Nopp140 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nopp140
Entrez Gene ID	40433
Full Name	CG7421 gene product from transcript CG7421-RE
Synonyms	CG7421,DmNopp140,Dmel\CG7421,Nopp- 140,nopp140
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 78F4-78F4

mRNA and Protein(s)

mRNA	Protein	Name
NM_168940.3	NP_730693.1	Nopp140, isoform B
NM_001275266.1	NP_001262195.1	Nopp140, isoform E
NM_168941.4	NP_730694.2	Nopp140, isoform A
NM_001202229.2	NP_001189158.2	Nopp140, isoform F

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nopp140 is not required for overall nucleolar integrity, but is required for 2'-O-methylation. Loss of Nopp140 impairs 2'-O-methylation of 18S and 28S rRNA.
Title: Deletion of Drosophila Nopp140 induces subcellular ribosomopathies.

occurrence of apoptosis, autophagy and phenoloxidase A3 release to the hemolymph upon nucleolar stress correlated well with the demonstrated activation of Jun N-terminal kinase (JNK) in Nopp140-depleted larvae
Title: Nucleolar stress in Drosophila melanogaster: RNAi-mediated depletion of Nopp140.

Data show that RNAi knockdown of Nopp140 induces Minute-like phenotypes in Drosophila that are reminiscent of human craniofacial malformations associated with Treacher Collins syndrome.
Title: RNAi knockdown of Nopp140 induces Minute-like phenotypes in Drosophila.

Pole cells contained GFP-Nopp140 during the syncytial embryonic stages, but their nucleologenesis started at gastrulation.
Title: Tracking nucleolar dynamics with GFP-Nopp140 during Drosophila oogenesis and embryogenesis.

Two splice variants of Nopp140
Title: Two splice variants of Nopp140 in Drosophila melanogaster.

The following Nopp140 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nopp140 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19237	NM_001275266.1 Latest version!	Drosophila melanogaster Nopp140, transcript variant E (Nopp140), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa19238	NM_001202229.2 Latest version!	Drosophila melanogaster Nopp140, transcript variant F (Nopp140), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa21222	NM_168940.3 Latest version!	Drosophila melanogaster Nopp140, transcript variant B (Nopp140), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa21224	NM_168941.4 Latest version!	Drosophila melanogaster Nopp140, transcript variant A (Nopp140), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nopp140 ( NM_001275266.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262195.1 Drosophila melanogaster Nopp140, transcript variant E (Nopp140), mRNA.

Price & Availability↑

CloneID	OFa19237
Clone ID Related Accession (Same CDS sequence)	NM_001275266.1
Accession Version	NM_001275266.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Nopp140, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGACC TGCTAAAGAT AGCCGATGCA ATCGTCCTGG AGTACTTGCA GTCCAAGGAC 
AAGAACCTAG CCAAGGTTTT CCAGCAGAAG ACGAAGGCGG CCAGTGTCGC CAAAAGCAGC 
CCAAAGTTGA GTGAAATCCT TCAGTTCTAC CAGACCAAAA GCCCCAAAAA GATCCCAGCA 
ATCAAGGCGA CAGCCGGAGA CTCCAGCGAA GACAGCGACT CAGACTCTGA GTCTGATGCG 
GCGCCTAAGA AGCCAGCGAC AGCTCCTGCA CTAACAAACG GCAAGGCTGT CAAAAAGGCA 
GCTTCTTCAA CCAGCGAGGA CAGCGATTCA GAGGAGGAGA AGAAGCCAGC AGCAAAGGCC 
ACGCCGGCCA AGGCTGTCGG CAAGAAGGCA AAATCCTCCA GCGAGGACAG TTCCTCGGAG 
GAAGAAGCAC CCAAAAAAGC TGCTCCAGTG AAGGCACCTC CGGCCAAGGC GGCTCCCGCG 
AAGAAGGTTG AGTCCAGCAG CGAGGATAGC AGTTCCGAGG AAGAACCGGC AAAGCCCGCC 
GTCAAGGCCA CAACGACTAA GGTTGCTCCC GCCAAGAAGG CCGACTCCAG CAGCGAAGAA 
AGCAGCTCCG ATGAGGAGAC TAAGCCAGCT GCCAAGCCTG TAGCCAAGGC TGCACCGGCA 
AAGAAAGCAG CATCCTCCAG CGAGGAAAGC GACTCCGATG ACGAGCCAGC CGCCAAGAAG 
CCTGCCGTCC AGCCTGCCGC AAAGCCAGCT CCTAAGGCAG CCGCTTCTAG TTCCGAAGAC 
AGCAGCTCAG AGGAGGAGGT CAAACCCGCT GCTAAATCTG CTGCCAAGTT GGCTCCTGCT 
AAGAAGGGGG CTTCCTCTAG TGATGATAGT TCCTCTGAAG ACGAGGCACC CAAAAAGGCA 
GCAACTCTCG CAAAGCCCAT TTCTAAGGCT GCTCCCACCA AAAAGGCCGA CAGTTCCACT 
GAGGATAGTT CTTCGGAAGA CGATGCTCCT AAGAAGGTAG CTCCAGCAAA GGCTACGCCA 
GCTAAGGCAA TTCCTGCCAA GAAGGCAGCT TCCAGCGATG ACAGCTCCTC GGAGGAAGAG 
GCGCCCAAGA AGGCAGCTCC AGCAAATGCC ACGCCAGCTA GGGCACCTCC TGCCAAGAAG 
GCAGCTTCCA GCGATGACAG CTCCTCGGAA GAAGAGGCGC CCAAGAAGGC AGCTCCAGCA 
AAGGCCACGC CAGCTAAGGC AACTCCTGCC AAGAAGGCAG CTTCCAGCGA TGACAGCTCC 
TCGGAAGAAG AGGCGCCCAA GAAGGCTGCC GCCCCAGCAA AGGCGACACC GGCCAAAAAG 
GCCAAGTCTT CAAGCGAAGA CAGCGACTCC GATGAGGAGG AAGCTCCCAA GAAACCAGCC 
GCGAAGGCCG TTGCAAAGGC TGCTTCTTCG GAAGATAGCG ATAGCTCAGA AGACGAAAAG 
CCAGCAAAGG CTGCTCCCAA GGCTCTGGCC AAGTCTGCAA AGGCTGCCTC CTCAGACAGT 
GACGATTCCA GCGATGAAGA AACGCCGGCA GTGAAGCCAG CTGTCAAGAA GACTGCTGCT 
CCTGCGAAGA AGGCTGACAG CAGCAGCGAC GAGAGTGACT CTGGTGAAGA GTCCGGCGAG 
GTCAAGCCCA ACTCAGCTAC CAATGGCAAT GAGAAGACCG CTCAGAAGCG CAAGTTTAGT 
GGTGGCGACC AGGACGAGGC CACTCCCAAC AAGAAGTACA ACAACTTCGT CAAATCGGGA 
GAGCAACAGA AGAATGACTT CACCTCCACA CCTAACAACA CCTTCAGCCG AAACCATAAT 
ATGAACAATA GCGGAGGGGG AAGTGGGCGA CGGTCGCCCT TCCGAAGGGT ACGCACCGAG 
GACGTGGTGG TGGACTCCCG AGTCCAGGAC ATGTCCTTCG AGGCGAAGGA AAACGACTTT 
AAGAAGCACA ACAACGGACG GGGCGGCCGA GGAGGCTCAG GCTTCTCCGG ACGACCGGAT 
CGCAGCACTT GGGAGACCAA CAAGTTCAAC GGCGAGGGCG GCGGTGATGG AGGCGGCTTC 
AAGAAGATTG GCGATCGCAA GAGCTTTGGA GGCTTTGATA ACAACCAGCG CGGAGGACGT 
GGTGGTGGCG GTCGTGGAGG CGGCGGAGGC TTCGGTGGTC GTGGCGGTGG CGGCCGTGGC 
GGCGGTGGTG GCTTCGGAGG ACGTGGTGGC GGCGGACGCG GAGGCGGCGG CTTTGGAGGA 
CGCGGGGGCC GCGGTGGAGG CGGACGTGGA GGCGGATTCG GCAACAAATC CTTCGACTCG 
TCGGCGCCAA AGCAAAACAA GAAGATCACC TTCGACAATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262195.1
CDS	106..2427
Translation	MTDLLKIADAIVLEYLQSKDKNLAKVFQQKTKAASVAKSSPKLSEILQFYQTKSPKKIPAIKATAGDSSEDSDSDSESDAAPKKPATAPALTNGKAVKKAASSTSEDSDSEEEKKPAAKATPAKAVGKKAKSSSEDSSSEEEAPKKAAPVKAPPAKAAPAKKVESSSEDSSSEEEPAKPAVKATTTKVAPAKKADSSSEESSSDEETKPAAKPVAKAAPAKKAASSSEESDSDDEPAAKKPAVQPAAKPAPKAAASSSEDSSSEEEVKPAAKSAAKLAPAKKGASSSDDSSSEDEAPKKAATLAKPISKAAPTKKADSSTEDSSSEDDAPKKVAPAKATPAKAIPAKKAASSDDSSSEEEAPKKAAPANATPARAPPAKKAASSDDSSSEEEAPKKAAPAKATPAKATPAKKAASSDDSSSEEEAPKKAAAPAKATPAKKAKSSSEDSDSDEEEAPKKPAAKAVAKAASSEDSDSSEDEKPAKAAPKALAKSAKAASSDSDDSSDEETPAVKPAVKKTAAPAKKADSSSDESDSGEESGEVKPNSATNGNEKTAQKRKFSGGDQDEATPNKKYNNFVKSGEQQKNDFTSTPNNTFSRNHNMNNSGGGSGRRSPFRRVRTEDVVVDSRVQDMSFEAKENDFKKHNNGRGGRGGSGFSGRPDRSTWETNKFNGEGGGDGGGFKKIGDRKSFGGFDNNQRGGRGGGGRGGGGGFGGRGGGGRGGGGGFGGRGGGGRGGGGFGGRGGRGGGGRGGGFGNKSFDSSAPKQNKKITFDN

Target ORF information:

RefSeq Version	NM_001275266.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Nopp140, transcript variant E (Nopp140), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275266.1

ORF Insert Sequence:

ATGACAGACC TGCTAAAGAT AGCCGATGCA ATCGTCCTGG AGTACTTGCA GTCCAAGGAC 
AAGAACCTAG CCAAGGTTTT CCAGCAGAAG ACGAAGGCGG CCAGTGTCGC CAAAAGCAGC 
CCAAAGTTGA GTGAAATCCT TCAGTTCTAC CAGACCAAAA GCCCCAAAAA GATCCCAGCA 
ATCAAGGCGA CAGCCGGAGA CTCCAGCGAA GACAGCGACT CAGACTCTGA GTCTGATGCG 
GCGCCTAAGA AGCCAGCGAC AGCTCCTGCA CTAACAAACG GCAAGGCTGT CAAAAAGGCA 
GCTTCTTCAA CCAGCGAGGA CAGCGATTCA GAGGAGGAGA AGAAGCCAGC AGCAAAGGCC 
ACGCCGGCCA AGGCTGTCGG CAAGAAGGCA AAATCCTCCA GCGAGGACAG TTCCTCGGAG 
GAAGAAGCAC CCAAAAAAGC TGCTCCAGTG AAGGCACCTC CGGCCAAGGC GGCTCCCGCG 
AAGAAGGTTG AGTCCAGCAG CGAGGATAGC AGTTCCGAGG AAGAACCGGC AAAGCCCGCC 
GTCAAGGCCA CAACGACTAA GGTTGCTCCC GCCAAGAAGG CCGACTCCAG CAGCGAAGAA 
AGCAGCTCCG ATGAGGAGAC TAAGCCAGCT GCCAAGCCTG TAGCCAAGGC TGCACCGGCA 
AAGAAAGCAG CATCCTCCAG CGAGGAAAGC GACTCCGATG ACGAGCCAGC CGCCAAGAAG 
CCTGCCGTCC AGCCTGCCGC AAAGCCAGCT CCTAAGGCAG CCGCTTCTAG TTCCGAAGAC 
AGCAGCTCAG AGGAGGAGGT CAAACCCGCT GCTAAATCTG CTGCCAAGTT GGCTCCTGCT 
AAGAAGGGGG CTTCCTCTAG TGATGATAGT TCCTCTGAAG ACGAGGCACC CAAAAAGGCA 
GCAACTCTCG CAAAGCCCAT TTCTAAGGCT GCTCCCACCA AAAAGGCCGA CAGTTCCACT 
GAGGATAGTT CTTCGGAAGA CGATGCTCCT AAGAAGGTAG CTCCAGCAAA GGCTACGCCA 
GCTAAGGCAA TTCCTGCCAA GAAGGCAGCT TCCAGCGATG ACAGCTCCTC GGAGGAAGAG 
GCGCCCAAGA AGGCAGCTCC AGCAAATGCC ACGCCAGCTA GGGCACCTCC TGCCAAGAAG 
GCAGCTTCCA GCGATGACAG CTCCTCGGAA GAAGAGGCGC CCAAGAAGGC AGCTCCAGCA 
AAGGCCACGC CAGCTAAGGC AACTCCTGCC AAGAAGGCAG CTTCCAGCGA TGACAGCTCC 
TCGGAAGAAG AGGCGCCCAA GAAGGCTGCC GCCCCAGCAA AGGCGACACC GGCCAAAAAG 
GCCAAGTCTT CAAGCGAAGA CAGCGACTCC GATGAGGAGG AAGCTCCCAA GAAACCAGCC 
GCGAAGGCCG TTGCAAAGGC TGCTTCTTCG GAAGATAGCG ATAGCTCAGA AGACGAAAAG 
CCAGCAAAGG CTGCTCCCAA GGCTCTGGCC AAGTCTGCAA AGGCTGCCTC CTCAGACAGT 
GACGATTCCA GCGATGAAGA AACGCCGGCA GTGAAGCCAG CTGTCAAGAA GACTGCTGCT 
CCTGCGAAGA AGGCTGACAG CAGCAGCGAC GAGAGTGACT CTGGTGAAGA GTCCGGCGAG 
GTCAAGCCCA ACTCAGCTAC CAATGGCAAT GAGAAGACCG CTCAGAAGCG CAAGTTTAGT 
GGTGGCGACC AGGACGAGGC CACTCCCAAC AAGAAGTACA ACAACTTCGT CAAATCGGGA 
GAGCAACAGA AGAATGACTT CACCTCCACA CCTAACAACA CCTTCAGCCG AAACCATAAT 
ATGAACAATA GCGGAGGGGG AAGTGGGCGA CGGTCGCCCT TCCGAAGGGT ACGCACCGAG 
GACGTGGTGG TGGACTCCCG AGTCCAGGAC ATGTCCTTCG AGGCGAAGGA AAACGACTTT 
AAGAAGCACA ACAACGGACG GGGCGGCCGA GGAGGCTCAG GCTTCTCCGG ACGACCGGAT 
CGCAGCACTT GGGAGACCAA CAAGTTCAAC GGCGAGGGCG GCGGTGATGG AGGCGGCTTC 
AAGAAGATTG GCGATCGCAA GAGCTTTGGA GGCTTTGATA ACAACCAGCG CGGAGGACGT 
GGTGGTGGCG GTCGTGGAGG CGGCGGAGGC TTCGGTGGTC GTGGCGGTGG CGGCCGTGGC 
GGCGGTGGTG GCTTCGGAGG ACGTGGTGGC GGCGGACGCG GAGGCGGCGG CTTTGGAGGA 
CGCGGGGGCC GCGGTGGAGG CGGACGTGGA GGCGGATTCG GCAACAAATC CTTCGACTCG 
TCGGCGCCAA AGCAAAACAA GAAGATCACC TTCGACAATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nopp140 ( NM_001202229.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189158.2 Drosophila melanogaster Nopp140, transcript variant F (Nopp140), mRNA.

Price & Availability↑

CloneID	OFa19238
Clone ID Related Accession (Same CDS sequence)	NM_001202229.2
Accession Version	NM_001202229.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Nopp140, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001202229.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGACC TGCTAAAGAT AGCCGATGCA ATCGTCCTGG AGTACTTGCA GTCCAAGGAC 
AAGAACCTAG CCAAGGTTTT CCAGCAGAAG ACGAAGGCGG CCAGTGTCGC CAAAAGCAGC 
CCAAAGTTGA GTGAAATCCT TCAGTTCTAC CAGACCAAAA GCCCCAAAAA GATCCCAGCA 
ATCAAGGCGA CAGCCGGAGA CTCCAGCGAA GACAGCGACT CAGACTCTGA GTCTGATGCG 
GCGCCTAAGA AGCCAGCGAC AGCTCCTGCA CTAACAAACG GCAAGGCTGT CAAAAAGGCA 
GCTTCTTCAA CCAGCGAGGA CAGCGATTCA GAGGAGGAGA AGAAGCCAGC AGCAAAGGCC 
ACGCCGGCCA AGGCTGTCGG CAAGAAGGCA AAATCCTCCA GCGAGGACAG TTCCTCGGAG 
GAAGAAGCAC CCAAAAAAGC TGCTCCAGTG AAGGCACCTC CGGCCAAGGC GGCTCCCGCG 
AAGAAGGTTG AGTCCAGCAG CGAGGATAGC AGTTCCGAGG AAGAACCGGC AAAGCCCGCC 
GTCAAGGCCA CAACGACTAA GGTTGCTCCC GCCAAGAAGG CCGACTCCAG CAGCGAAGAA 
AGCAGCTCCG ATGAGGAGAC TAAGCCAGCT GCCAAGCCTG TAGCCAAGGC TGCACCGGCA 
AAGAAAGCAG CATCCTCCAG CGAGGAAAGC GACTCCGATG ACGAGCCAGC CGCCAAGAAG 
CCTGCCGTCC AGCCTGCCGC AAAGCCAGCT CCTAAGGCAG CCGCTTCTAG TTCCGAAGAC 
AGCAGCTCAG AGGAGGAGGT CAAACCCGCT GCTAAATCTG CTGCCAAGTT GGCTCCTGCT 
AAGAAGGGGG CTTCCTCTAG TGATGATAGT TCCTCTGAAG ACGAGGCACC CAAAAAGGCA 
GCAACTCTCG CAAAGCCCAT TTCTAAGGCT GCTCCCACCA AAAAGGCCGA CAGTTCCACT 
GAGGATAGTT CTTCGGAAGA CGATGCTCCT AAGAAGGTAG CTCCAGCAAA GGCTACGCCA 
GCTAAGGCAA TTCCTGCCAA GAAGGCAGCT TCCAGCGATG ACAGCTCCTC GGAGGAAGAG 
GCGCCCAAGA AGGCAGCTCC AGCAAATGCC ACGCCAGCTA GGGCACCTCC TGCCAAGAAG 
GCAGCTTCCA GCGATGACAG CTCCTCGGAA GAAGAGGCGC CCAAGAAGGC AGCTCCAGCA 
AAGGCCACGC CAGCTAAGGC AACTCCTGCC AAGAAGGCAG CTTCCAGCGA TGACAGCTCC 
TCGGAAGAAG AGGCGCCCAA GAAGGCTGCC GCCCCAGCAA AGGCGACACC GGCCAAAAAG 
GCCAAGTCTT CAAGCGAAGA CAGCGACTCC GATGAGGAGG AAGCTCCCAA GAAACCAGCC 
GCGAAGGCCG TTGCAAAGGC TGCTTCTTCG GAAGATAGCG ATAGCTCAGA AGACGAAAAG 
CCAGCAAAGG CTGCTCCCAA GGCTCTGGCC AAGTCTGCAA AGGCTGCCTC CTCAGACAGT 
GACGATTCCA GCGATGAAGA AACGCCGGCA GTGAAGCCAG CTGTCAAGAA GACTGCTGCT 
CCTGCGAAGA AGGCTGACAG CAGCAGCGAC GAGAGTGACT CTGGTGAAGA GTCCGGCGAG 
GTCAAGCCCA ACTCAGCTAC CAATGGCAAT GAGAAGACCG CTCAGAAGCG CAAGTTTAGT 
GGTGGCGACC AGGACGAGGC CACTCCCAAC AAGAAGTACA ACAACTTCGT CAAATCGGGA 
GAGCAACAGA ATGACTTCAC CTCCACACCT AACAACACCT TCAGCCGAAA CCATAATATG 
AACAATAGCG GAGGGGGAAG TGGGCGACGG TCGCCCTTCC GAAGGGTACG CACCGAGGAC 
GTGGTGGTGG ACTCCCGAGT CCAGGACATG TCCTTCGAGG CGAAGAAAAA CGCTGCCGGA 
TCCTGGGGCG AGCGAGCCAA CAAAGATCTG AAGCACACGC GCGGCAAGTC CTTCAAACAC 
GAGAAGACCA AGAAGAAGCG CGGCAGCTAC CGAGGTGGCC AAATTGACGT AGGAGTCAAT 
TCAATAAAAT TTGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189158.2
CDS	106..2163
Translation	MTDLLKIADAIVLEYLQSKDKNLAKVFQQKTKAASVAKSSPKLSEILQFYQTKSPKKIPAIKATAGDSSEDSDSDSESDAAPKKPATAPALTNGKAVKKAASSTSEDSDSEEEKKPAAKATPAKAVGKKAKSSSEDSSSEEEAPKKAAPVKAPPAKAAPAKKVESSSEDSSSEEEPAKPAVKATTTKVAPAKKADSSSEESSSDEETKPAAKPVAKAAPAKKAASSSEESDSDDEPAAKKPAVQPAAKPAPKAAASSSEDSSSEEEVKPAAKSAAKLAPAKKGASSSDDSSSEDEAPKKAATLAKPISKAAPTKKADSSTEDSSSEDDAPKKVAPAKATPAKAIPAKKAASSDDSSSEEEAPKKAAPANATPARAPPAKKAASSDDSSSEEEAPKKAAPAKATPAKATPAKKAASSDDSSSEEEAPKKAAAPAKATPAKKAKSSSEDSDSDEEEAPKKPAAKAVAKAASSEDSDSSEDEKPAKAAPKALAKSAKAASSDSDDSSDEETPAVKPAVKKTAAPAKKADSSSDESDSGEESGEVKPNSATNGNEKTAQKRKFSGGDQDEATPNKKYNNFVKSGEQQNDFTSTPNNTFSRNHNMNNSGGGSGRRSPFRRVRTEDVVVDSRVQDMSFEAKKNAAGSWGERANKDLKHTRGKSFKHEKTKKKRGSYRGGQIDVGVNSIKFD

Target ORF information:

RefSeq Version	NM_001202229.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Nopp140, transcript variant F (Nopp140), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202229.2

ORF Insert Sequence:

ATGACAGACC TGCTAAAGAT AGCCGATGCA ATCGTCCTGG AGTACTTGCA GTCCAAGGAC 
AAGAACCTAG CCAAGGTTTT CCAGCAGAAG ACGAAGGCGG CCAGTGTCGC CAAAAGCAGC 
CCAAAGTTGA GTGAAATCCT TCAGTTCTAC CAGACCAAAA GCCCCAAAAA GATCCCAGCA 
ATCAAGGCGA CAGCCGGAGA CTCCAGCGAA GACAGCGACT CAGACTCTGA GTCTGATGCG 
GCGCCTAAGA AGCCAGCGAC AGCTCCTGCA CTAACAAACG GCAAGGCTGT CAAAAAGGCA 
GCTTCTTCAA CCAGCGAGGA CAGCGATTCA GAGGAGGAGA AGAAGCCAGC AGCAAAGGCC 
ACGCCGGCCA AGGCTGTCGG CAAGAAGGCA AAATCCTCCA GCGAGGACAG TTCCTCGGAG 
GAAGAAGCAC CCAAAAAAGC TGCTCCAGTG AAGGCACCTC CGGCCAAGGC GGCTCCCGCG 
AAGAAGGTTG AGTCCAGCAG CGAGGATAGC AGTTCCGAGG AAGAACCGGC AAAGCCCGCC 
GTCAAGGCCA CAACGACTAA GGTTGCTCCC GCCAAGAAGG CCGACTCCAG CAGCGAAGAA 
AGCAGCTCCG ATGAGGAGAC TAAGCCAGCT GCCAAGCCTG TAGCCAAGGC TGCACCGGCA 
AAGAAAGCAG CATCCTCCAG CGAGGAAAGC GACTCCGATG ACGAGCCAGC CGCCAAGAAG 
CCTGCCGTCC AGCCTGCCGC AAAGCCAGCT CCTAAGGCAG CCGCTTCTAG TTCCGAAGAC 
AGCAGCTCAG AGGAGGAGGT CAAACCCGCT GCTAAATCTG CTGCCAAGTT GGCTCCTGCT 
AAGAAGGGGG CTTCCTCTAG TGATGATAGT TCCTCTGAAG ACGAGGCACC CAAAAAGGCA 
GCAACTCTCG CAAAGCCCAT TTCTAAGGCT GCTCCCACCA AAAAGGCCGA CAGTTCCACT 
GAGGATAGTT CTTCGGAAGA CGATGCTCCT AAGAAGGTAG CTCCAGCAAA GGCTACGCCA 
GCTAAGGCAA TTCCTGCCAA GAAGGCAGCT TCCAGCGATG ACAGCTCCTC GGAGGAAGAG 
GCGCCCAAGA AGGCAGCTCC AGCAAATGCC ACGCCAGCTA GGGCACCTCC TGCCAAGAAG 
GCAGCTTCCA GCGATGACAG CTCCTCGGAA GAAGAGGCGC CCAAGAAGGC AGCTCCAGCA 
AAGGCCACGC CAGCTAAGGC AACTCCTGCC AAGAAGGCAG CTTCCAGCGA TGACAGCTCC 
TCGGAAGAAG AGGCGCCCAA GAAGGCTGCC GCCCCAGCAA AGGCGACACC GGCCAAAAAG 
GCCAAGTCTT CAAGCGAAGA CAGCGACTCC GATGAGGAGG AAGCTCCCAA GAAACCAGCC 
GCGAAGGCCG TTGCAAAGGC TGCTTCTTCG GAAGATAGCG ATAGCTCAGA AGACGAAAAG 
CCAGCAAAGG CTGCTCCCAA GGCTCTGGCC AAGTCTGCAA AGGCTGCCTC CTCAGACAGT 
GACGATTCCA GCGATGAAGA AACGCCGGCA GTGAAGCCAG CTGTCAAGAA GACTGCTGCT 
CCTGCGAAGA AGGCTGACAG CAGCAGCGAC GAGAGTGACT CTGGTGAAGA GTCCGGCGAG 
GTCAAGCCCA ACTCAGCTAC CAATGGCAAT GAGAAGACCG CTCAGAAGCG CAAGTTTAGT 
GGTGGCGACC AGGACGAGGC CACTCCCAAC AAGAAGTACA ACAACTTCGT CAAATCGGGA 
GAGCAACAGA ATGACTTCAC CTCCACACCT AACAACACCT TCAGCCGAAA CCATAATATG 
AACAATAGCG GAGGGGGAAG TGGGCGACGG TCGCCCTTCC GAAGGGTACG CACCGAGGAC 
GTGGTGGTGG ACTCCCGAGT CCAGGACATG TCCTTCGAGG CGAAGAAAAA CGCTGCCGGA 
TCCTGGGGCG AGCGAGCCAA CAAAGATCTG AAGCACACGC GCGGCAAGTC CTTCAAACAC 
GAGAAGACCA AGAAGAAGCG CGGCAGCTAC CGAGGTGGCC AAATTGACGT AGGAGTCAAT 
TCAATAAAAT TTGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nopp140 ( NM_168940.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730693.1 Drosophila melanogaster Nopp140, transcript variant B (Nopp140), mRNA.

Price & Availability↑

CloneID	OFa21222
Clone ID Related Accession (Same CDS sequence)	NM_168940.3
Accession Version	NM_168940.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Nopp140, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168940.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGACC TGCTAAAGAT AGCCGATGCA ATCGTCCTGG AGTACTTGCA GTCCAAGGAC 
AAGAACCTAG CCAAGGTTTT CCAGCAGAAG ACGAAGGCGG CCAGTGTCGC CAAAAGCAGC 
CCAAAGTTGA GTGAAATCCT TCAGTTCTAC CAGACCAAAA GCCCCAAAAA GATCCCAGCA 
ATCAAGGCGA CAGCCGGAGA CTCCAGCGAA GACAGCGACT CAGACTCTGA GTCTGATGCG 
GCGCCTAAGA AGCCAGCGAC AGCTCCTGCA CTAACAAACG GCAAGGCTGT CAAAAAGGCA 
GCTTCTTCAA CCAGCGAGGA CAGCGATTCA GAGGAGGAGA AGAAGCCAGC AGCAAAGGCC 
ACGCCGGCCA AGGCTGTCGG CAAGAAGGCA AAATCCTCCA GCGAGGACAG TTCCTCGGAG 
GAAGAAGCAC CCAAAAAAGC TGCTCCAGTG AAGGCACCTC CGGCCAAGGC GGCTCCCGCG 
AAGAAGGTTG AGTCCAGCAG CGAGGATAGC AGTTCCGAGG AAGAACCGGC AAAGCCCGCC 
GTCAAGGCCA CAACGACTAA GGTTGCTCCC GCCAAGAAGG CCGACTCCAG CAGCGAAGAA 
AGCAGCTCCG ATGAGGAGAC TAAGCCAGCT GCCAAGCCTG TAGCCAAGGC TGCACCGGCA 
AAGAAAGCAG CATCCTCCAG CGAGGAAAGC GACTCCGATG ACGAGCCAGC CGCCAAGAAG 
CCTGCCGTCC AGCCTGCCGC AAAGCCAGCT CCTAAGGCAG CCGCTTCTAG TTCCGAAGAC 
AGCAGCTCAG AGGAGGAGGT CAAACCCGCT GCTAAATCTG CTGCCAAGTT GGCTCCTGCT 
AAGAAGGGGG CTTCCTCTAG TGATGATAGT TCCTCTGAAG ACGAGGCACC CAAAAAGGCA 
GCAACTCTCG CAAAGCCCAT TTCTAAGGCT GCTCCCACCA AAAAGGCCGA CAGTTCCACT 
GAGGATAGTT CTTCGGAAGA CGATGCTCCT AAGAAGGTAG CTCCAGCAAA GGCTACGCCA 
GCTAAGGCAA TTCCTGCCAA GAAGGCAGCT TCCAGCGATG ACAGCTCCTC GGAGGAAGAG 
GCGCCCAAGA AGGCAGCTCC AGCAAATGCC ACGCCAGCTA GGGCACCTCC TGCCAAGAAG 
GCAGCTTCCA GCGATGACAG CTCCTCGGAA GAAGAGGCGC CCAAGAAGGC AGCTCCAGCA 
AAGGCCACGC CAGCTAAGGC AACTCCTGCC AAGAAGGCAG CTTCCAGCGA TGACAGCTCC 
TCGGAAGAAG AGGCGCCCAA GAAGGCTGCC GCCCCAGCAA AGGCGACACC GGCCAAAAAG 
GCCAAGTCTT CAAGCGAAGA CAGCGACTCC GATGAGGAGG AAGCTCCCAA GAAACCAGCC 
GCGAAGGCCG TTGCAAAGGC TGCTTCTTCG GAAGATAGCG ATAGCTCAGA AGACGAAAAG 
CCAGCAAAGG CTGCTCCCAA GGCTCTGGCC AAGTCTGCAA AGGCTGCCTC CTCAGACAGT 
GACGATTCCA GCGATGAAGA AACGCCGGCA GTGAAGCCAG CTGTCAAGAA GACTGCTGCT 
CCTGCGAAGA AGGCTGACAG CAGCAGCGAC GAGAGTGACT CTGGTGAAGA GTCCGGCGAG 
GTCAAGCCCA ACTCAGCTAC CAATGGCAAT GAGAAGACCG CTCAGAAGCG CAAGTTTAGT 
GGTGGCGACC AGGACGAGGC CACTCCCAAC AAGAAGTACA ACAACTTCGT CAAATCGGGA 
GAGCAACAGA AGAATGACTT CACCTCCACA CCTAACAACA CCTTCAGCCG AAACCATAAT 
ATGAACAATA GCGGAGGGGG AAGTGGGCGA CGGTCGCCCT TCCGAAGGGT ACGCACCGAG 
GACGTGGTGG TGGACTCCCG AGTCCAGGAC ATGTCCTTCG AGGCGAAGAA AAACGCTGCC 
GGATCCTGGG GCGAGCGAGC CAACAAAGAT CTGAAGCACA CGCGCGGCAA GTCCTTCAAA 
CACGAGAAGA CCAAGAAGAA GCGCGGCAGC TACCGAGGTG GCCAAATTGA CGTAGGAGTC 
AATTCAATAA AATTTGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730693.1
CDS	106..2166
Translation	MTDLLKIADAIVLEYLQSKDKNLAKVFQQKTKAASVAKSSPKLSEILQFYQTKSPKKIPAIKATAGDSSEDSDSDSESDAAPKKPATAPALTNGKAVKKAASSTSEDSDSEEEKKPAAKATPAKAVGKKAKSSSEDSSSEEEAPKKAAPVKAPPAKAAPAKKVESSSEDSSSEEEPAKPAVKATTTKVAPAKKADSSSEESSSDEETKPAAKPVAKAAPAKKAASSSEESDSDDEPAAKKPAVQPAAKPAPKAAASSSEDSSSEEEVKPAAKSAAKLAPAKKGASSSDDSSSEDEAPKKAATLAKPISKAAPTKKADSSTEDSSSEDDAPKKVAPAKATPAKAIPAKKAASSDDSSSEEEAPKKAAPANATPARAPPAKKAASSDDSSSEEEAPKKAAPAKATPAKATPAKKAASSDDSSSEEEAPKKAAAPAKATPAKKAKSSSEDSDSDEEEAPKKPAAKAVAKAASSEDSDSSEDEKPAKAAPKALAKSAKAASSDSDDSSDEETPAVKPAVKKTAAPAKKADSSSDESDSGEESGEVKPNSATNGNEKTAQKRKFSGGDQDEATPNKKYNNFVKSGEQQKNDFTSTPNNTFSRNHNMNNSGGGSGRRSPFRRVRTEDVVVDSRVQDMSFEAKKNAAGSWGERANKDLKHTRGKSFKHEKTKKKRGSYRGGQIDVGVNSIKFD

Target ORF information:

RefSeq Version	NM_168940.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Nopp140, transcript variant B (Nopp140), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168940.3

ORF Insert Sequence:

ATGACAGACC TGCTAAAGAT AGCCGATGCA ATCGTCCTGG AGTACTTGCA GTCCAAGGAC 
AAGAACCTAG CCAAGGTTTT CCAGCAGAAG ACGAAGGCGG CCAGTGTCGC CAAAAGCAGC 
CCAAAGTTGA GTGAAATCCT TCAGTTCTAC CAGACCAAAA GCCCCAAAAA GATCCCAGCA 
ATCAAGGCGA CAGCCGGAGA CTCCAGCGAA GACAGCGACT CAGACTCTGA GTCTGATGCG 
GCGCCTAAGA AGCCAGCGAC AGCTCCTGCA CTAACAAACG GCAAGGCTGT CAAAAAGGCA 
GCTTCTTCAA CCAGCGAGGA CAGCGATTCA GAGGAGGAGA AGAAGCCAGC AGCAAAGGCC 
ACGCCGGCCA AGGCTGTCGG CAAGAAGGCA AAATCCTCCA GCGAGGACAG TTCCTCGGAG 
GAAGAAGCAC CCAAAAAAGC TGCTCCAGTG AAGGCACCTC CGGCCAAGGC GGCTCCCGCG 
AAGAAGGTTG AGTCCAGCAG CGAGGATAGC AGTTCCGAGG AAGAACCGGC AAAGCCCGCC 
GTCAAGGCCA CAACGACTAA GGTTGCTCCC GCCAAGAAGG CCGACTCCAG CAGCGAAGAA 
AGCAGCTCCG ATGAGGAGAC TAAGCCAGCT GCCAAGCCTG TAGCCAAGGC TGCACCGGCA 
AAGAAAGCAG CATCCTCCAG CGAGGAAAGC GACTCCGATG ACGAGCCAGC CGCCAAGAAG 
CCTGCCGTCC AGCCTGCCGC AAAGCCAGCT CCTAAGGCAG CCGCTTCTAG TTCCGAAGAC 
AGCAGCTCAG AGGAGGAGGT CAAACCCGCT GCTAAATCTG CTGCCAAGTT GGCTCCTGCT 
AAGAAGGGGG CTTCCTCTAG TGATGATAGT TCCTCTGAAG ACGAGGCACC CAAAAAGGCA 
GCAACTCTCG CAAAGCCCAT TTCTAAGGCT GCTCCCACCA AAAAGGCCGA CAGTTCCACT 
GAGGATAGTT CTTCGGAAGA CGATGCTCCT AAGAAGGTAG CTCCAGCAAA GGCTACGCCA 
GCTAAGGCAA TTCCTGCCAA GAAGGCAGCT TCCAGCGATG ACAGCTCCTC GGAGGAAGAG 
GCGCCCAAGA AGGCAGCTCC AGCAAATGCC ACGCCAGCTA GGGCACCTCC TGCCAAGAAG 
GCAGCTTCCA GCGATGACAG CTCCTCGGAA GAAGAGGCGC CCAAGAAGGC AGCTCCAGCA 
AAGGCCACGC CAGCTAAGGC AACTCCTGCC AAGAAGGCAG CTTCCAGCGA TGACAGCTCC 
TCGGAAGAAG AGGCGCCCAA GAAGGCTGCC GCCCCAGCAA AGGCGACACC GGCCAAAAAG 
GCCAAGTCTT CAAGCGAAGA CAGCGACTCC GATGAGGAGG AAGCTCCCAA GAAACCAGCC 
GCGAAGGCCG TTGCAAAGGC TGCTTCTTCG GAAGATAGCG ATAGCTCAGA AGACGAAAAG 
CCAGCAAAGG CTGCTCCCAA GGCTCTGGCC AAGTCTGCAA AGGCTGCCTC CTCAGACAGT 
GACGATTCCA GCGATGAAGA AACGCCGGCA GTGAAGCCAG CTGTCAAGAA GACTGCTGCT 
CCTGCGAAGA AGGCTGACAG CAGCAGCGAC GAGAGTGACT CTGGTGAAGA GTCCGGCGAG 
GTCAAGCCCA ACTCAGCTAC CAATGGCAAT GAGAAGACCG CTCAGAAGCG CAAGTTTAGT 
GGTGGCGACC AGGACGAGGC CACTCCCAAC AAGAAGTACA ACAACTTCGT CAAATCGGGA 
GAGCAACAGA AGAATGACTT CACCTCCACA CCTAACAACA CCTTCAGCCG AAACCATAAT 
ATGAACAATA GCGGAGGGGG AAGTGGGCGA CGGTCGCCCT TCCGAAGGGT ACGCACCGAG 
GACGTGGTGG TGGACTCCCG AGTCCAGGAC ATGTCCTTCG AGGCGAAGAA AAACGCTGCC 
GGATCCTGGG GCGAGCGAGC CAACAAAGAT CTGAAGCACA CGCGCGGCAA GTCCTTCAAA 
CACGAGAAGA CCAAGAAGAA GCGCGGCAGC TACCGAGGTG GCCAAATTGA CGTAGGAGTC 
AATTCAATAA AATTTGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nopp140 ( NM_168941.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_730694.2 Drosophila melanogaster Nopp140, transcript variant A (Nopp140), mRNA.

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CloneID	OFa21224
Clone ID Related Accession (Same CDS sequence)	NM_168941.4
Accession Version	NM_168941.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Nopp140, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168941.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACAGACC TGCTAAAGAT AGCCGATGCA ATCGTCCTGG AGTACTTGCA GTCCAAGGAC 
AAGAACCTAG CCAAGGTTTT CCAGCAGAAG ACGAAGGCGG CCAGTGTCGC CAAAAGCAGC 
CCAAAGTTGA GTGAAATCCT TCAGTTCTAC CAGACCAAAA GCCCCAAAAA GATCCCAGCA 
ATCAAGGCGA CAGCCGGAGA CTCCAGCGAA GACAGCGACT CAGACTCTGA GTCTGATGCG 
GCGCCTAAGA AGCCAGCGAC AGCTCCTGCA CTAACAAACG GCAAGGCTGT CAAAAAGGCA 
GCTTCTTCAA CCAGCGAGGA CAGCGATTCA GAGGAGGAGA AGAAGCCAGC AGCAAAGGCC 
ACGCCGGCCA AGGCTGTCGG CAAGAAGGCA AAATCCTCCA GCGAGGACAG TTCCTCGGAG 
GAAGAAGCAC CCAAAAAAGC TGCTCCAGTG AAGGCACCTC CGGCCAAGGC GGCTCCCGCG 
AAGAAGGTTG AGTCCAGCAG CGAGGATAGC AGTTCCGAGG AAGAACCGGC AAAGCCCGCC 
GTCAAGGCCA CAACGACTAA GGTTGCTCCC GCCAAGAAGG CCGACTCCAG CAGCGAAGAA 
AGCAGCTCCG ATGAGGAGAC TAAGCCAGCT GCCAAGCCTG TAGCCAAGGC TGCACCGGCA 
AAGAAAGCAG CATCCTCCAG CGAGGAAAGC GACTCCGATG ACGAGCCAGC CGCCAAGAAG 
CCTGCCGTCC AGCCTGCCGC AAAGCCAGCT CCTAAGGCAG CCGCTTCTAG TTCCGAAGAC 
AGCAGCTCAG AGGAGGAGGT CAAACCCGCT GCTAAATCTG CTGCCAAGTT GGCTCCTGCT 
AAGAAGGGGG CTTCCTCTAG TGATGATAGT TCCTCTGAAG ACGAGGCACC CAAAAAGGCA 
GCAACTCTCG CAAAGCCCAT TTCTAAGGCT GCTCCCACCA AAAAGGCCGA CAGTTCCACT 
GAGGATAGTT CTTCGGAAGA CGATGCTCCT AAGAAGGTAG CTCCAGCAAA GGCTACGCCA 
GCTAAGGCAA TTCCTGCCAA GAAGGCAGCT TCCAGCGATG ACAGCTCCTC GGAGGAAGAG 
GCGCCCAAGA AGGCAGCTCC AGCAAATGCC ACGCCAGCTA GGGCACCTCC TGCCAAGAAG 
GCAGCTTCCA GCGATGACAG CTCCTCGGAA GAAGAGGCGC CCAAGAAGGC AGCTCCAGCA 
AAGGCCACGC CAGCTAAGGC AACTCCTGCC AAGAAGGCAG CTTCCAGCGA TGACAGCTCC 
TCGGAAGAAG AGGCGCCCAA GAAGGCTGCC GCCCCAGCAA AGGCGACACC GGCCAAAAAG 
GCCAAGTCTT CAAGCGAAGA CAGCGACTCC GATGAGGAGG AAGCTCCCAA GAAACCAGCC 
GCGAAGGCCG TTGCAAAGGC TGCTTCTTCG GAAGATAGCG ATAGCTCAGA AGACGAAAAG 
CCAGCAAAGG CTGCTCCCAA GGCTCTGGCC AAGTCTGCAA AGGCTGCCTC CTCAGACAGT 
GACGATTCCA GCGATGAAGA AACGCCGGCA GTGAAGCCAG CTGTCAAGAA GACTGCTGCT 
CCTGCGAAGA AGGCTGACAG CAGCAGCGAC GAGAGTGACT CTGGTGAAGA GTCCGGCGAG 
GTCAAGCCCA ACTCAGCTAC CAATGGCAAT GAGAAGACCG CTCAGAAGCG CAAGTTTAGT 
GGTGGCGACC AGGACGAGGC CACTCCCAAC AAGAAGTACA ACAACTTCGT CAAATCGGGA 
GAGCAACAGG AAAACGACTT TAAGAAGCAC AACAACGGAC GGGGCGGCCG AGGAGGCTCA 
GGCTTCTCCG GACGACCGGA TCGCAGCACT TGGGAGACCA ACAAGTTCAA CGGCGAGGGC 
GGCGGTGATG GAGGCGGCTT CAAGAAGATT GGCGATCGCA AGAGCTTTGG AGGCTTTGAT 
AACAACCAGC GCGGAGGACG TGGTGGTGGC GGTCGTGGAG GCGGCGGAGG CTTCGGTGGT 
CGTGGCGGTG GCGGCCGTGG CGGCGGTGGT GGCTTCGGAG GACGTGGTGG CGGCGGACGC 
GGAGGCGGCG GCTTTGGAGG ACGCGGGGGC CGCGGTGGAG GCGGACGTGG AGGCGGATTC 
GGCAACAAAT CCTTCGACTC GTCGGCGCCA AAGCAAAACA AGAAGATCAC CTTCGACAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_730694.2
CDS	106..2268
Translation	MTDLLKIADAIVLEYLQSKDKNLAKVFQQKTKAASVAKSSPKLSEILQFYQTKSPKKIPAIKATAGDSSEDSDSDSESDAAPKKPATAPALTNGKAVKKAASSTSEDSDSEEEKKPAAKATPAKAVGKKAKSSSEDSSSEEEAPKKAAPVKAPPAKAAPAKKVESSSEDSSSEEEPAKPAVKATTTKVAPAKKADSSSEESSSDEETKPAAKPVAKAAPAKKAASSSEESDSDDEPAAKKPAVQPAAKPAPKAAASSSEDSSSEEEVKPAAKSAAKLAPAKKGASSSDDSSSEDEAPKKAATLAKPISKAAPTKKADSSTEDSSSEDDAPKKVAPAKATPAKAIPAKKAASSDDSSSEEEAPKKAAPANATPARAPPAKKAASSDDSSSEEEAPKKAAPAKATPAKATPAKKAASSDDSSSEEEAPKKAAAPAKATPAKKAKSSSEDSDSDEEEAPKKPAAKAVAKAASSEDSDSSEDEKPAKAAPKALAKSAKAASSDSDDSSDEETPAVKPAVKKTAAPAKKADSSSDESDSGEESGEVKPNSATNGNEKTAQKRKFSGGDQDEATPNKKYNNFVKSGEQQENDFKKHNNGRGGRGGSGFSGRPDRSTWETNKFNGEGGGDGGGFKKIGDRKSFGGFDNNQRGGRGGGGRGGGGGFGGRGGGGRGGGGGFGGRGGGGRGGGGFGGRGGRGGGGRGGGFGNKSFDSSAPKQNKKITFDN

Target ORF information:

RefSeq Version	NM_168941.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Nopp140, transcript variant A (Nopp140), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168941.4

ORF Insert Sequence:

ATGACAGACC TGCTAAAGAT AGCCGATGCA ATCGTCCTGG AGTACTTGCA GTCCAAGGAC 
AAGAACCTAG CCAAGGTTTT CCAGCAGAAG ACGAAGGCGG CCAGTGTCGC CAAAAGCAGC 
CCAAAGTTGA GTGAAATCCT TCAGTTCTAC CAGACCAAAA GCCCCAAAAA GATCCCAGCA 
ATCAAGGCGA CAGCCGGAGA CTCCAGCGAA GACAGCGACT CAGACTCTGA GTCTGATGCG 
GCGCCTAAGA AGCCAGCGAC AGCTCCTGCA CTAACAAACG GCAAGGCTGT CAAAAAGGCA 
GCTTCTTCAA CCAGCGAGGA CAGCGATTCA GAGGAGGAGA AGAAGCCAGC AGCAAAGGCC 
ACGCCGGCCA AGGCTGTCGG CAAGAAGGCA AAATCCTCCA GCGAGGACAG TTCCTCGGAG 
GAAGAAGCAC CCAAAAAAGC TGCTCCAGTG AAGGCACCTC CGGCCAAGGC GGCTCCCGCG 
AAGAAGGTTG AGTCCAGCAG CGAGGATAGC AGTTCCGAGG AAGAACCGGC AAAGCCCGCC 
GTCAAGGCCA CAACGACTAA GGTTGCTCCC GCCAAGAAGG CCGACTCCAG CAGCGAAGAA 
AGCAGCTCCG ATGAGGAGAC TAAGCCAGCT GCCAAGCCTG TAGCCAAGGC TGCACCGGCA 
AAGAAAGCAG CATCCTCCAG CGAGGAAAGC GACTCCGATG ACGAGCCAGC CGCCAAGAAG 
CCTGCCGTCC AGCCTGCCGC AAAGCCAGCT CCTAAGGCAG CCGCTTCTAG TTCCGAAGAC 
AGCAGCTCAG AGGAGGAGGT CAAACCCGCT GCTAAATCTG CTGCCAAGTT GGCTCCTGCT 
AAGAAGGGGG CTTCCTCTAG TGATGATAGT TCCTCTGAAG ACGAGGCACC CAAAAAGGCA 
GCAACTCTCG CAAAGCCCAT TTCTAAGGCT GCTCCCACCA AAAAGGCCGA CAGTTCCACT 
GAGGATAGTT CTTCGGAAGA CGATGCTCCT AAGAAGGTAG CTCCAGCAAA GGCTACGCCA 
GCTAAGGCAA TTCCTGCCAA GAAGGCAGCT TCCAGCGATG ACAGCTCCTC GGAGGAAGAG 
GCGCCCAAGA AGGCAGCTCC AGCAAATGCC ACGCCAGCTA GGGCACCTCC TGCCAAGAAG 
GCAGCTTCCA GCGATGACAG CTCCTCGGAA GAAGAGGCGC CCAAGAAGGC AGCTCCAGCA 
AAGGCCACGC CAGCTAAGGC AACTCCTGCC AAGAAGGCAG CTTCCAGCGA TGACAGCTCC 
TCGGAAGAAG AGGCGCCCAA GAAGGCTGCC GCCCCAGCAA AGGCGACACC GGCCAAAAAG 
GCCAAGTCTT CAAGCGAAGA CAGCGACTCC GATGAGGAGG AAGCTCCCAA GAAACCAGCC 
GCGAAGGCCG TTGCAAAGGC TGCTTCTTCG GAAGATAGCG ATAGCTCAGA AGACGAAAAG 
CCAGCAAAGG CTGCTCCCAA GGCTCTGGCC AAGTCTGCAA AGGCTGCCTC CTCAGACAGT 
GACGATTCCA GCGATGAAGA AACGCCGGCA GTGAAGCCAG CTGTCAAGAA GACTGCTGCT 
CCTGCGAAGA AGGCTGACAG CAGCAGCGAC GAGAGTGACT CTGGTGAAGA GTCCGGCGAG 
GTCAAGCCCA ACTCAGCTAC CAATGGCAAT GAGAAGACCG CTCAGAAGCG CAAGTTTAGT 
GGTGGCGACC AGGACGAGGC CACTCCCAAC AAGAAGTACA ACAACTTCGT CAAATCGGGA 
GAGCAACAGG AAAACGACTT TAAGAAGCAC AACAACGGAC GGGGCGGCCG AGGAGGCTCA 
GGCTTCTCCG GACGACCGGA TCGCAGCACT TGGGAGACCA ACAAGTTCAA CGGCGAGGGC 
GGCGGTGATG GAGGCGGCTT CAAGAAGATT GGCGATCGCA AGAGCTTTGG AGGCTTTGAT 
AACAACCAGC GCGGAGGACG TGGTGGTGGC GGTCGTGGAG GCGGCGGAGG CTTCGGTGGT 
CGTGGCGGTG GCGGCCGTGG CGGCGGTGGT GGCTTCGGAG GACGTGGTGG CGGCGGACGC 
GGAGGCGGCG GCTTTGGAGG ACGCGGGGGC CGCGGTGGAG GCGGACGTGG AGGCGGATTC 
GGCAACAAAT CCTTCGACTC GTCGGCGCCA AAGCAAAACA AGAAGATCAC CTTCGACAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Nopp140 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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