTT 
GTCATTATCA AATATGGCGT TGATCCCGAG CAGGAGCGCC ACCGCATCCT AAAGCATGCC 
CACAAGCGGG CAGTGGAGCG GGCTTGGGAG TTGGAGAAGC AGCTGGTGGC TGCCGGCTTC 
CAAGGCCGTG GCGACTGGAC CGAGGAGGAG AAAGAGGAGC TCGTCCAACA CGGTGACGTC 
GACGGCTGGA ACGGAATCGA TATCCACAGC ATACACAAGT ATCCGCAGCT GGCCGACGAC 
CCCGGTAACG TGGCTTTCCA GCGGGACGCG AAGCGTAAGC GACGCAAGAC CGGCAGCAGC 
CATCGGAGTG CCTCCAACCG CCGCCAGCTC AAGTTCGGCG AGCTGAGTGC GTGAGTGGAG 
GCATATAGAG AGAAGCTGGT GGCGGAGACG AAGCCAGAGG AGAGTGACAT GACCGACGAA 
GAATACGGTG ACCAGGAGGA CTACCTAATT GCCGTGGGCA AAATGGAGCC AAGGGACAGC 
AGTGAGGCAC TTGACGAGCA GATTTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Ten-m cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報