MED1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED1
Entrez Gene ID	40403
Full Name	Mediator complex subunit 1
Synonyms	CG7162,Dmel\CG7162,FBgn0037109,Med1,Med1/SOP3/TRAP220,Trap220,dTRAP220
General protein information	Preferred Names Mediator complex subunit 1 Names CG7162 gene product from transcript CG7162-RC CG7162-PA CG7162-PB CG7162-PC MED1-PA MED1-PB MED1-PC mediator mediator complex subunit 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 78E1-78E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_141084.3	NP_649341.1	mediator complex subunit 1, isoform A
NM_001275254.1	NP_001262183.1	mediator complex subunit 1, isoform C
NM_001202224.2	NP_001189153.1	mediator complex subunit 1, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MED1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19226	NM_001275254.1 Latest version!	Drosophila melanogaster mediator complex subunit 1, transcript variant C (MED1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa19227	NM_001202224.2 Latest version!	Drosophila melanogaster mediator complex subunit 1, transcript variant B (MED1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa21195	NM_141084.3 Latest version!	Drosophila melanogaster mediator complex subunit 1, transcript variant A (MED1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED1 ( NM_001275254.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262183.1 Drosophila melanogaster mediator complex subunit 1, transcript variant C (MED1), mRNA.

Price & Availability↑

CloneID	OFa19226
Clone ID Related Accession (Same CDS sequence)	NM_001275254.1
Accession Version	NM_001275254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3426bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mediator complex subunit 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGGAT CCAATGCAAA GTCCTCGGGG ACTGGCTTCG GCAGCCACAT TCCCAGCATC 
GAGGAAAAGA ACAAGCAGAT CCAGCAGGAA ACCATGATGG AGAAGCTGCG GGCCAAGTAC 
CGGAACAAGC CAAAGAGCTA CGAGGAGATC AAGAAGTCGG TGCGTATGTA CTTTATCGAG 
AAGCACTACC CTCTGGACCC CCTGTGCAAG GCCACGCTGC AGTCGGCGCT GGACAAGTTG 
CAGCACTACA TCAAGGTCAC GTCACGCCAC GGCCTTGTGG AGCGTTTGGA GAGCCTCTCG 
CGCCAGTTGG GCCTCAAGTT CATGGAGGAC CAGCAGCTTC TCTTCATATC AACGGACATG 
TTCTACGTAG AGATCCTGCT GGACGCCGCC GGCAGTCTGT CGGACGTCAA GGTGCACCAC 
GAGTGCAAGA TCGAGCAACA GTCCAGCGAG CTAGTGGCCT GCCTCAAGTC TGGCGACTTT 
GCAGACTTTA CGGTTCAACT CGAGGGTCTG TCCTCCATTT ACCAGCTGAA TGCCGAGCCC 
AAGGTGAAAA AGAAGGCGTT TGTAGCGCTT CAGGCCATGG AGACGGATAT CCAGAGCCTA 
TACCAACTGC ACCTGCAAGG TCACTCCGGA GACAGCTACT CACTTATGAC AAGTTCATCG 
GTGGGTCTTG TGCTGCCCCG TCGCGGTGGC CATCCTATGA GGCTTACTTA TTTCTGCCCG 
CCGCTCCACC TTCCCGAAGG GGATCCCAAA CTGGCCAGCG GCGATTTCAC CATCGACCAG 
GTAATGCGAA GCAGCTACGG ACTGAGTGCC ACTATCAATC TCGAAGGCTC ATCAGCCAAC 
AAACTGCAGA CTTTGCCAAC GGTGACTTTG GTCCGCGATG CCCAAACGGG GTTGGAGGTT 
CCCACCTATG CCCAACTTAA CCAGAACAAC TCGCTGCTGA TGCCGGCCAC GTTTGTGCTT 
CGGCTGAACA AACCCATGCC TGTTTGCCTG GAATCACTAA AGGCTCTCGG TCTGCCCGGT 
CTAGACTCAG TTGCCACTCC TCCTGGTCCG CCCACCACAG TGCTTAATCT CATAGTACAA 
ACTGCATCGA AGCAGGCCAT TAAGAACACA CAGCGCGGCC TGTATGTGAA TTTACCTAAA 
GAGACGCACT GCTATTTCTT TACCGACAAC CGCAAACTGC AGGGAACTCT GGTGTCATCA 
CTGCCCTTCA CTGAACCCGC CCAGGTTCCG CGAATCGTGG CGTTCCTCAA GAAACAAGCT 
CTCTTCTACA CCCTGCTCGC AAGCTGTGTT CGTGAGCAGC AGAAGCAGTA TAATGATATG 
GACAGCACTG TGATACTGGA GGTAACCGCC GTTTCATTTA ATCAAATCAC GGTTGAGCTG 
CAGCACCCGT ACGAAGAGTC GCTGGCCACT GTGGATTTTC TGCTTGAGGA TGGTCAGCCC 
ACGTGCAGCG TTTACTGTCT GACCAACGAA TACGAGCTGC TGTCCCAGAA GTTAACCCGC 
ACCGCGCGCA AAGTGGTATC CATTCCGATG GTAATCTACA AGTTGCTTAA GTGCTGGGAT 
GAGGAGCACG AATTCAAACT GCATGGCGCG ATTGGACCTG GATCTGGTTC TGGAGCAATC 
GGAGGTGGAG GCATGAGTGG TGGTGGACCT GTCTCAGGAG TAGGCAATAA CTTTAGCCAG 
TTTTCAATGG ATACGCCGAC GCCTTCCGAT GGAAGCCTAC CCGGAGGTGG CTTCGCCAAC 
ATTAACAACC TTAAAATGGA TGCGAAGTCG CGATCTCTAG CGGATGCTTT TGCCGCTTCT 
ACTTCGGCAG CAGCGGCCAT AGCGGGTTTG ATCAATCTTA AGCGGGAAAC CGATCCACAG 
TCTGGTAGTT CGGCCAGTGG CACCACGGTC AGCGGCAGCT CCAGCTCATC CGGCTCCGCC 
AAGACGAGTG ACCATGACAT TGCTGACAAG TACAAAAATA TCTGGAAGGA CAAGACTCCA 
AATTTGAAGC ATTGCGTCAG CATCACCCCC ATCCCAGGAG ATGGAAAGTC TGGATCAGCT 
GGGGGAGTGT CCGGGGTCGA GGTACAGCGA ACGGGTGGTA TCGAAATTAT TCCATTGAAT 
GCCCAGGCGG CTATAGCCGG AGGAGGTGTT ACAGCCTCAT CCAGTGCCAC TCCCACGACT 
ATAACTATTA CCCCAATAAC TGGCAAGGAT CCCAGTAAGG ACTCAACCAA AAAAAGTACC 
GCTGCTTCAG CTGGAGTAGG TGTGGCCGCA AAGCGTCCCC ACGAAAGTAG CACAAGTAGT 
TCGTCTACGT CCGGTTGTAG TGGTTCTGGC AGCTCCATGT CGTCTTCAGC CAGCAGCGGC 
TCTTCAGATA CGCAGAAGGA AAAGAAGCGC AAGAAAAAGC GCGACGATTC GCCCATGGGA 
CCGCCGGAGA AGATCTATTC TAGGCAAAAC TCGCCTGCAG GTGGCGCAGA TGCTTCCGCA 
ACTGGTGGAG TGGTTCGCAA GTTTTCCTCT CCCTCGTCGT CACCCAAAGC TGGTGGCGGT 
GGACAGGGTC TAATGGCCGG AGTGCCGACT GCCCGTCCCA GCCCCAAGCA CTCTCCCGTG 
TATAGCAGTC CCAAGCACAA CACCGCCTCC AACAGCCCGA AGTCGCCGTT TGGCACACAC 
TCACCCAAAC ACGGCTCTTC CGGAAAGCCG AGCATGTCAA CGCTTAAAAG TGCAGCTACA 
GCTGCCACCA TCTTAAGTCC CAAGGGCGAT AAGTCCTCGT CAGCTGTGGG TAACACATCT 
TCGGGACCAT CCGCCTCCTC AGGATCCAGC GGAGCTACTG GCCTGGTGAG GTCATTTGCC 
AGCGTTGGAG CTCCGCCTCC ACCACCTCCA ATTCCTCCAT TAGCGAGCTC GAGCGGATCT 
ATCAGCAGCA GCCAGAGTTT AAAGAAGGAG AAAACCTCTT CGGCATCCGG TTCCAGTTCC 
ACATCATCCT CGGCAACTGC AGGTGTCGCA TCTGGCGGAG GTATTTCACC GGCAAGTGTG 
GCTGCTGCAG TAGCTGCCCT GAAATCCTCA CAACAGCAGA TGAAGTCAGT CGCCAGTCTT 
TCGCATCTGG CGGCGGGCGG GGGACTGGGT AGCTATGCGG CCCCGTCGGG AGCGGGAGCA 
TCTGGTGCAG CCGCAGTGGT GGTGGGAGCA GGAGCTGGCG CTGGAGCGGG GGCCTCCGGA 
CTGGAGCTGA GTGCCCTGCG CAAGGGCATG GCGGGAGGTG CGTTTGATGA CGTCGACGGC 
AGCTTTAGCG CCAACAATTC CGGCACCAAC AACAACAGTG GCAGCGGGCT CGGCAGCGTC 
ATTGGTGTCT CCAGTCTCAG CGGTAGTGGG TCAGGGGCAG GAAACAGCGG GAGCAGCGGC 
AGCAGCAACG CTGGCAACAG CAACAATTCT GCAGCAGCAG CCACAGCCGG GAGCAGCGCC 
AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262183.1
CDS	152..3577
Translation	MSGSNAKSSGTGFGSHIPSIEEKNKQIQQETMMEKLRAKYRNKPKSYEEIKKSVRMYFIEKHYPLDPLCKATLQSALDKLQHYIKVTSRHGLVERLESLSRQLGLKFMEDQQLLFISTDMFYVEILLDAAGSLSDVKVHHECKIEQQSSELVACLKSGDFADFTVQLEGLSSIYQLNAEPKVKKKAFVALQAMETDIQSLYQLHLQGHSGDSYSLMTSSSVGLVLPRRGGHPMRLTYFCPPLHLPEGDPKLASGDFTIDQVMRSSYGLSATINLEGSSANKLQTLPTVTLVRDAQTGLEVPTYAQLNQNNSLLMPATFVLRLNKPMPVCLESLKALGLPGLDSVATPPGPPTTVLNLIVQTASKQAIKNTQRGLYVNLPKETHCYFFTDNRKLQGTLVSSLPFTEPAQVPRIVAFLKKQALFYTLLASCVREQQKQYNDMDSTVILEVTAVSFNQITVELQHPYEESLATVDFLLEDGQPTCSVYCLTNEYELLSQKLTRTARKVVSIPMVIYKLLKCWDEEHEFKLHGAIGPGSGSGAIGGGGMSGGGPVSGVGNNFSQFSMDTPTPSDGSLPGGGFANINNLKMDAKSRSLADAFAASTSAAAAIAGLINLKRETDPQSGSSASGTTVSGSSSSSGSAKTSDHDIADKYKNIWKDKTPNLKHCVSITPIPGDGKSGSAGGVSGVEVQRTGGIEIIPLNAQAAIAGGGVTASSSATPTTITITPITGKDPSKDSTKKSTAASAGVGVAAKRPHESSTSSSSTSGCSGSGSSMSSSASSGSSDTQKEKKRKKKRDDSPMGPPEKIYSRQNSPAGGADASATGGVVRKFSSPSSSPKAGGGGQGLMAGVPTARPSPKHSPVYSSPKHNTASNSPKSPFGTHSPKHGSSGKPSMSTLKSAATAATILSPKGDKSSSAVGNTSSGPSASSGSSGATGLVRSFASVGAPPPPPPIPPLASSSGSISSSQSLKKEKTSSASGSSSTSSSATAGVASGGGISPASVAAAVAALKSSQQQMKSVASLSHLAAGGGLGSYAAPSGAGASGAAAVVVGAGAGAGAGASGLELSALRKGMAGGAFDDVDGSFSANNSGTNNNSGSGLGSVIGVSSLSGSGSGAGNSGSSGSSNAGNSNNSAAAATAGSSAN

Target ORF information:

RefSeq Version	NM_001275254.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mediator complex subunit 1, transcript variant C (MED1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275254.1

ORF Insert Sequence:

ATGAGCGGAT CCAATGCAAA GTCCTCGGGG ACTGGCTTCG GCAGCCACAT TCCCAGCATC 
GAGGAAAAGA ACAAGCAGAT CCAGCAGGAA ACCATGATGG AGAAGCTGCG GGCCAAGTAC 
CGGAACAAGC CAAAGAGCTA CGAGGAGATC AAGAAGTCGG TGCGTATGTA CTTTATCGAG 
AAGCACTACC CTCTGGACCC CCTGTGCAAG GCCACGCTGC AGTCGGCGCT GGACAAGTTG 
CAGCACTACA TCAAGGTCAC GTCACGCCAC GGCCTTGTGG AGCGTTTGGA GAGCCTCTCG 
CGCCAGTTGG GCCTCAAGTT CATGGAGGAC CAGCAGCTTC TCTTCATATC AACGGACATG 
TTCTACGTAG AGATCCTGCT GGACGCCGCC GGCAGTCTGT CGGACGTCAA GGTGCACCAC 
GAGTGCAAGA TCGAGCAACA GTCCAGCGAG CTAGTGGCCT GCCTCAAGTC TGGCGACTTT 
GCAGACTTTA CGGTTCAACT CGAGGGTCTG TCCTCCATTT ACCAGCTGAA TGCCGAGCCC 
AAGGTGAAAA AGAAGGCGTT TGTAGCGCTT CAGGCCATGG AGACGGATAT CCAGAGCCTA 
TACCAACTGC ACCTGCAAGG TCACTCCGGA GACAGCTACT CACTTATGAC AAGTTCATCG 
GTGGGTCTTG TGCTGCCCCG TCGCGGTGGC CATCCTATGA GGCTTACTTA TTTCTGCCCG 
CCGCTCCACC TTCCCGAAGG GGATCCCAAA CTGGCCAGCG GCGATTTCAC CATCGACCAG 
GTAATGCGAA GCAGCTACGG ACTGAGTGCC ACTATCAATC TCGAAGGCTC ATCAGCCAAC 
AAACTGCAGA CTTTGCCAAC GGTGACTTTG GTCCGCGATG CCCAAACGGG GTTGGAGGTT 
CCCACCTATG CCCAACTTAA CCAGAACAAC TCGCTGCTGA TGCCGGCCAC GTTTGTGCTT 
CGGCTGAACA AACCCATGCC TGTTTGCCTG GAATCACTAA AGGCTCTCGG TCTGCCCGGT 
CTAGACTCAG TTGCCACTCC TCCTGGTCCG CCCACCACAG TGCTTAATCT CATAGTACAA 
ACTGCATCGA AGCAGGCCAT TAAGAACACA CAGCGCGGCC TGTATGTGAA TTTACCTAAA 
GAGACGCACT GCTATTTCTT TACCGACAAC CGCAAACTGC AGGGAACTCT GGTGTCATCA 
CTGCCCTTCA CTGAACCCGC CCAGGTTCCG CGAATCGTGG CGTTCCTCAA GAAACAAGCT 
CTCTTCTACA CCCTGCTCGC AAGCTGTGTT CGTGAGCAGC AGAAGCAGTA TAATGATATG 
GACAGCACTG TGATACTGGA GGTAACCGCC GTTTCATTTA ATCAAATCAC GGTTGAGCTG 
CAGCACCCGT ACGAAGAGTC GCTGGCCACT GTGGATTTTC TGCTTGAGGA TGGTCAGCCC 
ACGTGCAGCG TTTACTGTCT GACCAACGAA TACGAGCTGC TGTCCCAGAA GTTAACCCGC 
ACCGCGCGCA AAGTGGTATC CATTCCGATG GTAATCTACA AGTTGCTTAA GTGCTGGGAT 
GAGGAGCACG AATTCAAACT GCATGGCGCG ATTGGACCTG GATCTGGTTC TGGAGCAATC 
GGAGGTGGAG GCATGAGTGG TGGTGGACCT GTCTCAGGAG TAGGCAATAA CTTTAGCCAG 
TTTTCAATGG ATACGCCGAC GCCTTCCGAT GGAAGCCTAC CCGGAGGTGG CTTCGCCAAC 
ATTAACAACC TTAAAATGGA TGCGAAGTCG CGATCTCTAG CGGATGCTTT TGCCGCTTCT 
ACTTCGGCAG CAGCGGCCAT AGCGGGTTTG ATCAATCTTA AGCGGGAAAC CGATCCACAG 
TCTGGTAGTT CGGCCAGTGG CACCACGGTC AGCGGCAGCT CCAGCTCATC CGGCTCCGCC 
AAGACGAGTG ACCATGACAT TGCTGACAAG TACAAAAATA TCTGGAAGGA CAAGACTCCA 
AATTTGAAGC ATTGCGTCAG CATCACCCCC ATCCCAGGAG ATGGAAAGTC TGGATCAGCT 
GGGGGAGTGT CCGGGGTCGA GGTACAGCGA ACGGGTGGTA TCGAAATTAT TCCATTGAAT 
GCCCAGGCGG CTATAGCCGG AGGAGGTGTT ACAGCCTCAT CCAGTGCCAC TCCCACGACT 
ATAACTATTA CCCCAATAAC TGGCAAGGAT CCCAGTAAGG ACTCAACCAA AAAAAGTACC 
GCTGCTTCAG CTGGAGTAGG TGTGGCCGCA AAGCGTCCCC ACGAAAGTAG CACAAGTAGT 
TCGTCTACGT CCGGTTGTAG TGGTTCTGGC AGCTCCATGT CGTCTTCAGC CAGCAGCGGC 
TCTTCAGATA CGCAGAAGGA AAAGAAGCGC AAGAAAAAGC GCGACGATTC GCCCATGGGA 
CCGCCGGAGA AGATCTATTC TAGGCAAAAC TCGCCTGCAG GTGGCGCAGA TGCTTCCGCA 
ACTGGTGGAG TGGTTCGCAA GTTTTCCTCT CCCTCGTCGT CACCCAAAGC TGGTGGCGGT 
GGACAGGGTC TAATGGCCGG AGTGCCGACT GCCCGTCCCA GCCCCAAGCA CTCTCCCGTG 
TATAGCAGTC CCAAGCACAA CACCGCCTCC AACAGCCCGA AGTCGCCGTT TGGCACACAC 
TCACCCAAAC ACGGCTCTTC CGGAAAGCCG AGCATGTCAA CGCTTAAAAG TGCAGCTACA 
GCTGCCACCA TCTTAAGTCC CAAGGGCGAT AAGTCCTCGT CAGCTGTGGG TAACACATCT 
TCGGGACCAT CCGCCTCCTC AGGATCCAGC GGAGCTACTG GCCTGGTGAG GTCATTTGCC 
AGCGTTGGAG CTCCGCCTCC ACCACCTCCA ATTCCTCCAT TAGCGAGCTC GAGCGGATCT 
ATCAGCAGCA GCCAGAGTTT AAAGAAGGAG AAAACCTCTT CGGCATCCGG TTCCAGTTCC 
ACATCATCCT CGGCAACTGC AGGTGTCGCA TCTGGCGGAG GTATTTCACC GGCAAGTGTG 
GCTGCTGCAG TAGCTGCCCT GAAATCCTCA CAACAGCAGA TGAAGTCAGT CGCCAGTCTT 
TCGCATCTGG CGGCGGGCGG GGGACTGGGT AGCTATGCGG CCCCGTCGGG AGCGGGAGCA 
TCTGGTGCAG CCGCAGTGGT GGTGGGAGCA GGAGCTGGCG CTGGAGCGGG GGCCTCCGGA 
CTGGAGCTGA GTGCCCTGCG CAAGGGCATG GCGGGAGGTG CGTTTGATGA CGTCGACGGC 
AGCTTTAGCG CCAACAATTC CGGCACCAAC AACAACAGTG GCAGCGGGCT CGGCAGCGTC 
ATTGGTGTCT CCAGTCTCAG CGGTAGTGGG TCAGGGGCAG GAAACAGCGG GAGCAGCGGC 
AGCAGCAACG CTGGCAACAG CAACAATTCT GCAGCAGCAG CCACAGCCGG GAGCAGCGCC 
AACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED1 ( NM_001202224.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189153.1 Drosophila melanogaster mediator complex subunit 1, transcript variant B (MED1), mRNA.

Price & Availability↑

CloneID	OFa19227
Clone ID Related Accession (Same CDS sequence)	NM_001202224.2
Accession Version	NM_001202224.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mediator complex subunit 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001202224.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGGAT CCAATGCAAA GTCCTCGGGG ACTGGCTTCG GCAGCCACAT TCCCAGCATC 
GAGGAAAAGA ACAAGCAGAT CCAGCAGGAA ACCATGATGG AGAAGCTGCG GGCCAAGTAC 
CGGAACAAGC CAAAGAGCTA CGAGGAGATC AAGAAGTCGG TGCGTATGTA CTTTATCGAG 
AAGCACTACC CTCTGGACCC CCTGTGCAAG GCCACGCTGC AGTCGGCGCT GGACAAGTTG 
CAGCACTACA TCAAGGTCAC GTCACGCCAC GGCCTTGTGG AGCGTTTGGA GAGCCTCTCG 
CGCCAGTTGG GCCTCAAGTT CATGGAGGAC CAGCAGCTTC TCTTCATATC AACGGACATG 
TTCTACGTAG AGATCCTGCT GGACGCCGCC GGCAGTCTGT CGGACGTCAA GGTGCACCAC 
GAGTGCAAGA TCGAGCAACA GTCCAGCGAG CTAGTGGCCT GCCTCAAGTC TGGCGACTTT 
GCAGACTTTA CGGTTCAACT CGAGGGTCTG TCCTCCATTT ACCAGCTGAA TGCCGAGCCC 
AAGGTGAAAA AGAAGGCGTT TGTAGCGCTT CAGGCCATGG AGACGGATAT CCAGAGCCTA 
TACCAACTGC ACCTGCAAGG TCACTCCGGA GACAGCTACT CACTTATGAC AAGTTCATCG 
GTGGGTCTTG TGCTGCCCCG TCGCGGTGGC CATCCTATGA GGCTTACTTA TTTCTGCCCG 
CCGCTCCACC TTCCCGAAGG GGATCCCAAA CTGGCCAGCG GCGATTTCAC CATCGACCAG 
GTAATGCGAA GCAGCTACGG ACTGAGTGCC ACTATCAATC TCGAAGGCTC ATCAGCCAAC 
AAACTGCAGA CTTTGCCAAC GGTGACTTTG GTCCGCGATG CCCAAACGGG GTTGGAGGTT 
CCCACCTATG CCCAACTTAA CCAGAACAAC TCGCTGCTGA TGCCGGCCAC GTTTGTGCTT 
CGGCTGAACA AACCCATGCC TGTTTGCCTG GAATCACTAA AGGCTCTCGG TCTGCCCGGT 
CTAGACTCAG TTGCCACTCC TCCTGGTCCG CCCACCACAG TGCTTAATCT CATAGTACAA 
ACTGCATCGA AGCAGGCCAT TAAGAACACA CAGCGCGGCC TGTATGTGAA TTTACCTAAA 
GAGACGCACT GCTATTTCTT TACCGACAAC CGCAAACTGC AGGGAACTCT GGTGTCATCA 
CTGCCCTTCA CTGAACCCGC CCAGGTTCCG CGAATCGTGG CGTTCCTCAA GAAACAAGCT 
CTCTTCTACA CCCTGCTCGC AAGCTGTGTT CGTGAGCAGC AGAAGCAGTA TAATGATATG 
GACAGCACTG TGATACTGGA GGTAACCGCC GTTTCATTTA ATCAAATCAC GGTTGAGCTG 
CAGCACCCGT ACGAAGAGTC GCTGGCCACT GTGGATTTTC TGCTTGAGGA TGGTCAGCCC 
ACGTGCAGCG TTTACTGTCT GACCAACGAA TACGAGCTGC TGTCCCAGAA GTTAACCCGC 
ACCGCGCGCA AAGTGGTATC CATTCCGATG GTAATCTACA AGTTGCTTAA GTGCTGGGAT 
GAGGAGCACG AATTCAAACT GCATGGCGCG ATTGGACCTG GATCTGGTTC TGGAGCAATC 
GGAGGTGGAG GCATGAGTGG TGGTGGACCT GTCTCAGGAG TAGGCAATAA CTTTAGCCAG 
TTTTCAATGG ATACGCCGAC GCCTTCCGAT GGAAGCCTAC CCGGAGGTGG CTTCGCCAAC 
ATTAACAACC TTAAAATGGA TGCGAAGTCG CGATCTCTAG CGGATGCTTT TGCCGCTTCT 
ACTTCGGCAG CAGCGGCCAT AGCGGGTTTG ATCAATCTTA AGCGGGAAAC CGATCCACAG 
TCTGGTAGTT CGGCCAGTGG CACCACGGTC AGCGGCAGCT CCAGCTCATC CGGCTCCGCC 
AAGACGAGTG ACCATGACAT TGCTGACAAG TACAAAAATA TCTGGAAGGA CAAGACTCCA 
AATTTGAAGC ATTGCGTCAG CATCACCCCC ATCCCAGGAG ATGGAAAGTC TGGATCAGCT 
GGGGGAGTGT CCGGGGTCGA GGTACAGCGA ACGGGTGGTA TCGAAATTAT TCCATTGAAT 
GCCCAGGCGG CTATAGCCGG AGGAGGTGTT ACAGCCTCAT CCAGTGCCAC TCCCACGACT 
ATAACTATTA CCCCAATAAC TGGCAAGGAT CCCAGTAAGG ACTCAACCAA AAAAAGTACC 
GCTGCTTCAG CTGGAGTAGG TGTGGCCGCA AAGCGTCCCC ACGAAAGTAG CACAAGTAGT 
TCGTCTACGT CCGGTTGTAG TGGTTCTGGC AGCTCCATGT CGTCTTCAGC CAGCAGCGGC 
TCTTCAGATA CGCAGAAGGA AAAGAAGCGC AAGAAAAAGC GCGACGATTC GCCCATGGGA 
CCGCCGGAGA AGATCTATTC TAGGCAAAAC TCGCCTGCAG GTGGCGCAGA TGCTTCCGCA 
ACTGGTGGAG TGGTTCGCAA GTTTTCCTCT CCCTCGTCGT CACCCAAAGC TGGTGGCGGT 
GGACAGGGTC TAATGGCCGG AGTGCCGACT GCCCGTCCCA GCCCCAAGCA CTCTCCCGTG 
TATAGCAGTC CCAAGCACAA CACCGCCTCC AACAGCCCGA AGTCGCCGTT TGGCACACAC 
TCACCCAAAC ACGGCTCTTC CGGAAAGCCG AGCATGTCAA CGCTTAAAAG TGCAGCTACA 
GCTGCCACCA TCTTAAGTCC CAAGGGCGAT AAGTCCTCGT CAGCTGTGGG TAACACATCT 
TCGGGACCAT CCGCCTCCTC AGGATCCAGC GGAGCTACTG GCCTGGTGAG GTCATTTGCC 
AGCGTTGGAG CTCCGCCTCC ACCACCTCCA ATTCCTCCAT TAGCGAGCTC GAGCGGATCT 
ATCAGCAGCA GCCAGAGTTT AAAGAAGGAG AAAACCTCTT CGGCATCCGG TTCCAGTTCC 
ACATCATCCT CGGCAACTGC AGGTGTCGCA TCTGGCGGAG GTATTTCACC GGCAAGTGTG 
GCTGCTGCAG TAGCTGCCCT GAAATCCTCA CAACAGCAGA TGAAGTCAGT CGCCAGTCTT 
TCGCATCTGG CGGCGGGCGG GGGACTGGGT AGCTATGCGG CCCCGTCGGG AGCGGGAGCA 
TCTGGTGCAG CCGCAGTGGT GGTGGGAGCA GGAGCTGGCG CTGGAGCGGG GGCCTCCGGA 
CTGGAGCTGA GTGCCCTGCG CAAGGGCATG GCGGGAGGTG CGGTAAGTCC GTTTTCATTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189153.1
CDS	152..3394
Translation	MSGSNAKSSGTGFGSHIPSIEEKNKQIQQETMMEKLRAKYRNKPKSYEEIKKSVRMYFIEKHYPLDPLCKATLQSALDKLQHYIKVTSRHGLVERLESLSRQLGLKFMEDQQLLFISTDMFYVEILLDAAGSLSDVKVHHECKIEQQSSELVACLKSGDFADFTVQLEGLSSIYQLNAEPKVKKKAFVALQAMETDIQSLYQLHLQGHSGDSYSLMTSSSVGLVLPRRGGHPMRLTYFCPPLHLPEGDPKLASGDFTIDQVMRSSYGLSATINLEGSSANKLQTLPTVTLVRDAQTGLEVPTYAQLNQNNSLLMPATFVLRLNKPMPVCLESLKALGLPGLDSVATPPGPPTTVLNLIVQTASKQAIKNTQRGLYVNLPKETHCYFFTDNRKLQGTLVSSLPFTEPAQVPRIVAFLKKQALFYTLLASCVREQQKQYNDMDSTVILEVTAVSFNQITVELQHPYEESLATVDFLLEDGQPTCSVYCLTNEYELLSQKLTRTARKVVSIPMVIYKLLKCWDEEHEFKLHGAIGPGSGSGAIGGGGMSGGGPVSGVGNNFSQFSMDTPTPSDGSLPGGGFANINNLKMDAKSRSLADAFAASTSAAAAIAGLINLKRETDPQSGSSASGTTVSGSSSSSGSAKTSDHDIADKYKNIWKDKTPNLKHCVSITPIPGDGKSGSAGGVSGVEVQRTGGIEIIPLNAQAAIAGGGVTASSSATPTTITITPITGKDPSKDSTKKSTAASAGVGVAAKRPHESSTSSSSTSGCSGSGSSMSSSASSGSSDTQKEKKRKKKRDDSPMGPPEKIYSRQNSPAGGADASATGGVVRKFSSPSSSPKAGGGGQGLMAGVPTARPSPKHSPVYSSPKHNTASNSPKSPFGTHSPKHGSSGKPSMSTLKSAATAATILSPKGDKSSSAVGNTSSGPSASSGSSGATGLVRSFASVGAPPPPPPIPPLASSSGSISSSQSLKKEKTSSASGSSSTSSSATAGVASGGGISPASVAAAVAALKSSQQQMKSVASLSHLAAGGGLGSYAAPSGAGASGAAAVVVGAGAGAGAGASGLELSALRKGMAGGAVSPFSL

Target ORF information:

RefSeq Version	NM_001202224.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mediator complex subunit 1, transcript variant B (MED1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202224.2

ORF Insert Sequence:

ATGAGCGGAT CCAATGCAAA GTCCTCGGGG ACTGGCTTCG GCAGCCACAT TCCCAGCATC 
GAGGAAAAGA ACAAGCAGAT CCAGCAGGAA ACCATGATGG AGAAGCTGCG GGCCAAGTAC 
CGGAACAAGC CAAAGAGCTA CGAGGAGATC AAGAAGTCGG TGCGTATGTA CTTTATCGAG 
AAGCACTACC CTCTGGACCC CCTGTGCAAG GCCACGCTGC AGTCGGCGCT GGACAAGTTG 
CAGCACTACA TCAAGGTCAC GTCACGCCAC GGCCTTGTGG AGCGTTTGGA GAGCCTCTCG 
CGCCAGTTGG GCCTCAAGTT CATGGAGGAC CAGCAGCTTC TCTTCATATC AACGGACATG 
TTCTACGTAG AGATCCTGCT GGACGCCGCC GGCAGTCTGT CGGACGTCAA GGTGCACCAC 
GAGTGCAAGA TCGAGCAACA GTCCAGCGAG CTAGTGGCCT GCCTCAAGTC TGGCGACTTT 
GCAGACTTTA CGGTTCAACT CGAGGGTCTG TCCTCCATTT ACCAGCTGAA TGCCGAGCCC 
AAGGTGAAAA AGAAGGCGTT TGTAGCGCTT CAGGCCATGG AGACGGATAT CCAGAGCCTA 
TACCAACTGC ACCTGCAAGG TCACTCCGGA GACAGCTACT CACTTATGAC AAGTTCATCG 
GTGGGTCTTG TGCTGCCCCG TCGCGGTGGC CATCCTATGA GGCTTACTTA TTTCTGCCCG 
CCGCTCCACC TTCCCGAAGG GGATCCCAAA CTGGCCAGCG GCGATTTCAC CATCGACCAG 
GTAATGCGAA GCAGCTACGG ACTGAGTGCC ACTATCAATC TCGAAGGCTC ATCAGCCAAC 
AAACTGCAGA CTTTGCCAAC GGTGACTTTG GTCCGCGATG CCCAAACGGG GTTGGAGGTT 
CCCACCTATG CCCAACTTAA CCAGAACAAC TCGCTGCTGA TGCCGGCCAC GTTTGTGCTT 
CGGCTGAACA AACCCATGCC TGTTTGCCTG GAATCACTAA AGGCTCTCGG TCTGCCCGGT 
CTAGACTCAG TTGCCACTCC TCCTGGTCCG CCCACCACAG TGCTTAATCT CATAGTACAA 
ACTGCATCGA AGCAGGCCAT TAAGAACACA CAGCGCGGCC TGTATGTGAA TTTACCTAAA 
GAGACGCACT GCTATTTCTT TACCGACAAC CGCAAACTGC AGGGAACTCT GGTGTCATCA 
CTGCCCTTCA CTGAACCCGC CCAGGTTCCG CGAATCGTGG CGTTCCTCAA GAAACAAGCT 
CTCTTCTACA CCCTGCTCGC AAGCTGTGTT CGTGAGCAGC AGAAGCAGTA TAATGATATG 
GACAGCACTG TGATACTGGA GGTAACCGCC GTTTCATTTA ATCAAATCAC GGTTGAGCTG 
CAGCACCCGT ACGAAGAGTC GCTGGCCACT GTGGATTTTC TGCTTGAGGA TGGTCAGCCC 
ACGTGCAGCG TTTACTGTCT GACCAACGAA TACGAGCTGC TGTCCCAGAA GTTAACCCGC 
ACCGCGCGCA AAGTGGTATC CATTCCGATG GTAATCTACA AGTTGCTTAA GTGCTGGGAT 
GAGGAGCACG AATTCAAACT GCATGGCGCG ATTGGACCTG GATCTGGTTC TGGAGCAATC 
GGAGGTGGAG GCATGAGTGG TGGTGGACCT GTCTCAGGAG TAGGCAATAA CTTTAGCCAG 
TTTTCAATGG ATACGCCGAC GCCTTCCGAT GGAAGCCTAC CCGGAGGTGG CTTCGCCAAC 
ATTAACAACC TTAAAATGGA TGCGAAGTCG CGATCTCTAG CGGATGCTTT TGCCGCTTCT 
ACTTCGGCAG CAGCGGCCAT AGCGGGTTTG ATCAATCTTA AGCGGGAAAC CGATCCACAG 
TCTGGTAGTT CGGCCAGTGG CACCACGGTC AGCGGCAGCT CCAGCTCATC CGGCTCCGCC 
AAGACGAGTG ACCATGACAT TGCTGACAAG TACAAAAATA TCTGGAAGGA CAAGACTCCA 
AATTTGAAGC ATTGCGTCAG CATCACCCCC ATCCCAGGAG ATGGAAAGTC TGGATCAGCT 
GGGGGAGTGT CCGGGGTCGA GGTACAGCGA ACGGGTGGTA TCGAAATTAT TCCATTGAAT 
GCCCAGGCGG CTATAGCCGG AGGAGGTGTT ACAGCCTCAT CCAGTGCCAC TCCCACGACT 
ATAACTATTA CCCCAATAAC TGGCAAGGAT CCCAGTAAGG ACTCAACCAA AAAAAGTACC 
GCTGCTTCAG CTGGAGTAGG TGTGGCCGCA AAGCGTCCCC ACGAAAGTAG CACAAGTAGT 
TCGTCTACGT CCGGTTGTAG TGGTTCTGGC AGCTCCATGT CGTCTTCAGC CAGCAGCGGC 
TCTTCAGATA CGCAGAAGGA AAAGAAGCGC AAGAAAAAGC GCGACGATTC GCCCATGGGA 
CCGCCGGAGA AGATCTATTC TAGGCAAAAC TCGCCTGCAG GTGGCGCAGA TGCTTCCGCA 
ACTGGTGGAG TGGTTCGCAA GTTTTCCTCT CCCTCGTCGT CACCCAAAGC TGGTGGCGGT 
GGACAGGGTC TAATGGCCGG AGTGCCGACT GCCCGTCCCA GCCCCAAGCA CTCTCCCGTG 
TATAGCAGTC CCAAGCACAA CACCGCCTCC AACAGCCCGA AGTCGCCGTT TGGCACACAC 
TCACCCAAAC ACGGCTCTTC CGGAAAGCCG AGCATGTCAA CGCTTAAAAG TGCAGCTACA 
GCTGCCACCA TCTTAAGTCC CAAGGGCGAT AAGTCCTCGT CAGCTGTGGG TAACACATCT 
TCGGGACCAT CCGCCTCCTC AGGATCCAGC GGAGCTACTG GCCTGGTGAG GTCATTTGCC 
AGCGTTGGAG CTCCGCCTCC ACCACCTCCA ATTCCTCCAT TAGCGAGCTC GAGCGGATCT 
ATCAGCAGCA GCCAGAGTTT AAAGAAGGAG AAAACCTCTT CGGCATCCGG TTCCAGTTCC 
ACATCATCCT CGGCAACTGC AGGTGTCGCA TCTGGCGGAG GTATTTCACC GGCAAGTGTG 
GCTGCTGCAG TAGCTGCCCT GAAATCCTCA CAACAGCAGA TGAAGTCAGT CGCCAGTCTT 
TCGCATCTGG CGGCGGGCGG GGGACTGGGT AGCTATGCGG CCCCGTCGGG AGCGGGAGCA 
TCTGGTGCAG CCGCAGTGGT GGTGGGAGCA GGAGCTGGCG CTGGAGCGGG GGCCTCCGGA 
CTGGAGCTGA GTGCCCTGCG CAAGGGCATG GCGGGAGGTG CGGTAAGTCC GTTTTCATTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED1 ( NM_141084.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_649341.1 Drosophila melanogaster mediator complex subunit 1, transcript variant A (MED1), mRNA.

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CloneID	OFa21195
Clone ID Related Accession (Same CDS sequence)	NM_141084.3
Accession Version	NM_141084.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mediator complex subunit 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_141084.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGGAT CCAATGCAAA GTCCTCGGGG ACTGGCTTCG GCAGCCACAT TCCCAGCATC 
GAGGAAAAGA ACAAGCAGAT CCAGCAGGAA ACCATGATGG AGAAGCTGCG GGCCAAGTAC 
CGGAACAAGC CAAAGAGCTA CGAGGAGATC AAGAAGTCGG TGCGTATGTA CTTTATCGAG 
AAGCACTACC CTCTGGACCC CCTGTGCAAG GCCACGCTGC AGTCGGCGCT GGACAAGTTG 
CAGCACTACA TCAAGGTCAC GTCACGCCAC GGCCTTGTGG AGCGTTTGGA GAGCCTCTCG 
CGCCAGTTGG GCCTCAAGTT CATGGAGGAC CAGCAGCTTC TCTTCATATC AACGGACATG 
TTCTACGTAG AGATCCTGCT GGACGCCGCC GGCAGTCTGT CGGACGTCAA GGTGCACCAC 
GAGTGCAAGA TCGAGCAACA GTCCAGCGAG CTAGTGGCCT GCCTCAAGTC TGGCGACTTT 
GCAGACTTTA CGGTTCAACT CGAGGGTCTG TCCTCCATTT ACCAGCTGAA TGCCGAGCCC 
AAGGTGAAAA AGAAGGCGTT TGTAGCGCTT CAGGCCATGG AGACGGATAT CCAGAGCCTA 
TACCAACTGC ACCTGCAAGG TCACTCCGGA GACAGCTACT CACTTATGAC AAGTTCATCG 
GTGGGTCTTG TGCTGCCCCG TCGCGGTGGC CATCCTATGA GGCTTACTTA TTTCTGCCCG 
CCGCTCCACC TTCCCGAAGG GGATCCCAAA CTGGCCAGCG GCGATTTCAC CATCGACCAG 
GTAATGCGAA GCAGCTACGG ACTGAGTGCC ACTATCAATC TCGAAGGCTC ATCAGCCAAC 
AAACTGCAGA CTTTGCCAAC GGTGACTTTG GTCCGCGATG CCCAAACGGG GTTGGAGGTT 
CCCACCTATG CCCAACTTAA CCAGAACAAC TCGCTGCTGA TGCCGGCCAC GTTTGTGCTT 
CGGCTGAACA AACCCATGCC TGTTTGCCTG GAATCACTAA AGGCTCTCGG TCTGCCCGGT 
CTAGACTCAG TTGCCACTCC TCCTGGTCCG CCCACCACAG TGCTTAATCT CATAGTACAA 
ACTGCATCGA AGCAGGCCAT TAAGAACACA CAGCGCGGCC TGTATGTGAA TTTACCTAAA 
GAGACGCACT GCTATTTCTT TACCGACAAC CGCAAACTGC AGGGAACTCT GGTGTCATCA 
CTGCCCTTCA CTGAACCCGC CCAGGTTCCG CGAATCGTGG CGTTCCTCAA GAAACAAGCT 
CTCTTCTACA CCCTGCTCGC AAGCTGTGTT CGTGAGCAGC AGAAGCAGTA TAATGATATG 
GACAGCACTG TGATACTGGA GGTAACCGCC GTTTCATTTA ATCAAATCAC GGTTGAGCTG 
CAGCACCCGT ACGAAGAGTC GCTGGCCACT GTGGATTTTC TGCTTGAGGA TGGTCAGCCC 
ACGTGCAGCG TTTACTGTCT GACCAACGAA TACGAGCTGC TGTCCCAGAA GTTAACCCGC 
ACCGCGCGCA AAGTGGTATC CATTCCGATG GTAATCTACA AGTTGCTTAA GTGCTGGGAT 
GAGGAGCACG AATTCAAACT GCATGGCGCG ATTGGACCTG GATCTGGTTC TGGAGCAATC 
GGAGGTGGAG GCATGAGTGG TGGTGGACCT GTCTCAGGAG TAGGCAATAA CTTTAGCCAG 
TTTTCAATGG ATACGCCGAC GCCTTCCGAT GGAAGCCTAC CCGGAGGTGG CTTCGCCAAC 
ATTAACAACC TTAAAATGGA TGCGAAGTCG CGATCTCTAG CGGATGCTTT TGCCGCTTCT 
ACTTCGGCAG CAGCGGCCAT AGCGGGTTTG ATCAATCTTA AGCGGGAAAC CGATCCACAG 
TCTGGTAGTT CGGCCAGTGG CACCACGGTC AGCGGCAGCT CCAGCTCATC CGGCTCCGCC 
AAGACGAGTG ACCATGACAT TGCTGACAAG TACAAAAATA TCTGGAAGGA CAAGACTCCA 
AATTTGAAGC ATTGCGTCAG CATCACCCCC ATCCCAGGAG ATGGAAAGTC TGGATCAGCT 
GGGGGAGTGT CCGGGGTCGA GGTACAGCGA ACGGGTGGTA TCGAAATTAT TCCATTGAAT 
GCCCAGGCGG CTATAGCCGG AGGAGGTGTT ACAGCCTCAT CCAGTGCCAC TCCCACGACT 
ATAACTATTA CCCCAATAAC TGGCAAGGAT CCCAGTAAGG ACTCAACCAA AAAAAGTACC 
GCTGCTTCAG CTGGAGTAGG TGTGGCCGCA AAGCGTCCCC ACGAAAGTAG CACAAGTAGT 
TCGTCTACGT CCGGTTGTAG TGGTTCTGGC AGCTCCATGT CGTCTTCAGC CAGCAGCGGC 
TCTTCAGATA CGCAGAAGGA AAAGAAGCGC AAGAAAAAGC GCGACGATTC GCCCATGGGA 
CCGCCGGAGA AGATCTATTC TAGGCAAAAC TCGCCTGCAG GTGGCGCAGA TGCTTCCGCA 
ACTGGTGGAG TGGTTCGCAA GTTTTCCTCT CCCTCGTCGT CACCCAAAGC TGGTGGCGGT 
GGACAGGGTC TAATGGCCGG AGTGCCGACT GCCCGTCCCA GCCCCAAGCA CTCTCCCGTG 
TATAGCAGTC CCAAGCACAA CACCGCCTCC AACAGCCCGA AGTCGCCGTT TGGCACACAC 
TCACCCAAAC ACGGCTCTTC CGGAAAGCCG AGCATGTCAA CGCTTAAAAG TGCAGCTACA 
GCTGCCACCA TCTTAAGTCC CAAGGGCGAT AAGTCCTCGT CAGCTGTGGG TAACACATCT 
TCGGGACCAT CCGCCTCCTC AGGATCCAGC GGAGCTACTG GCCTGGTGAG GTCATTTGCC 
AGCGTTGGAG CTCCGCCTCC ACCACCTCCA ATTCCTCCAT TAGCGAGCTC GAGCGGATCT 
ATCAGCAGCA GCCAGAGTTT AAAGAAGGAG AAAACCTCTT CGGCATCCGG TTCCAGTTCC 
ACATCATCCT CGGCAACTGC AGGTGTCGCA TCTGGCGGAG GTATTTCACC GGCAAGTGTG 
GCTGCTGCAG TAGCTGCCCT GAAATCCTCA CAACAGCAGA TGAAGTCAGT CGCCAGTCTT 
TCGCATCTGG CGGCGGGCGG GGGACTGGGT AGCTATGCGG CCCCGTCGGG AGCGGGAGCA 
TCTGGTGCAG CCGCAGTGGT GGTGGGAGCA GGAGCTGGCG CTGGAGCGGG GGCCTCCGGA 
CTGGAGCTGA GTGCCCTGCG CAAGGGCATG GCGGGAGGTG CGGTTAGTTT GATGACGTCG 
ACGGCAGCTT TAGCGCCAAC AATTCCGGCA CCAACAACAA CAGTGGCAGC GGGCTCGGCA 
GCGTCATTGG TGTCTCCAGT CTCAGCGGTA GTGGGTCAGG GGCAGGAAAC AGCGGGAGCA 
GCGGCAGCAG CAACGCTGGC AACAGCAACA ATTCTGCAGC AGCAGCCACA GCCGGGAGCA 
GCGCCAACTA GTAGCTGCTT AACCACCAGC GGGGGCAGCA GCGACTCCGC CGGGAGCATC 
AATCCGGCCG GAGCCTCCAC GGAATACATG GTGAAGCCCA GCAGCCAAGA GGGCCTCAAG 
CTGACCATCA ACAAGACGGG AAGCAGCAAG AGCTCCGGCA CCGGCTCCGG CTCTAGTTCC 
TCCTCTGGCC TCCAAGCGAA GGCAAAGAGC AGCAGCAGCG GCGCCACGAG CTTCGCTGGG 
TCCACTGGAT CCACGAAGAA GCAGCATACC GGACTCAAGC CGGGCGTTAA CAGCGGACCC 
GCTTCCAAGA AGGCCACCGC AGCGGTCTCG TCCGCCACGG CATCATCCAG CAAGCATTTC 
TTCCAGAAGG CAAACTCCTC GGGCAATCTC AGCAGCAAGC TCAGTGGGTC GGGATCAGGT 
GGCGGAATTC CGCTGACTAA GAGCAACAGC ACTAACTCCT TCCAAGAGCA CAATGCCCCC 
AGGCGGCGTC CCAGCATGGG AGCCTTGGCT AGCGGCAGCA GTGGAGGAGG CAGCGGGCAG 
CGAAAGTTAG GCTCCGCTTC GGGAGGAGGC AGCGGATCTT CGGGGTCGGT ATCGCCGGCC 
CTTAGCGGCT CAATGTCGCA GCCACCGCCG CGATTCGATC ACCACACGGA TATGATGACC 
ATCCTCCAAT ACGCTTCTCC GACAATGGCC GCAAGCATGG AAGGCTTCAT CAAGGGGCTC 
CACAACAAAT TCCAGATCCC AAAGTTATCG CAGAGAGGAA GCGGAGGCAA TACAACCAGT 
GGACGCAGCA CGCCAAGTGG CAGTTCAGAA CCGGCTTTAG CGGGAACATC ATCTTCGATA 
TTAGGACCTA TTGCCAGCTC TACTGGACTG ACGGAGCCGG AAGCGAAGCC GCCGGTGCCT 
CCTTCGCAGT CTGGCAATGA AGGACTGCTC AATCTTAGCA GCACGGCGGG TACACCATCG 
GCGGATGGGA TAGATGAGGA GCTTTTGGCT AGCCTGGCTG GCGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_649341.1
CDS	152..4579
Translation	MSGSNAKSSGTGFGSHIPSIEEKNKQIQQETMMEKLRAKYRNKPKSYEEIKKSVRMYFIEKHYPLDPLCKATLQSALDKLQHYIKVTSRHGLVERLESLSRQLGLKFMEDQQLLFISTDMFYVEILLDAAGSLSDVKVHHECKIEQQSSELVACLKSGDFADFTVQLEGLSSIYQLNAEPKVKKKAFVALQAMETDIQSLYQLHLQGHSGDSYSLMTSSSVGLVLPRRGGHPMRLTYFCPPLHLPEGDPKLASGDFTIDQVMRSSYGLSATINLEGSSANKLQTLPTVTLVRDAQTGLEVPTYAQLNQNNSLLMPATFVLRLNKPMPVCLESLKALGLPGLDSVATPPGPPTTVLNLIVQTASKQAIKNTQRGLYVNLPKETHCYFFTDNRKLQGTLVSSLPFTEPAQVPRIVAFLKKQALFYTLLASCVREQQKQYNDMDSTVILEVTAVSFNQITVELQHPYEESLATVDFLLEDGQPTCSVYCLTNEYELLSQKLTRTARKVVSIPMVIYKLLKCWDEEHEFKLHGAIGPGSGSGAIGGGGMSGGGPVSGVGNNFSQFSMDTPTPSDGSLPGGGFANINNLKMDAKSRSLADAFAASTSAAAAIAGLINLKRETDPQSGSSASGTTVSGSSSSSGSAKTSDHDIADKYKNIWKDKTPNLKHCVSITPIPGDGKSGSAGGVSGVEVQRTGGIEIIPLNAQAAIAGGGVTASSSATPTTITITPITGKDPSKDSTKKSTAASAGVGVAAKRPHESSTSSSSTSGCSGSGSSMSSSASSGSSDTQKEKKRKKKRDDSPMGPPEKIYSRQNSPAGGADASATGGVVRKFSSPSSSPKAGGGGQGLMAGVPTARPSPKHSPVYSSPKHNTASNSPKSPFGTHSPKHGSSGKPSMSTLKSAATAATILSPKGDKSSSAVGNTSSGPSASSGSSGATGLVRSFASVGAPPPPPPIPPLASSSGSISSSQSLKKEKTSSASGSSSTSSSATAGVASGGGISPASVAAAVAALKSSQQQMKSVASLSHLAAGGGLGSYAAPSGAGASGAAAVVVGAGAGAGAGASGLELSALRKGMAGGAVSLMTSTAALAPTIPAPTTTVAAGSAASLVSPVSAVVGQGQETAGAAAAATLATATILQQQPQPGAAPTSSCLTTSGGSSDSAGSINPAGASTEYMVKPSSQEGLKLTINKTGSSKSSGTGSGSSSSSGLQAKAKSSSSGATSFAGSTGSTKKQHTGLKPGVNSGPASKKATAAVSSATASSSKHFFQKANSSGNLSSKLSGSGSGGGIPLTKSNSTNSFQEHNAPRRRPSMGALASGSSGGGSGQRKLGSASGGGSGSSGSVSPALSGSMSQPPPRFDHHTDMMTILQYASPTMAASMEGFIKGLHNKFQIPKLSQRGSGGNTTSGRSTPSGSSEPALAGTSSSILGPIASSTGLTEPEAKPPVPPSQSGNEGLLNLSSTAGTPSADGIDEELLASLAGE

Target ORF information:

RefSeq Version	NM_141084.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mediator complex subunit 1, transcript variant A (MED1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_141084.3

ORF Insert Sequence:

ATGAGCGGAT CCAATGCAAA GTCCTCGGGG ACTGGCTTCG GCAGCCACAT TCCCAGCATC 
GAGGAAAAGA ACAAGCAGAT CCAGCAGGAA ACCATGATGG AGAAGCTGCG GGCCAAGTAC 
CGGAACAAGC CAAAGAGCTA CGAGGAGATC AAGAAGTCGG TGCGTATGTA CTTTATCGAG 
AAGCACTACC CTCTGGACCC CCTGTGCAAG GCCACGCTGC AGTCGGCGCT GGACAAGTTG 
CAGCACTACA TCAAGGTCAC GTCACGCCAC GGCCTTGTGG AGCGTTTGGA GAGCCTCTCG 
CGCCAGTTGG GCCTCAAGTT CATGGAGGAC CAGCAGCTTC TCTTCATATC AACGGACATG 
TTCTACGTAG AGATCCTGCT GGACGCCGCC GGCAGTCTGT CGGACGTCAA GGTGCACCAC 
GAGTGCAAGA TCGAGCAACA GTCCAGCGAG CTAGTGGCCT GCCTCAAGTC TGGCGACTTT 
GCAGACTTTA CGGTTCAACT CGAGGGTCTG TCCTCCATTT ACCAGCTGAA TGCCGAGCCC 
AAGGTGAAAA AGAAGGCGTT TGTAGCGCTT CAGGCCATGG AGACGGATAT CCAGAGCCTA 
TACCAACTGC ACCTGCAAGG TCACTCCGGA GACAGCTACT CACTTATGAC AAGTTCATCG 
GTGGGTCTTG TGCTGCCCCG TCGCGGTGGC CATCCTATGA GGCTTACTTA TTTCTGCCCG 
CCGCTCCACC TTCCCGAAGG GGATCCCAAA CTGGCCAGCG GCGATTTCAC CATCGACCAG 
GTAATGCGAA GCAGCTACGG ACTGAGTGCC ACTATCAATC TCGAAGGCTC ATCAGCCAAC 
AAACTGCAGA CTTTGCCAAC GGTGACTTTG GTCCGCGATG CCCAAACGGG GTTGGAGGTT 
CCCACCTATG CCCAACTTAA CCAGAACAAC TCGCTGCTGA TGCCGGCCAC GTTTGTGCTT 
CGGCTGAACA AACCCATGCC TGTTTGCCTG GAATCACTAA AGGCTCTCGG TCTGCCCGGT 
CTAGACTCAG TTGCCACTCC TCCTGGTCCG CCCACCACAG TGCTTAATCT CATAGTACAA 
ACTGCATCGA AGCAGGCCAT TAAGAACACA CAGCGCGGCC TGTATGTGAA TTTACCTAAA 
GAGACGCACT GCTATTTCTT TACCGACAAC CGCAAACTGC AGGGAACTCT GGTGTCATCA 
CTGCCCTTCA CTGAACCCGC CCAGGTTCCG CGAATCGTGG CGTTCCTCAA GAAACAAGCT 
CTCTTCTACA CCCTGCTCGC AAGCTGTGTT CGTGAGCAGC AGAAGCAGTA TAATGATATG 
GACAGCACTG TGATACTGGA GGTAACCGCC GTTTCATTTA ATCAAATCAC GGTTGAGCTG 
CAGCACCCGT ACGAAGAGTC GCTGGCCACT GTGGATTTTC TGCTTGAGGA TGGTCAGCCC 
ACGTGCAGCG TTTACTGTCT GACCAACGAA TACGAGCTGC TGTCCCAGAA GTTAACCCGC 
ACCGCGCGCA AAGTGGTATC CATTCCGATG GTAATCTACA AGTTGCTTAA GTGCTGGGAT 
GAGGAGCACG AATTCAAACT GCATGGCGCG ATTGGACCTG GATCTGGTTC TGGAGCAATC 
GGAGGTGGAG GCATGAGTGG TGGTGGACCT GTCTCAGGAG TAGGCAATAA CTTTAGCCAG 
TTTTCAATGG ATACGCCGAC GCCTTCCGAT GGAAGCCTAC CCGGAGGTGG CTTCGCCAAC 
ATTAACAACC TTAAAATGGA TGCGAAGTCG CGATCTCTAG CGGATGCTTT TGCCGCTTCT 
ACTTCGGCAG CAGCGGCCAT AGCGGGTTTG ATCAATCTTA AGCGGGAAAC CGATCCACAG 
TCTGGTAGTT CGGCCAGTGG CACCACGGTC AGCGGCAGCT CCAGCTCATC CGGCTCCGCC 
AAGACGAGTG ACCATGACAT TGCTGACAAG TACAAAAATA TCTGGAAGGA CAAGACTCCA 
AATTTGAAGC ATTGCGTCAG CATCACCCCC ATCCCAGGAG ATGGAAAGTC TGGATCAGCT 
GGGGGAGTGT CCGGGGTCGA GGTACAGCGA ACGGGTGGTA TCGAAATTAT TCCATTGAAT 
GCCCAGGCGG CTATAGCCGG AGGAGGTGTT ACAGCCTCAT CCAGTGCCAC TCCCACGACT 
ATAACTATTA CCCCAATAAC TGGCAAGGAT CCCAGTAAGG ACTCAACCAA AAAAAGTACC 
GCTGCTTCAG CTGGAGTAGG TGTGGCCGCA AAGCGTCCCC ACGAAAGTAG CACAAGTAGT 
TCGTCTACGT CCGGTTGTAG TGGTTCTGGC AGCTCCATGT CGTCTTCAGC CAGCAGCGGC 
TCTTCAGATA CGCAGAAGGA AAAGAAGCGC AAGAAAAAGC GCGACGATTC GCCCATGGGA 
CCGCCGGAGA AGATCTATTC TAGGCAAAAC TCGCCTGCAG GTGGCGCAGA TGCTTCCGCA 
ACTGGTGGAG TGGTTCGCAA GTTTTCCTCT CCCTCGTCGT CACCCAAAGC TGGTGGCGGT 
GGACAGGGTC TAATGGCCGG AGTGCCGACT GCCCGTCCCA GCCCCAAGCA CTCTCCCGTG 
TATAGCAGTC CCAAGCACAA CACCGCCTCC AACAGCCCGA AGTCGCCGTT TGGCACACAC 
TCACCCAAAC ACGGCTCTTC CGGAAAGCCG AGCATGTCAA CGCTTAAAAG TGCAGCTACA 
GCTGCCACCA TCTTAAGTCC CAAGGGCGAT AAGTCCTCGT CAGCTGTGGG TAACACATCT 
TCGGGACCAT CCGCCTCCTC AGGATCCAGC GGAGCTACTG GCCTGGTGAG GTCATTTGCC 
AGCGTTGGAG CTCCGCCTCC ACCACCTCCA ATTCCTCCAT TAGCGAGCTC GAGCGGATCT 
ATCAGCAGCA GCCAGAGTTT AAAGAAGGAG AAAACCTCTT CGGCATCCGG TTCCAGTTCC 
ACATCATCCT CGGCAACTGC AGGTGTCGCA TCTGGCGGAG GTATTTCACC GGCAAGTGTG 
GCTGCTGCAG TAGCTGCCCT GAAATCCTCA CAACAGCAGA TGAAGTCAGT CGCCAGTCTT 
TCGCATCTGG CGGCGGGCGG GGGACTGGGT AGCTATGCGG CCCCGTCGGG AGCGGGAGCA 
TCTGGTGCAG CCGCAGTGGT GGTGGGAGCA GGAGCTGGCG CTGGAGCGGG GGCCTCCGGA 
CTGGAGCTGA GTGCCCTGCG CAAGGGCATG GCGGGAGGTG CGGTTAGTTT GATGACGTCG 
ACGGCAGCTT TAGCGCCAAC AATTCCGGCA CCAACAACAA CAGTGGCAGC GGGCTCGGCA 
GCGTCATTGG TGTCTCCAGT CTCAGCGGTA GTGGGTCAGG GGCAGGAAAC AGCGGGAGCA 
GCGGCAGCAG CAACGCTGGC AACAGCAACA ATTCTGCAGC AGCAGCCACA GCCGGGAGCA 
GCGCCAACTA GTAGCTGCTT AACCACCAGC GGGGGCAGCA GCGACTCCGC CGGGAGCATC 
AATCCGGCCG GAGCCTCCAC GGAATACATG GTGAAGCCCA GCAGCCAAGA GGGCCTCAAG 
CTGACCATCA ACAAGACGGG AAGCAGCAAG AGCTCCGGCA CCGGCTCCGG CTCTAGTTCC 
TCCTCTGGCC TCCAAGCGAA GGCAAAGAGC AGCAGCAGCG GCGCCACGAG CTTCGCTGGG 
TCCACTGGAT CCACGAAGAA GCAGCATACC GGACTCAAGC CGGGCGTTAA CAGCGGACCC 
GCTTCCAAGA AGGCCACCGC AGCGGTCTCG TCCGCCACGG CATCATCCAG CAAGCATTTC 
TTCCAGAAGG CAAACTCCTC GGGCAATCTC AGCAGCAAGC TCAGTGGGTC GGGATCAGGT 
GGCGGAATTC CGCTGACTAA GAGCAACAGC ACTAACTCCT TCCAAGAGCA CAATGCCCCC 
AGGCGGCGTC CCAGCATGGG AGCCTTGGCT AGCGGCAGCA GTGGAGGAGG CAGCGGGCAG 
CGAAAGTTAG GCTCCGCTTC GGGAGGAGGC AGCGGATCTT CGGGGTCGGT ATCGCCGGCC 
CTTAGCGGCT CAATGTCGCA GCCACCGCCG CGATTCGATC ACCACACGGA TATGATGACC 
ATCCTCCAAT ACGCTTCTCC GACAATGGCC GCAAGCATGG AAGGCTTCAT CAAGGGGCTC 
CACAACAAAT TCCAGATCCC AAAGTTATCG CAGAGAGGAA GCGGAGGCAA TACAACCAGT 
GGACGCAGCA CGCCAAGTGG CAGTTCAGAA CCGGCTTTAG CGGGAACATC ATCTTCGATA 
TTAGGACCTA TTGCCAGCTC TACTGGACTG ACGGAGCCGG AAGCGAAGCC GCCGGTGCCT 
CCTTCGCAGT CTGGCAATGA AGGACTGCTC AATCTTAGCA GCACGGCGGG TACACCATCG 
GCGGATGGGA TAGATGAGGA GCTTTTGGCT AGCCTGGCTG GCGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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MED1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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